LOCUS       DAOOIS010000001        87711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_288_length_87711_cov_39.094951, whole genome shotgun sequence.
ACCESSION   DAOOIS010000001 DAOOIS010000000
VERSION     DAOOIS010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 87711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 87711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87711
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_288_length_87711_cov_39.094951"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..251)
                     /locus_tag="PK960_00005"
     CDS             complement(66..251)
                     /locus_tag="PK960_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPD07598.1"
                     /translation="MLPGPPEETASTLTKMLVSVVENGYGKLAKVGGYHIAGKTGTSQ
                     IPYTSFGIDKASYSNET"
     gene            complement(237..319)
                     /locus_tag="PK960_00010"
     tRNA            complement(237..319)
                     /locus_tag="PK960_00010"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(283..285),aa:Tyr,seq:gta)
     gene            complement(328..403)
                     /locus_tag="PK960_00015"
     tRNA            complement(328..403)
                     /locus_tag="PK960_00015"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(368..370),aa:Thr,seq:ggt)
     gene            complement(408..1238)
                     /locus_tag="PK960_00020"
     CDS             complement(408..1238)
                     /locus_tag="PK960_00020"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD07599.1"
                     /translation="MFLMWLCIALLIIALAIVLFIIFHKMPLLANIDVDNLPAEQAAA
                     KKQEIINDRIRRQLAKHSWWWRNIVASLYTKIKEFFWRGYAKLNRLHRQQVKTKLQSR
                     GLNRERAKELLVDAEEALSQGRFSEAEHLALEALELDQSQIDSFAFLANVYNEEKKYK
                     EAQEVWHYIVKRLTQKYRAEELRRHPEAKNTKQKLAEYHYLLGQSYYRSDELERADHY
                     LRQALKLAPKNPRYLDTMLAVSIMRKDRVTAVTVFDTLSAVDPGNGHLPDYLEQIRQL
                     "
     gene            complement(1244..1984)
                     /gene="tpiA"
                     /locus_tag="PK960_00025"
     CDS             complement(1244..1984)
                     /gene="tpiA"
                     /locus_tag="PK960_00025"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPD07600.1"
                     /translation="MHKLIIGNWKMALGYKESVTLAENLVLSQPYRPLLAEVGVCPSF
                     VALSSVANILEGTAIFWGSQDATQFVPGPVTGGTSLESLVELDCRYVILGHSERRQLF
                     GETDELVRLKLQAVLERTNLIPIVCVGETLAEREAGQAEEKVGGQLRAIFNSVVVPAG
                     REVVLAYEPIWAIGTGKTSTPTQAEAMHHFIRQFLKELGIEGNRILYGGSVKPENAGQ
                     LLSQPNIDGLLVGGASLSADSFWSIVFA"
     gene            complement(2034..2921)
                     /locus_tag="PK960_00030"
     CDS             complement(2034..2921)
                     /locus_tag="PK960_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPD07601.1"
                     /translation="MELQRTIIILLVNLAWWLMAVIWSSQPLVLAGEVPLVWPNNWPE
                     VKAWPASSTVVISNKEGVLLSQGSYQLRPIASLSKLVTALIVLDTHPNWSAEYTLKNE
                     DQRYGNIIYIYPGEVVTIKDLWMASLLASDNTATEALIRAVGLDDASYSQKVEELARK
                     FNLASDLHLVEPTGLSPDNQASARAVATIVKTALSQSEIRETLLLEQYEIVTKQGRRQ
                     LVTNTDGFIRGSVAAPDSWQILGGKTGYLDEAGYCFAALWRNNQGEELITVVLGAPTK
                     EGRFTITKDLLNYITEKYY"
     gene            complement(2928..3863)
                     /locus_tag="PK960_00035"
     CDS             complement(2928..3863)
                     /locus_tag="PK960_00035"
                     /inference="COORDINATES: protein motif:HMM:NF039189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HPD07602.1"
                     /translation="MPTKKANSLPKFTFLYLLSLISLIFLAISAGVVAFQLINKWLPD
                     PLTGYTISYTASSLKFGLSALLISTPLYFWAVSQINRGLVKREISPQSDLRRWLIYFI
                     LLVTAIVMSGWLVALVYHYLDGELTGQFLAKAIVAIGIAATIFGFYHYDLHRNPQSPS
                     DKYHLHSFAWPVAIVIIGLIIGGFAIAESPTEARNRRYDEKLIGNFYQIDSALNVYYQ
                     ENKHLPATLQELTVSPYYLDPAALKTSEGEAIRYRGLGNNQYELCALWHTNNINPDKG
                     VRTVGVEKWPHEAGYQCIKQVIWEDSGGPVEQPLK"
     gene            complement(3868..5661)
                     /gene="aspS"
                     /locus_tag="PK960_00040"
     CDS             complement(3868..5661)
                     /gene="aspS"
                     /locus_tag="PK960_00040"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPD07603.1"
                     /translation="MLRTHTCGQLRATEVDQTVYVAGWVQSSRNLGGLMFIVLRDHSG
                     LIQLVVDLAQEPKVAVIAEELHDEFVVRAHGRVRLRPVEMVNPAMPTGEIEVVVDDLE
                     ILSRSLPLPFSLAEDKVGLSNENLRLEYRFLDLRRPSVQEMLITRDLFIRRMRQYFQD
                     LGFVEVQTPILANSSPEGARDFLIPSRLFPYHCYALPQAPQQFKQLLMIGGVDKYFQI
                     APCFRDEDGRQDRHYGEFYQLDMEMSFVDSEEDIWQIMEPLMLELTSEFSSKKVIGLK
                     DGRFPQLTWREAMETYGTDKPDLRYDLTIKPLSQVLAHSHLSIFQDVLASGGHIYGLV
                     LPETEKLSRRQLDELTALAKTKGVSGLSSLIYLGENWQGSLATKLEDNILLSLVKHLS
                     LKGGETILITAGPGLQACEALGAIREEIAGWFNLKDPGLAAWCWIYDFPFYEVGSTPT
                     GLNFSHNPFSLPKGGLEALKNQPPLEIVAYQFDLILNGVEVSSGAIRNHQPEVMYEAF
                     RLVGYEPAEVDRRFGALLKAFKYGAPPHGGNAPGIDRLLMALKDYDSIRDVYAFPKDT
                     QGRDLLMGSPSEVSQNQLDDLYLSWRQDTKK"
     gene            complement(5655..6362)
                     /locus_tag="PK960_00045"
     CDS             complement(5655..6362)
                     /locus_tag="PK960_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HPD07604.1"
                     /translation="MRRTTTERLLIIKRTEDRDYRGKIVALSPERGRLVFTAKGLFKP
                     QSKLSGHLEPLSLVDGLIVWSKEPLLSAAVSRQSFLGLKSDLALIVVSTELVRRVIEW
                     WPLAQPLPRSWQDLITILTQWNSHHYSLSQLTVGSQIMSWRLARELGYAPGHTCIQCG
                     RPLSTAFWSETQGGFFCEVCQPKGPTYKLSPSTLVIWEDLVQDEHLQLTKLPSSPTIL
                     EELTAFFNSWLNFLALC"
     gene            complement(6343..6744)
                     /gene="ruvX"
                     /locus_tag="PK960_00050"
     CDS             complement(6343..6744)
                     /gene="ruvX"
                     /locus_tag="PK960_00050"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPD07605.1"
                     /translation="MNEPIIFLGLDWGQKKIGLALGDSETKLAFPYGVVKDWPAVEAV
                     IKRENVDQIVMGEPYHLGGERGKYLKGWQELVELIKHSGYPLHLVDERLSSQGANRLI
                     NHQSRAAAPEDAIAAMLILQTFLDNYAAYDH"
     gene            complement(6747..7577)
                     /locus_tag="PK960_00055"
     CDS             complement(6747..7577)
                     /locus_tag="PK960_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPD07606.1"
                     /translation="MIRYKTPEQIALIKEGGHILAGITQKLAATVKPGVTTADLEALF
                     LELVAAAGGAPSFKGFRSSRRERPFPTGLCASINEQIVHGPAIPARVLHSGDIIGLDV
                     GMEYPLKKPGYLPSWPVNSSAPHGGYFTDMAVTVPVGKVSRLANHLIVVTRESLALAI
                     NEVAPGCSLLKIAQVIEGLVEQHGFAVVHNLVGHGVGLAVHEDPSIPHYVCNEYGEQD
                     IVLKPGMVIAIEPMVSAGSPEIKVGRDGLSFETADGSLSAHFEHTIAVTTTGHQILTI
                     "
     gene            complement(7590..8249)
                     /locus_tag="PK960_00060"
     CDS             complement(7590..8249)
                     /locus_tag="PK960_00060"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HPD07607.1"
                     /translation="MVRHILFFIGPPASGKGTQAELVSKYYGWPAISSGDLLRRSADL
                     PQKYQEAIQAGHLVDDGVINDLLQRRLTQADTKLGFILDGYPRNSSQFAYAYNYIITP
                     QDRVCALEIRVSDEEVYRRLEGRRFCPHCGAGYHLIYKPSKREGFCDVCSSKLVRRPD
                     DKPEVIAERLKIYHDTMEPLLALWQKRGELEVINGEQDIPAVHQDIIAVLTRRGYVLP
                     H"
     gene            complement(8380..9651)
                     /gene="secY"
                     /locus_tag="PK960_00065"
     CDS             complement(8380..9651)
                     /gene="secY"
                     /locus_tag="PK960_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPD07608.1"
                     /translation="MMEKILKIWRMRDLRNSILFVLAMLVVFRLAAHIPIPGVNAQAL
                     RELFASNQLLGMMNLLSGGGLQNFSIVMMGVAPYITSSIIFQLLGMIVPSIEEMNKEE
                     AGRQKINMWTRWATVPLAALQAYGMITLLRRSSYQVLGALDPLNFITIIITITAGTIF
                     LMWLGELITEKKIGNGISLLIFAGIIAGVPQSFQQIIATYNSSQFFMILGFIIVAILT
                     ILGVVYVTEGQRNIPVQYARQMHGGHSYGGSTTHLPLRVNMAGVIPIIFAVSILMFPS
                     MIAQFFVTSKVAWLATASAWVIKAIQDQWVYGIAYFILVVVFTYFYTEVVFHPDRVAE
                     NLQKQGGFIPGIRPGQPTARYLANIMHKITLAGALFLGVIAILPLIMRSITGVQALAI
                     GGTSLLIVVSVVIETVKQIEAQMTMRQYDGY"
     gene            complement(9655..10095)
                     /gene="rplO"
                     /locus_tag="PK960_00070"
     CDS             complement(9655..10095)
                     /gene="rplO"
                     /locus_tag="PK960_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPD07609.1"
                     /translation="MLTLNTLQPHSGAKKSRRRVGRGNSSGHGNYSGRGLKGQKARSG
                     VSDLKRRALRQNILQIPKLRGFHSDKPKAQVVNLSSLNHHFQDGAVIDPQSLVKAKLV
                     KSGQPIKLLARGELKLKNLRVRNLKVSAAAKEQLTKLGGQLDNK"
     gene            complement(10108..10731)
                     /gene="rpsE"
                     /locus_tag="PK960_00075"
     CDS             complement(10108..10731)
                     /gene="rpsE"
                     /locus_tag="PK960_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPD07610.1"
                     /translation="MKDIKATISPNAEPRNNRQRRFSTSRGQGGRSEQQPDEFDQKII
                     DIARVTRVMAGGKRMRFRACVAIGDHKGRVAIGLAKGLDVTTAVNKAVNQAKKSLITV
                     PIINGTIPHEVRQKSDAAIIILKPATQGKGIIAGSVVRIILELAGLHNVTSKMIGSHN
                     KVNNAKCTMKALQSLKKPVRQDKQDKNVATADSAKSSKKVVPAAAKA"
     gene            complement(10744..11091)
                     /gene="rplR"
                     /locus_tag="PK960_00080"
     CDS             complement(10744..11091)
                     /gene="rplR"
                     /locus_tag="PK960_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014215718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPD07611.1"
                     /translation="MHSEKRVNKIRRHKRVRAKIKGTGTCPRLSVFRSNRGMYLQAID
                     DTTGQTLVSAHSREIKLAGTKTEISRALGKLLAEKLLAKDIKQVVFDRGGNAYHGRVQ
                     AVAEGAREAGLQF"
     gene            complement(11106..11645)
                     /gene="rplF"
                     /locus_tag="PK960_00085"
     CDS             complement(11106..11645)
                     /gene="rplF"
                     /locus_tag="PK960_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPD07612.1"
                     /translation="MSRLGKLPIILPSGVTAMMQAGILTVTGPKGQLQQAILPEVQVT
                     IDQDKILVNVANREDRKERALWGLFHRLIGNMVIGVTKGYEKKLEINGVGYRVNVAGQ
                     KLTFSLGFSHPVEFNLPSGITATVDGNVITIAGQDKQLVGEVAAQIRRLRPPEPYKGK
                     GIKYADEVIRRKAGKTAAK"
     gene            complement(11671..12084)
                     /gene="rpsH"
                     /locus_tag="PK960_00090"
     CDS             complement(11671..12084)
                     /gene="rpsH"
                     /locus_tag="PK960_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPD07613.1"
                     /translation="MIDPISDMLTRIRNAAAVHKTEVILPMSKLKYNIAKILEREGYL
                     AAVEIINPPLTKNKTANFASLKLVLKYQANGRPAITSIKRISRPGRRVYASKDKLPVV
                     VNNFGIAIISTSAGLLTNKEAREKGLGGEVLCSVY"
     gene            complement(12118..12303)
                     /locus_tag="PK960_00095"
     CDS             complement(12118..12303)
                     /locus_tag="PK960_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPD07614.1"
                     /translation="MARQALIAKAKKTPKFSTRVVRRCWRCGRNRGYMRDFGLCRICF
                     RELASSGDLPGITKSSW"
     gene            complement(12319..12864)
                     /gene="rplE"
                     /locus_tag="PK960_00100"
     CDS             complement(12319..12864)
                     /gene="rplE"
                     /locus_tag="PK960_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPD07615.1"
                     /translation="MSMRLSEKYKQEVIPALMEQFSYSNKLSVPRLTKVVVNVGVGRM
                     AKDKAFIDGVQDTLTKITGQKPILNPARKSISAFKIREGAIVGVSVTLRGQRMYDFVE
                     KLIAISLPRIRDFRGLDSGVVDQQGNLNLGFKECSSFPEVRLDELEKEHGLQVSIATT
                     AKTHEEGLALFKLLGFPFKAL"
     gene            complement(12872..13204)
                     /gene="rplX"
                     /locus_tag="PK960_00105"
     CDS             complement(12872..13204)
                     /gene="rplX"
                     /locus_tag="PK960_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPD07616.1"
                     /translation="MKIKKNDQVLVLSGKDCGKRGKVLQVFPKEQRASVEGINLLIKH
                     IRPQHRGEVGQRIEFPAPLHISNLELICPHCGKPTRVGYQYIKVTEGQKAQNRKVRIC
                     RRCKEFID"
     gene            complement(13215..13586)
                     /gene="rplN"
                     /locus_tag="PK960_00110"
     CDS             complement(13215..13586)
                     /gene="rplN"
                     /locus_tag="PK960_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPD07617.1"
                     /translation="MIQVQTMLNVADNSGAKKVQCIRLMKGYRRHYASVGDMIVVTVK
                     EAMPHTSIKKSDIVPAVIVRTTKEIRRSDGTYIRFDDNACVIVDKKTHEPRGTRIFGP
                     VPREIRRAGFVKIASLAPEVL"
     gene            complement(13599..13832)
                     /gene="rpsQ"
                     /locus_tag="PK960_00115"
     CDS             complement(13599..13832)
                     /gene="rpsQ"
                     /locus_tag="PK960_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPD07618.1"
                     /translation="MHKKFQGIVVSDKMEKTLVVRVERVKLHPKYHKRYVVSRNYKVH
                     DDSGLYHKGDRVSFVECRPLSKEKRWRVIKNGR"
     gene            complement(13909..14100)
                     /gene="rpmC"
                     /locus_tag="PK960_00120"
     CDS             complement(13909..14100)
                     /gene="rpmC"
                     /locus_tag="PK960_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPD07619.1"
                     /translation="MNYHELQEKTDTELRLLLAEKRVALKDLRFKAASGQLKSVREIR
                     ATRELIARLLTLLNNRQKK"
     gene            complement(14109..14525)
                     /gene="rplP"
                     /locus_tag="PK960_00125"
     CDS             complement(14109..14525)
                     /gene="rplP"
                     /locus_tag="PK960_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013324982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPD07620.1"
                     /translation="MLMPKKTKYRKSHKAKRGGVATRGTTVSFGSYGLKATTACWVTS
                     RQIEAARRVITRYVQRTGKLWIRVFPDHPVTAKGGEIPMGKGKGSVDHYIVPVKPGTI
                     MFEIEGIDESKAREAMRLAAHKLPVKCQFVSRDQVN"
     gene            complement(14543..15232)
                     /gene="rpsC"
                     /locus_tag="PK960_00130"
     CDS             complement(14543..15232)
                     /gene="rpsC"
                     /locus_tag="PK960_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPD07621.1"
                     /translation="MGQKVNPTIFRMGIIKTWPSKWFKGGKSYINNARQDIKIRDYLF
                     RQLREAGVDRVEIERSANKVAINLFVAKPGLVIGRGGAGAEELKKTLHNRFLKNFRLH
                     DITLNISEVNRPNLSAQVLMQSMVMDLEKRMPFRRVMKQTINRAERSGALGIKVMVGG
                     RLNGAEIARTESLTSGKVPLHTLRADIDYAKGTAHTTYGTIGVKVWVYRGEVFGRTEE
                     VKGEVVGQRLR"
     gene            complement(15465..15791)
                     /gene="rplV"
                     /locus_tag="PK960_00135"
                     /pseudo
     CDS             complement(15465..15791)
                     /gene="rplV"
                     /locus_tag="PK960_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872951.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
     gene            complement(15798..16088)
                     /gene="rpsS"
                     /locus_tag="PK960_00140"
     CDS             complement(15798..16088)
                     /gene="rpsS"
                     /locus_tag="PK960_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPD07622.1"
                     /translation="MTRSLKKGPYVNERLLAKIKKLPLGDKTIIKTWDRACTISPEMV
                     GFTIGVHNGRQHIPIFITENMVGHKLGEFAHTRKFLGHGGKMARSQTKGATK"
     gene            complement(16109..16948)
                     /gene="rplB"
                     /locus_tag="PK960_00145"
     CDS             complement(16109..16948)
                     /gene="rplB"
                     /locus_tag="PK960_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPD07623.1"
                     /translation="MAIRQAKPITPGLRGASYDSFSDITSTTPERQLLVIRKKHSGRN
                     NQGKITVRHRGGGSKRYIRLVDFKRDKFDIPGRVKAIEYDPNRGARLALVYYLDGEKR
                     YIIAPLGLKVGDEVLSSKKLIDIKTGNALPLEYIPAGVPVHNVELRPGFGGKLARAAG
                     NVLYVMGVDKRYAQLKMPSGEIRLVDKNCLCTIGQVSNPELARINLGKAGRTRHLGIR
                     PTVRGTAMNPNDHPHGGGEGNQPIGLKYPKTPWGKHALGVRTRNPKAANNALIIRRRQ
                     KKH"
     gene            complement(16967..17347)
                     /gene="rplW"
                     /locus_tag="PK960_00150"
     CDS             complement(16967..17347)
                     /gene="rplW"
                     /locus_tag="PK960_00150"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPD07624.1"
                     /translation="MDKRNKINKTTESNETQGEAQIIKTDRQTLKRGQLASILIKPAV
                     TEKAGTLNELNQYVFVVERGANKITIAKAIEAFYGVKPIAVNIINMPGKKVTRGRIKG
                     QRPAWKKAIVTLPAGKSIAIYEGV"
     gene            complement(17354..18019)
                     /gene="rplD"
                     /locus_tag="PK960_00155"
     CDS             complement(17354..18019)
                     /gene="rplD"
                     /locus_tag="PK960_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPD07625.1"
                     /translation="MIKYKVYNQGAEVIGEQELSEAVFGVALNKGLVHQAMVAQLANA
                     RRVIADTKDRGEVSGGGKKPWRQKGTGRARAGSNRSPLWIGGGVTFGPTKDRNFSQKI
                     NKKMAARAICMVLSDRVATNNLIILDSLTVPEGKTKALKEIIDKLLAAQESDEVKKHS
                     QVLLITTVDNESIKLASHNLPHLRVLNDQNINLVSLLRAPKLIITQPVIDILQARYGQ
                     NVN"
     gene            complement(18032..18775)
                     /gene="rplC"
                     /locus_tag="PK960_00160"
     CDS             complement(18032..18775)
                     /gene="rplC"
                     /locus_tag="PK960_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPD07626.1"
                     /translation="MKFILGKKMFMTQLWRDDRVVPVTCIKVEPCVITQIKTRERDGY
                     RGVQVGTGRRAVKNIAKPQRGHFGAWGNFQYQREFNLDREPAGVEAQLQVGQHIAIET
                     FVPGDLVQVTSVSKGRGFQGGVKRHGFQGQGRSHGDKDQQRMPGSVGATGPAHIFKGT
                     RMPGHYGSQQVTVKNLEVVEVDVANGYLYLRGAVPGWRNGLVAISGSGELITKTDQAP
                     VELEETKTEPEVNEELVVDNQEKSKVEVA"
     gene            complement(18990..19325)
                     /gene="rpsJ"
                     /locus_tag="PK960_00165"
     CDS             complement(18990..19325)
                     /gene="rpsJ"
                     /locus_tag="PK960_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018298950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPD07627.1"
                     /translation="MSDKKVASSEYKQRIRIKIRAFDHKIIDQSTKTIIETVERTGAK
                     IYGPIPLPIEKKKYTVNSSTFVHKNSRDQYEMRIHKRLLDIIDPASKTVEALMSLSLP
                     AGVDLEIKM"
     gene            complement(19394..20584)
                     /gene="tuf"
                     /locus_tag="PK960_00170"
     CDS             complement(19394..20584)
                     /gene="tuf"
                     /locus_tag="PK960_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPD07628.1"
                     /translation="MMTEKYERTKPHINVGTIGHIDHGKTTLTAAILSVLTAAGYKAT
                     QKSVDQIDSAPEEKTRGITIATTHVEYETAKRHYAHVDCPGHADYIKNMITGAAQMDG
                     AILVVAATDGPMPQTREHILLAKQVGVPYIVVFLNKCDMVEDKELIDLVEEEIRDLLK
                     KYGFPGDTTPIIRGSALQALQNPTGEYAKPILELVDTLDNYIPESQREVDKDFLMPIE
                     DIFSIEGRGTVVTGRIERGTVKVGDEVELVGLGETRKVVVTGVEMFKKTLDQGMAGDN
                     AGILLRGIKKDEVQRGQVLAKPGTITPHTEFEGEVYVLTKEEGGRHKPFFNGYKPQFY
                     IRTTDVTGEIQLPEGTEMVMPGDTVTLKVKLIAPIAMDEKMRFAIREGGRTVGAGVVT
                     KIIK"
     gene            complement(20646..20963)
                     /locus_tag="PK960_00175"
     CDS             complement(20646..20963)
                     /locus_tag="PK960_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07629.1"
                     /translation="MINNLEQILRLAQLTGDRLIVADQENPVSSLVILSLSDYEKLLD
                     LAGLTNGQGFAKVSTNVASSDTGNISVKSSFVEQVDDRQKSHWSIPASRKSAAKDLAG
                     EDK"
     gene            complement(20986..23061)
                     /gene="fusA"
                     /locus_tag="PK960_00180"
     CDS             complement(20986..23061)
                     /gene="fusA"
                     /locus_tag="PK960_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPD07630.1"
                     /translation="MREFKLEDVRNIGIMAHIDAGKTTFTERVLFYTGKKHKIGETHD
                     GEAEMDWMAQEKERGITITSAATTCFWKGKQVNIIDTPGHVDFTVEVERSLRVLDGAI
                     AVFDGAAGVEPQSETVWRQADKYKVPRLCFVNKMDKMGADFVMSLNSIRERLNPRAVA
                     LQLPIGAEETIRGVIDLITEKAFEFHGQCGEQIVEVAIPDNMREEVTKYHHELIEKIA
                     ELDETLIDKYLNGEEISIEELKRALRQEVIANEIYPVFCGAALKNIGIQPVLDAVVDY
                     LPSPLDLPDVEAHDANDPNKVIIVKPDDNGPFVGLAFKIATDPYVGRLCFVRVYSGIL
                     KAGSYVINAANGAKERIGRLVRMHANHREEITEICAGDIAAVIGLKETVTGNTLCDEG
                     QRICLEPIVFPEPVIQIAVEPKTKADQEKMSVALVRLAEEDPTFRVETDEETGQTLIS
                     GMGELHLDIIVDRMKREFGVEANVGQPQVSYRETIRQTAEAEGKYIKQSGGRGQYGHC
                     WLRLSPQPEGQGSEFVDEVKGGVIPKEFIPAIEKGFREALDKGVLAGYPMVDIKCTVY
                     DGSYHEVDSSEIAFKMAAILAFREACKKAQPVLLEPIMKVEVITPEEYMGNVIGDLNA
                     KRGQIDRMFDRGQLKVIDARVPLAEMFGYATQLRSISQGRASYNMEFLHYREVPRNVA
                     EVIIGKTSK"
     gene            complement(23148..23618)
                     /gene="rpsG"
                     /locus_tag="PK960_00185"
     CDS             complement(23148..23618)
                     /gene="rpsG"
                     /locus_tag="PK960_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPD07631.1"
                     /translation="MRGKQAQKRKIAGDAKYNDPEVAKFINYVMRNGKKTVAEQIVYD
                     CFDILKEKTKQEPRHVFNKALKMVCPLVEVRGRRIGGANYQVPYQVRGDRRFTLGCRW
                     LITAAKARKEHSMAEKLAAEILDSIKGEGAAVKKKLDVHRMAEANKAFAHFARH"
     gene            complement(23641..24057)
                     /gene="rpsL"
                     /locus_tag="PK960_00190"
     CDS             complement(23641..24057)
                     /gene="rpsL"
                     /locus_tag="PK960_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPD07632.1"
                     /translation="MPTINQLIHKPRKVVGHKSKTPALQVTTDSLHRRKNIAKKGSPF
                     KRGVCLKVTTTTPKKPNSALRKIARVRLSNGMEVTAYIPGIGHNLQEHSIVLIKGGRT
                     KDLPGVRYKVVRGVYDAQGVENRRQARSRYGAKAPK"
     gene            complement(24165..24761)
                     /locus_tag="PK960_00195"
     CDS             complement(24165..24761)
                     /locus_tag="PK960_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPD07633.1"
                     /translation="MMNLFLIIILLLSTIVHEYSHAWVAARLGDDTATRAGRLTLNPL
                     VHLDLFGSIILPGLLLFVGSPVVFGYAKPVPINFNNLRGGYKGVAKTALAGPAANFIL
                     AIVAAVATRYLAGGPNSDWGQLLFLAAMINVVLMVFNLLPIPPLDGSKILALVLPERW
                     RYSYLASERWSLLILLIVIIVFPDLIYYPVTWLMNLLI"
     gene            24869..25057
                     /locus_tag="PK960_00200"
     CDS             24869..25057
                     /locus_tag="PK960_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013243233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HPD07634.1"
                     /translation="MAKKCDICQRSATKGASRSHSNIKTLKRQGINLQNYKIGGQTVK
                     ICTSCLRTAKKVSQKKTS"
     gene            complement(25119..25191)
                     /locus_tag="PK960_00205"
     tRNA            complement(25119..25191)
                     /locus_tag="PK960_00205"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25156..25158),aa:Thr,seq:tgt)
     gene            complement(25350..25425)
                     /locus_tag="PK960_00210"
     tRNA            complement(25350..25425)
                     /locus_tag="PK960_00210"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25390..25392),aa:Lys,seq:ttt)
     gene            complement(25476..26225)
                     /locus_tag="PK960_00215"
     CDS             complement(25476..26225)
                     /locus_tag="PK960_00215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD07635.1"
                     /translation="MSGNSRPSLDLCLPVYNEEAILRDNVRRLIDFITAKDYGLDCHV
                     VILVNGSSDKSAEIAQQLSKEYPGLVESRVYPLAGKSRALLNYADTSLTDWWGFIDVD
                     LSISFESLAKLFAVIVNKSADLVIASRFLPGASCKRAFYRGIVSALYNKLARHVLGTT
                     VKDHQCGCKFVSHELWRAVRPFLRESDYFLDTALIAWTERLGWRIKEVPVDWVDNRYG
                     KRPTKIKLAKDTVNFLMSLKHLRRDLKSYRE"
     gene            complement(26212..27837)
                     /locus_tag="PK960_00220"
     CDS             complement(26212..27837)
                     /locus_tag="PK960_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07636.1"
                     /translation="MPQKTTKRFSFSRWLIILLFFFGLGLVLHNSFGYLDPDFGWHYA
                     VGQEIWQNHAVPHLEHHLFPLAGQSWVDHEWLSNVLIYLTYNWGGYLLINFLFAFLTM
                     FIWCLLAKETIKHWLDNKANSLIFWLLLLIGLAASLPHFGVRVQEITVLFVLVLTIWL
                     AQVVRKREWPTWKIVVLPLFFWLWASLHGGFLIGLAIFTAWFFFRLILYWLPHQSNKE
                     INILPKGVIMRSGLTLALSWVATCLTPYGLELYEFLGGYGSNKAYLRMINEWLPISEA
                     IHSWQMVYFIIFGLALALLIFYCCGHDQHDREVALWLKQQDWWTLILSLILCIAAWCS
                     VRHFPLFVVVSLPWMAGFFYHAWHDNAFRPDDWPAYLKWFVAIILVLTTVLLVVDAHW
                     STHPEKDYCEVYPCLAISYLQQSPYANWPTIVEYGWGGYFYWRWPEKPIFIDGRLPQY
                     MWRGQSLIEHYGRFFKEKEVKKALDDYGLDVMVIPQPRPVHYNWVETCLLGRGLKEED
                     YYTLSDIVRRAMLKSPAWRLVHSDTTTMIYVRQ"
     gene            27941..28213
                     /gene="rpmA"
                     /locus_tag="PK960_00225"
     CDS             27941..28213
                     /gene="rpmA"
                     /locus_tag="PK960_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPD07637.1"
                     /translation="MAHKKAGGSTSLGRDSQSKRLGIKLQDGQTAKAGAIIVRQRGTK
                     YRAGKNVAVGHDYTLFATKNGQVKMQTKKIRRFDNQLKVTKIVHVE"
     gene            complement(28257..29537)
                     /locus_tag="PK960_00230"
     CDS             complement(28257..29537)
                     /locus_tag="PK960_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPD07638.1"
                     /translation="MSSSSTSRRKWRWLLVLFLFLIALAIGFWSGILYDEKAQIVRRL
                     SDPQIVFLGKVTGLYNSDTGGRLSSDLNFDLFWQTWDMIKSNYVDHDQLTDKQMFYGA
                     LKGLVGSTKDPYTVFMDPEDAKMFNEDLAGSFSGIGAEVGLRNDVITIIAPLADTPAE
                     RAGLRAGDKVYAIDQQSTAGLTVDEAVKKIRGPKGTSVTLTIGRGDAKLQDITIVRDN
                     IVVKSLDYSYDSATGIYTITISNFNADTEALFATAVEDIKVKKPKGLILDLRNNPGGY
                     LDTAVAIASYWIESGVVVSEQSATKPPRQHLARGLAPLSNLPTVVLINQGSASASEIV
                     AGALMDYGQATTIGKTTYGKGSVQMVNDLPDGSMVKITTAKWLTPSGTSINDKGITPN
                     IEVDFTEEDFNANRDPQMEKAREFLLEKFKSNNE"
     gene            29634..30896
                     /gene="murA"
                     /locus_tag="PK960_00235"
     CDS             29634..30896
                     /gene="murA"
                     /locus_tag="PK960_00235"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPD07639.1"
                     /translation="MSKFIIQGQAPLKGQIKVNGAKNSALKIIPASLLSEESITINNL
                     PDIEDINRSLEIIKAIGGQINRQDPHQCSICTKNIHSTSIPSELANKFRASIMFVGPI
                     LARSGEVSFPHPGGCVIGAGIRPIDLFLEGFAALGATIHITNNYYHLSAKKLKGAEYF
                     FTTVSVTGTEALMMTATLAQGQTTLKNCAMEPEIVALADYLNAQGAKISGAGTPTITI
                     EGTNYIRGGHFTVIPDRIEAGTFAILAAVSQAELLITNCNPDHIAILLNIFHKIGVHF
                     EQGQDWLKILPSPKITAYNIKTHEYPGFPTDLQSPYTVLMTQAHGLSIIHETIYDRRL
                     LFTDMLTQMGANIIMCDPHRVVVNGPTPLHGRKLTSPDLRAGMAMVIAALIADGTTEI
                     DNIYQIERGYEAIAERLQTIGANIHKID"
     gene            30898..31761
                     /locus_tag="PK960_00240"
     CDS             30898..31761
                     /locus_tag="PK960_00240"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPD07640.1"
                     /translation="MSPRQRWWLSKIFILLFFLITPPILIYANGYRFNWHSWKLERTG
                     LLEIKSEPSNASVNINELKAWWGKPKKIVTPVKLKSLRPGNYNVSLSLPNYYEWHKTL
                     SVWPAQTTIVKDIRLFKESKPQLLATLTPSTTLTSATLDNHQLNLATKNLTTTTISAL
                     IDQEKLAKNWLDTTSIKLACPLPNGKIAFSNQFELWLANPDEAKTELLGRFSQPITQL
                     ICLPNTSNYLIFGQDSGWKIIEVNNDQPHLMWEIAHTDHLGAGSLDKKANTLYFYAQI
                     GSTTGLFSLEL"
     gene            complement(31756..32625)
                     /locus_tag="PK960_00245"
     CDS             complement(31756..32625)
                     /locus_tag="PK960_00245"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HPD07641.1"
                     /translation="MRQITKAILPVAGFGTRFLPATKAQPKEMLPIVDKPAIQYIVED
                     AVSAGIKEIIFVTGRGKRAIEDHFDYSFELDKILVEKNKLELVKEVRRISELAKFSYV
                     RQPLPLGDGHALACAAHLIYDEPVLVMFGDTLYDAKEGPASQILKTFAKYGDPVIGLS
                     AVPRNEVSKFGVIDGVAVGERTYEIKRFIEKPSPSEAPSNLVAVGIYVLTPEVLSWLT
                     TMKPGPNGELRLADAFQQMLAHDRPIYGQEFNGTWLDTGDKLSFIKATIQLGLKNEEI
                     GPELKKFIRTLSL"
     gene            complement(32631..33455)
                     /locus_tag="PK960_00250"
     CDS             complement(32631..33455)
                     /locus_tag="PK960_00250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD07642.1"
                     /translation="MDISIVIVSYHSQAVIYRSLESLRKTEWHDLEYEIFVVDNATEA
                     DLAQELATHWPEVHYIPSNNIGYAAGNNLALRQASGKYFVVMNPDTAVEPNSIWELYN
                     FMEHHPQVGMVGPRQYNPDGDRQESCYRWHRLYTPFCRRSPRLAKFSWAQKELRRFLL
                     ADVDLNQFCPVDWLLGSFLFIRTTAMRQVGYFDERYRLYFDDTDLSRKMWRHGWQVVY
                     YPEVAILHDHQRASAQMPWYKILRSWAGRQHVLSWFKYLLKWGPGGKSHVPRGKNI"
     gene            complement(33458..34537)
                     /locus_tag="PK960_00255"
     CDS             complement(33458..34537)
                     /locus_tag="PK960_00255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD07643.1"
                     /translation="MMNYKIAIIIINYKDYANKYLADCWESLSRLNLITPDWQIEATT
                     FIVDNQSDEASYTTLQTMAPTAYIIRNTNNDGFAKGNNDALALAMSWGYDFGWVLNMD
                     TIVEPDSLIELVKLMISHPKIGGAQSLLKWWPDKDLVNSLGNVTHFLGYGYCNGYKKR
                     VAELNLDELNGQPISYPSGASVLFRLSALRQVGLFDETFWMYNEDQDLGWRLWLAGWE
                     CRLAAYSMVYHKYEFSRSVSKYYWLDRNRLLSAWKNYHFLTLLVLLPALLLSEIVLVV
                     VYCCSGRLIYKLKAWWHFCNPLHWPSLFKARIRSQRLRQVRDHLVIKTFSGRILFQEV
                     DSPLVRVGNWGLNLYWQIIKLIIIW"
     gene            complement(34542..35405)
                     /locus_tag="PK960_00260"
     CDS             complement(34542..35405)
                     /locus_tag="PK960_00260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD07644.1"
                     /translation="MLDLAIVIVSWKVKDKLAANLKELLASQGNFNFEVWVVDNNSGD
                     GTVEMIKHDFPQVHLIANPDNRGFARANNQAIREIRARYVLLLNPDMLVRPDTLANML
                     AWCDANPQATVTGCHLVTENGETLLHVRRFPTIWDQLAIVLKLPHLFPHVLDHYLRTD
                     FDYTQAAPVDSVRGGFFWIRADREGRVPLFDERYFLWFEEVDYCRQVKLNGGQVWYTP
                     VATCVDYVGQSFKQVGRGQAQTYFRESMIKYFAKWEPRWQSRLLKLAWPIGIFMAVLG
                     EKLNWHRPAKT"
     gene            complement(35416..36444)
                     /locus_tag="PK960_00265"
     CDS             complement(35416..36444)
                     /locus_tag="PK960_00265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD07645.1"
                     /translation="MARGSRVYVAFLGYGQSTAKYLPKFLDSLRQQTFTDIEIIAGDN
                     TPSLDDNPNELIIKNYPAIRYYPFGANLGFAKGYNRLFKLAMEEEADYFLVINPDTLL
                     QPKVIECLVNYLDEHSEVGAAMPKILRWDFQGQHLTQEIDSLGLALTKGGSFYDIGQG
                     QLSGRELNYTHSVFGFTGAGVLLRLSALRSVAFDNGQYLEYFDELMFMYKEDCDLSYR
                     LRLANWDIVLVPEAVIYHDRTAVKARGGWKSQWKERRQSSKLIRRWSYHNQLVIFLKY
                     WSLPKKLSTRLKEVNYQLKRLAVAVMLEPFTLSVWKAIFKERAAIIARRRALNIKINP
                     LSLEKWRY"
     gene            complement(36449..37501)
                     /gene="pyk"
                     /locus_tag="PK960_00270"
     CDS             complement(36449..37501)
                     /gene="pyk"
                     /locus_tag="PK960_00270"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPD07646.1"
                     /translation="MNTRIIATIGPSSWDYNVLKSLIRNGADIVRLNFSHADIKQLVD
                     LKKNLKKIKQETGRQALIMQDLQGPRLRIGQLDQEIELIDGEVYTFTAGPSSVANREI
                     QIDDPDLYKDLKGGDLFFLSNGELELKVVSIKDKKIYARVERGGLLLSHKGINLPNTR
                     LRRGGLTPKDITDVEAALKCGGIDYIALSFVQSATELRKLRKIIAGRPIKIIAKIERA
                     VALENIDEIIKESDGIMVARGDLGIEVPLEDLPIIQKNLIRHAHWHGQPAIIATQMMT
                     SMIDHPRPTRAEVSDIANAVLDDADGLLLSDETAVGQYPARAVAIMEKIIRRTEKYLN
                     NKNYFDDEMVAFYRHP"
     gene            complement(37521..38246)
                     /locus_tag="PK960_00275"
     CDS             complement(37521..38246)
                     /locus_tag="PK960_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPD07647.1"
                     /translation="MRGIILSGGSATRLRPCTKVTSKQLLPIYNRPMIYYPLNTLIKA
                     GIKEILIIVSPERAGDYLNLLGSGRQFGVKLTYEIQDKPRGLAEAFIIGENFIDEESV
                     TMILGDNIFEDDFTDDINNFKGGAKIFAKKVKDPERFGVVEVNNKMEALSIEEKPLQP
                     KSNLAITGLYIYDHRVINVAKKLKPSVRGELEIVDLHNWYLARHELEVSLVRGAWIDA
                     GTFDSLLDAQILARDRLQNKLII"
     gene            complement(38251..39123)
                     /locus_tag="PK960_00280"
     CDS             complement(38251..39123)
                     /locus_tag="PK960_00280"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HPD07648.1"
                     /translation="MKVLVLGGQGNLGTQLTQSGSSWGYEIISWDREDVDLSNWPLTQ
                     ARLDSLSNLEIVINAAAYNAVDAAETEPGKTMADILNHQLVANLAYWCRERGLTLVHY
                     SSDYVFGGDKPGGYHEADEPQPINEYGRSKRAGEESLIAEGEQGLSYYLIRTSKLFGP
                     RGLSPNAKPSFFDIMFQLAATSPELKGVDAEVSCFTYTPDLAQATWQLLASRPASGIY
                     HIVNSEPTTWYGALQKLGELAHLTLPIKAISSADLPRPAARPQYSTLLMTKLSGLRPY
                     YEALAEYLSQLSKN"
     gene            complement(39120..40091)
                     /gene="rfbB"
                     /locus_tag="PK960_00285"
     CDS             complement(39120..40091)
                     /gene="rfbB"
                     /locus_tag="PK960_00285"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPD07649.1"
                     /translation="MKLLVTGGAGFIGSNFIHYWLKKHPADEIINLDLLTYAGNLANL
                     ADVADQKNYRFVKGDIASYEVVDELVGQVDCIVNFAAETHVDRSINNGLVFVKTNTEG
                     TANLLEAARQHGGVRFHQVSTDEVYGDLGPNDPKFNELSPYRPSSPYSASKAAADHLT
                     RAYYRTYNLPITISNCSNNYGPYQYPEKFIPLFITNLIEDRKVPLYGQGTNIRDWIHV
                     DDHNAGLEAILLHGRIGETYCLGGNHEVSNIELAKEIIRLLGKDESYIELVTDRPGHD
                     WRYSIDFHKAKEELGWEPRIDFASGLKDTVEWYKTHEAWWRPLKARI"
     gene            complement(40118..40579)
                     /locus_tag="PK960_00290"
     CDS             complement(40118..40579)
                     /locus_tag="PK960_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HPD07650.1"
                     /translation="MIQGVVIKHLTPHEDERGWLIEIYRDDEDHYQPVMGYVSVTHPG
                     VARGPHEHKYQSDCFVFVGPGDFEFHVWDRRENSSTKGQYMKEIVGQSNPVMIIVPPG
                     VVHGYKAIGDHDAWSINLPDKLYRGTNKVEEVDEIRWEQIMSFLMMGQDNK"
     gene            complement(40595..40786)
                     /locus_tag="PK960_00295"
     CDS             complement(40595..40786)
                     /locus_tag="PK960_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07651.1"
                     /translation="MKFKWQSCLKLNDFFYNWLLVGLVLFLVSEAILPGFVQPYINLN
                     LWLLFWATSAILEMLLVNK"
     gene            complement(40788..42218)
                     /locus_tag="PK960_00300"
     CDS             complement(40788..42218)
                     /locus_tag="PK960_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07652.1"
                     /translation="MFKGWRQLETWQKILWLGLIVVWLVLLYLAIRPTGVAIYRVNDW
                     SGTYFLHKFGPPERWATSSGGLVMIGEPGYFYVRPTRRFNQAEITIKWRKSSDVPLEV
                     GVLVDRAHWQYITKPLNNPVLNNLHWPSLMNGSYCLWQRKPVYSSWQDFINNPPPASQ
                     LALYNFSWPLAITPILDNYQAKANWQTLPGKWRGSQHLIVYVGPGESLAWRLTAVSDS
                     VKPLTADEREIEVNIYGPDSTRIQSSRFSLEQEQVSQEIIIPSKVAAVYNIEIKASSK
                     ILFTLATKQSIFSWQNQVWPVFSPDFSWQMWTDVPELTAQTIQPTSCETINVGSQSLT
                     IDSTYKQYHIITEDPVTEIKGRGGDIQFSGNGVFSLSSEVFNPYPRRVDRFLDKDPDI
                     NFVAANYEMPSCNEQICKASAVLPLKEAFWQPNDGYQFIIAVSGGQLSTSSPLTIEEI
                     QVKLIGSNLWQAIEHKLQFLWLKLTR"
     gene            42305..43171
                     /locus_tag="PK960_00305"
     CDS             42305..43171
                     /locus_tag="PK960_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPD07653.1"
                     /translation="MMRRKLSWWLLWLLAVILIIVIGYYNRHLVVTNYKISDPRWSAE
                     LNGWRLVQLADLHNQATDGPGNNKIINAVQKLQPNIIVITGDLFDGDKTNIPNNLRLV
                     KDLSSIAPVYIVLGNHEFANDDYIKLIQLVEEAGAQVIRNEVITINDKGIPITIIGLD
                     DPAAWGWPLSYKTQTQLTAQTLNKIMTSPDFNSTSPRLILAHRPELWPQYLLANPLII
                     LSGHTHGGQIRLPLIGAIYVPNQPLLAQYDEGIFAIANSYLIISRGLGNSFINCRFNN
                     YPEIVTVDFFHE"
     gene            complement(43162..44010)
                     /locus_tag="PK960_00310"
     CDS             complement(43162..44010)
                     /locus_tag="PK960_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPD07654.1"
                     /translation="MSSSGLGRGLESLLTSNPAATPIDSERITYLAVDLIEFNPRQPR
                     ENFSAESLQELANSIKKCGILQPLIVAKTAVGYELIAGERRLKAAKLAGLKQVPVIIR
                     SADEEEKLILALIENIQRENLNPLEEARAYRQLLDEFHLTQDELAKSLGKGRSTISNI
                     LRLLNLPSKVKEALRAGQISVGQAKVLAGLEPSKQIEAFSRMASKNKRITVAETTKSV
                     RHLQGLASSSDNIPKNNDRGREFLLQEFLQAKVKIVREEHGGEIRIKFNNDDDLLRLM
                     DRIRHS"
     gene            complement(44027..44785)
                     /locus_tag="PK960_00315"
     CDS             complement(44027..44785)
                     /locus_tag="PK960_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPD07655.1"
                     /translation="MSKIIAIVNQKGGVGKTTTAVNLAAYLAKMGRRVLLIDIDPQGN
                     ATSGSGFLPAELSSGIYEALIGQRSMADIIVNSPNGYLLAPSTINLAGASVELVNLEE
                     REFKLAQALDSVQADFDYIIIDGPPSLGLLTINSLVAATHLLIPVQAEYYALEGLKQL
                     LDTIGLIQAHLKPNLGILGAVVTMYDQRNRLARAVLKEVEQFFPNHVFKTIIPRNVRL
                     AEAPSYGRSIVDYDKHSTGSRAYEALAKEILALK"
     gene            44866..44949
                     /locus_tag="PK960_00320"
     tRNA            44866..44949
                     /locus_tag="PK960_00320"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44900..44902,aa:Leu,seq:caa)
     gene            44984..45805
                     /locus_tag="PK960_00325"
     CDS             44984..45805
                     /locus_tag="PK960_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07656.1"
                     /translation="MEETKYFYPIITFTNWLAPILEWCSLHHYQPQDPNRLARIIINS
                     YLVAENETAPSNIEKITGLIKFAESFITKEKLLLCLGEIGFSTQYPEVVWEIKKSNNW
                     ADCLAAEAKLLERLSPTTPGAWLMSKDELNQFWVWWQWIADQTWHWVKTPDFPLSHFL
                     IQEHLSWAQNIITSFRRNYCLLSDSLEENETNIRNWLQRVAPNADNITIELAIKKLMG
                     LRAPLRAGWDKALQTAMKETADQQSFWRKNLDPTPFAQMPDGLSLIGESLQQIIT"
     gene            complement(45824..46501)
                     /gene="trmD"
                     /locus_tag="PK960_00330"
     CDS             complement(45824..46501)
                     /gene="trmD"
                     /locus_tag="PK960_00330"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPD07657.1"
                     /translation="MLFQVISVLPEAFSYLDQSILGRAQEQGYFQVKIHDLHNWSLNK
                     QRRVDDTPYGGGPGMVMRVEPIARALEDIKTNYAKRKVILLSPRGNKWTQAKARRYSQ
                     LDEVVLICGRYEGVDERVAYLVDEQISIGDYILTGGELAAMVIIDSIARLLPGVVGNV
                     ASLLEESHSHVGQLEYPQYTKPEVLTLNGRDYAVPDILLSGNHQAIAQWRRAQMRLGK
                     NKKPLAK"
     gene            complement(46587..46922)
                     /locus_tag="PK960_00335"
     CDS             complement(46587..46922)
                     /locus_tag="PK960_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPD07658.1"
                     /translation="MELKDKEFLEYVVKAIVSQPEDVVVERTVDEMGVLLTLKINPAD
                     MGYVIGRRGQTAQAIRTLLKIVGAKNNARVNLKIYDPNAGERPRREPRPQRPTEEKID
                     TSSVENLSI"
     gene            complement(47016..47441)
                     /locus_tag="PK960_00340"
     CDS             complement(47016..47441)
                     /locus_tag="PK960_00340"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPD07659.1"
                     /translation="MQSSRPLSRLYHFFLSQFKSPQFLRYFIVGCSGVVLDLTTLYLL
                     KTIVGLTPVVAVIINQIGMGAYIFLLNKYWVFGSSGTFTRQATRYIILTIVNYGIAIF
                     WMWFFNGYLGFNYLLMRLANIALSTVWNFLLYRFFVYRK"
     gene            complement(47450..49411)
                     /locus_tag="PK960_00345"
     CDS             complement(47450..49411)
                     /locus_tag="PK960_00345"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPD07660.1"
                     /translation="MKILYSQIKQLIPSLAATPAEIGEALTMTGFMQESLEMVNYNGQ
                     PDVLLGFEIRQNRPDCLSVIGLTREIAAYFNLPFNLPPTTELTPAETEVPIRIETSAV
                     RRLKAVKLQNISIKPSPQWLVDYLALYEINSINLPVDLSNYVMLLTGYPSHLMDYELL
                     DGDTVWSINHQSREITTLDGTSVKLKDEELIIHDRHSIIALAGIIGTDYAPITEKTKI
                     ILAEMALYDPALIRRNVRALHVVTEASNRLSKNLSLVDLDYAFNLLINLLLDQTGGEI
                     ASQVVEDYKEKTEVKTIKFEPQLVSELGGINIELAQCRQLLSQLEFTVNDAQLPWEVT
                     VPSYRTDIELPEDVVEEVLRLYRFDKLPSVLPALPATLDITPPLTKAKERWRDWLAFQ
                     GWDEVLSWTMVGLEDNALTNYLPWAIATVQNAINEDYPALRQTIIVSLIKQAHEYAKE
                     QVPELKIFELGKVFGKVENSYQEAEHLGWLVGGGDRVIDDLRQTVSGFLAYSGVERVY
                     FYPAKNIPAIANPYAAWEIIIGEEPIGFLAQLKEHYNDKIMATAEIDLEQLISYLQEH
                     KMPAPTVEITQKLITLDANLEADDRRELGIKLQDIEDKLGRESLWSLAVIDEFSLPNG
                     RCRYTIRAVYKELDDQSAKKLHLQVFGLS"
     gene            complement(49395..50420)
                     /locus_tag="PK960_00350"
     CDS             complement(49395..50420)
                     /locus_tag="PK960_00350"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPD07661.1"
                     /translation="MLGDQILTELSTQIKEVGSEAELAALWHKYLGREGIIKAQVKAL
                     AQLPLEEKREQGPLWQRIQEEAQALFDTRAAELKKASEEATWQAEAQSLGLVPVKLGH
                     LHPITQTIRKLNELFISLGYSIMEEREIETDEFCFQRLNVPLDHPARDMQDTFYLEEP
                     NILLRTQTSSVEARVLANYAPPLKVVCPGRVYRNETVNKSNHFIFHHYQGVVVMPKIS
                     LSDLFGTFTALFQAIYGPEVKIRFRNKYYPEVEPGVGPDMQCFNCGGKGCSVCKGVGW
                     IEMGGAGLIHPKVLTMAGLDPREWHGFAFGLGLDRWAMASYNITDIRTLLGGNLGYKP
                     YHHENII"
     gene            complement(50424..52127)
                     /gene="argS"
                     /locus_tag="PK960_00355"
     CDS             complement(50424..52127)
                     /gene="argS"
                     /locus_tag="PK960_00355"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPD07662.1"
                     /translation="MGWYQEITKEITELINHELPEPLASPADLVIPPQRELGDLGWPC
                     FAVAKKLGYLPGELAEKLVTQLSTKEHPAIGGWQAVGPYLNVTLRGEVLINKVIGEIE
                     SAGSQYGSINSGAGRKIMIEYSNVNTHKDYHIGHLRNLCLGDSLTRLFNFSGYKVIPV
                     SYLNDLGIHVAKVLWKLNQIPENELPHKNLGHYLGELYTQAAQELANDPVGQELVSLI
                     LRRLEAREQPEYSRWQETRQWSLDYFQKIYEELDIHFDQTFFESDFIDEGRKLVQEML
                     AKGILEQNEGAILANLESEGLGVLLFLRRDGTTLYPVADLPLAQAKFRDYDIDESWYV
                     IDERQSLYFKQLAAILAKLGFNKPIRHLSYGFVKLPTGLMSSREGNMVTYEALREALL
                     TQELREVKERHPEWSKEQIQETAWQVVVGALKFEMVKVSPARPIVFDIPQALSFDGYT
                     AAYIQYAAVRLASLERQAKLKGNGSSTLIARPNELEKALALTLTRFSEIVSQASQAMD
                     PAIIAKYLFDLAQEFNNYYQKVRIIGSSEEMGRLRLGAAIRQVIINGLALLGIKAPEV
                     M"
     gene            complement(52131..53330)
                     /gene="tyrS"
                     /locus_tag="PK960_00360"
     CDS             complement(52131..53330)
                     /gene="tyrS"
                     /locus_tag="PK960_00360"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPD07663.1"
                     /translation="MVNTNPKLIDELLTRGVNEVIVKEKLREKLLSGQTLRVKLGIDP
                     TSTKIHLGRSVPLLKLRDFQELGHQAIFIVGDFTGVIGDTSDKESERPMLTLEQVEKN
                     MKEYFNQAGKILDLERCEFHYNSEWLDKLGYREIATQADQFSVAEFIARDNIRRRLEA
                     GSRVSLREVLYPLMQGYDSVAVRSQVELGGTDQRFNLLAGRTLQTYYGQEPQDIIMTE
                     LIEGTDGRKMSSSWGNTINLVDDPQTMYAKAMGLQDSLIIKYFLLITRVPLKEVKEYE
                     VALKKGVNPRDIKMVLAKTIVTMYHGVAAATTAENYFITVVQQKNLPEDIPLIALAST
                     TWPLIDLLVHLKAVPSRSEARRLIEQGGIKVNGEVITDSTATVELSSEPLLLQKGKKQ
                     FWRLVVS"
     gene            complement(53335..54102)
                     /locus_tag="PK960_00365"
     CDS             complement(53335..54102)
                     /locus_tag="PK960_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPD07664.1"
                     /translation="MTTLLNILISFFVLLICWLLSQVVTNYSEQLDKRHRTAKFNPTM
                     RLIIRRSGQIVIYLIGLLIIGKIWRIDIGPLWAGLGVAGVAIALGLQETLSNLFAALF
                     VLLDKTIQVGAYLRLDDGTVAKVEDISWRSTRLKTASGEKIIMPNRLFTNQRVITYDE
                     ADRGWVLTLSAVAGPGSALKEVKAGALAAGLKVAKKYQKKVSPPQVYFEELTPAGLKF
                     KIIIKIDKVTDEPLARHELLENLIMELNKRSCPLVGR"
     gene            complement(54119..56755)
                     /locus_tag="PK960_00370"
     CDS             complement(54119..56755)
                     /locus_tag="PK960_00370"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPD07665.1"
                     /translation="MTELAPTYDAKKYEAAIYQRWLASGFFNPDNLPLPPEAPNYTIV
                     LPPPNITDKLHLGHAAMLAIEDALIRYHRAKGYRTLWLPGTDHAAIATQNVVEKMLYQ
                     QEGKTRYDYGRTEFLRRVWEVVLPIQTTILDQIKKMGASLDWSRLAFTMDEPRQKAVR
                     HMFVEMYKEGVIYRGERVVNWCPRCQSTLADDEVDYREQMATLYTFKYSPDFPIPIST
                     TRPETKLGDTAVAVHPQDERYQEYIGKTYSLDFCGQPLTIKIIADRSVDRNFGTGAVG
                     VTPAHSLTDWQLAEKYNLPMVKVIDTDGLLGDNLGQFSGLSTLAARDLIVAELKKRGL
                     LIKEETITNNLSVCYRCGTTIEPLPSKQWFVSVDKPITRLGGRSLKTAAYEVAKTGQM
                     KFIPERFTKRYLEWMDNLHDWCISRQIWFGHQIPVWYQGDEILVSETAPTSPGWIQDP
                     DTLDTWFSSGMWTFSTLGWPDNWQAGHKLGDLARFHPTEVLETGYDILTLWVSRMIMM
                     SLFAVGEIPFYEVYLHGLVLDEHGQKMSKSKGNGLDPVAMIELYGADAVRLSLLIGVT
                     PGNDCRLGTTKIESCRNLVNKLWNIGRYILSIIPDKAIFVHNNLDLSQTTLADRWILR
                     QFNDLISAVSNYLEHYEFSLAAEALRDFTWHDLADWYIEISKIEDSKEKCNILAYILK
                     HLLILWQPFMPFVTQVLWDELGVEDNLLIASWPQVLKINPEAEQEELDFKLVQDLIGE
                     IRNIRSLNHLQPSQKMKMTIIAGQWADALSKEIKLIQNLRTGLETVTIIANGQVPEGS
                     LAGAVGPFSFYLEGEAMSAVNHAQLEHKIQELTTAINNLSRRLNDPEFLSKAPEHIIA
                     KEQTKLTNWQAELEALRQQLNS"
     gene            complement(56866..58356)
                     /locus_tag="PK960_00375"
     CDS             complement(56866..58356)
                     /locus_tag="PK960_00375"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPD07666.1"
                     /translation="MSLGGGQSSRSSYPWLAFIIGVAIAGLWPEQWPAIYYYVGLVGL
                     AWGSSFIPRKTRWLIILCLALGFGRAHWALVNKEAATHQLICSNKIVTIRGEIVGQPQ
                     PRSSGGSQLIVKIKSAQCGVETVSVSGRAMIYGPDSWLDSGVGEEWQFTGKLKNWPEG
                     GVFSYRYSLFSRGVGIIMASEKRQLVGRQPLNLWLKLVNGQQRLADVLTTSLPEPAAS
                     LAGPLIWGGAGRLPAEWRNALALTGLSHITAVSGFNISLLIILLFNNLLLLHCPRLLA
                     STTTSLVIVLYLALIGAPASAVRAGLLGLLMLWGRQLGRLGQAYHLLLISASLMLLAN
                     PFYLWGDLGFGLSYLAVLGLMTWTKWWTKLIERLPLIRYPLIRDNLAVTLAAQTWTWP
                     LIFWQFGQISVIAPLANLLVVWALPWLTIFLLLASPLGWLWPEAQVFIFMPAGWLLTI
                     LLTIIKYLAHLPCAGFIVNWPDNWRIIICLVYYILTLILLKNWKFS"
     gene            complement(58407..59456)
                     /gene="prfB"
                     /locus_tag="PK960_00380"
     CDS             complement(58407..59456)
                     /gene="prfB"
                     /locus_tag="PK960_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPD07667.1"
                     /translation="MGDVFDLTSKANELTKWRQATMASDFWSQPTKAGELLQQIEATE
                     QELKSYNQLLQRLDDLTDLISQENSISEDINQLVQAEYHQIAREIDQLEISRFFTQPH
                     DRLPAILSIHAGTGGVEAQDWAAMLERMYLRYSLRQNWTSQIIEEQRGNEAGIKSATL
                     RFVGQWAYGYLKGENGTHRLLRNSPFNAQHLRQTSFAMVEVYPELPEAEAPAINTNDL
                     RIDVFHSSGPGGQGVNTTDSAVRITHLPTGLVVTCQSERSQHQNKALALAVLRSKLAN
                     KEAEELEKKRQVLKGGQVKADWGLQIRSYFLYGKRLIKDHRTNLTTTDIDAVLDGHLD
                     DFIRAYLLWELERNK"
     gene            complement(59529..59747)
                     /locus_tag="PK960_00385"
     CDS             complement(59529..59747)
                     /locus_tag="PK960_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07668.1"
                     /translation="MDEDWRRLLAEQTADIAQIKKEVHRVYKYIRGQRLLGYIKIAII
                     ILTIVAGVVYLPPLIRNLVSQLSPLISK"
     gene            complement(59801..60508)
                     /locus_tag="PK960_00390"
     CDS             complement(59801..60508)
                     /locus_tag="PK960_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPD07669.1"
                     /translation="MIIGGFEPLSLLDYPDKIAAIVFTQGCNFRCRYCYNPSLINLRG
                     GGGYDLSKFWSFLAERQGLVEAIVITGGEPTLQKDLLKFIQKIKEQGFLVKLDTNGTN
                     PQIVKDLIAQGLLDYLAVDVKAPWSKYSQIMAMPEAVLTVLVAKVKELVTWLIDKTPI
                     SYEFRTTLVPGLLGLQDIAELAEELKGAQRWYLQRGRLDGNLLDSTLAGQSPFTEVEM
                     NNFLAVAQTKVAGVAVR"
     gene            complement(60510..62739)
                     /locus_tag="PK960_00395"
                     /pseudo
     CDS             complement(60510..62739)
                     /locus_tag="PK960_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256225.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
     gene            complement(62744..63199)
                     /gene="nrdR"
                     /locus_tag="PK960_00400"
     CDS             complement(62744..63199)
                     /gene="nrdR"
                     /locus_tag="PK960_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPD07670.1"
                     /translation="MKCPICDYSETKVIDSRVVSDGLSIRRRRECLKCGFRFSTYEEM
                     EILDLTVVKRDGRKELYNRDKLISGLRKATEKRPINDDQFKRLVNLIERDIQVRRQGE
                     IDSRDIGRIVMRQLKKVDKVAYIRFASVYSDFELQDFSEELNKLLKLTK"
     gene            complement(63381..64583)
                     /gene="ftsZ"
                     /locus_tag="PK960_00405"
     CDS             complement(63381..64583)
                     /gene="ftsZ"
                     /locus_tag="PK960_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007086277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPD07671.1"
                     /translation="MTEVKPAVEAPANIKVVGVGGSGGNAINRMIEGGIKGVEFIAIN
                     TDVQDLNYNKSPLKIHIGKTITKGLGAGMNPDLGRDAAAESQNEIRDALKGADMVFIT
                     CGLGGGTGTGASPIIAELARDLGALTIAVVTKPFTFEGAQRRKIAERGQNELIGKVDA
                     IITIPNDRIFQVIDKKTTILEAFSIVDDVLRQGVQGIAEIINTPALINVDFADVKSIM
                     VNAGSTLMGIGQASGENRAIEAAKAAINSPLLEMSIDGAKGVLFTVTGGPTLALDEIH
                     EAAKIITNAVDEDARIIFGTTVDNNFKDEVRVTVIAAGFDNPVSTEGEAISRAFDSYY
                     KPTSFVGKNQPEGETEVKEGHKGFMSALKKAQAEPAKPAASETPEPSEGEEDDDLSIP
                     AFLRKKIM"
     gene            complement(64657..65913)
                     /gene="ftsA"
                     /locus_tag="PK960_00410"
     CDS             complement(64657..65913)
                     /gene="ftsA"
                     /locus_tag="PK960_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPD07672.1"
                     /translation="MRDQSLIAGLDIGSTETRLVVGQVTAGQLQIIGAVAAPTAGMSK
                     GIVNNIEEVTSSVLACLEKSERLIGVHLDSVYASVSAPSLKCERSKGVVAINQANNEI
                     TVEDMNRVIEAAQALSLPANYEVLHVIPINYRVDNQEDVKNPVGMSGIRLEVEVLIVQ
                     SLSNQVRNLSKAIKQAGLRIDNIIIAPFAAAEAAVTPRSKELGVVLVNIGSLTTSLAV
                     FEEGELLHAAVLPIGSDHITADIAIGLRCSINLAEKIKLEYGQANPDKIDKDEELDVT
                     SLITPDNEVDEPMTVSMKYLSSIIEARVGEIFTKVDAELKKIGRSGMLPAGVVLVGGG
                     AKLAGIVDLAKKNLRLPVCLGANRNVPSVVDKVNDLSYLTALGLVAWGDHYSSVEEED
                     NSGHGFKPGHAVKRVKDWFRSLFLKD"
     gene            complement(65918..66295)
                     /locus_tag="PK960_00415"
     CDS             complement(65918..66295)
                     /locus_tag="PK960_00415"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HPD07673.1"
                     /translation="MLSGNRLWRSFRYACRGLWRVIGEEQNFALELAIAVGVVLLAVL
                     SRFKAVEWAILILTIGLVLLMEVFNSAVERAADLLKPRLDIYVKEIKDIMAGAVLLVA
                     ATAVVVGVFLFGPRVIKLFTTII"
     gene            complement(66289..66657)
                     /gene="ybeY"
                     /locus_tag="PK960_00420"
     CDS             complement(66289..66657)
                     /gene="ybeY"
                     /locus_tag="PK960_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPD07674.1"
                     /translation="MVKWQKLSQLFLDEYGVKGEVSLVLVGDRRIRNLNKKYRRQDRI
                     TDVLAFADGENGQLGEVIIDYPQIVRQAQRFGHSVWQELIFITTHGLLHLLGYEDDSE
                     EGRQTMIKIGEQFLNRHNIC"
     gene            complement(66699..68063)
                     /locus_tag="PK960_00425"
     CDS             complement(66699..68063)
                     /locus_tag="PK960_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07675.1"
                     /translation="MSEMPTNNFKITYSDDLNPPADQIEFVNQNETTPGSEEGQYIYT
                     MPSAFRFGHRTSFTNKAGLLIMVVGLLLLVGLVYVGFLIITKNPIFNFNPAPTNELVA
                     VTSSMPTTTLTESSPTTSSEVIIDLSDPEQAYLQLRLQLDQALTLEDYLKAFIAGASR
                     AKAQELTEQAAEINNLSESQKSSTLSISKSMLIPLEASDKITKEINGNQATLTITKAD
                     TGRVAIVTMLFEDGQWRLDNEHWQDEVTTSTTEVVAPEVSESSTATTTKELLIEDLNL
                     DKDSDQDGLTDKEEALLGTKSDTADSDGDGYDDLTEIRSGYDPAGQGKLTNNRNLKLV
                     EHGPWSVIVPASWTQQLGRDNSAIFRADDGQFIQLTILGKVASQDLASWYQEMFNVDA
                     DQVPEITTHGANEVVMSPDGLTYYLTKPGLDDLIALTYNPESNYLLSYNNIFHLMIDN
                     LIIK"
     gene            complement(68090..68881)
                     /locus_tag="PK960_00430"
     CDS             complement(68090..68881)
                     /locus_tag="PK960_00430"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07676.1"
                     /translation="MFEEVNGQKSSTPGAASENKDNKETEAIKNQPETGAPQGETTVQ
                     DIFANVDKASPKAPGDSQAFGTINNVPEMASNNQGTGQISAKPRSKVLPIVIILVVLV
                     IIAAGALIYVQKSRQATVTVDIPSSIVNVEEADVDTSTPASNPENGEELMEIATSEPV
                     IINELTEEAVSTDEASLETDGSNNSEEVVPEEPEISETVETEESLPSDEVVNLDSDQD
                     GLSDADELNVYGTNPLKSDTDGDGYLDGEEVKNGYNPLGEGRLSQ"
     gene            complement(68944..74679)
                     /locus_tag="PK960_00435"
     CDS             complement(68944..74679)
                     /locus_tag="PK960_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPD07677.1"
                     /translation="MSWGKKIFLSGLIVVSLASFGHLSVAQAQGLTSEDLGLQYASST
                     GLSTQDPRITVAKIIRIVLGFLGTIAVILIIYAGFVWMTSNGNEDKITKAKNILKGAV
                     IGLVIVLSAFAITTFIINSLLEATGGGGSAPPSPPGPPGPPEPSGDLIITGVSPADGA
                     SGVPRNTVIRFQFNGSIDPDSVTNASAAVQADDQPLTGQWTVNGREIAFQPASICSSS
                     CGEVNCLPEDSHITVHLTSGIKSLGNNPKNLVCSGLGFSCDLSFSVGSLVDCQDPQAE
                     FLSTIGLCAATNNLVKVMASDDTGLARFEFLANGQQFYLSTNDFGLNYTAIADWQPTS
                     TAGEPFTLEALVDDIAGRRTTIEQRGRLLPAHCCNGQQDADESGLDCGGSCLKCEGAA
                     CANDQQSPGTSCNSNLCYSAFCTTQGSSAASCEAAGYPKGTASCCLCRNRPRITDLSP
                     QGGFCRNNYNQYCNTDSECANGDTCDRNSPNAKVGNLITIFGTGFGSSTGSVIFANDK
                     LGDLNVCGNTAWSNNQIIVKVPSGATTGIVQVKNSDNFIATSSQELIINNISRPGLCS
                     LDKASGTTGEIINYRGLNFIQTGATSTVALYGNLSNPIPANESEVMASNLLKAWVPSL
                     QPGWVSTFVRNDNPILPGPASNFLLFNKLVDPGSGPQITGFEPMSGPPGQYVTIYGHG
                     FGNIRGNSHVFFGSTEADYNFPEICATAVWSDRQIIVKVPSGANLEGKQLLSIQLGTG
                     DILEASKLFIVNSDPLAPSLCAIRPTTGQVGTQVEVWGEHFRNYDPMTSRFQFSPNII
                     ATSVSWFDDGTVSGASSTVPAQAISGIVRVKNIQGVSNGLNFMVGACKTNDNCPGQIC
                     CPAPSADAGQCRDNLAACYGSASSCVYRWSFTTGSGNVTCPPNTTICGSKCCSADQQC
                     VNNVCTNKTILSCNDYHQCNGALYCPNSPGWCSQKPGSTIDGPCGDSYCAQLFGEQTS
                     GGGHSTGVAPLQITYNSTTNRCISNVLCDLDKKVNFKLGDNTVNYALTCQGKHWTTHL
                     TAGQCPPTTSEYGQWQLLANNICRSNMACLNCETPLQCLAVGAQGRCGLDEPICASGF
                     QCSNNQCRKEAGLCECCCDENLNRPDQTNPGCCLPLTCNGTCGSEERFGACSGCAAVG
                     PTTSTRDMACNCLGTTGKYCNTSVANGICSDCAQLGKEDCLAHTTCCYDQATSQCRGT
                     VEGNRLVNGYCGYYVCNNNKCIAELQSEGQFTTLAECQASCQVVNLQGESCNVATTTF
                     LWPNYINHVGCGYQLCASPFNCRTASGLSSTSTNQADCGICCCDPAHDQCSQVSPNLY
                     CDANRDQCSGANRGLCCGCERDEDCGSIIYDGCGQDSCCRARPYIVKTVPDNEAQQVC
                     RNSLIDVEFSKTMAATHLEDNIFLIADYGQKDPCPTGTTYLAAADLGQGGWWVKLKNR
                     LLTWFRPDVAAEEGILPDNTYCALPKSIELSGKHLTIHPANVLPANRRIYVVMRADNN
                     LQDSQPEGILDQNGIGLANGKPLQFGNLELPAALYWSFKTSDQVCRVDHVELNPTSIL
                     FSDLQASSTIEATAVDLNGQILVPYPSYNWQWLWSIGNPAVAQLVSYNGNRALLRPQN
                     KKDAETYVEAIAQVTADQTAVPGALLPSFSKTALIRVFLCANPWPARRPDGSWQPWTD
                     SVTNMQFYYCRDREGSSQAGDLPALKVVTSTDPGAMVPAFYFFHYSSVTSSPTNLVAT
                     PLPAGGKVGLRWDAYPNASGYKVYYGLNSGQYFVDPIITTSTQVTIDNLINGQLYYFA
                     VSALIHLDAAAFAETPFSAEVQARPQDIVPPSAVHCFERTDGEHQINLVWNRNQDDTV
                     RYRVEYRIAASPSESSQILLVSQPASGNKVRTSITNLINPKRVIVNVYAVDAAGNVSP
                     AQLPYGSECNK"
     gene            complement(74687..77248)
                     /locus_tag="PK960_00440"
     CDS             complement(74687..77248)
                     /locus_tag="PK960_00440"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07678.1"
                     /translation="MTTVLRRFAGLGVILFMLTLAVGPSFPAMAQENGDNLGLEYMAE
                     TGLSREDPRIVIARIVQIALGFLGTIAVVLVIYAGFVWMTSNGNAEKIKKAQDILKSA
                     GIGLLIILLSFSIVTFIIDKLIGAGGGGGGGGGGGGGGGVTTSTFGALGNGIVESVYP
                     APGQTDVPRNTSVIVTFREKMKVSTICDGGNDATGICVNTNIKANNIRFFVTEVGDNP
                     ATNLAGDEVKVSTNDGKTFVFVPVNYLGSASGNVWQSVKLTTGLLKANNEPAFTLSDF
                     TWQFEVSSRVDLTPPQVRLGGILPFPDNGRDIHGNTQLARVATASIEVVSLPLAMASA
                     VTGPAESQGNAGAARVEGTYNCSDSGTITVTVVPGSPLSAQAIGISGLINDDDVSDGH
                     ISLGCGLTLIKNGSLPPDNHGRLSPGESWKIAVTAAQAGETLTVGSQTYRFVSSADSN
                     ADHIVVAATIQGTADNIANKINGTNIDVSAQSDGNIVYLQARRAGTSGNFLSLSTDSS
                     HLSLTPFSGGQDREENIQIVDRPDAPRNSIIQINFSEPINPLTVVGDSNYVSSTIEVL
                     TTSSQPISGRFVISNQYQTVEFIPDTECGVNACGDKIYCLPASSTIVVKLKAAALASC
                     TEDKDCISKTPYNQCFNGSCRRAEGDHYPLANLPLTGGIVDVCFNSLDGNRNSKVVGP
                     VNFYNENDQDSAHGDSYRWSFFVSDEIDLTPPRLQADSLIPALHSTQGLLNQPIEFSF
                     DKLMMASTLLSGETIINDMHHHLINLFSAGLAPSYWISSEAVDAGIPDGYPDFTKVII
                     NHGLLSDATKYFIEIGSGVKDIRQNCFKPCRDDSTCGDAIGSCCEGLPSALPLCN"
     gene            77328..78848
                     /locus_tag="PK960_00445"
     CDS             77328..78848
                     /locus_tag="PK960_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HPD07679.1"
                     /translation="MTIEQVIAQTQGHLSKADTELIKRAYNFAAKAHRGQKRITGEPY
                     IEHPLQTAFILAQMGLDAKTIAAGLLHDVPEDSKYTLSDIKKNFGEDIAFLVDSVTKL
                     NLIEYHGRTRYRESLKKMFLAMAKDVRVIFIKFADRLHNLRTLEYLPEDKRQKIANET
                     MDIYVPIAGLLGIWHLKWQMEDLCFKYLHPTEYQELVQKYEVEKKLELNQYIEQMATT
                     LSQVLTQLNIEHKITGRFKHLYSIWEKMHDKNRKFDEIYDVFALRVIVPDVDNCYRTL
                     GAIHSLWRPNINRFKDYIALPKPNEYQSLHTTVFGPGGKATEFQIRTPEMHEQAQYGI
                     AAHWSYKEYGSKRPTPKVGWIQEILNAQKNSPDTQTFIQQLRLDIFSDRLFVLSPKGD
                     VYELPVGATPIDFAYAVHTDVGNKAKGVLINNKISRLDEPLHNNDLVEIIIDKQQTGP
                     DPAWLKFVKTTKAKNAIRQYLKPSALNRFAALANPKHWWPRHHNPNQPPEKNKNLE"
     gene            complement(78864..79091)
                     /gene="rpsU"
                     /locus_tag="PK960_00450"
     CDS             complement(78864..79091)
                     /gene="rpsU"
                     /locus_tag="PK960_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPD07680.1"
                     /translation="MVEFKRKPGESFESFIRRFNKRLQLDGKLMLAREKHYYYKKPNK
                     RQAKQSALMRQALREKREYLRKIGQIKDDFH"
     gene            complement(79113..80447)
                     /gene="hisS"
                     /locus_tag="PK960_00455"
     CDS             complement(79113..80447)
                     /gene="hisS"
                     /locus_tag="PK960_00455"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPD07681.1"
                     /translation="MTKKHDKLVDLVTPSRPTGPIAHRVKGMKDIQPGAYPAWEFLLQ
                     SAGRLARLRGFERIDTPVLESYSLYERSSGRTSDIVTKEMYSFLDKSGERLALRPEAT
                     PGIVRAYIEHGWFNLPQPVKTLWYGPLFRRDKPQLGRYRQHYQLNFDIFGEPGPLAEV
                     ELIFLIHELLNDWGIEAQIDINSLGCVDCRPNYINELVKYYKEKKHREQLCPDCRKRL
                     IKNPLRLLDCKEPACEALAQGAPQLIDFLCEPCKQHLVKVLEFLDNLGIQYNLNSQLV
                     RGLDYYNRTVFEVVMPSESAQRPLALGGGGRYDQLIESLGGRPTPACGFALGMERLLL
                     YLDPNKVEVSQTRPDVFLAQLGDGPRLKSLQLFEELRQAGLTVHQAFTKNSLKSQLAL
                     ANQLKVKYALIIGQKELLDGTILLRDMESGSQEVLDLKRVKSIMLKRLAKTK"
     gene            complement(80466..81050)
                     /gene="lepB"
                     /locus_tag="PK960_00460"
     CDS             complement(80466..81050)
                     /gene="lepB"
                     /locus_tag="PK960_00460"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPD07682.1"
                     /translation="MIFTPDFKVRWRARWLSFLEIVKIVIISAAIVIPIRLFIVCPFY
                     VQGASMEPNFYDKEYLLVDEISYRFREPQRGDVVVFRYPEDPREYFIKRIIGLPGETL
                     KIENGGVYVLDKSNNSWTKLSESYLSSSNETSAFETKQITVGPDEFFVLGDNREHSRD
                     SRYFGPVNRRYLIGRVIFRGFPLKRLQFFKAPTY"
     gene            81214..82953
                     /locus_tag="PK960_00465"
     CDS             81214..82953
                     /locus_tag="PK960_00465"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPD07683.1"
                     /translation="MNQPFNAPSDDLLDEMLNSPENNPEANSEPLEPADNLSEPINIT
                     IEAKQSHQPRQEPRENTPSLHHPAIIEKPRPRKKHHLGYFFLIIGLILLGYLGWRVWS
                     ATPVNLETNNSSGNKQELSFWKSLSLLTKFKLGIHERVPLKGEAEDRINFILLGMGGP
                     NHDGAYLTDTIIVGSFKPSTKRLVLMSIPRDLSVPIAGYGWRKINSANALAEAKEPGS
                     GGQATADLIGEILEIPIHYYFRVDFAGFVKIIDDLGGIEVDVDNTLDDYSYPIMGEED
                     NPDYYKRFEHLHIDAGHQTMDGELALKYARSRHAVGVEGSDFARSKRQQKILMAIREK
                     LFAASNLFKPKLIMNIVSDAENHISTNLTPEELVSAWQLAKDVKPENITTINLDNSPT
                     GLLYDQKGADGAYLLVPRTGDFREIIARFNEALLGSTTTATINNEAVSSTSDISSQPT
                     AESLTKQNNTINPADLPTKANAKLDIRNSTNINGLAARTKTKLQKYGFTVVNVSNTSH
                     KNLPTTTIYDLTYGAKPDDLALLQYQTKAALADDIPNWLAQETSELIKEQPDMTQPDF
                     ILILGQDYSGKNQ"
     gene            82955..84373
                     /gene="der"
                     /locus_tag="PK960_00470"
     CDS             82955..84373
                     /gene="der"
                     /locus_tag="PK960_00470"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPD07684.1"
                     /translation="MPTSKPQPVIIFGRTNVGKSTLFNRLGDTHQALVSSTSHTTRDA
                     NHKIINWNHQYLELIDTAGLSELSYLTMSPKKAARLTELQAKMQYQTGELLRTAAVIL
                     FVVDARVGLLPGDKELARACQKIVSPNTPIILVANKVDNSRYLSEMTVFYTLGWSDPW
                     PVSALTGMGTGDLLDEVVRRVKKQGSRSPQKPPLDYNSTAKPDYRVCIIGKTNVGKSS
                     LFNSLVGTERMLVSTQPHTTREPQSAILKYQEKTIEIIDTAGLSRRQPKAKNNLEKRS
                     MIKSLACLKDADIALLTLDISEPITHEEARLAQEIIDHKKSLIIVANKWDKITNHDPK
                     EFSALVYDRLPFIKFAPLVFVSAKTGAKVQKILDLILEVGQDRAITLSPSQLNHRLAQ
                     IVKHQPPTQGRGLKHPHIYTLSQVKTNPPIFTVTLGPKDNLQPAYLRYIQNEIRKSYG
                     FLGTPITIRINHRRSSKPPELS"
     gene            84378..84929
                     /gene="pth"
                     /locus_tag="PK960_00475"
     CDS             84378..84929
                     /gene="pth"
                     /locus_tag="PK960_00475"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPD07685.1"
                     /translation="MEKIIIGLGNPGQKYHHTRHNAGWLVLDRLVQELLDEPPHWENK
                     PRLFAQIIVIGHNLLVKPTTMMNDSGRSVRAIVDYYNLTPADFSTKLLVIHDDLDLPL
                     GQTRWSKNSRSAGHKGVQSIIDALGTQTFNRWRLGIKPASPLLEEPATFVLNNFSPLE
                     LQILQALSLKPIYNFLEDQPSNN"
     gene            complement(84919..85908)
                     /gene="trpS"
                     /locus_tag="PK960_00480"
     CDS             complement(84919..85908)
                     /gene="trpS"
                     /locus_tag="PK960_00480"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPD07686.1"
                     /translation="MSTILTGDRPTGRLHIGHLFGSLLTRVKLQAEYDTYVEVADVQA
                     LTDHFKQPEKIKDNVYEVVLDNLAAGLDPHRAIIFIQSLVPEIAELTVFFGNLVTLSR
                     LERNPTVKSELEQKKSLFKGGVTYGFLGYPISQAADILAFKAEAVPVGADQEPMLEQA
                     REIAHKFNAIYGKTFPEPRAIFSDFPRLKGLDGSVKMGKSLDNAIYLADEPAVIEKKV
                     KTAVTDPQKVRRHDVGHPLICPVYSYQQIFNASQAKTIAHDCQTGKLGCVECKACLAQ
                     QIISYLAPLQERRAHYAQDMPKVRDIVRDGTAKARQRTRETMEEVREKMKINY"
     gene            complement(85938..86993)
                     /locus_tag="PK960_00485"
     CDS             complement(85938..86993)
                     /locus_tag="PK960_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPD07687.1"
                     /translation="MPAKVNNPIHINITTSTILKVVGILFLLYVAFLIKEVLSVIFVA
                     LVLASAFDPLVDYLGRHKWPRALSISLIYLVVLGLVVLMFYFLVPVVIKEATDLANSL
                     PYYFGKLSSFLGRVRETMGQYGLAASFQTNLDSLASTISGWTMQLITAAYGAVNGLFS
                     LFLILVLSFYMVVEENAIKKMVWAVTPARSQAYAVNLVTRMQLQIGYWFRGQLLLCLA
                     IFLLTLIGLSILGVRYPLLLALVAGITEVVPYLGPVIGGIPAVLIALTQSPRLAIVVI
                     IVYVIIQQLENNVLVPQIMKRVADVNPIASIIVLMIGYRLAGVIGAAIAIPITTAFTV
                     ALRDLLNYKEKKELQPT"
     gene            complement(87034..>87711)
                     /locus_tag="PK960_00490"
     CDS             complement(87034..>87711)
                     /locus_tag="PK960_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07688.1"
                     /translation="AKITINTGAKLVQAYLYVPDNDNDRHSLPWALGQQIYSVGTSTP
                     SGYNSRNIIIGLDDCCDNNSAAVPGAAVYHSSTFTNQVITSSPLFGFDWDCNGNVEVR
                     STQSKFCQSVDPIPVGYIYRHQYCDIEYEKGRGCCTDDGGGGGGISYRDGKQLLSLIS
                     LAQAMPNECCEPWGSFDGCSYVYNVPTSACGTSKYIAWSSGYADNRNCVSYVPYYYAQ
                     NMPVTCK"
BASE COUNT        27659 a        20433 c        15197 g        24422 t
ORIGIN      
        1 ttatctaacc taactaacac aacaaatttc gggtcataag caggagcaaa accaacaaag
       61 ctttgtcaag tttcattaga ataactggcc ttatcaatac caaaacttgt atacggaatt
      121 tgcgaagtgc cggtttttcc agcaatatga taaccaccta ctttagcaag ttttccataa
      181 ccattttcaa caacgctgac aagcattttc gttaaagtcg aagcagtttc ttcgggtggg
      241 ccgggtagga ttcgaaccta cgaagacata cgccagcaga tttacagtct gccccagttg
      301 accgctttgg taccgaccca aaaatcttgg agccgtcgtc gggacttgaa cccgagaccc
      361 cttccttacc atggaagtgc tctaccagct gagctacaac ggctaaatta taactgtctt
      421 atctgttcta aataatctgg caaatgacca ttaccaggat caacggcgga taaagtatcg
      481 aaaacagtaa ccgccgtaac ccgatccttt ctcattatac taactgccag catcgtgtca
      541 agatagcgtg gatttttagg ggctaatttc aaagcttgtc ttaaataatg gtcagcccgt
      601 tctaactcat cactacggta gtatgactgc cccagaagat aatgatactc agctaatttc
      661 tgtttagtat ttttagcctc cgggtggcgc cgtaattctt ctgcccgata tttttgagtt
      721 aaacgtttca caatataatg ccaaacttct tgggcttcct tatacttttt ttcttcattg
      781 tagacattag ctaaaaaagc aaaactatct atctgacttt ggtctaactc taatgcctcc
      841 agggccaaat gttcagcctc actgaaacgt ccttgagata aagcttcctc agcatcaact
      901 agcagttctt tggctcgttc tctatttaga ccccgtgact gcaacttagt tttaacttgt
      961 tgccgatgta atcggtttag cttagcatat ccccgccaaa aaaattcctt tattttagta
     1021 taaagagatg ccacaatatt acgccaccac caactgtgtt tagctaactg ccgacgaata
     1081 cggtcattaa taatttcctg ctttttagcc gccgcctgtt ccgctggtaa attatctaca
     1141 tcaatattag ccaatagcgg cattttatga aaaataataa ataatacaat cgccaaggca
     1201 ataattaata aagcgataca cagccacatt aaaaacataa accttaagca aagactatgg
     1261 accaaaaact atcagccgac aaactagctc caccaactaa taggccatca atattgggtt
     1321 gagataataa ttgaccagca ttttctggtt taacgctacc accatataat atccggttac
     1381 cctctatccc taattctttt aagaattgtc taataaaatg atgcatagcc tcagcctgcg
     1441 taggagtact ggtttttcct gtgccaatag cccaaatagg ttcataggct agtactacct
     1501 cccgtcccgc cggcactact acggaattaa aaattgccct aagctgtccc cctaccttct
     1561 cttcagcctg accagcttcc cgctcagcta aagtctcacc gacacagact atgggaatca
     1621 aattagttcg ctctaagaca gcttgcagtt taagtctaac taactcatca gtttccccaa
     1681 ataattgacg tcgctcacta tgacctaaaa taacataacg gcaatcaagc tcaaccaaac
     1741 tttctaaact agtgccacca gtaactggtc ccggtacaaa ttgggttgca tcttgtgatc
     1801 cccaaaaaat agctgtacct tctaaaatat tggctaccga agacagggcg acaaaactag
     1861 gacacactcc cacttccgcc agaagtggtc gatatggttg gcttaagact aaattttcag
     1921 ctaaagttac actttcttta tagcctagag ccattttcca attgccaata attaacttgt
     1981 gcatataaaa atatttatgc cattattcta gcatactaat aaaaattaac caattaataa
     2041 tatttttcag tgatataatt taacaaatcc ttagtaatag taaaccgccc ctctttagtc
     2101 ggagccccca ggacaacagt aattaactct tccccttggt tattacgcca tagggcggca
     2161 aaacaataac cggcttcatc taaatagcca gtcttaccgc cgagaatctg ccagctatct
     2221 ggtgccgcta ctgaaccccg aataaagcca tcagtattag tgactagctg ccgccggcct
     2281 tgtttagtaa ctatctcata ttgctctaat aataatgtct ctctaatttc ggactgcgac
     2341 aaagcggttt taactatagt ggcaactgct cgcgcactag cttggttgtc aggactcaag
     2401 cccgtaggct caaccaaatg aagatcagag gctaaattaa acttacgagc taattcttct
     2461 actttttgag aataagaagc atcatcaagg cccacggccc gaattaacgc ttcagtagca
     2521 gtattatcgg aggccagtaa actagccatc cataaatctt taatagtgac cacctcacca
     2581 ggataaatat agatgatatt gccataacgc tggtcttcat ttttcaaggt atactcagct
     2641 gaccaattag ggtgagtatc taagacaatc agggccgtaa ctaacttact taaactagca
     2701 ataggacgca actgatagct accttggcta agaagcacac cttctttatt agaaataacc
     2761 actgtgctac tagccggcca agccttaact tccggccagt tattgggcca gacaaggggc
     2821 acttccccag ctaaaacaag gggctgacta gaccaaataa cggccatgag ccaccaggct
     2881 agattaacta ataaaataat aatggtgcgc tgtaactcca ttgtttatta cttaagaggc
     2941 tgttctactg ggccaccact atcctcccaa ataacttgtt taatgcattg ataaccggct
     3001 tcatggggcc atttttctac cccgacagtt ctaacacctt tatctgggtt aatattatta
     3061 gtatgccata aggcgcaaag ttcatactga ttattcccta gcccccgata gcgtatagcc
     3121 tctccctcac tcgtttttaa ggctgccgga tctaagtaat aaggacttac tgttaattct
     3181 tgtaaagtcg ctggtaaatg tttattttct tggtaataaa cattaagagc actatctatt
     3241 tggtaaaaat tgccaattaa tttttcatca tagcgacgat tccgagcctc cgttggactc
     3301 tcggcaatcg caaagccgcc aataataaga ccaataatca ctatagcgac tggccaggca
     3361 aaagagtgaa ggtggtattt atcactaggc gattgaggat tacggtgcag gtcataatga
     3421 taaaagccaa aaatagtagc ggcaatacca atggctacta tagctttagc taaaaactgc
     3481 ccagtcaact caccatctaa ataatgataa actaaagcaa caagccagcc ggacatcaca
     3541 atagctgtaa ctaataaaat aaaataaata agccaacgcc gtaaatcaga ctgaggactt
     3601 atttctcttt ttactaatcc gcgattaatt tgactcacag cccaaaaata tagtggggta
     3661 gagattaata aagcggatag gccaaacttt aaactagacg ccgtatagct tatggtatac
     3721 cccgtaagcg gatcaggtag ccatttatta attaactgaa aagctactac tcctgctgag
     3781 attgctaaaa aaattaacga aataagggat aataaataaa gaaaagtaaa tttgggtaaa
     3841 gaattagctt ttttggttgg catataatta ttttttagta tcttgacgcc aggataaata
     3901 aaggtcatca agctgatttt ggctaacttc acttggactc cccatcagta aatcccggcc
     3961 ctgagtatct ttagggaaag catagacatc acggatagaa tcataatctt ttaaggccat
     4021 cagcaagcgg tcaatccccg gagcattacc gccatgaggc ggagcaccat acttaaaggc
     4081 cttaagcaaa gctccaaaac gacggtcaac ctcagccggc tcataaccta ctaaccgaaa
     4141 agcttcatac attacttctg gttgatgatt acgaatagcc ccactggaaa cctccactcc
     4201 atttaatatt aaatcaaact gataagctac tatttctaac ggtggctgat tctttaaggc
     4261 ttctaatcca cccttaggta gagaaaaagg attatgggaa aaattaagcc cggtcggagt
     4321 actgcctact tcataaaaag gaaaatcata aatccagcac caagctgcta accctggatc
     4381 ttttaaatta aaccacccgg ctatctcttc ccggatagct cccaaggcct cacaggcctg
     4441 aagtcctggg ccagctgtaa ttaatattgt ctcgcctccc tttaaggata aatgcttaac
     4501 gagggatagc aaaatattgt cttctaattt agtggctaaa ctgccctgcc aattttcccc
     4561 tagataaatt aaggaactta aaccactcac ccctttagtt tttgctaatg ccgttagctc
     4621 atccaactgc cgtcggctta atttttcagt ctcaggcaaa actaaaccat aaatatggcc
     4681 gccactggct aaaacatctt gaaagatact taaatggctg tgagctaata cttgactcaa
     4741 tggtttaata gttagatcat aacgtaaatc tggtttatct gtgccataag tttccatagc
     4801 ctcacgccat gttaattggg ggaaacgtcc atccttgagg ccaataactt ttttcgagct
     4861 aaattcagat gtcagctcta gcattaatgg ctccataatc tgccaaatat cttcttcact
     4921 atctacaaaa ctcatctcca tatctaactg gtaaaattcg ccataatggc ggtcctgccg
     4981 gccatcttca tcacgaaaac atggggcaat ttggaaatat ttatctactc cgccaatcat
     5041 taaaagttgc ttaaactgct gaggcgcttg cggcaaagca taacaatgat acggaaataa
     5101 ccgagaaggt ataagaaaat cacgcgcccc ttccggagaa gaattagcta aaatcggcgt
     5161 ttgtacttcc acaaagccta aatcttgaaa atattgacgc attcgccgaa taaataaatc
     5221 acgggtgata agcatctcct gcacactggg acgacgtaaa tctaaaaaac gatattctaa
     5281 gcgtaaattt tcattgctaa gccccacttt atcctcggct aaactaaagg gcaagggtaa
     5341 agaacggctt aaaatctcta gatcatcaac taccacctct atttccccag tcggcatcgc
     5401 cgggttcacc atttccactg gccgtaaacg tacacgacca tgagcccgca caacaaactc
     5461 atcatgtaac tcctcagcaa taacggccac tttcggctct tgagctaaat ccaccaccag
     5521 ctgaatcaaa ccgctatggt ccctcaagac aataaacatt aggccgccta aattacggct
     5581 tgattgtacc cagccggcta cataaactgt ctgatcaact tctgtggccc gtaactggcc
     5641 acaagtatga gtccttaaca taaagctaaa aaattaagcc aactattaaa aaaagcagtt
     5701 aactcttcta agattgtagg cgatgatggt aatttcgtca attgtaaatg ttcatcctga
     5761 actaaatctt cccaaattac taaagtactg ggagatagct tatacgttgg acctttgggc
     5821 tgacagacct cacaaaaaaa tccgccctgg gtctcactcc agaaggcggt actaagtggc
     5881 cggccgcact ggatacaagt atgtcccggc gcataaccta attcccgcgc taaccgccac
     5941 gacataattt ggctacctac cgttagttga gacaaagaat aatgatgact attccactga
     6001 gttaagatgg taattaaatc ctgccaggaa cgtggtaggg gctgagctaa tggccaccac
     6061 tctataaccc gccttactaa ttcagttgat acaacaatta aggctaaatc acttttaagc
     6121 cccagaaatg actgccggct caccgctgcg cttaaaagcg gctctttgct ccaaacaata
     6181 aggccgtcaa ccaaagataa gggctctaaa tgcccactta gtttagattg aggcttaaat
     6241 aaacccttgg cggtaaatac taaacggccc cgctccggac ttaaagccac aattttcccc
     6301 cggtaatccc tatcctcagt ccgtttaata attaataaac gttcagtggt cgtacgccgc
     6361 ataattatct aaaaaagttt gtaaaataag catagcagca atagcgtcct caggagcagc
     6421 tgccctagac tgatgattaa ttaaccgatt agccccctga ctgcttaaac gttcatctac
     6481 taaatgtaga gggtaaccac tatgtttaat aagttccacc aattcctgcc agccctttaa
     6541 atacttaccc ctctctccac ctaaatgata aggctcaccc ataactatct ggtcaacatt
     6601 ttctcgctta atcaccgctt ctaccgccgg ccaatccttc actaccccat aaggaaaagc
     6661 taacttagtt tcgctatccc ctagggctaa gccaatcttc ttttggcccc agtcaagacc
     6721 taaaaaaata ataggttcgt tcatgtctag attgttaata tttgatggcc tgtggtggtt
     6781 acggcaatag tatgctcaaa atgagcactt aaacttccat cagccgtctc aaaagaaaga
     6841 ccatcacggc ctactttaat ttccgggctg ccagcactga ccataggctc aatagcaata
     6901 accataccag gctttaacac aatatcctgt tcaccatatt cattgcagac ataatgagga
     6961 atagagggat cttcatggac tgcgagacct actccatgac ccaccaaatt atggaccaca
     7021 gcaaaaccat gttgctctac caaaccttca ataacttggg caatctttag taagctacaa
     7081 cccggcgcta cttcgttaat agctaaagct aaagattctc tagtaaccac aattaaatga
     7141 ttagctaaac ggctaacctt tcctaccggt acggttaccg ccatatcggt aaaatagccg
     7201 ccatgtggag cactagaatt aaccggccaa ctaggtaagt agcctggttt ttttaaggga
     7261 tattccatcc ccacgtctag gccaataata tcgcctgagt gtaaaactcg agccggaata
     7321 gcaggaccat ggacaatttg ctcattaata gaagcgcata gaccagtagg aaaaggcctc
     7381 tcccgtcggc tggagcgaaa acccttaaac gatggagctc cgccagccgc tgctactaat
     7441 tctaaaaaca aagcttctaa atcggcggta gttacccctg gtttaacggt tgctgctaat
     7501 ttttgagtaa tcccggctaa aatatgcccc ccttctttga ttaacgcaat ctgctccggc
     7561 gttttatacc taatcataga ttatcttact taatggggca agacatagcc ccgcctagtt
     7621 aaaacagcaa taatatcctg atgaactgcc ggaatatcct gctccccgtt aataacttcc
     7681 agttcacctc gcttctgcca taaagcaaga agcggctcca tggtgtcatg ataaattttt
     7741 aaccgttcag caataacttc tggcttatca tccggccgtc ttactaattt ggaagaacaa
     7801 acatcacaaa aaccttcacg cttactaggt ttatatatta gatgataacc ggctccgcag
     7861 tgcggacaaa accgccgccc ctccagcctc cgataaacct cctcatcact taccctaatc
     7921 tctaaagcac aaaccctgtc ttggggagta ataatataat tataggcata agcaaactgg
     7981 ctgctattgc gtggataacc atctaaaata aaacctagct tagtatctgc ctgagtcaag
     8041 cgacgctgaa gtaaatcatt aatcacccca tcatctacta aatgaccggc ttgaatagct
     8101 tcttgatatt tttgcggtaa atctgcggag cgacgcaaca aatcaccact ggaaatagcc
     8161 ggccaaccat aatatttaga gactaactca gcttgcgtcc ccttacctga agctgggggg
     8221 ccaataaaaa acaaaatatg ccttaccata aaattgtatt aacccctaca tcataccaca
     8281 actgagatgc caaaactaga gtaataacaa agataaaaat aacaattcta gaaagaactg
     8341 ctagctaacg gatgcaatga atctcattaa caatggatat taatatccat catattgacg
     8401 catagtcatc tgcgcctcaa tctgtttaac tgtctcaata actactgaga ccacaattaa
     8461 gagagaagtg ccaccaatag ctagggcttg taccccagta atcgagcgca ttattaaagg
     8521 caaaatagca atcaccccca aaaagagggc accagctaaa gtaattttat gcattatatt
     8581 ggctaaatag cgggccgtag gctggccggg acgaatgcca ggaataaaac caccttgttt
     8641 ttgtaaattc tcggctaccc ggtcgggatg aaaaaccacc tcggtataaa aataagtaaa
     8701 aactaccact aagataaaat aggcaatacc gtaaacccat tgatcctgaa tagctttaat
     8761 tacccaagct gaagctgtgg ccagccaagc caccttactt gtgacaaaaa attgagcaat
     8821 catggaggga aacatcaaaa tagaaactgc aaaaattatg ggaataacac cagccatatt
     8881 aacccgcaaa ggcagatggg tagtacttcc cccataacta tgaccgccat gcatctgccg
     8941 ggcatactgg actgggatat tacgctgacc ctcggtaaca taaaccaccc ccaaaatagt
     9001 gagaatggcc acaataataa agcccaaaat cataaaaaac tgggagctat tataggtcgc
     9061 aataatctgc tgaaaagatt gaggtacgcc ggcaataata ccagcaaaaa ttaataagga
     9121 aataccattg ccaattttct tttcagtaat aagctctcct agccacatta agaaaattgt
     9181 gccggcagta atggtaataa taatagtaat aaaatttaaa ggatctaaag cacctaaaac
     9241 ctggtagctc gaccggcgca gtaaagtaat catcccataa gcctgcaggg cggcaagcgg
     9301 tactgtggcc caacgagtcc acatattaat cttttgccgg ccagcttcct ctttattcat
     9361 ttcttcaata gagggaacaa tcatccccag taactgaaaa ataatagaag aagtaatata
     9421 aggggcaaca cccatcataa caatagaaaa attctgtaag ccaccaccag acaataagtt
     9481 catcataccc agcaactggt tggaagcgaa taactcccgc aaagcttgag cattaacacc
     9541 aggaatagga atatgggcgg ccaaacgaaa aactaccagc atcgctaaaa caaagaggat
     9601 actgttcctt aaatcacgca ttcgccaaat ttttaaaatt ttctccatca tagattattt
     9661 attatctagc tgaccaccta atttagtcaa ctgctctttg gctgccgcgc tcacctttaa
     9721 attacgaact cgcaagtttt ttaacttcag ctcaccgcgt gccaataatt tgattggctg
     9781 accagacttt actaatttag ccttgactaa actttgggga tcaatgacag caccatcttg
     9841 aaaatgatga ttcaaactag agagattcac tacttgagct ttgggcttat ccgaatgaaa
     9901 gccgcgtaat ttaggaatct ggagaatatt ttgtcttaag gcccgacgtt ttaaatcact
     9961 tactccagac cgagctttct gacctttaag accacgccca ctataattac cgtggccgga
    10021 agaattaccc cgtcctaccc ggcgtcgaga cttcttagcc ccactatggg gttgtaaagt
    10081 attaagtgtc aacataaaaa tattacatta agctttggcc gctgctggta ctactttctt
    10141 tgaagattta gcactatcag ctgtcgctac atttttatcc tgtttgtcct gacggactgg
    10201 cttttttaaa ctttgtagag ccttcatcgt gcacttagca ttattaactt tattatgaga
    10261 accaatcatc ttgctcgtta cattatgtaa accagccaat tccaaaatga ttcttacaac
    10321 actgccggca ataatacctt taccttgagt agctggcttt aagataatta tggccgcatc
    10381 agatttctgc cgcacttcat gcggaatagt gccgtttatt atggggacag tgatgagact
    10441 tttcttagct tgattaaccg ccttattaac cgctgtcgtt acatctaagc ccttagcaag
    10501 accgatcgcc acccggcctt tgtggtcgcc aatagcaaca caagcccgaa aacgcattct
    10561 tttgccaccg gccattaccc tggtaacccg agcaatgtct ataattttct ggtcaaattc
    10621 atctggttgc tgctctgagc gtccgccctg gcctcgacta gtcgaaaacc gtctctggcg
    10681 attatttctt ggctctgcat tcggagaaat tgttgcttta atatctttca tataaattta
    10741 ttattaaaat tgtaaaccag cctcacgggc tccttcagct accgcttgca cccggccatg
    10801 ataagcatta ccgccccggt caaaaactac ctgcttaatg tccttagcta acaatttttc
    10861 cgccaataac ttacccaaag cccggctaat ctcagtttta gtaccagcta acttaatctc
    10921 ccgactatga gcactaacta aggtttgacc ggtagtatca tcaatcgcct gaagatacat
    10981 accccggttg gaacgaaaaa cactcaagcg aggacaagta cccgtaccct taattttagc
    11041 gcgaacccgt ttatgtcgtc taattttatt aactcttttt tcgctatgca tattcctagt
    11101 ttaatttact tagccgctgt cttacccgct ttacggcgaa taacttcatc agcatattta
    11161 atacctttac ctttatacgg ctcgggtgga cgcagacggc gaatctgcgc cgccacttcc
    11221 ccaactaatt gtttatcttg gccagcaata gttattacat ttccatctac agttgctgta
    11281 ataccggaag gcaaattaaa ttcgactgga tgagaaaaac ccaaactaaa agtcaatttc
    11341 tgaccagcga cattaacccg gtagcctaca ccattaattt ctagtttctt ttcataaccc
    11401 ttagttactc caatgaccat attaccaatc aaccggtgaa ataaccccca taaagcccgc
    11461 tctttgcggt cctcccgatt agccacatta accaatattt tatcctggtc aatggtaact
    11521 tgaacttcag gcaaaatagc ctgttgcaac tgacccttgg ggccagtgac cgttaaaatt
    11581 ccagcctgca tcatagctgt tactcccgat ggtaagataa ttggcaattt tcctagtctt
    11641 gacatatagt ttttctaact cacattgaaa ttaataaacg ctacaaagca cctcaccgcc
    11701 taaacctttt tcccgagctt ctttattggt taacaatcct gccgaagtag aaattatagc
    11761 aatcccgaaa ttattaacga ctaccggcaa tttatcttta cttgcataaa cacgacgacc
    11821 aggccgacta atccgcttaa ttgaagtaat ggctggccgg ccattggctt gatactttaa
    11881 aactaacttt agactagcaa aattagcggt cttattttta gttagtggcg gattaataat
    11941 ctctacggcc gctaaatagc cctcacgctc taatatttta gcgatattat actttaattt
    12001 actcatcggc aaaatcacct cggttttatg tactgccgcg gcattccgaa ttcgagtcag
    12061 catgtcggaa attggatcaa tcataaaaag ataaatttag taatttgtaa atattaatta
    12121 ccagctagat ttagttatcc ctggtagatc accactactc gctaactctc gaaaacaaat
    12181 ccggcaaaga ccgaagtccc gcatataacc tcgattgcga ccgcaacgcc aacaacggcg
    12241 gacgacacga gtagaaaatt taggagtctt tttagctttg gcaattaatg cttgtctggc
    12301 cataatttaa aaattaagtt atagggcttt aaaaggaaag cctagtaatt taaatagagc
    12361 taaaccctct tcatgggttt tagccgtagt tgctatggaa acctgtaatc cgtgctcctt
    12421 ttccagctca tctaggcgaa cttctggaaa tgaagaacat tccttaaatc ctagattcag
    12481 attaccttgc tggtcaacta ccccactgtc taaaccacga aaatcgcgga ttctgggtaa
    12541 agaaatagca attaattttt ctacaaaatc atacattcgc tgaccccgta aagtaacaga
    12601 aacaccaaca attgcccctt ctctaatttt aaaggcggaa atagatttac gagctggatt
    12661 caaaataggt ttctgaccag taatcttcgt taaagtatct tggacaccat caataaaggc
    12721 cttatcttta gccattcgcc ccacccccac attaaccact accttagtaa gccgaggcac
    12781 gctcaactta ttagaatagg aaaattgctc catcaaggct ggaataactt cctgtttgta
    12841 tttttcagat aaacgcatag acataaatta actaatcaat aaattcttta caccgccgac
    12901 agattcttac cttacgattt tgcgccttct gtccttcagt taccttaata tattggtaac
    12961 ctacacgggt cggcttgcca caatgaggac aaattaactc taaattagaa atatgtaacg
    13021 gcgctggaaa ttcaattcgt tgccctacct ctcctcgatg ttgcggccga atatgtttaa
    13081 ttaataaatt aataccttcc acactagccc gctgctcctt aggaaaaacc tgaagcacct
    13141 tgcctctttt gccacaatcc ttaccgctaa ggactaagac ttggtcattc ttttttattt
    13201 tcatatacta ttaattataa tacctcaggg gccagtgaag cgatttttac aaagccagcc
    13261 cgccgaattt cacgaggcac tggtccaaaa atacgagtac cacgtggctc atgagttttt
    13321 ttatctacaa taacacaagc attatcatca aaacggatat aagtaccatc agagcgacga
    13381 atttctttcg tagtcctaac aataacggcc ggcacaatat cactcttttt aatactagta
    13441 tggggcatag cctcttttac ggtcacaaca atcatatcac ctacagaagc atagtgacgc
    13501 cggtagccct tcattaagcg aatacactgg acttttttag cgccggaatt atcggcaaca
    13561 tttaacatgg tctgaacttg aatcatataa ttaacaaatt aacggccatt cttgatcacc
    13621 cgccagcgct tttctttgga aagtggccgg cactctacaa aactaactcg gtcaccctta
    13681 tgataaaggc ctgagtcatc atggacttta taattccgac taaccacata acgtttatgg
    13741 tacttagggt gaagcttaac tcgttcaacc ctaactacta gagtcttttc cattttatcg
    13801 ctcaccacta ttccttgaaa tttcttgtga atagcctgac gccgcggagt gaccactggt
    13861 tgtggcttct tagctttact ggccgtttta cttggtttat tagtcatatt atttcttctg
    13921 gcgattatta agtaaagtca aaagacgagc aattagctcc ctcgttgctc ttatttcccg
    13981 cactgattta agttgcccgc tagctgcctt aaaccgtaaa tccttcaagg caactcgctt
    14041 ttcggctaat aatagtcgca attcagtgtc agttttttct tgtaactcgt ggtaattcat
    14101 aagatcagtt aattaacttg gtcccggcta acaaattgac atttcaccgg caatttatga
    14161 gcggctaatc tcattgcttc ccgcgctttt gactcatcta tcccctcaat ctcaaacatt
    14221 atggtccccg gcttcactgg tacaatataa tgatcgaccg aacctttacc cttgcccata
    14281 ggaatttctc cgcctttagc agtgactgga tggtcaggaa aaaccctaat ccacaacttg
    14341 cctgttctct gaacataacg agtaataacc cgacgagcgg cttcaatctg ccgtgaagtg
    14401 acccaacaag cagtagtggc tttgagtcca taactcccaa aactaacagt agtaccgcga
    14461 gtggcaacac ctcctctctt ggctttatga gacttccgat atttagtctt ttttggcatt
    14521 aacataaaaa ctccttaaaa ttttaacgta aacgttgacc cactacctca cccttaactt
    14581 cttcagtgcg gccaaacact tccccacgat acacccacac tttaacccca atagtgccgt
    14641 aagtagtatg agctgtacct ttggcgtaat caatatcggc gcgcaaggta tggagtggca
    14701 ctttgccgga agttaaagac tcggtacgcg caatctctgc tccattaaga cgaccaccga
    14761 ccatgacttt aatccctaaa gcgcctgacc gttcagctcg attaatagtc tgtttcatca
    14821 cccgacgaaa tggcattcgt ttttctaagt ccataaccat actttgcatt aagacctgag
    14881 cacttaaatt gggacgattg acttcactaa tatttaaagt aatatcatga agacgaaaat
    14941 ttttcaaaaa acgattatgt agagtcttct ttaattcttc agccccagct ccaccccgac
    15001 caataactaa accaggttta gccacaaata aattaatagc taccttatta gcgctccgtt
    15061 caatttctac tcgatccact cctgcctctc ttagttgccg aaagagataa tcacgtattt
    15121 taatatcctg ccgagcatta ttaatataag acttaccacc tttaaaccat ttagaaggcc
    15181 aagtcttgat aattcccatg cggaaaatag ttggattaac tttttgtccc ataaaaatat
    15241 attaattaac cggatttacg attaaaaacc tttttaacaa atcctgattt tgagctacct
    15301 tcctgcttag cagctttctc taaattctca cccatagttt ccaccgccgc tacctcttta
    15361 gtggtgtctt tactagtctt agctccaccc ttaattaaat cacttaatct caatggagcc
    15421 gccacctctg cccgccgagc ctttcgcgga ccactatcat gaatttctcg taatactact
    15481 gtaatatgac tagtttcttt acgaatagga gtagcccgac catgagcctt gggcgcccag
    15541 cggtgaaaag tcgccccctt gccgacccaa atctctttaa tcaataaatt gtcggcggcg
    15601 atatcataat tgtgtttcgc gtcagctaag gctgatttta gtaatttact tattaatcta
    15661 gccgcttttt tattagctac cttaagctga gccaaagcct cctgggcttg acgaccacgc
    15721 actaaattag ctactaaatt agctttacgg ggcgaaactc gaacatattt tgctttagca
    15781 gtaatctcca taaaatatta tttagttgct cccttagttt ggcttctggc cattttacca
    15841 ccatgaccaa gaaacttacg agtatgagca aattcaccta atttgtgacc aaccatattt
    15901 tcagtaataa atatagggat atgttgtcgt ccattatgta ctccaatagt aaaaccaacc
    15961 atctcgggac taatagtgca agctcgatcc caagttttaa taatagtttt atcgcctaag
    16021 ggcaacttct taattttggc taatagccgt tcattaacat aagggccctt tttcaaacta
    16081 cgtgtcataa ataacaataa aataataatt aatgtttttt ctgccgacgc cggataatta
    16141 aggcattgtt tgccgcctta ggattacgag tacggactcc caaagcatgc ttaccccatg
    16201 gagttttagg atacttcaaa ccgataggct gattaccttc accgccacca tgcggatggt
    16261 cattcggatt cattgctgtc ccacgaactg ttggccgaat ccctaaatga cgagttcgtc
    16321 cagctttacc taaattaatt ctcgctaatt caggattact cacttgacca atagtacaaa
    16381 gacaattttt atctactagg cgaatttctc ctgagggcat cttaagctga gcataacgtt
    16441 tatctacacc catgacataa agaacattgc cagcggctcg ggctaacttg cctccaaaac
    16501 caggccgtaa ctctacatta tgaaccggca cacctgccgg aatatattct aagggcaaag
    16561 cattaccagt tttaatatcg ataagcttct tagaacttaa aacttcatcg cctactttaa
    16621 gacctagggg cgcaataata tagcgctttt ctccatctag ataatacact aatgctaacc
    16681 tagctccgcg gttaggatca tattcaatgg ccttaacgcg accagggata tcaaacttat
    16741 ctcgcttaaa atcaactaat ctaatataac gtttagaccc gccaccgcga tgtcgtacgg
    16801 taatcttgcc ttgattattt cggccactat gttttttacg aataactaat aactggcgct
    16861 ctggggtagt gctggtaata tcgctaaaac tatcgtagct agcgccacgc agtccaggag
    16921 ttattggttt agcttgtcta atcgccataa atcttaatta aataaattac accccctcat
    16981 aaatcgcaat acttttacca gctggcaaag taacaatagc tttcttccag gccggacgtt
    17041 gtcccttaat tcgcccccgt gtcactttct tacctggcat attaataata ttaacagcta
    17101 ttggtttaac cccataaaaa gcctcaatgg ctttagcaat agtaatttta ttagcccccc
    17161 gctctactac aaaaacatat tggttaagct cattaagcgt accagccttt tcggttactg
    17221 ctggtttaat taaaatactg gctagttgtc ctcttttcag ggtctgacga tcagtcttaa
    17281 taatttgagc ctctccctgg gtttcattac tttcagttgt tttattaatt ttatttctct
    17341 tgtccataat attttaatta acgttctgac cataacgcgc ctgtaaaata tcaataacag
    17401 gctgagtaat aataagttta ggcgctcgca acagactaac taaattaata ttttgatcat
    17461 ttaatactct aaggtgcggt aaattatggc tagctaactt tattgactca ttatctacag
    17521 tagttattag taaaacttgg gaatgttttt taacctcatc tgattcttga gccgctaata
    17581 atttatctat tatctctttt aaagctttag ttttaccctc cggaacggtg aggctatcca
    17641 gaataattaa attattagtt gctactcgat cgctcaaaac catacagata gctcgagcag
    17701 ccatcttttt attaattttt tgcgagaaat tacgatcttt agtaggaccg aaagtaactc
    17761 caccgccaat ccacaatgga ctacgattag acccagcccg agctcggcca gtaccttttt
    17821 ggcgccatgg ttttttacca ccgccactca cttctccccg atctttagta tcggcaataa
    17881 cccgacgggc attagctagc tgagccacca tcgcctgatg cactaatcct ttattaagag
    17941 ctacgccaaa tactgcctca gataattctt gttctccaat aacctcagct ccttgattgt
    18001 atactttata tttaatcata ttattaacta attaagccac ctctaccttt gacttttctt
    18061 gattatctac tactagttcc tcgttgactt ctggctcggt tttagtctct tctaactcaa
    18121 ctggtgcttg gtcagtctta gttataagct cgccactgcc agaaatagct actaaaccat
    18181 tacgccatcc tgggactgct ccccgcaaat aaagataacc attagctaca tcaacctcca
    18241 ctacttctaa atttttcact gtcacttgct gactaccata atggccaggc attctagtcc
    18301 ccttaaaaat atgagctggc ccagtggctc ctactgaacc aggcattcgc tgctggtctt
    18361 tatcaccatg actgcgcccc tgtccttgaa aaccatgacg cttaacaccc ccctgaaaac
    18421 cacggccctt actaacagaa gttacctgta ctaaatcacc tggtacaaaa gtttctatag
    18481 caatatgctg accaacctgt aattgtgctt ccaccccggc cggttcccta tctaaattaa
    18541 attctcgctg gtattgaaaa tttccccaag caccaaaatg gccacgttgc ggtttagcaa
    18601 tattttttac cgcccgtctg cctgtcccca cttgtactcc ccgatagcca tcacgttccc
    18661 tagtcttaat ctgggtaatg acacatggct caaccttaat acaggttact ggaactaccc
    18721 gatcatcgcg ccataattgg gtcatgaaca tttttttgcc taaaataaac ttcatacttc
    18781 cttaaaaact taaataaaaa actggttatc tcaaaaccag tgtgcaaaac caagccattg
    18841 ttttattgtt tagctcttag attttggtaa ctgttttgat aatttttcct tccatttaat
    18901 ctagctatct ccttagaggc ttctctcatt ttaatcaaat gtgaggtttc ctaaggccta
    18961 actaaattaa tactatttaa aataacaatt tacattttaa tttccaaatc caccccggcc
    19021 ggcaaactta aactcattaa ggcctctact gtcttgctag ctgggtcaat aatgtctaat
    19081 aagcgcttat gaatacgcat ttcatactga tcacgagaat ttttatgaac aaaggttgaa
    19141 ctattaaccg tatatttctt cttctcaatc ggtaagggaa ttggtccata aattttagca
    19201 ccagtacgtt ccactgtttc aataattgtt ttagtggatt gatcaataat cttgtgatca
    19261 aaagcgcgaa tcttgattcg aattctctgt ttatactcag aagaagcaac ttttttgtca
    19321 gacatggttt tggatattta ttttataaac aacagaatag tcctgctacc gctaaggcag
    19381 caggactaac tatctactta ataatcttag taactacccc ggcaccgacc gtccggccac
    19441 cttcacgaat agcaaatcgc atcttttcat ccatagcaat tggggcaatt aacttaactt
    19501 ttaaggttac agtatctccc ggcataacca tctcagtccc ctcgggtagt tgaatctcgc
    19561 cagtaacatc ggtcgtgcga atataaaact gcggtttata tccattaaaa aatggcttgt
    19621 gacgtccgcc ctcctctttg gtcaagacat aaacttctcc ttcaaactca gtatgggggg
    19681 taatagtgcc tggtttagct aaaacttgac cacgctgaac ttcatctttt ttaatacctc
    19741 gcagtaaaat accagcatta tctccagcca tgccctgatc caaagttttc ttaaacatct
    19801 ctactccagt cacaaccacc tttcttgtct ctcccaaacc aactagttct acttcatcac
    19861 caactttaac agtgccccgt tcgattctac cagtaactac tgtacctcga ccttcaatag
    19921 aaaagatgtc ctcaattggc attaaaaagt ctttatctac ttctcgttgt gattcaggaa
    19981 tatagttatc taaagtatcc actaactcta aaataggctt agcatattca ccggttggat
    20041 tctgtaaggc ctgcaaagct gacccgcgaa taataggggt ggtatctccc ggaaaaccgt
    20101 atttctttaa taaatcacga atttcttctt ctaccaggtc aatcagctct ttgtcctcca
    20161 ccatatcaca cttattcaaa aagactacaa tatagggaac accaacctgt ttagctaata
    20221 aaatatgttc ccgagtttgc ggcataggac catcagtggc tgctactacc aaaattgcgc
    20281 catccatctg cgcggcccca gtaatcatat tcttaatata atcggcgtga cctggacaat
    20341 ccacatgggc atagtgacgt ttagctgtct catactcaac gtgggtggtg gcaatagtaa
    20401 taccacgcgt cttttcttct ggtgctgaat caatctgatc aacgctcttc tgggttgctt
    20461 tatagccagc agcagttaaa acagataaaa tagccgccgt taaagtcgtc ttgccatgat
    20521 caatgtggcc aatggtacca acattgatat gtggcttagt gcgttcatac ttctcagtca
    20581 taatttcttg gttaaagcca actagccctt tttagactag tggtcataat tgtttaaata
    20641 aattattatt tatcctctcc tgccaaatct ttagccgctg acttacggct ggccggaata
    20701 gaccaatggg atttttgacg gtcatcaacc tgctccacaa aacttgattt gacagaaata
    20761 ttaccagtat cagaactggc aacattggta cttactttag caaaaccctg accatttgtc
    20821 aagccagcta aatctaaaag tttttcatag tcgcttagcg acaaaatcac taggctactt
    20881 actggatttt cttggtcagc cacaattagc cgatcaccgg ttaattgagc cagccgcaga
    20941 atttgttcca aattattaat cataggttta aattaccagt taatattatt tactagtttt
    21001 accgatgatt acttccgcca cattgcgcgg tacttcccga taatgtaaaa actccatatt
    21061 ataactagct cgtccctggc taatggacct taactgagta gcataaccga acatttctgc
    21121 tagcggcacc cgagcatcaa taactttaag ctgaccacga tcgaacatcc gatcaatttg
    21181 acctcgctta gcattaaggt caccaataac attacccata tattcttccg gcgttataac
    21241 ttcaaccttc ataatcggtt ctaaaagtac gggttgagct ttcttgcaag cttctcgaaa
    21301 ggctaaaata gcggccatct taaaagcaat ttcactagaa tctacttcat ggtaagaacc
    21361 atcataaact gtacatttaa tatctaccat aggatacccc gctagtactc ctttgtccag
    21421 agcctcgcgg aacccctttt caatggccgg aataaactcc ttgggaataa caccaccttt
    21481 aacctcgtca acaaattctg aaccctgtcc ttccggctgg ggtgataatc gtaaccagca
    21541 atgaccatat tgaccacgac ctcctgattg cttgatatat ttaccttcag cctcagctgt
    21601 ttgacgaatt gtttcacgat aagatacttg cggttgcccc acattagctt ctaccccaaa
    21661 ttcacgtttc atccggtcca caataatatc taaatgaagt tcacccatac cagaaattaa
    21721 agtttggcca gtctcttcgt cagtttctac tctaaaagtt ggatcttctt cggccagacg
    21781 cactaatgct acactcatct tttcttgatc agctttagtt ttcggctcaa ccgcaatttg
    21841 aataaccggt tcgggaaaga caataggttc cagacagatc cgctggcctt catcacataa
    21901 agtattgcca gtcaccgttt cttttaggcc aataactgcc gcaatatctc cggcgcagat
    21961 ttcagtaatt tcttcccgat gattagcatg catccgaacc aaacggccaa tcctttcttt
    22021 agcaccatta gccgcattaa tcacataaga gccagctttt aagataccac tatatactcg
    22081 gacaaaacac agacgaccaa cataggggtc agtggcaatt ttaaaggcta aaccaacaaa
    22141 aggaccattg tcatctggtt tgactataat aactttatta ggatcattag catcatgggc
    22201 ctctacgtct ggcaaatcta aaggtgatgg caaataatct actacggcat ccaatactgg
    22261 ctgaatgcca atatttttta aagccgctcc acaaaaaact ggataaattt cattagcaat
    22321 aacttcttgc cgtagagctc tcttaagctc ttcaatactg atctcttcac catttaaata
    22381 tttatcaatt aaagtttcat ctaattcggc aattttttct atcaattcat gatgatactt
    22441 ggttacctcc tctctcatat tatctggtat ggctacttct acaatttgct caccacactg
    22501 accatgaaat tcaaatgctt tttcggtaat taaatcaata actccccgta tagtttcttc
    22561 agctccaata ggaagctgga gagctactgc ccgcggatta agccgttccc ggatcgaatt
    22621 aagactcatc acaaaatcag cccccatttt atccattttg tttacaaagc ataaacgcgg
    22681 gaccttgtat ttatcggcct ggcgccagac agtctcagac tgcggttcaa ctccagcagc
    22741 cccgtcaaaa acagctatcg ccccgtctaa aaccctaagt gaacgttcta cttctaccgt
    22801 aaagtccaca tgccccgggg tatcaatgat atttacctgc ttacccttcc aaaaacaagt
    22861 agtagctgca gaagtaatgg tgatacctcg ctctttttcc tgagccatcc agtccatttc
    22921 cgcttctcca tcatgagttt cgccaatctt atgcttttta ccagtataaa acaaaactcg
    22981 ctcagtaaaa gtggtcttac cggcgtcaat atgggccatt atcccaatat ttctcacatc
    23041 ttctagttta aattcacgca taaccaataa taaagaaaaa aataataaaa atataccaaa
    23101 aactaaccac taaattaatc attaatggtt agctaaaaat tattatacta atgacgggca
    23161 aaatgagcaa aggccttatt agcctcagcc atccgatgca catctaactt tttcttaaca
    23221 gccgcaccct cccctttgat actatctaaa atttcagctg ctaatttttc cgccatggaa
    23281 tgctccttgc gagctttagc cgcggtaata agccaacggc aacctaacgt aaaccgccga
    23341 tcacctcgta cttgataagg cacttggtaa ttagctccac caatccgtcg tccccgcacc
    23401 tctactaagg ggcaaaccat ttttaaggct ttattaaata catgacgcgg ttcttgttta
    23461 gttttttctt taaggatgtc aaaacaatca taaactattt gttcggcgac tgtttttttg
    23521 ccattcctca taacataatt aataaactta gccacttccg gatcgttata tttagcatca
    23581 ccggctattt tccgtttttg agcctgtttt cctctcatac attaaataaa ttaactaaaa
    23641 ttacttagga gctttagcgc catagcgact acgagcctga cgacgatttt ctactccctg
    23701 agcatcataa accccacgca ctactttata gcgtacgccg ggcaaatcct tagtacgacc
    23761 acccttaatt aaaacaattg agtgctcttg caaattatga ccaataccgg gaatataagc
    23821 agttacctcc ataccattag acagacgaac tcgagcaatt tttcgtaaag ctgaattagg
    23881 tttcttcggg gtggttgtcg ttactttaag gcacactccc cgcttgaatg gtgaaccttt
    23941 tttagcaata tttttccgac ggtgtaaaga atcagttgtc acctgaaggg ctggtgtttt
    24001 acttttgtgg cctacaactt tccgcggttt atggattaac tgattaattg tgggcataaa
    24061 caatatcttt tcttaagaat taaaaataaa tctggcaact ttaagccagg tatagcgctt
    24121 agttgcctat taatgtagca aaatagatag attaagtcaa gaagtcatat caacagattc
    24181 ataagccagg tcacgggata atagattagg tctggaaata ctatgatgac aattaacaaa
    24241 ataagaagac tccaacgttc cgacgctaaa taggaatacc gccagcgttc tggtaaaaca
    24301 agggctaaaa ttttagaacc atcaagggga ggaatgggca ataaattaaa aaccataagc
    24361 accacattaa tcatggccgc cagaaataaa agctgccccc aatccgaatt aggaccacca
    24421 gccagataac gagtagctac agcagcaact atggccaaaa taaaattagc cgctggtcca
    24481 gctagagctg ttttagctac acctttataa ccaccgcgca gattattaaa attaataggc
    24541 actggcttag cataaccaaa aactactgga gaaccaacga atagtaaaag ccccggtaaa
    24601 ataatagagc caaataaatc caaatgaacc aaaggattca acgttaaccg gccagctcga
    24661 gtggctgtat catcacccaa tctagccgct acccaggcat gactatactc atgaactatg
    24721 gtggataata atagaattat aataagaaaa agattcatca taatattaag tgaactatta
    24781 tagattaaca cgccttgatt attttggcaa aacttgttaa gctaaaccca acttataaat
    24841 tactttcatt ttactaataa actaaactat ggctaaaaaa tgtgatatct gccagcgtag
    24901 cgctactaaa ggggctagcc gttctcattc caacatcaaa accctcaaac gacaaggcat
    24961 taatttacaa aattataaaa ttggtgggca aacagtaaaa atttgcacca gctgtctaag
    25021 gacggctaaa aaagtaagcc agaagaaaac tagctaaatt tcaatacttt cttgtcaatg
    25081 tttagataac tccacaacca gtggagttat tttttttgag ccatccgtcg ggcttgaacc
    25141 gacgacctac ggtttacaaa accgttgctc taccaactga gctaggatgg cgaaaaaaca
    25201 atatgaataa ttcccctatt ttttatattg ttaaacaact taactattaa taacaaccaa
    25261 aaattttgcc aactgagata gttaaatatc tttgttgaat aattatctac ccaccaacat
    25321 agtgtcgcca ttccatatct gtaaagtttt ggtacgccgt acaggattcg aacctgtgac
    25381 catctgctta aaaggcagtt gctctaccac ctgagctaac ggcgcaaaaa ggctaaacca
    25441 aatctagcaa aaaagtttaa aaaaaacaag gggcactatt ctcggtaaga cttcagatca
    25501 cggcgcaaat gtttaagact cattaaaaaa ttaacagtat ctttagccag tttaatcttg
    25561 gttggccttt tcccgtaacg gttatccacc cagtctactg gtacttcctt tatccgccag
    25621 cctaaacgtt ctgtccaagc aattaaagct gtatctaaaa aataatccga ttcccgcaga
    25681 aaaggacgaa ctgcccgcca tagttcgtga gacacaaatt tacaaccaca ttgatgatct
    25741 ttaactgtag tacctaaaac atgtcgagct aatttattat ataatgccga gacaataccc
    25801 cgataaaaag cccgcttaca agaagctccg ggtaaaaaac gagaagcaat taccagatca
    25861 gccgatttat ttactataac cgcaaagagt ttggctaaac tttcaaaaga aatagataaa
    25921 tctacatcaa taaaacccca ccaatcagtt agactagtgt cggcataatt taacaaagca
    25981 cgacttttac cggccaaagg ataaactcga gattccacca gaccaggata ttctttagaa
    26041 agttgctgag caatttctgc tgatttatct gaggaaccat taaccagaat aacaacatga
    26101 caatcaagac cataatcttt agccgtaata aaatctataa ggcgtctgac attatctctc
    26161 aaaatagcct cttcattata cacggggagg cataaatcta aactaggcct gctattgccg
    26221 gacataaatc atggtggtag tatcggaatg aaccaagcgc caggccggcg acttcagcat
    26281 ggcccgccgc actatatcag ataaagtata ataatcttcc tctttaagcc cccgccccaa
    26341 taaacaagtc tcaacccaat tataatgaac cggccgaggc tgaggaataa ccattacatc
    26401 taagccataa tcatctagtg ctttcttaac ttctttttct ttaaaaaatc gcccataatg
    26461 ctcaattaaa ctctgaccgc gccacatata ttgaggcagg cgaccatcaa taaaaatagg
    26521 cttctcaggc caacgccaat aaaaatatcc accccaacca tattcaacaa tcgtaggcca
    26581 attagcatat ggcgactgtt gtaaataact aatagctaaa caaggataaa cttcacaata
    26641 atctttttct ggatgagttg accaatgagc atctaccaca agcagaaccg tagtcagaac
    26701 aagaattatg gcgacaaacc attttaaata agccggccag tcatccggcc ggaatgcatt
    26761 atcatgccag gcatgataga aaaatcccgc catccagggt aaagaaacga caacaaaaag
    26821 cgggaaatga cgtactgagc accaagctgc tatacataaa ataagagata aaataagagt
    26881 ccaccaatct tgttgcttga gccaaagagc cacttcacga tcatgctggt catgaccaca
    26941 acaataaaaa attaacaaag ccaaagcaag accaaaaata atgaagtaaa ccatttgcca
    27001 gctatgtata gcttcactta ttggtaacca ctcattaatc atccgcagat aggccttatt
    27061 actgccgtag cctcccagaa attcataaag ttctaaacca taaggtgtca aacaggtggc
    27121 cacccacgac aaagccaggg ttaaaccaga ccgcataatg acacctttag gtaaaatatt
    27181 gatctcttta ttagattgat ggggtagcca ataaaggata agcctaaaga aaaaccaggc
    27241 agtaaagatg gccagaccaa ttaaaaagcc tccatggagt gaggcccata gccagaaaaa
    27301 taaaggcaag acaacaattt tccaggttgg ccactcccgc ttacgtacta cctgagctaa
    27361 ccaaatggtt aaaactaaaa caaaaagaac agtaatttct tgaacccgca cgccaaaatg
    27421 cggcaaagaa gccgcaaggc caataagcaa taggagccaa aaaataaggg aattagcttt
    27481 attatctaac cagtgtttaa tagtctcttt agccagtaaa caccaaataa acatggtaag
    27541 aaaagcaaag agaaaattaa tcaaaagata acccccccaa ttataagtaa gataaatcaa
    27601 aacattagat aaccactcgt ggtctaccca agattgaccg gcgagcggaa aaagatgatg
    27661 ctctagatgc ggcacagcat gattttgcca aatctcttgc cctacggcat aatgccagcc
    27721 aaaatcaggg tcaagatagc caaaactatt atgcaaaact aagccaagac cgaagaagaa
    27781 taataaaatt attaaccacc ggctaaatga gaaccgttta gtagtttttt ggggcataat
    27841 tttgggttat aaaaattact attattatac taagtagtag attaattgac aagctcttta
    27901 tactctgtta tattggctaa acaaacttaa ttcaatctat atggcacaca aaaaagcggg
    27961 aggttccacc tcactaggac gtgattctca aagtaaacga ttaggtatta aattacaaga
    28021 cggccaaaca gctaaggccg gtgcaattat tgtccgtcaa cgtggtacta aatatcgagc
    28081 tggtaaaaac gtagctgtcg gccatgatta taccctattt gctactaaaa acggtcaggt
    28141 taaaatgcag accaaaaaaa tacgtcgttt cgacaatcag cttaaagtga ctaaaattgt
    28201 ccatgttgaa taaataccaa aatataaaat accacctatt aaggtggtat ttttgtttat
    28261 tcattattag acttaaattt ctccaataaa aactccctgg ctttctccat ttgcggatca
    28321 cgattagcat taaagtcttc ctcagtaaaa tcaacttcaa tattgggagt aataccctta
    28381 tcattaatgg aggtaccact tggtgttaac catttggcag tagttatttt aaccattgaa
    28441 ccatctggta agtcattgac catttgtact gatcccttac cataggtagt cttaccaata
    28501 gttgttgcct ggccataatc cattaaagca ccggccacaa tctcagaggc tgaagcactg
    28561 ccttgattaa ttaaaactac ggtcggtaaa ttacttagtg gagcaaggcc acgagccagg
    28621 tgctggcgtg gcggcttagt agccgattgt tcactaacaa ctactccact ttctatccaa
    28681 taactagcaa tggctacagc ggtatctaga tagccgccag ggttattacg caaatcgaga
    28741 ataagtccct tgggtttttt aaccttaatg tcttcaacgg ctgtggcaaa aagtgcctcg
    28801 gtatcggcat taaaattact aatagtaata gtataaatac cggtcgctga atcatacgaa
    28861 taatcaagac ttttaactac aatattatca cgcacaatag taatatcctg cagtttagca
    28921 tcaccccggc caatagttag ggttactgat gtccccttgg gaccgcgaat ttttttaaca
    28981 gcctcatcaa cagtcagacc ggccgtagat tgttgatcaa tagcataaac tttatcccct
    29041 gctctaagac cagcacgctc agctggtgta tcagctaaag gagcaataat agtaataaca
    29101 tcattgcgca aaccaacctc agccccaata ccactaaaac taccagctaa atcctcgtta
    29161 aacatcttgg catcctctgg atccataaaa accgtataag gatctttggt tgaaccaact
    29221 aaacccttaa gggctccata aaacatttgt ttatctgtta gctggtcatg atccacataa
    29281 ttactcttaa tcatatccca ggtctgccaa aataaatcaa aattaagatc agaagataaa
    29341 cgtcctcctg tgtcagaatt atataatcca gtaacttttc ccaaaaatac tatctgaggg
    29401 tcagataaac ggcggactat ttgtgctttc tcgtcataaa gaatcccact ccaaaaaccg
    29461 atagctagag ctataaggaa tagaaaaaga actaataacc agcgccattt gcgccgtgag
    29521 gtagacgatg aactcatata taaatatttt tagttttatc tagtgtagca caagttgaaa
    29581 gaatcgacaa agtagtctat gatagtaatt aataaataat taaacctatt attatgagta
    29641 agtttataat tcaaggccaa gcaccgctta aaggacagat taaggttaat ggggctaaaa
    29701 attcagccct caaaattatc cctgctagtc tactctccga agaaagtatt accattaata
    29761 atttgccaga tattgaagat attaaccgct ctctagaaat tattaaggcc ataggcggac
    29821 aaataaaccg ccaagaccct catcaatgtt ccatttgcac taaaaatatc cacagtacca
    29881 gcatcccttc agagctagct aataaattta gggcctcaat tatgtttgtc ggtcccatct
    29941 tggctcgctc cggcgaagtt agcttccctc atcctggtgg ttgtgttatt ggagcaggca
    30001 taagaccaat tgacttattt ttggaaggtt ttgctgctct tggggcgact attcatatta
    30061 ccaataatta ttatcatcta agcgctaaaa aacttaaagg agccgaatac ttttttacaa
    30121 cagtaagtgt taccggcaca gaagctctaa tgatgaccgc caccttggct caaggacaaa
    30181 ctaccttaaa aaattgtgct atggaaccag aaattgtggc tctggccgat tatctcaatg
    30241 cccaaggagc taaaatttct ggagctggta ctccgaccat aacaattgag ggcacaaact
    30301 atattagggg tggtcatttc acggttattc ctgaccgcat tgaagctggt acctttgcca
    30361 tcttagcggc cgtaagccaa gcagaactat taattactaa ttgtaaccct gaccatatag
    30421 ctattttact aaatatcttt cataaaatag gtgtccattt tgaacaaggc caagactggc
    30481 ttaaaatact acctagtcct aaaattaccg cctataatat taaaacccat gaatatccag
    30541 gctttcctac tgaccttcaa tcgccctaca ctgtcttaat gacccaagct cacggcttaa
    30601 gcattattca tgagactatt tatgaccgcc gtcttctctt tactgatatg cttactcaaa
    30661 tgggagccaa catcattatg tgtgaccccc atcgagtagt agttaacggg cccacccccc
    30721 tccatggccg caaactaact tctcctgatc ttcgggctgg tatggccatg gtcattgccg
    30781 ccttaattgc cgacggaaca acagaaatag ataatattta ccagattgaa cgtggctatg
    30841 aagctatagc tgaacgactg caaactattg gtgccaatat tcataaaatt gactaatatg
    30901 tcgcccagac aacgctggtg gctgtctaaa atatttatcc tcctcttttt tcttattacc
    30961 ccgcccattc tcatctatgc caatggttac cgctttaatt ggcattcctg gaaattagaa
    31021 cgaactggac tattagaaat taaaagcgaa cccagtaatg cctcagtaaa tattaacgaa
    31081 cttaaggcct ggtgggggaa acccaaaaaa atagttactc cagttaaatt aaaaagtcta
    31141 agacccggca attataatgt aagtctaagt ttacctaatt attatgagtg gcataaaact
    31201 ttaagtgttt ggccagccca gacaactata gttaaagata tccgcctatt taaagaatcc
    31261 aaaccacaac tattggctac tctcacaccc tctactaccc tcactagcgc cacattagat
    31321 aatcatcaac ttaatttagc aactaaaaat ctaactacca caacaatcag cgctttaata
    31381 gatcaagaaa aactagctaa aaattggcta gatactacct caattaaatt agcctgcccc
    31441 ttacccaacg gtaaaattgc ctttagtaat caatttgaat tatggctagc caatcctgat
    31501 gaagcaaaaa ccgaactttt aggtcgcttt agccaaccga ttactcagct gatctgtcta
    31561 cccaatacgt ctaattattt aatcttcggt caagatagtg gttggaaaat tatagaagta
    31621 aataatgacc agcctcatct tatgtgggag attgctcata ccgaccatct aggagcaggc
    31681 agtcttgata aaaaagctaa tactttatat ttctacgccc aaattggttc aaccaccggc
    31741 ttattttctt tagaattata aagataatgt cctaataaat ttctttagct caggaccaat
    31801 ttcttcattt ttcaaaccta gttgaatagt ggccttaatg aaacttagtt tatcaccagt
    31861 gtcaagccaa gtgccattga actcttgccc ataaatagga cggtcatggg ccaacatttg
    31921 ctgaaaagcg tcggccaacc gtaattcacc gtttggtcca ggtttcatgg tggtaagcca
    31981 agataaaact tccggcgtaa ggacataaat gcccactgct actaaattag agggcgcttc
    32041 gctaggcgat ggtttttcta taaaacgttt aatttcatag gttcgctcac ctacggccac
    32101 tccatcaatc actccaaatt tactaacctc gttgcgtggt actgccgaaa gaccaataac
    32161 tggatcacca tatttagcaa aagtttttaa aatttggcta gctggaccct ctttggcatc
    32221 gtatagagta tcaccaaaca taactagcac tggctcatca tatattagat gagccgcaca
    32281 agccaaggcg tggccatcac ctaggggtag gggctgacga acataagaaa atttagctaa
    32341 ttcggaaata cgccgtacct cttttactaa ttctaattta tttttctcta ctaaaatctt
    32401 atccagctca aaagaataat caaaatgatc ttcaatggct cgttttcctc gcccagtaac
    32461 aaaaatgatc tctttaatac cggcagaaac tgcatcttca acaatatatt gaatagccgg
    32521 tttatctacg atgggcaaca tttccttggg ttgagcctta gtggccggca aaaacctagt
    32581 accaaaaccg gctactggca aaatagcttt agtaatttgt cgcatatttt ttagatattt
    32641 ttaccgcgag gtacatgaga tttacctccc ggtccccatt taagaagata tttaaaccaa
    32701 gagagcacat gttgacgccc ggcccagctt cttaatatct tataccacgg catctgagca
    32761 ctcgctcgtt gatgatcatg gagaatagct acttcaggat agtaaaccac ctgccagcca
    32821 tgacgccaca ttttccgact taagtcggtg tcatcaaaat ataatcggta acgttcatca
    32881 aaataaccaa cctggcgcat cgccgttgtc cgaataaata aaaaagaccc tagtagccaa
    32941 tctaccggac aaaattgatt taaatcaaca tcagccaaga gaaaacggcg caattctttc
    33001 tgagcccatg agaacttagc tagccgggga gagcgacggc aaaaaggagt ataaaggcga
    33061 tgccagcgat aacaagattc ctgcctgtca ccatcaggat tatattgccg tggtcccacc
    33121 atcccgactt gaggatgatg ctccataaaa ttataaagct cccaaataga atttggctct
    33181 accgcagtat ctggattcat aaccacaaag tatttaccgc tggcttgacg caaggctaga
    33241 ttattaccag ccgcataacc aatattgtta ctagggatat aatgaacctc tggccaatga
    33301 gtcgctaact cctgggccaa atcagcttca gtagcattat ctactacaaa aatttcatat
    33361 tctaaatcat gccactcagt ctttctaagt gactctaaag agcgataaat taccgcctgg
    33421 ctatgataac taacaatgac aatactaata tccatagcta ccaaataata atcagtttaa
    33481 taatctgcca ataaagatta agaccccagt tacctaccct aactaaaggg gaatcaactt
    33541 cttgaaacaa aatacgacca ctaaaagtct taataacaag atggtctctc acctggcgta
    33601 aacgttgact tcgaattcgc gctttgaaaa ggctcggcca atgaagtgga ttgcaaaaat
    33661 gccaccaggc tttcagttta taaattaatc ggccgctaca gcaataaacg acaactaaaa
    33721 caatttcgct taataataaa gctggcagta aaacaagtaa ggtcaagaaa tgataatttt
    33781 tccaggctga aagtaaacga ttacggtcta gccaataata tttactgacg cttcggctaa
    33841 attcatactt atgataaacc atggaataag ccgctaatcg acactcccag ccggcgagcc
    33901 ataaacgcca ccctaaatct tggtcctcat tatacatcca gaaagtttca tcaaaaagcc
    33961 caacctgccg gagagctgac agacgaaata ataccgaagc gccggaagga taagaaatag
    34021 gctggccatt aagttcatct aaattgagtt cagctactct tttcttatag ccattacaat
    34081 aaccatagcc aagaaagtgg gttacattgc ctaaactatt gactaaatct ttatctggcc
    34141 accattttag aagtgattga gcacctccaa ttttaggatg actaatcatt aattttacta
    34201 attctatcaa gctatctggt tccactatag tgtccatatt aagtacccaa ccaaaatcat
    34261 acccccaaga cattgctaaa gctaaggcat cattattgcc tttggcaaat ccatcattat
    34321 tggtattgcg gataatataa gcggtcggcg ccatagtttg cagggtggta taacttgcct
    34381 catcagattg attatccaca ataaaggttg ttgcttcaat ttgccagtca ggggtaatta
    34441 gattaagtcg cgataaactc tcccagcaat cagctaaata tttattagcg tagtctttat
    34501 aattaataat aataatggct attttgtaat tcatcataga tttaagtttt agccggccga
    34561 tgccagttta atttttctcc taagacagcc ataaaaatac caatgggcca agctaatttt
    34621 aagagtcgtg actgccaccg tggttcccat ttagcaaaat atttaatcat actttcgcga
    34681 aaataagtct gtgcctgacc acgacccact tgcttaaaac tttgacccac ataatctaca
    34741 caggtagcaa ctggagtata ccatacttgg ccaccattta atttaacctg ccggcaataa
    34801 tctacttctt caaaccataa aaaataccgc tcatcaaaaa gaggcacccg accctcacgg
    34861 tcagccctaa tccaaaaaaa gccaccccgc accgaatcaa ctggcgcggc ctgagtgtaa
    34921 tcaaaatcag ttcttaaata atgatccaga acatgaggaa aaagatgggg caattttaaa
    34981 acaatcgcca actggtccca aatagtcgga aaacggcgaa catgtaataa agtttcaccg
    35041 ttttctgtta ctaaatgaca accagtcact gtggcttgcg gattagcatc acaccacgcc
    35101 aacatattgg ctaaggtatc cggccgaact aacatatcag gattaagaag taaaacataa
    35161 cgagccctga tctctcttat ggcctgatta ttcgctctag caaacccacg attatctgga
    35221 ttggcaatca gatgaacctg aggaaaatca tgtttaatca tctccactgt gccatcacct
    35281 gaattattat ccactaccca gacttcgaaa ttaaaattac cttgactagc taaaagttct
    35341 ttaagattag ccgccagttt atctttaact ttccacgaaa caatgacaat agctaaatct
    35401 aacataaatt attgcttaat aacgccattt ttctagcgat aatggattaa ttttaatatt
    35461 taaagctcgt cgccgggcaa taatagcagc cctttcctta aaaattgctt tccagacaga
    35521 taaagtaaaa ggctccagca ttaccgccac agccaatctt tttaattgat agtttacttc
    35581 tttcaatcga gtggatagct tcttaggtaa agaccaatat ttcaaaaaaa taactaactg
    35641 gttatgatag gaccaccgcc taattagttt actagattgg cgacgttctt tccactgact
    35701 tttccaccca cccctagctt tgacggcagt acggtcatga taaattacgg cctcaggcac
    35761 tagcacaatg tcccagttgg ccaaacgcaa acgataagac aaatcacaat cttccttgta
    35821 cataaacatt agctcatcaa aatattctaa atattgacca ttatcaaaag caaccgacct
    35881 taaagctgac agccggagta aaacaccagc ccccgtaaaa ccaaaaacag aatgagtata
    35941 gtttaattct ctaccactaa gctgaccctg gccaatatca taaaaagatc cgcccttagt
    36001 tagggctaat cctagactat ctatctcctg agttaaatgt tgaccctgaa aatcccaccg
    36061 taaaatttta ggcatagctg cacctacctc gctatgttca tcaagataat ttactagaca
    36121 ctcaataacc tttggctgta gtaaagtatc aggattaata actaaaaagt aatcagcctc
    36181 ctcttccatc gctagtttaa acagtcgatt atatccttta gcaaacccca aattagcccc
    36241 aaagggataa tagcggatag ctggataatt cttaataatt aattcattag gattatcatc
    36301 aagagatggt gtattgtctc cggcaataat ttctatatct gtaaaagttt gctgtctcaa
    36361 actatctaaa aatttaggta aatatttagc tgtggactgg ccatagccca aaaaagcaac
    36421 ataaactcgt gaaccccggg ccatattatt atggatgacg ataaaaggca accatctcgt
    36481 catcaaaata atttttatta tttaaatatt tttcggtccg ccgaataatt ttttccataa
    36541 tagctacggc tcgagctgga tactgtccca ccgctgtttc gtcagaaagc aataaaccgt
    36601 cagcatcatc tagaacagca ttggcaatat cactgacttc tgctctagtc ggccggggat
    36661 ggtcgatcat tgaggtcatc atttgagtag caataatggc tggctggcca tgccaatgag
    36721 catggcgaat taaattcttc tgaataatag gtaaatcttc taggggtact tcaataccaa
    36781 ggtctcctcg agcaaccata ataccatctg attctttaat aatttcatcg atgttttcta
    36841 gggctacagc gcgctcaatc ttagcaataa ttttaatagg acggccagcg ataattttcc
    36901 gtagcttccg caattcagtc gcactctgga caaaagacag agcaatataa tctatacccc
    36961 cacacttaag agccgcttcc acatcagtaa tatctttagg agttaatcca ccacgcctta
    37021 aacgagtatt aggcaaatta atgcctttat gagataacaa tagaccacct cgctctacac
    37081 gagcatagat tttcttatct ttaatactaa ccactttaag ttctaattca ccgttagata
    37141 aaaaaaacaa atccccgccc ttgagatctt tgtataaatc agggtcatca atctgaattt
    37201 cccgattagc tacactggag gggccagccg taaaagtata aacttctcca tcaatgagct
    37261 caatttcttg gtccagctga ccaattctta aacgtggccc ctgtaaatcc tgcataatta
    37321 aagcttgacg gccagtttcc tgtttaatct ttttcaaatt cttttttaaa tcaaccaact
    37381 gcttaatatc tgcatgagaa aaattaagcc gtacaatatc ggcaccattg cgaataagac
    37441 ttttaagaac attatagtcc caagaacttg ggccaatagt cgcaataatt cgggtattca
    37501 taaataaacc tttgtcccta ttagataatg agcttatttt gcaggcggtc tctagctaaa
    37561 atttgagcat ctaggagact atcaaaagta ccagcatcaa tccaagctcc ccgcactaaa
    37621 cttacctcta gctcatgacg tgctaaatac cagttatgta aatcaacgat ttccaattca
    37681 ccacgtacac tgggcttcag ttttttagcc acatttataa cccgatggtc ataaatatat
    37741 aaaccagtaa tggctaaatt gctcttaggc tgaagtggct tttcttcaat agacaaagct
    37801 tccattttat tattaacttc cacgactcca aaacgttccg ggtctttaac tttcttagca
    37861 aaaattttag caccaccctt aaaattatta atatcatcag taaaatcatc ctcaaagata
    37921 ttatcaccta aaatcatagt aacgctctct tcatcaataa aattttcccc aataataaag
    37981 gcctcagcta aaccccgtgg cttatcttga atctcataag ttaatttaac cccaaattgc
    38041 cgaccactgc ccaataaatt aagataatca ccggctcttt cgggggaaac gataattaaa
    38101 atttccttaa ttccagcttt aattaaagtg ttaagcggat aataaatcat tggccgatta
    38161 taaattggca atagttgttt agaagttact ttagtgcagg gtcttagacg agtagcactc
    38221 ccgccagata aaataatacc acgcataaaa ttaattttta gataattgac ttaaatattc
    38281 ggccagagcc tcataatagg gtcgcagtcc cgacagttta gtcattaaca atgtagaata
    38341 ctgtggccga gctgctggtc gaggcaaatc agcactacta attgctttga tgggtaaagt
    38401 taaatgggct aattcaccta atttttgtaa agcaccgtac cacgtagtcg gttcactatt
    38461 tactatatga taaatcccgc tagctgggcg ggaagctaat aattgccaag tagcttgagc
    38521 taaatctgga gtataagtaa aacagcttac ttcggcatct acacctttaa gctcagggga
    38581 agtagcggct agttgaaaca taatatcaaa aaaactaggt ttagcattag gactaagacc
    38641 acgcggtcca aataatttag aagtccgaat aagataatag cttaaacctt gttctccttc
    38701 agcaattagg gattcctcac cagctcgttt actcctacca tattcgttaa taggttgagg
    38761 ctcatcagct tcgtggtaac ccccaggttt atcaccacca aatacataat cactagaata
    38821 atgaactaaa gttaaacccc gctcccgaca ccaataagct aaattagcta ctagctgatg
    38881 atttaaaata tcggccatgg tttttcccgg ttcagtctcg gcagcatcaa cagcgttata
    38941 ggcggcggca ttaattacta tctcaaggtt agaaagtgaa tctagtctgg cttgagtaag
    39001 tggccaatta cttaaatcaa catcctcacg atcccaacta ataatttcat agccccaaga
    39061 tgagccactt tgtgttagtt gagtacctaa attaccctgt ccacccaaaa ctaaaacctt
    39121 catattctcg ccttaagtgg acgccaccag gcttcatgtg ttttatacca ttctactgtg
    39181 tctttaagac cagaagcaaa atcaatccga ggctcccagc ctaattcttc tttggcttta
    39241 tgaaaatcta tactataacg ccagtcatgc cctggccggt cagttaccaa ttcaatataa
    39301 gattcatctt tacccaaaag tctaataatt tctttagcta actctatatt gctaacctca
    39361 tgattgccac ccagacaata agtctctcca atacggccat gcagtaaaat agcttctagc
    39421 ccggcattat gatcatcaac gtgaatccag tcacgaatat tagtaccttg accatagagg
    39481 ggtactttgc gatcttcaat taagttagta ataaataaag gaataaattt ttctggatat
    39541 tgataaggcc cataattatt agaacaatta gaaatagtga ttggcaaatt ataagtacga
    39601 tagtaagctc tcgttaaatg gtcagccgct gccttagaag cactataagg actactagga
    39661 cgataaggag ataattcatt aaatttagga tcattaggac ctaaatcccc ataaacttca
    39721 tcggtggaca cttgatgaaa tcttactccg ccgtgctggc gcgcagcttc tagcaaatta
    39781 gccgtaccct cagtattggt cttcacaaaa accaaaccat tattaataga acgatccacg
    39841 tgagtttcgg cagcaaaatt cactatgcag tctacttgac ccactaattc atcaactact
    39901 tcataagaag caatatcacc cttaacaaat cgataattct tctggtcagc gacgtccgcc
    39961 aaattagcta aattgccagc ataagtaaga aggtctaaat taataatctc atcagctgga
    40021 tgctttttta accagtaatg aatgaagttg gagccaataa atcctgcccc acccgttact
    40081 aataatttca taaaaactaa tttaaaaaat ggctaattta tttattatct tgacccatca
    40141 ttaaaaaaga cattatttgc tcccaacgaa tttcatctac ctcctcaact ttattagtcc
    40201 cacgatataa tttatctggt aaattaatag accaggcatc atggtcacca atggctttgt
    40261 agccgtgaac tactcccggc ggcacaatta tcattacggg attactttga cctactattt
    40321 ctttcatata ttgacctttg gtagagctat tttctcgccg atcccataca tgaaactcaa
    40381 aatcacccgg ccccacaaac acaaaacaat ctgattgata cttatgctca tgcggtccac
    40441 gggctacccc gggatgagta acactaacat aacccatgac tggttggtaa tgatcttcat
    40501 catcacgata aatctctata agccaacctc gctcatcttc atgcggcgta agatgcttaa
    40561 taacaacacc ttgaatcata aaccacaaaa attattattt attaaccaaa agcatttcca
    40621 gaatagcact ggttgcccaa aaaagcaacc acaaattaag attaatatat ggctggacaa
    40681 aaccaggtaa gatagcctca gaaaccagaa aaagtaccag accaactaaa agccaattat
    40741 agaaaaaatc attcaattta agacagcttt gccacttaaa tttcatatta tcttgttaat
    40801 tttaaccata aaaactgtag tttatgctct atggcttgcc ataaattaga accgattaat
    40861 ttaacctgaa tttcttcaat agtcaagggc gaagaagtag atagctgtcc accgctaacc
    40921 gcaataataa actggtagcc atcattaggt tgccaaaaag cctccttaag cggtaaaacg
    40981 gctgaagcct tacaaatttg ctcattacaa cttggcattt cataattagc ggctacaaaa
    41041 ttaatatctg ggtccttatc taaaaaacga tctacccgac gtggataggg attaaatact
    41101 tccgacgaca aggaaaaaac accattacca ctaaactgaa tatccccgcc ccgccccttt
    41161 atctcagtaa caggatcctc agtaattata tgatattgtt tataagtact atcaatagtt
    41221 agagactggc tgccgacatt aatagtctcg caactagttg gttgaatagt ttgagctgta
    41281 agttcaggaa catcagtcca catctgccaa gaaaaatctg ggctaaagac aggccatacc
    41341 tgattttgcc aactaaaaat agattgcttg gtggctaaag taaataaaat tttagagcta
    41401 gccttaattt ctatgttata aaccgcagct accttagaag gaataattat ttcttggctt
    41461 acctgttctt gctctaaaga aaaacgcgac gattgtatcc tcgtgctgtc aggaccataa
    41521 atattaactt ctatctctct ttcatcggct gtaagcggct taacactgtc gctaactgcc
    41581 gttaaacgcc aagccaaact ttcccctggc ccaacataaa caattaaatg ctgactacca
    41641 cgccacttac caggcaatgt ctgccaatta gccttagcct gataattgtc taatatcggc
    41701 gttatggcta atggccagga aaaattataa agagccaact gtgaagctgg tgggggatta
    41761 ttaataaagt cttgccagct ggaatatact ggttttctct gccaaagaca ataagatcca
    41821 ttcattaaag aaggccaatg taaattatta aggacaggat tattaagagg cttggtaata
    41881 tattgccaat gagcccgatc taccaatacc cctacctcca agggaacatc actagatttc
    41941 cgccatttaa ttgttatctc tgcttgatta aaccgccgcg tcggccttac ataaaaatag
    42001 cctggctcac caatcattac tagtccccca ctagaagtag cccatctttc aggaggtcca
    42061 aatttatgaa ggaaataagt accagaccaa tcattaaccc tgtaaattgc tactcctgtt
    42121 ggccttatgg ctaaatataa taagacaagc cagaccacaa ttagacctaa ccaaagaatt
    42181 ttctgccatg tctcaagctg ccgccatcct ttaaacataa tatcagatta aataaccttt
    42241 aagattaaag atagttaaac cataaactat aaatcacttc caatttatgt tattatagca
    42301 caatatgatg agaagaaaat taagctggtg gctattatgg ctactcgcgg taatccttat
    42361 tatagtcatt ggttattata accgacacct cgtagtcact aattataaaa tcagcgaccc
    42421 gcgctggtca gctgagctaa atggctggcg tttagtccag ctagcagatc tccataacca
    42481 agctactgac ggaccaggca ataataaaat aataaacgcc gtccaaaaac ttcagcccaa
    42541 tattattgtc attactggtg atttatttga tggtgataaa accaatattc ctaacaacct
    42601 tagactagta aaagatttat cctctatcgc cccagtttat atagtccttg gcaatcatga
    42661 attcgccaac gatgattata taaaactaat ccaattggtg gaagaggccg gagctcaagt
    42721 cattcgcaat gaagtaatta ctattaatga caaaggcata ccaataacta tcatcggctt
    42781 agatgatcca gcggcctggg gctggccgct tagctataaa actcaaacgc aactaacagc
    42841 tcaaacatta aataaaataa tgacctcgcc tgattttaac tctacctctc cccgccttat
    42901 cttagcccac cggccagaat tatggcctca atatctatta gccaaccccc tcattattct
    42961 ttctggccac acccatggag gtcaaattcg tcttccctta attggagcca tctatgtgcc
    43021 taatcaacca ttgctagccc aatatgatga gggtattttt gctatagcta atagctacct
    43081 cattattagc cgtggccttg gcaatagctt tattaattgc cgttttaata attatccaga
    43141 aatagttact gttgatttct ttcatgaatg acgaatacgg tccattaatc ttaaaaggtc
    43201 atcgtcatta ttaaacttaa ttctaatctc gccaccgtgc tcttcacgca caatcttaac
    43261 tttagcttgc aaaaattcct gcagaagaaa ctctcggccg cggtcattat tcttaggaat
    43321 attatctgag gaagatgcca aaccctgtaa atgtcgaacg gatttagttg tctcagcgac
    43381 tgttatacgt ttatttttag aggccatgcg agaaaaagcc tcaatctgct tacttggttc
    43441 cagtccggcc aaaactttag cctgacccac actaatttgc ccagctcgca gagcttcctt
    43501 gaccttagat ggtaaattta gaagccgcaa tatattacta atagtggaac ggcccttgcc
    43561 caaactttta gctagttcat cttgagttaa atgaaactca tctagtaact gacgataggc
    43621 cctagcctct tctaggggat taagattttc tcgctgaata ttttcaatta aagctaaaat
    43681 taatttttct tcttcgtcgg ctgaacggat aataactggc acttgtttaa gaccagccag
    43741 tttagcggct ttaagacgac gttctccggc aataagttca taacccacag cagttttagc
    43801 gacaattagg ggctggagaa taccacattt tttaatagaa ttagctaact cttgaagact
    43861 ctcggctgaa aaattctctc gcggttgccg gggattaaat tcaattaaat caacggctaa
    43921 ataagtaatc cgctcactat ctattggagt agctgctgga ttactagtta aaagggattc
    43981 taaacctcga cctaatcctg atgatgacat atggcaatta ttattattat tttagggcta
    44041 aaatttcttt agccaaagcc tcataagccc gactgccggt tgagtgctta tcataatcaa
    44101 caatcgaccg accataacta ggggcttcag ctagacggac attgcgagga ataatggttt
    44161 taaaaacgtg attaggaaaa aactgttcta cctccttaag caccgcccgc gctaaacggt
    44221 tacgctgatc atacatagtt actactgccc ccaaaatacc caaattaggc ttaaggtggg
    44281 cctgaatcaa gccaattgta tctaacaatt gctttaagcc ttctagggca taatattctg
    44341 cttgcacggg aattaaaaga tgtgtcgccg ctactaaact attaatagtc aataacccta
    44401 gagacggtgg accgtcaata ataatataat caaaatcagc ttgcactgag tctagagcct
    44461 gagctagctt aaattcacgt tcctctagat taaccagctc aacactagct ccagctaaat
    44521 taatggtgga cggagccagt aaataaccat tgggtgaatt aacaataatg tcggccatgg
    44581 atcgctgacc aatcaaagcc tcataaatac ctgatgataa ctcagctggt aaaaacccag
    44641 agccagaggt agcattaccc tgaggatcaa tatctataag taaaacccga cggcccattt
    44701 tagctaaata ggcagctagg ttaaccgcag tggtagtttt acctactcca cctttttgat
    44761 tgacaatggc aatgatttta gacatggggg attattactt agatattata gcgaattgct
    44821 gtttttttga caattttatt tctctcgcct ataataattg gccgagcctc ggtggcggaa
    44881 ctggtagacg cgcacgactc aaaatcgtgt gagagcaatc tcatgagagt tcaagtctct
    44941 cccgaggtac aacaagcacc ttaaaaaata agaggaggca aaaatggaag aaacaaagta
    45001 tttctatcca atcattacat ttactaactg gctagcaccc atcctggaat ggtgcagcct
    45061 acatcactac caacctcagg accccaatag gttggccaga attataataa atagttattt
    45121 ggtggccgaa aatgaaaccg caccatctaa tatagaaaaa ataactggcc tcatcaagtt
    45181 tgcggaatca tttatcacca aagaaaagct gttactatgt ctaggcgaaa ttggattttc
    45241 tactcaatac ccggaagtgg tctgggaaat aaaaaaatcc aataactggg ccgactgctt
    45301 ggcggcagag gctaaacttc tggaaagact gagtcctact actcctggtg catggcttat
    45361 gtctaaagat gagctgaatc aattctgggt gtggtggcag tggatcgctg accaaacctg
    45421 gcattgggtc aagaccccag actttcccct ttctcatttt ctcatccaag aacatctaag
    45481 ctgggcgcaa aacatcatta ctagtttcag aagaaattac tgcctactct cagacagctt
    45541 agaagaaaat gagaccaata taaggaattg gttacaaagg gtagcaccta atgctgacaa
    45601 tattactatt gaattggcca ttaaaaaatt aatgggcttg agagctccgc tccgggctgg
    45661 atgggataag gcgctccaaa cagccatgaa agaaactgcg gatcaacaga gcttttggag
    45721 aaaaaactta gaccccactc cctttgctca aatgccagat ggtttgagct taatcggtga
    45781 gagtctacaa caaattatta cataacaaca atctagccgt tagttattta gctaacggct
    45841 ttttattctt gcctagcctc atctgcgccc ggcgccactg ggcaatagcc tgatgattac
    45901 cggataataa aatatctggg acagcataat cccgaccgtt taaggtcaaa acttctggct
    45961 tagtatattg aggatactct aattgaccaa cgtggctatg cgattcttct agaagactgg
    46021 ccacattacc gactacccct ggtaataaac gggcaataga atcaataatg accatcgccg
    46081 ccaactctcc accggttaaa atatagtcac caatagaaat ttgttcatca actaaataag
    46141 ctactctttc atcaactcct tcataacggc cacaaattaa aactacttca tctaactgac
    46201 tataacggcg ggctttggcc tgggtccatt tattcccccg gggagacaat aaaataacct
    46261 tgcgtttagc ataattagtt ttaatatctt ctaatgccct agcaataggt tctacccgca
    46321 ttaccatgcc tggacccccg ccataaggag tatcgtccac tcgccgctgt ttatttaatg
    46381 accaattatg taaatcatgg atttttactt gaaaatagcc ctgttcttga gcccggccca
    46441 aaatagattg gtctaaataa ctaaaagctt cgggcaaaac agaaataact tggaataaca
    46501 tagaaattat cttattttgc ttattatatc ataaaaacaa aaagaccgga aaaattccgg
    46561 cctttttgct atcctcacaa aaaattttag atagatagat tctctaccga agaagtgtca
    46621 attttttctt cggtgggacg ctgtggacga ggttcacgac gtggacgctc tccagcatta
    46681 ggatcataaa tttttagatt gacccgggca ttgttcttag caccgacaat ctttaataaa
    46741 gtacggatgg cctgcgccgt ttgaccacga cgaccgataa cataacccat gtcggccgga
    46801 ttaattttta aggtcaaaag gacgcccatc tcgtcaacgg ttcgctcgac tacaacatct
    46861 tcgggctggc tgacgatggc cttaacaaca tactctaaaa actctttgtc tttaagttcc
    46921 atagttttta ctttaactta aaattaggct aaattaccta actaatacta atctaacata
    46981 gctaaaatat tatgtcaatc atcgctaaac tagacttatt tgcggtatac aaaaaagcgg
    47041 taaagtaaaa aattccaaac cgtagataga gcaatattag ccagtcgcat taacagataa
    47101 ttaaaaccta aataaccatt aaaaaaccac atccaaaaaa tggcaatacc ataattaaca
    47161 atggtcaaaa ttatatagcg agtcgcctgg cgagtaaaag taccactgga accaaatacc
    47221 caatatttat taagtaaaaa aatataggct cccatgccta tttggttaat tattactgct
    47281 accaccggtg ttaaaccaac aatagtcttt aataaataaa gggtagttaa atctaatact
    47341 accccactac agccgactat aaaatagcga agaaactgag gggacttaaa ttggctaaga
    47401 aaaaaatggt ataacctact aagtggccga gaagactgca tattaaaagc tagctaagcc
    47461 caaacacttg taaatgaagc ttcttggctg actgatcatc taactcttta tacaccgccc
    47521 ggatagtata acgacaacga ccattaggca aagaaaattc atcaataacc gctaaactcc
    47581 ataggctttc ccgaccaagc ttatcctcaa tatcttgaag ctttatacct aattctcgac
    47641 ggtcatctgc ctctagatta gcatccaggg tgattaattt ttgagttatc tctacggttg
    47701 gcgctggcat tttatgctcc tgtaaataac ttattaactg ttccaggtca atctcggccg
    47761 tagccataat cttatcatta taatgctctt taagctgggc taaaaatcca ataggctctt
    47821 ccccaattat tatttcccag gcagcgtagg ggttagcaat ggctggtata tttttagccg
    47881 gatagaaata aactcgttcc actccactat aagccaaaaa gccgctaacg gtttgacgta
    47941 aatcgtcaat tactcgatca ccgccaccta cgagccaacc aagatgttca gcttcttggt
    48001 aactattctc caccttacca aacaccttgc ctaactcaaa aatctttaac tccggtactt
    48061 gttctttagc atattcatga gcctgcttaa ttaagctaac aataatggtc tgccgcagag
    48121 ctggataatc ttcattgatg gcgttttgta ctgtggctat tgcccaaggc aaataattag
    48181 ttaaagcatt atcctctagg cccaccatag tccaggacaa aacctcatcc cacccctgga
    48241 aagccagcca atcacgccat cgctctttag ctttagtcag tggcggggtt atatctaatg
    48301 tcgccggcag agcaggcaaa acagaaggaa gtttatcaaa acggtaaaga cgtaaaactt
    48361 cttccaccac atcctcagga agctctatgt cagtacggta actagggaca gtcacctccc
    48421 acggcaactg ggcatcatta actgtaaatt ctagctgact taacagctgc cgacattgag
    48481 ccaactcaat gtttatgccc cctaattcac ttacgagttg tggctcaaac ttaattgtct
    48541 ttacttcggt tttttcttta taatcttcca ccacctgact ggctatttca cctcccgttt
    48601 gatctaaaag tagattaatt aataaattaa aagcataatc taaatctacg aggctcagat
    48661 ttttactcag tcgattacta gcttcagtaa ccacgtgaag cgctcggaca ttacgacgaa
    48721 ttagagctgg atcgtaaagt gccatttcag ctaagataat tttggttttc tcagtaatgg
    48781 gagcataatc ggtgccaata atacccgcca aagcaataat actatgacgg tcgtgaatga
    48841 tcagttcttc atcctttagt tttacagaag ttccgtcaag cgtagtgatc tcgcgagact
    48901 gatggttaat agaccaaacc gtgtcaccgt caagtaactc ataatccatt aaatgagagg
    48961 gatagccggt aagtagcatc acataattag acaaatcaac cggcaaatta atgctattta
    49021 tttcataaag agctagataa tcaacaagcc attgaggaga aggtttaatt gaaatattct
    49081 gaagcttaac agccttaaga cgacgaaccg ctgaagtttc tattctaatt ggcacctctg
    49141 tttccgctgg cgtcaattct gtagtgggag gcaaattaaa tggcaaatta aaataagccg
    49201 caatttctcg agtaagacca ataacggata aacaatcggg acgattttgc ctaatttcaa
    49261 aacccaataa aacatcaggc tgaccattat aattaaccat ctccaaactt tcctgcataa
    49321 agccggtcat agttaaagct tcaccaattt ccgcgggagt tgcggctaaa ctaggaatta
    49381 attgtttaat ttgactatat aatattttca tgatggtaag gtttatagcc taaatttccg
    49441 cccagcaggg tacggatatc agtaatatta taactagcca tagcccaacg atcgagtcct
    49501 aggccaaaag caaagccatg ccactcccgc ggatcaagac cagccattgt taaaactttt
    49561 ggatgaatca acccagcacc tcccatttct atccatccca ctcctttaca aaccgaacag
    49621 cccttacctc cgcaattaaa acactgcata tccggcccca ctcctggctc tacttcagga
    49681 taatacttat tgcggaagcg aatctttacc tctggaccat aaatagcttg aaagagggca
    49741 gtaaaagtgc caaataaatc ggacaaactt attttaggca ttactactac tccctggtaa
    49801 tgatgaaaaa taaaatgatt gcttttatta accgtctcat tacggtaaac tcgacccgga
    49861 cataccactt ttaggggggg agcataatta gccagcaccc tcgcctcaac tgatgaggtt
    49921 tgggtacgca gtaaaatatt aggctcttct aaataaaaag tatcctgcat atcccgagct
    49981 ggatgatcta agggcacatt aaggcgctgg aaacagaatt catcagtctc aatttcccgc
    50041 tcttccataa tactataacc taaactaata aaaagctcat tgagtttacg aatagtttgg
    50101 gtaattggat gtaaatggcc taatttcacc ggtacaagac ctaaagattg tgcttcagct
    50161 tgccaagttg cctcttcgct ggccttctta agttcagccg ctcgagtatc aaataaagct
    50221 tgggcttctt cttgaatccg ttgccataaa ggcccttgtt ctcgcttttc ttcaagcggt
    50281 aattgagcta aagcctttac ctgggcctta attatcccct ctcgacctaa atatttatgc
    50341 cataaagcag ccagttcagc ttcactgcca acctctttaa tttgagtact aagttcagtt
    50401 aatatttgat cacctaacat agattacatt acttcagggg ctttaatccc caaaagagcc
    50461 aaaccattaa tgattacttg tcggatagca gctcccaacc ttagccttcc catctcttcg
    50521 ctagagccaa taattcttac tttttggtaa taattattaa actcttgtgc taaatcaaat
    50581 aaatatttag ctataatggc tgggtccatg gcctgactag cctgactaac tatttcacta
    50641 aaacgagtca gggttaaagc gagggctttt tctaactcat taggccgagc tattaaagtt
    50701 gagcttccat tcccttttaa tttggcttgc cgttctaaac tcgctaaacg tactgccgca
    50761 tactgaatat aggcagcggt atagccatca aagctcaggg cctgagggat atcaaaaaca
    50821 atcggtcgag ccggacttac tttaaccatc tcaaatttta gcgcaccaac gactacttgc
    50881 caagcggtct cctgaatttg ctccttactc cactccggat gacgctcttt aacttcccgt
    50941 aattcttgag taagcaaggc ttcgcgtaag gcttcatagg tcaccatatt accttcacgc
    51001 gaggacataa ggccagttgg taatttaaca aaaccataag atagatgacg aattggctta
    51061 ttaaacccta atttggctaa aatagctgcc aactgtttaa aataaagact ctgacgttca
    51121 tcaatcacat accaggactc atcaatgtca taatctcgaa atttagcttg tgctaacggc
    51181 agatcagcta caggataaag agtggtccca tcacgacgta aaaatagtaa aactcctaat
    51241 ccttctgact ctaggttagc taaaatagcc ccctcatttt gttctaggat gcccttcgcc
    51301 agcatctcct gaaccagctt acgaccttcg tctataaaat cactctcaaa aaaggtctgg
    51361 tcaaagtgaa tatctagttc ctcatagatt ttctgaaaat aatcaaggct ccattgccgc
    51421 gtctcttgcc accgactata ttcgggttgc tctcgagcct ctagacgtct taagattagg
    51481 cttactaatt cttgtcctac tgggtcatta gctaattcct gagcggcttg ggtataaagc
    51541 tctcccaaat aatggccaag atttttatga ggtaattcat tttccggtat ctgatttaat
    51601 ttccacagaa ctttggccac atggatcccg agatcattta aataagaaac aggaattacc
    51661 ttatagccag aaaaattaaa aagacgagtt aaactatctc caagacataa attgcgtaaa
    51721 tggccaatat gataatcttt atgagtgtta acattagaat attcaatcat aatcttacga
    51781 ccggccccgc tatttataga gccatactga ctacccgctg attcaatctc gcctatcact
    51841 ttattaatta gaacctcacc ccttaaagta acattaagat aagggccaac tgcttgccac
    51901 cccccaatag ctggatgctc tttagtgctt aattgagtga ccaatttttc cgctaattca
    51961 cccggcaaat aacctaattt tttagctacc gcaaaacaag gccagcccaa atctcctaat
    52021 tccctctgtg ggggaattac taaatcagcc ggtgatgcca agggctccgg caattcatga
    52081 ttaattaact cagtaatctc cttagtaatt tcttggtacc aacccatata ttaactaaca
    52141 actaagcgcc aaaattgttt cttgcccttc tgcaataaga gtggctcaga actaagctca
    52201 accgtagctg tactgtcagt aataacctct ccgttcactt taataccacc ctgttcaatt
    52261 aaacggcgcg cttcactacg gctggggacc gccttaagat gaaccaataa atcaatcagt
    52321 ggccacgtag tagaagctaa agcaattagg ggaatatctt cgggaagatt tttttgctgg
    52381 actacagtaa taaaataatt ttctgctgta gttgcggctg caactccgtg atacatcgtc
    52441 acaatggttt tagctaaaac cattttaatg tcccgagggt taaccccctt ttttagggca
    52501 acttcatatt ccttaacttc ctttaaagga actcgggtaa tcaacaaaaa gtatttaata
    52561 attaatgaat cttgtaaccc catcgccttg gcatacatgg tttgagggtc atcaaccaag
    52621 ttaattgtat tgccccagct agaactcatt ttacgcccat cagtgccttc aattagttca
    52681 gtcattataa tatcttgcgg ctcttgacca taataagtct gaagagtccg gcctgctaaa
    52741 agattaaagc gttggtcagt acccccaagc tccacttgag agcgtactgc gacactatca
    52801 tagccctgca ttaaaggata aagcacctcc cttaacgaaa cccttgagcc ggcctctaga
    52861 cgtcgtcgga tattgtctcg agcaataaat tcagcgaccg aaaattgatc agcttgggtc
    52921 gcaatctccc gatagcctaa tttatccagc cattcactat tataatgaaa ctcacaccgc
    52981 tcaagatcaa gaatcttgcc agcttggtta aaatattctt tcatgttttt ttctacttgc
    53041 tccaaagtaa gcatcggccg ctcactctcc ttatctgacg tatccccaat aaccccagta
    53101 aaatcaccga caataaaaat agcttgatgc cctaattcct gaaaatcccg caatttaaga
    53161 agtggtaccg accgacctaa atgtatttta gtgctagtag ggtcaatacc caactttact
    53221 cgtaaagtct ggccacttaa tagcttctct cgtaattttt ctttaactat aacctcatta
    53281 acccctcgcg ttaggagttc atcaataagt ttaggattag tattaaccat ataattaacg
    53341 gccaactaaa ggacaagagc gtttattaag ctccataatt aagttctcta gtagctcatg
    53401 ccgagctagc ggctcgtcag tcactttgtc tattttaata attatcttaa attttagacc
    53461 ggctggcgtt aattcttcaa aataaacttg gggcggacta actttttttt gatacttctt
    53521 tgctacctta agaccagcgg ctaatgcccc agctttcacc tctttgagcg cactccccgg
    53581 cccagcaaca gccgataatg tcaacaccca accacggtcc gcctcatcat aagtaataac
    53641 cctctggtta gtaaataacc ggttaggcat aataattttt tcaccactag ccgtttttag
    53701 gcgagtggaa cgccaggaaa tatcttctac cttagctaca gtgccatcat ctagacgtaa
    53761 ataagcgccc acctgaatag ttttatctag taagacaaaa agagcagcaa ataaattgga
    53821 taatgtctcc tgtaaaccta aggcaatggc tactcccgcg actcccaacc cagcccagag
    53881 tggcccaata tctatccgcc atatcttgcc aatgattaat aaaccaataa ggtatataac
    53941 tatctggcct gaccgtcgaa taattaatct catggtagga ttaaacttgg cagtccgatg
    54001 tctcttgtct aattgctcac tataatttgt taccacctga ctcaacaacc aacaaataag
    54061 aagaacaaag aaagaaatta aaatgttcaa taatgtagtc ataaaaatta tcaagtaatt
    54121 agctgttaag ttgttggcgc agggcctcaa gttcagcctg ccagtttgtt aatttagttt
    54181 gctccttagc aattatatgc tctggcgctt tgcttaaaaa ttccggatca ttaagacgac
    54241 ggcttaaatt gttaatggcg gtagttaatt cttggatctt gtgctctaac tgagcatggt
    54301 tgacagcact catggcttca ccctctaaat aaaaactaaa aggaccaacc gcgccagcta
    54361 aactgccctc tggcacctga ccgttcgcaa taattgtaac agtctcaaga ccagtgcgta
    54421 aattttgaat tagcttaatt tctttactta gggcatcagc ccattgtccc gcaataatag
    54481 tcatcttcat tttttggcta ggttgtaaat gatttaaact tctaatattg cggatttctc
    54541 caataagatc ttgcaccagc ttaaaatcta gttcttcctg ttctgcctcg ggattaatct
    54601 taagtacctg gggccaggaa gcaataagca aattatcttc tactcccaat tcgtcccata
    54661 aaacttgggt tacaaaaggc ataaaaggct gccacaaaat taatagatgc tttaaaatat
    54721 aagctaaaat attacatttc tccttactat cttctatttt actaatctct atataccaat
    54781 cagctaaatc gtgccaagta aaatcacgca aggcctcagc agctagagag aattcataat
    54841 gctccaaata attactaacg gcagaaataa ggtcattaaa ttgacgtaaa atccacctgt
    54901 cagctaaagt tgtctgagat aagtctagat tgttgtggac aaaaattgcc ttatcaggaa
    54961 taatagataa aatataacgg ccaatattcc ataatttatt aaccaaatta cggcaacttt
    55021 caattttagt agtccccagc cgacaatcat tgcccggggt aaccccaata agcaatgaca
    55081 agcgcactgc atcggccccg taaagttcaa tcatagctac tggatctaaa ccattgcctt
    55141 tagatttgct catcttttgg ccgtgctcat ctaaaactaa accatggaga taaacttcat
    55201 aaaaaggaat ttcacccaca gcaaaaagcg acatcataat catccgggag acccataagg
    55261 ttaggatatc ataaccagtc tctagcacct cggttggatg aaaacgagct aaatcaccta
    55321 atttgtgtcc tgcctgccaa ttatctggcc agcctaatgt tgagaaagtc cacattcccg
    55381 aagaaaacca ggtatctaga gtatccggat cttgtatcca cccagggcta gttggtgcag
    55441 tttcactcac caaaatttcg tctccctgat accacactgg aatttgatgg ccaaaccaaa
    55501 tctgtcgaga aatacaccaa tcatgcaggt tatccatcca ttctagataa cgtttagtaa
    55561 aacgttcagg gataaatttc atctgccctg tcttagctac ttcataagcc gcagtcttta
    55621 aggaacggcc acctaaccga gtaatgggct tgtcgacact gacaaaccac tgcttagatg
    55681 gcaaaggctc aatggtagtc ccacaccggt aacaaacaga taaattatta gtaatggttt
    55741 cttctttaat taataagcct cgcttcttaa gttcagccac aattagatca cgggctgcta
    55801 gggtggaaag accgctaaat tgtcccaaat tatctcccaa aagaccatca gtatcaataa
    55861 ccttaaccat tggaagatta tatttctcag ctaattgcca atcagttaaa gaatgagctg
    55921 gcgttacccc tacggccccg gtgccaaaat tacggtctac ggagcggtcg gcaatgattt
    55981 taatcgtcag aggctggcca caaaaatcca gagaataagt tttcccaata tactcctgat
    56041 aacgttcatc ttgtggatga acagctacgg cggtatcgcc taatttagtt tctggccgag
    56101 tagtagaaat agggatagga aaatcaggtg aatatttaaa agtataaagt gtagccatct
    56161 gttcccggta atccacttca tcatcagcca gcgtgctttg acagcgagga caccaattca
    56221 ccactctctc tccccgataa attactcctt ccttatacat ttccacaaac atatgccgta
    56281 ccgccttctg ccgaggctca tccatagtaa aagccagccg tgaccaatct agagaagcgc
    56341 ccatcttttt aatttgatct agaatagtag tctgaatcgg taaaacaacc tcccagacac
    56401 gacggagaaa ctcagtccga ccatagtcat aacgagtctt accttcttgt tgatagagca
    56461 tcttctctac cacattctgt gtagcaatag cagcgtggtc agtccctggc agccacaatg
    56521 tccgatagcc cttcgcccga tgataacgaa ttagggcatc ctcaatggct aacatggccg
    56581 catgacctaa atgcagctta tccgtaatat tagggggcgg caaaacaata gtgtagtttg
    56641 gcgcttccgg tggtaaaggt aaattgtctg gattaaagaa accactggct agccaacgct
    56701 ggtaaattgc tgcctcatat tttttcgcgt cgtaagttgg tgctaattca gtcataaaga
    56761 taagaaaact gctgtcagca taccaaaaaa gccctgaaaa agcaaggcaa aaagaatggt
    56821 gtcatttcta ccattagttt aatagttata aagaacctaa gcaccttagc taaatttcca
    56881 attttttaac aaaataagcg ttaatatata atacactagg caaataatta tccgccagtt
    56941 gtcaggccaa ttcactataa aaccagcaca aggcaaatga gctaaatact taataattgt
    57001 aagtaagata gtaagtagcc agccggcagg cataaaaata aaaacctgag cctctggcca
    57061 aagccagccc aagggacttg cgagtaagag aaaaatggtt agccatggta aagcccagac
    57121 cactaggaga ttagctagcg gagcaattac cgaaatctga ccgaattgcc aaaaaataag
    57181 cggccaagtc caagtctgag cggctaaagt taccgccaaa ttatcccgaa taagcgggta
    57241 gcgaatcaat ggcaaccgtt caatgagttt agtccaccac ttcgtccaag tcattaagcc
    57301 taaaactgca agataggaca gcccaaaacc taaatcgccc cacaaataga aaggattagc
    57361 tagaagcatt aaactagcgc taatgagcaa caagtggtag gcctgaccta accggcccaa
    57421 ttgccggccc cacagcatta aaaggcccag cagtccagcc cgcaccgctg aagctggcgc
    57481 tccaatcaga gcaagatata aaacaataac taggctggta gtagtcgaag cgagtaatcg
    57541 cggacaatgt aaaaggagca aattattaaa aagcaagata atgaggaggc taatattaaa
    57601 gcctgaaacc gcggtaatat gacttaatcc agtaagagct aaagcgttac gccactctgc
    57661 tggcaaacga cccgctccac cccaaataag agggcccgct agactggccg ctggctcagg
    57721 taaactagta gtaagaacgt cagctaaacg ttgctgacca ttaactaatt tgagccataa
    57781 attaagtggt tggcgaccca ctaattggcg cttctcactg gccataataa tccccactcc
    57841 tcggctaaat aaagaatagc ggtaactaaa caccccgccc tctggccaat tctttaattt
    57901 acccgtaaat tgccattcct cacccactcc actatctaac cagctatctg ggccataaat
    57961 catggcccga ccactgacac taacagtctc aacaccacac tgagctgact ttatcttaac
    58021 aattagctgt gaaccgccac tactacgggg ctgtggctga ccaactatct ctccccggat
    58081 agttacaatt ttattagaac aaataagttg atgagtggct gcttccttgt taactaacgc
    58141 ccaatgagcc cggccaaaac ccaaagctaa acataaaata ataagccacc gtgtcttacg
    58201 aggtataaaa ctactgcccc aagctaatcc tacgagccca acataataat atattgccgg
    58261 ccactgctca ggccataaac cagcaatggc taccccaata ataaaagcta gccaggggta
    58321 gctagaccga ctagattgtc cccctcctaa tgacattatg acaatttatt aactaaaata
    58381 attacaaatg tgttctgtat tatattctat ttattccttt ctaactccca taaaagatag
    58441 gcccgaataa aatcatcaag gtgaccatcg agaacagcgt ctatatcagt agttgtgagg
    58501 ttagtgcgat ggtctttaat gagtcgctta ccatatagaa aataagacct aatctgtagc
    58561 ccccagtcag ctttaacttg tccacccttt aagacctgcc gttttttttc tagctcttca
    58621 gcttccttat tggctaactt agaacggagg acagccaaag ctaaggcttt attttgatgc
    58681 tgggaccgct cactttgaca agttaccacc aatcctgtcg gtaaatgagt aatccggact
    58741 gcggaatcgg tggtattaac tccttggcca cctggaccgg aagaatgaaa aacatcaatt
    58801 cttaagtcat tagtattaat cgctggcgct tctgcctcag gcagttcagg ataaacttcc
    58861 accatagcaa aagaggtctg acgcaaatgc tgggcgttaa aaggtgagtt gcgtaataag
    58921 cggtgagtgc cattttctcc tttaagatac ccataagccc actgaccaac aaaccgcaag
    58981 gtagcacttt taataccagc ttcattcccc cgctgttctt ctataatttg actagtccag
    59041 ttctgacgca gactgtaccg taaatacatt ctttctagca ttgccgccca atcttgcgcc
    59101 tctactccgc ctgtgccagc atgaatagat aaaatagctg gtagtcggtc atggggctga
    59161 gtaaaaaacc gactaatttc tagttgatca atctctctgg caatctggtg atattctgcc
    59221 tgaactagtt ggttaatgtc ttcacttata ctattttctt gactaatgag atcagttaaa
    59281 tcatctaacc gttgtaataa ttggttataa ctctttaact cttgttcagt cgcctcaatt
    59341 tgctggagca actcaccagc cttggtgggc tgagaccaaa aatctgaagc catcgtggcc
    59401 tgccgccatt tagttaactc attggccttg ctagttaggt caaagacatc tcccaatctc
    59461 taggagacgc tcgcgtaagc tatccacggc ctctttatta attatttcta gtgacatata
    59521 ctagtaaact atttagaaat taaaggagaa agctggctaa ctaaattcct aataagcggc
    59581 gggagataga ccaccccagc tacaatagta agaataatta tggctatttt tatatacccc
    59641 aaaagacgct gtcctctaat atatttatag actctatgca cctccttttt tatttgagct
    59701 atatcggctg tctgctcagc cagcaaacgg cgccaatctt catccataaa taaaaaataa
    59761 caacaattat attataccaa aaaattaagt aaactcgcaa tcatctaacg gccacccctg
    59821 ctacctttgt ctgggctaca gctaaaaaat tattcatttc tacctcagta aaaggtgact
    59881 gtccagccag cgtactatct aacaaattgc catctaaccg gccacgttgt aaataccaac
    59941 gttgtgcccc ctttagctcc tccgctagct cggcaatatc ttgaagtccg aggaggccgg
    60001 gaactaatgt agttctaaat tcatagctga taggagtctt gtctatcagc caagtcacca
    60061 attcttttac cttagctact aaaacggtta atactgcttc aggcatggcc ataatctggc
    60121 tatatttaga ccaaggagcc ttcacatcta ctgccaaata atctaacaag ccctgagcta
    60181 ttaaatcttt aactatctga ggattagtac cattagtgtc taacttaacc aaaaatccct
    60241 gctctttaat tttctggata aatttcagta aatctttttg cagtgttggc tcacctccag
    60301 taataacaat agcctccact aaaccttgtc tctcggctaa aaaagaccag aatttagata
    60361 ggtcataacc accgccaccc cttaaattaa taagggaagg attataacaa taacgacagc
    60421 gaaaattaca accctgcgta aagactatcg ccgctatttt atctggataa tctaaaaggc
    60481 ttaaaggctc aaatccgcca ataatcatct taggactcct tagtctctaa ttggtcagca
    60541 cgtactacaa aagtacggcg gttagcaaat tcggcttgtt tagcatcatt ccactgatta
    60601 acaggacgta aatagccagt aactcggcta tatacttcgc agggcgtacg ttgagcttca
    60661 agggaggaag tcatatatta ttattaaatt attaaacaat tatttattag tcgctaccgt
    60721 ctcagcaata tagccaattt cctcgtcaca cttagggcaa taataatgct caccagggat
    60781 ataaccatgt ttaggacaaa tagaaaaagt tggggtaata gtaaaataag gtaaatgata
    60841 attttcagct actttccgca ccaaggttcg cacagcagct gcactaggta aagattcacc
    60901 tagaaagaaa tgaagtactg tcccgccagt atatttagtt tgcaaatcgt cctgcaattt
    60961 taaggcttca aagacatcag tggtaaaatt cactggtaac tgactagaat tagtataaaa
    61021 aggtggctca tcaggatgat cgctattagc agctttaata ttgggataaa gatcgcggtc
    61081 cagccgagct aaacgacgcg ccgtaccttc ggctggggta gcttctaaat tatataaatg
    61141 gttagtctcg acttgaaatt gaccaattat ctgacgcata aaatctaata cctcaccagc
    61201 aaactgatga cccgctggac tagcaattgt atcactaata ggggcaaaat taaggagagc
    61261 ttcgttcatc cctataaggc caatggtact aaaatgattg ctccaataac cgccaaagcg
    61321 ctctttaata ctcataagat agtgccgggc ataaggataa agaccactat cggttaattt
    61381 ttcaataagt tcccgcttaa tctctagact ctctttggcg agacgcataa gttttgttag
    61441 tcgatcaaag aattcttcct tggtatgact aagataccct atccgcggta aattaatcgt
    61501 tactacccca atagaaccag taagtggatt ggctccaaat aatccaccac cccgcttgcg
    61561 gagttcacgg ttatctaaac gcagacggca acacatcgaa cgggcatcct ctggatccat
    61621 gtctgaattg ataaaattgg aaaaataagg aataccatat ttacgggtca tctcaaaaat
    61681 tttcgccgcc accggcgaat cccagtcaaa atctttagtc aaattatatg tggggatagg
    61741 aaaagaaaaa acccgcccct tagcatcacc ctccatcatc agctcggcaa aagcagtatt
    61801 gagcatatcc atctcctctt gaaaatcacg gtaatgctct ggctgtaatt caccaccaat
    61861 aatcacattc tcttcgccta aggtcttagg gggccgtaaa tccatagtaa tattagtaaa
    61921 aggcgtctgg aaacccactc tagttgggac attaacatta tataaaaact cctgcattgc
    61981 ctgcctaacc tcatcataac ttaaatggtc agcgcgaata aatggcgcta gataggtatc
    62041 aaagctagaa aaagcctggg ctccagctac ttctccttgg acagtataga aaaaattaat
    62101 gacctgcccc agggcagtgc gaaaatgttt agctggccta gcttctactt tgccactaac
    62161 cccgccaaaa ccccgcatta ataagtcttt aatatcccag ccagcgcaat aggcggctaa
    62221 aatttgtaag tcgtgcagat gaaaatcgcc actttgatga gcttccctaa tgggagcgtc
    62281 ataaacctta ttaagccaat aagtggaaga cacaatagaa gacagatgat tattaagccc
    62341 ctgcaaacta aaactcatat tagaattttc tttcacccgc caatcacttt ggtctaaata
    62401 atcatccata atcttaccgg agtcaatcat taaagataaa tcacgcaacc gggcatgttg
    62461 gtcacgatag agaatatagc ctttggcaac cttaattaga cggttattaa ttaaggcctc
    62521 ttccactatg tcttgaattt cttccaccgc gggaatagta cgagcgtgaa acttctgatt
    62581 aagcctacta atcacttcat cagtgatagc ctgagccttg gtctcatccg gttctccagc
    62641 ggcaatcatc gctttattaa tagcagtaaa aattttattc cggtcaaagt caacaatatc
    62701 accagaacgt ttacgaatct tagtaatagt ctctcccata aagttacttg gttaatttaa
    62761 gtagtttatt aagctcctcg ctaaaatctt gcaactcaaa atccgaataa acactagcaa
    62821 agcggatata ggcgacctta tctacttttt ttaactgacg catcactatc cgcccaatat
    62881 cacgactgtc aatctcacct tgccgtctta cttgaatgtc ccgttcaatt aaattaacca
    62941 ggcgtttaaa ttggtcatcg tttatgggtc gtttttcggt agctttccgt agaccgctta
    63001 ttaatttatc ccggttatac aactccttac ggccatcacg cttaacaact gttaaatcta
    63061 aaatctccat ttcttcatag gtggaaaagc gaaaaccaca tttcagacat tcccggcgtc
    63121 ggcgaataga taaaccatca ctgaccactc gcgagtcaat tactttagtt tcagaataat
    63181 cacaaatagg acatttcata ttttgactaa aactacatat tgtgttttaa tttaggacct
    63241 aaacacaaaa tatagttaat tgtgattata ttataccact ttgagaaagg taaacaagta
    63301 gagaaaaagt ggctaaaata acaaaaacgt cagcgctacc actgacgttt taatttttta
    63361 aaaatattat ctaaaaaagt ttacattatc ttcttccgca aaaaagctgg aatgcttaaa
    63421 tcatcatctt cttcgccttc agatggttca ggagtttcag aagcagctgg cttagctggt
    63481 tcagcttgag cttttttaag ggcagacata aaacctttat gaccctcttt gacctcagtc
    63541 tcaccttctg gctgattttt tcctacaaaa ctagtcggtt tataataaga atcaaaagcc
    63601 ctgctaatag cttcgccttc agttgagacg gggttatcaa aaccggcagc aatcaccgtc
    63661 accctcactt catctttaaa attattatct actgtggtac caaagattat tctagcatct
    63721 tcatctaccg cattagtaat aattttagct gcttcatgaa tttcatctaa agctaaagtt
    63781 ggcccaccag tgaccgtaaa aagaacaccc ttggcgccat caatggacat ttctaagagt
    63841 ggtgagttaa tagccgcttt agccgcctcg atagcccgat tctcgccact agcctggcca
    63901 atacccatta aagtactgcc agcattaacc ataatggatt tgacatcagc aaagtctaca
    63961 ttaataagag ccggtgtgtt aataatttcc gcaatacctt ggaccccttg acgcaaaaca
    64021 tcatctacaa tactaaaagc ttctaaaata gtagtttttt tatcaataac ctgaaatata
    64081 cggtcattag gaatagtaat aatggcatca accttaccaa taagttcgtt ctgacctcgt
    64141 tcggcaatct tccgacgctg ggctccctca aaagtaaatg gtttagtcac tactgcaatg
    64201 gttaaagctc ccaggtcacg agccagttca gcaataatag gtgaggcgcc agtgccagta
    64261 cccccaccga gaccgcaagt tataaacacc atatctgcac ccttaagggc atctcttatc
    64321 tcattttgtg attctgcagc ggcatcacgt cctaaatcag gattcattcc agcccccaat
    64381 cccttagtaa tagttttacc aatgtgtatt ttgagaggcg acttattata atttaaatct
    64441 tgaacatcag tattaatggc gataaattct acccccttaa taccaccctc gatcatgcgg
    64501 ttaatggcat tgcccccgga gccaccaaca ccaacaacct taatgttggc tggcgcctca
    64561 acggctggct taacttcagt cataaaatat aaattaaggc tataaataaa atctttaact
    64621 tgattgtaaa ttatctacct aaaaaaatca agcaacctag tcttttaaaa ataaagacct
    64681 aaaccaatct ttaacccttt taacagcatg cccaggttta aaaccatggc cagaattatc
    64741 ttcttcctca acagaggaat aatgatcacc ccaagctacc agacctaaag cagttagata
    64801 acttaggtca ttaactttat ccactactga aggtacatta cggttagcgc ctaaacacac
    64861 cggcaaacgt aaattctttt tagctaaatc aacaatccca gctaatttag ctccaccacc
    64921 taccaaaact actccggccg gtagcatccc tgaccgacca attttcttta gctcagcatc
    64981 taccttggta aaaatctctc ccacccgtgc ttcaataata ctagatagat acttcattga
    65041 cacagtcatg ggctcatcaa cttcattatc gggagtaatt aaggaggtaa catctaattc
    65101 ttcatcctta tctattttat ctggattagc ttgaccatac tctaacttaa ttttttctgc
    65161 taaattaata gaacacctta aaccaatagc aatatcggca gtaatatgat ctgagccgat
    65221 aggcagaacc gctgcatgca gtaactcacc ttcttcaaaa accgctaaac tagtagtaag
    65281 agagccaata tttactagta ctacccccaa ttcttttgat cgaggggtaa cagccgcctc
    65341 agccgccgca aaaggggcaa taataatatt atcaatacgt aaaccagcct gcttaatagc
    65401 tttgcttaaa ttacgcacct gattacttaa tgactgaacg attaatactt caacctctaa
    65461 acggatgcct gacattccaa ctggattttt aacatcttct tgattatcta cccgataatt
    65521 aataggaata acatgcagca cttcataatt agccggtaaa gacagggcct gagctgcttc
    65581 aataactcgg ttcatatcct caaccgtaat ttcattattg gcctgattga tagccaccac
    65641 ccccttgcta cgctcacatt tcaaactagg cgcgcttaca ctagcataaa cactatctaa
    65701 gtgtaccccg attaatcgtt ctgatttttc caaacaggca agaaccgaag aggtaacctc
    65761 ctcaatatta ttaacaattc ctttgctcat accagctgtc ggtgccgcca ccgccccaat
    65821 tatctgaagc tgccccgctg ttacttggcc tactactagt ctagtttcag ttgaaccaat
    65881 atctagtccg gcaattaaac tttgatctct catagtatta tattatagtc gtaaataact
    65941 taattactcg tggtccaaat aaaaatacac caaccactac agctgtggcg gccacgagaa
    66001 gaactgcacc agccataata tctttaattt ccttaacata aatatctagc cgtggtttta
    66061 ataaatcagc agcccgctcc actgcactat taaatacttc catcagaagc actaagccaa
    66121 tggtcaaaat aaggattgcc cattctacgg ccttaaacct acttaacacc gctaataata
    66181 caacgcctac ggctatagct aattccaggg caaaattctg ctcctctcca ataactcgcc
    66241 ataggccacg acaagcatag cgaaaacttc tccataaacg attgccgctt agcatatatt
    66301 atgtctattt aaaaattgtt caccaatttt aatcatcgtt tgccgtccct cttcactgtc
    66361 atcttcatag cctaaaagat gtagcaaccc atgggtggta ataaaaatca actcctgcca
    66421 caccgaatga ccaaaccgct gagcctgtct tacaatctgc ggataatcaa taataacctc
    66481 tcccaattga ccattttctc catcagcaaa ggctaaaaca tcagtaattc tatcttgtcg
    66541 gcgatatttt ttatttaaat tacgaatccg tcgatcccca actagtacta aactaacctc
    66601 gcctttcacc ccatactcat ctagaaataa ctgacttaat ttttgccatt tgaccatatt
    66661 tactttctgt ctggttaaat tacaaaccga caccataatt atttaataat taaattatca
    66721 atcattagat gaaatatatt attataactc aataaataat tactttccgg attgtaagtc
    66781 aaggcaatta aatcatcaag ccccggctta gtaaggtaat aagtaagacc gtctgggctc
    66841 ataacaactt cattagcacc atgagtagta atctctggta cctggtcggc gtcaacatta
    66901 aacatttctt gataccagct agctaaatcc tggctagcta ccttaccaag tatagttaat
    66961 tgaataaact ggccatcatc agcacgaaaa atagcggagt tatcacgacc caattgctga
    67021 gtccatgaag ctgggactat aactgaccat ggcccatgtt caacaagttt aagattacga
    67081 ttattggtta atttcccctg accagcagga tcataaccag aacgtatctc agttaaatca
    67141 tcataaccat caccatcact atctgctgta tcagatttag tgcctagcag ggcctcctct
    67201 ttatcggtta agccatcttg atcactatcc ttatctaaat ttaaatcttc tatcaataat
    67261 tctttggtag tagtagcagt tgatgactca ctaacttccg gagcaacaac ctctgtagtg
    67321 ctggtggtta cttcgtcttg ccaatgttca ttatctaacc gccactgccc atcttcaaac
    67381 aacatagtaa ctatagccac tcggccagta tcagccttgg taatagttaa agtagcttga
    67441 ttaccattaa tttctttagt aattttgtca ctagcttcta aaggaatcag catagactta
    67501 ctaatactta aagtgctaga tttttgactt tctgataaat tatttatttc tgctgcctgt
    67561 tccgttaatt cttgggcttt agctcggcta gcaccggcaa taaaagcctt taaataatct
    67621 tctaaggtta aagcctggtc taactgtaga cgcaactgca aataggcctg ctctggatca
    67681 gataaatcaa taatgacctc gctactagta gtaggactac tttcagttaa agttgtggtt
    67741 ggcatgctgc tggttactgc tactagttca ttggttggtg caggattaaa attaaaaata
    67801 gggtttttag taattattaa aaaaccaaca taaactaaac cgaccaacaa taataaacca
    67861 accaccataa ttaataagcc agccttatta gtaaagctgg ttctatggcc aaaacgaaaa
    67921 gcactgggca tagtataaat atactggccc tcttcactac ctggggtagt ttcattctga
    67981 ttaacaaatt caatctgatc agcgggggga ttaagatcat cagaataagt gattttaaaa
    68041 ttattagtcg gcatttccga catagctcaa tgaatcatta acttgactat tattggctca
    68101 aacgaccctc acctaaagga ttatagccat tcttcacctc ctcaccatca agataaccat
    68161 caccatcagt gtcagatttt agcggattag tcccataaac attaagctca tcagcatcag
    68221 ataggccgtc ttgatcacta tctaaattaa cgacctcgtc tgaggggagg gattcttcag
    68281 tttccacggt ttctgaaatt tctggttctt cggggaccac ctcttcactg ttattagaac
    68341 catcagtctc tagcgaggct tcatcagtag aaacagcctc ttcggtcagt tcattaataa
    68401 taactggctc actagtggct atttccatta attcttcgcc attctcagga ttactagctg
    68461 gcgttgaggt atctacgtcg gcctcttcaa cattaacaat actagagggg atatcaactg
    68521 ttactgttgc ctggcgagat ttttgaacat aaatcaaagc accggcagca ataatgacta
    68581 aaaccactaa aataataaca atcggcaaca ccttactgcg aggtttagcc gatatttggc
    68641 cagtaccttg attattgctg gccatctctg gcacattatt aatagttcca aaggcttgtg
    68701 agtctccagg agctttggga ctagccttat ccacattagc aaaaatgtcc tgcaccgtcg
    68761 tctctccctg gggagcgcca gtttctggct gatttttaat agcctcagtc tctttattgt
    68821 ctttattctc cgaagccgca ccgggagtcg aagatttttg accattaact tcctcaaaca
    68881 taagttttat tattataaat aaactaaatt attataacat aaagtcatta aagagccaaa
    68941 taattacttg ttgcattcgg atccataagg tagttgggct gggctaacat taccagccgc
    69001 atcaactgca taaacattaa caataactcg cttggggtta attaaattag taatagaagt
    69061 tcttacttta ttcccactag ctggctgact taccaataaa atttgactac tttcactggg
    69121 tgaggcggca atacgatatt ccactctata ccttactgta tcatcttgat tacgattcca
    69181 cactaaattt atttgatgct caccatcagt tcgctcaaaa caatgtaccg cactaggtgg
    69241 cacaatatct tgaggccgag cttgcacttc agctgaaaat ggagtctcag caaaggcagc
    69301 tgcatcaaga tgtatcaaag ctgaaacagc aaaataatat agttgtccat taattaaatt
    69361 atctattgtc acctgggtac tagtagttat tatggggtca acaaaatact gtccactatt
    69421 taaaccataa tacactttat agccgctggc attagggtaa gcatcccagc gcaaacccac
    69481 tttccctcca gccgggaggg gtgtagctac taaattagtt ggtgaacttg ttactgaaga
    69541 atagtggaaa aaatagaaag ccggaaccat tgcccctgga tcagtgctag ttactacctt
    69601 gagagctgga aggtctcctg cttggctgct tccttcacga tcccggcaat aataaaattg
    69661 catattagta acactatccg tccatggctg ccagctacca tctggccgac gagctggcca
    69721 agggttggca cataaaaata ctctaatcaa tgcagtctta gaaaaactag gcaataatgc
    69781 tccaggtaca gccgtctggt ccgccgtaac ctgtgcaatg gcctccacat aggtttcggc
    69841 gtctttttta ttttgtggtc gtaatagcgc ccgattaccg ttataactca ccaactgagc
    69901 cacggctgga ttacctatag accataacca ttgccaatta tatgaaggat aaggcactaa
    69961 tatctggccg ttcaaatcta cagctgtcgc ctcaatagta ctactagcct gcaaatctga
    70021 aaataatatt gaggtaggat taagttctac atggtcaaca cgacatacct gatcactagt
    70081 cttaaaagac caatataggg cagctggtaa ctctagattt ccaaactgta acggttttcc
    70141 attggctaag cctataccat tttggtctaa aatgccttca ggttgagaat cttgaagatt
    70201 gttatcagct ctcataacca cataaatacg gcgattagct ggtaaaacat tggctggatg
    70261 aatagttaaa tgcttaccac ttaactcaat tgacttgggc agagcgcaat aagtattatc
    70321 tggcaaaatt ccctcctcag ccgctacatc gggcctgaac caagtcagta atctattttt
    70381 taatttaacc caccagccac cctgccccaa atcagctgca gctaaatagg tagtaccagt
    70441 tggacaaggg tctttctggc cataatcagc aatcaagaaa atattatctt ctaaatgggt
    70501 agcggccatc gtcttagaaa attccacgtc aataaggcta ttacggcaaa cttgttgcgc
    70561 ctcattgtct ggcaccgtct tcactatata tggcctagct cgacaacaag aatcctgacc
    70621 acatccatca tatattatac tcccacaatc ctcatcccgc tcacaaccac agcataagcc
    70681 ccggttagcc cccgaacatt gatcacgatt agcatcacaa tataaattgg gacttacttg
    70741 actgcattga tcatgggctg gatcacagca acaaatacca caatcagcct gattagtgct
    70801 agttgagcta agcccactcg ccgtacgaca attaaagggc gatgcacaaa gttggtaacc
    70861 acaacctacg tggttaatat aattaggcca caaaaatgta gtagtggcca cattacaaga
    70921 ctcaccttgt aaattgacaa cctggcaact agcctgacat tcagctaggg tagtaaattg
    70981 gccttcagac tgtaattcag caatacactt gttattatta caaacatagt agccacaata
    71041 gccattaacc aatctattac cttctaccgt accacggcat tgactagttg cctgatcata
    71101 gcaacaagta gtgtgagcta ggcaatcctc tttgcccaat tgggcgcagt cgctacaaat
    71161 accattggct actgaggtat tacaatactt acccgttgtg cccaaacagt tacaagccat
    71221 atctctagta cttgtagttg gaccaacggc agcacaaccc gaacaggcac caaatctttc
    71281 ctccgaacca caagtcccat tacaagttaa gggcaagcaa caaccaggat tagtctggtc
    71341 aggtctattt aaattttcgt cacaacagca ctcacataaa cctgcctctt tccggcactg
    71401 attattgcta cattgaaaac cactagcaca aatcggctca tcaaggccac agcgcccttg
    71461 agctcccacc gccaaacact gaagaggcgt ttcacagtta aggcaggcca tattgctacg
    71521 gcaaatatta ttagctaata attgccattg tccatattcg ctagttgtgg gcggacattg
    71581 accagcagtt aaatgagtag tccaatgttt tccttgacaa gttaaagcat aattaactgt
    71641 attgtctcct aatttaaaat taaccttctt atctaaatca cataagacat tgctaatgca
    71701 tctattagtg gttgaattat aagtaatctg taaaggagcc acgccggttg aatgcccacc
    71761 accactagtt tgctcaccaa atagttgggc acagtaacta tcaccacaag ggccatcaat
    71821 agtagaacca ggtttctgac tacaccagcc aggtgaatta ggacaataaa gtgctccatt
    71881 acactgatga taatcgttgc acgataagat ggtcttattg gtacagacat tattaacaca
    71941 ttgttgatca gcagagcaac atttactccc acaaattgtg gtattaggtg gacaggtaac
    72001 attgccactg ccagtagtaa aagaccaccg ataaacacag ctactagctg acccataaca
    72061 agcagctaaa ttatcacgac attgaccagc atcagctgat ggagcgggac aacaaatctg
    72121 tccagggcaa ttatcattag tcttacaagc tcccaccata aaattaagac cattacttac
    72181 tccctgaata ttcttaacac ggactatacc actaatggct tgagctggta cggtagaaga
    72241 agctccgctt actgtaccat catcaaacca actaactgaa gtagcaataa tatttggact
    72301 aaattgaaat cgactggtca tcggatcata attcctaaaa tgttctcccc atacttcaac
    72361 ttgagtccca acttgaccag ttgttggacg aatagcacat aaactaggag ccaaggggtc
    72421 agaattaaca ataaataatt tgctagcctc taatatatcc cccgtaccta attgaataga
    72481 caaaagctgc ttaccctcca aattagcacc acttggcact tttacaataa tttgtcgatc
    72541 cgaccacact gccgtagcac aaatttcagg aaaattataa tcagcctcag tgctgccaaa
    72601 gaaaacatga ctattacctc taatattgcc aaagccatga ccatagatgg taacatattg
    72661 accgggcggc ccgctcatag gctcaaaacc agtaatctgt ggcccactcc caggatcaac
    72721 taacttatta aataacaaaa aattactcgc tggccctggt aaaataggat tatcattgcg
    72781 gacaaaagta ctaacccatc caggctgtaa actaggtacc caagctttta ataaattgct
    72841 agccattact tctgattcat tagcaggaat aggattagat aaattaccat ataaagccac
    72901 cgtagaagtt gctccagttt ggataaaatt aagtccccga taattaataa tctccccagt
    72961 ggttcctgag gctttatcca aagaacacaa tcccggccgg ctaatattat taataattaa
    73021 ctcctgacta ctggtagcaa taaaattgtc actatttttc acctggacta tcccggttgt
    73081 tgccccgctt ggcaccttaa caataatttg attattcgac caggccgtgt tgccacatac
    73141 attcaaatca cctaatttat cgttagcaaa aatcaccgaa ccagtagacg agccaaagcc
    73201 agtgccaaaa atagttatta aattaccaac tttagcatta ggagagttcc tatcacaagt
    73261 atcaccattg gcacattcgc tatccgtatt acaatattga ttataattat tacgacaaaa
    73321 accgccctgc ggacttaaat cagtaatccg tggtctattt ctacacaaac aacaacttgc
    73381 cgtacccttg ggataaccag ccgcctcgca ggaggccgcg ctagatcctt gtgtagtaca
    73441 aaaagcagaa taacataaat tagaattaca gcttgttcca ggagattgtt gatcgttagc
    73501 acaagctgcc ccctcacact taagacaact gccaccacag tcaagacctg attcatcggc
    73561 gtcctgttgg ccgttgcaac aatgagccgg caataatcgc cctctctgtt ctatagtcgt
    73621 tcgtcgacca gcaatatcgt caactaaagc ctctagagtg aaaggctcac cggctgtgct
    73681 tgtaggctgc caatcagcaa tagccgtata atttaaccca aaatcattag tagataaata
    73741 aaattgctgg ccattagcca aaaattcaaa tctggctaga ccagtatcat cactagccat
    73801 cactttaact agattattag tcgccgcaca tagaccaata gtagataaaa attcagcctg
    73861 tggatcttga caatcgacaa gagaaccaac actaaaacta agatcacaag aaaatcctaa
    73921 cccactacag actaaattct taggattgtt ccctaaactc ttaataccag aagtaagatg
    73981 gacagtaata tgactatctt ccggcaaaca gttaacctca ccacagctac tactacatat
    74041 actagctggt tggaaggcta tctctcggcc attaacagtc cattgaccag taagtggttg
    74101 atcatcagct tgaactgctg cactagcatt ggttacagag tctggatcaa tggagccatt
    74161 aaattgaaaa cgaattaccg tattacgtgg cacacccgag gcaccatcag ctggactaac
    74221 tccagtaata attaaatccc cgcttggttc tggtggtcca ggtgggcccg gaggcgaagg
    74281 aggtgcagaa ccaccacccc ccgtggcctc taataagcta ttaataataa aggttgtaat
    74341 agcaaaagcc gataaaacaa taacaagtcc gatgaccgcg ccttttaaaa tatttttagc
    74401 tttagtaatc ttatcctcat taccgttact agtcatccag acaaaaccgg cataaataat
    74461 taaaataaca gcaatagtcc ccaaaaaccc caaaactatt ctaataattt tagctacagt
    74521 tatacgtgga tcctgagtag aaagaccagt agaagaagca tattgaagac ccaaatcttc
    74581 actcgtaagc ccctgagctt gagccacaga taaatgtccg aagctagcca aactaactac
    74641 tattaaaccg cttaaaaata tcttcttacc ccaagacata agattattaa ttacataaag
    74701 gtaaagcact ggggagaccc tcacagcaag aacctatagc gtcgccgcaa gtagaatcat
    74761 cacgacaggg tttaaaacaa ttctgccgaa tgtctttcac ccccgagcca atctctatga
    74821 aatatttagt agcatcagat agtagcccgt gattaataat aactttggta aagtcaggat
    74881 aaccgtccgg aatgccagca tccaccgcct cgctagaaat ccaataggat ggagctaggc
    74941 cggcgctgaa taaattaatt aagtgatgat gcatatcgtt tataatcgtc tcgccactaa
    75001 gcaaggtgct cgccatcata agtttatcaa aactgaactc aatgggctga tttagcaatc
    75061 cttgagttga atgcagggct ggtatcaatg aatcagcttg caatctgggt ggggtcaaat
    75121 caatctcatc gctcacaaaa aaagaccagc gataactatc cccgtgggca ctatcttgat
    75181 cattctcatt ataaaaatta actgggccta caactttact attacggttg ccgtctagtg
    75241 agttaaagca gacatcaact atgccaccgg ttaaaggtaa gttggctaaa ggataatggt
    75301 ctccttcggc ccgacgacaa gaaccattaa aacattgatt atatggggtt ttacttatac
    75361 aatctttatc ctcagtacaa ctagctagag cagcagcctt tagcttaacc acaatagtac
    75421 tgctagctgg cagacaataa attttatcac cacaagcatt gacaccacat tcagtatcag
    75481 gaataaactc cactgtttga tattggttag aaatgacaaa acgaccgcta ataggctgac
    75541 tgctagtggt aagtacttca atagtggaac ttacataatt actatcccct actactgtaa
    75601 gaggattaat tggttcagaa aaattaattt gaataatgga attacgaggt gcatcaggac
    75661 gatcaacaat ttgaatattc tcttcccggt cttgcccacc actaaaaggt gttagggata
    75721 aatgagaact atcggtagag agactcaaaa aattaccact ggtcccagcc ctacgagctt
    75781 ggagatacac aatattgcca tcactttgag cactaacatc aatattagtt ccatttattt
    75841 tattagctat attgtcagct gtaccctgaa tcgtagcggc aactacaata tgatcagcat
    75901 tactgtcggc tgaactaacg aaacgatagg tttgactacc aactgttaag gtttctcctg
    75961 cttgagctgc cgtaacagca attttccaac tttcccctgg cgatagtcgg ccatgattat
    76021 ctgggggcaa agaaccattt ttaattaaag tcagcccaca gcctaaacta atatgaccat
    76081 cactcacatc atcatcatta ataaggccag aaatgccaat ggcctgagca cttaaagggc
    76141 taccaggaac cacagtcacc gtaatagtcc cactatcgct acaattatat gtaccctcta
    76201 cccgggctgc accggcatta ccctgagatt cagctggacc agttaccgcc gaagccatcg
    76261 ctaagggtaa agacacaact tcaatactag cagtagccac ccgggcaagc tgggtattac
    76321 catgaatatc tcgaccatta tcaggaaaag gcaaaatgcc tcctagtctt acttggggtg
    76381 gagttaagtc aacccgagag ctcacctcaa actgccaagt aaaatcactt agagtaaagg
    76441 ccggctcatt attagctttt agtaatcctg tagttaattt aactgactgc cagacattac
    76501 cactagctga acctaaataa ttaactggaa caaaaacaaa agttttgcca tcattagtgc
    76561 ttaccttcac ttcatcacct gccaaattag tagctggatt atcaccaacc tctgtcacaa
    76621 agaatcttat attattggcc ttaatattag tgttgacaca aattccggtc gcatcattac
    76681 cgccgtcaca aattgtagaa accttcatct tctcacgaaa agttacaata acactagtat
    76741 tgcgcggtac atcagtttga cccggagccg gataaacact ttctacaatt ccattgccca
    76801 gggctccaaa agtagaagta gtaaccccac caccacctcc tcctccacct ccccctccac
    76861 cacctcctcc agcaccaatt aacttatcaa taataaaagt aacgatagaa aaagataaca
    76921 aaataatcag taagccaatg cctgctgatt ttaatatgtc ttgagctttc ttaatttttt
    76981 cagcattacc attactggtc atccagacaa aaccagcata aatcactaaa acaacggcaa
    77041 ttgttcctaa aaaacctaga gcgatctgaa caatgcgggc aataactatt cgcggatcct
    77101 cccgagataa cccggtttcc gccatatatt ccaatcctaa attatcccca ttttcttggg
    77161 ccatggctgg aaaagaagga ccaacggcca gggtaagcat aaagaggata accccaagcc
    77221 cagcaaaacg acgcaataca gtggtcatgt attttttgac tgattagaaa aattaaagta
    77281 tagttctagt atagcacaag ctaacttaaa ctaacaaggg gggtcttatg actattgagc
    77341 aagttatcgc ccaaactcaa ggccatctat ctaaagcaga tacagagtta atcaaaaggg
    77401 cctataattt tgccgccaag gctcataggg gacaaaaaag gataaccgga gaaccatata
    77461 tagagcatcc tcttcaaact gcttttattt tggcgcaaat gggtttagat gctaaaacta
    77521 tcgctgctgg cttactccat gatgtgccag aagatagtaa atatacatta tcagacatca
    77581 aaaaaaactt tggtgaagat attgctttct tggttgatag tgtcaccaaa ctcaatctta
    77641 ttgaatacca cggccgaact agataccggg aaagcttaaa aaagatgttt ctagccatgg
    77701 ccaaggatgt gcgagttatc tttattaaat ttgccgaccg tctgcataat ctaagaacat
    77761 tagaatattt gccagaagac aaacgacaaa aaattgctaa tgaaactatg gatatttatg
    77821 tccctattgc tggactcctg ggtatttggc accttaaatg gcaaatggag gatttatgtt
    77881 ttaaatattt acaccccact gaataccaag aattagtcca aaaatatgaa gtggaaaaaa
    77941 aattggaatt aaatcaatat attgaacaga tggccacaac tttaagccag gtccttaccc
    78001 aattaaatat tgagcataaa attactgggc gttttaaaca tttatatagt atctgggaaa
    78061 aaatgcatga caaaaatcgt aaatttgatg aaatttatga tgtttttgcg ttacgagtta
    78121 tcgttcctga tgtagataac tgctaccgta ccctgggcgc tattcatagt ctctggcgtc
    78181 ccaatataaa ccgctttaag gattatatcg ccctacccaa acctaatgag tatcaaagct
    78241 tgcacactac ggtctttggc cctggtggca aggctactga atttcagatt agaactccag
    78301 agatgcatga acaagcacaa tatggtatag ccgcccattg gagttataaa gaatatggca
    78361 gtaaacgacc aacccccaag gtaggctgga ttcaggagat attaaatgct caaaaaaata
    78421 gtccggatac ccaaaccttt atccagcaat tgcgcttaga tatctttagt gaccgtctct
    78481 ttgtgcttag ccctaaaggt gatgtttatg agctaccagt tggggctaca ccgattgatt
    78541 ttgcttatgc tgtccatact gatgttggca acaaagctaa gggcgtatta attaataata
    78601 aaattagtcg ccttgatgag ccattacaca ataatgattt agtagagata atcattgaca
    78661 aacagcaaac aggtcctgat ccggcctggc ttaaatttgt caaaacaact aaagccaaaa
    78721 atgccattag acaatatctt aaaccctcgg ccttaaatcg ctttgccgcc ttggctaatc
    78781 ctaaacactg gtggcctcga catcataatc ctaatcaacc accagaaaaa aataaaaacc
    78841 tagagtagga cgctatcttt aaattaatga aagtcgtctt tgatttggcc aatttttctt
    78901 agatattctc tcttttcccg cagagcttgt cgcattaaag cactctgttt agcctgacgt
    78961 ttatttggtt ttttgtaata ataatgcttc tctcgcgcta acattagctt accatcaagc
    79021 tgaaggcgtt tattaaagcg cctaataaaa ctttcaaagc tttctccggg tttacgttta
    79081 aattctacca taatatttta cctttctagt tattatttag ttttagctaa acgtttcaac
    79141 ataatagact taactctttt taaatctaaa acctcctgcg accctgattc catatcacgt
    79201 aataaaatag taccatctaa taattctttc tggccaataa ttaaagcgta ctttactttt
    79261 agttgattgg ccaacgctaa ctgactcttt aaactatttt tagtaaaagc ttgatgaact
    79321 gtcaagccag cttgccgtaa ctcttcaaaa agctgtaaag attttaagcg tggcccatca
    79381 cctagttggg ctaaaaagac atctggccga gtttgactga cctctacctt gttggggtca
    79441 agatataata ataatctctc catgcctaaa gcaaaaccac aagctggagt aggccgacca
    79501 cctaacgact caattaattg gtcataacga ccaccgcccc ccagtgctaa cggtctttga
    79561 gctgactcac tgggcataac cacctcaaag acagtgcgat tataataatc aagacctcgc
    79621 accaattgag aattcaaatt atattggatg cctaaattat ctaagaactc taaaaccttt
    79681 actagatgtt gcttacaggg ctcacaaaga aaatcaatta gctggggggc accttgagcc
    79741 agggcctcac aggcaggttc tttacaatcc agaagccgta aaggattttt aattagcctt
    79801 tttcgacagt cagggcataa ctgttctcga tgtttctttt ctttataata tttaactaat
    79861 tcattaatat aatttggccg gcagtcaaca caacccaagc tattaatatc tatctgggct
    79921 tcaatgcccc aatcatttaa aagctcatga attaagaaaa ttaattctac ctcggccaga
    79981 ggaccaggct caccaaaaat gtcaaaatta agctggtaat gctgacggta acgtcctaac
    80041 tgcggtttat ctcgacgaaa caatggccca taccataaag tcttaactgg ctgaggaagg
    80101 ttaaaccacc catgttcaat ataggctcga acaatgccgg gtgtggcttc tggccgtaaa
    80161 gctaaacgct caccgctttt gtctaaaaag ctatacattt ctttagttac aatatcactt
    80221 gttcggccac ttgaccgttc atatagggaa taactttcca atacaggagt atcaatccgt
    80281 tcaaagccac gtagtctagc caaccgacca gcagactgta aaagaaattc ccaggccgga
    80341 taagctcctg gctggatatc cttcatgccc ttaacgcggt gagctatggg cccagttggt
    80401 cgggagggcg tgacgaggtc aactaactta tcatgctttt tagtcataca aaatagtcaa
    80461 ttatattaat aagttggagc cttaaaaaac tgaagacgct tcagtggaaa gcctctaaat
    80521 attacccggc caattaaata gcgacgatta actggcccaa aataacgaga atcccgcgaa
    80581 tgttctcgat tatctcccaa gacaaaaaac tcatccggtc cgacagtaat ctgcttagtc
    80641 tcaaaagctg aagtctcatt actactgctt aaatatgatt ctgaaagttt agtccaagaa
    80701 ttattagact tatctaacac ataaacccct ccgttttcta ttttcaaagt ttctccaggc
    80761 aaaccaataa tccgcttaat aaaatattcc ctaggatctt caggataacg aaaaaccact
    80821 acatcacccc gttgcggctc tctaaaccga tagcttatct catccaccag tagatattcc
    80881 ttatcataaa aattgggctc catggaagcc ccttgaacat aaaaagggca aacaataaat
    80941 aatctaatag gaatgacaat ggcagcggaa ataatgacaa tcttaacaat ctctaaaaaa
    81001 gacagccatc gggctcgcca gcggacctta aaatcaggag taaaaatcat gaagataaac
    81061 taataataaa ctaagagctc ctaaatgaag cttgagctta atatagcact ggaaggacag
    81121 ctggtcaagt tgccaaaaat aaaaaaatct tttatactac taaatggttt tgcgccttac
    81181 aaaaccattt tttgtcataa tgctgtcata cttatgaatc aaccatttaa tgccccctct
    81241 gatgaccttc tagatgaaat gctcaatagc cccgaaaata atcctgaggc caattctgag
    81301 ccactggaac cggcagataa tctctccgaa cccattaata ttactattga agctaaacaa
    81361 agccatcaac cccgccaaga accaagagaa aataccccct cactacacca ccctgccatt
    81421 attgaaaaac cacggccacg taaaaaacat catttaggtt attttttctt aattattggc
    81481 ttaattcttt taggatattt aggctggcgt gtctggtcag cgactcctgt aaatctagaa
    81541 acaaataata gtagtggtaa taaacaagaa ttatcttttt ggaaaagcct ttctctgctt
    81601 actaaattta aactggggat tcacgagcga gtcccgctta aaggtgaagc agaagataga
    81661 attaatttta tccttttagg aatgggcgga ccgaatcatg atggagccta tttaacagac
    81721 actatcatcg ttggcagttt taaaccatca actaaacgtc tagttttaat gagtatcccc
    81781 cgtgacctca gtgttcccat cgctggttat ggctggcgca aaattaacag cgccaatgct
    81841 ctggctgagg ctaaagagcc aggcagtggt ggccaagcta ctgctgattt aattggcgag
    81901 attctagaaa taccaattca ttattatttc cgagtagatt ttgccgggtt tgtcaaaata
    81961 attgatgacc tgggcggcat tgaggtagac gtagacaata ctttagatga ttatagctac
    82021 cccattatgg gggaagaaga caaccctgat tattacaaac gctttgaaca tttacatatt
    82081 gatgctggcc accaaacgat ggatggagaa ttggccctaa aatatgctcg ctcacgccac
    82141 gctgtgggag tggagggttc agattttgcc cgctctaaac ggcaacaaaa gatcttaatg
    82201 gctattagag aaaaactttt tgctgccagt aatttattta aacccaaatt aatcatgaat
    82261 atcgtcagtg atgctgaaaa tcatatatct actaatttaa ctccagaaga actagtaagc
    82321 gcctggcaat tagctaaaga tgttaaacca gaaaatatta ctactattaa tctagataat
    82381 agcccgacag gcttactgta tgatcaaaaa ggagctgatg gcgcctatct cttggtgcct
    82441 agaacgggtg attttcggga aattattgct cgatttaatg aggccctgct aggttcaacc
    82501 actacagcaa ctattaataa tgaggctgta agttcaacta gcgatatctc cagtcaacca
    82561 accgccgaat cattaactaa acaaaataac actattaatc cagctgattt accaactaaa
    82621 gccaatgcta aattagatat ccgcaatagt actaatataa atggtctggc cgcacggact
    82681 aagactaagt tacaaaaata tggctttacc gtagttaatg tcagcaatac tagtcataaa
    82741 aatctaccaa ccaccactat ttatgacctc acctatggcg ctaaaccaga tgatttagct
    82801 ttattacaat accaaactaa agcagcccta gcggatgata tccctaactg gttggcccaa
    82861 gagactagtg aacttattaa agaacaacca gacatgactc agccagattt tattcttatt
    82921 cttggccagg attactctgg taaaaaccaa taatatgcca actagtaaac cacagccagt
    82981 tattatcttc ggccgtacta atgttggcaa atccactctc tttaatcgct taggcgacac
    83041 ccatcaggcc ctagtctcct caaccagcca tactacccgt gatgccaacc ataaaattat
    83101 taattggaat catcaatatt tagagctaat agatactgct ggccttagtg agcttagtta
    83161 tcttactatg agtcctaaaa aagcagcccg gcttaccgag ctccaggcta aaatgcagta
    83221 ccaaaccgga gaacttctcc ggacagccgc cgtcattctc tttgtggttg acgcccgggt
    83281 tggtctatta ccaggagata aagaattagc ccgagcttgt caaaaaatag tctcacctaa
    83341 tactccaatc atcttagttg ccaataaagt agataattct cgttatcttt ctgaaatgac
    83401 ggtcttttat accttggggt ggagtgatcc ttggccggtc agtgccctga ccggtatggg
    83461 gactggcgat ttattagatg aagttgtacg gcgagttaaa aaacaaggct cacgctcccc
    83521 ccaaaagcca cccttagatt ataactccac tgctaaacca gattatcggg tctgtatcat
    83581 cggcaagact aatgtgggta aatctagcct gtttaatagc ctagtaggga ccgaacgaat
    83641 gctggtcagt actcagccgc ataccacgcg cgaaccacaa agcgccatct taaaatatca
    83701 agaaaaaact attgagatta tagacaccgc cggactaagt cgccgtcagc caaaagctaa
    83761 aaataatcta gaaaaaagga gcatgattaa aagcctggca tgccttaaag atgcagatat
    83821 tgccctctta actctagaca ttagcgaacc aattacccat gaagaagcca gactggctca
    83881 agaaattatt gaccataaaa aaagtttaat aattgtagct aataaatggg acaaaattac
    83941 aaaccatgac cctaaagaat ttagtgctct agtttatgac cgattaccgt ttattaaatt
    84001 tgcgcccctt gtttttgtct ccgctaaaac tggagctaaa gtccagaaaa ttctagactt
    84061 aatccttgag gtgggccaag accgcgcgat taccctctct cccagccagc tcaatcatcg
    84121 gctcgcccag atagtgaaac atcagccccc aacccaaggc cgaggattaa aacatcctca
    84181 tatttatact cttagccaag taaaaactaa cccccctatt tttactgtaa ctctagggcc
    84241 taaagataat ctccagcctg cctatctgcg ttatatccaa aatgaaattc gcaaatctta
    84301 tggcttttta ggtaccccca ttactatccg aattaatcac cgccgttcct ctaaaccccc
    84361 agaactttct taataatatg gaaaaaatta ttatcgggct tggcaatcct ggtcaaaaat
    84421 accatcatac ccgccataat gctggctggc tagtactaga ccggctagtt caagagttgc
    84481 tagatgaacc tccccactgg gaaaataaac cccggctctt cgcccaaatt attgttatcg
    84541 gccacaacct cctagtaaaa ccaacaacaa tgatgaatga ctccggccgt tcagtcaggg
    84601 ccatagtaga ttattataat ttaaccccag ctgattttag tactaaacta ctagtaattc
    84661 atgatgatct agatttacct ttagggcaaa ctagatggtc taaaaatagt cgttcagccg
    84721 gtcataaggg tgtccaatcc attattgacg ctctgggaac acaaacattt aatcgctggc
    84781 gcttaggaat taaaccagct tctcccttgc tggaagaacc tgccactttt gtattaaata
    84841 atttctctcc cctagaactt caaatacttc aggctttaag cctaaaacca atatataatt
    84901 ttttagaaga ccagccttct aataattaat tttcattttc tcccggactt cttccattgt
    84961 ctcccgggtc cgttgccgag ctttggcagt gccgtcccgc acgatatccc gaactttagg
    85021 catatcctga gcataatgag cccgccgctc ttgcagtggc gctaaataac taattatttg
    85081 ttgcgctaaa caagccttac actctacaca gcccaacttg ccagtttgac aatcatgggc
    85141 aatggtcttg gcctgagagg cattaaaaat ttgctggtag ctatatactg gacagatgag
    85201 aggatggccg acatcatgac gacgaacctt ttgcggatcg gttaccgctg ttttgacctt
    85261 tttctcaatt actgctggct catcagcaag ataaatagca ttatctaaac ttttccccat
    85321 tttaacactg ccatctaagc ctttaagacg tggaaaatca ctaaagatag cccggggttc
    85381 aggaaaagtc ttgccataaa tagcattaaa tttatgagca atctcccgag cctgttctaa
    85441 catcggttct tggtctgcac caactggtac tgcttcagcc ttaaaagcta aaatatctgc
    85501 cgcctgacta atgggatagc ccaaaaaccc ataagtaact ccacccttaa aaagcgactt
    85561 cttttgttcc agctcactct tcaccgtggg attacgttct aggcgactta gggtgactaa
    85621 attgccaaaa aagacagtca gctcagcaat ctctggcacg agagactgaa taaaaattat
    85681 tgctctatga gggtcaaggc cagcagctaa attatctaac actacctcat aaacattatc
    85741 tttaattttt tctggctgtt taaaatgatc tgtgagtgct tggacatcgg caacttcaac
    85801 ataagtatcg tactctgctt gcaactttac ccgggtaagg agagaaccaa ataaatggcc
    85861 aatatgtaga cgaccggttg gccggtcacc ggtaagtatt gtactcataa ttaaagtaaa
    85921 aagctaaaga aatagggcta agttggctgt aactctttct tttctttgta atttagtaaa
    85981 tcccgtaagg ctacggtgaa agctgtcgta atggggatag caatagccgc accaattact
    86041 ccagccagcc tgtagccaat catcaagaca ataatactgg caatgggatt aacatcggct
    86101 acccgcttca taatctgggg tactaaaaca ttattttcta attgctgaat aataacataa
    86161 acaataataa caactatggc cagacgaggc gattgagtaa gggcaataag aacagcagga
    86221 ataccgccaa ttaccggacc taaataaggc acaacctcag taatcccagc caccaaagct
    86281 aaaagcaaag gatatcttac tcctagaata gaaaggccaa ttagggtaag aagaaaaatg
    86341 gctaaacaaa gcagtagttg tcccctaaac caatagccaa tctgcagttg catccgagtg
    86401 accaaattaa cagcataagc ctgactgcga gctggggtaa ccgcccaaac cattttttta
    86461 atggcatttt cttcaaccac catgtaaaaa cttaatacta aaattaaaaa gagagaaaat
    86521 aacccattaa ccgccccata agcagcagta ataagctgca tggtccagcc agaaatagta
    86581 ctagccaaac tatctaaatt agtctgaaaa ctagctgcca ggccatattg ccccatagtt
    86641 tcccgcaccc ggcccaaaaa ggatgagagc ttaccaaaat aatatggtaa actgttagct
    86701 aagtcagtcg cttctttaat aacgacaggt actagaaaat aaaacatcag gactaccaag
    86761 cctaatacta ctaaataaat taagctgatg cttaatgccc gaggccattt gtgccgtcca
    86821 agataatcta ctagcgggtc aaaggcacta gctaaaacta aagccacaaa aatcacagaa
    86881 aggacttcct taataagaaa agccacatac aatagaaaca aaatacctac caccttgagg
    86941 atagtagaag tagtaatatt aatgtgaatt gggttattaa ctttagctgg cataaaaatt
    87001 aaaaaccact aaaataataa caaaacaaat tatctattta cacgtaacgg gcatattctg
    87061 agcataataa taaggaacat acgaaacaca gttcctatta tctgcataac cagacgacca
    87121 ggctatatat ttcgacgttc cacaggcact agtgggaacg ttgtatacat agctgcagcc
    87181 atcaaaagaa ccccagggct cacagcattc atttggcatt gcttgagcca acgaaatcaa
    87241 agatagtaat tgcttaccat cccgatatga tattcctcct ccgcctcctc cgtcatcagt
    87301 gcaacaaccc ctacctttct catactcaat atcacaatac tggtgacgat agatgtagcc
    87361 gacagggata gggtcaacgc tttgacagaa cttagattga gttgatctaa cttcaacatt
    87421 accattgcag tcccaatcaa agccaaatag tgggctggaa gttataacct gattagtaaa
    87481 tgtactagaa tgataaacgg cggcacctgg cactgcagct gagttattat cacagcaatc
    87541 atccaaacca attattatat ttctgctatt ataaccagag ggagtagaag ttccaactga
    87601 gtaaatctgt tggcctaagg cccacggtag gctatgccgg tcattatcat tatctggtac
    87661 atataaataa gcctgcacca actttgcccc agtgttaata gttatcttcg c
//
LOCUS       DAOOIS010000002        70736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_433_length_70736_cov_40.801616, whole genome shotgun sequence.
ACCESSION   DAOOIS010000002 DAOOIS010000000
VERSION     DAOOIS010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 70736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70736
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_433_length_70736_cov_40.801616"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..92
                     /locus_tag="PK960_00495"
     CDS             9..92
                     /locus_tag="PK960_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HPD07689.1"
                     /translation="MELSKKYGLYRQRYCGCKFSIDKNNIK"
     gene            249..1292
                     /locus_tag="PK960_00500"
     CDS             249..1292
                     /locus_tag="PK960_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPD07690.1"
                     /translation="MDLQFYFEQAIARKASDIHLVGDSAPYLRIDGQLVKLSEEIIDA
                     KWLAEAVYGFLDQRLVERLENNKELDVSKTIGGSHFRINLHYQRGVIGLTARLISSVI
                     PDPEFIGLNENLQHLAFLNDGFVLVVGPCGAGKSTTLASLLGIINRERQGKIITIEDP
                     IEYIFQNDHCLIEQREIGIDTNDFAVALKYVLRQDPNVIMVGEMRDNETIEAALTAAE
                     TGHLVFSTLHTATAAESLSRVVSSFPAHQQSQIATKLSLCLRAVIAQRLLPAASGGRV
                     AAREIMFNTPAIANLLRSQKLEQIPSIIQTSSREGMITFNRAIEELLLSGKITSEIAK
                     IYERADSGASYWR"
     gene            1304..2062
                     /locus_tag="PK960_00505"
     CDS             1304..2062
                     /locus_tag="PK960_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07691.1"
                     /translation="MSSTVKRTPILIGLLSLASLEIINLRVYFIYWLPLIMVIVMFVV
                     SWFGLAHHRGTYKIIAWLIPALNFLAWCGFLTIINSSFVRQLFIIIVAVSQWWYWYEG
                     LKEIPASQLRLGEWWRTVNVLNCCTVWVIASVLYGWQSFLGWPIWWPWLMWFLCLAII
                     IIGDYYVSDLKLSVHWPMVVATWLILGQLFVIIYFLPSSNFVLAYLLLLAYYIFGQLN
                     RNNLLQINTKKRLRYQIIALMLGLILVISTAKWF"
     gene            2075..3166
                     /locus_tag="PK960_00510"
     CDS             2075..3166
                     /locus_tag="PK960_00510"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HPD07692.1"
                     /translation="MKFKTTHKKLSPNFWWWLLAVVVNLVLVLIWFKYFFTLGEADLL
                     ASMALSGQGMGVPMSSNRITDLKHTVAIDYNEAASLWPSLSASAIQIFKSQDKIQITI
                     NGKTENGYNLDKPLASFWVVTNDGWLVTSQDFSDWSSLIAVTNDHRTHTISKKLIDPL
                     TGLVFYRLEGANNLVPLKLGENGWSRPGQLFYNLNGRGGFQTSWLEKVNYNEADSLTI
                     NSDSLRQRWQFSTIWPDSALFNGAGELFGLVNGENEFIPVNYIKSGLTSLLSGDAFKR
                     VSLGLEYIDLNQLSLNNHYGLLITQVIKDSPAAKAGVKAGDILLSIDEINLDGEVSIN
                     EIIQNYRPHDRASLVIQRAGQNHQLNCQF"
     gene            3170..4351
                     /locus_tag="PK960_00515"
     CDS             3170..4351
                     /locus_tag="PK960_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPD07693.1"
                     /translation="MPINTIASLPQGEGRKIFLRLDLNVPLEGGHIKEDYKIRAARYS
                     IQELLKRDFRLILATHLGEPMVNGQLIPGALETLSVKPIVTYLSELLSETVEFVPGQD
                     FKKIRQQIDKSPSRLFMLENLRFWPGELTNDPHFAKDLASLADFYVNDAFAVSHRQQA
                     SVAAIKDFLPSFAGYLLEQEVVNLSQALSPHQPLIVVVGGAKINTKVNFLRHLGEKAA
                     KILLGGALANNFLAARGYNVGQSLVDKESIALSGEIIKELGDKLILPLDVIVKNQGRP
                     VVRSVNQVADDDNILDIGPATMQAFSREIKSAKTLVWNGPMGKFEEDHYQHGTLFVAR
                     EIAWRSRGPAFGIVGGGETVEALEMTKMTNYVDWVSTGGGAMLAFLGGEPMPGLEKII
                     Y"
     gene            4367..5635
                     /gene="eno"
                     /locus_tag="PK960_00520"
     CDS             4367..5635
                     /gene="eno"
                     /locus_tag="PK960_00520"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014514424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPD07694.1"
                     /translation="MAKIVQVAAREILDSRGDPTVEAKIILDNGLMAKASVPSGASTG
                     VHEAHELRDGDLERYGGKGVLRAIANISEVIGPKIIGEDITELSKIDHLMLELDGTAN
                     KEKLGANAILAISMACARAGALATGQELYSYLASSYGFSTSNYRLPIPSFNIFNGGKH
                     ADTNVDFQEFMIIPLVDTLVAEKIRLGSEVFQALGRLLKDNGLDTNLGNEGGYAPNMP
                     SSETVIDFIIKAIQIAGYIPQGQVGLGLDVGSSELYDEASGRYIFKLDQWQLTAAELI
                     NKYKEWALKYPLVSIEDGLAEDDWDNWQILTQELGSQLLLIGDDLFVTQAERLREGVE
                     KKVANAILIKLNQVGTVSETIDCIKLAQQSGYKIMISHRSGETTDDFIADLAVAVGAE
                     YIKSGSLARGERLAKYNRLMEIEAQLAGKF"
     gene            5642..6562
                     /locus_tag="PK960_00525"
     CDS             5642..6562
                     /locus_tag="PK960_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07695.1"
                     /translation="MSQVVADNKMKKISPVVLVLLDGWGIAPPSIGNMFSRAHWPHIY
                     NYLQQYPIAALASPLASNGTRKVAYWQIGSGQEVEMIPGLGGVVDRPGLTELLVQAGL
                     RQAYIGESETFPLVVEYLANHCLHNLVDYLLVSTPVNNDYNKNVLSASHLVIERAAHC
                     WWHYQLDFMVVSLASWALVSRYGDESSVVKAIVAMDRLLPKLINPLLQAGVTIILTSA
                     YGILENYLNPLDNKINHGLSGNPTPLVIIGRPWRGQSLGETEVITTDLTTLAVIGSLR
                     SIAPTILTILGIEKPDYMTDPLLPATAINR"
     gene            6566..8194
                     /gene="murJ"
                     /locus_tag="PK960_00530"
     CDS             6566..8194
                     /gene="murJ"
                     /locus_tag="PK960_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPD07696.1"
                     /translation="MKLNKLWYGSINNIGWAAVIVGLSSFISRFLGVFRDHILAGQFG
                     AGDELDIYYAAFRVPDLIFNLLVLGALSAGFIPIFVSLLDKPEKEEDNKAAWRFANIV
                     TNCLLIILIGLCIIGIILAPQIIKAITPGFSGEKLSKTIELSRLMFLSPLLLGLSGIV
                     GGILQSFKRFFAYSLSPIFYNLGIIAGALFLVPHIGLIGLAWGVVIGALLHLLVQVPT
                     LRHLGFRYQFILDWRDKHLRQIGRIMIPRTLGLAVSQLNLLAMTVLASKLASGSLAVF
                     NLANNLQSFPVGIFGLSFAVAAFPTMSALVADKDKLRASLTATIRQTLFLVIPSTVMI
                     LGLRAQIIRVILGSGRFDWQDTILTMRTLSYFGISIFAQSLNAILVRVFYAYKNSATP
                     FWISLVSSLVNISLAWLLSGQLGVAGLALAFSVGSIVNLILLWIFLRIKAPVLDGRAI
                     ADGAIKMVIAAAISGLAIMEAKKIIAPLVNMRTFWGIFGQLCGAAIIGAVVYLAICSL
                     LRCPEAKELWMMISHKWPSKKKKMVVDAEEARGV"
     gene            8267..8797
                     /locus_tag="PK960_00535"
     CDS             8267..8797
                     /locus_tag="PK960_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07697.1"
                     /translation="MLPYVSLLIWRRASQPHGIEILLALRSAFQFEDASRVQPQDFYR
                     GWQIFPSGKLGSDENSPLSAAWSKLRDDGGVRAMIGLKALVGSNPTRLRQLDQNCFAV
                     EIKESDDFDGESNFLNLVNLSPNYILVAWKSLAEILHFNDLALYRHGVPKGVEAIFRD
                     QLDLIKKLADQVRRTG"
     gene            8815..9246
                     /locus_tag="PK960_00540"
     CDS             8815..9246
                     /locus_tag="PK960_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07698.1"
                     /translation="MAFGTGKAINQPIKKQVEEMVALKGYDVFTQPDLAEILELKAGH
                     LFVANQYGNYISTLAFMQQFVLMAKVYGWKSVRLVAARQHIWRCARDLRHLAPEMHVQ
                     LYPVTVEYGPDTQLWVRSSIGWWAREIVIRLLPWPIYKKIC"
     gene            9763..11547
                     /gene="lepA"
                     /locus_tag="PK960_00545"
     CDS             9763..11547
                     /gene="lepA"
                     /locus_tag="PK960_00545"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPD07699.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLELTGTIEPRKMTNQILDRL
                     DLERERGITIKLTPVRLKYRGYELNLIDTPGHVDFSYEVSRSLAAVEGAILLVDATQG
                     IQAQTLANLYLAIEQNLAIIPVVNKIDLPAADIAKTKGEIVKLLGCAPEEIICVSAKT
                     GEGVPKILERIIEVVPAPIGDEDKPLRALIFDSNYDEYRGVVAHIRVVDGIIRRGERI
                     KLVATGAVSEALEVGFFDPNYHKSQELRAGEIGYVVTNFHEVSECRVGDTLTLEKEAQ
                     KIIPLKGYIEVKPMVFAGVFPKEGDAFENLREAMGRLKLNDAALVYEPEQSQALGYGF
                     RCGFLGLLHLEIFQERLRREFNLDLIVTVPSVAYKLYRRNGSEPEIIRSPQQLPNGAE
                     IDYLEEPWVRLDIITPKEYLGAVMKLAQEKRGIYRNTEYLDATRVILHYEMPLSMILT
                     DFYDKLKSLTSGYGSINYEMLDYRRAEVVRLDIMVADHLVEPLATIVYRDEAFKRGKE
                     IVTTLKETLPREMFVIKLQAVIGGQIIAAERISALRKDVTAKLYGGDVTRKRKLLEKQ
                     RQGKKKMMALGRGQVDIPSEVFVKLLKH"
     gene            11685..13400
                     /gene="recJ"
                     /locus_tag="PK960_00550"
     CDS             11685..13400
                     /gene="recJ"
                     /locus_tag="PK960_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPD07700.1"
                     /translation="MAPPRLWQIAPSLKKSNSELAELGLPFIIEQILYNRGLDKADEI
                     RQFLGLQPFLANLADLSQLSLAVEVIVKHIKAGNLIVVYGDYDADGVTASTILLETLA
                     ILKAKTRIWLPDRVTEGYGLNTGAIDEIAQWGAKLIITVDTGIRNKQEVAYAQKKGLE
                     VIVTDHHRESPDPAEWPDCLIINPTMTREHYPCKFLAGVGVAYKLVQAIVERSTLSDN
                     QKKTLERRCLDLVALGTIADCVPLLMENRQLVKEGVEIMNTRRRIGLEELARVAQISG
                     DINEWNISWQLAPRINVAGRLDHANSAYRLLTTKDQAEAAQIADDLNQKNNERQQKTQ
                     EIVDFCLSELLELQADDSLLIFVSPRQDPWPEGIIGLVAGRLAERTGKPVIVITKGEQ
                     GYKGSGRSNVENFSLIALLEEVASDLDRYGGHAGACGFNLKVRQLPNFLTNIKKLAAK
                     KLANLDLRPKLFIDGVLPIKDIDMNIAEWIEKLAPFGQANPEPKFVAHHEVIDDVSTA
                     GLSQQHLKLRFGNYWALAFGQAEDYSDLRPGQLVDLVYNLGINRYNNHAELQLKIIDL
                     RRINN"
     gene            13414..13857
                     /locus_tag="PK960_00555"
     CDS             13414..13857
                     /locus_tag="PK960_00555"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HPD07701.1"
                     /translation="MTKKFVHQSLISYTDGGARGNPGPAGIGVVLKTKNGETVGEWSK
                     FLGITTNNQAEYRALLLALEEARSLGVEELECRLDSELVVKQLRGEYKVKHPDLKPLF
                     AQVLSLISSFKHVSFNYIPRTLNKEADHLANQAMNQEMNRKYKDT"
     gene            13848..13921
                     /locus_tag="PK960_00560"
     tRNA            13848..13921
                     /locus_tag="PK960_00560"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13882..13884,aa:Val,seq:cac)
     gene            14029..14414
                     /gene="rnpB"
                     /locus_tag="PK960_00565"
     ncRNA           14029..14414
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK960_00565"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            14427..15839
                     /locus_tag="PK960_00570"
     CDS             14427..15839
                     /locus_tag="PK960_00570"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPD07702.1"
                     /translation="MKRSELFFSFILLPIDFIMIVLAGISAYYLRISDVATGVRPIIY
                     NLPYKDFLSLLLLIALAWLVVFALANLYQARPIKWFDELRRIFFACLAGFGLIVIYIF
                     FFREWFSSRFIILVGCALAWIYVSIARLLVRWLQRYLYRYGIGVHRVVIVGRGASADK
                     LIKEWFSHRSHGYQVVKHYDNFDLETAQELEDYLKIKPVDEIIQADPNLSKAEVIRLY
                     DFAEEHHLVYKYIADLLDTKILKTEVDEIAGIPVVEVKKTPLDGWGKIAKRIFDLVFS
                     TFFIILFSPLYLLTAIAIKLDSSGPVFFSRLDDGSPLYRIGQGGRKFRYFKFRSMVPG
                     TDSLRYTALADRNIRSDGPMVKIKDDPRITRVGKFIRRFSIDELPEFFLVFIGSMSLV
                     GPRPHLPEEVAKYERHHKKVLTVKPGVTGLAQVSGRSDLSFEEEVKLDVYYIENWSLA
                     KDISILLRTPMAVFRHRQAE"
     gene            15841..16947
                     /locus_tag="PK960_00575"
     CDS             15841..16947
                     /locus_tag="PK960_00575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD07703.1"
                     /translation="MKVALIHDHLAQDGGAERVLRVLAEMYPQADIYTLLYEKRQADK
                     YFSDRTIKTSFIQRVPGGVKHYQWFLPWMPLAVEFFDLSDYDLVISDASAFAKGVITG
                     INVPHICYCHTPTRYLWDYTHRYIAELKYNKFIKKVVSLCLNYLRVWDYQAARRVDYF
                     VANSSLVQKRIKKYYDCESEVIYPPVEVDNFKMGEGQGDYFLIGGRVVTYKRFDLAVE
                     AFRELGWPLKVFGDGIDLPRLKKMAKEAHNIEFLGRVSDKERAELYQHCRAFLNPQEE
                     DFGITVVEAMSCGRPVIAYSQGGALETVVEGETGVFFKEQTKESLIEALHRFEQLSFN
                     AVAIRQHAVKFSRQRFETQFRDFVERAINDFRSK"
     gene            16951..18093
                     /locus_tag="PK960_00580"
     CDS             16951..18093
                     /locus_tag="PK960_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPD07704.1"
                     /translation="MKVAVDARPLLAKETSGIPEYVGFLIDALTTAHPEVEWQLFYSS
                     WREKPWQWEWLGERPNVSWFPLKCPNKLINAAAWLIDQPKLDKYCPADLWLLPHFNFT
                     PLSGKTPAVLTIHDLSFLRQPEFFSWRRRFWHASLHLSRLVNLAKKIVTISESTKRDI
                     IELLGVPEDKITVIYSAVARQFQPLARGDQQLIDCHHKYNLPQRFILSLATCEPRKNL
                     VSVIEAYNLLRRRRTNYDDCQLIIVGGHGWRQGLARKVWQGSPYREDIRWLGYLPAED
                     LPALCNLASVVVYPSFYEGFGLPVLEAMSCGRPVITSAVTALPEVGDGAALLIDPADV
                     NALARSIEQVLANEELAKELSRQGLKWAQQFSWEKTAQEYWQILAG"
     gene            18177..21707
                     /locus_tag="PK960_00585"
     CDS             18177..21707
                     /locus_tag="PK960_00585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPD07705.1"
                     /translation="MPQPTFVHLHVHSHYSLLDGMGKIDELIDMAKEHNAPAIALTDH
                     GVMYGAIEFYQKCRKAGIKPIIGVEFYVAPTSRHDKGGQAERSYHLILLAKNEVGYHN
                     LLKLVSIGHLEGFYYKPRIDWECLQEYSGGLIALSACLSGEIPRLILAGKYTKAKERA
                     LAHNKLFGEGNYYLELQHHPNLPNQVEVNRELIRLSGETGIPIVATNDVHYLRTSDRE
                     VQDVLLCLQTKHKKQDSGRMNMLDDDYSFRSPEEMAQAFIDTPEAINNTVKIAEACNL
                     ELPMNQVLLPHFEVPQGFTAQSYLRQLCLEGLVKRYGKTYDEIEAVYRERLDYELDVI
                     DKMGFPDYFLIVADFINWAKSHGIVVGPGRGSAAGSLVCYLIGITELCPISYDLLFER
                     FLNPERISMPDIDTDFADTRRDEVINYVADKYGRDHVSQIITFGTMAARAAIRDVGRV
                     LGASYDFCDKLAKMVPMFMKLPEALEQVDELRQIYQESPQAKQILDYAQRLEGSARHA
                     STHACGVLITKEPLTEYSPIQYASSTDRTIVSQYSLHSIEDLGLLKMDFLGLKNLTII
                     ESALKIIKVTKGVEIDINRIPLDDERTFELFQNGETTGVFQFESSGMKRYLRDLKPTN
                     LEDIIAMVALYRPGPMEWIPDYIAGKHGRKKLQYLHPKLESILAKTYGVAIYQEQVME
                     IARSLAGFTMGQADVLRKAVGKKIPKLLAEQKEKFITGCMANGISKTLAQKIFSFIEP
                     FAGYGFNRSHAACYAMVAYETAYLKANYPTAFMAALLAADQQNSDRVAIEIEECERMG
                     IEVCPPDINQSFDSFTVVDENLVDHPTIRFGLLAIKNVGEHIAKVIRDERKRGGPYQD
                     MGDFLRRINDKDLNRKSLESLIKAGAFDSWLDRRQLLENIEVLLKYNKEASQNSLNGQ
                     YSLFAGVDGVKDPPLYLRPAPPADRQTKLSWERELLGMYITEHPFVDYHRELKSVIVP
                     IKNLAAKAPGEIVTIGGMVVSSKKIITRGSQSMLFVKIEDDTGNLEVIVFPKLLEVTR
                     EVWQAGQPILCRGKISDKDNERKVVVDKAIVLNLDHISDDLAAFRQSAANMKPGFWAV
                     KPVSYKVKSADLLSKSPEAVHLILRQSISQAQLFKLKEILLHYPGPQQVYLEIDFNGE
                     KRKIATGVQVKSGPELLAELKAEFAESIQF"
     gene            21739..23184
                     /locus_tag="PK960_00590"
     CDS             21739..23184
                     /locus_tag="PK960_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07706.1"
                     /translation="MSAQKTSIAPKSRGSGARRPAGRPPKVRPLGRPSKSSIARNVPN
                     KVKTKTHEVKVPKPKVVVVTNQPLVSEEEFSVWQAAREEEPKNEEFSVVPNENEDINI
                     SSKPLEPPELPDNHQPSTRLYRNLAIFFIILIGFVIMVMFYYSFIKVKIILYPSASTQ
                     QQNATIYLTDITPVSNGYLPAKFVEQSTEYRETFKATGERQLPTAGKLVGQVRLINKT
                     GKDQPLVATTRLLTSSGVLYRLKNFVTVPAGGDIKAEVYADEVKPENAIEANTRFTIP
                     GLWSGLQDKIYAIAEEPIVFQSNIEPIILDSDLDQAIAQAKEGAAKQAKSLISQGFQD
                     KAIAYWLDDTKWEVKSDGQVGEARSEFTVTVKATVGAAIFDLQAMIDLINNKLDNSLV
                     IDESSIAYQLVDWQPQEHYAELNVSYTASIVATRADQLIDKRALVGLTHEELEDYLSK
                     QPGLATYKIIWQPHFLKKVPGTDKIILQLAQ"
     gene            complement(23189..25198)
                     /gene="gyrB"
                     /locus_tag="PK960_00595"
     CDS             complement(23189..25198)
                     /gene="gyrB"
                     /locus_tag="PK960_00595"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017895889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPD07707.1"
                     /translation="MNQNFKKATTEYNAETITVLEGLEPVRKRPGMYIGSTSSIGLHH
                     LIWEVADNSFDEVMADYADMVEISLLPDNLVEISDNGRGIPVDIHKTTGVSALETVMT
                     KLHAGGKFGGNTYKISGGLHGVGVSVVNALSSYLKATVYRNGEIWEQEYQYGKPLNPV
                     KKIGQTDSNKHGTTITFRPDATIFDTIEFKWGTILDHCRQQAYLTKGVTVKLHDRRPG
                     HRHKDYNFYFEGGIKSYIKNLNRNNQVKNKTIFYAEKEINNVKIELALQYNDGYNEII
                     LAFANNIINTEGGTHIIGFRSALTRVLNNYARSKGLLKDKEENLTGDDVREGLVAIIS
                     VKLTNPQFEGQTKSKLGNAEVKGYVEQVCSETLGAFLEEHPKDAEQIINKCLLSAQAR
                     VAARTARTAILRKSALEGATLPGKLADCSSHRAEECELFIVEGDSAGGSAKQGRDRRF
                     QAILPLRGKILNVERARLDKMLENNEIKNIVIALGTNIDDQFDITKLRYHRIIIMTDA
                     DVDGAHIRTLLLTLFFRHFNPLVTGGYLYIAQPPLYQIKKGNSIFYAYSDEEKDKIIN
                     DLGSNNANIINDEAAESKIEPDEAIQNKGLAKVHIQRYKGLGEMNPEQLWQTTMKPDC
                     RILKQVTVEDAAMANEVFEMLMGDEVMPRKNFIQTHASKVINLDV"
     gene            25355..27496
                     /locus_tag="PK960_00600"
     CDS             25355..27496
                     /locus_tag="PK960_00600"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HPD07708.1"
                     /translation="MKKNLFNNKLLEESSGDKPKYHSSLSDKWLWLIAGLLCIIFVII
                     VFCLIMTWNQKPPSAIGSRGTEPESKQPEEIALPVVTESLTNNIAREGSNFQADQLIF
                     SQLYQQPTTSWPVEILANKAQSITLPINVKKDVANYYEIKRYYNLEPIISQLNKEGSV
                     LVDNPFGDEKQDFFDFYHSATENHWPLLLTSDVIFYYYQNNIKEVYKAVEAGFYDDLW
                     QISNGLFKIANQRYQAAISQSLKGNDPLIEAYRLNAQYWAVALKLLAPKDNQISNDGI
                     TSNSMIFRTGDDQIYKLPVIDEDLRREVDQEVSLIQAASGVKISPLFGYEMDYGLFKP
                     DENYQDGGRLANFLLVSKWLNYSWPLYYREAGCTKCPLDIDDWRVNFLAANVLARDLN
                     SDQEFKNYWAKIYKIISYFSGLRDDLTYVHYIAAQKEILGDQSLEAVARLDEASRNQV
                     LDRLRVAILGNKFLPTNGGWDRQQPDNWPKIGLRVLQDRYWPGQFIDWQTVGPKLDVF
                     VSQQTTTDWQVNQFWSSMEMIKEWLPKILSANSEWVRPAWWKAQHLSLAKFYLLNWQL
                     PADQRRILLPSANSLFQNDGVTENIVSIDTASLPAIDRQIATAKMLQLALVKLQASNL
                     SADQKLMDTINTLEMIRDLAVKQAKGEEWTKEESTAINNFFQSAIVRSGSKQLDPSQK
                     DSPYLQKIKARIDIKMINGQLYILIGPIIMP"
     gene            27519..28403
                     /locus_tag="PK960_00605"
     CDS             27519..28403
                     /locus_tag="PK960_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07709.1"
                     /translation="MPTKNQASNKVLPDQITDDDVSLKKLSFGLWYMRHRRLFLNIII
                     VVLIIFSASGWIYSFYVLGHYFIFDRQKDQQLLSEMAQTSVNLLAGRTNQPLSISDVK
                     VLGSGPNNLLAEIYNPNPRTAVFFNYAFVLNGQPIVQRDGFVLPGEKKYLVALNQNIP
                     VGASVYLKIDQARWQRLDARQIPDWPAFLAERNQLVIADRQINLADNSNRLISQVKFR
                     IENHSAYGYWSVPILIIAYRDGNLVDVNELVLSKLRSGEVRNIVFSWPGLKDVDYIEI
                     IPEVNFLDQENYLPIDTD"
     gene            28407..29507
                     /locus_tag="PK960_00610"
     CDS             28407..29507
                     /locus_tag="PK960_00610"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPD07710.1"
                     /translation="MHLRFSQYWRYNDWPVTLAILLLLAIGLSQIYGVALGRGGTSLV
                     AFYKQIVFVIVGLGGYILLSVVDYHRWRSYARYLYILAVILLITVLLFGSSIHGTKGW
                     FNILGLGIQPVELVKVILIICLASYFSGRTIRLNQSKQFLISGLLTFILTFLVLLQPD
                     LGSALLLVIIWLFLILAAGFNRKYLYWVIIAGIIAVVVGWLFLFTDIQKNRFRSFFNP
                     NYNVSGQSYNINQAMIAVGSGRLLGRGLGFGSQTQLKFLPEAQTDFIFAVIAEERGFL
                     GAGLVLLLFAVIIYRILTALPKVKDDFGCFVIVGAIGLLATEVFVNLAMNIGLLPIVG
                     LALPLVSYGGSSLISTLLLLGLVNSIIIRSKI"
     gene            29512..30273
                     /locus_tag="PK960_00615"
     CDS             29512..30273
                     /locus_tag="PK960_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPD07711.1"
                     /translation="MKIPDFNPETIIHGRLMKKLERQGLRNDSIYPIPEGMERVWQVF
                     IDGQKVDHYSHLAAKSQYCLMNIGVSLDKGQYVTLQEADSSGAVIIPFVIIQNEKLVY
                     LNKLNKKNNYLVYAGLIRQWRLRQGGWVWNVPRGYANLGENSKKTAARELMEETGLAG
                     KLFLLPGQPLNPNSTYFETGHNGGVIIYAINLGPDLADWQNNKVTLRHRPRPTGIDET
                     IKACRFFPLARLSQLGDMFSVAAESRLRYYLENLR"
     gene            30365..31030
                     /locus_tag="PK960_00620"
     CDS             30365..31030
                     /locus_tag="PK960_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPD07712.1"
                     /translation="MKSELRLEAKTRELGRQSLKKLRAAGFIPAIVYGPQTDNLPLQI
                     KKSEALKIYEQGGESSLITLNIDGQDERQVIIKNISQSSVTNHLLHIDFFQVNMAEKL
                     TTEVELNFVGEAPAVKQLGGVVVKNFDAVEIECLPKDLVSHLDVDISGLTELGSHITF
                     ADLKLPKGIALSSKHEMNEIIVAVEEPRAVAAEAEVTEEVSAEEVPSETAPEAEVSGE
                     EQK"
     gene            complement(31033..31932)
                     /locus_tag="PK960_00625"
     CDS             complement(31033..31932)
                     /locus_tag="PK960_00625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HPD07713.1"
                     /translation="MKKRWRKTSTINPPTIAEARQWLEQMWPLSMQQPALASWLLASI
                     TNRTSSELITCAQQPLTTKNWHSLQKITTKLQKNYPLPYILKEQYFYGRPFKITPAVL
                     IPRPESECLIDIATRLRQETNKPTVWLDIGTGSGCLLITLAAELNNKDYYGGSDISRR
                     ALRLATHNAISLKQKNIVWRHGSLLEPWLKDSFDSAQQIIIIANLPYLTPKEYNQQPS
                     LQYEPRRALLGGPEGLTLFQELANELPGFIKYHPYHTINVLTEHNLNHNQKLPSLLAV
                     ANLKWQQTFCDLDGQPRFGWWHN"
     gene            complement(31898..32959)
                     /gene="prfA"
                     /locus_tag="PK960_00630"
     CDS             complement(31898..32959)
                     /gene="prfA"
                     /locus_tag="PK960_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPD07714.1"
                     /translation="MTLQELSDQLNQLDAQLNNPATIANAKEYNRLVKERAAVADLIS
                     LATRLEKIESQLTYIDQLLSDNTDPDIIALATEEQQQLLIEKTTLSQQLEEALHPADP
                     RDKKNVIMEIRAGAGGDEAALFAADLFRMYSRFAENHNFQLEILNSSPIAIGGYKEII
                     FSITGEKVFSWLKYESGTHRVQRVPTTEKQGRIHTSTATVAVLPEAEEIDIDIKPTDL
                     RIDTFCASGPGGQCVNTTYSAIRITHLPTGLVVTCQDQKSQHQNKEKAMQILRSRLLQ
                     KLEDDRRATTDNQRRKQIGTGDRSEKIRTYNFPQDRLTDHRLQESWHGLDKIMNGEIE
                     DIIYRLQQYEKEMAQNVNH"
     gene            complement(33023..33328)
                     /gene="rpmE"
                     /locus_tag="PK960_00635"
     CDS             complement(33023..33328)
                     /gene="rpmE"
                     /locus_tag="PK960_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPD07715.1"
                     /translation="MKKNLHPTYYPNAKVTCACGNTFTTGSTLSEIKTEICSACHPFY
                     TGKQKLVDTARRVEKFTAKIESKTTIRSKKTKHAARNKAREIKAQAAKEKLEKELNA"
     gene            33548..34291
                     /gene="rpsB"
                     /locus_tag="PK960_00640"
     CDS             33548..34291
                     /gene="rpsB"
                     /locus_tag="PK960_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPD07716.1"
                     /translation="MSQTFTLADLLAAGLHFGHKTSKWNPKMKPYIYGVKQGIHIIDL
                     TKTQEQLEKALAFITKEVAAGKNILLVGTKDQVKEPLRRVAEATDMPYVTEHWLGGTL
                     TNFFIIKNSIRKYKDILDKKRTGQLSKYTKKEQLEFDRELARLEVGVGGLINLIKVPD
                     VIFIWDIKREKTALSEALKRHIPIVAICDTNTDPTGIAYPIPANDDAIKGVELILNAV
                     EEAILEGKKQREAAREIKQSVAAKKSITT"
     gene            34342..35151
                     /gene="tsf"
                     /locus_tag="PK960_00645"
     CDS             34342..35151
                     /gene="tsf"
                     /locus_tag="PK960_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPD07717.1"
                     /translation="MEINMEMIKELREQSGAGIVDCKAALVEASGDLAMALEILRKKG
                     IAKAGKRADQATNQGIIKAWVDSDEHWGCLLELKTETDFVARNDKFQQLADDILKAAV
                     NNKVQDLGSLLALTINGQIIQEAIDNLSGIMGEKIVLGNYASLISQGTVASYLHGGGT
                     IGVLVAINKPSASELAKDLAMQIAATNPKYLTPEQVPASEIEKEKAIYREQLRREGKP
                     AEIIEKIVDSKLSHYFAEVCLIKQEFIKDESKTIEGLLAGAIIEGYYRFQL"
     gene            35164..36012
                     /gene="ricT"
                     /locus_tag="PK960_00650"
     CDS             35164..36012
                     /gene="ricT"
                     /locus_tag="PK960_00650"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPD07718.1"
                     /translation="MKLALVQIYPWERASLCEIGEWSLSIGDRLLVATDTGTEVGRLL
                     GFKYLSEEQAQAWAGHTKQILGLANADDCRLIDSFDKSAALNECQRFIDQCQLEMKLV
                     DAAISADGHRLTFAFTAEGRVDFRELVRLLSHHFNCQIRLQQIGTRDEAKIFGGRGIC
                     GRPLCCSTFLTELSSVTSEVADIQGVSHRGSERISGICGRLMCCLAYEAEGYKEMAQK
                     LPEVGSPWEIEGENGIVIDRNILKGTIMVELSDKQSGGSIVIEVDPLKPSENKSSMAH
                     RRWKKH"
     gene            36024..36200
                     /locus_tag="PK960_00655"
     CDS             36024..36200
                     /locus_tag="PK960_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012765834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPD07719.1"
                     /translation="MIKIDQTKCIGCGLCATLCPENFQLSSDTFKAEVIKEEPSACLS
                     EAIENCPVQAISRD"
     gene            36219..37514
                     /locus_tag="PK960_00660"
     CDS             36219..37514
                     /locus_tag="PK960_00660"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPD07720.1"
                     /translation="MFTLEEVKLLSLPLGPGVYQFYNHSNQIIYIGKAVNLRRRVLSY
                     WQNYRQLTPAKQQLVDSIAHIKWHETESEIEALLLEANLIKKYQPKYNIVWRDDKRYI
                     YIAISADAFPRVYLTRKLAPSGQFFGPFTSVQAARETLKAIRKIWPYRSCRAMPKRVC
                     LYYQLGQCPGPCQGLISKAAYQKIIHQLSLFLQGKKQLVVKKIRTELDKYTQELNKLK
                     SEDRTAIEQKIISLNWQLQQINKVLATSCVLSLEERYADDVVELGKILGLTKPPRRIE
                     GYDVSNLYGAQAVGSLVVFVDGEASPSDYKRFKLGGNSVNLGDVPLLREMFRRRLAHD
                     DWLKPDLIIIDGARAQVNAVYNLLKQAGWDVPVIGLAKDQGLRSARALDKIYFPGESQ
                     PLQLSLNSPALFLLKRVRNEAHRFAISFHRHIRRKVFWE"
     gene            37974..38471
                     /locus_tag="PK960_00665"
     CDS             37974..38471
                     /locus_tag="PK960_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07721.1"
                     /translation="MPSSPDGLKKTAGIVLLVGMLYALRSLKSKSVVIAREATKLSSS
                     MPFLRAIEGIEKDGTTRQLVIIQRYHHHEEKEELLGGADNQQELLNELEIDDPPSDMT
                     RLLYRQFVVATAMMIVGVNRVVVRDLESPVLYTSKRVTLIPPNVKFKVLIELAKKLNY
                     EVSIK"
     gene            complement(38566..39171)
                     /gene="recR"
                     /locus_tag="PK960_00670"
     CDS             complement(38566..39171)
                     /gene="recR"
                     /locus_tag="PK960_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPD07722.1"
                     /translation="MAYPPLVQQLIQLLSQLPSVGPKTAERYIFYLLEQKPELIEPLA
                     HHLEQLSQTLTTCSECGAITLSSPCPICRDPQRSATLLCVVATNQDLEAIETTGSYPG
                     RYFVLGGYLDTLNGIKPTDLRLAALLQYLQKNKDIKEIILALNPTIEGETTALYIQKL
                     LKGKWPGRISRLARGLATGTNLEYTDPLTLANALKFRQEVK"
     gene            complement(39177..39455)
                     /locus_tag="PK960_00675"
     CDS             complement(39177..39455)
                     /locus_tag="PK960_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPD07723.1"
                     /translation="MFNKLKQFKDLRDQAKTMQSALATEKVTAEKNGVVIEMNGNMEV
                     LSLKINQDLNKEEMANTCKDVVNDAIKKTQRLMAKKMQDMGGLANLGL"
     gene            complement(39467..40849)
                     /gene="dnaB"
                     /locus_tag="PK960_00680"
     CDS             complement(39467..40849)
                     /gene="dnaB"
                     /locus_tag="PK960_00680"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPD07724.1"
                     /translation="MDRSSETIKMPPQNQEAETSLLGCLLIDKTAIVKVIDIIKPDDF
                     YNDTNGLIFGAMVSLYQDHEPIDLVNLADRLKAKNQLDTIGGRSYLTSLLQGVATAAN
                     VVSYAEIIKHKSTLRRLITAASEINELGYDEDDDLDQVLDQAEQKLFSVSHQYLRQSF
                     QPISTLLPEAFDRIDELHKQSGRLRGLATGFIDLDRLLSGLQKSDLVILASRPSVGKT
                     SFALDLARQAAIRSRAKVGIFSLEMSKEQLVDRLICAEANVSLWKMRTGQLSDREEDD
                     DFGRISYAMGALADSPIYIDDSPNCNVMEIRTKCRRLQSEHGLDLVIIDYLQLMEGRS
                     QRSDNRVQEVAEITRSLKGLARELNVPVVALSQLSRAVEQTKPAIPKLAHLRESGSIE
                     QDADVVMFIYRKAADRNYDIDSLNDFERHVAEIHIAKHRNGPTGKINLFFDEDTVNFK
                     NLEKSDLEAE"
     gene            40998..41750
                     /locus_tag="PK960_00685"
     CDS             40998..41750
                     /locus_tag="PK960_00685"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPD07725.1"
                     /translation="MWRRSFYLIVFLLSLSFSPALANNLPDQDSDGVPDQDEINIYHT
                     DPYSRDTDQDGYSDREELLHGFSPHNSKPVTLADNDQDGDGLSDFWELRFKTNLVVAD
                     TDGDGINDGQEIEAATNPREAGKVKLPMRLDIVLSRQELTYWLDGVKFKQFPVSTGKP
                     STPTPTGHFKEVAKSLKAWSKPYGLWMPYWMGLDHGQFGIHELPIWPNGYREGANHLG
                     KPVSHGCIRLGIGPAKYLYDRVDIGTEVNIYK"
     gene            41756..42142
                     /locus_tag="PK960_00690"
     CDS             41756..42142
                     /locus_tag="PK960_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPD07726.1"
                     /translation="MLYSEKVMDHFQHPRNQGALDNPSAIGQAGNPVCGDIMKIYLQV
                     NDDGIIEDIKFETLGCAAAIAVSSALTELVKGKTLDEVYKITKDKVVNDLGGLPPAKI
                     HCSMLGVDALHAAIDNYRQKIAIGKK"
     gene            42190..42441
                     /locus_tag="PK960_00695"
     CDS             42190..42441
                     /locus_tag="PK960_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPD07727.1"
                     /translation="MIDIKKISWWPELEKIIAQEIRPSLQLDGGDIKLVDFDPTTGVL
                     RVELQGHCAHCPMMELTLKEGIEAELITRLPEIKQVVVI"
     gene            42446..43675
                     /locus_tag="PK960_00700"
     CDS             42446..43675
                     /locus_tag="PK960_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPD07728.1"
                     /translation="MKVYLDYSATTPVDPQVYKAMRPYFSQIFGNPASLHSYGQAARV
                     AVDQARQKVADFLGAQPQEIIFTSGATESDNLAIMGVIAGFKANNPQLKPHIIISSIE
                     HDAVRQPGRQLEQLGVEVSWLPVNSQGIVEVNSLEKLIKPNTILVSVIYVNSETGARQ
                     PLREIGSLLEKINKTREAPGQQAGAHSTKRLPHIYFHTDATQAINYFNCQVDYLHCDL
                     LSFSGHKIYGPKGIGVLYVRSRTPLQPIQLGGHQENNRRSGTLNVPGIVGLAKALELV
                     AKQQEEEVRRIGDLRQHLVDGIMRNIPDVVLNTELSQASPSHAHISIMRAEGESVLLA
                     LDLAGIAVSTGSACASSSLEASSTLLAMGISPEVSHYSLRFTLGRWTTLAEIDYVLEQ
                     LPPIVERLRHLSANLTI"
     gene            43672..44085
                     /locus_tag="PK960_00705"
     CDS             43672..44085
                     /locus_tag="PK960_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07729.1"
                     /translation="MKLFLSKIINYFYLAGQAWQVVWSDIWHELTGRTLIIINGILWI
                     GSWAIATILYFLGGSKLLILHYNIHFGIDLIGRPAMIYWLPLGLTVAIVVNSFLSLAH
                     YPVPRQLRLVILVGTLLVSVLVNVALGSLLFINFY"
     gene            44090..45142
                     /gene="mraY"
                     /locus_tag="PK960_00710"
     CDS             44090..45142
                     /gene="mraY"
                     /locus_tag="PK960_00710"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPD07730.1"
                     /translation="MADFYIIRLLLLSAGAFIFTMAWTPLLTNFLYRHKLGKTIRNNG
                     STPIFSKLHAAKSGTPTMGGVLIWVTVLIFALGLALVHWLWPNSWLGELNFLSRSQTY
                     LPLGILVFAALVGLFDDWLDVRGLGTKGGGGLTVKVRLLIYTLIALVGALWFYFKLGW
                     DTFHVPFLGNFNLGWWYIPVFVIIIVSTAFSVNETDGLDGLAGGTLLGSFMVYSVLSF
                     LTGRYDLAVFCGVIGGALLAFLWFNINPARFYMGDTGSMSLGITLGVIAMLTNTALIL
                     PVIGLIFVIESLSVIIQVVSKKLRHGRKVFLSAPIHHHLEAKGWPEPKIVMRFWVIAA
                     VSGALGICLFLLDHSW"
     gene            45152..46345
                     /locus_tag="PK960_00715"
     CDS             45152..46345
                     /locus_tag="PK960_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HPD07731.1"
                     /translation="MVRANLKKNVTITRSRSAVKVPSKFVHHRPDRLLVVAVILLCCI
                     GIFCLASASSVVAYTSHGDAYYWVKKQLFSLIIGGLGFYILSCIDYHRWQKWGGVVLI
                     ITIVFLLTIFTPLARERAGSVAWLNIFGFSLQPSEFVKLSLTLYLSALMAKLALSREE
                     GEKKRLNTIFWLVLIAVSALILAQPDLGTLVIIAATSILIYLVGGGSWKWVIRISLVG
                     LILFVAFILIKGGYQKDRFLCVLKPGYSPDKSCYQVNQSLIAVGSGGFWGRGLGESRQ
                     KYLYLPEVQNDFIFAVIAEELGWWFSLIILGLYYLIFYRGYLVARLAPDRYGQILAIG
                     IVGGLLIQVLINLGGILNLLPMTGVTLPLISYGGSSLMAVLFGLGILANISSHTSLSS
                     SKLTS"
     gene            46342..47421
                     /locus_tag="PK960_00720"
     CDS             46342..47421
                     /locus_tag="PK960_00720"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HPD07732.1"
                     /translation="MKLIKRRRNLVILTGGGTAGSVTPLLAIAEVIGNNYKEISFLWF
                     GTTRGPERQLAEEAKIPFVTVAAGKLRRYWSWHNIIDVFKIIGGFFQGEYLLIKYRPK
                     VVVSAGSFVSVPIAWAAKLLGIPLVIEQLDYRPGLANKLMAKAADCILVTFAKSLQDY
                     GSKAIWLGAPVSSQLNNTEPDDYSLPWKISRKRPIVLIMGGGTGALALNEAVYREIGN
                     LTKLADIIHITGWGKGQEVKQGGYFSAEFLNHRQMIVAYKIANIVVARAGLGTMIELA
                     ALAKPTILVPLPNSHQLDNARVAAQAGAVIMLEEKDLVDYGLTEIVRGLLFDTQKQTN
                     LVSNWPKVIKIASEAEILKIFSRYL"
     gene            47424..48878
                     /gene="murC"
                     /locus_tag="PK960_00725"
     CDS             47424..48878
                     /gene="murC"
                     /locus_tag="PK960_00725"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPD07733.1"
                     /translation="MDWSLVKKIYFIGIKGVGMTMLAQFLHRRGYEVVGSDIADTFMT
                     DQVLVKEGIRILRGFGDNEYLRAADLVIYSTAYNQENPEVAWALANRPTLTYAEALGE
                     VFNQHYGIAVAGSHGKTTVSAWLGFTLWQSGLSPNVLVGSNVPQFGGSALLGKSSLMV
                     IEADEYQNKLYYLQPKMVLLNNIDYDHIDFYPTVQDYEQAFIDFIGKVPTDGYLIANY
                     DDPTVRRLAPIYCRGQLVSYALNYAADYVAYDLQLVNGRQYFKVKLGVDLEELGEVHK
                     KIDKLMVENHGDLGDFSTALWGRHNISNSLAVLAASVELGVPLYKIREGIAEFLGTSR
                     RLEILGNFQGAILIDDYAHHPTEIKATLEALRERYPGAKIRVFFHPHTFSRTAAFLND
                     FALSFMMADEVGILRIYSSAREHNGDISGQDLAQKIFFQRQAAGFKNQVPYFSTMEEL
                     TEFLRQTAGKDQVILLMGAGDIFRVGQALLTQTK"
     gene            48880..49821
                     /gene="murB"
                     /locus_tag="PK960_00730"
     CDS             48880..49821
                     /gene="murB"
                     /locus_tag="PK960_00730"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPD07734.1"
                     /translation="MKKPFNIQNNIPLAPYTTYKIGGPAQFFVRVSSLEELSLAIRWA
                     KAEGLSLRFLGGGSNLLISDRGVNGLVITLAAHEPNFLESGVKVGAGYGLSKLVNDSV
                     KQGLQGLEWAAGIPGQVGGAVRGNAGAFGGWIANNITAVEVYNTQAERLEVFKKEDCH
                     FDYRDSIFKRRPELLIWSATFSLTLADRESLEKIAKEHRHYRREHHPTLPSAGCVFKN
                     VPVNEVMGRAPELWSAAQLARAVSGQEVAAGFIIAQAGLAGYAVGPVQVSTQHSNFLV
                     NRGGATAQEVHQLISYIQETIKRVYHLELVPELAFWE"
     gene            49913..50920
                     /locus_tag="PK960_00735"
     CDS             49913..50920
                     /locus_tag="PK960_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPD07735.1"
                     /translation="MFIKRIAIDLGTTYTLVHLPKKGIVVNEPSVVAVSMTDKKILAV
                     GNEAKDMLGRTPDTIMAIRPLKDGVIADYHTTEIMLRYFINKALGGIHLFRPEVMVAV
                     PASITSTERRAVIDATLAAGARAAYLIKEPVAAAIGANIPVGSASGHMIIDVGGGTAE
                     MAVISLGGVVTATSVRVGGNKIDSSIIEYVRRKYNLAIGERTAEEIKIGVGSALYLTE
                     KLFMKIKGRDILTGLPKIITVSSDDVTEAIQRELEAIVGAAKNILRETPPELAADIME
                     KGAVLSGGTALLRNLDQLISRTTDVPVIIAEDPWASVVKGTGIALENLDSYKRSIFAT
                     K"
     gene            50922..52055
                     /locus_tag="PK960_00740"
     CDS             50922..52055
                     /locus_tag="PK960_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPD07736.1"
                     /translation="MIIGIDASRANRAHKTGVEWYAYHIIKNLVEIDPDDDYILYSDK
                     PLEGDLLQLCKVHSNCKQTVLHWPLRYFWTLGRLSWEMIVRPPDVLFVPAHTLPLFSR
                     AKMVNTIHDVAFMVYPQFYSTLDRKYLQWSTRFALSRAEQIITVSNFTKQEIMRYFLM
                     AQEDKISVVYHGYDRELYHPITHQSVDNPVLDYYHLERPYFIYVGRLESKKNIIGLLN
                     AFRNFCQERPQDKTKLVLVGKRSRDYAPINNLLADPIIKERAKELGWVPEKDLPALLR
                     QSLAFIYPSWYEGFGLPIIQAMACGTPVIAARVGSLPEIGSQVALYFDPADQGDLLEK
                     MKQLTDDSALRHQLSQAGLKRAAQFSWSQAAAQTLEILKKSHG"
     gene            52048..54147
                     /locus_tag="PK960_00745"
     CDS             52048..54147
                     /locus_tag="PK960_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPD07737.1"
                     /translation="MARFRLIHIRKILGGLIILVLAIFIGSKVKADELYKIALLPSDS
                     YYNNQWYLPKIKAPIAWEEQRESPDIVIAIIDSGVQIDHPDLKDNIWINNREIPNNQK
                     DDDGNGFIDDVNGWDFVNNVPDPNPKFQPGFTEEGVFHGTIIAGVAAAMGNNAAGVAG
                     VTWRAQIMPLKALDDRGLGDTFRIIQAIDYAINNGADIINLSFVGYNYSQSLAEAIER
                     AYKAGVIVVAAAGNEESGGYGHSLDKLPMYPACLDGGKNMVIGVASTDALDQKTKFSS
                     YGARCVDIAAPGVSIFSTVVYSPNRQINGRLLNKYYDGYWSGTSVSVAMVSGALALIM
                     EKNPSLKRDQVVQVLLNNADDITRLNMDYLGQLGRGRLNIASAINDASLQLSNKVSRF
                     LVAPASSSSQPVKILDSDNHPVGEFYAFGTNFPYGLRVASGDLDGDGAPEIVVVPDEK
                     GQSAVKIFNYKGKLVGQFIAFSKDFSAGLRVAVGNVDSDRAGEIIVGTGYGATNEVRV
                     FTAQGVLKYKFRPYDANFKGGVSVATGDINGDGIMEIITGAGKGGGPHVRIFTGKGQV
                     LGQFMAYESNFHGGIEVFAGNFDYGAKANRDEIVVSPRAGRLPEVRIFDGRAKMISSF
                     LAYNNNFQKGLAVALSDIDRDGLMEIMTAAGPGGGPHVRVFEVDGELRKSWYVYNPLS
                     SSGVNIAPLNLSRQFIN"
     gene            54155..55180
                     /locus_tag="PK960_00750"
     CDS             54155..55180
                     /locus_tag="PK960_00750"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPD07738.1"
                     /translation="MAVQKIFDKKNVLVTGGAGFIGSHLCDELIKTNKVICIDNFLGG
                     SERNIDHLLANPDFIFIRHDMSEPIDLLTIPELNRFKVKFQGIQEIYNLACPMSPIDF
                     MQNRIASLLANSVAVINALEMARQYQAKFMHFSSSVVYGTREVHGGNKVAETDLGIVD
                     QISERACYDEGKRFAETIVSTYRDYYGLNAKMVRLFRIYGPRMPINQGHMIPDFVVNA
                     LDNKDLIINGDENFTSSLCYVSDAIDGVLKLMQSEFSGPVNIGSEVDVKLKEVAEKII
                     QKTGSSSKIVYADRLLFMTELNLPDTTLAREQLGWMPVVGLDHGLDKTIEDLRSRKAL
                     KELPTNW"
     gene            55188..55850
                     /locus_tag="PK960_00755"
     CDS             55188..55850
                     /locus_tag="PK960_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD07739.1"
                     /translation="MKIVCVIPAWNEAKTIVNVIQDVKKHVDEVIVVDDGSEDKTANL
                     AHSAGATVLVHRLNRGQGAALRTGTIYALNHGADIIVHFDADGQFTAQDIPKVIAPIV
                     QGEADIVFGSRFLGAEHNMPPFKKRVVLPLARLFNLVFLGVRVRDPQNGFRALSAKAA
                     RQLNWQQDRMAHCSEILWLTRKAKLRYQEVGVKVKYNDFGQKMSGGWKIIGDLFLGRF
                     LK"
     gene            55860..56216
                     /locus_tag="PK960_00760"
     CDS             55860..56216
                     /locus_tag="PK960_00760"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HPD07740.1"
                     /translation="MLQKVLALIIIAFFLAKLLWQQHERKLSWTEFFFWLLFWLLSAV
                     AILMLDDIDRLVAKLGFSGNGIEVLLYVGFALLLYLMFRLRLKLEKIEHQLTTLVSAM
                     AQQKPLESSQDNKEDK"
     gene            56225..57385
                     /locus_tag="PK960_00765"
     CDS             56225..57385
                     /locus_tag="PK960_00765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPD07741.1"
                     /translation="MKIIHLVCTFPPYQGGMGNSVKDMVERLALTDPSCDITVVCPRY
                     GLREVAPVSTLYSVRRCSSWLRCGNAAVIKGLKKIWQSADIVHLHYPFYGTDALVWYF
                     KKRHPHTKLVIHYHMDPQADGWKGRIFKIWQKLFLKQLLTQAEAITCASLDYWRHSSA
                     GRIDGLSDKLFIIPFTVDTNRFQPRSKNQTLLETYGLKSEVPIILMVGGLDRAHYFKG
                     VPVLLQALAQLKVKLGKNYCQGIIVGEGDLRNKYNNLAQTLGLSHDVLFIGQVSQDNL
                     PFIYNLADLFVLPSINSCEAFGLVLLEAMASGLPCLASNLPGVREVIDPPRNGLLVEP
                     NNADDLANKISQLLADDLKLKEMAQASRDRAVKYFASSTPSPLAPIYHQLTE"
     gene            57402..58580
                     /locus_tag="PK960_00770"
     CDS             57402..58580
                     /locus_tag="PK960_00770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD07742.1"
                     /translation="MKILLANNVFSPYERGGAEEVVCYLAESWSEIGHEIMVVSLAPR
                     GHKKIRKQDNSYQIVYWPSNYYRLSNLFKWQRFVWHLPDWLGLRHHWKWRKVLKKFQP
                     DLVVLNNLTGLGFEVHHLCQRLGIPSVQVIHDVQYLQPSGLLLLGEEKLLANNWLMRC
                     YRNLTAYWLKPAKVLISPSYWLKDFYCHYNWLGDNCWLKLANPLKITLSSSYRLPDQL
                     RQAIFVGQLTPAKGIGWLAGQWSDFNKLLLAAGLPEANLTIIGDGPLFSSLNREASLD
                     EHLRLMGRLAHEAVLDQLKIADVLIMPSFCYENWPTILLEAASMGCLALVVSHGGAQE
                     LAEALGYLTFEAGNLNSLFNAWSQLSLAAAKLTYWPAGEGIKMITPRDYLTEILKNIN
                     "
     gene            complement(58577..60496)
                     /locus_tag="PK960_00775"
     CDS             complement(58577..60496)
                     /locus_tag="PK960_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07743.1"
                     /translation="MKILTYLKQPPTILLIINLISIWGTIVYWLYGLNWLGEIITLML
                     TVLTLFIIYRKQPQSKTEKPPQKKSSIISWFILIAIIITFIYLLKQKTTLPLLSPWTQ
                     VNWYFWLICWLIAAAGLITIKANKKISTLTIIAWWLLIYGIASSLYYSAYGFDPFIHQ
                     AAEKTIVAQGQINPKTPYYLGQYSLVITIHHLTGLAISSIERWLLPLLIACFLPWFIM
                     EWFRQNHHSDQSGRLAVWLWPIFTWPIFIVTVPQNCAYFFLIITIIAATWPNKSSPLI
                     WSSALASLVCHPLAGLPAITVALLSSLSYVQRRWQTSLKWLASTFTALVLPLSLWLTN
                     WQQTKKWQLAKPPINNLLKELTNFFQFSHLWPNQEHWLLNLIYGWQAWQPLVAMGLIL
                     TGWHLLRKNKTTWASILKLLNLSLMIAIILTSFVPFNFIIDYERYNYLTRFLWLAIII
                     NLPAALMTFSLLAHKWLNSHYKKLIWPIIIALTTLITFSLYLTYPRDDRYSHAQGLST
                     SQADFQAVQIIEQRANDNYIVLANQQVSAAALAQYGFYPGKHQRYLQNNLFYYPIPTS
                     GILYTYYLKMVNDYPSRQTIAEAAKLAGVHQAYFVLNNYWYGFEKIAKEAATEADEII
                     KIANGAILIFVYNNL"
     gene            60495..62219
                     /locus_tag="PK960_00780"
     CDS             60495..62219
                     /locus_tag="PK960_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07744.1"
                     /translation="MMKLLKINKSSVAISVFLIIIGGLFFIGTSAYSYCIQRPGFVKW
                     SSPDETANYYFSKLWAETGSLIAQSPIDVPEGVLVRPRSFKVVDNTLRPVSFVGLPLL
                     YGFLARLSSVDILPYLTPFFAVLGLLAFFGIVRRLFNLPTAIIGTTLAAFFPVYWYFT
                     AKSMFHNVLFAACGLIACYCGLRALDRLRLARHQSVSYFPALIWLAVSGLAFGWAIAT
                     RTSELLWLIPIALILVIAYFKEIGWYRWLWWASWSWIGMLPAVYWNIILYDHWYGGGY
                     PTMSQSIATISQTSLSLFNFHNLADKFTVLWQTIFHFGFWPKQSLTMFQHYVIEMFPW
                     LMPLALIGILVFIFHRRQWKKDKWLYLFLWILASVFLVLYYGSWTFNDNPDASHFTIG
                     NSYTRYWLPFYLGALPWAAEGLWQLANFLFRRRLVKPIVTLAVIGISCWSLHFTAVGS
                     EEGLKFTAERQVADYHLWSEVLSRTEDDSIIITLYQDKLFFPERQVLVGLFDNNVMNR
                     YYGNLLKKWPVYYFSFRFSDDALEYLNNRRLPPFGYKLEKIWDYNEFALYRLQPIPEL
                     PLNVSPQQ"
     gene            62224..63393
                     /locus_tag="PK960_00785"
     CDS             62224..63393
                     /locus_tag="PK960_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPD07745.1"
                     /translation="MKQRAENYNIGIDARLYGPLGRGLGRYIEEVVKRVVALDPTNQY
                     ILFLSPRSFNDCQIEQANVQKVIMPQRWYSLAEQLAWPRLIYHYHLDMLHVPHFNAPL
                     ICPCPLVITIHDLILTQFPSRRASTLSPVVYWFKNLAYHLVIARAVKKAETIITISEF
                     TKKDIIQQLAVPPDKIEVIYNGLTTISSGSSYDFKADRDVLLRYNIKAPYFLYVGSAY
                     PHKNLEWLVQLFIRWSKKHPGYQLVLVGEHDYFYERLIRFIADLPEKESRGEIICPGF
                     VPDEALAALYRQAALYVFPSRYEGFGLPPLEAMSYGCPVLSSDSSCLPEILGSAVSYF
                     KDNDMDSALRAMEKILHNKNYRERLIALGLEQIKKYSWDNCAQETLKIYKKVLYH"
     gene            63403..65646
                     /locus_tag="PK960_00790"
     CDS             63403..65646
                     /locus_tag="PK960_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HPD07746.1"
                     /translation="MRQTSKQYNNDDIPRSDFLLDLRQQIIDEPLVKKHLSKRKKRQF
                     HWPKISKFTLWGRGGVRVKIKWPTIKRPRLSVKISRPASLRPLWHLIIIFLVIVIPLK
                     LIVYIEDLRGMRVRVVNASSEGLANLETAGQLLMNEDFTSASSAFTQASASFAHAKEN
                     ILGVQSWLWQLAALVPSEQMHLGAKAPYIIEAGNLASKLGTDFTILAQHIVDKIGNND
                     PLSLMRQINVDSSQALADSVALNNILNKIKPASLPGSYREPLENWQAKLQTISNSLEQ
                     LQFWSAASLDLLGEKMDRRYLLVFQNNAEARATGGFIGSYALLDVSRGQIKNLEIPGG
                     GSYDTDGNLRRFVNPPAPLRLVSTYWKFWDANWWPDWPTSARELEWFLLESNGPSVDG
                     VIALTPDVLETLLTITGPIDLTKDYGLVITADNFRPLVQDYVENKVSGPKEPKKIIGD
                     LAVAILEKLPQSLNDQASIVKLLEQMSTELATKHILLYFNNEAIEQRWELTGLAGQLA
                     PLTPGQDYLQVVNTNIAGGKTDLKIRQTIEERIEIQEDGSVINDVTIIREHTGERGDG
                     LYGVRNVDWLRLYVPEGSELLAADGFSAPESHFFKPQDVTAEDMPAIKLGEGSAQIEP
                     SSGTLVYNELGRTVFANWSMVDPGETVRLNFRYRLPFNVLEVGGNTDWLSKVKAYLGL
                     GRIGKYQIIIENQAGQSPTTWKVMWSWPAKWSLLWSSVDSIKANTWQVNDIVTKQAIG
                     LLFKVDN"
     gene            65654..67576
                     /locus_tag="PK960_00795"
     CDS             65654..67576
                     /locus_tag="PK960_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07747.1"
                     /translation="MPKKNISKKSSIDYFEQMFQAGQPVIKKVEHKSHKSKEELRERE
                     VEDSLSEIYQDDAGRNIDVQKISVRPRRWWLKIILFLLYSAIIIAIGYLGYRYYISYQ
                     AKDDLFTVVVEADKNLTAGREFAYTISYHNKGRLALEDVEIKAEWPKSFIFRGADPAT
                     TSLNVWHIGHVPAEGSGQIVVKGILLNKIGENNQVHLTSSFRPANLSSAFVINTTYNV
                     ILTDSVLAVGISAPDVLAIGRDNEVIISYQEKEESLVDNVQLIMNDVDWAEVKLFDGD
                     QEIQSTGNFRWTLPTPSSELKSLKLVIKPSEQESRLEILSFRLETNIGEQSYLIDSRD
                     FDCHLVNSRLNLLLKINDSNADSGVFAGTPLNYRIDYVNQGDTTLKDVKIIASLEGAW
                     LDWSSLKDSQQGQIKGQTIIWSKKEIPSLALLKPGASGSIHFSINVKDWQNGDKNDSG
                     EIRSYAYYQTGDKVIEKPTDEEKSNTIINQLNSDLSLTESVVYFNEDNIAVGSGPVPM
                     VVGETTNLKVYWRISNSLHDLRDVEVIGELPDYVDWGGKNHSSVGELSYDADSRQVKW
                     HIDRLPANSQEVSGDFSISVTPRPDQKNQIIVVLPGSTITAIDNVTQARLEFTTQAKT
                     SRLEDDTIVQTDGLVQ"
     gene            67596..68150
                     /locus_tag="PK960_00800"
     CDS             67596..68150
                     /locus_tag="PK960_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HPD07748.1"
                     /translation="MNQFILNQIADFQKAIDFFIKDIGTLRTGRAHPGLLDNIVIQAY
                     GDYYPINALANISVPESRSLVIIPWDKTVLKDIEKGLIAANLGLGIVNEGDKIRLTIP
                     PLNEENRRDLVKRLNDKMEEARIVLRQAREAVKNKIEAAMVAKEISEDDKFRFIKELD
                     EFLAQKNEELKALRDKKEEEIMTI"
     gene            68147..69268
                     /gene="rseP"
                     /locus_tag="PK960_00805"
     CDS             68147..69268
                     /gene="rseP"
                     /locus_tag="PK960_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPD07749.1"
                     /translation="MTVVIFLGILGILVLVHELGHFLAARAIGVGVEEFGLGFPPRLV
                     GWFKNRHGHWKLLKKKHHHPRHTIFSINLIPLGGFVKLRGEDGVSRPRDHTSFANKTV
                     GERAIIIISGVLMNVVLPMIIFSLGFMIGWPQATEDLDPGARVKNPELMIAEVVPNSP
                     ASRAGLEPGDAIIEINGQPARSAAELRQINAAAAGQEVNYKIRRSSEILELSIAAEQL
                     ASNNNDYSLGVALVESGLVSYAWPRAIWEGIKLTALSLKDIAVGFWQLFTGLFKGQDI
                     SGDVAGPVGIAVLVGQISHLGWIYLLQFIALLSLNLAFINILPFPALDGGRLFFIGLE
                     AITRHPAKAELEALIHSIGFVLLLALVALITIKDIAKLF"
     gene            69293..69919
                     /locus_tag="PK960_00810"
     CDS             69293..69919
                     /locus_tag="PK960_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07750.1"
                     /translation="MRFSRSFKLLINFIRLTLAGAIVLAIILHSWANLLIIILAVILT
                     FLPEWVEKKYNIYIPLGFEFAVVLLIYASLFLGEVGRFYERFWWWDVFLHSFSSIAFS
                     CLSFIALFFLFKADKIKGRPIWIAIFSFCFSVAISTLWEIFEFFMDQTFGFNMQKSGL
                     MDTMGDLISASGSALLVATIGYFYLKSDQKTILHRWLDWIIKKIFAGR"
     gene            complement(70052..>70736)
                     /locus_tag="PK960_00815"
     CDS             complement(70052..>70736)
                     /locus_tag="PK960_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07751.1"
                     /translation="INKALLLNNDLMGLYNQNNASSSKLYLYNLVVDKKEIQNKDCNN
                     DACKFTIYKFENTDVPILIMSYKNIDNILTETIEKNDPLSYSIVDSLISVSKEETQFD
                     FTSSQLQSSALECGVNNDSDYFDKLVSKFNNTPKIVYTFKYKENSQDDGIFKVTVLPN
                     KPGYTSLDQFKKDFDICSAGGDAYPTLVNKNWLLFVSSCGSGFDDGSGRVHGCEEIRK
                     IVEPSLKLN"
BASE COUNT        20368 a        11278 c        15908 g        23182 t
ORIGIN      
        1 gtcagtctat tgaattatct aaaaagtatg ggctttaccg tcagagatat tgtggctgta
       61 agtttagtat agataagaat aatataaaat gattatatac ctttaactga taatctttgt
      121 ctttgatgag ttttattgac gatttttact tcttctatct ttttatttta gcgtattatt
      181 cttttaacca agttggctag tcttctagtc gatctcgtgt tataatacta ttaattaatc
      241 ttttaactat ggacctacaa ttttattttg agcaggctat tgctagaaaa gcttcggata
      301 ttcatctggt gggagatagc gcaccctatt tgcggattga cggacaattg gtgaaattaa
      361 gtgaagagat aattgatgct aaatggttag ctgaggcagt ttatggattt ttggatcaac
      421 gtttggtgga aaggttggaa aataataaag agttagacgt ttctaagacc attggcggta
      481 gtcattttcg tattaatttg cattaccagc ggggagttat agggttaaca gcgaggctaa
      541 tttctagcgt cattcctgac cctgaattca ttggtcttaa tgagaatttg cagcatttag
      601 cttttttaaa tgatggtttt gtgttggtag ttggtccttg tggagctggt aaatctacta
      661 ccctggctag tttattggga atcattaatc gggaacggca aggtaaaatt attaccattg
      721 aggacccaat tgaatatatt tttcaaaatg accattgtct tattgagcaa cgggaaattg
      781 gaattgatac caatgatttt gcagtagctc ttaaatatgt gttgcggcaa gaccccaatg
      841 taattatggt tggtgagatg cgggataatg agacgataga agcagcttta actgcagcag
      901 aaactggtca tttagttttt tctaccttac atactgcgac ggcagctgaa tccttatcta
      961 gggttgttag ctcctttccg gcccatcaac aaagtcaaat tgctaccaag ttatctttgt
     1021 gtcttcgggc agttattgct caacgtttat tgccagcagc ttcggggggc cgggtagcag
     1081 ctcgagaaat tatgtttaat actccggcta ttgccaattt actgcgtagt caaaaattag
     1141 agcagattcc ttctattatc caaacttcat ctcgtgaagg catgatcacc tttaaccgag
     1201 ccattgagga gctgttgcta tctggcaaaa ttacttccga gatagcaaaa atttatgaac
     1261 gagctgattc aggggcctca tactggcgtt aattattttt gatatgagtt ctactgttaa
     1321 aagaaccccg atacttattg gcctattatc tttggctagt ttagagatca ttaacttacg
     1381 ggtttatttt atttattggt taccacttat aatggttata gtaatgtttg tagtgtcatg
     1441 gtttggttta gctcatcata gggggacata caaaataata gcctggttaa ttccggctct
     1501 taatttttta gcttggtgtg gttttttaac tattattaac agttctttcg ttcgccagct
     1561 gtttataatt attgtagcag taagccagtg gtggtattgg tatgaaggat tgaaagagat
     1621 accggctagt caattaaggc taggtgaatg gtggcgaacg gttaatgttt taaactgttg
     1681 taccgtttgg gtaatagcgt ctgtccttta cggttggcag tcttttttgg gttggccgat
     1741 ttggtggccc tggttgatgt ggtttttgtg tttggccatt ataattatag gtgattatta
     1801 tgtttctgat cttaaactgt cagtccattg gcccatggta gttgctacat ggctaatttt
     1861 aggtcaatta tttgtaataa tttatttttt accaagttct aattttgttt tagcttattt
     1921 attattacta gcctattata ttttcggaca gttaaatcgt aataatttat tgcaaataaa
     1981 tactaaaaaa cgattacgct accagataat agctttgatg ttgggcttaa ttttagttat
     2041 tagcacagct aaatggtttt aattaagata tcttatgaaa tttaaaacta cccacaaaaa
     2101 actttcccct aatttttggt ggtggctttt agcggttgtg gttaatttag ttttagtatt
     2161 aatttggttt aaatactttt ttaccttagg ggaagctgat ttattggcct caatggcttt
     2221 atctggtcaa ggtatggggg tgcctatgag tagtaatcga ataacggacc taaaacatac
     2281 tgtagctatt gattataatg aagccgctag tttatggcca tcattgtcgg caagcgctat
     2341 ccagattttt aaatctcagg ataagatcca gattacaatt aatggtaaga cagagaatgg
     2401 ctataatcta gataaaccat tagctagttt ttgggtagtt actaatgatg gttggttagt
     2461 aacttcgcaa gattttagtg actggtcaag tcttatagct gtgactaatg atcaccgaac
     2521 gcatactatt agtaaaaaat taattgatcc tctaactggt cttgtttttt atcgtctaga
     2581 gggagctaat aatttggtgc cccttaaatt gggagaaaac ggttggagtc gtccgggtca
     2641 attattttat aacctaaatg gacggggtgg ttttcagaca agttggctag aaaaagttaa
     2701 ttataatgaa gctgattcat tgactataaa tagtgatagc cttaggcaac gttggcaatt
     2761 ttcaactatc tggccagatt cagctttatt taatggggcg ggagaactat ttggtttggt
     2821 taatggagag aacgaattta ttccggttaa ttatataaaa tctggtctca caagtttatt
     2881 aagtggtgat gcttttaagc gggttagctt gggcttggaa tatatagacc ttaatcagtt
     2941 gtcgcttaac aatcattatg gtttgttgat tactcaagta attaaagata gcccagcggc
     3001 taaggccgga gttaaagccg gtgatatttt attaagtatt gatgagataa atctagatgg
     3061 tgaagtttct attaatgaaa tcattcaaaa ttatcgtcca catgaccgag ccagtttagt
     3121 tatccaacgg gctgggcaga atcatcaatt aaattgtcaa ttttaatata tgccaataaa
     3181 tacaattgct agtttacccc agggggaagg ccgcaaaatc tttttgcggt tagacctgaa
     3241 cgtgccgtta gagggggggc acataaagga agattacaaa attagagcag cccgctattc
     3301 cattcaggaa ttattaaagc gtgattttag gcttatttta gccactcatt taggagagcc
     3361 aatggttaat ggtcaactaa ttccgggggc cctagagaca ctatccgtta aaccaattgt
     3421 gacttatcta agcgaattat tgtcagaaac agtagaattt gtgccaggtc aagattttaa
     3481 aaaaattagg caacagatag ataaatcacc tagtaggcta tttatgctag aaaatttacg
     3541 tttttggcct ggagaattga ccaatgatcc acattttgcc aaagatttag ctagtttggc
     3601 ggatttttat gtcaacgatg cttttgctgt tagtcatcgg caacaggcat cggttgcagc
     3661 aattaaagat tttttgccaa gttttgctgg ttatttattg gagcaagaag tggtaaattt
     3721 gtctcaagcc ctaagtccac atcaacccct tattgtggta gttggtggag ctaagattaa
     3781 tactaaagta aattttttgc gtcatcttgg agagaaggcg gcaaagattc ttttaggtgg
     3841 ggcattagcc aataattttt tagctgctcg gggctataat gttggccaat cgttagtaga
     3901 taaggagagc attgctttat caggggaaat tataaaggag ctaggagata aattgatatt
     3961 acctctagat gtaattgtta aaaatcaagg gcgtccagta gtccgttcgg ttaatcaggt
     4021 ggcggatgat gataatattt tagatatagg gccagctact atgcaagcat ttagtcggga
     4081 aattaagtca gctaagacgc tggtttggaa tgggccaatg ggaaaatttg aagaagacca
     4141 ttaccagcat gggactttat tcgtggcgcg agaaattgct tggcgctcac gtggtccagc
     4201 ttttggtata gttggcggtg gtgaaacagt agaagcctta gagatgacta aaatgactaa
     4261 ttatgtagat tgggtttcga caggtggtgg agcaatgctg gcttttttag gcggtgagcc
     4321 tatgcctggt ttagaaaaaa ttatttatta attgtttatt actattatgg caaaaattgt
     4381 tcaagtggca gcgcgggaaa ttttagattc acggggtgat cctacagtag aggctaaaat
     4441 tattttagat aatggtttaa tggctaaggc tagtgtgccg tcgggggctt caactggagt
     4501 tcatgaggct cacgagttaa gagatggtga cctagaacgt tatggcggca agggtgtact
     4561 tagggccata gccaatattt cagaggttat agggcctaag attataggag aagatattac
     4621 tgaactttct aaaattgatc atctgatgtt ggaattagat ggtacagcta ataaagaaaa
     4681 attaggcgct aatgctattt tggctatatc tatggcctgc gctagggccg gagcgttagc
     4741 tactggccag gagctttatt cttacttagc cagtagctat ggtttttcta ctagcaatta
     4801 tcgtcttcca atcccaagtt ttaatatttt taatggaggc aagcatgcag ataccaatgt
     4861 tgattttcaa gaatttatga ttatcccctt agtagatact cttgtagctg aaaagattag
     4921 gttgggctct gaagtttttc aagctttagg tcggttatta aaggataatg gtctagatac
     4981 taatttagga aatgagggcg gttatgcacc caacatgcca agtagtgaga cggtaataga
     5041 ttttattatc aaggctattc aaatagctgg ctatataccc caaggacaag tcggtttggg
     5101 gttagacgtt ggctcttccg aactttatga tgaggccagt ggtcggtata tttttaagct
     5161 tgaccaatgg cagctaacag cggctgaact aattaacaag tataaagaat gggcccttaa
     5221 gtatccatta gtttctattg aagacggact cgctgaagat gattgggata attggcaaat
     5281 attaactcag gaattaggta gccagcttct tttaattggt gatgatttat ttgttacgca
     5341 ggctgagcga ctgcgggagg gggtggagaa aaaagtggct aatgctattt taattaaatt
     5401 aaatcaagtg ggtaccgtaa gtgagactat agattgcata aagttagccc agcagagcgg
     5461 ctataaaata atgatttctc atcggagcgg tgagactact gatgatttta ttgctgattt
     5521 agctgtggct gttggggcag agtatattaa aagtggttca ctagctcgag gtgagcggtt
     5581 agctaaatat aatcgtttaa tggaaattga ggctcagcta gctggaaaat tctagtgagc
     5641 tatgagtcag gttgtagctg ataataaaat gaaaaaaatt agtccagtag ttttagtact
     5701 attagatggt tggggtattg ctccgcccag tataggcaat atgttttctc gagctcactg
     5761 gccccacata tataattatt tgcaacaata cccaatagct gctttagcta gtccattagc
     5821 ttctaatgga acacgtaaag tagcttattg gcaaattggc agtggtcagg aagtagagat
     5881 gattcctgga ttaggcggag tagttgatcg gccaggatta actgaacttt tagttcaggc
     5941 tggcttgagg caagcctata taggagaaag cgaaactttt cctttagtag ttgaatattt
     6001 agctaatcac tgcctacata atttagtaga ttatcttttg gtgagtaccc cagttaataa
     6061 tgattataat aagaatgtat tatcggctag ccatttagta attgagcgag cagctcattg
     6121 ttggtggcat taccaattag attttatggt agtaagtttg gcctcgtggg ctttagttag
     6181 ccgttatggt gatgagtcta gtgtggttaa agctatagta gccatggata ggcttctacc
     6241 taaattaatt aatccgctct tgcaagccgg ggtaactatt atactaactt cagcttatgg
     6301 catcttagaa aattacctga acccactaga taataaaata aatcatggtt taagtggcaa
     6361 ccctacccct ttagtaataa ttggtcggcc atggcgtgga caaagtttgg gagaaaccga
     6421 agttataact actgacctca ctactttagc tgttattggt tctctccgtt ctattgctcc
     6481 aactatttta actattttgg gcatagagaa acctgattat atgacagatc ccttgttgcc
     6541 ggcaactgcc attaatagat gagatatgaa gttaaataaa ttatggtatg gatccattaa
     6601 taatattggc tgggcagcag tgatagtagg cctatcttct tttattagcc gttttttggg
     6661 agtttttcgg gaccatattt tagctggcca atttggagcc ggtgatgagt tggatattta
     6721 ttatgccgct tttcgggtgc ctgatttaat tttcaatcta ttagtattgg gagctttgtc
     6781 ggctggattt attcctattt ttgttagctt gttagataaa ccggaaaaag aggaagacaa
     6841 caaggcagcc tggcggtttg ctaatatagt taccaattgt ttattgatta ttcttatagg
     6901 tttatgtatt ataggtatta ttttggctcc gcagataata aaggctataa ccccaggctt
     6961 ttctggtgaa aaattatcta agacaataga gcttagtcga ttaatgtttt tatctccttt
     7021 gcttcttggc ctttcaggga tagtaggggg tattcttcag tcctttaaac gttttttcgc
     7081 ttattcttta tctcctattt tttataattt aggcattatt gctggagctt tatttttagt
     7141 accacatatt ggtttaattg gtttagcgtg gggggtagtc attggagctt tgcttcattt
     7201 attagtgcaa gttcccactt tgagacattt aggctttcgc tatcaattta tattagactg
     7261 gcgtgataaa catttacgac aaattggccg tattatgata ccccgcaccc taggattggc
     7321 tgtcagtcag cttaatttat tagcaatgac tgtgttagct tccaaattag ctagcggttc
     7381 attggcggtt tttaatttag ctaataattt acagtcgttt ccagtaggta tttttggttt
     7441 atcctttgcc gtggcagctt ttcctactat gtcagcttta gtggccgaca aagataagtt
     7501 aagggctagt ttgacagcta ctattagaca gacactattt ttagttattc cgtcaactgt
     7561 aatgattcta ggtttgcggg cccagattat tcgggttatc ttaggttctg gccgttttga
     7621 ttggcaggat actattttaa ctatgcggac gttgagttat tttgggatat ctatttttgc
     7681 tcaatcactt aatgctattt tggtgcgagt tttctatgct tacaaaaatt cagccacacc
     7741 attttggatt agtttggtat ctagcctagt taatattagt ttagcctggt tattaagcgg
     7801 gcaattaggg gtggctgggc tggcattagc tttttctgta gggagtatag ttaatcttat
     7861 tttattatgg atttttttac gcattaaggc accagtttta gatggtcggg ctatcgctga
     7921 cggtgccatt aaaatggtta ttgcagcagc catatctgga ttagcaatta tggaagccaa
     7981 aaaaattatt gctccactgg ttaacatgcg tactttttgg ggtatttttg gtcagctatg
     8041 tggtgcagca attatagggg cggtggttta tctggcaatt tgtagtttat tacgttgccc
     8101 tgaagcaaaa gaattgtgga tgatgataag tcataaatgg cctagtaaga agaagaaaat
     8161 ggtagtagac gcagaagagg ctagaggagt ataacacttg acttttaaga taaatagtta
     8221 ttaaatataa tgtctgttct ttaaaaaata taaatggagg tgatccatgt taccatatgt
     8281 atctttgtta atttggcgtc gggctagtca gccccatggt attgaaatac tgttggcctt
     8341 acggtcagca tttcaattcg aagatgccag ccgtgttcag ccacaagatt tttacagagg
     8401 ttggcaaatt ttccccagtg gtaaactggg gagtgatgag aattcgccgc ttagtgccgc
     8461 ttggtcaaaa ttgcgagatg acggcggagt cagggcgatg atcgggctaa aggctctagt
     8521 tgggagtaat cctactagat taagacaact tgatcagaat tgctttgccg tagagatcaa
     8581 ggagtcagat gattttgatg gtgagagcaa ttttttgaat ttagtgaatt tatcaccaaa
     8641 ttatatactc gtcgcctgga agtccttagc cgagattctt cattttaatg atcttgcttt
     8701 atataggcac ggcgtgccaa aaggagtaga agcaatattt agagatcagc ttgatttaat
     8761 aaaaaaatta gctgatcagg taaggaggac gggatgagta tagtagtatt ggctatggct
     8821 tttgggactg gaaaggccat caaccagccc ataaagaaac aagtagaaga gatggtggcc
     8881 ttaaagggct atgatgtttt tactcagcct gatctggccg aaattttaga actgaaggct
     8941 ggacatttat ttgtcgctaa tcagtacggt aactatatat ccaccttagc attcatgcag
     9001 caattcgttt taatggctaa ggtgtatggc tggaaaagcg tcaggctggt agcagccaga
     9061 cagcatatct ggcgctgtgc gcgcgatctg cgccatctgg caccggagat gcacgttcaa
     9121 ctttatccgg tcacggttga gtatggcccc gacactcaac tgtgggtgag atcatcgatt
     9181 gggtggtggg ccagggagat agtaataaga ttactaccct ggccaattta taaaaaaatt
     9241 tgttagaaat agggggttat catgaagata gccccccctt tatttacctt aaataagttc
     9301 attctcattt tggactatga agaaaaaata gaataaagtg ggatgcattg acttgacaga
     9361 taggaaaata agcataacaa tatagatact tactaaatga tagtaagaaa aataaaagga
     9421 ggtgtctaaa tgaaaaaaat ttagcctaga cgccggggac cccagggtcg caatttaaaa
     9481 aattgtatta gacgggctaa ttataaggct cgtatccaat taaataaccc tctaataaaa
     9541 gacaggaggc tgcttatgaa gcagaaaaat aaaattattt aattggagcc cctactagac
     9601 catgcttttc acaccttata aaggtgttaa ggaatgccat aagctgtgga aacagcaccc
     9661 ttgaggtggc taaccttaat tattttttat cttgcatagg cctggtgggg gcctttttat
     9721 tatcattgac ttgttgtaag ccatttagca taatataggg gtatgaagaa tatccgtaat
     9781 ttttgtatta ttgcccacat tgaccatggc aaatctactc tagctgatcg tttattggag
     9841 cttactggga ctattgagcc acgtaagatg actaatcaaa tactggaccg tttagattta
     9901 gagcgagagc gggggattac tattaagctt acgccagtga ggttaaagta tcgaggttat
     9961 gagcttaatt taattgacac tcctggtcat gtggatttta gctatgaggt atctcgtagt
    10021 ttagcggcag tcgagggagc gattttatta gttgatgcta ctcagggtat tcaagctcag
    10081 accctagcta atttatatct agccattgag caaaatttag ctattattcc agtagtcaac
    10141 aagattgatt tgccagctgc tgacatcgca aaaactaaag gagagattgt caaactgctt
    10201 ggctgtgcgc ctgaagaaat catttgtgtt tcagccaaaa caggtgaggg ggtacccaaa
    10261 atattagagc ggatcattga agtggtgcct gcaccgatcg gagatgagga taaacccctt
    10321 cgggccctaa tttttgattc taattatgat gaataccgcg gggtagtagc tcatattcgg
    10381 gtagttgatg gtataattcg tcggggagag aggattaaat tagtggcgac tggagcagta
    10441 agtgaagctt tggaagttgg cttttttgac cctaattatc ataaaagtca agagttacgg
    10501 gcgggagaaa tcggttatgt ggtaactaat tttcatgaag taagtgaatg ccgtgtaggt
    10561 gatactctta ccctagaaaa agaagcccag aagattatac cccttaaagg atatattgag
    10621 gttaaaccga tggtctttgc tggtgttttt cctaaagagg gtgatgcttt tgagaatttg
    10681 cgggaggcga tgggtcgtct taaattaaat gacgccgctt tagtttatga acctgaacag
    10741 tcacaggcat tagggtatgg ctttcgctgt ggttttttgg gtttgctaca tttagagatt
    10801 tttcaggaga gattaaggcg ggagtttaat cttgatctta tagtaactgt tcccagcgta
    10861 gcttataaat tatatcgccg taatggcagt gaaccagaaa tcattcgcag tcctcagcaa
    10921 ttgcctaatg gggcggagat tgattatttg gaagaaccat gggttagact agatattatc
    10981 actcctaaag agtatttagg agcagtaatg aaattagccc aagaaaaacg ggggatttat
    11041 cgtaatactg aatatcttga cgcaacccgg gtaattttac attatgagat gccgcttagt
    11101 atgattttaa ctgattttta tgataaactt aaaagcctta cttcagggta tgggtcaatt
    11161 aactatgaaa tgttagatta ccgtcgagct gaagtagtac ggttagatat tatggtggct
    11221 gaccatttag ttgagccgct ggctactatt gtttatcgtg acgaggcctt taaaagaggt
    11281 aaagaaattg ttactacttt aaaagagact ttgccgcgag agatgttcgt gataaaatta
    11341 caggcagtga ttggtggtca aataattgct gctgagcgta tttcagcttt acgcaaagat
    11401 gtgacagcca agctttacgg tggtgatgtc actcgaaagc ggaaactatt agaaaaacag
    11461 cgacaaggca agaaaaagat gatggccttg ggacgaggac aggtggatat tcctagtgaa
    11521 gtctttgtta aattattgaa acattaataa tttatatgag caagaaaaaa aagaagaaaa
    11581 ttactaaacg gcgcatcatt aatgctgtct ggattattat tagtgctata gtggtattat
    11641 ctatgatagt ttggacagtg gctattggtt tctaatttag tcttatggcg cccccccgcc
    11701 tgtggcaaat agcacctagt ttaaagaaga gtaattctga gctagcagaa ttaggtctac
    11761 cttttattat tgagcaaatt ttgtataatc gtggtctaga taaggcagat gagattcgtc
    11821 aatttttagg cctccaacca tttttagcta atctggcaga tcttagccaa ttatctttgg
    11881 ccgtagaggt cattgtgaag catattaaag ccggtaattt aattgtagtt tatggcgact
    11941 atgatgctga tggtgttact gcaagcacca ttttattaga gaccctagct atccttaaag
    12001 ctaaaacgag aatctggtta cctgatcggg taactgaggg ctatgggctt aatactgggg
    12061 ctattgatga gatagcccag tggggagcta aacttattat tactgtggat actggtattc
    12121 gcaataaaca ggaggttgct tatgctcaga agaaagggtt agaggttatt gttactgatc
    12181 atcatcgaga gtcgcctgat ccggctgaat ggccagactg tttaattatt aatccgacta
    12241 tgacgagaga acattatcct tgtaagtttt tagccggggt aggggtggct tataaattag
    12301 tacaggctat agtagagcgg tctactctct cagataatca gaaaaaaacc ctagaacgtc
    12361 gttgtctaga tctagttgct ctaggcacca ttgctgattg tgtgcctttg cttatggaga
    12421 accgtcaatt agtaaaagag ggtgttgaaa ttatgaatac taggcgacgg ataggcttgg
    12481 aagaattagc gcgagtggcg caaattagtg gagatattaa tgagtggaat attagttggc
    12541 aattggcgcc tcgtattaat gtagctggtc gactagatca tgctaattca gcttatcgtt
    12601 tattaaccac caaggatcaa gccgaagctg cccagatagc cgatgatttg aatcaaaaaa
    12661 acaatgaacg tcagcagaaa actcaagaga ttgttgattt ttgtttaagt gaattattag
    12721 aattacaagc cgatgatagt ctattaatat ttgttagccc tagacaggat ccttggccgg
    12781 agggtattat tggcttagtg gctggccgat tggcagagag gacggggaaa ccagttattg
    12841 taattactaa aggtgagcag gggtataaag gctcaggtcg cagtaatgtg gaaaatttta
    12901 gtcttattgc attattagaa gaagtagcca gtgatctaga tcgttatgga ggtcatgctg
    12961 gagcttgtgg gtttaattta aaagttcgac agctacccaa ttttttaact aatattaaga
    13021 aattagcggc taaaaaatta gctaatcttg atttacgacc caagctattt attgatggcg
    13081 tattacctat taaggatata gatatgaata ttgccgaatg gatagagaaa ttagcgccgt
    13141 ttggtcaagc taatcctgag ccaaagtttg tggcccatca tgaggtaatt gacgatgtta
    13201 gtaccgcagg tcttagccag caacatctta agttacgttt tggtaattat tgggcgttgg
    13261 cctttggaca ggctgaagat tatagcgatt tgcgccccgg tcaacttgtg gatttagtat
    13321 ataatttagg gattaatcgg tataataatc atgcggagct acagctaaaa attattgatt
    13381 tacgtcgaat aaataattga ggcatcaaat tgtatgacta aaaaatttgt tcatcaaagt
    13441 ctaattagct atacagatgg tggagcgcga ggcaatcccg gtccagctgg tattggagta
    13501 gtcttaaaga cgaaaaacgg tgagacggta ggtgaatggt ctaaattttt aggaataact
    13561 actaacaatc aggcagaata tagggcttta ttattggccc tagaagaggc cagatctttg
    13621 ggggtagaag aattagagtg ccggctagat agtgaattgg tggtaaaaca actgcgaggt
    13681 gaatataagg ttaaacatcc tgatcttaaa ccattatttg cccaagtatt aagtttgatt
    13741 tctagtttta aacatgttag ttttaactat atcccccgga ctttaaataa agaagctgat
    13801 catttagcta atcaggccat gaatcaggaa atgaatagga aatataagga cacatagctc
    13861 agttggttag agcgtacggt tcacatccgt aaggtcacag gttcaaatcc tgttgtgtcc
    13921 actttatttg taggtagtta gtaaatttag gaattcagtt aaaattaaat taaagaagct
    13981 ttaattatag ttgactaatg aacttatagg ggtataatat ccttagacca gccagtcagc
    14041 gggcagttgc tgtttagcaa taaacagagg aaagtccgga ctccctggat actaaagtat
    14101 ccagtcgtga taacgggtaa cacccgccgc atcagttgtg agactgatgt agggaaagtg
    14161 ccacagagac tatactagcg actaagtcgt caaaggtgaa aagtcagctg gtgttgcgct
    14221 taccgcagct acagctgtcc ctagtcagta atggctaggc gtggtaaacc ctatctggag
    14281 caagaacaaa ctaacagcta actgttagac aaagtggttc gcaggagctt ttgagtaatc
    14341 aaaagccaag acaaataact gccgtttcgt tttttacgaa atacaaaatc cggcttatag
    14401 ctgatggctg gtctaaaagt atttttatga aacgtagtga actatttttt tcttttatcc
    14461 ttttaccaat tgattttata atgattgttt tggctggtat ctcggcctat tatctgcgga
    14521 ttagtgatgt tgctactggt gttcgaccta ttatttataa tttaccgtat aaagattttt
    14581 tatctttact attgttaata gcgttagctt ggctggtagt atttgcttta gctaatttat
    14641 atcaagcgcg tcctatcaaa tggtttgatg aattaagacg gatatttttt gcttgcctag
    14701 ctggttttgg cttgattgtt atttatattt tcttttttcg agaatggttt tcttcacgat
    14761 ttattatcct ggttggttgt gctttagctt ggatttatgt aagcatagct cgtttgttgg
    14821 tacggtggct gcaaagatat ttatatcgct atggcattgg agtccatcgt gtggtgattg
    14881 taggacgtgg agcctcggcg gataaactaa ttaaagaatg gtttagccac cgctctcatg
    14941 gatatcaagt agtcaaacat tatgataatt ttgatttgga gacggctcaa gagctggaag
    15001 actatttaaa aattaagcca gtagatgaga ttattcaggc ggatcctaat ttgagtaaag
    15061 ctgaggttat tagattatat gattttgctg aggagcatca cttagtttat aaatatattg
    15121 ctgatttatt agacactaaa attttaaaaa ctgaagtgga tgaaattgct ggtattccgg
    15181 tagtagaggt taaaaaaact cctttagatg gctggggaaa aattgctaag cggattttcg
    15241 atcttgtttt ttccacgttt tttatcattt tatttagtcc actttattta ttaacagcaa
    15301 tagcaattaa attagattca tctggaccag ttttctttag tcggcttgat gatggttcgc
    15361 cgctatatcg tatagggcag ggtggccgta agtttcgtta ttttaagttt cgttccatgg
    15421 tacctggtac tgacagttta cgttacacag ctttagctga tcgcaatatt cgcagtgatg
    15481 gtccgatggt aaaaattaaa gatgatccac ggataacccg ggtgggcaaa tttatccgtc
    15541 ggtttagtat tgatgagctc ccggaatttt ttttagtatt tattggctct atgagtttag
    15601 tgggaccgcg accgcatctg ccggaagaag tagctaaata tgagcggcac cacaaaaaag
    15661 tgttaacagt caaaccgggc gttacggggc tagcgcaggt atctggcagg tcagatttaa
    15721 gttttgagga ggaggtgaaa ttagatgttt actatattga gaattggtca ctggctaaag
    15781 atattagtat tttattaagg actccgatgg ctgtttttcg tcatcggcag gctgaataat
    15841 atgaaggtgg ctttaataca cgaccattta gctcaagatg ggggtgctga gagagtactg
    15901 agggtattag ctgagatgta tccccaggcg gatatttata ctttgcttta tgaaaagcgg
    15961 caagcagata aatatttttc agaccggaca attaagactt catttattca acgtgtacct
    16021 ggcggggtaa aacattacca atggtttttg ccctggatgc cactggcagt agaatttttt
    16081 gatctttcag actatgattt ggttatttct gatgccagcg cttttgctaa aggagtaatc
    16141 actggcatta atgtgccgca tatttgttat tgtcacactc ccaccagata tctgtgggac
    16201 tatacccatc gttacattgc cgagcttaaa tataataagt ttattaaaaa agttgtttcg
    16261 ctttgtctta attatttacg agtgtgggat tatcaggcgg ccaggcgggt agattatttt
    16321 gtggctaact cttctttagt gcaaaaacgt attaaaaaat attatgactg cgagagtgaa
    16381 gtaatttatc cgccagttga ggtagataat tttaaaatgg gagaaggcca gggcgattat
    16441 ttcttaattg gtggtcgggt ggtaacctat aaacgttttg atttagcagt tgaagctttc
    16501 cgagagttag ggtggccgct taaggttttt ggtgatggta tagatttgcc gagattaaaa
    16561 aaaatggcta aagaagctca taatattgag tttttaggtc gagttagtga taaagagaga
    16621 gctgagcttt accaacattg tcgagctttt ttaaatcctc aagaggaaga ttttggcatt
    16681 acggtagtag aggcaatgtc ttgcggccgg ccagttattg cttatagtca gggtggagct
    16741 ttagagacgg tagtggaagg agagaccggg gtctttttta aggagcaaac taaggaatct
    16801 ttaatagagg ccttgcatcg ttttgagcag ctatctttta atgctgtagc cattcgtcag
    16861 catgctgtaa aattttctcg acaacgtttt gagacacaat ttcgagattt tgtagagcga
    16921 gctataaatg attttaggtc taaataatat atgaaagtgg cagtagatgc tcggccgctt
    16981 ttggctaaag aaactagtgg catacctgaa tatgttgggt ttttaattga cgctctgacg
    17041 acagcccatc cagaagtaga atggcagctt ttttatagta gttggcgaga aaaaccatgg
    17101 cagtgggagt ggctaggaga gagacctaat gtttcgtggt ttccgcttaa atgtcctaat
    17161 aagcttatta atgccgcggc gtggttaata gatcagccca agttagataa atattgtcct
    17221 gctgatcttt ggcttttgcc acattttaat tttacaccat taagcggcaa gacgccggcg
    17281 gtattaacta ttcatgattt atcatttctc cgtcaaccgg agtttttttc ttggcgtcgt
    17341 cgtttttggc atgctagcct ccacctatct cggcttgtaa acctagccaa gaaaatagtg
    17401 actatttctg agagtactaa gcgggacatt atagaattat taggggtacc tgaagataaa
    17461 attacggtta tttattcggc tgtagctcgg caatttcagc ccctagccag gggtgatcag
    17521 caattaatag actgtcatca taaatataat ttaccccagc gttttattct atcgttggcc
    17581 acctgtgaac cccgcaaaaa tttagtcagt gtgattgagg cttataatct cttacgtcgt
    17641 cgcagaacaa actatgatga ttgtcaactt attattgttg gtggccatgg gtggcgtcaa
    17701 ggtttagcga gaaaggtatg gcaagggtcg ccttatcggg aagatatacg gtggctaggt
    17761 tatttgccag ctgaggattt gccagcttta tgtaatttag ccagtgtagt agtttatcct
    17821 tctttttatg agggatttgg tttgcctgtt ctggaggcaa tgtcttgcgg ccggccggtt
    17881 attacctcgg cagttactgc tttacccgag gtgggagatg gagcagcctt gttaattgac
    17941 cccgctgatg ttaatgcctt ggctcggagt atagagcaag ttttagctaa tgaagaattg
    18001 gccaaggaat tatctcgtca ggggttgaag tgggctcagc aatttagttg ggagaaaact
    18061 gcccaagaat attggcagat tttagcaggt taatctattt ttattggggt aaaataggga
    18121 tgacaagtgg agcggttttt gttatactgg agctagtttg attattataa atccccatgc
    18181 ctcaaccgac ttttgtccat cttcatgttc atagccatta ttctttgctc gatggaatgg
    18241 gtaagattga tgagcttata gatatggcca aggagcataa tgccccagct attgctttaa
    18301 ctgaccatgg agttatgtat ggggcaattg agttttacca aaaatgtcgt aaggccggta
    18361 ttaagccaat tattggagta gaattttatg tggcccctac ttcgcgtcat gataagggtg
    18421 gtcaagcgga gcggagttat cacttaattt tattggctaa aaatgaagta ggctaccata
    18481 atttattaaa actagtaagt atcggtcact tggagggttt ttattataaa ccgcgtattg
    18541 attgggagtg tttacaggaa tatagtgggg gacttattgc tttaagtgca tgtctatcgg
    18601 gagaaattcc tcggctaatt ctggctggta aatatactaa ggcgaaagaa cgagccctgg
    18661 ctcataataa attatttggc gagggcaatt attatttgga gcttcaacac caccccaatt
    18721 tacctaatca agtagaagtt aatcgagaac ttattaggtt gtctggggag accggcattc
    18781 ccatagtggc tactaatgat gttcattatt tacggacttc agatcgtgag gtacaagatg
    18841 ttcttttgtg tttacagacg aaacataaaa aacaagatag tggccgtatg aatatgttag
    18901 atgatgatta ttcatttcgt tctcctgagg agatggctca agcttttata gatactcctg
    18961 aggctattaa caatacagtg aaaattgcgg aagcatgtaa tctagaatta ccaatgaatc
    19021 aggtattact accgcatttt gaggtgcctc aagggtttac agcccaaagt tacttacggc
    19081 aattatgttt agaggggtta gttaagcgtt atggcaagac ttatgatgaa attgaggctg
    19141 tttaccgtga gaggctggac tatgagttag acgttattga taaaatgggt ttccctgatt
    19201 attttttaat tgtagctgat tttattaatt gggccaagag tcatggtatt gtggtcggtc
    19261 caggacgtgg gagcgccgct ggttctctgg tttgttattt aattggtatt actgaacttt
    19321 gtccgatttc ttatgatttg ctctttgaac gtttcttaaa cccagaacgg atttctatgc
    19381 cagatatcga tactgatttt gctgatacca gaagagacga agtcattaat tatgttgctg
    19441 ataaatatgg ccgtgatcat gtctcgcaga ttattacctt tggtacgatg gccgcacggg
    19501 ctgctatccg tgatgtggga cgggtcctcg gggctagtta tgatttttgt gacaagctag
    19561 ctaagatggt gccgatgttt atgaagttgc ctgaggccct agagcaagtg gatgaattgc
    19621 gccagattta tcaagaaagc cctcaggcta agcaaatttt agattatgct caaaggttgg
    19681 agggtagtgc gcgtcatgcc tctactcatg cttgtggagt gttaattact aaagagccac
    19741 tgaccgaata ttctcctatt cagtatgctt cttcaacgga taggactatc gtttctcagt
    19801 attcccttca ctccattgaa gatttggggt tgcttaagat ggattttttg ggacttaaaa
    19861 atttaactat tattgaatca gctctcaaaa ttattaaagt cactaaggga gtagaaatag
    19921 atattaacag gattccccta gacgatgagc ggacctttga attatttcaa aacggggaaa
    19981 ctaccggcgt atttcaattt gaatcatcag gcatgaagag gtatttacga gatttaaagc
    20041 caactaattt agaagatatt attgccatgg tagctttata tcgtccaggt ccaatggaat
    20101 ggattcctga ttatatcgcc gggaagcatg gtcgtaagaa attacaatat ttacatccta
    20161 aattagaatc aatattagct aaaacttatg gagtagcgat ttatcaagag caagtgatgg
    20221 aaattgcccg aagtttggct ggttttacaa tgggacaagc cgatgtctta cgtaaagcgg
    20281 taggcaagaa aattcctaaa ttattagctg aacagaaaga aaaatttatc actggctgta
    20341 tggccaatgg tattagtaaa accttagctc aaaagatatt ttcttttatt gaaccattcg
    20401 cgggttatgg ttttaatcgg tctcatgccg cttgttatgc aatggtagcc tatgagacag
    20461 catatttaaa agctaattat ccaacagctt ttatggctgc cttgctggcg gctgatcagc
    20521 agaatagcga ccgcgtggct attgaaattg aagaatgtga gcggatgggc attgaagttt
    20581 gtcctccaga tattaaccaa tcttttgatt cttttactgt agttgatgaa aatttggtag
    20641 accaccccac gattcgtttt ggtcttctag caattaaaaa tgttggggag catatagcta
    20701 aagttattag ggacgaacgt aaacgcggtg gaccttatca agatatgggt gattttttac
    20761 gccgtattaa tgacaaagat ttaaatcgta aatctttaga aagtctcatt aaggccggtg
    20821 cttttgatag ttggctagac cggcgtcagc ttttagaaaa tattgaagtc ttattaaagt
    20881 ataacaaaga agctagccag aatagcttaa atgggcagta tagtttattt gccggggttg
    20941 atggggtaaa agaccctcct ttatatttaa gaccagcacc accagctgac cgtcaaacca
    21001 aattaagctg ggagcgcgaa ttgttaggta tgtatattac tgagcatcct tttgtagatt
    21061 atcatcggga attgaaatca gtgatagtgc ctattaaaaa tttagcggct aaagccccgg
    21121 gggagatagt aactatcggt ggtatggtag ttagtagcaa gaaaattatc acccgaggaa
    21181 gtcaatcaat gttatttgtc aagatagaag atgacaccgg caatctagag gttatagtct
    21241 ttcctaaatt attagaagtt acgcgtgaag tgtggcaagc aggtcaacca atactttgcc
    21301 ggggaaagat ttctgataaa gataatgaac gcaaggtggt agtggacaaa gctattgttt
    21361 tgaatttaga tcatataagt gatgacttag ctgcttttcg tcagtcagcc gctaatatga
    21421 agccaggttt ttgggcagta aaaccagtat cttataaagt aaaatcggca gatttattat
    21481 caaagtcacc agaagcagta catttaattt tacggcaatc tataagccag gcccagcttt
    21541 ttaaattaaa agaaatttta ttacattatc ctgggcccca gcaagtttat ctagagattg
    21601 attttaatgg agaaaaacgg aagatagcca ccggagttca agtaaaatct ggaccggaac
    21661 tattagctga acttaaagct gaatttgccg aaagcattca attttagtta taataagaat
    21721 aatatttaac tttattttat gtctgcacaa aaaacatcaa tagcacctaa atctaggggt
    21781 agcggagcaa gaaggccagc tggccgtcca ccaaaggtta gaccattggg tcggccatcc
    21841 aaatcttcca ttgctaggaa tgttcctaat aaggttaaaa ctaaaaccca tgaagtgaag
    21901 gtacccaaac ccaaagtagt tgtagtgact aatcagcctt tagttagtga agaagaattt
    21961 tcagtttggc aggcagcccg ggaggaagag cctaaaaatg aagaattttc agttgtgcct
    22021 aatgagaatg aagatattaa catatcatca aaaccattag agccgcctga gttacccgac
    22081 aaccatcaac catcaactcg tttataccgt aatttagcta ttttttttat cattcttatt
    22141 ggttttgtaa taatggtgat gttttattat tcatttatta aagttaagat tattttatat
    22201 ccgtcggcca gtacacaaca acagaatgcc accatttatt tgacggatat taccccagta
    22261 tctaatggtt atctgccagc taaatttgtt gaacaatcta cagaatatcg ggaaacattt
    22321 aaggctactg gagagcgaca attacctaca gcaggtaaat tagttggtca agtccgatta
    22381 attaataaaa caggtaaaga ccaaccgctg gtggcgacta ctagattatt aactagtagc
    22441 ggagtacttt atcggctgaa aaattttgtt acagtgccgg cgggcggaga tatcaaagcg
    22501 gaagtttatg ctgatgaagt gaagccagaa aatgctattg aagccaatac tagatttacg
    22561 attcctggcc tgtggtctgg attacaagat aaaatctatg ctatagctga agagccaatt
    22621 gtttttcaaa gtaatattga acctattatt ttagattctg atctagatca ggctatagct
    22681 caagctaagg agggagccgc taaacaagct aagtctctta tatctcaggg atttcaagat
    22741 aaagctatag cttactggct agatgacact aagtgggagg taaagagtga tgggcaggtg
    22801 ggcgaagcca ggagtgaatt tacggtaacg gttaaagcga ccgtgggagc tgctattttt
    22861 gatcttcaag cgatgataga tctaataaat aataagctag acaatagttt agttattgac
    22921 gagtcttcaa tagcctatca actagttgat tggcagccac aagaacatta tgctgaatta
    22981 aatgtaagtt atactgctag tatagtagct actcgagctg accagttaat tgataaacgt
    23041 gccctagttg gactcaccca tgaggagtta gaagattatt tatctaaaca accgggccta
    23101 gcaacttata aaattatttg gcagcctcat tttttgaaaa aggttccagg tactgataaa
    23161 attattctac aacttgctca ataagaggtt aaacatccaa gttaataact ttactagcgt
    23221 gagtttgaat aaaatttttg cgaggcatta cttcgtcgcc cattagcatc tcaaaaactt
    23281 cattggccat agcggcatcc tctacagtaa cttgttttaa aattctacaa tctggcttca
    23341 ttgtggtttg ccagagttgt tctgggttca tttctcccaa gcctttatag cgttgaatat
    23401 ggactttagc taaaccttta ttttgaattg cttcatctgg ttcgattttt gattcagccg
    23461 cttcatcatt gatgatatta gcattattac tgcctaaatc attaatgatt ttatccttct
    23521 cttcatcgga gtaagcataa aaaatagagt tgcctttctt aatttggtaa agagggggtt
    23581 gagcaatata aagatagcca ccagttacca gaggattaaa gtgtctaaaa aataaagtaa
    23641 gtagcaaggt tctaatatga gctccatcta catcggcatc agtcataata ataatgcggt
    23701 gatagcgtaa tttggtaatg tcgaattggt catcaatgtt agtgcccagg gcaatgacta
    23761 tattttttat ttcattattt tctagcattt tatctaatct agcccgttca acattaagta
    23821 ttttaccgcg gagaggcagg atagcctgaa aacggcgatc acgaccttgt ttagcactac
    23881 cgccagcact gtctccctcg acaataaata attcacattc ttcagcccta tgactagagc
    23941 agtcagctag tttacctggg agggtagcac cttcaagggc gctttttcgt aagatagcag
    24001 tgcgggcagt tcgagcggct actcgagctt gggctgacaa taaacatttg ttaataattt
    24061 gttcagcatc tttgggatgc tcttctaaaa acgctccaag agtctcgcta caaacttgtt
    24121 ccacatagcc ttttacttcg gcatttccta gtttactttt agtttgtcct tcaaattgtg
    24181 gattggttaa tttaacgcta ataatagcaa ctaatccttc tcgtacatca tcaccggtta
    24241 aattttcttc tttatctttt aataatccct tagatcgggc atagttattg agaaccctag
    24301 taagagcgct gcgaaaacca attatatgag tgccaccttc agtattaata atgttattgg
    24361 caaaagccaa aattatttca ttatagccat cattgtattg caaggctaat tctattttga
    24421 cattattaat ttctttttct gcataaaata tggtcttgtt tttaacttga ttattacggt
    24481 ttaaattttt gatatatgat ttaatgccac cctcaaaata gaaattataa tctttgtggc
    24541 gatggcctgg tcggcgatca tgtaatttta cggtaactcc tttagtaaga taagcttgct
    24601 gacggcaatg atctaaaatt gttccccatt taaattcaat ggtatcaaat atggtggcat
    24661 ctggtctaaa ggtaatagtt gttccatgtt tattggaatc agtttgaccg atttttttta
    24721 ccggatttaa gggtttgcca tattgatatt cttgctccca aatttctccg ttccgataaa
    24781 ctgtagcttt aagataggaa cttaaagcat taactactga tactccaact ccatgaaggc
    24841 cgccagaaat tttgtaggta ttgccaccaa atttaccacc agcatgcagt ttggtcatta
    24901 cagtttctag agctgatacc ccagttgttt tatggatatc aactggtatg cctcggccat
    24961 tatcgctaat ttctactaga ttatctggta gcagtgatat ctctaccata tcggcatagt
    25021 cagccataac ctcatcaaag ctgttatcag ctacttccca gattaaatga tgtaagccaa
    25081 tactcgaagt tgagccaata tacattcctg gtctcttgcg aactggttct aagccctcta
    25141 gtacagttat ggtctcagca ttatattcag tagtagcttt tttaaaattt tgattcataa
    25201 attcattctt acataaaaaa tatagcccta cggctagcct atattgttat tattgtagca
    25261 aaaaaaataa ggattggcaa ggctgtaatt acctaattat cttgttttta gagttattaa
    25321 ttgctacaat acacctagct aaatattaaa atttgtgaaa aaaaatcttt ttaataataa
    25381 attactggaa gaatcttctg gagataaacc taaataccac tcatcgttat ctgacaagtg
    25441 gttatggctg attgctggat tactttgtat aatttttgta attatagttt tttgtttaat
    25501 aatgacgtgg aatcaaaaac caccttcggc aataggttca aggggtactg agcctgagtc
    25561 aaaacagcca gaggagattg ctttaccggt cgtgactgag tctttgacta ataatatagc
    25621 ccgagaggga tcgaattttc aggctgacca gctaatattt agtcaacttt accaacaacc
    25681 aaccactagt tggccggttg aaattctagc taataaggct caatctatta ctttgccaat
    25741 taatgttaaa aaggatgtgg ctaattatta tgaaataaaa cgttattata atttagagcc
    25801 aattattagc caattaaata aagagggatc agtgttagta gataatccct ttggcgatga
    25861 aaaacaagat ttttttgatt tttatcattc agctacagag aatcattggc cgttactttt
    25921 aactagtgat gttatttttt actattatca aaacaatatt aaagaagttt ataaagcggt
    25981 tgaagcaggc ttttatgatg atttatggca aattagcaat ggtcttttta aaatagcgaa
    26041 tcaacgttat caggcagcaa ttagccaatc actgaagggt aatgatccat tgatagaagc
    26101 ttatcgtcta aatgcccaat attgggcagt agcccttaaa ttattggctc ctaaagataa
    26161 tcagattagt aatgatggaa ttactagtaa ttccatgatt tttcggacag gtgatgacca
    26221 aatatataaa ctaccagtta ttgatgaaga tttacgtcgt gaagttgatc aagaggtaag
    26281 tttaatacaa gcagctagtg gagtaaaaat ttcacctttg tttggctatg agatggatta
    26341 tggtctattt aaacctgacg agaactatca agatggagga cgtttggcta attttttatt
    26401 agttagtaaa tggttaaatt actcctggcc actttactat cgtgaggccg gttgtacaaa
    26461 atgcccgctg gatattgatg attggcgagt taattttttg gcggccaatg tattagctag
    26521 agaccttaat agtgatcagg aatttaaaaa ttattgggct aagatttata aaattattag
    26581 ttatttttcg ggtttacgcg atgatctaac ttatgttcat tatattgcag cgcaaaaaga
    26641 aattcttggc gatcaaagcc tagaagcagt agctcgtctt gatgaagcta gtcgtaatca
    26701 agttttagat cgtctaagag tggctatttt aggaaataaa tttttaccta ctaacggtgg
    26761 gtgggatcgt cagcaaccgg ataattggcc taaaattggt ttgagggttt tgcaggatcg
    26821 ttattggcca ggacagttta ttgattggca aaccgtaggc cctaaactag atgtttttgt
    26881 tagtcaacaa actactacag attggcaagt caatcaattt tggtctagta tggaaatgat
    26941 taaagaatgg ttacctaaaa ttttatctgc taattcagag tgggtgagac cagcctggtg
    27001 gaaagctcaa catttatctt tagctaaatt ttatctcctt aattggcaat taccagcgga
    27061 ccaacgccgt attttattac ctagcgctaa tagcttgttt caaaatgatg gtgtgaccga
    27121 gaatattgtt tctatagata ctgctagttt gccagccatt gatcggcaga ttgctacagc
    27181 taaaatgttg cagttggccc tagtaaaact acaggctagt aatttatctg ctgatcaaaa
    27241 attgatggat actattaata ctttggagat gattcgggat ttagctgtta agcaagctaa
    27301 aggagaagag tggactaaag aagagtcaac agcaatcaat aatttttttc aatcggctat
    27361 agtgagatct gggagtaaac aactagaccc atcgcaaaaa gatagtcctt atctacaaaa
    27421 aataaaagcc agaatagaca tcaaaatgat caatggtcag ttatatatat taattggtcc
    27481 aatcattatg ccctagaggg attttaatca taaaagatat gcctacgaag aatcaagcct
    27541 ccaataaagt tttgcccgac cagataactg acgatgatgt ttctttaaaa aaattgtctt
    27601 ttggtctgtg gtatatgcgt catcgccgtc tatttcttaa tattattatt gttgtactaa
    27661 ttattttttc agccagtggt tggatttata gtttttatgt tttaggccat tattttattt
    27721 ttgatcgaca aaaagatcaa caattattgt cggagatggc tcaaactagt gtgaatcttt
    27781 tggctggccg taccaaccag ccgttatcta ttagtgatgt taaagttctt ggttcaggac
    27841 ctaataattt attagctgaa atttataatc ctaatcccag gacagcagtc ttttttaatt
    27901 atgcctttgt gttaaatggt cagccaattg ttcaaagaga tggctttgta ttaccaggag
    27961 agaaaaaata tcttgtggct ctaaatcaaa atattcccgt tggtgcttcg gtttatttaa
    28021 agattgatca agctcggtgg cagagattag atgcccgtca aattcctgat tggcctgcct
    28081 ttttagctga gcgtaatcaa ttagttattg ctgaccggca aattaattta gctgataatt
    28141 ctaatcggtt aattagtcaa gtgaaatttc gtatagaaaa tcattcagcc tatggttact
    28201 ggtcggtgcc catattaatt atagcttatc gggatggaaa tttagttgat gttaatgagt
    28261 tagtattatc taaactacga agtggcgagg ttagaaatat tgttttttcc tggcctggtc
    28321 ttaaagatgt tgattatata gagataatac cagaggttaa ttttttagat caggaaaact
    28381 atttgcccat cgacactgat taatttatgc atttacggtt tagccaatat tggcggtata
    28441 atgattggcc agtgacactg gccatcttat tgttattagc tattggctta agccaaattt
    28501 atggagtagc tctaggacgc ggtggtacta gcttagtggc tttttataaa caaatagttt
    28561 ttgtcatcgt tggtttaggt ggctatatac ttttatctgt ggtagattat catcgttggc
    28621 ggagctatgc taggtatttg tatattctgg cagttatttt attaattact gtattattat
    28681 ttggtagttc tattcatggg actaagggat ggtttaatat tttagggcta ggtattcagc
    28741 cagttgaatt agtgaaagtc attttaatta tttgcttagc tagctatttt tcaggccgga
    28801 ctattcggct taaccaatca aagcagttct taataagtgg actattaacc tttattttaa
    28861 cttttctggt gttattacaa ccagatcttg gttcagcttt acttttagtt attatttggt
    28921 tatttttgat tttagcagct ggttttaatc gtaaatatct atattgggta attattgctg
    28981 gtattatagc ggtggtagtg ggctggctgt ttttatttac agatattcag aaaaatcgtt
    29041 ttcgttcttt ttttaatcct aattataatg tctctggcca aagctataat attaatcagg
    29101 cgatgatcgc tgtcggctcg gggagattgt tggggcgggg attggggttt ggttcccaga
    29161 cacaattaaa atttttgccg gaagctcaaa cggattttat ttttgccgtt attgctgaag
    29221 agcggggttt tttaggcgcg ggattagtat tattattatt tgctgttatt atctatcgta
    29281 tcttaacggc tttgcctaaa gttaaagatg attttggttg ctttgtcatt gttggtgcta
    29341 ttggtctttt agctactgaa gtttttgtta atctagctat gaatattggg ttattaccaa
    29401 tagtgggatt agctttgcca ctggttagtt atggtggcag ttctttaata tccaccctat
    29461 tattactagg gttggtaaat agtatcatta ttcggtctaa aatttaattg tatgaaaatt
    29521 ccagatttta accctgagac tataattcat ggacggctaa tgaaaaagct ggagcgacag
    29581 gggttgcgta atgattcaat ttatcccatt cctgagggaa tggagcgcgt ctggcaggtt
    29641 tttattgatg gtcaaaaagt tgatcattat tctcacttag ccgcgaagtc tcaatattgc
    29701 ttgatgaata taggggtaag tcttgataaa ggtcaatatg ttactttaca agaagcagat
    29761 agcagtggag ccgttattat cccttttgta attattcaga atgaaaaact tgtctattta
    29821 aataaactaa ataaaaaaaa taactattta gtttatgccg gcttgattag acagtggcga
    29881 ctgcggcagg gcggttgggt gtggaatgta ccccgtggtt atgccaattt aggggaaaat
    29941 tccaagaaaa cagccgctcg agagttaatg gaagaaaccg gtctagctgg taagttattt
    30001 ttattgcctg gtcagccact taatcctaat agtacctatt ttgaaactgg ccataatggc
    30061 ggcgtcatta tttatgctat aaatttggga ccagatttag cagattggca aaataataaa
    30121 gtaacattac gccatcgtcc tcggccaaca ggaatagatg aaacaattaa ggcttgccgt
    30181 tttttcccgt tagctcgcct gtctcaacta ggtgatatgt tttcggtggc tgctgaatca
    30241 cgattgcgct attatcttga aaatcttaga tgatacctaa aaggatttta gtatataaat
    30301 tgacaatttt ttcttcatta cttatactgg ctatacatta acctttagtt aagtattttt
    30361 tattatgaaa agtgaattaa gattagaagc taagaccaga gaattgggtc gtcagtcact
    30421 caaaaaatta cgagcggcgg gatttattcc ggcaatagtt tatggaccgc agaccgataa
    30481 tttacccttg cagattaaaa aatctgaagc attaaaaatt tatgaacagg gtggtgaatc
    30541 cagtcttatt actttaaata ttgacggtca agacgaacgc caagtaatta ttaaaaatat
    30601 cagtcaatcc agtgtaacca atcatttact tcatattgat tttttccaag ttaatatggc
    30661 tgaaaaatta accaccgaag ttgagcttaa ttttgtagga gaagcgccgg cagttaaaca
    30721 gttgggagga gtggtggtta aaaattttga tgctgttgaa attgagtgtt tacctaaaga
    30781 cttggtatct catttggatg ttgatatatc tggccttaca gaattaggct ctcatattac
    30841 ttttgctgat ttgaagttac ctaaagggat agcattgtct agcaagcacg agatgaatga
    30901 gattattgtg gcagttgagg agccgcgggc ggtagcggct gaagctgaag taactgaaga
    30961 ggttagtgct gaagaagttc caagcgagac agcaccagag gcagaagtta gtggtgagga
    31021 gcaaaaataa ttttaattat gccaccagcc aaagcgaggc tggccgtcta aatcgcaaaa
    31081 agtttgttgc catttaaggt tagctacggc taatagtgat ggcagttttt gattatgatt
    31141 taaattatgt tcggtaagga cgttgatagt gtggtatggg tgatatttta tgaagcctgg
    31201 tagctcattt gctaattctt gaaataaagt gagcccttcc gggccaccca gtaaggctcg
    31261 gcgcggttca tattgtaacg atggttgctg attatattct ttcggggtta aatatggcag
    31321 gttagcaata ataataattt gttgagctga gtcaaaacta tctttaagcc aaggctctaa
    31381 taatgaaccg tggcgccaga caatattttt ttgtttaaga ctaatggcgt tatgcgtagc
    31441 tagacgtaag gcccggcggc taatatctga gccgccataa taatctttat tatttagttc
    31501 agcggctagc gtgattagaa ggcaaccgga gccagtacca atatctaacc agacagtagg
    31561 tttgtttgtt tcttgacgta ggcgggtagc tatatcaata aggcattcac tttcggggcg
    31621 ggggattaaa acagcggggg ttattttaaa tggtcggcca taaaaatatt gttcttttag
    31681 gatatacggt agaggataat ttttttggag tttagtggta attttttgta aactatgcca
    31741 attttttgta gttagtggtt gttgggcaca ggtaattagt tctgaggatg tacggttagt
    31801 aatactagca aggagccaac tggctagtgc tggttgttgc atactgagag gccacatctg
    31861 ttctaaccac tgacgagctt cagcaatagt aggggggtta atggttgacg ttttgcgcca
    31921 tctctttttc atattgttga agacggtaga taatatcttc aatttcgcca ttcataattt
    31981 tatctaagcc atgccatgat tcttgaagac gatgatcagt gaggcggtcc tggggaaaat
    32041 tataggtgcg aattttttcg ctgcggtctc cggttccaat ctgcttacgt ctttgattat
    32101 cagtagtagc gcgacggtca tcttctaatt tttgcagtaa gcgagagcgc aaaatttgca
    32161 tagctttttc tttattttgg tgctgtgatt tttggtcttg gcaggtgact actaggccgg
    32221 ttggtaggtg agtaatacga atggctgaat aggtagtatt tacacattgt ccgcccggac
    32281 cagaagcgca gaaagtgtca atacgcaggt cagtaggttt aatatcaata tctatttctt
    32341 cagcctctgg caatactgct actgtggcag ttgaagtatg gatacgtccc tgcttttcag
    32401 tggttggtac tcgttggacc cgatgggtgc cgctttcata tttaagccag ctaaagactt
    32461 tttctccagt aatgctaaaa ataatttctt tatagccgcc aatggctatg gggctagaat
    32521 ttaagatttc tagttggaaa ttatgatttt ctgcaaaccg gctatacatt ctaaataaat
    32581 cggccgcaaa taaagccgct tcatctcctc ccgctccggc tctaatttcc atgatgacat
    32641 tttttttatc acgagggtcg gcagggtgta gtgcttcttc caactgttga cttaaagtag
    32701 ttttttcaat tagtaattgt tgttgttctt cggttgccag ggcaataatg tccggatcag
    32761 tattgtctga gaggagttgg tcaatatagg ttaattgtga ttcaattttt tctaggcgag
    32821 tcgctaaaga tattaaatca gccactgccg ctcgctcttt aactaggcga ttatattctt
    32881 tagcattagc aatagtagct ggattattaa gttgagcgtc cagttgatta agttggtcac
    32941 taagttcttg tagtgtcata taaaaaacag aattaaaaaa atatcatcta aagacttaga
    33001 agataatttt ttatcaagta agctaagcgt ttaactcttt ttctaatttt tctttagctg
    33061 cctgggcttt tatctcacga gccttatttc tggcagcatg cttagttttt ttactgcgaa
    33121 ttgttgtttt agattctatt ttggccgtaa acttttcgac acggcgtgct gtatccacca
    33181 gtttttgttt accagtatag aaaggatgac aagctgaaca aatttcagtt ttaatttcgg
    33241 acaaggttga gccggtagta aaagtattgc cgcaagcaca agtcacctta gcatttggat
    33301 aataagtagg atgtaaattc ttcttcatag ttatacacaa aataaacgtc ttgactgacg
    33361 taataatatt aaactatcat aacttaagag aaaaagcaag ttagcttgac ataattattt
    33421 ttattgctta atgtaataaa taactattat tgagcttaat atttttaaat taacgactca
    33481 tgcattcatt ttttccttgc tcacttaagc tcatgagtaa agaatgcagc ggttgaaagg
    33541 aaattttatg tctcaaacat ttactttggc tgatttatta gccgctggcc tccattttgg
    33601 ccataaaact tctaaatgga atccaaaaat gaaaccatat atttatgggg tcaaacaggg
    33661 gattcatatt attgatttaa ccaaaactca agagcagttg gaaaaggcct tggcttttat
    33721 tactaaagaa gtggcagctg gtaaaaatat cttattggtt ggcactaaag atcaagttaa
    33781 agaaccactg cgtcgggtgg ctgaggccac tgatatgcct tatgttactg aacattggtt
    33841 gggtggtact ttaactaact tttttattat taaaaattcc atccgcaaat ataaagatat
    33901 tttagataaa aaaaggactg gtcaattaag taagtatact aaaaaagaac aattagagtt
    33961 tgatcgagaa ttagccaggc tagaagttgg agttgggggt ttgattaatt taataaaagt
    34021 ccctgatgtt atttttattt gggatataaa aagagaaaaa actgccttgt ctgaagcctt
    34081 aaaacggcat attcctattg tggctatttg cgatactaat actgacccga caggcattgc
    34141 ctatcctatt ccagctaatg atgacgctat taaaggagtg gaattaatcc ttaatgcggt
    34201 agaagaagct attttagagg gtaagaaaca acgggaagcg gctcgtgaga tcaagcaatc
    34261 agtagccgct aaaaaatcaa taacaactta gtcatttaat tatatagact aatttttaag
    34321 taaaattagc caataaacca tatggagatt aacatggaaa tgatcaaaga attacgagaa
    34381 cagtcaggag caggtattgt tgattgtaaa gcagcgttag tagaagctag cggtgattta
    34441 gctatggccc tagagatttt acgtaaaaag gggattgcta aagccggcaa gcgagccgac
    34501 caggctacta atcaggggat tattaaagct tgggttgatt ctgatgaaca ctggggctgt
    34561 ttattagagc taaaaacaga aactgatttt gtggcgcgta atgataaatt tcagcagcta
    34621 gcagatgata ttctaaaagc agcggttaat aataaagttc aggatttggg gtcattatta
    34681 gctctaacga taaatggtca gattattcaa gaggcaatag ataatctaag tggaatcatg
    34741 ggggagaaga tagttttagg caattatgct tctttaatta gccagggtac tgtagcctct
    34801 tatttacatg gtggaggaac aattggggtg ctggtggcta ttaacaaacc atcagctagt
    34861 gagttagcta aagatttggc catgcagatt gctgctacta atcccaaata tttaactcct
    34921 gaacaggtgc cagccagcga aatagagaag gagaaagcaa tttatcgtga acaactaagg
    34981 cgagaaggta aaccggctga gattatagaa aaaatagtag acagtaaact tagccattat
    35041 tttgctgaag tgtgtttaat aaaacaggaa tttattaaag atgaaagtaa gacaattgag
    35101 gggctgctag ctggtgctat tattgaaggt tattatcgtt ttcagcttta ataattctaa
    35161 ctcatgaaat tagcgttggt acaaatttat ccctgggaaa gagcaagttt atgtgaaata
    35221 ggtgagtggt cgctttccat tggtgatcgt ttattagtag cgacggatac tggcaccgaa
    35281 gttggccgtt tactaggttt taaatattta agtgaggagc aagctcaagc ttgggctggc
    35341 catactaaac aaattttagg tttagctaat gctgacgatt gccgtcttat tgactctttt
    35401 gataagtctg ccgctttaaa tgagtgtcag cgttttattg atcagtgcca actagagatg
    35461 aaattagttg atgcggccat ttcagcagat ggtcatcgct taacttttgc ctttactgcc
    35521 gagggtcgag ttgattttcg tgagctagtg cgcttgttga gtcatcattt taattgtcaa
    35581 attcggttac agcaaatcgg tacgcgagat gaagctaaaa tatttggcgg tcgtggtatc
    35641 tgcggtcggc cgctttgttg tagcactttt ttgacggaat tatcgtcagt tacttcagag
    35701 gtagcggata tacaaggagt gagtcatcgc ggcagtgaga gaatttctgg tatctgcggt
    35761 cggctgatgt gttgtctagc ttatgaagct gagggttata aagaaatggc tcaaaagcta
    35821 ccagaagttg gtagcccctg ggagattgag ggagaaaatg gtatagttat agatcgtaat
    35881 attttaaagg gtacaataat ggtcgagtta tctgataagc aatcgggtgg ctctattgta
    35941 attgaggttg accctttaaa gccgtctgaa aataaatcat caatggctca tagacgatgg
    36001 aagaagcatt agtcaaatag tatatgatta aaattgatca gactaaatgt attggttgtg
    36061 ggctatgtgc tactttatgt cctgagaatt ttcagcttag cagtgatact tttaaggccg
    36121 aagtcattaa ggaagagccg tcggcttgcc ttagtgaagc tatagagaat tgtccggtgc
    36181 aggctattag tcgtgattag aaaaattaga gagtgtctat gtttacctta gaagaggtta
    36241 aacttttatc tttaccattg ggaccagggg tgtatcaatt ttataatcac tctaaccaaa
    36301 ttatttatat aggtaaggct gttaatctac ggcgtcgggt cctatcctat tggcaaaatt
    36361 atcgtcagtt aactccagct aaacaacaat tagttgattc cattgctcat attaagtggc
    36421 acgaaacaga aagtgaaata gaagctttat tattggaagc taatttaata aaaaaatatc
    36481 agcctaaata taatattgtt tggcgtgatg ataaacgtta tatttatatt gctattagcg
    36541 ccgatgcctt tcctagagtt tatttgacac ggaaattagc acccagtgga caattttttg
    36601 gtccatttac tagtgttcaa gctgcacggg aaactcttaa agccattcgt aaaatttggc
    36661 cttatcgttc ttgtcgggca atgcccaaaa gggtttgttt atattaccag cttggccagt
    36721 gcccgggacc ttgtcagggg ttaattagta aagcggctta ccaaaaaatt attcatcagc
    36781 tcagcttatt tttacaaggt aaaaagcagt tagtagttaa aaagatacgg actgagctag
    36841 ataaatacac acaagaattg aataaattaa aatcagaaga taggactgcc attgaacaaa
    36901 aaattatttc attaaattgg caattgcagc agatcaataa agtattagcc actagttgtg
    36961 ttttaagttt agaagaaagg tatgctgatg atgtggtaga gctaggtaag atattgggtt
    37021 taacaaaacc gccacgacgt attgagggat atgatgtttc taatttgtat ggtgctcaag
    37081 ccgtcggttc gctagtggtt tttgttgatg gtgaagcatc accgtcagac tataaacgtt
    37141 ttaaattagg tggtaatagt gtcaatttag gtgacgtgcc cttacttcgt gaaatgttta
    37201 ggcgacgttt agctcacgac gattggttaa agccagactt aattattatt gatggcgcta
    37261 gggcccaagt aaatgcagtg tataatttat taaagcaagc tgggtgggat gttccagtta
    37321 ttggtttggc taaagaccag ggtttaaggt cggctcgagc cctggacaaa atctattttc
    37381 ctggtgagtc tcaacctttg caattatcgc ttaatagtcc agctttattt ttattaaagc
    37441 gagttcgaaa tgaagctcat cgttttgcta tcagttttca tcgacacatt agacgtaagg
    37501 tattttggga ataaaatttg ctagagttaa ttcatccgtt cataattcag tcactactat
    37561 tatttctatt gttttattga tcttaaggtg tcttttggaa aaccgccaag tgtaagcaca
    37621 ctacctcctc ttgactatta ttagaatata tgttattatt taatgtccta aaattatagg
    37681 actgtacatt aaaaataaaa aattatggag gctcaaaatg agagctattg ttttaacgat
    37741 aggtaacgtg atcagcaaaa tcggtatggc aatatacgat tttgtcttgg tgatgtttgg
    37801 tacattgcta ctgatattgg cattattgct actgatattg gcattatttt tctttacttt
    37861 tttccacaag gagtaatatt ctagactccc tgttgaagat tagaaggccc agacattact
    37921 gggccgaagt attaagaata ttatttataa taatattctt aggcatcatt tttgtgccca
    37981 gttctcctga tggcctgaaa aaaactgcgg gaattgtatt actggtgggg atgttatatg
    38041 ctcttcgctc gttgaagagc aaatctgttg ttatagccag agaagccaca aaactctcta
    38101 gctctatgcc ctttcttcgg gcaattgagg gcatagaaaa ggacggtact acaagacaat
    38161 tggtgattat tcagaggtac catcatcatg aagagaaaga agagttgttg ggcggggctg
    38221 acaatcagca ggaattatta aatgaattgg agatcgacga tcccccctct gacatgaccc
    38281 ggctattata taggcaattt gttgtggcta cggccatgat gattgtcggg gtcaataggg
    38341 tggtagtacg agaccttgag tcccccgtac tttacacctc gaagagggta acacttatcc
    38401 ccccaaatgt taaatttaaa gttttaattg agcttgccaa aaagctcaat tatgaagtgt
    38461 ctataaaata gaataaaagc tggtggcttg gcgaagaaca gaatcaagtt atcagcttca
    38521 tctcatcata aaataaaaac ctaggaccgt gttatgcggt cctttttatt ttacttcctg
    38581 tctaaatttt aaggcattag ccaaggttaa ggggtcagta tattctaggt tagtaccggt
    38641 agctaggccg cgagctaggc gactaatacg acccggccat ttacctttta ataatttttg
    38701 gatatataaa gcagtagttt cgccctcaat tgttggatta agggctaaaa taatctcttt
    38761 aatgtcttta tttttttgga gatattgtaa taaagcggct aatcgtaggt cagttggttt
    38821 tatgccgttt agagtatcta aatagcctcc cagtacaaaa taacgtcccg gataactacc
    38881 agtagtttca attgcttcta ggtcttgatt agtggcaaca acacataata aggtagcaga
    38941 acgctgaggg tctcgacaaa tagggcaggg gctagataaa gtaatagctc cacattcact
    39001 gcaggtagtt agagtttgac ttagttgttc aaggtggtgg gctaagggtt caattaattc
    39061 tggtttttgc tctaataggt agaaaatata acgttcggct gttttaggcc ccaccgatgg
    39121 taattgactt agaagttgaa ttaattgttg aactaatggc ggataggcca tattgcctat
    39181 aatcctaaat tagctaagcc acccatatct tgcatttttt tagccattaa tcgttgggtt
    39241 tttttaatag catcgttgac tacatcttta caggtattag ccatctcttc tttgtttagg
    39301 tcttgattaa tttttagaga caatacttcc atattgccat tcatttctat aaccactcca
    39361 tttttttcag cagtaacttt ttcagttgct agggcgcttt gcatagtttt ggcctggtcg
    39421 cgtaaatctt tgaattgttt taatttgtta aacataaaat taattgttat tcggcctcaa
    39481 ggtcgctttt ttctaaattt ttaaaattaa cagtatcttc atcaaaaaat aaattaattt
    39541 taccagtagg tccattgcgg tgtttggcaa tatgaatttc ggctacatgg cgctcaaaat
    39601 cattgaggct gtctatatca tagttacggt cagcagcttt acgatagata aacattacca
    39661 catcagcatc ttgttcaatc gaacctgatt cccgaagatg agccagttta ggaatcgctg
    39721 gtttagtttg ttctacggca cgggataatt gagataaagc cactaccggc acattaagct
    39781 ctcgggctag gcctttaagg gagcgagtaa tttcagctac ttcttgtacc cgattatcac
    39841 ttctttgact tcgcccttcc attagttgga gatagtcgat aatcactaaa tctaggccat
    39901 gttcagattg aaggcgacga catttagttc ggatttccat gacattacaa ttaggtgagt
    39961 catcaatgta aattggagaa tcagctaagg ctcccatagc atagctaatg cggccaaagt
    40021 catcgtcttc ctcgcggtct gaaagctggc cagtgcgcat tttccataga ctaacattgg
    40081 cttcagcgca aattaagcgg tctactagtt gttctttact catttctaaa gagaaaatac
    40141 ccactttagc tcggcttctt atggctgctt gtcttgctaa gtctagggca aatgaagttt
    40201 tgccgacgct aggacgggaa gctaaaatta ccaggtctga tttttgtaaa ccggaaagaa
    40261 gtctgtctag gtcaataaag ccggtagcca gaccgcgtag acggccactt tgtttgtgta
    40321 gttcatcaat ccggtcaaaa gcttcaggca gtaaagtgct aatcggttga aaagattgtc
    40381 ttaaatattg atgagagaca gaaaataatt tttgctcagc ttgatctaga acttggtcta
    40441 agtcatcatc ttcgtcatag cccaattcat tgatttctga ggcggcagta ataaggcggc
    40501 gaagagttga tttatgttta ataatttcgg catagcttac tacattagcg gcggtggcta
    40561 caccttgtaa tagactggta agataactgc gacctccaat agtgtctagc tgatttttag
    40621 ctttgagtct gtcggctagg ttaactaaat caattggttc atgatcctga taaagtgaaa
    40681 ccatggcgcc aaaaatgagg ccattggtat cattatagaa atcgtcaggt ttaataatat
    40741 caatcacctt aacgatagca gttttgtcaa taagaaggca accaagaagc gaggtttcag
    40801 cttcttgatt ctgaggcggc attttaattg tctcactgga tctgtccatg atgaaaaata
    40861 atttatgaga atattcttgt atttatgata caagccaaat ggttttttgt ctaggtgcgg
    40921 tggatgtcta tttaatgctt gaattagata tgttataata ttttatagtc tttttattat
    40981 tttttatatt tgacgctatg tggcgtcgct cattttatct tatagtattt ctactatcac
    41041 tatcttttag cccagcatta gctaataatt tgcccgatca agatagtgat ggtgtgcctg
    41101 accaggacga gattaatatt tatcatactg atccctatag tcgagatact gatcaagatg
    41161 gttatagcga ccgtgaggaa ttacttcatg gattttcacc tcataattct aaaccggtta
    41221 ctttagctga taatgatcaa gatggcgatg gtttaagtga tttttgggag ttgcgtttta
    41281 aaactaattt agtggttgct gataccgatg gcgatggaat aaatgatggt caagaaattg
    41341 aggcggcaac taatccgcgg gaagctggta aagttaaatt gccaatgagg ttagacattg
    41401 ttttaagccg tcaggaattg acttattggc tagatggggt taaatttaag cagtttcctg
    41461 tttcgactgg taaaccgtca acacctaccc cgactggtca ttttaaggaa gtggctaaaa
    41521 gtcttaaggc ctggtctaaa ccctatggat tatggatgcc ttattggatg ggattagatc
    41581 atggtcaatt cgggattcat gagttaccta tttggcccaa tggttatcgt gaaggagcta
    41641 atcatttagg caaaccggta tctcatggct gtattagatt aggtattgga ccagcgaagt
    41701 acctttatga tagagtggat ataggaaccg aggttaatat ttataaataa ataatatgtt
    41761 atatagtgaa aaagtaatgg atcattttca gcacccgcgt aatcaaggtg ctttagataa
    41821 tccgagtgct attgggcagg ctggcaatcc agtgtgtggt gatattatga aaatttatct
    41881 tcaggtgaat gatgatggaa ttattgaaga tattaaattt gaaactttag gttgtgctgc
    41941 ggctattgca gtttcttcag ccctgaccga actggttaaa ggtaaaactt tagatgaagt
    42001 ttataagatc actaaagata aagtagttaa tgatttagga ggattgccgc cagcgaaaat
    42061 tcattgttcg atgttgggtg ttgatgcttt acatgcggct attgataatt atcgtcaaaa
    42121 gatagctata ggtaaaaagt aaattttata accataattt ttattgattt tatttattca
    42181 aattatatta tgatagatat taaaaaaatc agttggtggc cagaattaga aaagattatt
    42241 gcgcaagaaa ttcggcccag tttgcagtta gacgggggag atattaagct agtggatttt
    42301 gaccctacaa ctggagtttt aagggtagag cttcagggac actgtgctca ttgtccgatg
    42361 atggaactta ctcttaaaga gggaattgaa gctgaactta ttacaaggtt acctgagatt
    42421 aagcaggtag tagtaattta atcttatgaa agtttattta gattatagtg ccactacgcc
    42481 agttgatccg caggtttata aggcaatgag gccttatttt agccaaattt ttggcaatcc
    42541 tgcctcgctt catagttatg gtcaggccgc tcgggtggcc gtggatcaag cgcgtcaaaa
    42601 agtagcagat tttttagggg ctcaacccca agaaattatt tttacttcag gagccacgga
    42661 gtcagataat ttagccatta tgggggttat tgctggcttt aaggctaata atcctcaact
    42721 taagcctcat attattattt ctagtattga gcatgacgcc gttcgtcagc ctggtcgcca
    42781 gctagagcaa ttgggggtgg aggttagttg gctgccagtt aatagtcagg gcatagtgga
    42841 agtcaacagc ttggaaaaat taattaaacc taatactatt ttggtctcag tcatttatgt
    42901 caatagcgaa actggggcca gacaaccgct tagagagatt ggatcattac tagaaaagat
    42961 taataagact cgagaggccc ctggacagca ggcaggagct cactcgacaa aacggttgcc
    43021 tcatatttat tttcataccg atgctactca ggcaattaat tattttaatt gccaggtgga
    43081 ttatttacac tgtgatttac ttagcttttc aggacataaa atttatgggc ccaaaggtat
    43141 tggggtatta tatgtacggt ccaggacgcc gctccaacct attcaattag gtggccatca
    43201 agaaaacaac cgccgtagtg gcactttaaa tgtcccggga attgtgggat tagctaaagc
    43261 gttagaatta gttgctaagc agcaagaaga agaggttagg cgaattggtg atttacgtca
    43321 acatttagtt gatgggatta tgagaaatat acctgacgta gttcttaata ctgagctatc
    43381 acaagcgagt ccttctcatg ctcatatttc tattatgcga gctgaggggg agtcagtact
    43441 gttagcccta gacttagctg gaattgctgt atctactggc tcagcttgtg cctctagtag
    43501 cctagaagca tctagtactt tactagctat gggtattagt cccgaggtgt cgcattatag
    43561 tttaagattt accttaggaa gatggactac cttagctgaa attgattatg ttttagagca
    43621 attaccacct attgtggagc gattacgcca tctgtctgct aatttgacga tatgaagttg
    43681 tttttgtcta aaattattaa ttatttttat ctagcaggtc aggcttggca ggtagtctgg
    43741 tccgatatat ggcatgaatt gactggacga accttaatta ttattaatgg aattttgtgg
    43801 ataggtagtt gggctatagc tactatttta tattttttag gaggtagtaa attattgatt
    43861 cttcattata atatccattt tggcattgac ttaataggcc gtccagcgat gatttattgg
    43921 ttgcctttag gtttaacagt ggctatagta gtaaatagtt ttttatcctt ggctcattat
    43981 cccgtaccaa gacaattgcg cttggtaatt ttagttggta ctttattggt atcagttttg
    44041 gttaatgtcg ctttgggtag tttattgttt attaattttt attaatttta tggctgattt
    44101 ttatatcatt cgtttattat tactatcagc tggggctttt atttttacca tggcctggac
    44161 gccgctccta actaattttt tgtatcgcca taaattaggc aagactattc gtaataatgg
    44221 tagcacccca atattttcta aattacatgc tgctaaaagt gggacaccaa cgatgggcgg
    44281 ggtactaata tgggtaacag tgctgatttt tgctttagga ctagccttgg ttcattggct
    44341 ttggcctaat tcgtggctcg gagaacttaa ttttttaagt cgtagtcaaa cttatctacc
    44401 cttgggtatc ttggtctttg ctgctctagt gggtttgttt gatgattggt tagatgttcg
    44461 tggtttaggc accaagggtg gtggtggact tacggttaaa gtaagattat taatctatac
    44521 attgatagct ttagttgggg ctttatggtt ttattttaag ctggggtggg atacttttca
    44581 tgtgccattt ttgggtaatt ttaatttagg ctggtggtat attccagttt ttgtcattat
    44641 tatagtcagt actgcttttt cggttaacga aactgatgga cttgatggtt tagcgggggg
    44701 tactttgctt gggagtttta tggtttatag tgttttgtcg tttttaactg gacgttatga
    44761 tttggcagtt ttttgtgggg taattggcgg cgctttattg gcctttcttt ggtttaatat
    44821 taacccggct cgtttttata tgggcgatac cggctctatg agtctaggca ttactttggg
    44881 ggtaatagct atgctaacca atactgcttt aattttgcca gttattggtt taatttttgt
    44941 cattgaatct ttgtcagtaa ttattcaggt tgtttctaaa aaattacgtc atggccgtaa
    45001 ggtctttttg tcggccccta ttcatcacca tctggaagct aaaggttggc ctgagcctaa
    45061 aatagtaatg cgtttttggg tcattgcggc ggtcagcggg gctttgggca tttgtttatt
    45121 tttacttgat catagttggt aaattaagac tatggtccgg gctaatctta aaaaaaatgt
    45181 aaccataacc agaagccgtt cagcagttaa agtgccgtct aaatttgtgc atcatcgtcc
    45241 cgaccgtctt ttggtcgttg ccgtaatact attgtgttgt attgggattt tttgtttagc
    45301 tagcgcctca tcagtagtag cttatactag ccatggtgat gcctattatt gggttaagaa
    45361 gcagttattt tctttaatta ttggcggtct tggtttttat attttaagtt gtattgatta
    45421 tcatcgctgg cagaaatggg gtggtgtagt attaataatt actatcgtct ttctacttac
    45481 tatttttact cctctagcta gagaacgagc tggctcagta gcgtggctta atatctttgg
    45541 tttttctctt cagccatctg aatttgtgaa gctatcatta actttatatt tatcggcttt
    45601 gatggctaag ctagctctta gccgtgaaga aggagaaaaa aaacgactta atacaatttt
    45661 ttggctggta cttatagccg ttagtgcttt aattttagcc cagccagatt taggaacctt
    45721 ggttattatt gccgctacca gtattttaat ttatctggtg ggcggtggta gctggaaatg
    45781 ggttattcgt atatctttag taggattaat actttttgtt gcctttatac ttattaaggg
    45841 tggttaccaa aaggaccgtt ttttatgtgt attaaaaccg ggctatagcc cagataaaag
    45901 ttgctaccag gttaatcagt cactgattgc tgtagggtca ggcggctttt ggggacgggg
    45961 gctgggagag agtcgacaga agtatcttta tctaccagaa gtccaaaatg atttcatttt
    46021 tgccgttatt gctgaagaat tggggtggtg gttttcgtta attattcttg gcctttatta
    46081 tttaattttt tatcgtggct atctagtggc acggctagca ccagatcgtt atggccagat
    46141 tttggcgatt ggtattgttg ggggcttact aattcaagtt cttattaact taggaggtat
    46201 tcttaacctt ttgccgatga ctggagtgac cttaccgctc attagttatg gcggtagttc
    46261 cctgatggcc gtcttgtttg gtttgggtat tttagctaat atctctagcc acactagttt
    46321 atcttctagc aaattaactt catgaagcta attaagagga gacgtaattt agttatttta
    46381 actggaggtg ggacagctgg ttcagtaacg ccactcttag ctatagctga agtcattggc
    46441 aataattata aagaaattag ttttttatgg ttcggcacca cccgaggccc agaacgtcaa
    46501 ttggcagagg aagctaaaat accatttgtg acagtagccg cgggcaagtt acgccgctat
    46561 tggtcttggc ataatattat tgacgttttt aaaataattg gtggattttt tcagggagaa
    46621 tatttgttga taaaatatcg acctaaagta gtagtaagcg ctggcagttt tgtcagtgta
    46681 ccaatagctt gggctgctaa attattgggt atccccctcg taattgaaca gttggattac
    46741 cggccaggat tagctaataa attaatggct aaagcggcgg actgtattct agtaaccttt
    46801 gctaagtcgc ttcaagatta tggctcaaaa gctatctggt taggcgcgcc ggttagttct
    46861 cagttaaata atactgagcc tgatgattat tcattaccct ggaaaattag tcgaaaaagg
    46921 ccgatagtat taattatggg cggtgggact ggagcgctag ccctaaatga agcagtttat
    46981 agagagatag ggaacttaac taagttagct gatataattc atataaccgg ttggggtaag
    47041 gggcaagaag ttaaacaggg tggttatttt tcggctgaat ttttaaacca tcggcagatg
    47101 atagtagctt ataaaatagc taatattgtg gtagcccggg cgggattagg gacaatgatt
    47161 gaattagcag cattagcaaa gcctactatt ttagtacctc ttcccaattc tcatcagcta
    47221 gataatgcca gggtagcagc tcaagctggt gcagtaatta tgttggaaga aaaagactta
    47281 gttgattatg ggttaacaga gatagtaagg ggtttgttat ttgatactca gaaacagact
    47341 aatttagtaa gtaattggcc caaggttatt aaaattgctt ccgaggcaga aattttaaaa
    47401 attttttccc gttatcttta attatggatt ggtctttggt taaaaaaatt tattttattg
    47461 gtattaaggg ggtcggcatg actatgttgg ctcaattttt acatcgtcgc ggttatgagg
    47521 tggttggtag cgacatcgct gatactttta tgaccgatca agtgttggtt aaagagggca
    47581 ttcgtatttt aaggggtttt ggcgataatg aatatcttcg tgccgctgat ttggttattt
    47641 attctacggc ctataatcaa gaaaatccgg aagtagcctg ggctctagcc aatcggccga
    47701 ctttaaccta cgccgaggcc ctaggagaag tatttaatca acattatgga attgctgtcg
    47761 ctggctcgca tggtaagacg accgtgtcgg cctggctggg ttttacttta tggcagagtg
    47821 gtttatcccc taatgtttta gtggggtcca atgttccgca atttgggggt tctgctctct
    47881 taggtaaatc cagcctgatg gtaatagaag ctgatgaata tcaaaataaa ctatattatt
    47941 tacaacctaa aatggtgctt ttaaataata tcgattatga ccatattgat ttttatccaa
    48001 cggttcaaga ctatgagcaa gcctttattg actttattgg taaagttccg accgatggtt
    48061 atttgattgc taattatgat gatcctacag ttcggcgtct ggcccccatt tattgtcggg
    48121 gtcagttagt aagctacgcc ttaaattatg ctgctgatta cgtagcttat gatttacaac
    48181 tagttaatgg gcggcagtat tttaaggtta aattgggagt tgacttagaa gaattagggg
    48241 aggtgcataa aaaaattgat aaattaatgg ttgaaaacca tggagattta ggagatttta
    48301 gtacagcttt gtggggtcgg cataatatta gtaatagttt ggcagtttta gcggccagcg
    48361 tagaattagg tgtaccgctt tataaaatta gagaaggaat agctgaattt ttagggacaa
    48421 gtcgccggtt agagatattg ggcaattttc aaggggctat tcttattgat gattatgcgc
    48481 atcatcctac agaaattaag gctactttag aggccttacg ggagcgttat ccgggggcca
    48541 aaataagagt tttttttcat ccacatacct ttagtcggac agctgctttt ttgaatgatt
    48601 ttgctcttag ttttatgatg gctgatgaag tgggcatttt acggatttat agttccgctc
    48661 gagagcacaa tggtgatatt tccggtcagg atttagctca aaagattttt tttcagcggc
    48721 aagcggctgg ttttaaaaac caggtaccat atttttcgac catggaagag cttactgaat
    48781 ttttaagaca gactgctggc aaggatcagg taatattgtt aatgggggcg ggtgatattt
    48841 ttagagtagg ccaagcgctt cttactcaaa caaaataata tgaagaaacc tttcaatatt
    48901 cagaataata ttcctttagc gccatatacg acctataaaa ttggaggacc ggctcaattc
    48961 ttcgtacgag tttcttcgct tgaagaatta tctctagcaa tacgatgggc taaggcggag
    49021 gggctttctc tgagattttt agggggtggt agtaatttac ttattagcga tcgtggggtc
    49081 aatggtttgg ttattacttt ggcggctcat gaaccaaatt ttttagaatc tggagtgaag
    49141 gttggggctg gttatggtct tagtaagtta gttaatgata gtgtgaagca aggacttcag
    49201 gggctagagt gggcggcagg tattccagga caagtaggag gagcagtgcg gggtaatgct
    49261 ggcgcctttg gtggctggat agctaacaac attactgctg ttgaagttta taacactcaa
    49321 gctgagagat tggaggtttt taagaaagag gattgccatt ttgactatcg tgacagtatt
    49381 tttaaaagac ggccggaatt attaatttgg tcagctactt tttcgttaac tttagcagac
    49441 cgggaatcat tagaaaaaat agccaaagag catcgtcatt atcggcgaga acatcatcct
    49501 actttgccta gtgctggttg tgtatttaag aatgtgccag taaatgaagt aatgggccgg
    49561 gcgccagagc tatggtcggc cgctcaattg gctcgggcag taagtggcca agaagtggct
    49621 gctggcttta ttattgccca ggctggcctt gctggctatg cggtggggcc agtacaagta
    49681 agcactcagc actctaattt tttagttaat cgcggtggag caaccgctca agaggtccat
    49741 cagttaatta gttatattca ggagactatt aagcgagtct atcatctaga acttgtgccc
    49801 gagttggcat tttgggagta atagggggtc ttttttattg attttttaag cgcatttgct
    49861 atactcaaaa taggttattg accactttta ttattgtcta aattgtttac taatgtttat
    49921 taaacgtatt gccattgatt tgggtactac ctataccttg gttcatttgc ctaaaaaggg
    49981 aattgtagtt aatgagccta gtgtagtggc agtatcaatg acggacaaaa aaattttagc
    50041 agtaggtaat gaagctaaag atatgctggg ccgcaccccg gatactatta tggccataag
    50101 accattaaaa gatggggtga ttgctgatta tcatactacc gagataatgc ttcgttattt
    50161 tattaataag gctttgggtg gtattcattt atttaggccc gaggttatgg tggctgtgcc
    50221 agctagcatt acttctactg aacggcgggc ggtgattgat gctactctgg ctgccggagc
    50281 tcgagcagct tatttaatta aagagccagt tgctgcggct attggggcta atattcctgt
    50341 agggtcagct tcgggccata tgattattga tgtcggcggt gggacagcag aaatggcagt
    50401 aatttcttta ggtggtgttg tgactgctac ctcagtacga gtaggcggta ataaaattga
    50461 ttcatctatt attgagtatg tccgtcgtaa atataatcta gctattggcg aaaggacagc
    50521 tgaagagatt aaaattggag ttggcagtgc tttatattta acagagaagt tattcatgaa
    50581 aattaaaggg cgagatattt tgactggatt acctaaaatt attactgttt cttccgatga
    50641 tgttactgag gctattcagc gcgaacttga ggctattgtt ggagcagcca aaaatatttt
    50701 acgagagacc ccacccgaat tagccgctga tattatggaa aaaggagcag ttttaagtgg
    50761 gggtactgct ttattaagaa atttggatca attaatatct aggaccactg atgtgccagt
    50821 aattattgct gaagatccgt gggcttcagt agtcaagggt acgggtatag ctttagaaaa
    50881 tctagatagt tataagcggt ccatttttgc taccaagtaa tatgattatt ggtattgatg
    50941 cttcacgagc caatcgagct cataaaactg gagtggagtg gtatgcttac catattatta
    51001 aaaatttggt agagattgat cctgatgatg attatatttt atatagtgat aaacccttgg
    51061 agggtgattt actacaatta tgtaaggtgc attctaattg taaacaaacg gtgctacatt
    51121 ggccattgag atatttttgg accttgggcc ggttaagctg ggaaatgatt gtaagaccgc
    51181 ctgatgtttt atttgtcccg gcccatactc tacctttatt ttctagagct aagatggtta
    51241 atactatcca cgatgtcgct tttatggttt atcctcaatt ttatagtacc ttagacagaa
    51301 agtatctgca gtggtctact aggtttgcat tatcgcgagc cgaacagatt attactgttt
    51361 ctaattttac taaacaagag ataatgcgtt attttttaat ggcccaggaa gataaaattt
    51421 ctgtggttta tcatggttat gatcgggagt tataccatcc tattactcac caatctgttg
    51481 ataatccagt attagattat taccatttgg aaaggccata ttttatatat gttggccggc
    51541 tggaaagtaa gaaaaatatt attggtcttc ttaatgcttt tcgtaatttt tgccaagaaa
    51601 ggcctcagga taaaactaag ttagtattgg tgggaaagcg aagccgggat tatgctccca
    51661 ttaataattt attggctgat cctattatta aagagcgagc taaggaatta gggtgggtac
    51721 ctgaaaaaga tctgccggct ttattacgac agtcattggc ttttatttat ccttcatggt
    51781 atgagggctt tggcctacca attattcagg ctatggcttg tggcacacca gttatagctg
    51841 ctcgggttgg ttcattacca gaaattggtt ctcaggttgc tctttatttc gatccagcgg
    51901 accaaggaga cctgttagaa aagatgaaac agttaaccga tgattcagct ttacgccatc
    51961 aattatctca agctggactc aagcgggcgg cgcaatttag ttggtctcag gcggcagccc
    52021 aaactttaga gatattaaag aaaagtcatg gctagattta gattaataca tattagaaag
    52081 atattggggg ggttaattat tttagtgctg gctatcttta ttggtagtaa agttaaagct
    52141 gatgaattat ataagatagc cttgctgccc tcagatagtt attataataa tcaatggtat
    52201 ctccctaaaa ttaaagcccc tattgcctgg gaggaacaaa gagaaagccc ggatattgtt
    52261 attgctatca ttgattcggg ggtccagatt gatcatcctg accttaaaga taatatttgg
    52321 attaataatc gtgagatacc taataaccaa aaagatgacg acggtaatgg ttttattgat
    52381 gatgttaacg gttgggattt tgttaataat gttccagatc ctaatcctaa atttcaacct
    52441 ggctttaccg aagaaggtgt gtttcatgga acaattattg ctggtgttgc tgccgctatg
    52501 ggtaataatg cggcaggagt agcgggggtc acttggcggg ctcaaattat gccccttaag
    52561 gctttagatg atcgaggatt aggagatact tttagaatta ttcaggccat tgattatgcc
    52621 attaataatg gagctgacat tattaattta agttttgttg gctataatta tagtcagagt
    52681 ttagctgagg ccatagaaag agcatataaa gctggagtaa ttgttgtagc cgcagccggc
    52741 aatgaagaat cagggggcta tggccattct ttggataaat tgccgatgta tccagcttgt
    52801 cttgatggag gtaagaatat ggttattggt gtagcctcta ccgatgcctt agatcaaaag
    52861 actaaatttt ctagctatgg tgctcgttgc gtagatatcg cggcgccagg agtgagtatt
    52921 tttagtactg tggtttatag ccccaatcga caaattaatg gtcggttgct taataaatat
    52981 tatgatggtt attggtcagg gacctcagtg tcagtggcaa tggttagtgg tgcattagct
    53041 ttaattatgg agaaaaatcc tagtttaaaa cgagatcaag tagtccaagt tttattaaat
    53101 aatgctgacg atataacccg gctgaatatg gattatttgg gtcaattagg gagagggcgg
    53161 cttaatattg cctccgctat taatgatgcc tctttacaat tatctaataa agtgagtcgt
    53221 tttttggtgg caccagcaag ttctagtagc cagccagtta aaattcttga ttctgataat
    53281 cacccggtgg gagaatttta tgcttttggg actaattttc cttatggttt gagagtagcg
    53341 agtggtgatc tggatggtga tggtgcacca gaaatagtgg tagtgcccga tgaaaaaggt
    53401 caatcagcgg ttaagatctt taattataaa ggtaaattag tcgggcagtt tattgccttt
    53461 agtaaggatt tttcggctgg ccttagggtg gctgttggca atgtagacag tgatcgagcc
    53521 ggggaaatta ttgttggcac tggttatgga gctaccaatg aagttagagt ttttacggca
    53581 caaggagtat taaaatataa gtttaggcct tatgatgcta attttaaggg tggggttagt
    53641 gtagccaccg gcgatattaa tggcgatgga ataatggaaa ttattactgg tgctggtaag
    53701 ggtggtggac cgcatgtacg gatttttact ggtaagggac aagtgcttgg acaattcatg
    53761 gcctatgaat cgaattttca tggaggcatt gaagtttttg ctggcaattt tgactatgga
    53821 gccaaagcta atcgtgatga aattgtagta tctccccgcg ctggtcgttt acctgaggtg
    53881 cgaatttttg atggtcgtgc taaaatgatt tctagttttc ttgcctataa taataatttt
    53941 cagaaaggct tggctgtggc tttaagtgat attgatagag atgggctaat ggagattatg
    54001 acggctgcgg gtccaggtgg tggaccgcat gtccgggttt ttgaagtaga tggtgagctc
    54061 cgtaagtcgt ggtatgtgta taatccgtta tccagcagtg gggttaatat tgctcctctt
    54121 aatttaagta gacaatttat taattgataa ttatatggcc gtgcaaaaaa tttttgataa
    54181 aaaaaatgtt ttagttactg gtggtgctgg ttttattggt tcgcatttat gtgatgaatt
    54241 gattaaaact aacaaagtta tctgcattga caatttttta ggtggaagtg aacgcaacat
    54301 tgaccatctt ttagctaatc ctgactttat ttttattcgg catgatatga gtgagcctat
    54361 agatctattg actataccag agctaaatcg ttttaaggtt aaatttcaag gcattcagga
    54421 gatatataat ttagcctgtc caatgtcgcc cattgatttt atgcagaatc gaatagctag
    54481 tttattagcc aattcagtgg cggtgattaa tgctttagaa atggctagac aatatcaagc
    54541 gaagtttatg catttttctt cttcagtggt atatggcact cgtgaagtac atggtggcaa
    54601 taaagtagcg gaaacagatt tgggaatagt ggatcaaatt tccgagcgag cttgctatga
    54661 tgaaggtaag cgttttgcgg aaactatagt tagtacttat cgagattatt atggattaaa
    54721 tgctaagatg gtacggttat ttcgaattta tggtccacga atgcctatta atcaggggca
    54781 tatgattcct gattttgtag tgaatgcttt agataataag gatttgatta ttaatggtga
    54841 tgaaaatttc acttcatctt tatgttacgt ctctgatgcg attgatggag tgttaaagct
    54901 aatgcagagt gaattttctg gcccagttaa tattggatca gaagttgatg ttaaacttaa
    54961 agaggtggca gagaaaatta ttcaaaaaac tggttcctcc tctaaaattg tctatgcgga
    55021 tagactgtta tttatgacag aacttaattt accagatact actttagcac gggagcagct
    55081 tggctggatg ccggtagtgg gcttagatca tgggctagat aagaccatag aagatttaag
    55141 gtcgaggaag gctttaaaag aattaccaac taattggtaa attatttatg aagatagttt
    55201 gtgtcatacc agcttggaat gaggctaaaa ctattgtcaa tgttattcag gatgttaaaa
    55261 aacatgttga cgaagtgatt gtggttgatg atggatcaga agataaaacc gcaaatttag
    55321 cccactcggc gggggctact gtcttagttc atcgtcttaa tcggggacag ggagcagctt
    55381 taagaactgg gacaatttat gccctaaatc atggtgctga tattattgtt cattttgatg
    55441 ctgatggcca atttactgcc caggatattc ctaaagtcat tgcccctata gtgcaaggag
    55501 aagctgacat agtttttgga tctagatttt taggggcaga acataatatg ccgccattta
    55561 agaaaagagt tgtattacca ttggcccgtt tatttaattt agtgtttttg ggggtacgag
    55621 tacgtgatcc acaaaatggt tttcgggctt tatctgccaa agcagcgcgt caattaaact
    55681 ggcagcaaga tagaatggcg cattgttctg aaatcttatg gcttactcgt aaagctaagc
    55741 tgaggtatca agaagtagga gtaaaggtta agtataatga ttttggacag aaaatgtccg
    55801 gcggctggaa gattatcggt gatttatttt tagggcgttt tcttaaataa ttttattata
    55861 tgttacaaaa agttctagct ttaattatta ttgccttttt tctggctaag ttattatggc
    55921 aacagcatga gcgaaaatta tcgtggacgg aatttttttt ctggttatta ttttggttgc
    55981 tctcggctgt agccattttg atgctagatg atattgatag attggtagct aaattaggtt
    56041 tttcgggtaa tggaattgag gttcttttat atgttggttt tgctcttttg ctgtatctta
    56101 tgtttcgttt acgtttaaaa ttagaaaaga tagagcatca gttaactacc ttagtgagtg
    56161 ctatggctca acaaaaaccg ctagagtcaa gtcaggataa taaggaagat aaataataat
    56221 atccatgaag ataattcatt tggtttgtac ctttccgcct tatcaagggg ggatgggcaa
    56281 tagtgttaaa gacatggtgg agagattagc tttaactgat ccttcttgtg atattactgt
    56341 agtttgtcct aggtatggtt taagagaagt ggcgccagtt agcactttat attcggtgag
    56401 gcgctgttca agctggctgc gatgtggtaa tgcggcagtg attaaggggc taaaaaaaat
    56461 atggcagtca gcggacatag ttcatctaca ttatcccttt tatgggactg atgctctagt
    56521 atggtatttt aaaaaaaggc atcctcacac taaattagtt attcattacc atatggatcc
    56581 gcaggctgac ggctggaaag gcaggatatt taaaatttgg caaaaattat ttttaaagca
    56641 attacttacc caagccgaag ctattacttg cgcctctcta gattattggc gtcactcatc
    56701 agctggccgt attgatggtt taagtgacaa attgtttatt atccctttta cggtagatac
    56761 taatcgtttt cagccacgtt ctaagaatca aactttacta gaaacatatg gtcttaagag
    56821 tgaagtgcct atcattttaa tggttggagg gctagatagg gctcattatt ttaagggagt
    56881 accagttttg cttcaggccc tagctcagtt gaaagttaag cttggtaaaa attattgcca
    56941 aggaattatt gtgggagaag gcgatttgcg aaataagtat aataacttag cccaaacatt
    57001 aggtttaagt catgatgttc tttttattgg tcaagttagc caggacaact tgccatttat
    57061 ttataatcta gctgatttat ttgttttacc ttccattaat agttgtgagg cttttggact
    57121 agtgctttta gaagcaatgg ctagcggctt accatgtttg gcctctaatt tgccaggggt
    57181 ccgggaagtt atcgatccgc cccgtaatgg tcttctcgta gagcctaata atgcagatga
    57241 tttagctaat aaaattagtc agttattggc agacgactta aaattaaaag agatggctca
    57301 agcaagtcga gatagagctg taaagtattt tgctagctca acccccagcc ccttggcgcc
    57361 aatttatcat cagctaacag aataatcacg atgaaataat tatgaaaatt ttattagcta
    57421 ataatgtttt ttccccttat gagcggggag gagctgaaga ggtagtgtgc tacctggctg
    57481 agtcttggtc tgaaataggt catgaaatta tggtggtgtc tctagctccc agggggcata
    57541 aaaaaataag gaagcaagac aattcatatc aaatagttta ttggcccagt aattattatc
    57601 gtttatctaa tttatttaaa tggcaacggt ttgtatggca cttgcccgat tggctaggct
    57661 taaggcatca ttggaaatgg cgaaaagttt taaaaaaatt tcagccagac ttggtagtgc
    57721 taaataattt aactggatta ggttttgagg ttcatcatct ttgccagcgc ttaggtattc
    57781 ctagtgttca ggtaatccat gatgtccaat atcttcaacc aagcggtttg ttacttttag
    57841 gggaagaaaa gttgttagct aataattggc taatgaggtg ttacagaaat ttaacggcct
    57901 actggctaaa accggctaaa gtattaatta gcccctcata ctggctcaag gatttttatt
    57961 gtcattataa ttggctagga gataactgtt ggcttaagtt agctaatcct ttgaagataa
    58021 cactatcttc aagctatcga ttaccagacc aactgcgtca agcaatattt gttggccagc
    58081 ttactccggc caaaggcatt ggttggctag cgggacaatg gtcggatttt aacaaattat
    58141 tattagcagc cgggcttcct gaagccaatt taacgattat tggtgatggg ccgctttttt
    58201 cctctttaaa ccgtgaggcc agtcttgatg aacatttacg tctaatgggc cgtttagccc
    58261 atgaagcagt gcttgaccaa ttaaaaatag ctgatgtttt aattatgcct tccttttgtt
    58321 atgaaaattg gccaactatt cttttggaag ctgcttctat gggctgttta gctttagtgg
    58381 taagtcatgg gggagcccaa gaattagctg aagctttggg ttatttaact tttgaagccg
    58441 gtaacttaaa tagccttttt aatgcctggt cacaattatc tttagctgca gctaaattaa
    58501 cctattggcc agcgggtgag ggtattaaga tgataacgcc tagggattat cttactgaga
    58561 ttttaaaaaa tattaactaa agattgttat acacaaagat aagaattgct ccattggcta
    58621 tttttattat ctcatcagct tcagtagcgg cttctttggc tattttttca aagccatacc
    58681 aataattatt aagtacaaaa taagcctgat gcactccagc taatttagca gcttcagcta
    58741 ttgtttggcg tgacggatag tcattgacca tttttagata ataagtataa agaatacccg
    58801 atgtaggtat agggtaatag aaaagattat tctgaagata gcgttggtgc ttcccggggt
    58861 aaaaaccata ttgcgccagg gcggcagcac taacttgttg attggctagt actatatagt
    58921 tatcattagc tcgttgttca attatttgta ctgcttggaa gtcagcttga ctagtagata
    58981 agccttgggc gtgagaataa cggtcgtcac gggggtaggt gagatagagg ctaaaggtta
    59041 taagagtagt aagcgcaata ataattggcc agatcaattt tttataatgg ctatttagcc
    59101 atttgtgagc taataagcta aaagtcatca aagcagctgg tagattaata attatagcta
    59161 gccataggaa tctagttaga taattatacc gttcataatc aattataaaa ttaaaaggaa
    59221 caaaacttgt taagattata gctatcatta gagataaatt aagcaattta agaattgaag
    59281 cccaggtagt tttatttttt cttaataaat gccaaccagt taggataagc cccatagcga
    59341 ctagtggttg ccaagcttgc catccataaa ttaaattaag gagccaatgt tcctgattgg
    59401 gccataaatg agaaaattgg aagaaattag ttagttcttt aagtagatta ttaatcggtg
    59461 gttttgccaa ttgccacttt tttgtttgct gccaattggt tagccacaag gaaagtggaa
    59521 ggactagagc ggtaaaggtt gatgccagcc acttaagact tgtttgccaa cgtcgttgaa
    59581 cataagataa actacttagc agagcaacgg ttatggctgg caaaccggca aggggatggc
    59641 agactaaact agctaaagct gatgaccaaa ttaggggaga ggatttattg ggccaagtag
    59701 cagcaataat tgtaataata agaaaaaagt aggcacaatt ttgtgggaca gtaacgatga
    59761 agattggcca ggtaaatatt ggccatagcc agacagctag acggccactt tggtcagagt
    59821 ggtgattttg gcgaaaccac tccataataa accaggggag aaagcaggcg attaagagag
    59881 gtaaaagcca gcgttcaatg gagctaatag ctagaccagt taaatgatga atagtaataa
    59941 ctagtgaata ttggcctaaa taatatgggg ttttagggtt aatttgacct tgagccacaa
    60001 tggttttttc agcggcctgg tggataaagg ggtcaaagcc ataagctgaa taatataggg
    60061 agctagctat tccataaatt aaaagccacc aggcgataat ggttaaggtg gatatttttt
    60121 tattagcttt aatagttatt aggccagcag cagcaataag ccagcagatt agccagaagt
    60181 accaattgac ctgagtccac ggagatagta aggggagggt agtcttttgt tttaataggt
    60241 agatgaaagt tattattatg gctatgagta taaaccaact tattatagaa ctttttttct
    60301 gcgggggttt ttcggttttg ctttggggtt gctttcggta tataataaat aaagttagga
    60361 ctgttaacat taaagtaatt atttctccta accaattcaa accatatagc cagtaaacaa
    60421 ttgttcccca aatactgatt aaattaataa tcaataatat tgtcggtggt tgttttaaat
    60481 atgttaatat tttcatgatg aaattactaa aaattaataa atcttctgtc gctattagtg
    60541 tttttttaat tattattggg ggtttatttt ttataggcac cagcgcttat tcatattgta
    60601 tccagcgacc tggttttgtc aaatggtctt cacctgacga aacggcaaat tattatttta
    60661 gtaaattatg ggcggaaact ggtagcctta tagcccaaag cccaattgac gtccccgagg
    60721 gggttcttgt tcgaccgcgc agttttaagg tggttgacaa tactttacgc ccagtaagtt
    60781 ttgtaggttt gcctttgctt tatggttttt tggcacggtt gtcttctgtt gatattttac
    60841 cgtatttaac tccattcttt gcagtcttgg gactgttggc cttttttggt atagtgcgaa
    60901 gactttttaa tttgcccacg gcgattattg ggactacttt agccgcattc tttccagttt
    60961 attggtattt tactgccaag agcatgttcc ataatgtttt atttgcagcc tgtggtctta
    61021 tagcttgtta ttgcggtctg cgggccctag atagactgcg attagcgcgt catcagtcag
    61081 ttagttactt tcccgccttg atatggttag ctgtttctgg tctagctttc ggctgggcta
    61141 tagctacgcg gacctcagag ttattatggt taatacccat agcactaatt ttagtgatag
    61201 cttattttaa agaaattggt tggtaccgtt ggctctggtg ggctagttgg tcctggattg
    61261 gaatgttgcc ggccgtttat tggaatatta ttttgtacga ccattggtat ggcggcggtt
    61321 atccgactat gagccaatct attgcaacca ttagtcaaac cagtttatct ctatttaatt
    61381 ttcataattt agccgataaa tttactgttt tgtggcagac tattttccat tttggttttt
    61441 ggcccaagca gtcgctgacg atgtttcagc attatgtaat tgaaatgttc ccttggttaa
    61501 tgcctttggc cttgattggt attttagtct ttatttttca tcgtcggcag tggaaaaagg
    61561 ataaatggtt atatttattt ttgtggatat tagcctctgt atttttagtg ctatattatg
    61621 gcagctggac atttaatgat aatcctgatg ctagccattt tactattggt aattcttata
    61681 cccgttattg gttgcccttt tatctagggg ctttgccttg ggcggcggag ggattatggc
    61741 aactggctaa ttttttattt cgacgccgtt tagttaagcc aattgttact ttagcggtta
    61801 tagggataag ctgttggtcg ctgcatttta ctgccgttgg ctctgaggaa ggtttaaaat
    61861 ttaccgctga gcggcaagta gcagattatc atttatggtc tgaagttcta tctaggactg
    61921 aagatgatag cattattatt acgctttacc aagataaact attttttcct gaacgtcagg
    61981 ttcttgtagg tctttttgat aataatgtta tgaatcgcta ttatggcaac ttacttaaaa
    62041 aatggccagt ttattatttc agctttcgat tttcagatga cgctctagaa tatctcaaca
    62101 atcgtcgctt gccgcctttt ggctataaat tagagaaaat ttgggactat aatgaatttg
    62161 ctttgtatcg tttacagcct attccggagc tacctttaaa tgttagcccg caacaatgat
    62221 tttatgaagc agagagcaga gaactataat attggcattg atgcccgttt atacggcccc
    62281 ctgggtcgag gcctggggcg ttatattgaa gaagtagtta agcgagtagt tgctcttgat
    62341 ccaacgaatc aatatattct ttttttaagc ccgcgaagtt ttaatgattg tcagattgaa
    62401 caagctaatg tccaaaaggt tattatgcct caacgttggt atagtttggc ggagcagtta
    62461 gcctggccac gattaattta tcattaccat ctggacatgc tgcacgtacc acattttaat
    62521 gcgccgttga tttgtccttg tcctttagtg ataactattc atgacttaat attaactcag
    62581 ttccctagtc gtcgagctag tactttatcg ccagtagttt actggtttaa aaatttagcc
    62641 taccatttag ttattgctcg agctgtgaag aaagctgaaa caattattac catttcagag
    62701 tttacaaaaa aggatattat ccaacaatta gccgtaccgc ctgacaagat tgaggttatt
    62761 tataatggtc tgactactat ttcatcaggc tcatcctatg actttaaggc tgacagagat
    62821 gtacttttgc gctataatat caaagcacct tattttcttt atgtgggtag tgcttatccc
    62881 cataaaaatt tagagtggtt agttcaattg tttataaggt ggtcgaaaaa gcatcctggt
    62941 taccagttag tgctggtggg tgagcacgat tatttttatg agcgtcttat aaggtttatt
    63001 gctgatttac ctgagaaaga aagcaggggg gagattattt gccccggatt tgtgcccgac
    63061 gaagcactag ccgcactata tcgtcaagcg gctttgtatg tttttccttc acgttatgaa
    63121 ggttttggat tgccgccatt ggaagcaatg agttatggat gtccagttct atctagtgac
    63181 agttcgtgct taccggaaat tttaggatcg gcggttagtt attttaagga taatgatatg
    63241 gactcagcgc taagggcaat ggagaaaata ttgcataata aaaattatcg ggaacggcta
    63301 attgctttgg ggttggaaca gataaagaaa tatagctggg ataattgtgc ccaagaaact
    63361 ttaaagattt ataaaaaagt tttataccat taatctttat aaatgagaca gacaagtaaa
    63421 caatataata atgatgatat tccgcgatct gattttcttc tagatttgcg gcaacaaatt
    63481 attgatgagc ccttagtaaa aaagcacctt agcaagagaa agaagcggca atttcattgg
    63541 ccaaagattt ctaaatttac tttatggggt aggggagggg taagggtgaa aattaaatgg
    63601 ccgaccatca aacgtccaag attatctgtt aaaattagtc ggcctgccag tttgcgtcct
    63661 ttgtggcatc taataattat ttttttagtt attgttattc ccctgaagtt aatagtctat
    63721 attgaagatt tgcggggaat gcgggtaaga gtggttaatg cttctagtga aggattggct
    63781 aatctggaga ccgcaggtca actattaatg aatgaagatt ttacttcagc tagtagtgcc
    63841 tttactcaag cctcggccag ttttgctcat gccaaagaga atatcttggg ggttcaatct
    63901 tggctttggc agctagccgc tcttgttccc agtgagcaga tgcacttagg cgctaaggct
    63961 ccatatatta ttgaggctgg taatttagcc agtaaactgg ggactgattt cactatctta
    64021 gctcagcaca ttgtagataa aattggtaat aatgacccgt tatctttaat gcgacaaatt
    64081 aatgtagata gtagtcaggc gttagcggat agtgtagctt tgaataatat tttaaataaa
    64141 attaaaccag ctagtttgcc gggaagttat cgcgagccgc tagaaaattg gcaggctaaa
    64201 ttgcagacta ttagtaattc attggagcag ctacaatttt ggtcagcggc tagtttagat
    64261 ttattaggag agaagatgga tcgtcgttat ttattagttt ttcaaaataa tgctgaagct
    64321 cgagctaccg gtggatttat tggcagttat gctttattag atgtaagtcg ggggcaaatt
    64381 aagaatttag aaatacctgg agggggtagt tatgatactg atggcaactt acgtcggttt
    64441 gtcaacccac cggccccttt aagattagtt agtacctatt ggaaattttg ggatgctaac
    64501 tggtggccgg attggccgac ttcggctcgc gagttagagt ggttcttgct agaaagtaac
    64561 gggccaagtg ttgatggggt tattgctttg acgcctgatg ttttagagac tttattaacc
    64621 attactggtc cgattgattt aactaaagat tacgggttag ttattacagc cgataatttc
    64681 cgtcccttag ttcaggatta tgttgagaac aaagtgagtg gtcccaagga accaaagaag
    64741 attattggtg atttggccgt ggctattttg gaaaagttgc cacagagctt aaatgaccaa
    64801 gcaagcattg ttaaattgct agagcaaatg agtacggagc tggccactaa acatatttta
    64861 ctttatttta ataatgaagc aattgagcag aggtgggagc ttacaggttt agctggtcag
    64921 ttagcaccgt taacgccagg tcaagattat cttcaggtag ttaatactaa tattgctggt
    64981 ggtaagactg atttaaaaat tagacaaacc attgaggaga gaattgaaat ccaagaagat
    65041 ggcagtgtca tcaatgatgt gactattatc cgtgaacata ctggtgagag gggggatggt
    65101 ctttacggcg tacgtaatgt agattggcta agactttatg tacctgaggg cagtgaatta
    65161 ctggccgctg atggattttc agcgcctgag agtcattttt ttaaaccgca agatgttacc
    65221 gctgaagata tgccggcaat taagttaggg gaggggtcag cgcaaattga gccatcaagt
    65281 ggtactttag tatataatga attagggcgg actgtatttg ctaattggtc gatggttgat
    65341 ccgggggaaa ctgtccgttt aaattttaga tatcggttgc catttaatgt cttagaagtt
    65401 gggggtaata ctgattggct ctctaaggtg aaagcttatt taggcttagg tcgtattggc
    65461 aagtaccaaa taattattga aaatcaggcc ggtcagtcgc cgacgacttg gaaagtgatg
    65521 tggagttggc cagctaagtg gagtttatta tggtcttcgg tagacagtat taaggctaac
    65581 acttggcaag ttaatgatat tgtaactaag caggcgatcg gtttactatt taaagtagat
    65641 aattaatttt attatgccca agaaaaatat tagcaaaaaa tcatctattg attattttga
    65701 acaaatgttt caagctggtc agccagtaat aaaaaaggtg gaacataaaa gtcataaaag
    65761 taaggaggaa ttaagagagc gggaagttga agatagtttg tcagaaattt atcaagatga
    65821 tgcggggagg aatattgatg tgcagaaaat ttctgttagg ccgcgacggt ggtggctaaa
    65881 aataattttg tttttacttt atagcgccat tattatcgcc attggttatt tgggttatcg
    65941 ttattatatt agttatcagg ctaaagatga tttatttaca gtagtggttg aagcggataa
    66001 aaatttaact gctggccgtg aatttgctta tactatttcc taccacaata aagggcggtt
    66061 agctctagaa gatgtagaga ttaaagctga gtggcctaag agttttatat ttagaggggc
    66121 tgacccagct accactagtc tcaatgtatg gcatattggc catgtacccg ctgaaggctc
    66181 gggacagata gtggtcaagg ggatactact taataaaatt ggagaaaata atcaggttca
    66241 ccttacttcc agttttcgtc ctgctaattt aagtagtgct tttgtaatta atactaccta
    66301 caatgttatt cttactgatt cagttttagc tgttggaata tcggctccag acgttttagc
    66361 tattggccgg gataatgaag tgattattag ctatcaggaa aaggaagaga gccttgtgga
    66421 taatgtgcaa ttaataatga atgatgtgga ttgggctgaa gtaaaattat tcgacggtga
    66481 tcaagagatt caatccactg gcaattttag gtggacccta ccaacaccga gcagtgaatt
    66541 aaaaagtctt aaattagtta ttaaacccag tgaacaggaa agcaggctag aaatattaag
    66601 tttcagatta gagaccaata taggcgagca aagttattta atagatagcc gtgattttga
    66661 ttgccaccta gttaatagcc gtcttaattt actcttaaag attaatgata gcaatgctga
    66721 tagtggggtt tttgccggca cacctttaaa ttaccggatt gactatgtaa atcaagggga
    66781 tacaacctta aaggatgtta aaattattgc ttctttagaa ggggcatggt tagattggtc
    66841 atcgttgaaa gatagtcagc aaggacaaat taagggacaa actattatct ggagtaagaa
    66901 ggaaattcct agtctagctc ttttaaagcc aggagctagt ggctcaattc atttttcaat
    66961 taatgttaaa gactggcaaa atggagataa aaatgattcg ggagagatta gaagttatgc
    67021 ttactatcaa acaggggata aagttattga gaaacctacc gatgaagaaa agagtaatac
    67081 tattattaat caacttaata gtgatttaag tttaactgaa tccgttgtct attttaatga
    67141 agacaatatt gcggtgggtt ctgggccagt acccatggtg gttggcgaga ctactaattt
    67201 aaaagtatat tggcgtatta gtaatagcct tcatgactta cgggatgttg aagtaatcgg
    67261 ggaattaccg gattatgttg attggggtgg caagaatcat tcttcggtcg gtgaattaag
    67321 ttatgatgcc gacagtagac aagtaaagtg gcatatagac cggctacctg ctaatagtca
    67381 ggaggttagt ggtgatttta gtattagtgt tacgccacgt cctgaccaaa agaatcaaat
    67441 tattgttgta ctgcctggtt ctactattac tgctattgat aatgttactc aggcgcggct
    67501 agaatttact actcaagcta agactagtcg tctggaagat gatactatag ttcaaaccga
    67561 cggtttagtt caataattac ttaataataa atattatgaa tcagtttatt cttaatcaaa
    67621 ttgctgattt tcaaaaagcc attgattttt ttataaaaga tattggtacc ttgcggacag
    67681 ggagagctca tcctggtcta ttagataata ttgttattca ggcctatggt gattattatc
    67741 ccatcaatgc tttggccaat attagtgtgc cagagtctcg ttctttggtc ataatacctt
    67801 gggacaagac agttttaaaa gatattgaga agggattaat agcggctaat ttgggcctag
    67861 gcattgttaa tgaaggcgat aaaattcgtt tgactattcc tccccttaac gaagagaatc
    67921 gacgtgattt agttaaaaga ttaaatgata aaatggagga ggccaggatt gtcttgcggc
    67981 aagcccggga ggcagtcaaa aataaaattg aagcggcaat ggtggctaag gaaattagtg
    68041 aagatgacaa atttcggttc attaaagaat tagatgaatt tttagcccag aaaaatgaag
    68101 agttaaaagc cttacgagat aagaaagaag aggagataat gacaatatga cagtagtaat
    68161 ttttttaggg attctcggaa tattagtgtt agttcatgag ctagggcatt ttttggctgc
    68221 cagagctatt ggggtaggag tagaagaatt tggattaggt tttccgccta gattagtagg
    68281 gtggtttaaa aatcgacatg gtcattggaa attattaaaa aagaagcatc accatccgcg
    68341 acatactatt ttttctatca accttatccc cctaggtgga tttgtgaaat tgcggggtga
    68401 agatggggtg tctcggccaa gagaccatac tagttttgct aataagacag taggtgagcg
    68461 ggctataatt attatctctg gggtgctaat gaatgttgtt ttgccaatga taattttttc
    68521 tctcggtttt atgattggtt ggcctcaggc tacggaggat ttagatcccg gggctagagt
    68581 gaaaaatccg gaattaatga ttgctgaggt agtaccaaac tcaccggcta gccgggcagg
    68641 cctagagcca ggtgatgcca ttattgaaat taatggtcag cccgccagga gtgctgctga
    68701 attgcgtcag attaatgctg cggcagcagg tcaagaggta aactataaaa taagacgttc
    68761 ttcagaaatt ttagagttgt ctattgccgc ggagcagttg gctagcaaca ataatgacta
    68821 tagtttaggg gtagccctag ttgagagtgg attagtgtca tatgcttggc ctagggctat
    68881 ttgggaaggt attaaattaa ctgccttaag tcttaaagat attgctgttg gtttttggca
    68941 gctttttact ggactattta agggtcaaga tatatctggt gacgttgctg gtccggtagg
    69001 catagctgtt ttggttggcc agattagcca tttaggctgg atttatcttt tgcagtttat
    69061 tgccctactg tctcttaatt tagcctttat taatattttg ccttttccag cactagacgg
    69121 ggggcggcta ttttttattg ggcttgaagc tattacacgt catccagcta aggctgagct
    69181 ggaagcgcta attcatagta ttgggtttgt tttacttttg gccctagtcg ctttgattac
    69241 tattaaagat attgccaaac tattttaata cttaaagttt taataagtta acatgcggtt
    69301 ttctcgaagt ttcaaattac tgattaattt tattagatta actttggcgg gggccatagt
    69361 gttagccatt attttgcata gttgggctaa tttattaatt attattttag ctgtcatttt
    69421 aacatttttg ccagaatggg tagaaaaaaa atataatatt tatataccct taggttttga
    69481 gtttgctgtt gttttactaa tctatgctag tctattttta ggagaggttg gtcgttttta
    69541 tgaacgtttc tggtggtggg atgttttttt acattccttt tcttccattg ctttttcttg
    69601 tttaagcttt attgctttat tctttttatt taaagcagat aaaattaaag gccgtcctat
    69661 ttggattgct attttttcat tctgtttttc agtcgccatt tctactctct gggagatttt
    69721 tgaatttttt atggaccaaa cttttggttt taatatgcaa aagagtggcc ttatggatac
    69781 tatgggagat ttaattagcg ctagcggcag tgctttgttg gtggcaacta ttggctattt
    69841 ttatttaaag agtgatcaaa aaactatttt gcatcgctgg ctggattgga taattaaaaa
    69901 aatatttgca ggcagataaa aaaatatttt ttgtaactgc ttccgctatc gctgaatttt
    69961 gtgccaatga agttggcgta tcgcctgaaa atctaaaatt cctcgtggga ttatttagga
    70021 tattagttat atactattct ttgtaaatat tttaatttaa ttttaaactc ggctcaacaa
    70081 tttttctaat ctcttcacag ccatgtactc tgccagagcc atcatcaaag cctgagccgc
    70141 aagaactaac aaaaaggagc cagtttttat taactaatgt aggataggca tcaccaccag
    70201 ccgaacaaat atcaaaatct tttttaaatt gatcaagtga tgtatatcct ggtttgtttg
    70261 gcaagacagt aactttaaaa atgccatcgt cttgtgaatt ttctttatat ttaaaagtat
    70321 agacaatttt aggggtgtta ttaaatttag aaactagttt atcaaaataa tcagaatcat
    70381 tattaactcc gcattctaag gccgatgatt gtagctgact ggacgtaaaa tcaaattgtg
    70441 tttcctcttt tgaaacagat atgaggctgt ccacaattga ataagacaat ggatcatttt
    70501 tttctatcgt ttcagttaaa atattatcta tatttttata actcataatt agtattggaa
    70561 catctgtgtt ttcaaattta tatattgtaa atttacaagc atcgttatta caatccttgt
    70621 tttgtatttc ttttttatca acaaccaagt tatataaata caactttgaa ctagaagcat
    70681 tattttgatt atataaaccc atcaaatcat tattaagcaa caaggctttg ttaata
//
LOCUS       DAOOIS010000003        44792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_935_length_44792_cov_35.932584, whole genome shotgun sequence.
ACCESSION   DAOOIS010000003 DAOOIS010000000
VERSION     DAOOIS010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 44792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44792
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_935_length_44792_cov_35.932584"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..553
                     /locus_tag="PK960_00820"
     CDS             71..553
                     /locus_tag="PK960_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07752.1"
                     /translation="MIHKLKKILSQPYFWLIIGIILVIVILGLIVGPTILEKVEWHKW
                     QAPFFNDARTSLGGESPEEAYQKFWQAMQANERDTALAYVIGVRRPYFQRAWEDPALW
                     ALDQSLPETTSLVFIGDCLPDAIACKKRAVLSYSTATTTGRIELYQNLAGRWQIGDIY
                     "
     gene            558..5207
                     /locus_tag="PK960_00825"
     CDS             558..5207
                     /locus_tag="PK960_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPD07753.1"
                     /translation="MLRRGKSHLRLQYFLLFLLLGFVGPRAVLAYDHQITHPRFTSWS
                     VQIYNLAFRPVITNQELGWLAAGAAAEDQPVLRTMNHFWWPQTDRPLSAGSYHLMSLE
                     TAPDWGLAPGAQANLIYGPSFSWYQAIEAYRRGDYETAFVNLGHVLHLLQDVSVPAHS
                     RNDPHVLGDALENWVFQHHEELAVLPALVRPRCQDYRDCFRQMASAVNVNFLSQDTIN
                     SPAQAGDWPDKPVIKDWPSESGYVIYRGYRLVYYNEPRRRFILDDIVLSDYWSQIAPL
                     TVGYGAELLRLFFTAVADPQVVPETTSWFTRWRSTIEAGSGAPVEIIDNPAKITDNLT
                     SSPAIVSSSLISPGSTITSSVAIDSKSNSTSTPTGVKTNQASTSQALIAQGLNVQLKR
                     VIDGDTIELTTGEKVRYLGVDAPELASTSSRASECLAEEAKSRNQVLLTRGPLTLIAD
                     SGGDKDSYGRLLRYVYAGDIFVNKTLAAEGLAQVFFCAANQINCPPAKDQVRRQEIIA
                     AGEAAKQAKLGIYSSRCQAQPVSSTSLVASTSANQATTSTKTATSSKTTTTSATITTS
                     NTSTNKTNTTSSQSSVDDWQPPETTIKTEVPAIVNTGTAQFILVASEPSTFECQLNDQ
                     SWKKCAADYELKNLTAGSYTLRVRAIDAAGNVDPTPAVFSWIIDKQRPTISWVTKPSS
                     TIAGSKVVFAVEVNEAAELLCDLDANGEEACPVNLTYDALSLGQHELTVRARDVAGNY
                     SAKLSASWTMVDNTPPDRVVIDYPPTNPWFTSSSSLTVSGSVEGGAKVMVNDRTAQSL
                     ADNKWRSAVSLSEGRNELTIVAVSSAGQSGPTTSLTIVVDKTKPSSSMVALPETVTES
                     GFVVNWLGEDLGPEPSDHLVFDVQYRLGSESWQSWLETVDYTSAIFDEPLNVGQKVSF
                     RVRARDLVGNWENWPANSAADTFTTLVAQPSGPPPKIVISQLVTRGTGGASDEFVELY
                     NPNDETVNLTGWRLQTKSAEGSTWINRLGSNGLPEGSYIEAHGYFLLASADYAGLTIP
                     DYQHESNWGLADSGGHVRLVDTQNNVLDKVGYNQASDPEGQPALADLAAGHSLERKAS
                     ANSTAASLGSGGSEANLGRGWDTDNNANDFVDQPAVRPRSGKHQIFNEDSLSAGLVHL
                     WHFNECLGNIIYDWVGQSNIVEQPFTWRVGPFDCAIYQTWERPAVNLTLTPVVDPAAV
                     TLAYWWRNASYPNEGRGHEYLVGADGQIILGLTPSAMGSVQFWHYGWYHIILDVLPQD
                     ANWHLLVATQENNQLKFYVDGDLKWTMATEYNPPQMITRLDLADENWPIDRDELAIWQ
                     RVLSPAEVRRLQQLSAPLSPRLNRPEQLEPVALYYWAFDEGMADTAYDNLHHVALTPI
                     KQWVYSPFNLGVRIEHPGDPQGQLPQALNNQDVSLSLWWRNSSYPNETRGDVILRDAN
                     GQGILGLVLGTYSLGIYFNGNRWYPVDKPINDGDWHQAVLVYNSYTYHLLLYLDGRLY
                     WQGENTWLTNSLASVSVGQENWLFDLDELTLWQGALSPTQVETMYELARPNLEEF"
     gene            5403..6425
                     /locus_tag="PK960_00830"
     CDS             5403..6425
                     /locus_tag="PK960_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07754.1"
                     /translation="MEIQGHQLHWLYIGNERVIRCWAAHLASRELAQSYILVGPADVG
                     KAVVARNFMRTLVCERRDQAGTDWPCEQCETCRQTARGIYPDFYELDVLPDKQKISID
                     QVRQLLDKIGLSSLANNYQVALIRHADKLSEPAANALLKSLEEPQTQALFILLVENLA
                     SLPPTIVSRSQVFRFYPVATNLIYDHLVSDWGANRELAKNLAQVCLGRPNLAIRFFEQ
                     PDLFQDYCQTAKILLQLITSSDLASRLAYLSQVYPVGAISAGEPEWRLWQIWLGLSRD
                     LLLIKQDQLPLIQHWFLRDELESAANTMSWSVLLKIFTNLGAAREYLIANVSPNLVFH
                     NLAVGV"
     gene            6430..7506
                     /locus_tag="PK960_00835"
     CDS             6430..7506
                     /locus_tag="PK960_00835"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HPD07755.1"
                     /translation="MSIFWQRTRYLFLGLLLLIGFSGCSTIISKPVNEAPDIGGVFRS
                     GNRGENWQPVNLIPTNTGQPGSIASLDANALIMDPSDHRALYLASVDNGLIFTYDEGQ
                     NWQVATSLGRIDVRAVAVDYYNKCLIFAASGNRLLRSEDCSRTWKQVYYDNSPDVVIT
                     SLLTDHYNSGAVFLGTSRGDIIWSNDKGETWQVVQRFDDVIKRIVMSPKDSRFMFAAT
                     ANKGLFRTRDGGATWENINQSLEEFQIGNQFRDLAITLEDQPVTYLATDYGLFKSVNL
                     GDTWTKIELLTPESQTVINALAINPHNSQEIYYVTNTTFYRSLDGGKSWSTKKLPTKR
                     AGKVLLVNPEKPEVLYLAVQKIKN"
     gene            7516..8655
                     /gene="tgt"
                     /locus_tag="PK960_00840"
     CDS             7516..8655
                     /gene="tgt"
                     /locus_tag="PK960_00840"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPD07756.1"
                     /translation="MFTLKKQSSQSGARLGLLTTAHGKVETPVFMTVATHGVVKHLTT
                     EELRELEVPIILANTYHLLLNPGIETLKQAGGLHHFMNWEGPILTDSGGFQVFSLTGR
                     RIHSHNLVKLTADGVYFNSPRDGRRYYLTPELSLDMQVASKVDIAVALDECLALPASL
                     AKVRQSVERTTAWAKRAKAHYLSLPEPKPLLFGVIQGGVSRKWREQSLSEVVALDFDG
                     YNIGGLSVGETTAEMYEVLEWLEPLLPHNKTRYLMGVGYPDNIVEAVRRGVDMFDCVI
                     PTREGRHGRLFFWQPDRHFIDETGKVREFYQIKNIRNSSFAQLDEPVNPYSRLPILRS
                     YSLSYLHYLYKIGEPLGCRLASLNNLEFYYDLMALIREAIKNNEL"
     gene            8660..9406
                     /locus_tag="PK960_00845"
     CDS             8660..9406
                     /locus_tag="PK960_00845"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HPD07757.1"
                     /translation="MEIMTVLTRQLQPPQDDSLGALIEGLDKIGGLKEGDVLAVTSKI
                     VSIYEGRAIAMSSIQDKDQLIISQAERYLPREMVPHNYAILTIKNHVLAPSAGIDESN
                     GAAYYILLPAEPENITRAWRNILLHRYNLSNLGLILTDSRSTPLRLGVVGIGLAFAGF
                     HPLRDYRGQPDLFGRPMKISQSNIVDSLAAVAVYAMGECAEQTPAAIIRGVDGLQFTD
                     DDCSQELWLDQSDDIYEPLYHNFLPGGGMK"
     gene            9408..10511
                     /gene="mnmA"
                     /locus_tag="PK960_00850"
     CDS             9408..10511
                     /gene="mnmA"
                     /locus_tag="PK960_00850"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPD07758.1"
                     /translation="MGGAKKQQKTQKIIVALSGGVDSAVVAQLLKEEGHEVIGVYLEL
                     WHDDYYHTAHQERGKEPWSDAQAICRKIGIPFYALNCAQEFKRRVVNYFLAAYNSGLT
                     PNPCVVCNKQVKFRRLIRYAQQIGFDAVASGHYVQLKGYSGHYELWRAKDKAKDQSYF
                     LYQLSQGDLAHLRFPLGELTKTEVRALAYSYHLPVASKKDSQEVCFINNDLPNFLQRY
                     LSLIPGPIVDSNGRVIGKHEGLPLYTLGQRRGIKIGGSGPYYVKGFDFLTNSLLVTNN
                     VNDPQLFRDTFFIPYLFWSSGRRPRRAIDCQVVIRYHHQPLDCQLIKETQGGWQVVLA
                     NPERSITPGQSAVFYEGDKLLGGGVIALPDSFC"
     gene            10589..12931
                     /locus_tag="PK960_00855"
     CDS             10589..12931
                     /locus_tag="PK960_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07759.1"
                     /translation="MATNTTSHFLKRASKISWPERVINWVLILTFFILPLFFINISWQ
                     GANLDKLFFLGLLVIIGWVVWLIKVLKEGALTWHWRKLDWLVLAILVVSAVATFWSPS
                     WHNSFFGGYGQPMRSLIFLVLLVLLYFLAVNNWSAKLRRSSWLAILISFSFITIYSLC
                     QLLGFYIIPLSFTRTISFNPIGSLSNLALFLAATLPLFIIALGSQEEFCERPSSNGKL
                     WWNIWLILTILANIAAIVILGSFNIWPVVILGLLIVLIFAISHLIKFTPLQLGFSIGM
                     LIISFVLLIVGNIGWPKVNLPSEVSLSRGFSWQIAQASLAKRPIFGNGLASFDNAFSR
                     FKSPDFNAAALWNLDFDVPTGWLAESLVIVGGLGSLLIVIGLVWALVLAGRRLLSSKT
                     ELPSADLKLGASLGAAVIVIIIGGLFIPVGNNLLLILGLLWILFMVVTYNWPRRRSVI
                     YWRKADERANAGWIVGFIAVAIFLLASLAYGVKIYAADILAAQSIKSQTTDQQIITMS
                     QAYNLAPWREMYGFGLAQLSWIKANELAQAAMTATGTPALEAQTAAKSYAQQAKAILD
                     QVSKLVNNQPANLKMAASLYEMIGDFEGAMATHQRLIELDPNNPWPYAKLAQLKVVQA
                     YQAQDKETKDKLVEEALSGYAKALELKPDWAEAYYYRAVLYQAIEKPVEATQDLVLAV
                     NNSGGATNYVLPLAQLLNERAKIETDSAANLRAQAQQLVEGVLATDDSNVNALYILAM
                     IYRDSGQTDLARETVNKLLDKAQEADKPFIEQQFADLLAK"
     gene            12986..14764
                     /locus_tag="PK960_00860"
     CDS             12986..14764
                     /locus_tag="PK960_00860"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPD07760.1"
                     /translation="MTLSSQELRQKFLDFFRSKGHAIIPSASLIPDNDPSVLFNTAGM
                     QPLVPYLLGEPHPLGRRLVNVQKCLRTDDIDKVGDTVHLTFFEMLGNWSLGDYFQEEA
                     IAWSWEFLTDKQWLGLPADHLAASVFAGDADAPFDRRSYELWQQMGLPEKRLAKLGQE
                     NNWWGLASHVGPCGPDTEIFYWTGPEPVPDIFEPANPFWVEIWNNVFMAYNEQPDGTF
                     IPLKQRNVDTGLGLERVLAVLNHEPDIYVSDLFQPAIHYLEKISGRLYSSETRTFRII
                     ADHMRASAFLLADEKAVTPSNKDQGYVLRRLIRRAVRLVYNWSIPPADILSGVIEQII
                     ATMGEAYPELVRQHDFIIAEVKKEIIKFNYTLGRGLKEFERLVGHHHFISGTDAFMLY
                     QSYGFPWEMTKELAQERGLAVDEAGFQEEMKKHQELSRTAAAGKFKGGLADNSLATTR
                     LHTAAHLLLQALRQVLGDGVYQRGSNITPERLRFDFSYPQKLTPEEIKQVEDIVNEQI
                     ALDLPVTCEEMSLEEARRAGAMGVFDAKYGERVKVYTIGSDRPFSQEICGGPHVQRTG
                     ELGHFRIIKEESSSAGVRRIKAILED"
     gene            14766..15773
                     /gene="galE"
                     /locus_tag="PK960_00865"
     CDS             14766..15773
                     /gene="galE"
                     /locus_tag="PK960_00865"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPD07761.1"
                     /translation="MVQPTLLITGGAGYIGSHAVKLFLDKGYTVVVMDNLSRGWQEPL
                     DILASRGKLIFVRGDLTASSDLATLFEHYHFAGVLHFAALCLVDESMSKPELYFDNNV
                     IGSFNLLEALRRYGPVPLIFSSTCAVYGESQYLPVDEAHPLNPANPYGESKLMVEKMI
                     KWYGERYRIPYVILRYFNVSGADADGLIGDSKKPSELLMQNAVRGALNIEPFTYTCGK
                     VATPDGTPIRDYINVEDLIEAHALAYDYLKNGGVSEIFNLGTGQGYSVKEIVQAVEHY
                     FGCTLKKKQGVVRQGEYAQIYADITKAKEVLGWRPRRSLEDSIVSLAKWYSRYPQGYN
                     F"
     gene            15933..16943
                     /locus_tag="PK960_00870"
     CDS             15933..16943
                     /locus_tag="PK960_00870"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPD07762.1"
                     /translation="MSYSYQPNIFKKQSHCYTNNAFTLVELLVVIAIIGILSTLSVVA
                     FNNVRAKARDSRRLSDVRQIGMGLEMYYDDKGVYPAPPTPTGTPITGLCLSNLGISST
                     CGAIVYLQKIPSDPLSNIHYTYSYLNSGESYRLGFNLEQGSGDWPAGTLAMGPNGISQ
                     DLLAANGIDWRDPNNWKKIPSSGSGCTTTYDQGRKAIKIENENRCFLAPASDPSYFPI
                     DTSRKYYIEAEYLTEGTTTYGFYLGTASYNASYILLPGHNGTYDYFGTSYDRPTSTNT
                     WTFISANKAIGGRPRTGTSTNRSLLDKWEPGTALARVLMIANYDTGAQTTYIRNIRFY
                     VE"
     gene            17195..17473
                     /locus_tag="PK960_00875"
     CDS             17195..17473
                     /locus_tag="PK960_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07763.1"
                     /translation="MANPSDFDWYEYTNLVSYGTFSPERLLPLNLTWNSNKGYGRIKT
                     SGVPGSMTNYGFLRGGYRESDSFAGVLALRLDLDSQYSDSATGFRCAR"
     gene            17697..17936
                     /gene="infA"
                     /locus_tag="PK960_00880"
     CDS             17697..17936
                     /gene="infA"
                     /locus_tag="PK960_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPD07764.1"
                     /translation="MTKANSTTNDSKSFLECEGEILELMPGLTFKVRLDNGHELLAHL
                     AGKMRINKIRLAPGDRVRLEISPYDLTKGRIIYRL"
     gene            17949..18062
                     /gene="rpmJ"
                     /locus_tag="PK960_00885"
     CDS             17949..18062
                     /gene="rpmJ"
                     /locus_tag="PK960_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPD07765.1"
                     /translation="MKVRASVKKMCKDCKIIRRFNRVRVICKNPKHKQRQG"
     gene            18077..18469
                     /gene="rpsM"
                     /locus_tag="PK960_00890"
     CDS             18077..18469
                     /gene="rpsM"
                     /locus_tag="PK960_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPD07766.1"
                     /translation="MTVRIAGVVLPNDKRIEVALTYIYGLGISKSRQILAKAKVNPDT
                     RTKDLTEDEANRLRILIEKNEVVEGDLRREVSSNIKRLKDIGCYRGIRHSRNLPVRGQ
                     RTKTNSRTVRGNRRQTMGSGRKPPAQKT"
     gene            18569..19015
                     /gene="rpsK"
                     /locus_tag="PK960_00895"
     CDS             18569..19015
                     /gene="rpsK"
                     /locus_tag="PK960_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPD07767.1"
                     /translation="MAELPEEVKKKLEKTKSSRLSVAKGKHRRKVERTLTKGNVYIKS
                     TYNNTIVTITDENGDVVTWASAGLAGFRGPKKSTPYAAQVITKIAVDRAKEMGLTDVQ
                     VFVSGVAAGREAAIRALNANGLMVNSIKDVTPIPHNGCRPRKPRRV"
     gene            19030..19665
                     /gene="rpsD"
                     /locus_tag="PK960_00900"
     CDS             19030..19665
                     /gene="rpsD"
                     /locus_tag="PK960_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPD07768.1"
                     /translation="MSRYLGSACKLCRREGTKLMLKGERCSSSKCALSRRNFPPGFHG
                     PQGRPRQSEYSLQLREKQKAKRMYGLLEKQFYLTFLNAKKQPGDAGENLLRLLEMRLD
                     NVVYRAGFADSRAKARELVSHAHFTVNGHPVDIPSYRVKPGDIIKVKSSRRRNQIFNG
                     LGERLLKKKFQAPSWLFLDPSELEVKVLHEPKEEDLEKNINTQLIVEFYAR"
     gene            19682..20497
                     /gene="rpoA"
                     /locus_tag="PK960_00905"
     CDS             19682..20497
                     /gene="rpoA"
                     /locus_tag="PK960_00905"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPD07769.1"
                     /translation="MEKIALPQKISVLPGNHPNEALITVEPLFPGYGTTVGNALRRVL
                     LSSLPGAAVIGVKIAGADHEFMGLPGIAEDVLTIILNLKKLRLRIFSEEICKLSLEAS
                     GQKVVTAADITPNSQVEIINPDLVLAHLTSKDANLSMEIFVSRGRGYEMVENRPKVKR
                     EIGYIEIDSIYTPVVAAGLSVENVRVGQMVNWDKLVLRIVTDGTMTPRQAFIDASKVL
                     IDQFTAIVDLAEVEPELEGELAVNGEKKPSKVKVSAKKSKKGTTSKSKISKAK"
     gene            20499..20846
                     /gene="rplQ"
                     /locus_tag="PK960_00910"
     CDS             20499..20846
                     /gene="rplQ"
                     /locus_tag="PK960_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPD07770.1"
                     /translation="MRHRNKGVILDRAKEPREMMLRNLAASILMHEFVVTTKAKAKAV
                     QPLVEHVLTIAKAGDLAARRRLIAILPQDKAVSKALELAKRYESRNGGYTRIISLGCR
                     QGDGAQQVRLELV"
     gene            20856..21206
                     /gene="rplM"
                     /locus_tag="PK960_00915"
     CDS             20856..21206
                     /gene="rplM"
                     /locus_tag="PK960_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPD07771.1"
                     /translation="MVQREIYTIDASGKTIGRLASQIALLLRGKNKASYKPQVDEGGV
                     VYVKNIREVVFTGKKLEQKKYYRHSGYPGGLKSKAMSQVQAEQPDEVLRRAVKQMLPA
                     NRLREAMLKRLIIN"
     gene            21286..21705
                     /gene="rpsI"
                     /locus_tag="PK960_00920"
     CDS             21286..21705
                     /gene="rpsI"
                     /locus_tag="PK960_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPD07772.1"
                     /translation="MDYSTTREFTGKYLRAVGKRKTASAQVRLYDKGSGIIVVNGQAL
                     ASYFPVWSDQAAVIQPLRLSGLQKNLDFSIVVRGGGKTGQAQAIAHGIALCLLKLNPD
                     LRSVLRAKGLVTRDARIKERKKPGLKRARRAPQWSKR"
     gene            21839..23284
                     /locus_tag="PK960_00925"
     CDS             21839..23284
                     /locus_tag="PK960_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HPD07773.1"
                     /translation="MPARVTNIAKNTSYFTLALVIQKLISLTYFTLYARVLGPADLGK
                     YYLAISLTSIFAIFIDIGLSNVLTREVAKYPDKASNWLGTVLAIKIPLAIITTLAVVL
                     FVNILGYPLLTKELVYISIISMVLDSFTVTFFAVSRGFHNLKFESVSSVIVQSIILVV
                     SLMVFHYGGGVRWLMFSLVLASSFNFCYSLFVLWRYWRLPVRPLWRRTWIKKAILLAT
                     PFAGYGIFQRAYIYLDTVLLSKISGDVATGIYQIPFKIINALQFLPMAFIASLYPALS
                     LYWQENREQLSITFNRAMHYSAIISLPLTIGGWVVAEPLVAIFSSSYTGAVWPLRLCL
                     IALPFMFLGYPIGSLLNACDEQRANTRNMFIVLITSVVMNLLLIPPLGAVGASITVVI
                     TNGLLVLLGLISAKKIAPHLWRWLKQFGQTILASLIMGVIVWLLRPYCNVFVVIIIGA
                     LVYGLALLLTGSLTKADVQSVIQSFRRRPTN"
     gene            23290..24381
                     /locus_tag="PK960_00930"
     CDS             23290..24381
                     /locus_tag="PK960_00930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPD07774.1"
                     /translation="MRCLLVTLEYPPFYGGIANYYGHLVKAWPQADEVTVLDNNKGQL
                     LAPQGKWPWRRSLVSLWRALKQGHYDFVLVGQILPLGTVVWLLSFIFPIKYGVFLHGM
                     DWGEALKRPRKSWLAQRILRRSALIIAANNYTADLVKGTMPLLSSRIIVVNPGIDSVE
                     PPLVAPEYLASLRQRYNLEGCQILLTVGRVVERKGVDKVLEALPQLLPYYPKLRYIIL
                     GNGPYLPVCQQLMADLGIKDEVIFITNATDEEKAAWYQLADIFIMPARAKDGDFEGFG
                     IVYLEANQAGKPVIAGQSGGVGDAVVDGVTGLMVDPQSPRAIAQAVRRLLNDESLRRQ
                     LGKNGQARLQQFLWPRQAHLLRDKIKEVL"
     gene            24378..25088
                     /locus_tag="PK960_00935"
     CDS             24378..25088
                     /locus_tag="PK960_00935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPD07775.1"
                     /translation="MISVIIPVYNQADKIGRCLDSLLAQTYRDFEIIIVDDGSTDNLD
                     EVLLAYETKLQDAKIPYKIIVQENRGANAARNRGWAEAQGDFLLFSDADITWRPDALE
                     KLVNSLIHNPQASYCYSSFRLGRKKFHLWPFDPERLYREPYIHTSALIRRADFPGFDN
                     TLKRFQDWDLWLTMLEQGKKGLFYDDFLFTVASGGRISKWLPSFAYKIFPFLPKVKKY
                     QEAAAVIKAKHHLPYETK"
     gene            25075..25551
                     /locus_tag="PK960_00940"
     CDS             25075..25551
                     /locus_tag="PK960_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPD07776.1"
                     /translation="MKPNRHLWWLIIIILIIIGIIVYGLWRANHRGPRFMADSELVLP
                     DGSQVQIALLTTKAEKYRGFSGMRQPCPDCGLLFVWPALYQPVIVMRDMLFPLDLVWL
                     RDKQIVQLTLNIQPEEGEESELTKYEPSEPIDAVLEVPAGFAGRHNLKIGQFVDWH"
     gene            25562..27064
                     /locus_tag="PK960_00945"
     CDS             25562..27064
                     /locus_tag="PK960_00945"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPD07777.1"
                     /translation="MLKSVSWTRVRYILEAIIIVELLSALTYFCPTIGPWVFILAMLA
                     GLYLAIVRLDWAIYALLIDLMIDSKGYLLSLSLGGFRLSWRIGLWLIIMVVWLIKELI
                     ALRQHRTSLALEWHKFKSSGMVIFLLMLVWGIIIGLIRGNSLSNLFFDANGWLFVLLI
                     GPLLSIKKWPSQEWSAVLTAGMAWLITKTSLLLYVFTHLSTIWQAIIYRWVRDTQVGE
                     ITRMSAQFYRIFLQSHIYLLILVVIASWLLATKLPRTQWEKWLVKDKGYWWIIGLGLA
                     LMPIIVGSARTFWLALGVLMLFIFILIARRYHWSIAWRWLITMILAGFVALISIFIIV
                     SFPWPKPQVSFNPGDIVEERLGDLANEAGASSRWSLLPAMLQGISKAPLLGHGFGATI
                     TYISADPRVLAQNPSGSYTTYAFEWGWLDIAFKFGLIFALWYLVWLIWLISRAWRHGL
                     TGEIWALGLGTLVVVHFFSPYLNHPLGIMWLVGLIAWLSSHKQYESLSSS"
     gene            27042..28121
                     /locus_tag="PK960_00950"
     CDS             27042..28121
                     /locus_tag="PK960_00950"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPD07778.1"
                     /translation="MNHYLLASLLAFILSAALTPLIKQLAWHWHIVDRPDQVRKLQTK
                     PVALLGGWAIFIAASLVMWLFRDYLIAGDLTGRHWLGVWIGGLILMIGGTLDDLYNLR
                     PSAQLVFSILAVLAVIIGGVGINKVTGLNGELIFLNKWQISLFTWQGITRHFVVLADL
                     FTLTWLMALMYTTKLLDGLDGLVTGLIGIGSIIIALFTLTTKYYQPDIALASLIFAAA
                     CAGFLLYNWSPAKIYLGDGGSLLLGYFLGVLAIISGGKIAIALLIMGIPMLDVAWVIV
                     RRLLNGENPFKSADRGHLHYRLLDLGWGVRRSVLAYYLIALVFGVSALFLQSRGKRLA
                     LLLLFLVMAIVVVYFSRLDKARKRP"
     gene            28172..28483
                     /gene="rplU"
                     /locus_tag="PK960_00955"
     CDS             28172..28483
                     /gene="rplU"
                     /locus_tag="PK960_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPD07779.1"
                     /translation="MSKLAIILSGGKQYKVKPGQIIKVEKLASEPGTEVTLETLLLAD
                     ETSGEVQVGRPFLENGVKAKVIEHSRAPKVRVVKYKNKTRYKKTTGHRQPFTKIEIAE
                     W"
     gene            28587..29783
                     /locus_tag="PK960_00960"
     CDS             28587..29783
                     /locus_tag="PK960_00960"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HPD07780.1"
                     /translation="MTSQTNQSADSIFTSNDFSLFKLVNKLLQKLNPREREVLRRRFG
                     LNNPHKETLEHIGSTYGLTRERVRQIEAAGVKKLQKLRDMEKQLILLRDALDDLLADN
                     GGLMDKEYLESSFLVLSHESLQESQRPIYRNNFDFLVSKVLADDFDPVTSDLFNDSVK
                     LKYQAIDHLEKLAQELVEQVRQTRRLFLLEDLIKLSFELDAFKENADKLVSTNKFDLA
                     SALAGYENFQDGELINKYKPIYSVLRATRHLEENKFGVWGEADLPEIRPRTVADKIYL
                     VLKDAGQPLHFREITERINRVFTDKPANSATVHNELILDDRYVLVGRGIYALKESGYQ
                     AGRVVDIVSRVLRQARRPLSKEEVIKKVLEQRLVKPATINFILANKELFQKLPDGTYT
                     LKESAV"
     gene            29843..31393
                     /locus_tag="PK960_00965"
     CDS             29843..31393
                     /locus_tag="PK960_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07781.1"
                     /translation="MNLTPLINFFYLPADEIVWRLVFYYGWMIPAIMFLKGSLMVWLS
                     WRRRLFFSKQHFTLLAIDIPRGNEQTPKALENMLAYLAGAHSNPNLIETYWEGKCQLG
                     FSLEVVGIDGYIQFLVRTPDSFRNLVETAIYAQYPDAEIAEVEDYVDTVPDNFPNPDF
                     DMWGAEFVPVAMNPPYKQALPIKTYKDFEYDYGRPEYHFKDPMASLMDLMSSLKPGEQ
                     IWYQILLIPTVADWPALGQEAINNILGNKPKSKETIIEKFFDGILNLISQIGDFVFDG
                     ILNLISQIGDFVGGIFGLGGTSSQTSDKKEEKEDQFKMMNLLPIQKKQIEAIQDKVSK
                     IGYLVKIRMIYVARREVMNKAKAVNGFVGFIKQFNTSDLNSLKPDSKVTQTSADYFNR
                     LNIINSRKNKIMQAYKARDDSAGRSPWYLSVEEIATLWHFPIESVVKAPLLQKASSKK
                     MEPPMALPTEETNEEEIELDQLFTLDSEDELTPAEPSENSKSKEVNTDERDNTFHQAP
                     PPSNLPGI"
     gene            31412..33031
                     /locus_tag="PK960_00970"
     CDS             31412..33031
                     /locus_tag="PK960_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HPD07782.1"
                     /translation="MDEDVTLFGETTFRNQNHKFGIKLDDRRRHMYVIGKSGMGKSTL
                     LENMIAQDINKGRGLAVVDPHGDLAEKIIHYIPDKRIEEVVYFNPADAEYPIAFNVVE
                     QVDPRLRHLVASGLIGVFQKLWADSWGPRLEYILRNAILAVLDYPDSTLLAITRMLSD
                     KNFRKRVVEKIQDPVVKAFWVNEFSSYNDKFASEAVAPIQNKVGQFLSSSLIRNIVGQ
                     VRSSIDLRDIMDNGKILIMNLSKGRIGEDNSALLGSMMITKLQLAAMSRVDTPEEERR
                     DFFLYVDEFQNFATDSFANILSEARKYHLSLILAHQYIDQLTDSVRAAVFGNVGTLIT
                     FRVGATDAEFLAKEFLPLFTEEDLVNIPKFNFYLKLMIDGITSDPFSARTLPPLSKDE
                     QTNNVDKVIEYTRSHYANRRDIVEEEIMRWHLNQGGVAERENRVVNVLEQPEVLNSTT
                     KMDVLEQRFPAVCDGCGQPTTVPFKPDGQRPVYCPDCLKRYRAERSERQRLLQSSPSQ
                     LDHLEGASLRQIHRPNISKNNNKGNNGQSSH"
     gene            33012..34610
                     /locus_tag="PK960_00975"
     CDS             33012..34610
                     /locus_tag="PK960_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD07783.1"
                     /translation="MDKVAIDYLKIGRASELTGRDRRLYRFLEILPGLLSWLTLLVLI
                     IFSYFQPVWVAIFVIAFDVYWLLLVVYLALILISAYRQITHNLKVDWRAQCLALPPVD
                     LVYRPLQGEPIVSSGVRWNDLYHLVIFPCYNEGYNIIQPSIEALVKSPFPRDKMIVIL
                     AIEERGGPAIQAVAERLRQEYENSFFKFRVIVHPDNLAGEIKGKGANQAWAAKKAKEE
                     IIDPLGIDYNLILASVFDSDTVIYENYFYCLSYKFLTTKNPYRRSYQPIPMYHNNIWQ
                     APFFARVAAYSNTFWQMMQQIRQEKLATYSSHSMPWRALVEIGFWSTKMVSEDSRIFW
                     HCFCYYRGDYEVEPLYYPVSMDVCMDETVWQTARNLYKQQRRWGWGVENVPYLIFNTI
                     KEWHVIPKKVFLDKIFIQFYGFHSWATNAIIIGCIGWLPLFLGGDRFNQTVLSNNLPN
                     VTRILMTIAMVGMVLSAIISSLLLPPRPAKTPWWRKLGMIAQWIFLPVTIVVFGAIPG
                     LEAQTRLMLGKYMGFWVTPKARSG"
     gene            34737..36137
                     /gene="cysS"
                     /locus_tag="PK960_00980"
     CDS             34737..36137
                     /gene="cysS"
                     /locus_tag="PK960_00980"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPD07784.1"
                     /translation="MKLRLHNTLSRQLEVFKPQQPPVVGLYTCGPTVYNYAHLGNLRA
                     YVFSDLLKRTLLYNGYEVKHIMNITDVGHLTDDADAGEDKVEIRAEREGKSAWEIADF
                     YIKAFKDDLQALNIIPPTTYCRATEYIQEQIELAQILEQKGYLYRISDGLYFDTSLVP
                     DYNKLSHLPLEKLKEGARIDKNPEKRHPTDFAVWKFSPLGQKRQMEWDSPWGKGFPGW
                     HLECAAMSLKLLAGHLDIHTGGVDHINVHHTNEIAQAEAATGKPFFHYWLHNAFLNIA
                     GGKKMAKSGANFLTLKVAVIDEGWLPLAYRYACLEVHYRKPMEYSKASLAAADQALKG
                     LYREVAALGGEMGEVSPLFREKFVEAINHDLNSPQALAVLFEVLRSQLSNKDKLATIK
                     NFDEIFGLDLIGGGQHYSEMLTNIPPAVQALFNRRQLAREAQDFPLADELRRQLAEAG
                     YGVEDGPGGSRLTPLF"
     gene            36140..37078
                     /locus_tag="PK960_00985"
     CDS             36140..37078
                     /locus_tag="PK960_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HPD07785.1"
                     /translation="MDNFWAHLARPFTVLAPLSGFTDRAFRQICRSYGADVVVSEMAS
                     VAALVHSPEKALERLYFNEIERPYVVQLFGADPNQFGEAARLVTNRIQPDGLDINCGC
                     PAPKIIKEGAGAALGADLPRARQAVQAVLSNTSLPVSIKLRAQNKDISAQQVVAYLAD
                     LPLAAVTVHGRSVSGGFSGPVDPGAIALCRPYVAGVLIANGGIVDLMTAQIMLQKSLA
                     DGIMIGRGALGRPWIFQEIKEGREINLTWTELTELIRHHAQLIVAYKGEVGLIEFRKF
                     LAWYVKGWPQAKALRTQLMQIKTLSELENVITSVSA"
     gene            complement(37056..38027)
                     /locus_tag="PK960_00990"
     CDS             complement(37056..38027)
                     /locus_tag="PK960_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HPD07786.1"
                     /translation="MGQYLKISPKIEELVIDNPKTNGAVNWININSAGKTEIEYLLKR
                     QHFNLHHLKQSLSRSIAQRAIIEKEKDYIFIVLHFPIIKNGLIMPSEIDFFMGHGYLV
                     TVHNGDLPPLNNFFNLCRKSENSTLAYELESSSILLYEILEQLMHYCYEILDEHSKRI
                     ATVQDIVFKVDSNQRETVQELLTLRNNIIKIRRILQNHKNILQTLTTIKSSLVPATEL
                     KKYYSTLVEHSKRIWENLDNQKEMIEVLHDTNESLLNDQINQIMKTLTIFSVIILPLN
                     LIAAIFGMNTIIGMPFINNRYGFWCVIIIMLLPTAIMFLYFKRKHWL"
     gene            38138..38608
                     /locus_tag="PK960_00995"
     CDS             38138..38608
                     /locus_tag="PK960_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07787.1"
                     /translation="MPQERLRSNKVEQPLTSPEVEQLASQEKKKEGVPLPSPEKPESA
                     AVSASINSPSAYLTGSIAPLTPRQAAIDDILEDGLTDIYLSLPPDKQRELKEVGEQTV
                     RQIDSLLDHVKLQWRKIMNLIRRFLSIIPGINKFFLEQEVKIKTDRIIALKDKE"
     gene            38611..41082
                     /locus_tag="PK960_01000"
     CDS             38611..41082
                     /locus_tag="PK960_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HPD07788.1"
                     /translation="MDPLIILGGVLLTAIIVWLVVFIWRTIVWRWYQAHAYFDQVVYL
                     VKLPKDRPKDQNQDFTAQQMREEISCAETIFASIGGLRAQRGWAVWLWGRDDHFSFEI
                     VANSSIIAFYVVAPRRMARYLEQQINAHYPDAVLEEVIDYNMFTAQGAIAIGSLKTTK
                     HYALPLKTYNQEEVDLMNSQLNVMSKLSPGESLAIQYTVRSAYGSWHGAVKKIVQLAY
                     KKKSVSAALQTSIWSSIADFWSDAFSSNKAKKEEAAAKQAEKRLTAMEEEMLKLMEQK
                     NSKAGLDVNLRLVACGNNQGRADALLGDLANAMSQYNYYEYGNSFQAKPRRKPVKNLI
                     KQFIYRRFDEKSKFLLNTEELASLYHFPLRNAETPNILWLSAKQASAPSDVPTEGLLL
                     GYNVFRGIRREIHIKEDDRLRHVYMIGKSGVGKSVLLANMAIQDVLNGQGLCVIDPHG
                     DLVNDILARVPAERAEDVVLFSPADLERPMGLNLLEFDPKYPEQKTFVINEMIGIFDK
                     LYDLRATGGPIFEQYMRNAMLLVMADPGSGSTLMEIPRVLADADFRHMKLGRCADPTV
                     VDFWRKEAEKAGGDAALANVVPYITSKLTSFISNDMMRPIIAQQKSAINFREIMDQHK
                     ILLVSLPKGLVGEMNAYLLGMILVGKMLAAALSRADMPKEERCNFFLYIDEFQNFTTN
                     SICQILSEARKYGLGLIIAHQYIGQLVNRGDTAIKDAVFGNVGTLISFKVGSEDAEVL
                     QKEFAPTFDAYDLINVEKYTAYIKLLIDNMSSRPFTMKTPWPLLGHYDQEMAEKIREL
                     SRLKYGQDRSLVEAEIIQRSRMNLN"
     gene            41084..41746
                     /locus_tag="PK960_01005"
     CDS             41084..41746
                     /locus_tag="PK960_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07789.1"
                     /translation="MAQEPSIINKEGDEQEWQPDQTISFSDQASEIPSVLPPATGSQP
                     LKDNRDEGYLLSGKQIKILQSIAKKLTTLAADLNHLVQSLMASNTSEEVLAGQSGLTI
                     EGYFNGEQMVASNGQVYEVPSNYASKSKLVEGDNLRLTIDSNGQFLYKQIGPVARRRL
                     VATLQQGSDGNYYAVHGEQRWRLLKASVSYFRAEAGDRVTILIPKDKESQYAALENLV
                     LE"
     gene            41748..42200
                     /locus_tag="PK960_01010"
     CDS             41748..42200
                     /locus_tag="PK960_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07790.1"
                     /translation="MMVGRWSSRKILHYFPSLTAEQESLLSGQPYFIHPCLLLKIADR
                     QHNFSTISHLPSNKQVRMAFETQASLLPMKEILDYDCKGLTISEAAANWQQFLDKGHF
                     SSLSALKKELYKKQFVDLDSTMYNDHANAIIGQLTDKFVKAMCIIVCK"
     gene            42233..42646
                     /locus_tag="PK960_01015"
     CDS             42233..42646
                     /locus_tag="PK960_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07791.1"
                     /translation="MASINSLISDLELDKSLIDCLKNRDLDQKFFYTEEGADLYYSSY
                     EHSAKLVPVDFSLPKYSVGAILFNFFFLFNKKVVIKARNEVIHFLSDKRLNLLNIRAY
                     YPDTLNNFLKEHGFQLVQSKVDQNIKHGFFIYQRV"
     gene            42650..44242
                     /gene="dnaX"
                     /locus_tag="PK960_01020"
     CDS             42650..44242
                     /gene="dnaX"
                     /locus_tag="PK960_01020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPD07792.1"
                     /translation="MSTLYRQYRPQTFAEVVGQNHIKITLAEEIKRHQIASAYLFCGP
                     RAVGKTTIARLLAKSLNCLNRPDNSAEPCNNCANCLAITAGQSLDIVEIDAASQTGVD
                     NVRENIIASSRLAPSQGKYKVFIIDEVHMLSTSSFNALLKTLEEPPEQVIFILCTTEV
                     HKIPATVISRCERFDFKKISQADIIKKLHYILKQEGWSADEAVLTAIAKNSQGHLRDA
                     ESLLGQLMAVSQPMKNGELHLTMEDFYLLAPSSDLVIISQFITALSEQNSVEAITIIN
                     QLVADGRDVRVFLQDLIETLRQLLLSKINPQLLVEYQGQWGEDDMFGLLKLRQKLSIN
                     QLVQFLQLFIQAFNETKEAFIIQLPVELAIVKALGPSEIEKVSKIEKPTNPPESPSND
                     SSPLKKSGEENKKKNEVMAPLLSEELLSRWPAVVNEIKNHNLGLSVVLKDCRPQIEGD
                     QICLVFSYKLHQDQVKPLPIKHLIEEVMAKVYGRQLTITTKLLSESRPAESAVPQNKS
                     NKTEEGASLDSLLEIFNGQIVS"
     gene            44669..44739
                     /locus_tag="PK960_01025"
     tRNA            44669..44739
                     /locus_tag="PK960_01025"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44701..44703,aa:Gln,seq:ctg)
BASE COUNT        12379 a         7516 c        10304 g        14593 t
ORIGIN      
        1 aacgtttaag gggggagcgt taattctacg ctagaccgac cgggcgactt aataaatacc
       61 ccgctatcat atgattcata agttgaaaaa aatattgtct cagccttatt tttggttaat
      121 tattgggatt atcttggtta tcgttatttt ggggctaatt gtggggccaa ctattttaga
      181 aaaagttgag tggcataaat ggcaggcgcc attttttaat gatgcacgta ctagcttggg
      241 cggagagagt ccggaggaag cttatcagaa gttttggcag gctatgcagg ctaatgaacg
      301 tgacactgct ttagcttatg ttattggagt aaggcgacca tattttcaga gagcctggga
      361 agacccagcc ctgtgggccc tagaccaaag tttacccgag acgactagtt tagtctttat
      421 cggtgattgc ctgcctgatg ctatagcctg taaaaagcgg gcagttttaa gttattccac
      481 tgcgactacc actggccgga ttgaacttta ccagaattta gccggccggt ggcagattgg
      541 tgatatttat taattttatg ttaaggaggg gaaagagcca tttacggctt caatattttt
      601 tattattttt attactagga tttgtaggcc ctagagcagt actcgcctat gaccatcaga
      661 ttactcatcc acgatttact agttggtcag ttcagatata taatttagct tttcgtccag
      721 taattactaa tcaagaatta gggtggctgg cagccggagc ggcagctgaa gatcaaccag
      781 ttttaagaac gatgaatcat ttttggtggc cccagactga ccgcccccta agtgctggga
      841 gttatcattt aatgtcttta gaaaccgcgc cagattgggg gttagcgcca ggtgcgcagg
      901 ctaatttaat ttatgggcca tcttttagct ggtaccaagc aattgaggct tatcggcgag
      961 gagattatga gacggctttt gttaatttag gccatgtgct tcatctttta caagatgtta
     1021 gtgtcccagc ccatagccgt aatgaccccc atgtcttagg agacgcttta gaaaattggg
     1081 tttttcagca tcatgaggag ttggcggttt taccagccct agtgcggcca cggtgtcagg
     1141 attatcgtga ttgctttcga caaatggcca gtgcagttaa tgttaatttt ttgagccaag
     1201 atacaattaa tagtccggcc caagctggcg attggccgga taaaccagta attaaagatt
     1261 ggccttctga gagtggatat gttatttatc ggggatatag actagtttat tataatgaac
     1321 cacgacgtcg ttttatttta gacgatatag tccttagtga ttattggtcg cagattgcgc
     1381 ccctgactgt tggttatgga gcggaattac tgcgtctctt ttttacagct gtggctgatc
     1441 cccaggttgt gccggagaca acttcttggt ttactcgctg gcgttcaacc attgaggctg
     1501 gcagtggagc cccggtagag attatagaca acccagctaa aattactgac aatttaacct
     1561 catctccggc tatagtttcc tctagtttaa ttagccctgg gtccaccatt acttctagtg
     1621 tggcaataga tagtaaatct aactctacta gtactccaac cggagttaag acgaaccaag
     1681 ctagtaccag tcaagcctta atagctcaag ggcttaatgt ccaacttaaa agagtaattg
     1741 atggtgatac cattgagctt actactggcg agaaagtgcg atatttaggg gttgatgcac
     1801 cagaattagc ttctacttct tctagggcca gtgaatgtct agctgaagaa gctaaaagtc
     1861 gcaatcaggt cttattaact aggggaccat tgactctaat agccgatagt ggtggcgata
     1921 aagatagtta tggccgttta ctgcgttatg tttatgctgg tgatatattt gttaacaaaa
     1981 cattggccgc tgaaggatta gctcaggtat ttttttgcgc agctaatcaa ataaattgcc
     2041 caccggctaa agatcaggtg agacggcaag aaattattgc tgctggtgaa gcggctaagc
     2101 aggctaagtt aggtatttac agcagccgct gtcaagctca accagtttca tctacctcgt
     2161 tagttgctag tactagcgct aatcaagcta caactagtac taagacggct acgagttcta
     2221 aaacaacaac tactagtgct actattacta ctagtaatac tagtactaat aagacgaata
     2281 ctacttctag ccagtcaagt gtggatgatt ggcaaccgcc agagaccacg attaaaactg
     2341 aagtaccagc gatagtaaat acagggacag cacaatttat tttagtggct agcgagccaa
     2401 gcacttttga atgtcagctt aatgaccaga gctggaagaa atgtgccgct gattatgagc
     2461 ttaaaaattt aactgccggg tcttatacct taagagtgcg ggcaatagat gcagcaggca
     2521 atgtcgatcc cacaccagct gtcttttctt ggattattga taaacagcga ccgaccatta
     2581 gttgggtaac taagccatct agcactattg ccgggagtaa agttgttttt gcagtagagg
     2641 ttaatgaggc cgctgaatta ttgtgtgacc ttgatgctaa tggagaagaa gcctgcccag
     2701 ttaatttaac ttatgatgcc ctatctttgg gccaacatga actaacggtg cgggctcgtg
     2761 atgtggccgg caattattcg gctaaattaa gtgctagctg gacaatggta gataatacac
     2821 caccagacag ggtggtcatt gattatccgc cgacaaaccc gtggtttact agttctagct
     2881 ctttaacggt gagtggctcg gtggagggtg gagctaaagt tatggttaat gaccgcacag
     2941 cccaatcttt agctgataat aaatggcgtt cagctgtcag tcttagtgag ggtcgtaatg
     3001 aactgaccat tgttgcggta agttcagccg gacaaagcgg gccaactacg agccttacta
     3061 ttgtggtaga caaaactaaa cctagctcta gtatggttgc cctgccagaa acggttacgg
     3121 agtcgggatt tgtagtaaat tggctggggg aagatttggg tcctgaacca agtgatcatc
     3181 tagtatttga tgtccaatat cgcctaggta gtgaatcatg gcagtcgtgg ctggagacag
     3241 tagattatac tagcgctatt tttgatgagc cccttaatgt tggccaaaaa gtttctttcc
     3301 gagtgcgggc tcgagattta gtaggtaatt gggaaaattg gccggctaat tcggctgctg
     3361 atacttttac taccctagtg gctcaaccaa gtggtccgcc accgaaaatt gttattagtc
     3421 aattggtcac tcgtggtacg gggggcgcga gtgatgagtt tgttgagtta tataatccta
     3481 atgatgagac cgttaatttg actggctggc gattgcaaac caagtcagct gagggctcaa
     3541 cctggattaa tcgtttaggc agtaatggct taccagaagg cagctatatt gaggctcatg
     3601 gttatttttt gttagccagt gctgattatg ctggtctcac tattcctgat taccaacatg
     3661 agagtaattg gggactggca gatagtgggg gccatgttcg gttagtggat actcagaata
     3721 atgtgttaga taaagtggga tataatcagg ctagtgatcc ggaaggccaa cctgctctgg
     3781 ccgatttagc agctggtcat agtttggagc gtaaagctag tgctaattcc accgctgcca
     3841 gtttaggttc aggcggcagc gaagctaatt taggtagagg ttgggatact gacaataatg
     3901 ccaacgattt tgtggaccaa cctgcagtaa ggcctcgttc aggtaaacat cagatattta
     3961 atgaggattc cttgagtgca ggattagttc acctgtggca ttttaatgaa tgtttgggca
     4021 atatcattta tgattgggtt ggtcaatcta atatcgttga gcaacccttt acttggcggg
     4081 ttggtccctt tgactgcgct atctatcaga catgggaacg accggcagtg aatttaacat
     4141 taacaccagt cgttgatccg gcagcggtga ctttggctta ttggtggcgt aatgcctctt
     4201 atcctaatga aggtcgaggt catgaatatc ttgtgggtgc ggatggccag atcattttag
     4261 gacttacccc ctcggcaatg gggtcggtcc aattttggca ttatggctgg tatcatatta
     4321 ttttagatgt tttgcctcag gatgctaatt ggcatcttct agtggcgact caagaaaata
     4381 atcagcttaa gttttatgta gacggagatt taaagtggac catggctaca gaatataatc
     4441 ctccgcagat gattaccagg ctggatttag ctgatgaaaa ttggccaatt gatcgtgatg
     4501 aattagccat ttggcagcga gttttaagtc cagccgaagt acgtcgtctc cagcagttat
     4561 ctgccccctt aagccctcga cttaatcgtc ctgaacaact tgagccagta gctctttatt
     4621 attgggcttt tgatgaaggt atggctgata cagcgtatga taacttacat catgtcgctc
     4681 ttactccaat taaacaatgg gtgtatagtc cttttaattt gggggtacga atcgaacatc
     4741 ctggtgaccc tcaagggcaa ttgcctcaag ctcttaataa ccaagatgtt tctttgtctt
     4801 tatggtggcg caatagttct tatcccaatg aaactcgagg tgatgttatt ttacgtgatg
     4861 caaatggtca gggtatatta ggtttagtac ttggtactta tagtttaggc atttatttta
     4921 atggtaatag gtggtatcca gtagataaac caattaatga tggtgattgg catcaagcgg
     4981 tgttagtcta taattcttat acttatcatt tactattgta tctagatggt cgtctttatt
     5041 ggcagggaga aaatacttgg cttactaatt cattggcttc tgtgtcggtg ggacaagaaa
     5101 attggctgtt tgatcttgat gaattaactt tatggcaggg ggctttaagt cctactcagg
     5161 tagagacaat gtatgagctg gctcgaccta atttagaaga attttaattg ttttgttatt
     5221 ggccttttgt atacgattat agcttaacta gttttatgtc taagggcatt aaaactcctt
     5281 tgttgggggg ttaattggtg tttgggctct ttatggtttt gagactgtta ttattgctcg
     5341 gaaaaaatag ggggaatata ttatttgcca attgacttta cccgtgataa tctaatagct
     5401 gtatggaaat tcaaggccat cagcttcatt ggctttatat tggcaatgag cgggttattc
     5461 gttgctgggc cgctcattta gcttcgcgtg agttagcgca aagctatatt ctagttggtc
     5521 cagctgatgt aggtaaggca gtggtagcta gaaactttat gcgtactttg gtctgtgaac
     5581 ggcgtgacca agcgggcaca gattggccct gcgaacaatg cgaaacttgt cggcaaacag
     5641 cgcggggtat ttaccccgat ttttatgagc tagacgtatt accggataag caaaaaatta
     5701 gtattgacca agtacgtcaa cttttagata aaattggtct atcatcatta gccaataatt
     5761 accaagtggc tttaattcgt catgctgaca agttaagtga accagcagct aatgccttac
     5821 ttaaaagttt agaagagcct caaacccagg cgctttttat tttattggtg gaaaatttgg
     5881 cttcattacc ccccactatt gtttctcgta gtcaggtatt tcgcttttat ccggtagcga
     5941 ctaatttaat ttatgaccac ttagtaagtg actggggggc gaatcgtgaa ttagctaaaa
     6001 acttagctca agtttgtcta ggtcggccca atttagcaat tcgctttttt gagcaaccag
     6061 atttattcca agattattgc cagacagcca aaattctatt acaattaata acaagttcag
     6121 atttggctag ccgtttggct tatctcagcc aagtttatcc cgtaggagct atctcagccg
     6181 gggagccaga atggcgtctg tggcagattt ggttgggact tagtagggat ctattgttga
     6241 ttaagcagga ccaattgcct ttaattcaac actggttttt gcgcgatgag ctagagtctg
     6301 cggctaatac tatgtcctgg tcagtgttat taaaaatatt tactaattta ggcgcagccc
     6361 gtgagtattt gatagccaat gtttcaccta acttggtttt tcataactta gcggtcggcg
     6421 tataatttta tgtctatctt ttggcaacgt actcgttatt tatttctagg actactttta
     6481 cttattggtt ttagcggttg ttcaaccatt attagtaaac ctgttaatga ggccccggat
     6541 atcggtggag tgtttcgcag cggtaaccgt ggtgaaaatt ggcagccggt taatcttatt
     6601 cccactaata ctggccagcc aggtagcatt gcctctttag atgccaatgc gcttattatg
     6661 gatcctagtg accatcgggc attgtatttg gctagtgtgg ataatggctt aatttttact
     6721 tatgatgagg gacaaaattg gcaagtggct actagtttgg gacgtattga tgtgcgggcg
     6781 gtagctgttg attattataa taaatgtcta atttttgctg cttcaggtaa tcgtttattg
     6841 cgctctgagg attgtagtcg cacctggaaa caagtttatt atgataattc acccgatgta
     6901 gttattacta gtctgcttac tgatcattat aattctgggg cggtattttt agggacttca
     6961 cggggtgata ttatatggag taatgataaa ggtgaaactt ggcaggttgt ccagcgcttt
     7021 gatgatgtga taaaacgtat tgttatgagt cctaaggata gccgctttat gtttgctgct
     7081 actgctaata aagggttatt tagaacgagg gacggaggag cgacttggga aaatatcaat
     7141 caatctttag aagaatttca gattggcaac caatttcgtg atttagctat tactttggaa
     7201 gatcagccag tcacttattt agctactgat tatgggcttt ttaagtccgt taacttaggc
     7261 gatacttgga ctaaaattga attacttact cctgaaagtc agacagttat taacgcttta
     7321 gctattaatc ctcataatag tcaggagatt tattatgtga ctaatacgac tttttatcgt
     7381 tcactagacg gaggcaagag ttggtctact aagaaactac cgactaaacg agctggcaag
     7441 gtccttctag ttaatccaga gaagcccgag gttttatatc tagccgttca aaagattaag
     7501 aattaatatt tattaatgtt tactttaaag aagcaatcaa gtcagtcggg agctagactg
     7561 ggtcttttga caacagccca tggtaaagta gagactccag tttttatgac cgtggcgact
     7621 catggagtag tcaaacattt aactactgag gagttacggg aattggaggt gcccataatt
     7681 ttggccaata cttatcattt actgctaaat ccaggcattg agactcttaa acaagcagga
     7741 ggactgcatc attttatgaa ttgggaagga cccattttaa ccgatagtgg cggtttccag
     7801 gttttttctt taactggccg gcggatacat agtcacaatt tagttaaatt aaccgccgat
     7861 ggagtttatt ttaactcacc ccgtgatggc cggcgttatt atttaacccc agagctatct
     7921 ttagatatgc aggtagcctc taaagtagat attgcggtag ctttggatga gtgtttagcc
     7981 ttaccggcta gtttggctaa ggttcgtcag tcagttgagc ggactactgc ctgggccaag
     8041 cgagctaaag cccattatct tagcttgcct gagcctaaac cattactgtt tggagttatt
     8101 cagggtggag ttagtcgtaa gtggcgagaa cagagtcttt ctgaggtagt ggctttagat
     8161 tttgatggtt ataatattgg agggctatcg gttggcgaga cgactgcaga gatgtatgaa
     8221 gtactggaat ggttggagcc tcttttgcct cataacaaaa cacgttattt aatgggggta
     8281 ggttatcccg acaatattgt agaggcggtt agacggggag tagatatgtt tgattgtgtt
     8341 attcctacta gagaaggccg tcatggtcga ttattttttt ggcagcctga tcggcatttt
     8401 attgatgaga caggtaaggt gcgagaattt taccagataa aaaatattag aaatagctca
     8461 tttgctcaat tagatgagcc ggttaatcct tattctcgtt tgcctatttt acgtagttat
     8521 tcgttaagct atctacatta tttatataaa ataggagagc ctttaggctg tcgcctagct
     8581 agtcttaata atttggaatt ttattatgac cttatggcct taattagaga ggctattaag
     8641 aataatgaat tatagggata tggagataat gacagtatta actaggcagc ttcagccgcc
     8701 gcaagatgat tctcttgggg cccttataga aggattagat aaaattggcg gtcttaaaga
     8761 gggtgatgtt ttagctgtta cctctaaaat tgttagtatt tatgaaggac gagcgatagc
     8821 tatgtccagt atacaagata aggaccagct gattattagt caggctgagc gttatttacc
     8881 gcgagagatg gtaccgcata attatgctat tttgaccatt aaaaatcatg ttttagctcc
     8941 atcagcgggt attgatgaga gtaatggggc tgcttattat attttattgc cagctgaacc
     9001 agaaaacatt actagggcct ggcgtaatat tttactgcat cgttataatt tatctaatct
     9061 tggcttaatt ttaactgata gtcgttctac acctttacgg ctaggagtag tgggtattgg
     9121 tcttgctttt gctggttttc atccgcttag ggactatcgt ggtcagcctg atttatttgg
     9181 ccggcctatg aagatttctc aatctaatat tgttgattct ttagcggctg tggcggtata
     9241 tgctatgggg gagtgtgcag agcagacacc ggcagctatt attcgtggag tagatgggtt
     9301 acaatttact gacgatgatt gttcccaaga gttgtggcta gaccagagtg atgatattta
     9361 cgagccgtta tatcataatt ttttgccagg tggtggaatg aaataatatg ggaggggcaa
     9421 agaaacaaca gaagacacaa aagataattg ttgctctttc cgggggcgtg gattcagcag
     9481 ttgtcgccca actccttaaa gaggaaggac atgaagttat tggggtgtat ttagagttat
     9541 ggcatgatga ttattaccac acggcccatc aagaaagggg gaaagagcct tggtctgatg
     9601 cccaagctat ttgtcgcaag ataggcattc ctttttatgc tttaaattgt gctcaggagt
     9661 ttaagcgacg cgtagtcaat tattttttag cagcctataa ctcaggcctg actcccaatc
     9721 cttgcgtggt gtgtaataag caggttaaat ttaggcgtct tattcggtat gctcagcaga
     9781 ttggttttga tgctgtggct agcggtcact atgttcagct caaaggatat tcggggcact
     9841 atgagctatg gcgagcgaaa gataaggcta aagatcaatc ttatttttta tatcaactta
     9901 gtcaagggga cttagctcat cttcgttttc ctttgggaga actaactaag actgaggtac
     9961 gagctttagc ttatagttat catctgccag tagctagcaa gaaagatagt caggaggtgt
    10021 gttttataaa taatgacttg cctaatttct tgcagcgcta tttatccctt atacctggtc
    10081 cgattgttga cagtaatggt cgggtaattg gcaaacatga ggggttgccc ttatatacgc
    10141 ttggccagcg gcgaggcatt aaaattggtg gctcaggtcc ttattatgtc aagggttttg
    10201 actttttaac caatagtttg ctggttacta ataatgttaa tgaccctcaa ttatttagag
    10261 atactttttt tattccttat ttattttggt cttcaggtcg gcgaccacga cgagctattg
    10321 attgtcaagt agttattcgc taccatcatc aaccgctgga ttgtcagcta ataaaggaaa
    10381 ctcaaggagg atggcaagta gtgctagcta atccagagcg ctcgattact cctggtcaga
    10441 gtgcggtatt ttatgaggga gataaacttt taggcggtgg agttattgct ttacctgatt
    10501 ctttctgtta aactatagtc aacaaattat ggaatcattc taattttttg tatgttttta
    10561 gttttaaaac tatagtaatt ttaaatttat ggctacaaat accacttccc attttttaaa
    10621 acgagcctcg aaaatttctt ggccggaacg agtgattaat tgggtgttga tccttacttt
    10681 ttttattttg cccttatttt ttattaacat ttcctggcaa ggcgctaatc tagataaatt
    10741 attcttttta ggacttttag ttattattgg ttgggtggtt tggcttatta aagtattaaa
    10801 agagggagca ttgacttggc actggcgtaa attggattgg ctagttttag ctattttagt
    10861 tgttagtgcg gtagctactt tttggagccc ctcctggcac aatagttttt ttggcggcta
    10921 tggtcagcca atgcgcagtt taatattctt agtactcctt gttttattat attttctggc
    10981 ggttaataat tggtcggcta aattaaggcg gtcatcgtgg ttagcgattt taattagttt
    11041 tagttttatt accatttatt cgctttgcca acttttgggg ttttatatta ttcctttaag
    11101 ttttactaga actattagtt ttaatcctat tggctccctg tctaatttag ctttattttt
    11161 agccgcaact ttgccacttt ttattatagc tttaggtagt caagaagaat tttgtgagcg
    11221 gccatcatct aatggtaagc tatggtggaa tatctggttg attttgacta ttttagcgaa
    11281 tatagcagca atagtcatct tggggtcgtt taatatttgg ccggttgtta ttctgggttt
    11341 attaattgtt ttaatttttg ctatatctca ccttattaaa tttacccccc tacaattagg
    11401 gtttagtatt gggatgttaa ttattagttt tgttttgttg attgttggga atattggctg
    11461 gccgaaggta aatttaccga gtgaggtcag tttatctcgg ggcttttctt ggcagattgc
    11521 ccaggctagt ttggctaaac ggccgatttt tggtaatggt cttgctagtt ttgataatgc
    11581 ttttagccgt tttaagagtc cagactttaa tgctgcagcg ttatggaatc tggattttga
    11641 tgtacccacc ggctggttag cggaaagtct agtcattgtt ggtggtctag gctccctgct
    11701 tattgtcatt ggtttagtct gggctttagt tttggcagga cggcgtctct tgtcgtccaa
    11761 gactgagttg ccttctgctg atcttaaatt gggagctagt cttggggcgg cagtgatagt
    11821 tattataatt ggtggcttgt ttattccggt aggtaataat ttattattga ttttaggttt
    11881 gttgtggatt ttatttatgg ttgttactta taattggccc cggcgtcgct ctgttattta
    11941 ttggcgtaaa gctgatgagc gggcaaatgc cggctggatt gttggtttta ttgccgtagc
    12001 tatttttttg ctggctagtt tagcttatgg ggttaaaatt tatgcggccg atattttggc
    12061 ggctcaatct ataaagagcc agactactga ccagcaaata attactatgt ctcaagccta
    12121 taatttagct ccgtggcgag aaatgtatgg ttttggtttg gctcagttat cgtggattaa
    12181 agccaatgag ttagcgcaag ccgcaatgac ggcgacaggt actccggctc tggaagcgca
    12241 aactgctgct aagtcttatg ctcagcaagc taaagcaata ctagatcagg taagtaaatt
    12301 agtaaataat cagccagcta acttaaaaat ggcagcttca ctttatgaaa tgatcggtga
    12361 ttttgaagga gctatggcta ctcatcaacg tttaattgaa cttgatccta ataatccttg
    12421 gccctatgct aaattggctc agttaaaagt cgttcaggct tatcaagcgc aggataaaga
    12481 aactaaagat aaattagtcg aggaggcgct tagcggctat gctaaagcct tagaattaaa
    12541 gccggattgg gctgaggcct attattaccg ggcagtactt tatcaggcaa ttgagaagcc
    12601 ggtagaggcg acacaggatt tggttctagc agttaataat tctggtggag ctactaatta
    12661 tgttttgcct ttagcccaat tattaaatga acgagcgaaa atagaaactg attcggccgc
    12721 taatttacgg gctcaagctc agcaattagt agaaggtgta ctggccactg atgacagcaa
    12781 tgttaatgct ttgtatattt tagcgatgat ttaccgcgat tcaggccaaa ccgatttagc
    12841 ccgtgagaca gtcaacaaat tattagataa agctcaggag gcagataaac catttattga
    12901 acagcagttt gctgatttac tggctaaata atattttata atgactggcg gttggtcttc
    12961 cgttggctgt ttcttattgt aagatatgac tttaagttct caagagttac ggcaaaaatt
    13021 tttagatttt tttaggtcca agggtcatgc tattattcct agcgcttctc ttatcccgga
    13081 taatgatcct tcagtattgt ttaatactgc gggcatgcaa ccactggtgc cttatttatt
    13141 aggtgaacct catcctttgg gtcgccgttt agttaatgtt cagaaatgtt tgcggactga
    13201 tgatattgat aaagttggtg atacggtcca tttgactttt tttgagatgc tgggaaattg
    13261 gtcactgggt gattattttc aggaagaagc aattgcttgg agttgggagt ttttgactga
    13321 taagcagtgg ttagggttac cagctgatca tctagcggcg agtgtgtttg cgggagatgc
    13381 tgatgctcct tttgatcgcc gttcctatga gctctggcag caaatgggtt taccggagaa
    13441 gcgtttagct aaattgggcc aagaaaataa ttggtggggt cttgctagtc atgtagggcc
    13501 gtgcggtcca gatactgaga ttttttattg gactggccca gaacctgtgc ccgatatttt
    13561 tgaaccagct aatccatttt gggtagaaat atggaataac gtttttatgg cctataatga
    13621 acagccggat ggtactttta tccccttaaa gcagcgcaat gtagatactg gtttaggact
    13681 agaaagagta ttggcggtct taaatcatga gcctgatatt tatgtaagtg atttgtttca
    13741 gccagctatt cattatttag aaaaaataag tggtcgatta tatagtagtg aaactaggac
    13801 cttcagaatt attgctgacc atatgagagc cagcgctttt ctattagctg atgaaaaggc
    13861 agtaacacct agtaataaag accaaggtta tgtcctgcga cgcttgattc gtcgggctgt
    13921 ccgtttggtc tataattggt ctataccgcc ggcagatatt ttaagtggtg taattgaaca
    13981 aattattgct acaatgggtg aagcctatcc agagctggta agacaacatg attttattat
    14041 agctgaagtt aagaaggaaa ttattaagtt taattataca ttaggtcgtg gtctaaaaga
    14101 gtttgaaaga ttggttggtc accatcattt tattagcggg actgatgctt ttatgcttta
    14161 tcaaagctat ggttttcctt gggagatgac caaagaactc gctcaggagc ggggactagc
    14221 tgtcgatgaa gctggttttc aggaagaaat gaaaaaacat caagaattat cccggacggc
    14281 agcggctggc aaatttaaag gcggtctagc tgataattcc ctagccacta cgcggttgca
    14341 tacggcggcc catcttttac ttcaggcttt gcgacaggta ttgggagacg gggtttacca
    14401 gcgaggcagt aatattacgc ctgaacgtct acgctttgat ttttcttatc ctcaaaaatt
    14461 aactcctgaa gaaataaagc aagtagaaga tatagttaat gagcagatcg ctttggattt
    14521 accggttact tgtgaagaga tgtcactgga agaagcgcga cgggcaggcg ctatgggtgt
    14581 ttttgacgct aaatatgggg aaagagtgaa ggtatatacc attggcagtg atcggccttt
    14641 cagtcaggaa atttgcggtg gccctcatgt acagcgcact ggagaattag gtcatttccg
    14701 tattattaaa gaagaaagca gcagcgccgg ggtacggcgg attaaggcga ttttagaaga
    14761 ttaatatggt tcagcccact cttcttatta ctggtggagc tggttatatt ggttctcatg
    14821 cagttaagtt attcttagat aagggttata cggtggtggt tatggataat ttgtcgcggg
    14881 ggtggcagga gccgctagat attttagcct cccgaggtaa gttgattttt gttcgcgggg
    14941 acttaactgc atcatcagat ctagctactt tatttgagca ttatcatttt gctggggttt
    15001 tacattttgc tgctctatgt ctggtggatg aatcaatgtc caagcctgag ctttattttg
    15061 ataataatgt tattggcagt tttaatctat tggaagcgct gaggcgttat ggtcctgtgc
    15121 ctcttatttt ttcctccacc tgtgctgttt atggtgagag tcagtatctt ccagtagatg
    15181 aagctcatcc cttaaatcca gctaatccct atggggaatc taaattaatg gttgagaaaa
    15241 tgattaaatg gtatggagag cgctaccgga ttccttatgt aatcttaagg tattttaatg
    15301 tttccggagc agatgctgat ggtcttattg gtgatagtaa aaaacccagt gagttattaa
    15361 tgcagaatgc tgttcgtggt gctttaaaca ttgagccctt tacttatact tgtggtaaag
    15421 tagctactcc ggatggcaca ccaatccgtg attatattaa tgtagaagat ttaattgagg
    15481 ctcatgcttt agcttatgat tatcttaaaa atggcggcgt tagtgagatt tttaatttag
    15541 gtacaggcca gggttatagt gttaaagaaa ttgttcaggc ggtggaacat tattttggct
    15601 gtactttaaa aaagaaacaa ggagtagtgc gtcaaggtga atatgcgcaa atttatgctg
    15661 atattacgaa agccaaagaa gtactagggt ggcggccaag gcgaagccta gaagatagta
    15721 ttgttagttt agctaagtgg tatagtcgtt acccgcaggg gtataatttc taaatttact
    15781 cacactataa tgtttttaat ataattttgt attataataa ggatagtttg gttaacaatt
    15841 ttatttgccc ccccttatct tttgttataa tgttctcatg aggttattta ttatataata
    15901 ttagaatata taaatagttg agacaattca tgatgagtta ttcttaccaa cccaatatct
    15961 ttaagaaaca atcacattgt tatactaata atgcctttac tttagttgaa cttctcgtag
    16021 ttattgccat tattggtatt ctttctactt tatccgtagt agcttttaat aatgtgagag
    16081 ctaaagcccg agattctcgt cgtctctctg atgtaagaca aataggtatg ggtttagaaa
    16141 tgtattatga tgacaaagga gtatatcctg ccccacctac acctactggt actccaatta
    16201 ctggtttatg tcttagtaat ttaggtatta gttctacttg tggagcaatt gtctatcttc
    16261 aaaaaatccc tagtgatcct ttatctaata ttcattatac ctatagttat cttaatagtg
    16321 gagaaagtta ccgtctgggc tttaacctag aacaaggttc aggtgactgg ccagctggta
    16381 ccttagctat gggtcctaat ggtatcagtc aagacctcct ggcagccaat ggtattgact
    16441 ggcgtgaccc gaataattgg aaaaaaatcc caagctcagg cagtgggtgt accactacct
    16501 acgaccaagg caggaaagct ataaaaatag aaaatgagaa ccgatgtttt ctggctcctg
    16561 cctctgaccc tagctatttt cctattgata ccagccgtaa atactatatt gaagctgagt
    16621 atttaactga agggaccacc acatatggct tctatttggg gactgcatcg tataacgcaa
    16681 gttatattct cttgcctggt cataatggta cttatgatta ttttggaact tcctatgacc
    16741 gacccacttc aactaatacc tggactttta tcagcgccaa taaagccatt ggtggccgac
    16801 cacgcaccgg tacaagtact aacaggagcc ttctggataa atgggagcct ggcacggcct
    16861 tagccagagt actaatgata gctaattatg atactggcgc tcagactact tacatccgca
    16921 atattcgttt ttatgtagaa taggaaaaag attcatggct cagagtaaag ttattttaag
    16981 gtaaccttac tgtcagtttt ttaattttag ccctacttaa tcatagcaca ttgtggctat
    17041 tgtcctagaa acattaagta gatgatgata aaataataaa catgatagtt caaaataaac
    17101 accctctttc caatggtgaa gttatctggg atatagctgg tatgtctggg actggactga
    17161 tgctactatc ttaggtaaag acgaacctag gggcgtggct aatccttctg actttgactg
    17221 gtatgaatat accaaccttg tctcttatgg cactttttct ccggaacgtc ttttaccttt
    17281 aaatcttacc tggaatagta acaaaggtta tggccgaatt aagaccagcg gtgttccggg
    17341 cagtatgact aattatggct ttcttcgtgg tggatatcgt gagagtgata gttttgctgg
    17401 tgtattggca ttgaggttag atctcgattc acaatattcg gatagtgcaa ctggttttcg
    17461 ctgtgcacgg tagccaggta tcagcggcgt gatctctgcg cacttctttt atctaaaatc
    17521 tggagttttg acaatgagat atttgttttg tgcagaaaat gaggtgaact gatataggct
    17581 cttgacagac attatttttg cctttataat tagaccataa tttacaaatt agataatcat
    17641 aacatgataa tttatcagct ttggtttgat aaattatttt atgttaaaaa taattaatga
    17701 ccaaagctaa cagtaccacc aatgattcca agagtttctt agagtgtgag ggggaaatct
    17761 tagaattaat gcctggttta acttttaagg tccgattgga taatggccac gaattattag
    17821 cccatttagc gggtaagatg aggattaata aaattaggct agcaccgggt gatcgggttc
    17881 gcctagaaat tagtccctat gatttgacta agggtcggat tatctatcgt ctttagggta
    17941 aagcaattat gaaagtgaga gcttctgtaa aaaaaatgtg taaagattgt aaaattattc
    18001 gtcgctttaa tcgggtgcgg gtaatctgta aaaacccaaa gcataaacag cgtcagggtt
    18061 agtttaacta tattttatga cagtaagaat tgctggcgtt gttttgccta atgataagcg
    18121 aattgaagtt gccctaacct atatttacgg tttgggtatt tctaaatcac ggcagatttt
    18181 ggctaaagct aaggtcaatc ccgatacccg gactaaggat ctaaccgaag atgaagctaa
    18241 taggcttagg attttaattg aaaaaaatga agtagtagaa ggtgatttac ggcgagaagt
    18301 aagtagcaat attaaacgtc ttaaagatat tgggtgttat cgagggatac gccacagccg
    18361 taatttgcca gtacgtggcc agcgtaccaa aaccaatagt cgtactgtgc gcggtaatcg
    18421 tcgtcagaca atgggttcag gacgtaagcc accagctcag aagacctaat ttaattattg
    18481 taatatggct gatgaacaat caacaaaaga acaagaggta accaaagaag caactgagga
    18541 tttagagaat ctagctgcaa gtgagtcaat ggctgaattg cctgaagaag ttaagaagaa
    18601 gttagagaag actaagtcta gtcggttatc ggtagctaag ggtaaacatc gccgtaaggt
    18661 ggagcggacc ttaactaagg gtaatgttta tattaaatct acctataata atactattgt
    18721 tactattact gatgagaatg gtgatgtagt tacttgggcc agcgctggac tggcaggatt
    18781 taggggccct aaaaaatcta ccccttatgc ggctcaggtt attaccaaga ttgctgtaga
    18841 tcgagctaaa gaaatgggat taactgatgt acaggtgttt gtcagcggag tggcggcagg
    18901 ccgagaagct gcgattcggg ctttaaatgc taatggttta atggttaatt cgattaaaga
    18961 tgttacccca ataccacata atggttgccg tccgcgtaaa ccgcgacgtg tttaattaga
    19021 aaattgtcta tgtctagata tttaggatca gcttgtaaat tgtgtcgtcg tgaaggcaca
    19081 aaattaatgt taaaagggga acgttgcagc tccagcaagt gtgctttatc tcgacgtaat
    19141 tttccgcctg gcttccacgg gccgcaaggt cggccgcgtc aatctgaata tagtctacag
    19201 ttgcgggaaa aacagaaagc taagcggatg tatggtttgc tagaaaagca gttttattta
    19261 acttttctta atgctaaaaa acaaccaggg gatgcgggtg aaaatttatt gcgtctttta
    19321 gaaatgcgtt tagataatgt agtgtatcga gctgggtttg ctgattctcg agctaaggca
    19381 cgggaattag tgagtcatgc tcattttact gttaatggtc atccagttga cattccttcc
    19441 tatcgggtta agccaggtga tattattaaa gtgaaatcca gccggcgtcg taaccagatt
    19501 tttaatggat taggagaacg tttgttgaaa aagaaatttc aagcgcctag ttggttattt
    19561 ttagatccta gcgagttaga ggttaaggtt cttcatgagc ctaaggagga ggatttagag
    19621 aaaaatatta atactcaact cattgttgag ttttacgctc gttaatcttt atttaaattt
    19681 tatggaaaaa attgctttgc cacaaaaaat ttctgttttg ccagggaatc atcctaatga
    19741 ggctttaatt actgttgagc cgctttttcc tggctatgga acaacagtgg gcaatgcttt
    19801 acggcgagtg ttattatcct ctttaccggg agcggcagtg attggagtaa agattgctgg
    19861 tgcggatcat gaatttatgg gattgcccgg tatagctgaa gatgttctta ctattatttt
    19921 aaatcttaaa aaattacgtc taaggatttt tagtgaagaa atttgcaaat taagtttaga
    19981 ggccagtggg caaaaagttg tgacagccgc tgatattact cctaatagtc aggtggaaat
    20041 tattaaccca gacttagtat tggctcattt aactagtaaa gacgctaatt taagtatgga
    20101 gatttttgtc agtcgtggcc ggggttatga gatggtggaa aataggccta aagttaaaag
    20161 ggaaattggt tatattgaaa ttgattctat ttatactcca gtggtggccg cgggattatc
    20221 cgtagagaat gtacgggtag gccagatggt gaattgggat aaattagttt taagaattgt
    20281 gaccgatggt actatgacac cgcgtcaggc ctttattgat gccagtaagg ttctaattga
    20341 tcaatttact gctatcgttg atttggcaga agttgagcca gaattagaag gggaattagc
    20401 cgttaatggt gagaagaaac ccagtaaggt taaagtaagt gctaaaaaat ctaaaaaagg
    20461 tacgacttct aagagtaaaa taagtaaagc caagtaatat gcgtcataga aataaaggag
    20521 ttattttaga tcgagccaaa gaaccgcggg agatgatgct ccgtaattta gcggcgagta
    20581 ttttaatgca tgaatttgtt gtcactacta aagccaaggc taaggcagtg cagccattgg
    20641 tagaacatgt attaactatt gctaaggctg gtgatttagc agcgcgccgg cgtcttatag
    20701 ctattttgcc ccaagataaa gcagtatcta aggctctaga attagctaaa cgatacgaat
    20761 ccagaaatgg cggctacact aggattattt ctttaggatg ccgtcagggt gatggagctc
    20821 agcaggtgcg tctagaatta gtttaatttt tatttatggt acaaagagag atttatacta
    20881 ttgatgcctc aggtaaaact attggtcgtc tggctagcca gattgctctt ttactgcgag
    20941 gtaaaaataa agccagctat aagccccagg tagatgaggg cggagtagtt tatgtgaaaa
    21001 atatccgtga ggttgtgttt actggcaaaa aattagagca gaaaaaatat taccgccata
    21061 gcggttatcc ggggggccta aagagtaagg ccatgtctca agtacaagct gagcagcctg
    21121 atgaagtctt aagacgggcg gttaaacaaa tgttgccagc taatcgcttg cgcgaagcta
    21181 tgcttaaacg tctgataatt aattagttat attatggtgg atgaattaga tgagttagaa
    21241 tttgccatta gccaagataa atcatccgtt cctttagagg aagcgatgga ttatagtaca
    21301 acccgagagt ttactggtaa gtatctaaga gcggtaggta aacgtaagac ggctagtgcc
    21361 caggtgcgcc tttatgataa aggtagtggt attattgtag ttaatggtca ggccttagct
    21421 agctattttc cagtatggtc agatcaggcc gctgttatcc aaccattgcg gctaagtggc
    21481 ttacagaaaa atttggattt ttccattgtt gtaaggggtg gtggtaagac tggtcaggcc
    21541 caggccatag ctcatggcat tgctttatgt ttgctcaagc ttaatccaga tttaaggtca
    21601 gtattgaggg ctaaaggttt ggttacccgt gatgcccgga ttaaagaacg gaagaagcca
    21661 ggactaaaac gggcgcgtcg tgctccgcaa tggtccaaac gttaatttgg tgttttcttt
    21721 gtactttacg atattagcaa aaatgccagc tcaggctggt atttttgttt ggattattgt
    21781 cataatttat tgctcctcat ccttaaatgt tgtatagtaa gtcatagtga ttctatttat
    21841 gccagctcga gttactaata ttgctaaaaa tacttcttat tttactttgg ccctggttat
    21901 ccagaagcta atttctttaa catactttac tctttacgcg cgggtactag ggccggctga
    21961 tttgggtaag tattatctag ctatttcttt aactagtatt tttgctattt ttattgatat
    22021 tggtttatct aatgtattaa cgagagaagt agccaaatac ccggataagg ctagtaattg
    22081 gctgggcaca gttttggcta ttaaaattcc tttagccatt attaccaccc tggcagttgt
    22141 tttgtttgtg aacattttag gctatcctct attgactaag gaattggttt atatttctat
    22201 catttccatg gtgctagact cctttactgt gacctttttt gctgtgtcac ggggctttca
    22261 taatcttaaa tttgaaagtg tttcttcagt gattgttcag agtattatct tagtagttag
    22321 tttaatggtt tttcattatg ggggtggggt gcgctggctt atgttctcgc tggtcttggc
    22381 tagcagtttt aatttttgtt attctttatt tgttttatgg cgctattggc gtcttccggt
    22441 tcggcccctg tggcgtcgga catggattaa aaaagcaatt ttattagcaa caccttttgc
    22501 cggttatgga atttttcagc gcgcctatat ttatttagat acagttctgc tttctaaaat
    22561 atctggtgat gtggctactg ggatttacca aattcctttt aaaattatta atgctttgca
    22621 gtttttgcct atggccttca ttgcttctct ttatccggcc ctaagccttt attggcagga
    22681 aaatcgggag caattatcta ttacttttaa tcgggcgatg cattattcag ctattatttc
    22741 tttgcctctt actattggcg gttgggtagt agctgagccg cttgtagcta tttttagttc
    22801 tagctatacg ggggcggtat ggccgcttcg cctttgtctt atcgccttgc ctttcatgtt
    22861 tttaggctat cctattggtt ctcttcttaa tgcttgtgat gagcaacggg ctaatacccg
    22921 caatatgttc attgttctta ttactagtgt agtcatgaat ctattattaa ttcccccctt
    22981 aggggcggta ggggccagta ttaccgtggt tattactaat ggtttattgg ttcttttggg
    23041 tttaattagt gccaagaaga ttgctcctca tttatggcgc tggcttaagc aatttggcca
    23101 gactattctt gcctctttaa ttatgggggt aatagtttgg ttgttgaggc catattgtaa
    23161 tgtttttgta gttattatta ttggcgcttt agtttatggc ttagctttgc tgcttactgg
    23221 ttcacttact aaagctgatg tgcaaagtgt tattcagtca tttcgccgtc ggccgactaa
    23281 ttaatattta tgcgctgtct tttagttact cttgaatatc caccatttta tggaggtatt
    23341 gctaactatt atggtcatct agttaaggcc tggcctcagg cagatgaagt gacagtgtta
    23401 gataataata aaggacaatt gttagcccct caagggaagt ggccgtggcg tcggtcgtta
    23461 gttagtctgt ggcgtgcctt aaaacagggt cattatgatt ttgttttagt gggtcaaatt
    23521 ttgccgttag gcacggttgt ctggttgttg tcttttattt ttcctattaa atatggggtc
    23581 tttcttcatg ggatggactg gggtgaagct ttaaagcggc ctcgtaaatc atggttagcg
    23641 caacgtattt taagacgttc cgctttaatt attgcggcta ataattatac ggctgactta
    23701 gtaaaaggta ctatgccttt attaagtagt cgtatcattg ttgttaatcc aggaatagat
    23761 agtgttgagc cccccttggt tgctcctgaa tatttagcca gtttgcggca gcgttataac
    23821 ttagagggtt gtcagatact ccttacggtt ggccgggtag tggagcgcaa gggagtagat
    23881 aaagtgctgg aagccttgcc tcaattattg ccatattacc ctaagttgcg ctatattata
    23941 ttaggtaatg gtccttattt accagtttgc cagcagttaa tggctgattt ggggattaag
    24001 gatgaggtca tatttattac taatgccact gatgaggaaa aagcagcgtg gtaccagtta
    24061 gccgatatct ttattatgcc ggcccgggct aaagatggtg attttgaagg ttttggcatt
    24121 gtttatttag aggctaacca ggctggtaag ccagtaattg ccggtcaaag tggtggggta
    24181 ggggatgcgg tggttgatgg agtaactggc ttaatggttg atccgcaatc acctagggcc
    24241 atagctcaag cggtccgccg cctacttaat gatgaatcat taaggcggca gttggggaaa
    24301 aatggacagg cccgtctgca gcaattttta tggccccgcc aggctcatct tttaagagat
    24361 aaaattaaag aagttttatg atctcggtta ttattcctgt atataatcag gcagataaaa
    24421 ttggtcgttg tcttgatagt cttttagcgc agacttatcg tgattttgag attattattg
    24481 ttgatgatgg ctcaacagat aatttagatg aggttctctt agcctatgaa actaagttac
    24541 aggacgccaa gattccttat aaaattattg tgcaagaaaa tcggggagct aatgctgctc
    24601 ggaatcgggg ctgggccgag gctcaaggcg attttcttct ctttagtgat gctgatatta
    24661 cctggcggcc tgatgcttta gaaaaattag tcaatagttt aattcataat cctcaagcta
    24721 gttattgtta ttcctctttt cgtttaggtc gaaagaagtt tcatttatgg cctttcgatc
    24781 cagaacggtt atatcgtgaa ccttatattc atacttccgc tttaattcga cgcgctgatt
    24841 ttcccggttt tgacaatact cttaaacgtt tccaggattg ggatttgtgg ctaactatgc
    24901 tcgagcaagg taaaaagggt ttattttatg atgatttttt atttacagtt gccagtggag
    24961 ggcgaataag taaatggctg cctagttttg cctacaagat ttttcctttt ctacctaagg
    25021 ttaaaaagta tcaggaagca gcagccgtta ttaaagccaa acatcattta ccctatgaaa
    25081 ccaaatagac acctgtggtg gctaatcata attatcctaa taattattgg catcattgtt
    25141 tatggattat ggcgggctaa ccatcgtggt cctcgtttta tggctgatag tgaattagta
    25201 ttgcctgatg gtagccaggt tcagattgct cttttaacta ctaaagctga aaaataccgc
    25261 ggttttagtg gaatgcgtca gccttgtccg gactgtggtt tattatttgt ctggccggcg
    25321 ctatatcagc cggtaatagt aatgagagat atgcttttcc cccttgattt agtatggcta
    25381 agagataagc agattgtcca gttgacttta aatattcagc cagaagaagg agaggagtca
    25441 gagctgacga aatatgaacc tagtgagccg atagatgcag ttttggaggt gccagctggt
    25501 tttgctggac gtcataattt aaaaattggc caatttgtag attggcatta atggtaaata
    25561 tatgttgaaa tcagtgagtt ggactagggt gcgttacatt ttggaagcta ttatcatagt
    25621 tgaattatta tctgccttaa cttatttttg tccaactatt ggaccctggg tttttattct
    25681 tgctatgctg gccggtcttt atttagccat agtaagacta gattgggcta tttatgcttt
    25741 gcttattgat ctcatgattg attctaaggg ttatttgttg tcgctttctc tgggtggctt
    25801 tcgcttatct tggcgtattg gtctctggct aattattatg gtggtatggc ttattaaaga
    25861 attaatagct ctaagacagc atcgtacatc attagcttta gagtggcata aatttaaatc
    25921 cagcgggatg gttatttttt tactaatgtt ggtgtggggt ataattattg gtttaattcg
    25981 aggcaatagt ttaagcaatc tcttttttga tgctaatggt tggttattcg tattacttat
    26041 tgggccacta ctttctatta aaaaatggcc gtctcaagag tggtcggcgg tcctcactgc
    26101 tggtatggcc tggcttatta ctaaaactag tttactgcta tatgttttta ctcatttatc
    26161 tactatatgg caagcaataa tttaccgctg ggttagggat actcaggtgg gggaaattac
    26221 caggatgtca gctcaatttt atcggatttt tttgcagtcc catatttatt tattaatttt
    26281 ggtggttatt gccagttggt tgttggctac taaattgcct cgcactcagt gggagaagtg
    26341 gttggtcaag gataagggtt attggtggat tattgggttg ggtttagctt taatgcctat
    26401 tattgttggc tctgcccgta ctttttggct agctttaggg gtcctaatgc tctttatctt
    26461 tatacttatt gctcgtcgtt atcattggtc tatcgcttgg cgttggctta taaccatgat
    26521 tttggccggc tttgttgctc ttataagtat ttttattatt gtgagtttcc cttggcctaa
    26581 gcctcaggta tcttttaacc cgggtgatat tgtggaagaa cgattggggg atttagcaaa
    26641 tgaagctgga gcttcttcgc gttggtccct cctgccagct atgcttcagg gtattagcaa
    26701 ggcaccactg ctggggcatg gctttggagc aactattacc tatatctctg ccgatccgcg
    26761 cgttctggct caaaatccta gtggttctta tactacttat gcttttgaat ggggctggtt
    26821 agatatagct tttaagtttg gcttaatttt tgctttgtgg tatttggttt ggcttatctg
    26881 gttaattagt cgggcctgga ggcatggttt aacaggagaa atctgggcct taggtttagg
    26941 tactttggta gtagtccact tttttagtcc ttatcttaat catcctttag gaataatgtg
    27001 gctagttggg ctaatagctt ggctctcgag ccataaacaa tatgaatcat tatcttctag
    27061 ctagcttatt agcttttatt ttgtcggctg ctttaactcc gcttattaag caattggcat
    27121 ggcattggca tattgttgac cggcctgatc aagtgcgtaa attacagact aaaccagtag
    27181 ctctcttggg ggggtgggct atttttatcg ccgctagtct agtaatgtgg ctttttcgtg
    27241 attatcttat cgccggtgat ttaactggcc gtcattggct cggagtctgg attggtggtt
    27301 taattttaat gattggcggg actctagatg acctttataa tttacgacct agcgcacaat
    27361 tagttttttc tattttggct gtgctagcgg ttattattgg gggggtgggg attaataagg
    27421 taacgggtct taatggagag ttaatatttt taaataaatg gcagattagt ttatttactt
    27481 ggcaaggcat tactcgtcat tttgtggttc tagctgacct ctttacttta acttggctga
    27541 tggccttaat gtatactact aaattattag atgggctaga cgggttggtt actggcctta
    27601 ttggcattgg tagtattatt attgctttat ttactttaac tactaaatat taccagcctg
    27661 acattgcctt agctagctta atttttgccg cggcctgtgc tggttttttg ttatataatt
    27721 ggtcaccggc caaaatttat ttaggtgatg ggggttcact gcttttgggt tattttttgg
    27781 gagtattagc cattatctcg ggaggcaaaa ttgcgatcgc tttattaatt atgggaattc
    27841 cgatgcttga tgttgcttgg gtaatagttc gtcgtctctt aaatggtgaa aatcccttca
    27901 aatcagctga ccgggggcat ttacattacc ggctattaga cttggggtgg ggggtacggc
    27961 gctcggtctt ggcttattat cttatagctt tagtctttgg agttagtgct ttatttttac
    28021 aaagtcgggg aaaacgttta gctttacttc ttttattttt agtgatggcc attgtcgttg
    28081 tttatttttc tcgacttgac aaggctagaa aaagaccgta atataaaggg atatcttaaa
    28141 cagggaagtt tttaatacta tagatcatta tatgtcaaaa ttagcaataa tattaagcgg
    28201 tggcaagcag tataaagtaa agccaggaca aattatcaag gtagaaaaat tagcttctga
    28261 gccaggaacc gaggttacct tagagacttt acttttagct gatgagacca gcggagaggt
    28321 gcaagtaggt cggccatttt tagaaaatgg agtgaaggct aaggttattg aacatagtcg
    28381 cgcaccaaag gtaagggtgg ttaaatacaa aaataagact cgctacaaga agaccactgg
    28441 ccaccgtcag ccttttacta agattgaaat tgctgagtgg taagacaaaa caacctattt
    28501 ttataggttg ttttttatct taagctcttg acttatctcc ataaagatgt taaatttcta
    28561 gtgcgtttta tctaaaaaat atgtctatga ctagccagac taatcaatct gctgattcta
    28621 tttttacttc taatgatttt tctttattta aattagtcaa caaattatta caaaaattaa
    28681 atccccgaga acgggaagtt ttaaggcgtc gttttggttt aaataatccg cataaagaga
    28741 cattagaaca catcgggtct acctatggtt tgactcgaga aagggtacga caaattgagg
    28801 cggctggagt taagaaatta cagaaattac gggatatgga gaaacagctt attttactgc
    28861 gggatgccct agatgacctt ttagctgata atggcggttt gatggataaa gaatatttag
    28921 aaagtagctt tttagtttta agccatgaaa gtttacaaga atctcaacgg ccgatttatc
    28981 gtaataattt tgatttctta gtttctaagg tactagccga tgattttgat ccagtaactt
    29041 ctgatttatt taatgattca gtaaaattaa agtatcaggc tattgatcat ttagaaaaat
    29101 tggcccagga actagtagag caggtgcgtc aaaccaggcg cctttttcta ttggaagatt
    29161 taattaaatt aagttttgag ctagatgctt ttaaggaaaa tgctgataaa ttagtaagca
    29221 ctaataagtt tgatttagct agtgctttgg ctggttatga aaattttcaa gatggtgagc
    29281 taattaataa atataaacca atttattcgg tattgcgggc tacccggcat ttagaagaaa
    29341 ataaatttgg agtctgggga gaagccgatt tgccagaaat tagaccgcga actgttgcag
    29401 ataaaattta tttagttctt aaagatgctg gtcagccctt acattttcgg gagattaccg
    29461 agcgtattaa tcgtgttttt acagataagc ccgctaattc cgctacggtt cacaacgaat
    29521 taattcttga tgaccgttat gtgctggtgg ggcgaggtat atatgccctt aaagaatcag
    29581 gctatcaggc aggaagagta gtagatattg ttagtcgtgt tttacgccaa gctcgccgtc
    29641 ctcttagtaa agaagaagtc attaagaaag tattagagca aagattagtt aaaccggcaa
    29701 cgattaattt tattcttgct aataaggaat tattccagaa actgcctgat ggcacttata
    29761 ccttaaaaga atcagctgtt tagcttgctt ttattttgtt agggcttgtg ataaactgga
    29821 gttaatttgt tagttagtaa ttatgaattt aacaccgtta ataaattttt tctatttgcc
    29881 agctgatgaa atagtttggc ggttggtttt ctattatggc tggatgattc cggccataat
    29941 gtttttaaag ggttcactta tggtctggct tagttggcgt cgtcgtttat tttttagtaa
    30001 gcagcacttt actttattag ctatagatat tcctcgtggt aatgagcaaa cccccaaggc
    30061 cctagaaaat atgctggctt atttggccgg agcccatagc aatcctaatc tcatagaaac
    30121 ttattgggaa ggtaagtgtc agcttggttt tagtttagaa gtagtaggta ttgatggcta
    30181 tattcaattt ttggtccgta cacccgattc atttcgtaat ctggtagaga cggcaatcta
    30241 tgctcaatat cctgatgcag agattgctga ggtagaagat tatgttgata cagtgccgga
    30301 caattttccc aatccagatt ttgatatgtg gggggctgaa tttgtgccag tggcaatgaa
    30361 tccaccatat aagcaagctt tgccgattaa aacttataaa gattttgaat acgactatgg
    30421 tcggccagaa tatcacttta aagatccaat ggctagtttg atggacctta tgagttctct
    30481 taagccgggc gaacaaatat ggtatcaaat attgttaata ccaactgtgg ccgattggcc
    30541 ggctcttggc caagaggcta ttaataatat attaggcaat aaacctaagt ctaaagagac
    30601 tatcattgaa aaattttttg atgggatact taatttgatt agccaaattg gtgattttgt
    30661 ttttgatggg atacttaatt tgattagcca aattggtgat tttgttggtg gtatttttgg
    30721 cttgggaggt actagcagcc aaacttcaga taaaaaagag gaaaaagaag atcaatttaa
    30781 gatgatgaac ttgttgccaa ttcagaaaaa acaaatagaa gctattcagg ataaggtatc
    30841 taaaataggt tatctggtga aaattagaat gatttatgtg gctagacgtg aggttatgaa
    30901 taaggctaaa gcagtaaatg gctttgtggg ttttattaag cagtttaata ccagcgacct
    30961 taatagttta aaaccagata gtaaggttac tcaaacctca gcggattatt ttaatagatt
    31021 aaatattatt aatagcagga aaaataaaat tatgcaggct tataaagctc gtgatgatag
    31081 tgctggtcgg tcgccttggt atttaagtgt agaagagatt gcgacgttgt ggcattttcc
    31141 gatagaaagt gtagtgaagg ctccgttatt acaaaaggct agcagtaaaa aaatggagcc
    31201 gccaatggct ttacccactg aggagactaa tgaggaagaa attgaacttg accaattatt
    31261 tactttagat tcagaagatg aattaacccc ggctgagcca agtgaaaatt ctaagtccaa
    31321 agaagttaat acggacgaga gagataatac ttttcatcag gccccaccgc ctagtaattt
    31381 acctggtatt taataatgat tttttatttt tatggatgaa gatgttactt tatttggcga
    31441 aactactttt cgcaatcaaa atcataagtt tggtatcaaa cttgatgatc gccggcgaca
    31501 tatgtatgtt atcggtaaat caggtatggg taaatcaact cttttagaga atatgattgc
    31561 ccaggatatt aataaggggc gaggattagc cgtagttgac ccgcatggtg atttagcaga
    31621 gaaaattatt cattatattc ctgataaacg catagaagag gtagtatatt ttaatccagc
    31681 tgatgccgaa taccccattg cttttaatgt cgtagaacaa gttgaccctc gtttacgtca
    31741 tttagtagcc tccggcctta ttggtgtttt tcagaaatta tgggctgatt cttgggggcc
    31801 acgtttggaa tatattttgc gtaatgccat tctggcggta ttagattatc ctgattctac
    31861 tttgttggcc attactcgga tgctatcgga taaaaatttt cgtaagaggg tagtggagaa
    31921 aatccaagac ccggtagtta aagccttttg ggttaacgaa tttagcagct ataatgataa
    31981 atttgcttca gaagcagtag ctcctattca gaataaggtt ggccaatttt tatctagctc
    32041 cttaattcgt aatattgtag ggcaggtaag gtcatcgatt gaccttaggg acattatgga
    32101 taatggtaaa attttgatta tgaatttaag taaaggacgt attggtgaag ataattcagc
    32161 cttacttggt tcaatgatga ttactaaact tcaattagcc gcgatgagtc gagtagatac
    32221 accagaagaa gaacggcgcg acttttttct ttatgttgat gaatttcaaa attttgctac
    32281 tgatagcttt gctaatattc tctccgaagc ccgtaaatac catctaagtt taatattagc
    32341 tcatcaatat attgaccagt tgacggatag tgtccgggct gctgtatttg gaaatgtggg
    32401 cactttaata acttttcggg taggggcgac tgatgccgaa tttttagcta aagagttttt
    32461 gcctttattt actgaagaag atttagttaa tatccccaaa tttaattttt atcttaaatt
    32521 aatgattgat ggcataacct ctgacccatt ttctgctaga accttgccac cattaagtaa
    32581 ggatgagcaa actaataatg tggataaagt cattgaatat actcgtagcc attatgctaa
    32641 tcgtcgagat atagtggagg aagaaattat gcgttggcat cttaatcagg gaggggttgc
    32701 agaaagagaa aatagggtgg ttaatgtttt agaacaacct gaagttctaa actcaactac
    32761 taaaatggat gttttagagc agcgttttcc tgccgtttgt gatggctgtg ggcagccgac
    32821 cactgttcct tttaagccgg atggccagcg accagtatat tgccctgatt gtctaaagcg
    32881 ctatcgggca gaacggagtg agcgccaacg tttattacaa tcttccccta gccagttaga
    32941 tcacttagag ggtgcgtctt taagacaaat tcaccgacct aacattagta aaaataataa
    33001 caaaggaaat aatggacaaa gtagccattg attatttaaa aattggtcga gcatctgaac
    33061 ttactggtcg agaccgtagg ctttatcgtt ttttagaaat tctgccgggg ttgctatcgt
    33121 ggctaacttt gctagtatta attatttttt cttattttca gcccgtctgg gtggctatat
    33181 ttgttattgc ttttgatgtt tattggctat tattggttgt ctatttagcc cttattttaa
    33241 tttcagctta tcgtcagatc actcataatt taaaggttga ttggcgggcc cagtgtctag
    33301 ctttaccgcc agtagattta gtttatagac ctctacaggg tgagccaata gtaagttcgg
    33361 gggtgaggtg gaatgattta tatcatttgg tgatttttcc ttgttataat gaaggttata
    33421 atatcattca gccaagtatt gaagctttag ttaagtcacc ttttcctcga gataaaatga
    33481 tagtcatttt agcaattgaa gagcggggtg ggccagctat tcaggctgta gccgaaaggc
    33541 tgcgtcaaga atatgagaat agttttttta agtttagggt tattgtccat cctgataatt
    33601 tagccgggga aattaaaggc aagggagcta atcaagcctg ggcggctaag aaggctaaag
    33661 aagaaattat tgatcctttg ggcatagact ataatttaat tcttgccagt gtttttgata
    33721 gtgatacagt tatttatgaa aattatttct attgtttaag ctataaattt ctaaccacca
    33781 aaaatcccta tcgtcgtagc tatcagccga tcccgatgta tcataataat atttggcaag
    33841 caccattttt tgcccgggtg gctgcttatt ccaatacttt ttggcaaatg atgcaacaga
    33901 ttcgccaaga gaaattagct acttattctt ctcattcaat gccgtggcgg gctttggtag
    33961 aaattggttt ttggtctact aaaatggtca gtgaagattc gagaattttt tggcattgct
    34021 tttgttatta ccggggtgat tatgaggttg aaccgcttta ttatccagta tctatggatg
    34081 tttgtatgga tgagactgtt tggcagacgg ctcgcaattt atataagcag caacgacgct
    34141 ggggttgggg ggtggaaaac gtaccctact taatatttaa tactattaag gagtggcatg
    34201 ttattccgaa aaaagttttt ttggataaaa tttttattca gttctatggt tttcattctt
    34261 gggctactaa tgctataatt attgggtgta ttggttggtt acccttattt ttaggagggg
    34321 atcgttttaa ccagacagtt ttatctaata atttgcctaa tgttaccagg atattaatga
    34381 cgatcgcgat ggttggcatg gtgctatcag ccattatttc ttctttactt ttaccgccac
    34441 ggccagccaa gacgccgtgg tggcgtaaat tagggatgat agctcaatgg atttttttgc
    34501 cagtcactat tgtagtcttt ggggctattc ctggcctaga agcgcaaact agattaatgt
    34561 tgggcaaata tatgggattt tgggtaactc ctaaagcaag aagtggttaa attaattttt
    34621 aataatatgc cttttattga tccagatcgt ccagggacta cctttttaag aatttttgct
    34681 gttgaatttg cagccattat tgtagtctat ggtattttag ctttattata attatgctga
    34741 agttgcggct ccataatact ttatcgcggc agttggaggt ttttaagcca caacagccgc
    34801 cagttgttgg tttatatacc tgcggtccga ctgtctataa ctatgcccac ttaggtaatc
    34861 tgcgggctta tgttttttct gatctactga agcgcacctt gttatataat ggttatgaag
    34921 ttaaacatat aatgaatatc accgatgtgg gacatttgac tgacgatgcc gatgctgggg
    34981 aagataaagt ggagataagg gcagagcgag aaggtaaaag tgcgtgggag attgctgatt
    35041 tttatattaa ggcttttaag gatgaccttc aggctttaaa tattataccg ccgactactt
    35101 attgccgggc taccgaatat attcaagaac aaattgagct agcgcagatt ctagaacaaa
    35161 aaggttacct ttatcgtatt agcgatgggc tttattttga tactagtcta gtacctgatt
    35221 ataataaatt gagtcattta cccctagaga aattaaaaga aggggcacga atagataaaa
    35281 atcccgagaa acgccatccc actgattttg cagtgtggaa atttagtcca ttgggacaaa
    35341 agaggcaaat ggaatgggat tcaccctggg gtaagggttt tccgggctgg cacttagagt
    35401 gtgccgctat gagtttaaaa ctattagctg gccatttaga tattcatacg ggcggagtag
    35461 atcatattaa tgttcaccat accaatgaaa ttgctcaagc tgaagctgcg acgggcaagc
    35521 catttttcca ttattggctt cataatgctt ttttaaatat tgccggcggt aaaaaaatgg
    35581 ctaaaagcgg agctaatttt ttgactttaa aggtggcggt gattgatgag ggatggttgc
    35641 cgttggctta tcggtatgcc tgtttagagg tacattaccg caagcccatg gaatatagta
    35701 aggctagttt agctgccgcg gatcaagccc tgaaaggatt atatcgtgaa gttgcagctt
    35761 taggtgggga gatgggtgaa gtatcgccct tgttcagaga gaaatttgta gaagcaatta
    35821 atcatgacct aaatagtccc caagccctag cagtgctttt tgaagtttta aggagtcagt
    35881 tatctaataa agataaacta gccaccatca aaaactttga tgagattttt ggcttagatc
    35941 ttattggggg cggacaacat tattcagaga tgcttactaa tataccgcca gccgtacagg
    36001 cattatttaa caggcgacag ctagcacggg aggcccagga ttttccatta gctgatgaat
    36061 tgcgacgtca attagctgaa gctggttatg gcgtagagga tgggccaggg ggtagtcgac
    36121 tcacgccttt attttaaata tggataattt ctgggctcat ttagctcggc cgtttactgt
    36181 gttggccccg cttagtggtt ttactgatag ggcttttcgg cagatttgtc gtagctatgg
    36241 ggccgatgtg gtagttagtg aaatggccag tgtcgctgcc ctagttcata gtccggagaa
    36301 agctctagaa agattatatt ttaatgagat tgaaagacct tacgtcgttc aattatttgg
    36361 tgctgaccct aatcaatttg gagaagcagc ccggctggta actaatagaa ttcagcctga
    36421 tggcttagat attaattgtg gctgtccagc tccgaaaatt attaaagaag gagcaggggc
    36481 cgctttggga gctgatttgc cccgggcacg gcaggcagtt caggcggtat taagtaatac
    36541 tagcttgccg gtatctataa aactgcgagc tcagaataag gatattagcg ctcagcaagt
    36601 ggtagcttat ttagccgatt tacctttagc ggccgtgacg gttcatggcc gtagtgttag
    36661 tggaggcttt agcggaccag tagatccagg ggctattgct ctttgccgtc cctatgtcgc
    36721 cggagtttta atagccaatg gtgggatagt tgatttaatg actgcacaga taatgctcca
    36781 gaaaagtttg gctgatggta ttatgattgg tcgtggggca ttgggccgac cttggatttt
    36841 tcaggaaatt aaagagggac gagaaattaa cttaacttgg actgaattga cagaattaat
    36901 aaggcatcac gctcagctta tagtagctta taaaggagaa gttggtctta ttgaatttcg
    36961 taaattttta gcctggtatg ttaagggttg gccgcaagct aaagcattac gtacccagct
    37021 tatgcagatt aagactctaa gcgaactaga aaatgttata accagtgttt ccgcttaaaa
    37081 tagagaaaca ttatagccgt tggcagaagc ataataataa tgacacacca gaagccataa
    37141 cggttattaa taaatggcat ccctatgatt gtattcatgc cgaaaattgc agcaataagg
    37201 ttaaggggaa gaataattac cgaaaaaatg gttagggttt tcatgatctg attaatttgg
    37261 tcgttaagca atgattcgtt ggtgtcatgt aatacttcaa tcatttcttt ttgattatct
    37321 agattttccc agattctttt agaatgttct actagggtag aataatattt tttgagctca
    37381 gtggctggca ccaagctgct tttaatggta gttagggttt gtaagatatt tttatgattt
    37441 tgtagaatac ggcggatttt aatgatatta tttcttaagg ttaaaagttc ttgaacggtc
    37501 tcgcgttgat tactgtcaac tttaaagaca atgtcttgga cagtggcaat acgtttgctg
    37561 tgttcatcaa gaatttcata gcaataatgc ataagttgct ctaaaatttc atagagcaag
    37621 atagaagatg actctagctc ataggccaaa gtagaatttt cacttttacg acaaagattg
    37681 aaaaagttat tgagtggtgg cagatcacca ttgtgcactg ttactaaata tccatgaccc
    37741 ataaaaaaat caatttctga aggcataatg aggccatttt taataatagg aaaatggagg
    37801 acaataaaga tgtaatcttt ttccttttca atgatggccc tttgagcaat actacgagag
    37861 agagattgtt ttagatgatg caggttaaaa tgttggcgtt ttaataaata ttctatctca
    37921 gtcttaccag cagaattaat gttgatccaa ttcaccgcac cattagtttt agggttatcg
    37981 ataacaagct cttcaatctt tggtgaaatt tttaagtatt gtcccatata atttagttat
    38041 tgatttttcc ttaagtatag cataagataa taaaaagtga taattttatc ttttaaggag
    38101 aagttgtgtt ataatacctt tatataatac tatatttatg ccccaagaac gattacgatc
    38161 taataaggtg gaacaacccc taaccagtcc tgaagttgag caactagcta gtcaggagaa
    38221 aaaaaaagag ggagtgcccc tgcctagtcc agaaaagcca gaatccgcag cagtttcggc
    38281 ttctattaat tcgccatctg catatctaac tggttcgata gcgccactta ctcccagaca
    38341 agcagctatt gatgatattt tagaagatgg tttaaccgat atttatctat ctttacctcc
    38401 agacaagcag cgtgaactta aagaagtggg tgagcagaca gtgcgacaga ttgatagttt
    38461 attagaccac gttaaattgc agtggcggaa gattatgaat ttaattcgtc gctttttgtc
    38521 tattattcca ggaattaata aatttttttt agagcaagaa gttaagataa aaactgaccg
    38581 cattattgct ctaaaagata aagaataatt atggatccgc tcataatttt gggaggagtt
    38641 ttattaactg ctattattgt ttggctagtg gtttttattt ggcgaacaat agtgtggcga
    38701 tggtatcagg ctcatgctta ttttgatcaa gtagtctatc tagtgaaatt gcccaaagat
    38761 cggcccaaag accagaatca agactttact gcacaacaga tgcgggaaga gatttcttgt
    38821 gcggaaacta tttttgctag tattggcggc ctaagagctc agagagggtg ggcggtgtgg
    38881 ctttggggtc gggatgacca tttttctttt gaaattgttg ctaatagtag tattattgct
    38941 ttttacgtgg tagctccccg acgcatggct cgttatctag aacaacagat taatgcccat
    39001 tatcccgatg cagtattaga ggaagtaatt gattacaaca tgtttacagc tcaaggggct
    39061 attgctattg gttcccttaa aaccactaaa cattatgctt tgccgcttaa aacatataac
    39121 caagaggagg ttgatttaat gaattctcag cttaatgtga tgtctaaatt atccccggga
    39181 gaaagcttag ccattcagta tactgttcgt agtgcttatg ggagttggca tggggcggta
    39241 aaaaagattg tccaattggc ctacaaaaag aaatcggtaa gtgcggcact acagactagt
    39301 atttggtcat caatagctga tttttggtcg gatgctttta gcagtaataa ggctaaaaag
    39361 gaggaagcag ccgctaaaca agccgagaaa cgtttgacag ccatggaaga agaaatgtta
    39421 aaattaatgg agcaaaaaaa ttctaaggct ggacttgatg ttaatttacg tttggttgct
    39481 tgtggcaata atcaagggcg agctgatgct ctgctaggtg atttagctaa tgcaatgagt
    39541 cagtataatt attatgaata cggcaatagt tttcaggcta aaccacggcg taagccagtc
    39601 aaaaatttaa ttaaacagtt tatttatcgt cgttttgacg agaagtctaa atttttactt
    39661 aatactgaag aattggctag tttatatcat tttcctttac ggaatgctga aacacctaat
    39721 attttgtggc tgtcagctaa gcaggcatct gcaccatcgg acgtcccgac cgagggttta
    39781 cttttgggct ataatgtttt tcgagggatc agacgagaga ttcatattaa agaagatgac
    39841 cgtttgcgtc atgtttatat gattggtaag tcaggtgttg gtaagtcggt gctcttagct
    39901 aatatggcca ttcaggatgt gttgaatgga cagggtcttt gtgttattga tcctcatggt
    39961 gatttggtta atgatatttt agctagagtg ccagctgaac gggctgagga cgtagtgctg
    40021 ttttctcccg ccgatctaga gcggccaatg ggtcttaatc tcttggaatt tgatcctaag
    40081 tatccagagc agaagacttt tgtaattaat gagatgattg gtatttttga taagctttat
    40141 gaccttaggg cgactggtgg accaattttt gagcaatata tgcgtaatgc gatgctttta
    40201 gttatggctg accctgggtc aggttcaact ttaatggaaa tcccgcgggt tttagcggat
    40261 gctgattttc gccatatgaa attgggccgt tgtgctgatc ctacggtagt tgatttttgg
    40321 cgcaaagaag ctgaaaaagc tggcggtgat gctgctttgg ctaatgttgt tccctatatt
    40381 acctctaaac taacttcttt tatttctaat gacatgatgc ggccaattat tgctcaacag
    40441 aaaagtgcca ttaatttccg cgagattatg gatcagcaca aaattttatt agttagtttg
    40501 ccgaaaggac tagtgggcga aatgaatgct tatttactag ggatgatttt ggtaggtaaa
    40561 atgttagcgg cggccttgtc tcgcgctgat atgcccaaag aagaacggtg taattttttc
    40621 ctctatattg acgagtttca aaattttact actaattcta tttgtcagat tttatctgaa
    40681 gctagaaaat atggccttgg tttaatcatt gctcatcaat atattggaca attagttaat
    40741 cggggagata cagctattaa agatgcagtt tttggtaatg ttggcactct aattagtttc
    40801 aaggtcggtt cggaagatgc tgaagttctc cagaaagaat ttgcgccaac ttttgatgct
    40861 tatgacctta ttaatgtaga gaaatataca gcttatataa aacttttgat tgataatatg
    40921 tcctcacgac catttaccat gaagacccca tggccattac tgggtcatta tgatcaggag
    40981 atggctgaaa agattcggga gttaagccgt cttaaatatg gtcaggatcg gtctttagtt
    41041 gaggccgaaa ttattcaacg tagcagaatg aatcttaatt aatatggcac aagaaccaag
    41101 tattataaat aaagaggggg atgagcaaga atggcaacca gatcaaacaa ttagctttag
    41161 tgatcaagcc agtgaaatac catcagttct tccaccagca accggtagtc aaccccttaa
    41221 ggataatagg gacgaaggat atttgttatc agggaagcaa attaaaattt tacagtctat
    41281 agctaaaaag ttaacaacat tggcagctga tcttaatcat ctggttcaat ctttaatggc
    41341 tagtaatact tcagaggaag tgttagctgg tcagtctggt cttactattg aaggttattt
    41401 taatggcgag cagatggtgg ccagcaatgg acaggtttat gaggtgccaa gtaattatgc
    41461 gtctaagtct aagctggtag aaggagataa tttgcgatta actattgatt ccaatggtca
    41521 atttttatat aagcaaattg ggccagtggc acgccgtcgc ttagtcgcta ctttacagca
    41581 gggctctgat ggtaattatt atgccgttca tggtgagcaa cggtggcggt tacttaaggc
    41641 tagtgttagt tattttcgag ctgaagcggg cgaccgtgtt actatcctta tacccaaaga
    41701 taaagaatct caatatgcag ctttagaaaa tttagtactt gagtaatatg atggttgggc
    41761 gatggtcaag tagaaagatt ttacattatt ttcccagttt aacggctgaa caggagtcat
    41821 tattatcggg tcagccttat tttattcatc cttgtctttt attaaaaatt gctgaccggc
    41881 agcacaactt cagtaccatt agccacttgc ctagcaataa acaggtgcgt atggcttttg
    41941 agacccaggc ctccttatta ccgatgaaag aaattttaga ctatgactgt aaaggtttaa
    42001 ctattagtga agcagcagct aattggcaac aatttttaga taaaggtcat ttttctagtc
    42061 tatcagccct gaaaaaagaa ttatataaaa agcaatttgt cgatctggat tctacgatgt
    42121 ataatgacca tgccaatgcc atcattgggc aattaactga taaattcgtg aaggctatgt
    42181 gcatcatagt ttgcaaatga aactggggat ggctgcattt ctatataatt atatggcctc
    42241 tattaattct ttaatttctg acctagaatt agataaatca ttaattgatt gtctaaaaaa
    42301 tcgtgatttg gatcaaaaat ttttttatac cgaagaagga gctgatctat attatagttc
    42361 ttatgaacat tcagctaagt tagtgccggt tgatttttcc ttacctaagt attcggttgg
    42421 tgctattttg tttaatttct tttttctctt taataaaaaa gtggttatta aggcccgcaa
    42481 cgaagtaatt cattttttgt cagacaagag gttaaattta ctcaatatcc gggcctatta
    42541 tccagatact ttaaataatt ttttaaaaga gcatgggttt caattagttc aatctaaagt
    42601 tgatcaaaat ataaagcatg ggttttttat ttaccagagg gtttaatata tgtcaacatt
    42661 ataccgtcaa tatcggcccc agacttttgc ggaagtagtg ggtcagaatc atataaaaat
    42721 taccttagcc gaagagatca aacggcacca gattgcttca gcttatttat tttgtggccc
    42781 tcgggcggtg ggtaaaacta ctattgctag actattagct aaatccctta attgtcttaa
    42841 tcgtcccgat aactctgctg aaccttgtaa taattgtgct aattgtttag ctattacggc
    42901 tggacagtct ctagatattg tagagataga tgctgctagt cagactgggg tagacaatgt
    42961 acgggaaaat attattgcca gtagccgttt agccccaagt caaggaaaat ataaggtgtt
    43021 tattattgat gaagtccata tgctatctac ttcatcattt aatgccttac ttaagactct
    43081 agaagagcca ccggagcaag ttatttttat tttatgcact acagaagttc ataagatccc
    43141 agccaccgta atttcgcggt gtgaacgttt tgattttaaa aaaattagcc aagccgatat
    43201 tattaaaaaa ttgcattata ttttaaagca agaaggctgg tcagctgatg aagcagttct
    43261 aacggcgata gctaaaaatt cgcaaggtca tttgcgagac gcggaaagtt tgctgggtca
    43321 gttgatggcg gttagtcagc cgatgaaaaa cggcgagttg catttaacaa tggaggattt
    43381 ttatctttta gctccgagca gcgatttagt aattattagc cagtttatta ccgctttaag
    43441 tgagcaaaat tcagttgaag ctattaccat cattaatcaa ctggtggccg atggccgaga
    43501 tgttagagtt tttttacaag atttaattga aactttgcgt cagctattgc taagtaaaat
    43561 caatcctcaa ctactggtag aatatcaagg acaatggggc gaagatgata tgtttggatt
    43621 attaaagcta cggcaaaaat tgagcattaa ccaattagtc caatttttac aactttttat
    43681 tcaagctttt aatgagacca aagaggcttt tattatccag ctgccagtag aattagccat
    43741 tgtcaaggcc ctggggccca gtgaaataga gaaagtatct aaaatagaaa aacctactaa
    43801 tccccctgag tcaccaagta atgattcatc acccttaaag aagtcaggag aagaaaataa
    43861 gaaaaaaaat gaagtgatgg ctcccttgtt aagcgaggaa ttattaagtc gttggccggc
    43921 cgtagtaaat gaaataaaaa atcataatct tggtctgtcg gtagttctta aagattgtcg
    43981 gccccaaata gaaggggatc agatttgttt agtttttagt tataaactac atcaagacca
    44041 ggtaaaaccg ctacctatta agcatttaat tgaagaagtt atggctaaag tttatggtcg
    44101 gcagttgact attactacta agttattaag tgaatccaga ccggctgagt cggctgtgcc
    44161 ccaaaataag tctaacaaga cagaagaggg ggctagttta gatagtttat tagaaatttt
    44221 taatggtcaa attgtcagtt aactattgtc ataggaaatg acaaactaac tgaattggct
    44281 tatctcgggt agcggtggag tgtgggggtt acttatattg gtgttctgag gcttgtctta
    44341 agccagattg tctagtcggg actactagag taatgtttta taaacccctt gatgatcttg
    44401 gtaatccgcg ccagtctttt gactggcaag atgatgcaaa aatttgttca ggctctttgg
    44461 ctgccttagc taatagagag ggttattaaa gaagagggaa ttgctcaggc agtgttaacg
    44521 agcattgcta ttcttggttt taaatatggc cgtcgttatt tggtcgatgg cagttgagct
    44581 aatgtgactc tggcgagtcg ctattccttg ctggctataa ccgggtcctt gtcaaagcct
    44641 tttagatatg atttaataaa ataacttttc ggggatcgtc taatggtagg acagcaggtt
    44701 ctggccctgc taatcgaggt tcgaatcctt gtccccgagc attaaggaat aaagattcta
    44761 gagggtttgt ttttttgtta tctgagccaa aa
//
LOCUS       DAOOIS010000004        38983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_1142_length_38983_cov_47.113517, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000004 DAOOIS010000000
VERSION     DAOOIS010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 38983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38983
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1142_length_38983_cov_47.113517"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..335
                     /locus_tag="PK960_01030"
     CDS             159..335
                     /locus_tag="PK960_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07793.1"
                     /translation="MSIETLFKKSMSEKFLTPEKQIQPENIDLDKTLAELSTHTAYQK
                     KIKKLLITLFFINN"
     gene            complement(461..1747)
                     /locus_tag="PK960_01035"
     CDS             complement(461..1747)
                     /locus_tag="PK960_01035"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPD07794.1"
                     /translation="MKKVALIYLVVFIITLGGQAAAQNITWHNSNLEACQILDQNNYF
                     VAGWTNTMVFRFMKSTDAGQNWQSVEVSHPDQPFCVNGLWMIDDLVGWICGDSFGLYK
                     TIDGGNTWTDMSNCPGIRNNSSSLLRIKFTSPEIGYILSSGDLKKTINGGNTWTTIKA
                     NQVEAGEGGFRGFDVAGETIILSAIRYNVNDLSNCYVSQDAGQTWQLSFVDRSDFSFF
                     MKIASPSTFYASITNRRELYRSIDGGVTWSLFLNPPNGGTLTILSWTNNTLYVVSGYN
                     GIIHILKINNGWQEVMSLGNIIVHGFDFKGNRGITCGDDGSIATFTDTTVVNSDPVTP
                     VTPSPEVACYPNPFQGSTNVKFNQIDNSPTTVAIYNTRGQLVRALVNEQKLSPGEHII
                     VWDGKNNSGQPTAAGIYLYKITSGRFSSTKKMIMMK"
     gene            1907..2128
                     /locus_tag="PK960_01040"
     CDS             1907..2128
                     /locus_tag="PK960_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07795.1"
                     /translation="MKKILIIFVYAGLGLVSGILASALWAQAAPVIFYESKLRIPNVT
                     NMTATTSIVTVQYLENLINVMLRPTYLNP"
     gene            2141..2827
                     /locus_tag="PK960_01045"
     CDS             2141..2827
                     /locus_tag="PK960_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07796.1"
                     /translation="MSKKTGLLICLVIFLSVIAYGLVQAGVVNFVDGINIQYETIYKD
                     QVPSKAEAENLLMPLSSSFFPSAHLEKNAGGCRIKSGTFYIDYTQVHFWQGYYWGSAT
                     AIATSTTDNSCICPTGTTRILRRSQANNQISELDTTWWKDFRWVNRGLGFGIQGELVW
                     TTQCTKFYNTCCPGGCMTGDPGCSCAYNLNNCKSLFSNGQGTGCGGSQPIYYTEYLYN
                     CVATSETLKQ"
     gene            2829..3491
                     /locus_tag="PK960_01050"
     CDS             2829..3491
                     /locus_tag="PK960_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07797.1"
                     /translation="MKKRLILVFATFFIGAFFYVTTHGATNVVSFPDGAKNLQTITAN
                     TDAIIKNWADTLIIGSLEKNAGGCRIKSGTFYIDYTQVHFWQGYYWGSATAIATSTTD
                     NSCICPTGTTRILRRSQANNQISELDTTWWKDFRWVNRGLGFGIQGELVWTTQCTKFY
                     NTCCPGGCMTGDPGCSCAYNLNNCKSLFSNGQGTGCGGSQPIYYTEYLYNCVATSETL
                     KQ"
     gene            3493..4095
                     /locus_tag="PK960_01055"
     CDS             3493..4095
                     /locus_tag="PK960_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07798.1"
                     /translation="MKKRLILSGATVLLLIILASIFFILAKKYSPLISHQPLNNNQNN
                     QLETPNIESIGSINVDNQNFDIKLNAIKDGIVVAENGQPTRNDSEPKSPGTPMVSLDI
                     NGAEGINIPNDVIKISITKDSISPKPIIISAGKPTTFMVESADEWYHVLRFADNSLKA
                     LAVGVAGHETRLIRFNAPTTTGDYIIYDEIFGVDGLVTVK"
     gene            5032..5733
                     /gene="truB"
                     /locus_tag="PK960_01060"
     CDS             5032..5733
                     /gene="truB"
                     /locus_tag="PK960_01060"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPD07799.1"
                     /translation="MIPNNQALPYPSGFLLIDKPSGPTSHDIINNLRHLTGIQTIGHA
                     GTLDPLASGLLIVAIGRTATKQIPNLVKQDKVYKAIICLGGLSDTDDSQGKIQPFTNC
                     HQPTKQEIKIILNSFRGEQNQIPPMYSAKKIHGERLYKLARKGQVVQRQPNLITIYNI
                     NLLHYEWPTLSLEIHCSAGTYIRAIARDLGKMLGCGGYLCQLRRTKIGNYSIQQAVTL
                     EKLTSHNWTKFLFNL"
     gene            complement(5737..7536)
                     /gene="glmS"
                     /locus_tag="PK960_01065"
     CDS             complement(5737..7536)
                     /gene="glmS"
                     /locus_tag="PK960_01065"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPD07800.1"
                     /translation="MCGIIGYCGQHRASEILIEGLKRLEYRGYDSAGLCLVENNNLVC
                     YKRVGPVAHLEEAVARQDLGGHVGIAHTRWATHGIPSEVNAHPHFDCHHQIAIVHNGI
                     IENYQALKELLISEGHIFSSETDSEVLAHLIEKFYKGSLEEAVTKALALVEGSYGLVA
                     LHASENKLVAARRSSPLIIGVGQDEIFIASDVPAILNHTKDVVYLQDGDLAIIDSKGY
                     QIKNLAGKLLDRQVEEVKLSMEQIEKKGYKHFMLKEIFEQPQSLANVLAGRITDNQIK
                     LSLNWHREVIQRIIIAACGTSWHSALIGKYLLEEIVGLPVEVDYASEFRYRHPLVSSH
                     DLFIAISQSGETADTLAALREARSRGAQSLGIVNVVGSTVAREVDSGIYLHAGPEIGV
                     ASTKAFTAQITALLLLALYWRHEAGYEIDKKILNELRHLPTVAEKTLEANSQVIELAQ
                     RFMNFENFLYLGRGLNFPVALEGALKLKEISYIHAEGYPAAEIKHGPIALVDENMPVV
                     VLATKNDLSDKTLNNIQEIKARGGQVIAIVDQDYEPVKKLADYLIKVPVVHVWLSPII
                     NVIPLQLLAYYIADAKGLDVDRPRNLAKSVTVE"
     gene            complement(7540..9531)
                     /locus_tag="PK960_01070"
     CDS             complement(7540..9531)
                     /locus_tag="PK960_01070"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPD07801.1"
                     /translation="MSIAIIVIIASALLAIIYGIIAIYSILKLPRGDEKMQSIASAIQ
                     AGARAFLNRQYRTVGLVAILLFFIIGLLPGLGWLTALAFIIGAILSALAGYIGMNISV
                     RANITTTEAAKSGEKTALAVAVRGGSITGMLVVGLALLGLASFYFITHDIHALVGFAF
                     GGSLISIFARLGGGIFTKAADVGADLVGKIEAGIPEDDPRNPAVIADNVGDNVGDCAG
                     MAADLFETYAVTSIAAMILGGLIFGTDNQLLIILPLLLGAVSILASIIGVYFIRLGKS
                     LNIMAALYRGLVITAVIAAIIFYPIIVWAAKDNGLFTINNLYLAAVIGLVTTGFFVVV
                     TDYFTSTKHGPVKQIAEASKAGHGTNIIVGLAVSMKSTAGPIIIVVLSILGAYCLAGL
                     YGIAIAAVSMLSLAGIIITIDAYGPITDNAGGIAEMAKLGEEIRNVTDSLDAVGNTTK
                     AVTKGYAIGSAALASLVLFADFSQKTQGSQFLIDNPYVLIGLFIGGLLPYYFGALAME
                     AVGKTAGSVVDDVRMQYKEKPGIITNTEKPDYGRTVDIVTKAAIKAMIIPSLIPILAP
                     IVVGFIFGVPALGGLLIGSIVTGIFVAISMTSGGGAWDNAKKYIESGNCGSKGSEAHK
                     AAVTGDTVGDPYKDTAGPAINPMIKIVNIVALLIVDFLI"
     gene            complement(9591..9661)
                     /locus_tag="PK960_01075"
     tRNA            complement(9591..9661)
                     /locus_tag="PK960_01075"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9627..9629),aa:His,seq:gtg)
     gene            complement(9670..10221)
                     /locus_tag="PK960_01080"
     CDS             complement(9670..10221)
                     /locus_tag="PK960_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07802.1"
                     /translation="MIWLQVLGLLLILVLLTFLVEEFFNLIFKGYAPLVSSRSKVLDE
                     AVQQFISLDLPAGAKVYELGCGRAGFLRHLEKTIEDKEKYKLVGIELLFSVYLLAKLQ
                     LFLVGSKIKIIRKNFLKMSLKDVDCFYCYLNGPMMARLSLKLKKEAKPGAILISNQFE
                     LPGYEPIEEIRLGLTNCLRIYKL"
     gene            complement(10247..10318)
                     /locus_tag="PK960_01085"
     tRNA            complement(10247..10318)
                     /locus_tag="PK960_01085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10283..10285),aa:Arg,seq:tct)
     gene            complement(10360..10749)
                     /locus_tag="PK960_01090"
     CDS             complement(10360..10749)
                     /locus_tag="PK960_01090"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07803.1"
                     /translation="MNNFFGQQLVRKKDLWSFPRIIRNILVALLIITIVIYIIFSLKK
                     IFSPPRLKIISPQDNIATTERQILLSGQTDPEVQIDVNGEIIISDESGYFNRPINLHL
                     GTNYLNIKAIKQYGGQTEITRQIILLH"
     gene            complement(10772..11182)
                     /locus_tag="PK960_01095"
     CDS             complement(10772..11182)
                     /locus_tag="PK960_01095"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07804.1"
                     /translation="MKFTKFIFLVLSIMMLAWPTISFAAAENTKQLVPTEHYEEGDYT
                     IQDFTKMAIFVGKVILGLAGSAALVMFIVGGIQMIIAAGNSENFKKGQQTLINALIGL
                     VVIFCSYIIINFVLNVVNVAPWARNTLDQTIKKE"
     gene            complement(11190..12593)
                     /locus_tag="PK960_01100"
     CDS             complement(11190..12593)
                     /locus_tag="PK960_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPD07805.1"
                     /translation="MWKKISLLSLLAIFFVTSGFGCRLQSHEVKEAMQPITLNYWRAW
                     DEPDDFQEILAKYQALHPNITINYRKLRYEEYEKELINALAEDRGPDIFSIESTWLPE
                     YKNKIAPMPPSISMVYPIQKGTIKKEVIYELKTTPSLSLKDLRARFVDVVASDVVMDN
                     KIYGLPLSVDTLAMFYRRDLFNNKGIINPPAYWDKNFQEVVKKLTEKDIEGNIVQAGV
                     AIGSGANVKRSADILAALMLQSQVPVIQNGQVVFNKKFGDRYPGLEALRFYSDFANIN
                     KEVYCWDKAFDNSLDMFIRGQAAIMFGYSYDLPLIVAGRRSDFNGSNATDYLQDLNIG
                     VSKLPQLASTTQPVNIANYWVETVSKKSQHINEAWDFIQFITRAENVTSYLDKTKRPT
                     ALRSLIEQQLSEEYLDVFASQLLTARSWYKGYDAKSAQKIMAEMIDNVVDNPDQLQAI
                     ANLGASKLSQTLLPPPE"
     gene            complement(12691..13653)
                     /locus_tag="PK960_01105"
     CDS             complement(12691..13653)
                     /locus_tag="PK960_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPD07806.1"
                     /translation="MKKLLLISLAMVLVLTGCSARPKTLKPDEAKEKAETFINAVLMA
                     PGSQAIVKEIEKMDGFYKMQVDVGSGEPIESYLSLDGKLFFPQALDIDAINEQLAAAT
                     SSNENGGIATVEQKSDKPTVDLFVMSYCPYGLQMERGILPVVEKLGDKIDFKVRFCDY
                     AMHDKPEIEENLTQYCIQKEQPDKFLSYLNCFIKSGDAASCYKEAKIDEQALGTCTSS
                     VDKQYNIMSSFNDKNSWNSQFPPFNVDKADNDKYNIQGSPTLIINGQEVSSNRDSASL
                     LKTICSAFNNAPEECSAQLSSSAPAAGFTADNSSGSGSSGGNCQ"
     gene            complement(13711..16251)
                     /gene="gyrA"
                     /locus_tag="PK960_01110"
     CDS             complement(13711..16251)
                     /gene="gyrA"
                     /locus_tag="PK960_01110"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPD07807.1"
                     /translation="MTVKKSSVVVDNSADNKLNEVKKLKEETGSAVPSGSILVQPIVD
                     EMRQSYLDYAMSVIVSRALPDVRDGLKPVHRRILYAMWLLGLHSGGKFRKSATVVGEV
                     LGKYHPHGDSAVYDAMARLAQDFAMRYPLVKGQGNFGSVDGDRPAAMRYTEAKLAAIA
                     EEMLIDLDKDTVEMIPNFDGSLTEPQVLPAKIPNLLLNGCMGIAVGMATNIPPHNLRE
                     VAAAIEKLIDHPEATIEELMECIKGPDFPTGGFIYNHEDILRAYTTGKGGIVMRGRAE
                     IVENSAGNFQIVISEIPYQVNKAVLVERIADLIKDKKIEGVKDLRDESDKEGIRIVLD
                     LKRDSYPKKILNALYKQTPLQETFHVNMLALVNGLQPRILNLKTILEEFVKHRQEIIR
                     RRTTYDLIKAKERAHILEGLMMALARIDEVIAMIKASKDKDRARVNLMKHFKLSEKQA
                     VAILEMKLQTLANLEQLKIETELKEKHKAIADLEQILSSESLVSNIIKQECRDMAEKY
                     GDNRRTTIMNQDIKDFRAEDLIPNEEVVVIMTRDGYIKRLPGNTFKVQGRGGKGVLGL
                     TTKEADAVQFLFTTMAHGDLLFFTSRGRMFELKAYELPLASRTAKGQAIVNFLQLASD
                     ERITAILPLDKMGDKEYLFFATRQGLVKRVSVDAFANIRRTGLLAIKLKPDDQLIWVK
                     STSGSDQIQMITALGQAIRFKETDVRDMGRGASGVIGMRLRKGDHIIGMGVINTDPEK
                     IKDYQILSVTAHGYGKRTNLSQYKIQNRGGLGIKTAKITDETGEIVNAFVVDKKVMAN
                     KDLIVMSMNGQVIRIPFASVKESGRDTRGVHLMRFKEGGDQVVGVTWL"
     gene            complement(16261..16632)
                     /locus_tag="PK960_01115"
     CDS             complement(16261..16632)
                     /locus_tag="PK960_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07808.1"
                     /translation="MKKINSSKNARFNPRDWKPETRYLILWLGVTIIMLILLIFWFYG
                     LRSSLIMSNNESNARPLLNISQIQSDLSSGIEEVKSSTQALKKINTSTQDLTPEEKLL
                     EEQNKIKLNELRQKLEQLPDK"
     gene            complement(16641..17297)
                     /locus_tag="PK960_01120"
     CDS             complement(16641..17297)
                     /locus_tag="PK960_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD07809.1"
                     /translation="MIINWLGQSCFKFQDKINGQEIALVIDPFDPNYTGLKLPNNLSA
                     NIVAITHGHHDHNFIQGVQGWSYIIDTAGEYEIGGVCIDAIDSYHDAQKGKERGHNLI
                     FRLEFSDLTITHLGDLGHLLGPDQLERISGTDILIVPVGGHYTIDGKQAVEVINQVDP
                     KMVIPMHYQLPGLKFELDSLENFIKTIGLSPTNESRLKISRRDLDNENLQLVVLSPSV
                     "
     gene            complement(17404..20325)
                     /locus_tag="PK960_01125"
     CDS             complement(17404..20325)
                     /locus_tag="PK960_01125"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPD07810.1"
                     /translation="MTQLDDYLANLNQEQRRAVEHGTGPLLIVAGAGTGKTTVLVNRL
                     LYLIMSGQAKPEEILLTTFTDKAAGEMEERADRLLPYGYTDLWIQTFHSFGERLLRQH
                     GLDIGLPINFKLLNETSQWVLLRQNLDRLMLNYYRPLGNETKFLTDLVKHFSHLKDED
                     ISSQQYLDYVESLDLSDMNEPAKLEVTRLRELAEAYATYNQLLLDNSCLDFGDLIVYT
                     LKLFRERPNILEQYRQQFKFILVDEFQDTNWAQYELLKLLAAPANNLTVVGDDDQSIY
                     KFRGASLANILKFKDDFPEAREIVLTANYRSGQAILDQAYRSIQYNNPDRLEVKLSLN
                     KKLVAVGIPKSSVDVWPFHTESEEVVAIADYIKEEHQTSDEVQWSDFAILIRSNRDAD
                     KFINELMRRQIPCQFMSMRGLYYKPIILDCLAYFKLLDNYHESTALYRVLNSPPFALP
                     AVDLIELTALAHRKSWSLFEAMKHNDLAFKISPAGREIMAKLLRLIDEQSLMVAKEAP
                     SRLFVDFVYKSGLLSGLDPDKDWQIFHYLNQFYRKIKDFENGGQDLRLKDFITLINLE
                     LEAGETGSLNLPLEDEDVVKIMTIHSAKGLEFRYVILPDLVDKKFPTIKQGDSLPIPA
                     ALIAEPVGTSDMHLAEERRLFYVAMTRAKEKLILAYARDYGGARDRQPSIFLRELDLT
                     NNITERQLDSTQVFELVREVESLSKPTPELVKTEQPLPGNKFSFSQFEGYSNCPLQYK
                     FNFLLKVPVPEKANTIYGRLMHRSLYEIFLPCLKSSGGVDLFGQRATPEYLDWPDWLD
                     IYKRNWVDEGYNSAEERDQYRKLGEQSLRLIWQKVQDEGWPKVLGLEKQFTWKGEGFI
                     LKGTIDRIDQLPDGTVEIIDYKTGQPKESLDYKTKRQLILYKVAVEEVFNWPVGKLTF
                     CYLNNGSFVSFEAKAKDVDKVQHEVAETIKAIKQGLFPARPSQLCSYCDFRSICQFRQ
                     V"
     gene            complement(20327..21616)
                     /locus_tag="PK960_01130"
     CDS             complement(20327..21616)
                     /locus_tag="PK960_01130"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPD07811.1"
                     /translation="MWQRQFNDNFPLVLKVPLDNCQTFTILVIVATGSKFETAQEKGV
                     SHFLEHMFFKGTTKRPTTLAISSELDAIGGEYNAFTSKESTAFYVKCVANQAEVALDV
                     LSDMLLNSLFASEEIDKERGVIIEEINMYEDNPLMHLSDVFERVLYGDTPAGWDTAGS
                     KETVNSLKRENLIDYWQTHYGHSRAIICLAGNLSPDVDTLVNKYFSQWPTTFNFRDKL
                     PVINQQTEPQLLVENKTTDQVHLALGVPAFPYGHPAELPTKLLAAILGGTMSSRLFLE
                     IRERRGLAYYVRTEAETYTDSGYLVTEAGVKKESLPLAISMILQEYKKLTTELVEEGE
                     LNRVKKFLSGRLILGLEGSDDLARWYGYQQALRLGQGDNQAILDTPQDYLTKLNSVSA
                     TDIREVARQIFVDKGLNLAIIGPVANQNKLVSLLTLD"
     gene            complement(21621..23003)
                     /locus_tag="PK960_01135"
     CDS             complement(21621..23003)
                     /locus_tag="PK960_01135"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPD07812.1"
                     /translation="MSDIDLDSLVSLCKRRGFIYQSSEIYGGLAAVYDYGPYGVELAN
                     NIKQLWWKDLVYGHDNIVGLDSAIFMHPKVWRASGHIANFSDPLVECRHCHSRFRADE
                     LLAQAGVKANETMSENEFNQLFDDYRHLVVCPDCGQQNFTAVRRFNLLVESNLGDFTG
                     SGAEPVYLRGETCQGIYVNYKNIMDSTRLQLPFGIAQIGKAFRNEITARQFIFRTREF
                     EQMELQYFVKPTEDLEEFNQWKEQRWNYYINNLGISTNNLQWHKHEKLAFYAKAAFDI
                     EYKFPFGFQEIEGIHARGDYDLSQHQKYSGVKLDYYDPISHETFIPHIVEASSGVGRV
                     FLMLLAEAYGEENLPDGSKRIVLHLKPALAPIKVAVFPLLRNRPELVAKAREIFDQLK
                     VHWRCEFDDNGNIGKRYRRQDEIGTPYSITIDFDTLANNVVTVRDRDSMKQESINVNE
                     LIAYLSSKLN"
     gene            complement(23107..23781)
                     /locus_tag="PK960_01140"
     CDS             complement(23107..23781)
                     /locus_tag="PK960_01140"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HPD07813.1"
                     /translation="MSIYPIPAKLPDAANWLKFDLAKFSNSELLAFIISQNQEIKSSI
                     ALAQRVLKKIPAPCLVRASFEELVKAGLNSEQANNFLAAVEMTRRFCRQRLKNIYYSD
                     EVIYQEFKYLRCCTQERLVVGYLDHRCHELRRKSFLFSQMRQPDFSPCEIFNPILKLP
                     AQQLITASNHLSGGAEPNEVDLILFNKLLKICEAFGLEMLDNVIFSRCSFYSFRKAGR
                     LKTSTG"
     gene            complement(23931..26201)
                     /locus_tag="PK960_01145"
     CDS             complement(23931..26201)
                     /locus_tag="PK960_01145"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPD07814.1"
                     /translation="MESTEIVVSRDWHGRQLTIKTGRLARQADAAVTVQYGDTVVLAT
                     VVEAKEAREDGDFFPLMVDFEERLYAAGIIKGSRWVKREGRPTDEAILNGRMVDRAIR
                     PLFDESSRRDVQVILTVLSVDQTNDYDIVALIAASAALRISGINWQGPIGGVRVGRIN
                     GELVYNPTYKEREQSDLDLIVAGTRERVIMIEAGAQEVPEEEMIKAILAGEQALQGSI
                     DLIEELYHRVGPRGRKQEKPIRLSAEELQAQELREKAQKMAEDWLAHNAEPILFDKIY
                     YTKGQRKLAVDTIKDKLDEYLFSQNIEKNLRQALISQIVDPYIEVIVSKNILEADRRV
                     DGRALDEIRPMLADVSILPRNHGVGLFSRGETQVMSIVTLGSPSDEQSLEGLEGVSTK
                     RYIHHYNFAPYCTGEAKPIKVAGRREIGHGALAEKALVPVLPSKEEFPYTIRVVSETL
                     SSNGSSSMGATCASSLSLMDAGVPIKRPVAGIAMGLASNQDMSQWKVITDLQDLEDGV
                     GGMDFKITGTSEGITAIQLDTKTCGLNEEIIRTAINQGCKARLQVLEVMKQAIAEPRA
                     ELSPYAPKIVTFFIDPDKIRDVIGSGGKIINEIIAACDVTIDIEQTGQVTVCGNNKDK
                     LDLAVQWIKNIAHKFAVGEEFVGEVVRLLDFGAFIELLPNHDGMVHASELAPFYVSKP
                     SDMLNIGDRVSVRIKDIDDQGRINLTMKDLPANEAIWERYKAKGLPADRSLGSSRRRP
                     TSPRHGDNFRRRSYRQ"
     gene            complement(26282..26779)
                     /locus_tag="PK960_01150"
     CDS             complement(26282..26779)
                     /locus_tag="PK960_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPD07815.1"
                     /translation="MVKHPEQRVGVLVDVANLYHSARNLYGKKVNFHKVLTEVVGERK
                     LIRAVAYVIRSDNNDEMAFFEALSQQGFEVVMKDLQVFPGGVKKGDWDVGISIDAIKL
                     SHKLDVIVLVSGDGDYAPLVNYIQSTTGCLVEVAGFRQTTSAKLLEIADDFINLSSHQ
                     FLINH"
     gene            complement(26913..27176)
                     /gene="rpsO"
                     /locus_tag="PK960_01155"
                     /pseudo
     CDS             complement(26913..27176)
                     /gene="rpsO"
                     /locus_tag="PK960_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888875.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
     gene            27304..28260
                     /locus_tag="PK960_01160"
     CDS             27304..28260
                     /locus_tag="PK960_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPD07816.1"
                     /translation="MIQDDINKFLEYLEVDRGLSNNTLISYRFYLARFANFAKNQGIH
                     NVSQINQELIHRYRLYLNRLNNRQDENLKKNTQNYHLIALRQFLKYLSKNNIESLDSN
                     KIELAKQEPRQVSFLEGYDLAALLQAPMTAKQPNTLIKLRDRAILELLFSTGLRVSEL
                     ARLKINDINLKKSEFTVRGKGNKLRLVFLSEEAKSALKKYLEARQDTEPFLFISHGKI
                     NQASSSQYGLTPRSIERLVEKYALAAGITKQITPHTLRHSFATDLLINGADIRSVQQL
                     LGHASITTTQVYTHVTDQQLRQVYQNFHGKNRKNIANDKSQK"
     gene            28324..28902
                     /gene="clpP"
                     /locus_tag="PK960_01165"
     CDS             28324..28902
                     /gene="clpP"
                     /locus_tag="PK960_01165"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPD07817.1"
                     /translation="MTLIPTVIEKDGQIERAYDIYSRLLKDRIVFLGEPIDDHIANLI
                     IAQLLFLDAENSNKDIKFYINTPGGSVTAGLAIYDTMQFIKADVSTICVGMAASMGAV
                     LLAAGTPGKRLALPNSEIMIHQVMGGFEGQATDIKIRSEHILKIKEKLNKLLAQHTGQ
                     EIKTIEKDTERDHYLTPLEAKNYGLIDKIITR"
     gene            complement(28918..31038)
                     /locus_tag="PK960_01170"
     CDS             complement(28918..31038)
                     /locus_tag="PK960_01170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD07818.1"
                     /translation="MPCYNEADSIGNVLKRLPANVEAIVIDNNSTDGTAKIARQLGAK
                     VYRETTQGYGAAIRRGFKEAHGDILVVLDGDGQYPAEAIPDLVNALEQEKLDFISAAR
                     FPLQDKTVMPFIRRFGNWCFNRAVRVICGLKLKDSQSGMWVFRRSVLEKINPQHNNMP
                     FSEEIKILAAKNKDIAFAEKPIPYAARTGNSKLVPWKHGWMNLKFLFQLRGRLEDKGP
                     DWLTIGGLAIVLLIYILLSLYKINTPFIHVTDDVAGANGVAAINLNHYGWFNLKFGFF
                     DRWGAVGLPYTHHPQFFLLPTTVFYAIFGVSEATTRLGLLFCMIVGLTLFYFALFKIS
                     HRRSFTFLTTLIFALLPGMIYYGRSFELTVFTIPTALISFSLLVFYARGNPKRRSLYF
                     ILFWLSVVVGGLFAWFYYLFAAALWVFILLFRAGRKIPARKYLLIFIPITLVGLFAFN
                     LLHFYILKGPSFLTDLRGAYGIRTDSSIPFSNWLKTIVVDRGELNYTWLFLITAGLGL
                     IWFLVDYFSRHIPEDFGWLFVWLIAPLAVAWRFYQWITHPFGVIVFAPAVAIFSAYLL
                     YRLYQLIRPLGLVVAGVVIIVGAYLSWQNLNFFYNQFIILGDRDIKLLKELAPTIDDG
                     QLCLGDNQFGLGYNGIVEWYLRKRLGDDPQACSLALLFRVGVTQDFVDQQTTYYSAAG
                     FNAVRTCGDFWCLLEKTPHSKIIQ"
     gene            31329..32843
                     /gene="rny"
                     /locus_tag="PK960_01175"
     CDS             31329..32843
                     /gene="rny"
                     /locus_tag="PK960_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPD07819.1"
                     /translation="MNYIIGLVAIIVGLILGYWLQHQLTIKRAANAEHKAENIINEAR
                     AKEKDILLKAQDKALKLIEDAKQENEARRKEITAWQNRLEKRETAFSQKILELQDKQQ
                     ELYDKIAEVETTKEKINQLKEEELVKLEEIAKLNTEEAKKLLLERVEKEASDSLMSRL
                     NKLNEEASEQIEEKSKNLMATAMERLASTFTAEMTTTVVDLPNDEMKGRIIGREGRNI
                     KTIEQLTGVEIIVDDTPNAITISGFSGIRRQIAKRALDYLIKDGRIHPTKIEDAIERA
                     KEELALDIKKAGEDALYELGITGFDPKLVQIIGRLKYRTSYGQNALKHSVEVAHLAGL
                     LAEELGADVTIAKKAGLLHDIGKAVDHEVKGTHPQIGGDIARKFNLPQEIIDPIETHH
                     DDHPQGLISVIVQVADAISSSRPGARNDSLDQYIQRLEELEKIASNFNGVDRAYAIQA
                     GREIRVFVKPEEISDLSAHELAVQIAQQIEQELKYPGEIKINVIRELRVIEYAR"
     gene            32848..34740
                     /locus_tag="PK960_01180"
     CDS             32848..34740
                     /locus_tag="PK960_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPD07820.1"
                     /translation="MSIKKIFDKTKNHKTSDQSLSQHVLDQEIIFRLSKGRLPGRRQW
                     HYLPKFLSKKEKIIIIICLIAILSSLSFFLYNYYQKHCEKIPQNGGYYVEGLIGTPRY
                     INPLYLTFNQTDRDLARLIYSSLFTRDAQGHLINDLAESYEVSPDHLTYIIKIKDNVK
                     WSNGQNLTAEDVLFTYTAIMDQAYGSPLRNSFSGLEINKVDDLTIKFTLSQPYNAFLD
                     LLTIGIMPRELWGQIPASSASLADLNLKPIGSGPYQFKSLTKDKAGNIKSYELTVNPY
                     YFRQLPYIPKLTFKFYNNYEELAAALGDGSIDGAAYLPQKTAEDLITTRRYDLKYLGT
                     SQLVAIFINSEDKVLKDKNIRRALALSIDKNKILLDNNNLIGHLATGPILNHNPYYDK
                     TIAEVSYNPTEAQKILTDSGWSLKTITTDDVSAAQQKLQNNQGDQNEAKTIIDLGPGQ
                     WLQKNNQYLTFNLTVVNEPDYLAIAQQIGLAWQKIGIKTYLDVVDPENINNQIIRRKN
                     YSLLLYGQNLGSENDLAAFWHSTQSGPNGLNLSNYRNEQVDKSLETLRTSIDEEAKKK
                     AAQTLQQQIATDLPAIFLYNPTYLYIQNKKIKGFAMEVIIEPADRFNQINEWYIKTKI
                     KWQKTH"
     gene            34741..34824
                     /locus_tag="PK960_01185"
     tRNA            34741..34824
                     /locus_tag="PK960_01185"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34775..34777,aa:Leu,seq:cag)
     gene            34877..36694
                     /locus_tag="PK960_01190"
     CDS             34877..36694
                     /locus_tag="PK960_01190"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPD07821.1"
                     /translation="MMTKYQTRNYASRQTGKSYSPSRKPRRTNYYQQTLNSTKSERGK
                     LKVIVLGGLEEVGRNMTLLEYDQEIIIIDMGLQFPEEDMLGIDYIIPNTDYLVGKEDW
                     IKGIIITHGHYDHIGAIPHLLGKLKNPPMFMGRLTAGLVEKKIKEQPQCPKAAITIIE
                     ENKAIQLGKNFNIEFLRMNHSIPDCFAPIIHTPVGTIMHTGDFKIDYSPVNDKPADLQ
                     RIAQFGSQGILLLMSDSTDSTHPGYQISESSIGDEMSRLFEKIEGRIIICTFASQISR
                     IQEIFTLAEKFGRRIYIQGRSMNDNIEIAKQIGYMKFNSQTIIHENEIHHLPDNKIIV
                     LGTGAQGENNAFLMRVVNNEHRTVRLKPGDTIIFSSSVIPGNERSIQNLKDQIVRQGG
                     KVIHYEMMDVHAGGHAKQEDLKLMMRLLKPKYFMPIEGSHFMLQAHAELAQQVGIPTN
                     NIFVADNGQVVTFEVRGHETIGQLTKEKVMTDYVMVDGLGVGDVSDIVLRDRKTMAED
                     GMIVVIATIDSKTGDPIGNPDIISRGFIYMKDNKDLIQDTRMRVKKIIKESDPLLLTG
                     TRGLGEDDQLKNKIRADVSQFLFNKTKRRPMVLPVVIKV"
     gene            complement(36808..37137)
                     /locus_tag="PK960_01195"
     CDS             complement(36808..37137)
                     /locus_tag="PK960_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HPD07822.1"
                     /translation="MEFKEYQTKSRRTAVYPVVEKGFVYPTLGLVGESGEVAEKIKKV
                     FRDGDGVVGEEQREAIAKELGDVLWYLAQLATELGLDLDAIAQNNLDKLLSRQERGQL
                     HGSGDNR"
     gene            complement(37142..37627)
                     /locus_tag="PK960_01200"
     CDS             complement(37142..37627)
                     /locus_tag="PK960_01200"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HPD07823.1"
                     /translation="MEFLQELIGSKELLTLIYSAIPITELRATIPLAITVWHVPPLTA
                     LILAIIGNSIPIVFIILGLNKLVELVEKHSSFFKKILESIFKRTRRKTWQKLEKYGLI
                     ALFMFVAIPLPGTGAWTGSIAAWLFGFPLKKAFWPIFSGVIAAGIIVTILTVGATAII
                     T"
     gene            complement(37635..38132)
                     /locus_tag="PK960_01205"
     CDS             complement(37635..38132)
                     /locus_tag="PK960_01205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPD07824.1"
                     /translation="MTNHSQKENNSSCLFCDIATGRLTTPGIFWEDSDFMAFLAIDPN
                     TEGFSCVIPKKHYDSDVLAMPDEVLCRFMVATKQVAQILKNYYADVGRVGVIMEGMGI
                     NHAHLKLIPMHGTGHLKEGKWRSYSSGPAQWFSTYEGWLSSASGPLADPKHLQELASK
                     LRASY"
     gene            complement(38263..38805)
                     /locus_tag="PK960_01210"
     CDS             complement(38263..38805)
                     /locus_tag="PK960_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07825.1"
                     /translation="MRSRTLKKQIKLFCQEVVINTNLVERLKRVEAEAYSIEAIVEFF
                     QIISPIQDSGRVSVLAETIEKNQKKVPQSVADAIRRLEKSVERCGRDPYGMNRTEKGV
                     EVNYDNVWLGDVYGLWTFPASYWLAHEKELKASVRPDLSYDDQEVTDWHCIMAQCFYF
                     VTGNVPLILEDIKVIMDYVS"
BASE COUNT        12884 a         8224 c         6520 g        11355 t
ORIGIN      
        1 agagcagttg cctcttaagc aaacggtcgc aggttcgaac cctgctgggc gtacattatt
       61 tccgctcccg atgcagttgc atataacgat tgtaaaaaag agtcgggaga aatccgtacc
      121 caaccatagc gctagagaga atttccagag tcctcaatat gtctattgag acattattta
      181 agaaatctat gagtgaaaaa tttttaacgc cagaaaaaca aatacaacca gagaatatag
      241 atcttgataa aactttagct gaattatcca cacatacagc ttatcaaaag aaaataaaga
      301 aattgttaat aactttattt tttattaaca attgattcca attctcttga tatttttgtc
      361 aaatgaacat ccaagattaa ctcaagaatt aataaagctg aaataaaaaa accgtcagac
      421 tagcaaaccg aggcttgcgt cgtctgacgg gattagtaat ttatttcatc atgatcattt
      481 tcttcgtgga agaaaatcga ccagatgtta ttttgtaaag atatattcca gccgctgttg
      541 gttggccgga gttgtttttg ccgtcccaga caataatatg ttcgccgggc gacaattttt
      601 gctcattaac cagggctctg actagttgtc ccctggtgtt atagattgca acagttgttg
      661 gactgttgtc aatctggtta aacttgacat tagtactacc ctgaaacgga ttggggtagc
      721 aggcaacttc agggctaggg gtgaccgggg tgacaggatc actgttaacc acagtggtat
      781 cagtaaaggt ggcaattgaa ccatcgtcgc cgcaagtgat accgcgattg cccttaaagt
      841 caaaaccatg aacaataata ttgcccaaac tcattacttc ttgccagcca ttgttaattt
      901 tcaagatatg gataattcca ttgtaaccag aaacgacgta taaagtatta tttgtccatg
      961 acaatatagt gagggtacca ccattaggtg ggtttaaaaa taaactccaa gttaccccac
     1021 catcaattga gcgatataat tctctcctgt tggtaattga tgcgtagaac gtgcttggtg
     1081 aagctatttt cataaaaaat gaaaaatcac ttctatccac aaatgacaac tgccaagttt
     1141 gtccagcgtc ttgcgaaaca taacaattag atagatcatt gacgttatag cgtatcgccg
     1201 acaaaataat ggtttctccg gctacgtcaa agcctctaaa accaccctcg ccagcttcaa
     1261 cttgatttgc cttgatagta gtccaggtat tgccgccgtt gatggtcttt tttaggtcac
     1321 ctgaagacag aatataccca atctctggtg aagtaaattt gattcttagt aaactgctgg
     1381 agttgtttct aattccagga cagttggaca tatctgtcca agtattgcca ccgtctatag
     1441 ttttatacag accaaagctg tccccgcaaa tccaaccgac caggtcatca atcatccata
     1501 ggccgttaac gcagaatggt tggtcggggt gtgatacttc tactgattgc cagttttgac
     1561 cggcgtcggt agacttcata aaacggaaaa ccatggtgtt ggtccaacca gccacaaaat
     1621 agttattttg gtccaagatt tggcaggcct ccaaattgga gttgtgccaa gtgatatttt
     1681 gggcggcggc ttggccgccc agggtgatga taaacaccac caaatagatt aaagcgacct
     1741 tcttcatggc cacctccctt ttatttatat ttaattgtta aagatcaaac tcttattata
     1801 gataataata ccacaacaac caggataagt caatatttta ctttactggc cagagttggc
     1861 ggttttttaa gtttatggta taataaaaac aattagcaaa aatattatga aaaagatttt
     1921 aataattttt gtttatgctg gtcttggact agtgagtgga attctagctt ccgctttatg
     1981 ggcccaggct gccccagtta ttttttatga gtctaaatta agaataccta acgtgactaa
     2041 tatgactgcc accaccagca ttgttacggt tcaatattta gagaatttaa ttaatgtgat
     2101 gcttaggcct acttacctaa atccataatt ggttaatttt atgtccaaga aaacaggttt
     2161 attaatttgt ttagtgattt ttttatcagt cattgcctat ggcctagtgc aggctggtgt
     2221 tgttaacttt gttgacggta ttaatataca atatgaaaca atctataaag atcaagttcc
     2281 ttctaaggcc gaggcggaga atttattaat gccgttatct agtagttttt tccctagcgc
     2341 tcatttagaa aagaacgctg gcggttgccg tataaaatcg ggaactttct acattgatta
     2401 cacgcaggtg catttttggc aaggctatta ttgggggtct gccaccgcca tcgcaaccag
     2461 caccactgac aattcttgca tttgtccaac tggaacaact agaattttaa gaaggtcaca
     2521 agctaataat caaatcagcg aattggatac aacttggtgg aaagacttta ggtgggtcaa
     2581 tcgtggttta gggtttggta ttcaaggtga acttgtctgg actactcaat gcactaaatt
     2641 ttataacacc tgctgtccgg gaggttgtat gacaggtgat ccaggttgtt cttgtgccta
     2701 taacctcaat aattgcaaaa gtttattcag caacggccag ggtactggtt gcggtggcag
     2761 ccagcctata tattatactg aatatttata taactgtgtg gctactagcg agacattaaa
     2821 acaataatat gaagaaaaga ttaatacttg tttttgccac attttttatc ggtgcctttt
     2881 tctatgtaac tacccatggt gctaccaatg ttgtttcttt tcctgatggc gctaaaaatt
     2941 tgcagactat tactgctaac acagatgcca ttatcaagaa ctgggctgat acacttatca
     3001 tcggttcttt agaaaagaac gctggcggtt gccgtataaa atcgggaact ttctacattg
     3061 attacacgca ggtgcatttt tggcaaggct attattgggg gtctgccacc gccatcgcaa
     3121 ccagcaccac tgacaattct tgcatttgtc caactggaac aactagaatt ttaagaaggt
     3181 cacaagctaa taatcaaatc agcgaattgg atacaacttg gtggaaagac tttaggtggg
     3241 tcaatcgtgg tttagggttt ggtattcaag gtgaacttgt ctggactact caatgcacta
     3301 aattttataa cacctgctgt ccgggaggtt gtatgacagg tgatccaggt tgttcttgtg
     3361 cctataacct caataattgc aaaagtttat tcagcaacgg ccagggtact ggttgcggtg
     3421 gcagccagcc tatatattat actgaatatt tatataactg tgtggctact agcgagacat
     3481 taaaacaata atatgaagaa aagattaata cttagtggag ccaccgtctt actcttaatt
     3541 attttagcat caattttttt tattttggcc aaaaaatata gtccgctcat ttctcatcag
     3601 ccattaaata ataatcaaaa taatcaatta gaaacaccta acattgaatc aattgggagc
     3661 attaacgttg acaaccagaa ttttgatatt aagcttaatg ccatcaaaga tggtattgtt
     3721 gtggctgaaa atggccaacc gacgcgtaat gattctgagc caaaatcacc aggaacaccg
     3781 atggttagcc tagatattaa tggggctgaa ggaattaata ttcctaatga cgttattaaa
     3841 atatcgatta ctaaagattc aatcagcccc aaaccgatta ttatatcggc cggtaagcca
     3901 accactttta tggttgaatc agccgatgaa tggtatcatg ttttacgctt tgccgataac
     3961 agtcttaagg ctttggctgt cggggttgct ggccatgaaa ccaggctgat ccgctttaat
     4021 gctccaacta ccacgggcga ttacattatt tatgacgaaa ttttcggagt tgatggctta
     4081 gtgactgtta aataatattt atggtcgcta aaatagtcaa accattgcgt attaatatca
     4141 ccccaccatt gtcaaaacgg tgaccattct ctttctaccc tatttggctt ttttaattaa
     4201 agagatgtta actgtagttt ttgtcgcctt tattttgcca acacttctga tccgtgggtt
     4261 gatttcctgc agcgccgtaa atggccgcga attaagtatt accatgattt atgttatggt
     4321 gccgattctg gttaacgaag cgactgataa aatcccgtag atataaaatg cttcaagact
     4381 taaccattat taagctcgcg gccgtgttag ttttaagact ttgattcctt aagatttgaa
     4441 acaaaaggtg tatgattcaa cagaattgct aaaaaatgga atgcttcact aataaaatga
     4501 cgagcgaagg cgccaatcag aaagttccgc ggagagttga gttaagttca gaacaaaaaa
     4561 ttattagcaa caccatgtct gttatcccca ataatgacgg cgaagcgcat ttggtagaag
     4621 aaattaaaaa atcagagaat ttgatattag ggcacaattg aaagacgatt acgacaaagt
     4681 ttctagaaga aatgaaagct gtacaatacc agggagatgt tgtcattcta tcttccaggg
     4741 aggacgataa taattccatt gcttacgaca aaataatttt tgataacctg gaaagggttc
     4801 cgcaaatact tgatttgcgt taaaataata aaggggagat tgtatttata tatttctctg
     4861 gaccatctga aatagtgcga aaattaaaac aggaatatag tccttctttc gagggcagtg
     4921 aaaaaaacag aaaaagtgag cgattttact ggttggagta agccaaccca aaaagttcgc
     4981 gcattttcac acaaagagta taaatattac atagtttgct ataattatct tatgatcccc
     5041 aataatcaag ccctacccta ccctagtggt tttttattaa tagataaacc cagtggacca
     5101 acatctcacg atattattaa taacttgcgt catcttactg gtattcaaac aattggccat
     5161 gccggcacat tagatcctct cgcttcaggc ctcttgattg ttgctatcgg ccgaacggcc
     5221 accaaacaaa tccctaatct agtaaaacaa gataaagttt ataaggccat tatctgtctg
     5281 ggtggattaa gtgatactga tgactctcaa ggaaaaatcc aaccatttac aaattgtcat
     5341 cagccaacta aacaagaaat aaagataatt ctcaattctt tccgtggtga gcaaaatcaa
     5401 ataccgccaa tgtatagtgc caaaaaaatt cacggagaga gactatataa attagcaaga
     5461 aaaggacaag tcgttcagcg tcagccaaac ttaattacta tttataatat caatttactt
     5521 cattatgaat ggccaaccct tagcctagaa atccattgtt cagctggtac ctatattaga
     5581 gctattgccc gtgatttagg aaaaatgtta ggttgtggcg gttatttgtg ccaattacga
     5641 cgtactaaaa ttggcaatta ctctatccag caggcagtaa ctttagaaaa attgacctcc
     5701 cataattgga caaaattttt attcaatctc taacatctat tccacggtta cggatttagc
     5761 taggtttctg ggtctatcta cgtccagtcc tttagcatcg gcgatataat aagctaaaag
     5821 ttgcaaagga attacattaa taatgggtga aagccaaaca tggactaccg gaaccttaat
     5881 aaggtaatcg gctaatttct tcactggctc ataatcttga tccacaatgg cgataacttg
     5941 tccgcctcgg gccttaattt cttgaatatt attaagagtt ttatcactca aatcattttt
     6001 agtagccaag acgactactg gcatattttc atcaactaaa gcaataggcc catgtttaat
     6061 ctcggctgct ggataccctt cagcatgaat atatgatatt tcttttaatt taagagctcc
     6121 ttctaaggct accgggaaat taaggccacg gcctagatac aaaaaattct caaaattcat
     6181 aaaacgttgg gctagctcta ttacctggct attggcctca agagtcttct ctgccacggt
     6241 tggcaaatgt cgtaattcat ttaaaatttt tttatctatt tcataacctg cttcatggcg
     6301 ccaataaagg gctaaaagca ataaggcagt aatttgggca gtaaaagctt tagtactagc
     6361 aactccaatt tctgggccag catgtaaata aatacctgaa tccacttctc tagctactgt
     6421 tgacccaact acattaacaa tccccaaaga ttgagcgccg cgacttcttg cttcacgtaa
     6481 agcagctaaa gtatcggccg tttcacctga ttgagagata gcaataaata aatcatggct
     6541 acttaccagg gggtgacggt aacgaaattc agaagcataa tctacttcta ctggcagacc
     6601 gacaatctct tctaataagt atttgccaat taaggctgaa tgccaactgg taccacaggc
     6661 agcaataata atgcgctgta taacctcacg atgccaattt aatgaaagtt taatttggtt
     6721 atcagttatt ctaccggcta acacattagc tagactctgt ggctgctcaa aaatttcttt
     6781 gagcataaag tgcttatagc ctttcttttc aatttgctcc atacttaatt tcacttcttc
     6841 tacctgacga tctaataact taccagctaa attcttaatc tggtatccct tactatcaat
     6901 aatagctaaa tcaccatcct gtagataaac aacatcttta gtatgattta agatagctgg
     6961 cacgtcggag gcaataaaga tttcgtcctg gccaactcca ataatgagtg gcgagctacg
     7021 cctagcagct actaatttat tttcactagc gtgaagtgcc accaaaccat aagaaccttc
     7081 tactaaagcc aaagctttag ttaccgcctc ctctaatgag cctttataaa acttttcaat
     7141 taaatgagcc aatacctctg aatcggtttc tgaactaaaa atatgacctt cactaattaa
     7201 taattctttt agggcttgat aattttcaat aataccatta tgcactatgg caatttggtg
     7261 atggcaatca aaatgaggat gagcattaac ttcgctagga ataccatggg tagcccaacg
     7321 agtatgggca atccctacgt gtccccccag atcttggcga gctaccgcct cttctaagtg
     7381 agctaccggg cctacccgtt tatagcatac taaattatta ttttccacta agcataaacc
     7441 tgcactatca taaccacgat attctaatct tttcagaccc tctattaaaa tctctgaagc
     7501 acgatgctgg ccacaatagc caataatccc acacatattt taaattaaaa aatcaacaat
     7561 aagtaaggct acaatattta caatcttaat cattggatta atagctggac cggctgtatc
     7621 cttatatgga tcacctaccg tatcaccagt aactgctgcc ttatgagcct cggaaccctt
     7681 actaccacaa ttgccactct caatatattt tttagcgtta tcccaagctc caccacctga
     7741 agtcatggaa attgccacaa aaataccagt aacaatagaa ccaattaata aaccacccaa
     7801 cgctggcaca ccaaaaatga aaccgaccac gattggagcg agtatgggta ttaacgaagg
     7861 tataatcatt gctttaatag cagctttagt aactatatcc acagtgcgac cataatctgg
     7921 tttctcagta ttagttatta tgcctggctt ctctttatat tgcattcgta catcatcaac
     7981 taccgaacct gcagttttac caacagcctc catagctaaa gcaccaaagt aatagggtaa
     8041 taatccacca ataaataaac caataaggac ataaggattg tctatcaaaa attgactgcc
     8101 ttgagttttt tgagagaaat cagcaaatag aactagagaa gctagggcag ctgaaccaat
     8161 agcataaccc ttagtaactg ccttggtagt attgccaaca gcatcaagag aatcagtaac
     8221 attacgtatt tcctcgccca atttagccat ttcagcaata ccaccagcat tatcggtaat
     8281 agggccatag gcatcaatag tgataataat tccagctaaa gataacatgg atactgcagc
     8341 aatagcaatg ccatataaac cagccagaca gtaagctccc aaaatagata gaacaacaat
     8401 aataattggt ccggcagttg atttcataga aacagctaaa ccaacaataa tattagtccc
     8461 gtggccagct ttagaagcct cagcaatttg tttaactggg ccatgcttag tggaggtaaa
     8521 ataatcagta accacaacaa aaaacccagt agtcactaaa ccaataacgg cagctaaata
     8581 taaattattg atcgtaaata aaccgttatc tttggccgcc cagacaataa taggatagaa
     8641 aatgattgcg gcaattaccg cagtaataac taatcctcta taaagcgcgg ccataatgtt
     8701 taatgatttg cctaagcgga taaaataaac accaataata gaagcaagga tggaaactgc
     8761 tcccaataaa agagggagaa taattagcaa ttgattatct gtaccaaaga tcaatccacc
     8821 caatatcatc gccgcgatag aagtaacggc gtaagtttca aataaatcag cggccattcc
     8881 ggcacaatca ccaacattat caccaacatt atcagcaata actgctggat tacgtggatc
     8941 atcttcgggg ataccagcct caattttacc caccaaatca gcgcccacgt cagccgcttt
     9001 agtaaaaata cctccgccta aacgggcaaa gatagaaatt aatgagccac caaaagcaaa
     9061 acccactaaa gcatgaatat cgtgagtgat aaaataaaaa ctggccaaac ctaacaaagc
     9121 caaacccaca accaacattc ccgtaatact gccacccctt acagctacgg ccaaagctgt
     9181 tttttctcct gatttagctg cctcagtagt agtaatatta gcccgcacag aaatattcat
     9241 gccaatatac cccgctaagg cagacaaaat agcaccaata ataaaagcta gagccgtaag
     9301 ccaacctaga cccggcagta aaccaataat aaaaaataaa aggatggcaa ctagccctac
     9361 cgttcgatat tgacgattta aaaaggcccg agcaccagct tgaatagctg aagcaatact
     9421 ttgcattttt tcatcaccac gtggtaattt taaaatagaa taaatggcaa taatcccata
     9481 tattatggcc aataaagcac tcgcaataat tacaatgata gcaatagaca taatctttat
     9541 aaattaataa tcccaatcaa taaccaataa gctcaattat tggtcaaatg ggtagccact
     9601 gggaattgaa cccagactag cggagccaca atccgctgtt ctaccattaa actatggcca
     9661 ccatcttctt tataatttat agattctaag acaattagtc aatcctaacc taatttcttc
     9721 tattggttca tagcctggca actcaaactg attactaatt aaaattgccc cgggtttagc
     9781 ttctttcttt aatttaagag ataaccgagc catcattggc ccgtttaaat aacagtaaaa
     9841 acaatctaca tctttcaggg acatctttaa aaaattcttt ctaattattt taattttact
     9901 gccaacaaga aataattgta attttgctaa gagataaacc gaaaaaagca actcaatacc
     9961 caccaattta tatttctctt tatcctcaat ggtcttttct aggtgtcgca gaaatccagc
    10021 ccgaccacaa cctaactcat aaactttagc accagctggc aaatctaaag agataaactg
    10081 ctgtaccgcc tcatctaaaa ctttactcct actactaaca agaggggcat agcccttaaa
    10141 aataagatta aagaactctt ctaccaaaaa agtcaataat actaaaatta gtaataaacc
    10201 taaaacctgt aaccaaatca tatttttaaa ataataaata acaaagtacg cccagaggga
    10261 atcgaacccc cataaccagc ttagaaggct ggtgttctat ccattgaact atgagcgcta
    10321 atcaaaggct aatgtaacaa aacaataagt ctttgtcaat taatgtaaaa gaataatttg
    10381 cctggtgatt tctgtctgtc ccccatattg cttaatggct ttaatattta agtaatttgt
    10441 acctaaatgc aaattaatgg gccgattaaa ataacccgac tcatcactaa taattatttc
    10501 accattaaca tctatctgaa cttcaggatc agtttgccca cttaataaaa tttgtcgctc
    10561 agtggtagct atattgtctt gagggctaat tatcttaagt ctggggggag aaaaaatttt
    10621 ttttaaagaa aaaattatat aaataacaat tgtaataata agtaaggcca ctagaatatt
    10681 gcgaataatc cgtgggaatg accacagatc tttcttgcga actaactgtt ggccgaaaaa
    10741 attattcatg gatatttaat gtctaattct actactcttt tttaatagtt tggtctaagg
    10801 tatttcttgc ccaaggtgca acattaacaa cattaagaac aaaattaata attatataac
    10861 tacaaaaaat aaccactaag cctatcaacg cattaataag cgtctgctga ccttttttaa
    10921 aattctcact attgccagca gcaataatca tctgaatacc accaacaatg aacataacta
    10981 aagcagcact gccagctaat cctaaaatga ccttaccaac aaaaatagcc atttttgtaa
    11041 aatcttgaat tgtatagtcc ccttcctcat aatgctctgt gggtactaac tgctttgtat
    11101 tctctgcagc cgcaaaagat atggttggcc aagccaacat cataattgac aatactaaaa
    11161 atataaattt cgtaaatttc atataatttt tattctggtg gtggtaataa tgtttgactt
    11221 aatttactgg ctcctaaatt ggcaatagcc tgtaactggt caggattatc aactacgtta
    11281 tcaatcattt cagccataat cttctgggca gatttagcat catagccttt ataccaactt
    11341 cgagctgtta ataattggct agcaaaaacg tctaaatatt cttcagataa ttgctgctca
    11401 attaaactac gtagggctgt tggccgctta gttttatcca aatagctagt aacattttca
    11461 gccctagtaa taaactgaat aaaatcccaa gcttcattaa tatgttgact ctttttagaa
    11521 accgtctcta cccaataatt agcaatatta actggctgag tagtactagc taattgaggt
    11581 aatttactaa caccaatatt taaatcttgt aaatagtctg tggcattact cccattaaaa
    11641 tcagaacgcc gaccagctac aatcaggggc aaatcgtaac tatagccaaa cataatagct
    11701 gcctgtcccc taataaacat atccaaagaa ttatcaaagg ccttatccca acaatagact
    11761 tctttattaa tattagcaaa atcactataa aaacgtaatg cctctagccc tggatagcga
    11821 tcgccaaact ttttattaaa aacaacctga ccattttgaa taactggtac ctgactttgt
    11881 aacattaaag ctgccagaat atccgctgac ctcttcacat tagcgccact gcctatagct
    11941 acccctgctt gaacaatatt accttcaata tctttctctg ttaatttctt taccacttct
    12001 tgaaagtttt tatcccagta agctgggggg ttaataatac ccttattatt aaataaatcc
    12061 cgacgataaa acattgctaa agtatctacg gatagaggta ggccgtaaat cttattgtcc
    12121 ataaccacgt cactggctac aacgtcaaca aatcgagctc gcaaatcttt aagagaaaga
    12181 ctgggagtag tttttagctc atatattacc tcctttttaa tagtcccttt ctgaatggga
    12241 taaaccatgc ttatactagg aggcattggg gcaattttat ttttatactc aggtaaccag
    12301 gtactttcaa tagaaaaaat atccggacca cgatcttcag ctaaagcatt aataagctct
    12361 ttttcatatt cctcataacg taattttcga tagttaatag taatgttagg atgcagtgct
    12421 tgatatttag ctaaaatttc ctgaaaatca tccggctcat cccaggcccg ccaataattt
    12481 agagtgattg gttgcattgc ctctttaact tcatgtgact gaagacggca accaaaacca
    12541 ctagtgacaa aaaaaatagc tagtaaagac agtagactaa tttttttcca cataattaac
    12601 tgcttaaaaa taatcctata ttagttatta taccgcaaaa aaaggaaatc agccaataga
    12661 ctgatctcct ttttaaataa tgatataaaa ttactggcaa ttacctcctg aacttcctga
    12721 accactacta ttatctgctg taaaaccagc agccggggca ctactagata gttgagccga
    12781 acactcctct ggtgcattat taaaagccga acaaatggtt ttcagcagac tagcactgtc
    12841 tcgattacta cttacctcct gtccattaat aattaaagtt ggtgagcctt gaatattata
    12901 tttatcattg tcagctttat cgacattaaa tggcgggaat tgactattcc atgaattttt
    12961 atcattaaaa ctactcataa tattatattg cttatccaca gaactagtac aagtacccaa
    13021 cgcctgttca tcaatctttg cctccttata gcaactggcg gcatcgccac ttttgatgaa
    13081 acaatttaaa taactaagga atttatctgg ctgttctttc tgaatacaat actgagttaa
    13141 attttcttca atttctggct tatcatgcat agcataatcg cagaatctta ctttgaaatc
    13201 aattttatcc cccaattttt ccactaccgg caaaataccc cgctccattt gtaaaccata
    13261 aggacaataa ctcataacaa agaggtctac ggtgggttta tcacttttct gctcgacagt
    13321 agctataccg ccattctcat tactactagt agctgctgcc aactgttcat taatagcatc
    13381 aatatctaaa gcctgtggaa aaaataattt gccatcaaga cttaagtagg attcaattgg
    13441 ctctccactg cctacatcaa cttgcatttt ataaaatccg tccatcttct caatttcctt
    13501 aactatagcc tgacttccag gagccattaa gacagcatta ataaaggttt ctgccttttc
    13561 tttggcttca tctggtttta aggtcttcgg ccgagccgag caaccagtaa gcactaaaac
    13621 catggccaaa ctaattaaca atagtttctt cataaaaata atttaaagat ttaaagaatt
    13681 aataatataa tactataaaa aatataaaag ttaaagccaa gtaacaccaa ctacctggtc
    13741 acccccctcc ttaaagcgca ttagatgaac cccccgagta tcacgacctg attccttaac
    13801 tgaggcaaat ggaatacgaa ttacctgacc attcatcgac attactatta aatctttatt
    13861 agccataacc tttttatcaa ctacgaaagc attgactatt tctcctgttt catcagttat
    13921 cttggctgtc ttgataccaa ggcccccacg attctgaatt ttatattgag ataaattagt
    13981 tcttttacca taaccatgag ctgtaactga taatatttga tagtctttta ttttttctgg
    14041 atcagtgtta ataaccccca taccaataat atgatctcct ttcctcaacc gcattccaat
    14101 aactccactt gctccgcgcc ccatgtcacg aacatctgtt tccttaaaac gaatagcttg
    14161 gcctagagca gttatcatct gaatctgatc gctaccgcta gttgatttga cccaaattaa
    14221 ttggtcatct ggctttagtt taatagctag taaaccagta cgccgtatat tggcaaaagc
    14281 atcaacacta acccttttta ctaacccctg acgagtagca aaaaacaaat actctttatc
    14341 tcccatttta tctaaaggca gaatagctgt aattctctca tcactagcta attgtaaaaa
    14401 attaacaatg gcctgaccct tagctgtccg tgaagctagt ggcaactcat aagctttaag
    14461 ttcaaacatt cgtccccggc tcgtaaaaaa caacaagtct ccatgagcca tggttgtaaa
    14521 taaaaattgt accgcatctg cctccttagt agttaatcct agtacacctt taccgcctcg
    14581 gccctgaacc ttaaaagtat taccaggcaa acgtttaata tagccatctc gggtcataat
    14641 aactaccact tcttcattgg gaattaaatc ttccgcccta aaatccttaa tatcttgatt
    14701 cattatagta gttcgacgat tatcaccata tttctcggcc atatcccgac attcttgttt
    14761 aataatattt gacactaaag actcagaaga aagaatctgt tctaaatcag caatggcttt
    14821 atgtttttct tttaactctg tttctatctt taattgctct aagttagcta aagtttgaag
    14881 cttcatctct aaaatggcaa ctgcctgctt ttccgataat ttaaagtgtt tcattaaatt
    14941 caccctagcc cggtccttgt ccttagaggc tttaatcatt gcaataactt catcaatccg
    15001 cgctaaagcc atcataagac cttctaagat atgagccctt tctttagcct taattaaatc
    15061 ataggtagta cgccggcgaa taatttcctg gcgatgtttt acaaactcct ctaaaatggt
    15121 ttttaaattt aaaatgcgtg gctgaagacc attaactaag gccaacatgt taacatgaaa
    15181 agtttcctga agaggtgtct gtttatataa agcattaaga atttttttag gataactatc
    15241 ccgttttaaa tcaagaacta ttcggatacc ttctttatct gactcatccc gtaaatcttt
    15301 aacgccctca atctttttat ctttaattaa atcagcaatt ctttctacta aaacagcttt
    15361 atttacctga tagggaattt cactaataac tatctgaaaa ttaccagcac tattctcaac
    15421 aatttcagct cgtccacgca ttacaatgcc acctttacca gtagtataag ctcgtaaaat
    15481 atcttcatga ttatagataa aaccacctgt aggaaaatct ggccccttga tgcactccat
    15541 taattcttca atggtagctt caggatggtc aatcaacttt tcaatagctg ctgctacttc
    15601 tcgtaaatta tgaggtggaa tattggtagc catccctaca gcaataccca tacaaccatt
    15661 aagcaacaaa ttaggaattt tagctggtaa tacttgaggc tctgtcagtg agccatcaaa
    15721 attaggaatc atctctactg tatctttatc taaatcaatt aacatttcct cggcaatagc
    15781 cgctaattta gcttcagtat aacgcatcgc tgccggtcga tcaccatcca ctgaaccaaa
    15841 attaccctgc cctttcacta aaggataacg catagcaaaa tcctgagcca aacgcgccat
    15901 agcatcataa accgcactat cgccatgagg atgatattta cctaaaactt cgccaaccac
    15961 agttgctgac ttacgaaatt taccgccaga atgtaagcct aacaaccaca tggcatataa
    16021 aatacgacga tggactggct tcaagccatc tcggacatcc ggcaaggctc gtgacacaat
    16081 cacgctcatg gcgtaatcaa gataagactg acgcatttca tctactatgg gctgcactaa
    16141 aatagaacca gaaggtacag ctgaacctgt ttcttccttc aattttttta cctcatttaa
    16201 tttattatca gccgagttat ctaccacaac tgatgatttt ttaacagtca taatatttta
    16261 ttatttatca ggtaattgct ctaatttctg acgtaattca tttaatttaa ttttgttttg
    16321 ttcttctaat aatttctcct cgggggttaa atcttgcgtg gaagtattaa tctttttaag
    16381 agcctgagtg ctacttttaa cttcttctat cccactactt aagtcagact gaatttgtga
    16441 aatatttagt agggggcggg cattagactc attattactc attattaaag aagagcgtaa
    16501 accataaaac caaaaaatta acaaaattaa catgattata gtcaccccca accataatat
    16561 taagtatcgc gtttctggtt tccagtcacg aggattaaat ctagcatttt ttgagctgtt
    16621 tatttttttc attgatttat ttaaactgat ggtgataaaa ccactaattg aagattctcg
    16681 ttatccaaat cgcggcggct aatcttcaat cttgactcat tggttggtga taaaccaatg
    16741 gtcttaataa aattttctag actatccagt tcaaacttta aacctggtag ttgataatgc
    16801 ataggaataa ccattttagg gtcaacttga tttataactt ctaccgcttg cttgccatca
    16861 attgtataat gaccaccaac aggaacaatc aaaatatcag taccagatat tctttctaac
    16921 tggtctggtc ctaataaatg ccccaagtca ccaagatgag tgatcgttaa atcactaaac
    16981 tctaatctaa aaattaaatt atgaccccgt tctttacctt tctgagcatc atgatagcta
    17041 tcaatagcat cgatacatac tccgccaatc tcgtactctc ctgcagtatc tattatatac
    17101 gaccagccct gaacaccctg aataaaatta tgatcatgat gtccatgagt aatggcgaca
    17161 atattagctg atagattatt tggtaacttt aaaccagtat aattaggatc aaaaggatca
    17221 atgactaaag ctatttcttg accattaatt ttatcttgga acttaaagca agattgtcct
    17281 aaccaattta taatcataaa aaataggtat aattataagg aaacccgtca ccgggctaag
    17341 tacaatttag caaaaataaa gccgtctgtc aaagctataa gactgattta atcagtaaaa
    17401 ttattaaacc tgacgaaatt ggcaaatgga cctaaaatca caataactac ataattgact
    17461 aggtctggcg ggaaataatc cttgtttgat ggctttaata gtttcggcta cttcatgttg
    17521 aactttgtct acatccttag ccttagcctc aaaacttaca aaactaccat tattaagata
    17581 acaaaaagtt aattttccca ccggccagtt aaatacttct tctactgcca ccttataaag
    17641 tattaactgt cgttttgttt tataatctag gctctcttta ggctggcctg ttttgtaatc
    17701 gatgatctct actgtgccat cgggtagctg atcaatacga tcaatggtgc cttttaaaat
    17761 aaaaccttct cctttccaag taaattgttt ctctagccca agtactttag gccagccctc
    17821 atcttgaact ttctgccaaa ttaaacgcag gctctgctca ccaagtttac ggtattgatc
    17881 ccgttcttcg gctgaattat aaccctcatc aacccaattt cttttataaa tatcaagcca
    17941 atccggccaa tctaaatact caggggtagc tctctgtcca aaaagatcaa cccctccaga
    18001 agatttaagg caaggtaaaa atatctcata aagagagcgg tgcatcaatc ggccataaat
    18061 tgtattagct ttctcgggaa ccggtacttt taaaagaaaa ttaaatttat attgcaaagg
    18121 gcaattacta tagccctcga actggctaaa agaaaattta ttacctggca atggttgctc
    18181 ggtcttgact aactctgggg ttggcttgga gagtgactcg acttcacgaa ctagctcaaa
    18241 cacttgggta gaatctagtt gacgttcagt gatgttgtta gttaaatcta gttctcgcaa
    18301 aaaaatagat ggctgacggt ctctcgctcc gccatagtca cgagcgtaag ctaaaataag
    18361 tttttcttta gctctagtca tggccacata aaatagacgt cgctcttcgg ctaaatgcat
    18421 atcactagta ccaactggct cagcaataag agcagcgggg ataggtaaac tgtctccttg
    18481 ttttatggta ggaaattttt tatccactaa atcaggtaaa atgacatacc taaattctag
    18541 tcccttggct gagtgaatgg tcatgatttt aaccacatcc tcatcttcta gcggtaaatt
    18601 taagctgcca gtttcgcccg cctctagttc caaattaatt aaagtaataa aatcttttaa
    18661 ccgtaaatcc tgaccaccat tttcaaaatc ttttatttta cgataaaatt gatttaaata
    18721 gtgaaaaatc tgccaatctt tgtctggatc aagaccagat aatagaccag atttatacac
    18781 aaaatcaaca aatagacgac tcggtgcttc tttggccacc atcaatgatt gctcatcaat
    18841 aaggcgtaaa agtttagcca taatttctcg gccagcagga ctaattttaa aagccaaatc
    18901 attatgcttc atcgcctcaa atagcgacca agatttccga tgagctaggg cagtaagttc
    18961 tattaaatca acggcaggta aagcaaaagg tggtgaattt aacacccgat agagagccgt
    19021 ggattcatga taattatcta agagcttaaa ataggccaaa caatctaaaa taattggctt
    19081 ataatatagc ccccgcatgg acataaactg acaaggaatt tgacgtctca tcaattcatt
    19141 aataaattta tcagcatcac gattggaacg aattaaaata gcaaaatcag accattggac
    19201 ctcgtcgcta gtttgatgtt cttctttaat ataatcagca attgctacta cctcttctga
    19261 ctcggtatga aatggccaca cgtcgaccga ggatttaggt atacccaccg ctactaattt
    19321 tttgtttagc gataatttaa cctccaaacg gtcaggatta ttatactgaa tagaacgata
    19381 ggcttgatcc aaaatagcct gtcctgaccg ataattagcg gttaggacaa tttctcgggc
    19441 ctcaggaaaa tcatctttaa attttaaaat attagctaag gaggctccgc gaaatttata
    19501 aatactttga tcatcatcgc ccaccacggt taaattatta gctggcgcag ccaataattt
    19561 caaaagctca tattgagccc aattagtatc ttgaaattca tcaactaaaa taaacttaaa
    19621 ctgctgtcga tattgttcta aaatattagg tcgttcccga aaaagcttta gcgtataaac
    19681 aatcaaatca ccaaagtcaa ggcaagaatt atctaaaagc agttgattat atgtagcata
    19741 agcctcggcc aattccctaa gtctagtaac ctccagctta gctggttcat tcatatcact
    19801 taaatctaga gactcaacat aatctaaata ctgctgggac gaaatatctt catctttaag
    19861 atgactaaaa tgcttcacga ggtcagttag aaacttagtc tcattgccca gcggccggta
    19921 ataattaagc atcaggcgat ctaaattttg acgcagtaaa acccactggg aagtttcatt
    19981 aagcaattta aaattaatag gaagaccaat atctaaacca tgttgccgta gaagacgttc
    20041 accaaaacta tggaaagtct gaatccataa atctgtataa ccataaggca ggagcctatc
    20101 tgccctctct tccatttctc cagcagcttt atcggtaaaa gtagtcagta agatttcttc
    20161 tggtttagct tgccctgaca taattaaata aagcaaccga ttaactagca cggtagtttt
    20221 accagtgccc gcaccagcaa caataaggag cggtcctgtg ccatgttcta ccgcccgtcg
    20281 ctgctcctgg tttaaattag ccaaatagtc atcaagctgg gtcatactaa tctagggtca
    20341 ataaagaaac taatttattc tggttagcta ctggcccaat aatagctagg ttaagacctt
    20401 tatccacaaa aatctgtctg gctacttctc gaatgtccgt tgcactgact gaatttaatt
    20461 tagtaagata gtcttggggt gtatctagaa tagcctggtt atcaccctgc cctaacctta
    20521 aagcctgttg ataaccatac caccttgcta agtcatctga gccttccagt cccagtatta
    20581 gccgaccgct caaaaatttc tttactcggt ttagctcacc ttcttctact aattcagtgg
    20641 ttaatttttt atattcctga agaatcatgg aaatagctaa aggtaaagat tcttttttga
    20701 ctccagcttc agttactaaa taaccactat ctgtataagt ctccgcctca gtgcggacat
    20761 aataggctag acctcgacgc tctcgaatct ctaaaaataa tctagagctc attgtgcccc
    20821 ctaaaatagc agctaataac ttagtaggta gttctgccgg atggccataa ggaaaagctg
    20881 ggacacctag ggctagatgc acttggtcag tggttttatt ttctaccaat aattgaggct
    20941 ccgtttgttg gtttataaca ggtagtttat ctctaaaatt aaatgtagta ggccattgac
    21001 taaaatattt attcactaga gtatcaacat caggtgatag attaccggct aaacaaataa
    21061 tagcacgaga atggccataa tgagtctgcc aataatctat taggttttct cgctttaagg
    21121 aattcactgt ctccttagag ccagcagtat cccaacccgc aggggtatca ccataaagaa
    21181 ctcgctcaaa tacatcactt aagtgcatta atgggttgtc ttcatacata ttaatttcct
    21241 caatgataac tcctcgttct ttatctattt cttcggatgc aaataaagaa tttaataaca
    21301 tatcacttaa gacatcaaga gccacctcgg cttggttggc tacacactta acataaaaag
    21361 cagtagattc tttactagta aaagcattat attctccgcc aatagcatca agctcactgg
    21421 atatagctag agtggtgggt cgcttagttg tacccttaaa aaacatatgc tctaaaaaat
    21481 gtgatactcc tttctcttga gcggtctcaa attttgaccc ggtagccaca attactaaaa
    21541 tagtaaaagt ctgacaatta tctagtggca ctttaagaac taaaggaaaa ttatcattaa
    21601 attgtctttg ccacatatta ttaattcaat tttgaagata aataagctat aagctcatta
    21661 acattaatac tctcctgctt catcgaatca cgatctcgaa cggtcactac attattagct
    21721 aaagtatcaa aatctatggt gatagagtag ggagtgccaa tttcatcctg ccgccgatag
    21781 cgtttaccaa tattaccatt atcatcaaac tcacagcgcc aatgcacttt tagttgatca
    21841 aaaatctccc tagctttagc gactagttca ggacgattac gtaaaagcgg aaaaacagct
    21901 actttaattg gagcaagtgc tggttttaaa tgtaatacta ttcttttact gccatcaggt
    21961 aaattttctt ctccataagc ctcggccaaa agcatcaaga atacccgccc tacccccgaa
    22021 gaagcctcta caatatgagg aataaaggtc tcatgagata ttgggtcgta ataatccaat
    22081 ttaacacctg aatatttctg atgctgactt aaatcataat caccccgcgc atggatacct
    22141 tcaatttctt gaaaaccaaa aggaaattta tattcaatat caaaagcggc tttagcataa
    22201 aaggctaatt tctcatgctt atgccactgt aaattgttgg tactaatacc taaattattg
    22261 atataataat tccaccgctg ttctttccat tggttaaatt cttccaggtc ttcggttggc
    22321 ttaacaaaat attggagttc catctgttca aattctctgg tacggaaaat aaattgccga
    22381 gcggtaattt cgttacgaaa agctttacca atttgagcta tgccaaaagg caattgaagc
    22441 ctagtactat ccatgatatt tttataattt acataaatac cctgacaagt ttcacctcgc
    22501 aaataaactg gctcggctcc agaaccggta aaatcaccaa gattagactc aaccaataaa
    22561 ttaaaacggc gtactgcagt aaaattttgc tgaccacaat cagggcaaac aactaaatga
    22621 cgatagtcat caaataattg attaaactcg ttctcggaca tagtttcatt ggctttaacc
    22681 ccggcttgag ccaaaagctc atcagcccga aaacggctat ggcaatgacg acactccact
    22741 aagggatcag aaaaattagc tatatggcca ctagcccgcc aaaccttggg atgcataaaa
    22801 atagcactat ctagaccgac aatattatca tggccgtaaa ctaaatcttt ccaccataat
    22861 tgcttaatat tattagctaa ctcaacgcca taggggccat agtcataaac cgccgctaag
    22921 cccccataaa tctccgacga ttgataaata aacccccgac gtttacataa gcttactaat
    22981 gaatctaaat cgatatctga cataaaatta tctttaataa agatagtcta ccattggaaa
    23041 ctagttttgg caattagaaa taaaaagccg ccgatttaca tcggcggctt tctcccctcc
    23101 ttattatcaa ccggttgatg tctttaaacg gccagctttc cgaaaactat aaaaactaca
    23161 acggctaaaa ataacattat ctaacatctc taaaccaaag gcctcacaaa tttttaataa
    23221 cttattaaat aaaattaaat ccacctcatt gggttcagcc ccgccggaca aatgattact
    23281 agcagtaatt aattgctggg ctggcaattt caaaattggg ttaaaaattt cacaaggact
    23341 aaagtcgggc tgacgcatct gagaaaagag aaaagattta cgtctcagct catgacaacg
    23401 atgatctaaa taaccaacta ccaaccgttc ttgggtacaa caccgcaaat atttaaactc
    23461 ttggtaaata acttcgtcac tatagtaaat attttttagt ctctggcgac aaaaacgtct
    23521 agtcatttct acagctgcta aaaaattatt agcctgctca ctatttaaac cggccttcac
    23581 caattcttca aaactcgctc tcactaaaca aggcgccggg atttttttaa gtactctctg
    23641 ggctaaagca atagaggatt tgatttcttg attttggcta ataataaagg ctaacagctc
    23701 gctattgcta aatttagcta gatcaaactt tagccaatta gccgcatccg gcaatttcgc
    23761 cgggatggga tagatagaca tgttagctct attaagaact aaaaataggg cggttaaaag
    23821 gcaaatagtt tgaccgtcat tatcatagca tttttattta accttcaaaa gatgatgccc
    23881 taaaaaagaa ctaaactctc tttttctcta atgaaatcaa gtaatatcca ttactgacga
    23941 taactcctac gtctaaagtt atccccatgc cgtggactag tcggccgtcg cctggatgaa
    24001 ccaagagacc tatcagctgg taaccctttt gccttatagc gctcccaaat ggcctcatta
    24061 gccggtaaat ccttcatggt caaattaatc cgtccttgat catcaatatc ttttattcta
    24121 acactaactc tgtcaccaat attaagcata tcagatggct tactgacata aaatggagct
    24181 agctcactag catgtaccat tccatcatga ttgggcaata attcaataaa ggcgccaaaa
    24241 tctaacaaac gaacaacttc accgacaaat tcttcaccca cggcaaattt atgggcaata
    24301 tttttaatcc attgcactgc tagatctaat ttatctttat tattaccaca cactgtaacc
    24361 tggccagttt gttcaatatc aatagtcaca tcacaggcgg caataatttc atttataatc
    24421 ttaccgccgg atccaataac atcacgaatc ttatcagggt caataaaaaa agtgacaatc
    24481 tttggagcat acggagataa ctcggctctt ggctcagcaa tcgcctgttt cataacttct
    24541 aacacctgca gacgagcctt acacccttga ttaatggccg tacgaataat ttcttcatta
    24601 agcccacagg ttttagtatc aagctgaata gcggtaattc cttcggaagt accggtaatc
    24661 ttaaaatcca tacctcctac gccatcttct aggtcctgta aatcagtaat aaccttccac
    24721 tggctcatat cttgattgga agctaatccc atagctatac cggctaccgg ccgtttaata
    24781 ggcactcctg catccattaa agataaagat gaggcacaag ttgcacccat agatgacgaa
    24841 ccattagagc ttaaagtctc acttactact ctaatagtat aaggaaactc ttctttactt
    24901 ggcaaaactg gcactaaggc cttctcagct aaagctccat gcccaatctc ccgacggcca
    24961 gcaactttaa tcggcttagc ctcaccagta caataaggag caaaattata atgatggata
    25021 tatctcttag tagatactcc ctctaggccc tctaaagact gctcatcact aggtgagccc
    25081 agtgtaacaa tggacattac ttgagtttct cctctcgaaa agagacccac cccatgatta
    25141 cggggcaaga tactaacatc agccaacatc ggacgaattt catctaacgc ccgaccatct
    25201 actcgccggt cagcctctaa aatattttta ctaacaataa cctcaatata aggatcaaca
    25261 atttgactaa taagtgcctg ccgtaaattt ttttctatgt tctggctaaa taaatattcg
    25321 tctaatttat ctttaatagt gtccacggcc aattttcgtt gacctttagt ataataaatt
    25381 ttatcaaata aaataggctc agcattgtgg gctaaccaat cttccgccat tttttgtgct
    25441 ttttcacgaa gctcctgagc ctgtaattcc tcagccgaaa gacgaatagg tttctcctgc
    25501 tttcttcctc ttggtccaac ccgatgataa agctcttcaa ttaaatcaat agaaccttga
    25561 agggcctgct ctccagctaa aatggctttg atcatttcct cttctggtac ttcttgcgct
    25621 cccgcctcaa tcataatcac ccgctcccta gtaccagcga caattaaatc aaggtcactt
    25681 tgctcccgtt ctttataagt agggttataa accaattctc cattaatccg gccaacccta
    25741 actccgccaa tgggtccctg ccaattaatg cctgaaattc ttaaagcagc tgaagcagca
    25801 attaaagcaa caatatcata gtcattggtc tgatctacag ataaaactgt caaaattacc
    25861 tgaacatcac gtcgtgagga ttcatcaaat aaaggcctaa tagcccggtc aaccattcga
    25921 ccattaagaa tagcttcatc ggtcggacga ccttctcgtt taacccaccg ggaaccttta
    25981 atgataccag cggcatatag cctttcttca aaatccacca ttaaaggaaa aaaatcaccg
    26041 tcttctcgcg cctctttagc ttctacaacg gtagctaaaa ctactgtatc gccatattgc
    26101 acggtcacgg ccgcgtcagc ctgacgggct aaacgaccag ttttaatagt tagctgccga
    26161 ccatgccaat cgcggctaac tacaatctct gttgattcca taactttgga acgccctgcc
    26221 cgacaaacta aagaactatg atagccatag tgctctctgt ctgtcggtca gaacgatata
    26281 attaatgatt aattaaaaat tgatgactac ttagattaat aaaatcatca gcaatttcta
    26341 ataatttagc agaagtggtt tgccggaaac cagcgacttc tactaaacag ccagtagtgc
    26401 tctgaatata attaactaaa ggagcataat caccatcacc actaaccagg acgataacat
    26461 ctaatttgtg agataattta atagcatcta tactaattcc cacatcccaa tctccttttt
    26521 taactccgcc aggaaaaact tgtaaatctt tcatcaccac ctcaaaacct tgttgactta
    26581 aagcttcaaa aaaggccatt tcgtcattgt tatcactacg aataacatag gcaaccgccc
    26641 taatcaattt acgttcacca actacttcag ttaaaacttt atgaaaatta accttttttc
    26701 cgtaaagatt acgggccgaa tggtaaagat tagctacatc tactaaaaca ccgactcttt
    26761 gctccggatg ttttaccata ttattcctct gccccagcct caatatccgg ctcttcaata
    26821 tcttcttcag ctgcatctaa attcccttta gatattaatt ctgcctccaa acgcttacgt
    26881 tcttcctcct cagcggccat tctcttagca atccttaagt taagccggct agctaattct
    26941 aaaaacgccg cttcatcttc tttctgtaga tactttaaaa gacgacgacg ttcaccaacc
    27001 ttacgcagta aaccccgccg tgatgaaaaa tcgtggcgat gtttctttag gtgttcggtc
    27061 aactccttaa tttcttcagt taaaatagca atctggacct gagttgaccc agaatcgcct
    27121 tcgtgttgac gaaatttttg aacaatttct tgttttgtct gtttatctag cataattata
    27181 tgttcccagc agagataagg attattccct attccatggg gagtaaaaat aaagagccca
    27241 ctttgagctt tttctgttat aacattattg aaaaataaaa tcaagatgtc gtataatata
    27301 gtaatgatcc aagatgatat caataaattt ttagaatatt tagaagtaga tcggggatta
    27361 agcaataata cccttattag ctatcgtttt tatttagctc gatttgctaa ctttgctaaa
    27421 aatcaaggga tacataatgt cagtcaaatc aaccaagaat taatccaccg ctatcgttta
    27481 tatcttaata gactaaacaa tcgccaagat gaaaatctca aaaagaatac ccaaaattat
    27541 catcttattg ccctgcggca attcttaaaa tatcttagta aaaacaacat tgaaagtcta
    27601 gattctaata aaattgagct agccaaacaa gaaccacgac aggtaagttt tttagagggc
    27661 tatgacttag ctgctttatt acaagcacca atgactgcca aacaaccaaa tactttaatc
    27721 aaattacgag atcgagccat tctagaatta cttttctcta ctggcctcag agtaagtgaa
    27781 ctagctagac taaaaattaa tgatattaac cttaaaaaat ctgaatttac cgtaagaggt
    27841 aagggtaata aactacgttt agttttttta tctgaagagg ctaagtcagc tcttaaaaaa
    27901 tatttagaag cacgacaaga cactgaacca tttttattta ttagccatgg taaaattaat
    27961 caagcatcta gttctcagta tggtttaacg ccacggtcca ttgaacggct agtagaaaaa
    28021 tatgctttag ccgccggcat tactaagcaa ataaccccac atactctccg ccatagcttt
    28081 gccaccgacc tcttaattaa tggagccgac atccgcagtg tccaacaatt attaggacat
    28141 gcttctatta ctactactca ggtctatacc catgtaactg atcagcaatt gaggcaagtt
    28201 taccaaaatt ttcacggaaa aaatcgtaaa aacatagcca atgataaaag tcaaaaatga
    28261 ttatctttac tatttaccta atttgtgtta tataaatttc acccttaata ataattcaaa
    28321 tatatgacat taattcctac agttatcgaa aaagatggcc aaatagagcg agcctacgat
    28381 atttattcac gtttattaaa agatagaata gtttttctgg gtgaacctat tgatgaccat
    28441 attgctaatc taattattgc tcaattacta ttcttagatg ctgaaaatag taataaagac
    28501 ataaagtttt acatcaatac tccaggaggt tcagttacgg ctggcctggc tatttatgac
    28561 actatgcaat ttattaaagc cgatgtcagc accatctgtg tgggcatggc agcttccatg
    28621 ggagcagtgc ttttagcggc tggcactccg ggaaaacgct tagctttacc taatagcgag
    28681 ataatgattc atcaagttat gggcggtttt gagggacaag caacagatat caaaatccgc
    28741 tccgagcata tactaaaaat taaagaaaaa ctaaacaaat tgctagctca acacacaggg
    28801 caagaaataa aaaccattga aaaagatacg gaacgtgatc attatctaac gccccttgaa
    28861 gctaaaaact acggccttat tgataaaatt atcactcgct aatagccgtt cacaatttta
    28921 ttgaataatt ttactatgtg gggtcttttc taaaagacac cagaaatcgc cacaggttct
    28981 aacggcgtta aagccggcag ccgaataata agttgtctgc tgatcaacaa aatcttgagt
    29041 aacaccaact cgaaaaagaa gtgccaatga gcaggcttgt gggtcatcgc ctaatctttt
    29101 tctcaaatac cactctacta taccattata gcctagacca aattgattat cacccaaaca
    29161 aagctgacca tcatcaatgg ttggtgctaa ttcttttaat agtttaatgt cccgatcacc
    29221 taaaataata aactggttat aaaaaaagtt taaattttgc caggaaagat aagcacccac
    29281 tataataact accccggcca ccacaaggcc aagagggcgt ataagttgat acaaccgata
    29341 aagtaaataa gccgaaaaaa ttgctactgc cggagcaaag acaatgaccc caaaaggatg
    29401 agttatccat tgataaaaac gccaagcaac agctagcggc gctataagcc aaacaaaaag
    29461 ccagccaaaa tcttctggga tatgacgcga aaaataatcc accaaaaacc aaataagacc
    29521 taagccggca gttattaaaa agagccaggt ataatttaac tctcctcgat caacgacaat
    29581 agtcttaagc caattagaga aggggatact actatcggta cgaataccat aagcacccct
    29641 taaatcagtt aaaaagctag gcccctttaa aatatagaaa tggagaagat taaaggcaaa
    29701 tagcccaaca agggtaatag gaatgaaaat taacaaatac ttgcgagccg gaatcttccg
    29761 accagcgcga aagagtagaa taaataccca taaagccgcg gcaaacagat aataaaacca
    29821 agcaaataag ccgcctacga ccactgatag ccaaaacaaa atgaaataaa gagaacgtcg
    29881 tttagggtta ccccgagcat aaaacactaa taaagaaaaa gagatcaagg cggtggggat
    29941 agtaaagacg gtcagttcaa agctgcggcc ataataaatc atgccgggca aaagagcgaa
    30001 aattaaagta gttaaaaagg taaaggaacg gcgatgagaa atcttaaata aggcaaaata
    30061 aaaaagtgtc aggcctacaa tcatgcaaaa tagtaacccc aaacgggtag ttgcttcact
    30121 tactccaaaa atggcataaa aaactgtggt cggcaaaaga aaaaactgag gatgatgagt
    30181 atagggtagc ccaacagccc cccaccggtc aaaaaaacca aattttaaat taaaccagcc
    30241 ataatgatta agattaatag ccgccacacc attagcgcct gctacatcat cagtcacatg
    30301 aataaaaggt gtattaattt tataaagaga tagcaaaata taaatcaaca aaacaatagc
    30361 taaccctcca atagtcagcc agtctggacc tttatcttct aaacggccac gtaattgaaa
    30421 taaaaacttc aaattcatcc agccatgttt ccagggaaca agcttagagt taccagtacg
    30481 ggcagcatag ggaattggtt tttcggcaaa agcaatatcc ttatttttag cggctaaaat
    30541 tttaatctct tcagaaaaag gcatattatt atgctgagga tttattttct ctaaaacaga
    30601 acgtcgaaaa acccacatcc ctgactgaga atctttcagc tttaaaccac agataactcg
    30661 cacggcgcga ttaaaacacc agttgccaaa acgtctaata aaaggcatga ctgttttatc
    30721 ttgaagagga aaacgagccg ctgaaataaa atctaatttt tcttgttcca aggcatttac
    30781 taaatccggt atggcttcag ccggatactg accatcacca tcaagaacta ctaagatatc
    30841 tccatgagct tctttgaatc cacgccttat ggcagcgcca taaccctgag tagtctcccg
    30901 ataaacttta gcgccaagct gacgagcaat tttagctgta ccatctgtag aattattatc
    30961 tataactatt gcctcaacat tagctggtaa tctctttaag acattgccga tagagtcagc
    31021 ctcgttatag caaggaataa tgacagaaat tttgatatga ttaaacttta gaatttaata
    31081 ttattaatat tgtatcattt ttattaccaa attgctaaat atatcactct tgctttttgt
    31141 gcctaaacat gataggctaa aatcagttta tttatatatt aaattaagtt ttggtttctg
    31201 tcagctctgg ccgcttaagt ctgaagtgaa ctacttcctt ctttttacgt ttataaaata
    31261 ttattttcta tcaaattaaa taccattaag gctctcccgc tggctctaac caaagccgtt
    31321 attatcctat gaactatatc attgggctag ttgctatcat cgtcggccta attttaggct
    31381 attggctaca acaccaacta accattaaaa gagcagctaa tgctgaacat aaagctgaaa
    31441 acattattaa tgaagctagg gctaaagaaa aggatatctt acttaaagca caagataaag
    31501 ccctaaaact cattgaagat gccaaacaag aaaatgaagc tcgccgtaaa gaaattactg
    31561 cctggcaaaa ccgcttggaa aaacgtgaaa cagctttttc tcaaaaaatt ctagaattgc
    31621 aagacaaaca acaagagctt tatgacaaaa ttgccgaagt agaaacaacc aaagaaaaaa
    31681 ttaatcaact taaagaagag gagttggtta aattagaaga aattgccaag ttaaatacag
    31741 aagaagctaa aaaactacta ttagaaagag tagaaaaaga agcttcggat agtctcatgt
    31801 ctaggcttaa taaattaaat gaggaggcta gtgaacaaat tgaagaaaag tccaaaaatt
    31861 taatggctac cgccatggaa cgtctagctt ctacttttac tgcggaaatg actacaacag
    31921 tggtggacct cccaaatgat gaaatgaaag gcagaatcat tggacgtgaa ggccgtaata
    31981 ttaaaaccat tgaacaactg actggagtag aaatcatcgt ggatgatacc cctaatgcta
    32041 ttaccatttc tgggttttct ggtattagac gacagatcgc caaacgagcc ctagattatt
    32101 taattaaaga tgggcgtatt catcccacca aaattgaaga tgctattgaa cgagctaaag
    32161 aagaactagc tctagatatt aaaaaggctg gcgaagatgc tctctatgaa ttaggcatta
    32221 ccggatttga ccctaaattg gtccagatta tcggacggct aaaatatcgc actagttatg
    32281 gccaaaatgc cctaaaacat agcgtcgaag tagctcattt ggctggacta ttagctgaag
    32341 aattgggggc cgatgttact atagctaaaa aggctggact attacatgat attggcaagg
    32401 cagttgacca tgaagtaaaa ggcacccatc cgcaaattgg tggtgatatt gcccgtaaat
    32461 tcaacttacc tcaagaaatt atcgacccca ttgaaaccca ccacgatgac catcctcaag
    32521 gattaatatc agttatagtt caagtagctg acgccatttc atcgtctaga ccaggggcgc
    32581 gcaatgatag cttagaccaa tatattcagc gcttagaaga gttagaaaaa attgctagca
    32641 acttcaatgg cgtagataga gcttatgcta ttcaggctgg ccgagaaatt cgggtctttg
    32701 ttaaaccaga agaaattagt gatttatcgg ctcatgagtt agcagtacaa atagctcaac
    32761 agattgaaca agaattaaag tatccgggtg aaataaaaat taacgttatt agagaattaa
    32821 gagtcattga atacgcccgc taaaaacatg tctatcaaaa aaatattcga caagacaaaa
    32881 aatcataaaa ccagcgacca atcgcttagt cagcatgtac ttgaccaaga aattatcttt
    32941 cgtctatcta aggggcgtct accaggtcgc cggcaatggc attatttacc aaagttttta
    33001 tctaaaaaag aaaaaattat tatcataatt tgtctcattg ccattttgtc tagtttaagc
    33061 ttctttttat ataattatta tcaaaaacac tgtgaaaaaa tccctcaaaa tgggggatat
    33121 tatgttgaag gtcttattgg tacacctcgc tatatcaacc cgctatattt aacctttaac
    33181 caaactgacc gcgatttagc ccggctaatt tattctagct tatttactcg tgacgctcag
    33241 ggccacctta ttaatgactt ggccgagagt tatgaagtaa gtcctgatca tcttacttat
    33301 ataattaaaa taaaagataa cgtcaaatgg agtaatggcc aaaacttaac ggccgaagat
    33361 gtccttttta cttatacggc cattatggac caggcctatg gctctccgct acgtaattct
    33421 tttagtggat tagaaataaa caaagttgac gacttaacta ttaagtttac tttaagccaa
    33481 ccatataatg ccttcctgga tctcttaact attggcatca tgcctaggga actatggggg
    33541 caaattccag ctagcagtgc tagtttggct gatctgaacc ttaagccaat cggcagtggt
    33601 ccttaccaat ttaaatctct aactaaagat aaggccggta atataaagtc ctatgaactc
    33661 actgtcaatc cttattattt taggcaactt ccctacatcc ctaaattaac ttttaaattt
    33721 tataataatt atgaagaatt agcagctgct ttaggtgacg gctctattga tggtgcagct
    33781 tatttgcccc aaaaaactgc ggaagactta ataactacta gacgctacga ccttaaatat
    33841 ctaggtactt ctcaactagt agccatcttc attaatagcg aggacaaagt tcttaaagat
    33901 aaaaatatcc ggcgggctct agccctatct attgacaaaa ataaaatttt actagacaat
    33961 aataacttaa ttgggcatct agccaccggg ccaatattaa accataaccc atactatgat
    34021 aaaaccatag ccgaggttag ctataacccc accgaagccc aaaaaattct cactgacagt
    34081 ggctggagtt taaaaactat caccactgac gatgtatctg ccgctcagca aaaattacaa
    34141 aataatcaag gtgaccaaaa tgaagctaaa accatcattg acttaggccc aggccaatgg
    34201 ttacaaaaaa ataatcaata ccttaccttt aatcttacag tagtcaacga gccagactat
    34261 ctagctatag cccaacagat tggccttgca tggcaaaaaa ttgggattaa aacttatcta
    34321 gatgtcgttg atccagaaaa tattaataat caaattattc gccgaaaaaa ttattctctc
    34381 ctgctttatg gccaaaattt aggctcagaa aatgatttag ctgctttttg gcactccact
    34441 caaagcggcc ccaatggtct taatttaagt aattatcgta atgagcaagt agacaaaagc
    34501 ctagaaactc taagaacatc aattgatgaa gaagctaaaa agaaagcagc ccagaccttg
    34561 caacaacaaa ttgctactga tttaccggct atcttcctct ataatcctac ctatctatat
    34621 atccaaaata aaaaaattaa aggttttgct atggaggtta taattgaacc agctgaccga
    34681 tttaatcaaa ttaatgaatg gtatattaag acaaaaataa agtggcaaaa aactcactaa
    34741 gcccaagtgg cggaattggt agacgcgcaa cgttcaggtc gttgtgttcg taagaacgtg
    34801 gaggttcaag tcctctcttg ggcactaaaa ttaactcttt tattattcaa ttaggccaaa
    34861 taaaggcaca atcattatga tgactaaata ccaaactaga aattatgcta gccgtcaaac
    34921 tggcaaatct tatagcccat ctagaaaacc taggcggact aactactatc aacaaacgct
    34981 taattctaca aagtcagaac gcgggaaact caaagttatt gttcttggtg gcttagaaga
    35041 agtcggccgt aatatgactc tactggaata tgaccaagaa attattatta ttgacatggg
    35101 tctgcaattt ccagaggagg atatgttggg aattgactac attattccta atactgacta
    35161 tttagtgggt aaagaagatt ggattaaggg tattattatt actcatggac actatgacca
    35221 tattggagct attcctcatt tactaggtaa actgaaaaat ccccccatgt ttatgggtcg
    35281 gctaaccgct gggctggtag aaaaaaagat taaagaacag ccccagtgcc ctaaagccgc
    35341 cattactata attgaagaaa ataaagctat ccaattaggc aaaaacttta atattgaatt
    35401 tctaagaatg aatcattcta tcccggattg ttttgccccc attattcata ctcccgtagg
    35461 aaccatcatg cataccggtg attttaaaat cgattattct ccagttaatg ataaaccagc
    35521 tgaccttcaa cgtattgccc aatttggcag ccaaggtatt ctgctattaa tgtctgattc
    35581 cacggattca actcatcctg gctaccagat ttctgaatcc agcattggcg atgaaatgag
    35641 tcggctcttt gaaaaaattg aaggacgtat cattatctgc actttcgctt cccaaattag
    35701 ccgtattcaa gaaatattta ctctggcgga aaaatttggc cgccgtattt atatccaggg
    35761 ccgcagtatg aatgataaca tcgagatagc taaacaaatt ggctatatga aattcaattc
    35821 tcagactatt attcatgaaa atgaaataca ccacctgcca gacaataaaa taatcgtctt
    35881 gggcactgga gctcaggggg aaaataatgc ctttctaatg agggtagtca ataatgaaca
    35941 tcgcacggtc cgacttaaac caggagatac catcattttt tcttcttcag ttattcctgg
    36001 caatgaacgc agtatccaaa accttaaaga tcaaattgtg cgccagggtg gcaaggtcat
    36061 tcactatgaa atgatggatg tccatgctgg aggccatgcc aagcaagaag acttaaaact
    36121 catgatgcgt ctcctaaaac caaaatattt tatgcccatt gagggtagtc attttatgct
    36181 ccaggcgcat gctgaactgg ctcagcaagt gggtattccc actaataaca tctttgtcgc
    36241 tgacaatggt caggtagtta cctttgaggt tcgtggtcac gaaaccattg gacaattaac
    36301 taaagaaaaa gtaatgactg attatgttat ggtagacggc ctaggggtag gtgatgtatc
    36361 cgatattgtc ttaagggacc gcaaaactat ggctgaagat ggcatgattg tagttattgc
    36421 caccattgac tccaaaactg gcgacccaat tggcaatcca gatattattt cccgtggctt
    36481 tatttatatg aaagataata aagatttaat tcaggatact agaatgcggg tcaaaaaaat
    36541 cattaaagag tctgatcctc tactattaac tggcacccga ggattgggag aagatgacca
    36601 gctaaaaaat aaaattagag ccgatgtcag ccaattctta tttaataaga ctaaacgacg
    36661 cccgatggtc ttgccagttg tcattaaagt ctaagatctt ctggactaaa aaacaaaaac
    36721 gcccaaaaag gacgctttcg tttgtgtagg ctttgacaat aaagctttaa taattaaagt
    36781 ataacaaaaa tagatagtaa ggtcaaacta tctattatct ccactgccat gaagctgacc
    36841 cctctcctga cgagataata atttatctag attattctga gcaatagcgt ctagatctaa
    36901 acctagttcg gttgctagct gtgccagata ccataaaaca tcacccaatt ctttagctat
    36961 ggcttcgcgt tgttcttcgc ctactacacc atccccatca cgaaaaactt ttttaatctt
    37021 ttcagccact tcgccacttt cacctactaa acctagagta ggatagacaa aacccttttc
    37081 caccaccgga taaactgcgg ttctccggct ttttgtttga tactctttaa attccataag
    37141 tttatgttat tatggccgtt gctccaacgg tcaagatagt tacaataata ccggcagcaa
    37201 taacaccgct aaaaattggc caaaaagctt tttttaaagg aaagccaaac aaccacgcgg
    37261 caatagagcc agtccaggca ccagtaccag gcaaaggaat ggccacaaac ataaacaaag
    37321 caataaggcc atacttttct aatttttgcc aagttttccg acgggtacgc ttaaaaattg
    37381 actctaaaat ctttttaaaa aaagaagagt gtttttctac aagctctacc aatttattaa
    37441 gtcctaaaat aataaaaaca ataggaatgc tgttgccaat aatagctaaa attaaagctg
    37501 taagtggagg cacatgccag acagtaatag ctaaaggaat agtcgctcgt aactcagtta
    37561 ttggtatagc tgaataaatc aaggtaagca gttcctttga cccaataagt tcttgtaaaa
    37621 actccatatc atttttaata tgacgccctg agtttagaag ctaactcttg aaggtgtttt
    37681 ggatcagcta ggggaccgct agccgaagac aaccaacctt cataagtgga aaaccattgt
    37741 gctggcccag aagaataaga ccgccactta ccttccttta aatgtccagt cccatgcatt
    37801 ggaataagct tgagatgagc gtgattaata cccatgccct ccataataac ccctactcgc
    37861 ccaacatcgg cataataatt tttaagaatc tgagctacct gtttagtggc taccatgaac
    37921 ctgcacaata cttcatcggg catggccagc acatcgctgt cataatgttt tttaggaata
    37981 acacaagaaa acccttcagt attagggtca atggctaaaa aagccataaa atcactatct
    38041 tcccagaaaa taccgggagt agttaaccga ccagtagcta tatcacaaaa taaacaggag
    38101 ctattatttt ctttttggga gtgattagtc atactattaa ttaatttaaa ttaagaataa
    38161 accacaaaac tatcaataga ttagcataaa attagaagat taccaatact aaaaaattaa
    38221 taaaaagccc gcatcaatta tacataaatg cgggcaaaat agttaactga catagtccat
    38281 aatgactttt atgtcctcta aaataagagg aacgttcccg gtcacaaaat aaaaacattg
    38341 cgccattatg caatgccagt ccgttacctc ctgatcatca taacttaagt ctggcctcac
    38401 gctagctttt agctccttct cgtgcgccag ccaataagat gcaggaaatg tccacaggcc
    38461 atagacatcc cccagccaaa cgttatcata attaacctca accccttttt cggtgcgatt
    38521 catcccataa ggatcccgcc cgcaccgctc gacacttttt tccagccgcc ggatagcgtc
    38581 agctacactt tgtgggactt tcttctggtt tttctcgata gtctctgcca ggacagagac
    38641 tctcccgctg tcctgaattg gtgagattat ttgaaaaaac tcaacaatgg cttctataga
    38701 ataagcctcc gcctccaccc ttttcagtct ctcaacgagg ttggtattta taacaacctc
    38761 ttgacagaat aacttaatct gtttttttaa agtcctgctt ctcatttttt gttcctcctt
    38821 ttaaaggtac gtctatcatt taatatggta taatatacca taaaaaacta aatctgtcaa
    38881 tagcagatat caaaagagat actgcatact tagatacact aaattcagtt taactaatat
    38941 tagccctaat tattgtttcc tttttggcag gcaccggtat aaa
//
LOCUS       DAOOIS010000005        25041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_2057_length_25041_cov_39.439526, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000005 DAOOIS010000000
VERSION     DAOOIS010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 25041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25041
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2057_length_25041_cov_39.439526"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /gene="groL"
                     /locus_tag="PK960_01215"
     CDS             complement(<1..1129)
                     /gene="groL"
                     /locus_tag="PK960_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965990.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPD07826.1"
                     /translation="MAKQIIYDDKARAALKKGVDQLANAVKVTLGPKGRNVVIDHGFG
                     SPTITKDGVTVAKEIDLDDKFENVGAALVKEVASKTNDTAGDGTTTATVLAQAMINEG
                     LKLVAAGINPIEIRQGIEKEVGEVIGRLKKMSKQISTKEEITQVASISANDPEIGKII
                     AEAMESVGNEGVITVEEGQSFGVEKEVVEGMQFDKGYVSHYMVTNTETMKAELDDPYI
                     LITDRKVAAVQEILPLLEKVLQIGKKDLVVIADEIEGEALTTFVVNKLRGVFNVVGIK
                     APGFGDRRKAMLEDIAILTGGRVISEEVGLKLETADLSDLGQARRVIVSKDNTTIVDG
                     KGDKKAIQERIEQIKKEQELADSDFDRDKLQERLAKLAGGVA"
     gene            complement(1156..1446)
                     /locus_tag="PK960_01220"
     CDS             complement(1156..1446)
                     /locus_tag="PK960_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPD07827.1"
                     /translation="MKLQPLHDQVIIKPLTAEEKTKSGIILPDTINKDRPEQGEVIAV
                     GPGRLLDNGQRAPMSVKVGDKVVFKKYSTEDLKLDDQEYVVVAERDILAIIN"
     gene            complement(1533..2549)
                     /gene="gap"
                     /locus_tag="PK960_01225"
     CDS             complement(1533..2549)
                     /gene="gap"
                     /locus_tag="PK960_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPD07828.1"
                     /translation="MDRPLRVAINGFGRIGRSVFKAMVNKPQLEVAAVNDLTDNLTLA
                     HLLQYDSCYGRYDHTVSYDDDSIIVDGHHYLALAEKDPSKLPWSKLGIDVVIESTGRF
                     TKGEEALAHVQAGAKKVIISAPAKDESTKTIVLSVNEAELQVSDQVISMASCTTNCLA
                     PLTKVLVDKIGVSKAVMSTIHAYTADQNLVDGPHKDLRRARSAALSIIPTTTGAAQAV
                     AKVIPELNGQFDGSAFRVPTPVVSLCDVVFVTKRAVSVEEVNNILREAASHDRFGALT
                     VTDEPLVSVDFTGNPASSIVDLSLTTVIGGDLVKIVAWYDNEWGYSQRLADLAAYIAD
                     NNLV"
     gene            complement(2601..3176)
                     /locus_tag="PK960_01230"
     CDS             complement(2601..3176)
                     /locus_tag="PK960_01230"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPD07829.1"
                     /translation="MIISFAGWPGSGKSTIAKRIATELGWPYYSMGQLRREAAKRRGM
                     TLEEYNKLAMSDISTDLEVDEYQTKLGQEQDNFVIDGRTSWHFIPQSYKIFLNVDPRV
                     GAERIFQELQQGGDRNEGHNLTTVEAVLNSNYERVQNDRKRYQKYFGIDVYDLANYDE
                     IVDTTNLTIEEVVAKVRSLIKQRLAQDKVKN"
     gene            complement(3181..4113)
                     /locus_tag="PK960_01235"
     CDS             complement(3181..4113)
                     /locus_tag="PK960_01235"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPD07830.1"
                     /translation="MEQLIVGEGGGRLDKFLAAKVPKYSRSAWRQFINDSLVKVNGRT
                     VKPHYFINKGDIISWSISSSILKSQPNKKISRVSLPLKIIAEKPDYLVINKPAGLLTH
                     DNFWPPRQPTVVGKLAKKYPEILSLGDNPTRPGLVHRLDRDVSGLMVIPRTEPMFNAL
                     KRQFKNHTVRKVYTGLVYGTLPQTQGVINFKLGRSTNGRKMAARPFNQAGKSALTEYE
                     VIRQWTDYTLLKIIIKTGRTHQIRAHLAAIGHPLVGDNWYGTNKTKIKNKALGINRVF
                     LVATQLSFLDLAKVRQTFSLRLPRQMTSFFNTLK"
     gene            complement(4116..5231)
                     /locus_tag="PK960_01240"
     CDS             complement(4116..5231)
                     /locus_tag="PK960_01240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD07831.1"
                     /translation="MKQPLIANFSQEDQSLVYELLTTWADGRAVLWSITGPLTNYWSA
                     QAWPLRLINKKTTLSPFWIGLTLPWRKLIKRRQLTKLIRQYSLDGLICLSWLDRLLLT
                     GPACRLGLRVVWLELPEEAKLSLPNWLLSYYRHQAAKVKIITFGRHLVDHLRLQGQSL
                     NNCYLLLPAIDPKRHLSQTDLFSNLAINKQEQSNKPYFLIGSVVDLCITHPTEILLRS
                     LKIVLSVIPSVQLVIIGDGPKRKELQELARELDLANLVWFVGHQDPLDKWLYSLDGII
                     ELEVSKSQAWQRLLQAMLAGLPIIAPKSKLTADFLLPGETALVVEQAEPEMLAQAVIQ
                     LQQNKLLAGRLACHSKTWINEFGLIDRQLNDFVHYIS"
     gene            complement(5209..6675)
                     /locus_tag="PK960_01245"
     CDS             complement(5209..6675)
                     /locus_tag="PK960_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HPD07832.1"
                     /translation="MSLARKFAYNNIVQIIGKGISIILGVLGVALIARYLGVEGFGRY
                     TAVNNFLGMFAVLADLGLTMVTAQMINERPEDKQSILNNLFGFRLFTAGVIILIGLFI
                     AIFLPYYRELWFILAILAGSYFFVAINQILVGLLQSELKVDYLMVAEIAGRLLWLGGL
                     VLSNYLNWGLTGIALATTFSSMVNFSVAWFLSAKRVKIWPSYRADLWHKIAQRSWPLA
                     ITITLNLLYLRTDILFLSWWQTEEAVGLYGAAYKVIDILTSLPFLFIGLLLPLLTRAW
                     SNRDSQKFNFLVNNALLILLILVVPLIIGGQILATPIIKFIAGASFSAAGSILALLLV
                     ALAGIFVGCLFNHLLIAVNQQQVMVRPYLVVAITAIPAYYFLIRYFSYWGAAIVTVYS
                     ELLIAILAGYKAKKMAHWHWPTITSLKLLAANLLMVIVIWPCRHWAGQSIINLLAIIL
                     LGVVVYSLSLIGLKVINWSKLQVIFKPDNKNYETAPHC"
     gene            6740..7153
                     /locus_tag="PK960_01250"
     CDS             6740..7153
                     /locus_tag="PK960_01250"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07833.1"
                     /translation="MSKTHFLASGFISLAILLFTAWPTLTAAQTFSPGSQAGLTTIGN
                     KAYGTPGSPQDIRILIAKIVQITLGLLGTILIVLIIVAGVQYMTAGGNQTKVESALKH
                     LTNALIGLIIVLVSYAITVFIIQQLNLAIIGRQWT"
     gene            complement(7252..7602)
                     /locus_tag="PK960_01255"
     CDS             complement(7252..7602)
                     /locus_tag="PK960_01255"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07834.1"
                     /translation="MLNKFFIYFCLLATTLITPSAKAADPVSLSNPINGLDNILEGSA
                     YLGQVIGRVLGIIGAVALLLLVYGGISMIISGGNESKITKSKSIIVYTLIGLVIIFSS
                     YLIIDFTIKSIRGT"
     gene            complement(7608..8309)
                     /locus_tag="PK960_01260"
     CDS             complement(7608..8309)
                     /locus_tag="PK960_01260"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HPD07835.1"
                     /translation="MTKSRYFFGIILFGCALLVAAPSALAVTTCAETVPGTNTAKCLK
                     TCTDDIIYMVPYPGGDLGCKNSSESAGDKCCGTCTIHDLKTKGAARCLDICTDPYPSH
                     DGKGDKHCPPGKKCCYMPLVAIPPTTLDQSQTNQTESKPLDESALPKFNSLLTANVNT
                     VIGNIIKSLLGLTGTLTLVMMVVAGIMWMTAGGKEQTITKAKKMITWTAIGLAIIFSS
                     YIILKSVFDVLTTNP"
     gene            complement(8367..8438)
                     /locus_tag="PK960_01265"
     tRNA            complement(8367..8438)
                     /locus_tag="PK960_01265"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8403..8405),aa:Glu,seq:ttc)
     gene            8523..9569
                     /gene="tsaD"
                     /locus_tag="PK960_01270"
     CDS             8523..9569
                     /gene="tsaD"
                     /locus_tag="PK960_01270"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPD07836.1"
                     /translation="MKILAIETSCDETAAAVAHSTANHIIIDSNIVASQIDIHQVFGG
                     VVPEIAAREHVKQILPVIEQALHSAGLKRLEASHNLDGLAVTVGPGLMTSLLVGVETA
                     KTLALAWNLPLIPVNHIAGHTNANFIDRDLSSIKFPLLTLTASGGHTNLLLMHNPLHY
                     KIIGQTMDDAAGEAFDKAAKLLGLGYPGGPKISMMAKKFAEQHQETTIQLPRPMLKRT
                     DYNFSFSGLKTALLYKVREDLTWQDHVAEYAYVFEKTVVETLVRKTIKAASDLKVKTV
                     ALAGGVSANECLRSNLKATLEQKLPQVDLLLPNMAYTTDNAAMIATAGLLKMQAGLTQ
                     DLEQIIAQPNYSLK"
     gene            9580..9888
                     /locus_tag="PK960_01275"
     CDS             9580..9888
                     /locus_tag="PK960_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07837.1"
                     /translation="MTSEDIKKLVESLKNEDLYTFEQRCKEFFYNHSYEFASIDQNNR
                     DFIINFLKNHRNDVVSYGGIPGYKLDNELYKIHGQLSEYGLSELDYNNFKKITNYFRC
                     "
     gene            complement(9885..11252)
                     /locus_tag="PK960_01280"
     CDS             complement(9885..11252)
                     /locus_tag="PK960_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPD07838.1"
                     /translation="MQTQPSVFKAYDIRGIYGQDFDETLAYDLGLAYSQWRRQELSTD
                     SLSVVIGHDMRLSSPGLHQALSRGLVDGGVEVIDIGLCSTPSFYFAVANESADGGLMI
                     TASHNPKDYNGFKIVRDRARPVSESTGLTELKNLVFNNKLAPAKRLGTIRKIDNILDQ
                     HVAYELAIAGFQPLKPFKIVADPANGMGGPYLEKLLASYSTISLIRMNWALDGSFPSH
                     ESDPLKPENIEPLCQMVLGQQADLGIATDGDADRVFFVDNLGRPVEPGILRALMAKIF
                     LEERPGATIAYDIRPGKITPETIIANGGQPLVTKVGHSFIKEATLESGAYFAAESSGH
                     FFLNMEHGCYEVPGVIIIKLLQALSKTHETLAELIKPYQKYYNSGEINRRVSDPAAKI
                     KLIAERYSDGQLDYLDGLTVTYPDFWFNIRPSNTEPVLRFSLEAKTPEIMTTKRDEIL
                     KLLAN"
     gene            complement(11254..12396)
                     /locus_tag="PK960_01285"
     CDS             complement(11254..12396)
                     /locus_tag="PK960_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07839.1"
                     /translation="MVKTRQLFIWLLSLGVLIIPFTTKAFVGRTGNEVTFGPADQLNG
                     VTYALGQQITIDTPLPDDLICLGGDVFINQPVAGDVICAAENLNIKAPVGGSVRLFGS
                     NLNIDSQIGRNAILSGSQLLLGDNSEIKGETLLANLLIESRGIYRKAVHGLYGQAKLA
                     GHYEAPIDLKEIQRGASQLIVDPTANIGAGLSYRAEKSAQIDSGAHINGEVVHKVATA
                     QEKRAAMVGWWWSRFLHLFMLLVTALVLYSLWPKLGERLVETGKHKAGLVCFYGFLTL
                     FILPIIAIAAGFTIIGIPLALIALAIWCFLIYIGCLVPSYWLGLKIIPKAKNHLWPFL
                     LGATIIVAIGWIPFIGQIITMLATWWGVGAIEQTIFKAHSTKKANH"
     gene            12456..14057
                     /locus_tag="PK960_01290"
     CDS             12456..14057
                     /locus_tag="PK960_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HPD07840.1"
                     /translation="MNIKEKHQKEEKIIQSLVAKQPQNVQALAHWKRQLSRITGQPII
                     SNNQLLAIYFKLTPPDKIDPNLVQLLKKRAVRTMSGVAPVAILTKPYPCPGHCRYCPQ
                     QVNVPTSYLANEPAVMRARHCHYDPIKQIHYRLRALTANGHQPTKIELIIIGGTWSYL
                     PKKYQYWYIANCYAAANTYGKTARRYRQNLSLDQLKKAIAKEQTINESTRYRIVGLTL
                     ETRPDCLTPQELTRLREFGCTRVEIGVQIVDDHILRLNERTTTVQDIAQATQLLRDWG
                     FKITYHIMPALPGSNPNHDIAMYKKLFTDPRFQPDQIKFYPTVVVKGSKLYEDWLNGL
                     YKPYSDSELIRVIKECKAATPPYVRIVRLIRDIPKESIEAGNKITNLRQIIQKQGVQC
                     QCIRCREVKDKAVDWSNLQLIIRQYIAGDGEEYFLSWESPDQSTIFGFCRLRLPTKPA
                     SNPYLSDCALIRELHVYGLLQPLGSQGQIQHRGLGEKLLIHAEKIAKEHHYSKIAIIS
                     GVGVRNYYRKFGYYLNHTYLVKAVI"
     gene            14087..15040
                     /locus_tag="PK960_01295"
     CDS             14087..15040
                     /locus_tag="PK960_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07841.1"
                     /translation="MFNPEQFLRNLSERQEKRKTLNNKEYLQELSIKAQQMNNINRID
                     PYQFINLYGREAVMADLKWVKSRQAYFRTHDSETETWHKEVANILEAILHEQIEQNDY
                     FGENVWTIKTCDYDDYRNHIDSLLEIRHPERRFANYSGLALDVTSASRLEALAKKIER
                     IFNHIKRGQLAKIKYFQSDFLNIRGEKSNIPLFVVGCDVHHTEELAKLWGDGRARILA
                     EHPIQIALLYQITRQAQIYWDYAKRNNQKEVADQYEEILKEFEAITQERQTIITDIKN
                     RPESKEFLQTDIMLNNLEKILSEYDKYYQRPRSTTFYSRGK"
     gene            14988..16223
                     /gene="glyA"
                     /locus_tag="PK960_01300"
     CDS             14988..16223
                     /gene="glyA"
                     /locus_tag="PK960_01300"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPD07842.1"
                     /translation="MNIIKDQEVLRFIAEESKRQIDELELIASENYVSPAVLAALATP
                     LVNKYSEGYPGHRYYGGNHLIDEIENLAIERAKQLFQAEHVNVQPLSGSPANLAVYLA
                     LLQPGDTVLSLRLDHGGHLSHGHPVNISGRLFNFVHYEVDPQTGCLNMDQIRKIAKEV
                     RPRLIIAGFSAYSREIDWAGFQDIASEVGAFTLADISHTAGLIAGGQLINPTPLFDVV
                     TTTTHKTLRGPRGAIIMCQEKLAKKIDQAVFPGLQGGPHDNVTAAKAIALGEALEPSF
                     KKYAHQVIVNAQFLAKKLQEKGYTIVSGGTDNHLMVIDLRPQQITGREAEIALEKAGL
                     SVSRSTIPGDSRPPFNPSGLRLGMAAVTTRGFKEQEMEQIANWFDQALNKRDSKPALA
                     EIKQQVSTLGRRLPPPQYN"
     gene            16228..17094
                     /locus_tag="PK960_01305"
     CDS             16228..17094
                     /locus_tag="PK960_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HPD07843.1"
                     /translation="MPQQLINGHELAEEIKDSIANFVYSLQGPRPNLAIVLVGNRPDS
                     ELYVRLKQQEAKKVGIDTHLYRCPEDIKEQELIAIINFLNNDPLIDGLLVQLPLPKHL
                     NPDNIVNIIRADKDVDGFTPLNLQKLLNNDPSAIIPPVFGVILRIIIERQLDLTNKKA
                     VILGNSPIFSDNLALLLKRQGATVSIFHEVNKTASQAIEEANLLISALGQPLIVKPKE
                     LPENYLIIDIGITKKDHKVYGDVDLKSINPTINGWATPVPGGVGPLTIAMALYNTLLL
                     YQQHHAQSRDNK"
     gene            17069..19192
                     /locus_tag="PK960_01310"
     CDS             17069..19192
                     /locus_tag="PK960_01310"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPD07844.1"
                     /translation="MLSHETINNVLQTYFHFTQLRPGQESAIISAIKGQDTMVIMPTG
                     GGKSLCFQLPALILPGITMVISPLIALMKDQVDHLNALGIPATFINSSLTADEAKERL
                     GEALNKKYRLIYIAPERFYNQSFFKTVQQLPISLVAVDEAHCISQWGHDFRPSYLQLK
                     KFITALGRPPIMALTATATPEVREDIIKQLDLHQPQEIITGFARPNLSFEVISTADSQ
                     KKEIVLETIQKQPEASGIVYVSTRQRADELVRYLQDYHIAAVEYHAGLNTAERQKLQN
                     TFMSGRAKVIVATNAFGLGINKPDLRFVIHYDMPGTIEAYYQEAGRAGRDGQPSLCLL
                     LYSPRDRYLQEFFIAGDNPSLDLILDIYEFLTTYPNDHILITYSTIKNALGLDTPDLA
                     IGTALKILEREGYLAKTYEKTSQAMIQLAPKNIKLIDGQKALGPKAKKSLEIWGKLYD
                     RYNGKLRTGLYINLDTLANSLGYKKDSLARVLKKLNDQGIIIYEPPFKGTEITLLKRC
                     PREAVNLDRKALETKLAFAQHKLDQMENYVYHRGCRQQYILDYFGEHLAGNCGQCDWC
                     KGNFNIHSFNSRPNKLSQRTSYHKNLYNIQLSTKLTQLTTLELYQNGLNIQEIARERE
                     LQSETIVEHFCYLLEQGLITDMSQLIDENIITMVQDLLAQKPQLTTDSLKTLKEQLPS
                     YVDWADLKLTLAYLQHNKSHEPKTS"
     gene            19173..19901
                     /locus_tag="PK960_01315"
     CDS             19173..19901
                     /locus_tag="PK960_01315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD07845.1"
                     /translation="MNQKRADYFRQIVKNNYQEIAPEFAASRNRPLWPKLQEIINDIS
                     PQGSLLDVGCGNGRLLDLLVQQQINSYTGLDISSHLLELAAQKYGTSYGNIMIEWVEG
                     DLLEESLQCQFDNICCIATLHHIPSTQYRQLAANHLANWLKPGGYLIISVWNLWRQPQ
                     YWPKLIASYGQALLRQCEPGDIFLNWGQQVGDSYRRYYHALNKKELIKTITKSGLKII
                     HYDNDGLNHYLVAQKLPLSGNLSI"
     gene            complement(19872..21749)
                     /locus_tag="PK960_01320"
     CDS             complement(19872..21749)
                     /locus_tag="PK960_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HPD07846.1"
                     /translation="MSKRRFIWTIAIIILVIFIIDVARLGYTAWGAYQAGIEGKTQLT
                     TGGQALKRGDWSQAQEAMGQAQESFARARSLSLILVQHPLWSHIPWYREQATSAHNLS
                     KAADIACSTILELLGQAQQWLADLSQLSFNQLTTSSLPIALSQIQQSPALILNTLVSL
                     HEIENSLSAVNFGPGLSELNAPAKQALKALEDGKELLAEYLPWLQLAPYLAKQLSDGR
                     FLLVLQNNDELRPTGGFIGNYGLLELENGRITNLEVHDSYHLDMPAQDYFKPAPPKEL
                     NKYLGVKQWFLRDANWSPDFPTAARQIGYFYGEQIKVINKVSSTPKIDGVIAITPAVV
                     EDLLGIVGPITVEGQIYNQQNFVDLLQYRVEMSYDKYGVNSWNRKEVINDVIKELKEQ
                     LMALPSSRLPEIAKIITKNLKEKNILLFSYDPVIEQWLIKANWAGQARSYSGDYLWII
                     DANLAAYKTDAVVDKQINYKLYNNDDDWFAELILTYKHNGGFDWRTTRYQSYTRIYVP
                     RGSQLVSQSGFKDPATASEDLDKTVFGGFLVVEPQETKVITLVYKLPPDIAQQISSGH
                     YNLLLQKQPGRQRTSLTVDLNFPQSVKRAYSEEMPLKIKDKQVSGTSSWSLDTQITAE
                     W"
     gene            complement(21834..22214)
                     /gene="rpsP"
                     /locus_tag="PK960_01325"
     CDS             complement(21834..22214)
                     /gene="rpsP"
                     /locus_tag="PK960_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPD07847.1"
                     /translation="MLKIKLARIGKKKSPVYRLIISEAGRDPYGRILENLGSYNPKTK
                     DLQVKAERINYWLNEGAQMTITVNNLLVGHKIVTGNIIKIRRQQPVDQNKNKNKENDH
                     TDHSPAPGLEPAVPSESDADKKEE"
     gene            complement(22623..23717)
                     /locus_tag="PK960_01330"
     CDS             complement(22623..23717)
                     /locus_tag="PK960_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPD07848.1"
                     /translation="MFWQEEGLFIYQLDEEWWRAAWVADMPGAWMGNSWYYSSTKPIV
                     QMVVPYSSIVNPTGYIYWNGEISWGANAGFFDNILSEASVAVIKRHNRLRGDVNNDGV
                     VNDTDTALSLAAASGFIGLSFQDYLYTAGLNYAAGCVAVPWVAGGQINAYLQNLWVVN
                     PDNPLVQNLHIGELFTDDWGYNQLSPEISGQTITIPQNGDNLFAVEGIYPDGAPWSAA
                     ILLDGNNLLRFQQDAEEPIVELLNRQPVTFVMPDGVQFRQAISRLTGDYSTAVDDPVI
                     PVTLMSVGCYPNPFAASTTIKFVGNIPTKIEVFNIKGQKIQSLTNNSKTQSIVWDGRN
                     SQGKQVADGVYFIRATAENQMMTTKVIKIH"
     gene            complement(24273..24344)
                     /locus_tag="PK960_01335"
     tRNA            complement(24273..24344)
                     /locus_tag="PK960_01335"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24309..24311),aa:Gly,seq:tcc)
     gene            complement(24422..24496)
                     /locus_tag="PK960_01340"
     tRNA            complement(24422..24496)
                     /locus_tag="PK960_01340"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24459..24461),aa:Pro,seq:tgg)
     gene            complement(24519..24968)
                     /locus_tag="PK960_01345"
     CDS             complement(24519..24968)
                     /locus_tag="PK960_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07849.1"
                     /translation="MSSKFENYTSPSETKEQVSSQKKYQLLYDAPAGAYYVKEWTQEE
                     LADWENSGYRTQDDHYREVGLYDSQEELEKAIQELRGPKVFAVFEDQAAGEVYTTPED
                     NLTGNWRGSREGHANLVAVFENQEEAEKFVEERRAYYERLAAEHSDK"
BASE COUNT         8052 a         5499 c         4373 g         7117 t
ORIGIN      
        1 ctgccactcc acctgctaat ttagctaaac gctcttgcag tttatcccgg tcaaaatctg
       61 aatcagctaa ttcctgctct tttttaattt gctcaatccg ttcctggata gccttcttgt
      121 cacccttgcc atctacaatg gtagtattgt ctttggagac aatcactcga cgtgcttgtc
      181 ctaaatcact taaatctgcc gtctctagtt taagtccaac ttcttcagaa atcacccgac
      241 caccagttaa aatggcaata tcttcgagca tcgctttacg gcgatcacca aaacctggag
      301 ctttaatccc cacaacatta aagacacccc gcagtttatt aaccacaaaa gtggttaaag
      361 cttcaccttc aatttcgtca gcaatgacta ctaaatcctt ctttccgatc tgaagcactt
      421 tctctaaaag cggtaaaatt tcctgcaccg ctgccacctt acgatcagta attaaaatat
      481 aagggtcatc taattcggcc ttcatagtct cagtattagt caccatataa tgagacacat
      541 agcctttgtc aaactgcatg ccttcaacta cttctttttc tacaccaaat gattggcctt
      601 cttctacagt aatcacccct tcattgccga ctgactccat ggcttcagca ataattttgc
      661 caatctctgg atcattagca ctaatggaag cgacttgagt aatttcttct ttagtcgaaa
      721 tctgtttact cattttcttc agtctcccta taacctcacc cacttctttt tcaatgccct
      781 gccgaatctc aatggggtta ataccggcag ccaccaattt aaggccctcg ttaatcatgg
      841 cttgagctag gacagtagcg gtagttgtcc catcaccagc cgtgtcatta gtcttagagg
      901 ccacttcttt tactagggca gcgccgacat tttcaaattt atcatccaaa tctatttctt
      961 tagctaccgt caccccgtct ttagtaatgg tcggactgcc aaatccatga tcaattacta
     1021 cattacggcc cttgggacct aaagtaacct ttaccgcgtt ggctagctgg tcaactcctt
     1081 tcttaagagc agcccgagct ttatcgtcat aaataatctg ttttgccata tagttctaaa
     1141 attaaaaatt ataaattaat taataattgc taaaatatct cgttcagcaa ctactacata
     1201 ttcctgatca tctagcttta aatcctcggt agaatatttt ttaaatacta ctttgtcccc
     1261 aaccttaaca ctcataggag cacgctgacc attatctaaa aggcgaccag gaccaacggc
     1321 aataacctcg ccttgttcag gccggtcttt attgatagta tctggcaaaa taataccaga
     1381 cttagttttt tcttccgccg ttagcggctt aatgataact tgatcatgta atggttgtag
     1441 cttcataata gtaaaattaa attattaatt agcagtctaa cttacagact gctaatagta
     1501 tagtcaccct ctctaacctt gtcaaggcct aattaaacta aattattatc ggcaatatag
     1561 gcagccaggt cagctaaccg ctgactatag ccccattcat tatcatacca ggcgactatt
     1621 ttaaccaaat caccgccaat cacggtggtt aaagataaat ccacaataga acttgctgga
     1681 ttaccagtaa aatctaccga caccaatggt tcatcggtta ccgtcaaagc accaaaccga
     1741 tcatgactcg ccgcttctct taaaatatta ttaacttcct caacgctaac tgcacgttta
     1801 gtgacaaata ccacatcgca gagcgaaact actggcgtag gtacccggaa ggccgagccg
     1861 tcaaactgac cattgagctc aggaatcact ttggctactg cctgagccgc accagtagtg
     1921 gtaggaataa tacttaaggc ggcgcttctc gcccgacgta aatctttatg cggaccatca
     1981 actaaatttt gatcagcagt ataggcatga atggtactca taacagcctt gcttactcct
     2041 attttatcta ccaatacttt agtaagtggc gctaaacaat tagtcgtgca ggaagccata
     2101 gaaattactt ggtcactcac ctgtaattca gcctcattca cacttaaaac aatagtttta
     2161 gtagactcat ctttagctgg cgcactaata ataacctttt tagccccagc ctgcacatgg
     2221 gccaatgctt cttcaccctt agtaaaacga ccagtagatt caatgaccac atctattcct
     2281 aatttactcc agggcaactt gctcgggtcc ttttccgcta aagctaaata atgatgacca
     2341 tcaacaataa tggagtcatc atcatagcta acagtatggt cataacggcc ataacagcta
     2401 tcatactgta aaagatgagc taaagttaaa ttatcagtta aatcattaac agccgcaacc
     2461 tccaattgag gcttattaac catagcctta aagacgcttc ggccaatgcg gccaaaacca
     2521 ttaatagcta ctcttaaggg acgatccata gatttaattt aattatatta ataaaaaaca
     2581 ataatagttt agaaaaaggg tcaattttta accttatctt gggctaaacg ttgctttata
     2641 agagacctaa ccttggccac tacttcttca atggttaaat tagtagtatc tacaatctca
     2701 tcataattag ctaaatcata aacgtcaata ccaaaatatt tctggtaacg ctttcggtca
     2761 ttctgaaccc gctcataatt actattaagg accgcctcca ccgtggttaa attatgacct
     2821 tcgttacggt caccaccttg ctgtaattcc tgaaaaattc gttcagcgcc tacccgtggg
     2881 tctacattaa gaaaaatttt ataagactgg gggataaaat gccaggaagt ccggccgtca
     2941 atgacaaaat tatcttgctc ttgccctagt ttagtttggt attcgtcaac ctctaaatcg
     3001 gtagaaatat cggacatagc taatttatta tattcctcta gggtcatacc ccgccgttta
     3061 gccgcctcgc gacgcaattg acccatacta taatagggcc accctaactc cgtagcaatc
     3121 cgtttagcaa tggtagactt gcctgaacca ggccaaccag caaaagaaat aatcataagt
     3181 ttattttaag gtattaaaaa atgaagtcat ctgccgcggt agacgaagac taaaggtctg
     3241 ccgtactttg gctaaatcta aaaaagataa ctgagttgcc actaaaaata cccgatttat
     3301 acctagcgcc ttatttttaa ttttagtttt attagtacca taccaattat ctcccacgag
     3361 tggatgcccg atagctgcta aatgagctct gatttggtgt gttcgcccag ttttaatgat
     3421 aatttttagt aaagtatagt cagtccactg acggataact tcatattcgg tcagggccga
     3481 tttacctgcc tgattaaatg gccgagccgc cattttacga ccattagttg aacggccaag
     3541 tttaaaatta atcactccct gagtttgcgg caaagtacca taaactaaac cagtataaac
     3601 tttcctaaca gtatggtttt taaactgacg cttaagggca ttaaacattg gctcagtgcg
     3661 gggaattacc attaaaccgc tgacatctct atctagccga tgcactaagc ctggacgagt
     3721 gggattgtct cccaaactta aaatctccgg atatttttta gccaatttac cgaccacagt
     3781 gggttgccgc ggtggccaga aattatcatg agtcagaaga ccagctggtt tattaataac
     3841 tagataatct ggtttctctg ctataatttt gaggggtaaa ctaactctag aaattttctt
     3901 attaggctga gatttaagaa ttgaagagga aatagaccag gaaataatat cgcctttgtt
     3961 tataaaataa tgaggtttga ctgtccggcc atttacttta actaaggaat cattaataaa
     4021 ctgacgccag gctgaacggg aatacttggg aaccttagcg gctaaaaact tatctaaccg
     4081 accacccccc tctccaacta ttagctgttc catatttaag aaatataatg gacaaaatca
     4141 ttaagctggc ggtcaataag accaaattca tttatccacg tcttactatg gcaagccaaa
     4201 cggccggcta ggagtttatt ctgctgcaac tgaataactg cttgagccag catctcgggc
     4261 tcagcctgct ctacaactaa agctgtttct cccggtaaaa gaaaatcggc cgttaattta
     4321 cttttaggag caataatggg cagtccagcg agcatcgcct gcagtaagcg ttgccaagct
     4381 tgagatttac ttacctctaa ttctataatc ccatctaggc tatacagcca cttatctagc
     4441 ggatcctgat gaccgacaaa ccagaccaaa ttggccaaat ccaattcacg cgccagctct
     4501 tgcagttctt tacgtttagg accatcacca ataataacta attggacact aggaataacc
     4561 gataaaacaa ttttaagaga acgcaataaa atttcggtag gatgagtaat gcataaatct
     4621 actactgagc caataagaaa atagggctta tttgactgtt cctgcttatt aatggctaaa
     4681 ttagaaaaca aatcagtttg actaagatgc cgtttggggt caatcgctgg caaaaggaga
     4741 taacaattat taagtgactg tccttgaaga cggagatgat caactaaatg acgaccaaaa
     4801 gtaataattt tgaccttagc cgcctgatgg cgataatatg ataaaagcca atttggtaaa
     4861 gatagcttag cctcttccgg caactctaac cacaccaccc ttaagcctaa acgacacgct
     4921 gggccagtaa gcaatagcct atctaaccac gaaagacaaa taagtccatc cagtgaatat
     4981 tgacgaatta atttagtcaa ttgccgtctt ttgattaact tacgccacgg taaagttaaa
     5041 cctatccaga aaggacttaa ggttgttttt ttattaatca aacgaagtgg ccaagcctga
     5101 gccgaccaat aattagttaa gggaccagta atagaccaaa gtactgcccg gccatcagcc
     5161 caggtggtta aaagttcata aactaatgat tgatcttcct gactaaaatt agcaatgagg
     5221 ggctgtttca taatttttgt tatccggctt aaaaattacc tgcaatttag accaattaat
     5281 aactttaagt ccaattaaac taaggctata tactacaacg cctaaaagga taatagctaa
     5341 taagttaata atactctgtc ccgcccaatg acggcaaggc caaatcacta taaccataag
     5401 gagattggcg gctaataatt ttaaactagt tatagtaggc caatgccaat gggccatctt
     5461 tttagcctta taaccagcta ggatagcaat gagaagctcg ctatatacag ttacaatcgc
     5521 cgctccccag taagaaaaat aacgaatcaa aaaataataa gctggtatgg ctgtaatggc
     5581 taccaccaaa taaggacgca ccataacttg ttgctggtta acagcaatga ggaggtgatt
     5641 aaaaagacag ccaacaaaaa taccagctaa ggccacgaga agcagagcta aaatagaacc
     5701 agcggcgcta aatgatgccc cagcaataaa tttaataatg ggggtagcta aaatctgtcc
     5761 gccaattatt agggggacaa ccaaaattaa taaaataagc aaagcattgt taactaaaaa
     5821 attaaatttt tgactgtctc tgttagacca agctctagta agtaggggca aaagcaggcc
     5881 aataaacaaa aagggtaaac tggtaagaat atcaatgact ttataagcgg ccccataaag
     5941 gccgactgcc tcttcggtct gccaccaact tagaaaaagg atgtcagttc gtaaatatag
     6001 taaattaaga gtaatagtta tagctaacgg ccaagaacgt tgcgcaattt tatgccataa
     6061 atccgcccga tagctaggcc agattttaac tcttttagca cttaaaaacc aagctacact
     6121 aaaattaacc atactagaaa aagtagtagc cagagcaata ccagtaagac cccaattcaa
     6181 ataattactt aaaactaaac cgccaagcca taataaccgg ccagcaattt cggcaaccat
     6241 taagtaatca actttaagct cactttgtag taaacctacc agaatttgat taatggccac
     6301 aaaaaaataa gaaccagcca gaattgctaa aataaaccat agctcccgat aatatggcaa
     6361 aaagatagca ataaataaac caataaggat aattacacca gcggtaaata ggcgaaaacc
     6421 aaaaagatta ttaagaatag actgtttgtc ttctggccgt tcattaatca tctgtgccgt
     6481 taccattgtt agtcctaaat ccgctaagac agcaaacata cctaaaaaat tattcaccgc
     6541 ggtataacga ccaaaccctt ctactcccaa ataacgagct attaaggcta cccctaatac
     6601 tccaagaata atagatatac ctttgccaat gatttggact atattgttat aggcaaattt
     6661 tctagctagt gacatgctgt tgttttttga ccattcatta gatataataa ctatatcaca
     6721 atatcttaaa aaaccaacta tgtctaaaac tcatttctta gctagcggct ttatttcatt
     6781 agctatcctt ctttttactg cctggcctac tttaactgcg gctcaaactt tttcgccggg
     6841 atcacaggct ggcttaacaa ccattggcaa taaagcctat ggcacccctg gttctcccca
     6901 agatatccgt atattaatag caaaaattgt tcagataact ttaggattat tgggtaccat
     6961 tctaatagtc cttataattg tcgctggtgt ccaatatatg acagctggtg gcaaccaaac
     7021 taaagtagag agcgctctta agcatcttac aaatgctctc atcggtttaa ttattgtcct
     7081 tgtttcttac gctattacgg tattcattat ccaacaatta aatttggcga ttatagggcg
     7141 gcaatggacc taaataattc tcttttcgcc ctacctatta tttactcacc ctcctggaat
     7201 agtaggtaaa ccccgtattt ctcctatatt tctttaagga aggccgtgcc cctacgtgcc
     7261 tctaatagac ttaatggtaa aatcaataat taagtaagaa ctaaaaataa tgactagtcc
     7321 aatgagggta taaacaataa tggatttact tttagtaatc ttactttcat ttccccctga
     7381 tataatcata cttattcccc cataaactaa caataatagg gccactgcac caataatacc
     7441 taaaactcta ccaataacct ggcccaaata agcactaccc tctaaaatat tatctaaccc
     7501 attgataggg ttactaaggc tcactggatc agcggcttta gctgaaggtg ttataagggt
     7561 ggttgctaaa agacaaaaat aaataaaaaa tttattcagc ataaccatta aggattagtg
     7621 gtaagaacat caaacacaga cttcaaaata atatatgaac taaaaatgat tgccaaacca
     7681 atagcagtcc aggttatcat ttttttagct ttggtaatag tctgttcttt accaccagct
     7741 gtcatccaca taatgccagc taccaccatc ataacaaggg ttaaagtacc agttaaacca
     7801 agcagactct taataatatt gccaataact gtattaacat tagcagtcaa aagactatta
     7861 aatttaggga gcgcactctc gtcgaggggc ttactctcgg tttgattagt ttgcgattgg
     7921 tctaatgtag tcgggggtat tgcaactagt ggcatataac agcacttctt tccaggagga
     7981 caatgtttgt cgccctttcc atcatggcta ggataaggat cagtacaaat atccaaacac
     8041 cgagctgcgc ctttggtttt taaatcatga atagtacagg taccacagca tttatcacca
     8101 gcactctcac tgctattttt acaacccaga tcaccaccag gataaggaac catataaata
     8161 atatcatcag tacaagtttt caggcacttg gctgtattcg taccaggaac agtctcagca
     8221 cacgttgtta cggctaaagc acttggtgca gcaaccaaca aagcacagcc aaacaaaata
     8281 atgccaaaaa aatatcgtga tttagtcata atataaaaat agacttttta tattataaca
     8341 aaccactaaa taaaaaacaa tttttgtagt ccctagggga atcgaacccc tgttatcagg
     8401 atgaaaacct ggtgtcctga ccactagacg aagggaccat taactaagct cattataacc
     8461 aaagagcttg ggctgtcaag ctaaagcttg aatcagctac caattattag taaaatgatg
     8521 acatgaaaat tttagctatt gaaactagtt gcgacgaaac agcggcggca gtggctcatt
     8581 ctactgctaa ccatattatt attgacagca atattgtagc ttctcaaata gacattcacc
     8641 aagtctttgg tggtgtggtt ccagaaatag cggctcggga acatgttaaa caaattttac
     8701 ccgtcattga gcaagctcta cattctgctg gactaaaacg cttagaagct agccacaacc
     8761 tggatggttt agcggtcacc gttggaccag gtttaatgac ttctcttctg gttggagtag
     8821 aaactgctaa aacattagcc ctggcttgga atttgcccct tatacccgtt aatcatattg
     8881 ccgggcacac aaatgccaat tttattgacc gtgatttaag tagtataaag ttcccccttc
     8941 ttactttaac tgctagcggt ggtcacacta atttactctt aatgcataac cccttacatt
     9001 ataaaattat cgggcaaact atggatgacg ccgctggcga agcttttgat aaagctgcta
     9061 agttactagg tttaggctat cccggggggc caaaaataag tatgatggct aaaaaattcg
     9121 ctgagcagca ccaagaaacg acaattcaac taccacgacc aatgttaaaa aggacagatt
     9181 ataatttctc tttttctggt ctcaaaactg ccttacttta taaagtaagg gaagacctta
     9241 cttggcaaga ccacgtagct gaatatgcct atgtctttga aaaaacagtg gttgaaactc
     9301 tagttagaaa gactattaag gcagctagcg acctcaaagt taagactgtt gctctagctg
     9361 gtggtgtttc ggctaatgaa tgtctaagat ccaacttaaa ggccacctta gaacaaaaat
     9421 taccccaagt tgatttactt ttaccaaata tggcctatac tacagataat gccgcgatga
     9481 ttgctacagc tggactactt aaaatgcaag ctggcttaac tcaagatcta gaacaaatta
     9541 ttgcccaacc aaattattca ttaaaataat attaaaacta tgaccagcga agatatcaaa
     9601 aagctagtag aaagcttaaa aaatgaagat ctttatactt ttgagcaacg gtgtaaagaa
     9661 ttcttctata accatagcta tgaatttgct agtattgacc agaataaccg tgacttcatt
     9721 attaatttct taaaaaatca tcgtaatgac gtagtcagtt atggtggtat tcctggctat
     9781 aaactagata atgaacttta taaaattcat ggccaattat ctgaatatgg ccttagtgag
     9841 ttagattaca acaattttaa aaaaataact aattattttc gttgctaatt agctaataat
     9901 tttaaaatct catcacgttt agtggtcatt atttctggtg ttttagcttc tagactaaat
     9961 cttaatactg gctcagtatt agagggtcga atattaaacc aaaaatcagg ataagtaacc
    10021 gttaaaccat ctagataatc aagttgacca tcgctataac gctcagcaat tagctttatt
    10081 ttagccgctg ggtcactaac tcgtcgattg atttcacctg aattataata tttctggtag
    10141 ggcttaataa gctcagccaa agtctcatga gtcttggata gagcttgaag taatttaata
    10201 ataataacgc caggtacctc ataacagcca tgttccatat ttaagaaaaa atggcccgag
    10261 gattcagcag caaaataggc ccctgactct aacgttgcct ccttaataaa agaatgacca
    10321 accttagtta ccaatggctg accaccatta gcaataatag tttcaggagt aattttacct
    10381 ggcctgatgt cataagcaat agtggcccct ggccgttctt ctaaaaatat tttagccatt
    10441 agagccctaa gaatacctgg ctccactggc cgtccaagat tatccacaaa aaatacccga
    10501 tcagcatcac catcagtagc aatgcctaaa tcagcttgct gacccagaac catttgacaa
    10561 agtggctcta tattctcagg ttttaatgga tcagactcat gactaggaaa agaaccatct
    10621 agggcccagt tcatccgaat taaggaaata gtggaataag aagctaataa tttttctaaa
    10681 taaggcccac ccatgccatt agccgggtca gccactatct tgaatggctt aagtggctga
    10741 aaacctgcta tagctaattc atatgctaca tgctggtcaa gaatattatc tatcttccga
    10801 atagttccta acctcttagc tggagcaagc ttattattaa acactaaatt ttttaactca
    10861 gtaaggccag tgctttcact gactggtcga gcccggtcac ggacaatttt aaagccatta
    10921 taatctttgg ggttatgcga ggcagtaatc ataaggccgc catcggctga ctcattagcc
    10981 actgcaaaat aaaaactagg agttgaacat aatccaatat ctataacttc taccccacca
    11041 tctaccaggc cgcgagataa agcttggtgc aggccaggag aagataagcg catatcatga
    11101 ccaataacta ctgataagct atcagtagat aactcttgac gacgccactg agaataggct
    11161 aaccctaaat cataagctaa agtttcatca aaatcttgac cataaatacc acgaatatcg
    11221 taagccttaa aaacactagg ttgagtctgc atattaatga ttggccttct tagtactatg
    11281 agctttaaaa atagtctgtt ctattgctcc taccccccac caagtagcaa gcatagttat
    11341 tatctgccct ataaatggta tccaaccaat agctactata atagttgccc ctaataaaaa
    11401 tggccagaga tgatttttgg ctttaggaat aattttcaaa cctaaccaat agctcggcac
    11461 cagacaccct atgtaaatca agaaacacca gatagctaaa gcaattaaag ctaggggtat
    11521 gccaataata gtaaaaccag cggctatagc tattatgggc aaaataaata gagtaagaaa
    11581 gccataaaaa cagactagac cagctttatg tttaccagtt tccaccaatc tttcccctaa
    11641 cttaggccat aagctataga gcaccagggc agtaacaagg agcataaaca aatgcaagaa
    11701 ccttgaccac caccaaccaa ccatagcggc tcgtttttcc tgagcggttg ctactttatg
    11761 aaccacttca ccattaatat gagctccact atctatttgg gctgactttt ctgcccgata
    11821 acttaaacct gcaccaatat tagcggtggg gtcaacaatt aattgtgagg ctcctctttg
    11881 tatctctttt aaatctatgg gtgcctcata atgaccagct aatttagcct gaccatataa
    11941 tccatgaacc gcctttctat aaatcccacg actttctatt aataaattgg ctaacaaggt
    12001 ttctccttta atctcactat tatcacccaa taataactga gaaccagata atatagcgtt
    12061 gcggccaatt tgactatcta tattaagatt gctgccaaat agccggacgc taccacctac
    12121 tggcgcctta atattaagat tttccgccgc acaaataaca tcacccgcca ctggctgatt
    12181 aataaataca tcacccccta aacaaatcag gtcatctggc aatggtgtat ctatagtgat
    12241 ctgctgacca agagcataag ttaccccatt taattgatca gctggaccga aggtaacctc
    12301 attgcctgtt cgaccaacaa aagccttagt agtaaaagga attatcaata ctcctaaaga
    12361 caataaccat ataaataatt gccttgtttt taccatataa ttatgattaa atataaaaga
    12421 taaaaatatt attaaatatt atatcattag aagtgatgaa tatcaaagaa aaacatcaaa
    12481 aggaggaaaa aattatccaa agcctagtag ctaaacagcc ccaaaatgtt caagcattag
    12541 cccactggaa acgccagctg tcccgaataa ctgggcaacc aattattagc aataatcagc
    12601 tattagctat ctattttaag ctaacacctc cagataaaat tgacccaaac ttggtccaac
    12661 ttctcaaaaa acgagctgtc cgcactatgt ctggcgtagc cccagtggcc attttaacta
    12721 aaccctatcc atgccctggc cattgccgtt attgtcccca gcaagttaat gtaccgacca
    12781 gctatttagc caatgaacct gcagttatgc gggcccgcca ttgccattat gaccccataa
    12841 aacaaatcca ttatcgtctc cgagctctca ccgccaatgg acatcagcca accaaaatag
    12901 aattaattat tattggtggc acctggtcat atttacctaa aaaatatcaa tactggtata
    12961 ttgctaattg ttatgccgcc gccaatactt atggaaaaac tgcccggcgc taccggcaaa
    13021 acttaagttt agatcaatta aaaaaagcta tagccaaaga acaaacaatt aatgaatcaa
    13081 ctcgctatcg aattgtcggg ttaactctag aaactaggcc ggattgttta acccctcaag
    13141 aattaacgcg cttacgagaa tttggctgta ctagagtaga aatcggtgta caaattgttg
    13201 atgaccatat tcttcgactt aatgaacgaa cgactacagt ccaagacatt gctcaagcta
    13261 ctcaattatt acgtgattgg ggttttaaaa ttacctatca tattatgcca gccttaccag
    13321 gctctaaccc taaccatgat attgccatgt ataaaaaatt atttactgat ccgcgttttc
    13381 agcccgatca aataaaattt tatcccacgg tagtagtaaa aggtagtaaa ctatatgaag
    13441 attggctaaa tggtctatat aaaccatata gtgatagtga gctaataagg gttatcaagg
    13501 agtgcaaggc agccacacca ccatatgtta gaattgtaag attaattcga gatattccca
    13561 aagagtcaat cgaagcaggc aataaaatta ctaatctacg acaaattatc caaaagcaag
    13621 gagttcagtg ccagtgcatc cgctgtcggg aagttaaaga taaagctgtt gactggtcta
    13681 atctccaatt aataatccgc cagtatatag cgggagatgg tgaagaatat tttttaagct
    13741 gggaaagtcc tgaccaatct accatttttg gcttttgccg gctacgctta cctacaaaac
    13801 cagctagtaa tccttatttg agtgactgcg ccctaattag agaactccat gtttatggcc
    13861 tattacaacc cttgggtagt caagggcaaa tccagcatcg gggactggga gaaaaactat
    13921 taattcatgc tgaaaaaatt gccaaagagc atcactattc taaaatagcc atcatctctg
    13981 gagttggtgt ccgtaattat taccgtaaat ttggctatta tctcaatcat acttatttag
    14041 tcaaagctgt gatatagtga taaatataaa aattaatata aaaaatatgt ttaaccccga
    14101 acaatttcta cgaaacttgt cagagcgaca agaaaagaga aaaacactaa ataataaaga
    14161 atacctccaa gaactttcaa taaaagcaca acagatgaac aatattaacc gaattgatcc
    14221 ttatcaattt attaacctat atggacggga ggctgttatg gccgacctta agtgggttaa
    14281 gagtcgacaa gcttacttcc gaacccatga ttctgaaacc gaaacttggc ataaagaagt
    14341 agctaatatt ctagaagcca ttttacatga gcaaatcgag cagaatgact attttggtga
    14401 aaatgtttgg acgattaaaa cctgtgacta tgacgactac cgcaaccaca ttgattctct
    14461 tttagaaata cgccacccag aacgtcgctt tgctaattat tctggattgg ccttagatgt
    14521 aacctcggct agccgtttag aggcactggc aaaaaaaata gaacgaatat tcaaccacat
    14581 aaaaaggggg cagttagcta aaataaaata tttccaatct gattttctta atatccgtgg
    14641 agaaaaaagt aatattcctc ttttcgttgt tggctgtgac gtccatcata ctgaagaatt
    14701 agccaaactc tggggcgatg gccgggcaag aattttagct gagcatccca tccaaattgc
    14761 tttgctttat caaattaccc gccaagccca aatttattgg gattatgcta agcgcaataa
    14821 tcaaaaagaa gtggctgatc aatacgaaga aattctaaag gaatttgagg ctatcactca
    14881 agaacgtcaa accattatta ctgatattaa aaatcgacca gagagcaaag aattcttgca
    14941 gaccgatatt atgctcaaca atttagaaaa aattctaagt gaatatgata aatattatca
    15001 aagaccaaga agtactacgt tttatagccg aggaaagtaa acgacagatt gacgagcttg
    15061 aacttattgc ttcagaaaat tatgtctccc cagcagtact agctgcttta gctaccccac
    15121 tggtcaataa atatagcgaa ggctaccctg gacaccgcta ttacggcggc aatcacctta
    15181 ttgatgaaat agaaaattta gctattgaac gagctaaaca attatttcaa gctgaacatg
    15241 taaatgtcca gccgttatca gggtcacctg ccaatctggc cgtgtatcta gccttactac
    15301 aaccaggcga cactgtctta agccttcgtc tagaccatgg tggccatcta tctcatggcc
    15361 acccagtcaa tatttcaggg cgcctattta attttgtcca ctatgaagtt gatccccaaa
    15421 ctggctgtct caatatggac caaattagaa aaatagctaa agaagtaagg cctagactta
    15481 ttatagctgg ttttagcgcc tatagccgag aaattgattg ggcaggcttt caagatattg
    15541 cttcagaggt cggcgccttt accctggccg atatctccca tacagctggc ctcattgctg
    15601 gtggtcaact aataaaccct acccccttgt ttgacgtagt taccactacg actcataaaa
    15661 ctttacgcgg accacgaggc gcaataatta tgtgccagga aaaattagct aaaaaaattg
    15721 accaggcagt ctttcctggc ctgcaaggcg gacctcatga taacgttacc gccgccaaag
    15781 ctattgccct aggagaagct ctagaaccaa gctttaaaaa atatgctcat caggttattg
    15841 tcaatgctca atttttagct aaaaagttac aagaaaaagg atatactata gtctcgggcg
    15901 gcacagacaa ccatctaatg gtgattgatt taagaccaca gcaaattacc ggcagggaag
    15961 cagaaattgc cctcgaaaaa gccggcctat cagtctcacg ctctactatc cccggcgatt
    16021 cccgaccgcc ttttaaccca tcaggacttc gtttaggtat ggccgccgtc actaccagag
    16081 ggtttaagga gcaagaaatg gaacaaatag ctaattggtt tgaccaagct ctaaataaac
    16141 gagacagcaa gccagcattg gctgaaatta aacaacaagt atctaccctg ggtcgccggt
    16201 tacctccacc ccagtataat taaatctatg ccacaacagc ttattaatgg ccacgaatta
    16261 gccgaagaaa ttaaagactc tattgccaat tttgtctata gcctccaagg accacgtcct
    16321 aacctggcta ttgttttagt tggcaatcgt cctgattctg aactttatgt tcgtcttaag
    16381 caacaagaag ctaagaaggt ggggattgat actcatttat accgctgccc tgaagatatt
    16441 aaagaacaag aattaatagc tatcattaac tttttaaata atgaccccct cattgatggc
    16501 ctcctagttc aattaccgct acctaaacat cttaatcctg ataacattgt taatattatc
    16561 agggcggata aagacgttga tggttttaca cctcttaatc ttcaaaagct actaaacaat
    16621 gacccctcag ccatcatacc tccagttttt ggggtcatat taagaattat cattgaaaga
    16681 cagctagatt taactaacaa aaaagcagtt attttaggta attcaccaat ttttagtgat
    16741 aacttagcct tacttcttaa aagacagggg gcaacggtaa gtattttcca tgaggtaaat
    16801 aaaactgcta gtcaagccat agaagaagcc aatctcctga tatcggcttt aggtcaaccc
    16861 ctcatagtaa aacccaaaga gttaccagaa aattatctaa ttattgatat cggtattacc
    16921 aaaaaagacc ataaagtcta cggagatgtg gacttaaaat ctataaaccc aacgatcaat
    16981 ggctgggcta ccccggtccc tggtggtgtt gggccactta ctattgctat ggctctatat
    17041 aataccctat tactttatca acaacatcat gctcagtcac gagacaataa ataatgtttt
    17101 acaaacttat tttcatttta ctcaattgcg acccggccaa gagtcagcta tcattagcgc
    17161 tattaaagga caagatacga tggtaattat gcctaccggc ggcggaaaat cgctctgttt
    17221 tcaattacca gccctcatct tacccggtat aaccatggtc atttcacccc taatcgctct
    17281 aatgaaagac caggttgatc accttaatgc cttaggtata ccagcgactt tcattaatag
    17341 ctctctaaca gctgatgagg ccaaagagcg tttgggggaa gcactaaata aaaaatatcg
    17401 tcttatttat atcgccccgg aacgtttcta taatcaatct ttttttaaaa cagtccaaca
    17461 actacccatt agtctagtcg cggttgatga agctcactgt attagtcaat ggggccatga
    17521 ttttcgacct agctatctgc aacttaaaaa atttattacc gccctaggac gaccgcccat
    17581 tatggctcta acggctacag ccacaccaga agtccgggaa gatattatta aacaactaga
    17641 cctacatcaa ccccaagaaa ttattactgg ctttgctcgc cctaacttaa gttttgaggt
    17701 tatcagtact gctgatagcc aaaaaaagga aatcgttctc gagactattc aaaaacagcc
    17761 tgaggctagt ggcattgttt atgtcagcac tcgtcagcgg gctgatgaac tagtgcgtta
    17821 tcttcaagat tatcatattg cagccgtaga atatcatgct gggcttaaca ccgctgaacg
    17881 tcaaaaatta caaaatacct ttatgagtgg gcgggctaaa gtaattgttg ctactaatgc
    17941 ttttggacta ggtattaata aaccggattt acgttttgtt attcattatg atatgccggg
    18001 tactattgaa gcctattacc aagaagctgg ccgagctggg cgagatggcc agcccagtct
    18061 ctgtctttta ctgtatagtc cccgggatcg ttatcttcaa gaattcttta ttgccggcga
    18121 taacccttcc ttagatttaa ttttagatat ctatgaattt ctcactactt atcctaatga
    18181 ccatatttta attacctata gcactattaa aaatgctttg gggctagata ctccagacct
    18241 cgctattggt acggctttaa aaattttaga acgagaaggt tatctggcca aaacctatga
    18301 aaaaaccagt caggcgatga tccagctagc ccctaaaaat attaaactaa tcgatgggca
    18361 aaaggcttta ggccctaaag ctaaaaaatc actagaaatc tggggaaaat tatatgaccg
    18421 ctataatggc aaattaagaa ctggcctcta tataaattta gacactttag ctaacagttt
    18481 gggctataaa aaagattctt tagctagagt cctaaaaaaa ttaaatgacc agggcatcat
    18541 aatatatgaa cctccattta aggggacaga aattacttta ctaaaacgct gtcctcgtga
    18601 agccgtaaat ttggaccgta aagccctaga aaccaaattg gcttttgctc aacataaatt
    18661 agaccaaatg gaaaattatg tttatcatcg tggctgtagg cagcaatata tcttagatta
    18721 ttttggggaa catctcgctg gtaactgcgg tcaatgtgat tggtgtaaag gcaattttaa
    18781 tatccattca tttaactctc gccccaacaa gctgtctcaa cgtacctctt accataaaaa
    18841 tttatataat atccaactat ctactaaatt aacacaatta accactctcg agttatacca
    18901 aaatggcctt aatattcaag aaatagcccg agaacgcgag ttacaatcgg aaactattgt
    18961 ggaacatttt tgctatcttc tagaacaagg gttaataacg gatatgagtc aactcattga
    19021 tgaaaacatt attactatgg tccaagattt attagctcaa aagcctcagc tgacaaccga
    19081 ttccttaaaa actcttaaag aacaactacc tagctatgta gattgggctg acttaaaatt
    19141 aactttagct tacttacaac acaacaaatc ccatgaacca aaaacgagct gattacttta
    19201 ggcaaatagt aaaaaataat tatcaggaga tagcgccaga gtttgctgcc agccggaatc
    19261 ggccattatg gcctaaatta caagaaatta ttaacgacat atctccccag ggaagccttc
    19321 tcgatgttgg ctgtggtaat ggtcggctgt tagatttact agttcagcaa caaatcaatt
    19381 catataccgg cctcgatatt tctagccacc tattagaact agcagcgcaa aaatatggta
    19441 ctagttatgg taatataatg atagaatggg tcgagggtga tttattagaa gaatcactcc
    19501 aatgtcagtt tgataatatc tgctgtattg ccacccttca tcatattcct agcactcaat
    19561 ataggcaatt agccgccaat catttagcta actggctaaa accaggaggt tatttaatta
    19621 ttagtgtctg gaatctatgg cgtcaaccac aatattggcc aaaactaata gcaagctatg
    19681 gacaagcttt gctgcgacaa tgtgaacctg gtgatatttt tttaaattgg ggacaacaag
    19741 tgggagatag ctaccggcgc tattatcatg ccctaaataa aaaggaatta ataaaaacta
    19801 ttactaaaag tggcctcaag ataattcatt atgacaatga cggcctaaat cattatctag
    19861 ttgcccaaaa attaccactc agcggtaatc tgagtatcta aagaccaact ggaagtacca
    19921 cttacttgtt tatctttaat cttaaggggc atctcttcag agtaggctcg cttcactgac
    19981 tgaggaaaat ttaaatctac tgtcaacgaa gtccgctgcc ggcctggctg tttttgtaaa
    20041 agcaaattat aatggcctga agaaatctgc tgagcaatgt ctggtggtaa tttataaacc
    20101 aaggtaataa cttttgtctc ttgcggctca accactaaga atccgccaaa aacagttttg
    20161 tctaaatctt cactggcagt cgctggatcc ttaaacccac tttggctaac tagctgacta
    20221 cctcgtggca cataaattct cgtgtagctt tgatagcgcg tagtacgcca gtcaaaacca
    20281 ccattatgct tataggttaa aattaattct gcaaaccaat catcatcgtt gttgtatagc
    20341 ttgtaattta tttgtttatc tacaactgca tcagttttat aggcagctaa attagcatca
    20401 ataatccata aataatcacc actatagcta cgagcctgac cggcccaatt agcttttata
    20461 agccattgct caattactgg gtcataacta aaaagtaaaa tatttttttc ctttaaattc
    20521 ttggtaataa ttttagctat ttctggtaaa cggctactag gcaaagccat taattgctct
    20581 ttgagctcct taatgacatc attgattact tctttacggt tccaagaatt aacgccatat
    20641 ttgtcataag acatttctac ccgatattgt agtaaatcaa caaaattttg ctgattatat
    20701 atttgccctt ccacggtaat gggcccgaca attcctaata aatcctccac tacggcggga
    20761 gtaatagcaa taactccatc aattttggga gtagaactta ctttattaat gacttttatc
    20821 tgttccccat aaaaataacc aatctggcga gcggcggtag gaaaatccgg cgaccagtta
    20881 gcatcacgca aaaaccattg cttgacccct aaatatttat ttaactcttt gggtggggcc
    20941 ggcttaaaat aatcttgagc tggcatatct agatgataac tatcatgaac ctctaaatta
    21001 gtaatgcgac cattttctaa ttctaatagc ccataattgc caataaaacc gccagttggt
    21061 cgtaattcat cattattttg taatactaat aaaaaacgcc catcacttaa ttgcttggcc
    21121 aaataaggag ctaattgcaa ccatggtaga tactcagcta gaagctcttt accgtcttcc
    21181 agcgctttaa gcgcttgttt agcaggagca ttaagttcac taagaccagg cccaaaattg
    21241 actgctgata aactattctc tatctcatga agactcacta aggtatttaa aattaaagcc
    21301 ggtgactgct gaatttggct taaagcaatt ggcaaactgc tagttgttag ctgattaaat
    21361 gataattgag ataaatcagc taaccattgc tgagcttgcc ctaacagttc caaaatagta
    21421 ctacaagcga tatcagccgc tttagaaagg ttatgggcac tagtagcttg ctccctatac
    21481 catggtatat gagaccacaa aggatgctgg accaaaatta ggcttaaact gcgagcccga
    21541 gcaaaagact cctgagcttg ccccatagct tcttgggctt gtgaccaatc acctcttttt
    21601 agagcttgcc ctccagtagt aagttgagtc ttgccctcta ttcctgcttg ataggccccc
    21661 caggcggtat aacctaatcg cgctacatca ataataaaaa ttaccaagat gataattgct
    21721 atagtccaaa tgaagcgacg tttagacata aatatcaaat tattttctat attctatatt
    21781 acaatagcta aaataaaaac agggtctaag tgaccttgtt ttatatactc gatttattcc
    21841 tcttttttat cagcatctga ctctgaaggc actgccggct ctaatccggg tgccggagaa
    21901 tgatcagtgt gatcgttctc tttattctta tttttatttt gatctactgg ttgttgtctc
    21961 ctaattttaa taatattgcc agtaacaatt ttatggccaa ccaataaatt attaactgtg
    22021 atagtcattt gcgctccttc atttaaccaa taattaattc tttccgcttt aacctgtaaa
    22081 tctttggtct tagggttata gctacctaaa ttttctaaaa tacgaccata gggatcacga
    22141 ccagcctcag aaataattaa cctataaaca ggagatttct ttttacctat acgagcaagt
    22201 ttaattttaa gcataaaact acacctaaat tttagaataa ataaatgagc tctgacggta
    22261 ggctgattat atcttaataa aactagtttt gtcaagcaac cgtatcagtc cgccacattt
    22321 tggacaagca cctcactaag cctcacagtc gctcttcttg gtctttagca ttagcactgc
    22381 tagctctaaa tcctttgttg ggcttcttta gtaactatat aattatatct attggcatgg
    22441 ccatatagtc tagcaaaact ctgtgatact taaatattat ttacatatat tttatcctcc
    22501 tttggagtcc aaaatgtgag tacacttatt cagctgaaaa tagcgaataa aaaaaggaac
    22561 cgcccagacg agccgtgcgg ttcaagttta agaagactaa agttggttca gtcttttgtt
    22621 agttaatgga ttttgatgac cttggtggtc atcatttgat tctcggcggt ggctctaata
    22681 aaatagacac cgtcagccac ctgtttgccc tgactatttc tgccatccca aactattgac
    22741 tgggtcttgc tattattggt caggctttga atcttctgac ctttaatatt aaaaacctca
    22801 atcttagtgg ggatgttgcc aacaaactta atggtcgtac tggcagcgaa gggattgggg
    22861 taacagccaa cggacatcag cgttacagga atcacagggt cgtcaacggc ggtgctgtaa
    22921 tcaccagtca aacggctaat agcttgccga aattgaacgc cgtccggcat aacaaaagta
    22981 accggctgcc ggttcaataa ctcgactatg ggctcttcag cgtcttgctg aaaacgcaga
    23041 agattattcc catctaaaag aattgccgcc gaccaggggg caccgtccgg atatatgcct
    23101 tccacagcga ataagttgtc tccgttttgg ggaatagtaa tggtctgacc actaatctcc
    23161 ggcgacaact gattataccc ccaatcgtca gtaaacaatt caccaatatg gaggttctgc
    23221 accaacgggt tgtcagggtt gactacccaa aggttttgta gataggcatt aatctgccca
    23281 ccagcaaccc agggtaccgc cacacatcca gccgcataat ttagcccggc tgtgtagaga
    23341 taatcttgga agctaagccc aataaaccca ctggcggccg ccaatgatag agcagtatcg
    23401 gtatcgttaa ccactccatc attattaaca tcgccgcgaa gacggttgtg ccgcttaata
    23461 acggcaacac tcgcctccga cagaatatta tcaaaaaaac ccgcattggc cccccaacta
    23521 atttccccat tccagtaaat atagccagtg gggtttacaa tagaagaata agggaccacc
    23581 atctgcacaa tgggctttgt actagaatag taccagctat tacccatcca tgcccccggc
    23641 atgtccgcta cccaggccgc tctccaccac tcctcatcga gctggtagat aaacaatcct
    23701 tcctcttgcc aaaacatata gcaatcggaa taactgttaa aaactaaggt gcccccatcg
    23761 agaccataat gtcccagatt aaacaccata gcctccacct ctggcgcctg gcagaggaat
    23821 gtaatgttaa tatcctgccc cggcaacacc caaagggtgt caggggttgg actgccggcg
    23881 ttcgtattta taaacaaacc aaatgccgga acacaaacca atatgatgac cacgaatgtt
    23941 aataaagttc tctccatgat gagaacctcc tttttctttt gttgttaaga ttgctatcta
    24001 aacaatgaca caagaaacct attatgtcaa gactattatc agcccaccac attttagaca
    24061 agcatctccc caagcctcac agtcgctatt cttggtcttt accattaaca ctgctagctc
    24121 taatatcctt atcctttatt gggcttcttt agcaactatt tacacatgac catatagtct
    24181 agcaaacact aggcaataat aatgcttaaa tattatttac tattttgccc tcctttggag
    24241 tccaaaatgt gagtacactt attcaggaaa tgagcgggta acgggaatcg aacccgtatc
    24301 ttcagcttgg aaggctgaca taaaagccat tatactatac ccgcaggtgg ccaaaatgca
    24361 gtagtctata ttattaattt ttaatcaaaa aacaataaag actctgccat tccagccaga
    24421 gtcgggatgc cgagatttga actcgggatc tcctgctccc aaagcaggcg ccttaaacca
    24481 gctgggccac accccgctat ttatcaacca tcaatatctt atttatcaga atgttctgct
    24541 gcaagtctct cataataggc tcgcctttct tctacaaatt tctcagcctc ttcctgattt
    24601 tcaaaaactg ccactaaatt agcatgtcct tctctactgc cccgccaatt accggtaagg
    24661 ttatcttctg gagtagtata gacttcacca gccgcttgat cttcaaatac agcaaatacc
    24721 ttaggtcctc ttaattcttg aatggctttt tctaactctt cttgagagtc atataaacca
    24781 acctcacgat aatgatcatc ctgggtacga tagccactat tttcccaatc agctaattct
    24841 tcttgtgtcc attcttttac ataataagca ccagctgggg catcataaag cagctgatat
    24901 ttcttctgag aagagacctg ctctttagtt tctgatggcg acgtataatt ttcaaattta
    24961 ctagacataa tttttcttta tatgtggtgc cccgggtcag aatcgaactg acgacacaag
    25021 gattttcagt cctatgctct a
//
LOCUS       DAOOIS010000006        22745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_2307_length_22745_cov_43.343940, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000006 DAOOIS010000000
VERSION     DAOOIS010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22745
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2307_length_22745_cov_43.343940"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..862
                     /gene="lgt"
                     /locus_tag="PK960_01350"
     CDS             74..862
                     /gene="lgt"
                     /locus_tag="PK960_01350"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPD07850.1"
                     /translation="MYWLNNPPSAIIFSIGPVNFYWYGLIMVLAMVIGYYLTWSLAKK
                     HNWKSSDLLDLAFNVIISGVIGARLYHVIIHWPDYSHNLIDIVKIWEGGLAIHGAIIG
                     GGLALLLTCHKRHWPSWQVASWLTPGLALGQAIGRWGNFFNQELFGPPTNRPWGIPID
                     VVYRPIEYLTTTHFHPLFLYESIACSLLALILYYLNRHTIKAQYIVATYLIGYGLIRF
                     IIEFWRQDPTLYLLGWRWPQWISCFMILVGLWLIIYPLILHKNP"
     gene            910..1176
                     /gene="rpsT"
                     /locus_tag="PK960_01355"
     CDS             910..1176
                     /gene="rpsT"
                     /locus_tag="PK960_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPD07851.1"
                     /translation="MPNLKSAKKELRKSKRRTTRNAATRQEIKKMVKGSRRAISAKNP
                     QAHDLVKQAAKTLDKAAKKGVIKKNTASRKKSRLQKKLNATKGQ"
     gene            complement(1242..1913)
                     /locus_tag="PK960_01360"
     CDS             complement(1242..1913)
                     /locus_tag="PK960_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07852.1"
                     /translation="MLTFFYGHANIPHHFYTTHACFLVSGSKSFMDYSCCSPYLFSKK
                     RNRQISCWLLGVVIWELLLLPRPILADAKQRPRAQAKSLVFDLTINNIEPSDLANERL
                     SLRLPEKDIIVEEITKTVSESSSSLYIITAYNSSMSQCDASPCITASGFNVCKHNIED
                     TVAANFLPLGTKIKIPDLFGEQIFVVRDRMHPRHHNQIDVWMKEYKKAKQFGVKVARI
                     EIISF"
     gene            complement(1961..3382)
                     /locus_tag="PK960_01365"
     CDS             complement(1961..3382)
                     /locus_tag="PK960_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPD07853.1"
                     /translation="MKRNRIFLMAIFLMAITSLPAQAGPQQLWSITLDLFLTEPEVTN
                     NLVVIGDNLYLLAETLGNVPYLLKLVQVNKTTGEVGWIRNLPSQGLEVVNNQLAVSPD
                     NNLVLAHAGIVHKICPENGQDIWTHDFTNDSPALVCRLCATADYLICLDCFSHLFLVD
                     YSDGHTIGTLFVSRGYYSVDSKGGNTIFMGQRDGLHGYDPNKSVISKIVVDTTATTWD
                     SYQTEWRVEFPEALPRVLLDGSCSSLYVVGTDYSLPSELCSLRYLDASNGNCLWSVLL
                     QSSTFIRGMISSDSDAEAEGIFLYGRHLANPLLLSYSASGYLNWIFSPVLPGGAVDGQ
                     YEDAIWDGDTLILIAPIAEWGSNLLADYLWLSAISTVNGVEDGASVPIVSQATCYPNP
                     FRGSTSLKFKQAVAGPVTVGIYNMKGQLINTLVNAQPFTCGEHAINWDGKNSRGQKVT
                     PGIYLYKITVDHFSLTGKMVLVK"
     gene            3627..4910
                     /locus_tag="PK960_01370"
     CDS             3627..4910
                     /locus_tag="PK960_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPD07854.1"
                     /translation="MNQTKDNEKEFSKLLEKDPIPIPKVGDTVSGRVISTSKSEVKVD
                     INGVMTGVIRGRELYFSTNDYTKLPINTEIEATLIDIDNENGELELSFRHIDEQKSWA
                     LLQKNYADKTNIKVKVLDANRGGLIVALSQLTGFLPVSQLAPNNYPRVSGGDKTKILE
                     KLKTLVGSELEVKIIALDENEKKFIVSEKDAWQDKQKDVISRYKVGSIVEGVITAITG
                     FGLFINFDNDLEGLIHISELAWQRIDDINELYKIGDKIRAEIISIDGSKIFLSAKKLV
                     HDPWRDVAKKYKVGQKVTGTVLKVNPFGLFVELDKDIHGLAHVSQLALKPGQKITEAY
                     TPNSKAEFIIISLEPDNHRLGLASINKKVSRASSSNNPPEDNEEEPKKVNDQSKKDKS
                     QQDKQSPIDKETKNNKTQDAISKKTGKKSIKKAKD"
     gene            4973..6796
                     /gene="ftsH"
                     /locus_tag="PK960_01375"
     CDS             4973..6796
                     /gene="ftsH"
                     /locus_tag="PK960_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPD07855.1"
                     /translation="MKNLFKNFFLVLIIFLTISYLFSLYSSKSTPVEKIGLERLVQEI
                     NNEEIASITVKGDNLSIVLKDNREQTADKEPGESLSQLLHNYGVSPEKITAVNIRVKN
                     DSTATIFLTTVLPLLIPILVFAGLFYFIMRSAQGVNNRALSFGQTTAKEIDRHKNKIT
                     FKDVGGVKEAKEELREVVQFLKQPKKFTDLGAKIPKGILLLGSPGTGKTLLARAVAGE
                     ANVPFFSLSGSEFVEMFVGVGASRVRDLFKKAKKSAPCIVFIDEIDAVGRRRGAGLGG
                     SHDEREQTLNQILVEMDGFEADTNVIVMAATNRPDVLDPALLRPGRFDRQVILDEPDI
                     KDRYEILKIHATNKPLAQDADLHLVAQRTPGFSGADLANVLNEAAILAAKKNKKVIDN
                     EDLLVSIEKVMLGPERKSRLLSDKEKEITAYHEAGHALVAHFLPHTDPVQKISIVARG
                     QAGGYTLKMPERDKHLQSKQEFIEEIAVLLGGYLSEKIVYGEVTTGATSDLRQATGLA
                     RRLVTDFGMSEKLGPRTFGEKEEMIFLGREIHEQRDYSEKVAEQIDQEINNFIHQART
                     MAEEIINNHRQELETISHELITKETIEKDAFEAMVGPKPLA"
     gene            complement(6780..8105)
                     /gene="murD"
                     /locus_tag="PK960_01380"
     CDS             complement(6780..8105)
                     /gene="murD"
                     /locus_tag="PK960_01380"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPD07856.1"
                     /translation="MINLKDYSNKKIAVLGLGTENEALILWLARQSLPLDLSIYDRRE
                     ATLLGDKSEKINHLASNLSIKWQLGRKSYRSLNDYDELWRSPGWPIFCPQVQEAIRHG
                     SYLNSPMNLFFEFCPTKNIIGVTGSKGKGTTASLIYDILKLAGWPVFLGGNIGVPPFA
                     FFDQLNKDSWVVLELSSFQLEDLRFSPHIAVITNLYKEHLAPADPVNPNYHKSFDDYW
                     LAKEHIFLHQTSSDWLVINKKLSNLLDKKLCPGKIKWFGRLTWPSKLIGEHNQENVAA
                     AWAATKIVNVPLKIAKEAVANFKGLEHRLEFVGEKNGVRYYDDSFATNPDSTIIAIKS
                     FREPIILLAGGADKGNDFTSLAKVIISQQVKVVILFEGEASPRFKDSLLSAGYPPQQI
                     LSASSMTEAMNLAKKYAQAGDIVLLSPACASFGLFKNYKERGNLFKQAV"
     gene            complement(8108..10771)
                     /gene="polA"
                     /locus_tag="PK960_01385"
     CDS             complement(8108..10771)
                     /gene="polA"
                     /locus_tag="PK960_01385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPD07857.1"
                     /translation="MPSADKKLVVVDGNAVTYRSFYAIQSGLSSNNQPTNAVYGFFSV
                     LFKVINELSPDYLVVAFDRPEPTFRHELSVDYKAKRIKQPEELYSQIPIIKELLTAIN
                     VPIIELAGYEADDIIGTIVSHTPKSIHNYIVTGDKDAFQLVNDHVSVYWLQRGLQKTV
                     ILDREGVKRILGLEPEQVVDYKSLRGDASDNIMGVPGIGDKTAVSLLQSFGSLDNIQT
                     AVAAGDNIKIKPRWRQLILANQESLDLSQKLASINSNVPIKFSLKEAKFTGFDFILAR
                     QAFLKWQLNSLIPRLEKLSHYSKSRPAVFAISQSRIIEINNVNESTNLAHSWTGKTLA
                     LRPAFDGKRITSLMLAGQNEIYQLVIGKEIQGELFTPSTSRNPILEPWRTILEDKNTK
                     KIIFQAKEVIKFLLTQEIVLEGLSGDPLLANYLLHPGERQISLMDIGRQVGFEWGEKS
                     GSPKDAAFGVLQIMDIHEALIKELTDTYLLKVYNEIEIPLITVLAKMELAGIKINQEV
                     LTNLAQKWQDSLNRLSQEIYNQAGEEFNINSPKQLQDILFKKLALNSKGLDKTKNGPS
                     TDADNLQKIKDQHPIVSLLLEYREISKLLNTYALALPKLISTKDGRLRANFHQAITAT
                     GRLSSSDPNLQNIPIRTEMGRKLRQAFVPEQGYKLISFDYSQIELRIIASLAHEDSMI
                     ANFLAGHDIHAVTAAKINGIPLEDVTEKQRRAAKAVNFGILYGQGPHGLSRATGLSYG
                     AAKDFIEQYFTIYPQIHKYINELLIKARQDGFVTTFWGRRRYLPDLLSPNPTLRRAAE
                     RMAINFPVQGTAADIMKAAMINVDHWLEDNFAWVQARIVLQIHDEILIEASEELVDKI
                     TAAVPPLMTEIIALPVPLVVSTKVGNSWAEL"
     gene            complement(10844..11338)
                     /locus_tag="PK960_01390"
     CDS             complement(10844..11338)
                     /locus_tag="PK960_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07858.1"
                     /translation="MPKSKKFPRWIWLVIAIIIIGGILIWVMKNRSVPNMDELGRDNL
                     YHYRNEELGFSLDLPPEFIYYQVERFNAADYTEIRFFVPTSDPNYVYEVVPSYAKPIV
                     IRVYSEAAWNQLEDDDEGKQAFEKKMTKNGRVYVIQFWDHQPSDWQDKWSDDMKSAIS
                     ESLR"
     gene            complement(11346..11591)
                     /locus_tag="PK960_01395"
     CDS             complement(11346..11591)
                     /locus_tag="PK960_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07859.1"
                     /translation="MDNIIKIVNKKIRQIVISLTVVGILLLILSFLTVWTTFMAKLVM
                     GMVILLLAYAFLSTAYRLWKIQQDIKDVWQIKDTKIK"
     gene            complement(11651..12616)
                     /locus_tag="PK960_01400"
     CDS             complement(11651..12616)
                     /locus_tag="PK960_01400"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPD07860.1"
                     /translation="MDKVEALKAWSLMREAKNWLIATHFNPDGDALGSVNTFLELAAA
                     MNKPVMAYCASAPDDKFGFLSRINQLANKITKEQFDEFDLIITVDCATASRSKLKEWL
                     FSRSANQIVIEFDHHERQEDYADISFKESGAAATVEIIYNFLLANDWPINAEMAKSIL
                     TGLLTDTGNFLYSNTNDKIAKIAAKMMLIGARLPKIVRQTVTNKNLAVMHLWGLVLSR
                     LKINYEYLVAIATVKQKELSEFGLGVDDLTGLTGLMSGLQGVNGSLLLIETENGQIKG
                     SLRSHLTNIDMSELANFLGGGGHRQAAGFCVNGSLVEGGGQVIVS"
     gene            complement(12623..12955)
                     /locus_tag="PK960_01405"
     CDS             complement(12623..12955)
                     /locus_tag="PK960_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HPD07861.1"
                     /translation="MIHNFKRANKTLQREIADLVNKLIEFPGGLLSITYTSLNADCLE
                     MKVGVSVLPINLRGTALRKLRKKSAEIAKFLAKKYQLPRIPRIIWLIDDSAEEADHLN
                     YLINKLSS"
     gene            complement(12959..14926)
                     /gene="infB"
                     /locus_tag="PK960_01410"
     CDS             complement(12959..14926)
                     /gene="infB"
                     /locus_tag="PK960_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPD07862.1"
                     /translation="MNITELARRLRVSPQELRDTLPKIGFDIGQKAIKIDQYLAQRII
                     REWPRLTRDLKNREERERQQEEAVATARAERRVVKIGPVITVKDLANVFGLPVNRILT
                     ELMQNGIFASLNEKIDFDTATIIGSELGIGVVASEEEAMDNDINELKEAENISGSNNL
                     IVRPPIVVVMGHVDHGKTQLLDAIRHTDVVATEAGGITQHIGAYQISRKDKEVTFVDT
                     PGHEAFTAMRKRGAKVADLAILVIAADDGIKPQTIEAYRIIKEAKIPFVVAINKIDKP
                     EANVDRVKQELANELGLVPEEWGGKAIICSVSARYGQGIDELLDTILLLADLNAEARL
                     ADPLAKAVGTIIESHIDKGEGPVATVLVHNGTLKIGDNLMCNQQYYGKVRALKDYRGQ
                     LIQEAGPSTPVKVIGLRATPEIGDIVRVESVEHRNKTKIIAKQENQAIITKVQDDNSF
                     KGTPINLVIKTDVYGSGEAIEESLLKLNNEEVAVRIIYRGLGNITEGDVVRAAANQAK
                     VIGFNVGVNAQVEDLARDKGVLIERYQIIYDLIDKVRQDMQALMKPEIKRINLGQVKV
                     LAIFKQEGMTQIVGGKVIEGTIRADSLLEVVRGGEVIGQGKLVHLQAGRQEVKEVVTD
                     QECGLQIESLEVLKVGDILRIYREDEVFKKM"
     gene            complement(15060..16043)
                     /locus_tag="PK960_01415"
     CDS             complement(15060..16043)
                     /locus_tag="PK960_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07863.1"
                     /translation="MINYKNILKQTWQITIKNRWLWVMGLFAALLSNVNQYTSLLNAF
                     DGSGWSRFLNSLSVYWNYGLSLINISSHNPLLLLLCLLALVLIIGLLFLAVNSQIVLV
                     QKIQHSIKSEGKKSLPLPRVSFWQQIKNNWPIFWPTVGIIVTVKLILLFGLLIISLGM
                     TGAYLIRQPIISVFVYIVLVLLFLVLIWLIGTWGFYWLLLFLSDKKSDWLASAKEAWH
                     ILCENLLASLGIFIIVVLINLVVYLLWIFIMGLLAIPYSLLSLLLIRYLSFSEVPLII
                     IAQILLIAGLMFVVGIVTVFEYSLWLSFIDKLPNKSNKNIIRRVSHAFHRQ"
     gene            complement(16052..16771)
                     /locus_tag="PK960_01420"
     CDS             complement(16052..16771)
                     /locus_tag="PK960_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HPD07864.1"
                     /translation="MKHLFTILFYQPVLNLLVWFYNILPGHDMGVVIIALTILVKLIL
                     WPLSQKSLKSQQAINRLQPKIKEIQNKYKNDKEKQGQALMDLYRQEKVNPASSCLPLL
                     IQLPLLWAVFMVFRDGLSNQSLSLVYPFISRPELLKTTFFGIIDLAKPNEILAVATGL
                     VQFFQSKISAHYTMPANTGNGRADNLTAMMSKQMIFMMPIMTVIICWTLPSGLAMYWL
                     FSSLFTIFQQLYWFKKTPKSN"
     gene            complement(16777..17043)
                     /gene="yidD"
                     /locus_tag="PK960_01425"
     CDS             complement(16777..17043)
                     /gene="yidD"
                     /locus_tag="PK960_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPD07865.1"
                     /translation="MIRVIKFLEQIVINLIIAIISGYQKTLSPDHGWFKKYFPYGCCR
                     FRPTCSEYAQAALKKYGLIKGSLISINRILHCHPWSSGGWDPVR"
     gene            complement(17040..17384)
                     /gene="rnpA"
                     /locus_tag="PK960_01430"
     CDS             complement(17040..17384)
                     /gene="rnpA"
                     /locus_tag="PK960_01430"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPD07866.1"
                     /translation="MLKKQNRLTKKKDFDRLFRSAEAKYYGKLLGVRIVANGLDYNRF
                     GFVVSRRYCHLAVERNLIRRRLATIVEENLSKMVGGHDIVIVVIAPWPLLKFIDLRQE
                     LINAWKKLKLIV"
     gene            complement(17405..17542)
                     /gene="rpmH"
                     /locus_tag="PK960_01435"
     CDS             complement(17405..17542)
                     /gene="rpmH"
                     /locus_tag="PK960_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPD07867.1"
                     /translation="MPKRTYQPNNLRAKKKHGFLERMMTKSGRSVLRRRRNKGRQRLT
                     K"
     gene            17728..19113
                     /gene="dnaA"
                     /locus_tag="PK960_01440"
     CDS             17728..19113
                     /gene="dnaA"
                     /locus_tag="PK960_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPD07868.1"
                     /translation="MDHSSLEQIWQAILSEAELNMSKASFTTWFKNSFIFDYQDDILT
                     IGVPNAFTQAWFENKFSKDITKTFYKVTGNKLKGIKYIIKTKPKEAKSNFLAPDPPEE
                     KENKAYKTNPSKDESLCLAAGLNPKYTFETFIVGKHNELAHAASWAAIRQPGETYNPL
                     FIYGGVGLGKTHLLQAIGHEVLKSGKKVLYVTSEKFTNDYVDAVYHGQGNTFKSVYRN
                     VDLLLVDDIQFMAGKDGTQQEFFYTFDQLHRANKQIVITSDRSPRNMKAVEARLITRF
                     EWGMVADIGQPDLETRMAILNSKCQQKNFPLDQSIIQYIATNISNNIRELEGALNRII
                     GFCQFHNCEPDLSIAKQVLKDFFISIQANNLSTPQKILEAVANFYNLNLSELTGSSRK
                     KELVIPRQIAMYLLRTRMDASFPFIGQELGGRDHTTAMHACAKIERDIQNNSKIRQDI
                     EQISQSITSVY"
     gene            19315..20451
                     /gene="dnaN"
                     /locus_tag="PK960_01445"
     CDS             19315..20451
                     /gene="dnaN"
                     /locus_tag="PK960_01445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPD07869.1"
                     /translation="MQLTILQKNLHQGLTIVSHIAEKNLSMPILNNILIKAKDNIITL
                     AATNLEIGITKQIRGKVNKEGGIAIDAKIFSDYIALLPNEKIDIQLINKQDLKINCKK
                     YNTIIKGQNAEDFPVIPQIDKKISLTLAVSDLKLALNQLLFAINPTDNRLELTGVLFD
                     INNKYINLVSTDSFRLAKKEINFHLDFGTLDSEGLKIIVPFKTMQELARIINFSNDEL
                     TEDSDQIQIHIADNQVLFVYQGIEIVSRLIEGHYPDYIQVIPTDFKTEITIDRGEFLR
                     AIKMASIFSANSNAVDLTINADQQEIIIEAVSGQIGSHQAKLAAQINGKDNSIRLNYR
                     YLLDCLNILPTNQVLFKIIDGISACEITLPGSNEYQYIVVPLRK"
     gene            complement(20452..21486)
                     /locus_tag="PK960_01450"
     CDS             complement(20452..21486)
                     /locus_tag="PK960_01450"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07870.1"
                     /translation="MLFFRYGEESFQIQRDYKQQLRELDSNTVASLQSFNIPETDWLE
                     LNNSLNNPSLFQSTKIITLNNLLSGKNSIVENGLINELKKETTIIESPSIYLFINDYN
                     LGMKYLAGKQRPVILGGDGRTKPLNKNEQELFLLLTQKAKLVKYYPKLKGLGAEKFLE
                     QLIKERNIELTPAAKKLLLELTGGNFWQIYHELNKLANYIAGSDHKKVVDADDVNLLV
                     NNTTKHLFEFIEAFCNQKLAQTTKLIEEIFNDEDSIATFLALLNRQVLQFLDIKTRLE
                     AGQSLIEIEKSLNLPKSVSQKLINQASKLKRDSLIQLLDNLTKFNWSTKQSHGNEASL
                     FTLLTIAHAK"
     gene            complement(21496..>22745)
                     /gene="nusA"
                     /locus_tag="PK960_01455"
     CDS             complement(21496..>22745)
                     /gene="nusA"
                     /locus_tag="PK960_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPD07871.1"
                     /translation="ESALAAAYRKDYGDKNQNIKVKFNEDLSKIKVYDVKTVVEDMPL
                     PTFNAPLTLKSLQESTVTIDPEAAKELTTIREDQPPTEIRDENGEIIRKFNPKTEIQL
                     SEARLIKPNAKVGDEIITELPAHTEYGRMAAQTAKQVIIQKLREAERDMIYEDFKDKE
                     KEVISGIVQRREGHNVLVDVGKTVGIIPPQYQIPSENYRPNSRIKVFVEEVRVSNRGP
                     EIILSRTADEILSKVFYLEIPEISNGLVDLKAVAREAGSRSKVAVAANSENVDPIGSC
                     VGQRGSRIQTIINEVGGEKIDIIEYSDDPSKFIANALAPAKIEQVELDYENKQATITV
                     DPEQLSLAIGRDGQNVRLAAKLTGWHLDIVSSADKTNKTPTKETSKKEKTNKTKVISK
                     EKAKKVTKKTSTSASKTKKTAKK"
BASE COUNT         7474 a         4462 c         3629 g         7180 t
ORIGIN      
        1 aatcggcctt ttttctttat taaaaaacct atataataat taaagaagtt cagccttctg
       61 ttaatttata attatgtatt ggctcaataa cccaccctca gctattatct ttagtattgg
      121 cccagtaaat ttttattggt atggcctcat aatggtttta gcgatggtta tagggtacta
      181 tcttacctgg tcattagcta aaaaacataa ctggaaatcc agtgatttat tagacctagc
      241 atttaacgtt atcattagtg gggttattgg tgcccgactt taccatgtca ttatacactg
      301 gcctgattat agccataatc taatagatat tgttaaaatc tgggaaggcg gcctagcaat
      361 tcatggagcc attattggtg gtggcttagc tctactgctt acttgtcata aacgacattg
      421 gccttcctgg caggtcgcta gctggcttac gcctggtttg gcgttgggac aagctatcgg
      481 ccgctggggt aattttttta atcaagaatt gtttggccca cccactaatc gcccctgggg
      541 catacctatt gatgttgtct accgccccat agaatattta accactaccc acttccatcc
      601 tctatttctt tacgagtcca tagcttgtag cctattagcc ctcatcctat actatctaaa
      661 tcgtcacact atcaaagcac aatatattgt agctacttat ttaattggtt atggcctaat
      721 ccgctttatc atagaatttt ggcgacaaga ccctactcta taccttttag gttggcgctg
      781 gccacaatgg ataagttgct tcatgattct tgttggctta tggctaatta tttatccgtt
      841 aatcttgcac aaaaatcctt aatatgataa atttgcttta atgctttatt tattttatta
      901 attccaatta tgcctaatct aaaatcagcc aaaaaagagt tgcgcaaaag caagagacgt
      961 actacccgta atgctgcaac tcgacaggaa attaaaaaaa tggtcaaggg cagtcgccgg
     1021 gctattagtg ccaaaaatcc ccaagcccat gatttagtca aacaagccgc taaaacatta
     1081 gataaggctg ctaaaaaggg ggtaattaaa aagaatacgg ctagcagaaa aaaatctcgc
     1141 ttgcaaaaaa aactaaacgc caccaaaggg caatgaagaa ataaaaattt taaacttata
     1201 actcaaaaca ctccatacct atggagtgtt ttgatataaa attaaaaact tataatttca
     1261 attcttgcca ctttaacacc aaactgttta gcttttttat attctttcat ccacacatca
     1321 atctgattat gatggcgagg atgcattctg tctcgcacca caaaaatttg ctcgccaaat
     1381 aaatctggaa tcttgatctt cgtccctaag ggtaaaaaat tagctgccac cgtatcttca
     1441 atattatgtt tacaaacatt aaaaccacta gcagtaatgc aaggcgaagc atcacactgg
     1501 ctcatactag aattataggc agtaataata taaagactac tactagactc actaactgtt
     1561 ttggtaatct cttctactat aatatccttt tcgggcaaac gaagcgataa tctttcatta
     1621 gctaaatcac ttggctctat attattaata gtcaaatcaa ataccaatga cttggcctga
     1681 gccctgggcc tttgttttgc atcagccaaa attggccttg gcaatagtaa taactcccag
     1741 attactaccc ctagcaacca acaactaatt tgtctattac gttttttact aaacaaataa
     1801 ggactacaac acgaataatc cataaaactt ttagatccag aaactaaaaa acaagcatgg
     1861 gtagtataaa agtgatgggg gatgttagca tggccgtaaa aaaatgtcaa catgtttcat
     1921 taaaaaaccg gtagtttgtt caactaccgg atgttgttag ttattttacc aaaaccattt
     1981 tcccggttag ggaaaaatgg tcaacagtaa ttttatataa ataaatgccg ggggttactt
     2041 tctgtccccg actatttttg ccatcccaat taatggcatg ctcaccacaa gtaaatggct
     2101 gagcattaac tagggtattt attaactgtc ccttcatatt ataaatacca acagtaactg
     2161 gtccagcaac tgcttgttta aatttaaggg acgtactacc ccgaaaagga ttaggatagc
     2221 aagtagcctg actaacaatg gggacactag ctccatcttc cacgccatta acagtagaaa
     2281 tagccgacaa ccacaagtag tcagcaagta ggttcgaccc ccactcggct atcggggcaa
     2341 taagtatcaa agtatcacca tcccagatgg catcttcgta ttgaccatct acagccccac
     2401 ctggtaatac tggactaaat atccagttca gataaccaga ggcactataa ctaagcagca
     2461 gtggattcgc tagatgtctt ccgtataaaa aaattccttc agcctctgcg tcactatcac
     2521 tgctgatcat acctcttatg aaagtagaac tctgcaatag tactgaccaa aggcaattac
     2581 catttgaggc atctaaatag cgcagtgaac acaactcact gggtaggcta taatcagttc
     2641 caacaacata tagggaactg cagctgccat ctagtaatac acgaggaagc gcttcaggaa
     2701 actctacgcg ccactctgtc tgatagctgt cccatgtggt agcagtggta tccaccacta
     2761 ttttacttat aacgctctta tttgggtcat aaccatgtag accgtctctt tgacccataa
     2821 aaatagtatt tccacccttg ctatcaacac tataataccc ccgagataca aacaaggtac
     2881 caatagtatg accatcggaa tagtccacaa gaaataaatg agaaaaacaa tccaaacaga
     2941 tcaaataatc agcagtagca cacaatcggc aaacaagagc cggtgaatca ttagtaaaat
     3001 catgagtcca tatgtcctgc ccgttttccg gacatatttt gtgaacaatg ccagcatgag
     3061 ccaacacaag attattatcg gggctcaccg ctaactgatt attgaccacc tccagcccct
     3121 gcgagggcag atttcttatc cagccgactt ctcctgtcgt cttattaacc tggaccaact
     3181 ttaaaagata tgggacattg cccaatgtct ctgctaatag atataggtta tcacctatca
     3241 ccactagatt attggtaacc tcaggctcgg tcagaaaaag atctagggta atcgaccata
     3301 attgctgggg cccagcttga gctggcagag acgtgatagc cattaaaaaa atggccatta
     3361 aaaagatgcg gttccttttc atggaacacc tcctttattt ttagtttaat ttttaaagaa
     3421 cacctaattc cattaacagc attatagcaa aaccatcaac atcactattt acctatctac
     3481 ttgccttttt taaaaaaatc ttttaagatg tttttatctt acacctattt aaaaactttt
     3541 ggcaaggtga accaaaagac ttcagcaaaa attgacaatt ttgctagagg ttaatctcct
     3601 actaggtgtt agcctagtta ttttttatga accaaacaaa ggacaatgag aaagaattta
     3661 gtaaattatt agaaaaagac ccaataccca tccctaaagt cggtgatacg gtttctggac
     3721 gagttatttc tacctctaaa tctgaagtca aggtagatat caatggtgtg atgaccggtg
     3781 tcattcgagg acgtgaactc tactttagta ctaatgacta taccaaactg ccaattaata
     3841 cagaaattga ggctacatta attgatatag ataatgaaaa tggtgagcta gagctatctt
     3901 ttcgtcatat tgatgagcaa aaaagctggg cgcttctcca aaaaaattat gccgataaaa
     3961 ctaatattaa agttaaagtt ttagatgcca atcgaggtgg attaatagtt gctctctctc
     4021 aactcactgg atttctacca gtatctcaat tagcgccaaa taattatcct cgagtaagtg
     4081 gaggcgataa aactaagatt ctagaaaaac taaaaactct ggttggttct gaattagaag
     4141 taaaaatcat agccctagat gaaaatgaaa aaaaatttat tgttagcgaa aaagatgcct
     4201 ggcaagataa acaaaaagat gttatttctc gctataaagt tggtagtata gtagaagggg
     4261 ttattactgc tattactggc ttcggcctat ttattaattt tgataacgat ttagagggtt
     4321 taatccatat tagtgaactg gcttggcaaa gaattgatga tattaatgaa ctatacaaaa
     4381 taggagacaa aattagagct gagattatct ctattgacgg ctcaaaaatc ttcttatctg
     4441 ccaaaaaatt agtccatgac ccatggagag atgttgctaa aaaatacaag gtcggacaaa
     4501 aggttacggg gacggtctta aaagtcaacc ccttcggcct ttttgttgaa ctagacaaag
     4561 atatacatgg attagctcat gttagtcaat tagcccttaa acctggacaa aaaataactg
     4621 aagcctatac accaaattct aaagctgaat ttataattat ttctctagag ccagataatc
     4681 atcgccttgg gctagctagt ataaataaaa aagtctctag ggcatcatcc tctaataatc
     4741 cgcccgaaga caatgaggag gaacctaaaa aagtaaatga ccagtccaag aaagacaaga
     4801 gtcagcaaga caagcaatca ccaatagata aagagacaaa aaataacaaa acacaagacg
     4861 ctatttctaa aaagacaggt aaaaaatcta ttaaaaaagc aaaagactaa tagataaatt
     4921 gtcaatctgt agttattaga ctatactgaa ataatcatta caattttaaa ttatgaaaaa
     4981 tttatttaaa aatttttttc tagtcttaat tatttttctg acaattagtt atctatttag
     5041 tttatattct agtaaaagta cgcccgtaga aaaaattggt ctagaaagac tagtgcaaga
     5101 aattaataat gaagaaattg ctagcattac tgttaagggt gataatttat ctattgtttt
     5161 gaaagataac cgagagcaaa ctgctgataa agaaccagga gagagtttaa gccaattatt
     5221 acataactac ggggtaagtc cagaaaaaat aacggcagtt aatataaggg taaaaaatga
     5281 ctctactgct accattttcc ttaccactgt cctacctctt cttattccta ttctagtttt
     5341 tgcaggttta ttttatttta ttatgcgctc agcgcaaggg gttaacaacc gagctttaag
     5401 ttttggccaa actactgcca aagaaataga ccgccacaaa aataaaatta catttaaaga
     5461 tgtaggcgga gtaaaagagg ccaaagaaga attacgagag gtggtacaat ttttaaagca
     5521 acctaaaaaa tttactgacc taggagctaa aattcctaaa ggtattttac tgcttggttc
     5581 accgggaact ggtaaaacct tactggctcg ggctgtggcc ggtgaagcta atgtcccatt
     5641 ttttagttta tctggatcag aatttgtcga aatgtttgtt ggagttgggg cctcacgagt
     5701 tagggatcta tttaaaaagg ctaaaaagag tgctccctgc attgttttta ttgacgagat
     5761 tgacgctgtt ggtcgtagac gcggcgccgg tcttgggggg tctcatgatg aacgtgagca
     5821 aaccctaaat caaattctag tagaaatgga tggctttgaa gctgatacca atgtgattgt
     5881 tatggctgcc actaatcgac ccgatgtcct agatccggcc cttttaagac ccgggcgatt
     5941 tgaccgccaa gttattctag atgaaccaga tattaaagat cgctatgaaa tcctcaagat
     6001 acatgccact aataaaccac tagcccaaga tgctgacctc cacttagtag ctcaacgcac
     6061 tcctggtttt agtggggctg acttagctaa tgtgctcaat gaagcagcta tcttagccgc
     6121 taaaaagaat aaaaaagtta ttgataatga agatttatta gtaagtattg aaaaggttat
     6181 gctgggtcca gaaagaaaaa gccgtctctt aagtgataaa gaaaaagaaa tcaccgctta
     6241 ccatgaagca ggccatgccc tggtagctca ttttttgcca catactgacc cggtacaaaa
     6301 aatatcaata gtcgcccggg gacaagctgg tggctatact ttaaaaatgc cagaacgcga
     6361 taagcatctc caaagtaagc aagaattcat tgaagaaata gctgtgcttt tggggggcta
     6421 tttgagtgaa aaaattgttt atggcgaagt aactaccggt gcaacctctg acctcagaca
     6481 agctactggc ctagccagaa gattagttac tgactttggt atgagtgaaa aattaggccc
     6541 acgaactttt ggcgaaaaag aagaaatgat ttttctgggt cgggaaattc acgagcaacg
     6601 cgattatagt gaaaaagtag ccgaacaaat tgaccaagaa attaataatt ttatccatca
     6661 agcccgcaca atggctgaag agataattaa taaccatcgt caagaattag agaccatcag
     6721 tcatgaactc atcaccaaag aaacgattga gaaagatgca tttgaagcaa tggttgggcc
     6781 taaaccgctt gcttaaataa attacctctt tctttataat tcttaaataa gccaaaacta
     6841 gcacaagcag gcgataataa tacaatatcg cctgcttgtg catatttttt agctaaattc
     6901 atggcctcag tcatactact ggctgacaat atttgctgtg gcggataacc agctgacaac
     6961 aaactatcct taaaacgagg tgaagcctct ccctcaaata aaatgaccac tttaacttgc
     7021 tggctaataa tcaccttagc taaagaagta aaatcattgc ccttgtcagc tccaccagct
     7081 aacaaaataa ttggctctct aaaagattta atagctatta tggtagaatc tggattagta
     7141 gcaaagctat catcataata acgaactcca tttttctccc ccacaaactc caaacgatgt
     7201 tctagtccct tgaaattagc taccgcctcc ttagcaattt ttaatggcac attaactatt
     7261 tttgtcgctg cccaagcagc agcaacattt tcttgattat gttcccctat caatttacta
     7321 ggccaagtca gtcgtccaaa ccacttaatt ttccctggac aaagcttctt gtctaataaa
     7381 ttagataatt ttttattaat gactaaccaa tcgctagaag tttgatgtaa aaaaatatgt
     7441 tctttcgcta gccaataatc atcaaaactc ttatgataat taggattaac tggatcagcg
     7501 ggcgctaaat gttctttata aagattggta ataacagcaa tatggggact aaatcttaaa
     7561 tcctctagct gaaaactaga caactccaaa accacccatg aatccttatt tagttgatca
     7621 aaaaaggcaa atggcggtac accaatatta ccccctaaaa agactggcca accagctaac
     7681 tttaaaatgt cataaattaa gctagcagtt gtccccttcc ccttgctacc ggtaactcca
     7741 atgatgttct tagtaggaca aaactcaaaa aataaattca ttggactgtt taaataagaa
     7801 ccatgccgta tagcttcttg aacctgggga caaaaaatgg gccagcccgg tgaacgccaa
     7861 agttcatcat aatcattcaa tgacctatat gatttacggc ctaattgcca ctttatggat
     7921 aaattactag ctaaatgatt aattttttct gatttatctc ctaataaagt tgcttcacgc
     7981 cggtcataaa tagataagtc taatggtaag ctctgcctag ctaaccatag gatgagtgct
     8041 tcattttctg ttcctagccc taaaactgct atttttttat tggaataatc cttcaaatta
     8101 atcatactta taattcagcc caactattac ctaccttagt actaactact aatggtaccg
     8161 gtaaggcaat tatttctgtc attaagggtg gcacagcagc agtaatttta tctactaact
     8221 cctcagaggc ttcaattaaa atttcatcgt gaatttgcaa tactattcta gcctgaaccc
     8281 aggcgaaatt atcttctagc caatgatcta cattaatcat ggctgctttc ataatatcag
     8341 cagcagtacc ttgaactgga aaattaatag ccattctctc agcagctcgc cttagggttg
     8401 gattaggact taataggtct ggcaaatatc gccgacggcc ccaaaaggta gtaacaaatc
     8461 catcctgacg tgcttttatt aataattcat taatatactt atgaatctga ggataaatag
     8521 taaaatattg ttcaataaaa tctttagctg ctccataact aagaccagtg gctctagaca
     8581 gcccatgtgg tccttgacca tataaaatac caaaattaac agctttagcg gctcgacgtt
     8641 gtttttcggt aacatcttct aatggaatcc cattaatttt agccgctgtc acggcatgga
     8701 tgtcatgacc cgccaagaag ttagcaatca tgctatcttc gtgggctaag ctagctatta
     8761 ttcttaactc tatttgagaa taatcaaaac taattagttt atagccttgc tcgggcacaa
     8821 aagcttgacg taattttctt cccatctcag tacggatagg aatattctgc agattaggat
     8881 cactcgaaga tagacggcca gtagcagtta tggcttgatg aaaattagcc cttagtcgtc
     8941 catctttagt actaataagc ttaggcaaag ccaaagcata agtatttaat aacttactaa
     9001 tttctcgata ctctaaaagt aaagaaacta tggggtgctg gtctttaatc ttttgtaaat
     9061 tatcggcatc agtagaggga ccattttttg ttttatctag cccctttgaa ttaagtgcca
     9121 acttcttaaa taaaatatcc tgaagttgct taggagaatt aatattaaac tcttcaccag
     9181 cctgattata aatttcttga gataaacgat ttaagctgtc ttgccatttt tgagctagat
     9241 tggttaacac ctcttgatta attttaatac cagctagctc cattttagct aaaacagtaa
     9301 taagcggaat ctcaatttca ttatatactt ttaataagta agtatcagtt aattctttta
     9361 tcaaggcctc atggatatcc ataatttgta aaaccccaaa agcagcatct ttggggctac
     9421 cgcttttttc tccccactca aaaccgacct gccgaccaat atccataagg ctaatctgtc
     9481 gctctccggg atgtaaaaga taattagcta gtaggggatc accgcttagg ccttctaaaa
     9541 caatttcctg agttaataaa aatttaataa cttctttggc ttgaaagata atttttttag
     9601 tatttttatc ctctaaaatt gttctccatg gttctaaaat tggattccta gatgtggaag
     9661 gggtaaatag ctcaccttga atttctttac ctataactaa ctgataaatc tcattctgac
     9721 cggccagcat taaactagtt atccgcttgc catcaaatgc tggacgaaga gctaaggttt
     9781 ttccggtcca agaatgagct aggttggttg attcattgac attattaatt tctattatcc
     9841 ttgattgact aattgcgaaa actgccggcc tacttttaga atagtgggat aatttttcta
     9901 gtctaggaat aagactgtta agttgccatt ttaaaaaggc ttgacgagct aaaataaaat
     9961 caaaaccagt aaatttagct tctttcaatg aaaatttaat tggtacatta gaattaatac
    10021 tggccaactt ttggcttaag tctaggcttt cttgattagc cagaataagt tgacgccaac
    10081 gtggttttat cttaatgttg tcgccagcag ctacagcggt ttgaatatta tctaggctac
    10141 caaatgactg tagaaggcta accgctgtct tgtcaccaat accaggtact cccataatat
    10201 tatcactagc atctccacgt aaagatttat aatctactac ctgttctggc tcaaggccca
    10261 aaattctttt tactccttcc ctgtctaaaa tgaccgtctt ttgcaaacct cgctgtaacc
    10321 aataaacact aacatgatcg ttaactagtt gaaaggcatc tttatcacca gtgacaatat
    10381 aattatgaat acttttaggc gtatgactca caattgttcc aataatatca tcagcctcat
    10441 aaccagctaa ttcaataata ggaacattga tggccgtaag cagctcttta attattggaa
    10501 tctgactata taactcctcg ggctgcttga ttcttttagc tttataatca acagataact
    10561 catgacgaaa agttggctca ggtctatcaa aagctaccac taaataatct ggcgataact
    10621 cattaataac cttaaataaa acgctaaaaa aaccatagac agcattagtc ggctgattat
    10681 tagaagaaag ccccgactga atagcataaa atgacctata ggtaacggca ttcccatcaa
    10741 caactactaa ttttttatcc gcactcggca tattaatatt ataattgatt tctcttattt
    10801 taagcaataa aatatacccg ctttaaagcg ggtatattaa tagctatctt aaagattcac
    10861 ttatggccga cttcatatca tcgctccatt tatcttgcca atcacttggc tggtggtccc
    10921 aaaattggat tacatacacc cgaccatttt tagtcatctt tttttcaaaa gcctgtttcc
    10981 cctcatcatc atcttctaat tgattccaag cagcttcaga ataaactcta ataactattg
    11041 gcttggcata actaggaaca acctcgtaaa catagtttgg atcactagtt ggtacaaaaa
    11101 atctaatttc agtataatca gccgcattaa atctttctac ttgataatat ataaattctg
    11161 gcggtagatc caaactaaat cccagctcct catttctata atgatataaa ttatctcgcc
    11221 ctaactcatc catattgggt actgatctat ttttcataac ccaaattaat attccaccaa
    11281 taataattat tgcaattact aaccaaatcc atcgtgggaa cttcttactt ttgggcataa
    11341 gctaattatt taatctttgt atctttaatc tgccacacat ctttgatgtc ttgctgaatt
    11401 ttccacaatc tataagctgt ggataaaaag gcataagcca atagcagaat taccatcccc
    11461 ataactaatt tggccataaa ggtagtccaa actgttaaaa aactaagaat caatagcaga
    11521 atgccaacca cagttaaact aataactatc tgcctaattt ttttgttaac aattttaata
    11581 atgttgtcca tatttttata gcttaaatta attaacttat aaaaagtata acacaataaa
    11641 taaagattag ctatgatact attacttgtc cccctccctc caccagtgaa ccattaacgc
    11701 aaaaaccagc agcctgacga tgcccaccgc ctcctagaaa atttgccagc tcggacatat
    11761 caatattagt taaatgagac ctcaaacttc ctttaatttg accattctca gtttctatta
    11821 ataataaact accattaact ccttggagac cactcataag accagtaaga ccagtaagat
    11881 catcaacgcc taaaccaaac tcagatagtt ctttttgttt tactgtagct atggctacta
    11941 aatattcata attaatttta agacgactta aaactaaacc ccataaatgc ataactgcta
    12001 aatttttatt agttacagtc tgccttacaa ttttaggcaa ccttgcccct attaacatca
    12061 ttttagccgc tattttagca attttatcgt tagtattaga atataaaaaa ttaccagtgt
    12121 cggttaatag gccagtcaga attgatttgg ccatttccgc attaataggc cagtcattag
    12181 ctaacagaaa attataaatt atctctactg tagcagccgc cccactttcc ttaaaactta
    12241 tatcggcata atcttcttgt cgctcgtggt ggtcaaattc aataactatt tgattagcag
    12301 agcgactaaa taaccactct tttaatttac tacggctagc tgtggcacaa tctactgtaa
    12361 taatgaggtc gaattcatca aattgctctt tagttatctt gtttgctaac tggttaattc
    12421 tggataaaaa cccaaacttg tcatcaggag cactagcaca ataagccatt actggcttat
    12481 tcatagcggc agctagctct aaaaatgtat taactgaccc cagggcatca ccgtctggat
    12541 taaaatgagt agctataagc caatttttgg cttcacgcat taacgaccag gccttaagag
    12601 cttccacttt atccatataa aatcagcttg ataatttatt gattaaataa ttaagatggt
    12661 ccgcctcttc agccgaatca tcaattaacc aaataattct aggtattcgt ggcaattgat
    12721 attttttagc taaaaattta gcaatttctg ccgacttctt tcttaatttc cttaaggctg
    12781 taccacgcaa attaattgga agtacagata ctccaacttt catttctaaa caatcagcat
    12841 ttaaactggt ataagtaata gataataaac ctccgggaaa ttctattaat ttattaacta
    12901 aatcggctat ctctcgttgc agggttttat tggcgcgttt aaaattatga atcataaatt
    12961 acatcttttt aaaaacctca tcttcacgat aaatccgtaa aatatctcct actttaagca
    13021 cttccaagct ttctatctga agaccacatt cttggtcagt aacaacctct ttaacctctt
    13081 gccgcccggc ttgcaaatgg actaatttac cttgaccaat aacctcccca ccccggacaa
    13141 cttccaatag actatcggcc cgaatagtgc cctctatgac tttgcctcct actatttgag
    13201 tcattccttc ttgtttaaaa attgccaaca ctttaacctg ccctaaattt attcgtttaa
    13261 tctctggctt cattagggcc tgcatatctt gtctaacctt atctatcaaa tcataaataa
    13321 tttgatatct ttcaataagt acccccttgt ctctagctaa atcttcaact tgggcattta
    13381 ctccaacatt aaaaccaata accttagcct gattagctgc agccctgaca acatctcctt
    13441 cggtaatatt tcctagccca cgataaataa ttcgaacggc tacctcttca ttatttaact
    13501 ttaaaagaga ctcctcaatg gcctcgccac taccataaac atcagttttt atcactaaat
    13561 taattggggt ccctttaaaa gaattatcat cctgcacttt agtaatgatg gcttgatttt
    13621 cttgtttggc aattattttg gttttatttc tatgttctac agattctacc cttactatat
    13681 caccaatttc tggtgttgcc ctaaggccaa taacctttac tggagtagat ggaccagctt
    13741 cttggattaa ctgaccacga taatctttta aagcacgtac cttaccataa tattgttgat
    13801 tgcacatcag attatcgcca atctttaaag tcccattatg gactaaaacg gtagctactg
    13861 gcccctcccc tttatctata tgagactcaa taatagtccc cactgcctta gctagaggat
    13921 cagctaatcg agcctcggcg ttcaaatcag ctaacaataa aatagtatcc aaaagctcat
    13981 ctattccctg gccatatcta gctgaaactg aacaaataat tgccttacca ccccactcct
    14041 cgggtacaag gccaagttca ttagctagct cctgcttcac tctgtcaaca ttagcctctg
    14101 gtttatcaat tttgtttata gcgactacaa aaggaatctt agcctcttta ataattcgat
    14161 aagcttcaat agtctgcggc ttaattccat catcagctgc tattactaaa atagctaaat
    14221 cagccacttt agctcctcgt ttacgcatag cggtaaaagc ttcatggcct ggagtatcta
    14281 caaaagtaac ctccttatct ttacgactaa tctgatacgc tccaatatgt tgagtaatac
    14341 ctccagcctc agttgctact acatcagtat gacgaatagc atctaataat tgagtcttcc
    14401 catgatcaac atgacccatt accacaacaa ttggtggcct aactattaaa ttattagaac
    14461 cagatatatt ttctgcttct tttaattcat taatatcatt atccatcgct tcttcttcag
    14521 aagccactac tcctattcct aattcactac caataatagt tgctgtatca aaatctattt
    14581 tctcgttaag tgaggcaaat attccattct gcattaattc agtcaaaata cgatttactg
    14641 gcaaaccaaa aacattggcc aggtctttaa cggtgatgac tggacctatt ttaactactc
    14701 gcctttcagc tcgagctgtg gctactgctt cttcttgttg tcgctctcgc tcctcacgat
    14761 tttttaagtc acgagtcagt cgtggccatt ccctaataat acgttgagcc aaatattgat
    14821 ctatcttaat agccttttga ccaatatcaa aaccaatctt aggcaacgta tctcttaact
    14881 cttgaggtga aaccctaagg cgacgtgcta attctgtaat attcataaaa atgattataa
    14941 tcctttagga aaaatttatt ttgtccttac tttataatat ttttccttag aagtcaacgt
    15001 ttatatctat aaaaacccac attttagtgg gtttttatag atataaattt taattatact
    15061 tactggcgat gaaaagcgtg actaactctt ctgataatat ttttgttact tttattgggc
    15121 aatttgtcta taaaactaag ccataaacta tattcaaaca cagtcactat gcccacaaca
    15181 aacattaaac cggctattaa taaaatctgg gcaataataa taagtggcac ttcgctaaat
    15241 gataggtatc ttattaagag taaagacaaa agtgaataag gtatagctaa tagacccata
    15301 ataaaaatcc acaacaaata aactactaaa ttgattaaaa ctacaataat aaaaatccct
    15361 aagctggcca ataaattttc acagagtata tgccaagcct ccttggctga agccaaccaa
    15421 tcacttttct tgtcagacaa aaagagtaga agccaataaa agccccaggt acctattaac
    15481 caaattaaaa ccaggaatag taaaactaat acaatataaa caaaaacgct gataattggc
    15541 tgtctaatta aataagctcc tgtcatgcct aaacttatta tcaataatcc aaataataga
    15601 attaatttaa cagtaacaat aatacctact gtcggccaaa aaattggcca attatttttg
    15661 atctgttgcc aaaaacttac ccggggaaga ggtagggact tcttcccttc agattttata
    15721 ctatgctgta ttttctggac taatacaatc tgactattaa cagcaagaaa caataaacca
    15781 ataattaata ctaaagctaa taaacacagt aataaaagta aaggattatg agaggaaata
    15841 ttaatcaaag ataaaccata attccaataa acagaaagag agttcaaaaa tctagaccag
    15901 ccacttccat caaaagcatt taataaactg gtatattgat tgacattact aagcaaagca
    15961 gcaaataaac ccattaccca taaccatcga tttttaatgg ttatctgcca agtttgcttc
    16021 agtatatttt tataattgat catagaaata tttaatttga cttaggagtc tttttaaacc
    16081 aataaagctg ctgaaaaatt gtaaagagac tagaaaacaa ccaatacatt gctaaaccac
    16141 tcggtagagt ccaacagatt ataactgtca taatgggcat cataaatatc atctgcttgc
    16201 tcatcattgc cgtaagatta tctgcccggc cattaccagt attagcaggc atagtataat
    16261 gagccgaaat tttactctga aaaaattgaa ctaatcctgt agctactgcc aaaatttcat
    16321 ttggtttagc taaatctatt ataccaaaaa atgtagtttt taacaattct ggtctactaa
    16381 taaatggata gaccaatgat agcgactggt tagaaagacc atcacgaaaa accataaaca
    16441 ccgcccataa tagtggcaac tgaattaaaa ggggcaaaca agaagaggcg gggttaacct
    16501 tttcttgacg ataaagatcc attagggcct gaccctgttt ttccttgtca tttttatatt
    16561 tattttgaat ttctttaatt tttggctgaa gacgattaat ggcctgttgc gactttaacg
    16621 acttctgaga aagtggccac aaaattaact taaccaggat tgttagagca ataataacta
    16681 ctcccatgtc atgaccaggc aaaatattat aaaaccaaac cagaagattg agtactggct
    16741 gataaaaaag aatagtaaat aaatgcttca taaaaattag cgaactggat cccaaccacc
    16801 cgaggaccag ggatgacaat gtaagatgcg attaatagat attagacttc ccttgattaa
    16861 gccgtatttt tttagggccg cctgggcata ttctgaacag gttggccgaa aacgacaaca
    16921 gccataagga aaatactttt taaaccaacc atgatctggt gacaaggttt tttgataccc
    16981 tgaaataata gcaattatta aattgattac aatttgctct aagaacttaa taacccttat
    17041 catactatta attttagttt tttccaagca ttaattaact cttgtcttaa gtcaataaac
    17101 tttaaaaggg gccagggagc aataacaaca atcacaatat cgtggcctcc aaccatcttg
    17161 gataaatttt cttctactat ggttgctaaa cgacgcctaa ttaaattacg ttctacggct
    17221 aaatgacagt aacgacgact aacgacaaag ccaaagcgat tataatctaa tccattagcc
    17281 actattctaa cacccaacaa tttaccataa tatttagcct cggctgatct aaaaagacgg
    17341 tcaaaatctt tcttcttggt taaacgattt tgttttttaa gcatagaaca agattaacca
    17401 agaactattt ggttaatctc tggcgcccct tattacggcg gcgacgtaaa acactacgac
    17461 cacttttagt catcattctt tctaaaaaac catgcttctt cttagctcgt aaattattag
    17521 gttgataagt tcgtttcggc atattatcaa aagttaaaaa gactccttta gtgatatagt
    17581 ctagcaagag catatcagtt tattaaacct tggtcaatac ttataatgat tttattatcc
    17641 acattttatc aacaaagtta tcaacttttt aatgggttgc cgaaaaaaat aagcctgtta
    17701 taataaaatc aaaataagcc cccatctatg gatcactcct ccctagaaca aatttggcaa
    17761 gctatcctta gtgaagctga actgaacatg tctaaagcca gttttaccac ctggtttaag
    17821 aattctttta tttttgatta tcaagatgac attttaacta ttggtgttcc taatgccttc
    17881 actcaggctt ggtttgaaaa taaattttcc aaagatatta ctaaaacttt ttataaagtt
    17941 accggtaata agcttaaagg gataaaatat attatcaaaa ctaagcccaa ggaagctaaa
    18001 agcaattttc ttgctcctga ccctcctgaa gaaaaagaga ataaggccta taaaactaac
    18061 ccctcaaaag atgaatcact ttgcctggca gctggtctta atcctaaata tacttttgaa
    18121 acctttatcg taggtaaaca taatgaatta gctcatgccg cttcatgggc agctatccgc
    18181 caacccggag aaacatataa ccctcttttt atctatggtg gcgtcggttt aggtaaaact
    18241 caccttctcc aagctattgg tcatgaagta ctcaaatctg gtaaaaaagt cctatatgtt
    18301 acctcagaaa aatttactaa tgattatgtt gatgccgtct atcatggaca gggtaatact
    18361 tttaagagtg tctacagaaa tgtagactta ttactagttg acgacataca atttatggct
    18421 ggcaaagatg gtacccaaca agaatttttt tatacttttg atcagctcca ccgagccaat
    18481 aaacaaatag ttattacttc tgaccgttca ccgagaaata tgaaagctgt agaggctcgt
    18541 cttattacgc gttttgaatg gggtatggtt gctgatattg ggcaacctga tttagaaacc
    18601 agaatggcta tcttaaattc taaatgccaa cagaaaaact ttcccttaga ccaatcaatt
    18661 attcaataca ttgccacgaa tattagtaat aatattcgtg aactagaggg agcacttaat
    18721 agaattattg gtttctgcca attccataat tgtgaaccag atttatctat cgccaagcaa
    18781 gtactaaaag atttttttat ttccattcaa gctaataatc tatctactcc ccaaaaaata
    18841 ttagaagcgg tggctaattt ttataaccta aatttatctg aacttaccgg cagtagccgt
    18901 aaaaaagaat tggttatccc cagacaaata gctatgtatt tattaagaac cagaatggat
    18961 gcttctttcc cttttattgg tcaagaatta ggtggacgag accatactac tgcgatgcat
    19021 gcttgtgcga aaattgagcg agatattcaa aataattcta aaattagaca agatattgaa
    19081 caaatatctc aatcaattac ttcggtgtat taattgtgat taaatagttt ataactttta
    19141 tttatccact gactaaaaat ttttaattaa tttatatact tgttattaac aataatttct
    19201 ctctttatcc acaattcaaa tactaatatt tttaactaat attatacgta aaaaagactt
    19261 atcaactttt ttccagctat tattaataat ataatctata ttaaaataat tattatgcaa
    19321 ttaactattc ttcaaaaaaa tttacatcaa ggattaacca ttgttagcca tattgccgaa
    19381 aaaaatctta gtatgcctat tttaaataat attttaatta aagcaaaaga taatattatt
    19441 actttagcag ctactaactt agaaataggc atcactaagc aaattagagg taaagttaat
    19501 aaagaaggag gaattgctat agatgctaaa atttttagtg attatattgc tcttttgcct
    19561 aacgaaaaaa ttgatattca attaataaat aaacaagatt taaaaattaa ttgtaaaaaa
    19621 tacaatacta ttataaaagg acaaaatgct gaagattttc cagtaattcc tcaaattgat
    19681 aaaaaaatca gtctcacttt agctgtttct gatttaaagt tggcccttaa tcaactttta
    19741 tttgccatta atcctactga taatcggcta gaattaactg gggttttatt cgatattaat
    19801 aataaatata tcaatctagt atctacagat agtttccgtt tagctaaaaa agaaattaat
    19861 tttcatctag attttggcac tctagattca gaaggactta aaattattgt tccttttaag
    19921 accatgcagg aattagcaag aattattaat ttttccaatg atgaattaac tgaagatagt
    19981 gatcaaatac agatacatat cgctgacaat caagtcttat ttgtttatca aggaatagag
    20041 atagtgtccc gtttaattga aggacattat cctgattata ttcaagttat tcctactgat
    20101 tttaaaactg aaataactat tgaccgagga gaatttttaa gagccataaa aatggcctcc
    20161 atttttagcg ccaattctaa tgccgttgat ttgactatta atgctgatca gcaagaaata
    20221 ataattgaag ctgtgtcagg acaaattgga agtcatcagg caaaattagc tgcccaaata
    20281 aatggtaaag ataactctat ccgcttaaat tatcgctatt tattagattg ccttaatatc
    20341 cttccaacta atcaggtttt attcaaaata attgatggta ttagtgcatg tgaaataacc
    20401 ctacccggta gtaatgaata tcaatatatt gtagtccccc ttcgtaaata attatttagc
    20461 gtgagctata gtaagaagag taaaaagaga tgcctcattt ccatggcttt gtttagttga
    20521 ccaattaaat tttgttaaat tgtctaataa ttgaattaaa ctgtctcttt ttagttttga
    20581 ggcttgatta attaattttt gactcacact tttgggtagg ttaagtgatt tttcaatttc
    20641 tattaaagat tgtcctgcct ctaggcgggt tttgatgtct aaaaattgga gtacttgtcg
    20701 attaagtagg gctaagaaag tggcaatact gtcttcatcg ttaaaaatct cttcaattaa
    20761 tttggttgtc tgggctaatt tttggttaca aaaggcctct ataaattcaa ataaatgttt
    20821 ggtggtgtta tttactaaaa gattaacatc atcagcatca acgacttttt tgtggtcact
    20881 accagcaata tagttggcta atttattaag ctcgtgataa atctgccaga aattaccacc
    20941 agttagttct aataataatt ttttagcagc tggagtaagc tctatatttc tttctttaat
    21001 aagctgctct aagaattttt ctgcacccag tccttttagt tttggataat attttactaa
    21061 tttagccttc tgagtaagaa gtaaaaataa ttcttgctca tttttattaa gtggtttcgt
    21121 tcgcccatca cctcctagga ttaccggccg ttgttttccc gccagatatt tcattcctag
    21181 attatagtca ttaataaaca aatagataga tggcgactca ataattgttg tttctttttt
    21241 taattcatta attaatccat tttctactat tgaatttttg ccactcaata aattattaag
    21301 agtaataatc ttagtagact gaaaaagtga tggattgtta agactgttat taagttctaa
    21361 ccaatctgtt tcaggtatat taaaagactg caggctggca acagtattag aatctaattc
    21421 tcttagttgc tgtttataat cccgctgaat ctgaaaactt tcttcaccat agcggaagaa
    21481 aagcataaat tttgtttatt tcttagcagt ttttttggtt ttagaggcag aagtactggt
    21541 ttttttagtc acttttttag ctttttcctt tgaaatgact ttagttttat tagtcttttc
    21601 tttcttgcta gtttctttgg tcggagtttt gtttgtttta tccgctgaac tgacgatatc
    21661 taaatgccag ccagttaact tagcggctag gcggacattt tggccatctc ggccaatggc
    21721 taatgataat tgttctggat caacagtaat tgttgcttgt ttattctcat agtcaagctc
    21781 tacttgttca atttttgctg gggctaaagc attagcaatg aatttactgg gatcatcact
    21841 gtattcaata atgtcaattt tttcaccacc aacttcatta attatagttt ggattcgcga
    21901 acctcgttga ccaacacaag aaccaatagg gtcaacattc tcactgttag cggctactgc
    21961 taccttagac ctactgccag cttctcgggc gactgccttt aaatctacta gaccgttaga
    22021 tatttctgga atttcgagat aaaaaacctt actaagaatt tcgtcagctg ttctagacaa
    22081 aataatttct ggccctcgat tgcttactcg aacttcttct acaaagactt taatgcggct
    22141 attagggcgg taattttcgc taggtatttg gtactggggt ggaataatgc caacagtttt
    22201 ccctacatct actaaaacat tatggccttc acggcgctgg acaattccac taattacttc
    22261 tttttcttta tctttaaaat cttcataaat catatcgcgc tcagcttctc gtaatttctg
    22321 gatgattact tgtttagcag tctgggcagc catacggcca tattcagtat gagctggtag
    22381 ctcggtaatg atttcatcgc ccactttagc attcggttta attagtctag cttcgctaag
    22441 ttgaatttct gttttaggat taaattttct aataatttca ccattttcgt cacggatttc
    22501 tgtcggcggt tgatcttcac gaatggtggt taattcttta gctgcttctg gatctatagt
    22561 tactgttgac tcttgtaagc tctttaaggt tagcggagcg ttaaaggttg ggagaggcat
    22621 atcttctact accgttttaa catcatagac tttgatctta gataaatcct cattaaattt
    22681 aactttaatg ttttgatttt tgtcgccata gtctttacga taagcggcag ctagggctga
    22741 ttcaa
//
LOCUS       DAOOIS010000007        22388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_2353_length_22388_cov_39.157704, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000007 DAOOIS010000000
VERSION     DAOOIS010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22388
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2353_length_22388_cov_39.157704"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PK960_01460"
     CDS             <1..846
                     /locus_tag="PK960_01460"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HPD07872.1"
                     /translation="LIGRGYNYHWLPSGVGLASFLAAFAYSGAGGNLNLSQSFYIKDK
                     GYGMGLYAQKISGLFRSNQEQEVNLEGPDFTPNPTNLQRFRQWWKKISLEHLLVFWFL
                     GFLAMALLMILSYSTAFGLNNNQEGINFIFNEAKLIGQTLAPWVGSIFLILLGIMLAQ
                     TQLGVLDSTSRIMAENAALLKLQNKKEKKLNLSKIYYAFLWGQIMFGIILLLLNFYEP
                     KQLITIGAIINAIAMFVHIGFVNIGNWKLLPPSTRPGWPRRIIMLIIFLLFAGFGGIT
                     LFSYL"
     gene            complement(850..2082)
                     /locus_tag="PK960_01465"
     CDS             complement(850..2082)
                     /locus_tag="PK960_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD07873.1"
                     /translation="MKFHAALQLSIRSLRLNLLRTTLTVLGVVIGVAAIVVVFAAGDA
                     VRNLVTEEISSYGTDIIETEIRLPKKRTASALAQVTTLKVEDMEAVNKLANVKQSYGA
                     ILGQQKVSWQGQSERSLLFGVSAPYVEIDQKSHPIEGRFFTAEEDRNQAMVAVIGYSL
                     KEELFGDQSPLGKWIKIGHNKFQVIGVMQKRGRGGNSQIDIDKSVYLPLKTLQKRILG
                     VDYILYMVHQLKDPTKADDTAEEITSLLRERHGINDPDKDDFRVITMAEMMKTLNTVT
                     NTVTWLLLAIVIISLIVGGIGIMNIMYVTVSERTPEIGLRKALGANYHDIVEQFLMEA
                     VLITIWGSVIGIIIGLFLAWILSIVARHFSIEWSFIFPWSGIGVAIIFSLACGLLFGF
                     RPAQKAAKLDPVEALRAE"
     gene            complement(2089..3333)
                     /locus_tag="PK960_01470"
     CDS             complement(2089..3333)
                     /locus_tag="PK960_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD07874.1"
                     /translation="MGEELKQSLSLAWRALMSNKVRSFLTMLGIVIGVAAVIIIMSIG
                     NGAQSLILDQLKSFGKDLVIVLPGDSDGGPPSETLGITVTTLKYDDAKALSEPNNVAN
                     VKAVAAYYQTSANVIWRDKSYETIINGVTANYFDVEDSSIEQGRFFTNEEVDSAAHVA
                     VLGSVVVDELFGDVDPIGQRLRIKNQTVEVIGVLKSRGKVGMQDYDDQILVPVVFAQK
                     MIAGVDYLSSIRVKLVPNADADRAVLEIEETLREQHNILKKEDDDFSVHSFQDVLDTI
                     TRITNTINYFLATMAGLSLVVGGIGIMNIMLVSVTERTREIGLRKAIGATNGQILRQF
                     LLESVCLTLIGGSIGIISGVLVSYLAAVVAHLLRYQWDFVISIPALLIAVLISIAIGI
                     IFGLYPARRASNLEPVDALRYE"
     gene            complement(3338..4063)
                     /locus_tag="PK960_01475"
     CDS             complement(3338..4063)
                     /locus_tag="PK960_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD07875.1"
                     /translation="MADYPLIDVRHLNKIYKNDGVVTQALFDITFQIKHGEFVSIMGP
                     SGSGKSTLMHILGLLDRPSSGEYFFNGHNVNELSDDELANLRNKSIGFVFQAFNLLPR
                     TTVLDNVKLPLLYSKDKRRMEERAKEVLENIGLGHRLYYLSNQISGGEKQRVAIARAL
                     VNNPQVIFADEPTGNLDSKSGIQVMQILQALNDKGTTIILVTHETFTAEHAQRIIKIY
                     DGRLVSDEPVVNRRIAKENEFLK"
     gene            complement(4065..6038)
                     /locus_tag="PK960_01480"
     CDS             complement(4065..6038)
                     /locus_tag="PK960_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPD07876.1"
                     /translation="MQKHKKTWLIIIGIVLVVGLILGWAAWRGSKQNKPQYTTVQAEA
                     GPLKQTVSVVGTVKPLKELALNFLTSGRVSNVAVKVGDSVKAGQLLGELDYSALLLRK
                     NEAEAGLQIAQANLNKLLSGASQQTIAVSQASVEQAVANQKMAQAELDKLNKTVAENI
                     KQAEKTLNDLTDPSPETATVQEQAVATAQTNLTNIDKTTQENLTNAYNSGLIVFSDKI
                     LAAKVALDHINTILTDDDAKYVLSVKDSVYLQRTKDTRTLALESYTAASQAVTAARDG
                     FSDNAFTIASDKTKQFLNQTAQALSYAYSMLEATITSNNFSQAKLDNYKSIISGQINQ
                     INAAITAAESATQSYKNAKLNRETSLAAAQDNLNQAKVNLDNARQAAQNNLNTVRLTG
                     ERQIANAQSQLDAATKAVEVAKAQLENVKAPARAADITLAQGQINQALANLASVQKQI
                     DDSRLVAPLDGVVTQVNYEVGEQFSAAGKPFVTILVNNNFYIEADITEADITKIKIGN
                     PVEITLDAFGPDVIFHGQVYFIDPAQTVIQDVVYYKVKISFSDSQEITRLTTEYHTDI
                     KAGMTANVTIITAERDNVVKVPARAIIDKDDGTKIVRLLKDNQLQEVPVTVGLRGDEG
                     LVEVTSGVNPGDQVVTFIKYPKKSNGLELNLNN"
     gene            complement(6152..6628)
                     /locus_tag="PK960_01485"
     CDS             complement(6152..6628)
                     /locus_tag="PK960_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07877.1"
                     /translation="MTIHHLIPRSRGGDSSKNNLKKLPWKIHGAWHTLFLNWLPEEIF
                     SQVVIMPEALYDSINKQLAWKMVFHYSWEEVMWSEAVRMDIFRQIIGGWVPTTYIISW
                     EQVNQGIWDICCAQCPMSCCPLNHHRRQPFESGSVLYYCYWPVHKDLLDFITFPRC"
     gene            6885..7550
                     /locus_tag="PK960_01490"
     CDS             6885..7550
                     /locus_tag="PK960_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPD07878.1"
                     /translation="MDLTYQLVKNDQQINSFIKTTEKYLEHLGFTDHGFRHLTIVADR
                     SKKLARRIGLTPKEQELAAVAGYCHDLGNFMGREMHHYWSALIFFQIMQPRIKQTADL
                     ATIMQAIVNHDSNHLQTDNKIAAVLILADKSDVHRSRVRQKDLSKIKEDIHDRVNYAV
                     TDNDLLVNKKAKEIILRLTIDTKEVEPINYFQIFIDRMTQCQQAAEVLGYKFILIINN
                     FRF"
     gene            complement(7547..7990)
                     /locus_tag="PK960_01495"
     CDS             complement(7547..7990)
                     /locus_tag="PK960_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07879.1"
                     /translation="MEQDKYIIDYSHNDRQKISELECVKRWLPIYNKVNKASYNCLRP
                     NYFQNEVDVYVYQQGETGRPLRLQLTKTNFQALTKTIKRKAKLYTPEQKADLLLLLDF
                     TGNESAGKIMAWQKKHLNLLVSSGFMEIWLVGFNGLVINLWPGLG"
     gene            complement(7993..8463)
                     /locus_tag="PK960_01500"
     CDS             complement(7993..8463)
                     /locus_tag="PK960_01500"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPD07880.1"
                     /translation="MLQIIIVTIGCLRSAACRELADDYYQRLGQLAKIRVIELAGGRF
                     APGQALVARHNDSIKIAQFLEHYPGAQVYLLTELGKELDSMGWASMLRRWDQDGRLII
                     LVMGGAPGLDNNILKIKETLSLSLLTWPHELARVLLLEQLYRGLTINNHKDYHY"
     gene            complement(8470..8880)
                     /locus_tag="PK960_01505"
     CDS             complement(8470..8880)
                     /locus_tag="PK960_01505"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPD07881.1"
                     /translation="MTQHKTRGGFTLVELLVVIFVIAIISTLSYVVLEGTRAKGRDAR
                     RLADIYMIRQALELYKDREGSYPINLPEPQQSLLGPSGRLYLSPMPSDPKTSQPYLYN
                     LLENGGYRLIFNLERDNQGYSAGENVIISPGDDK"
     gene            complement(9187..10734)
                     /locus_tag="PK960_01510"
     CDS             complement(9187..10734)
                     /locus_tag="PK960_01510"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPD07882.1"
                     /translation="MLIRKLSKYLFFPTLLIISLAITLPAAAQMTITDIAISKPHDGI
                     ATITWNTSEATKSDIYFGTSSDRLIYHVGNIEFKHSHEIDLTGLQKKTDYYYKIVATN
                     SSGQRVESFIQYFNTDEMKDSTAPEITGLRVLQTIDTAAAIYFLTNEPSRFDIYYGFT
                     SDKLDKHRSDSALRYEHLIIIDGLTSGSKYYFNILAKDEDGNIAERSGEFTTDAYHNY
                     DQIKISNLVPENANQAIILPEAVVISWDSNVLATSEIVYGLDPNHLNNRVQASKDHQL
                     SHRAELTNLNPSTVYYFKIKMSSSLNHKSFESQVYSFKTASLTAEYLRQYFKSGDILK
                     HGNDYYYLYNQQIIKLNDDSLVSRGYDKSVAKTIADKYFTFYKAGPAYWGAYHDGQAV
                     KSPKNPTVYVIDGQYKRPLANWSVFKYLNYKTSDIVIDNSGNLRNYKDGTLVKHSSEL
                     TGKCPLKNYSLAKSPNGTTVYLIANGHKMPFVNQKVFLRYGFKFSNVKTVDWGILGHI
                     PDGQPII"
     gene            complement(10747..11628)
                     /locus_tag="PK960_01515"
     CDS             complement(10747..11628)
                     /locus_tag="PK960_01515"
                     /inference="COORDINATES: protein motif:HMM:NF022114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HPD07883.1"
                     /translation="MKKTIFYLIIGLVIISGLIILFIKINTLFKAKEPHLTIESNKPL
                     DMSDFIVVDKPRAGDVIESPVTIVGQARGQWFFEGSFPIKIIDASGEQLGEGAAEAQS
                     NWMTNDFVDFRAVISFKSPATSSGFVVVHNDNPSGLPEKAKELRIPIVFSSNPPESKE
                     RTVKFFYYDSRLDQDESGNILCSPKGLVAWGYNIPFTTTPIKDTIEAFLITPLPEKAL
                     LAGLSSEFPLPGVSLESLNLDKDSGILTIKLSDKNNNTQGGACRVAILRAQLEAVAKQ
                     FPEVKEVRFEPETLFQP"
     gene            complement(11636..13030)
                     /gene="asnS"
                     /locus_tag="PK960_01520"
     CDS             complement(11636..13030)
                     /gene="asnS"
                     /locus_tag="PK960_01520"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPD07884.1"
                     /translation="MNLLDIKNLYRETDRYLNQNITVAGWVRTVRLSKDFGFIELNDG
                     TFLKNLQIVFDKGLSNFLEIEKLSLGSAIKVDGQLVKSPAPLQPFELKADHIEVIGLS
                     DNSYPLQKKKHSLEFLREIAHLRVRTTTFSSVFRVRSALSSAIHQFFQERGFSYVHTP
                     IISSSDCEGAGEMFTVTTMDLTQPIIKTPTGQVDYRHDFFGQRVGLTVSGQLEAEALI
                     FGLNRVYTFGPTFRAENSNTTRHACEFWMVEPEVAFADLADITDLAEEMVKYLVNYIL
                     QLLPEEIEFFNDSVDDTLMERLQKTLKANFIRLSYTEAIDLLLKSKEHFQYPVSWGMD
                     LQTEHERYITENIFGQPVFLTDYPKDIKAFYMRQNDDGRTVAAVDLLVPGVGEIVGGG
                     QREERLTVLEERIDELKMNKKDYGWYLDLRRFGSIKHAGFGLGFERFIMYLTGIANIR
                     DVIPFPRTPKNAKF"
     gene            complement(13041..13526)
                     /locus_tag="PK960_01525"
     CDS             complement(13041..13526)
                     /locus_tag="PK960_01525"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPD07885.1"
                     /translation="MNYDRIWQKIITPGEKIEYQFSVGNRYLLFYWLLWVLISIGSIV
                     VYRSPLVPLLLFLGATFFYAFYFKIANAYAFTDHRVLVHRGWLSTNLVSIDYDKITNV
                     TVEEPFFTKIFTGTGSLTISTAGSIDGGVQLKHISKPYQLKQILDNLMTKYRVNSSNK
                     Y"
     gene            complement(13580..14323)
                     /locus_tag="PK960_01530"
     CDS             complement(13580..14323)
                     /locus_tag="PK960_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPD07886.1"
                     /translation="MSSNKHRVALITGAGQGIGRGIAVQLAHDGYNIAIADINLNNLA
                     ETAREISALGVKVLKIEANVANSYDVQNMVQATMDEFGLLDVLVNNAGIYPFEPFMDM
                     TEEQWDKVISVNLKSLFLCSQAVARVMSNGGRIIMISSIASLLGFSGLAHYCASKGGA
                     NSLVRALALELAPRQITVNAVAPGSIMTPGTSNTDEESQKAIIMKIPLGRLGTPQDIA
                     AAVAFLASKEASYITGQILVVDGGWTIQS"
     gene            14424..16100
                     /gene="glgP"
                     /locus_tag="PK960_01535"
     CDS             14424..16100
                     /gene="glgP"
                     /locus_tag="PK960_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013182916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPD07887.1"
                     /translation="MISIDKDSTKIAYFSMEIALNNQIKSYAGGLGVLAGDTLKSAAD
                     LKIPMVGVTLLNRYGYFKQKISCEGDQIELPDTFNYRHLKKTNVVTHIKIGEDVVKIR
                     AWEYLIRGHSNYYVPVYFLDTNVAGNKEKYRRLNGQLYGVGLEYRLMQEIVLGRGGVK
                     ILSELGYHNIQKYHLNEGHTALATVELFRESLAKSSSAKIKEVRHKCVFTTHTPVKAG
                     HDIFPLSLAKDLQHDLPHLPKLFQNDELNMTKLALYFSSYVNGVARSHQEISKKLFPG
                     YCIYSITNGVHSATWTSPEFQKLFDKYIPTWRECSLSLRNVFSIPTSEIWTAHQQAKA
                     RLLNYIYKKQGIKLDLDVFTLGFARRFTGYKRSTLLFYDMNELLRIHRTAGPIQIVYA
                     GKAHQDDENGKQLIKEIIKIKEQYKKDIKIVFLENYDMNIAKLMTAGVDVWLNTPLPP
                     NEASGTSGMKAAHNGVPHFSTLDGWWIEGFVNRKTGWAIGERRTTLDPKQLNKRDAAN
                     LYNKLETRILPRYYHAPENWRETMRYTIAINASFFNSERMLQQYIQSAYL"
     gene            complement(16091..16654)
                     /locus_tag="PK960_01540"
     CDS             complement(16091..16654)
                     /locus_tag="PK960_01540"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HPD07888.1"
                     /translation="MKTLPSKNLLLVIFVICLLVVIALVIFLGHVPRQDIEQSSQVSS
                     SILYFAYGSNLSSNNMYSRCGSNITALGPAKLPNYEFTFDRRGSANIVPHTGQYVWGL
                     LWNIDSDCLRALDRYEGYPTLYGRQTVTVEWENNEVSALVYIESSKEAGGQPNYSYLK
                     DKVIPGAKEHNLPPAWINKLENYLLYK"
     gene            16764..17762
                     /locus_tag="PK960_01545"
     CDS             16764..17762
                     /locus_tag="PK960_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07889.1"
                     /translation="MLYFFRTLKGLKIKIIKIGFIGILLAVIIPLTVQAVTNNQGTLS
                     DSSLSNNQYLNNNNPLNSDAIAVRVFNNDNYLSAYDWYQYNVRANRSLQSLIIDGYEA
                     IRDDRTVYVAASNVVTCSSSKQCLQPIIAIISFNQNVSSETIDIFGQLLNNWRFNQNI
                     NNPKGSCLSKPATTSTIYCLSNKDCPSGQYCTSAKAKIVRDTKRLINLTSIHQKLKQY
                     YDTYHHYPTLEAGTYLINKTLSVWPSWQKTLALKLGSSLPIDPINQLGPCPAEYNPIT
                     CWNEQRRLFATPWPSLPPDSYTYQYEYIDSHHYRLCANFESKGYANLAGLNCPQNH"
     gene            complement(17835..18347)
                     /locus_tag="PK960_01550"
     CDS             complement(17835..18347)
                     /locus_tag="PK960_01550"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPD07890.1"
                     /translation="MLSKILAIIIAYKYLALFIITFLASLLNITPAMPILLVSGFLIR
                     SKYLIASITWIVGYCGVVLGDLTAYFLSFCYGRDVLVKIGFRRMLNSPKFFKVEKIFN
                     KHASITIFLSRFLVPGVGPVVNLMAGLSKTDYRKFIVCDLLGEAIFISLLIGIGYLLE
                     PTNGVVLLIG"
     gene            18620..19903
                     /locus_tag="PK960_01555"
     CDS             18620..19903
                     /locus_tag="PK960_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009537184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPD07891.1"
                     /translation="MTLLASPKIITLIIFILTYCGLIFLPKKRVLCAGIGAGLLIILQ
                     IINLPEIFSAIDWNVLLMILGTSGLVIFFSESKMPAFLGEKVIDKAPDFRWAVVFLAL
                     LAGGISALLENVSTLLILTPVALAMAKKLKTSPVPLLITIAISSNLQGAATLIGDPTS
                     IMLGNYANLNFLQFFFLNHKPSIFFAIELGAIMTAMAIFYLFRKEKQPITTEEHTKVT
                     NFFPTIILLLMIVSLIGVSFIKEKPALTNGFICSGFFLLAAFRELIIKHNLSPKKIIK
                     NLNWETLVLLSFIFIIIGAIVKVGLISDIASLIARAGQGKIFLLYSVIVWSSVLASAF
                     IDNIPYVATMLPVIDALTMQLGVSPYLFYFGLLSGATLGGNITPIGASTNITALNILK
                     KEGYTVSFKEFIKYSLPFTLAAVISGYLFIWFFWH"
     gene            complement(19977..20642)
                     /locus_tag="PK960_01560"
     CDS             complement(19977..20642)
                     /locus_tag="PK960_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPD07892.1"
                     /translation="MSRTSIVILIILLVAIGLYFYLGPKSKKNKNQSIAPVVEQPTSV
                     SVSNKKVVMVVASENFRDEEYFEPKTILEEQGVIVKTASNKAGVARGVGGREANVDLL
                     ITDVNPADFDAVIFVGGPGSLENLDNDNSYQLAQSTLGQGKILAAICVSPVILAKAGV
                     LKDKEATVWSSPEDRSSIQVLEDNGAKYVDQAVVVDGRIITANGPAAAQEFGKKISEL
                     LGL"
     gene            20750..21766
                     /gene="amrS"
                     /locus_tag="PK960_01565"
     CDS             20750..21766
                     /gene="amrS"
                     /locus_tag="PK960_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPD07893.1"
                     /translation="MKEAYLYEKLKNHNVRCLNCSHYCHLSPDQKGICGVRQNIDGKL
                     YSLNYGQVVALNIDPIEKKPLFHFLPGSLSLSLAAIGCNFKCLACQNWEISQAEELTA
                     KVNWGEDITPEEIVKIALKNNIPSISYTYTEPTVFLEYALDTMKLAKEKGLKNIWVSN
                     GYMSPEALELISPYLDATNIDLKGFNLAFYRKNCSATLEPVLNNLIAIKKKKIWLEIT
                     TLVIPGLNDDEESLKGIAQFIAQKLGRNTPWHISQFSGTISWKLQHIEDTPVETLEKA
                     YQLGRQAGLHYVYIGNVLGEEHENTYCPKCGTLVIGRQGYYITRKDIDGHCPSCHYNL
                     HIIN"
     gene            21772..>22388
                     /locus_tag="PK960_01570"
     CDS             21772..>22388
                     /locus_tag="PK960_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit B-like
                     domain-containing protein"
                     /protein_id="HPD07894.1"
                     /translation="MITFACLSPHAPIFLPEVGSPEDQEQVRDTIDSLESLGASLKKA
                     KPEIIIISSPHTDWGINVPLYFLDPPASFKVADYQTPDDIIIDTTRKSIIYPIITTLD
                     SPRQHYWWGQALYSDLLKNCSLNIALVGSGDMSHCLKIDGPYGFHPDGPKYDQALAEA
                     LKKKDLATILQLDELYPEAGECGLRSFSFILGILEAAGLNYQPEI"
BASE COUNT         7256 a         4762 c         3573 g         6797 t
ORIGIN      
        1 ctgattggcc gaggatataa ttatcactgg ctgccatcgg gtgttggttt agctagtttt
       61 ttggcggctt tcgcctattc cggggctggg ggaaacctta acctttccca atctttctat
      121 attaaagaca agggctatgg tatgggacta tatgcgcaaa aaataagcgg actatttagg
      181 tctaaccaag aacaggaagt taatttagag ggtccagact ttacacctaa ccctactaat
      241 ttacaacgtt ttcgacagtg gtggaaaaaa ataagcctag agcatttact agtattttgg
      301 tttttgggat tcctagctat ggccttgctg atgattttat catatagcac cgcttttggt
      361 cttaataata accaagaagg cattaacttt atttttaatg aagctaaact tattggacaa
      421 actctcgccc cctgggtggg ttcaattttt cttatacttc ttggtataat gctcgcccaa
      481 actcagctcg gcgtcctaga ttcgacctct cgcattatgg ccgaaaacgc cgccctactt
      541 aaactgcaga acaaaaaaga gaaaaaactt aatttgtcta aaatttatta tgcttttctt
      601 tggggacaga taatgtttgg cattatccta ttacttctta atttttatga gcctaaacag
      661 ctaattacga ttggagccat tattaatgcc atagcaatgt ttgtccatat tggatttgtc
      721 aatattggca actggaaatt attaccacca tccacccgtc cgggatggcc gcgaagaatt
      781 attatgctga ttatcttttt actctttgcc ggctttggtg gaattactct ttttagttat
      841 ctctaaaaat tattcagccc gtaaggcctc cactggatca agtttggccg ccttttgcgc
      901 tggccgaaaa ccgaacaaga gaccacaagc caaagaaaaa ataatagcta cgccaatacc
      961 ggaccaagga aaaataaagc tccattcaat gctaaaatga cgagccacga tacttaatat
     1021 ccaggcgaga aaaagaccaa taataatgcc aataacggac ccccaaatag taattagaac
     1081 tgcctccatt aaaaattgtt ctacaatatc atggtaatta gcccccaaag ctttacgcaa
     1141 tccaatctca ggggttcgtt cagaaactgt aacatacata atattcataa taccaatacc
     1201 gccaacaatt aaggaaataa tgacaatcgc tagcaataac caggtaactg tattagtcac
     1261 tgtatttaat gtcttcatca tttcagccat agtaataacc cgaaaatcat ctttatctgg
     1321 gtcattaata ccatgccgct cccggagcag tgaagtaatc tcctcagccg tatcatcggc
     1381 cttggttggg tctttcaatt gatgcaccat ataaagtata tagtctaccc ccaaaatgcg
     1441 tttctgtaga gtctttaggg gtagatatac tgacttatct atatctatct gactattacc
     1501 acctcgacct cttttttgca taacgccaat tacctgaaat ttattatgac caattttaat
     1561 ccacttcccc aggggtgatt gatcaccaaa taattcctct tttaaactat agccgattac
     1621 cgccaccatg gcttgattac ggtcttcttc ggccgtaaaa aaacggccct caatgggatg
     1681 acttttttga tctatttcca catagggcgc actaactcca aacagtagtg accgctcact
     1741 ctggccctgc caactcactt tctgctgtcc taaaatagca ccatagcttt gcttaacatt
     1801 ggccaattta ttcaccgcct ccatatcttc cactttaaga gtagttactt gagccaatgc
     1861 gctggctgtt ctctttttag gtaatctaat ttcagtttca atgatatccg taccataaga
     1921 agaaatttcc tcagtaacta aatttcttac tgcatcacca gcggcaaaaa ctacgacaat
     1981 agcggcaact ccaatcacca cccccaaaac agttaaggtg gttcgtaaaa gatttaaccg
     2041 taaagatcta atagataatt gtagggcggc atgaaacttc ataatatttt attcatagcg
     2101 taaagcatca actggctcta aattactagc tcgtctggct ggataaaggc caaaaatgat
     2161 accaatggca atagatatta atacggcaat aagtaaagct gggatagaga taacaaaatc
     2221 ccattgatat cgcaaaagat gagccaccac cgcggctaaa taagaaacta aaacaccact
     2281 aataatgcca atagaaccac ctattagcgt taaacatact gactccagca aaaattgtct
     2341 taaaatttgt ccattagtag ccccgatagc cttacgcaaa ccaatttctc tcgtacgctc
     2401 cgtaacgctt acaagcatta tattcataat gccaataccc cccaccacca aagacagacc
     2461 agccatagtc gctaaaaaat aattgatagt attagtaatt ctagtgatag tatccaggac
     2521 gtcctgaaaa ctatgcactg aaaaatcatc atcctctttt tttaaaatat tatgctgctc
     2581 ccgtagagtt tcctctattt ctaacactgc cctatctgca tcggcattag gaactaattt
     2641 tactcttata gaacttaaat aatctactcc ggcaatcatc ttttgagcaa agactactgg
     2701 cactaatatc tggtcatcat aatcttgcat tccaacttta cctcggcttt tgagcactcc
     2761 gattacttcc acagtttgat ttttaattct aagacgctgg ccgataggat ctacatcacc
     2821 aaacaactca tcaactacca cactacccaa tacggccaca tgagccgcac tgtccacctc
     2881 ctcattagta aaaaaacgac cctgctcaat agagctatcc tcaacatcaa aataattagc
     2941 tgtcacccca ttaattattg tctcataact tttatctctc cagataacat tagctgatgt
     3001 ctgataatac gccgctactg ctttaacatt agccacatta ttaggttcac ttaaggcttt
     3061 agcatcatca tactttaagg tagtgacggt tatacccaaa gtttcactag gtggtccccc
     3121 gtcggagtca cccggtaaaa caataactaa atctttacca aaacttttga gttgatcaag
     3181 aataagtgat tgagctccat taccaataga cataataata ataactgccg ctactccaat
     3241 aacaatcccg agcatcgtca aaaaactacg caccttatta ctcattaggg ctcgccaagc
     3301 taatgataat gattgtttga gctcttcccc catcttttta ttttaaaaac tcattctctt
     3361 tagcaatgcg ccgattaacc accggctcat cactaaccaa gcgcccatca taaatcttaa
     3421 taatgcgttg agcatgctca gcagtaaaag tttcatgggt aaccaagata atagtagtac
     3481 ctttatcatt taaggcctgc agtatttgca tcacctgtat gcccgactta gaatcaagat
     3541 tgccggtagg ctcatcggca aaaataacct gaggattatt aactaaagct cgagcaatag
     3601 ctacccgctg tttctcgcca ccagaaattt gattgcttaa ataataaaga cgatgtccta
     3661 atccgatatt ctctaaaacc tctttggctc gctcctccat tctcctctta tctttagaat
     3721 aaaggagtgg taattttaca ttatctaaaa ctgtagtgcg cggtaacaaa ttaaaggcct
     3781 gaaaaacaaa accaatactt ttattacgta aattggctaa ttcatcgtct gacaattcat
     3841 taacattatg tccattaaaa aaatattcac cactgctagg acgatctagg agccctaaga
     3901 tatgcattaa agtagatttg cctgaaccag atggacccat aatggaaaca aactcaccat
     3961 gcttaatctg aaaagtaata tcaaacaaag cttgagtaac taccccatca tttttatata
     4021 ttttattaag atgtcggaca tcaattaaag gataatcagc catattagtt atttaaattt
     4081 agttccagac cgttagactt tttggggtat tttataaagg ttaccacttg atcgccagga
     4141 ttaaccccac tggttacctc tactaaacct tcatcacccc gcaagcctac agttactggc
     4201 acttcttgta attgattatc ttttaatagc cgcacaattt tagttccatc atccttgtca
     4261 ataatcgccc gggctggcac cttaactaca ttatcacgct cagcagtaat aatagtgaca
     4321 ttagccgtca tgcccgcttt aatatcggta tgatattcag tggtcaacct agtaatttcc
     4381 tgcgaatcac taaaactaat ttttacttta taatatacca catcctgaat aactgtctga
     4441 gccgggtcaa taaaatatac ctggccatgg aatataacat caggcccaaa agcatctaaa
     4501 gtaatttcaa ccggattgcc aattttaatt ttagtaatat cggcctcagt aatatctgcc
     4561 tcaatataaa aattattgtt gaccaaaata gtaacaaagg gcttcccggc ggcagaaaac
     4621 tgctcaccca cctcataatt cacctgagta actactccat caagcggagc aactagacgg
     4681 ctatcatcaa tctgcttttg cacactagct aaattagcta aagcctgatt aatctgcccc
     4741 tgggctaaag ttatatcggc agctctggct ggagccttaa cattttctaa ttgggctttg
     4801 gctacttcta ctgccttagt agctgcatct aattgcgatt gggcgttagc aatttgtcgc
     4861 tctccggtta aacgcacagt atttaaatta ttttgcgctg cctgacgggc attatctaga
     4921 ttaaccttgg cttgatttag attatcttga gccgctgcta agctagtctc cctatttaat
     4981 ttagcattct tataactttg ggtagcggat tcagcagcag taatagcggc attaatttga
     5041 ttaatctgcc cgctaataat acttttgtaa ttatctaatt tggcctgaga aaaattattg
     5101 ctagtaatgg tagcctccaa cattgagtaa gcataagaca gggcttgagc tgtctgattt
     5161 aaaaattgtt tagttttatc agaggcaatg gtaaaggcat tatcactgaa cccatctcgc
     5221 gctgctgtca cggcttgact agcagcagta taactttcta aagctaaagt gcgagtatct
     5281 ttagtccgct ggagataaac actatcttta acgcttaaaa catatttagc gtcatcgtca
     5341 gtaagaatag tattaatgtg atctagggcc actttagccg caagaatttt atcgctaaaa
     5401 acaatcaaac cagagttata ggcattggta agattctctt gagtagtctt atctatgtta
     5461 gttaaattgg tttgagccgt agccactgcc tgctcttgaa ctgtagctgt ctcgggactt
     5521 ggatcagtaa gatcattaag ggttttttct gcttgtttga tattttcggc taccgtctta
     5581 ttaagcttat ctaactccgc ttgagccatt ttttgattag ccactgcctg ctccacactg
     5641 gcttggctta cagcaatagt ctgctggctt gcccctgata ataatttatt taaattggct
     5701 tgggcaattt gcagaccagc ctcagcctca ttttttctaa ggagtagggc actataatct
     5761 aactcaccca atagctgacc agccttaacg ctgtcaccaa ctttcacggc tacattgctc
     5821 acccgccctg atgttaaaaa attcaaagcc aactctttaa gaggtttaac cgttcccacg
     5881 acacttactg tttgttttaa tgggccagcc tcggcctgca ctgtggtata ttgcggcttg
     5941 ttttgtttgc tccctcgcca agcagcccaa cctaaaatta agcctaccac cagtactatt
     6001 ccaataataa ttaaccaagt cttcttatgt ttttgcataa aataaaaaca ataataaatt
     6061 atctgctaca atttatagca aataagtaat gttatattat atcataattt gtggtttggc
     6121 aatatcattg aaagtaagaa aaaccccgat gttaacatcg gggaaaagtt ataaaatcta
     6181 gtaagtcttt atgaaccggc caataacaat aatataagac cgatccgctc tcaaaaggtt
     6241 gccgccgatg gtggtttagt gggcaacagc tcatcgggca ttgagcgcaa cagatgtccc
     6301 aaattccttg gttaacttgt tcccaagaaa ttatataagt agtcggtacc cagccgccaa
     6361 tgatctgtct aaaaatatcc attcgtaccg cctctgacca cataacttct tcccaagaat
     6421 agtggaaaac cattttccag gctaattgct tattaatact atcatacaag gcctctggca
     6481 ttatcactac ctgagaaaaa atttcttccg gcaaccaatt taaaaacaag gtatgccagg
     6541 caccatggat tttccacggc aacttcttta agttgttttt agatgaatcc ccgccgcgac
     6601 tgcggggaat taaatggtga atagtcattt tattactact cacactttgc ctcctaaata
     6661 atttaataat gtactattta atagcataaa ttctttttaa tattttgtaa acccaataaa
     6721 aataagctaa tattacaaat atttaaaaca atacctataa ttgcttgtta gttctatggt
     6781 ttttctttat aataaaaaga aagcaccagc accaaacccc ttaagcaatt tattaatcat
     6841 gaattaatta tactaatttt attaaaaaaa ttttaaataa atctatggac ttaacttacc
     6901 agctagtaaa aaatgatcaa caaattaata gtttcatcaa aactaccgag aaatacttag
     6961 aacatttagg ttttactgac cacggtttta gacatctaac tattgtcgcc gataggtcta
     7021 aaaaattagc cagacgaata ggattaaccc caaaagaaca agaattagct gctgtcgctg
     7081 gctattgcca cgatttaggc aatttcatgg gacgcgaaat gcaccattac tggtcagctc
     7141 taattttttt ccaaataatg caaccccgaa taaaacagac agctgaccta gctactatta
     7201 tgcaggccat tgttaaccat gatagcaacc atctacaaac agataataaa attgccgctg
     7261 tcttaatttt agctgacaag tctgatgttc atcgaagccg ggtaagacaa aaagacctta
     7321 gcaaaattaa agaagacatt catgatcgcg ttaattatgc agtgactgat aatgatttat
     7381 tggtaaataa gaaggccaaa gaaataatct taagactcac catcgatact aaagaagttg
     7441 aacctattaa ttattttcaa atttttattg accgcatgac tcaatgtcag caggcggcag
     7501 aagtattggg atataaattt atcttaatta ttaataattt ccgcttctag cccaaaccag
     7561 gccacaaatt tatcaccaaa ccattaaaac ccaccaacca aatctccata aagccactgc
     7621 ttactagcag atttagatgt ttcttttgcc aagccataat tttgcctgct gattcattgc
     7681 cagtaaaatc taaaagtaat aataaatcgg ctttctgctc tggagtatat aatttagcct
     7741 tacgtttaat agttttagtt aaggcttgaa aattagtttt agttaattgc aaacgtaagg
     7801 gtcggccagt ctctccctgc tgataaacat aaacatccac ttcattttga aaataattag
     7861 gtcgcagaca attataactt gccttgttaa ctttattata tataggtaac catcgcttaa
     7921 cacactccag ctcactaatt ttttgccggt cattatgact ataatcaatg atatatttat
     7981 cctgttccat aattagtaat gataatcttt atgattattg atggtgagac cacggtatag
     8041 ctgctctaat aataaaactc gagccagttc atgcggccaa gttaataaag ataaagataa
     8101 tgtttcttta atttttagta tattgttatc tagccccgga gcaccaccca ttaccaaaat
     8161 aattaaccgg ccatcctgat cccagcggcg gagcatactt gcccacccca tagaatccag
     8221 ttccttgcct aactcagtta ataaataaac ctgcgcccct ggataatgct ccagaaactg
     8281 cgcaatttta atactatcat tatgacgtgc cactaaagcc tgacccggag caaatcgtcc
     8341 accagctaat tctatcaccc gaatctttgc tagttgaccc agccgctgat aataatcatc
     8401 ggctaattca cgacaagctg ctgacctcaa acagccgata gttactataa taatttgcaa
     8461 cattaaaaat tatttatcat cgccaggaga aattattaca ttctctcctg ccgaataacc
     8521 ttgattatct ctttctagat taaatattaa ccgatatccg ccattttcta ataaattata
     8581 caaatatggt tgactggtct taggatcact gggcatgggt gataagtaga gcctgcccga
     8641 tggacccaaa agtgactgct gaggctctgg cagattaatc ggatagctac cttcacggtc
     8701 tttatataat tccaaggcct gacgaatcat ataaatatca gctaagcgtc tagcatctcg
     8761 ccccttggcc ctagttccct caaggaccac ataagaaaga gtagaaataa tagcaataac
     8821 aaaaattacc actaataact ccaccaatgt aaaaccaccc cgagttttgt gctgagtcat
     8881 aataatttta gttataaaag ggatatttat cattatacaa aatttttctt taatttccaa
     8941 attactctac ttcagcaatt gacaatgata tgggaagata ataaaatgat aatgtcaaac
     9001 tgttgccacc gattttaatc ggcagaaggt caaaacccaa tcaggccagt aaggggcacc
     9061 cccttgctga ttgggttaaa aaccaaggca gcctggcaat agtttgacca ctaggggtgt
     9121 ccctttttgg taaaaattaa aaataccgaa gtttattcgg catttttatt tagattttaa
     9181 tctaaattaa ataatcggtt gtccatcagg aatatgacca agaatacccc aatctactgt
     9241 cttaacattg gaaaatttaa aaccatatcg caaaaagacc ttttgattta cgaatggcat
     9301 tttatgacca ttagcaatta aataaacagt ggtgccattg ggtgatttcg ccaaactata
     9361 atttttaaga ggacacttac cggttaattc gctagaatgt tttaccagtg taccatcttt
     9421 ataattcctt aaattgccgc tattatctat aacaatgtca ctagttttat aatttagata
     9481 cttaaaaacc gaccaattag ctagcggccg tttatattga ccatcaataa catatactgt
     9541 agggttcttg ggcgacttca ctgcctggcc atcatgataa gcaccccaat aggctggacc
     9601 agctttgtag aacgtaaaat atttatcagc gatagtttta gcaactgact tatcataacc
     9661 acgactaacc agactatcat catttaattt aattatttgc tgattatata aataataata
     9721 atcattaccg tgctttaaaa tatcaccgct tttaaaatat tggcgcaaat actcagctgt
     9781 aagtgaagcg gtcttaaaac tgtacacttg gctctcaaaa ctcttatgat taaggctact
     9841 agacattttt attttaaaat aataaaccgt gctaggattt agattggtta gttcagcccg
     9901 atggcttagc tgatggtcct ttgatgcttg cactctatta tttaaatgat taggatcaag
     9961 accataaact atctccgaag tagccagaac attactatcc cagctaataa ccaccgcctc
    10021 cggtaaaatt atggcctggt tggcattctc tggcactaaa ttactaattt ttatctgatc
    10081 atagttatga taggcatcgg tagtaaactc cccgcttctt tctgcaatat taccatcttc
    10141 atctttagct aaaatattaa aataatattt actacctgat gtaaggccat caataattat
    10201 taagtgctca taacgcaaag cactgtctga acgatgctta tctaatttat ctgaagtaaa
    10261 accataatag atatcaaagc gactcggctc attagtaaga aaataaattg ccgccgcagt
    10321 atctattgtt tgaaggaccc gaagcccagt gatctctggc gccgttgaat ctttcatttc
    10381 atcagtatta aaatattgaa tgaaagattc aactctctga ccactagaat tagtagccac
    10441 aattttataa tagtaatcag tcttcttttg gagaccagtc aaatcaattt catgactatg
    10501 tttaaactca atattaccta catgataaat taagcggtca ctactagtgc caaaataaat
    10561 atcactttta gtagcctcac tagtattcca agtaatagtg gctattccat cgtgaggttt
    10621 actaatagca atatctgtaa tggtcatctg agctgccgct ggcaacgtta tggccaaact
    10681 aattattagg agggtaggaa aaaataaata tttagataat ttcctaatta acataatatg
    10741 atcatcttac ggctgaaata aagtctctgg ttcaaacctc acctccttaa cctcaggaaa
    10801 ttgtttagct accgcctcaa gttgagccct gagaatagct acccgacagg ctccaccctg
    10861 agtgttattg tttttatcag acaatttaat agttaatatt ccactatctt tatctaaatt
    10921 aagactttct aaggaaacac cgggtaaagg aaattcgcta gaaagaccag caagtaaagc
    10981 cttttcgggt aaaggagtga ttaaaaaagc ctcaatagta tctttaattg gagtagtggt
    11041 aaaaggtata ttatagcccc aagccactaa ccccttcgga ctacataaaa tattaccgct
    11101 ctcatcttga tctaaccgac tatcataata aaaaaactta acggtccgct ccttgctctc
    11161 tggtgggttg ctagaaaaaa ctataggaat ccttaactcc ttggcctttt caggtaaacc
    11221 cgaagggtta tcattatgga caacgacaaa accagaacta gtggctggcg acttaaaact
    11281 aataactgct ctaaaatcta caaaatcatt agtcatccaa ttagattgag cctcggccgc
    11341 accttcgcct aattgctcgc cactagcatc gataattttt ataggaaaag aaccctcaaa
    11401 aaaccactga ccacgtgctt gaccaacaat cgtgactgga ctttctataa catctccggc
    11461 ccgaggttta tctaccacaa taaaatccga catatccaaa ggtttgttag attcaatagt
    11521 taaatgaggt tctttggcct taaacaaagt attaatctta ataaataaaa taattaaacc
    11581 acttattata acaagaccaa tgattaaata aaagattgtc tttttcataa attcattaaa
    11641 atttagcatt tttaggagtg cgggggaaag gaataacatc acgaatattg gctatacctg
    11701 tcaaatacat tataaatctc tcaaaaccta aaccaaaacc agcatgctta attgaaccaa
    11761 atcttcttaa atctaaatac cacccataat cttttttatt catcttcaat tcatcaatac
    11821 gctcttctaa tactgtcaga cgctcttctc tttgtccacc gccaacaatc tccccaacac
    11881 ctggtaccaa taaatcaact gctgctactg tccggccatc atcattttga cgcatataaa
    11941 aggctttgat atctttagga taatcagtca gaaaaaccgg ctgaccaaaa atattttcag
    12001 taatataacg ctcgtgctca gtttgcaggt ccattcccca acttactgga tattgaaaat
    12061 gctctttact ctttaagagt aagtcaatgg cttcagtgta tgataatcta ataaaattag
    12121 ctttaagagt cttctgcaga cgttccatta aagtatcatc aaccgaatca ttaaaaaatt
    12181 ctatctcctc tggcaaaagt tgtaagatat aatttactaa atatttcacc atctcttcag
    12241 ctaagtcagt aatatcagct aagtcagcaa aggccacctc cggttccacc atccaaaatt
    12301 cacaagcatg ccgggtggta ttagagtttt cagccctaaa agttggacca aaagtataaa
    12361 cccgattaag gccaaaaata agagcttcgg cctctagctg accactaact gttaggccta
    12421 ccctctgacc aaaaaaatca tgacggtaat caacctgacc ggtgggtgtt ttgataatag
    12481 gctgcgttaa atccatagtg gtaaccgtaa acatctcgcc cgctccttca cagtcgctac
    12541 tagaaataat aggagtatgc acatagctaa aaccgcgttc ctgaaaaaat tgatgaatag
    12601 ctgaacttag agctgagcgt acccgaaaaa ctgaagaaaa tgtagtggtc cttaccctta
    12661 gatgagctat ttctcttaaa aactctaagc tgtgcttttt cttttgaagt ggataactat
    12721 tatctgacag cccaataacc tcaatatgat cagccttgag ctcaaatggc tgaagaggag
    12781 ctggtgattt tactagctgg ccatcaactt taatagccga acccaggctt aatttttcta
    12841 tctctaaaaa attacttaac cctttatcaa aaacaatctg tagattcttt aaaaaagttc
    12901 catcattaag ctcaataaaa ccaaaatctt tcgaaagacg tactgtcctt acccagccgg
    12961 ctacagtaat attttggttt aaatatctat ctgtctcgcg atataaattt tttatatcaa
    13021 gaagattcat aaatcaaaaa ttaatactta ttagaactat taactcgata tttagtcatc
    13081 agattatcta aaatttgttt tagttgatag ggcttactaa tatgtttaag ctgaacccca
    13141 ccgtcaatag aaccagctgt agaaatagtt agcgagccgg tacctgtaaa aattttagta
    13201 aaaaacggct cttccaccgt aacattagta attttatcat aatcaatact cactaaatta
    13261 gtagatagcc agccacgatg caccaatacc cgatggtcag taaaagcata agcattggct
    13321 attttaaaat aaaaagcata aaagaaggta gctcccaaaa ataacaacag cggcaccaat
    13381 ggcgaccggt aaaccacaat cgatccaata ctaatcaaaa cccacaataa ccaataaaat
    13441 aaaaggtacc gattaccaac agaaaactga tactcaatct tttctccagg agtaattatc
    13501 ttttgccaaa tccgatcata attcatatat cttaatttat ctcaatctat cttataatac
    13561 actaaaaata aatttaacac tagctttgta tagtccaacc cccgtcaact acaagaatct
    13621 gaccagtgat atagcttgcc tctttagagg ccaaaaaagc caccgccgct gcaatatcct
    13681 ggggtgtacc taatcggccc aaaggtatct tcataataat ggctttctgg gattcttcgt
    13741 cagtattact agtacctgga gtcataatac ttcctggagc aacagcatta acggtaattt
    13801 gacgcggtgc tagctctaag gctaaagctc tcactaaact attagctccg cccttagagg
    13861 cgcaataatg agctaaacca gaaaaaccaa ggagtgaggc aatagatgaa atcataatga
    13921 tgcgtccgcc gtttgacata actcgggcta ctgcctgtga acataaaaat aaactcttta
    13981 aatttacgga tatcacctta tcccattgtt cttcagtcat gtccataaat ggttcaaagg
    14041 gataaatgcc agcattatta actaaaacat ctaacaaacc aaattcatcc attgtggcct
    14101 gaaccatatt ctggacatca taagaattag caacattagc ctcaattttt aaaactttaa
    14161 cccctagggc agaaatttcc cgggctgttt cggccagatt atttagatta atgtcagcga
    14221 tggcaatatt ataaccgtcg tgagctaatt gaaccgcaat accacgtcca attccctgac
    14281 cagctccagt aattaacgcc actctatgtt tattggatga cataagcaaa aattaaaaat
    14341 aaattttatt tattatatca taacaatttt taatgaattg taaaatatgt tatgatatgg
    14401 gaataagttt ttatatttag tctatgatct ctattgacaa agattctact aaaattgcct
    14461 atttcagcat ggagatagct ctaaacaatc aaatcaaaag ctatgctggc ggattaggtg
    14521 tcttagctgg tgatactctc aaatcagctg ctgacttaaa aatacccatg gtcggcgtca
    14581 ctcttttaaa tcgctatggt tatttcaaac aaaaaattag ttgtgagggg gaccaaatag
    14641 aattaccaga tacctttaat tatcgccatc ttaaaaaaac taatgtagta acccatataa
    14701 aaattggcga agatgtggtc aaaatccgtg cctgggaata tctcataagg gggcatagca
    14761 attattacgt cccagtatac tttctagata ccaatgttgc tggtaataaa gaaaagtacc
    14821 gtcgtcttaa tggtcaactc tatggagttg ggttagaata tcgtttaatg caagagattg
    14881 ttttgggccg aggcggcgta aaaatattgt ccgagctggg atatcacaat atccaaaaat
    14941 atcatctcaa cgaaggtcat accgcactag caacagtaga actatttcga gagtcactag
    15001 ctaaaagcag tagcgctaaa attaaagaag tgcgtcacaa atgtgtcttc accacccaca
    15061 cccctgtcaa agctggccat gatatttttc ctcttagtct agctaaggat ttacagcatg
    15121 atttacccca tcttccaaag ctatttcaaa atgacgagct aaatatgact aaattggctc
    15181 tttattttag ctcctatgtt aatggagtag cccgtagcca tcaagagata tctaaaaaat
    15241 tattccctgg ctattgtatt tattctataa ccaatggagt acattcagcg acctggactt
    15301 cgccagaatt tcagaaattg tttgataaat atatccctac ctggcgagag tgtagcctat
    15361 ccttacgcaa tgtctttagt attcctactt cagaaatatg gacagcccac caacaagcta
    15421 aggcccgact tcttaattat atttataaaa agcaaggcat taaactagat ctggatgttt
    15481 ttacgctagg ctttgctcga cgatttacag gttataaaag gtcaaccctc ctattttatg
    15541 atatgaatga attactaaga attcatcgta ctgctggccc catccaaata gtttatgctg
    15601 gtaaagccca ccaggatgac gaaaatggca agcaacttat taaagagatt attaagatta
    15661 aagaacaata taaaaaagac attaaaattg tctttttgga aaattatgat atgaatatcg
    15721 ccaaactcat gactgcagga gtagatgttt ggcttaatac ccccctcccc cctaatgaag
    15781 cttcaggcac tagcggtatg aaagcagctc ataatggtgt accccatttt agcactcttg
    15841 atggttggtg gatagaagga tttgtcaatc gtaaaactgg ttgggccatt ggagaacggc
    15901 gcactaccct tgaccctaaa caacttaata aacgtgacgc cgctaatcta tataataaac
    15961 tagaaaccag aatactgcct cgctactatc atgcccctga aaattggcga gaaactatgc
    16021 gttatactat tgctattaat gcttcattct ttaattccga aaggatgcta caacaatata
    16081 tccaaagcgc ttatttataa agtaaataat tttctaattt gttgatccac gctgggggta
    16141 aattatgttc tttggcaccg ggaatgactt tatcttttaa ataactataa ttgggttgtc
    16201 cgccggcctc ttttgatgat tctatgtaaa ctaaagcgga aacttcattg ttctcccatt
    16261 ctaccgtaac agtctgccgt ccatataatg taggatatcc ctcatatcta tctaacgccc
    16321 tcaaacaatc actatctata ttccataata atccccatac atattgacca gtatggggta
    16381 ctatattagc actaccccgt ctatcaaagg taaattcata atttggtaat ttagccggcc
    16441 ccaatgctgt aatattagaa ccacaacggc tatacatatt attgctactc agattagagc
    16501 cataagcaaa atataaaata gaagaggaaa cctggcttga ttgctctatg tcttgtctcg
    16561 gaacatgtcc taagaaaata actaatgcaa taacgaccaa taagcaaata acaaaaatta
    16621 ctaataataa attttttgac ggtaaagttt tcataaataa tataaattat actaataaaa
    16681 aataataaag aaaagacaca caggtctata gtctttctta actttgtctg atataattaa
    16741 ataataacga aaatattatc tttatgttat atttttttag aacgctcaaa ggactaaaaa
    16801 ttaaaattat taaaattggt tttataggaa tactattggc cgttattatt cctcttactg
    16861 tccaggcggt caccaataat caaggtactt tatctgacag ctcgctatct aacaatcagt
    16921 atttaaataa caacaatcct cttaattctg atgctattgc tgtccgtgtt tttaataatg
    16981 ataattattt aagtgcttat gattggtatc aatataatgt cagagctaac cgttcccttc
    17041 agtccctaat aattgatggc tatgaagcaa taagagacga ccgaactgtc tatgtggctg
    17101 cctccaatgt agtaacctgc agttccagta aacaatgctt acaacccatt attgccatca
    17161 tctcttttaa tcaaaatgtc tcctctgaga ctatagatat tttcggtcaa cttcttaaca
    17221 actggcgctt taaccaaaac attaataacc ctaagggtag ttgtttaagt aaacctgcca
    17281 ctacctctac tatttactgt ttgtctaata aagattgtcc cagcgggcaa tattgtacca
    17341 gtgctaaagc taaaatagta agagacacaa aacgtctaat taacctaaca tctatccacc
    17401 aaaaactaaa acagtattat gatacctatc atcattatcc caccctggaa gcaggaacct
    17461 atttaattaa caagaccttg tcggtctggc caagctggca gaaaactttg gccctaaaac
    17521 taggttcatc actacccata gatcccatta atcaattagg cccatgtcca gcagaatata
    17581 acccaattac ttgctggaat gaacagcggc gattatttgc cacaccctgg ccatctttac
    17641 caccagatag ctatacgtat caatatgaat atattgatag ccaccactac cgactttgtg
    17701 ctaattttga atcaaaaggc tatgccaatt tagccggact aaattgtcct caaaatcact
    17761 agaattttat ttagaccaaa tatagtggat aatcatggca ataataatga ccaacaaaac
    17821 taaacttccg atacttaacc aattagcaac accacgccat tggtcggctc taataaataa
    17881 ccaataccga ttagtaaact aataaaaata gcctcgccca ataagtcaca aacaataaat
    17941 tttctatagt cagtcttgga tagaccagcc ataagattaa ctactggccc aacccctggt
    18001 actaaaaagc gacttaaaaa aatagtaata gaagcatgtt tattaaaaat tttttcaact
    18061 ttaaaaaatt ttggagaatt aagcattcgt ctaaaaccaa tcttaactaa tacatctcgg
    18121 ccatagcaaa aagataaaaa ataagcagtt aggtctccca aaacgacccc acaataacca
    18181 actatccagg ttatggaagc gatcaaatac tttgagcgaa ttaaaaagcc cgaaaccaac
    18241 aaaatgggca tggctggagt aatattcaat aaagaggcca gaaaagtaat aataaataaa
    18301 gccagatatt tataagctat gattattgct aaaattttgg atagcataaa atgttaatag
    18361 ccttaactct agtgcaccat taaaatcttt aaaatattgt agctatttta tcaactaaaa
    18421 gcaatttttt ctccttggac aaattttgcc ctttacccct agtcagcttc tagggtcaca
    18481 aaattatatc tcttttttat ggcaatatta acataaatat gataatacta taagtctacc
    18541 ttaaggttga ctatccctag cctatctttt attatgactt tataataaga taaaaaaaaa
    18601 ctaaatttaa attttcagga tgactctttt ggctagcccg aaaataatta ctctaatcat
    18661 ttttatttta acctattgcg gtttaatatt tttgcccaaa aaacgggtac tctgcgcggg
    18721 tatcggggct ggtctactta ttatcttgca aattattaat cttccggaaa tcttctcagc
    18781 tattgattgg aatgttttac ttatgatttt aggaaccagc gggctagtaa tatttttttc
    18841 ggaatctaaa atgcctgctt ttcttgggga aaaagttatc gacaaggccc ctgactttag
    18901 atgggctgtt gtttttcttg ctcttttagc tgggggtatt agcgccttac tagaaaatgt
    18961 ctctacttta ctcatactaa caccagtagc cttagctatg gctaaaaaac taaaaacttc
    19021 tcctgtaccc ctccttatta ccattgccat ttcctctaac ttacaaggag cggctacttt
    19081 aattggcgat cccacctcca ttatgcttgg caattatgct aacctcaatt ttttacaatt
    19141 tttcttcctt aatcataaac caagcatatt ttttgctatc gaactgggag caattatgac
    19201 ggctatggcc atcttctacc tatttcgcaa agaaaaacag cccattacca ctgaagaaca
    19261 tactaaagta actaactttt ttccgactat tattcttctt ttaatgattg tctctctaat
    19321 tggtgtatct tttattaaag aaaaaccagc cctcactaat ggttttattt gctccggctt
    19381 ttttctctta gcagctttta gggaactaat aataaagcat aatttatccc ccaaaaaaat
    19441 tattaaaaac ttaaactggg aaaccttggt tcttttatct tttattttca ttatcattgg
    19501 cgctattgtt aaagtagggt taattagcga cattgctagc ctgattgcta gagctggtca
    19561 aggtaaaatt tttctccttt attctgttat tgtctggagt tcggtcctag cttcagcctt
    19621 tattgataac attccctacg tggctactat gctgccagta attgatgccc taacaatgca
    19681 gctaggcgta agcccttatc ttttttattt cggcctcctc tctggagcca ccttgggcgg
    19741 caacattacc cctattggcg cctctactaa tatcactgcc cttaatattt taaaaaaaga
    19801 aggctatacc gtctccttta aagaattcat aaaatatagt ctccccttta ctctagccgc
    19861 tgtcatctcc ggctatcttt tcatctggtt tttctggcat taaataagaa gttattggct
    19921 tactaataac tcaccaacaa aaaataatgg cccgcaaagt tacgggccat atagtattat
    19981 aatcctagta attcactaat ttttttgcca aattcctgag cagctgctgg accattagca
    20041 gtaataattc ggccatcaac tactacggcc tgatcaacat atttcgcacc attgtcttct
    20101 aaaacctgga tggaacttct atcttcaggg ctagaccaga ctgtggcctc tttatcttta
    20161 agcacaccag ccttagctaa aatgacaggt gagacgcaaa tagcggctaa aatttttcct
    20221 tggcctaaag tagattgcgc taattggtac gaattatcat tatctaaatt ctctaaactg
    20281 ccaggcccac caacaaaaat aacggcatca aaatcagctg gattaacatc agttataagt
    20341 aaatcaacat ttgcttctcg gccacccact cctcgggcta ccccagcttt attagaagca
    20401 gttttaacta ttaccccctg ctcctctaaa attgtcttgg gctcaaaata ctcttcatcg
    20461 cgaaaattct cactagctac aaccattact acttttttat tagacacact cactgaagtt
    20521 ggctgctcca ccactggagc aatggattga tttttattct tcttgctctt tggccctaaa
    20581 taaaaataaa gaccaatcgc caccagaaga ataataagta taacgatgga tgttctgctc
    20641 ataattttat cttaatatta ttaaaattct attgatatta tataaccaat attgcctttc
    20701 ttcaactcta atctttaaaa attaccttaa ttattatata atattattaa tgaaagaggc
    20761 ttatctttat gaaaaattaa agaaccataa tgtccgctgt ctaaattgca gccactattg
    20821 ccacttatct ccagaccaaa aaggtatttg cggtgttcgg caaaatattg acggtaaact
    20881 ctattcttta aattatggcc aggtcgtagc cttaaatatt gaccctattg aaaaaaaacc
    20941 gctgtttcat tttcttccag gctcactatc tttatctcta gcagctatag gatgcaattt
    21001 taagtgtctg gcctgccaaa actgggaaat ctcccaagcc gaagagttaa ctgctaaagt
    21061 aaattggggt gaagacatta cccctgagga aatagtaaaa attgccttaa aaaataatat
    21121 accctccatc tcctatacct atactgagcc aacggtcttt ttagagtacg ccctagatac
    21181 catgaaactg gctaaagaaa aaggcctaaa aaatatctgg gtgagcaacg gctatatgtc
    21241 tcctgaagcg ctagaattaa tctcgcccta tcttgatgcc accaatattg atttaaaagg
    21301 cttcaacttg gcattttacc gcaaaaactg cagcgccacc ctggaaccag ttttaaataa
    21361 tctaatagcc attaaaaaga aaaagatttg gctggaaata actaccctgg ttattcctgg
    21421 acttaatgat gatgaagaat ctctaaaagg catagctcaa tttattgccc aaaaattagg
    21481 ccgcaacacc ccgtggcata tctctcaatt ttctgggaca atttcctgga aactccagca
    21541 tattgaagat acgccagtag aaaccttaga aaaagcttac cagctgggac gacaagctgg
    21601 tttacactat gtctatattg gcaatgtttt gggggaagaa catgaaaata cttattgtcc
    21661 caaatgtggc acattagtta ttgggcgaca aggctactat attactagaa aagatattga
    21721 tggacattgt ccaagttgtc attataattt acatatcatt aactaaatca tatgattact
    21781 tttgcttgtc tatcccctca cgcccctatc ttcctcccag aagtcggcag cccggaagat
    21841 caagaacagg taagagatac aatagatagt ttggaatctt taggcgctag ccttaaaaaa
    21901 gctaagcctg agataattat tatctcctca cctcataccg actggggtat taatgtcccc
    21961 ctttattttt tagatccgcc tgcctccttt aaagtggcag attaccaaac cccggacgac
    22021 attataatag acacaactcg caaatcaatc atttatccca ttataactac tctggattcg
    22081 ccacgacaac attattggtg ggggcaggca ttatatagtg acctgctaaa aaattgctcc
    22141 ctaaatattg ctttagtagg cagtggtgat atgtcacatt gtcttaaaat agatggaccc
    22201 tatggttttc atcctgatgg ccccaaatat gatcaagccc tagctgaggc tctaaagaaa
    22261 aaagaccttg ccactatttt acagttagac gaactttatc ctgaggctgg tgaatgtggt
    22321 cttagatcat tttcttttat cttaggaatt cttgaggctg ccggactaaa ctaccagcca
    22381 gaaatttt
//
LOCUS       DAOOIS010000008        18403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_2983_length_18403_cov_32.948496, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000008 DAOOIS010000000
VERSION     DAOOIS010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18403
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2983_length_18403_cov_32.948496"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1333
                     /locus_tag="PK960_01575"
     CDS             77..1333
                     /locus_tag="PK960_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07895.1"
                     /translation="MQNLKFKLNSWSADFFWPLFIAAIFFLSPTLIIAWISFSRQDFF
                     QQHLENIGEAGCNLLVLAFCLYLLGYLLVAVIKPSANNSWLWLVAVLTFVVSLVTIPL
                     LSRDITAYALNADIFYHGHLNPYLTPIGLGRQSWRIGQPWWLNYPSPYGPAFFLLLGL
                     PFLVSWGQLLPFIFSYKVLVLLAFILTGYLFSSYRRQCHLPAYLDWLFYLNPALIVNW
                     LIEGHQEVFIVLILLWLLWASAKKSTFHPLGLLLIGFIKITAVILWPLSWFKDKIFNW
                     RRFWQANLLLLLSWLLFFAIIHLTPLDFWHNNLAFLDQQCFYACSPLVMVSNNLLGAG
                     AGIFRLGLFVILYLASLYFFLFKNYQPVKFIIWSFTALFFINTSWLTPWYPTLVIPFA
                     LLTKKRSYIILAGLITIYCLWHYVGL"
     gene            1363..2037
                     /gene="gpmA"
                     /locus_tag="PK960_01580"
     CDS             1363..2037
                     /gene="gpmA"
                     /locus_tag="PK960_01580"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HPD07896.1"
                     /translation="MYQVVFLRHGESIWNKEQIFTGWTDVGLSEFGRQQAREAGRKLK
                     ENKFVFDRGFISYLKRTRETMALVLEEMGLSIPVVADWRLNERHYGNLQGLNKEEMAK
                     KFGAEQVKIWRRSYDVRPPEITPDNPYNQQNDPQYRGIPVPSAESLADVVKRVVPFWK
                     EAVCPLLEKGERLIIVASGNSLRALVKYLDNISPADIVNLEIPTGQPLVYELDNALQP
                     LKHYYL"
     gene            complement(2040..2573)
                     /locus_tag="PK960_01585"
     CDS             complement(2040..2573)
                     /locus_tag="PK960_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07897.1"
                     /translation="MDQQKIKDVNEFIAKFDEMKKQDKMDLSADQDLSIAIMNLVSIE
                     EHLFFSGAKTGHHEYYDLINEIREVRKELLKKIITDYEGEVWCISKHLLAASYRLMEV
                     GNKELSSGKKDEAYDLFNKAYELYSLFWGLNMKLITTDDIKHIDDNAINKHDRSHKSL
                     AAKLGDLVKRAINCCIE"
     gene            complement(2575..3090)
                     /locus_tag="PK960_01590"
     CDS             complement(2575..3090)
                     /locus_tag="PK960_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07898.1"
                     /translation="MKLSRLTPATLIVAIILAIKPIIARAVCPVCTIAVASGVGLSRY
                     LGIDDIITGLWIGGLIVSMIMWTIGWLQKKHISFPGRNIIIILAYIALVVLPLYFSGF
                     FSHEASCVCGINKLLLGIINGALGFWTGAEWYTYLKIHHGNKAYFPFQKVVMPLLPLV
                     ILSLLFAQLIK"
     gene            complement(3087..3500)
                     /locus_tag="PK960_01595"
     CDS             complement(3087..3500)
                     /locus_tag="PK960_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07899.1"
                     /translation="MNYRVIIASGLFLAVTIFSCYALTKHQTSNNHPDSQPATASQRQ
                     PAADFILYYGETCPHCHIVTNYIATSKIREKFQLLEKEVYNNETNARELTTVAHDCGL
                     SQNNIGVPLLWDGHQCIIGDQPIIDFLNNKLNQVP"
     gene            3986..5794
                     /gene="thrS"
                     /locus_tag="PK960_01600"
     CDS             3986..5794
                     /gene="thrS"
                     /locus_tag="PK960_01600"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPD07900.1"
                     /translation="MPNYPLEIKRHSLAHVLAAAVTELYPQVRLAIGPAIDNGCYYDF
                     DFGPIKLSDEALGVIQNKMLEIIASPLNFERSVKDIDEALAKEKQQGQIYKAELIADL
                     KKQGITEVSYYTLGQFTDLCRGPHVDSTADLRGLGFMVHHLAGAYWRGDEHNPMLTRI
                     YVLAFETPAELDHYLQVLSEASERDHRKLGRELDLFHIDEEVGLGLPLWHPKGAILWK
                     TIEDFWSTQHLRHGYTLVRTPHIGSKSLWETSGHWGFYSTSMYPPLEAGQTLRDKQQG
                     IKVKTSEEYLLKPMNCPFHLEIYREKLRSYRELPLRYAECGTVYRYEKSGELSGLVRV
                     RGFTQDDAHIICRVDQVEAELKGVIDFILFMFQSFGFDIHSINVYLSLRDPKDNIKYA
                     GSNEGWEFSERILKKVAQEKNLNFKEEIGEAAFYGPKLDFKLKDVLGREWQCSTLQFD
                     FNLPERFNLTYINSEGQPERPYILHRALFGSFERFIALLIENYAGAFPVWLAPVQAKI
                     VAVSDKYYDYSNSLAKILIDHDIRVEIDKNEETVSNKIRKAIHDKIPYTLVIGEKEVN
                     SGALTIRQRGSTELLTMSQDEFITFIEGKIKDHQLI"
     gene            5814..6422
                     /locus_tag="PK960_01605"
     CDS             5814..6422
                     /locus_tag="PK960_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07901.1"
                     /translation="MKKKLLWLLIIAFIVANILAAIKVSQTHKSQGVNEVTSSLETTT
                     APTINFVTPEVAPASPTITKEDIARIACHEPLSLDDWPVEVDNNLSLAPVNWQNRRVN
                     LTDKKVVEQSYRAGINLAGHFHLATWSCGENCELAAIINAKNGQVIAFGADDDLRSVG
                     GWQFSPTSSLLIINPGSKDQNNPTIYSLVEEKGLRRICYLAQ"
     gene            complement(6424..8250)
                     /gene="uvrB"
                     /locus_tag="PK960_01610"
     CDS             complement(6424..8250)
                     /gene="uvrB"
                     /locus_tag="PK960_01610"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPD07902.1"
                     /translation="MQFQLQAQFQPTGDQPQAIQKLSQGIQQGLKAQTLLGVTGSGKT
                     FTIAKVIEKTQKPTLVISPNKTLAAQLYKEFKAFFPQNAVHYFVSYYDYYQPEAYLPP
                     TDTYIEKEATINEDIDRLRHAATQALLTRRDVIIVASVSCIYGLGEKTDYEASSQIFT
                     QGQTINRQEIIKELIAMQYERQDAACHRGQFRVAGDIIDIYLASGEAIVRLDLYDEII
                     NSIYLWPLNPDNNKPRPLRLSDLQLTEAQKLTEIHILPAKHFMTPHDRLETASAAIRQ
                     ELKERIQELQNQGKLLEAERLERRTNYDLEMLEQVGYCNGIENYSRHLAGRPAGSPPA
                     TLLDFFPDDFLTIIDESHITIPQITGMFAGDQARKEVLVEFGFRLPSAMDNRPLNFTE
                     FNHKIKQVIYTSATPGPYELKHSQQIVEQIIRPTGVLDPAIEVRPTHGQIADLIKECQ
                     LRAQREEKVLITTLTKKMAEELADYLKEAGLKTNYLHSDVDTLERIEVLQGLRRNKYD
                     VLVGVNLLREGLDLPEVSLIVILDADITGFLRNETSLIQTIGRAARHINGQVIMYADT
                     ITPAMNYAIKETERRRAIQAQYNKEHGLRPQAIKNAEWSNFD"
     gene            complement(8349..9728)
                     /locus_tag="PK960_01615"
     CDS             complement(8349..9728)
                     /locus_tag="PK960_01615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPD07903.1"
                     /translation="MMIMVFTSLGAQTTPHQLWAMPLDIIGIYCYNMEIIGDNIFLMY
                     LSGPYPYPQTLIQVNYTTGEIGWTRAGFIGNEVVHKKQLGVTSDNNLVTVDERHIAYK
                     ISAQDGSTIWTQDIFPSLHQQTPWWTIYTFPEYIAYLNVWMGWVCLDNTTGNIIGNLG
                     LGDDWIAPEWGGDIYSADGYNIYICGMINDGTFNGNIRKETLTTNNGLINGVSLNWQC
                     TIWDKVGRYIIPNNNKLYICTATIDFNTLNEATWWMQQLDLDTGEIFWEHYIGTAEEY
                     QPEGMINNGSNILFYGSHTTSSGTVPILMNYDANGQLTWTFSPQLPPGTNGTYISARW
                     HGNTLVLLSTVPSIRLSGISTGTNPVDDTTTPPIINSVYCYPNPFRDNTNIEFRQEKA
                     SEQTTIAIYNVKGQLVRTLAQQPFAVGEHTITWDSKDDAGQKVSPGIYFYKITSGRFS
                     ATKKVILLK"
     gene            9886..10278
                     /locus_tag="PK960_01620"
     CDS             9886..10278
                     /locus_tag="PK960_01620"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPD07904.1"
                     /translation="MKIIKLTKFYHQAFTLVELLVVIAIIGILSTLSIVGLNSARAKA
                     RDSRRLSDVREIATALEMYYSDTGAYPFVASATGTLIGGLCLSDLGISSTCGTSVYLK
                     KSLTILRVGPIIGICLVAGGRATRLIFA"
     gene            10233..10973
                     /locus_tag="PK960_01625"
     CDS             10233..10973
                     /locus_tag="PK960_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HPD07905.1"
                     /translation="MSSSRGASYQINFRLEQGSQGYPATSLVMVPGGIQESLYYGLIG
                     WWPFNEGSGLLTSDASGHNNQGIISGALWQPHEPGWSLLFDNVDDGVNIPTNNFVGLY
                     EYTMSAWVKPVGTHKNYTGTIISSGNWNSIHWAFGLGQNNEYIKFNRADGSRWVSFSY
                     NVPLNQWTHVAVTRFNDKLNYFVNGQYIGQSSISASGELNSNATNTMIGRETYANGYF
                     DFNGAIDDVRIYNRALSSEEIAILAQQN"
     gene            11042..14053
                     /gene="ileS"
                     /locus_tag="PK960_01630"
     CDS             11042..14053
                     /gene="ileS"
                     /locus_tag="PK960_01630"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPD07906.1"
                     /translation="MDQPSSFFAQSELEVLAFWDRAKIFEQSVEKTALHGDYRFYDGP
                     PFATGTPHYGHIVASIMKDVVPRFWTMKGYRVERRWGWDCHGLPIENIVEKELGYRHK
                     KDIEALGIDKFNELCRSKVLGYVNEWRTIIHRLGRFVDMDNAYLTMDLSFMESVWWVF
                     KELWDRGLIYHGYRSMYICPRCETTLSQSEVAEGYKDAKDLSCTVKFKLQPNQKIGDF
                     VINDSTYILAWTTTPWTLMANVALAVGADMDYIVVEIPANFDFKQFNVDLDLTGRYIF
                     SPQCAAQNDKLHQLLELALQGHVPSYKFKGHDLVGLKYLPPFDFYYSQSDLANKDKGW
                     QVYPADFVNTEDGTGIVHIAPGFGEDDWQLAKQYDLPFVQHVKMDGTIKDEAGEWAGL
                     NVKPKEDYQATDKKVVEFLGKSGLLFSQEEYEHSYPYCWRCDTPLINYATSSWFVNVT
                     KIKPLVLEEAKKINWSPSYIKEGRFGQWLEGAKDWSISRQRYWASVIPIWQCSCGWQK
                     VIGSVQELAELSGKEITDLHKHIVDKITIKCPNCGKLVHRVPDVLDTWFDSGSMPYAQ
                     AHYPFENAENFDSHFPAQFIAEGIDQTRCWFYYLHVVSTALGRGVAFRNVIVNGIVLS
                     EDGKKMSKKLHNYPDPAALMDKYGADALRYYLLASPVTQAENLNFSETGVEEAVKKII
                     LPVWNVYKFYDLYGGNERSVLSREDWHSDNILDRWIVARLHQLLGDIDSAMMDYNLPK
                     AVRPLAIFIDDLSSWYLRRSRNRFKSGESQDKAAAILTTSYVLQHLAIALAPFTPFLG
                     EALWQKTTGFNYEHDDKSVHLLNWPTDLPVDERVLTEMAVVREIAELALAERDEAGLK
                     IRQVLPSLTIKGPTDRLSLLTSASLDILADELNVKHIVTETNNSAGWEVKLDTTLTPE
                     LIREGLARELVRHINLLRKELGLTLSNRTIIYWSSTSPLANLTLEEWAATIQKETLSA
                     KLEEGQPDQFLAHKTLNLDDGTLEIMIAKLN"
     gene            14077..14640
                     /locus_tag="PK960_01635"
     CDS             14077..14640
                     /locus_tag="PK960_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPD07907.1"
                     /translation="MLVLYILLIIIAIFIIWLIAVYNGLIASRNRLAEALSDIDVQLK
                     RRYDLIPNLVETVKGYAAYEQETLNKIIEARNEAMSAHKTGDKEGEKAAEDNLSGLLK
                     NLFALSENYPDLKANQNFLELQRELTDTEDKIQAARRFYNSNARDFNIKLQVFPTNLV
                     AKKLGFTPVEFFEITDKAEKEQVKVEF"
     gene            14645..15553
                     /locus_tag="PK960_01640"
     CDS             14645..15553
                     /locus_tag="PK960_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPD07908.1"
                     /translation="MNLYTHISANKRRSWLLIIINGVLLLALGWVLSQATNDYIWFYI
                     AVIYSLVSSLVGYLWGDKVVLMTSKAKLVDRKDAPELYRLVENLCIAAGLPTPKIYII
                     NEAAPNAFATGRDPKHASVAVTSGLLAKLDKAELEGVIAHELSHVGNRDILLATLVSV
                     VVGAVALLGNWFARWSFWGGNRRSSNNDEAGQISVILSIIALVLAILAPIVAQLIQLA
                     ISRRREFLADADGVLLTRYPEGLINALKKISADPQLLSVASEATAHLYFASPFDNKKR
                     TPLLAKMFMTHPPVEERIAALEKINI"
     gene            15555..15860
                     /locus_tag="PK960_01645"
     CDS             15555..15860
                     /locus_tag="PK960_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07909.1"
                     /translation="MANPDLPKDLSKTENIAASGLKEVWRAEEQEHRQRKIFLIIGIL
                     LVIIVIGGLGFMSWPYVQGQIYGYYDKISKEIKSWPAKYAAWQKKNEEAVIKGDVFK"
     gene            15880..16452
                     /gene="ruvA"
                     /locus_tag="PK960_01650"
     CDS             15880..16452
                     /gene="ruvA"
                     /locus_tag="PK960_01650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPD07910.1"
                     /translation="MLAFIKGEIKAKRLGSIIVENNNLGYQIMVGEKIWEEAKTGETI
                     ELFLHHHLREDSDELYGFADLETLSLFELLLSVSGIGPKSALNILSLADIKDVQQAII
                     LGDEVMLTKVSGIGRKTAERVILELKNKIASLPANLLAGEEGSSNEDDLEALIALGYS
                     APAARRALAKVDRKITDPAERLKLALKELP"
     gene            16456..17418
                     /locus_tag="PK960_01655"
     CDS             16456..17418
                     /locus_tag="PK960_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPD07911.1"
                     /translation="MWQIIDSLEADDFVKHYGASEFLQSSAWLDIQRQFYSVETLGYY
                     LDKELMSVARLIRRPLGGRFCYYYSPRGPIVRKTHYNLWPQLIKDLGPWLKERRALFW
                     RLEPGSNWLDYDFASLGFYHGPDIQPSETRVLSLDSSPEELFSRMHPKTRYNIRLASK
                     RGVTVRRAGPQDFASLWSLLLLTSQRDNFHLHRREYYLALINSPAIELLLAEYHGQVL
                     AAGIFAYWGDTAIYLHGASGNEHRELMAPYLVQWQAIQLAYELGARRYDLFGTSSQKW
                     PGITRFKQGFGGEIVHYPGTFDWPLSKWNYNIYATLRQWRRKKH"
     gene            17427..>18403
                     /locus_tag="PK960_01660"
     CDS             17427..>18403
                     /locus_tag="PK960_01660"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPD07912.1"
                     /translation="MNKFFAQLKKTIYNSYLKHLVPAYHFIVNWLADVYYNHPSRQLI
                     VIGITGTTGKTTSVLLTAEVLRAAGYKVGYTSTAMFSDGQRDWLNDTKMTMVGRFFTQ
                     KMLRQMVNNGCHYAIIETTSQGILQYRHRFINYDLTIFTGLYPEHIEAHGSFDNYRRA
                     KGRLFSHLRRCGYKYVGSDKHVEKVDHGFKKVGLDKVPKTIVANLDDDYAHYFLSFGA
                     DKAWGYTTNYKEKKDLEWPDYLIMLEYGQIKSDIHGTDFICEGQEIHLPLLGAFNASN
                     AMNAIGVALSQGIDWPVIKAGLGAVKVLAGRLEIIDEGQPFSVIVDYAF"
BASE COUNT         5180 a         3108 c         4051 g         6064 t
ORIGIN      
        1 ttgttggtta ttaatactct cggtctttta gagatacttt atatttttgt ctttagtaaa
       61 ttagggcaaa agaccgatgc aaaacctaaa gttcaagcta aatagttggt cagctgactt
      121 tttctggccg ctatttatag cggcgatatt ttttcttagt ccgactttaa ttattgcctg
      181 gatatctttt agtcgtcagg attttttcca gcaacattta gaaaatattg gggaggctgg
      241 ctgtaattta ttagttcttg ctttttgtct ttatttattg ggctatcttt tagtagcggt
      301 gattaagcct tcagctaata attcctggct gtggttagtg gcagtattaa cttttgtcgt
      361 tagtttagtg accattccgc ttctgtctcg tgacattacc gcttacgccc taaatgccga
      421 tattttttat catggccacc ttaatcctta ccttacgccc attggcctag gacgtcagtc
      481 atggcgcata ggtcaaccgt ggtggctcaa ttatccttcg ccctatgggc cggccttttt
      541 cttactctta ggattgccat ttttagtgtc ttggggacaa ttactgcctt ttatttttag
      601 ttataaagta ttggtgttac tggcttttat cttgactggc tatctcttta gttcctatcg
      661 acgccaatgc catttaccag cttacctaga ttggttattc tatttaaatc cagccctgat
      721 tgttaattgg cttattgaag gtcatcagga ggtgtttatt gtcttaattc ttttgtggtt
      781 attatgggcc agtgctaaaa agagcacttt tcatccattg ggattattac ttattggctt
      841 tattaaaata accgccgtca ttctttggcc actaagttgg tttaaagata aaatttttaa
      901 ttggcgacgt ttttggcaag ctaatttatt attgctttta agttggctat tattttttgc
      961 tataattcac ttaactccct tagatttttg gcataataat ttggcctttc ttgatcagca
     1021 atgtttttat gcttgttcac ccctagttat ggttagcaat aatctgttgg gggcaggcgc
     1081 cggtatattc cgtctaggat tgtttgttat tctctatttg gctagtcttt atttcttttt
     1141 atttaaaaac taccagccag tgaagtttat catttggtct tttactgctt tattttttat
     1201 taacacgtct tggcttactc cgtggtatcc gactttagtt attccttttg ctttattaac
     1261 taaaaaacgc tcttatatta ttttggctgg acttattact atttattgcc tttggcacta
     1321 tgttggttta taattatata taaatgattt atttgataat ttatgtatca agttgttttt
     1381 ttgcgccatg gtgaaagtat ttggaataaa gagcagattt ttactggttg gactgatgtc
     1441 ggtttaagtg aattcggccg acagcaggcc cgggaagctg gccggaaatt aaaagaaaat
     1501 aagtttgttt ttgatcgcgg ctttatttct tatttaaaac ggaccagaga aactatggct
     1561 ttagttttag aagaaatggg gttatctata ccggtagtgg ctgattggcg tcttaatgaa
     1621 cgtcattatg gtaatctgca agggcttaat aaagaagaga tggccaaaaa gtttggggct
     1681 gagcaggtta agatctggcg tcgtagctat gatgttcgtc ctccagaaat aacaccggat
     1741 aatccatata accaacaaaa tgatccacag tatcgaggta ttccggtgcc tagcgctgaa
     1801 agtttagctg atgtagtaaa gcgagtggtg cctttttgga aagaggcagt ttgtcctctt
     1861 cttgagaagg gggagcgtct tattattgtt gctagcggta atagcctaag agctttagta
     1921 aaatatttgg ataacattag tccagctgat attgtgaacc tagaaatacc tactggccag
     1981 ccgttagtat atgagttaga taatgcttta cagccgttaa agcattatta tttgtaagat
     2041 tattctatac agcaattaat tgcccgtttg actaagtcgc ccaatttagc ggctagactt
     2101 ttgtgactcc ggtcatgttt atttatggca ttgtcatcaa tatgtttaat gtcgtccgta
     2161 gtaataagtt tcatgttaag gccccagaaa agggaataga gttcataggc cttattaaat
     2221 aggtcataag cttcatcttt tttgccggat gatagttctt tgttacctac ttccattagc
     2281 cgataactag ccgctagtag gtgtttggaa atacaccaca cctcaccctc atagtcggta
     2341 atgatctttt ttagtaattc tttacggact tctcttattt cattaatgag gtcataatat
     2401 tcatggtggc cagttttagc gccggagaaa aagagatgct cttcaatact tactaaattc
     2461 atgatggcaa tacttaaatc ctgatcggcg gataggtcca tcttgtcctg ttttttcatc
     2521 tcgtcaaatt tagcgataaa ttcattaaca tctttaattt tctgttgatc catattattt
     2581 aattagttga gcaaataaga ggctaagaat aacgagcggt aaaaggggca taactacttt
     2641 ttgaaaggga aaataagcct tattaccatg atggattttt agatatgtat accattcggc
     2701 cccagtccaa aaacctaggg ccccattaat gattcctaat agtagtttgt tgataccgca
     2761 gacacaactg gcttcatgac tgaaaaaacc agagaaataa agaggaagga cgactagggc
     2821 aatataggca aggataatta taatattacg gcccggaaag ctgatatgtt ttttttgtag
     2881 ccagccaata gtccacataa tcatggaaac aattagacca ccaatccaca ggccggtaat
     2941 aatgtcatca atacctagat agcgggaaag tcctacacca gaagcgacag caatagtgca
     3001 gactgggcag acagcccggg caattattgg ctttatggcc aaaataatag cgacaattag
     3061 agttgctgga gtaagacggc ttaatttcat ggtacttgat ttagtttatt atttaagaaa
     3121 tcaataattg gctggtcgcc aataatacac tgatgaccat cccagaggag gggtacacca
     3181 atattatttt gagaaagacc gcagtcatgg gccacagttg ttagttctct ggcattagtc
     3241 tcgttattat atacttcttt ttctagaagt tggaattttt ctctaatttt gctagtagca
     3301 atatagttgg tcacgatatg gcagtgaggg caagtttcac cgtaatagag aataaaatca
     3361 gctgccggct gtctttggct agcagtagct ggttgggagt cagggtggtt atttgacgtt
     3421 tggtgcttag ttagggcgta acaagaaaaa atggtgacgg ctagaaataa tccgctggca
     3481 attattactc tataattcat ataaattatg ctagataaat attattgtag cacaaatctg
     3541 tttaatgacc aaatacgtta aagattataa aaaacgacaa gcaaagcttg ccgttgaggt
     3601 tattgagaga taaaatcgtg gagggcgaaa gcaggctata atgacaagcc ccggaacttg
     3661 gttcaagttt tagaaccgtc cgttgctctt agacgccctc ctgtagattg ctaaagtaca
     3721 atccttataa ctaaggtact attttttaaa aaaatgtcaa gccttgacct cttggcttaa
     3781 aaatattatt attatgaggc gatgattgcc gcgtctacca agcgcggggt ataaccggga
     3841 atccctaagt cgttaattaa gtgcccattc atgggaagct agatggaacc gcgagctaaa
     3901 aaactcgttc tagcaataaa tattagtagt ttattgttgg aatgagtttt tatttaggtt
     3961 aataaaaaag aataaaaata gatttatgcc taattatcct ttagaaatta aacgtcattc
     4021 gctagctcat gttttagccg ctgcggtcac ggagctgtat cctcaagtgc ggttggctat
     4081 tgggccggcg attgataatg gttgttatta tgattttgac tttggtccaa ttaaattgag
     4141 tgatgaggcg ttaggagtta ttcaaaataa aatgctagaa attattgctt ctcctttaaa
     4201 ttttgagcgg agtgtcaagg atattgatga agctttagct aaagaaaagc aacaggggca
     4261 gatatataag gcagagctta ttgcagattt aaaaaaacag gggataactg aagttagtta
     4321 ttatacttta ggccagttta ctgatttatg tcgtggtcct catgtcgatt ctactgctga
     4381 tttacggggc cttgggttta tggttcacca tctagctgga gcctattggc ggggagatga
     4441 gcataatccg atgttgaccc ggatttatgt gttagccttt gaaactccag ccgagctaga
     4501 tcattattta caagttttaa gtgaagctag tgaacgagat catcgtaaat tggggcggga
     4561 gcttgattta tttcatattg acgaagaggt ggggctaggg ttacctcttt ggcatcctaa
     4621 gggcgcaatt ttatggaaga ctattgaaga tttttggtca actcaacatt tacggcatgg
     4681 ctataccttg gtgcgcactc cacatattgg cagtaaaagt ttatgggaga cttcagggca
     4741 ctgggggttc tatagtacta gtatgtatcc gccgttagag gctggtcaga cgctccgaga
     4801 taaacaacag ggcattaagg tgaaaacttc tgaggaatat ttacttaaac ccatgaattg
     4861 cccttttcat ttagagattt atcgagaaaa attaaggtca taccgtgagc ttcctttacg
     4921 gtatgctgag tgtggaaccg tgtatcgtta tgaaaaaagt ggggagcttt ctggattagt
     4981 gcgggtacgt ggttttactc aagatgatgc ccatattatt tgtcgagtgg atcaggttga
     5041 agcggagctt aaaggggtta ttgattttat cctttttatg tttcaatctt ttggttttga
     5101 tattcactct attaatgttt atttgtcttt acgtgatccc aaagataaca ttaaatatgc
     5161 gggttcaaac gagggttggg agtttagtga gcgtatttta aagaaagtag ctcaggagaa
     5221 aaatttaaat tttaaggagg aaatagggga agcggctttt tatggcccga aattagattt
     5281 taaattaaaa gatgttctgg gacgggaatg gcagtgttca accttacagt ttgattttaa
     5341 tttaccggaa cgttttaatt taacctatat taactctgag ggacagccag aacggcccta
     5401 tatattgcac cgggctttat ttggttcttt tgaaagattt atcgccttgc tgattgaaaa
     5461 ttacgccggg gcttttcctg tctggttagc accagttcag gctaaaattg tcgctgttag
     5521 tgataaatat tatgattata gtaatagttt agctaaaata ttaattgatc atgatattcg
     5581 ggtggaaata gataaaaatg aggagacagt gagtaataaa attcgtaaag ctattcatga
     5641 taaaataccc tatactttag taattggcga gaaagaagtt aattccgggg ctttaactat
     5701 ccgacaacga gggtcgactg agcttcttac aatgagtcaa gatgaattta ttacctttat
     5761 tgagggaaag attaaagacc atcagttaat ttaatgagtt tttaatataa attatgaaga
     5821 agaaattact atggctttta attatcgctt ttatagtagc taatatttta gcagctatta
     5881 aggtaagtca gacccataag tctcaaggag tcaatgaggt aacatcatct ttagagacaa
     5941 ccactgcgcc gactattaat tttgttactc ccgaagtagc cccagcaagc ccaaccatta
     6001 ccaaagagga tatagcccgt attgcctgtc atgagccttt gtctttagat gattggccgg
     6061 ttgaggtaga taataatcta tctctcgccc cagttaattg gcaaaatagg cgtgttaatt
     6121 taactgataa aaaagtggtc gagcagtcat accgggccgg tattaattta gctggccatt
     6181 ttcatttagc tacttggtct tgtggggaaa attgtgagtt agctgctatt attaatgcga
     6241 aaaatgggca agttattgct tttggtgctg atgatgattt acggtcggtt ggtggttggc
     6301 aattttctcc caccagtagt cttcttataa ttaatccagg aagtaaagat caaaataatc
     6361 ctactattta tagtctagta gaagaaaagg ggctaagacg tatttgctat ttggctcagt
     6421 aatttagtca aaattagacc attcagcatt tttaatagct tgaggtcgga ggccatgttc
     6481 tttattatat tgggcttgga tagctcgccg tctttccgtt tctttaatgg cataattcat
     6541 ggccggtgta atagtgtcag catacataat gacttggcca ttaatatgtc gtgcggctcg
     6601 gccgatagtt tgaataagcg aggtttcatt tctaaggaag ccagttatat cagcatctaa
     6661 aataacaatc aaggaaactt ctggtaggtc taagccttcc cggaggagat tgacccctac
     6721 taaaacatcg tatttattac gacgtaggcc ttggagcact tcaattctct ctaaagtgtc
     6781 tacatcacta tgaagataat tagtttttag gccagcttct ttaaggtaat cggcgagttc
     6841 ttcggccatt tttttggtga gagtagtaat cagtactttt tcttctcttt gagcccgtag
     6901 ttggcattct ttaataaggt cggctatttg tccgtgagtc gggcggactt caatagccgg
     6961 gtctaataca ccggtcgggc ggatgatttg ctccacaatt tgttgagagt gcttaagttc
     7021 ataaggacca ggagtagcgc tagtataaat aacttgtttt attttgtgat taaactcagt
     7081 aaaattgagg gggcgattat ccatagcgct tggtagtcgg aagccaaatt ccactagcac
     7141 ttctttgcga gcctggtcgc cagcaaacat accagtaatc tgtgggatag tgatgtgaga
     7201 ttcatcaatt atggttaaaa aatcatctgg aaagaaatct aggagagtgg cgggcgggga
     7261 accggcagga cggccagcaa gatgccggga ataattttcg atgccattac aatagccgac
     7321 ttgttccagc atttctagat cataattggt acgacgttct aatcgttcag cttccagtaa
     7381 tttgccttga ttttgaagct cttggattct ttctttaagt tcttgacgaa tagcggctga
     7441 agctgtttct aatcggtcgt gaggggtcat aaaatgtttg gcaggtagga tatgaatttc
     7501 tgttaatttt tgtgcttcag ttaattggag atcgcttaaa cgtagaggcc gcggcttatt
     7561 gttgtcgggg ttaaggggcc atagataaat gctattaata atctcatcat aaaggtccag
     7621 gcgcacaata gcttcgccac tcgccagata gatgtctata atatcgccgg ctaccctaaa
     7681 ttggcctcga tggcaagcgg catcttgccg ctcatattgc atagcgatga gctctttaat
     7741 tatttcttgc cgattaatgg tttgtccttg agtaaatatt tggctcgacg cttcataatc
     7801 ggttttttct ccgagaccgt aaatacaaga gacactagcc acaataatca cgtcacggcg
     7861 ggttagcaag gcttgagtag cagcatggcg caagcggtca atatcttcat taattgttgc
     7921 ttctttttca atataggtgt cggtgggtgg cagataagcc tcaggctggt aataatcata
     7981 ataagagaca aaatagtgga ccgcattttg cgggaaaaag gccttgaatt ctttatataa
     8041 ctgagcggcc agagttttat taggtgaaat aaccaaggtt ggcttctggg ttttttcaat
     8101 aactttagct atggtaaaag ttttaccact gccagtaacc cctaagagag tttgggcctt
     8161 gaggccctgt tgtattcctt ggcttaattt ttgaatcgct tgtggctggt cgccagtggg
     8221 ttggaattga gcttgaagtt gaaactgcat ggtagtactt agaattttgg taatagttat
     8281 attatagaga aaccataaaa aagtggcaat aaaaaaccgg cagtttatta aactaccggc
     8341 tatagttttt attttagtag tattaccttt ttagtcgctg aaaaacgacc tgaggtaatt
     8401 ttataaaaat aaataccagg acttactttt tgtcctgcat cgtctttact atcccaggtg
     8461 atagtatgtt ctcctacagc aaaaggttgt tgtgctagtg tgcgcactaa ttgtcccttg
     8521 acattataaa tggcaatggt agtctgctca ctagcctttt cctgcctgaa ttcaatattt
     8581 gtattatccc taaagggatt gggataacaa tagacactat taataatagg aggagtagta
     8641 gtatcatcaa ctggattagt gccagttgat atccccgaga gccgtattga aggtactgtg
     8701 gatagtaata ccagagtatt tccatgccac ctggcactta tataagtacc atttgtaccc
     8761 ggtggtagtt gggggctaaa agtccaggtg agttgtccat tcgcatcata attcattaaa
     8821 attggtacgg taccactact tgttgtgtgc gatccataga agagtatatt actaccattg
     8881 tttatcattc cttctggctg atactcctct gctgtaccaa tataatgttc ccaaaagatt
     8941 tcgccagtat ccaaatctag ctgctgcatc caccaggtag cttcattaag agtattaaag
     9001 tctatagtcg cagtgcagat atataatttg ttattgtttg ggataatata tctgcctact
     9061 ttatcccaaa tggtacattg ccaattaagc gagactccat taataaggcc attattagtg
     9121 gtaagagtct cttttctgat gttcccattg aaggtaccat cattaatcat cccacaaata
     9181 tagatattat agccatcggc gctataaata tctcctcccc attctggggc aatccaatca
     9241 tccccgagac ccaaattacc tataatatta ccggtggtgt tatccaaaca aacccacccc
     9301 atccagacat taaggtaggc tatatactcg gggaaggtat atatagtcca ccatggtgtt
     9361 tgctgatgca atgaaggaaa tatatcctgg gtccagatgg ttgagccatc ctgcgctgat
     9421 attttatagg caatatggcg ttcatcaacg gttaccagat tattatctga tgttacgcct
     9481 agttgttttt tatgcaccac ctcattacct ataaaacctg ctcttgtcca cccgatttca
     9541 ccggttgtat agtttacctg gattaaggtt tgtgggtagg gataaggccc acttaaatac
     9601 attaggaaga tgttatcccc aataatttcc atgttataac agtaaatccc tattatatct
     9661 agtggcatgg cccatagttg atgaggggtg gtttgagctc cgagggacgt gaaaaccatt
     9721 atcatcatga taataatggt taatgaaata ttgcgtatcc ttttcattgg acacctcctt
     9781 tattttttaa agtacaatat tttaattctt tcttacttta taatattata gacgaaacgt
     9841 caattatatt gtgctccctt tttaaataca ttatactcat taataatgaa gattattaaa
     9901 ctaacaaaat tttatcatca agcttttacc ttagtagaac ttctagtagt tattgctatt
     9961 attggcattc tttctactct ctcaattgta ggtttaaata gcgctcgagc taaggctcga
    10021 gatagtcgcc gtttatctga tgttcgagaa atagctaccg ccctagagat gtattatagt
    10081 gatacaggag catatccctt tgtagcttct gccactggta ctctaatagg tggtttatgt
    10141 ttaagtgatt taggcattag ttcaacttgt ggtaccagtg tatatttaaa aaaatccctg
    10201 acgatcctca gggtaggacc cattattggt atatgtctag tagcaggggg gcgagctacc
    10261 agattaattt tcgcttagag caaggctctc aaggttatcc ggctacttct ttggttatgg
    10321 ttcctggtgg aattcaagag agtctttatt atggtttaat tggctggtgg ccctttaatg
    10381 aggggtctgg gctgcttact agtgatgcta gtggccataa taatcaaggt ataattagtg
    10441 gtgccttatg gcaacctcat gagccggggt ggtcactctt atttgataat gttgacgatg
    10501 gtgttaatat tcctactaat aattttgtag gactatatga atatactatg tccgcctggg
    10561 ttaaacctgt aggtacacat aaaaattata ctggtactat tatttcttct ggcaattgga
    10621 attctattca ttgggctttt ggcttggggc aaaataatga atatataaaa tttaatcgag
    10681 ccgatggctc tcgttgggtt agttttagtt ataatgtccc cctaaatcag tggacccatg
    10741 tagcggttac tagatttaat gataaattaa actattttgt aaatggccaa tatattgggc
    10801 agtctagcat tagtgcttct ggtgaattaa attctaatgc tactaatact atgatcggac
    10861 gggagaccta tgctaatggt tattttgatt ttaacggagc tattgatgat gtccggattt
    10921 ataatcgtgc tctttctagt gaggaaattg ctattttagc tcagcaaaat taggctattt
    10981 ttgacagggc tagttttttt tcttaaaatg taaatattaa ataatttaag gaaatagttt
    11041 tatggaccag ccgtcctcct tttttgcgca aagtgaattg gaagttttgg ctttttggga
    11101 tagagctaaa atttttgagc agtctgtaga aaagacagcg cttcatggtg attatcgttt
    11161 ttatgatggg ccgccctttg ctaccgggac cccacattat ggtcatattg ttgccagtat
    11221 tatgaaagat gtggtacctc gtttttggac catgaaaggt tatcgcgtag aacgtcgctg
    11281 gggctgggat tgtcatggct tgccgattga aaatattgtg gaaaaagaat tgggttatcg
    11341 acacaagaaa gatattgagg ctttaggtat agataagttt aatgaactgt gtcggtctaa
    11401 agttttaggt tatgtgaatg aatggcgcac cattatccat cgattgggtc gttttgtgga
    11461 tatggataat gcttatttga cgatggattt atcgtttatg gagtcagttt ggtgggtgtt
    11521 taaagaatta tgggataggg gattaattta tcatggatat cggtcaatgt atatttgtcc
    11581 gcgctgtgaa acaacattgt cgcaatcgga agtggcagag ggttacaaag atgctaaaga
    11641 tttatcctgt acggttaaat ttaagttaca gcctaatcaa aagatcggtg attttgtcat
    11701 taatgattca acctatattt tggcctggac cactaccccc tggacgttaa tggctaatgt
    11761 ggccctagca gtaggtgcgg acatggatta tattgtggta gagattccag ctaattttga
    11821 ctttaagcaa tttaatgttg atttagattt aactgggaga tatatttttt ctcctcagtg
    11881 tgcggcccaa aacgataagc ttcatcagct attagaatta gctctacaag ggcacgttcc
    11941 aagttataaa tttaaggggc acgatttagt tggtttaaag tatttaccgc cttttgattt
    12001 ttattattcc cagtctgatt tagcaaataa agataagggc tggcaagttt atccggctga
    12061 ttttgtcaac actgaagatg gtacaggcat tgtccatatt gcccctggtt ttggagaaga
    12121 tgattggcag ctagcaaaac aatatgattt gccttttgta cagcatgtta aaatggatgg
    12181 cacaattaaa gatgaagcag gtgaatgggc tggcttaaat gttaagccta aggaggatta
    12241 ccaggcaact gataaaaagg ttgttgaatt tctaggaaaa agtggcttgc ttttttctca
    12301 agaggaatat gaacatagct atccatattg ttggcgttgt gatactcctt taattaatta
    12361 tgctacttcc tcatggtttg ttaatgtgac taaaattaag cctctcgtgc tggaggaagc
    12421 caagaaaatt aactggtcac ccagttatat taaagaagga cgttttggac aatggctgga
    12481 aggagccaag gattggtcta ttagtcggca acgctattgg gcctctgtta tccctatttg
    12541 gcaatgttcc tgtggctggc agaaagttat tggttcggtg caagaattag cagagcttag
    12601 cggaaaagag ataactgatt tacataaaca tattgttgac aaaattacca ttaagtgtcc
    12661 taattgtggg aaattggttc atcgagtacc tgatgtacta gatacttggt ttgatagtgg
    12721 ctctatgccc tatgctcagg ctcattatcc cttcgaaaat gctgagaatt ttgatagcca
    12781 ttttccggct caatttattg ctgaaggcat tgatcagacc cgatgctggt tttattatct
    12841 tcatgtagtt agcacggctt tagggcgggg agtggctttt cgcaacgtta tcgtcaatgg
    12901 tattgtgctg tctgaagatg gcaagaagat gtccaaaaaa ctacataatt atcctgatcc
    12961 agctgcacta atggataaat atggtgctga tgccttgcgt tactatttat tagcttcacc
    13021 agttactcaa gcagaaaatc ttaattttag tgagactgga gtggaagaag cagttaagaa
    13081 aattattctc ccggtgtgga atgtgtataa attttacgat ttatatggag gaaatgaacg
    13141 ctctgttttg tcaagagaag attggcattc agacaatatt ttagaccgct ggattgtggc
    13201 acgtcttcat cagttgctgg gagatataga tagtgccatg atggattata atttacctaa
    13261 ggcggtacgg ccactggcta tcttcattga cgacctatct agttggtatt tacgccgttc
    13321 tcgcaatcgg tttaaaagcg gggagtcaca agataaagct gcggccattc ttactactag
    13381 ttatgtgctt cagcatctag ctatagcctt agcgccattt actccctttt taggtgaggc
    13441 cttgtggcaa aaaactactg gttttaatta tgagcatgat gataaatcag tgcatttgct
    13501 gaattggcca accgatttgc cggtagatga aagggtttta acagaaatgg cagtggtgcg
    13561 agagattgct gaattagctt tagctgagcg cgatgaagca ggattaaaga ttagacaagt
    13621 attaccaagt ttaaccatta aaggaccaac ggatcgctta tcactgttga cttcagccag
    13681 tctagatatt ttagctgatg agcttaatgt taagcatatt gttactgaga ctaataattc
    13741 tgccggctgg gaggtaaagt tggatactac tttaacgcct gaactgatac gggagggttt
    13801 agctcgagaa ttggtgcggc atattaactt acttcgaaag gagttgggtc ttaccttgtc
    13861 aaaccgaaca attatttatt ggtctagtac tagcccacta gccaatttaa ctctagagga
    13921 atgggccgcg actattcaaa aagagacact tagtgctaaa ctagaagaag gccagcctga
    13981 ccaatttttg gcccacaaga ccctcaactt agatgatggg acactagaaa ttatgattgc
    14041 taaattaaat taaatattaa taaaaacaat tttatgatgt tagttcttta cattttatta
    14101 ataattattg ccatttttat tatctggctc attgccgtct ataacggcct tattgctagc
    14161 cggaatcgtt tagctgaggc tttaagtgat attgatgttc agttaaagcg acgttatgac
    14221 cttattccta atttagtgga gacggttaag ggttatgctg cttatgagca ggaaactctt
    14281 aataaaatta tcgaggctcg taatgaggct atgtcagctc ataaaactgg cgacaaggaa
    14341 ggagaaaaag cagcggaaga taatttgtca ggcttactca aaaatttatt tgctttaagt
    14401 gaaaattatc ctgatcttaa agctaaccaa aattttttgg agctccagcg tgaacttacc
    14461 gatactgaag acaagattca agctgccagg cgtttttata atagcaacgc ccgtgatttt
    14521 aacattaaat tacaggtatt tcctactaat cttgttgcga aaaaattagg ttttacgcca
    14581 gtagaatttt ttgaaattac tgataaggca gaaaaagaac aagttaaagt tgaattttaa
    14641 aaatatgaat ctatatactc atattagcgc caataaacgt cggagttggt tgctaattat
    14701 cattaatgga gtattgcttt tagctttagg ctgggtcctt agccaagcta ctaatgatta
    14761 tatttggttc tatattgcgg taatttattc tttggtgtct agcttagttg gttatttatg
    14821 gggtgacaag gtggtattaa tgactagcaa agctaaatta gttgaccgta aggatgcacc
    14881 tgagctttat cgcttggtag aaaatttatg tattgctgcc ggtttgccaa ctcccaagat
    14941 ttacattatt aatgaagctg ctcccaacgc tttcgccacg ggccgagatc caaaacatgc
    15001 ttctgtggct gttacttcag gactgttagc taaattagac aaagctgagc ttgagggggt
    15061 tatcgctcat gagctttctc atgttggcaa tcgtgatatt ctattagcta ctttagtgtc
    15121 agtagtagtg ggggcagtag cgctcttggg taattggttt gcccggtggt cattttgggg
    15181 tggcaatcga cgctctagta ataatgatga ggcaggacaa atatcggtta tcttgtctat
    15241 tattgcttta gtattagcta ttttagcgcc tattgtggct cagttaattc aactagctat
    15301 ttcgcgtcgc cgagaatttc tagctgatgc cgatggtgtt cttttaactc gttatcctga
    15361 aggtcttatt aatgccttaa agaagatcag tgctgatcct caattgcttt cagtggccag
    15421 tgaagcaact gctcatttat attttgcttc accttttgat aataagaaac ggacaccgtt
    15481 actagctaaa atgtttatga ctcatccgcc ggtggaagag cggattgctg ctttagaaaa
    15541 aataaatatt taatatggct aatcctgact tacccaaaga ccttagtaag actgagaata
    15601 tagcggctag tggccttaaa gaagtatggc gtgctgaaga acaagagcac cgacagcgta
    15661 aaattttttt aattatcggt attttattag taattatagt cattggtggt ttaggtttta
    15721 tgagttggcc ctatgttcag gggcaaattt atggttatta tgataaaata agtaaggaga
    15781 taaaaagttg gccggctaaa tatgcagctt ggcagaaaaa aaatgaggag gcagttatta
    15841 agggtgatgt ttttaaataa gacatgaaat gccaataata tgcttgcttt tattaaggga
    15901 gagattaaag ctaaacgtct agggtcaatt attgtagaaa ataataatct cggctaccag
    15961 attatggttg gcgagaaaat ttgggaagaa gctaagacag gggaaacaat tgaattattt
    16021 cttcatcatc atttaagaga ggatagtgat gaactttatg gttttgctga tttggagacc
    16081 ctgtctttat ttgaacttct tctttcagtg tcaggtattg gtccgaagtc ggctttaaat
    16141 attttgtcgc ttgccgatat taaagacgtc cagcaggcta ttattttagg tgacgaagtg
    16201 atgcttacta aagtatctgg aattggccgg aagacagcag agagagtgat tttagagtta
    16261 aaaaataaaa ttgctagttt accagctaat ctcttggcag gcgaagaggg tagtagtaat
    16321 gaggatgatc tggaagcctt aattgctttg ggttatagcg caccagccgc ccggcgagct
    16381 ctagctaaag tagatcgaaa aattactgac ccagctgaac gtttgaaatt agccttaaag
    16441 gaattgcctt agtttatgtg gcagatcata gattctctgg aagccgatga ttttgtaaaa
    16501 cactatgggg catctgaatt tttacaaagt tcagcctggt tagatattca acgtcaattt
    16561 tatagcgtag agactttagg ctattattta gataaggagt taatgtctgt ggctcgtctt
    16621 attcgacgac cgctaggagg aagattttgt tattattatt ctccccgtgg tcccattgtg
    16681 agaaagactc attataatct atggccacaa cttattaagg atttagggcc atggcttaag
    16741 gagagacggg ctttattttg gcgcctggag ccagggagta actggttaga ttatgatttt
    16801 gcctcattag gtttttatca tgggccagat attcagccga gtgagacacg tgttcttagc
    16861 cttgattctt ctcccgagga attattttct aggatgcatc ccaagacacg ttacaatatt
    16921 cgtttagcta gtaaacgggg agtaactgta cgtagggctg gtccccaaga ttttgctagc
    16981 ttatggtctt tattacttct taccagtcag cgtgataatt ttcatttaca ccgccgggaa
    17041 tattatttag ccctaattaa ttcaccagct attgaattat tgttagctga atatcatgga
    17101 caggttttag cagctggcat ttttgcttat tggggagata cagctattta tcttcatggg
    17161 gcctcgggca atgaacatcg tgagcttatg gctccttatt tagtgcagtg gcaggcaatt
    17221 caattagctt atgaattagg tgctcggcgg tatgatttat ttggtacgag ttctcaaaaa
    17281 tggccaggga ttactcgttt taagcagggg tttggcgggg aaatagttca ttatcccgga
    17341 acttttgatt ggccactttc taaatggaat tataatattt atgctactct tcggcagtgg
    17401 cgacggaaaa aacattaata atctttatga ataaattttt tgctcaatta aaaaaaacta
    17461 tttacaatag ttatttaaag catttagtac cagcttatca ttttatagtt aattggctag
    17521 ctgatgttta ttataatcat ccaagtcggc agttaattgt tattgggatc actggcacta
    17581 ctggcaagac cactagtgtt ttactgaccg ccgaggtact acgggccgct ggctataaag
    17641 taggctatac ttccacggca atgtttagtg atggtcagcg tgattggctt aatgatacaa
    17701 agatgactat ggtagggcga ttctttactc aaaagatgtt acggcagatg gtcaataatg
    17761 ggtgtcatta tgccattatt gaaacgactt ctcagggaat attacaatat cgccatcgtt
    17821 ttattaatta tgacttaact atttttaccg gtctttatcc tgagcacatt gaggcccatg
    17881 gttcttttga taattatcgt cgagccaagg gccgtttgtt ttctcacctg cgtcgttgtg
    17941 gttataaata tgtgggaagc gacaaacatg ttgaaaaagt tgatcatggc tttaaaaaag
    18001 tagggctgga taaggtgcct aaaactattg ttgccaatct agacgatgat tatgctcatt
    18061 attttttaag ttttggcgcg gataaggcct ggggctatac cactaattat aaagagaaaa
    18121 aggatttaga atggcctgat tatcttatta tgctagaata tggccagata aagtcagata
    18181 tccatggcac tgattttatt tgcgagggtc aagaaattca tctgccgctt ctgggggctt
    18241 ttaatgccag taatgcgatg aatgctattg gagtagcctt aagccagggg atagattggc
    18301 cagtgattaa agccggatta ggtgcagtga aggtattagc tggccggcta gaaattattg
    18361 atgaaggcca gccatttagc gtgattgttg attatgcttt tga
//
LOCUS       DAOOIS010000009        17137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_3242_length_17137_cov_40.143953, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000009 DAOOIS010000000
VERSION     DAOOIS010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17137
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3242_length_17137_cov_40.143953"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PK960_01665"
     CDS             <1..1137
                     /locus_tag="PK960_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07913.1"
                     /translation="RPQSFPAPSLEFLCKALCPGCYDPSGNNGNGSCSNSNNEVLLGS
                     EIYRDQFSIVVTNQSSSVPAPTSSVYCTKFVKVVDEFGNNIFWSDRVKEGSSFQMPCY
                     IPNASSSTGFKQVSCAFSSPSIPFASIAYPAGLGNYPDLWDGAVGNNNTGNEPLFYQD
                     AASGVHLGRIFSEDQNNLAHPRYLFAKSYSFYEWKFDQNAGLNGVHGYTGDSGHYNIS
                     TSSYGYSWTPPTTLCSGGIRPSSTSSFSYCLVKPRINNVKINGKSADSGSIITFSEPV
                     FTAELTFNSIIDKDQKPLSQITINWGDGSEPYNRSGSFLDHPDPASPHRYTHRYICQE
                     DAATGGCRINNLSITIQDHWGGTSTAYSINILPNASQSSGGVNY"
     gene            1194..1877
                     /locus_tag="PK960_01670"
     CDS             1194..1877
                     /locus_tag="PK960_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07914.1"
                     /translation="MAEAALVLDNNSQDQAVADLLRQQKNQERLQQSNAEMVDSQVNN
                     SNINDRLASRVAPGSQEETASKSSSNKKADAQETAEALKKAKQLAKLAANPTVAGVAK
                     MAAQQTAQAVKQGELGKKLTGELLKQSWTHLVDSFGLTLIYLNIHAWVGFLEGHKIFC
                     KLGEETKIPGKLKSSMGMLEIMGIIALDFLVFLIILGILAIINVIVDALVHPTRIFGA
                     LWDLLGISF"
     gene            1884..>3308
                     /locus_tag="PK960_01675"
     CDS             1884..>3308
                     /locus_tag="PK960_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07915.1"
                     /translation="MAVWWSKISGYFMERWCRLSKTQKCLIIVLCLLIILIPFIASAD
                     FADTITKGVVQILTAIVSVLVSFVGKVLALVLHCLVYVAQYNNFINETVVKEGWKVVR
                     DVCNMFFVLGLLLIAFGTVLRLPNYQAKNSLKNFVLGAVLVNFSKTICGLILDFAQII
                     MLTFVNGFKDVGVGNLTEMLGLTSLFDVVVNEAEVTAIQSVGIFVSYLMGLIYLIVAL
                     VVILTMTIMLIQRAVMLWLFIVLSPFPYLLSSFPQSGKMGSLGKEWWNRFSGLVITGP
                     LLAFFIWLSFFTASSPTTTTSTNNKEEIQLTGLTAEVEAPTEGDYFGDLNAGVTKSTT
                     AQAIIKFLVSIGILITGMTLTKEASSMVGNVVGAGMGALQKTGKGAVNFAKKETTKAA
                     TNTAKWAGRNALKGAGGLTTTLTKNSTHGIGKTLNKVGTFAKTWGEDLTESRHKEKVD
                     KRLKTLKKMGMGEKSMGALKEVTDD"
     assembly_gap    3309..3408
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3409..4551
                     /locus_tag="PK960_01680"
     CDS             <3409..4551
                     /locus_tag="PK960_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07916.1"
                     /translation="FAKGPARKLDRDMENSRIMAHEKVNKDYHNAVQGAADKRSEDLT
                     NLARAKDEGKITKDEYNKQKNKIEEDFQKAKEEARQAMIVEQKRISHPEIDQKFKEIE
                     QEKSEKTKEIETAHAQRVEDAANERNKIIDDNTRNKDEAQKQFADGAISKTEMERLLL
                     KADLNIKQANADYDKKVEESQKIKETESKQVEKDYQEKRQTIIQDIKDVPVGDSMERL
                     MANFSVGSFVSNLAKSVADYKPMSTTSEALQKGVKEKQEAKDNIAAISSMQNPGDLAK
                     LLDPQLFSAGIGNAIKNAQREFYALLSNGQRSSQQAIKNLINAIQRLDANNEKDKVLL
                     LSIKNGVQTYQRQGGDPAGRLDPLLKEASNSLKKVSPSEGSSESKK"
     gene            4564..5544
                     /locus_tag="PK960_01685"
     CDS             4564..5544
                     /locus_tag="PK960_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07917.1"
                     /translation="MKNIYDQETLEAYLVEFLTMPPIVVAEFNRPSNYKILTDLAAEY
                     KVNLDIVVLLVAINRVSMDELDQYLVDNLDLDRAKASELATKMRDNFFLPIINKIAFL
                     SSQPSPEDYEIIENIFAKQLLNEINADPILIKALNQKIFAWLSEDLDGAKRRIENAML
                     KNQEIVTKEKITMNDDKLPGTIGNWLHCFTAEKGAANFDSLSISEFLSLSPNTTHLTV
                     EEKKKLSIVLNTYRNIKFFPDSMPSDDGSGWAIIPLPVVEPSSQASKPKEEGSPEVSS
                     SKSDIESPQLSSDSDRENKINQLQLLLKRYGTDTIEGQAIMEEIAKLKGS"
     gene            5550..6833
                     /locus_tag="PK960_01690"
     CDS             5550..6833
                     /locus_tag="PK960_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07918.1"
                     /translation="MFDYLQKYQQIPAEIRAKLDNASVKANIHQLEQQYRVSLFNIVM
                     KVMVRDIPLDRLSFYLAENEGMNKEQAQKLVADLQANVFSLVQDYLSKPLYEPINIDQ
                     LVQHNRDISPSASTEPHNINFSETLEVKLNEFVSHQKIVKKSAELNRLRQIAKTYLLG
                     VRNQQAFVDIATKTVAEGGLGLSADKALSLVAELNNIKQNLEFEARQQVNIQPPAKPI
                     DKLIKQSIVEDFESELLANLRQIDKQPRYDLKTEYELTAGTKPETKLLTSTTEAEQEI
                     VKQPTVKEAAVNQPAAVVVPLDQRVTDAKTGKIRMDDVKFIPKTLTPIDELATISLKK
                     FRYLGPTAAERTKKIKEKIDLLTEYGYSKRLQGINAWRHSPLNLLYLKIGQLSIEKGV
                     SAEEILTKGAPELGEDILTLDEFKAILELNKQLRF"
     gene            6840..7307
                     /locus_tag="PK960_01695"
     CDS             6840..7307
                     /locus_tag="PK960_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HPD07919.1"
                     /translation="MQQFTVPQFIDEEDKILGPVTTRQFIILLVAAGFMFVEYKIFDF
                     SLFLVSALITAGAFMALAFMKINGRPFHFFLLNLIQSLFSPKLRVWNKNFGKVENIIE
                     FDYKNIIKTQPPAAKVRFSSSRLAELSLIVDTFGAYRGEAQEPEELANNKIEL"
     gene            7304..7981
                     /locus_tag="PK960_01700"
     CDS             7304..7981
                     /locus_tag="PK960_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HPD07920.1"
                     /translation="MNSKRSNTLTSTQQYLKIADIKQDTLVMKDGTLRAVLMVSSVNF
                     ALKSEEEQEAIINGYISFLNNIEFLLQIVVQSRELNINGYLEMMRKRANEQTNELLKN
                     QTLQYIDYVSQLVSLGKIMSKHFYVVVPYSPISDKRKNFWQSLLEAFRPIDVIHLKEE
                     RFMLLKKQLDSRVDNVASGLLSMGLNVVRLDTQGLIELFYNSYNQKTAANQPLDNIDK
                     LNINNSF"
     gene            7984..9867
                     /locus_tag="PK960_01705"
     CDS             7984..9867
                     /locus_tag="PK960_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HPD07921.1"
                     /translation="MFNLNSKKKLSPDTHDRPSLGNQNLQFLSEQAALAEELIAQEKA
                     YRYGVVNIRDLIAPASLEVKPKYLLLNGQYVSTMFVVTYPRYINVGWFAPILNLNESF
                     DIAMFFYPIKSSIVLKQLKNKVGVLEAQISADIEKGNPRDPIRETALRDVEALRDDLT
                     QGIEKFFQFALYVTFYAETEEKLEQLANQLEDLFGSRLVYSKRVFYQAEQGFNSTLPM
                     CSDELNIAYNMNSSPVASSFPFISSELTSDNGILYGINRHNNSLILFDRFSLPNANNV
                     VFATSGAGKSYAIKLEVLRTLMMGTDVIVIDPEYEYKALTEAVGGTYINISLASEAKI
                     NPFDLPRPVGQETKTEDIIRSAVITVKGLIRIMLGNLTTEEDSIIDMALLETYAKKDI
                     TPDSDLSTIEPPTMKDFQYILEGMEGGEGLALRLKKYTEGTFSSLFNSPTNVEMNNQL
                     VAFSVRDLEDELRPLAIYTIVNYIWNIVRSELKRRILVIDEAWWLMQYEDSAKFIYAL
                     VKRCRKYYLGVTTITQDVNDFLLSPYGKAIVTNSAMQILLKQSPAAIDLVQKTFMLTE
                     GEKYLLLESGVGEGIFFAGNRHAAIKIVASYLEDQLITSNPEQLLKMEKEAQQNQEKI
                     MNK"
     gene            9883..10515
                     /locus_tag="PK960_01710"
     CDS             9883..10515
                     /locus_tag="PK960_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07922.1"
                     /translation="MKISSKMRNWLLLGIVVIILIILLYLLLSKKLEVQAPQLNEQPI
                     VEEFSTPTETPLAILETDKVASNNQVPLDDRARTEVQLEKEASAFVERWVSLSNQDNF
                     SNLAILKNQMTDRMINWTNKYIQENMPSSSNNKLYMGVEGQAIVANGNLKSETATSLT
                     VEVKIRGKETIASTVNQPKIFYQDYQVDMVKSGGVWLVDKLTWGERQYQE"
     gene            10580..11446
                     /locus_tag="PK960_01715"
     CDS             10580..11446
                     /locus_tag="PK960_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD07923.1"
                     /translation="MLKPKSKLILSLTLLCFILLGCFTVRYYYRDNHYLKIVFLNIGQ
                     GDATLITTATKKHILIDCGPDQAILNALDQELSWWDRTIDIVIISHDHADHWGGLSDL
                     VKKYKIEQVILAKPSQISSEFASVLNNISTQNIKITNLYAPAIVNLDDGSKLSIIWPT
                     EANIAEQDNINNQSLVIKWQYGQKFFLWTGDLESDAEDELMANYTVNEPINILKIAHH
                     GSWTATSPRWLNYWQPDLAVISVGANNSLGLPNEIILDRLKRQGVQIWRTDINGKLVI
                     YTDGQTLWRANK"
     gene            11505..11999
                     /gene="ndk"
                     /locus_tag="PK960_01720"
     CDS             11505..11999
                     /gene="ndk"
                     /locus_tag="PK960_01720"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPD07924.1"
                     /translation="MAKKRQERTLVIIKPDALNRSLAGEIIHRFERKGLKIVGLKMIQ
                     LDDIILAEHYAHLADQPFFPRIAKFMSNWPVIVMALEGLEAVNSVRLLTGETLGRHAD
                     AGTIRGDYSMSVQANIVHASDSVENARKELARFFRPDELFDWDRLDSQAIYAEGEGEE
                     DDDE"
     gene            12011..12084
                     /locus_tag="PK960_01725"
     tRNA            12011..12084
                     /locus_tag="PK960_01725"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12043..12045,aa:Pro,seq:ggg)
     gene            12110..>13727
                     /locus_tag="PK960_01730"
     CDS             12110..>13727
                     /locus_tag="PK960_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07925.1"
                     /translation="MKEKFTEANRFLAEKEAEVIENIECPEDKEDFEGQKVLIIGGPD
                     AHGVSMAVSSALYLEKRGAEPTIYVGSPVIREGRGATHPGAFYCKTIPNLNVANYDRL
                     IIVDIPLDFRDLSGSEKAIIELDERLRQDRQQRGQSPLKHSTFYLDHHSTTQFTAPHD
                     SILIKNVPVAEACKLGNDKTLIGRIGAICDRDPATLPITSEEMMLARGLDAAVRPDPD
                     DERPSLPKNASEEEKQNYQKQLREWEDRAQQRLEEAVKRLKAEDWSYFKKEAERINQI
                     EIPTSSGFGQIAIVDTQNLTSKLGVLKLMEMAIENMGIDETPYAIGVLRELGDIKMGR
                     EPADVITVIRHWTREDLPSVEEVITHYFGEEFIEKYKIYGAKNAKTLRLPVNDQETAK
                     LTAQLVRAFAGREMPDFSQVRSVVMCGDPNSGKSVYSTIFREALKNLGIRVAHLDLDK
                     AAPTPQWYLEAEIASKEAELLFSQGKLSPEKLAEARKKLDEAAEKRRAMKRPWSLELA
                     EEAKQELLEAARNENNDFVIGDIGGGKIKKD"
     assembly_gap    13728..13827
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13828..14250
                     /locus_tag="PK960_01735"
     CDS             <13828..14250
                     /locus_tag="PK960_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07926.1"
                     /translation="ENNDFVIGDIGGGKIKKDSEGNIVKITRLTAENAKILEGADAVI
                     IVSNNPQGAAEWKKLIETGIDPETGVEIKRDKPIQILGIYQSTLEGNVQKIAGDKEIG
                     IITNLDRSKAEKKYNPAIFTTAIFVSEAVQKRRQNMND"
     gene            14271..16190
                     /locus_tag="PK960_01740"
     CDS             14271..16190
                     /locus_tag="PK960_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPD07927.1"
                     /translation="MLLEKIDALSVDKQPKSEKIEDKIGNKELPIWDKMPEIVETLKT
                     NNRLILVAETGSGKTTQVPQALWAEGFAEQGTIFVVENRVAVAVEIAKRVAEEMKVKI
                     GQEVGYLTGPEKKSSRKAQIIFLTSGVFKNIIREDPKMEQASVVLFDEFDERYLLSDI
                     GLALVEKAQDQGSMVKFGLMSATLNAQKFSEHFGGAPIVEAKGRPYPVESHYAADSIS
                     LYRMPEKSAELALSIHQSSSHGDILIFMPGKQEIKATIDEMLSRNITGATILPLYSEL
                     SPQERHRVFEKQSGRKIIVATNIAERGLTIDGIRYVIDSGLVRMNEYDPAADTTKLMT
                     VPTAQDSLRQRQGRAGRTQPGECYFLITEDEFNHRPTTTKPEIMRTSLREVVLQIKAM
                     GYSREEDSIRLIDSPDKKNWQIAKNQLRLLGALDPQNETKLSEFGYKLSELSCDPREG
                     TMLLRGCEMGCGQEMALIAAIRTSKRLLYRPQDEIEQADAAHKQFEISNLSDLLNLAN
                     VYEQAKRMNFDSRWCRKNYVSWTALREVQQNYLQLLSQIKKLGYQINQDKAKPEIICR
                     AMATGFPDKIFENIGRGWYRNTTTGEEALLGRESCVSGNLIIANELITIQTRRGGELP
                     LITLATKLEPTWLNN"
     gene            16212..17084
                     /locus_tag="PK960_01745"
     CDS             16212..17084
                     /locus_tag="PK960_01745"
                     /inference="COORDINATES: protein motif:HMM:NF021159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx3"
                     /protein_id="HPD07928.1"
                     /translation="MNEAKFTPEAYTSQETKEHEPKIERLSINDFAREIGLEPDAQGR
                     ITFGPEHVRLAYEYAEKLARDKHAEAIVIDGVASPAVIASLLHGAHPAEGYLTYMQKD
                     PEGTMVKNEIKVLEPLPQGDGSGPEGFTWFKKEAPDYTLVEFALSGDFKADDLAKVIP
                     PEVDPTKPVLISGRGPLYLTQTIAAAYRHYKGVPGVGFYQPASKFGPAKTEIGISHNE
                     DLPLGLNFGEPSEIGSAKEQLEKGINENLAQIKEGIEKGQISSVEVQGKTIKITLENG
                     TTYNISVREVIKNE"
BASE COUNT         5579 a         2678 c         3503 g         5177 t
ORIGIN      
        1 cgtcctcagt cttttccagc accatcatta gaatttttat gtaaagcttt atgtcctgga
       61 tgttatgatc cttctggtaa taatggtaat ggaagttgtt cgaatagtaa caatgaggtg
      121 cttttaggta gtgagattta tcgtgaccaa tttagtatcg tggtaactaa tcagagttct
      181 agcgtgcctg cccctacctc ttctgtctat tgtactaaat tcgttaaggt ggttgatgaa
      241 tttggcaata atatcttttg gtcagatcga gtcaaagaag gcagtagctt tcagatgcct
      301 tgttatatac ccaatgctag cagtagtact ggttttaaac aagtctcatg tgccttttct
      361 agcccttcaa taccctttgc ctccattgct tatccagctg gtttaggtaa ttatcctgat
      421 ttatgggatg gggcagtggg taataataat acgggtaatg agccgctgtt ttatcaagat
      481 gctgcctctg gggtgcattt agggcgaatt tttagtgaag atcagaataa tttagctcat
      541 cctagatatt tatttgctaa atcttatagc ttctatgaat ggaagtttga tcaaaatgcg
      601 ggccttaatg gagttcatgg ttatacggga gacagtggac actataatat tagtacgagt
      661 agttacggtt actcgtggac gcccccaact acactctgta gcggtgggat aaggcctagt
      721 agtacttctt ctttctctta ttgtttagta aaacctcgga ttaacaatgt taagattaat
      781 ggtaagtcag ctgacagtgg ttcaataatt actttttctg aaccagtttt tacggcagaa
      841 ttaactttta atagtattat tgataaagac caaaagccac ttagccaaat tacaataaat
      901 tggggtgatg gctctgagcc atataatcga agtggtagtt ttctggatca tcctgatcct
      961 gcctcacccc atcgttatac ccaccgctat atttgtcagg aagacgcggc tactggtggc
     1021 tgtcgaatta ataatttgtc tattacgatt caagaccatt ggggaggaac aagtacggct
     1081 tattctatta acattttacc aaatgcttct cagtcttcag ggggtgttaa ttattagcta
     1141 gtcatttgct cgcctgatag atttatgtta taatttttga tgtattcaaa attatggctg
     1201 aggcggcatt agttttagat aataattcac aagaccaagc ggttgctgat ttattgcgtc
     1261 aacaaaaaaa tcaagaacgt ttgcagcaat ctaatgctga gatggttgat tctcaagtaa
     1321 ataacagcaa tatcaatgac cgtttggcgt cacgggtagc accaggtagt caagaggaaa
     1381 cagcctcaaa gtcgtctagc aataaaaagg ctgatgctca agaaactgcc gaggccctta
     1441 aaaaagctaa acagttagct aaattagctg ctaatcctac tgttgccggg gtagccaaaa
     1501 tggccgctca acaaacagct caagcagtaa aacaaggtga gcttggtaaa aagcttacag
     1561 gcgagctttt aaaacaaagc tggactcatc tagtagattc ttttggtttg actttaattt
     1621 atttaaatat ccatgcttgg gttggtttcc tagagggcca taaaattttt tgtaaactgg
     1681 gtgaagaaac caaaatacct ggtaaactta aaagctctat ggggatgcta gagattatgg
     1741 gtattatagc cttagatttt ctagtatttt taattatttt aggtattcta gctattatta
     1801 atgtgatagt tgatgcttta gttcatccta ctagaatttt tggtgcatta tgggatttat
     1861 tgggtataag tttttaataa tatatggctg tgtggtggtc taaaataagt ggatatttca
     1921 tggaaagatg gtgcagatta agcaaaactc aaaaatgctt aataattgtt ttatgtttgc
     1981 tcattatttt aattcccttt atagcttctg ctgattttgc tgatacaata accaagggcg
     2041 tagtgcagat tttaaccgct attgttagtg tactggtttc ttttgttggg aaagtcttgg
     2101 ctttagtgct tcattgttta gtatatgtag cgcagtataa taattttatt aatgagacag
     2161 tagttaaaga aggctggaag gtagtgcgtg atgtttgtaa tatgtttttt gtcttgggtt
     2221 tacttcttat tgccttcggg acagttttaa gattaccgaa ttatcaagcc aaaaatagcc
     2281 ttaagaattt tgtccttggt gctgttttgg ttaatttttc taaaactatt tgcggtttaa
     2341 ttctggattt tgcccagatt attatgctta cttttgtgaa cggctttaag gatgtaggtg
     2401 ttggtaattt aactgaaatg ttaggattaa cttctttatt tgatgtagtt gtcaacgaag
     2461 ctgaagttac agcaatacag agcgtgggga tttttgttag ttaccttatg ggactaattt
     2521 atttaattgt tgctctggta gttattttga ccatgactat tatgctaatt caacgagcag
     2581 taatgttatg gttgtttatt gttttgtcac catttcctta tttattatcc tcttttcctc
     2641 aatcagggaa aatgggtagc cttggtaaag aatggtggaa tcgctttagt ggattagtaa
     2701 taactgggcc acttctagct ttttttattt ggttatcatt ttttactgct tctagtccaa
     2761 ctacaactac tagtaccaat aataaagaag agattcagct aactggttta acggccgaag
     2821 tggaagcacc cactgaaggt gattattttg gtgatcttaa tgctggtgtt actaagtcta
     2881 ccaccgctca agctattatt aaatttttag tatctatagg tatacttatt acgggtatga
     2941 ccttgaccaa ggaagctagc tctatggttg gtaacgtggt tggagctggt atgggggcct
     3001 tacaaaagac gggtaagggg gcagttaatt ttgctaagaa agaaacgacc aaggcagcga
     3061 caaacactgc taaatgggca ggccgtaatg ccctaaaagg tgctggcggg ctgacaacca
     3121 ccttaactaa aaattctact catggtatag gtaagactct taataaggtt ggtacttttg
     3181 ctaaaacctg gggcgaagat ttaaccgaaa gtcgtcataa agagaaagtg gataagagac
     3241 ttaagactct taaaaaaatg ggtatgggag aaaaaagtat gggcgccctc aaggaggtaa
     3301 cggacgatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt tgccaaagga
     3421 ccggcccgca aactagatag ggacatggag aatagtcgaa ttatggctca tgaaaaagtt
     3481 aacaaagact atcataatgc agtccaggga gctgccgaca aacgttctga ggatttaacg
     3541 aatttagctc gggctaaaga cgaggggaag attactaagg atgagtataa taaacaaaag
     3601 aataaaatag aagaagattt tcaaaaagct aaagaagaag ctaggcaagc tatgattgta
     3661 gagcagaaaa ggattagcca ccccgagata gatcaaaaat ttaaagaaat tgaacaagaa
     3721 aaatcggaaa agaccaagga aatagagacg gcacatgccc aaagagtaga ggacgcagcc
     3781 aatgagagaa ataaaatcat cgatgataat acacgcaaca aagatgaagc gcagaaacaa
     3841 tttgctgacg gcgcaattag taaaaccgaa atggaacgat tattattaaa agctgattta
     3901 aatattaaac aagctaacgc tgactatgat aaaaaggtag aagagtcaca aaaaattaag
     3961 gaaactgaaa gtaagcaagt agaaaaggat tatcaagaaa aacggcaaac aattattcaa
     4021 gatattaaag atgtgccagt tggtgattct atggaaagac tgatggccaa tttttctgtt
     4081 ggcagtttcg tttctaattt agctaaaagt gttgctgatt ataaaccgat gtctactacg
     4141 agcgaggctc ttcagaaagg tgttaaagag aaacaagaag cgaaagataa tattgccgct
     4201 ataagtagca tgcaaaatcc cggggattta gctaagctcc ttgatcccca actcttttct
     4261 gctggcattg gcaatgctat caagaatgcc cagcgcgaat tctatgctct gttaagcaat
     4321 gggcagcgca gttctcaaca agccattaaa aatttgatta atgctattca gcggctggat
     4381 gcgaataacg agaaagataa agtacttctg ctttctataa aaaatggtgt gcagacttat
     4441 caaaggcagg gtggtgatcc tgctggtcgt ttagatcctt tattaaaaga ggccagtaat
     4501 agtcttaaaa aagtttcacc atcagaaggt tcttctgaaa gtaaaaaata aatcacaaga
     4561 gctatgaaaa atatttatga tcaagaaaca ttggaagcat atttagtgga atttttaact
     4621 atgcctccta ttgtagtggc agagtttaat cgaccgagta attataaaat actaactgat
     4681 ttagctgcag aatacaaagt caatttagat atagtggttt tattggtagc tattaatcgg
     4741 gtatctatgg atgagttgga tcaatatttg gtagataatt tagatttaga ccgggccaag
     4801 gctagtgaat tggccactaa aatgagagat aattttttcc tgccaatcat aaacaaaata
     4861 gcttttttaa gctctcaacc atcacctgaa gactatgaaa ttattgaaaa tatttttgcc
     4921 aaacaactat taaatgaaat taatgctgac ccaattttaa ttaaagccct taatcaaaaa
     4981 atttttgctt ggttaagcga agatctagat ggcgctaaaa ggcggattga gaatgctatg
     5041 ctgaaaaatc aagaaattgt tactaaagaa aaaatcacaa tgaatgatga taaactacca
     5101 ggtactattg gtaattggtt acattgtttt acggccgaga aaggagctgc taattttgat
     5161 tcattaagta tttctgaatt tcttagttta tctcccaaca ctactcattt gacagttgaa
     5221 gagaaaaaaa aattaagtat tgtgctcaat acttatcgta atattaaatt ttttccagat
     5281 agcatgccta gtgatgatgg tagtggctgg gccattatac ctttaccggt tgtagaacca
     5341 tcatcgcaag ccagtaagcc caaggaggag ggatcaccag aggtatcttc atcaaagtca
     5401 gacatagaat cacctcaatt aagcagtgat tctgacagag aaaataaaat taatcaattg
     5461 caattattac ttaagcgtta tggtaccgac accatagaag gtcaggccat tatggaagaa
     5521 attgctaaat tgaaaggcag ttaaaattta tgtttgacta tctacaaaaa tatcaacaaa
     5581 ttccggccga aatcagagcc aaattagata atgcttcggt taaggccaat atccatcagc
     5641 ttgaacaaca atatcgagtt tctttattta atatagttat gaaagtaatg gtgcgagata
     5701 ttcctcttga tcgcctatct ttttatttag ctgaaaatga aggaatgaat aaagaacaag
     5761 ctcaaaaatt agtagctgac ttgcaagcca atgtctttag cttagtgcaa gattatttat
     5821 ctaagccgtt atatgaacca attaatattg accaattagt acaacataat cgtgatatct
     5881 cgccttcagc ttccactgag ccacataata taaatttttc ggaaactcta gaggtaaaat
     5941 taaatgaatt tgttagccat caaaaaatag ttaaaaaatc agctgagtta aatcgtttgc
     6001 gccaaatagc taaaacctac cttctggggg ttcgcaatca acaagctttt gttgatattg
     6061 ctactaagac ggttgccgaa ggggggttgg ggctatctgc tgataaagct ctttctttag
     6121 tggctgagct aaacaatatt aaacaaaatt tggagtttga agctagacag caagtaaata
     6181 ttcaaccgcc agctaaaccg atagataaac ttattaaaca gtccattgtt gaagattttg
     6241 agtcggaatt attagctaat ttgcgtcaaa tagacaaaca accacgttat gaccttaaga
     6301 ctgaatatga acttacggca ggaactaaac cagaaactaa acttttaacc tcaaccacgg
     6361 aggcagaaca ggagatagta aaacagccaa ctgttaaaga ggcggcagta aatcaaccag
     6421 cggcagtagt tgttcctcta gaccaaagag taactgatgc caagacgggt aaaattcgta
     6481 tggatgatgt taagtttatt cctaagaccc tgaccccaat tgatgaattg gccactatta
     6541 gtttgaaaaa atttcgttat ttaggtccaa cggccgcaga gagaactaaa aaaattaaag
     6601 agaaaattga ccttttaaca gagtatggtt atagtaagcg tttacagggt attaatgctt
     6661 ggcgtcatag cccacttaat ttactttatt taaaaattgg ccaactaagt attgaaaagg
     6721 gagtatcagc tgaagaaata ttaaccaaag gagctccaga gttaggagaa gatatcttaa
     6781 ctttagatga atttaaggct atactagagt taaataaaca gcttcgtttt tagaaatata
     6841 tgcagcaatt tacagtgcca caatttattg atgaggaaga taaaatttta ggtccggtta
     6901 ctactaggca atttattatt cttttggtcg ctgctggttt tatgtttgtg gaatataaaa
     6961 tttttgattt ttctttattc ttagtctccg ctttaataac ggccggagct tttatggccc
     7021 tggcttttat gaaaataaat ggccgacctt ttcatttttt tcttttaaac cttattcaaa
     7081 gtcttttttc ccctaaactt agagtatgga ataaaaattt tggcaaagtt gaaaatataa
     7141 ttgaatttga ttataaaaat attatcaaaa cacagccgcc agcagctaaa gttcgctttt
     7201 cttcttccag attggctgag ttgtcattaa ttgttgatac ttttggagct tataggggtg
     7261 aagctcagga gccagaggaa ttagctaata ataaaattga gttatgaata gtaagcgatc
     7321 taatacacta acatctactc agcaatattt aaaaatagct gatattaaac aggacacatt
     7381 agtcatgaaa gatggaactt tgcgggccgt attaatggtg tctagtgtta acttcgcgct
     7441 caaaagtgaa gaggagcagg aggctattat taatggttat atttcttttt tgaataatat
     7501 agaatttctt ttacagattg ttgttcaatc gcgcgaatta aatatcaatg gttatttaga
     7561 gatgatgcgt aaacgcgcta atgaacagac taatgaacta ttaaaaaatc aaactttgca
     7621 atatattgac tatgtcagtc agctagtgag cctaggcaag attatgagca aacattttta
     7681 tgtagttgtt ccctatagcc ctatatctga taaacgtaaa aatttttggc agtcattact
     7741 tgaagccttt agaccaattg acgtaattca tcttaaagaa gagcgattta tgttgctaaa
     7801 aaagcagctg gatagtcggg tagataatgt tgctagcggt ttgctttcta tgggattaaa
     7861 tgttgtgcgg ttagatacgc aggggctcat tgagttattt tataattcat ataatcaaaa
     7921 gacggcagct aatcagccat tagataatat agataaactt aatattaata atagttttta
     7981 gccatgttta atcttaattc caaaaaaaaa ttatcacctg atacccatga taggccatca
     8041 ctgggtaatc aaaatttgca gtttttatct gagcaggctg ctttagccga ggagttaatc
     8101 gctcaagaaa aggcttaccg ctatggcgta gtgaatatcc gtgatttaat tgctccagct
     8161 agtttggagg ttaagcctaa atatttatta ttaaatgggc aatatgtaag caccatgttt
     8221 gtggtaactt atcctcgtta cattaatgtg ggctggtttg ctcctatttt aaacttgaac
     8281 gaatcttttg atatcgccat gtttttctat cccattaagt catcaattgt tttgaaacaa
     8341 ttaaaaaata aggtcggtgt tttggaagct caaattagtg ctgacattga aaagggtaat
     8401 ccacgtgatc ccattagaga aacggctctg cgtgatgttg aggctttgcg tgatgattta
     8461 actcaaggta tagaaaaatt ttttcaattt gctctttatg ttacctttta tgctgaaact
     8521 gaagagaaat tagaacaatt ggctaatcaa ctagaagatt tatttggctc taggttagtt
     8581 tattctaagc gtgtttttta tcaagcagag caaggtttta attctacttt acctatgtgt
     8641 agtgatgagt tgaatattgc ctataacatg aattcttcac cggttgcttc ttctttccct
     8701 tttatttcta gcgaattaac aagcgataat ggcattttgt atggtattaa tcgacataat
     8761 aatagtttga ttttatttga tcgttttagt ttacctaatg ccaataatgt tgtttttgct
     8821 acttctggag ctggtaagag ttatgctata aagttagaag tccttagaac actgatgatg
     8881 ggtaccgacg tcattgttat tgatcccgaa tatgagtata aagctttaac tgaggcagtg
     8941 ggcggaactt atattaatat ttctttggct agcgaagcaa aaattaaccc cttcgatttg
     9001 cctaggccag ttggacaaga gactaaaact gaagatataa tccgaagtgc tgtcatcaca
     9061 gttaaagggc taataagaat tatgctgggt aatttaacga ctgaagaaga ttctattatt
     9121 gatatggctc tcttagagac ttatgccaaa aaggacatta ctccagacag tgatttaagc
     9181 actattgagc cgccgacaat gaaagacttt cagtatattc tagaaggaat ggagggtggt
     9241 gaaggtctag ctttaagact taaaaaatat accgagggca ctttttctag tttatttaac
     9301 agtccaacta atgtggagat gaataatcaa ttggtggctt tttcagttcg tgatttagag
     9361 gatgaattaa gaccactggc tatatatacg atcgttaatt atatttggaa tattgttcgt
     9421 agtgagctca aacgacgcat tttagttata gatgaagcgt ggtggttgat gcaatatgaa
     9481 gatagtgcta aatttattta tgctttagtt aagcgttgtc gtaaatatta tcttggagtt
     9541 acgactatta ctcaagatgt taatgacttt ttgttatcac cctatggcaa ggctatagta
     9601 actaattcag caatgcaaat tcttttaaaa caatcaccag cggctattga tttagtacaa
     9661 aaaactttta tgcttactga gggtgaaaaa tatctactat tagaaagcgg ggtgggggag
     9721 ggtatatttt ttgccggtaa tagacatgct gctattaaga tcgtggcttc ttatctggag
     9781 gatcaactca ttacctctaa tcctgaacaa ttattgaaga tggaaaaaga agctcagcag
     9841 aaccaagaaa aaataatgaa taaataattt accttttaat ttatgaaaat ttcttctaaa
     9901 atgcgcaatt ggttgctatt gggaatagta gtgattattt taataatttt gctttattta
     9961 ttattaagca aaaaattaga agtccaggca ccacaattaa acgaacaacc tattgtagag
    10021 gaatttagta ccccaactga aacaccttta gcaatattag aaacagataa agtggcaagt
    10081 aataatcagg taccattaga tgatcgggct cgaactgaag tgcaattaga aaaagaagca
    10141 tcagcctttg ttgaacgttg ggttagttta tctaatcaag ataattttag caatttggcc
    10201 attttaaaaa atcagatgac tgacagaatg attaactgga ccaataagta tattcaagag
    10261 aatatgccct catcgtctaa caataaacta tatatgggcg ttgaggggca agccatagta
    10321 gctaatggta atttaaagag tgagacggct acctctctta cagtggaagt aaagattaga
    10381 ggcaaagaga ctatagcttc aaccgtcaat caacctaaaa tattttatca agattatcaa
    10441 gttgatatgg ttaaatctgg cggggtatgg ttagtagata agcttacgtg gggggaacgg
    10501 caatatcagg aataatttta tcattattaa ggagggggga ataaaataac ccttataggt
    10561 tattttattg ttataatata tgttaaaacc taagtctaaa ttaattttaa gcttaacttt
    10621 attatgtttt attctgttag gttgttttac tgtcagatat tattataggg acaatcatta
    10681 tctaaaaata gtttttttaa atataggaca aggggatgct actctaatta ctactgctac
    10741 taaaaagcat attttaatag actgtgggcc agaccaagct attttaaacg cactggacca
    10801 agaattatcc tggtgggaca ggacaattga tattgttatt attagtcatg accatgctga
    10861 tcactggggt ggactaagtg acttggttaa gaaatataag attgaacaag taattctagc
    10921 taaaccatct cagatttcat cagagtttgc tagtgtttta aataatatta gtactcaaaa
    10981 cattaagata accaatttat atgcaccagc tattgtgaat ttagatgatg gtagtaaact
    11041 cagtattatt tggccaacgg aagctaatat tgctgaacag gataatatta ataatcagtc
    11101 attagtgatt aaatggcaat atggtcagaa atttttttta tggaccggag atctggaaag
    11161 tgacgctgaa gacgaattaa tggctaatta tacagtaaat gagcctatta atattttaaa
    11221 aatagctcat catggttcat ggactgcaac ttcacctagg tggcttaatt attggcagcc
    11281 tgatttagct gttattagtg ttggtgctaa taacagcctc ggccttccta atgaaattat
    11341 cctggatcgt cttaaacgac agggcgttca aatttggcgt actgatataa atggtaaatt
    11401 agttatctat actgatggac agacattatg gcgagctaat aaataagctt gcaatttttt
    11461 atacctggtt tataataaaa tatatcaata ttagctacaa caatatggcc aaaaaaagac
    11521 aagaacggac gttagttatt attaaaccag atgcccttaa tcgcagtcta gctggtgaaa
    11581 ttattcatcg ttttgaaaga aagggcttaa aaatagtcgg tcttaagatg attcagctag
    11641 atgatattat tttagctgaa cattacgctc atttagctga ccagccgttt tttccgcgta
    11701 ttgctaaatt tatgtcaaac tggcccgtta ttgttatggc cctagaagga ttagaagctg
    11761 ttaatagtgt acgtctactt accggtgaaa ccttgggtcg tcatgctgat gctggaacta
    11821 ttcgtggtga ttattctatg agtgtccagg ccaatattgt tcatgcctca gatagtgtag
    11881 agaatgctag aaaagaatta gcacgttttt ttaggccaga tgaacttttt gattgggatc
    11941 gcctggatag ccaagctatc tatgctgaag gggaaggaga agaagacgat gatgaataat
    12001 aagaattttt cgggctatag tataatggta gtacgcgtgc atggggtgca tgtagctccg
    12061 gttcaattcc ggatagcccg accaacaaag tttcaattaa cttaccaata tgaaagaaaa
    12121 attcactgaa gcaaatagat ttttagcaga aaaagaagca gaagtaatag aaaatattga
    12181 gtgccctgaa gataaggaag attttgaagg ccaaaaagtt ttaataattg gcggtcctga
    12241 tgctcatgga gttagcatgg cagttagttc agccctatat ttagaaaagc ggggagcaga
    12301 acctactatt tatgttggct caccggttat tcgcgaagga aggggagcta cacatccagg
    12361 ggctttttat tgtaagacta tccccaactt aaatgttgct aactatgatc gattaattat
    12421 tgttgacatt cctctagatt ttagggattt atctggctca gaaaaagcga ttattgagtt
    12481 agatgaacga cttcgtcaag ataggcagca aagagggcaa tcacctttaa aacattctac
    12541 tttttacctc gatcatcata gtaccacaca atttacagcg cctcatgact ccattttaat
    12601 taaaaatgta cccgtagctg aagcatgtaa gttaggaaat gacaaaactc ttattggtcg
    12661 aataggagct atttgtgacc gcgatccggc cactttaccc attacttcag aagaaatgat
    12721 gttagctaga ggacttgacg ctgccgtacg tcctgaccct gacgatgaac gtccttcatt
    12781 gccaaaaaat gctagcgaag aagaaaaaca aaattatcaa aaacaattac gcgaatggga
    12841 agatagagcc cagcaacgat tagaagaggc cgttaaacgg cttaaagctg aagattggag
    12901 ctattttaaa aaagaagcag aacgaattaa tcaaatagaa atacctactt ccagtggttt
    12961 tggacaaatt gctatcgtcg atactcaaaa tttgactagt aaacttggtg ttttaaagtt
    13021 gatggaaatg gctattgaaa atatggggat tgatgaaacg ccatacgcta ttggtgtctt
    13081 acgagaatta ggtgatataa aaatgggacg tgaaccggct gacgtaataa ctgtgattcg
    13141 tcactggacg cgtgaagatt taccctcagt agaagaggtc attactcatt attttggtga
    13201 agaattcatt gaaaagtata aaatatacgg agcaaaaaat gccaaaacct tacgattgcc
    13261 agtaaatgat caagagacgg ccaagctaac agcacagttg gttagggctt ttgcaggacg
    13321 tgaaatgcca gatttttctc aggtacgttc agtagtaatg tgtggtgatc ctaattcggg
    13381 taaatctgtt tattcaacta tttttcgtga agctttaaaa aatttaggta tacgggtagc
    13441 tcatttagat ttggacaaag ctgctcccac cccacaatgg tatttagagg cagaaattgc
    13501 cagcaaggaa gcagaattgc ttttctctca gggcaaatta tctccagaaa aattagctga
    13561 agctagaaaa aagttggacg aagcagctga aaagcgacgg gccatgaagc gaccttggtc
    13621 tcttgagttg gccgaagaag ccaagcaaga actattagaa gcagcacgaa acgaaaacaa
    13681 cgattttgtt attggtgata tcggtggggg aaaaattaag aaagatannn nnnnnnnnnn
    13741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnngaa aacaacgatt ttgttattgg tgatatcggt
    13861 gggggaaaaa ttaagaaaga tagtgaaggc aatattgtta aaattactcg tcttacagct
    13921 gaaaatgcta aaattttaga aggtgctgat gcggttatta ttgtttccaa taatcctcaa
    13981 ggcgcagcag aatggaaaaa attaattgaa acagggattg atcctgaaac tggtgtagaa
    14041 attaaacgag ataaaccgat tcaaattttg ggaatttatc aaagtacgtt agaaggtaat
    14101 gtccagaaaa tagctggtga taaagaaatt ggcattatta ctaaccttga tcgcagtaag
    14161 gctgagaaaa aatataatcc ggctattttt actaccgcta tttttgttag tgaggcagtg
    14221 caaaaacgaa gacaaaatat gaatgattag ttttgataaa aattattctt atgttattag
    14281 aaaaaatcga tgcattgtca gttgataaac aaccgaaatc agaaaaaata gaagataaga
    14341 ttggtaataa ggaattacca atttgggata aaatgccaga aattgtggag accttaaaaa
    14401 ctaataatcg gttaatttta gttgccgaaa ctggcagtgg taagactacc caagtacccc
    14461 aagccttatg ggctgagggc tttgctgagc aaggaacaat ttttgtagta gaaaatcgtg
    14521 tggctgtggc tgtagaaatt gctaaacggg ttgcggaaga gatgaaagta aaaattggtc
    14581 aagaggttgg ctatcttacc gggccagaaa aaaagagtag ccgtaaggct caaattatat
    14641 ttttaactag cggagttttt aaaaatatca ttcgtgaaga tcctaaaatg gagcaagcat
    14701 cggtagtact ttttgatgaa tttgatgaac gttatctact ttccgatatt ggtttggcat
    14761 tagtggagaa agctcaagat caagggagca tggttaaatt tggtcttatg tctgctaccc
    14821 taaatgctca aaaattttct gaacattttg gtggggctcc tatagtagaa gctaagggca
    14881 gaccatatcc ggtggaatct cattatgctg cagattctat atctttatat cgaatgcctg
    14941 aaaaaagtgc agaattagca ttatctattc atcaatctag ctctcatggg gatattctaa
    15001 tttttatgcc tggtaaacaa gaaattaaag ctacaattga tgaaatgctc agccgcaata
    15061 ttactggagc tactatttta cccttatata gtgagttatc acctcaagaa cgtcataggg
    15121 tttttgaaaa acaatcagga agaaaaataa tagtggcgac taatattgct gaacgcggtt
    15181 tgactataga tggaatacga tatgttattg attctggttt ggtacggatg aatgaatatg
    15241 acccagcagc tgataccact aaattaatga ctgtcccaac tgctcaagat tctctaagac
    15301 aacgtcaagg acgagctggt cgtactcagc cgggtgaatg ttatttttta attactgaag
    15361 atgaatttaa tcatcgtcct accactacta agccagaaat tatgagaact tctttaagag
    15421 aagtggtatt acaaattaag gctatgggct attcacgtga agaggactca attcgtttaa
    15481 ttgattcacc agataaaaag aattggcaga ttgctaaaaa tcaacttcgt ttattgggag
    15541 cccttgatcc acagaatgag actaaactat ccgaatttgg ttataaatta tccgagctat
    15601 cttgcgaccc acgagagggg acaatgctct tgcgaggttg tgaaatgggc tgtgggcaag
    15661 aaatggccct tattgcggct attcgtacca gtaaacggtt actttatcga cctcaagacg
    15721 agattgaaca agctgatgca gctcacaaac aatttgagat ttcaaattta tctgaccttt
    15781 taaatctagc caatgtatat gaacaggcaa aacgtatgaa ttttgattcc cgttggtgtc
    15841 gcaaaaatta tgtttcttgg acagcattac gtgaagtaca acagaattat ttacagcttc
    15901 taagccaaat taagaaatta ggttaccaaa taaatcaaga caaagctaag ccagagatta
    15961 tttgtcgagc tatggccact ggttttccgg ataaaatttt tgagaatatc ggccgtggct
    16021 ggtacagaaa tactacaaca ggcgaggagg cattgttagg gcgagagtcg tgtgttagcg
    16081 gtaatttgat tattgctaat gaattgatta caatacaaac tagaaggggc ggtgaactac
    16141 ccttaattac tttagctact aaattagaac caacttggct taataattag gagggatata
    16201 ataataaaaa tatgaatgaa gcaaaattta cccccgaggc ttatacctct caagaaacta
    16261 aggagcatga gcctaaaatt gagagattat caattaatga ttttgcccgt gaaattgggc
    16321 tagagcccga cgcccagggt cgcattactt ttgggccaga gcatgttcga ttagcctatg
    16381 aatatgctga aaaattagct cgcgacaaac atgctgaagc tattgtcatt gatggagtag
    16441 cttcaccggc agttattgcc agccttttac atggtgctca ccctgctgag ggttatttga
    16501 cttacatgca aaaagatcca gaaggaacaa tggttaaaaa tgaaataaaa gttttagaac
    16561 cgttaccgca aggagatggc agtggtcctg agggatttac ttggtttaag aaagaagccc
    16621 ctgattatac cttagttgaa tttgctctca gcggagattt taaggcggat gatttagcca
    16681 aggttattcc tcctgaggtt gacccaacaa aaccagtatt aatttccggt agaggtcctc
    16741 tttatttaac tcagacaatt gctgctgctt atcgacatta taaaggcgtt cccggcgtgg
    16801 gtttttatca gccagccagc aaatttggcc cagctaaaac tgaaatcggc atttctcata
    16861 acgaagatct accattaggc ttaaattttg gcgaaccatc agaaattggt tccgctaaag
    16921 aacaacttga gaaaggcatc aacgaaaact tggctcaaat taaagaaggt attgaaaaag
    16981 gtcaaatatc ttccgtcgag gtgcagggta aaacaataaa aattactttg gagaatggaa
    17041 caacatataa tatctcagtg cgagaagtaa taaagaatga atagctaaaa gattagctag
    17101 acaattttta taattacaac agcattaaaa ctatgcc
//
LOCUS       DAOOIS010000010        16481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_3388_length_16481_cov_35.730975, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000010 DAOOIS010000000
VERSION     DAOOIS010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16481
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3388_length_16481_cov_35.730975"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PK960_01750"
     CDS             complement(<1..643)
                     /locus_tag="PK960_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HPD07929.1"
                     /translation="MSAKKPVSTTSSNLKDIIKNQTADFTPSSLKVSAKAKSKSPVSK
                     RADQHFKKDNPALPPTEKEIRALINRVKPRGFVTETELLYLFPKLEDYVYDYELLLEE
                     LQKHSIKVIETGEGYLEIGQAKAKQEEKTRSILGDIKDIKKFDISELSADSVQMYLKE
                     IGRVPLLTTEEEIELAKRKDKGDREAERRLIEANLRLVVSIAKKFSGTRGLNLL"
     gene            complement(645..2474)
                     /gene="dnaG"
                     /locus_tag="PK960_01755"
     CDS             complement(645..2474)
                     /gene="dnaG"
                     /locus_tag="PK960_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPD07930.1"
                     /translation="MTNPSDDIKARLNIVEVIGEYIRLQPAGSNFKAKCPFHQEKTPS
                     LMVSPDKQIWHCFGCGRGGDIFAFVMEMEGLSFPEALRLLAPKAGIELKNYQGQDNSR
                     RNRLLDLLGAATTFYHKQLLANNPLARSAYHYLTVERGLSPDIINEWQLGWAPNDWQT
                     LSEAMKRLGYTDNEMLLAGLINQAPEGQRFYDRFRGRLMFPWHDVSGNIIGFSARLLP
                     QLDDGQTGKYINSPQTMLYDKSRHLFGLWFAKGAIKEAQEVVIVEGQMDCLTAHQYNF
                     KNVVATSGTALTEEHLKLLKRYTSNIVLAFDMDQAGLLAADRAIALGSQANLNIKVLH
                     LPDGKDPDEFIRRHTSAWAEVVKQARPIMDYYYELLQPQLKSDKVEERKMATIRFLKM
                     ISQLSTVVERDYWLKELGKTLNLEVPFLYEELAKLEKSQKVSANNVPLRPAPQTKPVD
                     LLLAWLIKFPNVLESLGEELEPEFLSDPRANQFYKNLIVYYNKNRQIDGQTVDYHEFM
                     DWLKANNGAITKEITSYIDGLFLLVDKDLSEATLEESLGEIARLTKVIKREFWHKQLA
                     AISRKIGDLEKDQGLSEQKKDLEINILLGEAEDLSQKLRAIDN"
     gene            complement(2467..2769)
                     /locus_tag="PK960_01760"
     CDS             complement(2467..2769)
                     /locus_tag="PK960_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07931.1"
                     /translation="MKIRLLTNNLPMTIPQLMRQAGYTYLPGRGSDDSYIRVLGRSPY
                     PRFHAYVQEENGQLIINLHLDQKRPGYGVSARHNGEYDGPVVEAEARRLSQFISHD"
     gene            complement(2773..2844)
                     /locus_tag="PK960_01765"
     tRNA            complement(2773..2844)
                     /locus_tag="PK960_01765"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2809..2811),aa:Arg,seq:ccg)
     gene            complement(2871..5951)
                     /locus_tag="PK960_01770"
     CDS             complement(2871..5951)
                     /locus_tag="PK960_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07932.1"
                     /translation="MKPKVIFMKPRPPRESEPNIADNCREDKDSPEVYGDQLLDESET
                     EGPGLLKEEVEESPDEASLEEVTEMIAKEIPEGPDKVTRAKIIGAAAYKTIASIVGVK
                     AITDWLGAGLGSLGLKIGQRTDVYKYFEQKKVTAQDRAKIDDVLEKLMEAAVVHYDFK
                     QVVYQDPKYVELVSEMDQLLKDYTQEEDAEAKEILAEKIKQKQAEKKAIAQQYEQLPN
                     QAENSAELKNLAMEYNKLIDNARSAAYYDLSADKRKAIDANWDNLSEAERDLYTLPNS
                     DKRTMKIRLGQILLGNLDANDNLKKRKAEDSQTILRDYVRGKVEATGLIKDGLNTAFT
                     LTGAGVFRGLAYATMAMVERGKSKNREFRKEYSWNQIGEKISADRRLFDLFISTMKET
                     YYGLSGRKFIQKYIIGPDGKLQRVSDRIKLRGLQATAIRAQSVGEIIRLFGIGSQTMA
                     AVITDGMSLERSLEIIADTFKGNVFQNMANNWYQNFRIDQRIAKSFKALQNQPSQGTS
                     KETEEVTTAVQAAQEKPASSDNDINLKKLEELDTNKISWLAAKGRPVKAGSSVSETLG
                     RSVSNQESMLLVSFNRQGEPIIYDGYDPNIIAPGAKVVDYGGRLVAIDTDATHLHFYD
                     QHYWNDFLPKPIEIKRGPIDDQHLLDALLSKQPQTTGTTPDSTAINNVVAGSDSLGKY
                     SDHPYFNLTTKGKIDYSDLVRGDNNVYHQFVTFNRPGGVFDNTTHSNQDLLSDIDRIY
                     NNQDLSVGDRIKILESIKQEITDPNLVNQVSDPGTRELLAGTYHRYADVIDHQIALLQ
                     HPDGQPVPQEWRDGIEGFTRWRDSNQSQGSQSEDLVNYNVANSVDVAVNAQTILTNPK
                     SSFTDIKTALLTILPEDHDKVKIGELNIVRHGDRFYINDHLLNKHSYHDLLFKPSELL
                     NKAVTSPKSVEELHTTLSAENAFSNLDASIKAGNDFLNPAIPFVDKINGLKAVIPENS
                     RMEFNDIIFLRANDQIYIKLTGDKVLPLSSPEVVERYLQDRKEALSQALNELEK"
     gene            complement(5957..6301)
                     /locus_tag="PK960_01775"
     CDS             complement(5957..6301)
                     /locus_tag="PK960_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07933.1"
                     /translation="MSRRKLSEEQIISGESQKLAFLVDSLNISQAAKDLLMEIISMME
                     PKDITQFAAVLEEKLASEQTAILEIELQNKLEALAIKYMAQEEAINHKSIVEIKKLIE
                     EQKLRNLRQTIK"
     gene            6438..6872
                     /locus_tag="PK960_01780"
     CDS             6438..6872
                     /locus_tag="PK960_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07934.1"
                     /translation="MKQADRKHILIISRHYPAYGQIKKIQQEYGEDCLITLDNQRFEG
                     LEDVLGRYYAGSYDNLAIVAPKEIIAGLYLLGVCPLVLSTIVVPEGGEKKAGDFQKND
                     GPLLRPIVQKIININYDINYQTIEDIETGVEEDEPIEEKNED"
     gene            complement(7021..7110)
                     /locus_tag="PK960_01785"
     tRNA            complement(7021..7110)
                     /locus_tag="PK960_01785"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7074..7076),aa:Ser,seq:gga)
     gene            complement(7131..7916)
                     /locus_tag="PK960_01790"
     CDS             complement(7131..7916)
                     /locus_tag="PK960_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPD07935.1"
                     /translation="MTKKVTRRHFTDQFLWTVIVVVLLAFIALWIYQKTMKPGTSKPT
                     STENQAMVADNLKTANQEALENIDLTLSEVPPLTSTDHLLGPIEAPAQLIIYTDFSDP
                     LAAQFVPIIKELKSKFNDDLVVAWRQHPLTNNPLANEAAVAIECAAKQGKFFDYAERI
                     ATISAQEQILPEWSALASELNLKVDDFNNCLTKPEVQATIDHWSAEAEALGAIGVPTS
                     FLNKKLLTGVYPLEDFTASDGQVKSGLVALVQEAIKANQGVVQ"
     gene            complement(7942..9405)
                     /gene="gatA"
                     /locus_tag="PK960_01795"
     CDS             complement(7942..9405)
                     /gene="gatA"
                     /locus_tag="PK960_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPD07936.1"
                     /translation="MDLHNLTLVQVRGLLDNQQITSRQLTDYYLKRLEKYDSYIHSCL
                     FIDYAEARRVADLADERLARGERGGVLGIPYIVKDNILTKGLKTTAGSKMLADYVAPY
                     DATVISRLKQAGAVLVAKANLDEFALGSSTENSAFGPTHNPYNLSMVPGGSSGGSAAA
                     LAADFCTFALGSDTGGSVRHPASLCGVVGYRPSYGAISRHGLVAMASSLDAIGILAHS
                     ATDVATIMGVIAGPDKYDATASLVLEAPTRVSQTINWTNIKIGWPAEYFGPGLDSEVA
                     DSLSQIKNLLLDKGATILPVNLPLTSYGVSAYTVINMAEVSSNLARFDGLRYGHQTGG
                     SNLLETYLNNRGNGFGAEAKRRIMLGTYFLSAGEGGSYYKKAQIVRAMISREFKEIFT
                     QVDYLFTPTTPTAAFPLGAKVNDPLSLYLEDSLLTPASLAHLPAVSIPIGWTSANLPI
                     GGQIIGKRRDDYKVLTLAQAIQDIIDLPSQSLTWETN"
     gene            complement(9412..9720)
                     /locus_tag="PK960_01800"
     CDS             complement(9412..9720)
                     /locus_tag="PK960_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/glutamyl-tRNA amidotransferase subunit
                     C"
                     /protein_id="HPD07937.1"
                     /translation="MDKDIIQKLAQLARLKVDNNEVKIYGRQLSAVLHHLNSLEQAMS
                     LISKYGGRDRVGKEHNDDINFSLADDEVKNWPKDEVAVSLKAASRNEDGFIIVPVIKS
                     "
     gene            complement(9724..11844)
                     /gene="ligA"
                     /locus_tag="PK960_01805"
     CDS             complement(9724..11844)
                     /gene="ligA"
                     /locus_tag="PK960_01805"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPD07938.1"
                     /translation="MNKDLSLIKQRIEKLRAEIEEARYAYHVLDKNLIDEGALDSLKN
                     ELFQLEQAYPQFITPDSPTQRVGGKALDKFAKVVHDYPMISLYDAFSEEDMLAWEERN
                     RAFFGGTLPDDYYVELKFDGLAISLRFESGLLVQGATRGDGQVGEDVTANIRTITSIP
                     LRLRRPSEEALSLIGFSLTDIKELYNFIDHKVVEIRGEAIITKKSLAEINRRYAQAGR
                     PLLANARNAVAGSIRQLDPKVTAERKLDFYTYDIWLPGLKRGELVRWRHQADQLVRLL
                     GFKTAQHNKLCHGLSEVFAYYRQIEKIRESLPFEVDGIVVKYDDLKLWDVLGVVGKAP
                     RYMMAYKFSAKQATTRLLDVRWQVGRTGVLTPTAILKPVKVGGVTISRATLHNFDEIN
                     RLSLKIGDTIIVERAGDVIPKIVGVLPKLRTGDERSIEPPKYCPFCGSVVQRVAGEVA
                     YRCPNSDCYAVTLRRLRHWASQEAAGWDGFGPKIIEQLLDNGLIKDIVDFYDLTVDQL
                     KILPGLGDKSANNLISIINRKKILPLNRLIYGLGIRQVGQETATTIANFLSTRLPNLS
                     HNVSPEEIFTVGRAVTLEEWQSLPDVGPKVAEALFSWFHDDHNYDLMHRLSMRGVAAN
                     WENKVGVQSLSGKTFVLTGRLTSLTRAQAEARIKQAGGRVSSQVSRQIDYVVAGEDPG
                     SKLTKAQNLGVKIINEAEFLALLA"
     gene            complement(11837..12505)
                     /locus_tag="PK960_01810"
     CDS             complement(11837..12505)
                     /locus_tag="PK960_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HPD07939.1"
                     /translation="MKIMNSVDRQFLFHKFLSRHSGQIMIVTFLLITGAFIVALSVAT
                     LVMMAINISRTQSDSVRAYFAADSGIERALAFDKLGGGIDRQNCGPGGKHYLCCDEPG
                     ECLFFDQLVKGHEEDGKVSGCFDCDFVEPEELISGGGQSYEIIYQGTNLDSSQLNLES
                     IGTSNGVRRAIELTIYRTIGCRPQCQDEETGEMYGCGYNGCNGFCGYCEEGQVCQNHF
                     CVYE"
     gene            complement(12480..13061)
                     /locus_tag="PK960_01815"
     CDS             complement(12480..13061)
                     /locus_tag="PK960_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPD07940.1"
                     /translation="MPGSKYKKLGFTLIEAMVSVAIFAILMTSAIDIFLSIIKSQRNT
                     LSSKNAQEAVNYALEIMSKELRMAKVDDGYCGAPQEVYAIFDENGQPATPSGPTIKFR
                     NYEGECVVYRLDNSAGRLVIIRNGRQAYMTPADITISDLSFYLQHHDITIGQPQVTVR
                     FIVSYFNTEAGQQIMQVQTSISSRSYENYEFGG"
     gene            complement(13048..13554)
                     /locus_tag="PK960_01820"
     CDS             complement(13048..13554)
                     /locus_tag="PK960_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPD07941.1"
                     /translation="MRKQQHPTIPAFSLVELLVAIAIIGIGLLGIASLATQNIRVQYF
                     NRNSLISAQLAQEGLELARNLRDNDWKSSSPITSSSSLAGLYGDLFTIDYLGNITEYS
                     QPEDVPFLSIDSNGFYSYAPGALTHFQRYLTTECQDNYCLLTCIVTWQAQGQSVNYEV
                     SDYLYAWQ"
     gene            complement(13561..14133)
                     /locus_tag="PK960_01825"
     CDS             complement(13561..14133)
                     /locus_tag="PK960_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPD07942.1"
                     /translation="MQVMRKKYQSASAFTLLELLVVLSIFVIIASLSIANYRGSNRES
                     QLKMATQELVANIKLAQSYALGSKEFVSRTDPRSGQVPSGGWGVHFSVYNPGEYEIVA
                     DFDGNHVWDSQRDGLFKEVELPEKISITKLIYRSSTVSNLDIFFEPPDPQTFLNQDKE
                     GNATIVLYDDLLATTSEVTVNFFGLVEITK"
     gene            complement(14135..15454)
                     /locus_tag="PK960_01830"
     CDS             complement(14135..15454)
                     /locus_tag="PK960_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07943.1"
                     /translation="MKIFKCIKIIGLIIWGANLLLGSTLIASAGEGDNLFGWAWNSEA
                     GWISFNNCVYGGGCSGYSYGVNLNEGSYALSGYAWSSNVGWISFNDSGIPGYSYPEGG
                     NPEHGAQLDPVTRRFSGWAQILSLGSDGWVKLAKDSHDSGPDYYVELLPDGKLAGWAW
                     NNMIGWISFNSADWSGNTVTYYVTGQAPFVPTAVTVSPDALSGCNTLNINWQSSLYAE
                     SYGVYRSGIASFTPTTSNLIGTTTNTNFINTDLALNTTYYYRIGASNFFGTVYTSTST
                     PGHTRAICGVQTISGEGDCLSGNPSIIELRWREPIIASGTTIVYYTVERCHVTETEDC
                     LRVLDSPFTDVATSSDCYHVVPTSFDNPPVHGVSSTPHCVGNNCHCWETFIGEDVAKQ
                     YVYKITAIDNYSQPGDETNPTALIQIFPCSREKSWQWEEHNPATSSK"
     gene            complement(15464..16276)
                     /locus_tag="PK960_01835"
     CDS             complement(15464..16276)
                     /locus_tag="PK960_01835"
                     /inference="COORDINATES: protein motif:HMM:NF018460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPD07944.1"
                     /translation="MGSLILGIIFSFLLGSALGSFVHCWAHRLYSGEGLRRPSYCPQC
                     GKRLAWYDNIPIFSYLRLKGRCSSCHKKIPRDYFWSEFLGGSLLAIAFLAAWQKVGGE
                     EGIWIAMHTVQGWLVVLQAIIGLVLLMLVFWTDYWWLSIFTRPLWIGGLVMIFVKWLA
                     GGSLVNMVVGGLFGSLFFALQFWASKGQWIGEGDIYLGLFLGIWLGWPLIVPAIFGAY
                     ILGAIVGLSLIIIGQKKWTSKLPLGVFLVPSSWVFFLWGQQWWQSYLHLLGW"
BASE COUNT         5201 a         3796 c         2708 g         4776 t
ORIGIN      
        1 ccagtaaatt aagacctcgc gtgccgctga actttttagc aatagagact accaatctaa
       61 gattagcctc aataagacgg cgctcagctt ctctatctcc tttatctttg cgtttagcta
      121 attcaatctc ctcttccgtt gttaaaagag gcacccggcc aatttctttt aaatacatct
      181 gcaccgaatc ggctgaaagc tcagagatat caaacttttt tatatcttta atatcaccta
      241 aaatagacct ggtcttttct tcctgcttag cctttgcctg tccaatttct aaatacccct
      301 cccccgtctc tataacttta atgctatgct tctgcaattc ttctaaaagt agttcgtagt
      361 catagacata atcttccagc ttgggaaaaa gatatagcaa ttcagtttct gtgacaaaac
      421 cacgcggctt tacccggtta attaaagccc gaatctcctt ctcggtaggc ggcaaagctg
      481 ggttgtcttt tttaaaatgc tgatctgcgc gtttagaaac aggactcttg cttttagcct
      541 tagcagacac ctttaaacta gacggcgtaa aatcagctgt ctgattttta ataatgtctt
      601 tcaaattaga agaagttgtt gatactggtt tcttagcgct catgttaatt gtctatagct
      661 cttaattttt gacttaaatc ttcggcctcg cctaagagaa tattaatctc taaatctttc
      721 ttctgttcac ttaatccctg gtctttttct aaatcgccaa tcttacggct gatagcggcc
      781 aactgcttgt gccaaaattc ccgtttaata actttagtta gcctggctat ctcccccaat
      841 gattcttcta aggtggcttc agataaatct ttatctacta aaagaaaaag accatcaata
      901 taactggtaa tttctttagt tatggctcca ttattggcct taagccaatc cataaattca
      961 tgatagtcta ctgtctggcc gtcaatttgt cggtttttat tataataaac aattagattt
     1021 ttgtaaaact gattcgcccg cgggtcactc aaaaattcag gctccagctc ttcacctaag
     1081 ctctcaagca cattgggaaa cttaatgagc caagctagaa gtaaatcaac cggcttggtc
     1141 tgcggtgctg gcctcagtgg aacattatta gctgaaacct tctgagactt ctctaactta
     1201 gctagctctt catagagaaa tggcacttct aaatttaaag ttttacccag ttcttttaac
     1261 caataatcac gctccaccac cgtagataac tgactaatca tctttaaaaa acggatagtc
     1321 gccattttac gctcttcaac cttatcactt ttaagctgag gctgtaaaag ttcataataa
     1381 taatccataa tgggtcgagc ttgtttaaca acctctgccc aggcactagt atggcgacgg
     1441 ataaattcat ccgggtcttt gccgtctggt aaatgaagca ctttaatatt gagattagcc
     1501 tggctgccta aagcaatagc cctgtccgcc gccagcaaac cggcttgatc catatcaaaa
     1561 gccaatacta tattactggt ataacgtttt aataatttta aatgctcttc agttaacgct
     1621 gtgcccgaag tggccactac atttttaaaa ttatattgat gagctgttag acaatccatt
     1681 tggccctcca caataactac ctcctgcgct tctttaatcg ctcccttggc aaaccataaa
     1741 ccaaaaagat gacggctttt gtcataaagc atggtctgcg gactattaat atatttacca
     1801 gtttgaccat catctaactg aggcaaaaga cgagcagaaa acccaataat attgccactg
     1861 acatcatgcc aaggaaacat aagacgcccc cgaaaacggt cataaaaacg ctgaccttca
     1921 ggcgcctgat taatcagacc agccaatagc atttcgttat ctgtataacc taaacgtttc
     1981 atggcctcgc taagagtctg ccagtcatta ggagcccaac ccaactgcca ttcattaata
     2041 atgtccggcg atagaccccg ctccaccgtt aaatagtggt aagctgatcg agctaaagga
     2101 ttattggcta ataactgctt atgataaaaa gtcgtggctg cccctaaaag atccaacagc
     2161 cgattacgcc gtgaattatc ttgcccttga taattcttta attctatccc cgccttagga
     2221 gctagtaacc ttagagcttc gggaaaactt agaccctcca tttccataac aaaagcaaaa
     2281 atatctccgc ctcggccaca gccaaaacaa tgccaaattt gcttatccgg cgagaccatc
     2341 aaagaaggag tcttttcctg atgaaatgga catttggcct taaaattgct ccccgctggc
     2401 tggagcctaa tatattcacc aattacctcc acaatattta accgggcttt aatgtcatca
     2461 ctgggattag tcatgggaaa taaattgact taaacgccgc gcttcggctt ccactaccgg
     2521 tccatcatat tccccgttat gacgagccga cacgccgtag cccggccgtt tttgatctaa
     2581 atgtaaatta attatcagct ggccattttc ttcttgaaca taggcatgaa atctaggata
     2641 aggcgaacgg ccaagaactc taatataact atcgtcactg ccacgaccag gcagataagt
     2701 ataaccagcc tgccgcatta attgcggaat agtcatcggt aaattattag tcaggagtct
     2761 aattttcata agcgccccta gagggaatcg aacccccatc gcaagctccg gaagcttatg
     2821 ttttatccat taaactatag gggcgtgtaa gcaatataca acaacttaac ctatttttca
     2881 agctcattaa gagcctggga taaagcttct ttccgatcct gaagatagcg ctccacaacc
     2941 tcaggagacg ataagggcag tactttatcg ccagttaatt taatatagat ctgatcatta
     3001 gcccgtagaa aaattatatc attaaattcc attctggaat tttcaggtat aaccgccttt
     3061 aaaccattaa tcttatcaac aaatggtatg gccggattca aaaaatcatt accagcttta
     3121 atagaagcat ctaaattgct aaaagcattt tcagcagata gagtagtatg aagttcttca
     3181 acagatttcg gactagttac cgccttattt aataactcac taggtttgaa gagaaggtca
     3241 tgataactat gcttatttaa taaatggtca ttaatataaa atcggtcgcc atgccgcact
     3301 atatttagtt caccaatctt aactttatca tggtcctccg gcaaaattgt tagaagagct
     3361 gtttttatat ccgtaaaact agactttgga ttagtaagaa ttgtctgagc attgactgca
     3421 acatcaacag aattcgcaac attataatta accaagtctt cagactgact accctgtgat
     3481 tggttagaat cacgccaccg cgtaaatcct tctataccat cccgccactc ctggggaacc
     3541 ggctgaccat ctggatgttg taataatgca atctgatgat caataacatc agcataacga
     3601 tgatatgtcc cagctaaaag ttctcgagtc ccagggtcgc taacctgatt tactaagtta
     3661 ggatcagtaa tttcctgctt aatggattct aagattttaa ttctatcacc aactgataaa
     3721 tcttgattat tatatatacg gtcaatatca ctaagtaagt cctgattaga atgagtagta
     3781 ttatcaaaaa caccacccgg acgattaaag gtaacaaact gatggtaaac attattatca
     3841 ccacgtacta aatcagaata atcaatttta cccttagtgg ttaggttaaa gtagggatga
     3901 tcagaatact taccaaggct atcactacca gctactacat tattgatggc cgtagaatct
     3961 ggagttgtcc cggtagtttg aggttgctta ctaagtaaag catctaataa atgctgatca
     4021 tcaataggtc ccctttttat ctcaattggt ttaggtaaaa aatcattcca ataatgttgg
     4081 tcataaaaat gaagatgagt agcatcggtg tcaatagcta ccaagcggcc accataatcc
     4141 actactttgg ctcccggagc aataatattg gggtcatagc catcataaat aatgggctca
     4201 ccttgtcggt taaaactaac aagcaacata ctctcctgat tactgactga ccgaccaaga
     4261 gtctcagata ctgagctacc tgctttaact ggacgaccct tagcagctaa ccagctaatt
     4321 ttattagtat ctaactcctc taactttttt aaattaatat cattatcaga agaagctggc
     4381 ttttcttgag ctgcctggac tgctgttgta acctcttctg tctctttact agtaccttga
     4441 ctgggctgat tctgtaaagc cttaaatgat ttagctatac gttggtcaat gcgaaaattt
     4501 tgataccaat tgttagccat attctgaaaa acattaccct taaaggtatc ggcaataatc
     4561 tctagcgacc tctctagact catcccgtca gtaataactg cggccatggt ctggctgcca
     4621 ataccaaaca aacggataat ctcacccaca gattgagccc taatagccgt agcttgcaaa
     4681 ccccgtaatt taatcctgtc agaaacgcgt tgtaatttac catctggacc aataatatat
     4741 ttttgaataa atttccggcc acttaaccca tagtaggttt ctttcatggt gctaataaat
     4801 aaatcgaaaa gacgcctatc tgctgaaatt ttttcaccga tctgattcca agaatactct
     4861 tttctaaatt cacgattttt agatttaccg cgttccacca tagccatagt tgcataagct
     4921 aaacctcgaa aaactccagc cccagtaagg gtaaaagctg tgtttaggcc atctttaatt
     4981 aaaccagtgg cctcaacttt accacggaca taatcgcgca aaatagtctg actatcttca
     5041 gccttacgtt tctttagatt gtcatttgca tctaaattac ccaatagaat ctgccccaac
     5101 cttattttca tcgtccgttt atcagaattg ggcaaagtat aaagatcgcg ttctgcttct
     5161 gaaaggttat cccaattggc atcaatagcc ttacgtttat cagccgataa atcataataa
     5221 gcagctgacc gagcattatc aatcaattta ttatattcca tggccaaatt cttaagctct
     5281 gcactattct cagcctgatt tggcagttgc tcatattgtt gagctatggc ctttttctca
     5341 gcctgcttct gtttaatctt ttccgctaaa atctctttgg cttcagcatc ttcttcttgg
     5401 gtataatctt ttaacaattg atccatttcg ctaactaatt caacgtattt cggatcttgg
     5461 tacactacct gcttaaaatc ataatgcacc acggccgcct ccattaattt ttccaagaca
     5521 tcatcaatct tagcgcggtc ttgagccgtt accttctttt gctcaaaata tttataaaca
     5581 tcagttcgct ggccaatctt caaacctaat gagcccaatc cagcccctaa ccaatcagta
     5641 atggccttaa cgccaacaat tgaagcaata gttttataag ccgcagcacc aattatcttg
     5701 gcccttgtta ccttatccgg tccttcggga atctctttag ctatcatctc agtaacttct
     5761 tctagagaag cctcatcggg cgactcttcc acctcttcct ttagcaaacc tggcccttct
     5821 gtctcacttt catctaataa ctggtcacca taaacttcag gtgaatcttt gtcttcgcgg
     5881 caattatcgg ctatattagg ttcggattcc ctagggggcc taggtttcat aaaaattact
     5941 ttaggtttca taaaaattac ttaatggttt gacgcaaatt gcgtagcttc tgttcctcaa
     6001 ttaatttctt aatttcaact atactcttat gattaatggc ctcctcttgg gccatatatt
     6061 taattgccaa agcctccaat ttattctgta gctctatttc caatatagct gtctgctctg
     6121 aagctaattt ttcttctaaa acggctgcaa attgagttat atctttcggt tccatcatag
     6181 aaataatttc cattagcaaa tccttagccg cttgtgaaat atttaaacta tccactaaaa
     6241 atgctaattt ttgactctca ccactaataa tttgctcttc ggataatttt cgtctagaca
     6301 taaaattatt attatttatt tatagattag cctaaatttt ttaaaattac aaggttattt
     6361 ccttccgcta aatattgaca tttttctcta atcacgataa aaaataataa agttctttac
     6421 aataaggagg aagaaaaatg aaacaagccg acagaaagca cattctaatt atttctaggc
     6481 attatcccgc ctatggacag attaaaaaaa ttcaacaaga atacggcgag gattgcctga
     6541 ttaccctgga taatcaacgt tttgagggtc tagaagatgt tttaggccgt tattatgccg
     6601 ggagttacga taacttagcc atagtagcgc caaaagaaat catcgctggc ctctacttgc
     6661 taggtgtctg ccctctcgtc ctttcaacca tcgttgtgcc cgaaggagga gagaaaaagg
     6721 caggagattt tcagaaaaat gacggtcctc tccttcgacc cattgtccaa aaaataataa
     6781 acatcaatta tgacatcaac taccaaacta ttgaggacat tgaaactggt gtagaagaag
     6841 atgaacccat agaggaaaaa aacgaagact aataaagaca ttgcaaccca cttaaattat
     6901 taagtgggtt tttaaataga tgaaacactt ttagagtacc ataggtactt agcaagataa
     6961 catacccacc ttatagatat agagaggaat gaatactttt aattagtact tagacaatgc
     7021 tggcggaagg acggggattc gaaccccgga ggcctgtgac agcctactcg ctttccaagc
     7081 gagcgcactc gaccactatg cgatccttcc ttctccctcc ttgtaacacc ctactgcacc
     7141 accccctgat tagccttgat ggcttcttgg actaaggcta ctaatccgct ttttacttga
     7201 ccatcactcg cggtgaaatc ctctagaggg taaactccgg ttaacaactt tttatttaag
     7261 aaactagttg gtactccaat agcacctagt gcctccgcct cagctgacca atgatcaatt
     7321 gtagcctgaa cctctggctt cgtcaaacaa ttattaaaat catcgacttt aaggttaagt
     7381 tcgctggcta atgctgacca ctctggtaaa atttgttctt gagccgaaat agtagcaatc
     7441 ctctctgcat agtcaaaaaa tttaccctgc ttcgctgcac attcaatagc taccgcggct
     7501 tcgttagcta aaggattatt agtcaatgga tgttgacgcc aggccaccac taaatcatca
     7561 ttaaatttac ttttgagctc tttaataatc ggcacaaact gagcagctag tggatcacta
     7621 aaatcagtat agataattaa ctgggccgga gcttctatcg gacccaataa atgatcagtg
     7681 gaagtaagag gcggaacttc agataaagtt aaatcaatat tctctagggc ttcttgatta
     7741 gctgtcttca agttgtcggc caccatagcc tgattctcgg ttgatgtagg tttagatgtg
     7801 cctggcttca tagttttttg gtaaatccaa agtgcaataa aagctaataa aactaccact
     7861 ataaccgtcc aaagaaattg gtcagtaaaa tgacgacgtg ttactttttt tgtcataaat
     7921 ataatttaga ttgttatcta attagtttgt ttcccatgtt aatgattgac tgggcaaatc
     7981 tattatatct tgaatggcct gagctaaagt caacacctta tagtcatctc gtctttttcc
     8041 aataatctgc ccaccgattg gtaaattggc tgaagtccac ccaatgggta tagaaactgc
     8101 tggcaagtga gctaaagagg caggagtaag gaggctatct tctaaataaa gacttaaggg
     8161 gtcatttact ttggcaccca gaggaaaagc agccgtaggt gtggtaggag taaaaagata
     8221 atccacttga gtaaagattt ccttgaattc tcgactaatc atcgctctga caatttgcgc
     8281 ctttttataa tacgaccccc cttctccagc acttaaaaaa taagtaccta acataatgcg
     8341 acgtttagct tctgctccaa atccattgcc tcgattattt aaataagttt ccaataaatt
     8401 actacctcct gtctggtggc cataccgtaa tccatcaaaa cgtgccaaat tggaacttac
     8461 ttcagccata ttaattaccg tataagccga cactccatat gaagtaaggg gtaaattaac
     8521 aggtaaaata gttgctcctt tatctaataa gagatttttt atctggctta aactatcggc
     8581 tacttcgcta tcaaggccag gaccaaaata ttcagccggc cagccaattt taatatttgt
     8641 ccaatttatt gtttggctaa ctctcgtagg tgcttctaaa actaatgaag cagtagcatc
     8701 atatttgtct ggaccagcaa ttactcccat aatagtagca acatcggtgg cggaatgagc
     8761 caaaatacca atagcatcaa gggaactagc catagcaact aaaccatggc gcgagatggc
     8821 accatagctt ggcctataac caactacgcc gcataaagaa gccggatgcc gcactgatcc
     8881 accagtatca ctacctaggg caaaagtaca aaaatcagca gccagagctg cagctgaacc
     8941 gccagaagag ccccccggca ccatagataa attatatggg ttatgggtag gtccaaaggc
     9001 actattttcc gtagaagacc ccagggcaaa ttcatctaaa ttggctttag ccactaaaac
     9061 tgccccggct tgctttaatc gactaatgac tgtagcatca taaggagcta cataatcggc
     9121 taacatttta gaaccagccg tggtttttaa acccttagtt aaaatattat ccttcactat
     9181 ataaggtata cccaataccc ctcccctctc tccccgtgct aaccgctcgt cagctaaatc
     9241 tgctactcgg cgagcttcag cataatctat aaaaagacag gagtgaatat atgaatcata
     9301 cttttctaaa cgctttaaat aatagtcagt aagttgccga ctggttattt gttgattatc
     9361 taataagccc ctaacctgaa ctaatgttaa attatgtaaa tccataacag cctatgattt
     9421 tattacggga acgataataa acccatcctc atttcgagat gctgccttga gacttacagc
     9481 tacctcgtcc ttgggccaat tttttacttc atcgtcagct aaagaaaaat taatatcatc
     9541 attatgttcc ttacctacgc gatctctacc gccatattta ctaattaagc tcatagcttg
     9601 ttctaaacta ttgaggtgat gcaaaactgc cgacaactga cggccataaa tttttacttc
     9661 attattatca acttttaatc tagccagttg ggctaacttt tgtataatgt ctttatccat
     9721 aaattaggct aataaagcca aaaattcagc ttcattaata attttgacac ccagattctg
     9781 ggccttggtt aatttactgc ccgggtcctc accagccact acataatcaa tttgacggct
     9841 aacctgactg cttacccgac caccagcctg ctttattcta gcttcggcct gagcccgtgt
     9901 caaactagta agccgaccag tcaaaacaaa agtcttacca ctaagagact gaacgcctac
     9961 tttattctcc caattagcag ctaccccgcg catactcagg cggtgcatta aatcataatt
    10021 atgatcatca tgaaaccaac taaataaagc ttcagccacc ttaggcccaa cgtctggtaa
    10081 actttgccac tcttcaaggg taactgcccg gccaacggta aatatttcct cgggcgatac
    10141 attatgagat aaattgggca gacgagtaga caaaaaatta gctatcgtcg tcgccgtctc
    10201 ttggccaacc tgccgaatac caagaccgta aataagtcta tttaaaggta aaatcttttt
    10261 tcggttaata attgatatca aattattagc tgatttatct cccaagcctg gtaaaatttt
    10321 tagctgatca actgttaagt cataaaaatc aacaatatct ttaattaggc cattatctaa
    10381 cagctgctca ataatctttg gtccaaaacc atcccaaccg gcagcttcct gactagccca
    10441 atgtcgtaag cgccgcagag taacagcata acaatcagaa ttaggacacc gataggctac
    10501 ctccccagct accctttgaa caacgctacc acaaaaggga caatatttag gaggttcaat
    10561 agaccgctca tcaccagtac gtagtttagg taaaactcca acaattttag gaataacatc
    10621 accggctcgc tcaacaataa tagtatcacc aatctttaga gataaacgat ttatttcgtc
    10681 aaaattatgt aaagtagcgc gagaaatagt aacaccgccg actttcactg gttttaagat
    10741 agccgtcggt gttaataccc cggtccgtcc cacctgccac cgcacatcta gtaacctagt
    10801 agtcgcctgc ttagcactaa atttataagc catcatatac cgtggtgcct taccaactac
    10861 ccccaaaacg tcccataatt ttaaatcatc atatttaacc acaataccat ctacctcaaa
    10921 gggcaaggat tctctaattt tttcgatctg acgatagtaa gcaaaaacct ctgaaaggcc
    10981 atggcataat ttattatgtt gagctgtttt aaatcccaac aacctaacta actgatcagc
    11041 ttgatgtcgc cacctaacta gctctccccg cttaaggccg ggcaaccata tatcataggt
    11101 ataaaaatct aattttcgct cagctgtaac tttagggtca agttgacgaa tggaaccagc
    11161 tacagcattg cgcgcattag ccaagagtgg acgtccagct tgcgcatacc gacgattaat
    11221 ctcggccaaa ctttttttcg taattattgc ctctcctcta atttcaacaa ctttatggtc
    11281 aataaaatta taaagttctt taatatcggt caagctaaaa ccaattaaag aaagtgcctc
    11341 ttcacttggc cggcgtaaac gtaaaggaat gctagtaatt gtcctaatat tagcagttac
    11401 atcttcaccg acttgcccat caccccgtgt agccccttgg actaaaagtc ctgattcaaa
    11461 cctcaaacta atagccaaac cgtcaaattt aagctccaca taataatcat ctgggagtgt
    11521 ccccccaaag aacgccctat tccgttcctc ccaggccaac atatcttctt cagaaaaagc
    11581 atcataaagg gaaatcatag gatagtcatg aaccacctta gcaaatttat ctagagcctt
    11641 accacccacc cgctgagttg gtgaatcggg agtaataaac tgtggataag cctgttctaa
    11701 ttggaataat tcatttttta gactatctaa tgcaccttca tcaattagat ttttatctaa
    11761 aacatgatag gcataacgtg cctcctcaat ctcggcacgt aatttttcaa ttctttgttt
    11821 aattaaagat aaatctttat tcatagacgc aaaaatgatt ttgacaaact tgtccttctt
    11881 cacagtaccc acaaaaacca ttacaaccat tatagccaca tccatacatc tcacccgtct
    11941 cttcgtcttg acattgtggt ctacagccaa tggtccgata aattgttaat tcaatagccc
    12001 gccttactcc attagaagtc cctatacttt ctaggtttaa ctgactagaa tctaaattag
    12061 ttccttgata aataatttcg taagattgcc caccaccaga aataagttcc tcaggctcta
    12121 caaagtcgca atcaaaacaa ccagatactt tgccatcttc ttcatgaccc ttaactagct
    12181 gatcaaaaaa taaacattca cccggctcat cacaacataa ataatgtttt ccacctggtc
    12241 cacagttttg acgatcaata ccacctccta atttatcaaa agccaaagct ctctcaattc
    12301 cgctatcagc agcaaaatag gcacgcactg aatcactttg agtacgacta atattaatag
    12361 ccatcattac caaagtagca actgacaagg caacaataaa agcaccagta ataagtaaaa
    12421 aagtaacaat cataatctgt ccagagtgcc tggataaaaa tttatgaaat aaaaattgtc
    12481 tatccaccga attcataatt ttcataagac cttgaagata tagaagtctg aacctgcata
    12541 atttgttgac ccgcctcagt attaaaataa cttacaataa aacggacagt aacctggggt
    12601 tgaccaatgg ttatatcatg atgttgtaaa tagaaactta agtcactgat agtaatatcg
    12661 gctggagtca tatatgcctg ccggccatta cgaatgataa ctaaccggcc agctgagtta
    12721 tctaatctat aaaccacaca ttcaccttca tagttacgaa atttaattgt aggtccgctt
    12781 ggtgtagctg gctgaccatt ttcatcaaat atagcataca cttcctgagg tgcgccacaa
    12841 taaccatcat caaccttagc catccgcagt tctttagaca taatttctag ggcataatta
    12901 acagcctctt gagcattttt agaagacaaa gtattgcgtt gtgacttaat aatggataaa
    12961 aaaatatcaa tagcgctggt cattaaaata gcaaaaatag ctactgagac catggcctct
    13021 attaaggtaa aacctaattt tttatattta ctgccaggca tataaataat cacttacctc
    13081 atagtttact gattgacctt gggcctgcca agtaactatg caagttaata aacaataatt
    13141 atcttgacat tcagtagtta aatatctttg aaagtgagtc aatgctcctg gtgcataact
    13201 ataaaaacca ttgctatcaa tacttaaaaa aggaacatct tcaggttgag agtattcagt
    13261 aatattaccc aaataatcaa tggtaaacaa atcaccgtaa aggccggcca aagaagagct
    13321 agaggtaata ggacttgatg acttccaatc attatcgcgc aaattacgag ctagttctag
    13381 gccctcctgg gccaattgtg ctgagattaa actattgcga ttaaaatatt gaacacgaat
    13441 attttgagta gctagagagg ctatgcccag caaaccaatg ccaataatag ctatagccac
    13501 taataactcc actaaagaaa aagctgggat tgtggggtgt tgctgcttcc tcatgtattt
    13561 ttatttagtt atctctacta aaccaaaaaa gttaaccgta acctcgctgg tggttgccaa
    13621 taagtcgtca taaagtacga tggttgcatt accctctttg tcttgattta gaaaagtctg
    13681 agggtcgggt ggttcaaaaa aaatatctaa attgcttact gtcgatgaac gataaataag
    13741 cttagtaata ctaatctttt ctggtaactc aacttcctta aacagcccat ctctctgact
    13801 gtcccaaaca tgattgccat caaaatcggc tacgatttca tattccccgg ggttataaac
    13861 tgaaaaatgc accccccaac caccactagg tacttgccca gacctaggat cggttctgct
    13921 aacaaactct ttagaaccta aagcataact ttgagccaac tttatattag caactagttc
    13981 ctgggtggcc atctttaatt gcgactctcg attacttccc cgataattag ctatgctaag
    14041 cgaagcaata atgacaaaaa tgctaagaac tactaataac tctaataaag taaaagctga
    14101 ggcactttgg tatttttttc tcattacttg catattattt actagaggta gccggattat
    14161 gctcctccca ctgccaactt ttttcccgtg aacaaggaaa aatctgaatc aaagcagtcg
    14221 gattagtctc atcgccgggt tgactataat tatcaatagc tgtaatttta taaacatact
    14281 gcttggctac atcttcccct ataaaagttt cccagcaatg acaattattg cccacacaat
    14341 ggggggtgga gctaacacca tgcactggtg ggttatcaaa actagtagga acaacgtgat
    14401 aacaatcact gctagtggcc acatcagtaa atggtgaatc aagaactctt aggcaatcct
    14461 cagtctcggt cacgtggcaa cgttcaactg tataatagac aatagtagtg cctgaagcta
    14521 taattggctc ccgccatcgt aattcaataa ttgaaggatt accgcttaga caatcacctt
    14581 ctccactaat agtctgaaca ccacaaatag ctctggtatg tccaggagtg ctagtggatg
    14641 tatagactgt gccaaaaaaa ttgctagctc caatacgata ataataggta gtatttaaag
    14701 ctagatcagt attaataaaa ttggtattcg tggtagtacc tattaaatta ctagtagttg
    14761 gagtaaatga ggcaataccg ctacgataaa ccccataact ctcagcatat aaactgcttt
    14821 gccaattaat gtttagggtg ttacaaccag ataaagcatc agggctgacc gtcactgccg
    14881 ttggcacaaa aggcgcttga ccggtaacat aataagtaac tgtgttgcca gaccaatcag
    14941 ctgaattaaa agatatccag ccaatcatat tattccaggc ccaaccagct aattttccat
    15001 ctggtaatag ctctacataa taatctggcc ccgaatcatg gctgtcttta gctaatttaa
    15061 cccagccatc agaccctaaa gataaaattt gcgcccagcc actaaaacgt cttgtgacag
    15121 gatcgagctg agctccatgc tcaggattac caccctccgg atagctatac ccgggtatac
    15181 ctgaatcatt aaagctaatc cagccaacat tagaactcca agcataacca gataaagcat
    15241 aactcccctc attgagatta actccatagc tataaccact acaaccacca ccataaacac
    15301 aattattaaa actaatccat cccgcttctg aattccaggc ccaaccaaat aaattgtctc
    15361 cctctccagc tgaagctatc aaagttgagc ccaataataa attggctccc caaataataa
    15421 gcccaataat tttaatacat ttaaaaattt tcatttattt ctcttaccaa cctaataaat
    15481 gaagatagga ctgccaccat tgttgacccc acagaaaaaa tacccaacta ctaggaacca
    15541 aaaatactcc caatggtaac ttagaagtcc attttttctg tcctattata attaatgata
    15601 aaccaactat cgcccctaaa atataggcgc caaaaatagc aggaacaatt aacggccaac
    15661 ccagccaaat gcccaaaaat aaaccgagat aaatgtctcc ctcaccaatc cattgcccct
    15721 tagaagccca aaattgaaga gcaaaaaata gactgccaaa aagtccacca actaccatat
    15781 ttaccagcga cccaccagct agccatttta caaaaatcat taccagccca ccaatccaca
    15841 agggccgagt aaatatagat agccaccaat agtcagtcca aaataccaac attaaaagaa
    15901 ctaaaccaat tatagcctga agaactacta accacccctg tactgtatgc atagctatcc
    15961 atatgccttc ttctccccct actttctgcc aggcagcaag aaaagcaata gccaataagc
    16021 taccacctag aaattcactc cagaaataat ctctaggtat tttcttatga cacgacgaac
    16081 atcgtccctt aagtcttaga taactaaaaa taggaatatt atcgtaccac gctaaacgct
    16141 taccacattg tggacaataa gaaggacgac gcaaaccttc cccactatat aaacgatggg
    16201 cccaacaatg gacaaaacta cccaaagctg aacccaataa aaaactaaaa attataccaa
    16261 gaattaatga tcccataagt gataactcct actattatag caataatcaa ttatgggggc
    16321 aactaatatt aataacaaaa atagaagcca ccaactggct tctattttaa accacacttt
    16381 ctcattatta tttttaatcc atgctagatt cagtagctgt cttagtagct gaactaccct
    16441 ctaaataata agctatccta taatgagtag catctacctt a
//
LOCUS       DAOOIS010000011        14059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_4126_length_14059_cov_43.990574, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000011 DAOOIS010000000
VERSION     DAOOIS010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14059
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4126_length_14059_cov_43.990574"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..473
                     /locus_tag="PK960_01840"
     CDS             51..473
                     /locus_tag="PK960_01840"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPD07945.1"
                     /translation="MPQHQKWWSKLQHPLFITIVSCIIVILLILGLLKNIRQLFNAHR
                     EINQIKTEIDNLDKKNTEISYLINYLQSKEFLEEQARLNFGLQKPNEQIIVVKQSATD
                     TTTNLVNSPVFNVPAPPQPKLAVSSNPKKWFDYFFAPQ"
     gene            complement(485..557)
                     /locus_tag="PK960_01845"
     tRNA            complement(485..557)
                     /locus_tag="PK960_01845"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(522..524),aa:Thr,seq:cgt)
     gene            complement(561..632)
                     /locus_tag="PK960_01850"
     tRNA            complement(561..632)
                     /locus_tag="PK960_01850"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(597..599),aa:Gly,seq:ccc)
     gene            712..2211
                     /locus_tag="PK960_01855"
     CDS             712..2211
                     /locus_tag="PK960_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPD07946.1"
                     /translation="MSSSHNIIFLLKHHFLFLSVIIISILPGVVVADSSVSSTSSVIG
                     RASWYNFRPGLFAASNIYSPGQKVRVYHDASGKYVDVVINDYGPDPTIHPDRVIDLQK
                     EAFAQLAPLGAGVITVRLEPLPQGENLAIISQPVSSLAVANASTSPKVVSNQTSRDLS
                     KEQAALTQFTKLYGRLPKDSVDWQIINDIAYNNLKRDLNKEKLALTQFTKLYGRLPKD
                     SVDWQIIKDIAYYQKLPKTNNNFKAVNISNKEAAAAIVLDGDSGQVLWEKNATQVLPI
                     ASLTKLVAVKTFLDLKPDLDKVVTYSQEDEKEIGKYCTPSEAAMVNLQDGDQVKIRDL
                     VYSALIGSANNAIESLVRLSGLTRDEFINKMNANAKKWGATSTKFVEPTGLSPDNVST
                     AKDYALILKNISQDATIKKISTTASYQFSTINTKQRHTIKNTNKLLREGNFSLETSKT
                     GYLTEAGHCLAMAAQQQNTKQHFIVVTLGSPTWSKSYEVASRLLTTALN"
     gene            2221..2901
                     /locus_tag="PK960_01860"
     CDS             2221..2901
                     /locus_tag="PK960_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD07947.1"
                     /translation="MIRFINASKFYPPQTKALDNLSLHIYPGEFVSIVGQSGSGKTTI
                     VKCLIGEEKLDEGRVIVGDWDITHISNREIPYLRRQIGVIFQDFKLLPKKTLAENVAF
                     ALQVCGKSPKKIKAIVPSVMKIVGLTEQMNRYPNEVSGGEQQRAAIARALVYQPKILV
                     ADEPTGNLDSINAEEIVNLLLKINDLGTTVILVTHNREIVNNLNRRVISISDGRLVND
                     QARGKYII"
     gene            2908..3819
                     /locus_tag="PK960_01865"
     CDS             2908..3819
                     /locus_tag="PK960_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPD07948.1"
                     /translation="MFLSLARIIKFGFQDVWRNFWLSLATIMILVLTLFSVDLLLALR
                     IIGQAAVDNLKNRVDVSIYLKQGVPEDKILNLKARLANLNNVQEVNYISQSAALESFQ
                     RRHQSDPEILQALQELDQNPLAPTLTVKPKDPDHYEELIEELDQIEDPIIESRNFQDN
                     KKLLDKINYITDKINDVAIIISSIFLLIMILVMFNSVRLAIYSHRQEIVIMKLVGASN
                     AFIKAPFLFSSLIYTLVGVVLTAVIFYAFLTVLQPYLDTFFFGYNIDLITYFNSHWLQ
                     IIGLQFAGAAMVNLLASYIAVGKYSKI"
     gene            3825..4517
                     /locus_tag="PK960_01870"
     CDS             3825..4517
                     /locus_tag="PK960_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPD07949.1"
                     /translation="MLSQKQEKLIKSLHTNKGRNETNLWLVEGAKCLELAGSAVQFKF
                     GPKDSHHFAQLTTTDTPPTIAGLAIPPKFQLDDITARRTILVLDHLQDPGNVGTIIRL
                     AGAFDASLILCECVDPGNPKVIRSSAGLVFATPWIPLPPRIALKWLSEVNYPIYRLEN
                     KAGAKELNIRTHKFLSADKIIIIVGSEGQGIKLKIPGQRVAIKHNPTVDSLNVAAAVA
                     IALFLRYQKNNK"
     gene            4519..5568
                     /locus_tag="PK960_01875"
     CDS             4519..5568
                     /locus_tag="PK960_01875"
                     /inference="COORDINATES: protein motif:HMM:NF040257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HPD07950.1"
                     /translation="MDKKAEQHTIRAIQYGCGKMGCLIMKYLYEKGVEIVGAIDNNSE
                     LIGKDIGDLIGVEQKLGITIHNDPEEVFTANPADICIVAINGLLTEIYPLLTIPLKYG
                     VNTITTCSEAVFPWTTSPEFAKRLDALAKKNYCTLTASGYQDIFWGHLITTLAGGSQK
                     INAIGGTTFYNLEEYGLSMAQSCGAGFTIDEFNKKIGQNNLLPSPIWNAAEWLAASLG
                     WTVKNISQKLVPIIADQSTISSVLNKTIPPGDCLGLTASINLETNDGPVIQIQHSSQV
                     SNPQVLNISEWIIRGESNTRLQLEQISTIKTACATIVNRIPEVMMANPGYMTTHNWPY
                     VSYHEHPLANYLPLK"
     gene            complement(5569..5639)
                     /locus_tag="PK960_01880"
     tRNA            complement(5569..5639)
                     /locus_tag="PK960_01880"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5605..5607),aa:Gln,seq:ttg)
     gene            5695..6777
                     /locus_tag="PK960_01885"
     CDS             5695..6777
                     /locus_tag="PK960_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPD07951.1"
                     /translation="MKTKRFILCLILPLIVIVCPARPLLAESIKVDEFAEETATVVAI
                     RKVLPAVVSITVVESVAQNINGVITTKKIVVGSGTGFIISSDGLILTNRHVVSPKEQG
                     EYKIRFQSGKTYYAQFIGVDPLNDLAILKIFDKNLPTIQLGDSDKLSLGSTVIAIGNA
                     LGRYQNSATKGIVSGIGRNLYASDDEGNETSLTNVIQTDAQINPGNSGGPLINLKGEV
                     VGINVAIDTSGNAIGFAIPINDAKPVIASVKQTGRIIRPRLGVSYIMLNEDLANENNL
                     PVTQGAWLRDSQDQPAIMPNSPAQKAGLQANDIILEINAQPLNEQLSLTQKIQQYRPG
                     QTIGLRVLRGGRQLIIKVTLDEFPQN"
     gene            6787..7755
                     /locus_tag="PK960_01890"
     CDS             6787..7755
                     /locus_tag="PK960_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPD07952.1"
                     /translation="MSQQFDLVTIGGATEDLFLTIDNYHLMDNSQDILAQKLLAIEYG
                     AKILAKESFLSYGGGATNVAVAVSRYNLKSAVIAAIGDDQRGGNILKNLKSHAINSHY
                     IQIIADQQSAMSVMLIGRDGEHSAICYRGANDLITIDFNTIAALNTPWLYISSLGGHS
                     WYKNLINIFSSNKLIAWNPGQTQLIAGVAKLRKFLNKTAIFIVNHDEALQLVSTDNSL
                     RPQQMKVYNQNIELLIEKLKSYGPQLVIITKDKDGAWSYDGKRLIHQPAYLTKNIVDT
                     TGVGDAFSGTFIANFIITHNIRQSLRVAVRQSAIILKVRGAQSWPA"
     gene            7761..8633
                     /gene="mutM"
                     /locus_tag="PK960_01895"
     CDS             7761..8633
                     /gene="mutM"
                     /locus_tag="PK960_01895"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HPD07953.1"
                     /translation="MPELPEVETIVRDLRELIVNKCIKEIIATDKQFSTFNEGRKRQL
                     LIGQKILDISRRGKHVAIKLTNNYYLVIHLKMTGQLIWVKGTKWLAEGHPTNDLATTV
                     QPTWPNNTTRLIINLSDNATLFFNDSRRFGWVRLMSASEWQSYQNKLGVEPLSKNFTN
                     EFLLTLLAQRPHSRIKPLLLNQKYIAGLGNIYTDEALFRAGIRPDRLAGQISPTEVNK
                     LASAIQTVLTEAISHRGTTFSDYRDGYGQKGNFVSYLQVYGRDGQQCRHCGQTIQKIT
                     LAGRGTHFCPCCQH"
     gene            complement(8630..9055)
                     /locus_tag="PK960_01900"
     CDS             complement(8630..9055)
                     /locus_tag="PK960_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HPD07954.1"
                     /translation="MKILIIPLVAALVAQVSKVFIKGNHQKLNWSILWAYSGMPSGHA
                     AITSALTTIIGLEKGITYPGFAIALVFTLLTLRDATGVRRQLGNQGGVINKLVDDLDD
                     DNYLDRRYPHLTEKIGHTPLQTVAGVLLGIIIAVIGYFI"
     gene            9140..10228
                     /locus_tag="PK960_01905"
     CDS             9140..10228
                     /locus_tag="PK960_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPD07955.1"
                     /translation="MKIVIRAGGLGVRLWPFSRQNNPKQFQALLGNKTMIRTTYERVA
                     RLLNRPEDLFISINELMVQRLKLEIPEVKLSNIIVETECRNTGPAMCLEVCYLQKYCQ
                     PNDIIASIPSDDYISNNKAFADLLRSSEQFLRQNPDYILTPATKPTHVNTGYSYLKAG
                     QNLQNVGHEVIFTVSDVVEKPDEDYCQQLINSGVYYCHTGMYVWRLQTIADLFAKFQP
                     EMYKICQQIADLLISYGNNEQIRQLYGQLEKVSIESAITNKVEKLAMSVSDQIGWSDL
                     GKWPVLYDILSDGAGSNVIKGQVVSKDSKNNLVFCNLVDKLVVINDINNLVIVDTGDV
                     LFISSLKKADDTKEIIEDLKQRDLQKYI"
     gene            10233..11132
                     /gene="mltG"
                     /locus_tag="PK960_01910"
     CDS             10233..11132
                     /gene="mltG"
                     /locus_tag="PK960_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPD07956.1"
                     /translation="MQMSDGNKKRPKWPIWCLSVIVLIILCWGLLYISNRLKARQERL
                     KYWQEQAAEQADRTITIIEGWNSEQIAAKLAENNLGFTAKQFLQIAGEPRQDYRLLPT
                     SKWPQDFSDRYDFLKDKPKYFGLEGYLFPDTYRFSPSSTPEKAIMRFLDNFDRQLTPT
                     MRQDIERQGKTIYEIITMASILEKEVRNETDMKLVADIFWRRLKNGQRLESCATLAYA
                     LGKNKPQYSYEETRLASPYNTYINDGLPPGPICNPSLKAIRAAIYPTPNDYNFFLTRL
                     DNGQTIFSHTFEEHELNKAKYLQ"
     gene            complement(11174..11614)
                     /locus_tag="PK960_01915"
     CDS             complement(11174..11614)
                     /locus_tag="PK960_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD07957.1"
                     /translation="MFFKKEEKMEQKKQLEILVVDDDEQNIGLLSRLFNKVLGNDVEI
                     IVAKDEISATTRCGIADLVIVIVECSLPTSISPEQKSIRFKGVDIIKSIRGKRPELIV
                     VAMSSCDKMRQEAKKSGANFFINTIYDNWQLENIIRAIVSQTHH"
     gene            complement(11647..12687)
                     /locus_tag="PK960_01920"
     CDS             complement(11647..12687)
                     /locus_tag="PK960_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HPD07958.1"
                     /translation="MQIIFKYHHCPGFARLYDYANKYNHMVTTEAQQRIKILDFWRNY
                     GLKATRDAYSVSRSTLYLWQKILREKGIEGLSPGSQARKNNNCRLVDSLLVNEIRRLR
                     LEVCPNMGKAKIKIFLDQFSEANGLTKLSESTIGRIIKDKKIYHHRQKITHYGKIKLV
                     VRHKKLRKPKDLITTRPGELVEIDTIVRFINGLKRYIVTAVDTYGRPAFAWCYTRASS
                     ANAKDFFQKLQMVLPFVAIAVQTDNGSEFHQHFMNYLDKQNVTHYWNYPGRPYRNGHI
                     EKFNRTIQEEFIDDHEAWLDNTREFNVKLIDWLIWYNTKRPHWSLQLQSPFQFLLNNG
                     YLSEMRWTDTII"
     gene            12815..>14059
                     /locus_tag="PK960_01925"
     CDS             12815..>14059
                     /locus_tag="PK960_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07959.1"
                     /translation="MFHENKQDIISSQNEQPEISNEPETTENPPEALYSQDDAQQLIE
                     KGEVWRLAKELKNYQDLNENIAEQLLDKGYCREVLDNLDKFRDLEHSKILKCLIDDGH
                     FEELIDYMDKLSDIPHQQIAQELINQGKIELLAQKLEKFSGLDENIAEQLIKENYYDQ
                     VLDNLRAFSNLSLDFAQKLIKGGGEVKVIDNLESFSPEAHNDIAHLLLEKGYGYLILH
                     NWEKFSKVNFSDEDYETIANGIIERNDYQSNKELGENIDKFPRSLHKKIAQQLIDSNN
                     GQVVAKNLEKFSGLNHRKIAQKLIDSGQGNVVAENLEKFSGLDENIAEQLIKVDHYYL
                     VRKGLKAFSNLSLDFAQKLIKEGHGVDVGDNLECFPPEAHNDIAHQLLKEGYDLYLLK
                     NSKKFSKVNFSDEDYETIANGII"
BASE COUNT         4579 a         2477 c         2570 g         4433 t
ORIGIN      
        1 agctaaaaga ttagctagac aatttttata attacaacag cattaaaact atgcctcaac
       61 accaaaaatg gtggagtaaa ttacagcacc ctttgtttat tactattgtt agttgtatta
      121 ttgtaatttt acttatattg gggttactta aaaatatccg tcaattattt aatgcccaca
      181 gagaaattaa tcagataaag acagaaattg acaacttgga caaaaaaaat acagaaatta
      241 gttatctaat taattatctg caatccaaag agtttttaga agaacaggca cggctgaatt
      301 ttggcctgca aaagcccaat gaacagataa tagtagttaa gcaatcggct actgatacaa
      361 ctactaattt agtaaatagc ccagtcttta atgtaccagc gccacctcag cctaaactag
      421 cagttagttc taatcctaaa aaatggtttg attatttttt tgctccacaa taataaatat
      481 tttgagccca tggtcggatt cgaaccgacg acctttccct tacgaaggga ttgctctacc
      541 agctaagcta cacgggcggg agcgggtgag cggagttgaa ccgccatctt cagcttggga
      601 agctgacata ataaccgtta tacgacaccc gctaaataaa tttggtcact accttaaaat
      661 atgttataat gccattataa aaagaaaagt tttacttcgt caactttatt tatgtcttca
      721 tctcataata tcatcttttt attaaaacat cactttttgt ttttatctgt tattataatt
      781 agtattttac ccggagttgt agttgctgat agttcagtaa gttccacttc gagtgttatc
      841 ggccgagcta gttggtataa ttttcgccct ggactatttg ccgcttctaa tatttattcc
      901 ccaggacaaa aagttagggt ttatcatgat gcttctggta aatatgtcga tgtagtcatt
      961 aatgactatg gtcctgatcc cacaatacac cctgataggg taattgattt acaaaaggaa
     1021 gcctttgctc aactagctcc tttaggcgct ggggttatta ctgttagatt agagccttta
     1081 ccgcaaggag aaaatttggc catcatttct caaccggttt cttctttggc ggtagctaat
     1141 gcttctacta gtcctaaagt tgtaagtaat caaacaagcc gtgatttatc taaagaacaa
     1201 gcagctctaa ctcaatttac taaactatat ggtcgcttgc ctaaggactc tgtagattgg
     1261 cagattatta atgatattgc ttataataat ttaaaaaggg atctaaataa agaaaaatta
     1321 gccctaactc aatttactaa actatatggt cgcttgccta aggactctgt agattggcag
     1381 attattaagg atattgctta ttaccaaaaa ttacccaaga ccaataataa ctttaaagca
     1441 gtcaatatct ctaacaaaga ggcagcagcc gctattgtcc tagacggaga tagtggtcaa
     1501 gttttatggg aaaaaaatgc tacccaggtt ttacctatcg ctagtctaac taagttggtc
     1561 gctgttaaaa catttttaga tcttaagcct gaccttgata aggtcgttac ttatagtcaa
     1621 gaagatgaaa aagaaatagg aaaatattgt accccatccg aagcagcgat ggtaaattta
     1681 caagatggtg accaggtaaa aatacgtgat ttagtttatt cagcgcttat aggctcagct
     1741 aacaatgcca tagaatcact ggtgagatta agtggtctaa cccgtgacga gtttataaac
     1801 aaaatgaatg ctaatgccaa aaaatggggc gccacttcta ctaaatttgt tgagcccacc
     1861 ggtttatcgc cggataacgt cagtacagct aaagattatg ccttaattct taaaaatatt
     1921 agccaggatg ctactattaa aaagattagt acaacagcta gctatcaatt cagcactatc
     1981 aataccaagc aaaggcatac tattaaaaat actaacaaac tcttgagaga aggcaacttt
     2041 tctttagaga catctaagac cggttatctt acagaggctg gtcattgttt agctatggcg
     2101 gctcagcaac aaaacactaa acaacatttt attgttgtaa ccctaggttc accaacttgg
     2161 tctaagagct atgaagtagc tagcagatta ttaactaccg ccctcaatta gtaaaccatt
     2221 atgattcgat ttatcaatgc tagtaaattt tatccgcccc aaactaaggc tttagataac
     2281 ctgagtttgc atatctatcc cggtgaattt gtttctattg ttggtcaatc tggcagtggc
     2341 aaaactacta tagtaaaatg cttaattggt gaagaaaaat tagacgaagg ccgagttata
     2401 gtcggtgatt gggacataac tcatatttct aatcgagaaa ttccttatct cagacggcag
     2461 attggtgtca tttttcaaga ttttaaatta ttaccaaaga aaacactagc tgaaaatgtg
     2521 gcttttgctc tacaggtttg cgggaaatca ccaaaaaaga ttaaagctat tgtgcctagt
     2581 gtcatgaaaa ttgttggtct aactgaacaa atgaaccgct atcccaatga agtttcgggc
     2641 ggggaacagc aacgagcagc tattgctcga gctttagtct atcagccaaa aattttagta
     2701 gctgatgaac caactggtaa tctagactct attaatgccg aagagattgt caatttactc
     2761 ttaaaaatta atgatctagg cacaacggtc attttagtaa ctcataatcg agaaatagtt
     2821 aataacctta atcgacgagt tatttctatt agcgatggcc gtttagtcaa tgatcaggct
     2881 aggggtaaat atattattta atattttatg tttttatctt tagcgcggat aattaaattt
     2941 ggctttcaag atgtgtggcg taatttttgg ctatcactag ccactattat gattttagtc
     3001 ctcacgttat tttcggtgga tttattgcta gccctaagaa ttattggcca agcggcagtg
     3061 gataatctaa aaaatcgagt tgatgtatca atttatttaa agcagggtgt acctgaagat
     3121 aagattttaa atcttaaagc ccggctggcc aatttaaata atgttcaaga agtaaattat
     3181 atttcccaaa gcgcagcgtt agaatctttt caaaggcgtc atcaatccga cccagagatt
     3241 ctccaagccc ttcaagaact agaccaaaat ccactggctc ccacactaac agtaaaacct
     3301 aaagatcctg accattatga agagcttatt gaagaacttg accagattga ggatccgatt
     3361 attgaatcac ggaattttca agataataag aaattattag ataagattaa ctatattact
     3421 gataaaatta acgatgttgc tataattatt agttccatct ttttgctaat tatgatttta
     3481 gtaatgttta attcggtgcg gctagctatt tatagtcatc ggcaagaaat agtaattatg
     3541 aagttggttg gcgcttctaa tgcttttatc aaggcacctt ttctcttctc tagtttaatt
     3601 tataccttag tgggtgtagt tctcacagca gtcatttttt atgcttttct tactgttctg
     3661 cagccatatc tagatacttt tttctttggc tataatattg atttaataac ctattttaat
     3721 agtcattggt tacaaattat tggtttgcag tttgccggcg ccgctatggt taacctacta
     3781 gctagctata ttgcagttgg caaatattct aagatttaaa attcatgtta agtcaaaaac
     3841 aagaaaaatt gattaaatct ttgcacacta ataaaggaag aaatgaaacc aatctatggt
     3901 tagtagaggg ggctaaatgt ttagagttag ctggctcagc tgttcaattt aaatttggcc
     3961 ccaaagacag tcatcatttt gcccaattaa ctactactga tactccaccg actatagccg
     4021 gtctagccat accgcctaag tttcagcttg atgatattac tgcccgaaga acaattctcg
     4081 tcctagatca tcttcaggac cctggtaatg ttggcacaat tattcggttg gctggagctt
     4141 ttgatgctag tcttatactt tgcgagtgtg ttgaccctgg taatcctaaa gttattcgtt
     4201 cttcggccgg tttggtattt gccacgcctt ggataccctt accgccccgt atagccctaa
     4261 aatggttaag tgaagttaac tatccaattt atcgtctaga aaataaggca ggagccaaag
     4321 aattaaatat taggactcat aaatttcttt ctgccgataa aataataatt attgttggct
     4381 cagaaggtca aggaattaag cttaaaattc caggacaaag agtggccatt aaacataacc
     4441 ccacagtcga ttcacttaat gtagctgccg ctgtagcgat tgccttattt ttaagatatc
     4501 aaaagaataa taaataatat ggataaaaaa gccgaacaac ataccattag ggctattcag
     4561 tatggctgtg gtaagatggg ctgtctaatt atgaagtatc tctatgaaaa aggagtagag
     4621 attgttggcg ctattgataa caattctgaa ttaattggta aagatatagg ggatttaatt
     4681 ggggttgaac aaaaactggg tattacgatt cataatgatc ctgaagaggt ttttactgcc
     4741 aatccagccg atatttgtat tgtcgctatt aatggtttat taactgaaat ttatcccttg
     4801 cttactattc ctttaaaata tggggtaaat actattacta cttgtagtga agcagttttt
     4861 ccttggacca catcacctga gtttgctaaa cgattagatg cattggctaa aaaaaattat
     4921 tgcaccctca cggcgagtgg ttaccaggat attttttggg gacatttaat tactaccctg
     4981 gctggaggta gccaaaaaat taatgctatt ggtggaacaa ctttttataa ccttgaagaa
     5041 tacggtctat ctatggctca aagttgtggt gctggtttta ccattgacga atttaataaa
     5101 aaaattggtc aaaataatct tttaccatca cccatttgga atgctgctga atggctagca
     5161 gcttctctgg gttggacggt taaaaatatt agtcaaaaat tagtacctat tattgcggac
     5221 caatcaacta ttagctcggt tttaaataaa acaataccgc ccggtgactg tctaggccta
     5281 accgccagta tcaatttaga aactaatgat ggtcctgtta ttcaaatcca acacagtagt
     5341 caagttagca atccccaagt acttaatatt agtgagtgga taattagagg agaatctaat
     5401 accagactgc agctcgaaca aatatcaaca attaaaacgg cctgcgctac cattgttaat
     5461 cgtatacctg aagtaatgat ggctaatcct ggctatatga ctactcataa ttggccttat
     5521 gtcagttatc atgagcatcc actggcaaac tatttacccc taaaatagct cggggacaag
     5581 gattcgaacc tcgattaatg gctccaaagg ccactgtcct accattagac gatccccgaa
     5641 aacttttcta taataactta atcattataa cttgtcaaaa ataattaaaa attcatgaaa
     5701 actaaaagat ttattttatg tcttatacta cccctaatag tcattgtctg tccagctaga
     5761 ccattattag cggagagtat aaaagtagat gaatttgccg aagaaacagc aacggttgtt
     5821 gctattcgta aggttctacc ggcggtagtg agtattacgg tagttgaaag cgtggctcag
     5881 aatattaacg gagtaataac cactaaaaaa attgtggtgg gctcgggcac tggtttcatt
     5941 atttccagtg atggacttat tttaactaat cgccatgtag ttagcccaaa agagcaaggt
     6001 gaatataaga ttcgtttcca atctggtaaa acttattatg ctcaatttat cggtgttgac
     6061 ccacttaatg atttagctat tctaaaaatt tttgataaaa atctaccaac tatccagtta
     6121 ggtgatagtg ataaattgag tttaggttca actgttatcg ctataggtaa tgccttgggt
     6181 cgttaccaaa atagtgcaac taaaggtata gtaagtggca ttggtcgtaa tctttatgct
     6241 agtgatgatg aaggtaacga gactagttta accaacgtta ttcaaactga tgctcaaatt
     6301 aatcctggta attcaggggg acctttaatt aatctaaaag gagaagtcgt gggtattaat
     6361 gttgctattg atactagtgg caatgctatt ggttttgcta ttcccattaa tgatgccaag
     6421 cctgttattg ccagtgttaa gcaaactggc cgcattatta ggccaagatt gggggttagc
     6481 tatattatgc ttaacgaaga tttagctaat gagaataatc tgccagtgac tcagggtgct
     6541 tggctgaggg atagccaaga tcaaccagcc atcatgccta attcaccggc ccaaaaagct
     6601 ggacttcagg ctaacgatat tattttagaa attaatgctc agccacttaa tgaacagctt
     6661 agtcttactc agaaaattca acaatatcgc cctggtcaaa caattggtct gcgggttttg
     6721 cgaggcggac gacaattaat tatcaaagtc actttagatg aatttcctca aaattaagat
     6781 cataatatga gtcaacaatt tgatttagtt actatcggtg gagccactga agatttattt
     6841 ttaaccattg ataattatca cttaatggat aattctcaag atattttagc tcaaaaatta
     6901 ttggctattg agtatggagc caaaatatta gccaaggaaa gttttttaag ctatgggggt
     6961 ggagcaacca acgtagcagt agccgtcagt cgttataact taaaatcagc tgtcatagca
     7021 gctattggag atgaccagag aggaggtaat atattaaaaa atttaaagtc tcatgctatt
     7081 aacagccatt atattcaaat aattgccgac cagcaatcag ccatgtcggt aatgctaatt
     7141 ggtcgagatg gtgaacatag tgctatttgt tatcgcggtg ctaatgatct aataaccata
     7201 gattttaata ctatagccgc cttaaatacc ccctggttat atatttcttc tctaggcggt
     7261 catagttggt acaaaaacct aattaatatc tttagttcta ataaattaat cgcttggaat
     7321 ccgggacaaa ctcagcttat agccggtgtt gctaaactta gaaaatttct aaataaaact
     7381 gcaattttca tagtaaatca tgacgaagct ttacaattgg tttctaccga taattcttta
     7441 cggccacaac agatgaaagt ttataatcaa aatattgaat tattaataga aaaactgaaa
     7501 agttatggcc cccaattagt aattattacc aaagataaag atggtgcgtg gtcctatgat
     7561 gggaagaggc taattcatca gccggcctat ttaactaaaa atattgtaga tactaccgga
     7621 gtaggtgatg cttttagcgg tacatttatt gctaatttta ttattactca taatattcgc
     7681 cagtcactac gggttgcggt tcgacagtcg gctataattt taaaggttag gggagcacag
     7741 tcatggcccg cataaaaagt atgcctgaat taccagaagt agaaacaatc gtgagggacc
     7801 ttagagaatt aatagtaaat aaatgtatta aagaaattat tgctaccgat aaacaattta
     7861 gtacctttaa tgagggtcgt aaacggcaat tacttattgg acagaaaata ttagatattt
     7921 caagacgagg taagcatgtg gctataaaat taactaataa ctattatcta gttattcatt
     7981 taaaaatgac tggccaatta atttgggtta agggtaccaa gtggttagct gagggccatc
     8041 ccactaatga cttagccacc actgttcaac caacttggcc taacaataca actcgtttaa
     8101 taataaatct ttccgataat gccactctat tttttaatga ttcaagaaga ttcggctggg
     8161 taagattaat gagcgcatcg gaatggcaaa gttatcaaaa taaattaggt gtcgaaccac
     8221 taagtaaaaa ttttactaat gaatttttat taactctttt agcccaaagg ccgcattctc
     8281 gtattaaacc cttactttta aatcaaaaat atatagccgg gttgggtaat atttatactg
     8341 atgaggcttt atttagggcc ggtattaggc cagaccgctt ggcaggtcag atatctccaa
     8401 ctgaagtcaa taaactagca tcagctatcc aaacagtatt aactgaagct attagtcatc
     8461 gaggaaccac ttttagtgat tatcgcgatg gttatggtca aaaaggcaat tttgtaagct
     8521 atttacaggt ttatggacga gatggtcagc aatgccgtca ttgtggtcag actattcaga
     8581 aaattactct tgccggtcga ggtactcatt tctgtccttg ttgtcaacat tagataaaat
     8641 aaccaatgac ggctattata attcccaata aaactccagc tacagtttgg agtggagtat
     8701 gaccaatttt ctctgtaaga tgcggataac gtctgtctaa atagttatca tcatctaggt
     8761 cgtccaccag cttgttaatt actcctcctt ggttacctaa ttgacggcgt acaccagtag
     8821 catcacgtaa agtaagtaag gtaaacacta aggcaatagc aaagccaggg taggttatgc
     8881 ccttttctaa tccaataatg gtggttaaag cactggtgat agcagcatga cctgatggca
     8941 ttcccgaata agcccaaaga atagaccaat ttaatttttg atgatttccc ttaataaaga
     9001 ctttgcttac ttgggcaacc agggctgcca ctaacggtat aattagtatt ttcatatatt
     9061 tttattaatt gattcattat agcaaaaaat gaattaatcg gctacaataa taagagctta
     9121 attattaatt attaagatta tgaaaatagt tattcgggct ggcggtctag gagttagatt
     9181 atggccgttt agtcgtcaaa ataatcccaa gcagtttcaa gctttattag gcaataaaac
     9241 aatgattaga accacttacg aaagagtggc caggttatta aaccgaccag aggatttatt
     9301 tatttccatt aatgaactca tggttcaacg gcttaaacta gaaattcctg aggttaaatt
     9361 aagcaatatc attgtagaga cagaatgtcg caatactggc ccggctatgt gtttagaagt
     9421 ttgctattta caaaagtact gtcagccgaa tgatattatt gcttcaatac cctctgatga
     9481 ctatatttcc aataataaag cttttgctga tttattaagg tctagtgaac aatttttacg
     9541 tcaaaaccct gactatattt taaccccagc tactaagcct acccatgtca atactggcta
     9601 tagttatctt aaggctggac aaaatttaca gaatgtcggg catgaagtta ttttcacagt
     9661 tagtgacgta gtagaaaaac ccgacgaaga ttactgtcag caacttatta attccggagt
     9721 atattattgt catacaggta tgtatgtctg gcgcttacaa actattgccg acctctttgc
     9781 taaattccag ccagaaatgt ataaaatttg ccagcaaata gctgatttat taattagcta
     9841 tggcaacaat gaacagattc gccaactata tggtcaatta gaaaaagtat ccattgagtc
     9901 cgccattact aacaaagtag aaaaattggc catgagtgtt tctgatcaaa taggctggag
     9961 tgatttgggt aagtggccag ttttatatga tattttatct gatggtgccg gttccaacgt
    10021 cattaaggga caagtagtat ccaaagattc taaaaataat ctagtttttt gtaatttagt
    10081 ggataaatta gtggtcatta atgatataaa caatcttgtt attgttgata ctggtgatgt
    10141 actctttatc tcctcgctta aaaaagctga tgacaccaaa gaaattatcg aggacttaaa
    10201 acaacgtgac ttacaaaaat atatttaatc ttatgcagat gagtgatggc aataaaaaac
    10261 gtcccaaatg gcctatttgg tgtctatccg ttatagtttt gattatttta tgctggggat
    10321 tattatatat tagtaatcgc cttaaagctc gccaggagcg tttaaaatat tggcaggagc
    10381 aagcagcaga acaagctgat aggactatta cgataattga aggttggaat agtgaacaaa
    10441 ttgctgctaa attagccgaa aataatttgg gttttacagc taaacaattt ttacaaatag
    10501 ctggtgagcc acgtcaagat tatcggctac tgccaactag caaatggcca caagattttt
    10561 ctgaccgcta tgactttctc aaagataaac ctaaatattt tggcttagag ggttatcttt
    10621 tccctgatac ctatcgtttt agcccttcat caactccaga aaaggcaatt atgcgctttt
    10681 tggataattt tgaccgtcaa ttaactccta ctatgcgtca agatattgaa cggcagggga
    10741 aaactattta tgaaataatt actatggctt ctattttaga aaaagaagtc cgtaatgaaa
    10801 ccgatatgaa attggtcgcc gatatttttt ggcgtcgcct taaaaatggt caacgactgg
    10861 agtcttgtgc cactttagct tatgctttgg gaaaaaataa gccccaatat tcttatgaag
    10921 aaactcgatt agcttcacca tataatacct atattaacga tggcctacca ccgggcccga
    10981 tttgtaatcc cagtcttaaa gccattcggg cggccattta tcctaccccc aatgattata
    11041 acttctttct tactcgatta gataatggac agactatttt tagccatacc tttgaagaac
    11101 atgaattaaa taaagctaaa tatttgcaat aaaaagctcg cttaggtagc gagctcaaaa
    11161 agaattttta acgttaatgg tgggtctggg aaacaatggc tcgaataata ttttctagtt
    11221 gccaattatc atagatagtg ttaataaaaa agttagcccc actctttttg gcttcttggc
    11281 gcatcttgtc gcaactactc atagccacta cgattaattc gggccttttt ccccttattg
    11341 acttaattat atctacccct ttaaagcgga tacttttttg ttcgggggag atagaagttg
    11401 gtaaactaca ttcaacgata acgataacta agtcggcaat gccgcaacgg gttgtggccg
    11461 atatttcatc tttggccact attatctcca cgtcattgcc caaaactttg ttaaatagac
    11521 gacttaacaa gccaatattt tgttcatcgt cgtccacaac tagtatttct aattgctttt
    11581 tctgttccat tttttcctcc tttttaaaga acattaaatt atgtttacaa taaaattaat
    11641 aatttgtcaa ataattgtat cagtccacct catttcggac aagtacccgt tatttaataa
    11701 aaattgaaag ggcgattgga gctgaagact ccagtgtggt cttttggtat tataccaaat
    11761 gagccaatct attaatttaa cattaaattc tctagtatta tctaaccaag cctcatggtc
    11821 atcaatgaac tcttcttgga tagtacgatt aaacttttca atatgtccat tacgatatgg
    11881 tctaccagga taattccagt aatgggtaac attttgttta tctagataat tcataaaatg
    11941 ttggtggaat tcactaccat tgtcagtctg tactgctatg gcgacaaagg gcaagaccat
    12001 ttgtagcttc tgaaagaagt ctttagcatt agcgctgcta gctctagtat aacaccaggc
    12061 aaaagccgga cggccatagg tatctacagc cgttacgata tatcttttta acccattaat
    12121 aaatctaact atagtatcta tctctactaa ctctccgggt ctagtagtta ttaaatcttt
    12181 aggttttctt agttttttat gtctaaccac taatttaatt ttaccataat gagttatctt
    12241 ttgtcggtga tgatatatct tcttgtcttt aatgatgcga cctatggtag attcagagag
    12301 tttagtgagg ccattagcct cactaaactg atctaaaaag atcttgattt tagctttacc
    12361 catattagga caaacttcta gccttaatcg tctaatttca ttaactagta aagaatctac
    12421 tagacgacaa ttattatttt ttctagcttg gctacctgga cttagacctt ctataccctt
    12481 ctctcttaat atcttttgcc atagatacag agtggaccgt gagacgctgt aggcatccct
    12541 ggtagcctta agaccataat tacgccaaaa gtctaatatc tttatccttt gttgagcctc
    12601 tgtagtaacc atatgattat atttattagc ataatcatat agtctagcaa accctgggca
    12661 atgatgatac ttaaatatta tttgcatata ttttgtcctc ctgcggagtc caaaatgtga
    12721 gtgaacttat tcaataatct aattattacc aattcttgac ttaatgatag aaaattatta
    12781 taataatatc ataatataat aaataaacaa ccatatgttt cacgaaaaca aacaagatat
    12841 catctcatct caaaatgagc aaccggaaat cagtaatgag ccagagacaa ctgagaatcc
    12901 accagaggcc ctatattctc aagatgatgc tcaacaatta attgagaaag gggaagtttg
    12961 gcggttggcc aaagaattaa agaattatca ggatttgaat gaaaatattg ccgagcaact
    13021 attagataaa ggatattgta gagaggtctt agacaactta gacaaatttc gtgatctgga
    13081 gcatagcaaa attcttaaat gcttaataga tgatggtcat tttgaagaat taatagatta
    13141 tatggataaa ttatccgaca tacctcacca gcaaattgct caagaactta tcaaccaagg
    13201 caagatagaa ttattagccc aaaagcttga aaaattttct ggtctagacg aaaatattgc
    13261 cgaacaactt ataaaagaaa attattatga tcaggtacta gataacctta gggccttctc
    13321 taatttaagt ttagatttcg ctcaaaaact aattaaggga ggtggtgagg taaaggtgat
    13381 cgataaccta gagagttttt ctcccgaggc ccataatgac attgctcacc tcctattaga
    13441 aaagggttat ggatacttaa tactacataa ctgggaaaaa ttttctaagg taaatttttc
    13501 tgatgaagat tatgaaacta ttgctaatgg gattatagag cgtaacgatt atcagtccaa
    13561 caaggaatta ggcgaaaata ttgacaaatt tccccgctca ctccataaaa agattgctca
    13621 acaattaatt gacagtaaca atggtcaggt ggtagcaaag aatttagaaa aattttccgg
    13681 tctaaatcat cggaagattg ctcaaaaatt aattgacagt ggtcaaggta atgtggtagc
    13741 agagaattta gaaaaatttt ccggtctaga cgaaaatatt gctgaacaac ttataaaagt
    13801 agatcattat tatctggtac gaaaggggct taaggctttc tctaatttaa gtctagattt
    13861 cgctcaaaaa ttaattaagg aaggtcatgg ggtagatgtg ggcgataacc tagagtgttt
    13921 tcctcccgag gcccataatg acattgctca ccaattatta aaagaaggtt atgatttata
    13981 tctgctaaaa aactcgaaaa aattttctaa ggtaaatttt tctgatgaag attatgaaac
    14041 tattgctaat gggattata
//
LOCUS       DAOOIS010000012        11781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_4999_length_11781_cov_46.768463, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000012 DAOOIS010000000
VERSION     DAOOIS010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11781
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4999_length_11781_cov_46.768463"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="PK960_01930"
     CDS             <1..1061
                     /locus_tag="PK960_01930"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPD07960.1"
                     /translation="SIITGVWVGNNDNKSMSKGADGSVVAAPIWQNYMNRILTGTPVE
                     TFKKPEIEDTGKPVLDGVIPTTTVVKVDKVSGLLATSSTPPDMIREDRYWQGHCILYY
                     VDRENPRGPAPTNPEADPQFAPWEAGVKSWAEKQKLNLGEPPKDSDNLHIPENQPDLT
                     IISPSAGQQITDHTLLVNATATAPRGVKTFNLYINGFLLKTINADHLDEEISLDFLPD
                     GQHKLAIEACDDVKNCTKHELTIVLGGGSNVGNLTMTWLSPDDGLTYRAADFPVALQL
                     KITNFKLAHKIKVYYKIKGSSPQLITVVQPADNGLADINFPKPAEGNGTYVITAEAEL
                     WTGETITSPSLTLLVRGL"
     gene            complement(1056..1898)
                     /locus_tag="PK960_01935"
     CDS             complement(1056..1898)
                     /locus_tag="PK960_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPD07961.1"
                     /translation="MKQQLNIGLDIGGSKILGLLYDHNNNKAYAALRQPIIKDSLPAL
                     VRQIHSEIERLIEAAQKTGNIPTGIGIGIPGVIKNGQIINCPNLSVLNNRNLNKLLAK
                     QFPDKKILSDNDVNCFLRAYLVKHPLLKNTTFAVVALGTGIGGAIAINGNNIIPELGI
                     SSEIGHMIINSQSGADLEHYYHKIMGHPAGRLFTAAQAGDFLAQQKVEKFAKILGLAM
                     VNLNNLINPKVIILTGGVSRYYKTYLPLVKQIINEQSLTPNRPKWIVGQDQKAAAIGA
                     SLLL"
     gene            complement(1895..2755)
                     /locus_tag="PK960_01940"
     CDS             complement(1895..2755)
                     /locus_tag="PK960_01940"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPD07962.1"
                     /translation="MNLDWQEAYIKMLDELKVKHLRLVAYWPMIEEKEGTYDFSELDW
                     QIKQASQRNVDIILAVGARLPRWPECHYPQWYTQQDKQTQQKKLLDYIQATINRYDAN
                     PAIVAWQIENEPFLIGFGKCSRLNVRLLDQEIALARQNTKKPIVVTDSGELGIWIPAA
                     RRADIFGTSIYRHIYSQPLKHYMTYPLSPNFFRIKTKLTELVSNAKIIVIEMQAEPWG
                     PQPYFTLSEEEKNKTMNMDKLKKMLEYGRQGGFDTFYLWGVEWWYWEKEINGNNEYWE
                     FMKNLFSQSL"
     gene            complement(2911..3858)
                     /locus_tag="PK960_01945"
     CDS             complement(2911..3858)
                     /locus_tag="PK960_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPD07963.1"
                     /translation="MWLLITIIANLLNGVVYLVDKYFLSQKVHSSVSYAFYVGIWSIG
                     NCVFFIFNRHIPTFGWLLLDLLAGLIFMAALYFWYKALHQGETTKVVPIAGSFIPFFT
                     LLLNFLIGRHNLTSQELLALIILIAGTAFVSFNPRDNLWHKWWIKIKKFLQPHKASLR
                     STGRLLFNALLASFIFATYYVMIKYIYDSQGFSNSFVWTRLGSFLGALLLLIPSRNRK
                     LIFARPRRQATIKSWPLFLGIRLTAFIAFAMTNFAISLADVSLVNSLQGLQYIFLLII
                     AFFLAKRYPKLLKQEINRRNIWHKLAGIILINIGLFLLL"
     gene            complement(3862..5034)
                     /locus_tag="PK960_01950"
     CDS             complement(3862..5034)
                     /locus_tag="PK960_01950"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HPD07964.1"
                     /translation="MTSQISCHFPKGFLWGTATSAHQTEGNNLNDWSLWEPGKTVDGS
                     VSGIACDSYHHFREDIGLAKKLNNNAYRFSIEWSRIEPKQGQFNTKALRHYQAVINEC
                     LKQKIEPVVTIHHFTHPLWFSAIGGWENQAAPQIFNNYVLYLAQNITGVKIWCTINEP
                     MLYAWNAYGRGKWPPQKKNLGTWQQVLNNLIKAHCLAYQTLHLINKKCLVGIANNSQY
                     FKPINNNWLNKEINKIAKYIINDYFFKKTAGYEDYIGINYYFYKLIGLGRRPPDNLLR
                     NDLGWPINPYGLYDVLISAKKYNLPIYILENGIADSQDKYRANFIIEHLKMVYQAIQN
                     GCNIKGYFYWSLLDNFEWDKGFVPRFGLYTVDYPSLKRKPRPSSKIYATICKNNGL"
     gene            5156..5614
                     /locus_tag="PK960_01955"
     CDS             5156..5614
                     /locus_tag="PK960_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07965.1"
                     /translation="MKLKHYLLINLGAMIIFWLLWLVILFKVDPLTTNWLGRGLFYLT
                     LFAAIFSTMSLFGFILRFSLIRQQLARQAVITAFRQAALIAIGVVIALWLLSRQLLGW
                     FNLFILVAGLVMLEFFMLSCQSQNTRLSAQKISKHATSKDIISSTFKENH"
     gene            5625..6023
                     /locus_tag="PK960_01960"
     CDS             5625..6023
                     /locus_tag="PK960_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07966.1"
                     /translation="MWNPLKLFHKKTPSLTPSRFPLVVVGQITDIKKHPEADRLSMVT
                     VDVGSQQLNIVCGANNIEIGQFVPVALEGAKLSSGEVITPREIRGQMSLGMLCSAKEL
                     GLGEDDSGIMILDKSKVKQLGQTLDDYLTN"
     gene            6036..6479
                     /locus_tag="PK960_01965"
     CDS             6036..6479
                     /locus_tag="PK960_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07967.1"
                     /translation="MEIFSTSHDLVNLALTIGIIGVSAAIVYFFIVASNFIRELHRTI
                     DELNRQLETISQITESIKGKLSFMVSYWAILEKVGSKVIDLFQNKMFSRIKDKVFHSS
                     TKKHKKSEEENDLDNNQIEVEPKSETKVKTKAKIKKSKKNKTKVS"
     gene            complement(6472..8508)
                     /gene="topA"
                     /locus_tag="PK960_01970"
     CDS             complement(6472..8508)
                     /gene="topA"
                     /locus_tag="PK960_01970"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPD07968.1"
                     /translation="MRLIIVESPTKAKTISHFLTKTDTVESSFGHVRDLPTKKLGVDI
                     ENNFEPEYEVPAKAKKVVAKLKTLAKKADEIILATDEDREGEAIAWHLQQLLKLPANQ
                     KNRIVFHEITESAIKNALKAPRELDYNLINAQQARRILDRLVGYELSPFLWKKVAKNL
                     SAGRVQSVAVRLIVEREREIEKFIKQEYWTITAHLQAHEEKFTAQLVKWQGKTLEKFA
                     LADQQKAEEIKEKLTGEEFVIDKITKEKILKMPPPPFTTSTLQQVANRWLGFSARQTM
                     VLAQQLYEGINLGSEGQVGLITYMRTDALDLSSEFIHQARKVIQDNFGHQFIAKTPRK
                     FINKNKRAQEAHEAIRPTNPQRTPESIKQYLDPRQFRLYELIWQRALASQAAEAQINH
                     LSIDIKANSAIFLATGQTITFPGYLSIYPEQTQEKNLPHLTDKEHLKLLELTTDQHFT
                     QPPARYSDAGLVKQLEKYGIGRPSTYAPIIATIEKRGYVARDNYKRLAPTAIGKIVND
                     LLVKHFPEIVDYQFTANIENNLDDIARGEVAWPKVIADFYWPFHQHLEEKNQELSKKD
                     IMPETPTNETCDKCGAKMVIKIGRYGPFLACSAFPKCRNIKSLKSPSTPKPLPEKYQY
                     LAKEYANETCDKCGAKMVIKIGRYGPFLACSAFPKCRNIKKIQKTTKPNSTNFS"
     gene            complement(8568..8963)
                     /locus_tag="PK960_01975"
     CDS             complement(8568..8963)
                     /locus_tag="PK960_01975"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPD07969.1"
                     /translation="MAKQNNPWTNFGQAVALAYFQKTGYKIIASNWHYHQQKIDLVAQ
                     QRSIIVFIEIKLYLTNKFSYTNKIMGSYQEKQLQIAANYFLERYKINKWRYDFIIISI
                     NGYRGMAKLKHYRQITPSPHYLKKFQSNT"
     gene            complement(9191..9264)
                     /locus_tag="PK960_01980"
     tRNA            complement(9191..9264)
                     /locus_tag="PK960_01980"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9228..9230),aa:Ala,seq:ggc)
     gene            complement(9280..9351)
                     /locus_tag="PK960_01985"
     tRNA            complement(9280..9351)
                     /locus_tag="PK960_01985"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9316..9318),aa:Arg,seq:cct)
     gene            complement(9804..9879)
                     /locus_tag="PK960_01990"
     tRNA            complement(9804..9879)
                     /locus_tag="PK960_01990"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9844..9846),aa:Cys,seq:gca)
     gene            10044..11363
                     /locus_tag="PK960_01995"
     CDS             10044..11363
                     /locus_tag="PK960_01995"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPD07970.1"
                     /translation="MKAFFVDGSLFLLNILLKVKRIIVWLLSRIFKLVGRLFIYWPAT
                     KIYKLYLHLRRRMINRYQQNAWLAVITFLLIALFIVLYNQQESNQLMAAEDLVRGTWL
                     SRLVGDEFNQFNDIVVDNQPPREFIVNNPAIITNNLSTLDLAKSTDLTTVLGMTTDRW
                     AYTLGPVSLPQVNIQGTTSTAPSRAITYYTVKIGDTLSGIAKAFGVSINTIAWQNGLS
                     PNALLRPGDKLTILPVSGVLHKVKAGDSLSKIAKQYGVKTADIIAYNNLSGDKVVIGQ
                     SLIIPGGQPPSTLASQITQAVKSNVQQVFTSAKETAQKSLTSSGSGMTWPTVGHRITQ
                     YYSWRHTGIDIGNKVGTPIYAADSGRVEIAATGWNGGYGTTIVINHGKLKTRYGHLSR
                     LYVKAGENVVKGQVIGEMGSTGRSTGSHLHFEVLSGNVRYNPLNYVH"
     gene            11375..11629
                     /locus_tag="PK960_02000"
     CDS             11375..11629
                     /locus_tag="PK960_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07971.1"
                     /translation="MLHFLGGLAIVVLGAVIILKREWILNNFGRIDFFEEKLSAYGGT
                     RMAYSLIGLIAIFIGFCIAFGLINNFLGWMLGPLLRYQQS"
     gene            11639..11715
                     /locus_tag="PK960_02005"
     tRNA            11639..11715
                     /locus_tag="PK960_02005"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11673..11675,aa:Ala,seq:tgc)
BASE COUNT         3490 a         1994 c         2318 g         3979 t
ORIGIN      
        1 cgtcaattat taccggggtt tgggttggca ataatgacaa taaatcaatg agtaagggtg
       61 ctgacggtag tgtagtcgct gctccaattt ggcaaaatta catgaatcgc attttaactg
      121 gtaccccagt agaaacattt aaaaagccag aaattgaaga taccggcaag cctgttttag
      181 atggagtaat tccgactact actgtagtca aggtggataa agtttctgga ttattagcta
      241 ctagttctac accacccgat atgattcgcg aagaccgtta ttggcaggga cattgtattc
      301 tttattatgt tgatcgtgag aatcctcgcg ggccagcacc cactaaccct gaagctgatc
      361 ctcaatttgc tccctgggaa gcgggggtta agagttgggc cgagaagcag aagcttaatt
      421 taggtgagcc acccaaagat agtgataatt tacatattcc tgaaaatcaa cctgatttaa
      481 cgattattag tccatcagca gggcaacaaa ttactgacca tactttatta gttaatgcta
      541 ctgctactgc acctcgaggg gttaaaactt ttaatcttta tattaatggc tttttattaa
      601 aaaccattaa cgctgaccat ttagatgagg aaattagttt agatttttta cctgatggcc
      661 agcacaaatt agcgatagag gcttgtgatg atgttaaaaa ttgcaccaag catgagctta
      721 ctattgtttt agggggaggt agtaatgttg gtaatttaac aatgacctgg ttatcccctg
      781 atgacggtct tacttatcga gcagctgatt ttccagtggc tcttcagctt aaaattacta
      841 attttaaatt agctcataag attaaggtgt attataaaat taaaggctct tcaccccaac
      901 ttattactgt cgtccagccg gccgataatg gattggctga tattaatttc ccaaagccgg
      961 cagaaggaaa tggtacttat gtaataacag cagaggcaga attgtggacc ggagagacca
     1021 ttactagccc cagtcttact ttattggtga gaggattata ataataaact agctccaata
     1081 gcggccgctt tttgatcttg tcctacaatc cacttgggcc tattgggggt taggctttgt
     1141 tcattaatta tctgttttac tagaggtaga taagttttat agtatcggct aactcctcca
     1201 gttaaaatta ttactttagg attaataaga ttatttagat taaccatggc aagtcctagt
     1261 attttagcaa atttttctac tttttgctgg gccagaaagt cacccgcctg ggcggcagta
     1321 aagagacgac cagccggatg acccattatt ttgtggtaat aatgctctaa atcagctcct
     1381 gactgtgaat taatgatcat atgacctatt tcactgctaa tacctagttc agggataata
     1441 ttatttccat ttatagcgat agctccacca atacctgtgc ccaaggcaac cacagcaaag
     1501 gtggtatttt ttagtaatgg atgtttaact agataagctc ttaagaaaca gttaacgtca
     1561 ttatctgata atattttttt atcagggaat tgcttggcta ggagtttatt taaatttcta
     1621 ttgtttaata cacttaagtt tggacaattt attatctggc catttttaat tactccgggg
     1681 atgccaatcc caataccagt gggaatattt cctgtttttt gagcagcttc aattaaacgt
     1741 tcaatttctg agtgaatttg tcggactagt gctggtaagc tatctttgat aataggctga
     1801 cgaagagcgg cataagcttt gttgttgtta tggtcatata agaggcctag aatttttgag
     1861 cctccaatgt ccaggcctat gtttagttgt tgtttcataa agattgacta aaaagatttt
     1921 tcataaattc ccaatattca ttgttaccat ttatttcttt ttcccaatac caccactcta
     1981 cgccccagag ataaaaagta tcaaaaccgc cttgccggcc atattctagc attttcttta
     2041 atttatccat attcatggtt ttgttttttt cctcttcgga taacgtaaag tagggttggg
     2101 ggccccatgg ctcagcttgc atttcaatga caatgatttt ggcattactt acaagttcag
     2161 ttaatttagt ttttatccta aagaaatttg gagataaggg gtaggtcata tagtgcttaa
     2221 ggggctgact ataaatatgt cgataaatcg aagtaccaaa aatatcagct cgtctagcgg
     2281 ctggtatcca aatacctaat tcaccgctat cggttacgac aattggtttt tttgtgtttt
     2341 gacgggccag agctatttct tggtctaaga gtcttacatt tagcctggaa catttaccga
     2401 agccaattaa aaatggctca ttttctatct gccaggcaac tatagccgga ttggcatcat
     2461 atctattaat tgttgcttga atataatcaa gtaatttttt ttgttgagtt tgcttgtctt
     2521 gctgagtata ccactgtgga tagtgacatt caggccagcg tggtagacga gctccgaccg
     2581 ctaaaattat atccacattt ctttggctgg cttgtttgat ttgccaatct aattcactaa
     2641 agtcgtatgt cccctctttt tcttcaatca ttggccaata agccaccaac cttagatgtt
     2701 taacttttaa ttcatcgagc attttaatgt aagcttcttg ccaatctagg ttcatgtctt
     2761 tcaagacgag tggagaaaaa gtcagaccat atgtaagttc atgtggcgaa taaatagttc
     2821 gtctagttag ccaaatccat aataagccga ggattattag acagcttaaa atgatgataa
     2881 agcgtcgcca atgttttttt attcgtttga tcataatagc agaaagagtc ctatattaat
     2941 taaaataata ccagctaatt tatgccaaat attgcgtcga ttaatctctt gtttaagtaa
     3001 cttgggatag cgtttagcta agaagaaggc aattatcagg agaaatatat attgtaatcc
     3061 ttggaggcta ttaaccaatg agacatcagc tagtgatata gcaaagttag tcatagcaaa
     3121 ggcaataaag gcggttaatc taatacctaa gaataatggc caacttttta tagtagcttg
     3181 gcgtcgtggc cgggcaaaga ttaactttcg gtttctggag ggaattaaca acagtaaagc
     3241 tcctaaaaaa ctaccaagtc tagtccagac gaaagaatta ctaaagccct gggagtcata
     3301 tatgtatttt atcataacgt aataggtagc aaaaataaac gaagctaaga gtgcattaaa
     3361 taaaaggcga ccggtgctac gcaggctagc cttatggggc tgaaggaatt tttttatttt
     3421 gatccaccat ttatgccata agttatcacg gggattaaaa gatacaaatg ccgtgccggc
     3481 aatcaaaatg atgagggcta ataattcttg tgacgttaga ttatgtcgac cgattaaaaa
     3541 atttaacagt aaagtaaaaa atggaataaa agaaccagcg atcggaacaa ccttagtagt
     3601 ttccccttgg tgtagggcct tataccaaaa gtagagagca gccataaaga ttagaccagc
     3661 taaaaggtct aataatagcc agccgaatgt cggtatatgt cgattaaaga taaaaaagac
     3721 acaatttcct atactccaaa tgccaacata aaaagcataa gataccgatg aatgcacctt
     3781 ttgtgataaa aaatatttat ccactaaata aactactcca tttaaaagat tggctataat
     3841 ggtaatcagt agccacatat tttaaaggcc attatttttg caaattgtag cgtatatttt
     3901 actactaggt cgaggctttc gtttaagtga ggggtaatca acggtataga gtccaaatcg
     3961 gggcacaaag cccttatccc attcaaaatt atctagtaaa gaccaataaa aatagccctt
     4021 aatattacaa ccattttgaa tagcctgata gaccattttt agatgttcaa taataaagtt
     4081 tgccctatat ttatcttgag agtcagctat cccattttct aggatatata ttggtaaatt
     4141 atattttttg gctgagatta aaacatcata tagtccatac gggttgatag gccatcctag
     4201 gtcattacgc agtaagttat cagggggacg tcgtcctaag ccaatgagtt tatagaaata
     4261 atagttaatg ccaatatagt cttcatagcc agcagttttt ttgaagaaat aatcattaat
     4321 tatatattta gcaattttat ttatttcttt atttagccag ttattattta ttggtttaaa
     4381 atattggcta ttattagcaa tcccaactag gcatttttta ttaattaaat gtaatgtttg
     4441 gtaggctaga caatgggctt taattaaatt attaagaact tgttgccagg tacctaaatt
     4501 ttttttctgt ggtggccact tgcctcgtcc ataagcattc caggcataga gcattggttc
     4561 attaatagta caccagattt taacgccagt aatattttgg gctaaataga gaacataatt
     4621 attaaatatt tgtggtgcag cttgattttc ccagccccca atagctgaaa accacaaggg
     4681 gtgggtaaag tgatgaatag taacaactgg ttctattttt tgtttgaggc attcattaat
     4741 tactgcttgg tagtgtcgca aggccttggt gttaaattgt ccttgtttgg gttcaattct
     4801 gctccattca atagagaaac ggtaggcatt attattaagt tttttagcta aaccaatgtc
     4861 ttctctaaaa tgatgataag agtcacaagc aatgccagag accgagccat caactgtttt
     4921 gccgggttcc catagggacc agtcattaag attattgcct tcagtctgat gggctgatgt
     4981 agccgtgccc cataggaatc ctttagggaa atggcacgat atttgagaag tcatgtataa
     5041 cctttattta tattaaatat aatggcagta tatcattttt taacactgga gacaataaaa
     5101 tctttaccgt tgtaagtgaa ttatagtata atatagttga tttaatttaa aagtcatgaa
     5161 attaaaacat tatctcttga ttaatttagg agccatgatt attttctggc tgttgtggct
     5221 tgtaatactt tttaaagtag acccattaac tactaattgg ttaggtcgag gtctttttta
     5281 tttaaccctg tttgcagcaa tttttagcac tatgtcttta ttcggtttta ttcttcgctt
     5341 tagtctaatt cgccaacaat tagctcgtca ggcggttatt accgcttttc ggcaggcagc
     5401 gctaattgct attggagtgg ttattgcctt gtggctctta tcacggcagt tacttggctg
     5461 gtttaatctg tttattttag tcgctggttt agtgatgtta gagtttttta tgttaagttg
     5521 ccaatctcaa aatactcgat tatcggccca gaaaatctct aagcatgcca cttctaaaga
     5581 cataatttct tcaactttta aggaaaatca ttaactttta tattatgtgg aatccattaa
     5641 aactttttca taaaaagact ccgtctttaa cacctagccg ttttcctttg gtagtagttg
     5701 gccaaataac tgatataaaa aaacatcctg aggctgaccg attatctatg gttacagtag
     5761 atgttggcag tcagcaatta aatatagttt gcggggctaa taatattgag attggccaat
     5821 ttgtacccgt tgccttagaa ggtgctaaat tatcctcagg cgaagttatt actcccagag
     5881 aaatacgtgg tcagatgtct ttgggaatgc tttgttcagc taaagaactt ggtttaggag
     5941 aagatgattc aggtattatg attctagata aatcaaaagt gaagcagtta ggtcaaactt
     6001 tggatgatta tcttactaat taatttagtt aatatatgga aatattttca acttctcatg
     6061 atttagttaa tctagcttta acaatcggca ttattggtgt tagcgcggct atagtttatt
     6121 tctttattgt tgcttctaat tttattcgag agttgcatcg aaccattgat gaacttaatc
     6181 gtcagcttga gacaattagc cagataaccg aatctattaa aggtaaattg tctttcatgg
     6241 tgtcttattg ggcgatttta gaaaaggtgg gtagcaaagt aatagattta ttccaaaata
     6301 aaatgtttag cagaataaaa gataaagttt ttcattcttc aactaaaaaa cataaaaaaa
     6361 gtgaagaaga aaatgattta gacaataatc aaatcgaagt agaaccaaaa tctgaaacta
     6421 aggttaaaac taaggctaaa attaagaaat ctaaaaaaaa taagacgaag gttagctaaa
     6481 atttgttgag ttgggtttag tagttttttg tatcttttta atgttgcgac acttagggaa
     6541 agcgctacaa gcgagaaaag gtccatatcg ccctattttt ataaccattt tggcgccaca
     6601 tttatcacaa gtttcattag catattcttt agctaaatat tgatactttt cgggtaatgg
     6661 tttgggagta gatggtgatt ttagtgattt aatgttgcga cacttaggga aagcgctaca
     6721 agcgagaaag ggtccatatc gccctatttt tatgaccatt ttggcgccac atttatcaca
     6781 agtttcattg gtaggtgtct cgggcataat atctttttta gataattctt ggtttttttc
     6841 ttctaaatgt tgatgaaatg gccaataaaa atcggcaata acctttggcc acgccacctc
     6901 tcctctagca atatcatcta aattattttc tatattggca gtaaattgat agtcaacaat
     6961 ctcaggaaag tgttttacta ataaatcgtt aactatcttg ccgatagctg ttggtgctag
     7021 tcgtttataa ttatctcttg ccacatagcc tcgcttttca atggtcgcaa taattggagc
     7081 ataagtagat ggccggccaa taccgtattt ttctaattgt tttaccagcc cagcgtcgct
     7141 ataacgagcc ggtggttgag taaagtgttg atctgttgtt agctctagta atttaagatg
     7201 ctctttgtca gtaaggtgtg gcaaattttt ttcttgtgtt tgctcagggt agatacttaa
     7261 atatccagga aaggtaattg tctgaccagt agctagaaat atagccgagt tagccttaat
     7321 gtctatagag agatggttaa tttgggcttc tgctgcttgg ctagctaaag ctcgctgcca
     7381 aataagctca taaagtctaa attgacgtgg atctaagtat tgtttaattg attctggtgt
     7441 ccgttggggg ttagtcggtc gaatagcttc atgagcttcc tgggcccttt tatttttatt
     7501 aataaacttg cggggagtct tagctataaa ttgatggcca aaattatctt ggataacctt
     7561 tcgggcctga tggataaact cactggataa atctagagcg tcggttctca tgtaagtaat
     7621 gaggccaact tgtccttcag aacctaaatt aataccttcg tatagttgtt gagctagaac
     7681 catggtttgt ctagctgaaa agcctagcca gcggttagct acttgctgta aagtagaagt
     7741 agtaaaggga gggggtggca tttttagaat tttttcttta gtgattttat caataacaaa
     7801 ttcttctccg gtgagttttt ctttaatttc ttcggctttt tgttggtcag caagagcaaa
     7861 tttctccaat gtttttcctt gccattttac taattgagca gtaaattttt cttcatgagc
     7921 ttgtaaatga gcggtgattg tccaatattc ttgtttaatg aatttttcta tttctctttc
     7981 ccgctcgaca attaatctta cggccactga ttgcacccgc ccggctgata aattttttgc
     8041 tacctttttc cagagaaaag gagacagttc atagccaacc aatctatcca gaattcttcg
     8101 ggcttgttga gcgttaatta aattataatc aagttctcga ggcgctttga gagcattttt
     8161 aatagctgat tcagttattt catgaaatac aatgcgattt ttttgattag ccggtagttt
     8221 taatagttgc tgaaggtgcc aggcaattgc ttcaccttct cggtcttcgt cagtagctag
     8281 aatgatttca tcagcttttt tggctagagt ttttaattta gctactacct ttttagcctt
     8341 agccggcacc tcatattctg gctcaaagtt gttttctata tctacaccca attttttagt
     8401 aggtaaatca cgtacatggc caaatgatga ctcaacagtg tcagtttttg ttaaaaaatg
     8461 gctaatggtt ttagctttag tgggcgattc cacaataatt aaacgcatat ttagttggtg
     8521 attgtttgtt ttaatttatt ccactatacg gagttgctag gggcaagtca agtattactc
     8581 tggaattttt tcagatagtg tggactggga gtaatttgcc ggtagtgttt aagtttagcc
     8641 atccccctat aaccattaat actaataata ataaagtcat agcgccattt atttatttta
     8701 tatctctcca aaaaataatt agctgctatt tgtagttgtt tttcttggta gctccccatt
     8761 attttattgg tatagctaaa tttattagta agatatagtt ttatttctat aaagacaatg
     8821 atagatcgtt gttgggctac taaatcaatt ttttgttgat gataatgcca attactggcg
     8881 ataattttat agccagtttt ttgaaaataa gccaatgcaa ccgcttggcc aaaattggtc
     8941 caggggttgt tttgtttagc catgatttat agtggagggt ttttagaagg taggtttatc
     9001 tggacttaaa ttaaaataat tcacaagtta tccactgctt taaattttag ctaatattag
     9061 ccacgaagta aataatttgt ctagattttt atggactttt ccaccgaata atttgtctag
     9121 atttttatgg acagaataaa tgatttttaa caatattatt tggttccaca tggaacaaaa
     9181 acgaggattg tggaccataa gggacttgaa cccttggcct cttccatgcc atggaagcgc
     9241 tctagccaac tgagctaatg gcccatttat taatctccgt gcccccacca tgaatcgaac
     9301 atggatttag ggcttaggag tcccttgttc tatccattga actatgaggg cctaattttg
     9361 tccaacagag gtaagtgctt ggtattgtaa gggaaaaata attgttcgtc aaggatattt
     9421 taaaaacaaa aaggaagcca ctgcgggccc ttcatttaca taaagatgta aaaaattgaa
     9481 atggccccga cggcctcctc cttgaaattt ttataaaaat ttcaagagca aaattcttaa
     9541 accggcttca aggaactttt ggtctccttt atggtgacct tggccctttt attttttgtt
     9601 tttttatttt ttaggagaag ccagagtaag aaggtaaaaa agaactatag agatagtata
     9661 gcataaaata aaaaagtggt caagacttct gactacattc ctctagactt aatgttttat
     9721 tgactaaaat aattttttag atttcaaatt ataaaattat tttagtctat attcttttag
     9781 acttaacaaa gaaagataat ccctggaggt cacggtggga gtcgaaccca cgtataacag
     9841 ttttgcaaac tgttgcctta acctcttggc gacgtgaccg attaaattag tctaacaaaa
     9901 aagatttggt cgggcaagta taattttttt agtgtatgtt tagccaataa ttatagggtc
     9961 taattgactg agacacccaa aaatgataga gtgaggctga taattagaat aaacatattg
    10021 gtaagtccaa tgtcagccga tcaatgaagg ctttctttgt tgacggttca ttatttttat
    10081 taaatatttt acttaaagtc aaaaggatta ttgtttggct attgtcgcgt atttttaaat
    10141 tagtcggtcg gttatttatc tattggccag cgactaagat ctacaagcta tatttgcact
    10201 tgcgccggcg gatgattaac cgctatcaac aaaatgcttg gctggcagta attacgttcc
    10261 tattaatagc tctgtttatt gttttataca accagcaaga aagcaatcag ttgatggctg
    10321 ccgaagattt agtccgtggc acatggttat ccagattagt tggtgatgag tttaatcaat
    10381 ttaatgatat tgtggtggat aaccagccac cccgagaatt tattgttaat aacccagcta
    10441 ttattactaa taatctaagt accttagatc tggctaaatc tactgacctg acgactgttt
    10501 tgggaatgac aactgaccgc tgggcgtata ctttgggtcc agttagtctg cctcaagtaa
    10561 atatccaagg cactacctct actgctcctt ctagggctat tacttattat accgttaaaa
    10621 ttggcgatac tttaagcggt attgctaaag cttttggggt aagcataaat acaattgctt
    10681 ggcaaaatgg tttgagccct aacgcccttc tacggcctgg tgataaattg accattttgc
    10741 cagttagcgg agttttacat aaagtgaagg ccggagatag tttatctaag attgctaaac
    10801 aatatggagt taagactgct gatattattg cctataacaa tttatctggc gataaagtag
    10861 ttattggcca gtctttaatt attcctggcg gccagccacc atcaacactg gctagtcaaa
    10921 tcactcaagc cgttaagagt aatgtccaac aagtttttac ttcagctaaa gagacggctc
    10981 aaaagagttt aacctcatca ggtagcggta tgacctggcc aacggtaggc catcgtatta
    11041 ctcaatatta ttcgtggcgt catactggta ttgatattgg taataaagtg gggacaccga
    11101 tttatgccgc cgatagcggt cgggtagaaa tagccgctac tggttggaat ggtggctatg
    11161 gcactactat tgttattaac catggtaaat taaagactcg ctatggccat ttgtcacgtc
    11221 tatatgttaa agcgggagaa aatgtggtaa agggtcaggt tattggtgag atgggttcga
    11281 ctggccgttc tactgggtca catttgcatt ttgaggtttt atctggtaat gttaggtata
    11341 atccgttaaa ttatgtccat taaattatct ctatatgtta cattttttgg gtggattagc
    11401 catcgtggtt ttgggggcag tcattatctt aaaacgagaa tggattttaa ataattttgg
    11461 ccgtatagat ttttttgaag agaagctgag cgcctatggc ggaaccagaa tggcttattc
    11521 acttattggg cttattgcta tttttattgg cttttgtatt gcctttgggt taataaataa
    11581 ttttttaggt tggatgcttg gtccattact gcggtatcaa cagagttaaa tatattttgg
    11641 gcctttagct cagctggcta gagcgtctgc tttgcaagca ggaggtcgag ggttcgagtc
    11701 ccttaaggtc caccacattg aactaattgc ctcactcggg gctttgcttg atgttttaat
    11761 gaaataaaaa tatggcacta t
//
LOCUS       DAOOIS010000013        11751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_5015_length_11751_cov_38.442288, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000013 DAOOIS010000000
VERSION     DAOOIS010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11751
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5015_length_11751_cov_38.442288"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="PK960_02010"
     CDS             <1..1253
                     /locus_tag="PK960_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPD07972.1"
                     /translation="LRLLQGDVGSGKTAVAILAMANVADSGYQTALIAPSEVLAYQHF
                     QTVVASLGEFFVIGLLTRTQQFLSKPKEGIKKTTKKQLVRLLSLGQCQVVIGTQALLQ
                     EPVDFYKLGLLIVDEQHRFGVEQRQLLLNKKLFAENGQNLVAHFLAMTATPIPRSLAL
                     SLYGDLNLSVIRTMPAGRLPVITKIVEDKDRAKMYKFIRQLLDKGEQAFIVCPLVDPS
                     DKLAVKAATDEFNYLVNGELKGYKVGLLHGRLSGANKQKVMADFAAGRYQVLVATAVV
                     ELGLDIPQATVMVIEGADRFGLAQLHQYRGRVGRRGQQAYCFLLPSPASGELAKRRLK
                     AVVKYNNGQELARLDLKWRGPGELYGYEQTGFSELRLANIFDQDLISAAHEIALEVLH
                     HPNWRYRAITNLAWQLGGLDVLPQ"
     gene            complement(1261..2616)
                     /gene="radA"
                     /locus_tag="PK960_02015"
     CDS             complement(1261..2616)
                     /gene="radA"
                     /locus_tag="PK960_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPD07973.1"
                     /translation="MPTIFTCTKCGAQAPKWSGRCNECGAWGTMVEEAMPKNPLGPKQ
                     HLSSSKPAQLINWHELPSKPIHRYVTGLIEIDRVLGGGLVPGSLILLSGEPGCGKSTL
                     VAQIAQTLDPQLSIIYISGEESAQQVKMRLDRLNYQPQKMTFASETNAETIIATAIKY
                     KPQLLIIDSIQTVYSDEVPSEPGSVNQIKACTIKLLELAKEYNITVILIGHLTKEGIV
                     AGPKTLEHLVDTVIYLENESQHSYSLIRASKNRFGSTNEVGLLEMTGHGFRQLEKTTS
                     LFIQPIQGASGSAISCLIEGSRPFLLEVQALVSKTYFGYPQRKAIGFDSNRLQILTTV
                     LTKQTKINLTDQDVIVSVAGGLKINETALDLAVCAAIASSYYNRPLPDKTLFLGEVGL
                     AGEIRPIGKLEIRLKEALKIGISQVFLPAQAKITSSKMKIKTSNNLLDFFNQVWPKTD
                     K"
     gene            complement(2635..3435)
                     /locus_tag="PK960_02020"
     CDS             complement(2635..3435)
                     /locus_tag="PK960_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPD07974.1"
                     /translation="MTKTTKKKNINLHQNIDNPWIISIVLACVGICLVFLFSVLINWP
                     KWIVSQSSVGPTTIYTLKDNFDEQINNEGNLTTKTTISPHLLGGPIISPDDPSRGAEQ
                     ALINIVYFGDFACNFCQEQEQIFEQALNTYPDKIRIIWKDLPENNTSSLSYQAAKAGR
                     CAFLQNHFWEYHDALLSSNSLQPDNLETTAIKVGLDLNSFRQCLQTPNNLAETLINKN
                     WQEADGLGISATPYTYVNNRSFMGKLSWEELKTVIDYELAQLGSGQSE"
     gene            complement(3445..4710)
                     /locus_tag="PK960_02025"
     CDS             complement(3445..4710)
                     /locus_tag="PK960_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07975.1"
                     /translation="MNFFSKYKRLIFIILFIIVVIGIAYLLYIVFFRQNINPSLVSEN
                     PPEENAEIQLPEAGPNLNIKPTGSSEENVPSAEKNEVYGPITGTEESVDNKAMGGVTK
                     TDKVIGGITINPTINNQTGDIQYYDPFDGKFYTVSEDGTIRPLSNKVFYNVQQVTWSK
                     NSDKAVLEYPDGSNVVYDFNKQQQVTLPKHWQEFDFSPNSNQLVMKSLGMDVDNYFLA
                     VANSDGTGSRVIEFIGENYKKVYPSWSPNNQVVALYAEGLDYDRQTVYFIGLNNENFK
                     SMTIPGRGFQFQWSPQGDRLLYSVYSSDNEMKPMLWIADASGSQIGENRQRLNLQTWA
                     NKCTFANGEEIYCAVPTSLDYGAGLMPALADQTPDYIYKINTKNGARQLVAIPDSNIT
                     VSSIMISPDKSKLFLVDKNTGSIRQIKLH"
     gene            4832..5914
                     /gene="pilM"
                     /locus_tag="PK960_02030"
     CDS             4832..5914
                     /gene="pilM"
                     /locus_tag="PK960_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPD07976.1"
                     /translation="MALFKSEANDSCLGVDIGTSGIKIVELKRDDKRVVLSTYGFSES
                     LGGNNPLNMPIEDAAKTITKIWQDAGMTSRRVVASLPNFSVFSSIITLVDVSDANLSM
                     AVNAEAKKVMPLPLEEMVLDWKKIPVPIGQQVGPTNSNAADIKSGAKSITRILLTGAP
                     KVLVNKYIEIFKAAELNLVFLEGEIIALIRSLVGNDKGIIMIVEVGSTNTDLVIIDHG
                     VPVFNRSLDLGGLTVTETISKNLNIDLQQAEQFKYDLGVAGIRSAELPEALKSLVDSV
                     ASEIKYSLNLFQSNNGKSVEKIIMTGGSSLLYNFPEYLSKALNMNVIIGSPWTLISYP
                     LNIKPLLDEIGPRLAVAVGLAMRGIS"
     gene            5925..7328
                     /locus_tag="PK960_02035"
     CDS             5925..7328
                     /locus_tag="PK960_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07977.1"
                     /translation="MLGSKSSKKVKQPDQSFTTKEKVDWSSTEGYEAMKEVNNFLKWP
                     SVWAGKKSQPAREVDFSAKQSKRRGAKQLMSNGGTFNSQKSTIKQSSTDIIEPVQTPS
                     AQVESTPLSSGGKQLMVGPVQEPQKEGNLWKSLKSKLAARKLKKINEERSRQIKQSAD
                     SVSNRSQQVKGSIFHTKEEQRRQVAKLSSNKLTEVSDLKQAKPSVIQPINKANIEKSS
                     GDVHLRKEKEISETVSKAPIQTLNKKDEQQRFREMQQSVEQKSWTRPAIMETNLVGGQ
                     TTLYFNWRRFWLDLTQWLIVTLFFLSLCGALFYLWRQNQIRQTADVDDRLAKMNQLTN
                     LAENEVSDVLDFRLRLILVNELLDNHIYWNNFFSFLEKNTLANVFYQDFSGDTSGEYI
                     LKARTDNFDSMAKQIKVFRQSPDVLEVDTEGGEIIKNEPGTTEAGEGSEGEVGSSTRS
                     QLNFSIKLKINPDLFFR"
     gene            7331..8020
                     /locus_tag="PK960_02040"
     CDS             7331..8020
                     /locus_tag="PK960_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07978.1"
                     /translation="MAQLLNKKITLRKSTTKSNSRTLAGQTTFQQRWLEFIINYYYIF
                     IALICIFIMVGSVLFFFLPQYRLTLSELNANNQRAQIELVEKQKYQAKLVQLNDFYKS
                     IDQNTRNKVMDILPVQLDKENLLAEIEAICLANNLLIKSSDVVVEPSSADNNTSESAS
                     SSTSTNTDLSGHLRSAIINLELSGRDYINFKKLLKTIENNLHLMDIEKLEYLPGKNQI
                     ILNIKIYYLAD"
     gene            8027..8449
                     /locus_tag="PK960_02045"
     CDS             8027..8449
                     /locus_tag="PK960_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07979.1"
                     /translation="MEKIIRSKKRNQLFTSKTLWLILGIIINTVGLFLVVRWYGQFTS
                     NQRPFVNVDESVFKVFANSISEKQPLSPEQRRLLKTSLTQQSVEEFLSLSLLNNPKFQ
                     NLRLNYIPPEVTPTSSTTTVPEEDLTGLKIGNPHPFGY"
     gene            8460..10211
                     /locus_tag="PK960_02050"
     CDS             8460..10211
                     /locus_tag="PK960_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPD07980.1"
                     /translation="MSKTFDNKLLDFLVSANLINYEQLTEVNEALAAKGANLDQILID
                     KGILDWERLTEVKASLAGLSYVNLADMKIDEAALNILPPEVAGNYQVVCFQREGDEIS
                     VGLVDPENFKAIEAVDFLAKKSNLKVHYFLVSPLSFSLTFKKYQTINKEISSALELKA
                     KEEEREVRESLEKNDLRFEEITKNAPISKIISAIIRHGVELGASDIHIEPLPKESRIR
                     YRVDGILKTHLILPKNIHDSLVARVKVMSKLKLDETRIPQDGRISLNIDGRDIDFRVS
                     VMPLMGHEKVVMRILDVSRGAPTLESLGFASNQYRVIKKIIERTSGLILITGPTGSGK
                     TTTLYSLLNILNQDNVNIATLEDPVEYFLKGVNQSQVRPEVNFTFATGLRSLLRQDPD
                     IMMVGEIRDNETAELAVHASLTGHLVLSTLHTNDAIGAIPRLIDMKVEPFLLASSLIL
                     VVAQRLVRVNCSHCLTEEKVSGDVLNYIKETLAKVPPEVMKERLPNYNPDQMTFNKGR
                     GCAYCDKTGYHSRTVIAEILEINDQVRDMILQNESFSENNVKTTQSFITMEQDGFIKA
                     LQGLTTVEEVMRVIQSD"
     gene            10216..11415
                     /locus_tag="PK960_02055"
     CDS             10216..11415
                     /locus_tag="PK960_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPD07981.1"
                     /translation="MIFRYEALDNQGAKTTGLVEADDSQEANSLLQDNGLTLVSLEVA
                     HIRKGLSFAFLKRVSPKDVVIFARQFSIMIGAGLPMVQALRILVNQTENVNFKKVIGE
                     IGDEVEGGASLSDSLAKRPKIFSNFFVSVVKSGERSGKLDEVLDYLADEIGRDYDMMS
                     KIKGSMIYPTVVIVGMVIIGVVMMVFVLPKLMDILVETGTTLPLSTRILMGISNFITH
                     YWWVVALVITGLVLGIKAILNTTSGRYAWDSLTLHLPVFGKLFQKILLVRFCRSMNTL
                     LLGGVSIENSLAIAGDVVTNKVYQKLVQQTIKEVEDGNSISTIFLESPLVPKMVSHMM
                     QVGENTGKLDVVFTKIAEFYGREIDNTLANLMSLMEPLIMIILGVGVGVMVAAVILPM
                     YTMVGNF"
BASE COUNT         3569 a         1845 c         2428 g         3909 t
ORIGIN      
        1 tgctgcgctt gcttcagggt gatgtaggga gcgggaagac agcagtagct attttagcaa
       61 tggctaatgt agcagatagt ggttaccaga cagcattaat tgcaccaagt gaagtattgg
      121 cttatcagca ttttcagaca gttgtggcga gtttggggga attttttgtc attggtctac
      181 ttactagaac tcaacaattt ttaagtaaac ccaaggaggg tattaaaaag actacaaaaa
      241 agcaattggt cagactgtta tccctgggac aatgccaagt tgttattggt actcaggctt
      301 tgcttcagga gcccgttgat ttctataaat tgggcctgct catagttgat gagcaacatc
      361 gttttggggt agagcagcgg cagttgttgc ttaataagaa attatttgcg gaaaatggtc
      421 aaaacttagt ggctcatttt ttggccatga cagctactcc tattccgcgc agtttagctt
      481 taagtcttta tggtgatttg aatttgtctg tgataagaac aatgcctgct ggtcgattac
      541 cggttattac taaaattgtt gaagataagg atagagccaa gatgtacaaa tttattcgtc
      601 agcttttaga taaaggagag caggctttta ttgtttgtcc tttagtagac ccttcagata
      661 aattagcagt taaagcggcg accgatgaat ttaattattt agtgaacggt gaattgaaag
      721 gttataaagt ggggctgctt catggccgtt tgtctggggc taataagcaa aaggttatgg
      781 ctgattttgc cgcgggacgc taccaggttt tagtggctac agcagtggta gagttgggac
      841 tagatattcc tcaggctacg gttatggtta ttgaaggggc tgatcgtttt ggtttggctc
      901 aactgcatca atatcgggga cgagtaggcc gacgggggca gcaagcttat tgttttttat
      961 taccatcacc ggctagcggg gaattggcga aacggcgact gaaagcagta gtaaaatata
     1021 ataatggcca agaactagcc cggctggatt taaaatggcg gggacctggt gaactttatg
     1081 gctatgaaca aacaggtttt tctgaattgc ggttagctaa tatttttgat caggatttaa
     1141 tttcagcagc tcatgaaatc gctttagagg ttttacatca tcctaactgg cgttatcggg
     1201 ctattactaa tttagcttgg caattagggg gattagatgt cttaccccaa tagtgactta
     1261 ttatttatct gtctttggcc aaacttgatt aaagaaatct agcaaattat tactagtttt
     1321 tattttcatt ttgctactgg tgattttggc ttgagctggt aaaaacactt ggcttatacc
     1381 tatttttaga gcttctttaa ggcgtatttc taatttgcca attggtctaa tttccccggc
     1441 tagtcctact tctcccaaga aaagagtttt atctgggagt ggtcggttat aataactaga
     1501 ggcaatagct gcacagacag ctaaatctag ggcggtttca ttgattttta gaccaccggc
     1561 cacactgaca ataacatctt ggtctgttag attgattttg gtttgcttag ttaagacagt
     1621 agttaaaatt tggaggcggt ttgagtcaaa gccgatagcc ttgcgttgcg gatagccaaa
     1681 ataagttttg ctaactaggg cctgtacttc taataaaaat ggacggctgc cttctatgag
     1741 acagctaatg gctgagccac tagctccttg gatgggctgg ataaataggc tagtagtttt
     1801 ttctagctgg cggaaaccat gcccagtcat ttctaagaga ccgacttcat tagtggaacc
     1861 aaagcgattt ttactagctc taatgagact atagctatgc tgtgattcat tttctaagta
     1921 aataacagta tcgactaaat gttctagggt ttttggtccg gccactattc cttctttagt
     1981 taagtgtcca attaaaatga ctgtaatatt atattcctta gctaactcta agagtttgat
     2041 agtacaagct ttaatttgat taacactccc gggctcagat ggcacttcgt cggagtaaac
     2101 tgtttgaata gaatcaataa ttaacaattg aggtttatat ttaatagcag tggcaataat
     2161 agtttcagca ttggtttcac tagcaaaagt catcttttgc ggttgataat ttaatctgtc
     2221 tagccgcatt ttaacttgct gagctgattc ttcgccgcta atataaatta tggacaattg
     2281 aggatctaat gtttgggcta tttgggctac taaagttgac ttgccacagc ctggctcacc
     2341 acttaataaa ataagggagc cagggactag accgccacct aacacccggt caatttcaat
     2401 aaggccagta acatagcggt gaatgggttt actgggtagt tcgtgccaat taattagttg
     2461 agctggtttg ctactagata agtgttgctt agggccaagt ggatttttag gcatagcttc
     2521 ttctaccatt gtaccccagg cgccacattc attgcagcgg cctgaccatt ttggggcttg
     2581 agctccacat ttagtgcaag taaatatagt tggcatggtg atattctaga atatttattc
     2641 tgattggcca ctacctagtt gggctaattc ataatcaatg actgtcttta gttcttccca
     2701 gctaagtttt cccataaaac ttcggttatt aacataggta tatggagtag cactaatacc
     2761 taaaccatca gcttcttgcc agtttttatt aattaaagtt tcagctagat tatttggagt
     2821 ttgcaaacat tgacgaaaag aatttaaatc caggccgact ttaatggctg ttgtttctaa
     2881 gttatctggt tgtagggagt tactactaag taaggcgtca tgatactccc aaaaatgatt
     2941 ttgtaaaaaa gcacaacggc cagcctttgc agcttgatat gataaactgg aagtattatt
     3001 ttctggtaga tctttccaaa ttattctaat tttatctggg taggtgttta gggcttgctc
     3061 aaaaatttgt tcttgctcct gacaaaaatt acaggcaaaa tcaccgaaat agactatatt
     3121 aattagcgct tgttcggcac cacgactagg gtcatcagga gaaataattg gtccacctaa
     3181 gagatgagga cttatagtcg ttttggtggt taaattgcct tcattattaa tttgttcgtc
     3241 aaagttgtct tttagggtat aaattgtcgt gggcccaaca gaggattgac tgactatcca
     3301 cttaggccaa ttaattaaga cactgaacag aaacactaaa caaataccaa cacaagccaa
     3361 aactatacta ataatccaag gattatctat attttgatgc aaatttatat tttttttctt
     3421 agttgttttt gtcatagtaa tttattaatg aagtttaatt tgacggattg agccggtatt
     3481 tttatccact agaaagagct tagatttatc tggacttatc attatactgc tgactgttat
     3541 attagaatca ggaatcgcaa ctaattgtct ggcaccattt ttggtattga ttttatatat
     3601 ataatctggt gtttggtcag ctagagcggg cataaggcca gccccataat ctaggctagt
     3661 ggggacagca caatatattt cttcaccatt ggcgaaagtg catttattag cccaagtttg
     3721 taggttaagt ctttgtcgat tctccccaat ttggctacca gaagcatcgg ctatccagag
     3781 cattggtttc atttcattgt cactagaata tacactatag agtagtctat caccctgagg
     3841 cgaccattga aattgaaaac cacgtcctgg tattgtcata gacttaaaat tttcattatt
     3901 aagtcctata aaatatacag tttgtcgatc atagtctaga ccttccgcat atagggcgac
     3961 tacttgatta ttgggtgacc agctaggata aactttttta tagttttctc cgatgaattc
     4021 aattactcta cttcccgtgc catcactatt agctacagct aaaaagtaat tatccacatc
     4081 cattccaaga cttttcatga ctaattggtt actgttagga gagaaatcaa actcttgcca
     4141 atgttttggc agggtcactt gctgctgttt attaaaatca tagacgacat tactgccatc
     4201 gggatattct agaacagcct tatcactatt tttagaccat gttacctgtt gaacattata
     4261 aaaaacctta ttagatagag gacgaatagt accatcctca cttactgtat aaaatttacc
     4321 gtcaaaaggg tcataatatt gaatatcacc ggtttggtta ttgatagttg gattaatggt
     4381 tatgccccca ataactttat cagttttggt tacaccaccc atggctttat tatcaactga
     4441 ttcttcagta cctgtaatgg ggccataaac ctcatttttt tctgctgatg gcacattttc
     4501 ttcgctagaa ccagttggtt ttatatttaa gtttggtcca gcctcgggta gttgaatttc
     4561 tgcgttttct tcaggtggat tttcggaaac tagactggga ttaatatttt ggcgaaagaa
     4621 aactatatac agtaaataag ctataccaat aacaacaatg ataaagagga ttatgaaaat
     4681 taaccgttta tattttgaaa aaaaattcat attaatatgc tattgtggct aattagttaa
     4741 ttatgctata atgttaacaa tctgttatta ttatagaaga ttgtaagatt atcgtcaaaa
     4801 tttattttat tttaatttct gtttttatat tatggcttta tttaaatctg aagcgaatga
     4861 tagctgttta ggtgtagata ttgggacttc aggtattaaa atagttgagc ttaaacgtga
     4921 tgacaaacga gtggttttgt ctacttatgg ttttagtgag agtttaggtg gtaataatcc
     4981 tcttaatatg ccaattgaag atgcagctaa gacgattact aaaatttggc aagatgccgg
     5041 gatgaccagt cggcgcgtgg ttgctagttt acctaatttt tcggtttttt cttcaattat
     5101 tactttagtt gatgtcagtg atgctaattt gtcaatggcc gttaatgctg aggctaaaaa
     5161 agtaatgcct ttgccactag aggaaatggt tttagactgg aaaaaaatcc ctgtacccat
     5221 tggacagcaa gttggaccca ctaatagtaa tgcagccgac attaaaagtg gcgctaaatc
     5281 tataactaga attttattaa ctggcgcacc taaggttctg gtaaataaat atatagagat
     5341 ttttaaagca gctgaattaa atttggtgtt tttagagggt gaaattattg ctttaattcg
     5401 ttctcttgtt ggtaatgata agggaattat tatgatagtt gaggtaggct ctactaatac
     5461 tgatttggtt attattgacc atggtgtgcc ggtatttaat cgcagcctag atttaggggg
     5521 gttaactgtt acggaaacca ttagtaagaa tttaaatatt gatttacaac aggccgaaca
     5581 atttaaatat gatttaggag tagcaggtat taggtcagct gagttgccag aagccctgaa
     5641 aagtctagtt gattcagtag ccagtgaaat aaaatattct ttaaatttat ttcaaagcaa
     5701 taatggtaaa agtgtagaaa aaataattat gactggtggt tcttctttac tttataactt
     5761 tcctgaatat ttatctaaag cattaaatat gaatgtcatt attggtagtc cctggacatt
     5821 aatttcttat cctttaaata ttaaaccatt acttgatgaa attggtccaa ggttagcagt
     5881 agctgttggt ttagctatgc gtggaattag ctaattataa acaaatgtta ggcagtaaat
     5941 cttctaaaaa agttaaacaa cccgatcaat catttaccac taaagagaag gtagattggt
     6001 cttctactga aggttatgag gcaatgaaag aagttaataa ttttttaaag tggccgtcag
     6061 tctgggctgg caaaaaatca caacctgctc gtgaggtgga ttttagtgct aaacaatcta
     6121 aacgtcgggg ggctaagcaa ttaatgtcaa atgggggtac ttttaactcc caaaagtcaa
     6181 ccataaagca atcctcaacc gatataatag agccagttca aacaccatca gctcaagttg
     6241 agtctactcc cttatcttcg ggtggtaagc agttgatggt gggtcctgtg caagaacccc
     6301 agaaggaagg taatttatgg aagtctttaa agagtaaact cgcagctcgt aagctaaaaa
     6361 aaattaatga agagcgaagt cgccagatta aacaatcagc cgattcggtt tctaatagga
     6421 gtcaacaagt taaaggttca atttttcata ctaaagaaga acaacgtcgt caggttgcta
     6481 agcttagtag taataaattg actgaagtct cagacttaaa acaggcaaaa ccgtcagtta
     6541 tacagccaat caataaagct aatattgaaa aatcttcagg agatgttcac cttagaaaag
     6601 aaaaggaaat ttcagaaact gtctctaaag cacccattca aaccctcaat aaaaaagatg
     6661 aacaacaaag atttcgcgag atgcaacaga gtgtggaaca aaaatcatgg actcggccag
     6721 caattatgga aactaattta gttggggggc agaccacttt atattttaat tggcgtaggt
     6781 tctggctaga cttaacgcaa tggctaatag tgactttgtt ttttctatct ttgtgtggtg
     6841 ctttgtttta tctgtggcgt caaaatcaaa ttaggcaaac tgctgatgtt gatgatcgtc
     6901 tagctaaaat gaaccagcta accaatctag ctgaaaatga agttagcgat gttttggatt
     6961 ttcgtttgag gctaatttta gtcaatgaat tattagataa tcacatttat tggaataatt
     7021 tttttagttt tctagagaag aatactttag ccaatgtttt ttatcaagat ttcagtggtg
     7081 atactagcgg ggagtatatt ctgaaagccc ggactgataa ttttgatagt atggctaagc
     7141 agataaaggt tttccgtcag tcgccggatg ttttagaagt ggatactgaa ggtggtgaaa
     7201 ttatcaaaaa tgagcctggc actactgaag caggagaagg cagcgaagga gaagttggga
     7261 gctctactcg tagtcaatta aatttttcaa ttaagctaaa aattaatcct gatttatttt
     7321 tccgttgagt atggcccaat tattaaataa aaaaattact ttacgaaaat caaccacgaa
     7381 gtctaattcc agaaccttgg caggacagac tacctttcaa caacgctggc tagagtttat
     7441 tattaattat tattatattt ttatagccct tatttgtata tttattatgg ttggtagcgt
     7501 cttattcttt tttttaccac aatatcgttt aacattgtca gagcttaatg ccaataatca
     7561 acgagcccaa atagagttag tggagaaaca aaaatatcaa gctaaacttg tacagttaaa
     7621 tgatttttat aaaagcattg accagaatac ccggaataag gtaatggata tattgccagt
     7681 acaattagac aaagaaaatc tgttggccga gattgaggct atctgtttag ctaataattt
     7741 gttaattaag tcttctgatg tagtagtaga accatcatcg gcagacaata atactagtga
     7801 atcggcgtct tcttctactt ctactaatac tgatttaagt ggtcatctta ggtcagcaat
     7861 tattaatttg gagctatcag gacgtgatta tattaatttt aagaaattgc tcaagactat
     7921 tgagaacaac ttacatctta tggatattga gaaattagaa tatctaccgg gcaaaaatca
     7981 gattatttta aatattaaaa tttattattt ggctgattag taaattatgg aaaaaattat
     8041 tcgctctaaa aaaagaaatc agctatttac ctccaagaca ttatggctta ttttgggaat
     8101 tattattaat actgttggtt tatttttggt ggtgcggtgg tatggtcaat ttactagtaa
     8161 tcagcggcca tttgttaatg tagatgagtc agtatttaag gtttttgcca attctatatc
     8221 tgagaaacaa ccattgtcgc cagaacagcg tcgactatta aaaactagtt taactcagca
     8281 gtcagtggaa gaatttttat cgttgagcct attaaataat cctaaatttc aaaatttacg
     8341 gcttaattat ataccgccag aagttactcc taccagtagt actaccactg tgcctgaaga
     8401 agatttaact ggtcttaaaa ttggtaatcc tcatcctttt ggctattgac ttatattgta
     8461 tgagcaaaac ttttgataac aaattattgg attttttggt ctcagctaat cttattaact
     8521 atgaacaatt aactgaggtt aatgaagctc tagcggctaa aggagctaat ttagaccaaa
     8581 ttttaataga taaggggatc ttagactggg aacgtttaac tgaggttaaa gctagtttag
     8641 ctggcttatc atatgttaat ttagcggaca tgaaaattga tgaagctgct ttaaatatat
     8701 taccaccaga agtggcaggg aattatcagg tggtttgttt tcaacgcgag ggggacgaaa
     8761 ttagtgtagg tcttgtagat cctgagaatt ttaaagctat cgaagcagtt gattttttag
     8821 ctaagaaaag taatctgaag gttcattatt ttttagtatc acccttaagt tttagtctta
     8881 ccttcaaaaa atatcaaaca attaacaaag agatatctag tgcattggag ttaaaggcga
     8941 aagaagaaga gcgggaggtt agagagtcgt tggaaaaaaa tgatcttcgc tttgaggaaa
     9001 ttactaaaaa tgctcctatc tctaaaatta tttcagccat tataaggcat ggagttgaac
     9061 tgggcgccag tgatattcat attgagcctt tgcccaagga aagccgcatt cgttatcggg
     9121 tagatggtat tcttaaaact catttaattt tgcctaaaaa tatccatgat tcattggttg
     9181 ctcgggtgaa ggttatgtcc aaattaaaac tagatgaaac tcgtattcct caagatggtc
     9241 gtattagttt aaatattgat ggccgagata ttgattttcg tgtgtcagta atgccactca
     9301 tggggcatga gaaagtagtc atgaggattt tggatgttag tcgtggggcg cctacccttg
     9361 aatcccttgg ttttgctagt aatcaatatc gggtaattaa aaaaattatt gaacggacaa
     9421 gcggtcttat attaattact ggaccaactg gttctggtaa aacgaccact ctttattctt
     9481 tacttaatat tcttaatcaa gataatgtta atattgccac cctagaagat ccagtggaat
     9541 attttttgaa aggtgttaat caatctcagg tccgaccgga ggttaatttt acttttgcta
     9601 ctggcttacg ttctttactt cgtcaagatc cagatattat gatggttggt gaaattcggg
     9661 ataatgagac cgctgaatta gcagttcatg cttcattgac tggccattta gtactttcca
     9721 ctttgcacac caatgatgcc attggggcca ttccacggct tattgatatg aaggttgagc
     9781 cgtttttatt ggcctctagt ttgattttgg ttgtagcaca acggttggtg cgagttaatt
     9841 gttctcactg tttgactgaa gaaaaagtaa gcggtgatgt attaaattat attaaggaga
     9901 cactagctaa agtaccacca gaagtaatga aagagagatt acctaattat aatcctgacc
     9961 agatgacttt taataaagga aggggttgtg cttattgtga taaaactggt tatcattctc
    10021 gtacagttat tgctgagatt ttagaaatta atgatcaagt gagagatatg attttacaaa
    10081 atgaaagttt tagcgaaaat aatgttaaaa ctacccaatc atttattacc atggagcaag
    10141 atggtttcat aaaggctttg caaggattaa caaccgtaga agaggttatg agggttattc
    10201 agtcagatta aatatatgat tttccgctat gaggcgctag acaatcaagg cgctaagaca
    10261 acggggttgg ttgaggctga tgatagccaa gaggccaatt ctttgttgca agataatggt
    10321 ttaaccttgg tgagtttaga ggtagctcat attagaaaag gattgagctt tgcttttttg
    10381 aaacgtgtat ctcctaagga tgttgttatt tttgctcggc aattttctat catgattggt
    10441 gctggactac caatggtgca agctctgcgc attttagtta atcagactga aaatgttaat
    10501 tttaagaaag ttattgggga aattggtgat gaagtggagg gtggagcatc tctatcagat
    10561 agtttagcta aaagacctaa aatattctct aatttttttg ttagtgtagt aaaatctggc
    10621 gagcgttccg gtaaactaga tgaggtgttg gattatttag ctgatgaaat tggcagggat
    10681 tatgatatga tgtctaaaat taaaggctca atgatttatc ctacagtagt aattgttggt
    10741 atggtaatta ttggtgtggt aatgatggtt tttgttttac ctaagttaat ggatatttta
    10801 gtagagactg gcacaacttt accattatct acaagaatat taatgggtat ttctaatttt
    10861 ataacccatt attggtgggt agtagcacta gtaataacgg gattggttct ggggataaaa
    10921 gctattttaa atactacatc tggtcgttat gcctgggact ctttaaccct tcatttgccg
    10981 gtatttggta aattatttca aaaaattttg ctagttcgtt tttgccgttc aatgaatact
    11041 ttattactag gtggggtaag tattgaaaat agtttagcta ttgctggtga tgtagtaacc
    11101 aacaaggttt accaaaaatt agtgcagcag actattaaag aagtagaaga cggtaattct
    11161 atttctacta tctttttaga gagtccactg gtacctaaaa tggtatctca catgatgcag
    11221 gtaggtgaaa atactggtaa gctggatgta gtttttacca aaattgctga gttctatgga
    11281 cgggaaatag ataataccct agccaatcta atgagtttaa tggaaccgct tatcatgatt
    11341 attcttggtg ttggcgttgg agtaatggta gctgctgtta ttttgccaat gtataccatg
    11401 gttggtaatt tttaagttag ctaaaatcgg ggaactttgt ttagatagcg gttgagtttt
    11461 tgttgttaat agggggatag actagcgaca gttactgggg gtctaggcaa taattttttt
    11521 ttattgtata ataatataca gcaaattttc ttttaataat tttgtctatt aaattaattt
    11581 tatgaaaaaa ggttttacct tagttgagct cttagtagtt attgccatca ttggcattct
    11641 ttctactttg tcagtggtgt ctttaaatag cgctagagcc aaatcccgtg atgctcgtcg
    11701 cctttcggat attaagcaaa tcaggacagc gctagatatg tattatgatt c
//
LOCUS       DAOOIS010000014        10364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_5780_length_10364_cov_37.022602, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000014 DAOOIS010000000
VERSION     DAOOIS010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10364
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5780_length_10364_cov_37.022602"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /gene="miaA"
                     /locus_tag="PK960_02060"
     CDS             complement(<1..923)
                     /gene="miaA"
                     /locus_tag="PK960_02060"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPD07982.1"
                     /translation="MVNNNHKAVAVIGPTAAGKTKLAVALASKYHGEIISADSRQVYR
                     GMNIGTGKDLDDYYNNGHPIPYHLIDIVEPTENFDVATYQRLARQALNNILARGHLPI
                     VAGGSGLYLQALIDNYEFSPAKPDTALRQDLEKLSAQELYQKLNNLAPALASKLNNSD
                     RHNPRRLIRYLEIILNKPTISPPRYHDQTNWLILGIDYPMEILEQRIYDRLIERLENE
                     DMIEEVYHLHEEGVDWHRLESFGLEYRFISRYLREQLDYDQMVEQLYIAIRQFAKRQK
                     SWFKRWEKQGREIHWLNINNPLQEAESLIDN"
     gene            complement(931..2205)
                     /gene="miaB"
                     /locus_tag="PK960_02065"
     CDS             complement(931..2205)
                     /gene="miaB"
                     /locus_tag="PK960_02065"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPD07983.1"
                     /translation="MAKTYHIITIGCAMNQSDSERLASRLNELGWCRVSDCHQADLVV
                     LVTCGVRQSAEDRIYGLINQIKKANPKTAIAITGCLVYRQDIKTKLQDKVIYWFSINE
                     LGRLEQILPCYNPDNIKPIKDYLEIMPDYKSRVSAFVPIGNGCNNFCSYCVVPYARGR
                     EIYRPAQDIIVEVQQLLNKGYQEITIIAQNVNSYKDELNGLDFTGLLEKLDSLGDYWL
                     RFATSHPKDMSDRLINFLGQSHNLIHHLHLPVQSGSNKILKAMNRNYTAEYYRTLINK
                     VRQQWPDIILTTDIIVGFPGETDKDFADTIQLVKDLKFDQVYVARYSPRPGTLAAKMA
                     DDVPAQVKKKREQQLNQMVIAGALKNRQAQIGQRVMVLLEEQRGDCYFGHTDHGLQIL
                     VTTPEKKSYTLGLRYLAQITSARSFSLAGQLL"
     gene            complement(2210..3307)
                     /gene="dnaJ"
                     /locus_tag="PK960_02070"
     CDS             complement(2210..3307)
                     /gene="dnaJ"
                     /locus_tag="PK960_02070"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPD07984.1"
                     /translation="MAKDYYQILGVPKTASTEDIKKAFHKLAHQYHPDKASGNEAKFK
                     EINEAYQVLSDKNKRAQYDRFGSADFQGSQAGPDSRGGFDFSQFYRGEGNNGFNINFG
                     DFGDFGDIFGDLFGFKNTRTNHKTSNQGRDLEISIKIDFLTSIFGGEQTITLDKLVPC
                     PSCQGSGSQPGASYSTCPNCGGRGFINQVQRTMFGSMNTQTVCPTCEGEGTIISQPCS
                     KCHGEGRILEKQSLTIKIPAGVSNGQTIKFTGQGEAGRRGGPSGDLYVKVKVQPHPRL
                     KREGDDIKSTVNISFRQAALGDKIKIETVDGPVVLKIPPGTQSGQVIILRGKGVPKLH
                     GYGRGNHLIEVRVITPTKLTKEQREALEKLD"
     gene            complement(3379..5277)
                     /gene="dnaK"
                     /locus_tag="PK960_02075"
     CDS             complement(3379..5277)
                     /gene="dnaK"
                     /locus_tag="PK960_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPD07985.1"
                     /translation="MSKIIGIDLGTTNSAVAVMEAGHPKILENAEGGRTTPSVVAISK
                     NGERLVGQAAKRQAIINPENTIYAIKRLIGRRYQDSEVQRDIKSLPYTIEQAGDGIKV
                     KMQDKYYTPQEISAMILSKLKADAEARLGEKVTEAVITVPAYFDDSQRQATKDAGKIA
                     GLEVKRIINEPTAAALAYGFDKKKGQQIAVYDLGGGTFDISILDISEDTVEVKATNGN
                     THLGGEDFDQRIINWIIDEFKKDQGIDLSKDTLALQRIKEAAEKAKIELSTLTETEIN
                     QPFITTDANGPKHLVMKITRAQLENLVGDLVEQTIEPCKKALADAGYKVGDIEEVILV
                     GGMTRMPMVQAKVKEFFGKEPNLSVNPDEVVALGAAVQAGVLQGDVKDVLLLDVTPLT
                     LGIETLGGVATPLIERNTTIPTSKSQIFSTAADGQTSVEIHVVQGERPMAADNKSLGR
                     FILDGIPPAPRGIPQIEVTFDIDANGILNVKAKDKASGKEQKITITASSGLSKEEIER
                     LRQEAEQHAAEDQKKKEAAELKNQADSVIFQLEKLLKESGDKMKAEDKTQLEAKLADL
                     KKAKEGEDLENLRQQMTEINQLAQTIGASMYQQSAGTNSDTNSSSNNPGGENPNNGPV
                     EGEIEENQ"
     gene            complement(5292..5732)
                     /locus_tag="PK960_02080"
     CDS             complement(5292..5732)
                     /locus_tag="PK960_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPD07986.1"
                     /translation="MRDLVRWNPLFDSLDEAEKIFNDFLPALRSDQSGFVPAIDMYED
                     KDNIVVETQLAGIQPENVQISIDNDTLCIKGESEHKKEVDEKNYYRKEIRRGSFYRSV
                     QLPAHVQGDKAKAVAEDGVLKVIIPKAPEAKPKTIKIEAKKANK"
     gene            complement(5795..6343)
                     /locus_tag="PK960_02085"
     CDS             complement(5795..6343)
                     /locus_tag="PK960_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPD07987.1"
                     /translation="MSTTTSKKKDNKEAKNEQNESVKTVKLGEYQELESKYKRALADQ
                     QNLVKQHQKDRSHYIKYANEQLLLTILPIYDNLKLAYKHFNLDNPSSWLDGLKYIIKQ
                     FKDTLEQEGVTEIETHNQLFDHHSMEAVEIIKTNNKEQDNIVAEEVRSGYKLADRIII
                     PARVKVYHYEDHQNGSMPLDHN"
     gene            complement(6312..6998)
                     /locus_tag="PK960_02090"
     CDS             complement(6312..6998)
                     /locus_tag="PK960_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HPD07988.1"
                     /translation="MEQRPALILKLLTEEYIETATPVASQTLVEKYNLDFSPATVRND
                     LAELEERGYIKQPHTSAGRIPTELAYRYLINQLNKGTPSRKTQTMLKPYFNDLNSNWK
                     QLAKTLAQLTGNGIFWSFHRYDSYYTGLSGLLSQPEFKQLPLVYDISQVIDSLEEIIN
                     DLFDKIPEKPIIRLGSDSPFGPFSSTILTKYRYGEHLGLFGLLAPLRNNYQENFNLIN
                     YVYDHIQEKR"
     gene            complement(7112..7699)
                     /locus_tag="PK960_02095"
     CDS             complement(7112..7699)
                     /locus_tag="PK960_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07989.1"
                     /translation="MLEKLFSSQARMKLLKLFLFAQPEQAFYVRQLSRQLNLQLNSVR
                     RELANLEDIGLLKVKSDNHKADKKFYQVNQDFYLLEELRTLFAKAEVGQENQLVHDLQ
                     KLGSIDLLILTGFFTNDALAPTDILIVSNHFNKNKLNDLINNLEQASGHAIRYTLMTK
                     EEFNYRESMADIFLYKILHSHKKIIINKLLSEEGI"
     gene            7804..10197
                     /locus_tag="PK960_02100"
     CDS             7804..10197
                     /locus_tag="PK960_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07990.1"
                     /translation="MPYKLASLLLSAEQKSSSIAKIAWAEADHGDESNELRFARVGKI
                     FSLIEINATSAKAKLLLDFFSERPKLNYYGQPEIDQNEYPSPEDLEKLFTTAIQETNH
                     DLINFLEEHKIRSVQPEISATIGLLTEKKIFLASIGHNQVWLLLPRWPSGQQVIKIIG
                     TPKDNEIGNNYFFQEIISGVLPNHSYCLISNEALAEYIEPATALNLLANKPPLIGAEK
                     LKNFLQQTNRRVDFLGLIIKRVPFSELQDDEEQLAAVKINQPTTSRTQLVSSNLQLTE
                     KQTEKILNPFGWSKLGSILKKTSVSLWQWLVRLIHRPWLDDCRRYWQKACHQLATIKH
                     KIKTWPIWLLISSSGHKFLVFIKSLWYKTQPKLQSLGHHISAWWFKSAPWRCRLVEKW
                     WWPIERHLRPIKNRLFKLRQRVAHCFRYRCGSRHSWRNLKRRSRIFLILFSLCCLFLI
                     GGTIWQHQRQISQTRLQEQMDELALVEQKHKQIEANLLYNNLTAANQSLDEAEELIKK
                     WEETQPKVPEDINKRWQEAILEQEELIGKVRHIYTLHNGEIIAGQPNIVVTSSDKLLG
                     LSGQLMAWVNNSEKKIVYQNDQGEWQITSWPEGWEEISAMAADNNNWYFATASGQLAS
                     LNHSGQWKLWSLPEPVKQIKALALFSGRLYLIDENNQIRRYDIKEDTLVNGQKWNTDP
                     LPVGEIAGLAINGQVYVFYRDGQAFKLNNGRKQNFTITASEPLLTGWSSCQLSSDGSS
                     FDCLESGGSRIIIYKLDGTILSQYLLADISAQSMTINHKDKIIYTLTNEGIKKFIMP"
     gene            10265..>10364
                     /locus_tag="PK960_02105"
     CDS             10265..>10364
                     /locus_tag="PK960_02105"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPD07991.1"
                     /translation="MIVQNHKSHYSAFTLVELLVVIAIIGILSTLSV"
BASE COUNT         3043 a         1978 c         1868 g         3475 t
ORIGIN      
        1 aagttatcta ttaaagattc tgcttcttgc agtggattat ttatattaag ccagtgaatt
       61 tctctcccct gtttttccca gcgtttaaac caactttttt gacgtttggc aaattgacgg
      121 atagcgatat atagctgttc taccatttga tcataatcta attgttctct aagataacgg
      181 ctaataaaac gatattctag gccaaaggat tctagtcgat gccaatctac cccctcctcg
      241 tgtaggtggt aaacctcttc aatcatgtct tcattttcta aacgttcaat taggcggtca
      301 taaattcgct gttctaatat ttccattgga taatctatgc ctagtattag ccagtttgtt
      361 tggtcatgat aacgaggtgg cgagattgtt ggtttattta gaataatttc taaataacga
      421 attaatcgcc gtggattatg gcggtcgcta ttatttaatt tactggctaa ggctggagct
      481 aaattgttta acttttgata aagctcctga gctgataatt tttctaagtc ttggcgtagg
      541 gcggtatcag gtttcgctgg actaaattca tagttatcaa taagggcttg taaataaaga
      601 ccactaccac cagccacaat tggcaaatgt cctcgagcta aaatattgtt taaagcttgg
      661 cgggctaacc gttgataagt cgctacatca aaattttctg tcggctcaac aatatcgatt
      721 aaatgataag ggataggatg accattatta taataatcat ctaagtcttt gccggtgcca
      781 atattcattc ccctatatac ttgacggcta tcggcactaa taatttctcc gtgatattta
      841 ctggccaagg caacagctaa tttggttttg ccagcggctg ttggcccaat gactgctact
      901 gctttatgat tgttattaac catagattta ttataaaagc tgaccagcta gactaaaact
      961 tctagcgcta gttatttggg caagataacg aaggcctaaa gtatagcttt tcttttctgg
     1021 tgtagttacc aaaatttgta gtccatggtc agtatgacca aaataacagt cccccctttg
     1081 ttcttctaac aaaaccatta ctctttgacc aatttgcgct tgacggttct tgagggctcc
     1141 agctattacc atttgattta attgttgttc tcgctttttt ttaacttgag ccggtacatc
     1201 atcggccatc ttagccgcta acgtcccggg acgcggacta tatctagcaa catagacttg
     1261 atcaaatttt aaatctttta ctaattggat agtgtctgca aaatctttat cagtttctcc
     1321 gggaaagccg acaataatat cggtagttaa aataatgtct ggccattgtt ggcgtacttt
     1381 attaattaat gtccgataat actcagcagt gtaattacgg ttcatcgctt taagtatttt
     1441 attgcttcct gattgaactg gtaaatggag atgatgaatt aaattgtggc tttggcctaa
     1501 aaaattaatc aaacggtcgc tcatatcttt ggggtggctt gtagcaaaac gtagccaata
     1561 atcacccaaa ctatctagct tttctaaaag accagtaaaa tccagtccat ttagttcatc
     1621 tttatagcta ttaacatttt gggcaatgat tgttatctct tggtagcctt tatttaacaa
     1681 ttgttgaact tcaacaataa tatcttgagc tggccggtat atttctcggc ctcgggcgta
     1741 aggcactaca cagtagctac agaaattatt gcagccatta ccaataggca cgaaagcgct
     1801 aacccttgac ttataatctg gcattatttc taggtagtct ttaattggtt ttatgttatc
     1861 tgggttataa caaggtaaaa tttgttccag tcgccctaat tcattaatgc taaaccaata
     1921 aattacctta tcttgtaatt tagttttaat atcttggcgg taaactaggc aaccggttat
     1981 agctatggca gttttggggt tggctttttt aatttgatta attaatccgt aaatacgatc
     2041 ttcagcgctt tggcggacac cacaagttac taaaactact aaatcagcct gatggcagtc
     2101 gctgactctg caccagccta attcattaag acggctagct aaacgctcgc tgtcactttg
     2161 gttcatggca cagccaatgg taataatatg gtatgttttg gccattgatt tagtctaatt
     2221 tttctaaagc ttccctttgc tctttagtaa gtttagtggg agtgataact cttacttcaa
     2281 ttaaatgatt gcctcggcca tagccatgta gttttggtac tcccttgcct cgcagaataa
     2341 taacttggcc actttgtgtg ccaggcggta tttttagtac aactggaccg tctactgttt
     2401 caatttttat tttatcccct aaagcagctt ggcggaaaga aatattaact gttgatttga
     2461 tatcatcacc ctcccgctta agacgggggt gtggttggac tttaacttta acataaaggt
     2521 caccacttgg tccacctctc cgaccagcct caccttggcc agtaaattta atagtttgtc
     2581 cattagatac cccggcgggt attttaatag ttaaagattg tttttctaaa attcgaccct
     2641 cgccatgaca tttagaacag ggctgagaga taatagttcc ttctccttcg caagtaggac
     2701 atactgtttg ggtattcatg ctaccaaaca tagtcctttg gacttgatta ataaatcccc
     2761 ggccaccaca attagggcag gtagaataag aagcgccagg ttgactaccg ctgccctggc
     2821 atgagggaca gggcactaat ttgtctagag taatggtctg ctcaccacca aaaatagatg
     2881 ttagaaaatc gattttaata ctaatttcta aatcgcgacc ctgattacta gttttatgat
     2941 tagttcgggt atttttaaaa ccaaataaat cgccaaatat atcaccaaaa tctccaaaat
     3001 cgccgaaatt aatattaaat ccattattcc cctcaccacg ataaaattga ctaaaatcaa
     3061 aaccgcccct actatctggt cctgcttggc tgccttgaaa atcagcactg ccaaaacgat
     3121 catattgagc tcgtttattt ttatcactta aaacctggta agcttcattt atttccttaa
     3181 acttagcctc gttaccacta gctttatcag gatgatactg atgggctaat ttatgaaaag
     3241 cctttttgat atcttcggta gaagcggttt taggtactcc taaaatttgg taataatctt
     3301 tagccataaa ttatctgctt attaagcagc ctgaggccat attggcttcc ggctgcctca
     3361 aagtaggtaa tagtaagttt attgattctc ttcaatttca ccctccactg gcccattatt
     3421 tgggttctcg cctccaggat tattagaact agaattagta tcagaattag taccagctga
     3481 ttgctgatac attgaagcgc caatagtttg cgccagttga ttaatttccg tcatttgttg
     3541 gcgtaaattc tctaaatctt caccttcttt agctttcttt aaatcagcca atttagcttc
     3601 cagttgggtt ttatcttcgg ccttcatttt atctcctgac tcttttaaga gtttttccaa
     3661 ctgaaaaata acactatcgg cttgattttt aagctcagcc gcttctttct tcttctgatc
     3721 ctcagcggca tgctgttctg cttcttgacg caatctttca atttcttctt tacttaagcc
     3781 agatgaagca gtaatggtaa ttttttgttc tttacccgag gccttatctt tagctttaac
     3841 atttaagata ccattagcgt caatatcaaa agtaacttca atttgtggaa taccacgtgg
     3901 agctggagga ataccatcta agataaaacg tcctagggat ttattgtcag cggccatggg
     3961 tcgttcaccc tgaacaacat gaatttctac acttgtctgt ccgtcagcgg cggtagagaa
     4021 aatttgactt ttggaggtag gaatagtggt attacgctca attaatggcg tagctactcc
     4081 ccctagcgtt tcaatgccta aagtaagggg cgtaacatca agtagaagca cgtcttttac
     4141 atcaccctga agtacgccag cctgtacggc tgcccccaga gctactactt catcaggatt
     4201 tacacttaaa ttaggctctt tgccaaagaa ctctttcact ttagcctgta ccatgggcat
     4261 acgggtcata ccccccacta gaataacttc ttcaatatcg cccactttat aaccggcatc
     4321 agctaaagct ttcttacaag gctcaatggt ttgttccact aaatccccta ctaaattttc
     4381 taattgagcc ctggttattt tcattactaa atgtttaggt ccattagcat cagtagtaat
     4441 aaacggctgg ttaatttctg tctcggttaa ggtggataat tcaattttag ctttttcagc
     4501 tgcttcttta atccgttgca gggctaaagt atctttagat agatcgattc cctgatcttt
     4561 tttaaactca tcaataatcc aattaataat ccgctggtca aaatcttcac cccctaaatg
     4621 agtattgcca ttagtagcct ttacttctac agtatcttcc gaaatatcta gaattgagat
     4681 atcaaaagta ccgccaccca aatcatatac tgcgatttgt tgtccctttt tcttgtcaaa
     4741 accataggct aaagctgcgg ccgttggctc attaataatt cgtttaactt ccaggccagc
     4801 aattttgccg gcatctttag tagcttgacg ttgggaatca tcaaaataag ccgggacagt
     4861 aattaccgcc tctgttactt tttcgcctaa acgagcttca gcatccgctt ttaacttaga
     4921 aagaatcatg gccgaaattt cttgcggcgt ataatattta tcctgcattt tcaccttaat
     4981 accgtcacca gcctgttcta tggtataggg caagctttta atatcccgtt gaacttcact
     5041 atcttgatag cgtcgaccga ttagtcgttt aatggcatag atggtattct caggattaat
     5101 aatagcttgt cgtttagcag cttgaccgac taaacgttca ccatttttag aaatggctac
     5161 taccgaaggt gtggtccggc caccctcggc attttctagg attttgggat ggccggcctc
     5221 cataaccgct actgcactat tggttgtccc taaatctatt ccaataattt tactcataat
     5281 tgtgtagtaa attatttatt ggctttttta gcttcaattt tgatagtctt tggtttggct
     5341 tcaggagctt taggaataat aacttttaat actccatctt cggcaactgc tttggcctta
     5401 tcgccttgta cgtgggctgg taattgcacg ctgcgataga aactaccacg acgaatttct
     5461 ttccggtaat aattcttttc atcaacttcc tttttgtgtt cactttcacc cttaatgcat
     5521 aaggtatcat tgtcaataga gatttgaaca ttctctggct ggataccagc taattgtgtc
     5581 tcgacaacga tattgtcttt gtcttcatac atatcaattg ccggaacaaa accactttga
     5641 tcacttctga gagcgggcaa aaaatcatta aaaatttttt ctgcctcatc tagactgtca
     5701 aaaagtggat tccatcttac taagtctcgc atattgttgg ttgaccgctt ctattttctt
     5761 ttgggggaca aataaaagct ggtcgctaaa ttaattaatt atgatcaagc ggcattgagc
     5821 cgttttgatg atcttcataa tgataaactt tcactctggc tggaataata attcgatcag
     5881 ctaacttata accagacctg acttcctcag ctactatatt atcttgttct ttattattgg
     5941 ttttaataat ttctacggct tccatactat gatggtcaaa tagctgattg tgggtttcaa
     6001 tttcagttac accttcttgt tctaaagtat ctttaaattg tttaataatg tactttaggc
     6061 catctaacca actagaagga ttatctaaat taaaatgttt ataggctaat ttaagattgt
     6121 cgtaaattgg taatattgtc agcagtaact gttcattagc atatttaata taatgactgc
     6181 ggtctttctg atgttgcttt actagatttt gttggtcggc taaggcccgt ttatatttac
     6241 tttctaattc ttggtactca ccaagtttaa ctgtttttac tgattcattt tgttcatttt
     6301 tggcttcttt attatctttt ttcttggatg tggtcgtaga cataatttat taagttaaaa
     6361 ttttcttgat aattatttct taagggcgct aagaggccaa ataaaccaag atgctcgccg
     6421 taacggtatt tagtcaaaat agtgctagag aaagggccaa atgggctatc gctacctaaa
     6481 cggataattg gtttttctgg tattttgtca aataagtcat taataatttc ttctaagcta
     6541 tctataacct gcgaaatatc atacactaat ggtaattgtt taaattccgg ctgacttaaa
     6601 agaccgctta agccagtata ataagaatca tagcggtgga atgaccagaa gataccatta
     6661 ccggttaact gagctaaggt tttagctaat tgtttccaat tactatttaa atcattaaag
     6721 tacggcttaa gcattgtttg cgtcttacga ctaggtgttc ctttattaag ttgattaatc
     6781 agatagcgat aagctaattc agtgggaata cggccagctg aggtatgagg ttgtttaata
     6841 tatcctctct cttctaactc ggctaaatca ttgcgcaccg tcgctgggga gaaatccagg
     6901 ttatattttt ctactaaggt ctgagaagcc actggcgtgg ctgtctcaat gtactcttca
     6961 gtcaataatt ttaagattaa agccggccgt tgttccatat gtcttatgtt aagcaattat
     7021 aagtatagca tccttagcag tcaagtgtca agagtgctaa actaaaaaca acctaacata
     7081 aattaggctg tttttattag gttttgagca attatatccc ctcctcggat agtagtttat
     7141 taattataat ttttttatga ctatgaagaa ttttatacaa aaaaatgtca gccatgcttt
     7201 cgcgataatt aaattcttct ttagtcatca aagtatagcg aatagcatgg cccgaggctt
     7261 gttccaaatt attaataagg tcatttaatt tatttttatt aaagtgatta ctgacaatta
     7321 atatatcagt aggcgctaag gcatcattgg taaaaaagcc ggttagaatt agaaggtcaa
     7381 ttgaacctaa tttttgtaag tcatgaacta attgattttc ttggcctact tcggctttag
     7441 caaataatgt ccttaattcc tctaaaagat aaaaatcttg gttaacttga taaaattttt
     7501 tatcagcctt atgattgtcg cttttaacct ttagaaggcc aatgtcctct agattagcta
     7561 attcccggcg gactgaattt aattgaagat taagttgtcg tgataattga cgaacataaa
     7621 aagcctgttc tggctgagca aacaaaaaaa gcttaagtaa cttcattctg gcctgtgagc
     7681 taaataattt ttctagcata ttaagagctg tataaacttt ttatcagtcc ttgtctaaaa
     7741 caataatatc ttatataatt actttcgtca aggttgttta gcccaatttt ttagtatata
     7801 tttatgccat ataaacttgc ctctctcctc ttaagtgccg agcaaaaaag cagctcaatt
     7861 gctaaaattg cctgggctga agctgatcat ggcgacgagt ctaatgagtt acgttttgct
     7921 cgagtaggca aaatttttag cttaatagaa attaatgcta ctagtgctaa ggctaaatta
     7981 ttactagatt tttttagtga acgaccaaaa cttaattatt atggacaacc agagatagat
     8041 caaaatgaat atcccagccc agaagattta gaaaaattat tcactaccgc catccaagaa
     8101 actaatcatg acttaattaa ttttttagaa gaacataaaa ttcgttcagt ccaaccagaa
     8161 ataagtgcga ctattggtct tttaactgaa aaaaaaatat tcctagcttc tattggtcat
     8221 aatcaagttt ggcttctatt acctagatgg cccagcggcc agcaagttat taaaatcatt
     8281 ggtactccaa aagacaacga gataggtaat aattattttt ttcaagaaat tattagtggg
     8341 gtactcccta atcacagtta ttgtctaatt agtaatgaag ccttagctga atatatagaa
     8401 ccggctactg ctcttaattt attagctaat aagcccccac ttattggggc tgaaaaatta
     8461 aaaaattttt tacagcaaac taaccggcgg gtagattttt taggcttaat tattaaacga
     8521 gttcctttta gtgaactgca ggatgatgag gagcaattag cagctgttaa aattaatcag
     8581 ccgacaacta gtaggactca attagttagt agtaatttac aacttactga aaaacaaaca
     8641 gaaaaaattc ttaatccttt tggctggtca aaattaggta gtatattaaa aaagaccagt
     8701 gtgagcctat ggcaatggtt agtaaggtta attcatcgcc cctggctaga tgattgtcgc
     8761 cgttattggc agaaagcttg ccatcaatta gcaactataa aacataaaat taagacttgg
     8821 cctatctggt tgttaataag ctcaagtggt cataaatttt tagtctttat taaaagttta
     8881 tggtataaaa ctcagcccaa gttacaatct ctgggccatc atatttccgc ctggtggttt
     8941 aagtctgccc catggagatg tcggctagtt gaaaaatggt ggtggccaat tgagcgtcat
     9001 ttaaggccaa taaagaacag attatttaaa ttacgtcaaa gggtggctca ctgctttcgt
     9061 tatcgttgtg gttcgcgcca ttcatggcgt aatcttaaaa gacggagtcg cattttttta
     9121 attctattta gtctgtgttg tctttttctt attggtggta ctatttggca gcatcaacga
     9181 caaataagtc aaactaggct gcaggaacaa atggatgagc tagctttagt ggagcagaaa
     9241 cataaacaaa ttgaggctaa tttattatat aataatttaa ccgcggccaa tcaaagctta
     9301 gatgaagctg aagaattaat taaaaaatgg gaggaaactc aacctaaggt gcccgaggat
     9361 attaataaac gttggcaaga agctatttta gagcaagaag aacttatagg aaaagtacgc
     9421 catatttata ctctccataa tggagaaatc attgctggcc aacccaatat tgttgtaact
     9481 tctagcgata aattattggg acttagcggt caattaatgg cctgggtgaa taatagtgaa
     9541 aagaaaattg tttatcaaaa tgatcaagga gagtggcaaa ttacttcttg gccagagggc
     9601 tgggaagaaa tcagtgctat ggctgccgac aataataact ggtattttgc taccgccagt
     9661 ggccagctag cttcacttaa tcatagtggt cagtggaaac tatggtcgct ccctgagcca
     9721 gtcaagcaaa ttaaggcttt agcccttttt agtggtcgtc tttatcttat tgatgaaaat
     9781 aatcaaataa gacgttatga tataaaagaa gacacattgg tgaatggtca aaaatggaat
     9841 accgatcccc tgcccgttgg tgagattgcc ggactagcca ttaatggaca agtttatgtc
     9901 ttttaccgtg acggacaagc ttttaaatta aataacggcc gtaaacaaaa ttttactata
     9961 accgccagcg aaccattact tacgggttgg tctagttgtc aactatcttc tgatggctct
    10021 agctttgact gcctagaatc tggtggctca agaattatta tctataaact agacgggaca
    10081 atattaagtc aatatctttt agctgacatt tcagcccagt ccatgactat taatcacaag
    10141 gataaaatta tttatacctt aactaatgaa ggcataaaga aatttataat gccatagtta
    10201 tcaatttata atctggaaac taaatcctag aaaaatcaag tagatgatga taaaataaat
    10261 aaacatgata gttcaaaatc ataaatccca ttattcagct tttactttgg ttgaacttct
    10321 cgtagttatt gccattattg gtattctttc tactttatcc gtag
//
LOCUS       DAOOIS010000015        10114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_5929_length_10114_cov_42.401531, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000015 DAOOIS010000000
VERSION     DAOOIS010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10114
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5929_length_10114_cov_42.401531"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PK960_02110"
     CDS             complement(<1..202)
                     /locus_tag="PK960_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07992.1"
                     /translation="MSPQELSLTRDAEALHTLSIGCRTLDEASAILDNPHCAEKTKNL
                     ILAKIQLFDKAELARVNKNPEQY"
     gene            354..1205
                     /locus_tag="PK960_02115"
     CDS             354..1205
                     /locus_tag="PK960_02115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPD07993.1"
                     /translation="MRLLDSHAHVNFNAFKDDGDEVIRKSLEAGVGMILVGSEFRTSN
                     RAIDYANHYETGVWAAIGLHPSQLFATRVQGEDYDFMSRGEELDLEKYDKLADFSKVV
                     AIGEIGLDYYRLADINESPKVIKEKQQRVLSQELTLAMHHHLPVIIHCREAHDDLLPL
                     LQDFYHDYRQILPKDKPWGVVHCFSGDMGLAQEYFSLGLDISFNGLITFCSTWDYLIQ
                     KMPLDRLLIETDCPFLTPVPFRGQRNEPSLVKLVAERIAQIRHTTIECIAEATTANAC
                     RLFNIKW"
     gene            1211..2275
                     /locus_tag="PK960_02120"
     CDS             1211..2275
                     /locus_tag="PK960_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPD07994.1"
                     /translation="MTIEIILGVGLVLAVAALIWLITRQETGSEKIVALNEKLETLTN
                     QNNELRALVDEKLRETYQASQNQYQNTMNTVQSINQLLTDVTKHLAELQASNKQILGF
                     SGQLQNLQDILRNPKQRGVLGEYFLEEILKNVLPPGTFQMQYQFANGEIVDAVIFVKD
                     KIIPIDSKFSLENYEKIINTSDPGERLRLEKLFRQDLKNRIDETSKYVRPEVGTMDFA
                     FMFIPSEAIYYDLLVNKVGAVQIDTRDLIEYAFRDKHVIIVSPTSFLAYLQTVLQGLK
                     ALQIEENAKIIKSNVEKLSRHLSSYEDFLRRLGGSLGTAVNHYNQAYQEFKKVDRDVV
                     KITESDHRVEPMVLERPHEE"
     gene            2278..4608
                     /locus_tag="PK960_02125"
     CDS             2278..4608
                     /locus_tag="PK960_02125"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPD07995.1"
                     /translation="MTSRFVERRQQNAWTLESAIRETVAYFSLFQYPLTLWEIWSFLP
                     EKADLLAVKREVDRLVKEEKLYERWGYYFLPGEAGAVDCRAERYPITIAKIKRAKKVA
                     KWLRWLPGIQFIGLANVMGSYNLRSDSDIDFFIITKPGSLWFCRFWANLMMQLVGLRP
                     TDAKRSNKICLSFWLASNDLRLDRFKLPDEDGYPDWYYIYWLANLRPLVEQGINYQQI
                     IKSNPWLKTYLPNWRPSIISQSGGNFWQRVASFFSRFGIVRAKIWESTLAYWQQKFLP
                     ANARTLMNIDERVVVSKEVIKLHIIDRREYYRSKLRQKLEAIARGEKGTIRPDEPVRR
                     LPPNWWLRLGNIAATILVLILPWSTRWILHPGELGGEFWEYGTISIYLSDIVLLLSCL
                     AVKLGEVSSPRISTKDSSKKYYLGWLSGLWLISAVSLLVNGVSWSLVIFYLLRLIIWS
                     GGWYFLLGHLKWPWPRWATLFLASLIVPALLGLGQFIGQDASGNKWLGLASHHPGDLG
                     VAVIESTNAAGEVTGRWLRAYGSFDHPNILGALMAIAIILIMVASQYYESNLSRFLLF
                     ASVGLWSLTALVSWSRTAWLAILFGWLISLMLIGRKDRHRRQFLVVFLLGLLLWSGLW
                     FWPFKDLGRSRVSGEPRLEQKSLVERKESYGEALRIIREHLWLGVGLGNYTGFLSRQN
                     ETIGPAWNYQPVHNSFLLIISEIGLLGFVWLAVGLVMLLRSASKRLEIIWPLGVSFIW
                     LLLMDHWWWSLHSGIVYFWLVISSLYWISRQPRNLL"
     gene            4661..>5420
                     /locus_tag="PK960_02130"
     CDS             4661..>5420
                     /locus_tag="PK960_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD07996.1"
                     /translation="MKKIYLSSFLIVALGLVGWQLGHHLLPTAGAAPSPSCTIMITNI
                     DPKQYSPDRVDTILYDSGLITKGAFVVASKNGALPFQTQAAAQTTIDSAISQIIKEVC
                     GGNCNPNALTMDIHCPSSSCPTSVLSFTVFDPKQYNPDTVDTILYDFGLITKGAFVVA
                     SKNGALPFQTQAAAQTTIDSAISQIIKEVCGGKCNPQALQIVPQCVTESPVNVARQAY
                     EQRLSNMNDCQKQVFQAMQANNEQYLAKVKKNNQQ"
     assembly_gap    5421..5520
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5575..6444
                     /locus_tag="PK960_02135"
     CDS             5575..6444
                     /locus_tag="PK960_02135"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPD07997.1"
                     /translation="MLIFKHILSVMNLDKPLVIFDLETTGLALAYDRIIELAFVKIMP
                     NGRVIEKDIFFNPEIDVPSEVTAINGLTNADLADKPLFRDHAQEIWDIFYDCYYGGFN
                     VVNFDLPMLRREFLRAGYDFNYTKKQVIDSKVIYHAMEPRDLAAAYKYYCQKELANAH
                     SALADVRASAEILDQQLAKYDYGLIRQISEAPDDRYVDEEKRFYWRSGEAYLNFSKYK
                     DRSLAEIAKVDPGFLRWMMEADFSPETKQIVERALEGEFPKKQSQDSTSSNDIVAPAK
                     KSMSGFVEEPKLF"
     gene            6446..7480
                     /gene="ruvB"
                     /locus_tag="PK960_02140"
     CDS             6446..7480
                     /gene="ruvB"
                     /locus_tag="PK960_02140"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPD07998.1"
                     /translation="MSKNLKKISKDGLLAADLASDSDEALDLSLRPQTLSEYVGQPAI
                     HQSLEIFLQAAQSRGENIEHVLLYGAPGLGKTTLAHVIANELKANLRVTSGPAIEKAG
                     DLAAILTNLQDGDLLFIDEIHRLPRSVEEILYPAMEDGALDIVLGKGPSARILRLDLP
                     RFSLIGATTKISLLSAPLRDRFGMVYRLDFYGVEDLEKIIKRSAKILQIELEPSASHL
                     IAERARRTPRIANRLLKRVRDYSQVKGSGAITEDICRQAFELLAIDDLGLDNMDRLLL
                     ETIIDKFGGGPVGLNTLAASTGEELITIEDIYEPYLLQLGFLERTPRGRKVTVKAYKH
                     LNRVPPDSLF"
     gene            7482..8543
                     /gene="queA"
                     /locus_tag="PK960_02145"
     CDS             7482..8543
                     /gene="queA"
                     /locus_tag="PK960_02145"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPD07999.1"
                     /translation="MDLSDFDYFLPPELIAQAPAEPRDRAKLMVVNRQTGTWQTTIFS
                     NLPQFLQPSDILIVNNSKVLPARLIGHKAGTGGKIEVLLEHKLVSPSGQESWRCLIGG
                     NRPKVGLIINFPQDLTGQITAEEGDGVFTVEFNKSGSDFWSAVYAIGEMPLPPYIKAD
                     KTTIKEAVKDYQTVYAKDNKVGSVAAPTAGLHFTKELLKRIKAMGVEIVEVTLHVGLG
                     TFEPVRDENIKEQRLHEELVEIGAQKINQLIKYHQAGRRLIAVGTTSVRVLEGVAAHF
                     IDTLATDDWLAPIDLFISPGFEFKLLDGLITNFHLPKSSLIMLVSAWAGTDLIKKTYA
                     AAIAEHYRFFSYGDAMFLV"
     gene            8617..8889
                     /gene="rpmF"
                     /locus_tag="PK960_02150"
     CDS             8617..8889
                     /gene="rpmF"
                     /locus_tag="PK960_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPD08000.1"
                     /translation="MVQMKKRTKSETRRRRSHHALKAKVLVKCPQCGQLILPHTACSF
                     CGTYKGKPVGQIKGAKTKTAKKASKKEAPTAKPEVKDKGKIKNKVK"
     gene            8893..9369
                     /gene="nusB"
                     /locus_tag="PK960_02155"
     CDS             8893..9369
                     /gene="nusB"
                     /locus_tag="PK960_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPD08001.1"
                     /translation="MANRHLARTLAMQTLFCWDFNGQKEDLDELIEEGFRQFAPEFDD
                     HGFVRDLVKGVVANLPVIDNYIVKYATEWPLDQITPVDRNILRIGIYELALDPNIPAK
                     VAINEAIEVAKTFGGDASGRFVNGVLGAVYKEIPIKSIDKASPIVKDEETGKDEEK"
     gene            9376..10071
                     /gene="rnc"
                     /locus_tag="PK960_02160"
     CDS             9376..10071
                     /gene="rnc"
                     /locus_tag="PK960_02160"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPD08002.1"
                     /translation="MTKQQELANKLGVKFKDDKLLTQALVHRSYLNEHSDWPTGHNER
                     LEFLGDAVLELAVTEYLFLHYPDKSEGELTDWRAALVNAKTLAQIAQEIELDKYLFLS
                     HGEAKEKDSKGHQYILADAMEALLGAIYLDQGWAVAQQVIIKLVLSKLNYILDNKLYL
                     DPKSYFQERAQEVYGSTPHYEVLASSGPDHEKVFEVGLYVDDRLISVGRGMSKQEAQV
                     DAAEKGLKQEGWR"
BASE COUNT         2884 a         1603 c         2289 g         3238 t
ORIGIN      
        1 gatattgttc cgggttttta ttaacccgag ctaattcagc cttatcaaaa agctgaattt
       61 tagctaagat taaattcttt gttttttcag cgcaatgtgg gttatccagt atcgcgctgg
      121 cttcatctaa cgttctacac ccaatagaaa gggtgtgtag ggcttccgcg tcccttgtca
      181 gactcaattc ttgaggtgac atttttcctc cattttttat ttttcccttt gtagggatag
      241 tgtttattta tcactatcta ttaggtttgt caaatctgcc cttaggttgg gtggactaag
      301 tagtctggtt atgttaaatt ataaagagag gagttagttt taagctaaac actatgcgtc
      361 ttttggattc tcatgctcat gttaatttta atgcctttaa ggatgatggc gatgaggtga
      421 ttagaaaatc tttagaagct ggagtcggga tgattttagt tggttcagaa tttcgcactt
      481 ccaatcgggc tattgattat gccaatcatt atgagactgg agtatgggcg gctattggcc
      541 ttcatccctc tcagcttttt gcgactcgag tccagggtga agattatgat tttatgagtc
      601 ggggagagga attggattta gaaaagtatg ataaattagc tgattttagt aaagtggtag
      661 ctattggtga gattggttta gattattacc gcttagcaga tattaatgaa tctcccaagg
      721 ttattaaaga aaagcagcaa cgggttttat ctcaagaact tactttagca atgcatcacc
      781 atttaccagt catcatccat tgtcgtgaag cccatgatga tttactacca ctgcttcaag
      841 atttttatca tgattaccgg caaattttac ctaaagataa accctgggga gtggtacatt
      901 gtttttcagg agacatgggg ctggctcagg aatactttag tctaggtcta gatatttcat
      961 ttaatggtct tattactttt tgttcaacct gggactattt aattcagaaa atgcctttag
     1021 atagattatt aattgaaaca gattgtccct ttttaacacc agtgcctttt agggggcaac
     1081 gtaatgagcc aagtttggtt aagctagtag ctgagcggat agctcaaatt agacatacta
     1141 ccattgagtg cattgctgag gcaaccactg ccaatgcctg tcgtttattt aatattaaat
     1201 ggtaaattat atgaccatag aaattatttt aggagtagga ctagttttag ccgttgctgc
     1261 tttaatttgg ctgatcacta ggcaggagac gggcagtgaa aaaatagtag cccttaatga
     1321 aaaattagaa actcttacta atcaaaataa tgagctaaga gcgttggttg atgaaaaatt
     1381 acgtgagact taccaggcta gccagaatca ataccaaaat acaatgaaca cggtgcaatc
     1441 tattaatcaa ctattaaccg atgtcacaaa acatcttgct gaactccagg ctagtaataa
     1501 acaaatttta ggtttttcgg gccagttgca aaatttacag gatattttgc gcaatcctaa
     1561 acaacgggga gtcttgggtg aatatttttt ggaggaaatt ctcaaaaatg tcttgccgcc
     1621 cgggactttt caaatgcaat atcagtttgc taatggagaa atagtagacg cagttatttt
     1681 tgttaaagat aaaattatcc ccattgattc taagttttct ttagagaatt atgaaaaaat
     1741 tattaatacc agtgatccgg gagagcggct acggttagaa aaattattcc gtcaggattt
     1801 aaaaaataga attgatgaaa ccagcaaata tgtcaggcca gaggtgggga caatggattt
     1861 tgcttttatg tttattcctt ctgaagcaat ttattatgat ttattagtta ataaagtggg
     1921 ggcagttcag attgatacac gagaccttat tgaatatgct ttccgtgata aacatgttat
     1981 tattgtctcg cccactagct ttttagctta tctccaaact gttttacagg gacttaaagc
     2041 cttacaaatt gaagagaatg ctaagattat taaaagcaac gtggaaaagt tgtcccgtca
     2101 tttaagtagc tatgaagatt ttttgcgtcg cttgggaggt agtttaggga cagcagttaa
     2161 tcattataat caagcttatc aagagtttaa aaaagtggac cgtgatgtgg tgaaaattac
     2221 ggagagtgac catcgggttg agccgatggt gttagagagg ccccatgagg aataattatg
     2281 actagccgtt ttgtcgaacg acggcaacaa aatgcttgga ctttagaatc ggctatccga
     2341 gaaacggtag cctatttttc attatttcaa tatccgctta ctctttggga gatatggtcg
     2401 tttttaccag agaaagcaga tttattagct gttaaacggg aggtagacag attggttaaa
     2461 gaagaaaaac tatatgagcg ctggggttat tattttttgc ctggtgaagc gggggcagta
     2521 gattgtcgcg ctgaacgtta tcctataacc attgccaaga ttaaacgggc taagaaagta
     2581 gctaagtggt tgcgttggtt accaggtatt cagtttattg gtttagccaa tgtcatgggt
     2641 tcctataatt tacgctctga tagtgatatt gattttttta tcattactaa gcccggttct
     2701 ttatggtttt gccgtttttg ggctaattta atgatgcaat tggtgggatt acggccgact
     2761 gatgccaaac gaagtaataa aatttgttta agcttttggc tggctagtaa tgatttacgg
     2821 ttggaccgat tcaaattgcc agatgaagat ggctatcctg attggtatta tatttattgg
     2881 ttagcaaatt tacggcctct tgtagaacag gggataaatt accagcagat tattaaaagt
     2941 aatccctggt taaaaactta tttacctaat tggcgtccta gtattattag ccaatctggg
     3001 gggaattttt ggcagagggt agctagtttt ttttctcgat tcggtattgt ccgggccaaa
     3061 atttgggagt ctactttagc ctattggcag cagaagtttt taccagccaa tgctcggact
     3121 ttaatgaata ttgatgaacg ggtagttgtt agtaaagaag ttattaaact tcatattatt
     3181 gaccggcgtg agtattatcg cagtaagttg aggcagaaat tagaggctat agcgcggggc
     3241 gagaaaggga ctatcagacc agatgaaccg gtgcgtcgat taccaccgaa ttggtggttg
     3301 cgactaggaa atattgcagc tactattttg gtgttaattt taccgtggtc aacacgatgg
     3361 attttacatc ccggagaatt aggtggggag ttttgggaat atgggacgat tagtatatat
     3421 cttagcgata ttgtattgct tcttagttgt ttggcagtta agctaggcga agtttcatca
     3481 cctagaatat caaccaaaga ttctagcaaa aaatattatt tagggtggct cagtggccta
     3541 tggctaatta gtgctgttag tttgttggtt aatggggtgt cttggtcact tgttattttt
     3601 tatttgttgc gtcttattat atggagtgga ggctggtatt ttttgctggg tcatcttaag
     3661 tggccttggc ctcggtgggc tactttattt ctagctagcc ttattgttcc tgctctttta
     3721 ggattaggac aatttattgg gcaagatgcc tcaggtaata aatggttggg tctcgctagc
     3781 catcaccccg gcgatttggg agtagcagtg attgaatcca ccaatgcagc tggcgaggtg
     3841 accggccgtt ggcttagagc ctatggcagt tttgatcatc ctaatatttt aggagcgttg
     3901 atggccatag ctattatttt aataatggtt gcaagtcaat attatgagag taatttatct
     3961 cgttttttac tttttgcttc ggtaggatta tggtcgctta cagctctagt tagttggagt
     4021 cggacagctt ggctggcaat tctttttggt tggttaatta gtttaatgct tattgggcgc
     4081 aaggatcgtc accgccgaca atttttggtt gtgtttcttc taggattatt gctgtggtca
     4141 ggtttgtggt tttggccatt taaggactta gggcgtagtc gggtttccgg tgaaccgcgg
     4201 ctggagcaga aatctttagt agagcgcaaa gaaagttatg gtgaggccct gagaattatt
     4261 agggagcatc tttggcttgg tgtaggtcta ggaaattata ctggtttttt aagtagacaa
     4321 aatgaaacta ttggtccagc ttggaattac cagccagttc ataattcttt ccttttaatt
     4381 attagtgaga ttggtcttct tggttttgtc tggttagcgg tgggactagt aatgctttta
     4441 cgctcagcca gtaaacgctt agagattatt tggccacttg gtgttagttt tatttggctt
     4501 ttactaatgg atcattggtg gtggagtcta catagcggga tagtatattt ttggctagtt
     4561 atttcaagcc tttattggat tagccgtcaa cctagaaatt tgctataatg taattagttt
     4621 ttccctataa ttttaatcat taattttatt acttaggcct atgaaaaaaa tttatttatc
     4681 ctcgttttta atagtagctc taggcctggt tggttggcag ctgggtcatc acttgttacc
     4741 aactgctggg gcggccccta gccctagttg tactattatg attacaaaca ttgatcctaa
     4801 acaatacagt cccgataggg ttgacacaat tttatatgat tctggattaa ttactaaagg
     4861 tgcatttgta gttgcttcta aaaatggagc cttgccgttt caaactcagg cagctgctca
     4921 aacaaccatt gattcagcta ttagtcaaat aattaaggag gtttgcggag gcaactgtaa
     4981 tcctaatgct ttgacaatgg acattcattg tcccagctct agttgcccta caagtgttct
     5041 tagttttact gtatttgatc ctaaacaata taatcccgat acggttgaca caattttata
     5101 tgattttgga ttaattacta aaggtgcatt tgtagttgct tctaaaaatg gagccttgcc
     5161 gtttcaaact caggcagctg ctcaaacaac cattgattca gctattagtc aaataattaa
     5221 ggaggtttgc ggaggcaagt gtaatcctca agccctacag atagtgcccc aatgtgtgac
     5281 agaaagtccg gttaatgtgg cgagacaggc ctatgaacaa cggctttcta atatgaatga
     5341 ttgtcaaaaa caagtttttc aagctatgca ggctaataat gagcagtatc tagctaaggt
     5401 aaagaaaaat aatcaacaat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 caacaatcta ttaagcttaa tcaagaattt gctgcttgtg ccaagaaata gtctatgctt
     5581 atttttaagc acattctgag cgtgatgaat ttagataagc cgctggttat ttttgattta
     5641 gaaacaactg gtttagcttt agcttatgac cggattattg aattagcgtt tgttaagata
     5701 atgcccaatg ggcgagtgat tgaaaaagac atttttttta atcccgaaat tgatgtaccc
     5761 agtgaagtaa cagctattaa tggactgact aatgccgatt tggccgataa acctttattt
     5821 cgtgatcatg cccaggagat ttgggatata ttttatgatt gttattatgg tggttttaat
     5881 gtggttaatt ttgatttgcc aatgttgcgg cgggaatttt taagggctgg ctatgatttt
     5941 aattatacta aaaagcaggt tattgatagt aaagttattt atcatgccat ggagccgaga
     6001 gatttagcag cagcttataa atattattgt caaaaagaat tagccaacgc tcatagtgct
     6061 ttagctgatg tccgggctag tgcagaaatt ttagatcaac agttggctaa atatgattat
     6121 ggactcattc ggcagattag cgaagcgccc gatgaccgtt atgtagatga agagaaaaga
     6181 ttttattggc gaagtggcga agcttatctt aatttttcta agtataaaga taggtcacta
     6241 gctgaaatag ctaaagttga tcctggtttt ttaaggtgga tgatggaagc cgatttttct
     6301 cctgagacta aacaaattgt agaacgagcc ctggaagggg aatttcctaa aaagcaatct
     6361 caagatagta catctagcaa tgatatagta gcgccagcta aaaagtcaat gagtggtttt
     6421 gtggaagagc caaaattatt ttaacatgag caaaaattta aaaaaaatat ctaaagatgg
     6481 cctattagcc gctgatttgg cttcagatag tgatgaagct ctagatttaa gtttacggcc
     6541 tcagactctt agtgaatatg tgggtcagcc agcaattcat cagagtctag agattttttt
     6601 gcaggcagct cagtctcgcg gtgaaaacat tgaacacgtt ttgctttatg gtgcgccagg
     6661 cctgggaaaa actactttag cccatgttat tgccaatgag ctgaaggcta atttgcgggt
     6721 aacatctggt ccagccattg aaaaggctgg agatttagca gcgattttaa ctaatttgca
     6781 ggatggggat ttactattta ttgacgagat tcatcgttta ccccgtagtg tagaagagat
     6841 tctttatccg gcaatggaag atggagcgct agatatagtt ttaggtaagg ggccgtcagc
     6901 gcgtatttta cgtctagatt tgccgcgttt tagcttaatt ggggcgacta ctaaaattag
     6961 tttgctttcc gcccccttaa gagatagatt cggtatggtt taccggctgg atttttatgg
     7021 ggttgaggat ttagagaaga taattaaacg cagtgctaag attttacaaa ttgaattaga
     7081 accttcagct agtcatttaa tagccgaaag ggctagacgg acaccgcgaa ttgctaatcg
     7141 cttgttaaaa agagtaagag attattccca ggtcaagggg agtggggcta ttactgagga
     7201 tatttgtcgt caagcttttg aactactcgc cattgatgat cttggtttag ataatatgga
     7261 ccgtctgtta ttagaaacca ttatagataa atttggaggt ggtccggtgg gtcttaatac
     7321 tttagctgcc tctacaggag aagaattaat aactatcgaa gatatttatg agccctattt
     7381 actgcagtta ggctttttag aaagaacacc acggggccgt aaagttacag tgaaagcata
     7441 taaacatctg aatcgcgtgc cccctgatag tctattttag tatggattta tctgattttg
     7501 attatttcct tccgccggaa ttaattgctc aggccccagc tgagccacgt gacagggcta
     7561 aactcatggt agttaatcgg caaactggca cttggcagac gactattttt tctaatctgc
     7621 cccaattttt gcaaccatca gatattttga ttgtcaataa ttctaaagtt ttgccagcca
     7681 gattgatagg ccataaagcg ggaacaggtg gaaaaataga ggtactactc gagcataaat
     7741 tagtaagtcc cagtggccaa gagagttggc gttgtcttat tggtggtaat cggcctaagg
     7801 taggacttat tattaatttt ccccaggatt taacaggaca aattactgcg gaagaggggg
     7861 atggagtttt tacggtagaa tttaataaga gcggtagtga tttttggtct gccgtatatg
     7921 ctattgggga gatgcctttg ccaccatata ttaaagctga caagacaaca attaaggagg
     7981 ccgttaaaga ttaccagaca gtttatgcta aagataataa agttggctca gtagctgccc
     8041 ctactgcagg tttgcatttt actaaagagt tgttgaaacg tattaaggca atgggagtag
     8101 aaattgttga agttacgctt catgtaggat tagggacttt tgagccggta agagatgaga
     8161 atattaaaga acaacgactc catgaagagt tagtggagat cggggctcaa aaaattaatc
     8221 aactaataaa atatcatcaa gccggacggc gcttaattgc cgttgggact acctcagtaa
     8281 gagtattgga gggggtagcg gctcacttta tagatacctt agctacagat gattggttgg
     8341 cacccattga tttatttatc tctcccggct ttgaatttaa actgttagat ggcttaataa
     8401 ccaattttca tctgcctaaa tccagtttaa taatgttagt ttctgcttgg gctggcactg
     8461 atttaataaa aaaaacatat gcagcggcta ttgccgagca ttaccgtttt tttagttatg
     8521 gtgatgccat gtttttagta taattctgat ttctagattg acgaactttt tagttttaat
     8581 tatactgaga gcactaagct agttattatt atccctatgg ttcaaatgaa gaaacgcact
     8641 aaaagtgaaa ctcggcgacg tcgttcccat catgctttaa aagccaaggt tttggttaaa
     8701 tgtccgcagt gtggtcagtt aattttacca catactgctt gttctttttg tgggacatat
     8761 aaaggcaagc cggtaggaca gattaagggg gctaagacta aaaccgcgaa aaaagcttct
     8821 aaaaaggaag ctcctacggc caagcccgaa gtcaaagata aaggtaaaat taaaaataaa
     8881 gttaaataat gtatggccaa tcgtcattta gcgagaactc tggccatgca aactttattt
     8941 tgctgggatt ttaatggcca gaaagaagat ttagatgaat taattgaaga gggttttcgg
     9001 caatttgctc ccgagtttga tgaccatggt tttgtccgag atttagtcaa gggagtggta
     9061 gctaatttgc ccgtcataga caattatatt gttaaatatg cgactgaatg gcccctggac
     9121 caaattactc ccgtagaccg taatattttg cggattggta tttatgaact agcccttgac
     9181 cctaatatac cagctaaagt agctattaat gaggctattg aagtagctaa gacttttggc
     9241 ggtgatgctt cagggcgttt tgttaatgga gtgttgggag cagtttataa agaaataccc
     9301 attaagtcta tagacaaggc gtctcccata gttaaagatg aggaaactgg taaagatgaa
     9361 gaaaagtgag aggatatgac aaaacagcaa gaattagcta ataaattagg agttaagttt
     9421 aaagacgata aattattgac tcaggcttta gtgcatcgtt cttatcttaa tgagcatagt
     9481 gattggccga caggccataa tgagcgactg gaatttttgg gtgatgctgt gttggaatta
     9541 gcagttactg aatatttatt tttacattat ccggataagt ccgaagggga attaactgac
     9601 tggcgggctg ctttagttaa tgctaaaact ttagcccaaa ttgcccaaga gattgaattg
     9661 gataaatatt tatttttgtc tcacggtgaa gctaaagaga aagattctaa ggggcatcag
     9721 tatattttag ctgatgctat ggaggctctt ctaggggcta tttatttaga ccaaggatgg
     9781 gcagtagctc agcaggtgat cattaaatta gttttatcta aactcaatta tattttagac
     9841 aataagttat atttagatcc gaagtcatat tttcaggagc gagctcagga agtctatggt
     9901 tccacgccgc attatgaagt attagcttct agtggtccag accatgagaa ggtttttgaa
     9961 gttggtcttt atgttgatga ccgactaatt agtgttggcc gtggtatgtc taagcaggaa
    10021 gctcaagttg atgctgctga gaagggctta aagcaggaag ggtggcgtta acttggtcgg
    10081 cgtttgataa ttattattat taaaccacta acaa
//
LOCUS       DAOOIS010000016        10067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_5962_length_10067_cov_41.721534, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000016 DAOOIS010000000
VERSION     DAOOIS010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10067
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5962_length_10067_cov_41.721534"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PK960_02165"
     CDS             complement(<1..296)
                     /locus_tag="PK960_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HPD08003.1"
                     /translation="MPIIKLTSHNLDQRLKDIASVILGGGVVLLPTDTIYGLSALASN
                     ETAANRILSLKERSGGKPFICLINSLEMAEDYAIISPKCRKYLLASWPGPVSVI"
     gene            complement(300..815)
                     /locus_tag="PK960_02170"
     CDS             complement(300..815)
                     /locus_tag="PK960_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPD08004.1"
                     /translation="MLAVDIFFIVILGLAFIIGLRRGLLRIISSLVGVILGAWLSSHY
                     YLLVFDWLVKQFGELRLINKIVIFIVLFFLLSNLIAWILTLISKLLETVTVIPLMKSA
                     NYLLGGVLNLAKSALAWSLIVLFLSRYLPATTPLGAQLSRSIIAPYLLMIGKVLWPLL
                     PVVFKEMQALW"
     gene            complement(820..2250)
                     /gene="metG"
                     /locus_tag="PK960_02175"
     CDS             complement(820..2250)
                     /gene="metG"
                     /locus_tag="PK960_02175"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPD08005.1"
                     /translation="MAKFYITTTLPYINAEPHLGFALEIVRADALARAHRLSGDEVFF
                     NTGVDEHGLKVWRKARELKMEPQAYCDQSALSFKGLKDLLNLSYDNFIRTTDPHHEAA
                     AQEFWRRCAAHGDIYKQHYKIKYCVGCEMEKTDSELVNGHCPLHPDQELELIDEENYF
                     FRLSRYQKRLLELYDSRPDFVVPTARLQEIKNFALRGLRDFSISRLASKLPWGVPVPD
                     DASQVMYVWFDALVNYISALGWPEDSDNFLAWWPGVQIAGKDNLRPQALMWQAMLMSA
                     GLEPSRQIFIDGFITAGGQKMSKSSGNIIGPQELTKRFGIDAVRYYLLAQVNDDDDGD
                     YSLERFEEVYEADLANGLGNLFSRLGNLAWRYNLTPVYPVKDGELYKKIIQLLYQWHF
                     NQAIGEIWSVLRNLDKTLTQEEPWKKETEAAQDSLRPLIQQLVNAGYCLSIFLPQSAG
                     SILDFFGHKAQGKPEPLFPRLNRLKI"
     gene            complement(2294..3295)
                     /gene="rlmN"
                     /locus_tag="PK960_02180"
     CDS             complement(2294..3295)
                     /gene="rlmN"
                     /locus_tag="PK960_02180"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPD08006.1"
                     /translation="MNWSAWEHFLDTQPAFRQRQLNHAVFQELVTDWSAVSVLPLKER
                     QWLEVNCPLDINYTLSGQDADPAQKARITLRDGLIIETVVIHHETGRQTVCLSSQAGC
                     PLGCAFCQTGQQGFRRNLKAGEIVEQALLWQRLLAPQGKHIHNLVFMGMGEPLLNLAE
                     VIRAIEWLHNSMALDIGWRHIAISTVGLRPQLKDLLNYGEQIKIAWSLHAPSDNLRNK
                     LMPVNHHHSLMALKPIFRDYVVRTNRRLMVEYLLLDHFNDSLEEAEELAIFLADFPKN
                     LLLINLLNYNPGVGNFYPSSGSAREAFAVFLEKRGYKVTTRLSLGQDILGACGQLSW"
     gene            3391..5907
                     /locus_tag="PK960_02185"
     CDS             3391..5907
                     /locus_tag="PK960_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPD08007.1"
                     /translation="MTKHKNNKTRQSSNLNYLSVGKGGLSAEAKKSLAAIILVLLAAI
                     SLISLIQPEGIIGQYLSQGICQVLGWGRWFLPIFLFALAVFIYFRERLQITTIKFVGW
                     GLLFISLQGLFHFFLNQENWLSAVPEGAGGGYVGYGITWAITKLVGYWSGLILIIALL
                     IISLIFIFNANLSSLLGRQSIFNKFIFAPLADGWKYIKSLFSKHPAESDLSPEEELST
                     VYMPESEEKKPKHLSGVTASPIINQPNIDDQNQPNAQEKINKSHTNKIDLPLDLLDRQ
                     ANKPQSGDLVIGAQRIKTTLENFGIPVNIEEVTVGPTITQYAIKPAEGIRLEKITGLA
                     SNLAMALAAPHPIRIEAPIPGKSLVGIEVPNEVIATVGLREILDSPDFRQRKNNMMIA
                     LGKDVTGKIWLEDLTAMPHLLIAGATGSGKSVMLNCIILSLLYENSPEDLRLIMVDPK
                     RVELSVYNGIPHLLTPVVNEASKTINALGWTLNEMSRRLEMFQHYHAKDINSFNEQLP
                     DQKLPRIVFVIDELADLMMMSRKEVEASIIRLAQISRAAGIHLVLATQRPSVDIITGV
                     IKGNMPARIAFTVASQVDSRTILDMAGAEKLMGKGDLLFSSPRLTKPVRLQGAFVSEN
                     EIKRVVRYIKARSDKPQYINDVVEKHSVPDLPNGGILNNPLSESEDDLLEKAKEAVIK
                     SGRASATYLQRMFKIGYPRAASLLDELEELGVIGPINGAKPREIYISLEEYEARKQMA
                     TSSQPLHNPVTSEPPSQFLPLDDVWSEDKEANSQDENTPFSASNDNDEDQDEMPLDKE
                     KILSDIYSDVDEDEENRAEDGPQGHKRFDDDEDQEKYFSQ"
     gene            5920..6342
                     /locus_tag="PK960_02190"
     CDS             5920..6342
                     /locus_tag="PK960_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPD08008.1"
                     /translation="MPHHNRPAQTLKDQDNSKSKETNDWLPLGSHEGQLSLDIYQTDK
                     DLIIKSTIAGANPNDIQIYLKNDLLTIRGKRNLPADVADADFLFRECYWGPFSRSIIL
                     PVEVQPDKIKASMDNGVLTIVLPKAPQAKQIIIKIKNQ"
     gene            6345..6566
                     /locus_tag="PK960_02195"
     CDS             6345..6566
                     /locus_tag="PK960_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08009.1"
                     /translation="MHCQLTIIKLDDHQAVLINKENQQFIWPANQLPPESRVGTVLTC
                     ALVNANKLDQDDQALAKAILNELLKKSST"
     gene            6570..8456
                     /locus_tag="PK960_02200"
     CDS             6570..8456
                     /locus_tag="PK960_02200"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HPD08010.1"
                     /translation="MPVPRLSGKKFRVKKTIWPLTVSLGLICLILLGFSLYGLEYYRH
                     RFYPHTLIGNLDLSGLTPAEASELIQNHWNQLVSKGWTFEALGQSMVIHQVGGMAEGA
                     PLLFSLDIEDTLNKAWGGQHHNHPTIASFIADYFAKHQTTASVMINELLVRQQLEANF
                     QPLITPSKPATIEVQPDGSYQIKPEIIGESIDYEAGISEFKKQLQNLKNEPIILTVKP
                     DQPTITQSAIGDLTGPINQYLTLAPITLQLATTTWTINRNTLAKWLTLELVDNQITAS
                     ISSSSVKDYLTKLAPEINQEPQGGQFKRNGNRLEQFKPGKNGRELNIEVSTNAIIQAL
                     KQGINNIELTVNDIPSPLSDTDPASLGIIEIIGIGTSDFSGSPPNRIHNIKVGTAKVS
                     GQLVAPGEEFSLVKALGEVSAATGYKPELVIKENKTVPEYGGGLCQVATTIFRAALAS
                     GLPITERQNHSYRVSYYEPAGTDATVYSPKPDLRFINDTGHYILLLGEVSGHTLSFTV
                     WGTKDGRKIIQTKPVIYNIVKPAPRKLIETEDLEPGKLKCIEQAHNGADTYFDYIVTY
                     PNGETKSKRFQSHYVPWQEVCLIGKEKGEESQTTTPSLVSSTDSIFTSTTGNIATTSA
                     TTTN"
     gene            complement(8746..>10067)
                     /locus_tag="PK960_02205"
     CDS             complement(8746..>10067)
                     /locus_tag="PK960_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08011.1"
                     /translation="GQVEKVDRIIKQDKLLVELPKDTKMMVCMAVGAVYESDNIYRTL
                     SLYSHQSEEVLAKTVFVLNVNWLDQDEQDPQKLAQIKKTIEEVERIKKDFPQLKIAVF
                     QERYVKEILVAERGMNIHGQFIKDVHDVALRSLQLSGIDHEVYLLSNDADCQGMSDSY
                     LSKLLEQAEKESEKDAFLGRIEWDTKRWPDYPGFHLSTRFMQFLDLVSRHPGNKRIKH
                     IPTSGANSMVKASTLAAIGGIDPGNELGVGADINVGRRIKYARGVDTEPIGYNHVAWL
                     ETNGDRAFNYYAYGESIVAMWSDFARKGYKGRKERGVSDLGSRHYYEGVKFSKSKFRP
                     NIPKLKLDQQGNFVPEDLERDWHDIVVRFEYQINSGLWAYFQDDFELWQRALNFTFGN
                     IRPPRWEIQDGQVKITKVGQSWLKQQLKSFAAKNSQEILYQRGGHRA"
BASE COUNT         3263 a         2259 c         1733 g         2812 t
ORIGIN      
        1 aaaataacag acaccgggcc gggccacgag gctaacaaat acttgcgaca tttagggcta
       61 ataatagcat aatcttcggc catctctaaa ctattgatta aacaaataaa gggcttgccg
      121 ccagaacgct cttttagaga caaaatacgg ttagccgcag tttcgttact agctaacgca
      181 ctcaaaccat aaattgtatc agttggcaga agcactactc caccccccaa aataacacta
      241 gctatatctt ttaaccgctg atctaaatta tgagaagtta atttaatgat gggcataatt
      301 taccataacg cctgcatctc cttaaagaca actggcagca agggccataa aactttacca
      361 atcattagta ggtacggagc aataattgaa cggcttaact gcgcccctaa cggagtagtc
      421 gctggtaaat agcggcttaa aaaaagaaca attagcgacc aggctaaagc tgatttagct
      481 aaattaagca ccccacccaa aagataatta gcacttttca tcaaaggaat gacggtaacg
      541 gtctctagca atttactaat tagcgttaaa atccaagcaa ttaaattact taataaaaag
      601 aaaagaacaa taaaaataac aattttatta attaaccgca actcgccaaa ctgtttaact
      661 aaccaatcaa aaaccaggag ataataatgg cttgacagcc aagctcctag aattacccca
      721 acaagggagc taataattct taaaagtcca cgccgtaaac caataatgaa agctaaaccc
      781 aaaataacaa taaaaaatat gtctactgct agcatataat tatattttta aacggttaag
      841 acgaggaaat agtggctctg gcttgccttg ggctttgtga ccaaaaaaat caagaatgct
      901 accagctgac tgtggcaaaa agatagataa gcaatagcca gcatttacta attgctgaat
      961 taacggtcta agagagtcct gagccgcttc tgtctccttt ttccacggct cctcttgggt
     1021 taaagtttta tctagattac gtagtactga ccaaatctca ccgatggctt ggttaaaatg
     1081 ccactgataa agtaactgaa taattttttt atacaactca ccatccttca ccggataaac
     1141 cggagtcaga ttataacgcc aagctagatt acctaaacga gaaaataaat tacctaaccc
     1201 attggctaaa tcagcttcat aaacctcctc aaatcgctct agcgaataat ccccatcgtc
     1261 atcatcatta acctgagcca gtaaatagta acgcactgca tcaattccaa aacgcttggt
     1321 taattcttga ggtccgataa tattacctga actcttgctc attttttgac cacccgcagt
     1381 aataaagcca tcaataaaaa tctgacgact tggttctagg ccagccgaca ttaacattgc
     1441 ctgccacatt aatgcttgag gccgtagatt atctttaccg gcaatctgaa ctcctggcca
     1501 ccaggctaaa aaattatcac tatcctcggg ccagcctaaa gcagaaatat aattaactaa
     1561 agcatcaaac caaacataca ttacctgact agcatcatcc ggcactggta ccccccacgg
     1621 cagtttactt gctaaccggg aaatactaaa atcccgcaaa ccccttaaag caaaattctt
     1681 aatttcctgg agtcgtgccg taggcaccac aaaatcagga cgagagtcat aaagctctaa
     1741 caatcgtttt tgatatctag aaagccggaa aaaataattt tcttcatcaa tgagctctaa
     1801 ctcctggtct ggatgcaacg gacaatgacc attcaccaat tctgagtcag ttttttccat
     1861 ctcacaacca acacaatatt ttattttata atgttgttta taaatatcac catgagcggc
     1921 acaacgacgc caaaactctt gagccgcagc ttcgtggtgc gggtcagtgg tccgaataaa
     1981 attatcatag ctaagattta ataaatcttt gagcccctta aaactaagag ctgattggtc
     2041 acaataagct tgtggctcca ttttgagctc tctcgcttta cgccatactt ttaacccatg
     2101 ctcatccaca ccggtattaa aaaagacttc atcgccactc aaacgatgtg cccgagctaa
     2161 agcatcggcc ctgacaattt ccaaagcaaa acctaaatgc ggttcagcat taatataagg
     2221 taaagtagta gtaatataaa acttagccat agtaattaaa ttttatcatc acttcttagc
     2281 aaaagcaatg gaatcaccaa gatagttgcc cacaagctcc caaaatatcc tgtcctaaac
     2341 tcagccgagt agttacttta tagcctctct tttctaagaa tacagcaaaa gcctcgcgag
     2401 ctgacccaga gctaggataa aaattaccca caccaggatt atagttaagc aaattaataa
     2461 ggagtaaatt cttaggaaaa tcagccaaaa aaattgctaa ttcttcagct tcttctaaag
     2521 aatcgttaaa atggtcaaga agtaaatatt caaccatcaa acgccgatta gtccttacaa
     2581 cataatctcg aaagattggt tttaatgcca taagactatg atgatgatta actggcatta
     2641 acttattccg taaattatcg cttggggcat gtaaagacca agctatctta atctgttcgc
     2701 cataattcaa taaatctttt aactgaggcc ttaaaccaac agtagagata gctatatggc
     2761 gccagccaat atctagggcc atagaattat gcaaccactc tattgcccta ataacttcgg
     2821 ctaaattaag caatggttct cccatcccca taaaaactaa attatgaata tgctttccct
     2881 gcggagctaa taatctctgc cataataaag cttgctccac tatctcgcca gccttaagat
     2941 tgcgcctaaa cccctgctga ccagtctgac aaaaagcaca acccaaagga caacctgctt
     3001 gtgaagacag acaaactgtc tgccgtccag tctcgtgatg tattactact gtctcaatta
     3061 ttaaaccatc ccttaaagta atccgggctt tctgcgccgg atcagcatct tgaccactca
     3121 aggtataatt gatatctaaa ggacaattga cctccagcca ctgtcgctct tttaacggca
     3181 aaaccgacac ggccgaccaa tcagtgacta actcttgaaa aacagcatga ttaagctgcc
     3241 tctgccgaaa ggctggctga gtatctaaaa aatgctccca agctgaccaa ttcataaata
     3301 aaaatagttg ttgaaattct ttactaatgg tatactttga ttgtttataa agcaagccct
     3361 gccgctattt tggcatcttc attactaatt atgaccaagc acaaaaataa taaaactcgc
     3421 caatcatcta atctaaatta tttatcagta ggtaaaggtg gcctaagcgc tgaggctaaa
     3481 aaaagtttag ccgctattat tttagtgtta ttagcagcta ttagcctcat tagcctcatc
     3541 caacccgaag gaattattgg ccaatattta tcccaaggca tctgccaagt tctcggctgg
     3601 gggcgatggt ttttgcccat cttcttattt gctctagccg tctttattta cttccgtgaa
     3661 cggctgcaaa ttactactat caaattcgtt ggctggggct tattatttat tagtctccag
     3721 ggcctttttc atttttttct taatcaagaa aattggctaa gtgccgtccc cgaaggagca
     3781 ggcggtggct atgttggcta tggtattact tgggctatca ctaaattagt aggctattgg
     3841 tctggcttaa ttttaattat tgctctcctt attattagtc ttattttcat ttttaatgcc
     3901 aatctaagct ctctcttggg ccgacagagt atctttaata aatttatctt tgctccttta
     3961 gctgatggct ggaaatatat aaaaagtcta ttcagcaaac atccagctga gagtgattta
     4021 tcccccgaag aagaattgtc caccgtatat atgccagaat cagaagaaaa aaaacctaaa
     4081 catttgagtg gggttactgc ctcgccaatt attaatcagc ctaacataga tgatcaaaac
     4141 caacccaatg cccaagaaaa aataaataaa agtcacacta ataaaattga tttacccctt
     4201 gatttattag accgtcaggc taataaacca caaagtggtg atctggttat tggggcccaa
     4261 cgcattaaaa ctactctaga aaattttggc attccagtaa atatcgagga ggtaacggta
     4321 gggccaacaa ttactcagta tgccattaaa ccagcagaag gtattcgtct ggaaaaaatt
     4381 accggcctag ctagcaatct agctatggcc ctagctgccc cccaccctat tcgtattgaa
     4441 gcccctattc ctggtaaatc cctagtgggg attgaagtgc ccaatgaagt tattgccacg
     4501 gtaggattaa gagaaatatt agatagccct gatttccgcc aacgcaaaaa taatatgatg
     4561 attgccttag gcaaagacgt aacaggcaaa atttggctgg aagaccttac cgctatgccc
     4621 cacttattaa ttgctggagc gactggctcg ggaaaatcag tgatgctcaa ttgtatcatc
     4681 ttaagtcttt tatatgaaaa cagtcccgaa gatttacgcc tcattatggt tgatcctaaa
     4741 cgagttgagc ttagcgtcta taatggcatc ccccatctct taactccggt agttaacgag
     4801 gccagcaaaa ctattaatgc cctaggttgg actttaaatg aaatgagtcg tcgtctagaa
     4861 atgtttcagc attatcatgc taaagatatt aatagtttta acgagcaact tccagaccaa
     4921 aaattaccac gtattgtttt tgttattgat gaattagctg atctcatgat gatgtcccgt
     4981 aaggaagtgg aagcgagcat tattcgctta gcccaaatat ctcgagccgc tggtatccat
     5041 ttagtcttag ccacccaacg tcccagtgtg gatattatta ccggcgttat taaaggtaat
     5101 atgccagccc gcattgcttt tactgtagcc tctcaagtcg attcacgcac tattttagat
     5161 atggccgggg cagaaaaatt aatgggcaag ggagatctcc tctttagttc cccacggctt
     5221 actaaaccgg tccggctgca aggagccttt gtcagcgaaa atgaaattaa acgagtagtc
     5281 cgctatatta aagctcgcag tgataaacca caatatatta atgatgtcgt agaaaaacat
     5341 agcgtacccg acctccccaa tgggggtata ctcaataatc ctctcagtga aagtgaggat
     5401 gatttattag aaaaagctaa agaagcagtt attaaaagtg gacgagcttc agcgacctac
     5461 cttcaaagga tgtttaaaat tggttatccc cgggcggcca gtcttttaga tgagctagaa
     5521 gaattagggg tcattggacc gattaatggg gctaaacctc gagaaattta tattagcctc
     5581 gaagaatatg aagcccgcaa acaaatggct accagcagtc aaccacttca caatcccgta
     5641 actagtgaac caccttccca gtttcttccc ctagatgacg tctggtccga agataaagaa
     5701 gctaacagtc aagacgagaa tacccccttt tcggctagta atgataatga tgaggaccaa
     5761 gatgaaatgc cattagataa agagaaaatt ctatccgata tttatagcga cgttgacgaa
     5821 gatgaagaaa acagggccga ggatggcccc caaggtcata aaagatttga tgatgacgaa
     5881 gaccaagaaa aatattttag ccaataaatt aacaattata tgcctcatca caatcgtccg
     5941 gcccaaacac tcaaggacca agacaactct aaatctaaag agactaatga ttggctgccc
     6001 cttggcagcc atgagggaca gctttcttta gatatttatc agacagataa agatttaatc
     6061 attaaatcta ctattgctgg cgctaatcct aatgatattc agatctatct taaaaatgac
     6121 ctattaacta ttcgcggcaa acggaaccta cccgctgatg ttgctgatgc tgatttcctt
     6181 tttcgagaat gctattgggg gccattttcc cgttccatta ttctcccggt ggaggtccaa
     6241 cctgataaaa ttaaagcctc tatggataat ggcgtactaa caattgtcct gcccaaagct
     6301 cctcaggcta aacagattat cattaaaatt aaaaatcaat aaccatgcac tgccaattga
     6361 ccataattaa gcttgacgat caccaagcgg tcctgattaa caaagaaaat caacaattta
     6421 tctggcccgc taatcaatta ccacccgaat ctcgagtagg tacagttctt acctgtgctc
     6481 tagttaatgc taataaatta gaccaagacg accaggcact agctaaggct atcctcaatg
     6541 aactcctgaa aaaatcatcc acctaaatta tgcctgtccc ccgtctaagt ggtaaaaaat
     6601 ttagggtaaa aaaaactatc tggccactca ccgttagcct tggccttata tgtcttatac
     6661 tcttgggttt tagcctctat gggctagaat attatcgcca ccgcttctat ccccacacct
     6721 tgattggcaa tcttgatctt tcaggtctta ctcctgctga agccagtgag cttattcaaa
     6781 atcactggaa tcaattagta agtaaaggct ggacctttga ggccctgggt cagtctatgg
     6841 taattcatca agtgggagga atggctgaag gagctccctt gttattttct ttagatatag
     6901 aagacacctt aaataaggcc tggggggggc aacaccataa tcacccaacc attgctagct
     6961 ttattgctga ttattttgcc aaacatcaaa ctacggcctc agtaatgatt aatgaactgc
     7021 tcgtcaggca acaattagaa gccaattttc aacccctaat cacccctagt aaaccggcaa
     7081 ccatcgaagt acaacccgat ggtagttatc aaattaaacc agaaataata ggcgaaagta
     7141 tagattatga agctggtata agtgaattca agaagcaact acaaaattta aaaaatgagc
     7201 caattatttt aacagttaaa ccagaccaac ccactattac tcaatccgct attggtgatc
     7261 ttactggacc aattaatcaa tatctgactt tagccccaat tactctacag ctagcgacta
     7321 ctacctggac tattaatcgc aataccctag ccaaatggct cacccttgag ctagtagata
     7381 atcaaatcac tgctagtata tctagtagca gtgttaaaga ttatcttaca aaattagccc
     7441 ctgaaattaa tcaagaaccc cagggaggac aatttaagcg caatggcaat cgtctggaac
     7501 aatttaaacc agggaaaaat ggacgtgaac ttaatatcga agtgagtact aatgctatta
     7561 ttcaagctct aaagcaaggt attaataata ttgaacttac ggttaatgat ataccaagcc
     7621 ccctcagtga tactgacccg gctagtttgg gcatcatcga gataattggc attggtactt
     7681 cagatttttc tggctcgccg cctaatcgta tccacaatat taaggttgga acagccaaag
     7741 tcagtggtca attagtagcc ccaggggaag aattttcttt ggtcaaggcc ctgggcgaag
     7801 taagcgctgc cactggatat aaaccagagt tagtaattaa agaaaataaa actgtccccg
     7861 aatatggtgg agggctatgt caagtagcaa ccaccatttt ccgcgctgcc ctagctagcg
     7921 gcttgccaat aaccgaaaga caaaatcatt cttaccgtgt tagttattat gaaccggccg
     7981 gcactgatgc taccgtttat agccctaaac cagatttacg ttttataaac gataccggac
     8041 actatatttt acttttaggg gaagtaagcg gtcataccct atcttttact gtctggggca
     8101 ctaaagatgg acgaaaaatt atccaaacta aacctgttat ctataatatt gtaaagccag
     8161 ccccacgaaa acttattgaa actgaagact tagaaccagg caaattaaaa tgcatcgagc
     8221 aggcccataa tggagccgat acttattttg actatatagt aacctatcct aatggtgaaa
     8281 ctaaatccaa gcgtttccaa tcgcactatg tcccctggca agaggtttgc ctaattggta
     8341 aagagaaggg ggaagaaagc caaaccacca caccctcgct agtgtccagt actgattcta
     8401 tttttacttc tactactggt aatattgcca ctacttcagc aaccactact aactaaaact
     8461 cataataact ttaataaaaa aacgagccct aggcccgtct ttatattttt acaaattaac
     8521 tctggtaggg taggcaagaa acttttctct aggacagacc cagtctcaac caggtacagc
     8581 ttatagaaaa atccccatgc ttgtcgtgcc taaccccgcc agagctaatt tgttttacta
     8641 attcttttta aataactaga tatcatagca taaaataaaa atcttgtcaa gggtaaatcc
     8701 ctgacctgta aatcagcagt cctctaaaag taaatatcaa gtcaattaag ctcgatggcc
     8761 acctcgttga tacaatattt cttgtgaatt tttagctgca aatgatttta attgttgttt
     8821 taaccaactt tgcccgactt tagttatttt aacctgacca tcttgaatct cccaacgagg
     8881 tggccggata ttcccaaaag taaaatttaa agctcgctgc cataattcaa aatcatcttg
     8941 aaaataagcc catagcccag aatttatctg atattcaaat ctgacaacaa tatcatgcca
     9001 atctcgttcc aaatcttcag gtacaaaatt accttgctga tctaatttta atttaggaat
     9061 gtttggtctg aatttacttt tgctaaattt caccccctca taataatgcc tgctccccaa
     9121 gtcactgact cctcgttctt ttcgcccttt ataacccttt cgagcaaaat cggaccacat
     9181 tgctactatt gattctccat aagcataata attaaaagct ctatctccat tagtttctag
     9241 ccacgcaaca tgattataac caatgggctc agtatcaacc ccacgggcat acttaatacg
     9301 tcgccccaca ttaatatctg cgcccactcc taactcatta ccaggatcaa ttcccccaat
     9361 agccgccaag gtagaggcct ttaccatcga atttgctccc gaagtgggaa tatgcttaat
     9421 acgtttatta ccaggatgcc ggctaactaa atctaaaaat tgcataaacc ttgtcgataa
     9481 atggaagccg ggataatctg gccagcgttt ggtgtcccat tctatacgac ccaagaaggc
     9541 atccttctct gattcttttt cggcttgttc taacaattta cttaaatagc tatctgacat
     9601 accttgacaa tcagcatcat tagataacag ataaacctca tgatcaatac ctgataattg
     9661 taaagacctt agagctacat cgtgcacatc tttaataaat tgaccatgga tattcatacc
     9721 acgttcagcc accaaaatct ccttaacata acgttcttga aagacagcaa tttttaattg
     9781 aggaaaatct tttttgattc tttctacttc ctcaatagtc tttttaattt gggctaattt
     9841 ctgaggatcc tgctcatctt gatctaacca attaacattt aaaacaaata cagttttagc
     9901 taaaacttct tcagattgat gcgaatataa agataacgtc ctataaatat tatccgattc
     9961 ataaactgcc cccacggcca tacataccat cattttagta tctttaggta gttcaactaa
    10021 taatttatct tgtttaataa ttctatcaac tttttctact tgccctg
//
LOCUS       DAOOIS010000017         9679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_6237_length_9679_cov_54.071696, whole genome shotgun sequence.
ACCESSION   DAOOIS010000017 DAOOIS010000000
VERSION     DAOOIS010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9679
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6237_length_9679_cov_54.071696"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1343
                     /locus_tag="PK960_02210"
     CDS             <1..>1343
                     /locus_tag="PK960_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPD08012.1"
                     /translation="FILKLIGYTLLIVFIVSLTVKRGQINILDLMVFLIALTVSVIPE
                     ALPLVTTISLSHGARQLAKKKVIVKRLSAVEDLGGIEILCSDKTGTLTENNLTIHEIY
                     SKKPDATLLAAAQASAFNEQMSEPFDIAIWQKISDAEQAFIKQTIKLTEEPFNPTIGR
                     NIVLVEDPEGHHLITRGAPEQIIACSQNLTNKERQKIIDWVAKEGHEGRRTLAIASQP
                     LSGFTIKEIKNLALVNNFNFLGVISFVDPIKTSTKAAVKKAKSLGVSIKIITGDSLEV
                     AIAVAKEIGLIKRSNQAMTGTAWQQLSPAQKVKALNNKVVFARVNPEQKYEIVNTLRH
                     HCNVGFLGEGINDAPALKIAGVSLVVNSAADIAREAADIILVNKNLNVILDGIEYGRR
                     VFANTTKYIKATLASNFGNFFAVATASLLVPFLPMLPVQILLLNLLSDMPMVMIA"
     assembly_gap    1344..1443
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1606..1761)
                     /locus_tag="PK960_02215"
     CDS             complement(1606..1761)
                     /locus_tag="PK960_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08013.1"
                     /translation="MRKKKTQVNLLSLKDYLGDREWEQLSQEEQKEICQKILEALFPE
                     SQDSKKN"
     gene            complement(1901..2980)
                     /locus_tag="PK960_02220"
     CDS             complement(1901..2980)
                     /locus_tag="PK960_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08014.1"
                     /translation="MWQTIKEKWRAYCKRFYTGRPWLVVLDVGLIFFIILFFCLWRLL
                     VYYEKLPIEGDLNLPAHKVAGYKTMPLEWQTVFSSKVIDSSLKELTAEISYKNLASEP
                     LIINYQCAEAKTHKSLAFRLEDDEATTSVNILEKQLMPQESGTSSLVLFLSDDLLIKG
                     QKLNIDCQLGARLGQQSWPAIHQQFSFKIAGEVKASAGGYFYTPEGDQVGIGPLPLTV
                     GLPTSYLITWTLENQGGDLSDIQFSAQLAKDVTWQGEAGLTGGTLFYDASNHKVNWRI
                     AEWPADVTKKQANFYISLTPTKDMVGKVPLLLYDGQWRAQDSWSEKEWGGNLPDLSTN
                     LEYDPHSAGQGEVVDILGVDNVIIE"
     gene            complement(3010..>3192)
                     /locus_tag="PK960_02225"
     CDS             complement(3010..>3192)
                     /locus_tag="PK960_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08015.1"
                     /translation="GEIVAVASAGGGPQVRIFNRYGKLLGQFQAFREEAKTGYEVMIN
                     DLDGDGRGEILVGKIN"
     assembly_gap    3193..3292
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3293..4863)
                     /locus_tag="PK960_02230"
     CDS             complement(<3293..4863)
                     /locus_tag="PK960_02230"
                     /inference="COORDINATES: protein motif:HMM:NF026235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HPD08016.1"
                     /translation="MKKQSLVIVILILFGILIATGVKASPQLKDDYPRLANYYLKYFG
                     DVSVAELNNLKKWDLLILPNDYQWAFPEFFNEYKQAKPQGLILAYTYPAMVMGSNLNS
                     LFRTVEAENLWLRDANGLKVEMWPGLYAVNINDLRWQSANEQFLSQELDWHKWDGLMF
                     DSVEDSLARFSHNGLDINGDGRADSLDTVDSLWQQGLVRLFTTTRDSIGNGKLMIING
                     SSLPGYQGKINGRMFETFPAPWEGDGSWTASMEQYLQKLPGQNYPPTLYVINANTNNT
                     GKKDDYQKMRFGLASTLMGDGYFSFDYGDQAHEQTWWYDEYSVNLGQPVSAPYNLLAP
                     QNNKIQPGLWRRDFEFGVAVVNSTNKPQIYTLDKEELEKIRGSQDPLVNNGQIVNYLA
                     LKPNDGLILLKRNLNIVGQTFYNGGFVRVFNQRGQSERNGFFAYQPNLAGGDQVILAD
                     IDGNDTLEQIVNRHSEVIVTRRGQIIHKWYPFGKLYLSRPSLALYPKGKQPPDIIVGA
                     GENTKPVVRFYNYQGR"
     gene            complement(4860..6074)
                     /locus_tag="PK960_02235"
     CDS             complement(4860..6074)
                     /locus_tag="PK960_02235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD08017.1"
                     /translation="MRVIQVNKFNYLRGGAEKYFLELSSKLETAGYEVAKFCMADPRN
                     LEDPWSKYWPSNINLETPSLGDRLRAPGRVIYSKRAKHNLMAMIKVFKPDLIHVHNIY
                     HQLSPSILDAARLANVPVIMTLHDYKIICPNYLLLNHGVICEKCLDGNFWHCFRERCF
                     KDSWSQSLLASLEATYHQHHKTYERGVKLFIAPSHFMADKCEQAGWPKDKLRIIINPV
                     SLLSTVKRQPQNYFLYVGRLSKEKGVDILLKAIAQTNYPLKIAGSGPEENHWRTLAQE
                     LGLENQVEFLGQKTPEEVSQLLAGAIAVVIPSIWYENMPFSLLEALAHGCPVVASNIG
                     GLPEIIKIGYNGWLCQPGEVKSLVQALNLAQEADPILMGQQARESVRGYGWDYHLKAI
                     KAIYQEIVGSKV"
     gene            complement(6081..7199)
                     /locus_tag="PK960_02240"
     CDS             complement(6081..7199)
                     /locus_tag="PK960_02240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD08018.1"
                     /translation="MSELSTKKVKIAYIIPTLSGGGAERLVVDLATHLPTSLYTVFIL
                     ILKDTATPAWEEELAQANIAVVRFHQKFKLDIFCIWRLTHWLNKNHFDILHTHLFGGD
                     FYGRIAGHLAGVPHIVGTEHNINLAEGYLRRWLKRLTARWAEKTIAVSKAVLDYVERY
                     EGIRPDQALVIYNGVALEKFKPSYFLTNANWPHNPKKIIVGGLGRLEHQKNWSCLLRA
                     AVYLPHNVIIRIAGVGREKKGLEQLICALGLEKRVELVNWQASTDFLKSIDIFVLPSW
                     WEGLPVALLEAGAVGLPVIVSDIPSNREIISDTNLGLWFDPNHPEELAEKIQSVLADP
                     KLALKMAVNLHKLVEMQFSLDFMIERYCEVYADLLNKQ"
     gene            complement(7171..8334)
                     /locus_tag="PK960_02245"
     CDS             complement(7171..8334)
                     /locus_tag="PK960_02245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD08019.1"
                     /translation="MKQLKRDKQRIKIVLITPRLAIGGAERLLIDIAKKLNRERYEVY
                     WCALEPTNDAASSWEQEITAAGIPFRVLASDKRSLYKLGRLWRLVKIGFRLWRYLREI
                     RPDIMQTQLFADLYRPIGRLARVPIIIGVEHNLDYDEPHLFQIIKKLCHASADGVIAV
                     SEAVRQDIIKRYSYTPNKVIVIYNGIDETKFYQPLSVSKMHDARSPWQIGGAGRLVPQ
                     KGFDILIKALAKLPAEIKWRCQIAGAGPLQDELASLIKNLNLEHKVSLIGVHSDLRSF
                     FSRLDIFVIPSCWEGLGLVALEAGAAGVPVIASATGGLQEIIQHRYSGFLVDNLQPET
                     LAQALEYIFIGDGAQELSIWAKNWQKEVMSRFTITQTACHYEEYYERIIHKKS"
     gene            complement(8312..9604)
                     /locus_tag="PK960_02250"
     CDS             complement(8312..9604)
                     /locus_tag="PK960_02250"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPD08020.1"
                     /translation="MKQVVSPTIQTIKMSSDKKPLNFYKIIVNGGLFLTALSLPLLDW
                     RFYISGLEFNISDFLILLTAIVFLIYRLGQRFAGQGVEKWQWPLARPMFCFLLVALLS
                     SLFSPWRGESLKYFIRIILFVYLLYIVLPVNALEAWRDWRWPFYGTLIAGVGSGIVGI
                     MSLFGQDWSNSFFRVQPIAIFGHWWMGSNHNLLAEFLVITNFFLLAWGYWQKDERWRR
                     LTQVLFVFFSLITLLTFSRAAWLVIAVQLVGAWLLLTKPRKRVGWLLGGLLLILLSVP
                     LWVKMGQLQEANFSSTANHILLTEIAWQSLQQRPIIGWGSGQFLTLVDHNLRFRAKYG
                     DPIDSHGIIQKVAAENGSLGLVALVAVAVSLFILWGKAWRKYGRQNLWLGCLILGAGG
                     GLLFQLFNTSYYKGKVWLPVALSLTAIKLLENETAKKR"
BASE COUNT         3024 a         2171 c         1535 g         2749 t
ORIGIN      
        1 actttatttt aaaacttatt ggctatacat tattaattgt ttttattgta agcttaacgg
       61 tcaaaagagg acaaattaat atccttgatt taatggtttt tctaattgct ctaactgtta
      121 gcgtcatccc cgaagcctta cctttagtta ctaccatctc attatcacat ggcgctcgcc
      181 aactagctaa aaagaaagta atagttaaaa gactttcagc tgttgaggat ttagggggca
      241 ttgagattct ctgtagtgat aaaactggca ccctcactga aaataattta actatccatg
      301 aaatttattc taaaaaacct gatgccactt tattggcggc cgctcaagcc tcagctttta
      361 atgaacaaat gtcagaacct tttgacatcg ccatttggca aaaaatatca gacgccgagc
      421 aagcttttat taaacaaact atcaaactaa ccgaagaacc atttaatcca accattggcc
      481 gcaacattgt tttagttgaa gacccagagg gccaccatct tattacccgg ggtgctccag
      541 aacaaattat tgcttgttct cagaatttaa ccaacaaaga acggcaaaaa atcattgact
      601 gggttgctaa agaaggtcac gagggaaggc gcactctggc aattgctagt caaccactat
      661 cagggtttac tattaaagag atcaaaaatt tagccctagt aaacaatttc aattttttag
      721 gagttatctc ctttgtagac cccatcaaaa ctagtacaaa agcggctgtc aaaaaagcca
      781 aatctttagg agtgagtatt aaaattatta ctggtgatag tctagaagtg gccatagccg
      841 tagctaaaga aattggcctc attaaacgtt ctaaccaagc tatgactggc acagcctggc
      901 aacaattatc acccgcccaa aaagtcaaag ctttaaataa taaagtagta tttgcccgag
      961 ttaatccaga gcaaaaatat gaaattgtta atacattaag acatcactgt aatgtagggt
     1021 ttttggggga gggtattaac gatgctccgg ccctcaaaat tgctggagtt tctttagtag
     1081 tgaatagtgc ggcagatatt gcccgggaag ctgcggatat tattcttgtt aataaaaatt
     1141 taaatgttat cttagatggc attgaatatg gtcgccgtgt ctttgccaat accacaaaat
     1201 atattaaagc tactctagct tctaattttg gcaatttctt tgcggtggcc actgcttctt
     1261 tactggtacc gtttttacca atgttgccag tgcaaattct gttacttaat ctattatctg
     1321 acatgccaat ggtaatgata gctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnngctaata atagctatcg tgcttcttta ttttgccttt tcagaggctg gtaaattgct
     1501 atattaccat aaatataaaa ctaattacca cctatcagat taaactttcc cataaaccca
     1561 aatcatagct tcttattaac tttccaagct tccagatact ggccattaat tcttttttga
     1621 atcttgagat tctgggaaca aagcctctaa tatcttctgg cagatttcct tttgctcttc
     1681 ctgacttaac tgctcccact ccctgtcccc tagataatcc ttcagagata ataaattgac
     1741 ttgcgtcttt tttttgcgca tatattttat tatatacttt tagtaaatta ttcccaagga
     1801 cttgtatctt tgtatacttc gcagttttgt gtttcttggc ctttgctggt attgacccta
     1861 ctggcttaat ttttaataaa ctgctataga aataacaaca ttattctatt attacattat
     1921 ctacacctaa tatatctact acctcacctt gtccagctga atgtggatca tattctaaat
     1981 tagtagatag gtcaggtaaa tttcctcccc attctttttc tgaccaacta tcttgagccc
     2041 gccactggcc atcatacagc aaaaggggta ccttgccaac catatcttta gtaggagtaa
     2101 gggagatata aaaattagct tgttttttag ttacatcagc cggccattca gcgatacgcc
     2161 aattaacttt gtgattagaa gcatcataaa ataaagtgcc accagttaag ccagcttcgc
     2221 cctgccaagt tacatctttg gctagctgag ccgaaaactg aatatcactc aaatcaccac
     2281 cttgattctc tagagtccaa gttattaaat agctggttgg caatcccacg gtcagcggca
     2341 aaggaccaat acctacttga tccccttcgg gcgtataaaa ataaccacca gccgaagctt
     2401 taacctcacc agctatttta aaagaaaatt gctgatgtat tgccggccaa gattgttgcc
     2461 ctaatcgggc tcctaactga cagtctatat ttaatttttg tcctttaatg agtaaatcat
     2521 ctgacaagaa taaaaccaaa ctagaagtgc ctgattcctg gggcattaat tgcttttcta
     2581 atatatttac actagttgtt gcttcatcat cttctaatct aaatgccaaa gatttatgag
     2641 tcttagcttc agcacattga taattaataa taagtggctc actggctaaa tttttataac
     2701 taatctcggc tgtaagctct ttaagggaac tatcaataac tttggaacta aaaaccgttt
     2761 gccattctag gggcattgtt ttatagcctg ccactttatg agctggtaaa ttaagatcgc
     2821 cctctatggg gagcttctca taataaacta aaagtcgcca taaacaaaaa aataaaataa
     2881 taaaaaaaat aagacccacg tctaagacta ccagccaagg gcgtccggta taaaaacgct
     2941 tacagtaggc tctccatttt tctttaatag tttgccacat atatttatta taccacttta
     3001 ttataacatt taattaattt tacccactaa aatttctccc cgaccatcac catctaaatc
     3061 attaatcatt acctcataac cagttttagc ttcttcccta aaagcctgaa actgacccaa
     3121 gagtttacca taacggttaa aaattcttac ctggggtcca ccgccagcac tagccactgc
     3181 tacaatctcc ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgcccctg
     3301 ataattataa aaacgaacca ccggtttagt gttttcacct gctccgacaa taatgtcagg
     3361 tggctgcttg cccttgggat aaagggctaa gctggggcgt gataaatata gcttaccaaa
     3421 aggataccat ttatggataa tctggccacg tcttgtaact atcacttcac tatgtcgatt
     3481 aactatctgt tccaaggtat cattgccatc aatgtccgct aaaataacct ggtcaccgcc
     3541 agctaaattg ggttgataag caaaaaatcc attacgttcg ctttggcctc gctggttaaa
     3601 gacacgcaca aaacccccat tataaaaagt ctgacccaca atatttaaat ttctttttaa
     3661 tagaatcagc ccatcattag gtttaagggc taaataatta acaatttgcc cattatttac
     3721 taaagggtct tgactgccac ggattttttc taactcctct ttatctaaag tataaatttg
     3781 cggtttatta gtagaattca ctactgccac tccaaattca aaatcacgac gccataatcc
     3841 gggctgaatt ttattattct ggggcgctaa taaattatag ggagcggaaa ctggttgccc
     3901 taaattaact gagtattcat cataccacca tgtttgttcg tgggcttgat ctccataatc
     3961 aaaagaaaaa taaccatcac ccataagagt gctggcaaga ccaaagcgca ttttttgata
     4021 gtcatccttc tttccagtat tattagtatt ggcattaata acatatagcg tagggggata
     4081 attttgccct ggcaatttct gaagatattg ctccatcgag gcagtccaac tgccatcacc
     4141 ctcccaagga gcgggaaaag tttcaaacat ccgaccatta atcttacctt gataacctgg
     4201 cagggagcta ccgttgataa tcattaattt accgttacca atagagtcac gggttgtggt
     4261 aaacaatcta accagccctt gctgccataa gctatcaaca gtatctaaag aatccgcccg
     4321 accatctcca ttgatatcta aaccattatg actaaaacgg gccagactat cttcaacaga
     4381 atcaaacatc aatccgtccc atttgtgcca atctaattct tgacttaaaa attgctcatt
     4441 ggctgattgc cacctaagat cattaatgtt gactgcatat agtccgggcc acatctccac
     4501 ttttaaacca ttagcgtctc gcaaccataa attctcagct tctactgtcc taaataaact
     4561 atttaaattg ctacccataa ccatcgccgg ataggtataa gccaaaatta atccttgagg
     4621 tttagcttgt ttatattcat taaaaaattc aggaaaggcc cattgataat cattgggtaa
     4681 aataagtaag tcccattttt ttaaattgtt aagctcagcc actgaaacat caccaaaata
     4741 tttaagataa taattagcta gtcgcggata atcatcttta agctgagggc tagctttcac
     4801 ccccgtagct ataaggatgc caaacaatat caatattaca atgactaaag attgtttttt
     4861 catactttgc ttcctacaat ttcctgataa atagccttaa tggccttaag atgatagtcc
     4921 caaccatacc ccctgactga ctcccgtgct tgctgaccca taagaattgg gtcggcttcc
     4981 tgagccaaat taagcgcttg aactaaactc ttaacttcgc ccggctgaca cagccagcca
     5041 ttgtaaccaa tcttaataat ttccggtaaa ccgccaatgt tactagcaac taccgggcaa
     5101 ccatgcgcca aagcttctaa taaactaaag ggcatatttt cataccaaat agaaggtata
     5161 accacggcta tggctccggc caataattga gatacttcct cgggagtttt ctgtcctaaa
     5221 aattccacct gattttctag acctaattcc tgagcaaggg tgcgccaatg attttcctca
     5281 ggccctgaac cggcaatttt taagggataa ttagtttggg ctatggcctt aaggagaata
     5341 tctactcctt tttccttaga cagtcggcca acatacaaaa aataattttg cggctgtcgc
     5401 tttactgttg ataataacga caccgggttt attataatcc gcaatttatc tttcggccag
     5461 ccagcttgct cacatttatc ggccataaaa tgactaggcg caataaataa tttaactccc
     5521 cgctcatagg tcttatgatg ctgatgatag gtcgcctcta aactagccaa taaactctga
     5581 ctccacgaat ctttaaaaca tctctcccta aaacaatgcc aaaaattacc gtccagacat
     5641 ttttcgcaaa tcaccccatg attaagtagg agataattag gacaaattat tttataatca
     5701 tgaagcgtca taattaccgg cacattagcc agtcgcgcgg catctaaaat agatggcgaa
     5761 agctgatgat agatattatg aacatgaata agatcaggct tgaacacctt aatcatcgcc
     5821 attaaattat gttttgctct cttactatat attacccgac ctggagctcg cagacggtcc
     5881 cccaagctag gtgtctcaag attaatattg ctaggccaat actttgacca tggatcctct
     5941 aaattacgtg ggtcagccat acaaaactta gccacctcat aaccagcagt ctctaattta
     6001 gacgacaact ctaaaaaata tttttccgcc ccaccccgta aataattaaa tttgtttacc
     6061 tgaatcactc gcataacttc ttattgctta tttaataaat ctgcatagac ttcgcagtaa
     6121 cgctctatca taaagtctaa gctaaactgc atctctacta gtttatgtaa attaacagcc
     6181 atctttaaag ctaatttagg atcagcaagc actgattgaa ttttttccgc taattcttcc
     6241 gggtgattag ggtcaaacca taaaccaagg ttagtatcgc taattatttc ccggttacta
     6301 ggaatatccg agacaatgac tggtaaaccc acagctccag cctctaataa agcaactggc
     6361 aggccctccc accaggaagg caaaacaaaa atgtctatgc tctttaaaaa atcagtgctg
     6421 gcctgccaat tgactaattc cactcgcttt tctaaaccaa gggcacaaat cagctgctcg
     6481 agccccttct tctcgcgacc cacacccgca atacgaatta taacattatg gggtaaataa
     6541 accgccgccc taagaagaca ggaccaattt ttttgatgct ctaatctccc cagtccaccc
     6601 actataattt tttttggatt atgtggccag ttagcattag taagaaaata agagggttta
     6661 aatttctcta atgccactcc attataaatt accaaagctt gatctggcct gatgccctca
     6721 tagcgctcta cataatccag cactgccttg gaaacggcaa tagtcttttc agcccaccta
     6781 gcagttaatc tttttagcca gcgcctaaga taaccttcgg ctaaattaat attatgctct
     6841 gtccctacaa tatggggaac accggccaaa tgtccagcta tccggccata aaaatcacca
     6901 ccaaacaagt gagtgtggag aatatcaaaa tgattcttgt ttaaccagtg agttaaacgc
     6961 cagatacaaa atatatctaa tttaaatttt tggtgaaacc gcactaccgc gatattagct
     7021 tgagctaatt cttcttccca ggctggagta gccgtatctt ttaagataag aataaaaacg
     7081 gtataaagac tagttggtaa atgcgtcgcc aaatcaacaa ctaatctctc cgccccccca
     7141 ccagaaagcg taggtataat ataggcaatc ttaacttttt ttgtggataa ttcgctcata
     7201 atactcttca taatggcaag cagtttgagt aatggtaaaa cgactcatca cttctttttg
     7261 ccaattttta gcccaaatac ttaattcttg agccccatct cctatgaata tatattctaa
     7321 tgcctgagct aacgtctcag gttgaagatt gtctactaaa aaaccgctgt aacggtgctg
     7381 aataatctcc tgtagacccc ctgtagcact agcaataact ggaactccag ctgctcctgc
     7441 ctccaatgcc actaacccta agccctccca gcaagaagga ataacaaaaa tatccaacct
     7501 agaaaaaaat gatcgtaaat ctgaatgcac cccaattaaa cttactttat gctctaagtt
     7561 taggttctta attaaactag caagctcatc ctgcaacggt ccggcgccag caatctgaca
     7621 ccgccactta atctcagctg gcaatttagc tagggcttta attaaaatgt caaacccttt
     7681 ttgcggcacc agacggccag ccccaccaat ctgccacggg cttcgagcat catgcatctt
     7741 acttactgaa agtggctgat aaaatttagt ttcatcaata ccattataaa taacaattac
     7801 tttattgggg gtatagctat aacgtttaat aatatcctga cgaacggctt cagaaacagc
     7861 aataacgcca tcagctgaag catgacataa ttttttaatt atttgaaata aatgcggttc
     7921 atcataatct aaattatgct ctaccccaat aataataggc acccgagcta ggcggcctat
     7981 tggtcgatat agatcagcaa acagctgagt ctgcataatg tctggcctaa tttctcttaa
     8041 ataacgccag agtctaaaac caattttcac taatcgccat aatcgtccta gtttataaag
     8101 cgaacgctta tcactagcta acactctaaa aggtatacca gcggctgtta tttcctgctc
     8161 ccaactacta gctgcgtcat tagttggttc tagggcgcac caatatacct catagcgctc
     8221 acggttaagc tttttagcaa tatcaattaa caatcgctcc gctcctccaa tggctaaacg
     8281 aggcgtgatt aaaactatct ttattcgctg tttatctctt tttagctgtt tcattttcta
     8341 acaatttaat agctgtcagg ctcagcgcta ccggcaacca aacctttcct ttataatatg
     8401 aggtattaaa caattggaat aataaaccac cacccgctcc taaaattaaa cagcccagcc
     8461 ataaattttg acgtccatat ttccgccaag ccttccccca caaaataaag agactcacag
     8521 ctactgctac taaagcaaca aggccaaggc taccattttc tgccgcaact ttctggataa
     8581 taccatgaga gtcaatggga tcgccatatt tagcccgaaa acgcaaatta tggtcaacca
     8641 aagttagaaa ttgcccactc ccccaaccaa taatcggtcg ttgttgaaga ctctgccaag
     8701 caatctcagt taataatata tgattagccg tggagctaaa attagcttcc tgtaattggc
     8761 ccattttaac ccacaagggc acacttaata aaattaataa taaaccgccc aatagccagc
     8821 ctacccgctt ccgtggctta gttaacaata accatgcccc tactaattgg acagctatta
     8881 ctagccacgc tgcccgggaa aaagttagta aagtaatcaa ggaaaaaaag acaaatagca
     8941 cctgagtaag gcgacgccac cgctcatctt tttgccaata gccccaggct aaaagaaaaa
     9001 aattagtaat aaccaaaaac tcagctaaaa gattatgatt agagcccatc caccagtgac
     9061 caaagatagc aatgggctga acacgaaaaa aactattaga ccaatcctgg ccaaataaag
     9121 acataatccc cactatccca ctgcctactc cagcaattag cgtgccatag aaaggccaac
     9181 gccagtcccg ccaagcttcc aacgcattaa ccggtaaaac tatatataaa agatagacaa
     9241 agagaataat acgaataaaa tatttaagcg attcccctct ccaggggcta aaaagcgagc
     9301 taagtaaagc taccagcaaa aaacaaaaca ttggcctagc taacggccac tgccatttct
     9361 ctactccttg cccggcaaat ctctgcccca ggcggtaaat taaaaaaact atggcggtta
     9421 gtaaaattaa aaaatcacta atattaaact ctaaaccgct aatataaaaa cgccaatcta
     9481 gaagcggcaa agataaagct gtcaaaaata gaccgccatt aactattatt ttataaaagt
     9541 ttaatggttt cttgtctgag ctcattttta ttgtttgaat tgttggacta acaacttgtt
     9601 tcatacccta aaaatgccac aaaataataa gaaagtcaaa cttaaataat aaaaaggcag
     9661 ctagttaata gctgccaaa
//
LOCUS       DAOOIS010000018         9665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_6246_length_9665_cov_51.108325, whole genome shotgun sequence.
ACCESSION   DAOOIS010000018 DAOOIS010000000
VERSION     DAOOIS010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6246_length_9665_cov_51.108325"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /gene="rsmH"
                     /locus_tag="PK960_02255"
     CDS             <1..490
                     /gene="rsmH"
                     /locus_tag="PK960_02255"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPD08021.1"
                     /translation="IIRDYGEEPWAGPLAKAIAKYRRRQPITRTKELVEIVEQVLPPS
                     YIARSRHHPATKLFQALRIATNQELENLTSVLNQSLSLLCPHGRIVAVTFHSLEDRIV
                     KHFFLRESKDCLCPPEFPICQCGHHAQLKIIYRHPLRASEAEKAVNPRARSAKLRAAS
                     KL"
     gene            511..861
                     /locus_tag="PK960_02260"
     CDS             511..861
                     /locus_tag="PK960_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08022.1"
                     /translation="MTVSHQELVSPNCLRQKSANTKKWQWRRINYCLWSGIVLLILAY
                     LVVANNLAIQTFALRDYKKQLAKLEQEQADLQTEVAKLNSYSYLQQQVQNLGMVPVSH
                     LSYAVSANDLMAKK"
     gene            866..3079
                     /locus_tag="PK960_02265"
     CDS             866..3079
                     /locus_tag="PK960_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPD08023.1"
                     /translation="MPRPDRLVAQSSRFNIHNQQKKKFNTNLKLVLIFFIIAGLTVVA
                     RLFWLQIINHDYYLAKARDQQEFYAELAPTRGQIFVYDKTANGGKELYPLATNRRLFN
                     LYAVPRDLEIDKINQKREEQTKARERGTADYLLPVIIDPTDLATVLFEAFDQPAIIAE
                     IKQAEQNNLKQQLDEEMSKLPSASSSEEIATAREAILKKQQDLLADKFYQEMAQARYD
                     AALKEKSEAKIADYLKILNKGNDPYEVITKKLEGEQILKLYFKILSRVWGDATLSPTD
                     LSLDGETVIWQPKNEDEKYAITVNGLGQTSREYRYYPEKNIEANILGFVSFANDDDLI
                     GQGKYGLEEAFDDILAGRAGLVKGDVSANRAVILGGRDYRAQTNGSDLILTIDRSIQN
                     FACTKLLEAVAKHQADGGSVVIIDPKTGAVLAMCSAPNFDPNNYSLVKDLRQFNNQST
                     FEAYEPGSIFKAITMAIGIDQGKVTPQTTYNDSGSVMVGHNKINNSDFKGRGVVNMVR
                     VLQDSLNTGAVFVMRQIGLENFRAGVVKFGFGEKTGIELPAESAGSISNLYEKFGQDI
                     NAATASFGQGINVTTLQMAMAFGALANEGKLMRPYLVQEIVNPDGTRITTEPQQVGQA
                     ISPEAARTVSGMLVNVVEDGHGKKAGVEGYYVAGKTGTAQVPRKDGRGYIAGVNIGSF
                     AGYAPVDNPAFVMVVRIDHPRGVVWAESSAAPLFGEIANFILKYKQIPAERPIKN"
     gene            3088..4392
                     /gene="murF"
                     /locus_tag="PK960_02270"
     CDS             3088..4392
                     /gene="murF"
                     /locus_tag="PK960_02270"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPD08024.1"
                     /translation="MFQSLARYILKILSRAVVEKYQPKIIGITGSVGKTTTKEAILLV
                     LSKKYRVRGNIKNYNNEIGLPLTILGSTSPGRSPWGWVKVIIGALGLLIFRDKNYPEF
                     LVLEMGVDHPQDMDYLLSVVQPIIGVVTVIGQAHLEFFGTPDSIAEEKGKLVASLPPS
                     GIAILNVDDRRVAAMASRCKGKVITYGFGPEAQVRATEIIFNYRQASKLSIEALTGIS
                     CELHYHESMVPLSLPLVMTEAALYSALAAVAVGLSEDLGLIEIADILKDFRPLPGRFN
                     LLSGFNDSIIIDDSYNASPTSVLAALEILANLPVDDSAKRIAVLGDMLELGLDSPAEH
                     RRVGQVVAGLPIGHLILVGPLAKLIGEEAQNMGWSGKLDYFNDSSEAALKIQEIISSP
                     ATILVKGSQGARMEKIVKELLANPQEASRYLVRQDGHWLANF"
     gene            4414..4761
                     /locus_tag="PK960_02275"
     CDS             4414..4761
                     /locus_tag="PK960_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08025.1"
                     /translation="MNIIWLISREILAWPFWWYSYGWSFWMKYWGRWLKKQGHSLALL
                     VWIKNIFVPMYGQRDIWSFIISFVVRLVQIIVRLAIWLICVAVTVIAIILWPLAPLMI
                     FYYLYILYYGGRK"
     gene            4745..7477
                     /locus_tag="PK960_02280"
     CDS             4745..7477
                     /locus_tag="PK960_02280"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPD08026.1"
                     /translation="MADENKINGWFVCENCHGLGVDNGRPCRNCKGWGVGWFGANCWF
                     FVDWPTRWQKLKEKLISQKILNILDVLIYLAAISGVIVIVWYIGQATSWFINWSAVGT
                     VDWWIQPVWPLRWLAWSAVFGLLTVYRLIKNSQIARLPRWPDVEVAMPLDWPGVFELP
                     SRNKLNVIDYLPQEIFSSLARATVRAGQNKVDQVSPEGWFGVLLNDSLVKALLFRLNL
                     NGQDIADKLKKYEVKGENHSLTVSLSSEVKIALIQGVASAWSTNDLPLSVDFLAPLIH
                     HSSLVKEIIYDLGAKEEQFAAVANWFHINRHLAKTYKQMRDLAQFKPSSNMDRAYTAK
                     ATPFLNHYAHDLTLAAKNNQLPLCVARQKELSQILDAWSAGRLGIILVGSAGVGKTSL
                     VYGLAQLMVEENVPSFLQDKRLVEVDVPSLIGGVSPEEAQANLERIIVEVNQAGNIIL
                     FFDQVDKIAGLTVGEEQSLDLSDILANALQRGALRCITTASMENYRKYIAERLLGANL
                     AKIDVTEPDDQQLMIMAEHWASYWEKRWGVVFTYAALETAKMVSERYTTEPAQPTRLL
                     GVLEGSARLALRQPGVKLVSEAVVREYASKEFGVPIEQVGREESEMLLNLEEIIHKRL
                     IGQEEAVNMVAASLRRARVELRDTNKPIANFLFLGPTGVGKTELAKTLAEVYFGDEEA
                     MIRLDMSEYQLLGSVNKMIGHDEEAGYLTEAVRQHPFSLILLDEIEKAHSDILNLFLQ
                     VFDDGRLTDAQGRTIDFSNTIIIATSNAGAPYIMEAVQQGVDFNAIRQHIIDNELVQV
                     MRPELINRFDGIVVFRPLTMEDMVAITNLQLQKIGRQLAEKNINFEVTEQGLAYLAKA
                     GYDPKFGARPLRRLLQDTINNVLAEKLLAKELKRRDTVIINEDGQIEIRSAQQL"
     assembly_gap    7599..7698
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7699..7899
                     /locus_tag="PK960_02285"
     CDS             <7699..7899
                     /locus_tag="PK960_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HPD08027.1"
                     /translation="SKLVVETNSGRKEFTIVSFNEADPVNGKISNESPLGQALLNHQA
                     GDKVKVNTPKGYIEYTIVSVTN"
     gene            8004..>9665
                     /locus_tag="PK960_02290"
     CDS             8004..>9665
                     /locus_tag="PK960_02290"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPD08028.1"
                     /translation="MVKINHRPSQTKYIFVVGGVMSGVGKGITAASIGRILINRGYKV
                     SAIKIDPYINVDAGTMNPLEHGEVFVTEDGDETDQDIGNYERFLNSNIYKENYMTTGR
                     VYQAVIERERNLGYGGKCVQVVPHIPLEVRARIEAAVGKTGAEIMIIEIGGTVGEYEN
                     MLFLEAARVMRLENPGSVLFVMVTYLPVPAKIGEMKTKPTQHAVRALNSAGIQPDFII
                     ARSETPIDEPRRKKIAMHCNVHSDDVIAAPDIDSIYDVPGNFEKNHLSAKILKRLGLR
                     PRCNDWGAWQVMAEKVKESREGQRPVKIGIVGKYFTSGDFCLTDSYISVIEAIKHAGV
                     ANNCRPVLGWLNAEDVEKQGVSILENYDGLVIPQGWGKRGYRGKIQAIEYCRTHGVPY
                     LGLCYGMQMAVIEFARNVCGLKGANSEEINPKTKYPVIHIMPGQAELLASKQYGGTIR
                     LGGWPCKLVPGTHLAHIYAQKFGPKVEVVSERHRHRYEFNNDYRKILEEHGLTIAGTS
                     PDGQIVEAIELTDHPFFIGTQFHPEYISRPLDPHPLFVEFVKACLK"
BASE COUNT         2786 a         1555 c         2281 g         2943 t
ORIGIN      
        1 cattatccgt gattatggtg aggaaccctg ggctggacca ttagctaagg ccattgctaa
       61 atatcgtcgt cgtcagccaa ttactcggac caaggagtta gtagaaatag tggaacaggt
      121 actaccacct agttatatag ccagaagtcg tcatcatccc gccacaaagt tgtttcaggc
      181 tttacgtatt gccactaatc aggagctaga aaacttaacc tctgtcctta atcagtccct
      241 aagcttgttg tgcccccatg gccgcattgt tgctgtgact tttcatagct tggaagatcg
      301 gatagttaaa cattttttct taagggaaag taaagattgc ctatgcccgc cagagtttcc
      361 tatctgtcaa tgcggccatc acgcacagtt aaaaataatt tatcgtcatc cgcttagagc
      421 tagcgaggct gaaaaagcgg ttaatccgcg agctcgtagt gctaaattac gcgctgccag
      481 taaactttaa ttaaatatta attcacccct atgacagtca gccaccaaga actagtttct
      541 cctaattgtt taaggcaaaa atcagccaat actaaaaagt ggcaatggcg gcgaataaat
      601 tattgtttat ggtcagggat agttttatta attttagctt atttagtcgt agccaataat
      661 ttggctatcc agacatttgc cctgcgagat tataaaaagc aactagctaa attagaacaa
      721 gaacaagctg atttgcagac cgaggtagct aaacttaatt catatagcta tttgcagcaa
      781 caagtacaaa atttgggtat ggtgccagtg agtcatttat cttacgcagt aagtgccaat
      841 gatttgatgg ctaaaaagta attttatgcc ccggccagac agactagtgg ctcagagcag
      901 ccggtttaat attcataacc aacaaaaaaa gaaattcaat actaatttga aattagtgtt
      961 gatttttttt attattgctg gtcttacggt agtagccaga ttattttggc tacaaattat
     1021 aaatcatgat tattatttag ctaaagcgcg cgatcagcag gaattttatg ctgagcttgc
     1081 cccgacccga ggtcaaattt ttgtttatga caagacagct aacggtggca aggagcttta
     1141 tcctctagct actaatcggc gactttttaa cttatatgca gtaccgcgtg atttggagat
     1201 agataaaatt aatcaaaaga gagaagaaca gactaaagct cgggaaaggg gaacagctga
     1261 ttatttattg ccagtcatta ttgatcccac tgatttagct acggttttgt ttgaagcttt
     1321 tgaccagcca gcaattattg ccgagatcaa acaggctgaa cagaataatt tgaagcaaca
     1381 attagatgaa gaaatgtcta aattgccttc ggcgtccagc agtgaagaga tagccacagc
     1441 tagagaagct atcttaaaaa agcaacagga tttactagca gataaatttt accaagagat
     1501 ggctcaagct cgatatgatg ccgcccttaa agaaaaatca gaggctaaga ttgccgacta
     1561 tttaaaaatt ttaaataaag gcaatgaccc ctatgaagtt attactaaga aattagaagg
     1621 tgagcaaatt ctaaaacttt attttaagat tttaagccga gtatggggtg atgctacttt
     1681 aagtccaact gatttatcat tagacgggga aacggttata tggcaaccaa aaaatgagga
     1741 tgaaaaatat gctattaccg taaatggcct ggggcaaact agtcgagaat atagatatta
     1801 tccagaaaaa aatattgagg ctaatatttt aggctttgtt tcttttgcca atgatgacga
     1861 tttaattggt caaggtaaat atgggttaga agaagctttt gatgatattc ttgctggtcg
     1921 ggctggttta gtgaagggag atgtgagcgc taatcgggcg gttattctgg gtgggcgaga
     1981 ttatagggcg caaactaacg gttcagattt aattttaacc attgatcgtt ctattcagaa
     2041 ttttgcttgt actaaattat tggaagcagt agctaaacat caggctgacg gcggcagtgt
     2101 agtaataatt gatcccaaaa caggggcagt tttagcgatg tgttcggccc ctaattttga
     2161 tcctaataat tattcactag ttaaagattt acggcagttt aataatcagt caacctttga
     2221 agcctatgag ccgggttcaa tttttaaggc tattactatg gcgattggca ttgaccaggg
     2281 caaggtgacg ccccagacaa cctataatga tagcggttca gtaatggttg gccataacaa
     2341 aattaataat tcagatttta agggaagggg agtagtaaat atggttcgcg ttcttcaaga
     2401 ttctttaaat actggggcgg tttttgtgat gcgccaaatt gggttagaaa attttcgggc
     2461 tggggtagta aagtttggtt ttggcgaaaa aacaggtatt gagttgccag ctgaatcggc
     2521 cggcagtatt agtaatttat atgaaaaatt tgggcaagac attaatgcgg ccactgcttc
     2581 atttggccag ggcattaatg taacaaccct acagatggct atggcctttg gagcgctagc
     2641 caatgaaggt aaattgatga ggccttattt agtgcaagag attgttaatc cggatggcac
     2701 aaggattact actgaaccgc aacaggttgg tcaggctatt agtcccgaag cagctagaac
     2761 ggtcagtggc atgttggtta atgtagttga agatggtcat ggcaagaaag ccggcgtgga
     2821 gggttattat gtggctggta aaaccgggac cgctcaagtt cccagaaagg atgggcgagg
     2881 ttatattgcc ggagttaaca ttggctcttt tgctggttac gcccctgtag ataatccagc
     2941 ctttgttatg gtggtgcgta ttgatcatcc gcggggtgtg gtctgggctg aaagttcagc
     3001 cgctccatta tttggtgaaa ttgctaattt tattttaaaa tataaacaga ttccagccga
     3061 acggccaatt aagaattaat tttttttatg ttccaatcat tagcgcgtta tattttaaaa
     3121 atattatctc gggcagtagt agagaaatac cagcccaaga ttattggtat tactggcagt
     3181 gtgggtaaga caactactaa agaggcgatt ttattagttt taagtaaaaa gtatagagta
     3241 agaggtaata taaaaaatta taataatgaa attggtttac cgcttactat tttaggcagc
     3301 acctcacctg gtcgttcgcc ttggggatgg gttaaggtta ttattggggc tttaggatta
     3361 ttaattttta gagataaaaa ttatcccgaa tttttagttt tagagatggg ggtagaccat
     3421 cctcaggata tggattattt attaagtgtt gttcagccaa taattggggt ggtgacagtg
     3481 attggccagg ctcatttaga attttttggc actcctgata gtattgctga agagaagggc
     3541 aaattagtgg ccagcttacc cccatcgggt atagctattt taaatgttga tgatcggcga
     3601 gtagcagcta tggcttcaag gtgtaagggt aaagtaataa cctatggttt tggtcctgag
     3661 gctcaagtta gagcgacaga gataattttt aattatcgtc aagctagcaa gctatctatt
     3721 gaggccttaa caggaataag ttgtgaactt cattatcatg agagtatggt tcccttatct
     3781 ttacccctgg ttatgaccga agctgctctt tattcagcgt tagcagcagt cgccgtggga
     3841 cttagtgagg atttaggact tatagagatt gccgatattc ttaaagattt tcggccattg
     3901 ccaggacgtt ttaatttatt gtccggcttc aatgatagta ttataattga tgattcatac
     3961 aatgcttcac ccacttctgt gcttgcggcc ttagaaattt tagctaattt gccagtagat
     4021 gattcagcta aacggatagc agtactggga gatatgttag agctaggttt agacagtcca
     4081 gcagaacatc ggcgggtagg gcaagttgtt gccggtttgc ctattggtca tttaatattg
     4141 gtggggccat tggctaaatt aattggcgag gaggcccaga atatggggtg gtcggggaaa
     4201 ttagactatt ttaatgactc tagcgaggca gccttaaaaa tccaagaaat aatttcttca
     4261 ccagcaacta ttttagttaa aggttcacag ggggctcgaa tggaaaaaat agttaaagag
     4321 cttttagcaa atcctcaaga ggctagtaga tatttagtac gtcaagatgg tcattggtta
     4381 gctaactttt aattttctgc tataatatat cttatgaata taatttggct tattagtaga
     4441 gaaatattag cttggccttt ctggtggtat tcctatggct ggtctttttg gatgaaatat
     4501 tggggccggt ggttaaaaaa acaaggacat agtctggctt tgttggtgtg gataaagaat
     4561 atttttgtgc caatgtatgg ccagcgagat atttggagtt tcattattag ctttgtggta
     4621 cgattagtgc aaataattgt taggttggcc atttggctta tttgtgttgc tgttactgta
     4681 atagccataa ttctttggcc attagcgccg cttatgattt tttactatct ctatatatta
     4741 tattatggcg gacgaaaata aaataaatgg ttggtttgtt tgtgaaaatt gccatggttt
     4801 gggcgtagac aatggtcggc cgtgtcggaa ctgtaaagga tggggtgtgg ggtggtttgg
     4861 cgctaattgt tggttttttg ttgattggcc aaccaggtgg cagaagttga aagaaaaatt
     4921 aattagtcaa aagatcttaa atattttaga tgtattaatt tatttagcgg ccataagcgg
     4981 agtgatagtt attgtttggt atataggcca agctaccagt tggtttatta attggtcggc
     5041 ggtggggacc gtagattggt ggattcagcc ggtatggcct cttcggtggt tggcatggtc
     5101 agcagtgttt ggccttttaa cggtttatcg tttaataaaa aatagtcaaa tagctagatt
     5161 accccgctgg ccagatgttg aagtggctat gcccctagac tggccaggag tttttgagct
     5221 gccatctcgt aataaattaa acgtgattga ttatttaccc caagaaattt tttctagttt
     5281 agctcgagca acagtacgag ccggtcagaa taaggtagat caggttagtc cagaaggatg
     5341 gtttggtgtt ttgttaaacg attctttagt gaaggcctta ttgtttcgtt taaatttaaa
     5401 tggtcaagat attgctgata agcttaaaaa atatgaagtt aagggcgaga accattctct
     5461 gacagtgagt ttgtcttccg aggttaagat agcccttatc cagggggtcg ctagcgcttg
     5521 gtcaaccaat gatttgcctt tgtcagtgga ttttttagct cccctaattc atcacagttc
     5581 tcttgtaaaa gaaatcattt atgatttagg agccaaagaa gaacaatttg cggcggtagc
     5641 taattggttt catattaatc ggcacctagc taagacttat aaacaaatgc gagatctagc
     5701 ccaatttaag ccctcttcta atatggatcg tgcttatacg gctaaggcca caccattttt
     5761 gaaccactat gctcatgatt tgactttagc ggctaagaat aaccaactgc ctttgtgtgt
     5821 agcacgtcaa aaggaactga gccagatact tgatgcttgg tcggctggaa gattaggaat
     5881 aattttggtt ggttcagccg gagtggggaa aacatctttg gtgtatggac tagctcaatt
     5941 aatggtggaa gaaaatgttc cttctttttt acaagataaa agattggtgg aagtagatgt
     6001 gccttctttg attggtggtg tatcgcccga agaggctcag gcgaacttag agcggattat
     6061 tgtggaagtt aatcaagctg gtaatattat tctcttcttt gaccaagtag acaaaattgc
     6121 tggtcttact gtgggggaag aacagagttt ggatttatca gatattttag ctaatgctct
     6181 ccagcgagga gctttgcggt gtataaccac agccagtatg gagaattatc gtaaatatat
     6241 tgctgagcgt ttgctaggag ctaatttggc taaaattgac gtcaccgagc ctgatgatca
     6301 gcaacttatg attatggcag aacactgggc tagctattgg gagaaacgtt ggggggtggt
     6361 atttacttat gctgccctag agacagctaa aatggttagt gaacgttata ccaccgagcc
     6421 agcccaaccg acacgtttgc tgggtgtttt agaggggtca gctcgtttag ccttaagaca
     6481 gccaggggtt aaattagtga gtgaagcggt ggtgagagaa tatgctagta aagagtttgg
     6541 tgtgcccatt gagcaggttg gccgggaaga aagtgagatg ctacttaatt tagaggaaat
     6601 tattcataag cgtttgattg gtcaagaaga ggcagtaaac atggtagccg ctagtttacg
     6661 gcgggcgcga gttgaattaa gagatacaaa taaaccaatt gctaattttt tatttttagg
     6721 accaactgga gttggtaaaa ctgagctagc taagacttta gctgaagttt attttggcga
     6781 tgaggaagct atgattagat tagatatgag tgaatatcaa ttactaggca gtgttaataa
     6841 gatgattggc cacgatgaag aggctggtta tttaactgaa gcagtccgcc agcatccttt
     6901 tagtctgatc ttattagatg aaattgagaa agctcattca gatattttaa atttattttt
     6961 acaagtcttt gatgatggcc gtttgactga tgctcaaggc agaaccattg attttagtaa
     7021 tactattatt attgccacct ccaatgctgg ggccccatat attatggagg cagtccagca
     7081 gggtgtggat tttaatgcta tccgccagca tattatagat aatgagctgg tacaggtaat
     7141 gcggccagaa ttaattaatc gctttgatgg tattgtggtt tttcggccgc ttactatgga
     7201 agatatggtg gcgattacta atttgcaatt gcaaaagatt ggtcgtcaac tggcagaaaa
     7261 aaacattaat tttgaggtga ctgaacaggg attagcttat ttagctaagg ccggttatga
     7321 tcctaaattt ggggctcggc cattacgtcg tctactgcag gataccatta ataatgtttt
     7381 ggctgagaaa ttattagcta aagaattaaa acgtcgcgat acagtaatta ttaatgaaga
     7441 tggccaaata gaaattagat cagctcagca gttataatta tagaagctca taaaaaaaga
     7501 cccttaagct agactattgt atagataggg atggcgtagg gcttgtcaag tttttttctt
     7561 gtggtataat gggctttata atttttaggc gcgaagatnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnntc taaattagta gtagagacta attcaggccg aaaggagttt
     7741 actattgtta gctttaatga agctgaccca gtaaatggca aaatttctaa tgaatcacct
     7801 ttagggcagg ccttgcttaa tcatcaagcc ggtgataagg ttaaagttaa tactcctaag
     7861 gggtatatag aatacaccat tgttagtgtt actaattagt aaaagttttt attaattttt
     7921 attaacaaaa actacttggc agctatagtc gagtagtttt atttttgata tactgaaagc
     7981 agtttttctt tttaaatgtt tatatggtca aaattaatca tcgcccatct cagactaagt
     8041 atatttttgt agttggggga gttatgtctg gggtaggcaa aggtattact gccgcctcta
     8101 ttggccgtat cctaattaac cgtggctata aagtaagcgc tattaaaatt gatccatata
     8161 ttaacgtcga tgcaggaacg atgaatccac tggagcatgg cgaggtcttt gtgactgaag
     8221 atggtgatga aaccgaccaa gatattggca attatgaacg ttttcttaat agcaatattt
     8281 ataaagaaaa ttacatgacc actggccggg tttaccaggc agtcattgag cgagagcgta
     8341 acctaggtta tggcggtaaa tgtgttcaag tagtgccgca tattcctttg gaagttaggg
     8401 ctagaattga ggcagcagtg ggaaaaactg gagcggaaat tatgattatt gagattggcg
     8461 gcacggtggg tgagtatgag aatatgctct ttttagaagc tgccagggtg atgcgtctgg
     8521 aaaatccggg gagcgtatta tttgttatgg taacttatct gccagtacca gctaagattg
     8581 gagagatgaa aactaaaccg acgcaacatg ctgtgcgggc cctaaactca gccggcattc
     8641 agccggattt tattattgcc cgttcggaga cgcccattga tgagcctcgt cgcaagaaga
     8701 ttgctatgca ttgtaatgtc cacagtgacg atgttattgc agccccggat attgattcta
     8761 tttatgatgt cccgggtaat tttgaaaaaa atcatctatc cgccaagata ttaaaacgtt
     8821 tagggttaag accgcgctgt aatgattggg gtgcttggca agtaatggcg gaaaaggtta
     8881 aggagagtag ggaaggacag cgtcctgtaa aaatagggat tgttggcaaa tactttacct
     8941 cgggtgattt ttgtttaact gattcatata ttagtgtcat tgaggccata aaacatgccg
     9001 gtgtggctaa taattgtcga ccggtattgg gttggttaaa cgctgaggat gtagagaaac
     9061 aaggagtaag tattttagaa aattatgatg gtctagttat ccctcagggt tggggtaaaa
     9121 gaggctacag aggcaaaatt caagctattg aatactgccg tactcacggc gtaccttatc
     9181 ttggtttatg ctatggcatg cagatggcag ttattgaatt tgctcgtaat gtttgcggtc
     9241 ttaagggagc caatagtgag gagattaatc ccaagactaa atatccagtt attcatatta
     9301 tgccaggaca agcggaactg cttgccagta aacagtatgg gggtactatt cgtttaggcg
     9361 gctggccttg caagttagtg ccggggaccc atttagccca tatttatgct cagaaatttg
     9421 gtcctaaggt agaagtagtc agcgaacggc atcgtcatcg ttatgagttt aataacgatt
     9481 accgaaaaat tttagaagag catggcctta ctattgctgg cacctcgcct gatggccaga
     9541 ttgtagaagc cattgagctt actgaccatc ctttttttat tggtacgcaa tttcatccgg
     9601 aatatatttc tcgtccctta gatcctcatc ctttatttgt ggagtttgta aaagcttgct
     9661 taaaa
//
LOCUS       DAOOIS010000019         9456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_6404_length_9456_cov_33.052760, whole genome shotgun sequence.
ACCESSION   DAOOIS010000019 DAOOIS010000000
VERSION     DAOOIS010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6404_length_9456_cov_33.052760"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..309
                     /gene="secE"
                     /locus_tag="PK960_02295"
     CDS             127..309
                     /gene="secE"
                     /locus_tag="PK960_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPD08029.1"
                     /translation="MDKIIAYFKSAWAEIKKVSWPTKKETYNYTLLVIGISLGTAIFL
                     GALDALFTVGLTALIK"
     gene            319..864
                     /gene="nusG"
                     /locus_tag="PK960_02300"
     CDS             319..864
                     /gene="nusG"
                     /locus_tag="PK960_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPD08030.1"
                     /translation="MAKQTLNQGRRWYVLHTYSGYEENVAQNIKQRIESMDMEDKIFN
                     VMIPTEKKIKIVNGKRKVYEEKIFPGYILVEMIVTDDSWYAIRNTPNVTGFVGTGTVP
                     TPIDEAEIKELQKRMGLDEPKFKIDVSVGSPVRIVDGPFKNLEGKVTEIDENRGKVKI
                     LISMFGRETPVELDFLQITKI"
     gene            870..1295
                     /gene="rplK"
                     /locus_tag="PK960_02305"
     CDS             870..1295
                     /gene="rplK"
                     /locus_tag="PK960_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPD08031.1"
                     /translation="MAKKILTQIKLQIKGGQANPAPPVGPALGQHGVNISDFCSKFNE
                     ATRDRMGEVVPVVITVYADRSFDFITKIAPAAELIKQAAQINKGSGQPLKDKVGHITM
                     AQLRDIAEKKLPDLNARDIEAAMNIIAGTARQMGVEIKD"
     gene            1357..2076
                     /gene="rplA"
                     /locus_tag="PK960_02310"
     CDS             1357..2076
                     /gene="rplA"
                     /locus_tag="PK960_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPD08032.1"
                     /translation="MVKKRQKTVKPSKKGKAQLAVRPLVDRAKAYPLTEALELAKKTS
                     TVKFDASLEVHIRLGIDPRKGDQQIRGAVSLPHGSGKTVKVAAFVSPENEAAVKAAGA
                     DIVGGEDLIDEIRRTEKTDFQVAVAEPAMMKNLAVIAKILGTRGLMPSPKNETVTTDP
                     AKAVAELKKGKVSFKNDDTANIHVAIGKVSFTTEDLVDNFQALMETIKKSKPATAKGT
                     YIKGITVCSTMGPGIKVASTL"
     gene            complement(2124..2435)
                     /locus_tag="PK960_02315"
     CDS             complement(2124..2435)
                     /locus_tag="PK960_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPD08033.1"
                     /translation="MKHKAIRRQVNKYLRQPVATLDLHGCSRREAEEELTHFIHYWLE
                     QGGGVKLLIITGQGWHSPDGQGIIKPFTINWLKEHGYRYRFAKPQDGGSGALEVDLPI
                     N"
     gene            2505..3035
                     /locus_tag="PK960_02320"
     CDS             2505..3035
                     /locus_tag="PK960_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HPD08034.1"
                     /translation="MPTRPSKMEYYMRIAGEVAGRSTCYRAKHGAIIVKDDQIISTGY
                     LGAPRKTSDCWERGQCLREVMGIPHGQRYELCRSVHAEQNAIINAARAGVSLLGSVMY
                     LYSTVIRDGQEQPQDAYPCFICKKMIINAGLERLVSRQADGHLKVYDISEWVNDWQEH
                     DMLDDTEQYGLAINSE"
     gene            3060..3620
                     /locus_tag="PK960_02325"
     CDS             3060..3620
                     /locus_tag="PK960_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD08035.1"
                     /translation="MDDKLIIGLTGQLAAGKTTVARYLATKHQGKVYGFSGPLRDIAD
                     RLTLPQTRENLANLSSILRRQFGQDIILRAIKRDVATDPCHFIILEGMRRYGDFLSFK
                     DLPNFHLVAVVAEQRLRYERMVKRHQNPDDATKTWEQFQVDDQAEAEQDIPKAIAEAE
                     FIIDNNGDLESLYQQAENIYQKLTSH"
     gene            3622..4206
                     /locus_tag="PK960_02330"
     CDS             3622..4206
                     /locus_tag="PK960_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08036.1"
                     /translation="MSGKLIVLYGINNIGKTTQAQLLVNRLQAAGYLASYLKYPLYEL
                     RPSGPLLNDYLRAGNPWKLSAREAQIIYALNRFQYQEELERELASGQWIVAEDYVGTG
                     LAWGWGAGVDLDFLEAINQGLHQPDLALWLDGERFSSGIEANHKHEGDEELMTKVCAC
                     HRELAKRYGWLRFRANQSRKTLNDELWQAVKTLL"
     gene            4212..5702
                     /gene="gatB"
                     /locus_tag="PK960_02335"
     CDS             4212..5702
                     /gene="gatB"
                     /locus_tag="PK960_02335"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPD08037.1"
                     /translation="MKDLVPIIGLEIHIELKTQSKMFCRCDNNAVGKEPNTVVCPICL
                     GHPGTLPYLNKEAVHYGLLLAQALNFQIDQLSFFARKNYFYPDLPKGYQITQADTPLG
                     SQGYLTIDDHEIAIERLHLEEDTGKLIHDSQSNCSLVDYNRAGTPLIELVTSPVITSA
                     TEARRFCQELQKILRYLNISNANMEQGEMRSEANISLQEKDSFDFVGPYLKVKPGHHL
                     NNKVEVKNLNSFKALERAINYEIERQGQVLASGGAVVAETRGWNETKGITVSQRYKET
                     VADYRYFGEPDLPPLYIDNTWLLEIKQELDKIELPAVKAKRWQKDYGWPADQADLLST
                     YPSSAMYSDELLTTICQIAPGNYKEIARLAGNWLTSEVLKYCSLKDLLLNPITPRHLA
                     DLSIALANKTITSTNGQVVLQTIVKNTKVSVEEIIKQLDLQQVESSDQISKLVQKVID
                     QYPDQAAAYRAGKLALLQFFIGQVMHQSNGKANPKVVREILEKKLQ"
     gene            complement(5714..8797)
                     /locus_tag="PK960_02340"
     CDS             complement(5714..8797)
                     /locus_tag="PK960_02340"
                     /inference="COORDINATES: protein motif:HMM:NF027795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5057 domain-containing protein"
                     /protein_id="HPD08038.1"
                     /translation="MQQLGINNKKFIIGITILIGFSILISRLPNTQAASNSGSPNQSS
                     STIEAGARPSLNPDAIAVRIFDNNNYLSPYDWYKINVGAKRSLQSLIVDGYDAVRDDR
                     TVYVAASNIVPCASGGKECLQPIIAIISFNQNVSNETLDIFGQILNNWHFNQNINSYE
                     GTCWSKPDTPSGINCLTNKDCPSGQYCNSLKSKIIRDTRRLIDLTFIKDKLEQYRTNY
                     QHYPNLEAGSYLINKTLSVWPSWQKTLAVKLNTALPIDPINYLGSCPGYNPNTCWNEQ
                     QRSFATSWPYLPTGSRSYQYEYINSTNYRLCANFESNYQNLNTLNLNCAGTSSNSGPI
                     INCPTLRGAAYQAFTGYAAISDSEGDTIRPNISFTPLENWQNLSMQLVDNRRHIKISA
                     TSAGPTGRYTTTITAQDSLNNIGTGTCTIIIGGGLCGNNAINTGEDCDGEQGIAHSAI
                     ESSPYKQYACNEYCRFTGGWCGDNIVQNGDNYTLNLEQCDGSLGIAYRTSDSNINRQY
                     GCLHCQFTGGYCGDHIVQTSTNSLINEECDHSETLMPQETYGGSQQYYCGQPGTINAC
                     RFAGGYCGNGIVESNYEQCEPASYSTPTPADSSSNHQYLCGTNCATYDGYCGDGTVNA
                     NHNEICDPGSYITPKPASSSPTNQYVCGSDCVTQGGYCGDYTVQYEESCEPIVYITPT
                     PANSSLTHQYRCTNCVTHGGYCGDGTVDATYGEICDPGSYGTSTPASSTPTHQYVCGS
                     DCVTHGGYCGDGTVNTAYGETCDPNALSSWNPNTEFCDSVNCQKGTRLRILQIIPDGT
                     NLNLAKLFASSTYQDNIITQTATVTTTTLAHFNTNSTTYIPTTNPTQFYNVLVFGFKD
                     CNNSQDLNTTSASTTKKFIQQGGGVIFGHDTIWQHGNICGGTHSHFNSLAPYAGINVA
                     SYTNDTTFSSVKKYPTSTSSELMFTTPYSIPSTFSVSPTHNSGAVPSSTLCSATNPNL
                     LRVWYVKNTASATPANFWAITCGRVEHILLGHTATSTSMVENRALINMLYYVATH"
     gene            complement(8893..>9456)
                     /locus_tag="PK960_02345"
     CDS             complement(8893..>9456)
                     /locus_tag="PK960_02345"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HPD08039.1"
                     /translation="LPVRRGRKGEKFVANELYKLLDSNYYRILNNLLLPSTGNLNTTQ
                     IDHVIVSNFGIFCLETKAYAGWIFGNIKQKYWTQVIFHNKERFYNPFYQNYAHIKALE
                     KIIGQSQLKSPIISLVIFTDADKLKISGTDAAGFTFEIIRKIKNYSIPIYTDTERDEI
                     YNLLCQANITDINLQKLHNKNVQRLKY"
BASE COUNT         2928 a         1644 c         1983 g         2901 t
ORIGIN      
        1 acacatggtt ctcagccatg taactggggt tcaactcccc atggtggtac tttcttgaca
       61 atttttatta tcttttctac aataggacta tcttagcccg tcagggctct ttttagaatt
      121 aagattatgg ataaaattat tgcctatttt aaatcagcgt gggcggaaat taaaaaagtt
      181 agttggccaa ccaagaaaga gacctataat tacactctat tagttattgg tattagctta
      241 ggcaccgcta tttttttggg tgccctagat gctttattta cggtgggtct tacggccctt
      301 attaagtaat aattttttat ggctaagcaa actttaaatc aaggtcggcg atggtatgtc
      361 ctccacactt attcgggcta tgaagaaaat gtagctcaga atattaaaca acggattgaa
      421 tccatggata tggaagataa aatttttaat gttatgattc ctacagaaaa gaaaataaag
      481 attgttaatg gcaaacgtaa ggtttatgaa gaaaaaattt tccctggtta tattttagta
      541 gaaatgattg tcactgatga ctcctggtat gctatccgta atactccaaa tgttactggt
      601 tttgtgggaa ctggcactgt gcccaccccg attgatgaag cagagataaa agaacttcag
      661 aagcggatgg gattagatga gcctaaattt aagatagatg tttcagttgg gtcgccggtt
      721 agaattgttg atggtccttt taagaattta gagggcaagg tgacggaaat tgatgaaaat
      781 cggggcaagg ttaaaatatt aatttcaatg ttcggtcgag aaacaccagt ggaattagac
      841 tttttgcaaa taactaagat ttagattata tggctaaaaa aattctgaca cagataaaat
      901 tacaaattaa agggggacaa gctaatccgg ctccaccagt tggtccagct ttaggacagc
      961 acggggtaaa tattagtgat ttttgtagca aatttaatga ggccacccgt gaccgaatgg
     1021 gtgaagtagt cccagtagtt attactgttt atgccgatcg tagttttgat tttattacta
     1081 aaatagcccc agcggctgaa ttaattaaac aagcagcgca aattaataaa ggttcgggtc
     1141 aaccccttaa agataaagta ggccatatta ccatggccca attgcgtgat attgctgaaa
     1201 agaaattacc ggatttaaat gctcgagata ttgaagcagc tatgaacatt attgctggta
     1261 cagcccgaca gatgggtgta gaaattaaag attaaaaatt taataataaa aatcgtggga
     1321 gctgttttta gtgacagcgt tctacaccac aattatatgg ttaaaaaaag acaaaaaact
     1381 gtaaagcctt ctaaaaaagg taaagctcaa ctagcggtac gccccttagt agatcgagcc
     1441 aaggcatatc ctttaactga agctttagaa ttggctaaaa aaactagcac cgttaaattt
     1501 gatgcttctt tagaggttca tattcgttta ggtattgatc cccgcaaggg cgaccagcaa
     1561 attcgcgggg ctgttagttt accccatggg agcggtaaga cggttaaggt ggctgctttt
     1621 gttagtccag aaaatgaagc cgcggttaaa gcggctggtg ccgatattgt gggtggtgaa
     1681 gatctaattg atgaaattcg tcggacagaa aaaacggatt ttcaggtagc tgtagctgaa
     1741 ccagcgatga tgaaaaattt agctgttatt gctaagattt tagggactcg aggattaatg
     1801 ccctcaccta aaaatgaaac agtaactacc gatccagcta aggccgtagc tgaattaaag
     1861 aaaggtaaag ttagctttaa gaatgatgat acagccaata tccatgtggc tattggcaaa
     1921 gtgtcattta ccaccgagga tttagtagac aattttcagg ctctaatgga aactattaaa
     1981 aaaagtaagc cagctactgc taaaggtact tatattaaag gcattactgt ctgttcgacc
     2041 atgggtccag ggattaaggt agcttcaaca ttataatctt tagagtgcat aaataacagc
     2101 caatttgtag tggctgttat tttttaattg attggtaaat ctacttccag agcaccagaa
     2161 ccgccatctt gcggtttagc gaagcgatag cggtaaccat gttctttaag ccaattaata
     2221 gtaaaaggtt taataatacc ttgaccatca ggagaatgcc agccctggcc ggtaataatt
     2281 aataatttta ctcccccgcc ctgttctagc caataatgaa taaaatgggt taattcttct
     2341 tcggcttcgc gacggctaca gccatgtaaa tctaaggtag ctactggctg gcgaaggtat
     2401 ttattgactt ggcgacgaat agctttgtgt ttcataataa atgattatac caactttttt
     2461 agcttgcgca aagcttaaat aatttgtaca atgataaaaa tattatgcct accagaccct
     2521 ctaaaatgga atattatatg agaattgccg gcgaagtagc tggccgttca acctgctatc
     2581 gggctaaaca tggggctatt attgttaaag atgaccaaat tatttcaact ggttacttag
     2641 gggccccacg taagactagt gattgctggg agcgtggtca gtgcctacgt gaagtaatgg
     2701 gtatacctca tggtcagcgg tatgaacttt gtcgtagtgt ccatgccgaa caaaatgcta
     2761 ttattaatgc cgctcgggct ggggttagct tgctggggtc agttatgtat ctttattcaa
     2821 cagttattcg tgatggccag gaacagccac aggatgctta cccttgtttt atctgtaaaa
     2881 aaatgattat taatgctggt ttagaaagat tagttagccg gcaggccgat ggtcatctta
     2941 aggtatatga tattagtgaa tgggttaatg attggcagga acacgatatg ctagatgata
     3001 ctgaacaata tggtttagct attaacagtg aataaggctt tatttagtca aaaatcatta
     3061 tggatgataa gttaattatt ggtcttactg gtcaattagc agccggtaaa actacagtag
     3121 ctagatattt agcaactaag caccaaggta aagtttatgg cttttctgga ccgcttaggg
     3181 acattgctga ccgcttgacc ttgccgcaaa ctagagaaaa tttagctaat ttgtcgtcta
     3241 ttttacggcg tcaatttggc caagatatta ttttaagagc aattaaacgt gatgtggcta
     3301 ctgatccttg ccattttata attttagaag gaatgcgtcg ttatggtgat tttttatctt
     3361 ttaaggactt gcctaatttc catttagtgg cagtggtggc tgagcaacgt ttgcggtatg
     3421 agagaatggt taaacgtcat caaaacccgg acgatgctac taaaacttgg gagcaattcc
     3481 aagtggacga ccaagctgaa gctgagcagg atatacccaa agctatagct gaagctgagt
     3541 ttattataga taataatggt gatttggagt cactttatca acaggcagaa aacatttacc
     3601 aaaaattaac aagtcattaa tatgtctggt aagcttattg ttttatatgg cattaataat
     3661 ataggcaaaa ctacgcaggc tcaattatta gtgaaccgtt tacaagcggc tggttatttg
     3721 gccagttatt taaaatatcc gctttacgag cttagaccca gcggtcccct gcttaatgat
     3781 tatctgcgag ctggcaatcc atggaaatta tcagcccgag aagctcaaat tatttatgct
     3841 cttaaccgtt ttcaatatca agaagagcta gaaagagaac ttgccagtgg tcaatggatt
     3901 gtggctgaag attatgtcgg gactggtctg gcttggggtt ggggtgctgg tgtggaccta
     3961 gattttttag aagccattaa tcagggattg catcagccgg atttggcctt gtggctcgat
     4021 ggtgaaagat tttctagtgg cattgaagcc aatcataagc acgagggaga cgaggaatta
     4081 atgaccaagg tatgtgcttg ccatcgtgaa ttagctaaac gctacggctg gcttaggttt
     4141 agggctaatc aaagtagaaa gactctcaat gatgagcttt ggcaggcagt taaaacttta
     4201 ttataatcat tatgaaagat ttggtaccta ttattggcct agaaattcat attgagctta
     4261 agactcaaag taaaatgttt tgccgttgtg ataataatgc tgttggtaaa gagcctaata
     4321 ctgttgtctg ccccatttgt ttagggcatc ctggtacttt accttatctt aataaagagg
     4381 ctgtccatta tggtcttcta ttggctcaag ccttaaattt tcaaattgat caattatcct
     4441 tttttgcccg caaaaattat ttttatccgg accttcccaa gggttaccaa ataactcaag
     4501 cggatacccc cctagggtct cagggctatc ttactattga tgaccatgag attgctattg
     4561 aaagattaca cttagaagaa gatacgggta aattaattca tgattctcaa tctaactgca
     4621 gtttagttga ttataatcgg gccggtaccc ccctaattga acttgttact tctcctgtta
     4681 ttacctcggc gacagaagca cgtcgttttt gccaagaatt acaaaaaatt ttacgctatc
     4741 ttaatatctc taatgctaat atggaacaag gtgagatgcg ctcagaggct aatattagtt
     4801 tacaagaaaa agattctttt gattttgttg gtccttatct taaagtaaag ccaggccatc
     4861 atttaaataa taaagtagaa gtaaagaatc ttaattcttt taaggcttta gaacgagcca
     4921 tcaattatga gattgaacga caggggcaag ttttagctag tggaggtgcg gtagtcgctg
     4981 aaacacgtgg ctggaacgag acaaaaggta ttactgtatc tcagcgctat aaagaaactg
     5041 tggctgacta ccgctatttc ggtgaacctg acttaccccc tctctatatt gataatactt
     5101 ggttattaga aataaaacag gagctagaca aaatagaatt acccgcagta aaggccaagc
     5161 gctggcaaaa agactatggc tggccagcag atcaagctga cctactatct acatatccta
     5221 gctcagcaat gtatagtgat gaattattaa ctaccatttg tcaaattgct ccaggaaatt
     5281 ataaggaaat agcccgttta gctggtaatt ggttaactag cgaggtctta aaatattgtt
     5341 cactcaagga tttattatta aaccccatta ctccccgtca tttagctgat ctaagtatag
     5401 ctttagctaa taaaaccatt acctcgacta atggacaagt agttttacaa acaatagtaa
     5461 aaaataccaa agtatctgta gaagagataa ttaaacagtt agacttacag caagtagaaa
     5521 gtagtgacca aattagtaaa ttagtgcaaa aagttataga tcaatatccc gatcaggcag
     5581 cagcttaccg ggctggtaaa ctagccttat tacaattttt tattggccaa gtaatgcacc
     5641 agagtaatgg taaggctaac cccaaagtag tacgagaaat actagaaaaa aagctacaat
     5701 aaaccccggc tatttagtga gtggctacat aataaagcat attaattaaa gccctatttt
     5761 ccaccatgga agtcgaggtg gcagtatgtc ccagcaaaat atgttccact cggccacagg
     5821 ttatagccca gaagtttgca ggagtggcag atgcagtatt cttcacatac caaactctta
     5881 ataggttagg attagttgca gaacagagag ttgaacttgg gacagctcca ctattatgag
     5941 ttgggcttac actaaaagta gatggtatag aataaggagt agtaaacatt aattcactgg
     6001 aggttgaagt gggatatttt ttgacagaac taaaggtagt gtcattagta taactagcga
     6061 cattaatgcc ggcataaggt gctagactgt taaaatgaga atgagtgcca ccgcatatat
     6121 taccatgctg ccagattgta tcatgaccaa agattacccc accaccctgt tgaatgaatt
     6181 tcttggtcgt gctagcgctg gtagtattta aatcttggct attattgcaa tctttaaacc
     6241 caaaaactag gacattatag aattgggttg gattagtcgt tgggatatag gtggtagaat
     6301 tagtattaaa atgagctagg gtcgtggtag taacggttgc tgtttgggta attatattat
     6361 cttggtaggt gctgctagca aatagcttag caagatttaa attagtgcca tctggtatta
     6421 tttggagtat ccgtagccta gttccttttt ggcaattgac gctatcacag aattcagtat
     6481 taggattcca agaagataaa gcattagggt cgcaggtctc accataagcg gtattgacag
     6541 taccatcacc gcaatatcca ccgtgggtta cacaatcgct accacaaaca tattgatggg
     6601 tgggggtaga actagctggt gtcgaggtgc cataagaacc tgggtcgcag atctcaccat
     6661 aagtggcatc gacagtacca tcaccacaat atccaccgtg ggttacacaa ttggtacaac
     6721 gatattgatg ggtgagacta gaattagctg gcgtcggggt aatgtaaact ataggctcac
     6781 aggattcttc atattggacg gtgtaatctc cacaatatcc accctgtgtt acacaatcgc
     6841 taccacaaac atattgattg gtgggactag aactagctgg tttcggggta atgtaagaac
     6901 ctgggtcgca gatctcgtta tgattggcat taacagtacc atcaccgcaa tatccatcgt
     6961 aggttgcaca attggtgccg caaagatatt gatggttgga actagagtca gctggtgtcg
     7021 gggtactata agaagctggt tcacactgtt cataattact ctctacaatg ccattaccgc
     7081 aatagccacc agcaaagcga caagcattaa ttgttcctgg ttgtccgcaa tagtattgtt
     7141 gactaccacc atatgtttct tggggcataa gagtctcaga gtggtcgcat tcttcattga
     7201 ttaaactatt ggtgctagtt tggacgatgt gatctccaca atatccacca gtaaattgac
     7261 aatgaaggca accatattgt cggttaatat tagaatcgct agttcgataa gctatcccta
     7321 gtgagccatc acattgttct aggttgaggg tatagttatc tccattttgg acaatattat
     7381 ccccacacca accgccagta aagcgacagt attcattaca ggcatattgt ttataagggc
     7441 tagattcaat agcagagtga gcaataccct gttcgccgtc gcaatcttca ccagtattaa
     7501 tagcattatt accacaaagg ccaccaccga tgataatagt gcaagtcccc gtaccaatat
     7561 tatttaaact atcttgagcc gttatggtag tagtataacg tcctgtgggt ccagcgctgg
     7621 ttgcacttat tttaatatgc cgacgattat ctactaactg catacttaga ttttgccaat
     7681 tttcaagtgg ggtaaaggaa atattcggtc ttatagtatc accttcacta tcactaattg
     7741 ccgcatagcc agtgaaagct tgataagctg cccctcggag agtaggacag ttaataattg
     7801 gcccactatt actactagta cctgcacagt ttaagtttaa ggtatttaag ttttggtagt
     7861 tagattcaaa gttagcacat agccgataat ttgtactatt gatatattca tattgatagc
     7921 tacgactacc tgttggtaaa tatggccagg atgtagcaaa agaccgctgt tgttcattcc
     7981 agcaagtatt tggattatat cctggacagc tgccaaggta atttatgggg tctataggta
     8041 gagctgtatt taatttgacg gctaaagttt tctgccagct tggccatact gatagagttt
     8101 tattaataag atagctgccc gcttcaaggt tcggataatg ctggtaatta gtgcgatatt
     8161 gttccaattt atccttaata aatgttaaat caataagtcg ccgagtatcc cttattattt
     8221 tggatttaag gctattacaa tattgaccac tgggacaatc tttgttagtc aagcaattaa
     8281 ttccagaggg ggtatctggt tttgaccagc aggtaccttc ataactattg atattttggt
     8341 taaagtgcca attattaaga atctggccaa aaatatctaa agtttcattg gagacatttt
     8401 ggttaaagga aataatagca ataatcggct gtaggcattc ttttccgcca ctagcgcaag
     8461 gtactatatt agaagcagcg acatatacag ttctatcgtc tcttacagca tcataaccat
     8521 caacgataag tgactgcaat gatcgtttgg ctccaacgtt tattttgtac caatcgtaag
     8581 gacttagata gttgttatta tcaaaaatac ggacggcaat agcatcggga ttaagtgatg
     8641 gacgagctcc tgcctctatt gttgaggaag attgattagg agagccagaa tttgaagcag
     8701 cttgagtatt tggtagtcta cttattaaaa tagagaatcc gattagaatt gttataccaa
     8761 ttatgaattt tttattattt atccctaact gttgcataaa ttatcaatgc tttattatta
     8821 tagcaaaaaa gataacaaaa tccaaattta tgtgagtaaa ggcagttttt tattagtatt
     8881 ttattaatca ctttaatatt ttaatctttg gacatttttg ttatgtaatt tctgtaaatt
     8941 tatgtcggtg atattggctt ggcaaagcaa gttatatatt tcatctcgtt cggtatcagt
     9001 atatatgggt atagagtaat ttttgatttt tcttataatc tcaaaagtaa agccagcagc
     9061 atcagtgcca gagattttta gtttatctgc gtcggtaaaa attactaatg agattatagg
     9121 tgattttaat tgtgattgtc caattatttt ttctaatgct ttgatatggg catagttttg
     9181 atagaaagga ttataaaatc tttccttatt atgaaaaatc acttgagtcc aatatttttg
     9241 ttttatatta ccaaaaatcc acccagcgta ggccttagtc tctaaacaaa atataccaaa
     9301 attagagaca atgacatgat caatttgggt ggtatttaaa tttcctgtag atggtaaaag
     9361 gagattattt aatattctat aataatttga atctagtaat ttataaagct cattagccac
     9421 aaatttttcg ccttttctac ctctccgaac tggtaa
//
LOCUS       DAOOIS010000020         5966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_10833_length_5966_cov_46.249366, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000020 DAOOIS010000000
VERSION     DAOOIS010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10833_length_5966_cov_46.249366"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="PK960_02350"
     CDS             <1..1034
                     /locus_tag="PK960_02350"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HPD08040.1"
                     /translation="GSSQFYISTEDDLMRIFGGERLKRIMTTLKLPEDMPIENKIISR
                     SIESAQKRVEGNNFDLRKHLVEYDDVINKHRESIYRRRRQILELSETPVDRAMEGASL
                     LTPLSSLILEMIDNEIEQVVSFHTQSENIDDWNINEIAQVMSTILDVEATKLAQELVN
                     LGSSSTAKMDKIAYRDSLVKYLSDMVHQAYNELLALTKDIGLDWLAIEKSVLLRSIDS
                     LWMDHLDAMDYMRQGIGLRGYGQRDPLVEYKKEAYRLFNELNAAIQKQVVYAIFKVVD
                     LSRSNEQKIKQIMNIPGPAKKIVLNADGEMATAPVERKIRDSEGHKIGRNDPCPCGSG
                     KKYKKCCGR"
     gene            1045..1533
                     /locus_tag="PK960_02355"
     CDS             1045..1533
                     /locus_tag="PK960_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPD08041.1"
                     /translation="MRTKTKAVSAFKPSLGQIDYTHARWAPVINCVVKYGSKILVVKR
                     SPDLNFYPGYWNGVSGFLDDHQSLTEKVYSELKEELGLTKSQIAKIKVGDIFDQDEPK
                     YNKTWIVHPVLVEVKTDKIKLDWEASNYKWLTFPEIKKMKLLPSFAETLKRLFPFNEI
                     KL"
     gene            1530..2063
                     /gene="yjjX"
                     /locus_tag="PK960_02360"
     CDS             1530..2063
                     /gene="yjjX"
                     /locus_tag="PK960_02360"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0046128 - purine ribonucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HPD08042.1"
                     /translation="MIINIASHNPNKIEALREIIRDYDNLQPATVVDVDSLSGVSEQP
                     ISLDETIKGAINRAQESFRDCQYSVGLESGLMSVPYTQTGYMDVCACAIFDGHNYYLG
                     LSSAWEPPATVVHYIINEGLDMNEAALKAGLTPKKNVGSAEGLVGIVTKGKLSRKEYT
                     KEAIRMALIHLDKNEVD"
     gene            2133..3302
                     /locus_tag="PK960_02365"
     CDS             2133..3302
                     /locus_tag="PK960_02365"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPD08043.1"
                     /translation="MFLFSNKKIKSSLVAFTLVELLVVIAIVGILSTLSVVAFNSARS
                     KARDSRRLSDIHNLSLALEMYYNDFGAYPPAPTSTGIIGGLCLSDLGITSTCGLNTYI
                     AKIPDDPKNNKHYLYSTVVNPQHYELIYEEETNTDDCPSNWVKVPGNTLYNTRTFCVM
                     QYEAKIKGQDNGNQTYSASYEPESRPGGTPWVNITQPNAKNECQSIGAHLVTEDEWLT
                     IVRNIELQPVNWSGGQIGSGYIPRGNSDSSAAMDGTNPLSGINQRTLTLSNGEVIWDI
                     AGNVWEWTDATILGKDEPKGVANPTSFGWYQYSNLYSYGAISPERLLPLNPTWNTNQG
                     YGQIYTSGAPTNTTTYAFLRGGYWYNETPSAGVLALNLAAGASYTNSSIGFRCAR"
     gene            3364..3699
                     /locus_tag="PK960_02370"
     CDS             3364..3699
                     /locus_tag="PK960_02370"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPD08044.1"
                     /translation="MIDNLLVFQKTYDWLLWIKSATERFSRVHKYSLGQQLQIESINL
                     LKKIIIANQQKNKFAAINECLTQKEIVKVLVRLSKDYSLFDNRQYEFAAQRLLEIDRM
                     LLGWQRISN"
     gene            3994..5010
                     /locus_tag="PK960_02375"
     CDS             3994..5010
                     /locus_tag="PK960_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HPD08045.1"
                     /translation="MRKNYQSYSQIISWDNIYSAYKKAAKGRYYRQDVLIFNTKLEEN
                     LLLIRKQLETENYHFGPYQKRTIHEPKRRIISIAPFSDRIVHHAICQIIEPFFDQGFI
                     FDTYACRVNKGNQAAVKRLHHWLLGGRFTWVWQGDIYHYFSSINQQILLQEISKVFND
                     QKIVKLLEKVISSYSDDANQQIGLPIGNLTSQLFANIYLNPLDQFIKRKLKQRYYMRY
                     MDDLIILNNDKEQLRNDWLRIEYFLRDKLKLRLHPQKGQIVKIKNGFNFLGYRHFTHY
                     YRPKKETFKRFFQRIRTNVNNKNEDVKKIKDFWLSWKNYCQKAKFYFLTQRLINVVRN
                     KTMF"
     gene            5262..5564
                     /locus_tag="PK960_02380"
     CDS             5262..5564
                     /locus_tag="PK960_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPD08046.1"
                     /translation="MSKKLYVGGLSYSLTSDGLKDNFSQAGTVESATVITDKMSGRSK
                     GFGFVEMSSEEEAQKAIDMFNGQEIGGRRVVVNEARPLADRPARNNSWDHRSTHSF"
     gene            5708..>5966
                     /locus_tag="PK960_02385"
     CDS             5708..>5966
                     /locus_tag="PK960_02385"
                     /inference="COORDINATES: protein motif:HMM:NF019187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08047.1"
                     /translation="MAYTATKASTKQKGHVWFLVFLVILVVASLLINLGPQYNAFITK
                     HNIPLPKVKELPFRLGLDLSGGTSLTYDLDLSVIEPSDRLNA"
BASE COUNT         1881 a          958 c         1209 g         1918 t
ORIGIN      
        1 ctggttctag ccagttttat atttctactg aggatgattt aatgcgtatt tttggtggtg
       61 aacgtctgaa gaggattatg actaccctga aattgccaga agatatgcca attgaaaata
      121 agattatttc tcgttctatt gagtcagctc aaaaaagagt agaaggcaat aattttgact
      181 taaggaaaca cttggtcgaa tatgatgatg taattaataa acaccgggaa agtatttacc
      241 gacggcgccg acagatttta gagttaagtg aaaccccagt agacagggct atggaaggtg
      301 cttctttatt aactccttta tcttctttga ttttagagat gattgataat gaaattgaac
      361 aagtggttag ttttcataca caaagcgaga atattgatga ttggaatatt aatgaaatag
      421 ctcaggtaat gagcactata ttagatgtag aagctactaa gctggctcag gagctagtta
      481 atttaggtag cagttcaaca gctaaaatgg ataaaattgc ctatcgtgat agtctggtaa
      541 aatatttatc tgacatggtt caccaggcct ataatgaatt attagccctg accaaagata
      601 ttggactaga ttggctggct attgaaaaat cagtactttt aagatctatt gacagcctgt
      661 ggatggatca cttagatgcg atggactata tgcgtcaagg gattggcctt aggggttatg
      721 gccagcgtga tccacttgtt gaatataaaa aagaggctta ccgcttattt aatgaactta
      781 atgccgccat tcagaaacaa gtagtttatg ctatttttaa ggtggtggat ttaagccgtt
      841 ccaacgaaca aaaaattaaa caaataatga atatccccgg tccagctaaa aagattgttc
      901 ttaatgccga tggagaaatg gctactgccc cggtagaacg gaagataaga gatagtgagg
      961 ggcataaaat tggccgtaac gatccttgtc cctgcgggag cggcaagaaa tataaaaaat
     1021 gctgtggcag atagcatcta atctatgaga actaaaacca aggctgtatc agcatttaag
     1081 ccgagtttgg gtcagataga ttatacccat gctcggtggg ctccagttat taattgtgta
     1141 gttaagtatg gttctaagat attggtagtg aagcgtagcc ctgaccttaa tttttatcct
     1201 ggctattgga atggggtttc tggctttttg gacgaccatc aaagtttaac ggagaaagta
     1261 tatagtgaat taaaagagga attaggatta actaaatcac aaattgctaa aattaaggta
     1321 ggcgatattt ttgatcaaga tgaaccaaaa tataataaaa cttggattgt tcatccggtt
     1381 ttagtagaag ttaagacgga taaaattaaa ttagactggg aagctagtaa ttataaatgg
     1441 cttacttttc ccgagattaa aaaaatgaaa ttattaccaa gttttgccga aacgttaaaa
     1501 cgtttgtttc ctttcaatga aataaaatta tgattattaa tattgcttcc cataatccta
     1561 ataaaattga agctctgcgg gaaattatta gagattatga taatctacag ccagcaacgg
     1621 ttgttgatgt ggatagtctc tctggagtaa gcgaacagcc tatatcacta gatgagacta
     1681 taaagggggc aataaatcga gcccaagagt ctttcaggga ttgccagtat agcgtgggtt
     1741 tagaatctgg tcttatgtct gtgccttata cccagacagg gtatatggat gtttgtgctt
     1801 gcgctatttt tgatggtcat aattattatt tagggctttc ttccgcttgg gagccgccag
     1861 ccaccgtagt ccactatatt attaatgagg gtttagacat gaatgaggcg gcattgaagg
     1921 ctggtttaac tcctaaaaag aatgtagggt cagctgaagg attagttggt atcgtaacca
     1981 aggggaaatt atccagaaaa gaatacacta aagaagctat tagaatggcc ttaattcatc
     2041 tagataaaaa tgaagtggac tgataaaaaa aattgacgcc actagcaata agtagtaaca
     2101 tacagatgta aattattctt tgtttgtagt taatgttttt attttccaat aaaaaaatca
     2161 agtcttcact ggttgctttt actttagttg aactgctcgt agttattgcc attgttggta
     2221 tactatctac tctttctgtt gtggctttta atagtgctcg ttctaaagct cgtgactctc
     2281 gtcgtttaag tgatatccac aatctctctt tagctctaga gatgtattat aatgattttg
     2341 gtgcctatcc cccagctccc acctctactg gaataattgg tggtctttgt cttagtgatc
     2401 taggtattac ttctacctgt ggccttaata cctatattgc taaaatacct gatgatccta
     2461 aaaataataa acattatctt tactctactg tagttaatcc tcaacactat gaactaatct
     2521 atgaggaaga aaccaatact gatgattgtc cctccaactg ggttaaagta ccaggcaata
     2581 ctttatataa tacccgcacc ttttgtgtaa tgcagtatga agctaagata aaaggtcaag
     2641 acaatggcaa ccaaacatat agtgctagtt atgaaccaga atctagacct ggtggcaccc
     2701 cctgggttaa tattacccaa cctaatgcta aaaatgaatg tcaatctatt ggagctcacc
     2761 ttgtaactga agatgaatgg ctaaccatag tccgtaatat tgaactgcaa cctgtaaatt
     2821 ggtctggtgg tcaaatagga tctggctata ttcctcgagg caatagtgat tctagcgcag
     2881 ccatggatgg caccaaccct cttagcggta ttaaccagcg taccctgact ctttctaatg
     2941 gtgaagttat ctgggatata gctggcaatg tctgggaatg gactgatgct actatcttag
     3001 gtaaagatga gcctaaaggt gtggccaatc ctactagttt tggatggtat cagtattcga
     3061 acctttattc ttatggcgct atttctcctg aacgtctttt acctttaaat cctacctgga
     3121 atactaatca gggttatggc caaatctata ctagtggtgc tcctaccaat acgactactt
     3181 acgcctttct ccgtggcggc tactggtaca atgagacgcc atctgctggt gtcctagctt
     3241 tgaatttggc cgctggggcg tcttacacga attcgagcat tgggtttcgc tgcgcgcggt
     3301 agccaggggt gtcttatctt ttatctaaaa tctgtattta aattttagta ttaaaataaa
     3361 aaaatgattg ataatttgtt agtttttcaa aaaacttatg attggttgct gtggattaag
     3421 tcagcgaccg aaagattcag tcgagtgcat aaatatagtt tgggacaaca actccagatt
     3481 gaatcaataa atttattaaa gaaaataata attgccaatc aacaaaaaaa taagtttgcg
     3541 gcaattaacg aatgtttaac acaaaaagaa atagtaaaag ttttagttag gttatctaaa
     3601 gattattctt tgtttgataa tcgtcagtat gaatttgcag ctcagcgttt gttggaaata
     3661 gatcgaatgt tgttgggatg gcagcgaatt tctaattagc catttttaaa atggagggtc
     3721 ttaaataaat aaacgccttt ctccgcggtg gcaactggag caatggaacg aacgctggtg
     3781 ttttgacact caatttgaac aatggtccgg gtaattccaa caacaacatc ggttttcgct
     3841 gcgcgcgtta gccagtggtc cgaaacagaa tatttatttt taaatagata gagttttcgg
     3901 tcacccccat ttaagaaacc tgccagaatt ggcgaaatga tgcttttacc cttccgttat
     3961 tttgttaaaa tctggcggaa gggactgagc tttatgcgaa aaaattatca aagctattct
     4021 cagataatta gttgggataa tatttactcg gcctataaaa aggccgctaa aggtcgatat
     4081 tatcgtcaag acgttttaat ttttaatact aaactggaag agaacttgct tttaattaga
     4141 aaacagttag agacagaaaa ttatcatttt ggaccctatc aaaaaagaac aattcatgaa
     4201 ccgaaaagga ggataattag cattgctcct tttagcgata ggatagttca tcatgctatt
     4261 tgccaaatta ttgaaccatt ttttgaccaa ggttttattt ttgatactta tgcttgccgc
     4321 gttaataaag gaaatcaggc agcagttaaa agattgcatc actggttgtt gggcggtcgt
     4381 tttacatggg tttggcaggg tgatatttat cattattttt ctagtattaa tcagcaaatt
     4441 ttattgcaag aaattagtaa agtttttaat gatcagaaaa ttgttaaatt gttagaaaag
     4501 gtgattagct cttatagcga tgatgcgaac cagcaaattg gcctaccgat tggcaattta
     4561 accagccaat tatttgccaa tatttatctt aatccattgg atcagtttat taagagaaaa
     4621 ttgaagcaac ggtattatat gagatatatg gatgatttaa taattttaaa taacgacaaa
     4681 gaacaactga gaaatgattg gcttagaatt gaatattttt tacgggataa attaaagtta
     4741 agactgcatc cccaaaaggg ccaaattgtg aaaattaaga atggttttaa ttttttaggt
     4801 tatcgacatt ttacacatta ttatcggccc aaaaaagaaa cttttaaaag attttttcaa
     4861 agaataagga ctaacgttaa taacaagaat gaagatgtta aaaaaataaa ggatttttgg
     4921 ctgtcttgga aaaattattg tcaaaaagcc aagttttatt ttttaacgca acgattaatt
     4981 aatgtagtta gaaataaaac aatgttttag gcgcacacat ttggcgattt taattattgc
     5041 gcagagccgc attggagtct aggacttcaa tcgccctttc aatttttatt aaataacgga
     5101 cacttgtcca aaatgagctg gactgataca tttgtttgac ttttcagtaa ttatctatta
     5161 tactttttgt tgtaagtttt tctttggatg atttttaaat caagtcgccc aagaaagagg
     5221 tcgacttact tttattaaaa tgtataaatt tgtccgtttt tatgtctaaa aaattatatg
     5281 tgggcgggct atcctacagt ctaaccagcg atggcttaaa agacaatttt tctcaggccg
     5341 gtaccgtaga atcggctacc gttattactg ataaaatgtc cggtcggtct aagggttttg
     5401 gttttgtaga gatgtccagt gaagaggaag cccaaaaggc cattgacatg tttaatggtc
     5461 aggagattgg tggacgacga gtagtagtta acgaagctcg tcccttagct gatcgtccag
     5521 cccgcaacaa tagttgggat catcgttcta ctcatagttt ctaactatta tagtgtgtga
     5581 tcgtaaccac gacaccgccc tttaaggggc ggtgtttgtt tatcgttgat tttttttcta
     5641 aaatctccta tgatagaatt aaattaactt aagttaattt aattttgtta tattattaaa
     5701 atattttatg gcttatacag caacgaaagc ttccaccaag caaaaaggtc atgtctggtt
     5761 tttggtgttt ctagtaattt tagttgtggc cagtttatta attaatttag ggccacaata
     5821 taatgctttt attacgaagc acaatatacc attaccaaaa gttaaggaat tgccctttag
     5881 gctaggttta gatttgtctg gaggcactag cttaacttat gatttagatt tatccgttat
     5941 tgagcctagt gatcggctta atgccg
//
LOCUS       DAOOIS010000021         5778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_11247_length_5778_cov_38.361698, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000021 DAOOIS010000000
VERSION     DAOOIS010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11247_length_5778_cov_38.361698"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PK960_02390"
     CDS             <1..1020
                     /locus_tag="PK960_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPD08048.1"
                     /translation="EEPWQQTGRLATFDVLFKTTGSDKKKYVLAPTHEEIITPLVKKF
                     VQSYRDLPVGVYQIQTKFRNELRVKSGLLRGREFTMKDLYSFHLDQDDLNAYYLAVQK
                     AYFQILKRCGLLEQTYLTFASGGAFSQYSHEFQTVTEAGEDTIFICEDCNLAINKGII
                     KEQPVCPNCGRKDLVERRAIEVANIFKLGLKFSKAFNFTVADAKGQRQPVIMGCYGLG
                     LDRLLGAIVEVNHDNHGLIWPVEVAPFKAHLLDITTDPKGHHQAQNLYEALLKLGLEV
                     LFDDRRQTPAGSKFADADLIGCPYRLVVSDRTIEQDSFEIKRRRDTEGRLVNFDQVNT
                     YFHSN"
     gene            1043..2074
                     /locus_tag="PK960_02395"
     CDS             1043..2074
                     /locus_tag="PK960_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPD08049.1"
                     /translation="MRHNKLIRNLAIDLGTCKTMLSEPQSGLFLNEPTIVAVNKKTDQ
                     ILAVGEAAKVMVGKNPAYIEIVKPLVDGVVSDFEVTEKLLKYFIDQTQENNFFLAPKP
                     AVIIGIPLDITEVEKKAVEDAAKSAGARQVFLVEEPTLSAIGAQLPIMEATATMLVDV
                     GGGITEIAVLALGGCVAWKTLRLAGNAFDDEIIQYIREEFNLLIGEQLAEEIKIKIGS
                     AIPRPQPLTMAIRGRDLIDGLPKEIEISDEHIREALSRPVGQIIDNIKAALEVTPPEL
                     VADIYERGIMLTGGSALLNGLDQAIARATKIGVHIVDDPISTVVRGAAHLLSDSELLN
                     KIVVPSLAE"
     gene            2077..2886
                     /gene="mreC"
                     /locus_tag="PK960_02400"
     CDS             2077..2886
                     /gene="mreC"
                     /locus_tag="PK960_02400"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPD08050.1"
                     /translation="MSRQRPKKSIWWLGIIIVLIIAHFLGWLSKPENIISRGLGAVVF
                     YVAKVPQATSNWWQTWQQHQVCQQELHQAQDQIKELNLSMLSYANLVEENQKLRDLLN
                     FQQLGGHKLVTANIIYRGRVGGLLAPDQTFIIDKGTNQGLALDEVAVDERGAVVGKIG
                     QIKGDSAEIWLTTNKNCRLAVTVTSSSETIGVTQGDLGLTIQVDLIPLQLNLYPGQEI
                     ITSGLEPTVPKNLVVGKISQVLKADNALWQSAIVEPAVNFSQLNFVSVIVP"
     gene            2892..3392
                     /locus_tag="PK960_02405"
     CDS             2892..3392
                     /locus_tag="PK960_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08051.1"
                     /translation="MRRSIVVKLLWLGIVVILQLAWINQWPSTWPQPDLILVTLIFWL
                     FAAGWSETKWWVVGTALILGAASFYPFAFYLILWLAVFYLLYYLLLKIFTNKSIYSLM
                     ALVALGTVGQYLGFMLLGNSWSSSLWWLILGKELIANLLIAGFIYYLLRWRDKRLVPL
                     FMVKDE"
     gene            3394..5403
                     /gene="mrdA"
                     /locus_tag="PK960_02410"
     CDS             3394..5403
                     /gene="mrdA"
                     /locus_tag="PK960_02410"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPD08052.1"
                     /translation="MAKKGQQIKNLNSPFGLVSEKKRQTHLSSGHKEWTEEVFLNEAK
                     AEETVSHNFNPHFGLILLRILLLAGLILLARVVWLQFYRGDYYYNLAEGNRLRTERLE
                     AKRGIIYDTHHEPLVSNMANFIAYVTPSNLPTDAGLRQAVLAQLSLILGESAITSLKE
                     ELAVIDPGSADFYQPVVISDNLTYNEVVQLTIKLHRLPGVSLATTNKRLYANLSNWLA
                     TTSSTLSLNSLSLILGYTGKISPTELEKYKESGYSPLDYIGKVSLEAYYETDLRGTNG
                     SKQVEVDALGNEKAIISQQNPIDGRHLVLNIDARLQAKLEEIMRQHLARIGGRRAAAI
                     VINPQNGAILAMVSQPSFDNNSFSLGIKQADYDALKNDPDTPLLNRAISGEYPSGSTI
                     KPIIAAAALEEKIITPSTSFLSTGGLRVGQWFFPDWKAGGHGWTNVTKAIAESVNTFF
                     YYIGGGYEDFTGLGVERLGQYLQSFGLGRPTGIDLTGETSGFVPTAEWKEQTTGEPWY
                     IGDTYHLAIGQGYLLVTPLQVANYTTAFANGGRIYQPRVVQEILNNDGSIYEVKEPKI
                     IAENMVSPENIETVRQAMRQTVVSGSAQSLKSLPVAVAGKTGTAQFDSRKPSHSWFTG
                     FAPFDNPQMVLTVLVEEGGESTDSAVPIAKDFWQWYFSSLDTSGG"
     gene            5440..5700
                     /locus_tag="PK960_02415"
     CDS             5440..5700
                     /locus_tag="PK960_02415"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HPD08053.1"
                     /translation="MATNNSLTSGRTTEQRLNIIIGQLNGLKKVIGQSNYDCSALITQ
                     VKAVKAGLDSFLEKILEEQLDRCLKATKSKPEVKKIFNQVIR"
BASE COUNT         1639 a          965 c         1349 g         1825 t
ORIGIN      
        1 gaagaaccat ggcagcaaac tggccggtta gctacttttg atgttttatt taagactacc
       61 ggcagtgata agaagaaata tgtgctagca ccaactcatg aggaaattat tactccgcta
      121 gtaaaaaaat tcgtccagtc ttaccgtgat ttaccggtag gggtgtacca aattcaaact
      181 aaatttcgca atgaattaag ggttaaatca ggtttactgc ggggtcgaga gtttactatg
      241 aaggaccttt actcttttca cttagaccag gacgatctta atgcctatta tctagcagtt
      301 cagaaggctt attttcaaat tttaaaacgt tgcggccttt tagagcaaac ttatctaact
      361 tttgcctcgg gtggagcctt tagtcaatat tctcatgaat ttcagacggt aactgaggcc
      421 ggtgaagata ctatttttat ttgtgaagat tgtaatttag ccatcaataa agggattatt
      481 aaggaacagc cagtttgtcc taattgtggt cgcaaggatt tagtggagcg acgggcgatt
      541 gaagttgcta atatttttaa attaggctta aaatttagta aggcttttaa ttttaccgta
      601 gctgatgcta agggtcagcg acagccggtt attatgggtt gttatggttt aggtttggat
      661 agacttttag gggcgattgt tgaggtgaat catgataatc atggtttaat ttggccagtt
      721 gaagtggcgc cctttaaggc tcatttatta gacattacta ctgatcctaa gggccatcat
      781 caagcacaaa atctttatga ggctttactt aaactaggac tggaagtgct gtttgacgat
      841 cggcgtcaaa cgccggctgg ctctaagttt gctgacgccg acttaatcgg ctgtccttat
      901 cgtctggtag taagtgaccg gaccattgaa caagacagtt ttgaaattaa acgccggcga
      961 gacactgaag gacggctagt taattttgat caagttaata cctattttca tagtaattaa
     1021 acaataattc atgtttcata aaatgagaca taataaatta atacgcaatc tggccattga
     1081 cctggggact tgtaagacca tgttaagtga accgcaaagt ggtttatttt taaacgagcc
     1141 aactattgta gcggttaata aaaaaactga tcaaatttta gctgttgggg aagcggccaa
     1201 ggttatggtg ggtaagaacc cggcctatat tgagattgta aaacctttgg ttgatggtgt
     1261 agtctctgat tttgaggtaa ccgaaaaact gcttaaatat tttattgacc agacgcaaga
     1321 aaataatttc tttttagcgc ccaagccagc tgttattatt ggtattccgc tagacattac
     1381 tgaggtggaa aagaaggcag tagaagatgc tgccaaatca gctggggcac gacaggtttt
     1441 tttggtggaa gagccaacgc tctcagctat tggggctcaa ctgcccatta tggaagctac
     1501 tgctacgatg ctggttgatg ttgggggtgg tattactgaa attgccgttt tagctttagg
     1561 tggttgtgtt gcctggaaga ctctgagact ggcaggtaat gcttttgatg atgagattat
     1621 tcaatatatt agagaagaat ttaatttatt aattggtgaa caattagcgg aagaaattaa
     1681 aattaaaatt ggttcagcca tcccgaggcc tcagccactg accatggcta ttcgcggccg
     1741 tgatcttatt gatggcttgc cgaaagagat agaaattagt gatgagcata tccgtgaagc
     1801 tttatctcgg cctgtcggtc aaattattga taatattaag gcggctctag aagttactcc
     1861 gccggagtta gtcgccgata tctatgagcg ggggattatg ctgaccggtg gcagcgcctt
     1921 gcttaatggt ttagaccagg ccattgctcg agctactaaa attggcgtcc atattgttga
     1981 tgaccctatc agcacggtag tgcgtggggc cgctcattta ctaagtgatt ctgaattatt
     2041 gaataaaatc gtggtgccta gtttggctga ataaatatga gtcggcaaag gcccaaaaaa
     2101 tctatctggt ggttagggat tattatagtc cttattattg ctcacttttt aggctggctg
     2161 tctaaaccag aaaatattat tagtcgtggt ttaggggcag tggtttttta tgtggctaag
     2221 gtacctcagg ctacgagtaa ctggtggcaa acttggcaac agcatcaagt ttgccagcag
     2281 gaactgcacc aagctcaaga tcaaattaag gaattaaact tatctatgtt gagttatgct
     2341 aatttggtcg aagagaatca aaaattacga gatttattaa attttcaaca attaggtggt
     2401 cataaattag taactgctaa tattatctat cggggacggg taggcgggtt attagcgcct
     2461 gatcagactt ttattattga caaaggtact aatcaaggtc tagctcttga tgaagtagcg
     2521 gtggatgaac ggggggcggt agtaggtaaa attggccaga ttaagggaga ttcagccgag
     2581 atctggttaa cgactaataa aaattgccgc cttgcagtta cggtcaccag cagtagtgag
     2641 actattgggg taactcaggg cgatttggga ttaactattc aggtagattt aattcctttg
     2701 cagcttaatc tttatcctgg acaggaaatt ataacctcgg gtttagagcc aacagtgcct
     2761 aaaaatttag tagttggcaa aatttctcag gttcttaagg cggataatgc tttatggcaa
     2821 agcgctatag tagagccagc ggtcaatttt agtcaattaa attttgtgag tgttattgtt
     2881 ccttaattat tatgcgacgg tctatagtgg ttaaattatt atggttaggg atagtagtga
     2941 ttttgcaatt agcctggatt aatcaatggc cgagtacttg gcctcagcca gatttaattt
     3001 tagttactct tattttttgg ctatttgcgg ccgggtggtc agagacaaaa tggtgggtag
     3061 taggtacagc gcttatttta ggtgctgctt cgttttatcc ttttgctttt tacttaatat
     3121 tatggctggc agtcttttat cttctttatt atttgcttct gaagattttt actaataaat
     3181 ccatttattc tttaatggct ttagttgctc taggtacggt gggacaatat ttgggattta
     3241 tgttattagg gaacagctgg tcgtcttctt tgtggtggct tattttaggt aaagagctaa
     3301 tagctaattt attaatagct gggtttattt attatttact gcgttggcga gataagcggt
     3361 tggtaccgct ttttatggtt aaggacgagt aatatggcca agaaaggtca acaaattaag
     3421 aatttgaaca gcccatttgg cttagtaagt gagaagaaac gtcagacaca tttaagttca
     3481 ggacacaaag aatggactga agaggttttt ttaaatgaag ctaaagccga ggagactgtt
     3541 agccataatt ttaatcctca ttttggtctt atactcttaa gaattttatt actagccggg
     3601 cttattctat tagcgagagt agtatggcta caattttacc gtggggatta ttattataat
     3661 ttggctgagg gtaatcggtt gcgcacggag cgtttagagg ctaagcgagg aattatttac
     3721 gatacccatc acgagccttt agtaagtaat atggctaatt ttatagctta tgttaccccc
     3781 agcaatctgc ctactgacgc tggtttgcgt caggcagtat tagcccagct atctctaatc
     3841 ttaggagagt cagccataac aagtcttaaa gaagagttag ccgttattga tcctggctca
     3901 gccgattttt accagccagt agttattagt gataatctaa cttataatga agtggttcag
     3961 cttactatta aacttcatcg gttgccggga gtatctttag ctactactaa taaaagatta
     4021 tatgccaatt tatccaattg gctcgccact actagttcga cgttgagctt aaatagtcta
     4081 agtcttattt taggttatac tggtaagatt agtccaaccg aattggagaa atataaagag
     4141 agtggttatt cacccctcga ctatatcggt aaggttagcc tagaggctta ttatgaaact
     4201 gacttgcgag gtacaaacgg cagtaagcaa gtggaagttg atgcgctggg taatgagaaa
     4261 gccataatta gtcagcaaaa tccaatagat ggccgccatt tagttttaaa tattgatgct
     4321 cgtctccaag cgaaattaga ggaaattatg cggcaacatt tagcgcggat tggcggacgt
     4381 cgggcagcag ctattgtcat aaatcctcaa aatggagcta tattggcgat ggtcagccaa
     4441 cccagttttg acaataatag tttttcgtta ggtattaagc aggctgatta tgatgccttg
     4501 aaaaatgacc cagatacacc cttacttaat cgggctatca gcggggaata tccttcaggt
     4561 tcaactatta aacccattat tgctgcggct gccctggaag aaaagataat tacccctagt
     4621 accagttttt taagcactgg cggtcttagg gttggtcaat ggttttttcc tgactggaaa
     4681 gctggtggtc acggctggac taacgttact aaggctattg ctgaatcagt taatactttt
     4741 ttttattata ttggcggtgg ctatgaagat tttactgggt taggagtaga acgtcttggt
     4801 cagtatttac agagttttgg tttgggccga ccgaccggca ttgatttaac tggtgaaact
     4861 tcgggttttg tgcctacagc cgaatggaaa gagcagacca ctggtgagcc atggtatatt
     4921 ggtgacactt accatttagc tattgggcag ggttatttat tggttactcc actccaggtg
     4981 gccaattata ctaccgcttt tgctaatgga ggaagaattt atcagccacg tgtagtccag
     5041 gaaattttaa ataatgatgg ttcaatttat gaggttaaag aacctaaaat tatcgccgaa
     5101 aatatggtga gtccggaaaa tatagagact gttagacaag cgatgcgtca gaccgtagtc
     5161 tctggtagtg ctcagagtct gaagtctttg cccgtggcgg tggccggcaa gactggtaca
     5221 gcccagtttg atagccgtaa gccatcgcat tcatggttta ctggctttgc tccctttgat
     5281 aacccccaaa tggttttaac tgttttagtg gaagaggggg gtgaaagcac tgattcggct
     5341 gtacctattg ctaaggattt ttggcaatgg tatttttcat ctcttgacac ctctgggggg
     5401 taatgtataa tgaagaagtt aataaattta agatttttta tggctactaa taattcttta
     5461 actagcggac gtactactga acagcggttg aatattatta ttggtcaact aaatggctta
     5521 aagaaagtca ttggccaatc taattatgac tgctcggctt taattactca agttaaagca
     5581 gtgaaagctg gtttagattc attcctggaa aaaatattgg aagaacaact agatcgttgt
     5641 cttaaggcga ctaaaagtaa accagaagtt aaaaaaattt ttaatcaagt tattcgttaa
     5701 tttctaattt atggcacaga ttgtagaatt aacgccggct aattttgcca ctgaggtttt
     5761 aaatagcaaa ataccggt
//
LOCUS       DAOOIS010000022         5760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_11287_length_5760_cov_36.836284, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000022 DAOOIS010000000
VERSION     DAOOIS010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11287_length_5760_cov_36.836284"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PK960_02420"
     CDS             complement(<1..592)
                     /locus_tag="PK960_02420"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HPD08054.1"
                     /translation="MTTKPFPQPIPFLKMLGPSFVILALGLGSGEIILWPYLTANYGL
                     GIVWGALLGLTCQYFINMEIERYALVKGESVFTGLAKLWPKLPYWFIISTFLGWALPG
                     IAAASAQIFGHLIGLKDFRWLAMIILLAIGLILTLNQSVYNTLEKITKTIILIGVPCL
                     IVLAIILAVKTDWLALFQGLIGRGYNYHWLPSGVGLA"
     gene            655..1851
                     /locus_tag="PK960_02425"
     CDS             655..1851
                     /locus_tag="PK960_02425"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPD08055.1"
                     /translation="MSEPLASLMRPTKLEDFVGQEDIVGPGSFLRQALANKHVPSLLF
                     WGPPGCGKTTLAKIIAAELDSEFFALSAVTSGLKELRQVIDQARSNQLLGKKTLLLVD
                     EIHRWNRGQQDALLPYVENGTIILIGATTENPSFSVNGALLSRCQVIVFKQLSVDNII
                     ILLKRALSDKRRGLGGRVKADKPALRRIAHLADGDARLALNLLEAAAKTESAITLEVV
                     DKISRQIVFNYNQVGDERYHLISALHKSMRGHDADAAVYWLARMLEAGEDPLYIARRL
                     VRFASEDIGLANNTALLLATSCFEACRQIGLPECKVILAQTVIYLAKSPKSIAAYEAY
                     GRAVADVQQFGALPVPLHLLNASTKLAKELGYGEGYKYTPKEDSSDQNYWPQKLSGGR
                     QYLPHK"
     gene            complement(1852..3018)
                     /locus_tag="PK960_02430"
     CDS             complement(1852..3018)
                     /locus_tag="PK960_02430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPD08056.1"
                     /translation="MLKNKSTFHIIYALAFAATIANAITGYVQSSYLNHFLSLNYIGL
                     FLSLVTIITIIISFIYPKLISRHSVYSLTILTLFLSIIASLFMSSGTNSLLILIAFTI
                     RYLSLTFLMINLDIFLEDISTDNRTGVIRTQYLTVMNVAWLMSPLLMGHIIGPNNDYP
                     QIYYIGGLILLAAFILLIFTKHQLPRSKIYCQEQSLSKGLNKLIGHGDRRRIFFSSLV
                     LQFFYALMTLYVPIYLNQDVGLSWTTLSIIFTIMLIPFVVLELPAGALADKLLGEKEI
                     LLVGNIIMIISVVGIFLAKTANPFVWGFLLFCSRVGAALAESMQESYFYKKIDSHDTA
                     VINLYRQNRSLGWLLASLTAFITLSFCSLPAIFIVLAISLTLGLIPILGIHDTR"
     gene            3120..4043
                     /locus_tag="PK960_02435"
     CDS             3120..4043
                     /locus_tag="PK960_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HPD08057.1"
                     /translation="MPKLLSQLVYPADVPKYRRTIYNTNWCLLTKNTGRLLLLAGDQR
                     LEHLNNDFFGPGIDSADNNPEHLFQIAVHAPIGALAIHYGLASRYGYNYRQVNYVIKL
                     NGKTNLISDDPDSHLLATIDQTIELSKEAGFHLVGVGYSIYPGSRYEPRMLAEASQII
                     IEAHDNGLPVILWIYPRGGKIKNEDDVSLIAGAAGVAVSLGADFVKVRAPKPLTMKNI
                     HRVVEAAGLTKVIFAGGANTSHDKLWHDVSLELKGGSAGLAVGRNIHQYSVEEARERL
                     QVLAALIYENKTLAQATTVFNKKRHAKNNLK"
     gene            4048..4359
                     /gene="raiA"
                     /locus_tag="PK960_02440"
     CDS             4048..4359
                     /gene="raiA"
                     /locus_tag="PK960_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPD08058.1"
                     /translation="MQANIKANNFELTPAIKSYVEKKLNMLDKYLGSIVPISCDVEVE
                     RTTRHHWKGDVFRAEINLELPGELLRIEKVEPNLYKAIEKAKDRLAPMIVKYKEKKQK
                     C"
     gene            4431..>5760
                     /locus_tag="PK960_02445"
     CDS             4431..>5760
                     /locus_tag="PK960_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPD08059.1"
                     /translation="MGFFQKIVGDPNERVIKSLQPTLNLINEWESRVADKTQEELQQR
                     TQEFRAQYSAIAKEKDLKEAENYLDEILPEAFAMVREVSKRTLGQRHFDVQLIGGIIL
                     HRGEIAEMKTGEGKTLVATLPLYLNALSGQGVHLVTVNDYLARIGAGWMGPIYNYLGV
                     SVAVIIHDQALQYDPTYNDESQYDERLRHFRPVSRQAAYACDVLYGTNNEFGFDYLRD
                     NMVYELSQMVQRPLHYAIVDEVDSILIDEARTPLIISTPAEESTDKYYKFAELVARLD
                     EATDYNVDEKMRAATLTEAGIAKMEKWLGVDNIYVEGGVDDVMHIEQALKARVLFKKD
                     RDYVVKDGEVIIVDEFTGRLMYGRRYNEGLHQAIEAKEGVVIQKESRTLATITFQNYF
                     RMYHKLAGMTGTALTEAEEFSKIYHLETVVIPTNKPTIRQDLNDLIYATEE"
BASE COUNT         1847 a          961 c         1282 g         1670 t
ORIGIN      
        1 tagctaaacc aacacccgat ggcagccagt gataattata tcctcggcca atcagaccct
       61 gaaatagagc gagccagtca gttttgacag ctaagatgat tgctaaaaca attaaacagg
      121 gtactccaat taggataata gttttagtaa ttttttccaa agtattgtaa acactttggt
      181 ttagagtaag aattaagcca atggccaaca aaataatcat agccagccag cggaaatctt
      241 taaggccaat aaggtgacca aaaatctggg cactggcggc ggcaatccct ggcaaagccc
      301 agcctagaaa agttgaaata ataaaccaat aggggagttt aggccagagc ttagctagac
      361 cggtaaaaac actttcacct tttactaaag cgtaacgttc aatctccata ttaataaaat
      421 attggcaggt gagaccgagc aaggcgcccc agacaattcc tagtccatag ttagcagtaa
      481 gatatggcca gagaataatt tctcctgagc ctagtcctaa ggctaagatg acaaaacttg
      541 ggccaagcat cttgaggaaa ggaatgggtt gcggaaaagg tttagtcgtc atacaattaa
      601 taaagataat tttattttat tttagcagaa aaagttttga ttaacaaaaa atatatgtct
      661 gaacccctcg ctagccttat gcggcccacc aagttggaag attttgttgg ccaggaagac
      721 attgttggtc ctgggtcatt tttacggcaa gcgttagcta ataagcatgt gccatcgtta
      781 ttattttggg gaccacctgg ctgtggcaaa accactttag ctaaaattat tgccgctgaa
      841 ctggattctg agttttttgc tttgtcggcc gtaaccagcg gcttaaaaga attacggcag
      901 gttattgacc aggcccgcag taatcaactg ttgggtaaga agactctttt actggttgac
      961 gaaattcacc gttggaatcg tggccagcaa gatgctttac tgccctatgt agaaaatggc
     1021 acgattattc tcattggcgc gactaccgaa aatcctagtt tttcagtcaa cggcgcttta
     1081 ctgtcgcgct gtcaagtcat tgtttttaag caactttcgg tagataatat tataattttg
     1141 ttgaagagag ccttatcaga taaaaggcga ggattggggg ggcgagttaa ggcggataaa
     1201 ccagccttaa ggcgtatagc ccatttggct gatggcgatg ctcgtttggc tcttaattta
     1261 ttagaagctg ccgctaaaac tgaatcagcc atcactttag aggtggtaga taagattagc
     1321 cggcaaattg tctttaatta taatcaggtg ggtgatgaac gttatcactt aatttcggct
     1381 ttacataaaa gcatgcgtgg tcatgatgcc gatgcagcgg tttattggtt agccagaatg
     1441 ttggaagcgg gtgaagatcc tttatatatt gctcgccgtc tagtacgttt tgctagtgaa
     1501 gatattggtt tagctaataa tacggcctta ttgctagcta ccagttgttt tgaagcctgt
     1561 cggcagatag gactgccaga atgtaaagtt attttagctc agacagttat ttatttagct
     1621 aaatcaccca aaagtattgc ggcttatgag gcttacgggc gggccgtagc tgatgtccag
     1681 caatttgggg ctttgcctgt gccgcttcat ttactaaatg ctagtactaa actagccaaa
     1741 gaattaggtt atggcgaggg ttataaatat actcccaaag aagatagttc agaccagaat
     1801 tattggccgc agaagctatc gggcggtcgt cagtatttac cgcataaata atcagcgggt
     1861 atcatgaata cctaatatag gaataagtcc aagggtaagg ctaatagcga ggactataaa
     1921 aatagcgggt aaagaacaga aacttaaagt gatgaaggct gtaagggaag ctagaagcca
     1981 gcctaaagaa cgattttgac gataaagatt aatgacagca gtatcgtggc tgtcaatttt
     2041 tttgtagaaa taagattctt gcatactttc ggccagcgcc gcgccaaccc gactgcaaaa
     2101 caataaaaat ccccaaacaa aaggattagc ggttttggct aaaaatatac ccactaccga
     2161 gataatcata ataatgttac ctactaggag tatttctttt tcgccgagaa gtttatccgc
     2221 taaagctcca gctggcagtt ccagaacaac aaaggggata agcataatag taaaaataat
     2281 acttaaagtt gtccagctta gtccaacatc ctggttaaga taaatcggaa cataaagggt
     2341 cattaaggca taaaaaaatt ggagtactag agaagagaaa aaaatacggc gacgatcacc
     2401 atgtccgatt aatttattaa ggcccttact aagactttgt tcttggcagt aaattttaga
     2461 acggggtagt tggtgtttag taaaaatgag taaaatgaaa gctgccagta aaattaagcc
     2521 accaatataa taaatttgtg gataatcgtt gttagggcca ataatatgac ccataagtag
     2581 cggtgacatt agccaggcga cattcataac tgttaggtat tgagtcctaa ttacgccggt
     2641 acggttgtcg gtagaaatat cttctaggaa aatatccaga ttaatcatca gaaaagtaag
     2701 agatagatag cgaatagtaa aagctattaa tataagtaaa gaatttgttc cagaagacat
     2761 gaatagacta gcaataatag acaggaataa agtgaggatg gttagactat aaactgaatg
     2821 acgggagatt agtttggggt aaataaatga gataataata gtgataatag ttaccagtga
     2881 caaaaataag ccaatataat ttaaggacag aaaatgattt aaataagaag actggacata
     2941 gccagtgatg gcattagcaa tagtagcagc aaatgccaag gcataaatta tatggaaggt
     3001 ggatttattt tttagcataa aaaatagccc tataacgact tgcttttatt atataaaaaa
     3061 ttaggaataa agccaagttt atgttataat tttaataaat tagtctttat tttattatta
     3121 tgcctaaatt attatctcag cttgtatatc cagctgatgt tcccaagtat cggcggacca
     3181 tttataatac taattggtgc ctgcttacta aaaatactgg ccgtctattg cttttagctg
     3241 gtgatcagcg gctagagcac cttaataatg atttttttgg accaggcatt gattcagctg
     3301 acaataatcc agagcatctt tttcaaatag cggttcacgc cccaattggt gctttggcca
     3361 tccattatgg gttagccagt cgttatggct ataactatcg gcaagtaaat tatgtcatta
     3421 agcttaatgg taagactaat ttaattagcg atgatcctga cagtcattta ctagctacta
     3481 ttgatcaaac gattgaatta tccaaggagg ctggttttca cttagttggt gttggttatt
     3541 ccatttatcc gggaagccgt tatgagccga gaatgctagc ggaggcaagc caaattatta
     3601 ttgaagctca tgataatggt ttgccggtta ttttgtggat ttatccacgt ggaggtaaga
     3661 ttaaaaatga agacgatgtt tctctcattg ctggagcggc tggagtagcg gttagcttag
     3721 gagctgattt tgttaaagtt agagcaccta agcctttaac tatgaaaaat attcaccggg
     3781 tagttgaagc tgctggcctg actaaagtta tttttgccgg tggtgctaat acttcccacg
     3841 ataaattatg gcatgatgtt agtttagagt taaaaggtgg tagtgctgga ttagcagtag
     3901 gtcggaacat tcatcagtat tctgtagaag aggcgaggga gcgattgcag gttttagctg
     3961 ccttaattta tgaaaataag accttggctc aagccactac ggtgtttaat aaaaaacgac
     4021 acgctaaaaa taatttaaaa taaatatatg caagccaata tcaaagccaa taattttgaa
     4081 ttaaccccag ctattaaaag ttatgttgaa aaaaaactta atatgctgga taaatattta
     4141 ggaagcattg tgccgattag ttgtgatgta gaggtagaaa gaactactcg tcaccactgg
     4201 aagggcgatg tttttagggc tgaaattaat ctagagttgc cgggagaact tttaagaatt
     4261 gaaaaagttg agcctaatct atataaggcc attgaaaaag ctaaagatcg tttggcgccg
     4321 atgattgtta aatataaaga aaaaaaacaa aaatgttagc cttgcatttt gtttaatttt
     4381 ctctctataa taaaaaacta tctcagagaa ttatttattg aaaaatattt atgggttttt
     4441 ttcagaaaat agttggtgac cctaatgaac gggttattaa aagtttacag ccaacactta
     4501 atttaattaa tgagtgggag tcacgggtag ccgataaaac tcaagaggaa ttgcaacaac
     4561 gaactcaaga atttcgagcg caatattctg ctattgccaa agagaaggat cttaaggagg
     4621 cagaaaatta tttagatgaa attttgccgg aagcatttgc gatggtgcgc gaagtaagta
     4681 aacgcacctt aggtcaacgg cattttgatg ttcagctaat tggcggtatt attttacatc
     4741 gtggagagat tgctgaaatg aaaactggtg aaggtaaaac tttagtagcc accttaccgc
     4801 tttatttgaa tgctttaagt ggtcaagggg ttcatctggt gacagttaat gattatttgg
     4861 ctcgtattgg cgcgggatgg atgggcccaa tctataatta tttaggtgtt agcgtcgcgg
     4921 taattattca tgatcaggca cttcaatatg accctaccta taatgatgag agtcaatatg
     4981 atgagcggct taggcatttt cggcccgtta gccgtcaggc ggcttacgcc tgtgatgtct
     5041 tatacggtac taataacgaa tttggctttg actatctgcg agataatatg gtttatgagc
     5101 ttagtcaaat ggtccagcgg ccgcttcatt atgcgattgt cgatgaagtt gactctattt
     5161 taattgatga agcgagaacc ccattaatta tttccactcc agctgaagaa tctacagata
     5221 aatattataa atttgcggaa ttagtggctc gtttggatga ggctactgat tataatgttg
     5281 atgaaaaaat gcgcgctgct actttaactg aagcaggcat tgctaaaatg gaaaaatggt
     5341 tgggggtaga taatatttat gttgagggcg gggttgatga tgttatgcat atcgagcaag
     5401 ccctaaaagc cagagtacta tttaaaaaag accgtgatta tgtcgttaag gatggtgaag
     5461 ttattattgt tgatgaattt accggacgtt taatgtacgg ccgtcgctat aacgaagggc
     5521 ttcatcaggc cattgaagcc aaagagggag tagtgattca aaaagaaagt cgtactctgg
     5581 cgaccattac ctttcagaat tattttcgca tgtaccataa gcttgccggt atgaccggca
     5641 cagctctaac cgaagcggaa gaattttcta aaatttatca tctagagacg gtggttattc
     5701 ctaccaataa gccaacgatt cgtcaggacc ttaatgattt gatttatgcc accgaagaga
//
LOCUS       DAOOIS010000023         5371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_12239_length_5371_cov_39.036494, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000023 DAOOIS010000000
VERSION     DAOOIS010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12239_length_5371_cov_39.036494"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /gene="recA"
                     /locus_tag="PK960_02450"
     CDS             <1..893
                     /gene="recA"
                     /locus_tag="PK960_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPD08060.1"
                     /translation="VDAVSSGCLSLDLALGVGGVPRGRIIEIYGPESSGKTTLAQHII
                     AEVQKQGGIAAFIDAEHALDPEYAKKIGVNIDEMLISQPDTGEQALEILETLVRSNAV
                     DVVVVDSVAALVPKKEIEGDMGDLQMGSQARLMSQALRKLTGVISKSKTIVIFINQLR
                     MKIGVMFGNPETTTGGTALKFYASVRIDIRKIAQIKQGDKMIGNRVKCKIVKNKVAAP
                     FKSCEFDIMYNEGISIAGDLLDTGVQRGTVTKTGNSYSFGEIKLGVGRENAKAFLKEH
                     PELMSQIREQILADIAAKGE"
     gene            complement(984..1553)
                     /gene="efp"
                     /locus_tag="PK960_02455"
     CDS             complement(984..1553)
                     /gene="efp"
                     /locus_tag="PK960_02455"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPD08061.1"
                     /translation="MLTLNEIKPGKVINVNGEPYTIIRADHHKMGRGGAVLKTKLRNL
                     ITGAVLEKTWQGNDRAEEADVEKRKATFMYLDSQFANFMDSETYEQFAVNLEDLGGKE
                     KFLKDGLEVDVLYFQGRPVTVELPIKVEYKVTQAPPGVRGNTAGNVMKQIELETGAKI
                     NAPLFINEGDIVRVNTDTEEYVERINESK"
     gene            complement(1559..1777)
                     /gene="rpsR"
                     /locus_tag="PK960_02460"
     CDS             complement(1559..1777)
                     /gene="rpsR"
                     /locus_tag="PK960_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPD08062.1"
                     /translation="MNHYTSGKPCYFCHNNIKEIDYKDTFLLRRYTNSLGKILPKRRT
                     GTCDKHQRQLALAIKRARIMGLLMFTRQ"
     gene            complement(1789..2238)
                     /locus_tag="PK960_02465"
     CDS             complement(1789..2238)
                     /locus_tag="PK960_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPD08063.1"
                     /translation="MDLNKVMLIGNLTRDPEIRSTASGQPVASFTVVTNLVWTDQTGQ
                     RQEKAEFHNVVAWKRLADICRQYLHKGSKVYVEGRLQTRDWVAQDNTKRYRTEVIADN
                     IIMLDRAGGTTGPFTPPPSEGEESELPTESIPENPEEEEIKIEDIPF"
     gene            complement(2270..2749)
                     /gene="rpsF"
                     /locus_tag="PK960_02470"
     CDS             complement(2270..2749)
                     /gene="rpsF"
                     /locus_tag="PK960_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPD08064.1"
                     /translation="MAKNKSSQIPRYELLYIVSNKFTSEETEPIAQKVEDLITSNNGT
                     IVYRENWGKRHMSYPIKGFQFGYYRLLEFDLPANKIEEINRYLRLSNDILRHQIIKRQ
                     PRTVEEILAAKEEIAKREAKIKAGEIDINQKSKPEAPSEQNLNETQTTNTVEEPTVV"
     gene            2968..4131
                     /locus_tag="PK960_02475"
     CDS             2968..4131
                     /locus_tag="PK960_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPD08065.1"
                     /translation="MNYFFILGNHPALSLSELSVKIAGNWQIVNDAVVTLHYDGELAA
                     NLLESLGGTIKFGKIMGQFKKLDEVAEEDLASYLLSLNQGDGKIHFGLSNYSSIVLDR
                     SFGLQIKRRLQVAGRSARWVVGRDKVLSSVIVEQNHLLTKGKEIVFWRYNDYWQWGIT
                     IGVQPFKGLSARDYGRPARDDRSGMLPPKLAQIMINLAGLRPGDILLDPFCGSGTILQ
                     EAALLGASKVIGIDNAPQAIKNSQDNWAWFAQRWRVNTSAEFRLGEATKVSQILSSKI
                     NRIVTEPYLGPSRGPRNFPAIIKELEVLYSQALRDWRSVITKEGRVVMIWPIFLCGTK
                     KYYVQPDISGWQTIEFSKLARQSWPQEYTPGGHLIYGRSRQTVWRAIVGLQPV"
     gene            complement(4128..5186)
                     /gene="ychF"
                     /locus_tag="PK960_02480"
     CDS             complement(4128..5186)
                     /gene="ychF"
                     /locus_tag="PK960_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPD08066.1"
                     /translation="MTLSLGIIGLPNVGKSTLFNALTKKQVDAANYPFCTIEPNVGVV
                     KVPDERVTKLAAMSNSAKQIYATIEFVDIAGLVKGAHEGEGLGNQFLANIRNVDAICH
                     VIRNFQDSNVVHVEGRINPSLDKEIIDLELIYADLATVNRRLDKISKEAKSGDKLISK
                     QVDTLKKIRDYLNEGQSLRRCSLTADEQELAKELNLLTSKPVIYVINSEEPANLLLHL
                     DGPAIVLNAKLEAEIASLPEEEQADYIKELGLQDSGLNKLIAQAYNLLGLITFLTTGR
                     DETRAWTVKRNTPAPQAAGVIHTDFMKNFIRAEVINWETLLRAGSETKAKELGLIRLE
                     GKNYLIQDGDVCNFLINN"
     gene            5288..5361
                     /locus_tag="PK960_02485"
     tRNA            5288..5361
                     /locus_tag="PK960_02485"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5322..5324,aa:Asp,seq:gtc)
BASE COUNT         1456 a          969 c         1184 g         1762 t
ORIGIN      
        1 atgtagatgc tgtctctagc ggttgcctct cattagattt ggccttgggg gtcggcgggg
       61 tgccacgggg tcggattatt gaaatttatg gcccagagtc tagcggtaag actactttag
      121 ctcagcatat tattgccgag gtgcaaaaac agggcggtat tgctgccttt attgatgccg
      181 agcatgctct agatccagaa tatgctaaaa aaattggggt gaatatagac gagatgttga
      241 ttagtcagcc agatactggt gagcaggctt tagaaatttt agagacattg gttcgctcta
      301 atgcagttga tgttgtggtg gttgacagtg tggcggcttt agttcccaaa aaggaaattg
      361 aaggagatat gggtgatctc caaatgggtt cacaggcccg gcttatgagt caggctttac
      421 gtaaattaac cggcgttatt tctaaatcta aaactattgt tatttttatt aatcagcttc
      481 gaatgaaaat tggtgttatg tttggcaacc ccgagaccac cactggtggt actgccttaa
      541 agttttatgc ttcagtgcgg attgacatca ggaagattgc tcagattaag cagggcgata
      601 aaatgattgg taatcgggta aagtgtaaaa ttgtcaaaaa taaagtagct gctcctttta
      661 agagttgtga gtttgatatt atgtataatg aaggtatttc tattgccggg gatttgttag
      721 atacgggagt tcaaagagga acagttacta aaactggtaa ttcatatagt tttggtgaga
      781 ttaaattagg ggtaggccgg gaaaatgcta aagctttttt gaaggaacat ccagaattaa
      841 tgagccagat tagagaacag attttagccg atattgctgc taagggagaa tagtgatatt
      901 gaaagaatta aaataaaaaa attaaaagat aaaaagaccc ataatattgg gtctttttat
      961 ctaaaagtca ggaaattata caattattta gattcattta ttctttctac atattcttca
     1021 gtgtcagtat tgacgcgcac gatatcgccc tcattaataa agagaggggc attaatttta
     1081 gcgccagttt ctagttcaat ttgtttcata acattaccag ccgtgttgcc ccttactccc
     1141 ggcggggcct gagtgacttt atattctact ttaattggta attctaccgt taccggtcgg
     1201 ccctggaaat atagaacgtc aacttctagg ccgtctttta agaatttttc tttaccccct
     1261 aggtcttcta gattgacagc aaattgctca taggtttcgc tatccataaa attagcaaat
     1321 tgactatcca gatacataaa agtagctttt cttttttcaa catcagcctc ttctgcccgg
     1381 tcattccctt gccaagtttt ttctaaaact gccccagtaa ttagattacg caatttagtt
     1441 ttaagcactg cgccacctcg gcccatctta tgatgatcgg cgcgaataat agtataaggt
     1501 tcaccgttta cattgataac cttaccaggt ttaatttcgt ttaaggttag catatttttt
     1561 attgacgggt aaacattaag agtcccataa ttcttgctcg tttaatagct aaagctagct
     1621 gacgttgatg cttatcacaa gtgccagtac ggcgtttagg taaaattttg cctaaagagt
     1681 tggtatagcg acgtaaaagg aaagtgtctt tgtagtcaat ctctttaatg ttgttgtggc
     1741 aaaaataaca gggcttgcct gaagtgtagt gattcataat tttaattatt aaaatggtat
     1801 atcttcaatc ttaatttcct cttcctccgg gttctctggg atggattcgg tcggtagttc
     1861 gctttcttct ccttccgagg ggggtggtgt aaagggtccg gttgtcccgc cagctcggtc
     1921 gagcataatg atgttatcgg caatgacttc tgtccgataa cgtttagtgt tgtcttgcgc
     1981 tacccagtca cgggtttgta accgtccctc cacgtatact ttagagcctt tgtggagata
     2041 ttggcggcag atatcggcta atcttttcca agctactaca ttatggaatt cagctttttc
     2101 ctggcgctgg ccagtttggt ctgtccagac taaattagta accaccgtaa atgaagcaac
     2161 tggctgcccg ctagccgtac tgcgaatttc ggggtcacgg gtgagattgc cgataagcat
     2221 tactttgttt aagtccatag gcatatttta aaataattaa aaactagctt tagactactg
     2281 ttggttcttc tactgtatta gtggtttgag tctcgtttag attttgttca cttggtgctt
     2341 caggtttact cttttgatta atatctattt ctccagcttt aattttagcc tcgcgcttag
     2401 caatttcctc tttagcggct agaatttctt ccactgtccg gggctggcgt ttaataattt
     2461 ggtgtcttaa aatatcatta gagagtcgca gataacggtt aatttcttca attttgttag
     2521 cggggagatc aaactctagc aggcggtagt agccaaattg aaaaccctta atgggataag
     2581 acatgtgcct tttgccccaa ttttcacggt aaacaatggt gccattgtta ctggtaataa
     2641 ggtcttctac tttttgggca attggctcag tttcttcact ggtaaattta ttagagacaa
     2701 tgtaaagtaa ttcataacgg ggaatttggg agcttttgtt tttagccata cattaaaatt
     2761 aataaataaa aaccctcaac cataggtcta tgagccgagg ggatgctcta ttcttctgcc
     2821 cttgatatct ttctaaagaa gatatcattt aaacctatgg tttaaatctg gaaaaggcaa
     2881 atacaattat tttgtctttt gtaaccgtaa tatagcataa gggtaaataa attgcaaggc
     2941 taaagatttt gtgttagttt agcttatatg aattattttt ttattttggg taatcatcct
     3001 gctttatctt tgtctgaact ttcggtcaaa attgccggta actggcaaat agttaatgat
     3061 gctgtggtga ccctgcatta tgatggtgag cttgcggcta atttattaga gagtttaggg
     3121 ggtacaatta agtttggaaa aataatggga caatttaaaa aattagatga agttgccgaa
     3181 gaagatttgg ctagctattt attatcattg aatcaagggg atggtaagat tcactttggt
     3241 ctttctaact atagttctat tgtcttggat aggtcttttg gtctgcagat aaaacgccgt
     3301 cttcaggttg ccggtcgatc agcgcgttgg gtagttggtc gggataaagt tttatccagt
     3361 gtaattgttg agcagaatca tttacttact aagggcaagg aaatagtctt ttggcgttat
     3421 aatgattatt ggcagtgggg aattactatt ggtgtccagc catttaaggg gttatcggct
     3481 cgtgattatg gccggccagc gcgtgatgac cgttctggga tgttgccccc caaattagcc
     3541 cagataatga ttaatctagc tggcttacgg ccgggcgata ttctccttga tccattttgc
     3601 ggctccggga caattttaca ggaagcggcc ttactgggag ctagtaaagt tatagggata
     3661 gataatgcgc ctcaggctat taaaaatagc caagataatt gggcttggtt tgctcagcgt
     3721 tggcgagtta atacttcggc cgagtttaga ctgggtgaag ctactaaggt ttctcagatt
     3781 ttatcgtcaa aaattaatcg cattgtaact gaaccttatt taggtccatc ccggggaccg
     3841 cgtaatttcc cagctattat taaagagctg gaagtattat atagtcaagc tctacgtgat
     3901 tggcggtcgg ttattactaa agaggggcgg gtagtaatga tttggcctat ttttctttgc
     3961 ggtaccaaaa aatattatgt tcagccagat atttctggct ggcaaactat agagtttagt
     4021 aaattagcta gacaaagctg gccccaggaa tatactccgg gcggtcattt aatttatggc
     4081 cgatctcggc agactgtctg gcgagccata gttggtcttc agccggttta attattgatg
     4141 agaaaattac agacatcacc atcttgaatt aggtaatttt tgccttctaa acggatgagg
     4201 cctaattctt tagccttagt ttcactgccg gctcggagta atgtttccca gttaataact
     4261 tcagctctaa tgaagttttt cataaagtcg gtatgaataa ccccggcagc ctggggtgct
     4321 ggggtattac gtttaaccgt ccaggccctc gtttcatcac ggccggtagt taaaaaagta
     4381 attagtccta agaggttata ggcttgagca atcaatttat tcagtccgct gtcttgtagg
     4441 cctagctctt taatatagtc agcttgttct tcttcgggca gactggcaat ttctgcctct
     4501 aatttagcat ttaagacaat agctggtcca tctaaatgga gcagtaaatt agccggttct
     4561 tcactattta taacataaat aactggtttg ctggttaata aattaagttc cttagctaat
     4621 tcttgctcat cggccgtaag agagcatcgt cttaagcttt gtccttcatt taaatagtcc
     4681 cttatttttt ttagagtatc tacttgctta ctaataagtt tatcgccact tttagcttct
     4741 ttacttattt tatctaaccg tctattaaca gtagctaaat cagcatagat taattctaag
     4801 tcaataattt ctttgtctag ggaggggtta atgcgaccct ccacatggac tacgttacta
     4861 tcctggaaat tacgaatgac gtggcaaatg gcatcaacat tgcggatatt agcaagaaat
     4921 tgattgccca acccctctcc ttcatgagct cctttaacta ggccagcaat gtcgacgaat
     4981 tcaatagtgg cataaatttg cttggctgaa ttggacatag cagctagttt agttacccgt
     5041 tcatctggta ctttaactac cccgacatta ggttcaatag tgcagaaagg ataattggca
     5101 gcatcaacct gctttttagt cagggcgttg aaaagtgtgg atttgccaac gttaggtagg
     5161 ccaataatac caagagataa agtcataaga ttcattaaaa atttagcaga ttttagaata
     5221 aataacaatt attgacgagt agggagtgat gtgttaagct tgtcagtgct tattagcttc
     5281 atttttagga gtcgtagttc agttggttag aacgctgccc tgtcacggca gaggtcacgg
     5341 gttcgaggcc cgtcggctcc gcaagacacc c
//
LOCUS       DAOOIS010000024         4992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_13367_length_4992_cov_63.067855, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000024 DAOOIS010000000
VERSION     DAOOIS010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13367_length_4992_cov_63.067855"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1936)
                     /gene="ppsA"
                     /locus_tag="PK960_02490"
     CDS             complement(<1..>1936)
                     /gene="ppsA"
                     /locus_tag="PK960_02490"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HPD08067.1"
                     /translation="FYKTKNLSVAVRSSATAEDLPDASFAGQQETYLNVSGEANVLMA
                     VKKCIASLFTDRAISYRVDKNFDHFSIALSVGIQKMIRSDLGSSGIMFTIDTETGFSN
                     VVIIDSIYGLGENIVQGRVNPDEFYVFKPTLAIIDKTLGEKKIKMVYDLKTKATSRVK
                     NIPVPKKDQFKFSISDDQVKTLARWGMAIEQHYKKPMDMEWALDGQDGQLYMVQARPE
                     TVNSQRDYNILEEYTIKKRGKVLVEGRSVGSRVGAGIVNRIINVKDINKFKPGQILVT
                     AMTDPDWEPIMKMAAAIITDKGGRTCHAAIVSRELGIPCIVGTGNATKKLSTGQTVTV
                     SCAEGDVGFVYEGKIPFSIKKTNVQKIPRPDCKIMLNVGSPDIAFANSFLPNDGVGLA
                     REEFIVNDYIRIHPLALVNYHKIKDPQLKKQIDELTIGYSDKKQFFIDKLAQGVGTIA
                     AAFYPKDVIVRLSDFKTNEYANLIGGQDYEPVESNPMIGWRGASRYYDPKFEPAFALE
                     CLALKKVREEFGLKNLKIMVPFCRTIEEGKLVIKTMAKYGLKQGRDGLEIYVMAEIPT
                     NILLANDFAKVFDGFSIGSNDLTQLTLGIDRDAGNLQIAGQSNENNPSVKSLIQFLIE
                     VGHRTHTKVGICGQAPSDFP"
     assembly_gap    1937..2036
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2296..2377
                     /locus_tag="PK960_02495"
     tRNA            2296..2377
                     /locus_tag="PK960_02495"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2330..2332,aa:Leu,seq:tag)
     gene            2386..>3359
                     /locus_tag="PK960_02500"
     CDS             2386..>3359
                     /locus_tag="PK960_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08068.1"
                     /translation="MLNFEETILQTIKQAHHVLVAADIKSTPSNGAAATALVSGLRQA
                     GIQADFVANASLDASWSVLGSIIQPNLELPSEDFIISLNLSRTKVGQVKYKIEGDKLK
                     FFVKPNGGQFTEEDVELIFGRPPYDLIITIGVAAPEVLGEIYNQNSGLFYQTPIINID
                     CQVDNENYGQVNLVDLVASSTAEIVYDLFRALDWHIDADVATYLLAGLIYETKNFTNT
                     KATPQVLVAAANLIKEGARREAIVNEWYASVELSTLHLWGKILLNLEQTEDGLVWSKL
                     DSNEVVPSVSLIRQAIERLLIGLQDAKVIAVFYNLASAQPTVSLSLLK"
     assembly_gap    3360..3459
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3460..4595)
                     /locus_tag="PK960_02505"
     CDS             complement(<3460..4595)
                     /locus_tag="PK960_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPD08069.1"
                     /translation="MTIKEGFRLILGRKKILLIWLITSNYCLPFYVQAQTLIFHELLP
                     RPATGEKEYLEIINVSQENVSSAGWHIADEAKHDNPSPLPDYILGPGQTWHLETKTLG
                     ISLNNDAESLYLWQPDGTLVDSLSYTKAPSGAIYRLEGDGWKWLDTTVSLNSTTPATS
                     ETITSPISKATSTLDKVQGIVSALPYQLSSQYFYITSPDNTGGLKIYCYYKLWPQLQL
                     GDLIEVSGTLVSTTQETKLNIKGPQDIKVISSNYQLKIKEITTSDINGLLPGQLVAIK
                     GKVAAKNQSTIYLTDDFGEVLIQLKTGTELKNSNFTVGEKYRIIGLIIDNNGRRLVPR
                     QLSDVQKIIQPTTTSATTTLDLSTSTTTKTISPFTWLGGLLIAGV"
     gene            complement(4612..>4992)
                     /locus_tag="PK960_02510"
     CDS             complement(4612..>4992)
                     /locus_tag="PK960_02510"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPD08070.1"
                     /translation="GNLHILRNQLNETAKNLANYLTVPELNHYAMEGLSFPPNLPDTI
                     IILSFKVKGLPQRLERRLYLTDKIFKQNKLPVLTYEAKSSSHLGAALEILAFGSWLSY
                     YLGLLNGVDPAKIPWVDLFKKELA"
BASE COUNT         1406 a          868 c          989 g         1529 t
ORIGIN      
        1 tctgggaagt cgcttggtgc ttgaccgcag ataccaactt tagtatgagt tcggtgacca
       61 acttcaatta aaaattgaat cagagattta actgaagggt tattttcatt agattgtcca
      121 gcgatttgta aattgccagc atcacggtca atgcccaatg ttaactgagt taggtcatta
      181 gaaccaatag aaaagccatc aaaaacttta gcaaaatcgt tagctagtaa aatattagtg
      241 ggaatttcgg ccataacata gatttccagt ccatcacgtc cttgcttaag gccatattta
      301 gccatagttt taatgactaa tttaccttct tcaatggtgc ggcaaaaggg gaccataatc
      361 tttaaatttt tgagaccgaa ttcttccctg actttcttta gagctagaca ctctagagcg
      421 aaggctggtt caaatttagg atcataatag cggctagcgc cacgccagcc gatcatggga
      481 ttagattcta ctggttcata atcttggccg ccaattaagt tagcatattc gttagtttta
      541 aaatcggaaa gacggacaat aacatcttta ggataaaagg cggcggcaat agtgcctaca
      601 ccttgagcta atttgtcaat aaagaattgt tttttgtctg aatagccaat agttaattca
      661 tctatttgtt ttttaagctg aggatcctta attttatgat aattaactag agctaggggg
      721 tggatacgga tatagtcatt aacaataaac tcttctctag ctagtccgac accatcattg
      781 ggcaggaagg aattagcaaa agcaatgtct ggcgaaccga cattaagcat aattttgcaa
      841 tctggtcgag gtattttttg aacattggtc tttttgatag aaaaggggat tttaccttca
      901 tagacaaaac cgacatcacc ttcagcacaa ctcacggtta cggtttggcc agtagataat
      961 tttttggtag cattgcccgt gcctactata caaggaatgc ctaactctct agacacaata
     1021 gcagcatgac aagtgcggcc acctttatcg gttatgatgg cggcagccat tttcataatt
     1081 ggctcccagt caggatcagt catagcggtt acgaggattt gcccgggctt aaatttatta
     1141 atatctttaa cattaataat ccgattaact attcctgccc cgacacggct ccccacactg
     1201 cggccctcca ctaaaacttt acctcttttt ttaatggtat attcttccag aatattataa
     1261 tcacgctggc tattaacagt ctcagggcgg gcttgtacca tatatagttg gccgtcttga
     1321 ccgtctaggg cccactccat atccattggt tttttataat gttgttctat agccatgccc
     1381 cagcgtgcca gagtttttac ttggtcatcg gaaatggaaa atttaaattg gtcttttttg
     1441 ggtaccggga tatttttaac tcgggaagtg gctttggttt ttaagtcata aaccattttt
     1501 atttttttct ctcccagggt tttatcaata atggcgaggg ttggtttaaa aacataaaat
     1561 tcatcaggat tgacccggcc ctggacaata ttttctccca agccataaat agaatcaata
     1621 attactacat tagaaaaacc agtttccgtg tcaatagtaa acattatgcc tgacgagccc
     1681 aggtcactgc ggatcatttt ttgaattccg acagacaagg caatagaaaa atggtcaaaa
     1741 tttttatcaa cgcggtaaga aatagcccgg tcagtaaata gtgaggcaat acatttttta
     1801 acagccataa ggacattggc ttctccactg acatttaaat aggtttcttg ctggccagca
     1861 aaagaagcat cgggtaaatc ttcggcagta gctgaagaac ggacagccac gcttaaattt
     1921 tttgttttat aaaaacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaccc
     2041 cagatttagt taatttttga tacatttctc cgagtgacgc atttttgcca cccacaatag
     2101 ggacatcttt aatggttatt tcattaaaga atttaacata ttggctcatg tttttgttgg
     2161 cgcctcgtct aaattgagga ggctaattat aataattaat attgttagtg ataactattg
     2221 tgaaattata gcatggaata gcagtcttga caaaagacta tttttacctg acaatagctt
     2281 attagctaaa tttaggcagg cgtggtgaaa ttggtagaca cgcatgcttt aggagcatgt
     2341 ggagcaatcc atgtgggttc aagtcccgcc gcctgcactg agactatgtt aaattttgaa
     2401 gaaacaatct tacagaccat caagcaagcc catcatgttt tagtagctgc tgatattaaa
     2461 agtactccta gcaatggtgc ggcagctaca gccctagtta gtgggttgag gcaggctggt
     2521 attcaggctg actttgtagc taatgcctca cttgatgcta gttggtcggt attgggatca
     2581 attattcagc ctaacctgga attacccagc gaggatttta ttattagctt aaacttaagt
     2641 cgcactaaag ttggccaggt aaaatataaa attgaaggtg ataaattaaa attttttgtt
     2701 aagcccaatg gtggtcaatt caccgaagaa gatgtagagt taatttttgg ccgtccgcct
     2761 tatgatttga taattaccat aggggtggcc gctcctgaag tactgggtga aatatataat
     2821 caaaattctg gtttatttta tcagacacca ataattaaca tagattgtca ggtagataat
     2881 gaaaattacg gtcaagttaa tttggtagac ttagtggcta gttcgacggc tgagattgtt
     2941 tatgatttat ttcgagctct agattggcac atagatgctg atgtggctac ttatttatta
     3001 gcagggttaa tttatgaaac taaaaatttt actaatacta aggcaacacc ccaagtatta
     3061 gtagcagctg cgaatttaat taaagaaggg gcacgacgtg aggctattgt taatgagtgg
     3121 tatgcttctg ttgaactctc aaccttgcat ttgtggggta aaattttatt aaatctggaa
     3181 caaaccgaag atggtttagt ttggtctaaa ttggatagta atgaggttgt accttctgtg
     3241 tctttaatcc gtcaggctat tgagcgattg ttaattggtc tgcaagatgc taaagtaata
     3301 gcagtttttt ataacctggc ctcagctcag ccaactgttt ctctgtcact gctaaagaan
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ccccagcaat taataaaccc
     3481 cctagccaag taaatggcga gatagtcttg gtggtagttg aagtacttaa atctagggta
     3541 gtagtagctg aagtagtagt tggctgaata attttttgga cgtcacttaa ttgacgaggc
     3601 acgagacgtc ggccattatt gtctatgatt aaaccgatta ttctatattt ttctcccacg
     3661 gtaaaattac tattttttag ttccgtacca gtttttaatt gtattaacac ttcgccaaaa
     3721 tcatcagtta aataaatagt cgactgattt ttagccgcta cttttccttt aatagccact
     3781 aattgtccag gcagtaaacc attaatatct gaagtagtga tttctttaat ctttagttga
     3841 tagttgctag atattacttt aatgtcttgt gggcccttaa tatttaattt agtttcttgg
     3901 gtggttgaga ccagagtgcc acttacttca attaaatcac caagctgaag ttggggccat
     3961 agtttataat agcagtaaat ttttagtccg cccgtattgt ctggagaagt aatataaaaa
     4021 tattggctag aaagttggta aggtaaggca ctaactatgc cctgtacttt atctaaagtg
     4081 cttgttgctt tactaattgg agaggtaata gtttcggacg ttgccggagt ggtggagttt
     4141 agactaacag tggtatctag ccacttccag ccatctccct caaggcggta aatggcacca
     4201 ctaggagcct tggtatagct tagagaatcc actagagtgc catcaggttg ccaaagatat
     4261 aaagattcgg cgtcgttatt aagacttata cccagtgttt tagtttccag atgccaagtt
     4321 tgacctgggc caagaatata gtctggtagg gggctaggat tgtcgtgttt agcttcgtcg
     4381 gcaatatgcc agccagccga gcttacattt tcttggctta cattaataat ttccaggtat
     4441 tctttttcac ccgtagctgg tcggggtaag agctcatgaa atattaaagt ttgcgcctga
     4501 acataaaagg gcaggcaata attactggtg atgagccaga ttaataagat tttttttcgg
     4561 ccaagaatta gccgaaaccc ctccttaata gtcatatatt taagttaaaa attaagctag
     4621 ttctttttta aatagatcca cccagggtat tttagctgga tctactccat taagtaggcc
     4681 aagataatag cttagccagc tgccaaaagc taggatctct aaagctgcac ccagatggct
     4741 agaagactta gcctcataag ttagtactgg taatttattt tgtttaaaga ttttatcagt
     4801 aagataaagc cgtcgttcta aacgctgagg taatccttta actttaaaag agaggataat
     4861 tatggtgtcg ggtaagttgg gaggaaagga tagcccttcc atagcatagt ggtttaattc
     4921 cggaacagtt aaataattcg ctagattttt ggctgtttca tttaattggt tgcgtaaaat
     4981 atgaagattg cc
//
LOCUS       DAOOIS010000025         4601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_14740_length_4601_cov_41.300484, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000025 DAOOIS010000000
VERSION     DAOOIS010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14740_length_4601_cov_41.300484"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..660)
                     /gene="infC"
                     /locus_tag="PK960_02515"
     CDS             complement(106..660)
                     /gene="infC"
                     /locus_tag="PK960_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPD08071.1"
                     /translation="MRRTFRKPKPKEEKKFRANEQIIAREVFVIDENGNSLGLMATET
                     AIKLAQDAELDLVEVNPNNNPPVAKIVDYGQFRYRIEKKMQQQKSRQKKIDLKEVRLS
                     IRISEHDLKMRVDQGYKFLSKGDKLKISVVLRGRERQHPEKAEETINRYLKQLETLAD
                     QTLSIEEPLTKLNDRFSMVVANKK"
     gene            complement(688..759)
                     /locus_tag="PK960_02520"
     tRNA            complement(688..759)
                     /locus_tag="PK960_02520"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(724..726),aa:Gly,seq:gcc)
     gene            complement(775..860)
                     /locus_tag="PK960_02525"
     tRNA            complement(775..860)
                     /locus_tag="PK960_02525"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(824..826),aa:Leu,seq:taa)
     gene            complement(866..1243)
                     /gene="ssrA"
                     /locus_tag="PK960_02530"
     tmRNA           complement(866..1243)
                     /gene="ssrA"
                     /locus_tag="PK960_02530"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1236..1697)
                     /gene="smpB"
                     /locus_tag="PK960_02535"
     CDS             complement(1236..1697)
                     /gene="smpB"
                     /locus_tag="PK960_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPD08072.1"
                     /translation="MSTLAVNKRANYDYEILENYEAGLVLTGQEVKAVRAGQINLKGS
                     YVVFHGGDHPQAFLINAHIGHYKPAGPLANYDPTRSRRLLLKRKELNHLFGRTTNEGL
                     TVIPLKLYTKGSLLKLEIALGRGKKKYDKRQAIQKREWDRRRRTLTKQIRG"
     gene            complement(1708..3075)
                     /gene="tig"
                     /locus_tag="PK960_02540"
     CDS             complement(1708..3075)
                     /gene="tig"
                     /locus_tag="PK960_02540"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPD08073.1"
                     /translation="MEINSQKNDGQLEITVKLSNEEFKPYILEGARRIANEVKLPGFR
                     PGKAPYAVIKAKVGEMTILEEAARVAINHTIDEAIKRGATDQATIGQPNVDITKLAPD
                     NPLEYKIKVNLLPKVTIKDYTNLAIKRPEITIPEEQINKTLEQLREANAQKVASLEPA
                     KMGDLVIANIQISVDKVPIEGGQGQNVNIILGKDYLVPGFDQQLLGAVVGQELQFQIP
                     YPKDHYQKALAGKLVDFKVKINEIWHRQLPKLDDQLAKNFGFENLAALKENIKDSLML
                     EKQRQLDNEMEIKIFEELINRASIEKIPESLINKEAEAMLDELQYNLEQNGGNLNDYL
                     SSLKKTKEQFILEILPQAVKRVKTALIIKEITRLEGITTNREEVDKELDNLKEHYKDN
                     PEIMSRLNMPDYREYLANVIVSNKTVRFLKDHNLVTDNKEKTAPKTTKKPDIEKPKKT
                     TRSKK"
     gene            3416..>4601
                     /locus_tag="PK960_02545"
     CDS             3416..>4601
                     /locus_tag="PK960_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08074.1"
                     /translation="MDDLAKLHAVYLNYYQLMQRRRTAAKKHDHFLSLIFSIALSVLV
                     VIFSAQLLSSWRFKADAKDLVGQLLMPTALAADLSATVEPYTTNLVMNAGQEATINLT
                     FVNNGQTSWPAGQVSLETGPFLKTFSKVEHKSWQSFFKVTTLTKEVKPKEKINISFKI
                     AGPEASIVGVIQENFQLVYNQQPIKGSLARVFIDANQAKPTFVQSSNNITIINTKKPT
                     LTTTTQVATPSKTVEYITGYNKLGEKVQVVKGQYVPGISLTRPSDAAIAAERARLAAI
                     KSSSTSDQAFCIALSSSERANEPQCQTSDSEMQADSGIVEYSRKLTEEPLIRVGLFSS
                     DQVQRITSDQPFDIYAANNLLVGSVPANTVVLVYYVRSTKQYGVIVNDNVYQTLNYLR
                     FVS"
BASE COUNT         1269 a          834 c          850 g         1648 t
ORIGIN      
        1 agtaagtttc atccgcttag caactggctg ataagtcttt aatttcggca tatttagaat
       61 aataaaaaaa ggttttttac acctaatttc ataagaaata attggctatt ttttattagc
      121 cactaccata ctaaagcgat cattgagttt tgtcaagggt tcttcgatag atagagtttg
      181 gtccgctagt gtttctaatt gttttaaata gcggttaata gtctcttcgg ctttttctgg
      241 atgctgtctt tctctacccc ttaaaacaac cgatattttt aatttatccc cttttgatag
      301 aaatttatag ccttggtcaa ctcgcatttt taaatcatgc tcactaatcc taatagatag
      361 tcttacttct ttaaggtcaa tttttttctg gcgggatttt tgttgctgca ttttcttctc
      421 aatacggtag cggaattggc cataatctac aatttttgct acaggtggat tgttgttggg
      481 attgacttct actaaatcta gttcggcatc ttgggccaat ttaatagcag tctcagtggc
      541 cattaggcct aaagaattac cattttcgtc aattacaaaa acctcccggg caataatttg
      601 ttcattggct cggaattttt tttcttcttt tggtttaggt ttgcgaaaag ttcgtctcat
      661 ggaaattgtt tttgagcatt aattatgagc gggtaacggg aatcgaaccc gtatctctac
      721 catggcaagg taatgtaata gccattatac gatacccgct taaataagcc agcgtgccgc
      781 gagagggact cgaaccctca tgatgtttta atatcgtcgg attttaagtc cgatgcgtct
      841 accaatttcg ccatcgcggc attttatgtg gagatgaggg gagttgaacc cctgtatcat
      901 aagaggcaat caatcgtttt tacgaataat agtccaattt tctatacctt aaaaatataa
      961 attggacaaa aattttttaa ggagcctgat tataatcttg acccaatttc aaacaggcgg
     1021 attgatttgg ggtcatagcc taaaaaaatg acaccgatag ttgattatta ggcatggtca
     1081 acatcgatgg ccgctgtatt aagcagcgac aggagcaaaa gcgaattgag attttacttt
     1141 tgctaggatg tttttggcat ctattggttt tgatgtttag gtgtaatcct cacctattcg
     1201 ctcgattgat taccatatct tatgagcgaa gcccgtcatc ccctaatttg ttttgttaaa
     1261 gttcggcgtc ttctatccca ctcacgtttt tggattgctt gacgtttatc atacttcttt
     1321 ttgccccgtc ctaaagcaat ctctaatttc agtaaactgc ccttagtata tagtttgagc
     1381 ggtatgactg tcaagccctc gttagtagtt cgtccaaaaa gatgatttag ttctttacgc
     1441 tttaataata gccgtcggct acgggtgggg tcataattag ctaaaggtcc agctggttta
     1501 taatgaccaa tgtgggcatt aattaaaaaa gcttgaggat ggtcaccgcc atgaaagacg
     1561 acataactgc cttttaggtt tatttgtcca gctcggactg cttttacttc ctggccagtc
     1621 aaaactagac ctgcttcata attttccagg atttcatagt cataattagc tcgtttgttg
     1681 actgctagag tggacattgg cgtaagatta ttttttggag cgggttgtct ttttgggttt
     1741 ttctatgtct ggctttttag ttgttttggg agccgttttt tctttattgt cagtaacgag
     1801 attgtggtct tttaagaaac gtacagtttt attactaaca atgacattgg ccaaatattc
     1861 tcggtagtca ggcatattga gacggctcat tatttctggg ttatccttat aatgttcctt
     1921 tagattgtcc aattctttat ctacttcctc cctattagta gttattcctt ctagtctggt
     1981 aatttcttta ataatgaggg cagttttaac tcgcttaact gcttggggca gtatctctaa
     2041 aataaattgt tccttagttt tttttagact gctcaagtag tcatttaaat taccaccatt
     2101 ttgttctaga ttatattgta attcatctaa cattgcttcg gcttctttat taattaatga
     2161 ttcaggaatt ttttcaattg aggctcgatt tattaattct tcaaatattt ttatttccat
     2221 ttcattgtca agttgccgct gtttttctag catcagacta tctttaatat tctcttttag
     2281 cgccgctaag ttttcaaagc caaaattctt agcaagctgg tcatccaact ttggtaattg
     2341 acgatgccaa atctcattaa ttttaacttt gaaatccact aatttgccgg ctaaagcttt
     2401 ttggtagtgg tctttgggat aggggatttg gaattgtaat tcttgcccta ctacagcgcc
     2461 taataattgt tgatcaaaac caggtactag ataatctttt cctagaatta tattaacatt
     2521 ctgtccttgg ccaccttcta ttggtacctt gtctaccgag atttgaatat tggctattac
     2581 taaatcaccc attttagctg gttccagact cgctaccttt tgagcattag cttcacgtaa
     2641 ttgttctagg gttttgttta tttgttcctc tggtattgtg atctctggcc gtttaatggc
     2701 taaattggta tagtctttaa ttgttacttt aggcagtaaa ttgactttaa ttttatattc
     2761 taaaggatta tccggggcta gtttagtaat atctacattg ggttgcccaa tggttgcttg
     2821 gtcagtggct ccacgtttga tagcctcgtc aatggtgtgg ttgatagcta ctctggctgc
     2881 ttcttctaat atagtcattt cgccaacctt agctttaatg acggcatagg gagctttacc
     2941 aggtctaaaa cctggcaact tgacttcatt agcaatacgt cttgcacctt ctaaaatata
     3001 gggtttaaat tcttcattag acaatttgac agtaatttct agttgtccat cgtttttttg
     3061 ggagttaatt tccatagtat ttttattttt aaattaatag atgaatattg gaagattagt
     3121 ctaaaataat gtagactaat cttaataaat tcctctcata taattttaat attttatatt
     3181 gttatgctaa tgatctaaaa agatcttatt tattatataa agttcacttt ttattgttat
     3241 tactcttaaa aactatttag acatttcata gtttaacaaa catctagccg attggcaact
     3301 tttaagaaga taagtctaca tttatcttga cttttctcta tttttttgct atacttaagt
     3361 ttagtcgaac cattccaaat ttataaacag aagtaataaa ttaaaattaa aatatatgga
     3421 tgacttagcc aaactacacg ccgtttacct taattattac caattgatgc aacgtagaag
     3481 gacagcggcc aaaaaacatg accattttct aagtctaatt tttagtatag ccttgtctgt
     3541 tttagtagtt attttttcgg cccaacttct aagcagttgg cgttttaagg ctgatgctaa
     3601 agatttggtc gggcaattat tgatgccaac tgccctggct gccgatttaa gcgctactgt
     3661 tgagccatat accactaact tagttatgaa tgccgggcaa gaagccacta ttaatcttac
     3721 tttcgttaat aatggccaaa ctagctggcc ggctggccag gttagtctag aaaccggccc
     3781 ttttctaaaa actttttcta aagtagaaca caaaagttgg cagtcttttt ttaaagtaac
     3841 tactctaact aaagaagtaa agcctaagga gaaaataaac ataagcttta agattgctgg
     3901 gccagaagcc agcatcgtcg gtgttattca ggagaatttt caattagtgt ataatcagca
     3961 accaattaaa gggagcctag ctcgggtttt tattgatgct aatcaagcta aacctacctt
     4021 tgtccaaagt tctaataata taaccattat taatactaaa aagccgacct taacaacaac
     4081 tactcaagta gcaaccccaa gcaagacagt agagtatatt accgggtata ataagctggg
     4141 tgaaaaagtg caggtggtta aaggacagta tgtgccaggc atttctctaa ctagaccatc
     4201 tgatgctgcc atagccgctg aacgagcccg actggcggct ataaaatcta gctctactag
     4261 cgatcaggcg ttttgtattg ctctctcatc ttctgaaaga gccaatgagc cccagtgtca
     4321 aactagtgat agcgaaatgc aagctgattc tgggatagtg gaatattctc gtaaattaac
     4381 cgaggagcca ttaattaggg tgggtttatt ttcgagtgat caggtgcaga gaattaccag
     4441 tgatcagccc tttgatatct atgctgctaa taatttatta gttggctcag taccggctaa
     4501 tactgtagta ctagtttatt atgttcgaag caccaagcag tatggtgtta tagtcaacga
     4561 taatgtttat cagactctta attatttacg ttttgttagt c
//
LOCUS       DAOOIS010000026         4416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_15479_length_4416_cov_34.470764, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000026 DAOOIS010000000
VERSION     DAOOIS010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15479_length_4416_cov_34.470764"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1745
                     /locus_tag="PK960_02550"
     CDS             174..1745
                     /locus_tag="PK960_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08075.1"
                     /translation="MHLSPRLICRWMMIASVMAVVIIFAPRPTRAAEFNPNLIISDAE
                     LLDSSTMSLADIQQFLEMHGSYLANYRGEDYYGTVRSAAEIIYNAATNSYDCSGIDIN
                     NPKDIEEAKAKCVKVSINPRFLLVLLQKEQSLIEDPSPKASQLDWAMGYGCPDGAACS
                     ERWRGFGKQVNAAALQFYDYMMNPRYYHYQVGQSYTIFNTNRDPVVVTPANRATAALY
                     NYTPHVYNGNYNLWKLWQKYFTKIYPDGSLLQPEGEEVVYLIQSGHKRPFASRGALVS
                     RFDPRKIVTVKPADLENYPLGAPIKFQQYSLVQAPSGAIYLIVDDKRRGFVSKQAFSK
                     MGFNMQEVVKASWEDLAPYKESAPITVTSTYPTGALLQDKKTGGVYWVEEGFKYPLVD
                     RVLLSTKFANRRIIPADATELAAYQTGAPVKLDDGNLIKIEGSPNVYILDHGEKRLIP
                     SERLFEGLGYKWSNIITVNPQHFELYPFGQIVEDRLPAEVGPPDATSTPTSTLPDLVA
                     TSTANISSTPNSLSP"
     gene            1747..2541
                     /locus_tag="PK960_02555"
     CDS             1747..2541
                     /locus_tag="PK960_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08076.1"
                     /translation="MALVFAALVPHSPLLVPNIGREHRQLLAKTLKAFEELSTALAEA
                     QPEAIIIMSPHIENGANWWNANVSLTYHVDLSIFGDLLTKDSWEGALRLVIDLQDYLW
                     PDQSLRLYSQPALDYGTSIPLLLLGSAVQRVKILPVGYINNQPERQALFGHQLGEFSR
                     QWPGRIALLGSGDLAHTLGPDQPSKAQGQGHKFDQELQRALEQNDEAAILNMPSDLLS
                     KTGQCAWLGYLTVNAALANQLGQWHNLVYEAPFGVGSAIWYWQTSS"
     gene            complement(2550..2828)
                     /locus_tag="PK960_02560"
     CDS             complement(2550..2828)
                     /locus_tag="PK960_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08077.1"
                     /translation="MTRDVRQFTTIGDLIKQPRKTIKPPAYPWQQLALEVIERLRVPN
                     FKRSAIFKVCQQFPESTIRQALIDTQELCEGGNRWQYFFKILSNYHKK"
     gene            2865..3338
                     /gene="def"
                     /locus_tag="PK960_02565"
     CDS             2865..3338
                     /gene="def"
                     /locus_tag="PK960_02565"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPD08078.1"
                     /translation="MDQKQIDKGHVRRLVIYPDSRLRRTAQVVDLARLQDKKFQELIV
                     DMAKTMLIKDGIGLAAPQVGRNWRLIVVNTDEGVWPMINPIITKLSSSTTIEEEGCLS
                     LPNTFGQVRRAKSLQCHYINELGKECNLTAKGTLARVIQHEIDHLNGILFIDYLK"
     gene            3344..4288
                     /gene="fmt"
                     /locus_tag="PK960_02570"
     CDS             3344..4288
                     /gene="fmt"
                     /locus_tag="PK960_02570"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPD08079.1"
                     /translation="MTIMPPKKISLVLAGSPQLAVPAFEALWRDGRYNIVGVLTQPDK
                     PAGRGQKITPPPVKVWAQSHNLPVWQPEYLPSWLTIAQEIKPEMIVVMAYGQFIPSSL
                     LNLPWLGWINVHGSLLPKYRGAGVLTAPILNGDRETGISLIRLTKKLDAGPIIARRVI
                     PLSPIETSGSLAAKIANEAALMLPDVLYQYAQGLLVPKEQDESQATYVGLIKPQDARI
                     DWRKSAVEIERFIRAMQPKPGAWTMWDGQRLKIIASEVADSAGFRPGQVEHISNHLVV
                     GTGQGNLIVDKLQLAGRSVLSGEEFLRGYPNVVSAVWS"
     gene            4339..4410
                     /locus_tag="PK960_02575"
     tRNA            4339..4410
                     /locus_tag="PK960_02575"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4372..4374,aa:Glu,seq:ctc)
BASE COUNT         1269 a          827 c          988 g         1332 t
ORIGIN      
        1 ttcaaaacca gcttctagat gaattggcct tgcaaataat tgaaggtaaa attaaagaag
       61 gagatacagt tattagtgat atcgataaca ataaagtcgt tttccgatta gaagagaaag
      121 ttccaaaaag ttccaactaa aattagacat taatttattg gagtaataat cccatgcatt
      181 tatctccccg cttaatttgt agatggatga tgatagcctc agtaatggct gtggttatta
      241 tttttgcccc acggccaacg cgggcagctg aatttaatcc taatttaatt attagtgatg
      301 ccgaacttct ggactctagc actatgagtc ttgcggacat tcagcaattt ttagagatgc
      361 atggtagtta tttagctaat tatcggggag aagattatta tggcacagtt cgtagtgcag
      421 ccgaaattat ctataatgcc gctactaata gctatgactg tagtgggatt gatattaata
      481 atcctaaaga catagaagag gctaaggcta agtgtgttaa ggtatccatt aatccccgtt
      541 ttcttttagt ccttctacag aaagagcaaa gcttaattga ggatcccagc cctaaagcta
      601 gtcagttaga ctgggcaatg ggttatggct gtccagacgg tgcggcttgt agtgagcgct
      661 ggcgtggttt tggcaagcaa gttaatgccg cggctttaca gttttatgat tatatgatga
      721 atcctcgtta ttatcattat caggtaggac aaagttatac tatatttaat actaatagag
      781 atccggtggt ggttacaccg gctaatcgag ccacggctgc tttatataac tatacccccc
      841 atgtgtataa tggcaactat aatttgtgga aactatggca gaaatatttt accaaaattt
      901 atcctgatgg ttccttatta caacccgagg gcgaagaggt agtatattta attcagtcag
      961 gccataaacg gccgtttgct agtcgtggtg ctttagtctc taggtttgat ccacgtaaaa
     1021 ttgtgactgt gaagccggcg gatttagaaa attaccccct aggggcacca attaagttcc
     1081 agcaatatag tttagtgcaa gctccgtctg gggccattta tctcatagtt gacgataaac
     1141 ggcgaggctt tgtaagtaag caggcattct ccaagatggg ctttaatatg caagaagtcg
     1201 tcaaggccag ttgggaagat ttagcccctt ataaagaatc ggcgcctatt actgtaacct
     1261 ctacttatcc aaccggcgct ttgctccagg ataagaaaac tggcggagtc tattgggtag
     1321 aagagggctt taaatatcca ctggtagatc gggtactgct tagtactaag ttcgccaatc
     1381 gccgaattat acccgccgat gctactgaac tagcagctta ccaaactggg gcgccagtta
     1441 aattagatga tggtaatcta attaaaatag aaggcagtcc taacgtctat attttagacc
     1501 atggcgagaa acgtttaatt cctagtgaac gtttatttga aggtctaggt tataaatgga
     1561 gcaatataat tacggttaat ccccaacatt ttgagcttta tccttttggt cagatagtag
     1621 aagataggtt gccggctgag gttgggccac ctgatgcgac ctccacccca actagcactt
     1681 tgccagattt agtagctact agtactgcta atattagctc cacccctaat tctcttagtc
     1741 cgtaatatgg ccttagtatt tgctgcttta gtaccacatt caccgttact agttcctaat
     1801 atcgggcggg agcatcgtca attgttggca aaaaccttaa aagcttttga ggaattatct
     1861 actgcattag ccgaggctca accagaagct ataattatta tgtcgcccca catagaaaat
     1921 ggtgccaatt ggtggaacgc taatgtttcc ttaacttatc atgttgattt aagcattttt
     1981 ggtgatttac taactaagga tagttgggag ggtgctctta gattagttat agacctgcaa
     2041 gactatttat ggcctgacca gtcccttcgt ctttatagcc agccagcctt agattatggt
     2101 acatctatac ctttattatt attgggttca gctgttcaga gggttaaaat tttaccagtg
     2161 ggttatatta ataatcaacc cgaacgtcaa gccctgtttg gccatcaact aggtgaattc
     2221 tctcgtcaat ggccaggccg gatagctctt ttaggctcag gtgatttagc tcatacctta
     2281 gggcctgacc agccaagcaa agctcaggga cagggtcata aatttgacca ggaacttcag
     2341 cgagccctgg agcaaaatga cgaagcggcg atattaaata tgccctcaga cttattaagt
     2401 aaaacaggcc aatgtgcttg gctgggttat ttaactgtta atgcggcctt agctaatcaa
     2461 ttaggccaat ggcataattt agtttatgaa gccccttttg gagtgggtag cgctatttgg
     2521 tattggcaga cttctagcta aattaatgtt tatttcttgt gataattact aagtattttg
     2581 aaaaaatatt gccaacgatt acctccttcg cagagttctt gggtatcaat gagagcttgg
     2641 cgaatagtag attccggaaa ttgttgacag actttaaaaa tagccgaacg cttaaagttt
     2701 ggcactctaa gtcgttcaat cacttctaga gctaattgtt gccacgggta agctggtggc
     2761 ttaatagttt ttcgtggttg tttaattaag tctccaatag tggtaaattg acggacatca
     2821 cgagtcataa atttaaacta ataatattaa agtataatca agttatggat caaaagcaaa
     2881 ttgacaaggg acatgtccgc cgtttagtca tttatcccga ttctcgatta cggcggacag
     2941 ctcaggtagt agatctagca agactacagg ataaaaaatt ccaagaatta attgtagata
     3001 tggcaaagac catgcttatt aaagatggca ttggtttagc agcaccccaa gtggggcgta
     3061 attggcgcct tattgtcgtt aacactgacg agggcgtatg gccgatgatt aatccgatta
     3121 ttactaaatt aagttctagc actaccatag aagaagaagg atgtctttcc ttgcccaata
     3181 cttttggcca agttagacga gctaaatcgc tccagtgtca ttatattaat gaattaggca
     3241 aggaatgtaa tttaaccgcc aagggcactt tagctcgagt tattcagcat gagattgacc
     3301 atttaaatgg cattttattt attgattatt taaagtaatg cctatgacta ttatgccgcc
     3361 aaagaagatt agtttagtat tagctggttc gccgcagtta gctgtgcctg cttttgaggc
     3421 tttgtggcga gatgggcgct ataatattgt gggagtgctg acccaaccgg ataaaccagc
     3481 aggccgggga caaaaaatta ctccaccacc ggttaaagta tgggctcaaa gccataattt
     3541 gccagtttgg cagccggaat atttaccatc atggttgaca atagctcaag aaattaagcc
     3601 ggagatgatt gtcgttatgg cttatggcca atttatcccc tcttctttac ttaatcttcc
     3661 gtggttaggt tggattaatg tacatggctc actactgcct aaatatcggg gggctggtgt
     3721 attaacagcc ccaatactga acggagatag agaaactggt atttccctta ttcgattaac
     3781 taagaaatta gatgctggtc ctattattgc tagacgagtt attcccctgt cccctattga
     3841 aactagtggc agtttagcag ctaaaatagc taatgaggca gcgttaatgc tacctgatgt
     3901 tttatatcaa tatgctcaag gtttattagt gcctaaagag caagatgaaa gtcaggctac
     3961 ttacgtagga ttaattaaac cccaagatgc cagaattgat tggcgtaagt cggcagtcga
     4021 aattgagaga tttattaggg cgatgcagcc taagccagga gcttggacta tgtgggatgg
     4081 tcaacggtta aagattatag ctagtgaggt agctgattca gccggtttta ggccaggaca
     4141 agtggaacac atcagcaatc atttagtagt tggcactggt caaggcaatt taattgtaga
     4201 taaattgcaa ttagccggtc ggtcagtact aagcggagaa gaatttttga ggggttatcc
     4261 taatgtagtt agcgccgtgt ggtcttaata ggccttcctc tggacaggtg aagtagtttt
     4321 tgttataagt aatttattgc caccatcgtc tagtggttag gacacatggt tctcagccat
     4381 gtaactgggg ttcaactccc catggtggta ctttct
//
LOCUS       DAOOIS010000027         4324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_15892_length_4324_cov_44.594753, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000027 DAOOIS010000000
VERSION     DAOOIS010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15892_length_4324_cov_44.594753"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2960
                     /locus_tag="PK960_02580"
     CDS             <1..2960
                     /locus_tag="PK960_02580"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPD08080.1"
                     /translation="AVTALLRAFGYGTDEEIKKLFVGVDIADDVSYIEATMAKDLAKN
                     EAEGLKEVYKRIRPGDLATADNARQLIHSMFFRFDRYDFGRVGRYKLNQRFGFDIPNN
                     KETRILRPEDLVAIIKEIIRLNITQDPEDDIDHLGNRRVRAIGELLQNKTRVGLARME
                     RIVRDRMSILDLGEITPNKLINARPVISVVREFFMSSQLSQFMDQTNPLAELEHKRRL
                     SALGPGGLARERAGFEVRDVHSTHYSRICPIATPEGPNIGLVGHLASYARINDFGFIE
                     APYCRVLHDVQNTIDNTLGQTLRQDITWTDPHTKKRLILAKAGQVIDKALAQKLEDKL
                     GTLVTIPIVPVVTDEIMYLDASTEEKFTTTAFTIPVDKNGHFMVERAEGRRYGQPAIV
                     DVNEIDFIGVAANQIVSIGTSLIPFLEHNDGIRALMGTNMQRQAVPLVLAEAPIVGTG
                     IEARAAKDSGHAIVAPEDGIITYADSLTIELTGKSGQIHLFKLNKYLRSNASTCINQK
                     LIVEPGQKVKKGELLTDASAIDHGELALGNNVLVAFMTWEGFNYEDAVIISERLVRDD
                     TYSSIHIENYTIDVRDTKLGPEVITNDIPNISEEKLKDLDERGIVRIGAEVSSGDILV
                     GKITPKGETELSAEEKLIRAIFGEKAKDVRDSSLYLEHGEHGKVIDIKVFSRDQGDKL
                     ATGVIESVQVTVANLRKIKAGDKLAGRHGNKGVISRIVPMEDMPYLADGTPIDIILSP
                     LGVVSRMNLGQLLETHLGMAAHALGYKVATPALNGISEATIKAELKKANLPEDGKVQL
                     YDGKTGQPFDHTTTVGYKYMLKLNHMVEDKIHQRSIGPYSLITQQPLGGKAQFGGQRF
                     GEMEVWALEGYGAAHTLQEILTIKSDDVVGRSKAYESIIKGEPIKKLNVPESFNVLVR
                     ELKGLALDVQLLDEAGNIVELVNPMDDFNNRYDQNNNLEANAQVNEVIKEEIERHNDK
                     LEAEEGGEGLEII"
     gene            2986..>4324
                     /locus_tag="PK960_02585"
     CDS             2986..>4324
                     /locus_tag="PK960_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPD08081.1"
                     /translation="MSTPIKSNDFKAIKLKLASPEEILSWSHGEVTRPETINYRTQKP
                     EKDGLFCERIFGPSKDWECYCGKYKKIRYKGIICDKCGVEVTRSSVRRERMGHITLET
                     PVCHIWFLRGLSSKIGLALNLSAQAIEKVVYFADFIITNVNQELLAATTEQIQREYKS
                     KKKLFENDFIKGLAKLDKEKKAAQAAGQSSLEIQQLVDQTMEELTNEREAKMAELEEV
                     FAQTQRDLKELRVMNILSENDYQNLSLKYGHIFTAGIGAEAIRELLANIDLDKTIQSL
                     EAELKGAIDSKKEKIIKRLRFFKNLANNNLRPEWMILTVIPVIPPDLRPMVALDGGRF
                     ATSDLNDLYRRIINRNNRLKQLMELKAPEVICRNEKRMLQEAVDALIDNSIRRGKTVT
                     ASTGQKRSLKSLADSLRGKQGRFRQNLLGKRTDYSGRSVITVNPNLRLWQCGLP"
BASE COUNT         1311 a          697 c         1027 g         1289 t
ORIGIN      
        1 tagcggtgac agctctgctg agagcttttg gttatggcac tgacgaagaa attaagaaat
       61 tatttgtcgg ggtagatatt gctgatgatg ttagttatat tgaggccacc atggctaagg
      121 atttagcaaa aaacgaagct gaaggattga aggaagtgta taagcggatt cgtccaggtg
      181 acttagccac ggccgataat gctagacaac taattcattc tatgtttttc cggtttgacc
      241 gctatgattt tgggcgagtc ggccgctaca agcttaatca acggtttggt tttgatatcc
      301 ccaataataa agaaacacgc attttgcgac ctgaagattt agtagctatt attaaagaaa
      361 ttattcgctt aaatattaca caggatccag aagatgatat tgatcattta ggcaatcgtc
      421 gggtgagagc cattggtgaa ttactgcaaa ataaaactag ggtaggcctt gctagaatgg
      481 agcggattgt tcgtgaccgg atgagcattc tagatttagg agaaattact cctaataagt
      541 tgattaacgc tcggccggtt attagtgtgg tgcgggagtt ttttatgtca tcccaactat
      601 ctcagtttat ggaccagact aatcctctgg cggaactaga gcataaacgt cgtctttcgg
      661 ctttagggcc aggaggctta gcgcgggaac gggccgggtt tgaagtgcgc gatgttcata
      721 gcactcatta tagtcgcatt tgtcctatcg ctaccccaga aggaccaaat attggtttgg
      781 tgggtcactt agccagttat gcccgaatta atgattttgg ctttattgaa gcaccatatt
      841 gtcgggttct tcatgatgtt caaaatacta tagacaatac tttgggccag actttacgtc
      901 aagacattac ttggactgat cctcacacca agaaaaggtt aattttagct aaagccggtc
      961 aagtaataga taaagctcta gcgcaaaaat tagaagataa attaggtact ttagtaacta
     1021 tccctattgt gccagtagtc actgacgaaa tcatgtattt agatgcctcg actgaagaaa
     1081 aatttactac gaccgccttt actataccag tagataagaa tggtcatttt atggttgagc
     1141 gagcggaggg gcgacgttat ggccagccag ccatagttga tgttaatgaa attgatttta
     1201 tcggggtagc agccaaccaa attgtctcta ttggcacgtc actcattccc tttttagagc
     1261 ataatgatgg tatccgtgct cttatgggta ctaatatgca acgtcaggcg gtaccgttag
     1321 tgctggccga ggcgccgatt gttggtactg gcattgaggc tcgggccgct aaagattcag
     1381 gccatgctat tgtggcccca gaagatggga ttattactta tgctgattca ttaaccattg
     1441 agttaactgg taaaagtggc cagattcacc tttttaagct taataaatat ttgcggtcta
     1501 atgcctctac ttgtattaac caaaaattaa ttgttgagcc gggtcagaaa gttaaaaagg
     1561 gtgaactttt gactgatgct tcggccattg atcatggcga gttagcctta ggcaataatg
     1621 tgctggtagc ttttatgact tgggagggtt ttaattatga agatgcggtt attatttcgg
     1681 aacgtctagt aagagatgat acctatagtt ccattcatat tgaaaattac accattgatg
     1741 tgcgtgatac taagttgggt ccggaagtta ttactaatga tattcccaat attagcgaag
     1801 aaaaactaaa ggatttagat gaacgaggta ttgtccggat tggagctgaa gtatcaagcg
     1861 gtgatatttt agtggggaaa attactccga agggcgagac tgaacttagt gctgaggaaa
     1921 aattgattcg ggctattttt ggagagaaag ctaaagatgt tcgtgactct tctttatatt
     1981 tagagcacgg agaacatggt aaagttattg atattaaagt gttttcccga gaccaaggag
     2041 ataaattagc aaccggtgtg attgaatcag tacaagtaac ggtagctaat ttacgtaaga
     2101 ttaaggcggg ggataagtta gcaggccgtc atggcaataa gggggttatt tcgcgcattg
     2161 tgcccatgga agatatgccc tatttggctg atggcacacc tattgatatt attctctctc
     2221 ctttgggcgt agtttcaaga atgaatttag ggcaattatt agaaacccat ttgggcatgg
     2281 cagctcatgc tttgggctat aaagtagcta ccccggctct taatggaatt agtgaggcaa
     2341 ccattaaagc tgaacttaaa aaagctaatt taccggaaga tggtaaagtt cagctctatg
     2401 atggtaaaac tggtcaaccc tttgatcata cgaccactgt gggttataaa tacatgctaa
     2461 aacttaacca tatggttgag gataaaatcc atcaacgttc cattgggcct tattcgttaa
     2521 ttactcaaca gcctttaggg ggtaaagcgc aatttggtgg tcaaagattt ggggagatgg
     2581 aggtttgggc tctggaaggt tatggagcgg ctcatacttt gcaggaaatt ttaaccatta
     2641 aatcagatga tgtagttgga cgcagtaaag cctatgaatc tattattaag ggtgagccaa
     2701 ttaaaaaatt aaatgtgcca gagtcattta atgttttggt gcgtgaatta aaaggtttag
     2761 ccttagatgt tcaattatta gatgaagcgg ggaatatagt ggaactagtt aacccgatgg
     2821 atgattttaa taatcgttat gaccagaata ataatttaga agccaatgct caggttaatg
     2881 aagttattaa agaggaaata gaacggcata atgataaatt agaggcagaa gaaggaggcg
     2941 agggtctaga gattatttaa ttttgttttt tataattttt attatatgtc tactcccatt
     3001 aaaagtaatg attttaaggc gattaaatta aaattagcct caccggagga aattcttagc
     3061 tggtcgcatg gtgaagtaac tcggcctgaa actattaatt accggacgca gaagcctgaa
     3121 aaggatggtc ttttttgtga aagaattttt ggaccatcca aggattggga atgctactgc
     3181 ggaaaatata aaaagattcg ctataaggga attatttgtg ataaatgcgg cgtggaagtt
     3241 acgcggagca gtgtgcggcg cgaacggatg ggccatatta ctctagaaac tccagtatgt
     3301 catatttggt ttttgcgggg tttgtcttct aaaattggtc tagctttaaa tttatccgcc
     3361 caagcaattg agaaggtggt atattttgct gattttatta ttactaatgt gaatcaagaa
     3421 ttattagcag ctactactga acaaattcag cgggaatata aaagtaagaa aaaattgttt
     3481 gaaaatgatt ttattaaggg tttagctaaa ttagataagg aaaagaaggc agcacaagca
     3541 gcggggcaaa gctctctaga aattcagcaa ttggttgatc aaactatgga agaattaacc
     3601 aatgagcggg aagctaagat ggctgaactg gaggaagttt ttgcgcaaac tcagagagat
     3661 ttaaaggaat taagggtgat gaatatcctt tccgaaaatg attaccagaa tttaagttta
     3721 aagtatggtc atatctttac tgctggcatt ggggctgaag ctattcggga gcttttagct
     3781 aatattgatt tagataaaac cattcaaagt ttggaggctg agcttaaggg ggcgattgat
     3841 tctaagaagg agaaaattat taaacgttta cggttcttca aaaatttagc caataataat
     3901 ttacggccag aatggatgat tttaacagtt attccggtta ttccgcctga cttacgtcca
     3961 atggtcgctt tagacggtgg tcggtttgcg acttctgacc taaatgattt atatcgtcgt
     4021 attattaacc gcaacaatcg gctaaaacag ctgatggaat taaaagcacc agaagttatt
     4081 tgccgtaacg agaagagaat gctccaagaa gctgtggatg ctcttattga taattctatt
     4141 cgccggggga aaactgttac cgcttctact ggtcaaaaga gaagcctaaa atcattagcc
     4201 gattccttac gaggtaaaca gggacgtttt cgtcaaaatt tattaggcaa acggacagat
     4261 tattcgggtc gtagtgttat tacggttaat cccaatttac ggttatggca gtgcggtctg
     4321 ccca
//
LOCUS       DAOOIS010000028         4172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_16631_length_4172_cov_68.887054, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000028 DAOOIS010000000
VERSION     DAOOIS010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16631_length_4172_cov_68.887054"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="PK960_02590"
     CDS             <1..1494
                     /locus_tag="PK960_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPD08082.1"
                     /translation="IFTMTEPMKVLLNEGAPSFKVMRQAIEDGMVTMLQDGILKMLDG
                     QTSLEEVLRVVGKTDYIDDLYDIVVSQTLGRGLTIKEADLQFGEAIAKDINQAGTLLA
                     NKPTSEMLTAIMAGAIASKAADVHIDPIEGGVKIRYRLDGILHDIITLGSEHYVPLIG
                     EIKNLIGLSLSVKQPTFDGRFSMILPQERMDCRVSLITGGYGETVVIRLLSSQSISLD
                     LSDLGIRDISLSLIDKSLHKTKGIIITTGPTGSGKTTTLYALLRKLNTPEVKIITIED
                     PIEYHLDGVMQTQIDSAGGYTFAAALRSLMRQNPNIIMVGEIRDPETAKTAIEASLTG
                     HLVLSTIHANSAAGAIARFAGLGVDRPMLASALECSIGQRLVRRLCPYCKKPITPSAD
                     IWEQAEPAWEFLRKRPDLKLPDKPQFYGPVGCPQCRGIGYNGRLGIYEVIIITPEMQK
                     LIQQPLTTEAELEELAIKQGSLLMTHDGLIKAANGETSLEEIWRVAG"
     gene            1496..1993
                     /locus_tag="PK960_02595"
     CDS             1496..1993
                     /locus_tag="PK960_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HPD08083.1"
                     /translation="MTILVFSDLHDNLPNLETCFNWANNNQVDAAIFLGDLTNADTLL
                     ELARLWPKEFYFIQGNCDTYLTSDIPSYPQWHNVGRYGQIIVDKRHIGLCHEPRYWEE
                     VLREGSVEVLFYGHSHKPWEELDNNTWLVNPGTLGGVYYPASFALYDTVNNVWKLKTL
                     SQLKI"
     gene            1990..3336
                     /locus_tag="PK960_02600"
     CDS             1990..3336
                     /locus_tag="PK960_02600"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPD08084.1"
                     /translation="MSWLILAVGVLWLILSWSNLKLALSLLFGLLPVYLLRFKVGFVP
                     STVLEEFIVITTMVWLIKSWPRPWKNIIRKPKNWHPYPFTWEIIIILIIAWIAVAISG
                     YSLPALGIWKAYFIEPILVFVIALNVWQKPQDIVNALLFLAGSVTLIGLLAGYQYFTG
                     QLIFNPEWASVTGRRATSFFGYPNAIGLFVAPVSLALLGCLIYLRKERSKLFTWSNIL
                     LAASIILSWLALYWAHSEGAIVGLIIAIIVMIFLSSRGGKVVSIILTALLVIVTLENP
                     GIYRKISLADESGQIRRAQWQETWEMLRDGRLFTGAGLANYQSSVKPYHETGIWLKPV
                     DKSEKPVWQPVEIYLYPHNIILNFWSELGLLGMIIFMWLWFKSLFINCRNFCGYHLKN
                     NLAWLNWGLFGAWLAAFIHGLVDVPYFKNDLAVLFWLGLALTGWLNWQLRGKLKSK"
     gene            3339..4070
                     /locus_tag="PK960_02605"
     CDS             3339..4070
                     /locus_tag="PK960_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPD08085.1"
                     /translation="MKQQLTKYLASHGPWSRRQAESLIRAGRVTVDDQPAHLGEIIIN
                     PHTIIKVDGHPIANQEPAPVYFMVNKPVGYTCTNKQLPHEKNIFELVNYPGRLFVAGR
                     LDKNSHGLVLLTNDGILAAKLTHPRFTHPKIYEVTVRPGITSANFIKLQTFLSQGHKL
                     YDGQSLARAKISRQLASDRLEIILEQGQKRQIRLLLKLAGYEVIDLKRTGLGPLKLGR
                     LPIGAVRSLNTAEIDRLKQLIQDKQ"
     gene            4086..4171
                     /locus_tag="PK960_02610"
     tRNA            4086..4171
                     /locus_tag="PK960_02610"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4120..4122,aa:Ser,seq:cga)
BASE COUNT         1123 a          734 c          944 g         1371 t
ORIGIN      
        1 atttttacta tgaccgagcc gatgaaggtt ttgcttaacg aaggagcgcc ttcgtttaag
       61 gtaatgcggc aagctattga agatgggatg gttactatgc tccaagacgg aattttaaaa
      121 atgcttgatg gccagacttc actggaagaa gttttgaggg tagtgggcaa gacggattat
      181 attgatgact tatatgatat tgttgtctcc cagaccctgg ggcgcgggtt aactattaaa
      241 gaggcggatt tacaatttgg tgaggccata gctaaagaca taaatcaggc aggtacatta
      301 ttagccaata aaccaaccag tgagatgctc accgccataa tggctggcgc tattgccagt
      361 aaagccgctg atgttcatat tgatcctatt gaaggtggtg tgaagattcg ctaccgtctt
      421 gacggtattt tgcacgatat tattaccctg ggttcagaac attatgttcc gttaattggg
      481 gagattaaaa atttaattgg tttgtcgctc agtgttaaac agccaacttt tgatggtcgt
      541 tttagtatga ttttaccgca agagcggatg gactgtcgtg tttctttgat cactggcggc
      601 tatggagaga cggtggttat ccgtttactg tccagtcaat ctattagcct agacttgtct
      661 gacctgggca ttcgcgatat ttctttgtcc cttattgata aatctcttca caagactaaa
      721 ggcattatta ttactactgg ccctactggt tcaggcaaga cgactactct ttatgcttta
      781 cttagaaaat taaatactcc agaagtaaaa attattacga ttgaagaccc tattgaatat
      841 catttggacg gagtgatgca gacccaaatt gattcagctg gtggctatac ttttgcggca
      901 gcattgcgct cgttaatgcg tcagaatcct aatatcatta tggtgggtga aattcgtgat
      961 ccagaaacgg ctaaaacagc cattgaagct agcttaactg gtcatctagt gctatctact
     1021 attcatgcta atagtgcggc tggcgcaatt gctcggtttg ccggattagg cgttgatcgg
     1081 ccgatgctgg ctagcgccct ggagtgttct attggccaac gattagttag acgtttgtgt
     1141 ccttactgta aaaaacccat tactccatca gcagatattt gggaacaagc tgaaccagct
     1201 tgggagtttt tacgtaagcg gccagactta aagttgccgg ataagcctca gttttatgga
     1261 ccagttggtt gtcctcagtg tcgtggtatt ggctataatg gccgtctggg tatttatgaa
     1321 gttattatta ttactccaga aatgcaaaaa ttaattcagc aacccttgac tactgaagct
     1381 gaacttgagg aattggctat taaacaaggc tcactgctaa tgacccacga tggtttaatt
     1441 aaagcagcta atggcgagac aagtttagaa gaaatttggc gagtcgctgg ttaatatgac
     1501 aatcttagta tttagtgatc ttcatgataa tttacccaat ttagagactt gttttaattg
     1561 ggcgaataat aatcaagtcg atgcggctat ttttttggga gatttaacta atgccgatac
     1621 gcttttagag ctagctagac tttggcctaa ggagttttat tttattcagg gcaattgtga
     1681 tacttattta accagtgata ttcccagtta tcctcagtgg cataatgttg gtcggtatgg
     1741 ccaaattatt gtagataaac ggcacattgg tctttgtcac gaaccaagat attgggaaga
     1801 agtattaaga gaaggatcag tagaagtatt gttttacgga catagtcata aaccatggga
     1861 ggaattagat aataatactt ggctcgtgaa tcctggcaca ctaggaggag tttattatcc
     1921 ggcctctttt gccctttatg atacggttaa taatgtttgg aagcttaaga cattaagtca
     1981 gttaaaaata tgagctggtt aattttagcc gtcggtgttt tgtggcttat tttgtcatgg
     2041 agcaacttaa aattagctct atctttgcta tttggtttat tacctgttta tttattgcgt
     2101 tttaaggtgg gttttgtgcc ttctactgtc ttagaagaat tcattgtaat taccaccatg
     2161 gtttggctca ttaagtcttg gcctcggccc tggaagaata ttatccgtaa acccaagaat
     2221 tggcatcctt atccttttac ttgggagata attatcatat taattattgc ctggatagcc
     2281 gttgctatta gtggctatag tcttccagcc ctgggaattt ggaaggctta tttcattgag
     2341 ccaattttag tttttgttat tgcccttaat gtttggcaaa aacctcaaga cattgttaat
     2401 gccctattgt ttctagcggg tagtgtaacc ttaattggtt tattagccgg ctaccagtat
     2461 tttaccggcc agctcatttt taatcctgag tgggcgtcag ttactggtcg gcgggctact
     2521 tctttttttg gctaccccaa tgctattggt ctctttgtgg ccccagttag cttagcactt
     2581 ttgggttgcc ttatttattt aagaaaagaa cggtctaaac tatttacttg gtctaatatt
     2641 ttactagccg ctagtattat tttgagttgg ctggctcttt attgggccca ttccgaaggc
     2701 gctatagttg gattaattat agctattata gttatgattt ttctttctag ccgtggtggt
     2761 aaggtggtat ctattatttt gactgctctt ttggttattg ttactttaga gaaccctgga
     2821 atttatagga aaatttcttt agccgatgaa tcaggccaaa tccgtcgagc tcaatggcaa
     2881 gaaacttggg agatgttacg agacggccga ttatttactg gggctggtct tgctaattat
     2941 caatctagtg tgaagccata tcatgaaaca ggtatttggc ttaagccagt agataagtca
     3001 gaaaaaccag tctggcagcc ggtagagata tacctctatc ctcacaatat tatcttaaat
     3061 ttttggagtg aattggggct actggggatg ataattttta tgtggctatg gtttaagtct
     3121 ctatttatta attgtcgcaa tttttgtggt tatcatctta aaaataattt agcttggctt
     3181 aattggggat tatttggggc ttggctggct gcttttatcc atggtctcgt agatgtgcct
     3241 tattttaaaa atgatttagc agtattattt tggctaggcc tagcgcttac tggttggctt
     3301 aactggcaat taagggggaa gttaaaaagc aaatagccat gaaacagcaa ttaactaaat
     3361 atttagccag tcatggccct tggtccagac gacaagccga atctttaatt agagccggac
     3421 gagttactgt ggatgaccag ccagctcatt taggggagat cattattaac cctcacacaa
     3481 ttattaaggt tgatggtcac ccaatagcta atcaggaacc ggctccggtt tatttcatgg
     3541 ttaataagcc ggtaggctat acttgtacta ataagcaatt gcctcatgaa aaaaatattt
     3601 ttgagttagt gaattatccg gggcggcttt ttgtggctgg ccgtttagat aaaaatagcc
     3661 atggcttagt tcttttaacc aatgatggaa ttttagcagc taaattgacc caccctcgtt
     3721 ttactcaccc taagatttat gaggtcactg ttagaccggg gattacttct gctaatttta
     3781 taaaactaca gacatttttg tcccagggtc ataagttata tgacggccaa agtttggcta
     3841 gagctaaaat aagccgtcaa ttagctagtg atcggttaga aattatttta gagcaaggtc
     3901 aaaagagaca gattcgttta ttgctaaaat tagcaggcta tgaagtgatt gacttaaaaa
     3961 gaactggact tggtccttta aagttgggtc gtctgcccat tggggcggtt cggtcgctta
     4021 atacggcaga gattgacaga ttaaaacaac taattcaaga taaacaatga attagttgac
     4081 ttaagggagg agtgcgagag tggttgaatc gggcgctctc gaaaagcgct atgcctatat
     4141 ggcatcaagg gttcgaatcc cttctcctcc gc
//
LOCUS       DAOOIS010000029         3860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_18271_length_3860_cov_56.756899, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000029 DAOOIS010000000
VERSION     DAOOIS010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18271_length_3860_cov_56.756899"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PK960_02615"
     CDS             <1..489
                     /locus_tag="PK960_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPD08086.1"
                     /translation="IIDEGQPFSVIVDYAFEPVALSKIYETLELVPHKKIIHVLGSAG
                     GGRDVARRPKLGQIAGRLADIVIVTNEDPYDDDPRVIIEQVAVGAEYAGKERNKNLFT
                     ILDRREAIAKALSLAKKGDIVLITGKGSEQAICLAAGRKMPWDDRQVVREELAKLQFS
                     ES"
     gene            670..2835
                     /locus_tag="PK960_02620"
     CDS             670..2835
                     /locus_tag="PK960_02620"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPD08087.1"
                     /translation="MVLAIFWFIPAAGLLALLTAFILFVQMKRKEPGNEKMQKIAGLV
                     RQGAMAYLRQQYKVVAIVFIVASIFFASAAYGFNLMSRYTPFAFLTGGIFSLLCGFIG
                     MVAATHAASRVTKAVQDSLNNGLQLALRSGAILSFSVVGFALVDVSVWFWVLNYFFDG
                     APEAKMLTITTIMLTFGMGASLQALFARVGGGIFTKAADMGADLVGKNEFGLEEDDPR
                     NPAVIADNVGDNVGDVAGMGADLYESYAGAILASAALMASASHGLSEATQMKYVLLPM
                     VIGALGVIFSVITLLTVKAKNDFGMKQLVGSINKGIWLSSILIAVATFIVTAWLGLQN
                     WLGLSLAVSGGLVVGVVIGFWVNYQTSQGFRPTQRVAESAQTSPATVITSGLELGMKS
                     TWVPVVAVCLGMAGAFYCASGFNFSNVSLGLAGISLAAVGMLANLGFVLATDAFGAIA
                     DNAGGNAEMAKLPDGVRKRTDMLDAVGNTTAAIGKGFAIGSAALTAMALLASYIEEVK
                     LALIRAGNTSLQNIPITKINIIDLVNYYQINIMNPKFIIGAFIGAMIVLVFIGLTTGA
                     VSKVARTMVAEVRRQFSNKKILNGLEEPDYQRCIRISTKGAQTAMLTPALLAVVAPII
                     IGVLLGVAGVMGLLLSAMTTGFGMAVFLANSGGAWDNAKKYVEEGNFGGKGSQAHKAA
                     VIGDMVGDPCKDTSGPALNILIKLMSMVAIVTAGITIIF"
     gene            3238..>3860
                     /locus_tag="PK960_02625"
     CDS             3238..>3860
                     /locus_tag="PK960_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPD08088.1"
                     /translation="MSHPSQSVHTIGQRRYFIDRSALMSLPDLTEVQKKSYSWFLKEG
                     LADLFDEISPISDFMGRDLELYLTDYYFDEPKFNETESKAKNITYEASLRVRARLVNK
                     KTGQSMTQEVFLGDFPLMTENGTFIINGIERVVVSQLIRSAGVIFNAEDIRGRKYYGA
                     KIIPNRGAWIEIETDVNNVIWARIDRKRKVAVTALLRAFGYGTDEEI"
BASE COUNT         1033 a          653 c          942 g         1232 t
ORIGIN      
        1 attattgatg aaggccagcc atttagcgtg attgttgatt atgcttttga gccggtggct
       61 ttgtctaaga tatatgagac tttggagtta gtgcctcata aaaaaattat ccatgtttta
      121 ggttcagccg gcggggggcg tgatgttgcc aggcgaccta aattaggcca gattgctggc
      181 cggttagctg atatagtaat tgtgaccaac gaagaccctt atgatgatga cccgcgggtt
      241 attattgaac aggtggcagt cggagctgaa tatgctggca aagaaagaaa taaaaattta
      301 tttactatcc tggatcgtcg cgaggctatt gctaaggcct tatctttagc taagaagggt
      361 gatattgttt taattaccgg caaggggagt gagcaggcta tttgcctggc tgcaggccgt
      421 aagatgccct gggacgatcg gcaggtggtg cgagaagaat tggctaagct gcaattttca
      481 gaaagttagg tttttatctt tacttgagga ataaataata accttgccat ctttttttga
      541 taaattatta ttaatagctg gcttttgttc aatgtggact agggcgctat atgggtcttg
      601 ctattttctg tgttatatga taagaagcct tataattgtt cattaaaaat aaaatacagg
      661 aggaacaaaa tggttttagc aatattctgg tttatcccgg ctgctggtct tttagctctt
      721 ctaactgctt ttattctttt cgtccagatg aaaagaaaag aaccgggcaa tgaaaagatg
      781 caaaagatcg ctgggctagt aagacagggg gctatggcct atttgcggca gcaatataag
      841 gtcgttgcta ttgtgtttat tgtggctagt atattttttg ctagcgctgc ttatggtttt
      901 aacctaatga gtcgatatac cccatttgct tttcttacgg gaggcatatt ctccctgctt
      961 tgtgggttta ttggcatggt agccgctact catgcggctt ctagagtaac taaggcggta
     1021 caagattcac taaataacgg cttacaactt gccctaagaa gtggggcaat tttaagtttt
     1081 agtgtggttg ggtttgcctt agtggatgtg tcggtctggt tctgggtttt aaattatttt
     1141 tttgatggtg cacccgaggc caagatgctt accatcacta ccattatgtt gacttttggc
     1201 atgggggctt ctttacaggc cttatttgct agggttggcg gtggtatttt tacaaaagcc
     1261 gcggacatgg gagctgatct ggttggtaaa aatgaatttg gactagagga agatgatcca
     1321 cgcaatccag cagttattgc cgataatgtc ggtgataatg tgggtgatgt ggctggcatg
     1381 ggcgcagatc tatatgagtc ctatgctgga gctattttag cttcagctgc cttaatggct
     1441 agcgcgagtc atggtttatc tgaggccact cagatgaaat acgtgctatt gccaatggtg
     1501 attggtgctc taggagtgat attttctgtc attacactcc ttaccgtgaa ggctaaaaac
     1561 gactttggaa tgaaacagtt agttgggtca attaataaag ggatctggct aagttctatt
     1621 ctgatagccg ttgccacgtt tattgtaact gcctggctag gcctacaaaa ttggttaggt
     1681 ttatctctgg cagtaagtgg cggcctagtg gttggggtgg taataggatt ttgggttaac
     1741 taccaaacct cgcaaggttt tagaccaacc cagcgagttg ccgaaagcgc tcaaacatcg
     1801 ccagccaccg ttattactag tggattagag ttgggtatga aatctacttg ggtgcctgtg
     1861 gtggcggtat gtttgggtat ggccggggcc ttttattgtg cctctggatt taattttagt
     1921 aatgtcagct taggactggc cgggatttcc ctggcagccg ttggtatgtt agctaacctt
     1981 ggtttcgtgt tagcgacgga tgcctttgga gctattgctg acaatgctgg cggtaatgcc
     2041 gaaatggcaa aattaccaga tggtgtgcgg aagagaaccg acatgctaga tgcagttggt
     2101 aatactaccg cggccattgg taagggattt gccattggtt cggccgctct aacggccatg
     2161 gctttattag cttcatatat tgaagaagta aagctggcct tgattagagc gggaaatact
     2221 agcctgcaaa atattcctat aacaaaaatc aacattattg atctagtaaa ttattaccag
     2281 atcaacataa tgaatcctaa atttattatt ggtgccttta ttggtgccat gatagtttta
     2341 gtgtttattg ggttgacaac tggggccgtc agcaaagttg ccagaacaat ggtggctgag
     2401 gttcgccgtc aattcagtaa caaaaaaatt ctaaacggat tagaggaacc cgattaccaa
     2461 agatgtattc ggatttctac caagggagca cagacagcaa tgcttactcc ggccttgttg
     2521 gctgttgtag ctcctataat aataggagta ttattgggtg ttgccggtgt tatgggcttg
     2581 cttttaagcg ccatgaccac cggttttgga atggcggttt ttttggctaa ttccggaggg
     2641 gcttgggata atgctaagaa atatgttgaa gagggtaatt tcggcggcaa gggttcccag
     2701 gcccacaaag cagcagttat cggcgacatg gtcggagatc cctgcaagga tacatccggc
     2761 ccggccttaa atattttgat caaattaatg tcgatggtgg ccattgttac cgccggcatt
     2821 actattattt tttaacaact atataactat ataacccgac gtaagtcggg ttttttaatt
     2881 aataggctaa cctttatgaa taatttatat aaaatatata aatataataa caaaaattga
     2941 tgacgctatt ttgttgtggt atgaaatcct tgacagcctg ttagcatttt gctaatctgg
     3001 aaacattaga ttggctagac atcacaagat tgattgtcaa tctcaattct aagcttagat
     3061 cagagaaaaa catttaatga ccattgacta ttgttgggca agttcaaggt cattttattg
     3121 ttagtctttg gttggagcca taagataata cccctatcca tggcttaaat taaacaattg
     3181 gcaattttta ttaactcatc agccgtttgg ccttgagaat tttttgttat aaaaaatatg
     3241 tcgcatccct cccaaagtgt tcacactatt ggtcaacgtc gctattttat tgaccgtagt
     3301 gctttaatgt ccctgcctga tttaactgaa gtgcaaaaaa aatcatactc ctggttttta
     3361 aaagaaggtt tagctgattt atttgatgaa atttcaccga tcagcgattt tatggggcgt
     3421 gatttggaac tttatttaac agattattat tttgatgaac ctaagtttaa tgagactgag
     3481 agcaaagcta aaaatattac ttatgaagcc tcgttaagag tcagggcgcg gttagttaat
     3541 aaaaaaactg gtcaatctat gactcaggaa gtatttttgg gtgattttcc attgatgacc
     3601 gaaaatggca cttttattat taatggaatt gagcgggtag tggtttctca attgattcgt
     3661 agcgctgggg ttatttttaa tgctgaagat attcgaggcc gtaaatatta tggcgctaag
     3721 attattccta atcgtggagc ttggattgaa attgaaactg atgttaataa tgtaatttgg
     3781 gcgcgcattg atcgtaagcg caaagtagcg gtgacagctc tgctgagagc ttttggttat
     3841 ggcactgacg aagaaattaa
//
LOCUS       DAOOIS010000030         3840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_18394_length_3840_cov_47.557992, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000030 DAOOIS010000000
VERSION     DAOOIS010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18394_length_3840_cov_47.557992"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..433)
                     /locus_tag="PK960_02630"
     CDS             complement(35..433)
                     /locus_tag="PK960_02630"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08089.1"
                     /translation="MAKKLVPIKKNIVWLIGIPILSMTLIHSNFFLLSARAEEDKSFG
                     LDAAATKAGLKTGVTLQSRAGQIVGSVLALVGILFFVLIIYGGILWMTAGGNDTQLKN
                     AQKTITTAVIGLIIVLSAYAITMFIGQALI"
     gene            complement(536..937)
                     /locus_tag="PK960_02635"
     CDS             complement(536..937)
                     /locus_tag="PK960_02635"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08090.1"
                     /translation="MKKWLIGIMSLVWATSLISLSPVTVNAQNLDVWGNTQNSYANIG
                     LSDTDPRDVVANVIKVVLGFLGTIAVVLIIVAGFQWMTAAGSEDKIAKAKKIMTAAVI
                     GLVIVLMAYALSTFVINAIISATNPSTAVPD"
     gene            complement(1008..1385)
                     /locus_tag="PK960_02640"
     CDS             complement(1008..1385)
                     /locus_tag="PK960_02640"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08091.1"
                     /translation="MKKWLIGIMSLVWATSLISLSPVTVNAQNLDVWGNTQNSYANIG
                     LSDTDPRDVVANVIKVVLGFLGTIAVVLIIVAGFQWMTAAGSEDKIAKAKKIMTAAVI
                     GLVIVLMAYALSTFVINAIISAV"
     gene            complement(1418..1807)
                     /locus_tag="PK960_02645"
     CDS             complement(1418..1807)
                     /locus_tag="PK960_02645"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08092.1"
                     /translation="MNLIKKFIPTLLLAQCLIINLLTIQPVKAQVNLWGEGNIATCAF
                     NNIGFRERDPRQIVVDVIKIILGFLGTIAVVLILYAGFIWMTAGGKPDNVAKAQKIIS
                     AAVIGLIIILASYGLTMFITKALSNAI"
     gene            1955..>3840
                     /locus_tag="PK960_02650"
     CDS             1955..>3840
                     /locus_tag="PK960_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HPD08093.1"
                     /translation="MPIPKLVVRKPSHTYTAYVNRTGHRSSSIVKSRQPNTRRPTRRH
                     LNWPKIIKFFGALLILFLMISVFAFWWLSRDLPSPNKLLEREIAQSTKIYDRKGETIL
                     YEIHGDQKRTLVNLDQIPDYVKEATIAIEDKDFYNHGAFSLWAMFRTAVTNVLFHRRA
                     GGSTLTQQFVKNALLTSEKTYTRKIKELILAYRIEKKFSKDEILQMYLNEIPYGSNAY
                     GVEAASQRYFGKSISQVNLAEAAILAALPQAPTRYSPYGPNKETLIARQRYILDLMAE
                     QGYISKEEAAEAKDFPLQFKPLQENGNIIAPHFVMEIKGQLAEKYGEKILEQGGLKIY
                     STIDIEKQKIAEEAVTTQAEKNAKNFGASNAALFSMDAKSGDILAMVGSRDYFNDEID
                     GQVNLTLRPRQPGSSFKPIVYATAFMKGYTPRTVLFDVITNFSLDPDNPYEPHNYNGQ
                     EYGPVIMKKALAGSLNIPAVKTLYLAGLDDVLAVAKNLGYTTLNDKSRFGLSLVLGGG
                     EVKLAEHVAAYSAFARDGEMVKPRYILKVEDKDGKILEEIESEHVKVLEPQIAREIND
                     ILSDNDERMYIFGAKNHLTLNDRPAAVKTGTTNDYKDAWTIGYTPSIITGVWVGNNDN
                     KSMS"
BASE COUNT         1217 a          711 c          711 g         1201 t
ORIGIN      
        1 taaaattttc ttacgtttca tagtgacgca taaatcatat caaggcttgc cctataaaca
       61 ttgttatggc ataagctgaa agtactataa taaggccaat aactgccgtt gtaatggttt
      121 tttgggcgtt ttttagctga gtatcattac cgccagcagt catccacaga atgccgccat
      181 agattattaa aacaaaaaat aatatcccta ctaaggctaa aacagagcca actatttgtc
      241 cggctctgga ctgaagcgta accccagttt ttaaaccagc cttcgtggct gcggcatcaa
      301 gaccaaaaga tttatcttct tctgccctgg cactaagtag aaagaaatta ctgtggatta
      361 atgtcattga caggataggt attcctatta accacactat attcttttta atgggaacta
      421 attttttagc catgtaagac tatttaaaaa ttttgtctca gcataattac tatattatac
      481 tgaggcaaaa tttgccaaaa cattttggca aattttattt aataacagct aacttttagt
      541 cgggtaccgc cgtagacgga ttagtcgcgc taattatagc attaataacg aaggtgctta
      601 aagcgtaagc cattaaaaca attactaggc caataactgc cgcggtcatt atttttttag
      661 ccttagcgat tttgtcttca ctgccagctg ccgtcatcca ctgaaagcca gctacaataa
      721 ttaggacgac agcaatagtt cctaaaaagc caagcactac tttgattaca ttagccacaa
      781 catcgcgagg atcagtgtcg gatagtccta tatttgcata ggagttttga gtattacccc
      841 aaacgtctaa gttttgcgca ttgactgtga ctggggataa agagattaaa cttgttgccc
      901 agactagcga cataatacca attaaccatt ttttcataat tttttgttaa ttattaatta
      961 taaaaatttt taaattgcat tgcttaatgc tttagttata aacattgtta gaccgcgcta
     1021 attatagcat taataacgaa ggtgcttaaa gcgtaagcca ttaaaacaat tactaggcca
     1081 ataactgccg cggtcattat ttttttagcc ttagcgattt tgtcttcact gccagctgcc
     1141 gtcatccact gaaagccagc tacaataatt aggacgacag caatagttcc taaaaagcca
     1201 agcactactt tgattacatt agccacaaca tcgcgaggat cagtgtcgga tagtcctata
     1261 tttgcatagg agttttgagt attaccccaa acgtctaagt tttgcgcatt gactgtgact
     1321 ggggataaag agattaaact tgttgcccag actagcgaca taataccaat taaccatttt
     1381 ttcataattt tttgttaatt attaattata aaaattttta aattgcattg cttaatgctt
     1441 tagttataaa cattgttaga ccataagaag ccaaaataat tattagacca ataactgccg
     1501 cggagataat tttttgagcc ttagccacat tatctggttt accgccggca gtcatccata
     1561 tgaagccagc atagagtatc aatactacgg cgatagttcc taaaaatcct aatataattt
     1621 taataacatc aactactatt tgccgtggat ccctttctct aaaaccaata ttattaaaag
     1681 cgcaagtagc tatattacct tctccccata gattaacctg agccttaact ggctgaatag
     1741 ttaatagatt gattattaaa cattgagcta gtagaagggt gggtataaat ttttttatta
     1801 ggttcataaa ttaactttat tgaaatataa aaaatcaatt cgccattatt ataacatagc
     1861 cttatataag ctagcaaggg ctgtggcctt ctagctactt ttaattatat ataattattt
     1921 gctataattc aatagacaaa gattattttt tattatgccc atacctaaat tagttgtccg
     1981 caaaccctct catacctata cagcatatgt taatcggacc ggccatcgtt catcttcaat
     2041 tgttaaatca aggcaaccca atactcgacg gccgactcgc cgccacctta attggcccaa
     2101 gattatcaag ttttttgggg cgttgcttat tttattttta atgatcagtg ttttcgcctt
     2161 ctggtggttg tcacgtgatt tgccatcacc taataaattg ctagagagag agatagctca
     2221 aagtactaag atctatgacc gtaagggtga aactatcctt tatgagattc atggtgatca
     2281 aaaaagaaca ttggttaatc ttgaccaaat tcccgattat gtgaaggagg ccactattgc
     2341 cattgaagat aaagattttt ataaccatgg cgcttttagt ttatgggcca tgttccgcac
     2401 tgctgtcact aatgttttgt tccatcggcg ggctggtggt tctactttaa ctcagcagtt
     2461 tgtgaaaaat gccctattaa cttcagaaaa aacatatacc cgcaagatta aagagttaat
     2521 tttagcttac cggattgaaa agaaattttc taaagatgaa attttacaga tgtatttaaa
     2581 tgaaattcct tacggctcta atgcttatgg cgttgaagct gctagtcagc gttatttcgg
     2641 taagagcatt agccaggtta atttagccga ggcggcaatt ttagcggctc tacctcaagc
     2701 cccaacccgt tattcaccct atggccccaa taaagagact ctaatagctc ggcaacgtta
     2761 cattttagat ttaatggccg aacaaggtta tattagtaaa gaggaagcag ctgaagctaa
     2821 agattttccc ttacagttta agcccctaca agaaaacggc aacattattg ctcctcattt
     2881 tgtgatggaa atcaagggcc agctggctga gaaatatggc gaaaaaatac tagaacaagg
     2941 aggattaaag atttattcta ctattgatat agaaaagcaa aaaattgctg aagaggcagt
     3001 aactactcag gctgaaaaaa atgctaagaa ttttggcgct agtaatgccg ccctattttc
     3061 tatggatgct aaatcaggag atattctagc gatggtaggt tccagagact attttaatga
     3121 tgagattgat gggcaggtta atttaacctt aagaccccga cagccaggat catcttttaa
     3181 gcccatagtt tatgccactg cttttatgaa aggttatacg ccacggacgg ttttatttga
     3241 tgttataact aatttttctt tagacccaga taatccttat gagccacata actataatgg
     3301 tcaggaatat ggtccagtta tcatgaaaaa ggccttagct ggttcgctta atatcccagc
     3361 tgtcaaaact ctttatttgg cagggctaga tgatgtttta gctgtagcta aaaatttagg
     3421 ctatactact ctaaacgata aaagccgttt tggtttatct ttggttttgg gaggtggcga
     3481 agtaaaattg gcggaacacg tagctgctta tagtgctttt gcgcgcgacg gagagatggt
     3541 taagccacgc tatatattaa aagtggaaga taaagatggt aaaattctcg aagaaatcga
     3601 gtcggaacat gttaaagttt tagagccgca aattgccaga gagataaatg atattcttag
     3661 tgacaacgat gaacgtatgt atatttttgg ggctaaaaat catttaactc ttaatgatcg
     3721 gccggcagcc gttaaaactg gcactactaa tgattacaaa gatgcttgga ctattggtta
     3781 caccccgtca attattaccg gggtttgggt tggcaataat gacaataaat caatgagtaa
//
LOCUS       DAOOIS010000031         3584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_20165_length_3584_cov_63.038254, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000031 DAOOIS010000000
VERSION     DAOOIS010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20165_length_3584_cov_63.038254"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1741
                     /locus_tag="PK960_02655"
     CDS             2..1741
                     /locus_tag="PK960_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD08094.1"
                     /translation="MTKLLKYLTPYIWPLLLLALLIYCQASATLALPDYMARIINQGI
                     LGQNTGVIFHNGLIMILIAIGGGLATVGVGYLSSRIGTGFARDLRTMIFTKIEDFSLV
                     EFNKFSTASLITRSTNDVQQIQMVFIMICRIALLAPLMGILSTFKAYHLAPSMTWIMA
                     VAVVALIAIVIVLFQIVVPKFTKLQKLVDKLNLVTREILTGLRVIKAFGKEDYETQKF
                     DKVNQDLTRLHLLVNRLMVIIQPVMMLIMNFMMIAIVWLGAHHIGAGDLQVGSMLAFL
                     QYSMQAVSAFLMISIVFINLPRAAVSANRIAEVLETEIQIKDPTNPVRAHRRGGKVEF
                     KNVTFTYPGAAEPVLHNISFVALPGETTAFVGSTGSGKSTIINLIPRFYEVTEGQVLV
                     DDVDVRAMRQKDLRARIGYVSQKAMLFSGTIRDNITYGNPNATQEEILRAAATAQALE
                     FIKELEGKFDSSIAQAGTNFSGGQKQRLAIARALAKNPELYLFDDSFSALDFQTDKAL
                     RTALKEEAQGKTVLIVTQRIATIMNADKIVVIDKGEIVGQGTHETLLKTCKVYQEIAA
                     SQLAPEELARVLK"
     gene            1894..>3584
                     /locus_tag="PK960_02660"
     CDS             1894..>3584
                     /locus_tag="PK960_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD08095.1"
                     /translation="MRKLIKYLKPYYVSIMVVLILAMASTVFTIISPKILGDITNEIV
                     AGIKNITQGGIDFSAILHLITILVGLYLIGALCGYLQGWIMTGVAQKVTYNLRKSIAL
                     KINRLPLRYFDTQSHGDILSRVTNDVDTVSHSFNQSLTQVVTAVTTIVGILIMMIIIS
                     WQLTLVAVIVLPLGFLLARFIIKKSQKYFFNHQVSLGEINGHIEEMFSGHTIVKAFNG
                     EKKSISTFRKINNKLYNNAWKSQFFSGLLHPIMNFLDNAGFVAVSVLGGWFAIRGKMT
                     IGSIQAFIQYLNQFNRPIIQTASIANVFQSTAAAAERVFEFLEEEEELPDPAQPKELK
                     TVKGAVDFVNVVFGYTPEKIIINNFSATIKPGQKIAIVGPTGAGKTTIVNLLMRFYEV
                     DSGAIKIDGVDIKDLRRSDLRKLFGMVLQDTWLFSGTIEENIAYGKDNVSHEEVVKAA
                     QAAYADHFIHTLPGDYQMEISEEADNISQGEKQLLTIARAILANAPMLILDEATSSVD
                     TRTEALIQAAMDKMMEGRTSFIIAHRLSTIRNADLILVMRDGHIVEQGTHKQLLA"
BASE COUNT          998 a          620 c          795 g         1171 t
ORIGIN      
        1 catgacaaag ctcttaaaat atcttactcc atatatctgg cccctgcttt tgctagctct
       61 tttaatatat tgtcaggctt cggccacctt ggctttgccg gactatatgg ccagaatcat
      121 aaaccagggc atactcggtc aaaataccgg cgtaattttt cataacggct taattatgat
      181 tctcattgct attggcggtg gtttagccac ggtgggagtg ggttatttat cttcccgtat
      241 tggtacgggg tttgctcgag atttgcgcac catgattttt accaagatag aagatttttc
      301 tctagttgag tttaataaat tttccactgc ttcgcttatt actcgcagta ctaatgatgt
      361 tcagcagatc cagatggttt ttataatgat ttgccgtatt gctttactgg ctcctttaat
      421 ggggatactg tcgactttta aggcttatca tttagcgccg tcaatgacct ggattatggc
      481 ggtagcagta gtggctttaa ttgccatagt aatagtgctt tttcagatag ttgttcctaa
      541 gtttactaaa ctgcaaaaat tagtagataa actaaattta gttacccgag aaattcttac
      601 tggcttaagg gttattaagg cttttggcaa ggaagactac gaaactcaga aatttgacaa
      661 agttaatcaa gatttaacac ggcttcatct tttggttaac cgcttaatgg ttattattca
      721 accagtcatg atgcttatta tgaattttat gatgattgct attgtttggc tgggggcaca
      781 tcatattggg gctggtgatt tgcaggttgg cagtatgttg gctttcttac aatattccat
      841 gcaggcagta tcggcctttt taatgatttc tattgttttt attaatctgc ctcgtgctgc
      901 tgtttcggct aataggattg ctgaagtact ggaaactgaa attcaaatta aagatccaac
      961 taacccagtg agggctcata gacggggtgg caaggtagaa tttaaaaatg ttacctttac
     1021 ttatccggga gccgctgagc ctgttcttca taatatttct tttgtggctt tgcctggcga
     1081 aacaacagct tttgtcggta gtacgggcag cggtaaatca actattatta accttattcc
     1141 tcgtttttat gaggtgactg aggggcaggt tttagttgat gatgtagatg tgcgggcaat
     1201 gagacaaaaa gatttgcggg cgcgaattgg ttatgtatcg caaaaagcta tgcttttttc
     1261 tggtactatt agagataata tcacttacgg caatcctaat gctacccagg aagagatttt
     1321 gcgagccgct gctacggctc aagctttaga atttattaaa gagttagagg gaaaattcga
     1381 tagctcgatt gctcaggctg gcactaattt ttctggcggt cagaaacaac gtttagctat
     1441 tgccagggct ttagccaaga accctgaact ttatcttttt gatgatagtt tttcggccct
     1501 ggattttcag accgataaag ccttaaggac tgcattaaaa gaagaagctc aaggcaagac
     1561 cgttttaatt gttacccagc gtattgcgac cattatgaat gctgacaaaa ttgtagtaat
     1621 agacaaagga gagatagtgg ggcaggggac tcatgaaact ttactcaaga cttgcaaggt
     1681 ttatcaagaa attgcggctt cacagcttgc cccggaagaa ttagctcgag ttctgaaata
     1741 attattatgt ctaaagataa agaaaaatta aataatccta ccacttttac tttacctccc
     1801 agaggaccac gggttggtgg tttaggttta ggtcgtcggc atggtggagg gatacggggc
     1861 ctggtggaaa aacccaagga ttttaagggt accatgcgca agcttataaa atatctaaag
     1921 ccttattacg tctccattat ggtggttcta attttagcca tggctagcac tgtttttact
     1981 attattagcc ctaagatatt gggagatatt actaatgaaa ttgttgccgg cattaagaat
     2041 ataacccagg ggggtattga tttttccgct attcttcact taataacaat tttggttggt
     2101 ctttatttga ttggcgcctt atgtggctat cttcagggtt ggattatgac gggggttgcc
     2161 cagaaagtta cttataatct aagaaaaagt attgctttaa aaattaatcg gctgccgcta
     2221 cgctattttg atacccaaag ccatggagat atcttaagcc gggtaaccaa tgatgttgat
     2281 accgttagcc atagttttaa tcagagttta acgcaagtgg tgaccgctgt gactactatt
     2341 gtcggtattc tcataatgat gattattatt agttggcagt tgaccttagt cgccgtgatt
     2401 gttttgccgc ttggcttttt attggcgcga tttattatca aaaaatcaca aaagtatttt
     2461 tttaatcatc aagtatcgtt aggggagatt aatggtcata tagaagaaat gttttctggt
     2521 catactattg ttaaagcttt taatggagag aagaaatcta tcagcacttt tcgtaaaatt
     2581 aataataaac tttataataa cgcctggaag tctcaatttt tttctggtct tctacatccc
     2641 ataatgaatt ttttggataa tgctggattt gttgccgtgt cggtgctcgg cggatggttc
     2701 gctattcgcg gcaaaatgac tattggtagt attcaggctt ttattcaata ccttaaccaa
     2761 tttaatcgtc ctattattca gacggctagc attgctaatg tttttcaatc tacggctgca
     2821 gctgccgaaa gagtctttga atttttagaa gaagaggaag aacttccaga tccggctcaa
     2881 cccaaagagc ttaagacagt aaaaggtgct gttgattttg tgaatgtggt gtttggctat
     2941 actcccgaaa agataattat taataatttc agtgccacta ttaaaccagg gcaaaaaata
     3001 gcgattgtcg gaccaactgg tgctggcaag accactatag ttaatttgtt aatgcgtttt
     3061 tatgaggttg atagtggtgc tattaaaatt gatggtgtag atattaaaga tttaaggagg
     3121 tcagatttaa gaaaactttt tgggatggta cttcaggata cttggctttt tagcggcacg
     3181 attgaagaaa atattgccta tggaaaagac aatgtttctc acgaagaagt agttaaagcg
     3241 gcgcaagcag cttatgctga ccactttatt catactttgc ctggagatta ccagatggag
     3301 attagcgagg aagcagataa catttctcaa ggcgagaaac aacttttaac tatagcgcgg
     3361 gctattttag ctaatgcccc catgcttatt ttagatgaag ctaccagctc agtggatacc
     3421 cgtactgaag ctttgattca ggcggccatg gataaaatga tggaaggccg gactagcttt
     3481 attattgctc atcggctgtc aactattcgc aatgctgatt taattttagt gatgcgtgat
     3541 ggccatattg tggagcaggg gacacataaa caattgttgg ccca
//
LOCUS       DAOOIS010000032         3561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_20351_length_3561_cov_76.235026, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000032 DAOOIS010000000
VERSION     DAOOIS010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20351_length_3561_cov_76.235026"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..2994
                     /gene="uvrA"
                     /locus_tag="PK960_02665"
     CDS             97..2994
                     /gene="uvrA"
                     /locus_tag="PK960_02665"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPD08096.1"
                     /translation="MPSKHNHDWLKIRGARVNNLQNINVDIPRNQLVVITGLSGSGKS
                     SLAFDTIYAEGQRRYVESLSAYARQFLGLMNKPDVDSIEGLSPAISIDQKAVSHNPRS
                     TVGTITEIYDYLRLLYARLGQPHCPNCGDPIKANTLDEIVEQIKKQSAGKKIAILAPL
                     VRDKKGEHKNVLMAIGRAGFSRIRLDNYFYDLAEAQGLTLDKQKKHTIEIVIDRLTIT
                     NNEDSISRLTEAVDKALDLANGLVGTIELTEEKAQEKLYSQQFACPRCNISLPEMEPR
                     NFSFNSPHGACPYCSGLGTKLEIVPELVFNKNLTIAQGAIRPWSHYSYSSQLWLTRVV
                     GAVANAHGFDLNTPLNKLTPEQLAVLENGTGDNVYILPSTNGYGTVRAKFEGVLNNLY
                     ERYQQTDSDFIRHEIEKYMRTIPCPVCHGARLKPEFLAVTVAGRSIYEVGEQTIAQNY
                     DFFQKLNTSHELTLKQKQVGKLIFKEIINRLQFLLNVGLDYLTLNRPAATLSNGEAQR
                     IRLATQIGSALTGVIYVLDEPSIGLHPKDNDKLINTLKELRDLGNSVLVVEHDEATML
                     AADQIIDIGPGAGKHGGRVVAQGKAKDIMANPNSLTGQYLSGKKSIPLPTKYRPGNGQ
                     VLTIIGARQNNLQNLTVNFPLGKFIAVTGVSGSGKSTLITETLGRALKKKFYQAADEP
                     GAYDRLEGVGNINKIIEIDQSPIGRTPRSNPATYTGAFTPIRELFASLPEAKLRGYKA
                     GRFSFNVVGGRCEACHGDGLVKIEMQFLPDVYVTCEVCHGQRYNQEALEIHYKDKNIY
                     EVLEMTVEEALAFFANQPAIKDKLEALDQVGLGYIKLGQAATTLSGGEAQRVKLATEL
                     ARKGTGRTLYILDEPTTGLHFADIQRLLDVLHRLVDKGNTVIVIEHNLDIIKTADWII
                     DLGPLGGERGGQIVAEGTPQDIAASPQSFTGEYLKAIFNKASSLDTQPKIA"
     gene            3050..3412
                     /locus_tag="PK960_02670"
     CDS             3050..3412
                     /locus_tag="PK960_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPD08097.1"
                     /translation="MNETQAPTLRDLKPGMTVRIYQKIKELNPKGEEKERIQYFEGLV
                     IARKHGQEAGGTVTVRKISNGVGVEKIFPINLPTITKVEIKKQAIVRRAKLTFIKNYK
                     KRLRDINEKKTATPKKTD"
     gene            3413..3496
                     /locus_tag="PK960_02675"
     tRNA            3413..3496
                     /locus_tag="PK960_02675"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3447..3449,aa:Leu,seq:gag)
BASE COUNT         1108 a          778 c          715 g          960 t
ORIGIN      
        1 aaatcttaat aaattgacaa caaggttagg agctttgact aaccgctttt ttttagttat
       61 aataatcatt taattaataa attacgtatt ttacctatgc cttctaaaca taaccatgat
      121 tggttaaaaa ttcggggagc tcgtgttaat aatttacaaa atattaatgt ggatattccc
      181 cgaaaccaat tagtggtcat tactggactt tccggctcgg gcaaatcctc acttgccttt
      241 gatactattt atgccgaagg ccaacgtcgt tatgtagaat ctctgtcggc ctatgcccgg
      301 caatttttag gcctcatgaa taagccagac gtagattcaa ttgaaggatt gtctccggct
      361 atttctattg accaaaaagc tgtctcccac aatccccgct ccaccgttgg gactattact
      421 gaaatctatg actatttaag attactctac gcgcgtctag gtcagcccca ttgtcccaat
      481 tgcggcgacc ccattaaggc caatacccta gacgaaattg tcgagcaaat taaaaaacaa
      541 tcggccggta aaaagattgc catcttagcc ccactggtgc gcgacaaaaa aggcgaacac
      601 aagaatgtcc taatggctat tggacgagct ggctttagcc gaatacgtct agataattat
      661 ttctatgatt tagctgaagc ccaaggtcta accttagata aacaaaaaaa acacaccatt
      721 gaaatagtga tagaccgctt aacgattact aataacgaag atagcataag tcgtcttact
      781 gaggccgtag ataaggctct agatttagcc aacggcctag taggcactat tgagcttact
      841 gaagaaaaag cccaggaaaa actatattcg caacaatttg cctgcccccg ctgtaatatt
      901 agtttaccag aaatggagcc ccgtaatttt tcttttaatt caccgcatgg cgcttgccct
      961 tactgttcag gacttggcac taaattagaa attgtcccgg agctagtatt taataaaaat
     1021 ttaaccattg ctcagggtgc cattagacca tggtctcatt atagttattc tagccaatta
     1081 tggcttacca gggtggtggg tgctgtagct aatgcccatg gctttgacct taatactccc
     1141 ttaaataaat taacgcccga acaattagct gttctagaaa atggtactgg cgataatgtc
     1201 tatattctcc ccagtactaa cggttacggc actgtcaggg ctaaatttga aggagtttta
     1261 aataatcttt atgagcgtta tcagcaaaca gacagcgact tcatccgcca tgagattgaa
     1321 aaatatatgc gcactattcc ttgccctgtt tgtcatggcg cgcgtctcaa accggaattt
     1381 ttagctgtta ctgttgccgg gcgatctatt tatgaagtgg gtgaacaaac tattgctcaa
     1441 aattatgact ttttccagaa attaaatacc agccatgagc ttacgctgaa acaaaaacaa
     1501 gtcggcaaac ttatcttcaa agaaattatt aaccgactac aatttttgct caatgttggt
     1561 ctagattatt taactctcaa tcggccagct gccaccctct ctaatggtga ggcccaacgt
     1621 attagactag ctacgcaaat tggctcggcg ctaactggtg taatctatgt cttagatgaa
     1681 ccctcaattg gcctacatcc taaagataat gataaactca ttaacacctt aaaagaatta
     1741 cgtgacttgg gcaatagcgt cctggtagta gaacatgatg aagccaccat gcttgccgct
     1801 gatcaaatta ttgacattgg ccctggcgct ggtaaacatg gcggccgagt agtggctcaa
     1861 ggcaaggcta aagatattat ggccaatcct aattccctga ctggccaata tctatctgga
     1921 aaaaaatcca tccccctacc aactaaatac cggcccggca atggacaggt tcttacgatt
     1981 atcggggcta gacaaaataa tctacaaaac ttaacagtta attttcctct aggcaaattc
     2041 atcgccgtaa ccggtgtctc cgggtcaggt aaatctaccc ttattactga aactttaggt
     2101 cgagctttaa agaaaaaatt ttatcaagcc gccgatgaac ccggcgctta tgaccgcctg
     2161 gagggagtag gaaatattaa taaaataatt gaaattgacc aatcccccat tggccgcact
     2221 cctcgctcta accctgctac ctataccggc gcctttactc ccattcgcga actctttgct
     2281 agcctgccgg aagctaaatt aagaggctat aaagctggac gtttctcttt taatgtggtc
     2341 ggcggacgtt gtgaagcttg ccatggcgat ggcttagtga aaattgaaat gcaatttctc
     2401 cctgatgttt atgttacctg tgaagtctgc cacggccaaa gatataatca agaagcactg
     2461 gagattcatt ataaagataa aaatatttat gaagtgctag aaatgactgt tgaggaagca
     2521 ctagcctttt ttgctaacca gccggctatt aaagataagc tagaagctct agatcaggtc
     2581 ggactgggct atattaaatt aggccaagca gctaccaccc tctccggtgg cgaagcacag
     2641 cgagtaaaat tagccacaga attagcccga aagggcaccg gtcgtacttt gtatattctc
     2701 gatgagccaa ctactggtct acattttgcc gatatccaaa gactattaga tgtcctgcat
     2761 cgtctagtag acaaaggcaa taccgtcatt gtcattgaac ataatttaga tattattaaa
     2821 actgctgact ggattattga cttagggcca ctaggtggcg aacgaggcgg tcaaatagta
     2881 gccgaaggca cgccgcaaga tattgctgct agcccccaga gctttactgg cgaatatcta
     2941 aaagctatct ttaataaggc aagcagcctt gacactcaac ctaaaattgc ttaagctaat
     3001 aaagcttaaa ctacaagcgc tattttttat tagaaaagaa gataaactta tgaatgaaac
     3061 gcaagctcct acattacgtg accttaagcc aggcatgacg gtccgtattt accaaaagat
     3121 taaagaactt aatcctaaag gtgaggaaaa agaacgaatc caatattttg aaggtctagt
     3181 tattgcccgt aaacatggtc aggaagccgg aggtacagtt actgtccgca aaatttctaa
     3241 tggagttgga gtcgaaaaaa tcttccccat taatctacca accattacta aggtagaaat
     3301 aaaaaaacaa gccatcgtcc gacgggctaa attaactttc attaaaaact acaaaaagag
     3361 gctcagagat attaacgaaa aaaagacggc tacccccaaa aagactgact aagccgaggt
     3421 ggtggaaatg gtagacacgc aagcttgagg tgcttgtgaa ggaaacttcg tgcaggttca
     3481 agtcctgtcc tcggcacaac tagggcgcat agctcagttg gttagagcgc ttcgtttaca
     3541 ccgaagaggt ccaaggttca a
//
LOCUS       DAOOIS010000033         3314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_22285_length_3314_cov_38.437864, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000033 DAOOIS010000000
VERSION     DAOOIS010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22285_length_3314_cov_38.437864"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..625
                     /locus_tag="PK960_02680"
     CDS             110..625
                     /locus_tag="PK960_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08098.1"
                     /translation="MRRPYFFIALMIITLAIAACSSNTARVKVGLDANRQQILAVDAK
                     GALPAEDWVQLRGDQDWTRLQAYYLQKAQEATTPEASQAWLDLANQVMGQAISGTTLV
                     RLVNASDYRIVVLDGPFAGIILNPGEASLVETRVPVGTLVFTVGWGNNQRATIIRQIS
                     AGQKAVVIVNK"
     gene            complement(664..1062)
                     /locus_tag="PK960_02685"
     CDS             complement(664..1062)
                     /locus_tag="PK960_02685"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08099.1"
                     /translation="MKKKLLILSIIIITGQLLTSPTAQAQQPNEDVIKHLQIVAADSN
                     VYSPYTNEDTLLNNVASIIKVVLGLLGTIFVILMIYAGILWMTAGGNDTQVKKAQNII
                     QRAVIGLIIVVLAYAITYFIFKELGGLGPA"
     gene            complement(1067..1525)
                     /locus_tag="PK960_02690"
     CDS             complement(1067..1525)
                     /locus_tag="PK960_02690"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HPD08100.1"
                     /translation="MKYHISKIISQFIIQPIAIVMIIIAPIHTQYVEASGNLNDAFSN
                     VMKETGEEAGYSYNSTTTSPLPIISKVINVVFGLTGVIVFVLIIYGGILWMTAGGNDE
                     QVKKAQKIIQRAVIGLIIVVLAYAITYFILKAIFANQPAPIFHDYIKIKK"
     gene            complement(1522..1935)
                     /locus_tag="PK960_02695"
     CDS             complement(1522..1935)
                     /locus_tag="PK960_02695"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08101.1"
                     /translation="MKKRKKFFVLSVFTLIVFTLYVPLNCYSKNNTANAAAIDLFSNS
                     AKTTANKMGYNPLTSELTILMQIGLIVNVVFGLTGVIVFVLIIYGGILWMTAGGNDEQ
                     VKKAQKIIQRAVIGLIIVVLAYAITYFILKAILVK"
     gene            complement(1942..2337)
                     /locus_tag="PK960_02700"
     CDS             complement(1942..2337)
                     /locus_tag="PK960_02700"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08102.1"
                     /translation="MGKKLSIIKNSYKLFWIFILGVALIQNIIIPNARAASKSNFGLD
                     ITASHAGLVTDTTLQAKVGQIVGSVLALIGVLFFVLMIYGGITWMTAGGNETTVTNAK
                     KTITAAVIGLVIVISAYAITLFLGEGLAG"
     gene            complement(2442..2831)
                     /locus_tag="PK960_02705"
     CDS             complement(2442..2831)
                     /locus_tag="PK960_02705"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08103.1"
                     /translation="MKKWLIGLMSLVWACSFISLSPAPVRAIDVWGTGTIDAYTNDQN
                     GVGFSETDPRQVVARIVKIALGFLGTIAVVLIIVAGFQWMTAAGNEEKIGKAKKLMSA
                     AVIGLVIVLMAFALSSFIINAVITAVS"
     gene            complement(2875..3264)
                     /locus_tag="PK960_02710"
     CDS             complement(2875..3264)
                     /locus_tag="PK960_02710"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08104.1"
                     /translation="MKLSKKILFICLLLQCLIINPLTLQPAKAQVDLWGNNVGTNAFN
                     NIGFKEKDPREIVANIIKIALGFLGTIAVVLILYAGFLWMTAGGKPDTVSKAQKIMAA
                     AVIGLVIILASYGLTLFITRSLSNAVG"
BASE COUNT         1070 a          627 c          607 g         1010 t
ORIGIN      
        1 ttatctcaga aattgcagtt ttgtcaatta ttgacaaaat ttactttatg tgttatacta
       61 ttactatagt acctaaaaaa tataaataaa aataaaaagg aggtgtcaaa tgagacgccc
      121 atactttttt atcgccctaa tgattattac tctggctatt gctgcttgca gcagtaatac
      181 ggccagggta aaagttggcc tggacgccaa ccgccaacag attttggccg ttgatgcgaa
      241 aggcgccttg cccgctgaag actgggtcca gttgagaggt gaccaagact ggacaaggtt
      301 gcaggcttat tatttacaga aggcccagga agctaccact cctgaggcta gtcaagcctg
      361 gcttgactta gctaatcagg taatgggcca agctatctcc ggcaccacct tggtcaggtt
      421 ggtaaatgcc agtgattatc ggatcgtcgt tttggatggt ccattcgctg gaataatttt
      481 gaaccccggg gaagccagcc tcgttgagac tagggtacct gtggggacct tagtttttac
      541 cgttggctgg ggaaacaacc aacgagcaac aataatccgg cagatttctg ccggacaaaa
      601 ggcggtggtt attgttaaca aataaccact gtctatcccc gctagtaata gcggggtttt
      661 attttaggct ggccctaatc ctccgagttc cttaaatatg aagtaagtaa tagcataggc
      721 taggacaaca ataatcaggc caataaccgc tcgttgaata atgttttgtg cttttttaac
      781 ttgtgtatca ttaccgccag cggtcatcca cagaatgcca gcataaatca ttaagataac
      841 aaaaatggta cccagcaagc caaggactac ttttattatg ctagccacat tattcaataa
      901 agtatcttca ttagtatatg ggctatatac attactgtcc gcagccacta tttgtaaatg
      961 tttaattacg tcttcgtttg gttgttgagc ttgagcagtt ggactagtaa gtaattgccc
     1021 tgtaattatt ataattgata atattaataa tttttttttc ataagtttat ttttttattt
     1081 ttatgtagtc atgaaaaatt ggtgcgggtt gattagcaaa gattgcttta agtatgaaat
     1141 aagtaatggc ataggctagg acaacaataa tcaggccaat aaccgctcgc tggataatct
     1201 tttgtgcttt tttaacctgt tcatcattac caccagcggt catccataag ataccaccat
     1261 aaataattaa gacaaagaca atgacaccag ttaggccgaa gacaacatta atgactttgc
     1321 taattatagg taatgggcta gttgtagtgc tgttatagct atagccagcc tcttctcctg
     1381 tctctttcat tacattagaa aaggcatcat taagattacc acttgcttct acatattgtg
     1441 tatgtatagg agcaataatt atcataacta tcgcaatagg ttgaataata aattggctga
     1501 ttattttaga aatgtggtat ttcattttac taagattgct ttaagtatga aataagtaat
     1561 agcataggct aggacaacaa taatcaggcc aataaccgct cgctggataa tcttttgtgc
     1621 ttttttaacc tgttcatcat taccaccagc ggtcatccat aagataccac cataaataat
     1681 taagacaaag acaatgacac cagttaggcc gaagacaaca ttaacaataa gacctatctg
     1741 cattaatata gttaattcgc tagttagggg gttataaccc attttattag cagttgtctt
     1801 ggcactatta gagaaaagat caattgctgc agcattagca gtattatttt tagaataaca
     1861 atttaaaggc acgtagagtg tgaagacgat gagggtaaaa acacttaata caaaaaattt
     1921 ttttcttttt ttcataataa attatcccgc caatccttct cccaagaaaa gagttattgc
     1981 ataggctgaa attactatga ctagtccaat aactgcagca gtaatagttt ttttagcgtt
     2041 ggtgacagta gtttcattac cgccagctgt catccaggta ataccaccat agatcattag
     2101 aacaaagaaa agcactccaa ttaaagctaa aaccgatcca actatttgtc caaccttggc
     2161 ctggagggta gtatccgtta caagaccagc atggctagcc gttatgtcta ggccaaaatt
     2221 agactttgag gctgccctag cattggggat aataatattt tgaattaagg ccacccctaa
     2281 aataaaaatc cagaataatt tataagaatt tttgatgata cttaattttt tgcccatgag
     2341 aaatcattta aaaattttgt ctcagcacaa ttagtaatta ggctgaagca aaatttgcca
     2401 aaatattttg gcaaatttga gctgtagcta atatagcaaa attagctaac ggcagtaata
     2461 acagcattaa taataaagct gcttagagca aaagccatta gaacaataac caaaccaatg
     2521 accgctgctg acattaattt tttggcttta ccaatttttt cttcattacc agccgctgtc
     2581 atccactgaa agccagctac aataattaat actactgcaa tagttcccaa gaaacccaag
     2641 gctattttta caattcgagc tactacttgc cgtggatcgg tttcagaaaa tcccacacca
     2701 ttttgatcat tagtataggc atctatggtg ccagttcccc acacgtcaat tgctctaact
     2761 ggcgcagggg ataaagatat aaagctgcag gcccatacta acgacataag gccaattaac
     2821 cattttttca taattttgtt attaagttat aaatgaatta atttttatta aattttatcc
     2881 aacggcatta cttaatgatc tggttataaa caaggtcaat ccataggaag ctagaatgat
     2941 aactaggcca atgacggctg ccgccataat tttttgggct ttgctgaccg tatctggttt
     3001 accacccgct gtcatccaca agaagccagc atagagaatc aatactactg caatagttcc
     3061 caagaaaccc aaggctattt taataatatt ggccactatt tcccggggat ctttttcttt
     3121 aaagccgata ttgttgaaag cgttagtgcc cacattgttg ccccataaat caacctgagc
     3181 tttagctggc tgtaaagtta aaggattaat aattaaacat tgcaggagta aacaaataaa
     3241 gagaattttt ttagatagtt tcataaaaat gttattaaga cacaaattta gattaagatg
     3301 gagctattat aaca
//
LOCUS       DAOOIS010000034         3193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_23364_length_3193_cov_46.785214, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000034 DAOOIS010000000
VERSION     DAOOIS010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23364_length_3193_cov_46.785214"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1598
                     /locus_tag="PK960_02715"
     CDS             <1..1598
                     /locus_tag="PK960_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08105.1"
                     /translation="DTSDQPKIKVSSVISRMAFFYEKIRNVVEYKEEYLLRKEAIKRI
                     LRRKTVIEGDTKECQAAEISEHLLTELIRGGYLPNNQLPLSLIDEVAVIIKKYLVIKN
                     IAAPSISKEISKDLTKRDLREIRDEIAKHNQLVDWLLAVAASEIEARLSQDPVLNQAI
                     KNLYEYLSKNIQLADQQELAKDLPIQIYLSINRNFAKLDDDMLTFILFKYYIADWQNL
                     PENLWPTVAKNIEKIRNVVNSQLRHPLAEPLDKIISRYAVAVSVSLELLRQDPVGIYT
                     SFKTDPQAFSRQIKKICETKYTKAKKLLWRAAVRSIIYIFITKGVLVILLEIPANKIF
                     GAEIEPLALLFNILFPPTLLFFSVLVTKVPDENNTEKIIKLVNELSFEEYKRSEPIIL
                     RVSQKKTSVLRKIFNFFYGLAFIITFGAIIWLLTIFHFTWVSILIFLFFLVFVSFFII
                     RIKKVTNELKITEEKENLWRTIFDFFSLPVLSVGKYLSEKFSKINVFVFFLDFIIETP
                     FKFLVNMVEDWTNYVNERKNQIG"
     gene            1603..>3193
                     /locus_tag="PK960_02720"
     CDS             1603..>3193
                     /locus_tag="PK960_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPD08106.1"
                     /translation="MTEENDNLSIEDLLDSKSLQEKDSPAAQLARREQELREQAIEND
                     VAAQAAQLGLSYIDLTNFPISPEAIVLLSREEAENLQAICFYYVGDNIRIGTTKPSDR
                     LTRKIEELKNQYHVKSKLYLISPRSLGYGLRVYDTLPKIKRLEGVEISPADLVRYQEE
                     LSDFKSLNERLKEGNISQIMTLIIAAALKTESSDVHIEAEENEVAIRMRVDGVLQHVA
                     SLPKSDWKRVLARLKVVARVKINVENKPQDGRFDIFLPKEKIAVRASFLPTAYGESVV
                     LKLLRPESAILPLDSLGMRPAVQAILEQQMAKPNGLILTTGPTGSGKTTTLYAILNKL
                     NSPDVKIITLEDPIEYELEGINQSQVDESRGYTFANGLKSILRQDPNIIMVGEIRDAD
                     TANIAIQASLTGHLVFSTLHTNDAAGVLPRLIDLGVQPYQLAPAINAVIGQRLVRKLC
                     PYCRQVHEPTEEERKQINKILAVISPKANIDVPKELPVFYKAGPGCPHCSGFGYKGRV
                     GIFEIFTMTEPMKVLLNEGAPS"
BASE COUNT         1020 a          519 c          639 g         1015 t
ORIGIN      
        1 aagatacatc agatcagcct aaaattaagg tttctagcgt tatttcccgc atggcctttt
       61 tttatgaaaa aatccgtaat gtggtggaat ataaggaaga atatttgctc cgtaaagagg
      121 caattaaacg aattttacga cgcaaaaccg taattgaagg agatactaaa gagtgccaag
      181 cggcagaaat tagtgaacat ttattaacag aattaattcg tggtggttat ttgccaaata
      241 atcaactacc cctgagcctt attgatgaag tagcagttat tattaaaaaa tatttggtaa
      301 ttaaaaatat tgctgcccct agtataagta aagaaataag taaggattta accaaaagag
      361 atttaaggga gattagagac gaaatagcta agcataatca gttagtggat tggttactgg
      421 cagtagcagc tagcgaaatt gaagctcgtc ttagccaaga ccctgtatta aatcaagcta
      481 ttaaaaatct ctatgaatat ctatctaaaa atattcaatt agctgatcag caagagctag
      541 ctaaggattt gccaattcaa atttatcttt ccattaaccg aaattttgct aagttagatg
      601 acgacatgct gacttttatt ttatttaaat actatattgc agattggcaa aatttacctg
      661 agaatctctg gccaacggta gctaaaaata ttgagaaaat tcgcaatgtt gttaatagcc
      721 aattaagaca cccgcttgct gagccacttg ataaaattat tagccgttat gccgtagcgg
      781 tttctgtttc attagagtta ttacgccaag atccggtagg gatttatacc agctttaaaa
      841 ctgatcccca ggccttttct agacaaatta agaaaatttg tgagacaaag tatactaaag
      901 ctaaaaaatt attatggcga gccgctgtcc gtagtattat ctatattttt attactaaag
      961 gtgttttagt tattcttcta gagattccag ctaataagat atttggcgca gagattgaac
     1021 cgttggccct attatttaat attctttttc ctccaacttt gttgttcttt tcagttttag
     1081 ttactaaagt acccgacgaa aataatacag agaagattat taaactagtg aatgaactgt
     1141 cttttgaaga atataagcgg tcagaaccaa taattttaag ggtatctcag aaaaaaacta
     1201 gtgttttgag gaagattttt aatttcttct atggattagc ctttattata acctttgggg
     1261 ccattatctg gttactgact atatttcatt ttacctgggt tagtatatta attttcttat
     1321 ttttcttggt atttgttagt ttttttatca ttcgaatcaa gaaggttacc aatgagctta
     1381 aaattactga agaaaaagaa aatttatggc gcactatttt tgactttttt agcttaccag
     1441 ttctttcagt tggtaaatac ttaagtgaaa aatttagtaa aattaacgtt tttgtttttt
     1501 tcctagattt tataattgag actccgttta agtttttagt aaatatggtt gaggattgga
     1561 ctaactatgt taatgaacgt aaaaaccaaa taggttaaat ctatgacaga agaaaatgac
     1621 aatttatcca ttgaggattt attagatagt aaatcactac aagaaaaaga ttcccctgct
     1681 gcgcaattgg cacgtcggga acaagaatta cgcgaacagg ccatcgaaaa tgatgtagcg
     1741 gctcaagcag ctcaattggg gttatcatat attgatttga ctaattttcc cattagtcct
     1801 gaggcaattg ttttattaag tcgagaagag gctgaaaact tgcaggctat ttgtttttac
     1861 tatgttggag acaatattag aattggcaca accaaaccaa gtgatcgcct aacccgcaag
     1921 atagaagaat taaaaaatca atatcatgtt aaaagcaagc tttatttgat ttccccccgt
     1981 agtttagggt atggccttag ggtttatgac actctaccca agattaaacg tttagagggt
     2041 gtagaaatta gtccagctga tttagtgcgc tatcaagaag aattgagcga ttttaagtca
     2101 cttaatgaga ggttaaaaga aggtaatata agtcaaatta tgactttaat cattgctgcc
     2161 gctctaaaaa cagagagtag tgatgtccat attgaggccg aagaaaatga ggtggcaatt
     2221 agaatgagag tagacggagt tttacaacac gtagcctctc ttcctaaatc tgattggaag
     2281 agagtcctag cccgtcttaa agttgtggcc agggtgaaga taaatgtaga aaataagcca
     2341 caggatggcc gttttgatat ttttctccct aaagaaaaaa tagctgttag ggcttcattt
     2401 ttaccgacag cttatggtga aagtgttgtt ttaaaattac tgcggcctga gtcagctatt
     2461 ttgcctctag atagtttagg gatgcggcca gcagtccagg ccatattaga acagcagatg
     2521 gctaaaccta atggtcttat tttaactact ggtcccactg gatcaggcaa gacgaccacc
     2581 ctatatgcta tattgaataa attaaactct ccggacgtta agattattac cctggaggac
     2641 cccattgagt atgagctgga gggaattaac caaagtcaag ttgatgaaag ccgaggctac
     2701 acttttgcta atggtttaaa gtcaatttta cggcaggatc ctaatattat tatggtcggg
     2761 gagattcgtg atgctgatac tgcaaatatt gccattcagg ctagtctgac tggtcatcta
     2821 gtattttcta ctcttcatac taatgatgct gctggtgtac tgccccggtt aattgatttg
     2881 ggggtgcagc cctatcaact agcgccagct attaatgctg ttattggcca gcgtttagtt
     2941 cgcaaattat gtccctactg tcgacaggtc catgaaccaa ccgaagaaga acggaagcaa
     3001 attaataaaa ttttagcagt tatttcgcct aaggctaata tagatgtgcc taaagaatta
     3061 ccagtattct ataaagcagg gccaggctgc ccgcattgtt cgggttttgg ttataagggg
     3121 cgggttggta tttttgaaat ttttactatg accgagccga tgaaggtttt gcttaacgaa
     3181 ggagcgcctt cgt
//
LOCUS       DAOOIS010000035         3105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_24219_length_3105_cov_35.313770, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000035 DAOOIS010000000
VERSION     DAOOIS010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24219_length_3105_cov_35.313770"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2093
                     /locus_tag="PK960_02725"
     CDS             <1..2093
                     /locus_tag="PK960_02725"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPD08107.1"
                     /translation="DCGQIRLREPIRVQIDYLDNKLIETTVGRIIFNNILPEKLRFIN
                     DIVVSTQIKALVKDCLRCYGREVTIDFLDELKRESFKFITLSGLSWSIGDLPTFPIRE
                     ELIKQAEKEADEVQQQYEDGLLTDSERYGKIIQLWTSVKDKITTICMENLDKDGPVYS
                     MIKSGARGSWAQLTQIIGMKGLVTAPNGEIIELPIKGNFKKGFDVLEYFISTHGVRKG
                     LSDIALRTANAGYLTRRLVDVAQDLIIKEDDCGTKKGIIITKEESEAIGRTLVKRITG
                     RYLSADLKNAAGKILLKKDQLVDENIAIELEKEDIPQAEIYSVLTCECQKGLCKKCYG
                     YDLAYNRIVEKGTAVGVMAAQSIGEPGTQLTMRTFHTGGVASQEDITQGLPRVEEIFE
                     ARTPKRRAYIAEVSGVINIEETKKTIDPETNEFIGGGSNKIVKISYEGEEMDKYYFFG
                     DPKAKQPKQVNNKPGDNIKVAVSEGDTVGAGTKLFSLGRKVTKATRPGKIFIGPNYVG
                     VIVTVPKVKDYIIPKNFAIIVHNGQKVKVGDQLTEGSLDLRQLYELKGQTATQKYIVQ
                     EIQRVYSSQGQVLNDKHIEIIARQMFSRIYVTDPGDSDLTIGEIVERALFNRINQELE
                     TNKKQLAKGQVLLLGMTKAALTTSSFLSAASFQETSRVLIEAAVSGKVDYLEGLKENV
                     IIGRPIPAGTGMQE"
     gene            complement(2107..>3105)
                     /locus_tag="PK960_02730"
     CDS             complement(2107..>3105)
                     /locus_tag="PK960_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HPD08108.1"
                     /translation="PELEITLPGLLFSDENKIQSITEGDKTFFYVPWIGEYINWLYNY
                     SIGIIALLALLAIIIGGFYWIIAGGNASRVSEAKSWINAAISGLVLALTSYLLLFTIN
                     SNLVKLPPIKMMAIKTIPLEYEIPSEWQTPDAPISGTSTHGVPLYYQCSKYAKSIYYP
                     TKNGKCANSTLCSSGCGVLATFMAINKSTTTKNLREFTQEAINYGARETFNGKKCNGS
                     TASGLIKLAQSYGLDSESISGDYTTAVKPISDYLDRGCVVVISVGFDDKHSNCKFTNG
                     GHFIVLTGWRDRSKQIVDVNDPFGDRSYPGRGDKTWLSLKDWGGCRLSQKFYVCEK"
BASE COUNT         1010 a          510 c          670 g          915 t
ORIGIN      
        1 atgattgtgg gcaaattaga ctgcgagagc caatcagggt tcagattgat tatttggata
       61 ataaactaat agaaaccacg gtagggcgaa ttatttttaa taatattttg ccagaaaaat
      121 tacgttttat aaatgatatc gtggtcagta ctcagattaa agccttagtt aaagattgct
      181 taaggtgcta tggacgggag gttactattg attttttaga tgaattaaaa cgagaaagtt
      241 ttaaattcat aactttaagt ggtctttcct ggagtattgg tgatttgcca acttttccta
      301 ttcgagaaga attaattaaa caagctgaaa aggaagccga tgaagtgcaa caacagtatg
      361 aagatggtct tttaactgat agtgaacgtt atggcaagat tattcaattg tggaccagcg
      421 tgaaagataa aattactact atctgtatgg agaatttaga caaggatggt cctgtatatt
      481 cgatgattaa atcaggagct cgtggttctt gggctcagct tacgcaaatt attggaatga
      541 aaggcttagt gactgcgcct aatggagaaa ttattgagct cccaattaaa ggtaatttca
      601 aaaagggttt tgacgtttta gagtatttta tttctacgca tggtgtacgt aagggcttat
      661 ccgatatagc cttgcggaca gctaatgccg gctatttgac tcgccggtta gttgacgtag
      721 ctcaagattt aattattaag gaagatgact gcggcactaa aaaaggcatt attattacta
      781 aagaagaaag tgaagctatt ggtcgtacct tagttaaaag gattaccggc cgttatctta
      841 gcgctgattt aaaaaatgca gctggcaaaa ttttattaaa gaaagatcag ttggttgatg
      901 aaaatattgc cattgagctt gaaaaagaag atattcctca ggctgaaatc tattcagttt
      961 taacttgtga gtgtcagaaa ggtctatgta agaaatgtta tggctatgat ttagcctata
     1021 atagaattgt agagaagggg acagcagttg gagtaatggc tgctcaatca attggggagc
     1081 caggcactca gttaacgatg cggacatttc atactggcgg agtagcaagt caagaggata
     1141 ttacccaggg tttacctaga gtagaggaaa tatttgaagc tcgtactcct aagcgtcggg
     1201 cctatattgc cgaagtaagc ggggtaataa atatagaaga gactaaaaaa actatcgacc
     1261 ctgaaactaa tgagtttatt ggtggtggtt ctaacaaaat tgttaagatt agctacgagg
     1321 gagaagaaat ggataaatat tatttttttg gggatcctaa agccaaacag cctaaacagg
     1381 taaacaataa gccaggagat aatattaaag tggctgtctc agagggtgat acagtaggag
     1441 ctgggacaaa attatttagc cttggccgta aggttactaa agcaactcgt ccggggaaga
     1501 tttttattgg tccaaattat gtcggggtta tcgttactgt acctaaggtt aaggactaca
     1561 ttattcctaa aaattttgcc atcatagtcc ataatgggca aaaagtaaaa gtaggtgatc
     1621 aattaactga aggcagtctc gatttaagac agctctatga gcttaaaggt caaacagcta
     1681 ctcaaaagta tatcgtccaa gagattcaga gagtttattc gtcacaaggg caggttctta
     1741 atgataagca tattgaaatt attgctcggc aaatgtttag ccgtatttat gtaactgacc
     1801 ccggagatag tgatctaaca ataggagaga tagtagagcg ggcgctattt aaccgaatta
     1861 atcaagaatt agagactaat aagaagcagc tggctaaggg gcaagtttta cttttgggta
     1921 tgactaaagc cgccctgaca accagtagtt ttttgtccgc cgcttctttt caagaaactt
     1981 cacgagtgct aattgaagca gcggtaagcg gtaaagttga ttatctagaa ggcttaaaag
     2041 agaatgttat tattggtcgc ccaataccag ccgggacagg aatgcaagaa taaaattatt
     2101 atttttctat ttttcacaga catagaattt ttgacttaat cggcaccctc cccaatcctt
     2161 aagagataac caggttttat ctccacgacc aggataggaa cgatccccaa aagggtcatt
     2221 aacatcaact atttgtttag accggtcccg ccagccagtt aagacaataa aatggccgcc
     2281 attagtaaat ttgcaattag aatgtttatc atcaaaacca accgatataa ctactacaca
     2341 accacgatct aggtaatcac ttattggttt gaccgctgtt gtataatcac cactgataga
     2401 ctcagagtcg aggccatagg attgagctaa tttaataagg ccacttgctg tggaaccatt
     2461 acattttttg ccgttaaagg tctcacgtgc tccatagttg atggcttctt gggtaaattc
     2521 ccttaaattt ttagttgtgg tagatttatt aatagccata aacgtagcta gcaccccaca
     2581 gccagaagag cataaagtac tgttagcaca cttaccattt tttgttggat aataaatact
     2641 tttagcatac ttcgagcatt gatagtatag tggtacgccg tgggttgatg ttccggatat
     2701 tggcgcatct ggagtttgcc actccgaggg aatctcatat tctaagggta ttgtttttat
     2761 agccatcatt tttattggcg gcagtttgac taaattacta ttaatagtaa atagtaataa
     2821 ataggaagtt agggctaaaa ctaggccgct aatagcggca ttaatccaac ttttggcttc
     2881 agatacccgg ctagcattac cgccagcaat tatccaataa aaaccgccaa ttattatagc
     2941 cagaagagct aagagggcaa taatgccaat ggaataattg tagagccagt tgatatattc
     3001 acctatccag ggaacataaa aaaaagtttt atcgccctca gtaatagatt gtattttatt
     3061 ttcatcacta aataataatc caggtaaggt aatttctaat tccgg
//
LOCUS       DAOOIS010000036         3071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_24586_length_3071_cov_37.026194, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000036 DAOOIS010000000
VERSION     DAOOIS010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24586_length_3071_cov_37.026194"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1051
                     /locus_tag="PK960_02735"
     CDS             98..1051
                     /locus_tag="PK960_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HPD08109.1"
                     /translation="MTTKEIYNLAINLGIEADLRGRQAVIEHQNYIKHKYRRLSKEDK
                     ANFDLERLTNPYADSRLLVDNGRGDIKKILVGIDMEGPELLAAKWLGDIDLVISHHPE
                     GLGLAGLSEVMDLQADVLAQYGIPINIAESVIRPRIEEVHRGVLPANHNRAVDMANLL
                     SLDYMCLHTPADNLGARYLVDLIAEKESQLKRVSDLLELLSQIPEYAQAIRYKAGPIL
                     FAGHKDSHCGRIAVTEFTGGTSGSKDIYEKMANFGIGTVVGMHMSEEHRREAEKNHIN
                     VVIAGHMASDSLGMNLFLDKLEEQGIDVIPTSGLVRIKRLC"
     gene            1045..1617
                     /gene="ruvA"
                     /locus_tag="PK960_02740"
     CDS             1045..1617
                     /gene="ruvA"
                     /locus_tag="PK960_02740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPD08110.1"
                     /translation="MLAFIKGEVIAKRLGSIIVENNNLGYQIMVGEKIWEEANVGETI
                     KLFLHHHLREDSDELYGFTDLETLSLFELLLSVSGIGPKSALNILSLADIKDVQQAII
                     LGDEVMLTKVSGIGRKTAERVILELKNKIASLPDNLLGNHAGSTNEDDLEALIALGYS
                     APAARRALAKVDRAVTNPAERLKLALKELH"
     gene            1621..2583
                     /locus_tag="PK960_02745"
     CDS             1621..2583
                     /locus_tag="PK960_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPD08111.1"
                     /translation="MWQSIDSREADDFVKRFGESEFLQSSVWLDIQSQFYAVDVLGYY
                     WGEQLVAVARLIRRPLWGKCCYYYSPRGPIVKQTPENLWPQLINDLRLWLRKKQALFW
                     RIEPGSHWVNYDFSSLDLYRGPDIQPSETRILSLNLPIEELLSKMHPKTRYNIRLASK
                     RGVTVRQGSRDDFDSLWSLLLITSQRDNFHLHRREYYLALINSPAIKFLIAEYYGQVL
                     AVGIFAYWGDIAIYLHGASGDEHRELMAPYALQWQAIKLAYDLGIQRYDLFGISSQKW
                     PGVTRFKQGFGGDIVHYPGTFDWPLSRGRYNIYNILRQWRRRKS"
     gene            2591..>3071
                     /locus_tag="PK960_02750"
     CDS             2591..>3071
                     /locus_tag="PK960_02750"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPD08112.1"
                     /translation="MNKFFAQFKKTIYSTYLRHLVPAYHFFVNWLADVYYGHPSRQLI
                     VIGITGTTGKTTSVLLIAEVLRAAGYTVGYTSTAMFSDGQRDWLNDKKMTMVGRFFTQ
                     KMLWQMVHNGCHYAIIETTSQGILQYRHRFINYDLAIFTGLYPEHIEAHGSFDNYRRA
                     "
BASE COUNT          881 a          506 c          715 g          969 t
ORIGIN      
        1 aggagataaa aagttggccg gctaaatatg gggcttggca gaaaaaaaac gaagaagcta
       61 ttattagagg tgaatttata aaataacgta ctggtatatg accactaagg aaatttataa
      121 tttagccatt aatttaggga ttgaagctga cctaaggggt cgtcaagcag tcatagagca
      181 tcagaattat ataaaacata agtatcgccg tttatctaaa gaagacaaag ccaattttga
      241 tctagagaga ttaactaatc cttatgctga cagccggcta ttagtggaca atggacgagg
      301 ggatattaaa aaaattttag tgggtattga tatggaaggc ccagaattgc tagccgctaa
      361 atggctaggc gatattgatt tggtaatctc tcatcatccg gaagggttag ggctggccgg
      421 actttccgag gttatggact tgcaggctga tgttttagcc caatatggca ttcccattaa
      481 tattgccgaa tcagttatta ggccaagaat tgaagaagta catcgaggtg tattaccagc
      541 taatcataat cgggcggtag atatggctaa ccttttatct ttagattata tgtgccttca
      601 cacgccagca gataatttgg gcgctcgtta tttagtagat ttaattgctg aaaaggagtc
      661 acaattaaag cgagtgagtg atttattaga acttttatcg caaatccctg aatatgctca
      721 ggctattaga tataaggcag ggccaatatt atttgcagga cacaaagata gtcattgcgg
      781 tcggattgct gtgactgaat ttactggcgg aacgagcgga tctaaagata tttatgaaaa
      841 aatggctaat tttggcatcg gtacagtggt agggatgcat atgagcgagg agcaccggcg
      901 ggaagcagag aagaatcata taaatgtagt tattgctggc cacatggcta gcgattcgct
      961 aggtatgaat ttatttttag ataaattaga agagcagggg attgatgtta tccccacttc
     1021 aggcttagtt aggattaaac ggctatgcta gcttttatta aaggagaggt tatagctaaa
     1081 cgtttagggt caattattgt agaaaacaat aatcttggct accagattat ggttggcgaa
     1141 aaaatttggg aagaggccaa tgtcggagag actattaaat tatttttgca tcatcattta
     1201 agagaggata gtgatgaact ttatggcttt actgacctag aaactttatc tttatttgag
     1261 cttcttcttt cagtttcagg cattggccct aaatcagcct taaatatttt atcgttggcc
     1321 gatattaaag atgtccaaca ggctattatt ttaggcgatg aggttatgct gaccaaggta
     1381 tctggcattg gacgcaagac agcagaacgg gtcattttag agttgaaaaa taaaattgct
     1441 agtctacccg ataatctttt agggaatcat gctggcagta ctaacgagga tgacctggaa
     1501 gctttgattg ctttaggtta tagtgcccca gcagcgcggc gggccttggc taaagtagat
     1561 agagctgtta ctaatccggc cgaacgcctt aaattagccc taaaagagct acattaattt
     1621 atgtggcagt ctatagattc tagggaagcg gatgattttg taaaacgttt tggtgagtct
     1681 gaatttttac aaagttcagt ttggttagat atccagagtc aattttatgc tgttgatgtt
     1741 ttaggttatt attggggaga gcaattagtg gcagtagctc gcttgattcg gcgaccatta
     1801 tggggaaaat gctgttatta ttattcgcca cgcggtccta ttgttaaaca aacccctgaa
     1861 aatctttggc ctcagcttat taatgatttg aggttatggc ttagaaaaaa gcaagcttta
     1921 ttttggcgga tagaaccagg tagccattgg gtaaattatg atttttcctc tttagatctt
     1981 taccgcggac cagatattca gcccagtgaa acgcgaattt taagtcttaa tcttcctatt
     2041 gaggaattac tctccaagat gcaccctaag acacgttata atatccgttt agctagtaag
     2101 cgtggtgtaa cagtgcggca aggtagccga gacgattttg atagcttatg gtcgcttttg
     2161 cttattacta gccagcgtga caattttcat ttacaccgcc gtgaatatta tttagcgctt
     2221 attaattctc cagccattaa attccttata gctgagtatt atgggcaggt tctggcggtt
     2281 ggcatttttg cctattgggg agatatagct atttatcttc atggggcttc aggagatgag
     2341 catcgtgagc ttatggctcc ctatgcactt cagtggcagg ccattaaatt agcctatgat
     2401 ttaggtattc agcgctatga tttatttggt atcagttctc aaaaatggcc gggagtaacc
     2461 cgttttaagc agggatttgg cggggatata gttcattatc cagggacttt tgattggccg
     2521 ctttctcggg gtcgttataa tatctataat attcttcggc aatggagacg gagaaaaagt
     2581 taataattgt atgaataaat tttttgctca atttaagaaa acaatttata gtacttatct
     2641 gcgacactta gtgccagcct atcatttttt tgttaattgg ttagctgatg tttactatgg
     2701 tcatccgagt cggcagttaa ttgttattgg gattactgga accactggta aaaccaccag
     2761 tgttttactt atagctgaag tattgcgagc cgctggctat acagtgggtt atacttctac
     2821 ggcgatgttt agtgatggcc agcgcgattg gcttaatgat aaaaaaatga ccatggttgg
     2881 gcggtttttt acccaaaaaa tgctgtggca gatggtgcac aacggctgtc attatgctat
     2941 tattgagacc acttctcagg ggatattaca atatcgccat cgttttatta attatgattt
     3001 agctattttt actggccttt atcctgagca cattgaggcc catggttctt ttgataatta
     3061 tcgtcgagcc a
//
LOCUS       DAOOIS010000037         2664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_29879_length_2664_cov_58.823687, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000037 DAOOIS010000000
VERSION     DAOOIS010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29879_length_2664_cov_58.823687"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1075)
                     /locus_tag="PK960_02755"
     CDS             complement(152..1075)
                     /locus_tag="PK960_02755"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HPD08113.1"
                     /translation="MICTRCNRQMPDDSTVCPHCGQPVVPPEQIMKEIKVRRLQRYLF
                     YTIVILIVIVAVAIMVRIYNNNTKLVLEISQVKQSLEGAQGELTAAQSELEQKKQDLA
                     KIQAELAESARKMEAADSQLKEKTTAYQNLLTEKTALEQASEQCRMNLNLADANIYGL
                     IVKLGVGVTNKNLMSIPLADANLGGEDSDGDGLSDTIERSLGSDPNKADTDGDGYDDK
                     SEWLHGYNPLGEGMLPINPQYVNAMKGKILLQIEGDKSAWYVASDGKRYYLGNPGDAY
                     AVMRQNEYWTKDWPGYAPPLMSTSEETMATE"
     gene            1266..2633
                     /locus_tag="PK960_02760"
     CDS             1266..2633
                     /locus_tag="PK960_02760"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPD08114.1"
                     /translation="MTIKGKIRQISLLLGDWLLLYLSLFLTLVIRRQHWPEPNIWHDH
                     LIVFSWIFVLWLLIFYIANLYNLNVLAKGPRFTERVLRAIATAAILSLIIFYLWPQAR
                     ITPKTNLAIFTGIYTLAFLTWRHLWRHNLRHALPRNNVLLIGYDQMAEELWQVLNTAP
                     HLGYNPKLLFDYEPPQQSIASWEIISDPDKLMPAIINDHINTIIIAERYRADNQVNQI
                     LFDLLPLKLTYLTLPTFYEQLTGKVPINAIGQSWFLENLNLVDKRWYLALKRGSDFII
                     AALGFIITLPLWLIIVPLIKMTSHGPIIFKQQRVGRDSKPFIIYKFRTMKVEDNNFQP
                     VGDHDKRITPVGRFLRQTRLDELPQFINILRGEMSFIGPRPERPEIVQELARQIPFYN
                     IRHLIQPGVTGWDQVSGEYHSPSLEDTYKKLQYDLYYLKHCSIYLDLTIILKTIATVL
                     SRGGK"
BASE COUNT          777 a          633 c          437 g          817 t
ORIGIN      
        1 ttattaagtc gcccggtcgg tctagcgtag aattaacgct ccccccttaa acgttattcg
       61 gccaaaccga ccaggcgacc taacaaaaac aatccgcaga cggattgttt agccccctcc
      121 ttaaaaaata taattattaa tcacttacaa actattctgt tgccattgtc tcctcactag
      181 tagacattaa tggcggcgca taacctggcc aatctttagt ccagtattca ttttgccgca
      241 ttaccgcata agcatcacct ggattaccta aataataacg tttaccatca ctagctacat
      301 accaggcaga tttatcaccc tctatctgta ataaaatctt acccttcatg gcattaacat
      361 actgtggatt aatgggcaac attccttctc ctaaaggatt ataaccgtgc aaccattcac
      421 ttttatcgtc atagccatca ccatcagtat ccgccttatt gggatcacta ccaagcgaac
      481 gttcaatggt atcagataaa ccatcaccat cactatcttc gccacccaga ttagcatcag
      541 ccaagggaat gctcattaaa tttttattag ttactcctac acccaactta acaatcaagc
      601 catagatatt ggcatcagct agattcaaat tcatacggca ctgctcactg gcttgctcta
      661 gggccgtctt ttctgttaat aaattctggt acgccgtggt tttttcttta agttgactat
      721 cagccgcttc catcttacga gcactctccg ctaattcagc ctgtatctta gctaaatctt
      781 gcttcttctg ctctaattcg ctttgagccg ctgtcaactc accctgtgct ccctctagac
      841 tttgtttaac ctggctaatt tctaatacca atttggtatt attgttataa atccgcacca
      901 taatcgccac tgcgacaata acaattaaaa tgacgatggt ataaaataaa taacgctgca
      961 gacgacggac tttaatttcc ttcataattt gctcgggagg aactactggc tgaccacagt
     1021 gagggcagac agtactatca tccggcatct ggcgattgca cctagtgcaa atcataaatt
     1081 gggggttaag ctagcttact gccattatta taatctaaat tgacaacttg agcaaatatt
     1141 tttttttggc taacaataaa gcaattttta agatgaaatc taaacgacta aataaatatc
     1201 attttgaggg aacattaaac aaattcttct tgcttttttg tgtatgatga ttataataaa
     1261 attatatgac cataaaaggc aaaattagac aaatcagtct cctcctcggt gattggttat
     1321 tattatatct aagcttattt cttaccttag tgatccgacg ccagcattgg ccagaaccaa
     1381 atatctggca tgaccatcta atagttttta gttggatctt cgtcttgtgg ctcctcattt
     1441 tctatattgc caatttatat aaccttaatg ttctcgccaa aggaccacgc tttaccgagc
     1501 gagtcctcag ggctattgct actgccgcta tcttgtcgct aatcattttt tatctctggc
     1561 cccaagcgcg gattactcct aaaactaatc tggccatttt taccggcatc tatactctgg
     1621 cttttcttac ttggcgtcat ctctggcgtc acaatctacg tcatgcccta ccgcgcaata
     1681 atgttctcct aattggctac gatcaaatgg ctgaagaatt atggcaagta ttaaatactg
     1741 cccctcacct tggctataat cctaagctcc tctttgacta tgagccaccc caacaatcta
     1801 tagctagctg ggaaattatt agtgacccag ataaactaat gccagctatc attaatgatc
     1861 atatcaatac tattattatt gctgaacggt accgggcaga taatcaggta aaccaaattc
     1921 tctttgacct tctgccgctt aaactaacct atctaactct gccaactttc tatgaacaat
     1981 taacgggcaa ggtgccaata aatgccattg gccaatcttg gtttctcgaa aatcttaatt
     2041 tagtagataa acgctggtat ctagccctga aacgaggcag tgactttatc atcgctgctc
     2101 ttggctttat tattacttta ccgctttggc taattattgt cccgctcatt aaaatgacga
     2161 gccacggccc gattattttt aagcaacagc gagtcggtcg agatagtaag cccttcataa
     2221 tttataaatt ccggacgatg aaggtagaag ataataattt ccaaccagtc ggcgaccatg
     2281 ataagcggat tactccggta ggccgcttct taagacaaac cagattagat gaattaccac
     2341 aatttattaa tattttaaga ggcgaaatga gctttattgg accacggcca gagcggccag
     2401 aaatagtcca agaattagcc cgtcaaattc ctttttataa catccgccat ttaattcaac
     2461 cgggtgttac tggctgggac caggtctcgg gtgaatatca ctccccgtcg ctggaagaca
     2521 cctacaaaaa actccaatac gacctctatt atctaaaaca ttgctctatc tatctagact
     2581 taactattat tctaaaaact attgccacag tattatcacg aggtggcaaa taatcatttc
     2641 ttgacaatta tcgtctcaaa atga
//
LOCUS       DAOOIS010000038         2577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_31290_length_2577_cov_53.239889, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000038 DAOOIS010000000
VERSION     DAOOIS010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31290_length_2577_cov_53.239889"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PK960_02765"
     CDS             complement(<1..233)
                     /locus_tag="PK960_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPD08115.1"
                     /translation="MKKILFLIALIFGAIWLGAITYTVKLGDTLEGIAQTYGVGLADL
                     AEVNNIDPPYIIWVGMKLKIPETSQQPQLRAVDT"
     gene            complement(336..1193)
                     /locus_tag="PK960_02770"
     CDS             complement(336..1193)
                     /locus_tag="PK960_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08116.1"
                     /translation="MTYWSIIKKRQAIIWWWLLGFVVVAALFSFTQPLKYSAKSQLLV
                     VPNYQEGADPYQISRTNEYLSTLLSQITYSSSFFEATVKPEYHIDTAYFGDTFKKRLA
                     TWHKTIEVKPVQDYGIISVKVYHPDKDQANKLVQAIDYNLITRANYYHGLGNKVTIKV
                     IDEPVVSSWPVKPNVPLNFGLAIVLGVLFGFIHIYIISASRPQTYAEMALDWPSSEAI
                     KDMARESATITSEGKDHQADNDSGESHVSLDNLVQVPVAAEVTINDDGEDITEQLLHG
                     GNIDNIFAS"
     gene            complement(1325..2254)
                     /locus_tag="PK960_02775"
     CDS             complement(1325..2254)
                     /locus_tag="PK960_02775"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPD08117.1"
                     /translation="MEMGVKDLIIVGAGPAGLTASIYASRYKIEHQVIGLLPGGAMTE
                     AYKICNFPSEVSISGWELAQKIENNARQLGGEIVAGEVLKISGHYPNFQVDLADGHKL
                     ASKTILLASGAKRRHLGLPNEDKFLGKGLAYCATCDGPFFKDKIVGVVGGGNCAATAV
                     IFLSTIAAQVLWFYLGSEPNAVEPGWLEEINKQGNIKLMAQSEIKALLGDSHLNGVTI
                     NRENKTENINLEGLFVEIGSDPNSSLVKQLGGTVNEGGYIVVDGGQATTIPGIWAAGD
                     VTTSSNGLRQIITACSEGAIAADSIFKFLQRQV"
     gene            complement(2258..>2577)
                     /gene="trxA"
                     /locus_tag="PK960_02780"
     CDS             complement(2258..>2577)
                     /gene="trxA"
                     /locus_tag="PK960_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPD08118.1"
                     /translation="ELTPANFATEVLNSKIPVLVDFWAPWCGPCQMMAPILENLAQEL
                     GDKIKIAKVNVDDPDNQPLAVEYQIQSIPNMKLFKNGEEIASFIGVRQQASLRQDILD
                     KLS"
BASE COUNT          804 a          606 c          419 g          748 t
ORIGIN      
        1 gtatcaacag cccttaattg gggctgctgg ctggtctcag gaattttcaa cttcatcccc
       61 acccaaatga tatagggagg gtcaatattg ttaacttcag ccaggtctgc cagacctaca
      121 ccataagttt gggcaatacc ctctaaagta tcacccagct ttacagtata ggttatagcc
      181 cccagccata ttgccccaaa aattagagca attaagaata gaatcttttt catctttgac
      241 tccctcaccc cttttggggt tatatttatt taattgttaa agtgcaccgc tacttgcggt
      301 tccccagcag gccaaagatt gacctactga aaaacttaac tagcaaaaat attatcaatg
      361 ttgccaccgt gcaataattg ttcagtaata tcctcaccat catcatttat ggttacctca
      421 gcggccactg gaacttgaac taaattatct agactaacat gcgactcacc cgaatcatta
      481 tctgcctgat gatccttacc ctcactagtt atagtggctg actctcttgc catatcttta
      541 atagcttctg aactgggcca atctaaagcc atctcggcat aagtctgtgg ccgactagca
      601 ctaataatat aaatatgaat aaagccaaaa agaactccca aaacaatggc taaaccaaaa
      661 ttaagcggta cgtttggttt aactggccag ctagaaacta ccggctcatc tattacttta
      721 atggtaactt tattgccaag cccgtgataa taattggctc tagtaatgag attataatca
      781 atagcttgta ctaatttatt tgcctggtct ttatctggat ggtacacctt aacactaata
      841 atcccataat cttggactgg cttaacctca atggttttat gccaggtagc caaacgtttc
      901 ttaaaagtat caccaaaata agcggtatca atatgatatt cgggcttaac tgttgcctca
      961 aaaaaagagc tagaataggt tatctgagag agcaaagtac tcaaatactc gttagtacga
     1021 gaaatttggt aagggtcagc gccctcttga taattaggca caactaataa ttgactcttg
     1081 gccgaatatt taagtggctg agtaaaacta aacaaagcgg caacaaccac gaaaccaagt
     1141 agccaccacc atataattgc ctgacgtttt ttaattatgg accaataagt catctgttta
     1201 tatttatctt ataaaaaaat aatagatagg ttaaattaac atagccagcc ttatttgtca
     1261 atcacaaaat accccataaa gggggtagca aaaaaataaa cactaaaatg agtaaaaata
     1321 taaactaaac ttgccgttgc aaaaacttaa aaatactatc tgccgctata gcaccctcgc
     1381 tacaagccgt aataatctgc cttaacccat tagaagacgt ggtgacatca ccagctgccc
     1441 agataccggg gatagtagtt gcctgacctc catctaccac aatatatcct ccttcattga
     1501 cggtgccacc caactgcttt actaaactac tattagggtc gctgccaatt tctacaaata
     1561 aaccctctaa attaatattt tcagtcttgt tttctctatt tattgtcacg ccgtttaaat
     1621 gactatctcc caataaggct tttatttcac tctgggccat aagtttaatg ttgccttgtt
     1681 tattaatttc ttctagccaa cctggttcaa ccgcattggg ctcactgcct aaataaaacc
     1741 acagaacttg agccgcaata gtagacaaaa aaataaccgc cgtcgccgca caattacccc
     1801 ctcccaccac accaactatt ttatccttaa aaaatggtcc atcacaagta gcgcaataag
     1861 cgagaccttt gcccaaaaat ttatcttcat taggtaatcc taagtgccgt cgtttagccc
     1921 cggaggctaa taaaattgtc ttggaagcta atttgtggcc atcagccaag tcaacctgaa
     1981 aattaggata atggccgcta attttaagca cttcaccagc aactatctcg cctcctaatt
     2041 gtcgagcgtt attttctatt ttttgggcta attcccaacc gctaatactt acctcactag
     2101 ggaaattaca aattttgtaa gcttcagtca tcgccccgcc gggcaaaagg ccaataacct
     2161 gatgctctat tttatagcgg ctggcataaa tcgaagccgt taaaccagct ggcccagcgc
     2221 cgacaattat taaatcctta actcccatct ccatacttta agataatttg tctaaaatat
     2281 cttgacgcaa actcgcctgt tgacggactc caataaagct ggcaatctct tcaccatttt
     2341 tgaatagctt catgttgggt atgctttgaa tctgatactc cactgctaat ggttgattgt
     2401 caggatcatc aacattaact ttagcaattt ttattttatc acctaattct tgggccaaat
     2461 tctccaaaat gggagccatc atctgacaag ggccgcacca cggagcccaa aaatctacca
     2521 ataccggtat tttgctattt aaaacctcag tggcaaaatt agccggcgtt aattcta
//
LOCUS       DAOOIS010000039         2416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_34288_length_2416_cov_51.373571, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000039 DAOOIS010000000
VERSION     DAOOIS010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34288_length_2416_cov_51.373571"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1532
                     /gene="lysS"
                     /locus_tag="PK960_02785"
     CDS             69..1532
                     /gene="lysS"
                     /locus_tag="PK960_02785"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPD08119.1"
                     /translation="MAEKISEYETRRRRLELLQEAGIEPYPAQCQRKETNSQVLANFQ
                     AYEKSGEAVYLAGRLRSLREHGNLTFADLQDGTATIQLAFSKKELGDSYKNFLKFMDI
                     GDFVAVSGPVFRTHAGEPTVLVKEWQIISKALRPLPEKWHGLKDEDERWRKRYLDLLF
                     NDDLRVLFVKKAQFWQVTRQFMLDHGFLEVETPTLEITTGGAEANPFKTHYNDYDIDV
                     YLRISVGELWQKRLMAAAFDKTFEIGRVFRNEGSSPDHLQEFTNMEFYWAYANYLDGM
                     KLSRELYVNLAQQVFGTTKFNSKGYSFDLAGEWPELDYCGEIKRQTGIDVLEASDEMM
                     RHRLDELGVNYEGTSRERLIDTLWKYCRRNIAGPAFVVGHPKLVSPLAKSRSDNPELT
                     ERFQIIIAGTEMGNGYSELNNPLEQQARFQEQQKLLERGDTEAMMPDWDFIEMLEYGM
                     PPTCGFGFGERFFACLAGRSVREATLFPFVKPKVSSK"
     gene            1534..>2416
                     /locus_tag="PK960_02790"
     CDS             1534..>2416
                     /locus_tag="PK960_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPD08120.1"
                     /translation="MLDIKYIRENLAEVKENCRYRGAKVDLDRLISLDETRRSLQQEL
                     DDCRAKRNNWSKKAPTPQEIAAIKANNEAIKQGEVKLRQITEELEALLLTVPNMTDPA
                     VVRSLNPADNVILEVCGQPTQFSFLPQDHLALADKWGWLDFDRASKVAGAKFYYTAGD
                     LAWLELALISYALAVATRHGFKIMLTPDLAKTKIIEALGYNPRGESTQIYNLENSDLS
                     LIGTAEITLGGYHQDEVLPEKDLPLHYVALSQCFRTEAGSYGQFAKGIFRVHQFNKVE
                     MFIYSRPEDSAAEHQFLI"
BASE COUNT          700 a          397 c          598 g          721 t
ORIGIN      
        1 tttctttact tccccccata aattaggtac aatatagcca gcatattaag ctggttattt
       61 tgttttttat ggcagaaaaa ataagtgagt acgaaacacg aaggaggaga ttagaattat
      121 tgcaggaggc ggggattgag ccttatccgg cacagtgtca gcgtaaagag actaatagtc
      181 aagtgttagc taattttcag gcctatgaaa agtcaggcga agcggtttat ttagctggcc
      241 gtttgcggag cttgagagag catggcaatt tgacttttgc tgatttgcag gatggcacgg
      301 ctaccataca attggctttc agcaagaaag aactaggaga tagttacaaa aattttctta
      361 aatttatgga tattggtgat tttgttgcgg tgagcggtcc tgtttttcgt actcatgctg
      421 gcgagcccac ggtattagtt aaagaatggc aaattattag caaagcttta aggcccttac
      481 ctgagaaatg gcatggtctt aaagatgaag atgagcgttg gcgtaaacgt tatttggatt
      541 tgttatttaa tgatgatttg cgagttttat ttgtcaagaa agcgcaattt tggcaggtaa
      601 ctcgtcaatt tatgctggac catggatttt tagaagtaga aacgccaacc ttagaaatca
      661 ctactggcgg ggccgaggct aatcctttta agactcatta taatgattat gatattgatg
      721 tttatttgcg tatctcggtt ggagaactct ggcagaagag gttaatggcg gcggcttttg
      781 ataaaacctt tgaaattggc cgagtttttc gtaatgaagg cagtagccca gatcatttac
      841 aagaatttac taatatggag ttttattggg cttacgctaa ttacctggat ggaatgaaat
      901 taagtcggga attatatgtt aatttagctc aacaagtttt tggcactact aagtttaata
      961 gcaagggata tagttttgat ctagctggtg aatggcctga gttagactac tgcggggaga
     1021 ttaaacggca aaccggcata gatgttttag aagccagcga tgaaatgatg cgtcataggt
     1081 tagatgagtt aggggttaat tatgaaggga ctagccggga acgccttata gacaccctat
     1141 ggaagtattg ccgtcgtaat attgccgggc cagcttttgt ggtaggtcat cctaaactag
     1201 tgtcgcccct ggctaaatct cggtcagata atccggagtt aacagagcgc ttccagatta
     1261 ttattgccgg tactgagatg gggaatggct attctgaatt aaataatccc ttggaacagc
     1321 aagctcgttt ccaggaacag caaaaacttt tagaaagagg agataccgaa gccatgatgc
     1381 cggattggga ttttattgag atgctggagt atggtatgcc gcctacttgt ggttttggct
     1441 ttggagagcg gttttttgcc tgtttagcgg gacggtcagt cagggaagcg actttattcc
     1501 cctttgttaa acctaaagtg agctctaaat aatatgttag atattaaata catccgagaa
     1561 aatttggctg aagttaaaga aaattgtcgc tataggggag cgaaagtaga tttagatcgg
     1621 ctaattagcc tggatgaaac gcgacgtagc ctgcagcaag agttagatga ctgtcgggct
     1681 aagcgcaata attggtcaaa aaaagcgcca actcctcaag aaattgcagc cattaaggct
     1741 aataatgaag ctattaagca aggtgaagtg aaattgcggc aaattactga agaattagaa
     1801 gctttattac taactgtccc caatatgact gatccagcgg tcgtccggag tttgaatccg
     1861 gccgataatg ttattttaga agtctgtggt cagccgacac aatttagttt tttaccgcaa
     1921 gatcatttag ctttagcgga taaatggggc tggttagatt ttgaccgagc tagcaaggtg
     1981 gccggggcga agttttatta tacggccggt gatttagctt ggctggaatt agccttaatt
     2041 tcttatgctt tggcggtagc cactcgccat ggctttaaga taatgcttac tcctgattta
     2101 gccaaaacta aaattattga agcgctagga tataatccgc gcggtgaatc aactcagata
     2161 tataatttag agaacagcga tttaagctta attggaacag ctgaaattac ccttgggggg
     2221 tatcatcaag atgaggtatt gcctgaaaaa gatttaccct tacattatgt agctttatct
     2281 cagtgttttc gaactgaggc ggggagttat ggccagtttg ctaaaggtat cttcagagtg
     2341 catcaattta ataaagtaga gatgtttatt tactctcggc ctgaagatag tgcggcagaa
     2401 catcaattct tgatag
//
LOCUS       DAOOIS010000040         2398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_34670_length_2398_cov_37.251814, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000040 DAOOIS010000000
VERSION     DAOOIS010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34670_length_2398_cov_37.251814"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PK960_02795"
     CDS             complement(<1..808)
                     /locus_tag="PK960_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08121.1"
                     /translation="MSYNNTKNQKKTKVSSNLKLTTIILLLIVLLALGYSFSLWLSSS
                     KTKNLASEPTPVSSPKEKVSSTPLAASSPTSETRRKPNERFFSFSDLFSSTAWIDGGA
                     TTLVHDRSAMVFTFAPNYTWENLGSCVNLPAECEAVDNQQQVDGPLCRDRKHCLNLKA
                     GQLLYNGKPLSLPTEPLINLSIGLFGQRWLVGGVKVISEEEYQPLVWWFDGTKFEPLD
                     FKGYHDQPSHSSYQGYLAFGGWPDNVFIVYSARDGLAWQVRGQEIIDLSRY"
     gene            complement(876..1487)
                     /locus_tag="PK960_02800"
     CDS             complement(876..1487)
                     /locus_tag="PK960_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HPD08122.1"
                     /translation="MNKKTTWLIIGLVVVVIIIAIIASSGKQQTGTNKSSEANVPVAS
                     PEALSKEAIATPEEVQQAQPVIEGSSKVIDNVVVTPTGKPVKNDALAGTAEAPQRTDI
                     SNPDVLPPEAIKLTVSNQGFNPAQFEVKAGQVVTLGLFTTDGVHTIVFDDPSLQAIRL
                     NVNPEVGRAITFNAPPEAGEYSFYCDVPGHREDNGETGVMVVK"
     gene            complement(1517..2134)
                     /locus_tag="PK960_02805"
     CDS             complement(1517..2134)
                     /locus_tag="PK960_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HPD08123.1"
                     /translation="MNNKKTLWIIIGLAILALIIIVVATSTGTKQQSTTSAPEETNVQ
                     VAPTDLDTETPIATPEEVQQAQPVIEGSSKVIDNVVVTPTGKPVKNDAVPGSPEAPQE
                     TPPIAPEALPAGTIKLDVSAEGFNPVQFEVKAGQVVTLALSSVDNITHVLLFDDPVLA
                     GVAIGVGPNETRAITFNAPKAGEYGFHCDVPGHTARGESGKMIVK"
     gene            complement(2209..>2398)
                     /locus_tag="PK960_02810"
     CDS             complement(2209..>2398)
                     /locus_tag="PK960_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08124.1"
                     /translation="DVLMTAYGRPASLSYEIGDNQIIFYADHPTQFSYRLVGSRFDWR
                     QWPTLALDQSEPTSLIVP"
BASE COUNT          758 a          503 c          475 g          662 t
ORIGIN      
        1 aataacgact gaggtcaata atttcttggc ccctgacttg ccaagctaaa ccatctctgg
       61 cactatagac aataaaaaca ttatctggcc agccaccaaa agctaaataa ccctggtagg
      121 acgaatgcga gggctgatca tgataacctt taaaatcaag gggctcaaac ttagtgccat
      181 caaaccacca taccagtggt tgatattctt cctcactaat aacttttact ccaccaacca
      241 gccagcgttg gccaaataag ccaatgctta aattaattaa gggctcggta ggcaaagata
      301 atggcttgcc attatataat agttgtccgg ccttaagatt aagacagtgt ttgcggtctc
      361 ggcaaagtgg accgtcaact tgttgttgat tatctaccgc ctcacactcg gctggtaaat
      421 taacacagga acctaaattt tcccaagtat aattgggagc aaaagtaaaa accattgctg
      481 aacgatcatg gactaaagta gtagctcccc catcaatcca agctgtacta gaaaataaat
      541 cagaaaagct aaagaagcgt tcatttggtt tcctccttgt ctctgacgtg ggagaagaag
      601 cggcaagcgg cgtagaagag actttctcct taggcgaact aactggagta ggctctgagg
      661 ctaaattctt ggtcttagaa gaagacagcc aaagactaaa actatagcct aaagctagaa
      721 ggacaattaa tagtaaaatt atagtagtta gctttaaatt gctgctaact ttagtctttt
      781 tttgattctt agtattgtta tagctcacac tgtttcctaa gttaaaagca cagctaaaat
      841 aagctgtgcc tttaactaag atttacataa taattttatt taacaaccat cactccggtt
      901 tctccattat cttctctatg accagggaca tcacaataaa aagaatattc tccagcttca
      961 gggggggcat taaaggtaat agcccgtcct acttctggat taacattaag acgaatggcc
     1021 tgaagtgatg gatcatcaaa aacaatagta tgaaccccat cagtagtaaa tagtcccaaa
     1081 gtaacaacct gaccagcctt aacctcaaat tgagctggat taaatccttg gttgctaacc
     1141 gtcaatttaa tagcttcagg aggtaaaaca tcaggattag aaatatcagt acgttgtggc
     1201 gcctcagctg tgccagctaa agcatcgttt tttactggct taccagtagg tgtaaccacc
     1261 acgttgtcaa tgaccttact actaccctct attactggtt gggcttgctg tacttcttct
     1321 ggagtagcaa tagcttcttt gcttaaagcc tcaggcgacg ctactggaac attagcctct
     1381 gaggatttat tagtaccagt ttgttgctta ccgcttgacg ctataatagc aataataatt
     1441 actacaacca ctaagccaat aataagccag gttgtctttt tattcatatt tacaagatca
     1501 aaattttgtt aacctattat ttaacaatca tcttgccaga ttcaccccgg gcggtatgcc
     1561 ctggcacgtc acaatggaaa ccatattccc cagccttagg agcattaaaa gtaatagctc
     1621 gagtctcatt aggaccaact ccaatagcta caccagctaa gacagggtca tcaaaaagca
     1681 aaacatgagt aatattgtct actgaagaca aggccaaagt aacaacctga ccagccttaa
     1741 cctcaaattg aaccgggtta aagccctcag cgctaacatc taatttaata gtaccagccg
     1801 gtaaggcttc gggggcaatg ggtggtgttt cttgtggtgc ctcaggtgaa ccaggcactg
     1861 catcattttt tactggctta ccagtaggtg taaccaccac gttgtcaatg accttactac
     1921 taccctctat tactggttgg gcttgctgta cttcttctgg agtagcaatg ggggtctccg
     1981 tatccaggtc agtaggagcg acctggacat tagtctcttc tggagcagaa gtggtggatt
     2041 gttgcttagt accagtagaa gtggctacca caataataat gagagccaag atagctaagc
     2101 caatgataat ccatagggtt tttttgttgt tcataggttc ttagttaata aaaaattagt
     2161 taattaaaga atatgattat tatagcacat taaaatgaaa aactaaaatt aaggcacaat
     2221 taaagaggtt ggctcagatt gatctagggc aagagttggc cattggcgcc aatcaaaacg
     2281 agaaccaact aaacgatagg aaaattgggt cggatgatca gcataaaaaa ttatttggtt
     2341 atctcctatt tcataagata gtgaggccgg acggccatag gcggtcataa gcacatca
//
LOCUS       DAOOIS010000041         2389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_34858_length_2389_cov_54.381320, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000041 DAOOIS010000000
VERSION     DAOOIS010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34858_length_2389_cov_54.381320"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..788)
                     /locus_tag="PK960_02815"
     CDS             complement(18..788)
                     /locus_tag="PK960_02815"
                     /inference="COORDINATES: protein motif:HMM:NF021390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HPD08125.1"
                     /translation="MTSNKKTIIWLIIAILLIVIVLLIFSRPVSAPLVSTTSSSTEIV
                     SSSAPEETGNSTALIHATFICPGGEVINAVFHNEENSFVDLALPDGRLLRVPQALSAD
                     GARYATENEMFVFWNKGNEAFIEENGTTTLKDCIASEEPITTTTPDVATVANPASLYC
                     QEKGGQLEIASRPDNSQYGLCYFDDNRACEEWAMFRGDCPIGGVKTTGYDTEAQKFCA
                     WSGGHTLAVPNAVCTFEDGSSCLADDFYTGACQKGNNN"
     gene            complement(872..1351)
                     /locus_tag="PK960_02820"
     CDS             complement(872..1351)
                     /locus_tag="PK960_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08126.1"
                     /translation="MNNDNVNFTRQLVKGKIAEAIFAQMFRQSGNFTVLEFGYEKVIP
                     EIIQQGYSENNPMIETLRTAPDFAVIDHTTREVKLVEVKYQRRLNSDYTLRAAIRMSQ
                     CWNPSYLFVATLDGFYMDKISNIIKNDGAITPLKGPDMDLDLQNSYLQILKDFEQAN"
     gene            complement(1370..>2389)
                     /locus_tag="PK960_02825"
     CDS             complement(1370..>2389)
                     /locus_tag="PK960_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08127.1"
                     /translation="GGKEDQHNDILKREDLSKRGGGKGMFSEQVQGGSIYTGKKDGKV
                     HGGKEDQHNDILKREDLSKRGGGKGESMFSEQVRENFIYTGEKINKQAIQDREKMVGK
                     VDRRLKYETRPDKASMREVIRNIRKIILDISEIKTQNVDLKGTVRNFAKRDFRFDYNR
                     EKEKQPEIVLLIDVGGPVDEWSPLVKEVTEAMVEGLTKLEVYLFHNNLYGYVWKPDPQ
                     NLLASTYAKPDSLIDIKSIIKRRKNVIIYGDAEMSYSELNRDCWPPHDNEEGIKKFGM
                     GGEECLNFIKKKANSVVWLNPIFKKEWQNHDFSGTIQTISNIISMHDLSVGGVEDAIK
                     DLMKQ"
BASE COUNT          727 a          513 c          351 g          798 t
ORIGIN      
        1 tttaactaat attagcccta attattgttt cctttttggc aggcaccggt ataaaaatca
       61 tcagctagac aggaagagcc atcctcaaaa gtacaaacag cattaggaac ggcaagagta
      121 tggccaccag accaagcaca aaatttttga gcttcagtat cataaccggt agttttaact
      181 ccaccaatag gacaatcgcc acggaacatc gcccattcct cgcaggctcg attatcgtca
      241 aaataacata gtccgtattg gctattatcc ggccgtgaag ctatctccaa ttgccctcct
      301 ttttcttggc aataaagact ggctggatta gccacagtag ctacatcagg agttgtagtg
      361 gttattggct cctcactagc gatacaatct tttagggtgg ttgtaccatt ttcttcaata
      421 aaggcctcat tacctttatt ccaaaagaca aacatttcat tttcggtagc ataacgcgct
      481 ccatcagcag ataaagcttg gggcacacga agtaaacgtc catcaggtag ggctaaatca
      541 acaaaactat tttcttcatt atgaaaaact gcattgatta cctcaccacc tggacaaatg
      601 aaggtagcat gaattagagc tgtactattg cctgtctctt caggagctga tgaactaact
      661 atctcggtag aagatgaagt ggtagaaacc agtggagcgc taactggacg actaaaaatt
      721 aataaaacaa taacaattag caatatagcg ataattagcc aaatgatagt ttttttgtta
      781 gaagtcataa aattattaaa tcaaaattta tcaaaagcct ttggtgagct aactttcctt
      841 actaggtttt tatattaatt tgacaaatta actagttagc ttgctcaaaa tcttttaata
      901 tctggagata actattttgt aaatctaaat ccatatctgg gccctttaaa ggagtaattg
      961 caccatcatt tttaatgata ttgctaatct tgtccatata aaaaccatct agggtagcaa
     1021 caaacaaata agaaggattc caacactgac tcattctaat tgctgctctt aaagtataat
     1081 ctgagtttaa tcgccgctga tattttactt ccactaattt tacctcccga gtagtatgat
     1141 caatgacagc aaaatcgggt gcggtcctaa gagtttcgat catcggatta ttttcactat
     1201 atccttgctg gattatctca ggaatcactt tctcataacc aaactctaag acagtaaaat
     1261 tgccagattg tctaaacatt tgagcaaata tggcctcagc tatcttacct ttaacaagtt
     1321 gcctagtaaa atttacattg tcattattca taatttaata agaagagttc tattgtttca
     1381 ttaaatcttt tatagcatcc tcaacaccgc caacgctcag atcgtgcatt gaaataatat
     1441 tactaatagt ttgaattgtt ccagagaaat catggttctg ccactctttt ttaaaaatag
     1501 gatttagcca aaccacagaa ttagcctttt tctttataaa atttaagcat tcttctccac
     1561 ccatgccaaa ttttttaatc ccttcttcat tatcatgcgg aggccagcaa tctctattta
     1621 attcagaata ggacatctct gcatcaccat atataataac attctttctt cgttttataa
     1681 ttgattttat gtcaattaat gaatctggct tagcataggt agaagcaagt aaattttgcg
     1741 ggtcaggttt ccaaacatat ccataaagat tattatgaaa aagataaacc tctaatttgg
     1801 tcaaaccctc aaccatcgcc tcggtaactt cttttactaa cgggctccat tcgtctaccg
     1861 gtcctccgac atcaattaat aaaactattt cgggctgctt ctctttttct ctattatagt
     1921 caaaccgaaa atctctcttt gcgaaatttc tcactgtacc ttttaggtca acattctgag
     1981 ttttgatttc agaaatatca agaattatct tgcgaatatt tctaattact tcacgcatac
     2041 ttgctttatc gggtcttgtc tcatatttta atcttctgtc aacttttcca accatttttt
     2101 ctcgatcttg aattgcctgt ttattaattt tttcgcctgt ataaataaag ttctctcgaa
     2161 cttgctcgct aaacatagac tcgcctttac cacctccccg ttttgacaaa tcttctctct
     2221 ttaaaatatc gttatgctga tcttctttgc caccgtgaac tttaccatcc tttttacctg
     2281 tataaataga gcccccttga acttgctcac taaacatacc tttaccacct ccccgttttg
     2341 acaaatcttc tctctttaaa atatcgttat gctgatcttc tttgccacc
//
LOCUS       DAOOIS010000042         2234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_38290_length_2234_cov_65.987150, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000042 DAOOIS010000000
VERSION     DAOOIS010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38290_length_2234_cov_65.987150"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PK960_02830"
     CDS             <1..609
                     /locus_tag="PK960_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPD08128.1"
                     /translation="KGFDLRGKTIGVVGAGKIGLHLIKMARGFDMNVLAYDVNRNDFL
                     ADVLGFKYVSLEELLKHSDIISLHVPENKFTHHLINRQNIYLIKKGALLINTARGGIV
                     ETEALIEALDKKILAGAGLDVIEGENLIMEEKQLAYDKETAKEMMSLVKNHILLSKDN
                     VIFTPHIAFYSEEAVERIMEVSVENVKAFIQGQPQNVVRSLN"
     gene            609..1859
                     /locus_tag="PK960_02835"
     CDS             609..1859
                     /locus_tag="PK960_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HPD08129.1"
                     /translation="MEQILDLHLHSRFSRACSANLTLPNIEQACIRKGIDIVGTADFT
                     HPQWFKEIGEQLVEIDHSGLYHLAERPDSPIKFLLSTEVALIYKQGDKVRRLHIVIHA
                     PNLEAVRLFNQTLLERGCNLKSDGRPILGLTAPELVKICRSINPYFIIYPAHIWTPWF
                     SVFGSKSGFDRLEDCFREETEFIYAYETGLSSDPPMNWRWSALDNLVLLSNSDAHSLS
                     NLAREANVIDLPELTYTAFCNILKNRDKKKIIATLEFFPEEGMYHFDGHRQCGFGCSP
                     EETKKLHGLCPKCRKPLTIGVLNRVEELADRPVGYRPAEAPDFEKLVELDKIIAESLG
                     IASRNSKKVQEVYGEMIATLGSELTILRKVKVEEIAQHFGSRLAQAIERVRRGQLVVN
                     PGFDGQYGQIKIFSPQEKQPLSLF"
     gene            1864..>2234
                     /locus_tag="PK960_02840"
     CDS             1864..>2234
                     /locus_tag="PK960_02840"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPD08130.1"
                     /translation="MKKAFTLVELLVVIAIIGILSTLSVVALNSARAKARDARRLSDI
                     RQIQMALEMYYDSVGIYPVSLTPGSPLSYNGLTFLAKVPDDPLSSQHYIYAQTENGQG
                     YTIDFTLETKSADYEPGNYQAT"
BASE COUNT          664 a          365 c          496 g          709 t
ORIGIN      
        1 aagggttttg atttacgagg taaaactatt ggggtagttg gtgccgggaa aattggccta
       61 cacttaatta agatggcgcg gggttttgac atgaatgtcc tagcttatga tgtgaaccgc
      121 aatgattttt tagctgacgt cttaggcttt aaatatgttt ctttagaaga attattaaag
      181 cattccgata tcattagttt acatgtgcca gaaaataaat ttacccatca tttaattaat
      241 cggcaaaata tttatttaat taaaaaaggc gctttactaa ttaatacggc gcgaggcggt
      301 attgttgaaa ctgaggcttt aattgaggct ttagacaaaa agattctagc tggtgccgga
      361 ctagatgtca ttgaaggaga aaatttaatt atggaagaaa agcaactggc ttatgataaa
      421 gaaacggcta aagaaatgat gtcattagtc aagaatcata ttcttttaag caaagacaac
      481 gttattttta cacctcatat tgctttttat agtgaagagg cggtggagag gattatggaa
      541 gttagcgtgg agaatgttaa agcttttata caaggtcagc cacaaaacgt tgttagaagt
      601 ttaaattaat ggaacaaatt ttagacctac atttacattc tcgttttagt cgcgcttgtt
      661 cagctaattt aactttacct aatattgagc aggcttgtat ccgcaaaggg atagatattg
      721 tggggacggc tgattttact catccgcagt ggtttaagga aataggagaa caattggtag
      781 aaattgatca ttctggttta taccatttag ctgagaggcc agatagtcct attaaatttc
      841 tgttgagtac tgaagtggct ttaatttata aacaaggaga taaggtaagg cgcctgcaca
      901 ttgtaattca tgcccccaat cttgaagctg tccgtctttt taatcaaacc ttgctagagc
      961 gaggttgtaa tttaaaaagt gatggtcggc caatattggg cctgaccgca ccggaattag
     1021 taaaaatttg ccgaagcatt aacccttatt ttattattta tccagcccat atctggacgc
     1081 catggttttc agtttttggg tctaaatctg gttttgaccg tctggaagat tgttttagag
     1141 aggaaactga gtttatttat gcttatgaaa ctggtctttc gagcgatccg cccatgaatt
     1201 ggcgctggtc agctttagat aacctagttc ttttatctaa ttctgacgct cattctctta
     1261 gtaatctagc acgggaagcc aatgttatag atttgccaga attgacctat actgcttttt
     1321 gtaatattct taaaaataga gataagaaga aaattattgc tactttagaa ttttttcccg
     1381 aagaggggat gtatcatttt gatggccatc gtcaatgtgg ttttggttgt tcacctgagg
     1441 aaaccaagaa actgcacgga ctttgcccta agtgtcgtaa acctttgact attggggtgc
     1501 tgaatcgagt ggaagagtta gcagatcggc cggtgggcta tcggccagcc gaggctcctg
     1561 attttgaaaa attggtagaa ttagacaaaa ttatagctga aagtttgggg atagctagcc
     1621 gtaattctaa aaaagttcag gaagtatatg gggagatgat tgctactctg ggcagtgaat
     1681 tgacaatctt aagaaaagtt aaagtggaag agattgcgca gcattttggc agtcgcttag
     1741 cgcaagccat tgagcgagta cgccggggac aattagtagt aaatcctggc tttgatggcc
     1801 aatatggtca aataaaaatt tttagtcctc aggaaaagca gcccttaagt ttgttttaag
     1861 cctatgaaaa aagcttttac cttagtggaa ttattggtgg ttattgccat tattggtatt
     1921 ctttctactt tatcagtagt ggctctaaat agtgccagag ctaaagcccg tgatgctcgt
     1981 cgcctttcgg atattagaca aattcaaatg gccctagaga tgtattacga tagtgtcggg
     2041 atttatcctg tatctttaac tcctggcagt cctttatcat ataatggttt gacttttctg
     2101 gctaaggtgc cggatgaccc cttgtctagt cagcattata tttatgccca aactgaaaat
     2161 ggacaaggtt atactataga ctttacccta gaaactaaat cagcagatta tgagcccggc
     2221 aattaccaag caac
//
LOCUS       DAOOIS010000043         2221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_38649_length_2221_cov_36.971837, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000043 DAOOIS010000000
VERSION     DAOOIS010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38649_length_2221_cov_36.971837"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            536..1048
                     /locus_tag="PK960_02845"
     CDS             536..1048
                     /locus_tag="PK960_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HPD08131.1"
                     /translation="MANVLIIHGHENEGWTLSFDAEARCRKAIELIRSGYPFDRVICT
                     GGLFSKKQQGITNAAAMAHRIRQEITNIEIEEENESLTTIDNCEKVIQMVGKDDDFDF
                     VVISSDYHAVRTIIIWYLAGKKIKFVAVPGPKNVQRCIVETVGIAVAICWHFGFKWPE
                     MVFRKMFRQV"
     gene            1947..>2221
                     /locus_tag="PK960_02850"
     CDS             1947..>2221
                     /locus_tag="PK960_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08132.1"
                     /translation="MGKAGLELKKEILLALGKTPMIKDGKLYIEPNAWFVPIKNNYPA
                     LEAEYQGLEPTETPINKTQQRLQPLFVHAGSRGGIRTHDQLVNSQLR"
BASE COUNT          739 a          426 c          410 g          646 t
ORIGIN      
        1 cttaacaaaa ataaccatat tctgctagga ttattttgac gggtcttgga gcatagttgg
       61 attttcttag aagaaaaaac cgctgggcga accatatagt aatgaatagt ttcttccatt
      121 tttaatcggc aggtcttcca gcagtttcaa ggtagacatc attctcttta aacgaccggc
      181 aacctcaacc ggtgagccga cggttaatct ttctatagcg acaaaattat ttacattgtt
      241 attaaaaact acccacaaat tcttaaaaaa gtttcatcat tcctaaaaaa gaaaacccat
      301 ggagtacgcc ttcctctttc cctttcgagc tagattagcg ccttccgaac ggattgcttc
      361 gtagttgtcg gcagatcatt gggccagatc cctaactgcg ctctttataa atatttaact
      421 gtatttttat tacaatagaa aaactgaatc ttacttagta actattgaca aatactatca
      481 taagtgatat tatctattat caacgtacat aaaaaatagt acacaggagg tcatcatggc
      541 caacgtttta attattcatg ggcacgaaaa tgagggatgg accctcagtt ttgatgccga
      601 agctcgatgc cgaaaggcca tcgaattaat tcgttcaggc tacccgtttg atcgggtgat
      661 ctgcacgggc gggttatttt ccaaaaagca acagggaata accaatgcgg cggctatggc
      721 tcaccgcatc cgccaggaaa taacaaatat tgaaattgaa gaagaaaatg agagtctgac
      781 caccattgat aactgcgaaa aagttattca aatggttgga aaagatgacg actttgactt
      841 tgttgttatc tcgtcagact atcatgccgt gcgaacaatc attatatggt atttggctgg
      901 caaaaaaatc aaatttgtgg cagtacccgg cccaaaaaat gtccaaagat gcatagtgga
      961 gacggtcggt atagccgtcg ccatctgctg gcactttggc tttaagtggc cggagatggt
     1021 gtttaggaaa atgtttcgtc aagtctagcc cccccctttt taactaaacc ataagcccga
     1081 tgctagtcat tccgggcttt tttattttgt catattattg acgatttaag ccaacaagtc
     1141 ttaaacaaac tataaaatga gtatacttta tgtcttttaa aattattaat agtcatcata
     1201 tccagacaat tccatattag acaatatact tcctgatata aaaagagttt tttacatctt
     1261 ctattttata gtattcatta ttaagataga tacatcaaaa aagctctttc ttctaaggcg
     1321 atcgaggaat aatccctatt ttctatcccc tcgttattaa gaatattcgg ttttaatttt
     1381 tgaaagaaaa aattgcccat cgcaattaat atctttagtt taatatgggg taacgaaaat
     1441 ctgactatag tactgaaaga caattccagt ctcgctaaaa ctgttttaaa acttacagaa
     1501 tccggaagga aatatctttg tagccaattc aagtctgtta taattcttgt cttttactac
     1561 aagcccccgc ggataccgta aagctaacca aagagccgga ttgctttccc tagaataaca
     1621 gtatttcctt aaaagaaaag actatatgat tgttttttag tatgtgttat gctcggtaaa
     1681 tataaggtgg ataatattca caagtatcca aagtttaatg aatggggggc ataaataggt
     1741 ctcattaact aaatattggc aaagaatagt gaacaggtaa acaaaaagat ggctataaaa
     1801 ggctatcaag actgccaaat acagattaga ttggattatt gatacaccag ctcaccgaaa
     1861 caagagctaa aaaatggctt gagctgactg agaaaacttt tacttttgct acctatgcac
     1921 gaactgcttt tataagggcc aataatatgg gcaaagcagg gttagaactg aaaaaagaaa
     1981 ttttactggc tctcggaaaa acgccgatga ttaaagacgg aaagctctat attgaaccaa
     2041 atgcatggtt cgttccaatc aaaaataact accctgcatt agaggctgaa tatcaagggt
     2101 tagaaccaac agaaacgcca ataaataaaa cacaacagag gcttcaacct ctgttcgtac
     2161 acgctggctc ccggggtggg attcgaaccc acgaccaatt ggttaacagc caactgcgct
     2221 a
//
LOCUS       DAOOIS010000044         2204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_39072_length_2204_cov_54.863192, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000044 DAOOIS010000000
VERSION     DAOOIS010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39072_length_2204_cov_54.863192"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="PK960_02855"
     CDS             <1..1275
                     /locus_tag="PK960_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08133.1"
                     /translation="AATIVVPLSYGRVKPYYDGDAINYRGRLFICSTNMNRLQLLEAN
                     DGHIILLAELTPDKSGYLSGDDFYNCVFHEEGNELFLYAVNGRYLYKYNVNNPPKPEL
                     VSKVKDNSWDWFRSVAVFNGRITTAGTKGVKIWNNDLQVIDNLTVKLDNPFNYTLSRD
                     FLVAVDGAELKMYSLQSREWVNSVSLTVKENSNRQIYNDSISSSIYVVDDWAVKQFDF
                     NGNLLNTFKFTGTNGYDIVPSTDGQKLYFSDGIGVVRLNKANLQPEIWSYVRDMGVSD
                     GWSMGLKLVSWQGRDHLVVFNNGAITVLNDQLKKVASYNSTEERPIEQVTYSEPLSLS
                     LDKNRAPVGSEVSLRGTGFLPNESLEIKFLSNPQATSTVVTNNLGRFQKILTVPQIAS
                     GVNPSINPWPTDIRVTGKTSLKTYSINFQIEY"
     gene            1297..>2204
                     /locus_tag="PK960_02860"
     CDS             1297..>2204
                     /locus_tag="PK960_02860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPD08134.1"
                     /translation="MRIYFIGQKGIPAKSGGVEKHVEALATNLAQNGQEVFVYTRPHY
                     TNKKLTEYQGVKLISRPSCPTKHLDTFTHTLISTIDVLFRRADIVHFQAIGPASLIWI
                     VKLFKPKTKVIFTFHCQDYYHQKWGRLARFYLRLGEVVGCRLADEVIVVSHNLADYVA
                     QKYGREAKYIPNGVMITDKSVHSNILSQLGLEPQGYILAVTRLVKHKGLHYLIEAFHK
                     LDSDSKLVIVGDGAHTDDYVNHLIELAQADPRIIFAGERSGNDLAQLFRGARLFVQPS
                     ESEGLSIALLEAMAYGVPVLVSDIPG"
BASE COUNT          645 a          359 c          495 g          705 t
ORIGIN      
        1 gccgctacta tagttgtacc cctttcttat gggcgggtta aaccatatta tgatggtgat
       61 gctattaatt atcgcgggcg tttatttata tgttcgacta atatgaaccg tctgcagcta
      121 ctggaagcga atgatggaca tattatttta ctggctgagc taactccgga taaatctggt
      181 tatctgtcgg gtgatgattt ctataactgt gtctttcacg aagaaggtaa cgagttattc
      241 ctttatgccg ttaatggtcg ttatctttat aaatataatg ttaataatcc cccaaagcca
      301 gaactagtta gtaaggttaa ggataattcc tgggattggt ttagatcagt agcagtcttt
      361 aatggacgaa ttactactgc cggtactaag ggggtaaaaa tttggaataa tgatctgcaa
      421 gttatagata atttaacggt caaattagat aatcctttca actatacctt aagtcgcgat
      481 tttttagtgg cagtggatgg ggcagagctt aaaatgtata gcctacaatc aagagagtgg
      541 gttaatagtg ttagtttaac agttaaagag aatagtaatc gtcaaatata taatgactcg
      601 attagcagct caatttatgt cgttgatgat tgggcggtta aacagtttga ttttaatggt
      661 aatttgctta atacttttaa gtttaccggt actaatggtt atgatattgt gccctcaact
      721 gatggccaaa agctttattt tagcgatggt attggggtgg tgcggctaaa taaggccaat
      781 ctgcaaccag aaatttggtc ttatgtaaga gatatggggg taagtgatgg ctggtcaatg
      841 ggtcttaaac tagttagttg gcagggccgt gaccatctag tagtttttaa taatggagct
      901 atcacggtgc ttaatgacca actaaagaaa gtagctagtt ataattctac ggaggaaagg
      961 cccattgaac aagtgacata tagcgagccg cttagcctta gcttagataa aaatcgggct
     1021 cctgtaggca gtgaagtttc gctgcgcggt actggttttt tacctaatga atccttagag
     1081 attaaatttt taagtaatcc tcaggcaaca tcaacagtag ttactaataa tttaggacgt
     1141 tttcaaaaaa tcctgactgt tcctcagata gccagtgggg ttaatccttc cattaatccg
     1201 tggccaactg acattagggt aactggtaaa actagcctga agacttatag cattaatttc
     1261 caaatagagt attaattttg agaccaatgc ttaattatga ggatttattt tataggacaa
     1321 aagggcatac cagctaaaag cggtggagta gaaaaacatg tggaggcttt agctactaac
     1381 ctcgcccaaa atggccagga ggtttttgtt tatactaggc cacattatac taataagaag
     1441 ttgactgaat atcagggagt aaaattaatt agccggccta gttgtcctac taagcattta
     1501 gatactttta cccatacttt aattagtacc attgatgtcc tatttcggcg agccgatata
     1561 gtacattttc aagctatcgg gccagcctca ctaatatgga tagtgaaact ctttaaacca
     1621 aaaactaaag tcatttttac ttttcattgc caggattact accatcagaa gtggggacgg
     1681 cttgctcgtt tctacctccg attaggagaa gtggttggtt gtcgtctggc tgatgaagtt
     1741 attgttgttt ctcataacct ggcagattat gtggctcaga aatatgggcg tgaggctaaa
     1801 tatataccca atggggtaat gattactgac aagagtgttc attcaaatat tttatcccaa
     1861 ctaggattag agccgcaagg ctatatttta gcggttactc gtttagtaaa acataaaggg
     1921 ttacattatt taattgaagc ttttcacaag ctagattcag atagtaagct ggtgattgtt
     1981 ggggatgggg ctcatactga tgattatgta aatcacttga tagaattagc tcaggctgat
     2041 ccgcggatta tttttgctgg cgaacgttca ggtaatgatt tggctcagct gtttagggga
     2101 gctaggttat ttgttcaacc atcagagtca gaggggctat ctattgcttt acttgaagcc
     2161 atggcttatg gtgtgccagt tttagtaagc gacattcctg gcaa
//
LOCUS       DAOOIS010000045         2176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_39798_length_2176_cov_51.879302, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000045 DAOOIS010000000
VERSION     DAOOIS010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39798_length_2176_cov_51.879302"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PK960_02865"
     CDS             complement(<1..923)
                     /locus_tag="PK960_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08135.1"
                     /translation="MALPTGLLLIITILSFAVLAYGADMLVANIKMIASHYHLSIFLL
                     GIILGFFTSIPELILGLTAVHRGVASLSLGNLLGGIIVLFGLILGLAIIVHRTIKTDG
                     QSSTIAPSILILLLPLMLGLKGSLNHWDGLIIIIAYIAVVLYSARTESSAKQAQVSKP
                     PRLASRHFLFSLLGLGLIIGTSQVIVSTMTELMERWQWPPLVAGVLLLAIGTNLPEIV
                     VTIESLIKRASDLSISNLIGSAIGNVVVAGILLQFGSFSIQPFMAFVVTASTICLLSG
                     FLLLFYKTNKQFSRWEGIILLCIYLLFLVAEL"
     gene            complement(929..1426)
                     /locus_tag="PK960_02870"
     CDS             complement(929..1426)
                     /locus_tag="PK960_02870"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HPD08136.1"
                     /translation="MRLPVRKGGKYTFIKPDPYLTSAKAAELKKRLEWLKKVAQPRAI
                     KEVSRLAEMGDFSENAAYQMAKGRLRGINQEIIELHDYLNQVVIIEPPRDNRVGLGSR
                     VEVEMDGENKSFLILGSKETNPSKGIISHNSPLGAALLGRQAGEEVNLKLANRWLKIR
                     IIKVN"
     gene            complement(1587..>2176)
                     /locus_tag="PK960_02875"
     CDS             complement(1587..>2176)
                     /locus_tag="PK960_02875"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08137.1"
                     /translation="LLDFQKLDSYSKEIISRLSLNELLEQSLAWARKYDPPLAQAMSR
                     DLAYTTEILNIDHAGERRRKDIAKWSELRAVIGYFYDDIWAATPLDLASQLGDYSLSE
                     IRTIAQAVAASYKPQDSQAEWLAKLRQLSQTLGYAPDTISYRQNPQRYKGSFGDVAKI
                     IRVLLVGRNQSPDLYAVMQVMGQERVRTRLANFTG"
BASE COUNT          735 a          492 c          396 g          553 t
ORIGIN      
        1 agctcggcta ctaaaaatag tagatagata caaagcaata taatcccctc ccaacggcta
       61 aactgcttat ttgttttata aaacaaaagc aaaaagccac taagcaggca gatggtactg
      121 gcggtaacaa caaaagccat aaatggctgg atggaaaatg acccaaattg taataatata
      181 ccggcaacaa ctacattgcc gatcgccgaa ccaattaagt tactaatgga taaatcactc
      241 gcccgtttaa ttaaactctc tatagtaaca acaatctccg gtaaattagt gcctatcgct
      301 agcagcaata ctccagccac cagtggcggc cactgccaac gttccattag ctcagtcata
      361 gtgctgacaa tcacttgact agtgccaata attaggccaa gacccaataa agaaaataga
      421 aaatggcgac tggcaagacg aggtggctta gagacctgcg cctgtttcgc cgacgattca
      481 gtcctagcgc tatataacac tacagcaata taggctataa ttataataag gccgtcccag
      541 tgattaagtg agcccttgag acctaacatt aaaggcaaaa gtagaatcaa gatgctcggg
      601 gcaatagtag aagactggcc gtcagtttta attgtgcgat ggactataat ggctagtccc
      661 aaaattaaac caaataagac aataatacca cccagcaaat tacccaaaga tagactagct
      721 accccgcgat gtactgctgt taaacctaaa ataagctctg ggatactagt aaaaaagccc
      781 aagataatac ccagtaaaaa aatgcttaaa tgataatgac tggcaatcat cttaatattg
      841 gctaccagca tatcggcgcc ataagctaaa acagcaaacg ataatatagt aataataagg
      901 agaagaccag tggggagagc cataaatatt aattaacttt aataatgcga attttaagcc
      961 agcgattagc tagcttaagg ttgacctcct cgcccgcttg ccggcccagg agagctgccc
     1021 ctaagggtga attatgagaa ataatcccct tactagggtt agtttctttc gaacctaaaa
     1081 ttaaaaaact tttattttct ccatccattt ctacctctac tcggctacca agtcccaccc
     1141 gattatcccg aggtggttca ataataacta cctgatttaa ataatcatga agctcaataa
     1201 tctcctgatt aatccccctt agcctacctt tagccatttg gtaagccgca ttttcagaaa
     1261 aatctcccat ctcggccaga cggctaactt ctttaatggc tcgaggctgg gccactttct
     1321 ttaaccattc caagcgtttc tttaattcag ctgccttagc tgaagttaaa taaggatcag
     1381 gcttaataaa agtatactta ccgcctttac gaactggtaa acgcatataa aataataact
     1441 actaaaataa tagcaaaaat aaccaaaaaa tcaaaataaa agaaaaaccc caaggtcgaa
     1501 ggagttgtga ccttggggac ttgattagga gggtactgat gaatgataca aataagtata
     1561 taaaataata aaaaatttgt caactgttat ccagtaaaat tagctaaccg ggttcgcacc
     1621 cgttcctggc ccattacctg catcactgca taaaggtcag gcgactgatt acggcccacc
     1681 aaaaggaccc gaataatttt agccacatcg ccaaaactcc ccttatagcg ttgcggattc
     1741 tgacgatagc ttatagtatc tggcgcatag cctaaagttt gacttaactg ccgcaattta
     1801 gccagccact cggcctgact atcttgaggc ttataactag cagctactgc ttgagcaatg
     1861 gttctaatct ccgacagcga ataatcccct aattggctag ctaaatctaa gggtgtagcc
     1921 gcccagatat catcatagaa ataaccaatg acggcacgaa gttcagacca tttggcaatg
     1981 tctttccgcc gccgttcccc ggcatggtca atatttaaaa tctcagtagt ataagctaaa
     2041 tcacgactca tcgcttgggc caagggtgga tcatattttc tagcccaagc tagcgattgt
     2101 tctaaaagtt cattgagaga cagacggcta ataatttctt tactataact atctaatttt
     2161 tgaaaatcta acaaag
//
LOCUS       DAOOIS010000046         2171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_39909_length_2171_cov_55.030718, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000046 DAOOIS010000000
VERSION     DAOOIS010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39909_length_2171_cov_55.030718"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2171)
                     /locus_tag="PK960_02880"
     CDS             complement(<1..>2171)
                     /locus_tag="PK960_02880"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPD08138.1"
                     /translation="WYRFILKVGVYASLVSLFLVNKHFYFPYITSKQLYFNILIEILT
                     VVWLAYIIKYPSERPKKSWLTIGWAVYLLIVLITCFTGVDFNLSFWGDLERMLGWFPL
                     FHFFLLYLIVITVFRNWRDWQPLLMAMVGAGTMLSLIGLFQNYNASTLGNAAYMGTLM
                     LMTMFITALLISRSRNWLVSLPYWLVLILLFVGFVRADISGAYAGLAVGILVYAGLMT
                     FLNHKKKVRQWGLVVVGVLVIGVGLLFAFRWSPALDNTQLGKIIRDFSLENPTLNTRF
                     YSWKWAWRDLGSHPLLGVGYGNYALVFDRQFEGDFYRFGKGEDYFDRAHNNVVEIATT
                     TGLLGLAAYLFMFTWAVIYLIKAYREDKIKLPEFAALAAFLASYFVQNLALFDCLASY
                     IFLMIILGLINNLYHRPEGDKKEKDQGWTSKEIWVLVGGGLLMAWLIWAANITTIKML
                     NGTIDGIIEFSQGKMMSGLETFKKAHAEGSPLVRDSRSAMINSLLSTSYYLDNLTDVQ
                     RTAIFSYAIDQGKKNLEYNPKDNFLNMQLAQLLELASQASRNNLSVSYAREGLQYVEK
                     SIENGREHILPYYVKAQLLMDLNQVDEAIATLKEALAISTDYWDTYCHLSRIELQAQV
                     NVTEAYDFLDKCLDKGGATLLGPGNYLNEAIKHYDQAGDIDRLTMLIEQLARIYPQGA
                     EIWLRLADLYHKQGRDEESQYAAQQAVSLKPEYKAQVEEILG"
BASE COUNT          726 a          529 c          377 g          539 t
ORIGIN      
        1 cccaatatct cttccacctg cgccttatat tccggcttta agctcactgc ctgctgagcc
       61 gcatactgag actcttcatc tctcccctgc ttatgatata aatcagctag tcttaaccaa
      121 atctctgccc cctggggata aattcgggct agttgctcaa tgagcatggt caggcggtca
      181 atatccccgg cctgatcata gtgtttaatg gcttcattga gataattacc tggccccagt
      241 aaggtcgctc cccccttgtc taaacattta tctagaaaat cataggcctc agtcacatta
      301 acttgagcct gcagttctat ccggcttaaa tgacaataag tatcccagta gtcagtgcta
      361 atggctaaag cttcttttaa agtggcaatc gcttcatcga cttgatttaa atccataaga
      421 agttgtgcct tgacataata gggtaaaata tgttcccttc cattttcaat agacttttcc
      481 acatattgta atccctcacg ggcataacta accgacaaat tattacgact agcctggcta
      541 gctaattcta acagctgggc taattgcatg tttaagaaat tatctttagg attatactct
      601 aaattcttct tgccttggtc aatggcataa ctaaaaatag cggtccgctg aacatcggtt
      661 aaattatcca aataataact agtactaagc aaagaattaa tcatggctga ccgactgtcc
      721 cgcaccaacg gtgagccttc agcgtgcgcc ttcttaaaag tctctagccc ggacatcatt
      781 tttccttgac taaactcaat aatgccgtca atagtcccat tcagcatttt aatagtggta
      841 atattagctg cccaaatgag ccaagccata agcaatccac cacccactag cacccaaatt
      901 tctttacttg tccaaccctg gtccttttct ttcttatctc cctctggccg atgatacaaa
      961 ttattaatta aaccaagaat aatcattaag aagatataac tcgccagaca atcaaaaaga
     1021 gcaagattct ggacaaaata ggaggccaga aaggctgcta aagcggcaaa ctctggcagt
     1081 ttaattttat cttcccggta ggccttaatt aaataaatca ctgcccaggt gaacataaat
     1141 aaataggccg ccagccctaa cagcccagta gtagtagcaa tctccaccac attattatgg
     1201 gctcggtcaa aataatcttc acccttgcca aaacgataga aatcaccctc aaattggcgg
     1261 tcaaagacta aagcataatt gccataaccg actcctaaga gtggatggct ccctaaatca
     1321 cgccaggccc atttccagga ataaaatcta gtatttaaag taggattctc cagagaaaaa
     1381 tcgcggataa tcttgcctaa ttgggtatta tctaaggccg gtgaccagcg gaaagcaaag
     1441 agcaggccca cccctataac cagaacacca accacaacca acccccactg ccggactttc
     1501 ttcttatgat ttaaaaaagt catcaggccg gcataaacta aaataccaac cgctagccca
     1561 gcataagctc cagagatatc cgccctgacg aaaccaacaa agagcaaaat aaggactaac
     1621 caatagggaa gcgacactaa ccaattacga ctccggctaa taagcaaagc agtaatgaac
     1681 atggtcatga gcattaaggt gcccatatag gccgcgttac ctaaggtgga agcattataa
     1741 ttttgaaaaa gtccaattag actaagcata gtgccggcac caaccatggc cataagcaga
     1801 ggctgccagt cccgccagtt gcgaaaaacc gtaataacaa taaggtataa caaaaagaaa
     1861 tgaaacaaag gaaaccagcc cagcatccgc tctaaatcgc cccaaaagct taaattaaaa
     1921 tctaccccag taaaacaggt aataaggaca ataagcaaat agaccgccca gccaatagtc
     1981 agccatgatt tcttaggccg ttcgctcgga tatttaataa tataggctag ccagacaaca
     2041 gttaaaatct ctattaagat attaaaatac agctgtttag aagtaatata cggaaaatag
     2101 aaatgtttat taactaaaaa taaagaaact aggctggcat aaacgcccac ctttaaaata
     2161 aagcggtacc a
//
LOCUS       DAOOIS010000047         2104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_41726_length_2104_cov_72.387018, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000047 DAOOIS010000000
VERSION     DAOOIS010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41726_length_2104_cov_72.387018"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1702
                     /locus_tag="PK960_02885"
     CDS             <1..1702
                     /locus_tag="PK960_02885"
                     /inference="COORDINATES: protein motif:HMM:NF023671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arabinofuranosyltransferase"
                     /protein_id="HPD08139.1"
                     /translation="SFMNFSDIQIYSSIKIILMLFAILAAYLIIKKKNPLYLLLLFGL
                     TSAGTYALWSNNLQLPLWGLQGDELTIAAIFNTLAKIGFSSDFSYHGLPPFYPPGSFW
                     FFSFWGRLFNWNGIIMMKVAATTFFLLFPAVVFLLQRYLFKNILPNHRLNVLLATLSP
                     LLIMTVLDKDLIIGKPYEIVAAVATILWTISLYHRVNNQRWTIWQPLIYGLAAGLIFM
                     TYYLWLAFAAIALIIMGLTEKNNHRLRYFWRLTQTAIVSFIATLPFTGPLLSSYLKNG
                     LESWQTALFTPTGLNLWLPMFQLTSWTNLIFLFGLGALIYYHRDPIARQLLYLFSTAF
                     IWWGGGLLTLLIWHKPFQEFRGFYIWAPTILAMGAAYGLSRIWQHYNLDQKTKTAITL
                     ALLGLGLLIAQSFFGFFIDDPTIRNQRIKSKQMDPSIVQLSQYLNNHPLPQDSLTLET
                     VPQLLAIVPINNLIYFNQHNNHPAAIFSKRYNYVQDLATAKSPAELIQKINNCPFGPL
                     ERFIFYGDQENYYLYFHVDKFISGLEEKTIKFNRQLFVPPYFQVNYNNLGYYVIDVQK
                     "
     gene            1680..>2104
                     /gene="tsaE"
                     /locus_tag="PK960_02890"
     CDS             1680..>2104
                     /gene="tsaE"
                     /locus_tag="PK960_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPD08140.1"
                     /translation="MSLTSKNKQSTNSYYAPTPRQTASLARRLAKNWHGGEIIALSGE
                     LGTGKTFFTQAVAKYLGVTTPVTSPTFVICEHYSCDHPTIKELIHIDAYRLNSSNDLI
                     NLGFSDFAGQPGYLTIIEWPEKIADLLPANTWHIHLDHQP"
BASE COUNT          589 a          479 c          349 g          687 t
ORIGIN      
        1 aagctttatg aatttttctg atatccaaat ttatagcagc atcaaaataa ttttgatgct
       61 ttttgctatt ttagcggctt acttaattat taagaaaaaa aaccccctgt accttctgtt
      121 gctttttgga cttacctcag ctggtactta tgccttatgg tctaataatt tacagctgcc
      181 tttatggggt cttcagggtg atgagctaac tattgccgct atatttaata ctttagctaa
      241 aattggcttc agctccgact ttagttatca tggcctgcct cctttctatc caccgggtag
      301 tttttggttt tttagtttct ggggaagact ctttaattgg aatggcatca taatgatgaa
      361 agtggctgct accaccttct tcttgctttt cccagcagta gtctttctac tacaacgtta
      421 tctatttaaa aatatcctcc ctaaccatcg cctcaatgta ctactggcca ccctgtctcc
      481 tttactaatt atgactgttc tagacaagga cctaatcatt ggcaaacctt atgaaatagt
      541 agctgccgtg gctacaattc tatggactat tagcctatac caccgagtaa acaatcaacg
      601 ctggaccatc tggcagccgt taatttatgg attagctgcc ggcttaatat ttatgactta
      661 ttatctctgg ctggctttcg cagccattgc tttaataata atgggtctga cagagaaaaa
      721 taatcaccgg cttagatatt tttggcggct gacccaaacc gctatcgtat ctttcatcgc
      781 caccctgccc tttactggac cgctacttag tagctatctc aaaaatggct tggaaagttg
      841 gcaaactgcc ctttttaccc ctactggctt aaatctatgg ctgccgatgt tccaactaac
      901 aagctggact aatttaatct ttttgtttgg cctaggtgcc ttaatttatt atcatcgcga
      961 tcccatcgct aggcagctgc tttacttatt ctctacagct tttatctggt ggggtggtgg
     1021 cttgctaact ctgttaattt ggcataagcc atttcaagaa ttccgcggtt tttatatctg
     1081 ggcccccact atcttggcta tgggagcagc ctatggctta agccgcattt ggcaacatta
     1141 taacttagac caaaaaacta aaacagcaat aaccttagcc ttgcttggcc taggtctatt
     1201 aattgctcag tcattttttg gttttttcat cgacgaccca actatcagaa atcaacgaat
     1261 taaatctaaa caaatggacc catctattgt ccaattaagt caatatctta ataatcatcc
     1321 tttgcctcaa gatagtttaa ctctagaaac cgttcctcag ctattagcta ttgtcccaat
     1381 taataactta atttatttta atcagcataa taatcatcca gctgccatct tctctaaaag
     1441 gtataattat gtccaagatc tagccactgc caagtcgccc gctgaattaa tccaaaaaat
     1501 caacaattgt ccttttggac cgttagaacg cttcatcttc tacggcgacc aagaaaatta
     1561 ttacctttat tttcatgtag ataaattcat tagcggccta gaagaaaaaa ctattaaatt
     1621 taaccgccag ctgtttgtcc ctccatattt tcaagttaat tataataatc ttggctatta
     1681 tgtcattgac gtccaaaaat aaacagtcta ctaatagcta ttatgcgcca acgccccgac
     1741 aaacggctag cctagctcgc cgtttagcca aaaattggca tggcggagaa attattgccc
     1801 tatctggtga actggggaca ggaaaaactt tttttactca agcggtagct aaatatttag
     1861 gggttactac tccagtcacc agccccacct ttgttatctg tgaacattac tcctgtgacc
     1921 acccaacaat taaagagtta atccatatag acgcatacag attaaattca tctaacgact
     1981 tgattaattt aggcttcagc gattttgccg gtcagcctgg ctatttaaca attattgaat
     2041 ggccagaaaa aatagccgac ttactgccag ctaatacctg gcatattcat ctagatcatc
     2101 aacc
//
LOCUS       DAOOIS010000048         2071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_42689_length_2071_cov_51.645833, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000048 DAOOIS010000000
VERSION     DAOOIS010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42689_length_2071_cov_51.645833"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1388)
                     /locus_tag="PK960_02895"
     CDS             complement(93..1388)
                     /locus_tag="PK960_02895"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPD08141.1"
                     /translation="MANIFLHDEFNPEDTAMLQALYSRSPESVEKHTEKVKKTGSGKF
                     METFYIGYGHASIADCGSTTLFIEGLSILADKAIQDWPLYCGQETSTRYTDMTKQSII
                     DPLNTKESQQILKNWLDFYKSSLGPMREYLQQRYPRKAGQEDKIYERAINARAFDILR
                     SFLPAGITTQLSWHTNLRQAYDKITWLRAYPLPEVQQIASNLEQILKEHYPHSFCQPV
                     DEAAENYRRHILTHNYYYNPDQVVNFRFTTNIKDQDLEKWSYALTNRPPKTSLPYFLS
                     ELGQCTFEFLLDYGSFRDIQRHRNGTCRIPLLTSQYGFEPWYLAELPSKLHDSALKLI
                     DQQIKAIANLPADKPTRQYYLALGFLVPCKVTYGLPATVYTAELRSGTTVHPTLRAIA
                     HQMIRALQDHWPQLKLYADFTPNEWDINRGKQTIEEKNN"
     gene            1510..>2071
                     /locus_tag="PK960_02900"
     CDS             1510..>2071
                     /locus_tag="PK960_02900"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPD08142.1"
                     /translation="MAWWQALILGATEGITEFLPISSTAHLRLFSEWLGIVNSDFSKS
                     FDIFIQLGAILAVLAVYLKTLYRQPKIILKLIAAFIPTGIIGLLAYPLVKSQFMDSFW
                     LIASALGLGGLFLIWFERYYHKKKVKSGGLEQISYQQACLIGLCQALAIIPGISRSAA
                     TIVGGLALGLKRSTIVEFSFLLALPTM"
BASE COUNT          579 a          356 c          462 g          674 t
ORIGIN      
        1 gccctgtcac ggcagaggtc acgggttcga ggcccgtcgg ctccgcaaga caccctctag
       61 gcagggtgtt ttttgcatat tttaatatag ttttaattgt ttttttcttc aatagtttgc
      121 ttaccacggt taatatccca ttcatttgga gtaaagtcag cataaagttt aagctgaggc
      181 cagtggtctt gtagtgctct aatcatctgg tgggctatgg cccgaagagt aggatgaacc
      241 gttgttcctg aacgcagttc agctgtatag acagtagctg gcaggccata ggtgacctta
      301 caagggacga gaaagcctag ggctaaataa tactgtctag ttggcttatc ggccggtaaa
      361 ttagcaatag ctttaatttg ttgatcaatt aattttaaag ccgagtcatg tagttttgac
      421 ggtagttctg ctaaatacca tggttcaaaa ccatactgag aagtaagtag gggaatacgg
      481 caagtgccat tacgatgacg ttgaatatcg cggaaagacc catagtctaa aagaaactca
      541 aaagtgcatt gtcctagttc agataaaaaa taaggcaaac tagttttagg cggacgatta
      601 gttagggcat aagaccattt ttctaaatct tggtctttaa tgttagtggt aaaacgaaaa
      661 ttaactacct gatcaggatt ataataatag ttgtgagtta aaatatggcg gcgataattt
      721 tctgccgctt catccaccgg ctgacagaaa ctatgaggat aatgctcttt tagaatttgc
      781 tcaagattgc tagcaatttg ttggacttcg ggtagggggt aagcccgaag ccaggtaatt
      841 ttatcatagg cttgccgtaa attagtatgc caacttaatt gagtggtaat accagccggc
      901 agaaaagagc gaaggatatc aaaagctcga gcattaatgg ctcgctcata gattttgtct
      961 tcttgaccag ccttacgagg atatctttgt tgtaaatatt ctctcatggg ccctaaggat
     1021 gatttataga aatctagcca atttttaagg atttgttgcg attccttggt gtttaatgga
     1081 tcaataatcg actgtttggt catatcagta tagcgggtgc tagtttcttg accacagtag
     1141 agtggccaat cttgaattgc cttgtcggct aaaatgctta aaccttcaat aaaaagagta
     1201 gtactgccac agtcagcaat gctagcatgg ccgtagccaa tgtagaaagt ttccatgaat
     1261 ttacctgagc cagttttttt aactttctcg gtgtgttttt ctacgctttc agggcttcgc
     1321 gaataaagcg cctggagcat ggcggtatct tctggattaa actcatcatg aaggaaaata
     1381 ttagccatag aattaaaatt attagattat attggcattg tatcaaggct gagcgagcca
     1441 gtcaaaaact tggcggataa taaaaattgc tttacaatat agatatcttt atatttgatt
     1501 tttattttta tggcctggtg gcaagcccta attttgggag ctacagaggg gattactgag
     1561 tttttaccca tttcttctac tgctcatctt cgtttattta gtgaatggtt gggtattgtt
     1621 aattctgatt ttagcaaaag ttttgatatt tttattcaat tgggggctat tttagccgtg
     1681 ctggcagttt atttaaaaac attatatcgg caacctaaga ttattttaaa attaattgcc
     1741 gcttttattc ctactggcat tattggactg ttagcttatc ctttagttaa aagtcagttt
     1801 atggatagtt tttggctgat tgcttctgcc ttggggctgg gagggttatt cttaatatgg
     1861 tttgagaggt attaccacaa gaaaaaagta aaaagcggag gactagaaca gatatcatat
     1921 caacaagctt gtcttatcgg gttatgtcaa gccctagcca ttatcccggg catttctcgg
     1981 tcggcggcta ccattgtcgg tggtttagct ctaggattaa agagatcaac catagtagag
     2041 tttagttttt tattggcttt acccaccatg t
//
LOCUS       DAOOIS010000049         1990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_45258_length_1990_cov_55.016021, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000049 DAOOIS010000000
VERSION     DAOOIS010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45258_length_1990_cov_55.016021"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /gene="secD"
                     /locus_tag="PK960_02905"
     CDS             <1..983
                     /gene="secD"
                     /locus_tag="PK960_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPD08143.1"
                     /translation="IYKEDERPMMDYKLSHILIKTKSIVDILGNVDEWKTTELTGKYL
                     KRATVQFNPQDNTPEVALEFDSEGAKLFEDITGRNINKPVAIFLDGYMISQPTVNEKI
                     TGGQAVISGRFSLAEAKTLAQRLNAGALPVPITLVNQQTIGASLGQSSVNKSLTAGIV
                     GLILVALFMIIVYRLPGLLSVIALLVYGSLTLSIFKVWPVTLTLSGIAGFILSIGMAV
                     DANVLIFERMKEELRAGKPLGTAINEGFSRAWPSIRDGNLTTLIICFILIQFLTGSIK
                     GFAITLALGVLMSMFSAVVVTRNLLKLVVTPKIASKLWLFGVKPASDKQA"
     gene            1006..1920
                     /gene="secF"
                     /locus_tag="PK960_02910"
     CDS             1006..1920
                     /gene="secF"
                     /locus_tag="PK960_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPD08144.1"
                     /translation="MYQFIPKRKIWLTISAVLIALSIAAVAFWGLSFGIDFTGGSVLE
                     VRYSQNRPSVTAINESLSSLGLQNLIVQPVGDTDVKLRFQEVDEAKHQAILAKLQELA
                     PAGEGQSSMEELRYDSVGPTVGRDLQTRAVTSIILSLIAIVIYIAWAFHKVSQPVSSW
                     KYGVCAILALVHDVIIIIGFFAVMGHYFGYQLDITSIAVILTVLGYSVNDTIVVFDRT
                     RENLFRLRQGFAETVNISLNQTLTRSLYTSFTTILALMAIVIFGGSSIRDFALALTVG
                     VFFGTYSSIFVASPLLVIWNNLHNRKKA"
BASE COUNT          510 a          336 c          438 g          706 t
ORIGIN      
        1 taatttataa agaagatgaa cggccgatga tggattataa gttaagccat attttgatta
       61 aaactaaatc tattgtggat attctcggta atgttgatga gtggaaaacg actgagctta
      121 ctggtaaata tttaaaacga gctacggtac aatttaatcc tcaagataat actccagaag
      181 tagcactaga gtttgatagt gaaggggcca agttatttga agatattacg ggacgcaata
      241 ttaataagcc ggtagctatt tttctagatg gttatatgat tagtcagccg acagttaatg
      301 aaaaaataac cggtgggcag gcagttattt ctggccgatt tagtttagcg gaggctaaaa
      361 ctttagccca acgtcttaat gctggcgctt tgcccgtacc aattacttta gttaatcaac
      421 aaactatcgg agctagtctg ggccaatcat cggtgaataa aagtttaact gctggaatag
      481 ttggtcttat tttagtagct ttatttatga ttattgttta ccgcttgcca gggttgctgt
      541 cagtgatcgc tttgttggtt tatggttcat tgactttatc tatctttaag gtatggccag
      601 ttacccttac tctttcaggg attgccggtt ttattttgtc cattggtatg gccgttgacg
      661 ctaatgtgct tatttttgaa agaatgaagg aagaattaag ggcggggaaa ccattaggaa
      721 cagctattaa cgaaggcttt tcccgggcct ggccgtcaat tcgcgatggt aatcttacca
      781 cgcttattat ttgttttatt ttgatccaat ttttgaccgg ctccattaag gggtttgcta
      841 taaccttggc tttgggtgtg ttaatgtcta tgtttagcgc ggttgttgtt actcgtaatt
      901 tattaaaatt agtggtgact cccaagatag ctagcaaact atggcttttt ggtgttaagc
      961 cagcgtcaga taaacaagct taaaatttat taactattga attatatgta tcagtttatt
     1021 cctaaaagaa aaatttggtt gactatctct gctgttttga tagctttgtc tatagcggcc
     1081 gttgctttct ggggtttatc atttggtatt gattttaccg gcggtagcgt tttagaagtg
     1141 cgttattcgc agaatcgtcc cagcgtaacc gccattaatg agagtttgtc tagcttaggt
     1201 ttacagaatt taattgtcca gccagtaggg gatactgatg tgaaacttcg attccaggag
     1261 gtagatgagg ctaaacatca ggctattctt gctaagctac aagaattagc gccggcaggt
     1321 gaagggcaaa gttctatgga ggagttgcgg tatgattctg tcgggccaac agtaggtcgt
     1381 gatttacaga ccagagccgt gacctcaatt attttgtctt taatagctat tgtgatttat
     1441 attgcctggg cctttcataa ggtttctcaa ccagtgagct cctggaaata tggggtgtgc
     1501 gctattttag ccttggtcca cgatgttatt attataattg gtttttttgc tgttatggga
     1561 cactattttg gctatcagct agacattact tctattgctg ttattttgac ggtgttaggc
     1621 tatagtgtga atgatactat tgttgttttt gaccgcactc gagaaaattt atttcgtctc
     1681 cggcagggct ttgccgagac ggttaatatt agcttaaatc aaacattaac tcgttcccta
     1741 tatacttctt ttactaccat tctggctttg atggctattg ttatttttgg tggttcatca
     1801 attcgtgatt ttgccttagc cctaactgtc ggtgtctttt ttggtactta tagttccatc
     1861 tttgttgcct cgcctctttt ggtaatctgg aataatttac ataatcgtaa aaaagcttaa
     1921 tttttatatt attatctatg cgccaaagcc agctttttac ccacagcttg aaggagctcc
     1981 ctaaagatga
//
LOCUS       DAOOIS010000050         1982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_45531_length_1982_cov_33.095485, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000050 DAOOIS010000000
VERSION     DAOOIS010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45531_length_1982_cov_33.095485"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..1296
                     /locus_tag="PK960_02915"
     CDS             133..1296
                     /locus_tag="PK960_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPD08145.1"
                     /translation="MAHGDYESEINELNSQIDKKKDALSKLRDQQRYYQQLIAEKQQE
                     KASLKNQLSILDNKMAEIELDIEGTRLEMEQTELELRSLELEIANKDQQIDQAKDRLS
                     KLIKTIDQQGSRNPLEVILMNDSFSDFLNDIEYLNNISSAVRDELEELKDLKASLEEQ
                     QQTLNNKREQLAELQRVLADKKDSLDQEKQNKNFLLEQTQLSESQYQTLLADAKKEQE
                     QANAEISALEKTVRQKLAQLEAKRPKNNQNESNNTSVFIWPVPKNRITAYFHDPDYPF
                     RDVFEHPAIDIKAGQGTPIMAVADGYIAAAKDAGMGYSYIMIVHDNGLATVYGHVSKI
                     VVKEEDYVTAGQLIGYSGGTPGTPGAGKMTTGPHLHFEVRLNGIPVDPLAYLP"
     gene            1301..1867
                     /locus_tag="PK960_02920"
     CDS             1301..1867
                     /locus_tag="PK960_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08146.1"
                     /translation="MKKTNHYYLPGFGPLSAIFILSLLLLIASAGFTYWSINQAKERD
                     QIRIANLKTIQSAINQYYNQYGEFPKGDKDIGNWDLGYQNSDDHRFIYPLVEKGFLPP
                     NFPADPISKYDPTHGIDYAFHYQVYADDGQFCPRGQGEFYVLGIRDLESSTSSPAEGG
                     GFSCKNRNWQDEFDYVVGAFAHPAKNKQ"
BASE COUNT          678 a          377 c          385 g          542 t
ORIGIN      
        1 atggtcctag tcaaagtttt taaaaaatag tataattgag ccatgaagca acatcatcgt
       61 gtttacaata taataaaggg cagtttgaca ctaatagtga tcttttcttt gcttattatc
      121 aacagagcac caatggcgca tggtgattat gaaagtgaaa ttaatgagct aaatagccaa
      181 atagataaaa agaaagatgc ccttagtaaa ttacgcgatc aacagcgtta ctaccaacag
      241 cttattgctg aaaaacagca ggaaaaggct agtcttaaaa accaactgtc tattttggat
      301 aacaaaatgg ccgaaattga gctagacatt gagggcacta ggctagaaat ggagcaaaca
      361 gaactagaac tacgcagttt agaattggaa attgccaata aagaccaaca aattgatcaa
      421 gctaaagatc gattaagtaa actcattaag actattgacc agcaggggag taggaaccca
      481 ttagaggtta ttctaatgaa tgattccttt tctgatttcc ttaatgatat tgaatatctc
      541 aacaatatta gcagtgcagt ccgcgacgaa ctggaagagt taaaagacct caaagcttca
      601 ctagaagaac agcaacaaac cttaaataat aaacgtgagc agttagcgga attacagcga
      661 gtgttggcgg acaaaaaaga tagtctagac caagaaaaac aaaataagaa ttttctcttg
      721 gaacaaactc aattatctga aagtcaatat caaacactat tagctgatgc taaaaaagag
      781 caagaacagg ctaatgcaga aattagcgca ttggaaaaga cggtccgtca aaaattagct
      841 cagctagaag ccaagaggcc taagaataat caaaatgaat caaataatac ctcagttttt
      901 atctggccag tgcccaaaaa tcgtattact gcttattttc atgacccaga ttatcctttc
      961 cgcgatgttt ttgagcatcc agcaatagat attaaagccg ggcaggggac tcccattatg
     1021 gcagtagctg atggctacat agcggcagct aaagatgctg gcatgggtta tagctatatt
     1081 atgattgttc atgataatgg actggcgact gtctatggcc atgtctctaa aatagtagta
     1141 aaagaagagg actatgttac tgctggccaa ctaattggct atagcggagg cactcccggc
     1201 acccctggcg ctggtaagat gactactggc cctcatttac actttgaagt ccgtttaaat
     1261 ggcattcccg tagacccatt agcttattta ccataaccac atgaaaaaga ctaatcacta
     1321 ctacttacct ggctttggac ccttgtccgc tatctttatc ttaagccttc tccttctaat
     1381 agctagcgct ggttttactt attggtctat taaccaagct aaagagcggg accaaataag
     1441 aattgccaat ttaaaaacaa tccagtcagc cattaatcaa tattataatc aatatggtga
     1501 attcccaaaa ggagataaag atattggcaa ctgggattta ggctaccaaa atagtgatga
     1561 ccatcgtttt atttatccct tagtggaaaa gggctttcta ccgcctaatt ttcctgctga
     1621 tccaatttct aaatatgatc ccacccacgg cattgattat gctttccact accaagttta
     1681 tgctgatgat ggccaatttt gcccgcgtgg ccagggcgag ttctatgttt taggtatccg
     1741 tgaccttgaa tcatcaacca gcagtcctgc tgaagggggt ggttttagct gtaaaaatcg
     1801 taactggcaa gatgaatttg attatgtcgt tggtgctttt gctcatccgg ctaaaaataa
     1861 acaatagtag taaaaaagca taataaaata aaaaaagaaa tcagacggat ttcttttttt
     1921 atttgctaca agtcttaacg gggagaatga tatacaacgt cgcacaatac atcttatacg
     1981 ac
//
LOCUS       DAOOIS010000051         1968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_46005_length_1968_cov_59.542081, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000051 DAOOIS010000000
VERSION     DAOOIS010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46005_length_1968_cov_59.542081"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..652
                     /locus_tag="PK960_02925"
     CDS             80..652
                     /locus_tag="PK960_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08147.1"
                     /translation="METPLKTVNTQNLASLLIWQICQEYKNTLNKKEYWEARLQKMVR
                     IKKAFFHISLFLVYLLVAYWLMSLAPSPSPTIFLDYVSGSIIGMAVISCLALLWAKQR
                     VGHYQKRVGEINKGVVEFIKKRLNQILLSHTCFAHDDLDVVIDFYYKQLSCIVGDFFK
                     ESFTSPLSHEEEEEIRSWLKTSWPNFFISP"
     gene            728..1090
                     /locus_tag="PK960_02930"
     CDS             728..1090
                     /locus_tag="PK960_02930"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPD08148.1"
                     /translation="MPDTSQFAALVYLATSQIPLGRISTYGAIAQAIGRPQASRAVGQ
                     ALHHNPFAPTVPCHRVVSHSGKLIGFIQGVEAQKNLLTKEGIMVDNQGKIMNFNKVFY
                     QPKICPQAIWKLSKNYRP"
     gene            1045..1767
                     /locus_tag="PK960_02935"
     CDS             1045..1767
                     /locus_tag="PK960_02935"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPD08149.1"
                     /translation="MSPSYLEIIQKLQALANANKAKTLSQFFKTGAGDYGAYDIFWGI
                     PVPLQRPLAQEYYNLPLRDIKKLLTHPVHEVRFTGALILVNHYNRSDKKEQKNTLDFY
                     LNNIHLFNSWDIIDLTAPKILGEYLVNNKNYNILSTLAKVDNLWAKRAAMVANLTLIK
                     KGECGPALKLAKQYLSQKEELLHKATGWMLREIGKQNQSSLLNFLDQYASQMPRIMLR
                     YSLEKLPPELREKYLKRRYELH"
     gene            1754..>1968
                     /locus_tag="PK960_02940"
     CDS             1754..>1968
                     /locus_tag="PK960_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD08150.1"
                     /translation="MNFINLMGVRLTNLNNHELWTIIESYLQSSHNHLIVTANPEFIL
                     TAQNDKKFRDILNSADLALPDGVGLILA"
BASE COUNT          655 a          360 c          366 g          587 t
ORIGIN      
        1 gttgacaaat aattgactta ggactagggt aatatatcta taaaatgtac ctaaaaaata
       61 aaaaaggggg gtgatcatca tggaaacacc gctcaaaacc gtcaataccc aaaatttggc
      121 tagcctcctc atttggcaaa tttgccaaga atacaaaaat actctaaata aaaaagagta
      181 ttgggaggct aggctacaaa aaatggtgag gattaaaaaa gcgtttttcc acataagttt
      241 atttctcgtc tatttgttgg tagcctattg gcttatgagt ttagctccta gccccagtcc
      301 tacaatattc ttagattatg tttctgggag tataatagga atggcggtaa ttagctgtct
      361 agcacttctt tgggcaaaac aaagagtggg tcactatcaa aaacgagttg gagaaattaa
      421 caagggagtg gtggaattta ttaaaaaacg ccttaatcaa attctactgt cccacacttg
      481 ttttgctcac gatgacctgg atgtcgttat tgatttttat tacaagcagc tcagctgcat
      541 tgtgggtgat tttttcaaag aaagttttac ctctcctctt agccatgagg aggaagagga
      601 aataagaagc tggctcaaaa ccagctggcc caattttttt attagcccct gagcccaggg
      661 gcttttttat tgccgataac ttcaattatt tgtataataa actcatctta aatgttgtaa
      721 aaagtgtatg ccagatacca gtcaatttgc agctttagtt tatctagcta ccagtcaaat
      781 tcctttgggt cgcattagta cttacggtgc cattgctcaa gctattggca ggcctcaggc
      841 cagccgggca gtcggccaag cgctccacca caatcctttt gccccaactg tgccttgcca
      901 tcgtgtagta agccatagcg gtaagttaat tggctttatt cagggcgtag aagctcaaaa
      961 gaatcttcta actaaagagg ggattatggt tgataaccaa ggcaaaataa tgaattttaa
     1021 taaagtattt taccagccta aaatatgtcc ccaagctatc tggaaattat ccaaaaatta
     1081 caggccttag ctaacgctaa taaagctaaa actttgagcc aattttttaa aactggtgcc
     1141 ggggattatg gggcttatga tattttttgg ggcataccag taccactaca acggcccctg
     1201 gctcaagaat attataattt accactaaga gatattaaaa aattattaac tcatccagtg
     1261 catgaagtaa ggtttactgg tgctcttatt ttggttaatc attataatcg aagtgataaa
     1321 aaagaacaaa agaatacttt agatttttat ctaaataata ttcatctctt taatagctgg
     1381 gatattattg acttaactgc tccaaaaatt cttggtgaat atttagttaa taataaaaat
     1441 tataatatcc tatcaaccct ggctaaagtt gataatttat gggctaaacg agctgccatg
     1501 gtcgctaatc taaccttaat taaaaaaggg gaatgtggtc cggccctaaa actagccaag
     1561 caatatttat ctcaaaaaga agaactactg cataaagcaa ccggctggat gttacgagaa
     1621 attggtaagc agaatcaatc atcgctcctg aactttttag accagtatgc tagtcaaatg
     1681 ccacgtatca tgttaagata tagtttagaa aaactgccac cagaactaag agaaaaatat
     1741 ttaaaaagac gttatgaact tcattaattt aatgggtgtg cggctgacca acctcaataa
     1801 ccacgaacta tggactatta ttgaaagcta cctccaaagt agtcataatc atttaattgt
     1861 cactgctaat cccgagttta tattaacagc ccaaaatgat aaaaaattta gagatatttt
     1921 aaatagcgct gacctagctt tacccgatgg agttggctta attctggc
//
LOCUS       DAOOIS010000052         1901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_48401_length_1901_cov_42.290899, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000052 DAOOIS010000000
VERSION     DAOOIS010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48401_length_1901_cov_42.290899"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1901)
                     /locus_tag="PK960_02945"
     CDS             complement(<1..>1901)
                     /locus_tag="PK960_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08151.1"
                     /translation="VVAGTVTAFSSAGKSAAQASARALGGLGLGAILTGGLAAARESQ
                     MLKQERKQHSREKAQGKEFSEKDERRAEMEKFIYETKRAQDILNSLNINFTELGPDNL
                     EPLHQAINSLADLEARIRLSDQLTIDLIAFSSIVAVERERLALDLARAQLKTTLRRLV
                     NNKKIDFAGNNFDDYLSTLTEAKVQELIGGDQGIREKDRLFQKMRHQRMAKTGVKAAA
                     TGLAVGLVFQEVTSFFRDDQQGLIEGLVKGDKADSTQTMTVLEKARTMIFGKPQLDQA
                     QEIVKVGNQQFKVPVGTEIKLETDGDYSIYYNGRQLVGDISLTPEGQLTAESQAALAK
                     AGIDWIPQQNQIAGTPGARDIINSHGEETVSIDRSLWYNNDTPHIFDKNELKLLLGGI
                     NGSGLDADGNFVFNVSRMTPDGSFHTLSSGQVLSADAPDLIKKGLLKFFISVSKGTQN
                     QVFELPIQPNGTVVIPPDSFLASLFTVSANGTVQFEGQYMEVAQTMGISNGKEVVRIL
                     ATQVGKGLSESSTFITNVVHNIGIHLANLWEMSPIVPLFGRRPLEPAKEIKKFHKPEY
                     AGYYGGETSAAEKEEYERYLSPRLKADKKAKLNLQKELAWVFEEKKAREPGQVEKVDR
                     IIKQDKLLV"
BASE COUNT          529 a          457 c          315 g          600 t
ORIGIN      
        1 tcaactaata atttatcttg tttaataatt ctatcaactt tttctacttg ccctggctct
       61 ctagcctttt tttcttcaaa tacccaggct agttcttttt gaagatttag tttggccttc
      121 ttatccgcct ttagacgagg tgataaatat ctttcatatt cttctttttc ggcagcagat
      181 gtctcacctc cataataacc agcgtattca ggtttatgaa attttttaat ttctttcgct
      241 ggctctaacg gccgacggcc aaacaatgga actattggtg acatttccca aagattagca
      301 aggtggatac caatattatg taccacattg gttataaacg tagagctctc agatagcccc
      361 ttaccaactt gagtagccag aatacgcact acttcttttc cgttggatat ccccatcgtt
      421 tgtgcgacct ccatatattg accttcaaat tgaacagtgc cgttagcaga aacagtaaac
      481 aaactagcaa gaaaactatc agggggaatt accactgttc catttggttg tattggtagc
      541 tcaaaaacct gattctgtgt tcctttagac acagatataa aaaatttaag caaacctttc
      601 tttattagat ctggggcatc tgctgacaat acctgaccgc tacttaaagt atgaaaagat
      661 ccgtcgggtg tcatgcgact cacattaaaa acaaaattgc catctgcgtc taacccacta
      721 ccattaattc cacccaggag tagctttaat tcattcttgt caaaaatgtg gggggtatca
      781 ttattatacc aaagcgatcg atcaatagat acagtctcct ccccatgaga gttaatgatg
      841 tctctagcgc caggggttcc ggcaatttga ttttgctggg gtatccaatc aatacctgcc
      901 ttagctaaag cggcttgtga ttcggccgtc aactgtccct ccggggttaa acttatatca
      961 cccaccaatt gacgaccatt ataataaatg ctgtaatcac catctgtttc aagcttgatc
     1021 tctgttccta ctggcacctt aaactgctga ttgccaactt tgactatttc ttgagcctga
     1081 tcgagctgtg gcttaccaaa aatcatggtc ctcgccttct ccaggacagt cattgtctga
     1141 gtactatcag ctttatcacc cttaactaat ccctcaataa gtccctgctg atcatcacgg
     1201 aaaaaactag taacctcctg gaacaccaaa ccaactgcta ggccagtagc ggccgctttg
     1261 actcctgtct tagccattct ttgatgacgc attttttgaa aaagacgatc tttctcccta
     1321 ataccttgat caccacctat taattcctga accttagctt ctgttaatgt acttaaataa
     1381 tcatcaaaat tattaccagc aaaatcaatc ttcttattat taactagtcg tcgtaatgta
     1441 gttttcaatt gagcccgtgc taaatctaaa gctaaacgtt ctcgttctac tgcaactatg
     1501 ctagaaaagg caattaaatc aattgtcagc tggtcagata aacgaattct cgcctctaag
     1561 tcggctaaac tattaatagc ttgatgtaat ggctctaaat tatctggacc aagctcagtg
     1621 aagttaatat taagcgaatt caatatatct tgagctcgtt ttgtttcata gataaatttc
     1681 tccatttcag cccgacgctc atctttttca gaaaattctt tgccctgagc tttttcacga
     1741 ctgtgctgct tacgttcttg tttaagcatc tgtgattctc ttgccgctgc taatccacca
     1801 gtcagtatag caccaagccc taaaccaccc aaagcccgag cactagcctg agccgcactt
     1861 ttgccagcac tggaaaaagc agttactgtt ccagccacta c
//
LOCUS       DAOOIS010000053         1839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_50874_length_1839_cov_56.593049, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000053 DAOOIS010000000
VERSION     DAOOIS010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50874_length_1839_cov_56.593049"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="PK960_02950"
     CDS             <1..1251
                     /locus_tag="PK960_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08152.1"
                     /translation="AASPQAIVQHNTINYNNIGLEIAARGATISKNAIAYNNQAGLIL
                     LNASNVISDNNIYGNLGSDLSSTLPGGIVTNNYWGLGATINSSVPASSTFNFEPRLSE
                     PTDIASLSRPTVNETVKSTILEKILITGTKDANGNLIINDETVIPWDNEVTWSYNLPL
                     TLGDNTFTILSRDRAGNSSPAIEFTITRLENKFVPTPTINSFKLTTSEATQVLSGGKD
                     ANTAIIINGQEVVPLNNETKWQATVALRYGRNTFSIVARSAAGYESDPLSITIDRPDT
                     QGVETIAREKAASAGIPVNEALTKRLAGYILLQVESKGEAWYVNPLDGQRYFLAGPTN
                     AFNLMRQFGLGVKHSVITAKTIDPKLKGRILIDTEDSGKAYYVHPQTSKLYYLGRPAD
                     ALAVMRQVGLGISNKDLRQIKLAQ"
     gene            1375..1653
                     /locus_tag="PK960_02955"
     CDS             1375..1653
                     /locus_tag="PK960_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPD08153.1"
                     /translation="MSMTKAQLVEGLAKNCKLSKKAVNDVLNEMAAMAYKEVKRNGEF
                     IVPGIGKLVRMQRKARTGRNPATGAQIRIPAKNVVRFRVAKAVKDAVV"
BASE COUNT          522 a          368 c          413 g          536 t
ORIGIN      
        1 gctgcctcgc ctcaggctat cgtccagcat aatactataa attataataa tattggctta
       61 gaaatagcgg cgcggggggc aaccattagc aagaatgcga tagcctataa taatcaggca
      121 ggtttaattt tacttaatgc tagtaatgtc atcagcgaca ataacatcta tggcaattta
      181 ggctcagact tatctagtac tttaccggga ggtattgtga ctaataatta ttggggctta
      241 ggagcaacta taaatagcag tgtgcccgct agctctacct ttaattttga accacgattg
      301 agtgagccga ccgatattgc cagtctctct cgcccgacag tgaatgagac agtgaaatct
      361 actattttag agaagatcct tataactggt actaaagatg ccaacggcaa tttaattatt
      421 aatgatgaga ccgttattcc ttgggataat gaagttacct ggagttataa tctccctctt
      481 actttgggag ataatacttt taccatactt tcccgcgacc gggctggcaa tagtagtccg
      541 gcaatagagt ttactattac ccgcctagag aataaatttg tgcctacgcc aaccattaat
      601 agttttaaat taactacaag tgaggctacc caggtattaa gcgggggcaa ggatgctaat
      661 accgccatta ttattaatgg gcaggaagtc gtgccgctta ataatgagac taaatggcag
      721 gccactgtgg ctctgcgtta tggccgtaac actttttcca ttgtggctcg cagtgccgct
      781 ggctatgaaa gcgatccgct ttccattact attgaccggc cagatacgca gggggtggag
      841 actattgccc gcgagaaagc agcttcagct ggtatacccg taaatgaggc tttaactaaa
      901 agattagccg gttatattct ccttcaagtg gaatctaagg gtgaggcttg gtatgttaat
      961 cccttagatg gacaacgtta ttttcttgct ggtcctacta atgcctttaa tttgatgcgt
     1021 cagtttggtt tgggagtaaa gcatagcgtt attacggcca agaccattga tccaaaactt
     1081 aagggtcgga ttttaattga tactgaagat agcggtaaag cctattatgt tcacccgcag
     1141 accagtaaat tatattatct cggccggccg gctgatgctc tagcagtgat gcgtcaggta
     1201 ggcctgggaa ttagtaacaa agatctacgc caaattaaat tagcccaata atattggcta
     1261 tttctcgttt tttcttgttt aacccttgtc tttattggga actcttgtca tctttttcat
     1321 aaaggtatat aattacaagg taaatggcag gttgctgcca gataataata gtctatgtcc
     1381 atgacaaagg ctcaattagt tgagggcttg gctaagaact gcaaacttag caagaaagcc
     1441 gtcaatgatg tcttgaacga gatggccgcc atggcctaca aggaagtcaa gaggaatggc
     1501 gaattcatcg ttccaggcat cggtaagcta gttagaatgc agcgcaaggc ccgcacaggc
     1561 cgcaatccag cgactggtgc ccagattaga atcccggcca agaatgtcgt tagattccgc
     1621 gtggccaaag cggttaagga tgcggtagtt tagttactgc cgtaagctgc tattaccaaa
     1681 attaccgacc tccatgaggg ggtcggtaat tttttattaa ctaattttat tgtcttattc
     1741 tatccaagtt tctacgtatt gtcctaggcc aaatttttta agtttattat ttagtttagt
     1801 gcgggacctt aaaaaattaa gaagagagcc aaaatcaat
//
LOCUS       DAOOIS010000054         1752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_54685_length_1752_cov_57.732469, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000054 DAOOIS010000000
VERSION     DAOOIS010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54685_length_1752_cov_57.732469"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PK960_02960"
     CDS             <1..618
                     /locus_tag="PK960_02960"
                     /inference="COORDINATES: protein motif:HMM:NF039823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HPD08154.1"
                     /translation="FSQPGIMVGTILTIIIVGGGLATYVVGRQAKPGDSLYFAKEIND
                     QVKLAMRFDREEQNKLKLAMAQERVTEMANLDKEADKEKAEAISKEFQATLNEVKEGL
                     TAINNTAPVVTRKKEAVSTPDNNQVKTTEDTTTNDATATTSLEDGAFSVATGQTKDGR
                     IEISEPVNVSARQMIEEAETLFANADYDGTIDKLQAASELLDSAK"
     gene            1024..>1752
                     /locus_tag="PK960_02965"
     CDS             1024..>1752
                     /locus_tag="PK960_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08155.1"
                     /translation="MNRISKMFFLGLAVVALAGFGKVGVTKAAAQPGSLIKMNGLSSV
                     YYLGQDGKRYVFPNQTTYFSWYKDFSGVVTISQSELESYPLGSNITTRPGTYLVKITT
                     NPKVYAVEPGGLLRAIPDEASAKALYGDNWAKRVVDVPDAFFTNYKMGTALAAGEIPA
                     GALVKNADNPDVYYFDGTNYRKIADEAAFNANRFRFDFVLTVSTAITASGNNITTNEF
                     GNPDSSATGQGTQPGQGTGLTVALS"
BASE COUNT          499 a          360 c          413 g          480 t
ORIGIN      
        1 tttagtcaac ccggcattat ggtcggcact attttgacca tcattatagt gggcggcggt
       61 ctggccactt atgtggtcgg ccgccaggcc aagccgggag attcattata ttttgccaaa
      121 gaaattaatg accaggtaaa attagcgatg cgttttgatc gggaggaaca aaataaatta
      181 aaattagcta tggcccaaga acgagttacc gaaatggcta atttagataa agaagcagat
      241 aaagaaaagg ctgaggccat tagtaaagag tttcaggcga ccttgaatga agttaaagag
      301 ggattaacgg cgattaataa tactgcgccg gtggttactc ggaaaaagga ggcggtatct
      361 acgccggaca ataatcaggt caagacaacc gaagatacta ctactaatga tgctacagct
      421 actacttcgc tggaagacgg agcgtttagt gtagcgaccg gtcaaaccaa agatggccgg
      481 attgagatta gtgaaccagt taatgtcagc gcccggcaga tgattgaaga agcggaaact
      541 ttatttgcta atgccgatta cgatggcacc atagataaat tacaagctgc tagtgaattg
      601 ttagatagtg ctaaataata ttgaatattt ctcgttcttt ttcatttatt tataacctct
      661 tgtcaggttt cgtcttctga ggtataatgt ccccaagaag gcgcgagagc agaatgattg
      721 tccggagaat gaagccaacc tacccctcct ggttggtttc atcctccgcg gtaattactc
      781 tgttcaagct tgacagagta gttaagtgtg aggcttagct taaagctaag aatcatgaca
      841 ggacctatta aaggtcgaat cggtcaggag ggccggtggt caacaactgt cgaggcactt
      901 gacaataaag cgcgccgcta aataactagc tcgtgtaggc gtagctagtt ttattttaga
      961 ggcgttaata cttaattaac tcgtccatta aatgataaag atttaatgga cctaaatgac
     1021 actatgaata gaatttccaa aatgtttttc ttgggtctag ccgtagttgc cttagccggg
     1081 ttcggcaaag ttggcgtcac taaagccgcc gctcagccgg gcagtttgat taagatgaac
     1141 ggattatctt ccgtttatta tcttggtcaa gacggcaagc gctatgtctt ccccaatcag
     1201 acaacctact tttcgtggta caaggatttc agcggtgtcg tgaccatttc tcaaagtgaa
     1261 ttagagtcct atcctttagg ctccaatatt actacgagac cgggcacgta cctagtgaaa
     1321 attaccacta atccaaaagt ttacgctgtt gaaccaggtg gacttttacg cgccatacca
     1381 gatgaggcat cagccaaagc tctgtatggc gataattggg cgaaacgcgt cgtggatgtt
     1441 cccgatgctt tcttcaccaa ttataaaatg ggcactgctt tagctgctgg cgagattccc
     1501 gctggcgctt tagttaagaa tgcagacaat ccggatgtct attactttga tggcacaaat
     1561 tatcgcaaga ttgctgatga ggcggccttc aatgctaatc gtttccgctt tgactttgtc
     1621 ttaactgtta gcactgcaat tactgcttcc ggcaataata ttaccactaa tgaattcggt
     1681 aatccggatt cctcagcaac tggacaaggc actcagcctg gacagggaac tggtcttact
     1741 gtggctttaa gc
//
LOCUS       DAOOIS010000055         1747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_54922_length_1747_cov_42.187943, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000055 DAOOIS010000000
VERSION     DAOOIS010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54922_length_1747_cov_42.187943"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..624)
                     /gene="rplJ"
                     /locus_tag="PK960_02970"
     CDS             complement(88..624)
                     /gene="rplJ"
                     /locus_tag="PK960_02970"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPD08156.1"
                     /translation="MPKTKQQKQAIVQELEAKLAQARAVIFTSFNALPVKESESLRQK
                     LIKEQGEFWAVKKTLLSRALESLPAAEERPNVKEFDGQVAVVFAYGDQIAPAKVIHDF
                     QKNDETNRLKFLGGLLDNHWLSAYEVEQLANLPSKLELEGRLVGAMQSPITGLVNVLA
                     GNLRGLLITLQQIASSKT"
     gene            complement(772..>1747)
                     /locus_tag="PK960_02975"
     CDS             complement(772..>1747)
                     /locus_tag="PK960_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08157.1"
                     /translation="SKVNFSDEDYETIANGIIKYKKSLIFGGDSLIDELGKNIDKFPR
                     SLHKKIAQQLIDSNNGQVVAKNLEKFSGLNHQEIAQKLIDKHYGWSLVDNLEKFSGLN
                     HQEIAQRLIDKDQGDVVAKNLEKFSGLNHQEIAQKLIDSGQGDVVADNLEKFSGLNHQ
                     EIAQKLIKAKQSDALLKNADKFSNVDFNKIAHQLINLGNKEGKFYALANNLYKLSGLD
                     KDIAMGIINYGYDYRVAENLQAFTPADHKAIISKLAKTKNVNTKKWLATDLKNSFGKI
                     PQSNKAVARQLAKLGYIEILAEYPEKFPELDSDLQKQIDNYKATHPNK"
BASE COUNT          504 a          331 c          271 g          641 t
ORIGIN      
        1 tagtgggaac ttcaacttgt tcctctgatt gattcatttc ttcggtcata taattaaata
       61 aattaattcc gttagacgga ttttttatta agttttgcta ctagctattt gctgtagagt
      121 tattaacaaa cctcgcaaat taccagctaa aacattaact aagccagtta ttggactttg
      181 catagctcct actagacgac cctctagttc taatttgctt ggcaagttag ctagctgttc
      241 aacttcataa gcacttaacc aatgattatc caataaacca cctaaaaatt ttagcctatt
      301 agtttcatca tttttttgga agtcatgaat gactttagct ggtgcaattt gatcaccata
      361 agcaaagact acagccactt gtccatcaaa ttctttaaca tttggccgct cttcagcagc
      421 tggtaaactc tccaatgctc tactaagtaa ggtttttttt actgcccaga attctccctg
      481 ttccttaatt aatttttgcc gcaaagattc actttcctta accggtaaag cattaaatga
      541 ggtaaaaata actgccctgg cttgagctag tttagcttct aactcctgta caattgcttg
      601 tttttgttgt ttagttttgg gcataaaaat aagtaaaata aaactgccat gttaatcatc
      661 gcagttcgac cttttgagcc aaatatcatg tctcggcagg ttttacacct aggctacctg
      721 ctatctgcga cgattataat actagctaaa agacaatact atgtcaagta tttatttatt
      781 ggggtgagtt gctttatagt tatcaatctg tttttgaaga tcactatcta gctcaggaaa
      841 tttttctgga tattcagcta atatttcaat atatcctaac ttagctaatt gtctggcaac
      901 agctttgtta gattggggaa tttttccaaa actatttttt aagtcagttg ccagccattt
      961 tttggtatta acgtttttag ttttagctaa ttttgaaata attgctttat gatcggccgg
     1021 agtaaatgct tgtagattct ccgctacacg atagtcatag ccataattga ttatccccat
     1081 ggcaatatct ttatccagac cagacaattt gtaaagattg ttcgccagag cataaaattt
     1141 accttcttta ttgcctagat taatcagctg gtgggcaatt ttgttaaaat caacattaga
     1201 aaatttgtcc gcgtttttta gcaacgcatc actttgttta gccttaatta atttttgagc
     1261 aatttcctga tggtttagac cggaaaattt ttctaaatta tctgctacca catcaccttg
     1321 accactgtca attaattttt gagcaatttc ctgatggttt agaccggaaa atttttctaa
     1381 attctttgct accacatcac cttgatcctt gtcaattaat cgttgagcaa tttcctgatg
     1441 gtttagaccg gaaaattttt ctaaattatc tactaacgac cagccatagt gcttgtcaat
     1501 taatttttga gcaatttcct gatggtttag accggaaaat ttttctaaat tctttgctac
     1561 cacctgacca ttgttactgt caattaattg ttgagcaatc tttttatgga gtgagcgggg
     1621 aaatttgtca atatttttgc ctaattcatc tattagggag tcaccaccaa atatcaggga
     1681 cttcttatac tttataatcc cattagcaat agtttcataa tcttcatcag aaaaatttac
     1741 cttagaa
//
LOCUS       DAOOIS010000056         1698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_57344_length_1698_cov_39.454656, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000056 DAOOIS010000000
VERSION     DAOOIS010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57344_length_1698_cov_39.454656"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..979)
                     /locus_tag="PK960_02980"
     CDS             complement(38..979)
                     /locus_tag="PK960_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPD08158.1"
                     /translation="MTYCIDSVSFNLLKEVKMTYINNVGLTPRRFFNLAQVLAYNLRK
                     RYLELEKSGRALMGQMNQFGESSVIADWELEEAVIKFWQATGFKIGWITEEHGSFNLK
                     GAEYVAYLDGLDGSRVYVEGEGGRCGTMLGMYLAGKPDFSGYLGSLLVDYGQRETGGD
                     NTLIAAAGLGAWVDDTEEQGKYIKVQVDNSPKSHKKLIAYADLYFAPVRQAATHLPKV
                     KFIYLKASLPYYVDLVEGYSWLVLECTRKHSLEIAAAYPLVHEAGGVMVDVNSGKELG
                     KLDRSIANTHGRLIVSTTNLDIATKLRDELYPLTIKR"
     gene            complement(1468..1623)
                     /locus_tag="PK960_02985"
     CDS             complement(1468..1623)
                     /locus_tag="PK960_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08159.1"
                     /translation="MTLFRDIREELKENKETLPVDKVEAKKSLLILFLVLLGLGLVVT
                     VIWFITG"
BASE COUNT          552 a          409 c          269 g          468 t
ORIGIN      
        1 ctggggattg ttaaattact aattaaaaga taacacatta tcttttaatt gtcaaggggt
       61 aaagttcatc ccgtaacttg gtagcaatgt ctaaattggt agtagaaaca atgagccgac
      121 cgtgagtatt agctattgac cggtcaagtt tccctaactc tttgcctgaa ttaacatcca
      181 ccattactcc tccggcctca tgcactaaag gataagccgc ggctatttct aaagagtgct
      241 tacgggtaca ctctaaaacc aaccaactgt atccctcaac caaatcaaca taataaggca
      301 gagaagcttt aaggtaaata aattttacct tagggagatg agtagctgcc tgacgcaccg
      361 gagcaaaata aagatcggca taggcaataa gttttttgtg ggacttaggg ctattatcaa
      421 cctgaacctt tatatatttc ccctgctctt ccgtatcatc aacccaggcg cccagaccag
      481 ccgcagcaat aagggtgtta tctccaccag tctcacgttg gccataatct accaacaaac
      541 tgcccagata accagaaaaa tctggcttac cagctaaata cattccgagc atcgtgccac
      601 accgaccacc ctcaccttct acatataccc tagagccatc cagcccgtcc aaataggcca
      661 catactctgc ccccttcaga ttaaaagagc catgctcctc agtaatccag ccaatcttaa
      721 agcccgtggc ttgccaaaat ttaataacag cttcttccaa ttcccaatcg gcaattactg
      781 aggactcacc aaattgattc atttgaccca ttaaggctcg gccgctcttc tccaattcta
      841 aataacgctt tctaagatta taggccaaga cctgggccag gttaaaaaac cgacggggtg
      901 ttaatccaac attgttaatg taggtcattt ttacctcctt taataaatta aaactaacac
      961 tatctataca atatgtcaaa taaaaaagca ataaaaaccg ccatagaaac tatggcgggg
     1021 gaaaactagg gaactggaat ccaaagcatc accataataa caaaagccag agctatgatg
     1081 gccataacga gaacagcaat agtattgccg acctctttcc tgtccctatt agaaacttta
     1141 ggtaagccct tatgtcttgt tgtcatttta tcctcctaag ttttaaaagt gctattactt
     1201 aaactaaagg aaataaaaaa tttgtcaata gcagtaaaaa ccgccatatt tctatggcgg
     1261 cgttgaagtt tttattgggg agaattacca caacatcgca ataagcttta caaaaccaaa
     1321 aatcaatccc gttaacgtgg ccaataccac caaatcagaa atgatgctta taacaatgta
     1381 gtctctattc gccttccttt taatatcata aacctgaata cccccctgtt tctctttttt
     1441 ttcattttaa cctcctggtg ggcagaatca tccggtgata aaccaaatca ccgtgaccac
     1501 aaggccaagg cccaataaaa caagaaagag tattaagaga ctcttttttg cctctacctt
     1561 gtctactggc aatgtctcct tattctcttt taattcttcc cgaatatctc gaaataatgt
     1621 cattggttcc ccctcatttt taaattgtta agatactgcc ctaaaaatta aaactaatta
     1681 aaaaaattgt caataacc
//
LOCUS       DAOOIS010000057         1537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_66596_length_1537_cov_36.923077, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000057 DAOOIS010000000
VERSION     DAOOIS010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66596_length_1537_cov_36.923077"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /locus_tag="PK960_02990"
     CDS             complement(<1..1372)
                     /locus_tag="PK960_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HPD08160.1"
                     /translation="MLDNFTEKAQEAIINAQMVAQQYGQEFIGALHLLLALLQQDEGL
                     VRPIIEAQKIDPEYIIDQVEAELEKLPKSDHPQIADGIVQGTAEASMVIERARREAKN
                     LDDEYVATEHLLLAIVGVKSRAQAILLKAGLGYESLLKTLIELRGSQAVGSTDAEKKY
                     KVLQKYAINLTDLARQKKLDPVIGRDEEIRRIMQVLTRRTKNNPVLIGEAGTGKTAIV
                     EGLAQRIVSGDVPETLKGKELVSLDLGLLIAGAKFRGEFEDRFKSVIKEVQKSNGQVI
                     LFVDELHTIVGAGASEGSMDASNMLKPALARGEIKLIGATTIKEYQKYIEKDAALERR
                     FQPIFIEEPSVEDTIAILRGIKEKYEVHHGVRITDDALIAAAKLSARYITDRFLPDKA
                     VDLIDEAASSLRMEVDSSPDELDRLKREIRRLEIMKVGLLKEDNKDKVRKINRDLENL
                     KEKAKEL"
BASE COUNT          449 a          343 c          245 g          500 t
ORIGIN      
        1 ccaattcctt agctttttcc tttaagtttt ctaaatcgcg gttaatcttt ctgactttat
       61 ctttattatc ttctttcagt aatcccactt tcataatttc tagtcgtcga atttctcttt
      121 ttagcctatc tagctcgtca ggagaggagt caacttccat tcttaaacta gaggccgctt
      181 catcaatgag gtccacagct ttatctggta aaaaacggtc agtaatatac cgcgccgata
      241 atttggccgc ggcgatcaga gcatcatcag taatgcggac gccatgatgt acctcatatt
      301 tttctttaat accccttaaa atagcaatag tatcttctac actaggttct tcaataaaaa
      361 tgggctgaaa acgacgctct agggccgcat ctttttcaat atatttttgg tactctttaa
      421 tggtagtagc gccaattaat ttaatttcgc cccgtgctaa ggctggtttc aacatattag
      481 aggcatccat tgatccctct gaagcaccag cgccaacaat agtatgcagt tcgtcaacaa
      541 ataaaataac ttgaccattg cttttttgga cttctttaat aactgactta aaacgatctt
      601 caaattcacc ccgaaattta gctccagcaa ttaataagcc taaatctaaa cttactaatt
      661 ctttgccctt tagagtttcc gggacatcgc cactaacaat ccgttgggcc aaaccttcta
      721 caatagcagt cttaccagtg ccggcttcac caatcaacac tggattattt ttagtacgcc
      781 ttgtgagtac ctgcataatc cgccgaattt cttcatcacg gccaataact ggatctagct
      841 ttttttgcct ggctaaatca gttaaattaa tagcatattt ctgtaatact ttgtattttt
      901 tctcggcatc agtagagcca acggcttgag aaccgcgaag ttcaatcaga gttttgagta
      961 gggactcata gcccagtcca gccttcagta aaatagcctg agctctagat ttaaccccaa
     1021 caatagctaa taataaatgc tccgtagcca catattcgtc gtctaaattt ttagcttctc
     1081 ttctagctcg ctctataacc atggaagcct cagctgtacc ttgaactata ccatccgcta
     1141 tttgcggatg atcagattta ggtaattttt ccagctcagc ctctacctgg tcaataatat
     1201 attcaggatc aatcttttga gcctcaataa taggccgcac caacccttca tcttgctgca
     1261 gtaaagctaa caataaatga agcgcgccaa taaattcctg gccgtattgt tgggcaacca
     1321 tttgggcatt gataatagct tcttgagctt tttcagtaaa attatctaac atattttatt
     1381 attattattt tagcagtcta ttaattagag tgctaaaaat aatataactc agtttagtct
     1441 acctgtcaag attatagcac aaaataggca attaccaaaa tggttgacaa ataattgact
     1501 taggactagg gtaatatatc tataaaatgt acctaaa
//
LOCUS       DAOOIS010000058         1504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_68835_length_1504_cov_67.868185, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000058 DAOOIS010000000
VERSION     DAOOIS010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68835_length_1504_cov_67.868185"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PK960_02995"
     CDS             complement(<1..426)
                     /locus_tag="PK960_02995"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HPD08161.1"
                     /translation="MQKTLKVLIWIAAILAVIAVILSIWSVVQSKKINNQEAQVEQIV
                     NQVLSTDLANKPADENPVNPLIGTTWQIVSLTNSQDVLDLSSYDNSIQFSDQEISGRI
                     CNSFSGNYKIVDGIHIVADGPITRTEMACDNDLMDVENAF"
     gene            complement(446..718)
                     /locus_tag="PK960_03000"
     CDS             complement(446..718)
                     /locus_tag="PK960_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPD08162.1"
                     /translation="MYFNEALGALADPTRRKILKLLSRRNLSAGEIGRHFDITAPSLS
                     HHLSVLLMAGLVNQSRQGRSLIYSLKPGAIEMVANKILAIVRESNK"
     gene            839..1351
                     /locus_tag="PK960_03005"
     CDS             839..1351
                     /locus_tag="PK960_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08163.1"
                     /translation="MPYDQNQLWKPTRPYILGSGKYAGKALEYVLLQDLPAFLTMKYQ
                     LESNIKEDCKPNEYHRHLMWLVDGINLLASRVKCSECDQNALYIPARGNMAEGYYYLA
                     YPLCKKCVDSNYEWTRSTIFSITPWHFYTNIKKPEQKRIWKVIKIILKIDHMTSQQLF
                     QLLLSNYGPI"
     gene            1415..1504
                     /locus_tag="PK960_03010"
     tRNA            1415..1504
                     /locus_tag="PK960_03010"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1449..1451,aa:Ser,seq:tga)
BASE COUNT          489 a          290 c          259 g          466 t
ORIGIN      
        1 aaaagcattt tcaacatcca ttagatcatt atcacaggcc atttctgttc tagtaattgg
       61 tccatcagct acaatatgaa taccatcaac tattttataa tttccactaa aactattaca
      121 gatgcggcca gagatttcct ggtcactaaa ttgaatagaa ttatcataac tggataaatc
      181 taaaacatct tgactattag ttaaagaaac aatttgccac gttgtgccaa ttagtgggtt
      241 aactggattt tcatcagctg gcttattggc taagtcggta gacaatactt gattgacaat
      301 ttgctctacc tgcgcttcct gattattgat ttttttagat tgcactactg accaaataga
      361 taaaataaca gcaataacag ccaaaattgc tgctatccat attaatactt tgagagtttt
      421 ttgcataaga taaaattaat aatatttatt tatttgattc cctaacgatg gctaatattt
      481 tattagctac catttcaatc gcccctggtt ttaatgagta aattaaagac cgtccttgcc
      541 gtgactgatt tacaagtcca gccatgagca acactgataa atgatgagat aaagatggcg
      601 cagtgatatc aaaatgacgg cctatctccc cggccgataa atttcgccta cttaataatt
      661 taagaatttt ccgtcttgta ggatcagcta atgctcccaa tgcttcattg aaatacataa
      721 aaatatttag atgactgact aaattatatt ctaattattg tctcccgtca actattgaca
      781 aaaatggact gatatagtat acatattaga aatttgtacc ttaaaaagga ggtaaaaaat
      841 gccttacgac caaaaccaat tatggaaacc taccagaccc tatattttag ggtcaggtaa
      901 atatgccgga aaggccctgg agtatgtatt acttcaagac ctgcccgctt ttctgaccat
      961 gaaatatcag ctagagtcta acatcaaaga agactgtaaa cccaacgaat atcatcgcca
     1021 tcttatgtgg ctagtggatg gcatcaattt attagctagt agggtaaaat gttcggagtg
     1081 tgatcagaat gctttgtata tccctgccag agggaatatg gcggagggat attactatct
     1141 cgcatatccc ctctgcaaaa agtgtgttga tagcaattat gaatggacga gatcaacaat
     1201 cttctccata actccctggc atttttatac taatattaaa aagcctgaac aaaaaagaat
     1261 ctggaaggtc ataaaaataa ttttgaaaat tgatcatatg actagccagc agctcttcca
     1321 acttttactg tctaactatg gtcccattta atgggacctt ttatttaata gctattgcca
     1381 tagaccacta tttttgttag tctttagata atccggagag gtgccagagt ggttgaatgg
     1441 aacagtcttg aaaactgtca tccgtgcaag cggatcgcgg gttcgaatcc cgccctctcc
     1501 gcca
//
LOCUS       DAOOIS010000059         1482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_70419_length_1482_cov_48.337071, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000059 DAOOIS010000000
VERSION     DAOOIS010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70419_length_1482_cov_48.337071"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PK960_03015"
     CDS             complement(<1..253)
                     /locus_tag="PK960_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPD08164.1"
                     /translation="MPRLTATTDHHPGALPPTVTPTSSLTNPLWVSVSEAAKLGGVQT
                     KTIRRAISGGLITYRIRGNRYLIDFGSLLNFLRSRTKLNN"
     gene            733..1257
                     /locus_tag="PK960_03020"
     CDS             733..1257
                     /locus_tag="PK960_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPD08165.1"
                     /translation="MRAYEAYRAGIYRFIYFRVNSEAEAQDLTSSVFLKVWSVVQAGK
                     LKDYRSLRSLLYTVARHTVIDYYREGSGGNVSLDDETAEIEVVDNRLDIQALMEQDSD
                     LKLIYEALDQLKPEYREVIMLHFVSELSVKEIAEVIDKKPGNVRVILHRGLEALRKLT
                     KEEEEIKQEDTITI"
     gene            1254..>1482
                     /locus_tag="PK960_03025"
     CDS             1254..>1482
                     /locus_tag="PK960_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08166.1"
                     /translation="MNESGIIQKLKKLQSIQPNKDWEAKQHSVMEAQVYNGELITPRE
                     PSRFSNFLLLFKATFSQPGIMVGTILTIIIVG"
BASE COUNT          474 a          245 c          307 g          456 t
ORIGIN      
        1 tattatttag tttagtgcgg gaccttaaaa aattaagaag agagccaaaa tcaatgagat
       61 agcgattgcc acggatgcgg taggtaataa gaccaccgct aatagcccgg cggatagttt
      121 tagtttgcac tccacctagt ttagcagctt cagagactga cacccataaa ggattagtca
      181 gggaagaggt gggggtgact gtcgggggaa gagctccagg atggtggtca gtagtggcag
      241 ttaatcttgg catatagtct aagaattact agactttaaa ataaatgatt tttaattttt
      301 agtcaactag ggctttaagt ctaaaaatat ctagacctaa agacgacaat tagtttttta
      361 atgatttttt aagtctagta ttttctagac ttaaaatagc catctaccca attttttgtc
      421 taataaagtc taatttactc tggacaaatc attaggattc cctattgtaa actactatct
      481 aaaaaaaagc aagaccttaa ttttgcttat cttaattaaa tagggtaaac ttaaaacagt
      541 gccaaagtat tttttcttgt tttctccctt tatttatgtt agattatatc atccccttaa
      601 tggggtgtaa caattattca tttttttcgt ctatataaac gaaccgtaat gatgtcaacc
      661 ttaacttctc ataaacaacc cagcgaccgt ttgttatttt tacgcttaaa gaacaaggat
      721 caagccgcat ttatgcgcgc ttatgaggct tatcgcgccg ggatttatcg gtttatctat
      781 ttccgggtta attcggaggc cgaagcccaa gatttaacct cctctgtgtt tctgaaagta
      841 tggtcggtag tccaggctgg taaattaaaa gattaccgca gtttaagaag tttgctctat
      901 actgtggccc gtcatacggt tatagattat tatcgggaag ggtcaggagg taatgtttct
      961 ttagatgatg agacagcaga gattgaagtt gtggataacc ggctagatat acaggcttta
     1021 atggagcagg atagcgacct taaacttatt tatgaggcgc tagaccagtt aaagccagaa
     1081 tatcgggaag taattatgct ccattttgtc agtgaattgt cagttaaaga aattgctgaa
     1141 gtaattgata aaaaaccagg caatgttcga gttatcttac atcggggatt agaagcttta
     1201 cgtaaattaa ccaaagagga ggaagaaatt aaacaagaag atactattac tatatgaacg
     1261 aatcagggat tatccaaaaa ttaaaaaaat tacagagcat ccagccaaat aaagactggg
     1321 aggctaagca acacagcgta atggaagctc aggtatataa tggtgagtta ataacgccac
     1381 gggagcctag ccgttttagt aattttttat tattgtttaa agccactttt agtcaacccg
     1441 gcattatggt cggcactatt ttgaccatca ttatagtggg cg
//
LOCUS       DAOOIS010000060         1347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_81599_length_1347_cov_66.885449, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000060 DAOOIS010000000
VERSION     DAOOIS010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81599_length_1347_cov_66.885449"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..132)
                     /locus_tag="PK960_03030"
     tRNA            complement(57..132)
                     /locus_tag="PK960_03030"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(97..99),aa:Met,seq:cat)
     gene            complement(144..215)
                     /locus_tag="PK960_03035"
     tRNA            complement(144..215)
                     /locus_tag="PK960_03035"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(180..182),aa:Arg,seq:tcg)
     gene            complement(259..558)
                     /locus_tag="PK960_03040"
     CDS             complement(259..558)
                     /locus_tag="PK960_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012770737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPD08167.1"
                     /translation="MKENKINKADLIDKISETVNISKKQIEKVLDSLLDNITSGLKAG
                     KTINLVGFGSFMPKKRHARNGVNPQNPKQKIHIPEIIVAKFRTGKALKDALKNKR"
     gene            complement(563..784)
                     /gene="secG"
                     /locus_tag="PK960_03045"
     CDS             complement(563..784)
                     /gene="secG"
                     /locus_tag="PK960_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPD08168.1"
                     /translation="MRWLNIIQIIIALLLIGAILLQNRGADMSGLFGGGGDIYRTKRG
                     IEKTLFIATIVLAVIFFGLSLVGLIINKY"
     gene            complement(789..1133)
                     /gene="rplT"
                     /locus_tag="PK960_03050"
     CDS             complement(789..1133)
                     /gene="rplT"
                     /locus_tag="PK960_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPD08169.1"
                     /translation="MTRVKRGTIHTKKRHNILSKTKGFKWGRKNLIRLASTAATKAGA
                     HSYTDRRKKKRNARALWQIRIGAFAKDHNLSYSKMMNLLKKANIKLDRKSLADLAANH
                     PDVLSKILEQIQ"
     gene            complement(1146..>1347)
                     /gene="rpmI"
                     /locus_tag="PK960_03055"
     CDS             complement(1146..>1347)
                     /gene="rpmI"
                     /locus_tag="PK960_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPD08170.1"
                     /translation="NMPKLKTYQPVAKRMKLTKTGKIKRRKAGQDHFNAREDGQTGRM
                     KRRDFNVAKSDSKNIKKFLPYC"
BASE COUNT          365 a          252 c          255 g          475 t
ORIGIN      
        1 tttgcttaag aggcaactgc tctaccagct gagctatggg cgcattctat tgttgttggt
       61 ggcgggggtg gggattgaac ccacgacctt ggcgttatga gtgccatgct ctgccaactg
      121 agctacccag cctcgccatc tagcgctccc gtcaggattc gaacccaaaa tacttggtcc
      181 gaagccaagc gtgatatcca tttcaccacg ggagcttttc aaaaatagta tacattatat
      241 tgttgtaaaa gtgaagtctt atcgtttgtt tttaagagca tcttttaagg ctttcccggt
      301 tctaaattta gcaactataa tttctgggat atgaattttt tgtttaggat tttgaggatt
      361 aacgccattt cgcgcatgtc tttttttagg cataaaactg ccgaaaccaa ccaggttaat
      421 agttttaccg gcctttaatc cactagtgat gttatctagg agtgaatcta gaactttttc
      481 aatttgtttt ttgctaatat ttactgtctc cgagattttg tcaattaagt cagctttgtt
      541 gattttattt tccttcatat gtttaatatt tattaattat taaaccaact aatgataaac
      601 caaagaaaat tactgccaag acgatagtag ctataaataa agttttttca attcctcttt
      661 tcgtccgata aatatcacca ccaccgccaa atagaccgga catgtcagcg ccacgatttt
      721 gtaataatat agcgccaatt aacaggagag caatgataat ttgtataata ttaagccatc
      781 tcatggattt attgaatttg ttctaatatt ttagaaagga catccgggtg attagctgct
      841 aaatcggcca agcttttacg gtctaattta atattagctt tttttaaaag attcatcatc
      901 ttagaatagg acagattatg gtctttagca aaagcaccaa tccggatttg ccacaaggcg
      961 cgggcatttc tttttttctt gcgacggtca gtataagaat gagctccagc tttagtggcg
     1021 gccgttgaag ctagacggat taaattttta cgaccccatt taaatccttt ggtcttagat
     1081 aaaatattgt ggcgtttttt agtatggata gtgcctcttt ttactctagt catattaatt
     1141 tagttttaac agtagggcaa gaattttttg atgtttttgc tgtcagactt ggcaacatta
     1201 aagtcacgac gtttcattcg accagtttga ccatcttcgc gggcgttaaa atgatcttgt
     1261 cctgccttgc gtcgtttaat tttaccagtt ttagtaagtt tcatccgctt agcaactggc
     1321 tgataagtct ttaatttcgg catattt
//
LOCUS       DAOOIS010000061         1345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_81785_length_1345_cov_38.201550, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000061 DAOOIS010000000
VERSION     DAOOIS010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81785_length_1345_cov_38.201550"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PK960_03060"
     CDS             complement(<1..896)
                     /locus_tag="PK960_03060"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HPD08171.1"
                     /translation="MLSLDTPISVISGVGEAMVDKLAKLGIKTIGQAVYYYPFRYDDF
                     THRTTINRVVAGETVNVVGTIDVINTRRSPRQHMYVTEAIITDDTGKLRVTWFNQPFL
                     SRNLQTGDYISLAGKVSQDLLGPLMISPQYEKVDRRGITQHTSGLVPVYSTISGLTTK
                     QLRAIIGRTLNLAKIDEWLPSELLKKRGLLSLREALINIHRPPSWTSLEAARHRLAFD
                     ELVILQLRGQLIRERWQTSQAPIIRFNEALTKEIVNSWSFQLTDDQRRCSWQILQDLE
                     QNQPMLRLLQGDVGSGKTAVAI"
     gene            complement(962..1033)
                     /locus_tag="PK960_03065"
     tRNA            complement(962..1033)
                     /locus_tag="PK960_03065"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(998..1000),aa:Arg,seq:gcg)
     gene            complement(1041..>1345)
                     /locus_tag="PK960_03070"
     CDS             complement(1041..>1345)
                     /locus_tag="PK960_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08172.1"
                     /translation="FNENEEPEMLDFIIGTEGIMLGHKFYDYDEFKSFSVIYKPAQGI
                     KSLYLDFTSPWRRDLMIPLLDNNPLPIRENLLKYLKEDLERTGESLSETIGKLLKL"
BASE COUNT          427 a          297 c          215 g          406 t
ORIGIN      
        1 aaaatagcta ctgctgtctt cccgctccct acatcaccct gaagcaagcg cagcattggc
       61 tgattctgct ctaaatcttg aagaatctgc cagctgcaac gccgctggtc atcagttaat
      121 tgaaaagacc agctattaac aatttctttg gttaaagctt cattaaatct aataattggc
      181 gcttgactag tctgccatct ctccctaatt aactgacccc gtagctgcaa gataactaat
      241 tcatcaaaag ccagccgatg tcgtgctgcc tcgagactag tccaacttgg aggccgatga
      301 atatttatta aagcctctct taatgagaga agtccccgtt tttttaataa ttcggatggc
      361 agccactcat ctatcttggc taaatttaaa gttcgtccaa taatggctcg caactgttta
      421 gtagttaaac cactaatagt agaataaact ggcactaaac cacttgtatg ttgagttatg
      481 cctctcctat ctaccttttc atactgaggc gatatcatta acggccctag taaatcttga
      541 ctaaccttac cggccagtga tatatagtct ccggtttgta aattacgtga caaaaatggc
      601 tgattaaacc aagttaccct tagcttcccg gtatcatcag ttataatagc ttcggttaca
      661 tacatatgct gacgagggct acgtctcgta ttaataacat caatagtacc aactacattt
      721 accgtctctc cggcaacaac ccgattaatg gtcgtccgat gggtaaaatc atcataacga
      781 aaaggataat aataaactgc ctggccaatg gttttaatgc ccagtttagc tagcttatct
      841 accattgctt cgccaacacc tgaaataacg ctaattggtg tatcaagaga taacataata
      901 gtatattgta tcacaaatta acccctaaaa taaaaagagt ctcatgactc ttttataaaa
      961 gtactctcgg taggaatcga acctacatta caagatccgc aatcttgtgt cctatccatt
     1021 gaacgacgag agcttcacaa ttaaagtttt aaaagctttc caattgtctc gcttagtgat
     1081 tcaccggtac gctctaaatc ttctttcaga tattttaata aattttcacg aattggcaag
     1141 ggattattat ctaataaagg aatcattaga tcacggcgcc atggtgaagt aaaatctaga
     1201 tacaatgatt taataccttg ggctggttta taaataacag aaaaactttt aaattcatca
     1261 taatcataaa acttatgccc caacataatc ccctcagtac caataataaa atctaacatt
     1321 tctggctctt cgttttcatt aaaaa
//
LOCUS       DAOOIS010000062         1335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_82732_length_1335_cov_45.681250, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000062 DAOOIS010000000
VERSION     DAOOIS010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82732_length_1335_cov_45.681250"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="PK960_03075"
     CDS             complement(<1..958)
                     /locus_tag="PK960_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08173.1"
                     /translation="MKRSTYLIIFGLIICAGLISFINWQNNHRALPQVAVPSMSSSSE
                     PAIVTGPSLEDTKEVATALPVGQAKRQVTLPQDSEFIVSGNTASSGIRFIEGSIKPLD
                     VHVGDTQNFRIVVTSPNGIKRVVAEIETDKGINEVELTREGMISILDIHPNPYVVSPK
                     DNSLQILSAKELAQAREKEYEQELASKHGDKVNAAQGEKEVWTGSWVVRDTHNKNYIT
                     TFVAYDSAGNSEKMSLAWSDLCGIPLSGDWSTASTSFGCTIDTASTDGVENGNVEIIH
                     ERDLNISNGANFVWNDGKAITIRQGAKITINTGAKLVQAYLYV"
     gene            1091..>1335
                     /locus_tag="PK960_03080"
     CDS             1091..>1335
                     /locus_tag="PK960_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08174.1"
                     /translation="MKQIHYLFIAISLMAMTALLGAQEAPQIVDVDQLPFSVEMVSDA
                     VVMGNFCYIKTLWPFYIIKVDLLTKEVIWQQPHSANG"
BASE COUNT          422 a          287 c          242 g          384 t
ORIGIN      
        1 gtacatataa ataagcctgc accaactttg ccccagtgtt aatagttatc ttcgcgccct
       61 gtcgtatagt tatggccttc ccatcattcc aaacaaaatt agccccattg gaaatattaa
      121 gatctctctc atgtataatc tcaacattgc cattttccac cccatctgtg gaagcagtat
      181 caattgtaca gccaaaagaa gtagaagccg tgctccaatc gccacttaaa ggaattccac
      241 acaaatctga ccaagctaga gacatttttt cactattgcc ggcgctatca taggcgacaa
      301 aagtggtgat ataattttta ttatgggtat ctctaaccac ccaggaacca gtccagactt
      361 ctttttcgcc ttgcgcggca ttaactttat ctccatgttt actagctaat tcttgctcat
      421 attctttttc cctagcttga gctaactctt tagcggatag aatctgtaaa ctattgtctt
      481 tggggctaac aacataagga ttggggtgga tatccaaaat actaatcatc ccttcccggg
      541 ttaattctac ttcattaata cccttgtcgg tctcaatttc tgcgactacc cgtttaatcc
      601 cattaggaga agttaccaca atacgaaaat tttgcgtatc accgacatgg acatcgagcg
      661 gtttaatgga gccttcaata aagcggatac cagagctagc tgtattgccg ctgactataa
      721 attcactatc ctgcggcaga gtaacctgac gcttggcttg gcctacgggg agagcagtag
      781 cgacctcctt tgtgtcttct aaactgggcc cagtaactat ggctggttcg ctgctacttg
      841 acatagatgg tacagctact tgaggcaagg ctctatgatt attttgccag ttaataaaac
      901 taattaaccc agcacaaata ataaggccaa agataataag ataagtggaa cgtttcataa
      961 aaagtgaaaa aataattatg aagatattat atcagaaata agaagtaaaa gaaaccattg
     1021 acaaaaaata taaactatga cataataaaa ctataagtac tttaaaaata aaaatataag
     1081 gaggtggacc atgaaacaga tccactatct ttttatagcc atctcactaa tggctatgac
     1141 tgccctattg ggagctcaag aagcgccaca aatagtagat gttgaccaat taccctttag
     1201 tgtcgaaatg gtctccgatg ccgtagtgat gggcaatttt tgctatataa aaactctttg
     1261 gccattttac ataataaagg tggatctctt aactaaagag gtaatctggc aacaacccca
     1321 ttctgctaac gggga
//
LOCUS       DAOOIS010000063         1317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_84543_length_1317_cov_31.460380, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000063 DAOOIS010000000
VERSION     DAOOIS010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84543_length_1317_cov_31.460380"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PK960_03085"
     CDS             <1..284
                     /locus_tag="PK960_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08175.1"
                     /translation="FYNLASAQPTVSLSLLKNQGSLKEVNKSLERASSETGTGVVVYA
                     TGAIDLQSWLQDFSPVGSEHFVTFTSNLPLSSTMELILPILSQRLAEIQ"
     gene            296..368
                     /locus_tag="PK960_03090"
     tRNA            296..368
                     /locus_tag="PK960_03090"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:329..331,aa:Ile,seq:tat)
     gene            438..514
                     /locus_tag="PK960_03095"
     tRNA            438..514
                     /locus_tag="PK960_03095"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:472..474,aa:Val,seq:gac)
     gene            645..1142
                     /locus_tag="PK960_03100"
     CDS             645..1142
                     /locus_tag="PK960_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPD08176.1"
                     /translation="MEFDENDQIECSPNGIKHESAGGFVFFENDKTHELFVALLRKTD
                     GRYLIPKGHIRKGEKTQDAAIREVREELNLKEIPEIISFLRIDSYTFTLDDSNTTHYK
                     NVYLYVFRLNEKAEIKPKIDEGFETAEWIPFEEAVQKISFDKENLLRARQCFYYNKPS
                     RPIRT"
BASE COUNT          413 a          261 c          269 g          374 t
ORIGIN      
        1 ttttttataa cctggcctca gctcagccaa ctgtttctct gtcactgcta aagaatcagg
       61 gttctcttaa agaagttaat aagtcattag aacgtgctag ttcagaaact ggaacagggg
      121 tggtggttta tgccactgga gctattgatc ttcagtcatg gctacaagat tttagtccgg
      181 tggggtcaga acattttgtt actttcacta gtaatttacc tctttcgtcc accatggaat
      241 taattctgcc cattttaagc caacggttgg cagaaataca ataataaatt actaagcgcc
      301 cgtagctcag cggttagagc atcgctctta taaagcgggg gtcgatggtt caaatccatc
      361 cgggcgcacc taggcggggt ataaattttg ttatggttga caatgggagt ataaattctt
      421 acaataaacc ttaccaagga cggttagctc aggtggttag agcgctacgt tgacatcgta
      481 gaggtcactg gttcaagtcc agtatcgtcc accattgaat taatcacttc acttaggcag
      541 ttttgcgagc aaattaaggg gctttttgga atcaattttt tcgccctcgg tgaaagacgg
      601 agccgctttt ttaatctgag ttaaaaattg acataatatc aattatggaa tttgatgaaa
      661 atgaccaaat cgaatgttcg ccaaacggca taaaacatga atctgctggg ggttttgttt
      721 tctttgaaaa tgacaaaact cacgaattat tcgttgcctt attacgtaaa accgacggtc
      781 gttatctcat tccaaaaggc catattagaa agggtgaaaa aacacaggat gctgctataa
      841 gagaagttag agaggagctt aatttgaagg aaatcccaga aatcatttct tttctaagaa
      901 ttgacagcta tacctttact ctcgacgata gtaatactac ccattacaaa aatgtctacc
      961 tctatgtatt ccgacttaat gaaaaagcgg agatcaaacc taagatcgac gagggatttg
     1021 aaacggccga atggattcca tttgaagaag cggttcaaaa gatttccttt gataaggaaa
     1081 atctacttag ggcacgacag tgcttttatt acaacaagcc atcaagacct ataaggacat
     1141 gacggacata aaatctctaa ctattgccat ccctacctac aacggggaag ttacaatatc
     1201 caaaactctt aaattaagac atgcctttag ctttagagag ccaataggct agtccaccaa
     1261 ttaccccagc aattaataaa ccccctagcc aagtaaatgg cgagatagtc ttggtgg
//
LOCUS       DAOOIS010000064         1228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_94359_length_1228_cov_50.104859, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000064 DAOOIS010000000
VERSION     DAOOIS010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94359_length_1228_cov_50.104859"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PK960_03105"
     CDS             <1..549
                     /locus_tag="PK960_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HPD08177.1"
                     /translation="FDRDKLQERLAKLAGGVAVIKVGAATEVEMKEKKSRIEDALNAT
                     RAATMEGVVPGGGLALALASEAFELNDNSSVGRKIVAGAILEPIKQIATNAGQDGSFV
                     LYNIIDKNKDSNKPIGYNAITGKFEDMIEAGIIDPTKVVRCALENAASMAMMFLTTEA
                     IITEKKEEKTSGSNPNGMMEEM"
     gene            700..>1228
                     /locus_tag="PK960_03110"
     CDS             700..>1228
                     /locus_tag="PK960_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD08178.1"
                     /translation="MSQHKSHINFIITLLAIGWGLLVAVTALRAQWSGPNAQPPGGNP
                     SSFLTSGTSSETKTGPLTIDNNFYVTGNTSLGSTATPTSARVVITPVSNAAIDAGNGS
                     IRTTYLPSDDYDVVTKTYLASSLSGSSYWLLSTSTNSLYPTSTDWKVAIGTTTAGTGK
                     LVVMGGNVGIGTINPG"
BASE COUNT          339 a          216 c          285 g          388 t
ORIGIN      
        1 tttgaccggg ataaactgca agagcgttta gctaaattag caggtggagt ggcagttatt
       61 aaggtgggag cagcgactga agtagagatg aaggaaaaga agagccgtat tgaggatgct
      121 ctaaatgcta ctcgggcagc taccatggaa ggtgtggtgc cgggtggcgg cttagccttg
      181 gccctagcca gtgaagcttt tgagcttaac gacaatagca gtgtcgggcg taagattgtg
      241 gctggtgcta ttctagagcc aattaagcag attgctacta atgctggtca agatggctcc
      301 tttgtcctct ataatattat tgacaaaaat aaagatagta ataagcccat tggatataat
      361 gctataactg gtaagtttga agatatgatt gaagctggta ttattgaccc aaccaaagta
      421 gtgcgttgtg ctttagaaaa tgcggccagc atggccatga tgttcctcac gactgaagct
      481 attattactg aaaaaaagga agagaaaact tctggatcca atcctaatgg aatgatggag
      541 gagatgtaaa attattattt tttctctttt ttattcttaa atattttttc tattttaact
      601 caagataccc tggttaaacc agggtatctt ttgtccttct tgtatatttt agacaatttg
      661 ttgtataata actttattat ggttggctaa tttaagttaa tgtcacaaca caaaagccac
      721 attaatttta ttataaccct cctagcaata gggtggggtc ttttggttgc cgttactgcc
      781 ttgcgggccc agtggtctgg tcctaatgct cagccacccg gaggtaatcc ctcatccttt
      841 ttaacctctg gcactagttc ggaaactaaa acagggccac tgacgattga taataatttt
      901 tatgtcactg gtaataccag tttaggtagt actgctactc ctacctcggc tagggtggtt
      961 attactcccg ttagtaatgc cgccattgat gctggcaatg gttctatccg tactacatat
     1021 ttgccaagcg atgattatga tgtggttact aagacttatt tagcttcatc actaagtggt
     1081 agtagttatt ggttgttgtc tacttctact aatagccttt atcctactag tactgattgg
     1141 aaagtggcca taggtactac tacagctggt acaggcaagt tagttgttat gggtggtaat
     1201 gtgggaattg ggacgataaa tccaggag
//
LOCUS       DAOOIS010000065         1204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_97374_length_1204_cov_57.319408, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000065 DAOOIS010000000
VERSION     DAOOIS010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97374_length_1204_cov_57.319408"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="PK960_03115"
     CDS             complement(<1..1021)
                     /locus_tag="PK960_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPD08179.1"
                     /translation="MDHPKILIIKVDQLGDLLFSTFLLPIIKEAYPASSIDYLIHPHG
                     QAILDNNPAIRQVYYWQDLLLQNIPGRQKMKLGKRTRQFLATGYRNFKSWLALRHEHY
                     DIVINARAFWPSCNWPWRGLGHYLIAFDISQASYLANYLANYNLRAQEWENYLNLLKP
                     LHIKEEILKKYEPRGQFFNFVPAPQKPTVPYWCLSPVSFDPERTWSPSLWLSFIQIFL
                     DNFSDNSLVITGLANQQDWLAAITQPLNNNDRVITLTNLSLPQLADLLKDSQGLISLD
                     SFNVHLAIALNKPVFCLVNSRLFYVPGLSTNHLVDGRCMIPLLPNVVLNDLLTTTPEQ
                     LSSQIK"
BASE COUNT          409 a          220 c          223 g          352 t
ORIGIN      
        1 ttttaatttg actgcttaac tgctcgggag tagtggttaa tagatcatta agcaccacgt
       61 taggcaataa aggaatcata caacggccat caactagatg attagtactt aatcccggca
      121 cataaaaaag ccggctatta acaagacaaa aaactggctt atttagggct atcgctaaat
      181 ggacattaaa agagtcaaga cttattaacc cttgactatc ttttaataaa tcagctaact
      241 gcggtaggga taaattagtt aaagtaataa ccctgtcatt attgtttaaa ggctgagtta
      301 tggctgccag ccagtcctgt tgattagcta gaccggttat taccaaagaa ttatcagaaa
      361 aattatctag gaatatctga ataaaagaca gccataaact tggtgaccag gttctttcag
      421 gatcaaatga taccggcgat aaacaccaat aaggaacagt tggtttttgc ggggcaggaa
      481 caaaattaaa aaattgtccc cgtggctcat attttttaag aatttcttct ttaatgtgta
      541 atggctttaa caaattaaga tagttctccc actcttgcgc ccgtaaatta taattagcta
      601 aataattagc taaataactg gcctgactga tgtcaaaagc gataagataa tgacccagtc
      661 cccgccatgg ccaattacag cttggccaaa aagcgcgagc gttaataact atgtcataat
      721 gctcatggcg cagggctagc cagctcttaa aattacgata accagtcgct aagaattgcc
      781 gggtacgctt tcctaatttc attttttgcc ggccaggaat attttgtagc aataaatctt
      841 gccaataata tacctgtcta atcgctggat tattgtctaa aatagcctga ccgtgcggat
      901 gaattaagta atcaatagag cttgctggat aagcttcttt aataataggt aataaaaagg
      961 tagaaaataa taaatcacct aattggtcta ctttaataat taaaatcttg ggatgatcca
     1021 caatgtgttt aattggtcgg ggccagatta atatatcgcc cagcaattca aagggccagg
     1081 ttattagaaa ccagcagccc ttaaatagtt taataactaa ccgtctagga cttaccttta
     1141 gataagtata gttagcaata aatttgtgcc acaacttaat atggcgttta agtcttaaca
     1201 taac
//
LOCUS       DAOOIS010000066         1201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_97761_length_1201_cov_42.540140, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000066 DAOOIS010000000
VERSION     DAOOIS010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97761_length_1201_cov_42.540140"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..>1201
                     /locus_tag="PK960_03120"
     CDS             57..>1201
                     /locus_tag="PK960_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPD08180.1"
                     /translation="MALNNNLLARQKLAAALFPTIEESPQMIEARYPRRQLKLGAMVT
                     RIAPSPTGFMHLGGLYTALISERLAHQSGGIFFLRIEDTDSKRAVPGALELIINSLAH
                     FGLKLDEGETELGREIGDYGPYQQSKRASIYQAFVKQLIVSGQAYPAFETVEELEELT
                     KKQEASKVAPGYYGPWAYWRDKTPAEALAMLDKGFKPVIRWRSNGDPEHQVIVNDMFK
                     GRLALRESNQDIVLLKSDGLPTYHLAHVIDDHLMGTTHVIRGDEWLSSLPLHLQLFAT
                     LGWQPPIYGHLAPIQKLVGQSRRKLSKRHDPEASVTFYDEAGYPPEAVIAYLLNLANP
                     SFEAWFMSHPDKRWEDYLLIIAELKKGAGALLDFQKLDSYSKEIISRL"
BASE COUNT          332 a          233 c          276 g          360 t
ORIGIN      
        1 ttttttaggg ctgaaatata gtcagcagcc tatttttgtg ttattataat ggtattatgg
       61 cgctaaataa taatttgcta gcccgacaaa aattagctgc ggctttattt cctacaattg
      121 aggaatctcc ccagatgatt gaagcgcgtt atccgcgacg tcagcttaag cttggagcaa
      181 tggttactag aattgcccct agccctactg gctttatgca tttaggggga ctctatactg
      241 cccttatttc tgagcggtta gcccatcaat caggggggat ctttttcttg cgaattgaag
      301 atacagatag caagcgggca gtaccaggcg ccctagagct tattattaac tctcttgccc
      361 attttggtct gaagttagat gaaggcgaaa cagaactagg ccgggaaatc ggggactatg
      421 ggccatatca acaaagtaag cgagcctcta tttaccaggc atttgttaaa caattaatag
      481 tgagtggtca agcttatcca gcttttgaaa ccgtagaaga gttagaagaa cttactaaaa
      541 agcaggaagc ctctaaagta gctcctggtt attatggccc ttgggcttac tggcgagata
      601 aaacgccagc tgaggcacta gctatgttag ataaaggctt taagccggtt attagatggc
      661 gctctaatgg tgatcctgaa caccaggtca tagttaatga tatgtttaag ggccgtctag
      721 ccctacggga aagtaaccag gatattgtcc ttttaaagtc tgatggcctg ccaacttatc
      781 atttagccca tgttattgac gaccatttaa tgggcactac tcatgttatc cggggtgatg
      841 aatggttatc ttctttgccg cttcatcttc aattatttgc caccttaggt tggcaacccc
      901 caatatatgg tcatttagcg cctatccaaa aattagttgg tcaatcgcgc cgtaaattaa
      961 gtaaacggca tgatccagag gccagtgtaa ctttttatga tgaggcaggc tatccgcctg
     1021 aggcggtgat agcttatctg cttaatttag ccaatcccag ttttgaagct tggtttatga
     1081 gccatccgga caagaggtgg gaggattatt tactaattat tgctgagtta aaaaagggag
     1141 ctggagcttt gttagatttt caaaaattag atagttatag taaagaaatt attagccgtc
     1201 t
//
LOCUS       DAOOIS010000067         1031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2773
            NODE_125178_length_1031_cov_49.026639, whole genome shotgun
            sequence.
ACCESSION   DAOOIS010000067 DAOOIS010000000
VERSION     DAOOIS010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864848
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 625
            CDSs (total)                      :: 587
            Genes (coding)                    :: 584
            CDSs (with protein)               :: 584
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125178_length_1031_cov_49.026639"
                     /isolate="AMR_MDS_2773"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PK960_03125"
     CDS             <1..794
                     /locus_tag="PK960_03125"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPD08181.1"
                     /translation="SQKIVKVSQNKAKVPKKPINWRKLRRGVIIVGLVIIVLGLGYGL
                     FCSNYFRVRDIEISGTEFLSAPEIHDRALTALTGPVWQLWRGNFWLTTSRRVCRNLED
                     YQLIGCRVRRHWPNKLTLVVTEEPPVAIWQEGSRYYLVDRFGRIIKEDSAVNASSWPV
                     IQNQGDNLANNNKVAANPALWTMILAIKNSQWPNGEPHNFIFNNNEPNSLRVIGTQGQ
                     IIKLNLRHDLESQLALWRMGQSKFSTQLSEAKIIDLRYGDRIIYQ"
BASE COUNT          293 a          160 c          236 g          342 t
ORIGIN      
        1 cttctcaaaa aatagttaaa gttagccaaa ataaagctaa agtacctaaa aaacctataa
       61 attggcgaaa gcttaggcgg ggagttataa tagtagggct agtaattatt gttttgggac
      121 tgggctatgg ccttttctgc tctaattatt ttcgggtcag agatattgaa attagtggta
      181 ctgaattcct atcagctcca gagatacatg atcgggcgtt gactgcttta accggacccg
      241 tgtggcagct gtggcggggt aatttttggc tgacaacctc acggcgggta tgtcggaatt
      301 tagaggatta ccagctgata ggctgtcgtg tacgtcgcca ttggcctaat aaattaaccc
      361 tagtggtcac cgaagagccg cctgtggcta tatggcaaga aggtagtaga tattatttag
      421 tagatcggtt tggccggatt attaaagaag acagcgctgt taatgcttcc tcttggccag
      481 tgattcagaa tcagggagat aatttggcca ataacaataa agtagcagct aatccggcac
      541 tatggacaat gattttagct attaaaaatt cgcaatggcc gaatggtgag ccccataatt
      601 ttatttttaa taataatgag cctaattctt taagggtgat aggaactcaa ggtcagatta
      661 ttaaattaaa tctacgacat gatttagaat ctcagttagc cttgtggcga atggggcaga
      721 gtaaatttag cacgcaactt agtgaagcta aaattattga cttgaggtat ggagatagga
      781 taatctatca atagtttatt attttatttt atttgtatgg ataatgaggt aatttttaaa
      841 ttagttttct ttctggcagt attggttttt tgtgttttag tagttggaat ctttttagtg
      901 ctgtgtaaga ttttactgct ctttacccca gagctaaact ttatgggtct tattttgacc
      961 tatccctagg ggtaatgtcg tataagatgt attgtgcgac gttgtatatc attctccccg
     1021 ttaagacttg t
//
