LOCUS       DAOOJC010000001        26633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_1905_length_26633_cov_4.090940, whole genome shotgun sequence.
ACCESSION   DAOOJC010000001 DAOOJC010000000
VERSION     DAOOJC010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 26633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26633
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1905_length_26633_cov_4.090940"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..339)
                     /locus_tag="PLA01_00005"
     CDS             complement(85..339)
                     /locus_tag="PLA01_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99713.1"
                     /translation="MFESRKFIQKLSWTAVSHWAVGALGISVNLNDIPFSSPLVLKRN
                     DISYVYSTENLLTHWAVLTLIGVIFTVLTVLALNLNRKRE"
     gene            complement(332..2371)
                     /locus_tag="PLA01_00010"
     CDS             complement(332..2371)
                     /locus_tag="PLA01_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPC99714.1"
                     /translation="MQRHTITIYYKNSIDTVDLGTFNKNTVTMGRDPSNDIVIDSPII
                     SSHHALFQFVDDSWVLYDNNSKNGIMVNGKKEKMQRLNNNDILQIDNEKRPLKAAVVI
                     IYTVGAENAGEKWWEYAVHSNDFIYIGRDEQNSIVLNHNTISKKHAVFFHSDGKYYIE
                     DLKSTNGTYLNGTRITSMQPVKMNDVIYIGDTKIIFNGKSLLYNYYSKGFGIDAVSLR
                     RTVTVRNSSVFRKKKKTILSDVSVSINPGELVAIIGTSGAGKTTLLKCLSGLTKPDCG
                     TVMVNGDDFFKNYDVYMNILGYVPQEDAVYDNLTVEELLYYSINLRKSALSKTEIMNQ
                     IRQTIKDVELTGMEHTLIKNLSGGQKKRISIAMELLTEPKLLFLDEPTTGLDPQTEFN
                     IAMLLKKLSHDQKTVIFTTHSTSYLDVCDKIIVMGQEGRLCYFGTPKGTLDFFEVKDY
                     AHIYNLIHKNIEYWEDKYKKAHIRHKPLENNKASRHPRHFASIGTMSFQTIVLILRYL
                     KLNLRDKMRLAFLFMQVPLIAFLLGLITEGSKAFKIREEASQIILIVACAAVWVGFLN
                     SVQEISKERGIYNRERNAGLSSIAYIFSKIFVLGLFSLIQSFIFVEVLSWVIELPKPS
                     LLGSVKMELYATIFLTTLVSSVLGLCISSLVKNNDRALGITGSADLSDSFFRHLV"
     gene            complement(2548..2865)
                     /locus_tag="PLA01_00015"
     CDS             complement(2548..2865)
                     /locus_tag="PLA01_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99715.1"
                     /translation="MTDFSDCFNYCTMLAEIPEGLFANNTKVTSFMGCFNNCVNIKEI
                     PEGFFANNTEVRDYSKCFYGCSQLTGSAPNLWFKLNVIEYEDCFYGCTKLSNYEEIPD
                     DWK"
     gene            complement(2904..3446)
                     /locus_tag="PLA01_00020"
     CDS             complement(2904..3446)
                     /locus_tag="PLA01_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HPC99716.1"
                     /translation="MLLLIVTAVVFSFVSAGATLLYGDLNSDGLIDSTDLTMMKRVIL
                     RKLTADDITAADLDGDGELNSTDITLLKRYILKKITKFPVEELNPAITPTPTQEPTPT
                     PTETNEEVFAFKIKIDDKDLTYSFPIESSQTEYNIVVDWGDGIISEITDYSAREHEYG
                     EEGIYTIKVLSFDNMPHVPA"
     gene            complement(3764..4465)
                     /locus_tag="PLA01_00025"
     CDS             complement(3764..4465)
                     /locus_tag="PLA01_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99717.1"
                     /translation="MKGLKRIVIVTMLLALCTMALEGCRNEADSPGNELTGTPINSSK
                     GMQTGISAGTPGDSSQDASEEVIKEGKIEKKGNVILKQLAFVYQGNTIALSDIVDDKK
                     IESMLGKADEIKSHTYSVGDGKNMDPLNGLTGKQYYYPGLVIKTIDATEDKKSYIFSI
                     EITDPKYPTIRNIKVGDSFEKLKEVYPEGILLGGELTDEEDDYRYEPVNYVDVMNFHI
                     KEKKIESILIYTLLD"
     gene            complement(4568..5965)
                     /locus_tag="PLA01_00030"
     CDS             complement(4568..5965)
                     /locus_tag="PLA01_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPC99718.1"
                     /translation="MKYATKLLLTYLFFSVLSFSIIIICINKAIGYYGFVTIEKQMME
                     KADICELSFREILTRHEKSTAQVQPFDLAKDVLEVLKASGKEVRIYDNNLQLLGLAVN
                     GIIVNNGTPKIFEGNITNALNGNYSYSVTNNGLIYFAIPVQDMHYQNAYVFEIVEDIS
                     YFYSIMDKIRFILFLSAGGFTVLIALSSLYIARKTTKPIKHLLSATEKFSRQEFEQVD
                     LNRKDELGMLAEGLNQMGVKLNGYIQYQKQFVSNVSHELKTPLAAIRGFSQYLYEDKN
                     DDADLKKISFHLLNESNRLTKLIDELLILSKFDKGTQELNTEKEDLSELTDSVIQEMK
                     PEADKREISIESALQKGVFANVNKILMSHAIANILDNAIKYSKEGGHITVETSADQNT
                     AIIKITDQGIGINKEELPLVQERFYRSANSNIVKGSGLGLSLCREIVEKFNGRLIIES
                     EIGTGTTVSLLLPIA"
     gene            complement(5962..6660)
                     /locus_tag="PLA01_00035"
     CDS             complement(5962..6660)
                     /locus_tag="PLA01_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPC99719.1"
                     /translation="MKKILVIDDENAIRDLLEMVLVSENYQVRTAENGAAGLVEMDTF
                     EPDLVLLDIMLPDYSGYDLCREIVKKSAIPVIMISAKNETIDKVLGLELGAEDYITKP
                     FDNRELIARIKVVLRRYDHGDQNKGDIIQPIICGKLEINLESKQVLKDGNRISLTAKE
                     FKILETLSRKPKKIFTREELLEIVWGYDYLGDSRCVDMTIMRLRKKIEDDSENPKCVK
                     TIYGFGYQFGGDTD"
     gene            complement(6863..7447)
                     /locus_tag="PLA01_00040"
     CDS             complement(6863..7447)
                     /locus_tag="PLA01_00040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HPC99720.1"
                     /translation="MRQIKISAIVGSLRKESYNRQLALAAKEILKDRADFTLLEYQDI
                     PLMNQDIEFPAPEAVKRVREEVKSADGIWFFTPEYNHFFPGVLKNLIDWLSRPISNQE
                     PQVLGGKPAAISGISPGMSGTGIAQDHLVTLISYLNMEVMNTPRLTIPNAMQQLDSDG
                     RLALKESYPFLEMQANAFLNFVQKRMNDSLSRQA"
     gene            complement(7572..8138)
                     /locus_tag="PLA01_00045"
     CDS             complement(7572..8138)
                     /locus_tag="PLA01_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPC99721.1"
                     /translation="MRSFTTLDLQYAHRFYGFKGEAQYLHGHTGILTIEVEDTVNTGV
                     NMVFPCNEIKKIAWEVLQNFDHALILREDDPLLPAILGVYEAQGIKNGAPTNKQKGPA
                     FKTELATAYPECRLVVTKETMTVEGMIKIVYDLLKDKLNIVKLTFTSGVNGATEEYEP
                     QKNTLDRCPLCGIALNENGVCPKCGYKK"
     gene            8667..8939
                     /locus_tag="PLA01_00050"
     CDS             8667..8939
                     /locus_tag="PLA01_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99722.1"
                     /translation="MEKKIIKSTGISIDCTHTHANTIKKVPERILKHIAKKIFKSLEK
                     EAGAIPEGINTDIPNYKEIEDHKEAKDTMKTYVEEIIAQVEEKTEL"
     gene            9027..9773
                     /locus_tag="PLA01_00055"
     CDS             9027..9773
                     /locus_tag="PLA01_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPC99723.1"
                     /translation="MVDQDARVGYKSKTDNYFGYKTEYAITTGERIITAVRAYDGAYV
                     DGTGFKDLIEQTKDCGIKIKEVYGYKAYFRKPILDIIKENEAEAYIPVSEMVYKIDES
                     KYTYNKDSDQWFCDFGNYTVEKKKEIRRSDGREFWRYKFDKNGCQNCPNKDQCFKSGK
                     SNVLKMSVNTPEFYEYNQRAKTQEFLEKYKERSCQEWKNGEMKRFHGLSRARGYGLRS
                     MSLQSKLTALAVNLKRIAHLVSSLNFVSNN"
     gene            9974..11212
                     /locus_tag="PLA01_00060"
     CDS             9974..11212
                     /locus_tag="PLA01_00060"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPC99724.1"
                     /translation="MKKTFISLEQLRKIVKEYPTPFHLYDEKGIRENARRLKEAFSWN
                     KGFKEYFAVKATPNPTILKILKEEGCGVDCSSYTELLLSDKVGFHGDEIMFSSNVTPK
                     EDFVLAKKLNSLINLDDITHINFLEESAGLPETICCRYNPGGAFAVDNAIMDTPGEAK
                     YGFTREQLTEGFKKLMDKGVKHFGLHAFLASNTIANEYYPALAKLLFKTAVELKNETG
                     AHIAFINLSGGIGIPYRPDEKPADIYAVGDGVKKAYEEILVPAGMGDVSIYTELGRFM
                     LGPYGCLVTTVLHEKKIHKNYIGLDACAANLMRPAMYGSYHHITVMGKENEPHDHKYD
                     VTGGLCENNDKFAIDRMLPKTDIGDFIVIHDTGAHGFAMGYNYNGKLRSAEVLLKTDG
                     TTQLIRRAETPEDYFATLNI"
     gene            11401..12228
                     /locus_tag="PLA01_00065"
     CDS             11401..12228
                     /locus_tag="PLA01_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99725.1"
                     /translation="MKKVNINKSGGFIETLTAKWWFLALVPIIFFFSPPYVQRNGYPL
                     SDFSKWYATIGEIGSKNFTSYFAKYSIIMNLIAIAVIILAFVLKNKFSSLFSIYIAIM
                     MAFYGITQNTSYTEENGLGIVTSSYIVLPLLSGVWIWEAFVRKNDFSKPKKLNIWTIG
                     AFLLALFAFWNPIDSATLMPDFNPAYLLTNGSNSMFCTMTPMIIAVMFFIYPNINTAV
                     LRVTGITGATIGFIQILLHLVLMGKTNWWIGVIHIPVFLLSIIAVIVSFKADKIKNK"
     gene            complement(12329..18808)
                     /locus_tag="PLA01_00070"
     CDS             complement(12329..18808)
                     /locus_tag="PLA01_00070"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPC99726.1"
                     /translation="MFKRILSGFLVLAMVFAFLPESAFAASVLEHDTVIIQNEYIKVI
                     VSKKNGGYVISTLEGDILKKSDNNADLTHRGENFDTSFTSFRVDGEDIVFGTSTGTLS
                     TTNTDISVTSTWSKGGMIYQQTITLVNNAQSEQLGTAMITYVVKNNSSKAAQVKSRLL
                     IDTQLGDKDYGYYEIPRQNLGQGYEYFEFERTWEGGSMPADYFVRDNPFASSIVGYGV
                     NSVFEDMKPYKMTFAHWANIASTVFDYTPDSSLNFTNPINDEKTGDSAAALYYDLGSI
                     AAGGEKSFSTYYGVTANLKNKNNEILINSTAPAMLTLNEDKSAFIGTSGEADGIVRIN
                     TTIANPLSSEKTYKNIAIVVYANGFTPQRQNDGGDWISYDNTTPIYSEVVNFGPGENR
                     TTYFDFKFTPKESAELGSFVTKVFDMDPVVNNLGYYAEDYCLGTTENFIVIPGTDPTL
                     PAITLNDLQPKILYNNDIRYMTVVGRGVNFFNATQLNKIELRGDNGITYEIPIDNLTV
                     EQTKDGSAPTNASILLDKYMLPGRYQLHFLWKSDAGEEALQGVAEDFTSAAMTVFMTS
                     DEAFRNDKYGVVAIIRESGDNIYGIDSYKDETAFENAGIDEDDLLLTLRGPILKETKN
                     GKTTYRIAGNNSDVNINNVLNYHGGSLTIEDTGGTVTLLMDGKLTSIGANTTVRDGTA
                     MFKLEKGTDYVVPTYDSDGAIVDGDSLSNGQDFIELKWNSAVDTLQTIGGFLIDLRYG
                     ILGKIQDADDPGKTYNVISFGGGLDLSFMTPGGAAAARQNKSKHGGWTQSTKVSWEWD
                     TASAKYKPTGTGDVAPDLEDETTISGGVNVYDVLYGGKDPGYLGINMNAHIDMPQIVA
                     FLPNKIEGNIDINTIGLYQVGVDADVETSIFEMHLSFVIKESPSGAPIPDKLYFAIGG
                     FEPGVNIDGMGVLWVTGGGGGFDNLYETIYGYDGVPPLKLLLHVDFDVAKILTGNADL
                     ELSLRSIDIRFSDLSLKMLEDAKFLEEGGISLAWYPNFSLTADAQINFMEFFKGQFQI
                     SAYSNADVAFFFEFMMRIALSLPSDIPIVGGMEIAALELGGGTEKMWGSIELLGLLQI
                     GFTYWWSGEFEFSSGELQKSQTFAASSETMGLMRTQALFAGLSQPQEVQHNTKTGETQ
                     YMSVGSNLHFVTGSVIVGDVEAKINEANKPRLMRSFAAAAAAPSISTNTEGTVHLVQF
                     TDTGDYIITISRADGTEITAEYLKNHITVSHGSEPYTLKFYGESSGNGVYDNTTANVN
                     VANGIAYVIIPGEDLNSTKNFVLRFDDGKKYDAGAIYVEPLSSLTSTTASVSGQTLTV
                     GWTGENISDTAVINVTLSDEKKGSGIIVASGIAAKGKTSENITLPPTLAAGKYYVTLS
                     LSDEGKSFDAYTVDTPVTVTNINAPAAPKSVTLANAGNDKMKVTITPDDNDNKLEGYF
                     VDVYEDGKLIDASLYWAKDDIDNIVIGGRYDVPYVNEKGEPVVVDGKSYETLGYNPGS
                     KYSVRIRAGNVKAEAAGDVYYCSSFVTSNEVVLKAATPPSVTISYNDGAVTLRSNPKV
                     TGTLYVNIDTVYEISNADSYTQEINLPDGEHTFEFRGVDGDGDSVVVKEVISIDTTAP
                     VIMLEAPLGGVFSGDRIDLRGRITDADTEVEVGLKINGEAFDLPKNSFAEGVLDGSVM
                     LDSSENQSNVTVEIVATDRAGNSATKIVGLTNPQAANVEEIAVYFDGQPLIDGKFSLT
                     KIGDSAKLKVMGHLPDGSWIDVTGNDSVSLRLVGGNSVSLTDNTITAVAEGQTLLTAA
                     FDLGGGEMLQSGAVVLVGDVILYDALNNAIDKAQALSKDNYTAKSWAVLEEALTSGKL
                     ILTTDGVNQDIVNNAATTIINAIAGLEEKTPSNPSDPGQSGGSGSGGDNAVIAYTITF
                     NTNADNDIDSVEVVPGGKLAKPADPIKAKHTFTGWFLDKQLTMPYDFDKPVKGSFTLY
                     AGWYENEITEDITPKPDAWQNPFTDVSESDWFYSDVEFVYKKGLFHGTSKTTFSPKEP
                     MTRAMLVTVLWQQSGAPTVSGNTFTDVASDAYYYNAVNWAVQNGITYGISNDLFGPDM
                     EITREQMAAFLWRYAKFTNVDVSVGEDTNILSFEDAFEISEYAISAMQWACGEGIIVG
                     RPGGILDPKGGATRAEVAAMLHRFLEK"
     gene            complement(18838..26388)
                     /locus_tag="PLA01_00075"
     CDS             complement(18838..26388)
                     /locus_tag="PLA01_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99727.1"
                     /translation="MIKKNTWGKKPLSLLLAAVLILTLLPATVLAEDSNSYGHPVTYE
                     PLTSLKYKGKTVMFFDYSLSQFPAASGSKSSMVDQFTKLIQSGYSFNSFGERISASNT
                     AKAPETFNGEISGLKNYNASYKFDVPSANQYEYYFGGNIRGWNKKTGFITFYNQWEKA
                     SFSFMGFSHSTENDDYYHGTKNSGYDISKNSNWKSSMKMSDLKWRSNDNDFDNNNRAK
                     LGMGMVLGRDTEGPMVKSIIMTDSGGNAIPGNVITLDWLSKHPDRTIYFKLTLSEPVK
                     FENDVDPKDVALNIRTLGMDGTEGLLAKASFLHYAPEETGNSSMSETEMIFEYQVPDP
                     YTDNSSVTQERGLLYSFTRVNINASDNPVLFNKMRDISGNVASTSKEGEPTPYSVAMN
                     GKVDILPLSIAFGEKTAGIDIYRESETDTGKEFINQSELIKIQLTFNKPLALKYTDYT
                     KLPSITLNIKDKEGEYITITPTAYDDFLKRWYKSSITQEWTQSSSQSYAGTWRIINPA
                     DPYSYIQAPNALISSDGKSITYYYQAVPGHTFLEEDRIEVIEVNPNAGTKDASGYELL
                     KYKRTGNMLVPANAPSIIKESEQRDAKNLMKGVVPAHRYKLDFKEPSVDVDAALEGGV
                     AVFTANVDDANLEGCYASFNISFNGKIDGTLGYKPSNSLEYDDNNWSQTGTMVSADAP
                     IVEGKAYMLVKLPQAAEFDAATVTVTVADEAGNSGTKTKTLPIGYDTLSPRITLTQQY
                     DIDAPKPYNNYAAVDVLDISGTTVTYAWTDEEDSEPDNASYSETGTEIRYTANDLNEN
                     KIYEKTLWVKAADKKGNIGRSQMNFSFNNTCSDVNVITANNTDLLTKDVYPEATIKIQ
                     NAKAYWYAWAETTGAWTATWGALSGSTAADYVKAGLADNASGIYYRREGNIIAFSGEL
                     TDEDPEGEGYQNEEEISITAGESTKVLKHLKMGSLTEGGTGFQGTGDGISLNAADMLD
                     ISDTTRPLELLVAYLDSDDVWHVESIPFDTFYNTPEISNDPIRFSTNNRFGVREDTYR
                     LDLEKRYESYINYFENDSQYFNRPGLGWADESRSYNMPNIYGFAEVEFALGYDNVTGL
                     DRVKDIEVSLVKVKKPIYDLPYDVETVSTWSIDKNSLTPMGDMLYYGTNEGEESRMEH
                     IGNISKNLYSYTVSFDPGTIDKIAYEIDEYGMEYLISYEFRTKYTYVNNASETFTTAK
                     FVFDNNGWEIGRYESIQYNLLQRDSSPKLLQFENTQYDSPYTKFGALAVFNGDELVSN
                     VPVVPFNENSTINLYIKAQNVYTLLHELTARQRIAADYLDTEGRGLLGTGLVMRVGTL
                     VSEGKILNPTILNSGEESSDGSFEFVSEGNLPVGSLDEEGEAIVYYQLVDIARNIEGS
                     INAVKLKKDNTPPVVELSASVDNSMPASEVYVRITSVTDESDVEMMYDTPYAVQFGTP
                     QNVEELYYSDYNPGFFLTPDEDGGYTFTGNGFIVAIASDRADNKNETLIVNGTETPKY
                     EDDINRLDTYAVYHITNIDREPPEFTSEPLFTANADGSFDISAKVSSDVSRAYIKFDK
                     AYTELLTGETYEKDTDIPAFEFDNVMGKFDGKFNKTTHEINGKIYIMSDEKIKMTQAT
                     LIIEDEAGNSAISEWKPNGGLAGIEPKITNLIDPDKKVPVYKYAENLSFTVPVKLTEF
                     NTSLNTAHSSLPIYSDGVVIITYTDIFNRYFTQKVYADIFGGSHTFTVSPTVPTNGAV
                     TIEIIPSKGFTVSTYKTKMTENGNVTYTITPADGGSAQSYSIPITNIDKTAPQANVSV
                     AFDSTEDAAGNVYLYSAVYSLTGFSEEDVTVIGASSVSFDAMSDTRTHTFTFYDAAGN
                     VGTFDVDASDLTFVNPDDTKIAAYRLTYLSGELKIGEYTLGQEEPLTLTPSNTDISVK
                     VEALNAAGSVVPSKLSEQPVFTCESGTVSDEQSKTVTITGVGNDNSINATVLLPDDAI
                     DKMAPVGSVDYRPQTTGSVRVYLVHSNNDLADDGIKLIGQDKNGIPFELLSDGDGYYV
                     EFTSNGTGYFLLRDKAGNTSTIALAVASIDNDPPELGSEGWSSLINATSRDLDFAQKL
                     QQIITTPTNNSIKLFLSFNEQIYKADFDVYSVKANDVGSTAVKLTNKGDYALVTVSGS
                     SVVVEFLQNCQLRLTVYDIRNNSYTVWRPEDGPIIAIDKGAPKITNTPSIVYKDNKAS
                     ITYEFDEEVTLTQNAAASYQSKHNLTFDANGTFILTFADKAGNVVSTYPVVDKIDDIA
                     PVIIPELEYAGSGQDFGMQGTAGFYTSKDAKFTVRLEDSTTDGLALLVKRLSGFAVTV
                     TDGEFTITENGVYTITATDKWGQANSVNVSIDAIDKTAPSIRLASTEAVQAVPGTSPD
                     EVKSSLLEGVTATDLQSGVAPGFPSVSLEGVDLSKPGAYTATITVTDNCGNTATALRS
                     IAVADKAGKYLIINGSRISVGDIFVTSDKELVVDTAALGSEAFRPYYAKGYKTSAQMK
                     YATAFTDSFTISEKGYYTIVAQGENRERYVFYIYIY"
     gene            complement(26404..>26633)
                     /locus_tag="PLA01_00080"
     CDS             complement(26404..>26633)
                     /locus_tag="PLA01_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99728.1"
                     /translation="ISLHKHPHAGAFIVLSRIPRSEMSKKRWGLDARGDFLLGHGTKK
                     KKAKGHHIRALLYITCAYFTSRLHNTSIYNK"
BASE COUNT         7430 a         5891 c         5100 g         8212 t
ORIGIN      
        1 cttcttgttt ttgcatacag atattatatc aaatccctga ataatggcta tataattttt
       61 acataaaata tatttttact catttcattc cctttttcga ttcaagttta gagctaaaac
      121 ggttaatacg gtgaaaatca ccccgatcaa tgtcaatacc gcccaatggg tcaatagatt
      181 ctctgtactg tatacatatg aaatatcgtt tctttttaac acaagaggag aactgaaggg
      241 tatgtcattc aaattaaccg atattcccaa tgctcctaca gcccagtgac ttacagcagt
      301 ccatgaaagt ttttgaatga attttcggga ttcaaacaag atgcctgaaa aaagaatctg
      361 ataaatcagc actaccggta atgcctaggg cacggtcatt atttttcacc aatgatgaaa
      421 tgcacagtcc caaaacagat gacaccaaag tcgtcagaaa tatcgtcgca tacaattcca
      481 tcttaacgga tcctagaagt gaaggttttg ggagctctat aacccaagat aaaacttcaa
      541 caaaaataaa gctttgtatc aatgaaaaaa gacccagtac aaatattttc gaaaaaatat
      601 aggctatgct gctaaggccg gcattcctct ctctattata aataccccgt tccttggata
      661 tttcctgaac ggaatttaaa aatccaaccc atacagcagc acaagcgaca atcaatatta
      721 tttgactggc ctcttcgcgg attttaaagg ctttgctgcc ttcggttatc aggcctaaaa
      781 gaaatgcaat tagcgggacc tgcataaaaa gaaaagccaa tcgcattttg tccctgaggt
      841 taagcttcaa ataccttaaa atcagcacaa tcgtctgaaa tgacattgtt ccgatgcttg
      901 caaaatgccg tggatggcgt gaagccttat tattctcaag gggtttgtga cgaatatgag
      961 cttttttata cttgtcctcc caatactcaa tgtttttgtg tatcaggttg taaatatggg
     1021 cataatcctt tacttcaaaa aaatccagcg ttcctttagg tgtcccgaaa tagcacagtc
     1081 tgccctcctg ccccataact atgattttat cgcacacatc aagatatgaa gtggagtgcg
     1141 ttgtaaaaat aaccgttttt tggtcatggg acaatttctt tagaagcatg gcaatattaa
     1201 attcggtttg agggtcaagc cctgttgtgg gttcatccaa aaaaagcaat ttgggttcgg
     1261 tcaaaagttc catcgctata ctgatgcgtt ttttctgccc gccgctcaag ttctttatta
     1321 aagtatgctc catccctgtc agctcaacat cttttattgt ctgtcgaatt tgattcatta
     1381 tttcagtttt gctcagtgcc gattttcgaa gatttatcga ataataaagc agctcttcaa
     1441 cggtcagatt atcatatacc gcatcttcct gaggcacata tcccaagata ttcatgtaaa
     1501 catcatagtt tttaaaaaaa tcatcgccgt ttaccataac agtaccgcaa tcgggtttgg
     1561 ttaatccgct aaggcatttg agaagcgtag tctttcccgc accgctggta cctattatgg
     1621 caacaagctc tccgggattt atgcttacgc tgacatcact gagtattgtc tttttctttt
     1681 ttcgaaagac agaactgttc ctaacggtta cagtccttct taaagaaacg gcatctattc
     1741 cgaatccctt cgaataataa ttatatagca aggatttacc gttaaaaata atcttcgtat
     1801 ccccaatgta aatcacatca ttcattttca ccggttgcat tgatgtgatt cttgtaccgt
     1861 ttaaataagt accgtttgtg cttttcaagt cctcgatata atattttccg tcggaatgaa
     1921 aaaatacggc atgttttttg gatatagtat tatgattcaa tacaatactg ttttgttcat
     1981 ctctaccaat atatatgaag tcgttagaat ggacagcata ctcccaccat ttctcaccgg
     2041 cattctcagc tcccacagtg tatatgatga caacagcagc tttcaacggt cttttttcat
     2101 tgtcaatctg caggatatcg ttattattca agcgttgcat tttttccttt ttgccgttaa
     2161 ccattattcc gtttttgctg ttattatcgt ataataccca agagtcatcc acaaattgaa
     2221 ataatgcatg atgagaagag attatgggcg aatcaatgac aatatcgttt gatggatctc
     2281 tccccatcgt tacggtattt ttattaaaag tgccaaggtc aactgtatca atagaattct
     2341 tataatatat tgtaattgtg tgcctctgca tatcgttatg tacccttcgc atgtttacat
     2401 attttattcc aatgatatta taaatcagca aaaacgatta atcaaatata ttagtgttgt
     2461 ataatatttt gaatcttata tctcattgca tatctattca tttgcattgg caacaggctt
     2521 gcaccgacac ataaaacagg gaaaaattca cttccaatcg tccggtatct cttcgtagtt
     2581 ggataattta gtacagccat aaaaacaatc ttcatattct attacattta atttgaacca
     2641 taaattcggt gctgaaccgg ttaattggct gcagccgtaa aaacacttgc tgtaatccct
     2701 tacttcagta ttattagcaa aaaatccttc agggatctct tttatgttga cgcagttatt
     2761 gaaacagccc ataaagctgg ttactttagt attatttgca aaaagtcctt ccggaatctc
     2821 tgctaacatg gtgcaataat taaaacagtc actgaagtca gtcacattag tattattggc
     2881 aaacagccct tcgggaattt ctgttaagcc ggtacatgcg gcatattatc aaaggaaagt
     2941 acttttattg tatatatacc ttcttctcca tactcatgtt ctcttgcaga ataatctgtt
     3001 atctcgctta ttatgccatc gccccagtcg acaacaatat tatattctgt ttgtgaagat
     3061 tcgatgggaa agctatatgt gagatcttta tcatcaattt ttatcttgaa tgcaaaaact
     3121 tcttcatttg tttctgtagg agttggtgta ggttcctgcg taggggtggg tgttattgcc
     3181 gggttcaatt cttccaccgg aaactttgtt attttcttaa gtatgtacct ctttagcagt
     3241 gttatatccg ttgaattcaa ttcaccgtca ccgtcaagat cagcagctgt tatgtcgtcg
     3301 gcagtcaatt ttctgagtat taccctcttc atcatagtta aatcagttga gtctattaag
     3361 ccatctgaat ttaagtcacc atataataaa gtagccccgg cagaaacaaa gctgaagaca
     3421 actgcagtaa caatcagcag cagcaaaaat gttttttcat cataaaactc cctccccttt
     3481 atattaaata cttaattaat gcacataaag aacaacacaa gagatttcca attcattcta
     3541 tcgctagtga ttaacaacag tattaacaat ttaattatat tttacttgaa aattattgta
     3601 aagtttatta tctcattttg ccgcaatgaa ttattaaacc gtaaatggga aaaatcggca
     3661 taagatcaag gggacggttc ttccgtgtcg tatgacacgg aagaaccgtc ccctcgtgcg
     3721 ggagaaccgt ccccttgtgc tactcttgtt tgatgtggcg ggattaatcc aaaagcgtat
     3781 atattaaaat gctctcgatc tttttctctt ttatatgaaa attcattacg tcaacgtaat
     3841 taaccggttc ataccgataa tcatcctcct cgtcagtcaa ttctcctccc agcaggattc
     3901 cttcaggata aacctctttg agtttttcga agctgtcgcc aacttttata ttccttatag
     3961 ttgggtattt cgggtcagta atctcaatgc tgaaaatata cgactttttg tcttcagtag
     4021 catcaatagt ttttataaca agccccggat aataatattg ttttccagtc aatccgttaa
     4081 ggggatccat attctttcca tccccaacag aataggtatg agacttgatt tcatctgctt
     4141 ttcccagcat actctcgatt tttttatcat ccacaatatc ggaaagggca attgtattcc
     4201 cttgatagac aaaggccagc tgttttaata taacattccc cttcttttct attttgccct
     4261 cctttattac ttcttctgag gcatcctggg aactgtcccc aggggtaccg gcggatatgc
     4321 cggtctgcat tccttttgag gaatttatcg gtgtaccggt taattcattc cccggtgagt
     4381 cagcttcatt cctgcaccct tccagagcca ttgtacaaag tgctaaaagc attgtcacta
     4441 taacgattct ctttaaacct ttcattgccg ctcctccttc aaataatctg aaatgtttat
     4501 gttacagtgt tcattatata taaaaactat gttagggtaa taacaaactg gtatcaatct
     4561 tgtaacatta tgctataggg agcagaaggg aaacggtagt tccggttcca atctcacttt
     4621 ctatgattaa ccggccgttg aatttttcaa caatctctct gcaaagagaa agccccagcc
     4681 cggaaccttt gacaatatta gaattggctg acctataaaa cctttcctgc accaaaggaa
     4741 gctcctcctt attaataccg ataccttgat ccgtaatctt tatgattgct gtattttgat
     4801 cggcagaagt ctctactgtg atatgacctc cttctttcga gtactttatt gcattgtcta
     4861 aaatatttgc aattgcatgg gacatcaata ttttattaac attagcaaag actccttttt
     4921 gtaaggctga ttctatcgat atttctcttt tatctgcctc cggtttcatt tcctgaataa
     4981 cactgtcagt cagctccgat aaatcttcct tttctgtgtt aagctcctga gttcccttat
     5041 cgaattttga aagaattaaa agttcatcaa taagctttgt aaggcggttt gattcattta
     5101 gcagatgaaa ggatatcttt tttaaatcag catcgtcatt tttatcttca tataagtatt
     5161 gagaaaaccc gcgaattgct gccaacggag ttttcagctc atgagacaca ttagagacaa
     5221 actgcttttg atactgaata tagccgttta gtttaacacc catctgattt aatccttctg
     5281 ccagcatccc cagctcatct ttcctgttta agtcaacctg ttcaaattcc tgccttgaga
     5341 acttttccgt cgcagaaaga agatgtttta tgggctttgt tgttttacgg gcaatgtaca
     5401 ggcttgacag ggctatcaaa acagtaaacc cgcctgcact aagaaataat ataaatctga
     5461 tcttatccat aatactgtaa aagtaagata tatcttcgac tatctcgaaa acataagcat
     5521 tttggtaatg catatcctga acaggaattg caaaataaat aagaccgtta ttggttacag
     5581 agtaagaata atttccattc aaggcattag tgatattgcc ttcgaaaatc tttggagtgc
     5641 cgttattaac tattattcca tttacagcca accctaaaag ctgcaaatta ttatcataga
     5701 ttctgacttc cttacctgaa gccttcaata cttccagaac atcctttgcc aaatcgaaag
     5761 gttgtacttg agcagttgat ttttcatgcc ttgtgagtat ttctctgaaa gacagctcac
     5821 aaatatccgc cttttccatc atctgctttt ctattgtcac aaagccgtag taaccgattg
     5881 ctttatttat acagataata ataattgaaa aggataatac agaaaagaat aaataagtta
     5941 agagaagctt ggttgcgtac ttcaatcagt gtcaccacca aattgataac cgaaaccgta
     6001 tatggttttt acacatttcg gattctcaga atcatcttct attttctttc tcaatcgcat
     6061 aattgtcata tcaacgcacc gactgtctcc taaataatcg tacccccata caatttccag
     6121 aagttcttct ctggtaaaaa tctttttagg ttttcttgaa agtgtctcaa gaattttaaa
     6181 ttcttttgcc gtcagtgaaa ttctattgcc atccttcaat acctgttttg attctaaatt
     6241 gatttcaagc ttcccgcata ttattggctg tatgatatcg cctttatttt ggtcaccatg
     6301 atcatatctt cttaaaacaa ctttgattct ggctatgagt tctctgttat caaagggttt
     6361 tgtaatataa tcttcagctc caagctcaag ccctaagact ttatcgatag tttcattctt
     6421 tgcagatatc attataaccg gaattgcgga tttttttact atttccctgc aaaggtcata
     6481 gccggaatag tcgggcagca ttatgtccag caaaactaaa tccggctcaa atgtgtccat
     6541 ctccacaagt ccggcagcac cattttcagc agttctcacc tgatagtttt ccgatacaag
     6601 caccatttct aacaggtctc tgattgcgtt ttcatcgtca attacaagaa ttttcttcac
     6661 ctggaacttc tcccttaaat tagttttaac gaaacagacc ggcaccaaaa caaaaattca
     6721 tacagctaat tgattgacaa atctttatga tgaaattcac tgtgatgctc tatattatca
     6781 aacttatttt tgtatatata attatacatt aaagcaattg ccatttctat acctaaatta
     6841 tatattcaac caattgccat atctatgcct gcctgctaag ggagtcgttc attcgctttt
     6901 ggacgaaatt caaaaaagca ttcgcctgca tttccaggaa aggatagctt tctttaagtg
     6961 ccagcctgcc atcactgtca agttgttgca tagcattggg tatagtaagc cgaggagtat
     7021 tcataacttc catgttcaaa taactgataa gcgtaactaa atgatcctgg gctataccgg
     7081 ttccggacat accgggagaa attccgctta tagctgcagg ctttccgcca agaacctgag
     7141 gttcctgatt gctgatgggc cgggacaacc agtcaataag gtttttcagt acgccaggaa
     7201 aaaaatgatt gtattcgggt gtaaaaaacc aaataccgtc tgcagatttt acttcctcac
     7261 gaacccgttt tacagcctca ggtgcaggaa attcaatgtc ctggttcatt aacggtatgt
     7321 cctgatattc gagcaaagtg aagtctgccc ggtctttaag tatttctttt gctgcaagag
     7381 ccaactgccg gttataggat tcttttcgca gagaaccaac aatagctgat atcttgatct
     7441 gtctcattat gattcactct ccgtttcttg tctgatatta taagaaggca ctctaaacac
     7501 atttggtgta gagtgccttg atttgcttca accctgatac atttaaactg tatttaaaat
     7561 ctcaaattgg attatttctt gtatccgcat ttagggcaca caccgttttc atttaacgca
     7621 ataccgcata acgggcaacg gtccaaagta ttcttttgag gctcatattc ttccgtagct
     7681 ccgtttactc cgcttgtgaa tgtgagcttt actatattga gtttatcctt cagtaaatca
     7741 taaactattt taatcatgcc ttcaacagtc attgtttcct tcgtgacaac caaacggcat
     7801 tccggatagg ctgttgcaag ttcagtcttg aaagccggac ctttttgctt attggttggt
     7861 gcaccgttct taataccctg tgcttcgtaa accccgagaa tagcaggaag taatggatcg
     7921 tcttccctca aaataagtgc gtggtcaaag ttttgcaata cttcccacgc aattttctta
     7981 atctcattgc atgggaatac catattaaca cctgtgttga cagtgtcttc aacttcaatg
     8041 gtcagtattc cggtatgccc gtgcaaatac tgtgcttcac ctttgaaccc ataaaacctg
     8101 tgtgcatatt gcagatctaa cgtagtgaaa cttctcataa ccattctcct tcgtactata
     8161 tatttacggg attttcatag atttgctccc gtatgagcac acattccacc tgatttcttc
     8221 ctttagatga ggaaacgtcc tttttatata atttaggatg ccctccattc gctctttttt
     8281 attcagatcg ccaatattgt ccagaaaccc ttgaaatcca ttcatgttgt ccaccctttt
     8341 agaatcttcg cctaagataa tcctatcttt actactttac cactttaaaa gtgtgtctaa
     8401 acataaaaaa agtagaacat atagaacagg gaacggttcg aaaccactga aaaacactaa
     8461 ataaaaaatg ctaaatattc aacaaaattc tataaatata tagtaagaaa acccccttaa
     8521 tggtataatg gaattgacaa aaaaaaccaa caccaaaggg ggttttacaa tgcttaaaga
     8581 taaaaataat caattaagca tctgctcaat attatataat aaaattccag aaaatcatat
     8641 acttaaatta ataaataaag cagtgtgtgg aaaagaaaat aatcaagtca accggtatca
     8701 gtatcgattg tacacacact catgccaata ctataaagaa agtgcctgaa aggatactaa
     8761 agcatattgc aaagaagata tttaaaagtc ttgaaaaaga ggcaggagca attccggaag
     8821 gcattaatac agacattccc aactataaag aaatagaaga tcacaaggaa gcaaaagata
     8881 caatgaagac ttatgtggaa gaaattattg cacaagttga agaaaaaact gaactttaaa
     8941 aatatccaga gactaatgct attatccaaa atacccgtga tatattagca tatccaaaat
     9001 ttatacaaca aaaaggtata agatctctgg tagaccaaga tgcaagggta ggatataaat
     9061 caaaaacaga taactacttt ggatataaaa cagagtatgc aataacaaca ggagagagaa
     9121 taataacagc agtaagggca tatgatggag catatgttga cggtacaggc tttaaagatt
     9181 taattgaaca gacaaaagat tgtggaataa aaataaaaga ggtatatgga tataaagctt
     9241 atttcaggaa accaatttta gatataatta aagaaaatga agctgaagca tatatacctg
     9301 taagtgaaat ggtatacaaa attgatgaga gtaaatatac gtataataag gattccgacc
     9361 aatggttttg tgattttggg aattataccg tagagaaaaa gaaagagata agaagaagtg
     9421 acggaagaga attctggaga tataaatttg ataagaatgg atgtcagaac tgtcctaata
     9481 aagatcaatg ctttaagagt ggaaaatcga atgtacttaa aatgagtgtg aatacacctg
     9541 aattttatga atataaccaa agagctaaaa cgcaggagtt tctagaaaaa tataaagaac
     9601 gatcatgtca ggaatggaaa aacggagaga tgaagagatt tcatggatta agtcgtgccc
     9661 gggggtacgg cttacgaagc atgtcattgc agtcaaaatt aacagcatta gcagtaaatt
     9721 taaagaggat agcccacttg gtatcctctt taaattttgt tagcaataat taaaactaaa
     9781 tgtttttcgc tgtataaaac tgtaaaagta gcttgaaaaa aacgtacttt atcagtggtt
     9841 tcggacggtt ctctgttcga tgtttgatta ctaatcccag gtatattata atagtaaata
     9901 ttcattgaat agataatttt cgatgtcaat ttcattaaat atttacaata tgtcgaaagg
     9961 agtgagtttg aatatgaaaa agacattcat aagtttagaa cagttaagga aaattgtaaa
    10021 agaatatccg actccttttc acctctatga tgaaaaagga ataagggaaa atgcaagaag
    10081 attaaaagaa gctttctctt ggaacaaagg ctttaaagaa tattttgcgg ttaaggcaac
    10141 accaaacccg acaatattaa agattttaaa agaggaaggc tgcggtgtgg attgttcttc
    10201 ctatactgag cttttgctgt cggataaagt cggatttcat ggcgatgaaa taatgttttc
    10261 ctcgaatgta accccgaaag aggactttgt tcttgcgaaa aaattgaatt ccctgataaa
    10321 ccttgacgat attacccata ttaatttttt agaagaatct gcgggtcttc cggaaactat
    10381 ttgctgccgt tataatcccg gtggtgcttt tgcggttgac aatgccataa tggacactcc
    10441 cggcgaagct aaatacgggt ttacgcggga acagctgact gaaggcttta aaaagcttat
    10501 ggataaagga gtcaagcact ttggactgca tgcttttctg gcaagcaaca ccattgccaa
    10561 tgagtattat cccgcactgg caaagcttct ctttaagaca gctgttgaat taaagaatga
    10621 gaccggtgca catattgcat ttattaacct ttccggcggt atcggtatac cttaccgtcc
    10681 tgacgagaag ccagcggata tttatgctgt cggcgatgga gttaaaaaag cctatgaaga
    10741 aatccttgtt cctgcaggaa tgggcgatgt ttctatatat accgagcttg gaaggtttat
    10801 gttgggtccc tacggttgcc tggttacaac tgtgcttcac gaaaagaaaa tacataaaaa
    10861 ttacattggt ctggatgcat gtgctgcaaa tcttatgcgt cctgcaatgt acggttcata
    10921 ccatcacatt acagttatgg gaaaagaaaa tgagccccat gaccacaagt atgatgttac
    10981 cggcggctta tgcgaaaaca acgataaatt tgcaattgac cgcatgctgc ccaaaactga
    11041 tatcggtgac tttattgtaa tacatgacac cggtgcccac ggatttgcaa tgggttacaa
    11101 ctacaacgga aagctccgtt ctgcagaggt cttgttaaaa accgatggga caacccagct
    11161 tatacgccgt gcggaaaccc ccgaagacta ctttgcaacg ttgaatattt aagcaaaatc
    11221 aaggggatag caagggggga cggttcttcc gtgtcattcg acacggaaga accgtccccc
    11281 cttgctgttt tggtatccaa ctctgtctgc aaaaaaatct gataataaat aataattcgt
    11341 tgattagcac cgtatcttga tataaaatgt aagtaaaata tatattgtga ggtgtaatat
    11401 atgaaaaaag tgaatattaa caaaagtggt ggttttattg agaccctaac agccaaatgg
    11461 tggtttttag cacttgtccc tataatattc ttcttttccc ctccatacgt acaaagaaac
    11521 ggctatcctc taagtgattt ctcaaaatgg tatgcaacaa tcggagaaat aggatctaaa
    11581 aactttacga gctattttgc taaatattct ataataatga atttaatagc catagcagtt
    11641 attattcttg catttgttct aaaaaataaa ttcagctctc tattttcgat atatatagcg
    11701 ataatgatgg ctttttatgg cattacccaa aacacatcct ataccgaaga aaacggcctt
    11761 ggaatagtta caagttcata tattgtgtta cccttacttt caggagtatg gatatgggaa
    11821 gccttcgttc gcaaaaacga ttttagcaaa cccaaaaaat tgaacatatg gacaataggt
    11881 gcttttctac ttgctctatt tgctttttgg aatcctatag attctgcaac tctaatgcct
    11941 gattttaatc cggcctatct tctgacaaat ggatccaaca gtatgttttg cacaatgacc
    12001 ccaatgataa ttgctgtgat gttttttata taccccaata taaatacagc tgtactaagg
    12061 gttacaggga taacgggagc gacaatcggt tttatacaaa ttttattaca cttggtgtta
    12121 atgggtaaaa ccaactggtg gattggtgta atacatattc cggtgttttt gctttccata
    12181 attgctgtaa tagtttcatt caaggcagat aagattaaaa ataaataact tttcatcaag
    12241 acaagggggg acggttcttc cgtgtcgaat gacacggaag aaccgtcccc ttgtgccctt
    12301 gtgttcacct accggaaatg caaaacactt acttctccaa gaacctatgg agcatagccg
    12361 caacttcggc tcttgttgca ccgcccttcg ggtcgagaat tcctcctggc cttcctacga
    12421 ttatgccctc gccacaggcc cactgcatgg cacttatagc atattcggaa atttcgaatg
    12481 catcttcaaa agacagaata ttcgtatcct ctccaacaga tacatctaca ttcgtaaatt
    12541 ttgcataacg ccacaggaat gccgccatct gctcacgggt tatttccatg tcgggtccga
    12601 ataaatcatt actgattcca taagtaatac cgttttgcac cgcccagttt actgcattat
    12661 agtagtatgc gtccgaagct acatctgtaa atgtattgcc gcttaccgtt ggggcaccgc
    12721 tttgctgcca cagcactgtc acaagcattg cacgggtcat aggctccttc gggctgaatg
    12781 tggttttact tgtgccgtgg aataaaccct ttttataaac aaactccaca tcactataga
    12841 accaatcact ttccgaaaca tcagtaaacg gattttgcca tgcgtcaggt ttcggagtga
    12901 tatcttccgt tatttcattc tcataccaac cggcatatag tgtaaagctg cccttcacgg
    12961 gtttgtcaaa atcataaggc atggtaagct gtttatccaa aaaccatccg gtaaaggtgt
    13021 gtttcgcctt tatggggtca gcaggttttg ccagttttcc gccaggaaca acctctacgc
    13081 tgtctatatc attatctgca ttagtgttga atgttatcgt gtaggctatt acggcattat
    13141 ctccaccgct gccgcttcct ccactctgtc ccgggtctga aggatttgag ggcgtttttt
    13201 cttccagccc tgcaatggca tttataattg tagttgctgc gttgttgacg atatcctgat
    13261 tcactccgtc cgttgtaagg ataagcttgc cgcttgtcaa tgcttcctct aagacagccc
    13321 atgattttgc agtataatta tctttgctta gtgcttgtgc tttgtcaatt gcattattca
    13381 aagcatcata cagtatcaca tcgcctacaa gcaccactgc cccgctctgc agcatctctc
    13441 cgcctccaag gtcgaaagcc gccgtcaaca gtgtctgccc ttccgctacc gcggtaatcg
    13501 tattatcggt aagggaaacc gaattgccgc cgactaatct caggctgacg gaatcattgc
    13561 ccgtcacatc aatccaactg ccatcgggaa gatgtcccat tacctttaat tttgcactat
    13621 cgcctatttt ggtcaagctg aatttaccgt ctattagcgg ttgaccgtcg aagtagactg
    13681 caatttcctc aacatttgca gcttgaggat tcgtaagtcc cacaatcttg gttgcagaat
    13741 ttcctgccct gtctgtggca acaatctcaa ccgtaacatt tgactggttt tcgctgctgt
    13801 cgagcatcac gctgccatca agtactcctt ctgcaaaaga atttttgggt aagtcgaaag
    13861 cttcgccatt tatttttagt cccacttcta cctctgtgtc tgcatcggta atcctgcctc
    13921 taagatcaat cctgtcgccc gagaacacac cgccaagagg tgcctcaagc ataattacag
    13981 gtgcagtggt atcaatcgaa ataacctctt ttacaaccac actgtcgccg tccccgtcaa
    14041 ctcctctgaa ctcaaaggta tgctcaccgt caggaaggtt tatttcttgt gtataggaat
    14101 cggcatttga tatttcataa accgtatcaa tattcacata cagtgtaccg gttaccttgg
    14161 gatttgacct tagggtaact gcaccgtcat tgtagcttat cgttacgcta gggggagtag
    14221 ccgctttcag cacaacttcg tttgaagtca caaacgaact gcagtaatag acatcgcccg
    14281 ctgcttccgc tttcacatta ccggcacgaa tccgtaccga atatttgctg ccgggattgt
    14341 agccgagggt ttcatatgac tttccgtcca ctacaacagg ctcgcctttt tcattgacat
    14401 aaggcacatc ataccgcccg ccgataacga tattgtcaat atcgtctttt gcccagtaca
    14461 gactggcatc gataagctta ccgtcttcat aaacgtcaac aaaatagcct tcaagcttat
    14521 tgtcattatc gtccggagta attgtcacct tcatcttgtc gttccctgca tttgcaaggg
    14581 ttaccgactt gggtgcggcc ggtgcattga tatttgtaac ggtaacaggc gtatcaacag
    14641 tgtaagcatc aaagcttttt ccctcatccg acagagaaag ggtaacatag tattttcctg
    14701 ctgccagtgt tggcggtagt gttatgttct ctgatgtttt tccctttgct gcaattccgc
    14761 ttgcaacaat gattcccgaa cctttctttt cgtctgaaag ggttacatta attaccgctg
    14821 tatcactgat attttccccg gtccagccta ccgtcaaagt ttgaccgctg acacttgcag
    14881 tagttgatgt caaactgcta agcggctcaa cataaattgc accggcatca tattttttac
    14941 cgtcgtcaaa acggagtaca aaattctttg tgctgtttaa atcctccccg ggaataatca
    15001 catatgctat accgttggca acattcacat ttgcggtggt attatcataa acaccgttac
    15061 cgctggattc cccatagaat ttaagtgtgt aaggctccga accgtgagac acagtaatat
    15121 ggtttttcag atattccgct gttatttcgg taccgtcggc acggcttatt gttatgatat
    15181 agtctcccgt atcggtaaac tgtaccaagt gcacagttcc ctcggtattg gttgaaattg
    15241 atggagctgc agctgcagcg gcaaaggaac gcatcaagcg gggtttgttc gcttcattaa
    15301 tcttcgcctc aacatcgccc acaataacac tgcccgttac aaagtgcagg ttactgccca
    15361 cactcatata ctgggtttct cccgtttttg tgttatgctg aacctcctga ggctgagata
    15421 acccggcaaa cagggcttga gtccgcatga gtcccatagt ttccgaactg gcggcaaagg
    15481 tttgactctt ctgaagctcg ccgctggaaa actcaaattc cccgctccac cagtacgtaa
    15541 agccaatttg aagcaacccc agaagctcaa tcgaacccca catcttttct gtgccgccgc
    15601 caagttctaa agcagcaatc tccataccgc ccacgatggg gatatccgac ggaagggaca
    15661 gagcaattcg catcatgaat tcaaagaaga aggccacatc cgcattggaa tatgcactga
    15721 tttggaattg ccccttgaaa aactccatga agtttatctg tgcatccgcc gtaagggaga
    15781 aattcggata ccacgcaaga gaaatcccgc cctcttcaag gaattttgca tcctcaagca
    15841 ttttcaagct taaatcgctg aaccttatgt cgatggaacg aagtgataac tctaaatctg
    15901 cattaccggt aaggattttt gcaacgtcaa aatcaacatg taaaagcagt ttcaaaggcg
    15961 gtacgccatc atatccgtaa atggtttcgt agaggttgtc aaatcctccg ccgccaccgg
    16021 ttacccacag aacgcccata ccgtcaatgt ttacccccgg ctcaaatccg ccgatggcaa
    16081 aatacagttt gtcgggaatg ggagcaccgc ttgggctttc ctttataaca aatgaaagat
    16141 gcatttcaaa gatggacgtt tcaacgtcag catcaacacc gacctgatat agtccgattg
    16201 tgttgatgtc tatattgccc tcaattttat tcggcaaaaa ggcgacaatc tgcggcatgt
    16261 caatatgtgc attcatgttg attcccaaat acccggggtc cttgcctcca tacagcacat
    16321 cgtagacatt aactccaccg cttatagtgg tctcatcctc taaatccggt gctacgtcac
    16381 cggtgcctgt gggcttatac ttggccgaag ccgtatccca ttcccaactg actttggtgg
    16441 attgagtcca gccgccgtgt ttactcttgt tctggcgtgc tgctgccgca ccgcccggag
    16501 tcataaacga caggtcaagc ccaccgccaa aggaaatgac attgtaggtt ttgcccggat
    16561 cgtctgcatc ttgaattttt ccaagtatgc cataacgcag gtcaattaaa aatccaccga
    16621 tggtctgcaa ggtgtccaca gcactgttcc atttcaattc aataaagtcc tgaccgtttg
    16681 aaagggaatc accatccacg atcgcaccgt cggaatcata ggtgggaaca acatagtctg
    16741 tgcccttttc aagcttgaac attgccgtgc cgtcacggac agttgtattt gcaccgattg
    16801 aggtaagctt cccgtccata agaagggtca ccgtaccgcc ggtatcctct attgtaagac
    16861 ttccgccatg atagttcagc acattgttta tgtttacatc actgttgttt cctgcaattc
    16921 ggtatgttgt ttttccattc ttggtttcct ttaaaatcgg tccccttaag gtcagcaata
    16981 aatcatcttc gtcaataccg gcattttcaa aggcagtttc atccttgtat gaatctatgc
    17041 cgtaaatatt gtcaccggat tcgcgtatga ttgcaacaac accgtatttg tcgttgcgaa
    17101 aggcttcgtc acttgtcata aacacggtca tagccgccga agtaaaatcc tcggctaccc
    17161 cctgcaaagc ctcttcccct gcatcacttt tccacaaaaa gtgcagctgg taccggcccg
    17221 gaagcatata cttgtcaagc agaattgatg catttgtagg tgcactgccg tcttttgtct
    17281 gctcaacagt cagattatca atgggtattt catatgtaat cccgttgtca ccgcgaagtt
    17341 caattttatt cagctgtgtc gcattgaaaa agttcactcc ccggccaact accgtcatat
    17401 aacgaatatc gttgttatac agaatcttcg gctgaaggtc atttaaggtt atggctggca
    17461 aggtggggtc tgtgccggga atgacaatga agttttccgt tgtacccaga cagtaatcct
    17521 ctgcataata acccagatta ttcaccaccg gatccatatc aaacaccttt gtaacaaagc
    17581 ttcccagctc cgcactctct tttggtgtga acttgaaatc aaagtacgtg gtacggtttt
    17641 ctcccggtcc aaagtttacc acctctgagt agatgggggt tgtgttatcg taagaaatcc
    17701 aatccccacc gtcattttgc cgctgagggg taaaaccgtt ggcatacacc acgattgcga
    17761 tgtttttata tgttttttcg ctcgaaagcg ggtttgctat ggttgtattt atgcgaacaa
    17821 taccgtccgc ttcgccgctt gttcctataa atgccgattt atcttcgtta agggtaagca
    17881 ttgccggtgc agttgagttt ataaggattt cattattttt attttttaga tttgctgtaa
    17941 cgccgtagta ggttgagaaa gatttctcgc cgcccgccgc aatacttcca aggtcatagt
    18001 acagtgctgc agcactgtct cccgttttct catcgttgat ggggtttgta aagttaaggg
    18061 atgaatcggg cgtataatca aacaccgtag atgcaatatt ggcccaatgt gcaaaggtca
    18121 tcttataggg cttcatatcc tcaaacacac tgttaacgcc atagcctact atgcttgacg
    18181 cgaacgggtt gtctcgtaca aaataatcgg caggcatgga gccgccctcc caggtacgct
    18241 caaactcaaa atattcatag ccctgaccga gattctgacg gggaatctca tagtaaccgt
    18301 agtccttgtc accaagctgt gtgtcaatga ggagacggct tttaacctgt gcagcctttg
    18361 atgaattgtt ctttacaaca taggtaatca ttgccgttcc aagctgctcg gattgggcgt
    18421 tattgaccag cgtaatagtc tgctgatata tcatcccgcc ctttgaccat gtgcttgtaa
    18481 cactaatgtc ggtgttcgtt gtagacagag tacctgtgct tgtaccgaac acaatatcct
    18541 caccgtcaac acggaaggat gtaaaggatg tatcgaagtt ttcgccccgg tgcgttaaat
    18601 ccgcattgtt gtccgatttc ttcaatatgt ctccttcaag ggttgaaatc acatagcctc
    18661 cgtttttttt gctgacaatt acctttatat attcattttg tattataacc gtatcatgct
    18721 ccaaaacaga ggctgcaaat gcagactccg gtaaaaatgc aaacaccatt gcaagcacca
    18781 gaaaacccga taatattcgt ttaaacattt ctatgtacct cccttaatat actctcttta
    18841 ataaatgtag atgtagaaca catagcgttc gcggttttca ccctgtgcca caattgtata
    18901 atagcccttt tcacttatcg taaacgagtc ggtaaaggct gtcgcgtact tcatttgagc
    18961 agaagtttta tagcccttgg cataataagg cctaaacgcc tcactgccta acgccgctgt
    19021 gtcaacaaca agctccttgt cggaagtaac gaatatgtcg cccaccgaaa tcctgctgcc
    19081 gtttataatg aggtacttgc ccgctttgtc ggcaactgca atgcttcgta acgccgttgc
    19141 tgtattaccg caattatcgg tcaccgtaat ggtcgccgta tatgcacccg gtttggataa
    19201 gtctacgccc tccaaactta cagatgggaa gcctggtgca acccccgatt gaaggtccgt
    19261 tgccgtaaca ccctctaata aagagctttt aacctcatcc ggacttgtac ccggcacagc
    19321 ctgcactgcc tcggtagacg ccaggcgaat actcggtgca gtcttgtcta tggcatcaat
    19381 actgacattt acactgtttg cttgacccca tttgtccgtt gctgtgatgg tataaacgcc
    19441 attttccgtt atggtaaact caccgtcggt tactgtaacc gcaaatccgc tcaaacgctt
    19501 aaccaaaagt gcaagaccgt cggttgtgga atcttcaagc cgaacagtaa atttcgcatc
    19561 tttacttgtg taaaaaccgg ctgtcccctg catgccaaag tcttggccgg aaccagcata
    19621 ttcaagctcc ggtatgatta ccggtgcaat gtcatcaatt ttatcaacca caggataggt
    19681 gcttaccaca ttgcccgctt tgtcggcaaa ggtcagaata aatgtaccgt tcgcatcaaa
    19741 ggtaagattg tgcttggact ggtagctagc cgctgcattt tgcgtaagcg ttacctcctc
    19801 gtcgaattca taggttatgg atgccttgtt gtctttataa acgattgaag gtgtgtttgt
    19861 aattttaggt gcacctttgt caatcgcaat tatcggaccg tcctccggtc tccaaacggt
    19921 ataggaattg tttcgaatat cgtataccgt cagccttagc tggcaatttt gcaggaattc
    19981 aaccacaaca ctgctgcccg aaaccgtcac cagtgcataa tcgcctttgt tagtgagctt
    20041 cacagcagtt gaaccgacat catttgcctt tactgagtat acgtcaaaat ctgctttata
    20101 aatctgttca ttaaaggata aaaacagctt tattgagttg tttgtcggtg ttgttataat
    20161 ttgctgaagc ttttgggcga aatccaaatc ccttgaggtg gcatttataa gactgctcca
    20221 gccctcactg ccaagttcag gcggatcatt gtcaatgctt gctacggcaa gagcgattgt
    20281 tgaagtgttt cccgccttgt cacgaagtaa aaagtaacct gtgccgttgg aggtaaactc
    20341 tacatagtag ccgtcaccat ccgaaagcag ttcaaaggga attccatttt tatcctgccc
    20401 aattaacttt ataccgtcat ccgcaagatc attattgctg tgcacaagat atacacggac
    20461 gcttccggtt gtctgcggac gataatcaac actgcccacc ggtgccatct tatcaatcgc
    20521 atcgtcaggc agcagaaccg tggcattgat actgttgtcg tttccaactc ctgtaattgt
    20581 caccgttttg ctttgctcat ctgaaacagt tccgctttcg caggtaaaga ccggctgctc
    20641 gctgagcttt gacggaacaa cactacctgc tgcgtttaat gcctcaactt ttacagaaat
    20701 atcagtgttt gaaggggtaa gggttaaagg ctcttcctgc cccaaagtat attctccgat
    20761 ctttagctca ccgctaagat atgtcaggcg gtaagccgct atttttgtgt cgtccggatt
    20821 gacaaaggtc aaatctgatg cgtctacgtc aaaggtaccc acatttcctg cagcatcata
    20881 gaaagtaaag gtgtgtgttc tggtatcgga cattgcatca aagcttaccg atgatgcacc
    20941 gattaccgta acatcctctt cgctgaaccc ggttaaagaa taaactgcag aatagaggta
    21001 aacattgcct gccgcatcct ccgtgctgtc aaaggccacc gaaacatttg cctgcggagc
    21061 ggttttatcg atattggtaa taggtatgct atagctctga gcacttccgc cgtctgcagg
    21121 tgttatggta taggttacat tgccgttttc ggtcatcttg gttttgtaag tgcttacggt
    21181 aaaccctttt gacggaatga tctcaatggt gacggcaccg tttgtcggca ctgtcgggct
    21241 tactgtaaag gtatgactgc cgccgaatat gtcggcatat accttttggg taaaataccg
    21301 attgaatatg tcggtatatg tgattatgac cacaccgtcg gaatagatgg gcaaactgct
    21361 gtgggccgta ttaagtgagg tattgaattc agtcagcttt acaggcacag taaagctcaa
    21421 attctctgcg tatttgtaaa ccggcacctt tttatccggg tcaattaaat tcgttatttt
    21481 cggctcgatt cctgccaggc cgccgtttgg cttccattcg cttatagcag agttaccggc
    21541 ctcatcctca ataatcaatg tggcttgggt catcttgatc ttttcatcgc tcataatata
    21601 tatttttccg ttaatttcat gggtagtttt attaaatttt ccgtcaaact tacccatgac
    21661 attgtcaaac tcaaaggctg gaatatcggt gtccttttcg taggtttcac cggtcaaaag
    21721 ctctgtgtat gccttatcaa acttaatgta tgctcgagat acatcactgc tcaccttggc
    21781 cgatatatcg aagctgccgt cagcatttgc tgtaaataaa ggctccgatg taaattcggg
    21841 cggttcgcgg tcaatattgg ttatatggta tacggcataa gtgtcaagcc ggttaatatc
    21901 atcctcatac tttggtgttt ctgttccgtt aactataagg gtttcgtttt tattgtccgc
    21961 cctatcggat gctattgcaa caataaagcc attgcccgtg aaggtatagc cgccgtcttc
    22021 gtcgggagtg aggaaaaaac ctggattata gtcgctataa tagagctctt cgacattttg
    22081 cggtgttccg aactgtaccg catagggcgt atcatacatc atttccacat ccgactcatc
    22141 cgtgacgctt gtaatcctta cataaacttc acttgccggc attgaattat caaccgatgc
    22201 agacagctcg actactggcg gtgtgttgtc ctttttcagc ttaacagcat ttatcgaacc
    22261 ttcgatgttt cgggcaatgt ccacaagctg gtagtaaact atagcttcgc cctcttcatc
    22321 aagggaaccc accggaagat taccctcaga aacaaactca aagcttccgt cgcttgattc
    22381 ctcaccactg tttaaaatgg ttggatttaa aattttgccc tctgaaacca aagttccaac
    22441 acgcataaca agtccggttc ccagtaagcc tcttccttct gtatcaaggt aatctgccgc
    22501 tattctttgc cgtgcagtta gttcatggag cagggtatat acgttttgag ccttgatata
    22561 caaattaatg gtactgtttt cgttaaaagg caccacaggt acatttgata ccaattcatc
    22621 accgttaaac acggcaagag caccaaattt tgtatatggt gaatcatact gtgtattttc
    22681 gaattgtaag agcttaggac tactatccct ctgcaaaaga ttatactgta tgctttcgta
    22741 tctccctatt tcccatccgt tgttatcaaa cacaaactta gctgttgtaa atgtttcaga
    22801 tgcattgttc acataagtgt attttgtacg gaactcgtag ctgattagat attccatacc
    22861 gtactcatcg atttcatagg ctatcttgtc gatggtgccg gggtcgaagc tcactgtata
    22921 ggagtaaaga tttttggata tattcccgat atgttccata cggctttctt cgccctcatt
    22981 tgttccgtag tacagcatat ctcccatggg agtaagactg tttttatcta tactccatgt
    23041 gctgacagtt tctacgtcat aaggcaagtc gtatatcggt tttttaacct tcaccaaact
    23101 gacttcaata tccttcaccc ggtcaagacc cgttacattg tcatatccca aggcaaattc
    23161 aacctcagca aagccgtaaa tattaggcat attatacgaa cgagactcgt ctgcccatcc
    23221 taagccgggg cggttaaaat attgactgtc attttcgaaa tagtttatgt aagactcata
    23281 gcgtttttca agatctagcc tgtaggtatc ctcacgtaca ccaaagcgat tatttgtaga
    23341 aaatcgtatg gggtcattgg agatttcggg tgtgttatag aaagtgtcaa aaggtatgga
    23401 ttccacatgc catacatcat cgctgtctaa atatgcaacc aaaagctcta aaggacgggt
    23461 ggtatcagat atatccagca tatctgctgc atttaaggag ataccgtcgc ctgttccctg
    23521 aaagcctgtt ccgccttccg ttaagctgcc catcttaaga tgcttgagaa ccttcgtgct
    23581 ttcccctgcg gttattgata tttcttcttc attctgatag ccctcaccct cagggtcttc
    23641 gtcggtcaat tcaccgctaa atgctatgat atttccttct ctgcggtaat atatccccga
    23701 tgcattatct gcaaggcctg cctttacata atcggcggca gtgcttcccg aaagagcgcc
    23761 ccaagtggca gtccatgcac ctgtggtctc cgcccatgca taccaatacg ccttggcgtt
    23821 ttggattttg attgtagctt cgggataaac atcctttgtc aataaatccg tattatttgc
    23881 tgtaattaca ttcacatcag aacaagtgtt gttaaagctg aagttcattt gtgatcttcc
    23941 gatattgccc tttttatcgg cagctttgac ccataatgtt ttttcataaa tcttattttc
    24001 atttaaatcg tttgccgtgt agcgaatttc tgtgcccgtt tcactataag aagcattatc
    24061 cggctcgcta tcttcctcat ctgtccatgc gtatgtaacc gttgtgccgg agatatccaa
    24121 aacatcaact gcggcataat tattataggg ctttggtgca tctatatcat attgttgtgt
    24181 aagagtaatc ctcggtgaaa gggtatcgta gccaataggc agcgttttcg ttttcgtgcc
    24241 tgagtttccc gcttcgtccg caaccgtgac agtaaccgtt gcggcatcaa actcagcagc
    24301 ctgtggcaat tttacaagca tataagcctt gccttcaacg atcggagcat ctgcactaac
    24361 catcgtgccg gtttgtgacc aattgttatc atcatattcc aggctgtttg agggcttata
    24421 tcccagtgtc ccgtcaatct taccgttaaa cgaaatatta aagcttgcat agcaaccctc
    24481 aaggtttgca tcatcgacat tcgccgtaaa aacagccaca ccgccctcca atgcggcatc
    24541 cacatctacg gaaggctcct taaagtccag tttataccgg tgtgctggta ccacgccctt
    24601 cataagattt ttagcatctc tttgttcact ttctttaatg atagacggtg catttgccgg
    24661 cacaagcata tttcccgttc tcttatattt caaaagctca taaccgcttg catcctttgt
    24721 tccggcattt gggtttactt ctataacttc aattctgtcc tcttccaaaa atgtgtgtcc
    24781 cggtacagcc tgataatagt aagtaatact cttgccgtca ctgctaatca atgcatttgg
    24841 agcttgtata taggaatatg gatccgcagg atttataatt cgccatgtac cggcataaga
    24901 ttgactgctg ctttgtgtcc actcttgggt aatactgctc ttataccatc ttttaaggaa
    24961 atcatcataa gctgtcggcg taatggtgat atattcaccc tctttgtctt ttatattcaa
    25021 agtaatgctc ggaagctttg tataatcggt atatttaagg gcaaggggct tgttgaaagt
    25081 aagctgaatc ttaataagct cggactgatt tataaattct ttgccggtat ccgtctccga
    25141 ctccctataa atgtcaatcc ctgcggtttt ttcgccgaag gctattgaca gcggcagaat
    25201 atcaacctta ccgttcatcg ccacagagta gggagtgggc tcgccttctt tgctcgtgct
    25261 tgccacattg ccgcttatat ctctcatttt gttaaacaaa acgggattat cgcttgcatt
    25321 aatattcacc ctggtgaaac tgtataaaag tccccgttcc tgagtaactg aactgttatc
    25381 ggtatacggg tccggtactt gatattcgaa aatcatttcg gtttccgaca ttgatgagtt
    25441 tcctgtttct tccggagcat aatgcaaaaa tgacgccttg gctaaaaggc cttcagtgcc
    25501 gtccatgccc aaagtgcgaa tatttaaagc tacatcctta ggatccacat cattttcaaa
    25561 cttcacaggc tcacttaatg ttaacttgaa ataaattgtt ctgtcgggat gcttcgaaag
    25621 ccagtcaaga gtgataacat ttcccggtat ggcattacca cctgagtcgg tcatgattat
    25681 agactttacc atcggtcctt cggtatcccg tccgagaacc atacccatgc ccagcttcgc
    25741 ccggttattg ttgtcaaaat cgttatcgtt gctccgccac tttaaatccg acatcttcat
    25801 gcttgatttc cagttgctgt ttttggatat atcataaccg gaatttttcg ttccatggta
    25861 gtagtcgtca ttttctgtgg aatgggaaaa acccataaag gaaaagctgg ctttctccca
    25921 ctggttataa aatgtgataa aaccggtttt tttgttccag ccccggatat tgccgccaaa
    25981 ataatactca tactgatttg cgctgggaac atcaaacttg taggacgcgt tgtaattctt
    26041 tagtccgctg atttctccgt tgaaggtttc gggtgcttta gcagtattgc ttgcagatat
    26101 acgttcgcca aagctgttga agctgtagcc gctttgaata agcttggtaa actgatcgac
    26161 catactggat ttactgcccg aagcggccgg aaactgagac agggaatagt caaaaaacat
    26221 cacggttttg cctttgtact tcaaagaagt cagcggctca taggtcaccg gatggccgta
    26281 actgttcgag tcctcagcca gtactgttgc cggcagcagt gtaagaatca gcaccgccgc
    26341 gagcagcaga gacaggggtt ttttccccca agtgtttttt ttaatcataa ataaactccc
    26401 ctcttatttg ttatatatgg atgtattatg taaacgtgaa gtaaaatatg cacatgttat
    26461 atataataag gctctgatgt ggtgtccttt tgcttttttc ttttttgttc catgccctaa
    26521 aagaaaatct cctcgggcat caagccccca tcgcttttta ctcatttcac ttcgtggaat
    26581 cctactaagg actataaaag cacccgcatg cgggtgctta tgtaagctga tta
//
LOCUS       DAOOJC010000002        18265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_3009_length_18265_cov_3.782592, whole genome shotgun sequence.
ACCESSION   DAOOJC010000002 DAOOJC010000000
VERSION     DAOOJC010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 18265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18265
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3009_length_18265_cov_3.782592"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PLA01_00085"
     CDS             complement(<1..653)
                     /locus_tag="PLA01_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HPC99729.1"
                     /translation="MNVLLIRPKPHGETIGLQSVMICEPLEFMCLASALKQNGHAVDI
                     LDMIIEKKPLGHFIEKFNPDVVGITGYISHVGIIKKYAKVIKSYSKDIKVSVGGVHAK
                     VCPEDFKDDNIDIVCRTEDDLYDFLDCKIREKTFPDRKITERYQDKYYYLFQNKCALI
                     KTSFGCPYSCNFCFCKEISPYSAREIDDVIAELKTVPQKEVYIVDDDFLFNRQRLLE"
     gene            1090..2421
                     /locus_tag="PLA01_00090"
     CDS             1090..2421
                     /locus_tag="PLA01_00090"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC99730.1"
                     /translation="MEASKKIKVLLVRPKFSSIVANLEPLGLEYVAGLCRDLNIECEI
                     FDEFQYSWHFRFDRLSRKIEEGGFNIVGFHSNANTSDYIRNTSDKLKKKFPGLYIMVG
                     GPEAELNYRDFFTNSIDFVYHNNGLISLKNILLEGFSPEVLNKQNGVCFKINNKWVVK
                     EKGEPVNNFITRPERTAFYKGLKKNFIFLKGTFAITKASFSCPFDCTFCYCTKMNNGV
                     YTERPIMEVVEEIEAIDHPRIWFVDDTFLVDKERVKKFCETIIERKIKKHFMAYSRAD
                     FLAENPDILPLLYKAGFKDILVGLEAVSDDFLDDYNKQTSKDVNTRAVKNLLDNNIVC
                     NGLFVVSHKSTRQDFKELYRFIKKNNLLWVVFGIFTPYKGTDAYDEYKDRLVKFKSRR
                     LDGIHITIKPEKMSSFMFMVRVYMLYVLTYPKIFCRTLFKTAYDTKRTGWI"
     gene            2441..3769
                     /locus_tag="PLA01_00095"
     CDS             2441..3769
                     /locus_tag="PLA01_00095"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPC99731.1"
                     /translation="MAKQKYLLVRVRYDKRVASIEPLGLEYLAGVMKEKGREYVFHDE
                     IFYSSFFRFRRIVKKIEQNNITAVCFSVMSNNADYILKMINKLKKVKPDIKILVGGPE
                     VNINYKDFFLDNIDFVYYDYGLDSFRIAVENDLNVDALEGATGIAYVKDGKWVHNPCG
                     KPITDYDVKPDRSLFYENRKKYRVIAKGCFSLMKSSFSCPQECNFCISRQFNGCFYAE
                     RNTDNVVNEIIEIDNNRIWLCDDDFLVNKERVIEICNKLIERNCYKTYMIFARADSIV
                     KCEDIMPLLYKAGFRDMLVGLEAVEDKILDSYNKKSSVEINEKAVKILRDNKMVCNGL
                     FVINYDFKNKNFIDIHRFIRKQKLVWVLFSILIPFKGTRVHEVNKHKLYKYRYGRTDG
                     TKLLMRPQNIPAALFKVHFALLYYINFPRIYLAYFLKTYDKKYLNKVQNQ"
     gene            3784..5139
                     /locus_tag="PLA01_00100"
     CDS             3784..5139
                     /locus_tag="PLA01_00100"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPC99732.1"
                     /translation="MRVLLIKPETIGIFAYTNLVDHEPLELEYLYTILKNRGHDAYIY
                     DRRHDMTPLRRKLKTFTPDVVCITGYITQEKLMIKLARLIKRFNSNITVIIGGSHAEL
                     NYKNFIDSPVDYIYHLSGLDSFIKLIDFIDNTSSTPLDDIPGICYRKGHGWNINKKVV
                     ETPEDLPPIDRTYFYRNRNRYRYLTFHPLALVKNSYSCNSSCTFCYCTNRNSGQYACR
                     KAEDLVNEIMATDAPNIHITDDNFLVDREYLKQFVELIREKKINKKYLVYGRADFIAE
                     NKDIMCELKDIGLSLVMVGLEARSNQELDSYNKNATLEHNEECVRTLEELGIICAGLF
                     IVHQDMTVKDFKELYNWIDQRAIIPTISIFTPMQGSADFSKYKDNLLSTDSRKQDLFH
                     CILKPKQMSTFRFYYEYYKLSIKLAWNRRKAPLYSCINIGSFFFIIKVLLMKLRRVFI
                     I"
     gene            6001..7617
                     /locus_tag="PLA01_00105"
     CDS             6001..7617
                     /locus_tag="PLA01_00105"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPC99733.1"
                     /translation="MSVKYIFVTGGVVSGLGKGITAASLGRLLKARGLHVTIQKFDPY
                     INVDPGTMSPYQHGEVFVTDDGGETDLDLGHYERFIDENLNKNSNVTAGKIYWSILSK
                     ERKGDYLGGTVQVIPHVTNEIKDRIYRVGKSGSTDVVITEIGGTVGDIESLPFLEAIR
                     QVATDVGRENVMYIHVTLVPYLGKSGELKTKPTQHSAKELRSIGIQPDVIVCRTEKRL
                     SRELKDKIGLFCNIPGESVIQNLDAEVLYEVPLMLEEEGLANIVCKRLSLQCQEPDMS
                     EWCQMVSKQKNLSKKVTIALVGKYVELHDAYLSVVESLNHGGIANDAEVEIKWINSEN
                     LNPKNLKDSFSGINGILVPGGFGDRGIEGKILAAKYARENKVPYFGICLGMQMAVVEF
                     ARNVAGMENANSSEFDLETKYPVIDIMPDQKDLDDKGGTMRLGIYPCRVIEDSKIFDI
                     YQDKLIYERHRHRYEFNNEYRDVLTSKGLVISGVSPSEKLVEIIELKDHPWFIGVQFH
                     PEFKSRPNRPHPLFRDFIRAAVENKEANGN"
     gene            7815..8537
                     /gene="spoIIR"
                     /locus_tag="PLA01_00110"
     CDS             7815..8537
                     /gene="spoIIR"
                     /locus_tag="PLA01_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HPC99734.1"
                     /translation="MFIFLRRSKPRKIKSMKLFSKTAAAVVLLGTLILAIAFVSYSEN
                     VSNGLADNLVRLHVVANSDSPEDQQLKRDVRDVVIEYMKHKLKDAKDIEAAKKVIVEN
                     SGEIKRIAENEIIRQNKDYNVDAMLGTYPFPTKVYGDISLPAGNYQALKIVIGNGEGQ
                     NWWCVLFPPLCFVDATHGTVPDTVKDDLKEVLTEEEYCLVTSVGNEEDIPIEIKFKVV
                     EVFQDSKIKLAGAMNRLFKSLF"
     gene            8773..9462
                     /locus_tag="PLA01_00115"
     CDS             8773..9462
                     /locus_tag="PLA01_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HPC99735.1"
                     /translation="MLGMIIENGIYYIVVLARLLLACFLGGLIGYERESTNRPAGFRT
                     HILVCVGASLIMITAESISQKYAGLTNIDPTRLGAQVISGIGFLGAGTIIREGVNVRG
                     LTTAASLWAVSCVGIAAGIGFYGGAIIATAIIYITLILLKRTEKHIGRKNRLSLFRIR
                     TGNLPGQISTIGCIFGKYDVTIKNIEFLNAADENEIHIKYFVRIPGSVSPEKLMDELQ
                     GLEGIKDVSQE"
     gene            9694..11151
                     /locus_tag="PLA01_00120"
     CDS             9694..11151
                     /locus_tag="PLA01_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPC99736.1"
                     /translation="MARLDQYKTPLFDAVKRYVDSNVIPFHVPGHKQGAGLAEFREYI
                     GERALQMDANGMEDLDFANNPTGVIYESEVLMAQAFGAQNAYFLVNGTTAGVQAMIMS
                     ACEPGDKIIIPRNAHKSTIGGIILSGAMPVYVQPEVNEKLGIAMGITEESLRKAIKEN
                     PHAKAVFIINPTYYGFVSDLKSLVRIAHRHEMAVLVDEAHGAHMSFHDGFPLTAMEVG
                     ADMSAASIHKTAGSLTQSSVLLLRGDMISPERVKQVLNLTHTTSASYLLMSSLDIARK
                     QLSTKGSDMLEETLRLARMAREEINKIEGLYAFGKELTGTPGCFDFDETKLGINVLGL
                     GFTGYEMEAKLRKEYNIQIELSDLSNILAVISIGDREENVVALINALKDISSKTEKKE
                     YTNPPVIPQNPKMIVSPRDAFYSHKKIVPLEKSVGEISGEMVMAYPPGIPVICMGERI
                     TQEIVDYINILKEQKTQLQGTADPYINSIMVLGAD"
     gene            11536..14256
                     /locus_tag="PLA01_00125"
     CDS             11536..14256
                     /locus_tag="PLA01_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPC99737.1"
                     /translation="MESTVKSFNKDDRYKYGLSEKEAEKKFEKYGPNTLTKKKKISPF
                     KILIDQFSDFMVLILLASTLISAFMGEVTEAITIISIVVVNAILGFIQEYRTEKTLEA
                     LKNLAAPTAKVVRNGKTVEIPAERIVPGDLIIIEAGDRVPADAVLTEANGILADESLL
                     TGESVPVEKFVEVNGKRNGAAIEKNSSLYMGTIVTSGRGKAIVCATGMLTEMGKIADM
                     IQNIETEETPLQKRLDSLGKYIVYGCLAICAVVSITGVLRGQKLFTMLLSGISLAVAA
                     VPEGLPAIVTIALALGVRRMLKRNAMIRKLPAVETLGCASVICSDKTGTLTENKMTVR
                     KIYSGGGYTEVRGSAMSSEGEFISFNRKIDPLDNSGLRQTLEIGVVCNNASMVRIKEK
                     NPFGKISSMVSKQEKWELSGDPTEAALLVAGAKGGLTQESLGEKYFRIDEIPFDSDRK
                     CMSVICENKNGEIFVFTKGAPDVIINKCSKIYTSKGIVNLDGEGTRKVLKANDSMADE
                     ALRVLGVAYRKLGSRFYKNSEVEKDLIFVGLIGMIDPPRKEVLEAVRKCRLAGIKPVM
                     ITGDHKITAAAIARELNIATAGDRVVTGAELEQMDDNKLQEVVEEVSVYARVSPRHKL
                     RIVRALKKRGHIVAMTGDGVNDAPAIKEADIGVSMGITGTDVTKESAAMILRDDNFAT
                     IVAAIEEGRVIYSNIRKFIRYLLSCNIGEVFTMFVGMLIGFPLPLLPIQVLWVNLVTD
                     GLPAIALGFEPAEKDIMMRPPRGAKENIFSQGLLNLILFRGTIIGVCTLAVFASIFHF
                     TSSVETARTAAFVTLVMTQLMHVFECKSERKNIFEVPFFNNIALVLATMCSLTMILSA
                     VYIPFMQEIFKTVPLSVNDWILIAGFSAVGPVVSSFFRVNARYIRGHIYK"
     gene            complement(15238..15732)
                     /locus_tag="PLA01_00130"
     CDS             complement(15238..15732)
                     /locus_tag="PLA01_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPC99738.1"
                     /translation="MKKGSPAWGLVFIFIGGLLLCDSIFDVNIFSISRLWPMIILVLG
                     LIFEFSYFVGKKSPGLLVPGGILTVTGFLFLFESFTGWYFSKYTWPIYIISVAFGLFQ
                     LYLFSGKPKGLLIPISVLTTVSVVSLSIIILNIVTSSINFGLLIPLAFFALGGYIIYN
                     SLRN"
     gene            16009..16695
                     /locus_tag="PLA01_00135"
     CDS             16009..16695
                     /locus_tag="PLA01_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPC99739.1"
                     /translation="MGAKVLTVEDDNSIRGFLVTILKRNNFIVSEAVTGKEALRKVEL
                     EKPEVVLLDIMLPDIDGFEVCKKISQQHPDIPIIMLTARGEDTDKINGLELGADDYII
                     KPFNPMEVIARIKAIIRRTSKTDFSNEIEIGPFKIDLNCQGVYKNGQVLDLTPTEFCL
                     LKVFANNVNKSLTRDDILNWVWGENYVGDTKTVDVHVRRLREKIEDDPSKPKYIETVW
                     GHGYMWRKGD"
     gene            16667..18088
                     /locus_tag="PLA01_00140"
     CDS             16667..18088
                     /locus_tag="PLA01_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPC99740.1"
                     /translation="MGICGGKEINVRGVRGRIAGTYLIITLLAVLIFEAILIYILVRF
                     YYTNIESELNASANTIVHNYEVYFNGDDLDRDAKLLMESVSSNTVAQIQIIDREKVVL
                     VDSINPVSVGESLDYPDINGALKGKKTTWRGRNPQTNESILSVSSPIKKNEEVIGVVR
                     VITALTDVNKILFNHVLTLIVLGTFIVILIFVAGVFLSNTIIKPVKEITCAAEAMARG
                     RFDVRVNKRYDDEVGKLADTLNYMAEAVSKHEKMKNDFIASISHEIRTPLTSIIGWVI
                     AINSGNVENSEEIREGLGIVERESERLAAMVEELLDFSKFDAGSINLKIDLVDLKELI
                     SHITKQMEPRAKREGIFIVMRTDEDLPFIDADENRLKQVLINIIDNSFKFTQKGGYIN
                     IIAKQLKDKVLICVEDNGCGIPEDDISKVTQRFFKGNSNAAGSGLGLAICDEIIKLHN
                     GEMNIESVVEKGTAVNIILPVRA"
BASE COUNT         5938 a         2922 c         4039 g         5366 t
ORIGIN      
        1 aattccaaaa gcctttgccg attgaacaaa aaatcatcgt caacgatata gacctctttt
       61 tgaggaacgg tttttagctc ggctatcaca tcatctattt ctcttgccga atagggcgat
      121 atctctttac aaaaacaaaa attgcagctg taaggacatc cgaaggatgt tttaataagt
      181 gcacatttat tttggaaaag ataataatat ttatcttggt atctttcggt tatctttcgg
      241 tccgggaagg ttttttccct aattttgcag tctaaaaaat catatagatc atcttcggtt
      301 ctgcaaacaa tatcaatgtt atcgtcctta aaatcctccg gacatacttt ggcatgaacc
      361 ccgccaaccg agacctttat atcctttgaa tatgatttga taacctttgc atattttttt
      421 ataattccca catggctgat atatccggta atccctacta catcgggatt gaatttttca
      481 atgaaatgcc cgagaggctt tttttcaatt atcatgtcta aaatatcaac tgcatgtcca
      541 ttctgcttca acgcagaagc aagacacata aactctaacg gctcacaaat cattacactt
      601 tgaagcccta tggtttcccc gtggggtttt ggtcttataa gcaaaacatt catatctttt
      661 cccttatatt aaaaaatgtt atatcttttc actagtttac tccataataa ctcaaaattc
      721 aacaaaaaaa acctgccact agggtgcatg aagccatatg aaaaaatatt ccagaaactt
      781 aagttacgca agtccagagc ctgtaaatac tcctcggggt cgagcaaaac tcgcttcgct
      841 cagacaatgc tctccctttc ctcgtcgtat taacaggcta tataacttgc ttcacaatgt
      901 ttcttccata ttttttcatg gcctcatgca tccattcaaa cgagaaactg aaataataat
      961 atggaaacta tggatacata taaataagat gtgccacttt ccatcatacg acattttgtg
     1021 atacaattag gttctgattg gagaataaaa aaagtataag cactttataa atttcacagg
     1081 gggtaagtga tggaggcttc aaaaaagatt aaggtcttac ttgttcgtcc caaattttca
     1141 tccattgttg caaatttaga accgcttggg cttgaatatg ttgcaggact ttgcagggat
     1201 ctgaatatcg aatgcgaaat attcgacgag tttcaatatt cctggcattt cagattcgac
     1261 agattatcga gaaaaataga agagggcgga tttaatattg tcggctttca ctccaatgcc
     1321 aatacttccg attatatcag aaacacatcg gacaagctca agaaaaagtt tcccggtctt
     1381 tacatcatgg tcggaggtcc cgaagccgaa ctcaattata gagatttttt caccaatagc
     1441 attgattttg tatatcacaa caatggcctt atctcattga aaaacatcct tctagaaggc
     1501 ttttccccgg aagttttaaa taagcaaaac ggcgtttgct ttaaaataaa taataaatgg
     1561 gttgtaaagg aaaagggcga acctgtaaac aacttcatca cacgcccaga aagaactgcc
     1621 ttttataaag gtttaaagaa aaattttatt ttcctcaaag gtacctttgc tattaccaaa
     1681 gcatcctttt cttgcccttt tgactgtact ttttgttatt gtacaaaaat gaacaacggt
     1741 gtatataccg aacgccctat aatggaggtt gttgaggaga tagaagccat tgaccatcct
     1801 agaatatggt ttgtagacga taccttcctt gtcgataagg aacgggttaa aaaattttgt
     1861 gaaacaatca tagaacgaaa aattaagaag cattttatgg catattcgag agccgacttt
     1921 ctggcagaaa acccggacat actcccgctt ttgtataaag caggttttaa ggatatttta
     1981 gtggggcttg aagcggtaag tgacgatttt ttggacgatt ataataaaca gacctcaaaa
     2041 gacgtgaata cacgggctgt taaaaatcta ctcgacaata atattgtatg taacggcctg
     2101 tttgttgtaa gtcacaaatc aacacgtcag gatttcaagg agctataccg gtttataaag
     2161 aaaaacaatc ttctatgggt ggttttcgga atctttacgc catataaagg caccgatgca
     2221 tatgatgaat acaaggaccg tctggtaaaa ttcaaatccc ggcggcttga cggcatacat
     2281 attacaataa agcctgagaa aatgtcctcc tttatgttta tggtgagggt gtatatgttg
     2341 tatgttttga catatccgaa aattttttgc cgcactctat ttaaaacggc atatgacacc
     2401 aaaaggaccg gttggatcta agatatggag gtatgcataa atggcaaagc aaaaatatct
     2461 tttagtaagg gttcgttatg ataaaagggt ggccagtatt gagcctttag ggcttgagta
     2521 tttggcggga gtaatgaaag aaaagggcag ggaatatgtc tttcatgatg aaattttcta
     2581 cagctcattt ttccgtttcc gccgcatagt taaaaaaata gagcaaaaca acataacggc
     2641 tgtttgtttt tccgttatgt ccaataatgc agattatata ttgaaaatga ttaataaact
     2701 aaaaaaggtg aaaccggata taaaaatttt ggttggcgga ccggaagtaa atatcaatta
     2761 taaagatttc tttttggaca atattgattt tgtatactac gattacggtt tggattcctt
     2821 tagaattgct gtcgaaaacg accttaacgt agatgctttg gagggtgcca ctggaattgc
     2881 ctatgtgaag gacgggaaat gggttcacaa tccgtgcggt aagcctatta ccgattacga
     2941 cgtcaaaccc gacagaagcc tgttttatga aaaccgtaaa aaataccgtg ttattgccaa
     3001 aggctgtttt tctttaatga aatcatcctt ctcctgtccc caagagtgta atttttgtat
     3061 ttcaaggcag tttaacggct gtttctacgc cgaaagaaac acagataatg ttgtaaatga
     3121 aataatagaa atagataaca accgcatatg gctgtgcgac gacgatttcc ttgtcaacaa
     3181 ggagagggta attgaaatat gcaacaagct tatagaaaga aactgctaca agacatatat
     3241 gatatttgcg agagcggata gcattgttaa atgtgaagat atcatgccct tgctgtacaa
     3301 ggctggtttt agggatatgc ttgtgggctt ggaggctgta gaggataaaa tactcgattc
     3361 ctataataaa aagtccagtg ttgaaataaa cgaaaaagcg gtaaaaatct taagggataa
     3421 caaaatggta tgcaacgggc tgtttgtaat aaactatgac tttaaaaata aaaactttat
     3481 agacattcac cgctttatca gaaaacaaaa actggtctgg gttttattca gcattttgat
     3541 accctttaaa ggaacccgcg tccatgaagt aaacaaacac aagctataca aatacagata
     3601 cggaagaacc gacggtacta aactgttgat gaggccgcag aacatccccg ctgctttgtt
     3661 taaagttcat tttgccttgc tgtattacat taattttccg aggatatacc tggcttattt
     3721 tttgaagact tatgacaaaa agtatttgaa taaagttcaa aatcaataag ggggattcta
     3781 taaatgaggg tattgcttat taaacctgag accatcggaa ttttcgctta taccaatctt
     3841 gtggaccatg agccccttga gttggaatat ctctatacga tacttaaaaa tcgcggacac
     3901 gatgcatata tttatgacag aaggcatgat atgactcctc ttaggagaaa gcttaagact
     3961 tttactcccg atgttgtgtg cataacggga tatataactc aagaaaagct gatgataaaa
     4021 ttagctcgcc ttataaagag gtttaattca aacattactg taataattgg gggcagtcat
     4081 gcggagctga attacaaaaa tttcattgat tcgcctgtgg attacatata ccatttgagc
     4141 ggattggact cttttataaa actcatagat tttatcgata ataccagctc aactcccctg
     4201 gacgatattc ccggaatatg ctacagaaag ggacatgggt ggaatataaa caaaaaggtt
     4261 gttgaaaccc ccgaggattt gccgccaata gacaggacat atttctaccg aaatagaaac
     4321 cgttaccgat atttaacttt tcatcccctt gccttggtca agaattcgta cagttgtaat
     4381 agctcttgta ccttctgtta ttgtaccaat agaaacagcg ggcaatatgc ctgccgtaag
     4441 gcagaggacc tggtaaatga gattatggca accgatgcac ccaatatcca tattaccgat
     4501 gacaattttt tagttgacag ggaatatcta aagcaatttg tagagctgat acgcgagaaa
     4561 aaaatcaata aaaagtactt ggtttacgga agagcggatt ttattgcaga aaacaaggat
     4621 attatgtgcg aactgaaaga cattggactt tcactggtta tggtaggcct tgaggcccgt
     4681 tccaatcaag aactggactc gtataacaag aatgcgacat tggaacacaa tgaagaatgc
     4741 gtcagaactc ttgaagaact cggtattatc tgtgccggtc tgtttatagt tcatcaggat
     4801 atgactgtaa aagacttcaa agaattatat aattggatag accagcgtgc tataattccg
     4861 actatatcta tttttactcc catgcaaggt tcagcggatt tttcaaaata taaagacaat
     4921 cttttaagca ctgattcaag aaaacaggac ttgtttcatt gcatcctaaa accgaagcaa
     4981 atgagtacct tccgttttta ttatgagtat tacaagcttt ctataaaatt agcctggaac
     5041 aggaggaaag ctccccttta ctcttgcata aacataggat cgtttttctt tataatcaaa
     5101 gtcttattga tgaaattaag gcgggttttt attatttaaa aaaataaaca taaattttga
     5161 tagcgtattg acatactttt tcataaactg tataattatt gtaaggggaa ttctatttgt
     5221 atcagcggtt gtttttactt atataaaatg gggaaatgct ttaaatgaag cagttttaac
     5281 gactttaata ttttattctt aatttgctcc attctttttt gtacagtaat tattacataa
     5341 atttattttt atatggcttt aggacaaaca tgaaggaagg aagtgaggct gttaacttag
     5401 tagtccaaag ttcaaaataa tgatgagttt taaattttgt ttaccttcta ttatatggaa
     5461 ttatatgtgg gagacagttt ttggtttatg gatttttact ttaactagct tgtgtattta
     5521 aggataaaaa gtttagtaaa ggtttagggg agagtatata tttttgacgg tacattaaat
     5581 gactgggaga gttcttggag gtttttaggg gaggcacaag gcggttttga gtgtttacag
     5641 ggctttggac tactaagtta gcagcattca tatttttatt aatattagat taacattcat
     5701 gaaatgaaat ctatatgagt catttctttt tttattttca caaaacggca tattatgaca
     5761 caagttggat gctaagcatt tttattgttt aatttctccg ggtataagaa tttttatatc
     5821 aaaaatatgc tccgaattga gaaataccta ttgacactgt attatgtcat atataaaata
     5881 taagcgtgtg ttttattata ttaaaaaaat ataataatca ataaagtgtc aaaagtatta
     5941 attattcagc cgtgaagtgg ttttcccggc tgtagttgtt tttagtggag gattggaatc
     6001 atgtctgtta agtacatttt tgtaaccggt ggtgttgttt ccgggcttgg taagggaata
     6061 accgccgctt cgctgggaag gcttttaaaa gccagaggtc ttcatgtgac tattcagaaa
     6121 tttgaccctt atataaatgt cgatcccggt actatgagtc cgtatcaaca tggagaggtg
     6181 tttgtaaccg atgacggggg agagacagat ctggacctgg gtcattatga aaggtttata
     6241 gatgagaacc tgaacaaaaa cagtaatgta acagccggaa agatttattg gtccatactg
     6301 agcaaagaaa gaaaaggcga ttatttaggc ggtaccgtac aggttatacc ccatgtaacc
     6361 aacgaaatta aagacaggat ctacagggtc ggtaaatccg gcagcacaga tgttgtaata
     6421 accgaaattg gaggtactgt gggggatatt gaaagcttgc cgtttcttga ggccatacgt
     6481 caagtggcaa cagatgtggg cagagaaaat gtgatgtaca tacacgtcac tctggtgcca
     6541 tatctgggga aatccggcga actcaagacc aaacctactc aacacagtgc caaagagtta
     6601 aggagtatag gtattcaacc ggatgttatt gtttgccgga ccgagaagag actatcgagg
     6661 gaattgaaag ataaaatcgg tctattctgt aacattcccg gtgagtcggt aattcagaat
     6721 ctggatgcgg aagttcttta cgaggtgcct ttgatgcttg aagaggaagg cctggccaat
     6781 atagtatgca aacgtctcag cctccaatgc caggagccgg acatgtctga atggtgtcaa
     6841 atggtaagta agcaaaagaa cttgagcaag aaagtaacca tagccctggt cggaaagtat
     6901 gtggaactgc atgatgccta cttgagtgtt gtggagtcgc tcaaccacgg gggaatagcc
     6961 aatgatgccg aagttgagat aaaatggata aactcggaaa atctgaaccc caaaaatctt
     7021 aaagacagtt tttccggtat aaacggtata cttgtacccg gagggtttgg agacaggggt
     7081 atagaaggaa agattcttgc tgcaaaatac gcccgtgaaa acaaggtgcc gtatttcgga
     7141 atttgcctgg gtatgcaaat ggcagttgtg gaatttgcac gcaatgttgc cggaatggaa
     7201 aatgcaaaca gttcggagtt tgatttggag accaagtacc ctgttattga tatcatgccg
     7261 gatcaaaaag acctggatga caagggtggt accatgagac ttgggattta tccgtgcaga
     7321 gtcattgaag actcaaagat ttttgatatt tatcaagata agctcatata tgaaagacac
     7381 cggcacaggt atgagtttaa taatgaatac agggatgttt taacttccaa gggtcttgta
     7441 atttcaggag tgtctcccag tgaaaaactt gtagaaatca ttgaattgaa agaccatcca
     7501 tggtttatcg gagttcaatt ccaccctgag tttaaatcga gacccaacag accgcatccg
     7561 cttttcagag actttatcag ggcagcggtt gaaaacaaag aagcaaacgg caattaggaa
     7621 aaaatatgtg atataaaacc ataatataga ttttgatgaa aataatttta tgtatagtat
     7681 aaatcctttt gaatttgtaa acaattatac taagcaattt aagtctgtaa tgtaaaatta
     7741 gcttcggtat ggtaccattt cgcaatgaaa atttagaaat ggtttactta ccggaatttg
     7801 gggggattta tactatgttt atttttttaa gaagaagtaa accgagaaaa atcaaatcaa
     7861 tgaaactttt ttccaaaaca gctgcagcgg ttgtactttt gggaacactt attctggcaa
     7921 tagcttttgt gtcctattcg gagaatgtga gcaatggact ggcggacaat cttgtaaggc
     7981 tgcatgttgt tgctaatagc gattctcctg aggatcagca gctcaagagg gatgtaaggg
     8041 atgtagtaat agaatacatg aagcataagc ttaaagatgc aaaggatatt gaagctgcaa
     8101 aaaaagtaat tgttgaaaac tcaggagaaa ttaagagaat tgccgaaaat gaaattataa
     8161 gacaaaacaa agactataac gtggatgcaa tgctggggac ttatcctttt cctacaaagg
     8221 tttacggaga tatcagtctt ccggccggca actatcaggc acttaagatt gtgataggaa
     8281 acggagaggg gcaaaactgg tggtgtgtcc ttttcccgcc tctttgcttc gtggatgcca
     8341 cccatggaac tgtaccggat actgtaaagg atgacctcaa agaggtactc acggaggaag
     8401 aatactgtct ggtaacttcg gtagggaatg aggaagatat tccaattgaa ataaagttta
     8461 aagtagtaga ggtatttcaa gattccaaga taaaattggc tggggcgatg aacagactgt
     8521 tcaagtcact gttttaagag taagatctca tatcctccat ggatgtggga tttttttgtt
     8581 agtggaattt ggcaggatga agtcggattt atattacagc tgcatataat tatagaaata
     8641 tattttgagt gataataatg gcatttgctt attgaagata tgcatgaatt tgatatatac
     8701 tctaatagaa ttcaacttat tccatgtaag actttttgaa gctgactaaa aactaaatta
     8761 ggggtgagcc gaatgctggg tatgattatt gagaacggaa tctactatat tgttgttctt
     8821 gcaagactgc tattagcgtg ttttctgggc gggttaatag gttatgaaag agaaagtacc
     8881 aaccgcccgg cggggtttag aacccacatt ctggtttgtg ttggagcatc tcttatcatg
     8941 ataacggccg aaagcatttc tcaaaagtat gcaggtctca caaatataga tcctaccagg
     9001 cttggtgcac aggttataag cggaatcgga tttttaggag caggaaccat tatacgtgag
     9061 ggtgttaatg taagaggact taccactgct gcaagtcttt gggcggtttc ctgtgtaggt
     9121 attgcagcag gaatagggtt ttatggaggg gccattattg ctactgctat aatctatatt
     9181 actttaattc tgttgaaaag gacagaaaag catattggaa ggaaaaaccg gctgagtttg
     9241 ttccgcatta gaacagggaa tttacccgga caaataagca cgataggctg tatattcggc
     9301 aagtatgatg taacaataaa aaatatagag tttttaaatg ctgcggatga gaatgaaatt
     9361 catataaagt attttgttag gattccgggc agtgtaagtc cggaaaaatt aatggatgaa
     9421 ttgcagggat tggaaggaat caaagacgtt tcacaggagt agaggttatt agttgataaa
     9481 aatgtttgac tttttgggta caatctccta taattctctt gtttattatt ttttgataca
     9541 ttgaacacat taagcaaatt attaatgtgt tcaatagctt tgtttatacg gttttgcaaa
     9601 atttgagcag atcttttgga ctaaggcgga tttcggactt agtgaatcaa tgcaaatttt
     9661 tgtgaaacgt atggaagaga gaggggattg ggagtggcaa gattagacca atataaaaca
     9721 ccgttgtttg atgcggttaa aagatatgtt gatagcaatg taattccgtt tcatgttccc
     9781 ggacacaagc aaggagcggg tcttgccgaa ttcagggaat atatcgggga aagggctttg
     9841 caaatggatg caaacggtat ggaggatttg gattttgcaa acaacccgac tggtgttatc
     9901 tatgaatcgg aggtgctgat ggctcaggcc tttggggcac aaaacgctta ttttctcgta
     9961 aacggtacaa ctgcgggtgt tcaggcaatg attatgagtg cctgtgagcc cggggacaaa
    10021 attataattc caagaaatgc acacaagtca acaataggcg gaattatatt aagcggagcc
    10081 atgccggtgt atgttcagcc tgaggtgaat gaaaagctgg gtatcgctat gggtataacc
    10141 gaggagagcc ttagaaaggc aattaaggaa aatccccatg ccaaggcggt atttattatt
    10201 aaccctactt actatgggtt tgtatcggat ttgaaatctt tagtaagaat agcccacaga
    10261 catgaaatgg ctgttttggt ggatgaggct catggtgccc atatgtcctt tcatgacggt
    10321 ttcccattga cggcaatgga agtaggtgca gatatgagtg ccgcaagcat acataaaaca
    10381 gcgggctctt tgacgcaaag ctcggtattg cttttgcggg gagacatgat tagccctgaa
    10441 agggttaaac aggttttgaa tttaacccat accaccagtg cttcctatct tcttatgtct
    10501 tctcttgata ttgcaagaaa gcagctctcc acgaagggaa gcgatatgct tgaggagact
    10561 ttgaggcttg cgaggatggc aagggaggaa ataaacaaaa tagaaggcct ttacgccttc
    10621 ggaaaagagc ttaccggtac tcccggatgt ttcgatttcg acgagacaaa gctggggatc
    10681 aatgttttag gcttgggatt taccggttat gaaatggaag caaaacttag gaaggaatat
    10741 aatatacaga ttgagctgtc ggatctttct aatatcctgg cagttataag tattggggat
    10801 agggaagaaa acgtggttgc actgataaat gcattgaagg atatttcatc taagacggaa
    10861 aaaaaggagt ataccaatcc gccggtcatt cctcagaatc ctaagatgat agtatcaccc
    10921 cgggatgctt tctacagcca taaaaagata gttcccctgg aaaagtccgt aggggagata
    10981 tcgggggaaa tggtaatggc atatccgccg ggcataccgg ttatttgtat gggtgagaga
    11041 ataactcagg aaatagtgga ttatataaat atattaaagg agcagaaaac tcagcttcaa
    11101 ggcacggcag atccctatat caattccata atggttcttg gtgcggatta aaaaatgtag
    11161 cttaaataaa aaggctctac catcaatacg gtagggcttt tttgtacagt ttggggttta
    11221 gtgcctaaat aacgataatt gcaattatct gctaaataac ttggattacc tttatcttat
    11281 cttttcaagt ccctatatat tatcagttta ttaatgaatt tcaaataaca tttgagtaac
    11341 atgttttgtg ttttaaaaag tgaagaacat gatttaaatt ttatactcaa gaagagaaac
    11401 aatggtttat acatggtcta tttatgtttt tttctgaata aaaataggat tatgacaggc
    11461 atattgcagc tataaatccg gcacaagtgc accaggataa ggtattggta aaaggagtga
    11521 ttggcattga gcgttatgga atcgacagtg aaaagcttta ataaagatga caggtacaaa
    11581 tacggtttaa gcgaaaaaga agcggaaaag aaattcgaga agtatggacc caatactttg
    11641 accaaaaaga agaagatttc tccatttaaa attttaatcg atcagttcag cgattttatg
    11701 gtattgatac ttcttgcatc aactctgatt tcggcattta tgggagaggt aaccgaagct
    11761 attaccataa tttcaatagt tgttgtgaac gcaattttag gctttattca ggaatatcgt
    11821 actgagaaaa cgctggaagc ccttaaaaat cttgctgcac cgacagctaa agttgtaagg
    11881 aacggaaaaa cggttgaaat acctgcagag agaattgtgc cgggggattt gataattatt
    11941 gaggccgggg acagggttcc ggcagatgct gtgcttacag aagccaatgg aattttagcc
    12001 gacgagtctt tactcacggg tgaatcggta ccggtagaga agtttgttga ggttaatgga
    12061 aaaaggaatg gggctgccat tgaaaaaaac agctcgttat atatgggaac catagtcacc
    12121 agcggaaggg gaaaggctat tgtttgtgcc accgggatgc taacggagat gggtaaaatt
    12181 gcagacatga tacagaatat tgaaactgaa gagacgccgc ttcaaaaaag acttgacagt
    12241 cttggtaagt atattgttta cggctgcctt gccatatgtg ccgttgtgtc cataacagga
    12301 gtattaaggg gacagaagct gtttacgatg cttctttcgg gaataagtct ggcagttgct
    12361 gcagtaccgg agggacttcc ggctattgta accatagccc tggccttggg tgttcgaaga
    12421 atgctgaaga ggaatgccat gatacggaag ctgccggctg tagagacatt ggggtgtgcc
    12481 agtgttatat gctcagacaa gacaggaacc ctgacggaaa ataagatgac ggtaaggaag
    12541 atttattccg gcggtggcta tacggaagtc aggggaagtg ccatgagcag tgaaggagaa
    12601 tttatttctt ttaacagaaa aatagatcct cttgataact ccggattaag gcaaaccctt
    12661 gaaatcggag tagtatgcaa taatgcatct atggtgagga tcaaagaaaa aaatcccttt
    12721 ggaaaaatat cttcaatggt ttctaaacag gaaaaatggg agcttagcgg tgatcccacc
    12781 gaggcagcac ttctggttgc aggtgctaaa ggagggctta ctcaggagtc tcttggtgag
    12841 aagtatttca ggatagatga gattcccttt gattcggaca gaaagtgcat gtcggtaatt
    12901 tgtgaaaaca aaaacggaga aatctttgtg tttaccaagg gtgccccgga cgtgattatt
    12961 aataaatgct ccaaaatcta tacttcaaag ggaatagtaa atttggatgg cgaagggaca
    13021 agaaaggttc taaaggctaa cgacagtatg gcagatgagg cgttaagggt attgggagtt
    13081 gcatacagga agctgggatc gagattttac aaaaacagtg aagttgaaaa ggatcttata
    13141 tttgtcggtc ttatcggaat gatagatccc ccaagaaaag aagtgttgga ggctgtgcga
    13201 aaatgcaggc ttgcagggat aaaaccggtg atgattaccg gagaccacaa aataactgca
    13261 gcagccatag caagagagct gaatatcgca acggcgggtg acagagtggt taccggggcg
    13321 gaactggaac aaatggatga caataagctt caggaagtag tggaggaagt gtcggtttat
    13381 gccagggttt ctccaagaca taagctaagg atagtgagag cactaaagaa gagaggacac
    13441 attgttgcaa tgaccgggga cggagtaaat gatgcccctg caattaagga agccgatatt
    13501 ggagtatcca tggggataac cggaactgat gttacaaaag agtcggcggc aatgattctc
    13561 agggatgata attttgccac cattgttgcg gccattgagg aaggcagggt tatttacagt
    13621 aacatacgta aatttatcag atatttgctt tcctgtaaca tcggcgaggt tttcaccatg
    13681 tttgtcggta tgctgatagg attcccgcta cccctgttgc cgattcaggt tttatgggtt
    13741 aatcttgtca cggatgggct tcctgcaata gccctggggt ttgagccggc agaaaaggat
    13801 attatgatga ggcctccaag gggggcaaag gaaaatatat tttcacaagg tcttctgaat
    13861 cttatactgt ttagaggaac tattatcgga gtctgtacac tggcggtatt tgcaagcata
    13921 tttcatttta catccagtgt tgaaacggca aggacagctg cctttgtaac tttggttatg
    13981 acacagctta tgcatgtttt tgaatgcaaa tccgaaagaa aaaacatatt tgaggttccg
    14041 tttttcaata acattgccct ggtgttggct accatgtgct ctttaaccat gattctttcg
    14101 gcggtataca taccttttat gcaggaaata tttaaaacag tgcctttgtc agtaaatgac
    14161 tggatattaa tcgccggatt ttcagcggtt ggccctgtgg tttcaagctt cttcagggtg
    14221 aatgcacgtt atattagggg gcatatttac aagtaaaaaa acggaagaaa catgagtctt
    14281 tgaaatgttt gccgcatatt ttataataaa aagtaatttt aacctttttg taaccttttt
    14341 ttttatgagt atatatataa tataattgaa gtacttcaaa gaaagaaata cataaactta
    14401 ttataactct tgtatacaag ataagctagt agagcatatg ctgttaaaat gcatacggaa
    14461 ttactttggt agtgattatt taataacgga tttgattgat tttgaaaatt gcatatatat
    14521 tatttgttct accggatgaa gaaacctatt ttgcttagtc attgcactgt tttaagttca
    14581 ttttgatcct tgttggcggc ttacatcatt cttacatgtt ctattggtca cgatgcgatg
    14641 ttataactgt tctttataaa ctttatttac tacttggcac atttgtactc atatagcttg
    14701 ctattagtgg attaataagt taacgtgcac gtgtgacaat atataaatga gagtattttt
    14761 gctttgaagt ttgggtttag aatttagggg gaaaattaga aagtcataaa gtcaaatgta
    14821 ggtggtgtaa taaccgcggt tagagagata agtgtaaaaa acactaatat actgatatgg
    14881 agagcaagtt tgcaaagaaa aaatcaatct acctgttttg tgggaactta ttggggcgga
    14941 aatacaatta aattggcatg aagcaggtag gaaagacttc taaagttaca gaaattaatt
    15001 ccacataact aaaacatgat taaaaaacaa tatagtattt gggtgatcac cccgggaagt
    15061 ttgtttataa caaacggttg aacattgaaa aggctgatac catactatta tgaaataggt
    15121 ctagtgcatg ggctggagta cggtcctgat taatgccagc ccgcgattgt tttgtagtac
    15181 cgtactctta caattttcac ggattagtgt cacgtaacag tattgtgtta taaagtacta
    15241 attcctcagg ctgttataga ttatatagcc ccccaaagcg aaaaacgcca gaggaattaa
    15301 caatccaaaa ttaatagacg atgttactat attaagaatt ataatggaaa gagagacaac
    15361 ggatacagtt gtcaaaacag aaatcgggat taaaagacct ttgggttttc ccgagaagag
    15421 atagagctga aacaatccaa aggcaacaga aataatataa attggccatg tatatttgga
    15481 aaaataccat ccggtaaacg attcgaacag aaacagaaaa cctgtaacag tcaatatacc
    15541 accgggcaca agaagacctg gggatttctt accgacaaag tagctgaatt caaaaatcaa
    15601 acctaatacc aatataatca ttggccacag acgagaaata ctgaatatgt tgacatcaaa
    15661 aatgctgtca cataacaata gtcctccgat aaatataaaa actagtcccc atgcggggct
    15721 tccttttttc atatgaattc ctccataatt aattatttaa gtttttttct ttttatttta
    15781 ttatacgtgt attagagaag aaagtcttat gtaaaaataa aataatttgg gtattgtaat
    15841 ctgaattatt gtatcgccgg gatgggtgcc gaaaatatag gactctattt tttaatcgta
    15901 ttaatgtata atatatataa aatgtgtttt tggctatatt aaattaggct tcatcaaata
    15961 taaaaaacag gcttaaataa ttatattggt tgaaaaggaa tgatttttat gggggcaaaa
    16021 gtattaactg tggaagatga caatagtata agggggtttc ttgtcacaat tttaaaaaga
    16081 aacaatttta ttgtatctga agctgttaca gggaaagagg ctttaagaaa ggtagaactg
    16141 gaaaaacccg aagttgtttt gctggatatc atgcttccgg atattgacgg atttgaggtg
    16201 tgtaaaaaga tatcgcagca gcatcccgat attccgataa tcatgctgac agcaagggga
    16261 gaagacacag acaagataaa cggtctggag ttgggtgcag acgattatat tataaagcct
    16321 ttcaacccaa tggaggttat tgcaagaata aaagccataa tcaggaggac cagtaagact
    16381 gatttttcaa atgaaataga gattggaccg tttaaaattg acttgaattg tcaaggagtt
    16441 tataaaaatg gacaggtttt ggatctgaca cctacagaat tctgtctgtt gaaagttttt
    16501 gcgaacaacg tcaataaatc tttaaccaga gatgatattt taaactgggt ttggggagaa
    16561 aactatgtcg gagatacaaa aactgttgat gtgcatgtaa gaagacttcg agaaaaaata
    16621 gaggatgatc catcaaagcc gaagtatata gaaacggttt ggggacatgg gtatatgtgg
    16681 aggaaaggag attaatgtga ggggagtcag aggcagaatt gcaggcactt atttgattat
    16741 aactctttta gcggttctga tttttgaagc gatattgatt tatatacttg ttcggtttta
    16801 ctatacaaat attgagtcgg aactaaatgc ttctgctaat acaatagttc ataattatga
    16861 agtatatttt aacggtgatg atcttgaccg ggatgcaaaa ctattgatgg agtccgtatc
    16921 atcaaatact gtagcccaga ttcagattat cgacagagaa aaggtagtac tggttgattc
    16981 tataaatcct gtttccgtag gagagagcct tgactatccc gatatcaacg gtgcactaaa
    17041 aggcaaaaag actacatgga gagggagaaa tccgcagaca aatgagtcca tcctttcagt
    17101 atccagtcca ataaaaaaga acgaagaagt aatcggagtt gtgagggtta tcactgctct
    17161 tacagatgtt aacaaaatac tatttaacca tgtgttaact ctgattgttt taggtacctt
    17221 tattgtaatt ctgatttttg tggcaggagt gtttctgtcc aataccatca taaaacctgt
    17281 taaggaaata acctgcgcgg cagaagcaat ggcacggggc agatttgacg ttcgggtcaa
    17341 caaaagatac gatgatgagg tgggtaaact ggcggacact ttgaattaca tggccgaagc
    17401 agtgtccaag catgagaaaa tgaagaatga ttttattgca tcaatttccc atgagattcg
    17461 cacgcctctg acatcaataa tcgggtgggt tatcgccatt aattcaggaa acgtggaaaa
    17521 cagtgaggaa ataagagaag gccttggcat tgttgaaagg gagagcgagc ggctggccgc
    17581 aatggtcgaa gaattgctgg atttttcaaa atttgatgca gggtctataa accttaaaat
    17641 tgatcttgtg gacttaaaag aattaatttc gcatataaca aagcagatgg agccacgagc
    17701 caagagagag ggcatcttta ttgtaatgag aacagatgaa gatttaccct ttatagatgc
    17761 agatgaaaac aggctgaaac aagtattaat aaatattatt gataattcgt ttaaattcac
    17821 tcagaaaggt gggtatataa atatcatagc aaagcagttg aaggacaagg tcttaatatg
    17881 tgtagaagat aacggttgcg gtatacctga agacgacatt tccaaagtaa ctcagaggtt
    17941 ttttaaggga aatagcaatg cagccggcag cggtttggga ctagctatct gtgatgagat
    18001 tataaaactt cataatggtg aaatgaacat tgaaagcgtt gtggaaaaag gaacggcagt
    18061 aaatataatt ttaccggtga gagcataaac tttaactttt ttgtaatcct ttttattatt
    18121 tatatatata gaattgttat tggaagagga tgaatatgaa tacgatgaac acgataatta
    18181 aacgaaacag aataattctc aaatatacat taactgcaat tgttgtttca ctcttattaa
    18241 caattactgg gtgcgatgta ttacc
//
LOCUS       DAOOJC010000003        15380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_3714_length_15380_cov_4.038891, whole genome shotgun sequence.
ACCESSION   DAOOJC010000003 DAOOJC010000000
VERSION     DAOOJC010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 15380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15380
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3714_length_15380_cov_4.038891"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..893)
                     /gene="fliP"
                     /locus_tag="PLA01_00145"
     CDS             complement(111..893)
                     /gene="fliP"
                     /locus_tag="PLA01_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517978.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HPC99741.1"
                     /translation="MKTRLKYITVIISIITVLILLASNVYAEPDTISLPKLGFTVESS
                     DNPQDVSTSVQILLLLTVLALAPSILIMMTSFTRIIIVLSFLRNAMGTQQMPPNQVLI
                     GLALFLTLFIMNPVLTEINDTALKPYSNQEISQDVAIDRASATIKRFMILQTSKEDLG
                     LFVSLSGMKTPIKEEEIPNLPLTIIIPAFLISELTIAFKIGFLIYIPFLVIDMVVSST
                     LMSMGMMMLPPVMISLPFKILLFIMVGGWNLITQSLVNSFVL"
     gene            complement(877..1407)
                     /locus_tag="PLA01_00150"
     CDS             complement(877..1407)
                     /locus_tag="PLA01_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HPC99742.1"
                     /translation="MESLISAVLFIIVFGSVLFVAYVTTRFVGSKTNKIMKGKYINIV
                     ETVNLGFDCKLHLVKVGSEFVLISTSGKTTRLLTRVRLEEYSNEKSIASTNTFNFKNV
                     FEKYFQGFKHIQEFQGFRPIQGIKGFFAARDNESAVTQKDQTFNKNLERLKDISTGMS
                     LHKTEYGEEYTNENQA"
     gene            complement(1459..1821)
                     /locus_tag="PLA01_00155"
     CDS             complement(1459..1821)
                     /locus_tag="PLA01_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC99743.1"
                     /translation="MGKRILIVDDAAFMRMMIKDILTKNGYEVAGEAENGARAIDKYK
                     ELTPDLVIMDITMPELDGIEAVREIKKINSESKIIMCSAMGQQAMVIESIQAGARDFI
                     VKPFQAERVVEAVKKVLG"
     gene            complement(1906..3093)
                     /gene="fliY"
                     /locus_tag="PLA01_00160"
     CDS             complement(1906..3093)
                     /gene="fliY"
                     /locus_tag="PLA01_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HPC99744.1"
                     /translation="MGDMLSQEEIDALLNGDKTDEGENINSDSELNTNINSDSELDTQ
                     QIDALGEIGNISIGTSATTLNSLLGQKVTITTPRVMVCTWKELLDKYYELNVAVKVEY
                     TDGLNGLNLLLLKESDVKIIADLMMGGDGSNNEGELTELHLSAISEAMNQMIGSAATS
                     MSSMFDKRIDISPPKAFSLTIDSISPENSFDDESRVVQIAFKMQVGELIDSEMMQIIP
                     VQFAKELVDNILNAESRQNNDHSSPVSVEPPPVQQQMPISNNHAGVQPSPQQNPYQPY
                     GQQYVQPEPQAHNKPKTPINVQPAQFESLEDGMISIEKKNIGLIMDVPLQVTVELGRT
                     NRLIKDILEFGPGSIIELDKLAGDPVDILVNGKVIAKGEVVVIDESFGVRITDIIDPS
                     KRL"
     gene            complement(3093..4082)
                     /gene="fliM"
                     /locus_tag="PLA01_00165"
     CDS             complement(3093..4082)
                     /gene="fliM"
                     /locus_tag="PLA01_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HPC99745.1"
                     /translation="MGDILSQNEIDELLKALNTGEIDVQQMNSKTEEKKVKIHDFRRP
                     SKFGKDHIRTLNVIYDNYARLVTNFLSGYLRALVQVDVISVEPIAYYEFSNSLSNPAV
                     LAVVELAPLTGSVILELAPSVAFALIDRILGGKGLPIEKIREFTEVELAIVERIIIQI
                     LNLMKEPWENVIEVKPRLEKIETNAQFAQIVSANEMIALVTLGTRIGDVEGMVNICIP
                     YMAVEPILSKLSTKYWFASAEKEKSPEDKRTIEHKIESTKVPLSAILGRTTIQVAELI
                     EMQCGDVIPLNTNVNGKLEILVGDILKFYGSPGVKKNKVSVKITDVIRREDMQ"
     gene            complement(4095..4595)
                     /locus_tag="PLA01_00170"
     CDS             complement(4095..4595)
                     /locus_tag="PLA01_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HPC99746.1"
                     /translation="MEGKSGYFILIGIVAFLSLSLAVLIIILFLQGGFVNSASDNPKI
                     VESPSDDELVKLKLFEEKTPFNLKNENSGKNPIIVISAEISYFKEVRDIQDTTAKIEA
                     NKSMLQEIVGTYFQSLTLKDVMKDDAKEKARAELKKRMNERLLSNEKARDDIVYTIVF
                     DQWFYQ"
     gene            complement(4689..4889)
                     /locus_tag="PLA01_00175"
     CDS             complement(4689..4889)
                     /locus_tag="PLA01_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HPC99747.1"
                     /translation="MIKLTKLNGTEFVLNCELIEIIEKTPDTIISTTNGKKFVVVETI
                     EEIVNKVLQYKRSIFLFNSNNQ"
     gene            complement(5069..6418)
                     /locus_tag="PLA01_00180"
     CDS             complement(5069..6418)
                     /locus_tag="PLA01_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HPC99748.1"
                     /translation="MMRSMFSGVSGLRAHQTRMDVIGNNIANVNTIGFKSGRVTFEEV
                     FSQTLQGAGAPDSTTGRGGTNPMQVGLGIDVGTIDTITTRGSLQRTDNPTDLSIEGDG
                     FFIVKGGSSGTYMFTRAGNFGIDRLGNLVTGSGLNVYGWQTYTRKADGSYEFDTESPV
                     EPINLFSDAVNKNKKMLAARATTSAILTGNLDASTPVYAGGSAVGSGASGTTDSFQFT
                     VPLTVYDSLGNDYKLSVNFRKVSAVGGSGGTTTWEWQVAAASGINLATASGSIQFNTK
                     GQVVSGPELTPSIVVTPASSIGTEDINVMLDFSNLTMYAADSSVKPTNVNGYPPGELV
                     TFNIGSDGMIVGVYSNGQQQPLGLVALAGFQNPAGLQKVGDNLYITTSNSGEFTRGVK
                     AGSDGTGTLNPGTLEMSNVDLSKEFTDMIVTQRGFQANSRIITTSDEMLQELVNLKR"
     gene            complement(6573..6962)
                     /locus_tag="PLA01_00185"
     CDS             complement(6573..6962)
                     /locus_tag="PLA01_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HPC99749.1"
                     /translation="MIVNNNFYANVQKVVNRQEAPTINKPDNAGKGSFDEILKSKINE
                     SSEIKFSKHAQMRLQMRNINLSEAQKERISKAVQKAGEKGVKESLVLVDDMAFVVDVR
                     SKTVITAVNSNELKENVFTNIDGAVFT"
     gene            complement(6959..7813)
                     /locus_tag="PLA01_00190"
     CDS             complement(6959..7813)
                     /locus_tag="PLA01_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HPC99750.1"
                     /translation="MAVDGISRQKTIEEIVKSTENKSGQRKVGELGKDEFLNLLVTQL
                     RYQDPLNPVEDKEFIAQMAQFSALEQMQNLNTSFSATKAFGMIGKYITATMVNESTFE
                     TETIEGMVDSVKMEGSKTYVVVGGRDVPVEKVIGVSEDSGVFNNSNISQYTGIIGYEA
                     KGCVYNPNTGEIIGVNGVVREIEKGRYENYAVMDGVKVNISTAATGTSSKDPEFWNTY
                     LTESKDHEISLIITDELGNGTKVPVTGVLRDFTIAPDGKITGVLDEVYVPVESIYNIK
                     EQVREVEE"
     gene            complement(7829..9370)
                     /locus_tag="PLA01_00195"
     CDS             complement(7829..9370)
                     /locus_tag="PLA01_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HPC99751.1"
                     /translation="MINSNTVLPLVSTIVGQQGNSQTKRDSSDFNFADFKDILSSSVN
                     RTYSKQKSERAYTAAKSEKPIVGKEVPGQVKSFNEAITKSQRHFGNNKAAGKEETGTE
                     YDKNKIEDHSEDGGLKEKVLKEKVLKETLAMTLGITGNELDTLMAALNINPCEIMDSA
                     NIEAIAQRISDYIGLSSDQQRTLSKILPLVQKEIKHSFDELQIFSNLVQKPEMTTDKE
                     DWIELEGVDIEVIEEAGEPGDKIVQALNRLKEMVHQQPDKLISQISGKVQNVLKQSIE
                     PVDTASIQIAVKEENLPVEDESENSSDESEDSDLKDRKDSIETMDLKDNQGFVSKIKD
                     DGGNEFVALSFNVNQKPVQEAGISQTIQSKTPVTRIEIFTQVVEKAKVLLSGDKSEMV
                     IDLKPDHLGKLALKIATERGIVVAKFIAENEQVKAALESNMNLLKESLEKQGFSVQEF
                     SVFVGGNRERGGNGENMQHGSHVKSGVKDKLPAIGMVDVESLEQMNSGNSYYDDGTSR
                     INLTA"
     gene            complement(9360..10217)
                     /locus_tag="PLA01_00200"
     CDS             complement(9360..10217)
                     /locus_tag="PLA01_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF615 domain-containing protein"
                     /protein_id="HPC99752.1"
                     /translation="MTKENESKISPEIAGSITARKAKKKNGILFGLLILLASLVVIAG
                     VVTSVFWIIIRSNANGFADKYRNQIKGIPVLRHALPAPPEDYDPLAPENLSGKELENK
                     YKEFRRKNSELSSELEKTKKELDELKKIQLSKDDFMERIDELTEEKEKLKKDMEAENT
                     RLKEEISKIQDLLAKEDKEGFREFFEKMDKDTAQSLYAKILGEQKEDEQAKQFAQIYE
                     KMDAGDAAAIFEELGSSKIELVVNVLKSMKRDAAAQIIAEMDEKFAAQVTDILSKEFG
                     LKGGENIDQ"
     gene            complement(10241..10702)
                     /gene="fliJ"
                     /locus_tag="PLA01_00205"
     CDS             complement(10241..10702)
                     /gene="fliJ"
                     /locus_tag="PLA01_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HPC99753.1"
                     /translation="MTVGKFVFRMQSLLDLKTQMEQSIKNELGRAIQEMERQKRILNG
                     LIEEREVYIFNVNQMYEKSVTVAKLREYSTYISYLNDRVRVQQDNVIIARNNVDKYRE
                     KLIKAMQEKKMFDKLKEKKHEEYMKEQLKEEQKLNDEIVSFNIVHDGLGEY"
     gene            complement(10788..12101)
                     /gene="fliI"
                     /locus_tag="PLA01_00210"
     CDS             complement(10788..12101)
                     /gene="fliI"
                     /locus_tag="PLA01_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HPC99754.1"
                     /translation="MAVVNLTKYQDILEKTNLIEYIGKVTKVVGLTIESNGPEVNIGE
                     ICRINALRENKKIAAEVVGFKDNKVLLMPLGNMSGIGPGSKVVASGEYLSIGVGSDLM
                     GRVIDGMGNPIDGKGEITYECTYPVDNAPPEPLERNRIKDPLPLGVKCIDGLLTVGKG
                     QRVGIFAGSGVGKSTLIGMIARNTKADINVIALIGERGREVREFIEKDLKEEGLKRSV
                     VVVATSDQPALVRLKGALLATSIAEYFRDRGKDVLLLMDSLTRFAMAQREVGLAIGEP
                     PVSRGYTPSVFAVLPKLLERSGNSGSGSITGLYTVLVDGDDLTEPVTDTARGILDGHI
                     VLSRALANRNQYPAVDVLASVSRVMPDVVTKEHMDIAKEIKKAMAIYRDAEDLINVGA
                     YVKGSNEKIDYAISVIDRIQSFIEQGVSDKYTFDETLSILKDILV"
     gene            complement(12135..12890)
                     /locus_tag="PLA01_00215"
     CDS             complement(12135..12890)
                     /locus_tag="PLA01_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HPC99755.1"
                     /translation="MYNRIFKSNQINMGIPVQIRVPLNYQNIKRTNAVHIGPDFETVK
                     NKIEDAQEVLEKTRLEAESIIKEAELEADRIIENARKKAEELKSLIEEEARQKGYQDG
                     YEEAKKQYDDLLREAEKVKENALKEYKEVLEGIEKDAVDVILGVSRKVIDDEISLNQG
                     YLLNLVRQAFEKCSNRENIALKVSSKDYEFIEENKDMILSMTEGLDGLEIIEDKSLKP
                     GNLILETAYGSVDSGVNTKIKKIEESFEKVLNK"
     gene            complement(12883..13905)
                     /gene="fliG"
                     /locus_tag="PLA01_00220"
     CDS             complement(12883..13905)
                     /gene="fliG"
                     /locus_tag="PLA01_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HPC99756.1"
                     /translation="MARVASAKMELSGKEKAAMLLIALGPEKSASIFKHLKEEEIEQL
                     TLEIANIRAVTPEEKEKVLEEFYQICLAQDYIAEGGINYAKEILEKALGTQKALDVLN
                     KLTVSLQVKPFDFVRKTDPSQLLNFIQNEHPQTIALILAYLKPQQSSVVLSALPQEKQ
                     ADVARRIATMDRTTPEVIKEVERVLEKKLSSLVTGDFTTAGGVQAIVDILNSVDRGTE
                     KYIMETLEIEDTDLAEEIKKRMFVFEDILTMDNRFIQRFLREVDNNQLAIALKGTSEE
                     VQSKIFSNMSKRLVEMMKEDMEYMGPVRVKDVDEAQQKIVNIIRKLEETGEIVISRGG
                     GDEIIV"
     gene            complement(13925..>15380)
                     /locus_tag="PLA01_00225"
     CDS             complement(13925..>15380)
                     /locus_tag="PLA01_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HPC99757.1"
                     /translation="TLTIGIIMLTRTTYVPLMTIEDSENLSEIEKTLQEKKINYKPGD
                     KGQILVDSKEKNKAEFALASAGLTEKGMTFEDAWSLINVSTTESDKKQLWHNFKKNSL
                     IAKLKMFDNIKDADVELTIPDDTMFFIENEEEAKASVRIKPKGEITPEQVEGIVRVVA
                     SSIEGLDPKNVTVVDNNFNILNQDLSDGMSIPSSQYKLKLRIKEELEKNIKKLYSGRS
                     DSYDFISVAVNPVLDLDRVTTNRKEIEKPTGLDEAVASQEKHKEELENGYGGEAPGMD
                     SNPGTGQVPGYPIDGGNNSSYNKNSEIINRIFTETMTAQEKAIGTVNFDESSMTVALW
                     YGDRVPDDSRLTQEFIDQFKQSISTATGIPVSRISVDKQKLAPAEEDVVSAWERIKEF
                     LDNYGFFILMILLIVALMIAVIPRKGNQQSELEPQLAAANGAIAIDNEIRDTVPEITF
                     EERSEIKKQIEKFVREKPESVAQLLRNWLSEDWE"
BASE COUNT         3927 a         3782 c         2439 g         5232 t
ORIGIN      
        1 acatcggtgc tgcaacatat attactgtca gaatagccct ctgtgccaat tctattacca
       61 ttccctgttc catatccctg cacccccgaa ttcccatatt tttttcttat ctataagaca
      121 aaactgttaa ccagtgactg agtaataaga ttccatcccc ctaccattat aaacagcaga
      181 atcttaaacg gcagtgaaat cattaccggt ggaagcatca tcatacccat ggacataagg
      241 gtgcttgaaa ccaccatatc aattaccaga aacggtatgt atataagaaa accgattttg
      301 aatgctattg tcaattcgct tatcagaaat gccggaataa ttattgttaa aggaagattc
      361 ggaatttctt cttccttgat gggagtcttc atacccgaca acgatacaaa aagtcccaga
      421 tcctcctttg atgtttgcag aatcataaac ctcttaattg tggcggaagc tctgtcaatt
      481 gcaacatctt gagagatctc ctgattcgaa tagggcttta gtgcagtatc gttaatttct
      541 gtcagcacgg ggttcattat gaacagcgtt aaaaacaaag ctagcccaat caacacttga
      601 ttcggcggca tctgctgagt ccccatcgca tttcttaaaa aggaaaggac aattattatt
      661 ctggtaaagc ttgtcatcat gattagtatt gacggagcca atgccagcac agtcaaaagc
      721 aacaaaatct gaacactggt ggaaacatcc tgtgggttgt ctgatgactc tacagtaaac
      781 cccagcttgg gtaacgatat agtatcaggt tcggcataca catttgatgc caatagaatt
      841 aatactgtta ttatacttat tattaccgta atatacttaa gcctggtttt cattagtgta
      901 ttcctctcca tattctgtct tgtgcaagct cattcctgtt gaaatatcct taagcctttc
      961 tagattttta ttaaaagttt ggtccttttg agtgactgca ctctcattat cccttgctgc
     1021 aaaaaaaccc tttattcctt gtattggtct aaaaccctga aattcttgta tatgcttaaa
     1081 accctgaaag tatttttcga agacattctt aaaattaaaa gtgttagtac tggctatcga
     1141 cttttcattg ctatattctt ccaatcggac ccttgtcaaa aggcgggtgg tttttcccga
     1201 tgttgatatt aaaacaaact ctgaccccac ctttaccaga tgcagcttac agtcaaaccc
     1261 caaatttacc gtttcaacaa tatttatata tttccctttc attattttgt tcgttttgct
     1321 ccctacaaac cttgtagtga catatgccac gaacagaacc gaaccgaaaa caattataaa
     1381 caaaacagca cttatcaggc tctccatttt atactccctc ttccgtaatc tcttagtaca
     1441 tccaaacctc taccgaaatt atcccaggac tttttttaca gcctcaacga ctctttcggc
     1501 ctgaaagggc tttacaataa aatcccttgc accggcttga atagactcta ttaccatagc
     1561 ctgctgtccc attgccgagc acatgattat ttttgattcc gagttgattt ttttaatctc
     1621 ccgtacagct tctataccgt ctaattcagg catagtgatg tccataatca ccaaatccgg
     1681 agtaagctcc ttatacttgt caattgcccg ggctccattc tccgcttctc cggccacttc
     1741 gtacccgttt ttggtaagaa tgtcctttat catcattctc ataaaagctg catcgtcaac
     1801 tataagaatc ctttttccca tcatttaccc tctccttaat tatttgtctc ttcccttgca
     1861 aaatcagtat taataatgtc tttatgggtt ttatatttaa tacagctaaa gtcttttaga
     1921 cggatcaatt atatccgtaa tcctgacgcc gaagctttca tctattacaa caacctcacc
     1981 tttggcaatt actttaccgt ttaccaaaat atcaacagga tcaccggcaa gcttgtccag
     2041 ctcaattatg gagccgggtc caaattccaa tatgtccttg ataagcctat tggttcgtcc
     2101 aagctcaacc gttacctgca aaggtacatc cataataagc ccgatattct ttttttcaat
     2161 tgatatcatg ccgtcctcaa ggctttcaaa ctgagccggt tgtacattta ttggcgtttt
     2221 cggcttatta tgtgcctgcg gttctggctg tacgtactgc tgcccgtacg gctgatatgg
     2281 attctgttga ggactgggtt gaaccccggc atgattattg gaaattggca tctgctgctg
     2341 aaccggagga ggctcaacgg acactggtga ggaatgatcg ttattttgtc ttgattccgc
     2401 attcagtata ttgtccacca gttcctttgc aaattgaaca ggtattatct gcatcatctc
     2461 actgtcgatt aattcaccaa cctgcatttt aaaggctatt tgaactactc tgctttcatc
     2521 atcaaaggaa ttctcgggac ttatactgtc aattgtcaga gaaaatgcct ttggaggaga
     2581 aatgtcaatc ctcttgtcaa acatggaaga cattgaagtc gctgccgacc caatcatctg
     2641 gttcattgct tcgctaattg cacttaagtg aagctctgtt aattccccct cattattgct
     2701 gccgtccccg cccatcatta aatcagctat tatcttaaca tccgactcct ttaataagag
     2761 aaggttcaaa ccgtttaagc catcggtata ctctacctta accgcaacat tcaattcata
     2821 gtacttatcc agcaattcct tccatgtgca taccataact ctgggagtag taatggttac
     2881 cttttgtcct aagagggaat ttaaggtagt tgcagaagta ccgatgctta tattgccaat
     2941 ttctcccaat gcgtcgattt gctgcgtatc tagttcggaa tcgctgttta tattcgtatt
     3001 tagttcggaa tcgctgttta tattctcacc ttcatctgtt ttatcaccgt tcaataaagc
     3061 atctatttct tcttgagaca acatatctcc cattattgca tatcctccct tcttattaca
     3121 tccgttattt taactgaaac cttatttttc tttactcccg gtgaaccata gaattttaat
     3181 atatctccca ccaaaatctc taatttccca ttgacatttg tatttaacgg tattacatct
     3241 ccacactgca tttcaattaa ttcggctacc tgaattgttg ttctgccaag tattgcactt
     3301 agaggtactt ttgtactctc aattttatgc tcaatagtcc ttttatcctc agggcttttc
     3361 tccttctcgg cacttgcaaa ccaatacttt gtacttaact tggataatat aggttctaca
     3421 gccatatatg gaatacagat attaaccatt ccctcaacat caccaatcct tgtccccaag
     3481 gttactaagg ctatcatttc atttgccgat actatttgtg caaactgtgc attggtctca
     3541 attttctcaa gccttggttt tacttcgatt acattttccc agggctcttt cattagattc
     3601 aggatctgaa ttattattct ttcaacaatc gcaagctcta cctcggtaaa ctccctgatc
     3661 ttttcaatcg gaagtccctt tccgccaagt attctgtcaa ttaaagcaaa agcaacggaa
     3721 ggtgccaatt ccaaaataac cgaaccggta aggggagcaa gttcaacaac tgcgagcaca
     3781 gcaggattgg ataacgaatt gctgaattca taatatgcta tcggctccac tgaaataaca
     3841 tctacctgaa ccaaggccct caaatatccc gaaaggaaat ttgtaacgag tctggcataa
     3901 ttgtcgtaaa taacattgag agtccttata tgatcctttc cgaatttgct tggtcttcta
     3961 aagtcatgta ttttaacctt cttctcttcg gttttggaat tcatttgctg gacgtctatc
     4021 tcaccagtat taagggcttt taatagctcg tctatttcat tttgagataa gatatctccc
     4081 attcgatgca ccccctactg atagaaccat tgatcaaaaa caatggtata gacaatatcg
     4141 tcccttgcct tttcatttga cagcaaacgt tcattcatcc tctttttaag ttctgccctc
     4201 gccttttcct tggcatcatc cttcattaca tctttgaggg ttaatgattg aaaatatgta
     4261 ccgacaattt cctgaagcat actcttgttt gcttctatct ttgcggtagt atcttgaata
     4321 tccctgactt ctttgaaata agatatttca gcactgataa ctattattgg attcttcccc
     4381 gaattttcat tcttcaggtt gaagggtgtc ttttcctcaa acagctttaa ctttaccagt
     4441 tcatcatcac tgggtgattc aactattttc ggattgtctg acgcagaatt cacaaaccca
     4501 ccctgtaaaa aaagtattat tattagtacc gcaagggata aggacagaaa tgccacaata
     4561 ccaataagaa taaaataacc gctttttcct tccaaaatct atgccccctt tatcctttaa
     4621 gtagataaca taaaatggcc gagctgttat aacaaaatgc acaatagtat taagagcata
     4681 aagtccaatt actggttatt actgttgaat aaaaagattg atcttttata ctgtaaaact
     4741 ttattgacta tctcttcaat agtttcgaca acaacaaatt tcttgccatt tgttgttgaa
     4801 ataatagtat caggtgtttt ttcaatgatt tcaattaatt cacaattaag aacaaattct
     4861 gttccattta acttagttaa tttaatcata atatttttac ctcaaaaact caatagaaaa
     4921 attatatttt tgtgaaaaaa ttatgttttt gtgtacttat tttacggtct gggtatactg
     4981 ttagttgcta cattagtaat aaattctatt aattctcaat ctgtcaggtg ggatatgccc
     5041 cacccgacag aagagttact caatttaact atctcttcag attaaccagt tcctgaagca
     5101 tttcgtcaga tgtcgtaatt attctgctgt ttgcctggaa ccctctctgg gttacaatca
     5161 tgtctgtaaa ttcttttgaa agatcaacat tcgacatttc aagagttccg ggattcaacg
     5221 ttcctgtgcc gtctgaaccg gctttaacac cccgtgtaaa ttctccggag ttgctggtgg
     5281 tgatatacaa attatctccc accttttgca gtcctgccgg gttttgaaat ccagccagtg
     5341 caacaagtcc caacggctgt tgctgaccgt tgctgtatac cccaactatc atcccgtcgg
     5401 atcctatatt aaaggtaaca agctctcccg gcgggtaacc attcacattt gtaggcttta
     5461 cagaactgtc tgctgcgtac atagtgagat ttgaaaaatc aagcattaca tttatatcct
     5521 cggttccaat acttgatgcg ggagtaacaa caatgctcgg tgtcaattcc ggtcctgaaa
     5581 ccacctgtcc ttttgtatta aactgaatac ttccggaggc agttgctaag tttattcccg
     5641 atgcagcagc cacctgccat tcccatgtag tagttcctcc ggaaccaccc acagcactga
     5701 cttttctgaa gttcacactc aatttgtagt catttcccaa tgagtcgtat actgtaagcg
     5761 gtactgtgaa ttgaaaactg tctgttgttc ctgaagcacc cgagcccact gccgatcctc
     5821 ctgcgtagac aggcgttgaa gcatccagat tgcctgtcag tattgcactg gtagttgctc
     5881 tggctgccag catttttttg tttttattga cagcatccga gaaaaggtta atcggctcca
     5941 cagggctctc tgtatcaaac tcataagaac cgtcagcttt ccttgtatag gtctgccagc
     6001 catatacatt caaaccgctt ccggttacaa ggttccccag cctgtctatc ccaaaattac
     6061 cggcccttgt aaacatatat gtacccgagc ttccaccctt tacaataaag aaaccatcgc
     6121 cttcaatgga taaatccgta ggattatcgg ttctctgaag acttccgcgg gtagttatcg
     6181 tatcgattgt tcccacatct atccccagtc ctacctgcat ggggtttgta ccgccacggc
     6241 ctgttgttga atcaggagca cctgcacctt gaagcgtctg gctgaaaacc tcctcaaagg
     6301 ttacccttcc tgatttaaag cctattgtat ttacgtttgc tatgttgttt cctataacgt
     6361 ccattcttgt ctggtgtgcc cgaagtccgg aaactccgga aaacatagat ctcatcatac
     6421 ttaaatgacc tccttcagct acgctataat aactaacaac gtcattgtct attccttctg
     6481 tcagtcggga ataggtagcc tcccgaaagg gtccggctac aatatatatc tgcgatttca
     6541 cgggatgcta agctaaaaac cttgatacaa tgttatgtaa atacagctcc atcaatgttt
     6601 gtaaaaacat tttccttaag ctcattgctg ttaaccgcag tgattaccgt cttgctcctt
     6661 acatctacta caaatgccat atcgtccaca agaaccagtg actccttaac ccctttttcc
     6721 cctgcctttt ggacagcctt tgaaatcctc tccttctggg cttccgaaag attaatattt
     6781 ctcatttgaa gcctcatctg ggcgtgtttt gaaaacttaa tctcactgct ttcatttatc
     6841 ttactcttta agatttcatc aaagcttccc tttccggcat tatcaggctt gttgattgtg
     6901 ggagcttcct gcctgttgac aaccttttgg acatttgcat agaaattatt atttactatc
     6961 attcttccac ctcacgtacc tgttccttaa tgttgtagat actctctacc ggaacataca
     7021 cttcgtctaa aaccccggtt atttttccgt caggtgctat ggtaaagtct ctcaaaactc
     7081 cggtcaccgg aactttggtt ccgtttccga gttcatcggt tattatcagg gaaatctcat
     7141 gatccttgct ttcggtcagg tatgtattcc aaaactcagg atcttttgaa gaagtaccgg
     7201 tggcagcagt agatatattt actttgacac catccattac agcgtaattt tcatacctgc
     7261 ctttttcaat ttcccttaca actccattta ctccaataat ttcaccggta ttaggattat
     7321 atacacagcc tttagcttca taaccgatga ttcctgtata ctgggatata tttgaattgt
     7381 taaatacacc cgaatcctct gaaacgccta tgaccttttc taccggaaca tctctgcctc
     7441 cgacaaccac ataggtttta ctgccttcca tcttaacact atcaaccata ccctcaatgg
     7501 tctctgtctc aaaagtactt tcattgacca tggttgcagt tatatactta cctatcatgc
     7561 caaaggcctt tgtcgccgaa aagctggtgt taagattctg catctgctct aaggcactaa
     7621 actgagccat ctgagctata aattccttat cctctaccgg gtttaaaggg tcctgatacc
     7681 gaagctgcgt taccagaagg tttaaaaact catccttacc cagttctcca acctttctct
     7741 gtcctgactt attctctgtg ctcttgacaa tttcctcaat ggtcttttgc cggctaatac
     7801 catccacagc cattagaact cctccttgct atgccgttag gttaattctg ctggtcccat
     7861 catcataata actatttcct gagttcattt gctccaggct ttcaacatcg accatgccta
     7921 tagccggaag tttatccttt acgccactct taacgtgact gccatgctgc atgttttcgc
     7981 cattaccccc gcgttccctg ttgccgccta caaaaacact gaactcctga accgaaaagc
     8041 cctgcttttc caaagactcc ttcagcaaat tcatgtttga ctccaaagca gccttaacct
     8101 gttcattttc tgcaataaat ttagcaacta caattcctct ttccgtagca atctttaatg
     8161 ccagcttacc caagtgatca ggctttaaat cgataaccat ttcgctctta tcgccactta
     8221 gaagcacctt ggccttttca actacttgtg taaaaatttc tattcttgta acaggtgtct
     8281 tgctctgtat cgtctgtgat atccctgctt cctgaaccgg tttttggttt acattgaaag
     8341 acaaggctac gaactcgttg ccaccatcat ccttaatttt cgatacaaag ccctggttgt
     8401 cttttaagtc catagtctca atgctgtcct ttcggtcctt taaatcagaa tcctcggatt
     8461 catcggaaga attttccgac tcatcctcta ccggcaaatt ttcttccttt acagcaattt
     8521 gtatagaagc agtatctacc ggttctatac tttgtttaag aacattctga actttaccgg
     8581 aaatctggct tatcaatttg tcgggctgtt gatggaccat ttctttgagt ctgttcaatg
     8641 cctggactat cttatcgcct ggttctccgg cctcctcaat cacttcaatg tcgactcctt
     8701 ccaattcaat ccaatcttct ttatccgtag tcatttcagg tttttgaaca agattggaaa
     8761 aaatctgaag ttcatcaaaa gagtgtttaa tctccttctg caccagaggc aaaattttag
     8821 acaaggtcct ttgctgatcc gaactcagtc cgatgtagtc agaaattctt tgtgctattg
     8881 cttcgatatt ggcactgtcc attatctcac agggattgat gttcaatgca gccataaggg
     8941 tatcaagttc attcccggtt attccaagag tcattgctaa agtctctttc agcaccttct
     9001 cttttagaac cttctctttc agcccgccgt cttcgctgtg gtcttcaatc ttatttttgt
     9061 catattccgt gccggtctct tctttgcccg cagccttgtt attaccaaag tgccgttgac
     9121 tttttgttat ggcctcatta aagctcttta cctgaccggg tacttctttc ccgactatcg
     9181 gtttctcact ttttgccgca gtataagccc tttcgctttt ctgcttggaa tatgtcctat
     9241 taacggaaga gcttaaaatg tccttaaaat ccgcaaaatt aaagtcactg ctgtctctct
     9301 ttgtttgaga attcccctgc tgtcccacta tagtcgaaac aagcggtaaa acagtgttgc
     9361 tattgatcaa tgttttcacc tcctttcaat ccgaattcct ttgaaagtat atcagtaacc
     9421 tgagctgcaa acttttcatc catttcagca ataatctgag ccgctgcatc ccttttcatg
     9481 ctctttagta cattaaccac caactctatc ttcgaactgc caagctcttc aaaaatcgca
     9541 gcagcatcac ctgcatccat cttttcatat atctgtgcaa actgctttgc ctgttcatct
     9601 tccttctgtt ccccgagtat ctttgcatac aagctttgag cagtatcttt gtccatcttt
     9661 tcaaaaaatt ctctaaatcc ttccttgtct tcttttgcca atagatcttg tattttgcta
     9721 atttcttctt tcagtctggt attttccgct tccatgtcct tcttcagttt ttccttttcc
     9781 tctgtcaatt catctattct ttccataaaa tcatctttac ttagttgaat ctttttcaat
     9841 tcatccaatt ctttttttgt tttctccagt tcggatgaca actcagaatt ttttctcctg
     9901 aactccttat acttattctc cagttcttta ccgctgaggt tttccggagc aaggggatca
     9961 taatcttccg gcggtgcagg taaagcatgc cttagcacag gtattccctt tatctgattc
    10021 ctgtacttat cggcgaatcc attggcattg cttcgaataa taatccagaa aacacttgta
    10081 acaacaccgg ctataaccac cagtgatgct aacagaataa gcaatccgaa caaaataccg
    10141 ttcttttttt tcgccttccg tgctgttatg cttcccgcaa tttcggggct tattttgctt
    10201 tcgttttctt ttgtcatttc ttatctcccg aatagattat ttaatactct ccaagtccgt
    10261 catgaactat attaaagctt acaatttcat cattaagttt ctgttcttcc tttaactgtt
    10321 ctttcatata ctcttcatgc tttttttcct tcagcttatc gaacattttt ttctcctgca
    10381 tggccttaat cagcttttct ctatatttat cgacattatt ccgtgctatt attacattgt
    10441 cttgctgcac ccttactctg tcattcaaat aggaaatata tgtactgtat tcccgaagct
    10501 tagccacggt tacactcttt tcgtacattt gatttacatt gaaaatatat acttctcttt
    10561 cctcgataag cccatttagt attctttttt gtctctccat ctcctgaatt gctctgccca
    10621 gttcattctt gatactttgc tccatctgtg tctttaaatc taacaaggat tgcatcctga
    10681 aaacaaattt ccccacagtc atcacccgtc ctgttcatat cggctggcac agtgttttta
    10741 ccgttacatt tttacggtct ttgggatttt ataaaataaa ccgtatatca aaccagaatg
    10801 tctttgagta tgcttaacgt ctcatcaaaa gtatacttgt cggaaacccc ctgctcgatg
    10861 aatgactgta ttctgtcaat aacactgatg gcataatcta ttttttcatt gcttcccttt
    10921 acataggcac ctacgtttat aagatcctcc gcatcccggt atattgccat agccttctta
    10981 atctcttttg caatatccat gtgctctttt gttaccacat cgggcataac cctgctcacg
    11041 cttgccagaa catcaactgc cggatactgg tttctgttgg ccaacgccct ggacagtacg
    11101 atatgtccat ccagtattcc ccttgcagta tcggttactg gttcggtaag gtcatcacca
    11161 tcaaccagca cggtatacag ccctgtaatt gaacctgaac cggaattgcc cgatctctcc
    11221 aatagcttcg gtaaaacagc aaagaccgaa ggtgtatatc ctcttgaaac cggaggctct
    11281 ccgattgcca gtcccacttc tctctgagcc atggcaaacc tggtaagcga atccatcaaa
    11341 agaagcacat ccttaccccg gtctctgaag tactctgcta tggaagtagc cagcaatgca
    11401 cccttaagcc ttacaagtgc gggttggtcc gaggttgcaa ccacaactac agaccttttt
    11461 agtccctcct ccttaaggtc cttctcaata aattccctga cttcccttcc tctttctcct
    11521 ataagggcta ttacgtttat atctgctttt gtattcctgg ctatcatacc tatcagtgtg
    11581 ctcttaccca caccgcttcc tgcaaaaatt cctactctct gaccctttcc tacggtcagg
    11641 agaccgtcaa tacatttaac gcccaacggc aatgggtcct tgattcggtt tctttccaac
    11701 ggctccggtg gagcattgtc aacgggatat gtacactcat aagttatttc tccctttccg
    11761 tctatcgggt ttcccatgcc gtcaatcacc ctgcccataa gatcgctgcc aacaccaatt
    11821 gacagatact cacccgatgc aacaaccttg cttcccggcc ctattccgct catattgccc
    11881 aaaggcatca aaagcacctt gttgtccttg aacccaacta cctctgcggc tattttttta
    11941 ttctccctta aagcattgat cctgcatatt tctccaatat tgacctcagg cccgtttgat
    12001 tcaattgtca gacccaccac cttggttacc tttcctatat actcaattag gtttgttttt
    12061 tccagtatat cctgatattt tgttaagttt acaacagcca taaagcctcc atatcctgat
    12121 acttaggttt ataactactt attcaaaacc ttttcaaagg actcttcaat cttctttatt
    12181 tttgtattca ctcctgagtc cacgctccca tatgcggtct ccaatatcaa gtttccaggc
    12241 tttaaggact tatcctcaat aatttcaagc ccgtctaatc cttccgtcat cgaaagaatc
    12301 atgtccttgt tttcctctat aaattcatag tcctttgaag aaacctttaa cgctatattt
    12361 tcccgatttg aacatttctc gaaggcctgc ctcaccagat tcagcaaata cccttggtta
    12421 agacttattt catcatctat taccttcctg ctgacaccta atataacatc tacggcatcc
    12481 ttttcaattc cctcgagcac ttccttatat tcctttaaag cattttcctt aaccttctct
    12541 gcctccctaa gcagatcatc atattgtttt ttggcttcct cataaccgtc ttgatagcct
    12601 ttttgtctag cttcttcctc tatcaaggac ttcaactctt ccgctttttt tcttgcattc
    12661 tcaattattc tatccgcttc gagttctgct tcctttatta tggattcagc ctcaagtctg
    12721 gtcttttcaa gaacctcctg tgcatcttcg atcttgtttt tgacagtttc aaaatcaggt
    12781 ccgatatgta ctgcattcgt ccttttgata ttctgatagt tcagaggtac tcttatttgc
    12841 accggtattc ccatattaat ctgattgctt ttaaatatcc tgttatacaa ttatttcatc
    12901 tcctccgcct ctggagatta ctatctctcc tgtttcttca agctttctga tgatattcac
    12961 gatcttctgt tgtgcctcat caacatcctt gaccctcaca ggacccatat attccatatc
    13021 ctccttcatc atctcaacaa gacgcttgga catattggag aatatcttgc tttgaacctc
    13081 ttccgaagta cctttgagag caattgcaag ttgattgttg tcaacttcac gcagaaatct
    13141 ttgaataaaa cggttgtcca tggtaagaat atcttcgaat acaaacattc ttttcttaat
    13201 ctcttccgca agatcggtgt cttcaatttc aagggtttcc ataatatatt tttcagttcc
    13261 cctgtcaaca ctgtttagaa tatccactat tgcctgaaca ccacccgcag tggtaaagtc
    13321 tcctgtaaca agggaagaaa gctttttctc aaggaccctc tcaacctcct tgattacttc
    13381 aggggttgtc ctgtccattg ttgctattct tcttgcaaca tcagcctgtt tttcctgtgg
    13441 cagtgcggac aatacaactg aagattgttg aggtttcaaa tatgccagaa tcaaggctat
    13501 tgtctgagga tgctcattct ggataaaatt caatagttgt gaaggatcgg tctttctcac
    13561 aaaatcaaaa ggttttacct gaagagaaac agtgagctta ttcaagacat ccaaagcttt
    13621 ttgcgttcct aaagccttct ccagtatttc cttggcataa tttataccac cctccgcaat
    13681 ataatcctgt gccaagcata tctggtagaa ttcctccaaa accttttcct tttcctccgg
    13741 agtcactgct ctaatattgg caatttcaag ggtaagctgc tctatttctt cctccttaag
    13801 atgtttaaaa atcgacgcgg atttctcagg tccaagagca atcaacagca ttgccgcttt
    13861 ctctttcccg gataattcca ttttggcaga tgcaactcga gccacattaa taccccctaa
    13921 gtttttattc ccagtcctct gataaccagt ttcttagaag ctgtgcaaca gactcgggct
    13981 tttctcttac aaatttttca atctgcttct tgatttccga acgttcctca aaagtaattt
    14041 caggaacagt atctcttatt tcattgtcaa tagctattgc tccatttgct gcagccaact
    14101 gaggttccag ttctgattgc tgattccctt ttctgggtat aacagcaatc ataagtgcaa
    14161 ctattaagag aatcatcaat ataaagaaac catagttatc gagaaactct ttaattcttt
    14221 cccatgctga aacaacatct tcttctgccg gagccagttt ttgcttgtcg acagatatcc
    14281 ttgaaacagg tattcccgtt gcagtgctaa tactctgttt aaactggtcg ataaattcct
    14341 gtgtcagtct gctgtcatca ggaactctgt ccccatacca taatgccact gtcatagatg
    14401 attcatcaaa atttactgtt ccaatggctt tctcctgtgc agtcatagtc tcggtaaata
    14461 ttctgttaat tatttctgaa tttttattat aagaagaatt atttccccca tctatgggat
    14521 aacccggaac ctgccctgtt cccgggtttg aatccatccc tggtgcctct ccgccataac
    14581 cattctccag ttcttcttta tgcttttcct gacttgctac agcctcatcc agtccggtag
    14641 gcttctcaat ctctttcctg tttgttgtaa ccctgtccag gtcaaggacc ggatttacag
    14701 ccacacttat aaaatcataa ctgtccgaac gacccgagta aagctttttt atgtttttct
    14761 caagctcttc cttaattcga agcttaagct tgtattgact tgatggaata ctcattccgt
    14821 cgcttaagtc ctgattcaat atattaaagt tgttgtctac taccgtaaca tttttcgggt
    14881 ccaaaccctc tatggacgat gcaactactc tgacaatccc ttctacctgc tctggagtta
    14941 tttccccttt gggctttatt cttacggatg ccttggcctc ttcctcattt tcaataaaga
    15001 acatggtatc atccggtatt gtgagctcca catcggcatc cttaatatta tcaaacatct
    15061 tcagcttggc aataagactg tttttcttga aattatgcca gagttgcttt ttgtcgctct
    15121 ccgttgtgct tacatttatt aaactccaag cgtcttcgaa ggtcattccc ttctcagtta
    15181 aacctgccga ggccaatgca aattctgctt tattcttttc ttttgaatct acaaggattt
    15241 gtcccttatc acccggttta taattaattt tcttttcctg caaggtcttt tctatttcag
    15301 aaagattttc tgagtcttca atggtcataa gcggaacata tgtagttctg gtaagcatta
    15361 ttatacctat tgtaagtgta
//
LOCUS       DAOOJC010000004        15232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_3754_length_15232_cov_3.939777, whole genome shotgun sequence.
ACCESSION   DAOOJC010000004 DAOOJC010000000
VERSION     DAOOJC010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 15232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15232
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3754_length_15232_cov_3.939777"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1827
                     /locus_tag="PLA01_00230"
     CDS             82..1827
                     /locus_tag="PLA01_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPC99758.1"
                     /translation="MRKGKKLCLLVAVLMVISVICFSTNTEPTAASVDSNNDDWLHCR
                     GNKIYDMYGNEVWLTGANWFGFNCSENCFHGAWYDVKGILTNIADRGIGFLRIPISTE
                     LLNSWMIGKPNKVSSVTASNNPPYHCVNPDFYDPETDDVKNSMEIFDIIMGFCKELGI
                     KVMIDVHSPDANNSGHMYELWYGKEGSTCGVVTTKMWIDTLVWLADKYKDDDTILAFD
                     LQNEPHGKRGYSAEMPPLLAKWDNSTDENNWKYAAEKCAKAILAANPNLLIMIEGIEQ
                     YPKTELGYTFETPDVWGATGDASPWHGAWWGGNLRGVKDYPIDLGSLNSQIVYSPHDY
                     GPSVYEQPWFQKNFTTQTLLDDYWYDTWAYINDQGIAPLLIGEWGGHMDGGRNQKWMT
                     LLRDYMIENRIHHTFWCINPNSGDTGGLVGNDWKTWDESKYGLLKPALWQSGGRFIGL
                     DHQIPLGSNGMSLGEYYGSSTPTAKPTPTTKPTPTTKPTPTTKPTPTPTQSTESPNPS
                     PTTAISTSFGDINSDGSIDSSDLTLLKRILLKKNPSGVDLGAADVNDDGTLDSTDLTI
                     LKRYLLKKITVLPYK"
     gene            complement(1958..4099)
                     /locus_tag="PLA01_00235"
     CDS             complement(1958..4099)
                     /locus_tag="PLA01_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HPC99759.1"
                     /translation="MEYLSGVVERITFINEENGFCVIKIKSRGFSELVTVVGNMASVN
                     VGTVVNLKGEWKNDAKYGKQFCVHSYKETMPATIAGMEKYLGSGLIKGIGPVNAKRIV
                     KSFGEDTIRVIEEEPDRLSEVDGIGPKRVEMIKKAWQEQKEIKNVMLFLQSNGVSTAY
                     AVKIYKTYGSDSIETVKDNPYRLADDIWGIGFKTADRIAQSMGYDRESYQRLRAGIIY
                     VLNEFANEGHCFARRDELIKRSSEMLEMGEGDVESAIRSMLEEKSIIMEYDDALYLPP
                     FYYSETGVARRIMEIVMTKSKYCSCNLGEVIQRVRKECGIEYDDVQVEAIEKAITSKF
                     MVLTGGPGTGKTTTTMAIIKAFESLKANVFLAAPTGRAAKRLSEATGMSAQTVHRLLK
                     YSPLEGYKNNEENPLYCDVLIIDETSMIDIVLMYNLLKAVSNDTVVILVGDVDQLPSV
                     GAGNVLKEVIDSGAVTVVRLNRIFRQAQGSAIIMNAHRINRGEFPALKGGKNSDFFFI
                     EEEDPEKIVGLIRQLCKERLPKYYGVDPVNDIQVLCPMLKGAVGAYNLNAVLQDALNP
                     SDISVRYGGITYKVNDKVMQIKNNYDKNVFNGDIGRITLIDNEEKVICIKFDDNVVEY
                     DVTEMDEVTLAYAVTVHKSQGSEYSIVVAPFTMQHYMMLQRNLLYTCVTRAKKVIVLI
                     GTKNALGYCIRNSKSIDRNTMLALRLQEYSR"
     gene            complement(4137..4676)
                     /locus_tag="PLA01_00240"
     CDS             complement(4137..4676)
                     /locus_tag="PLA01_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99760.1"
                     /translation="MFEFNDKEMIEIMIDSLGKYLKAAGRISKQRFDELSEHNGLLWL
                     EITKQKEILENILSRLQNEVGTDEANSSGEDNNKVSNNKVNGSIKLYDQMVDYLKKQK
                     FTELQWVRLYSVRFPQQDFDSFEIKYFEHKDILQEDKNYLLFGNATNNYVIRKSGNEI
                     IGDTNQCFEYILLFLNQFK"
     gene            5073..5495
                     /locus_tag="PLA01_00245"
     CDS             5073..5495
                     /locus_tag="PLA01_00245"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC99761.1"
                     /translation="MQILSYKLNAYIPFDKDSIEKFLFVCEDVINNMTSDEKTVFKLK
                     SATHELLINSLEHGYKKSAGEVCFSMSRKGNMIQLEMSDEGTGFDASILDIEEQNMNP
                     ETLRDRGWGLLIIKKLCDSIQISPNTPRGTKVTVSINN"
     gene            complement(5492..7249)
                     /locus_tag="PLA01_00250"
     CDS             complement(5492..7249)
                     /locus_tag="PLA01_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HPC99762.1"
                     /translation="MKTIIVAVNSKYIHSSLAPWYLKASCKNNCGTIKVMEFTINDDP
                     DSVLSRIYAEDCDVAAFSCYIWNITFVLRVAENLKKVLPAIKIVLGGPEVSYDSEQVL
                     KLHPFVDFVISGEGESAFNALLSFLNGRNQELSGIKGLSFREDGRITVSNYFPLENDL
                     DLIPSPYDEEMLFCVGNRIVYFESSRGCPFSCSYCISSTFEGVRYFSMDRVKSDLRFL
                     LTAGVKLVKFVDRTFNCNRERVKAIFEFIIDNAGNTVFHFEAAADLFDEETIEILSRA
                     PKGLIQLEIGIQSTNEASLEAVKRKTNINKVLYYVKRLMELENIHVHVDLIAGLPLED
                     YSSFKDSFNDAYNLYPHQLQLGFLKMLKGSAIREEYRKYGYKYREYPPYEVLGNEFLS
                     FEEIVKLKRIEEVLERYYNSGRFNKSLKYIINGFFYSPFEFYEELSFFYLNEGCFERS
                     ISSRELYTLLIKFIGESNKKIDLTVLNELLKFDFLVSDNTNNLPLGIERTYKDSFKTK
                     CFEFLKSGDNIAMFLPQFCGEPPKKVYNKVHFELFTYDITENDGTLPKKDTVILFNYS
                     ERDRVTGQYSFIKIQLPSD"
     gene            complement(7442..8647)
                     /locus_tag="PLA01_00255"
     CDS             complement(7442..8647)
                     /locus_tag="PLA01_00255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HPC99763.1"
                     /translation="MRKRIIIRILAFLLCLNFVLDISQEMYADDLIEEEPLTRLYTTE
                     VSSCVGTDLPQIEAGAAIVIDADSGRVLYEKNAYSRRAIASTTKIMTAIVALENGNLD
                     DTVKVSKRASSIWGSTIKLREGEELTLRELMYGMMLKSGNDAALAVAEHIGGTVENFV
                     KMMNDKAKELGLTNTSFKTPHGLDADGHYSTAYELAVLTQYALKNPVFSKIVSTQNIS
                     IGNRNLYSTNEMLSLYPGADGVKTGYTGKAGRCLVTSAKRDGFRLISVVLFCSSRAKR
                     AESSKKILDYAFSNYKPYELVKPNQKLGEVDVKRGLKNNVSVVSIEGIKIPLSEKEKE
                     ALKTEITLNNPVSSPVHMGVEVGTISFYVEGKLYAQAGIKTAESVPAKTYRHYFGDVM
                     NVWFKLVRQ"
     gene            9094..10476
                     /locus_tag="PLA01_00260"
     CDS             9094..10476
                     /locus_tag="PLA01_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPC99764.1"
                     /translation="MKEKIIISVSHDGLKATVIFNCSKDELDFKNREILVKESYSKLK
                     EKQIMYGIKKELFFSDLESGKEYIIAEGTPSVNGKDCVIRMYNLEEPKPQIREDGKVD
                     YYDLKLINKVKAGEWLGERINATEGIPGHSVHGTPIKPSKGKNYPLLYDRNTVYEVAM
                     DDKVVLYAKIGGAVNYSGGKIMVSNYLEIDGDVDFKTGNISFDGYVSIKGTVIDGFSV
                     EATKDIEINGDLGLGNVKSIKSYEGNIYIKGGIASKDVAEIYAGKNVFARFVDNVRLF
                     CGGIAHIGFYCINSTIYAKEVFLESSRGNIIGGKINAEVKVTTAILGSSLEKKTEVIV
                     QGFDRDKITEQIEDLNTRKKDLLNEQQHLKQLLAKQNAIEEPSDKDIAVYGKNLQRFA
                     ILREEIKSVDKEIKNLLRYLRAKGDGEVNITQRIYPNCSITIKNKQIEIKVPYTSAVF
                     FVTDGEFRKL"
     gene            10844..12052
                     /locus_tag="PLA01_00265"
     CDS             10844..12052
                     /locus_tag="PLA01_00265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPC99765.1"
                     /translation="MVNNILIISSNYTGHGHKSISESLIEKFSLYPDVDVHVIDGFSL
                     AGKFGLRIGKLYGSVTRNAKDLWKLMWEVSFRKPSLVIDFTETSIRDNFLKLLKTIKP
                     DLILSVHPNFNASVLNILEDFNIKIPFLTLIADLVSISPLWADPRADYIICPSKESKY
                     KCLEFGVSESKLKVIGFPVRQKFLQHTESTTGRRYYNNNMPLQCLIMSGGEGSGNMRR
                     IAKILLRNFNCHVKIVAGRNKILKRRLERSLYEQFGNRVEVFGFTENIQDLMLSSDIA
                     FTRGSPNVMMEAVACNVPLIITGNLPGQEEGNPGYMRKHNLGVVCKEPAQLKNIISDL
                     LDNNAEKLNKIREAQKKFLNPGIAKEIVDFILSMEKRGDINLPDEVPRLWDLSKKSVA
                     GRKIIVNRHK"
     gene            12191..12403
                     /locus_tag="PLA01_00270"
     CDS             12191..12403
                     /locus_tag="PLA01_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HPC99766.1"
                     /translation="MANNNSNSKTQFENMKYEIAKEVGVNLKQGYNGDLTAREAGKIG
                     GNIVKKVFQSYTGNSYPTERLGDTSR"
     gene            12673..13359
                     /locus_tag="PLA01_00275"
     CDS             12673..13359
                     /locus_tag="PLA01_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HPC99767.1"
                     /translation="MLVVVTRTLILYLLVVVIMRIMGKRQIGQLQPFELVIAIMISEL
                     ASVPMQNTGIPLINGIMPIITLLIAQLALSFIGIKSTKARAIICGRPSILVENGKIKE
                     DQLRKEMYTLNDLLEQLRIKNIPNIADVEFAILETNGQLSIIPKSQKRPVSPQDLNIP
                     TQYEGLTLDLVIDGNINYDNLKLSGLDTNWLIQKLSVFGINNIKDVLFASLDSEGNLY
                     YQVKDKGRVK"
     gene            13406..13750
                     /locus_tag="PLA01_00280"
     CDS             13406..13750
                     /locus_tag="PLA01_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HPC99768.1"
                     /translation="MILGTASYTLKLLSKDSQELESLIEMIEASTQNGDWASVENHLS
                     SLETEWHRAENAWAVLLDHFEIDNIDTSIMRVSKFVETRNVPMALSEIAVLKQYIKHI
                     PEKESFSLKNLF"
     gene            complement(13916..14911)
                     /locus_tag="PLA01_00285"
     CDS             complement(13916..14911)
                     /locus_tag="PLA01_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HPC99769.1"
                     /translation="MGINAAQMFMALVLILISCEFFTNGVEWLGKKLKVGDGVVGSVF
                     SAVGTCIPETIVPIVAIVFLKEDKKTVDVGVGAILGAPFMLSTLAFFVMGVSVMVFLS
                     KRRTKMTMEINKDVLSWDISFFIVTYTIGVLTSFIKIRLLEEIVSAFLVLAYVYYVIV
                     TVKRDKGGHGYPDKLYLSRILNLRPNIQNIVLQVSIALAGVIFGANVFVRNIEIISNF
                     IGISPVILSMIITPIATELPEKFNSIIWISKNKDTLALGNITGAMVFQSCIPVSIGIL
                     ATPWNLDNHIIVSSVIGTLSAAVTYFWIEDKGKLNPIPLLMGGIFYAMFILSLFI"
BASE COUNT         4969 a         3016 c         2704 g         4543 t
ORIGIN      
        1 atactgctaa aaccctttgg tttcaagggt tacagcattt tacaaacaac taaataaatt
       61 ttatatgggg gaaagaatta tatgagaaaa ggaaaaaaat tatgtttact tgttgctgta
      121 ctaatggtta tttccgtgat ttgtttttcg acaaacaccg aaccgacggc cgcttctgtt
      181 gacagtaaca atgacgactg gttgcactgc aggggcaaca agatttacga catgtacgga
      241 aacgaagtgt ggcttacagg agccaactgg tttggcttca actgcagtga aaactgcttc
      301 cacggtgcct ggtatgatgt taaaggcatc ctgaccaaca tagccgacag gggtattgga
      361 tttttaagaa tacccatctc caccgaactg cttaacagct ggatgattgg caaaccgaat
      421 aaggtatcca gtgtaacagc cagcaataat ccgccatatc attgtgtaaa ccctgatttt
      481 tatgaccctg agaccgacga tgtaaaaaac agtatggaaa tatttgatat tataatgggt
      541 ttctgcaaag aattaggtat taaggtaatg attgatgtac atagcccgga tgccaataat
      601 tcaggacaca tgtatgaact gtggtatggc aaagaaggaa gtacatgcgg agttgttact
      661 acaaaaatgt ggatagatac tttagtctgg ttggctgaca aatacaaaga tgatgacacc
      721 atattagcct ttgatcttca aaatgagccg cacggtaagc gtggatattc ggctgaaatg
      781 cctcctttgc ttgcaaaatg ggacaattcc accgacgaaa acaactggaa atacgctgca
      841 gaaaagtgtg caaaggcaat ccttgctgca aacccaaatc tgttgataat gatagagggt
      901 atagaacaat atcccaaaac tgaattaggt tatacctttg agacccccga tgtatgggga
      961 gcaaccggtg atgcatctcc gtggcatggt gcatggtggg gaggtaattt aagaggcgtt
     1021 aaagattatc ctattgattt aggatcttta aacagtcaga ttgtgtattc tcctcatgat
     1081 tacggtccgt cagtatatga acagccgtgg ttccaaaaga attttacaac tcaaacttta
     1141 ttggatgatt actggtatga tacctgggca tatattaatg atcagggaat cgcaccactc
     1201 cttataggtg agtggggagg acatatggac ggcggtagaa accagaaatg gatgactctt
     1261 ctgagagatt acatgattga aaaccgtata caccacacat tctggtgcat aaatccgaat
     1321 tcaggagata ccggaggatt agtaggtaat gactggaaaa cttgggatga atcaaaatac
     1381 ggattattaa aaccagcgtt atggcaatcc ggtggcaggt tcatagggct tgatcaccaa
     1441 ataccccttg ggagtaacgg aatgtcattg ggtgagtatt atggctcatc tacacctact
     1501 gcaaagccca cacctactac aaaacctaca cctactacaa aacctacacc tactacaaaa
     1561 cctacaccta cacctacaca atcgacagaa tcaccgaatc caagcccaac aactgctata
     1621 tctactagtt ttggtgatat aaatagtgac ggttccattg actcttccga tttaacgctg
     1681 ttaaaaagaa ttctgttaaa gaaaaatccg tccggggtag acttaggggc agctgatgta
     1741 aatgatgacg gtactttgga ttcaacagat ctaacaattc taaaaagata tttattgaaa
     1801 aagattactg tacttcctta taaataaagc aagctgtaat tagaatatta ttaaatcgat
     1861 taaaagcgat gggggcatca ggcctccatc gctttttggt atgcattaaa ccctccaagg
     1921 catttatttt gtacttactg ttgacgggtg taaaaggtta ccttgaatac tcctgcagtc
     1981 tcagagcaag catggtgttt ctatcaatgc ttttagaatt tcttatacaa tatcccagtg
     2041 cattttttgt accgataaga acaattacct ttttcgccct ggtcacacat gtatacaaca
     2101 ggtttctctg aagcatcatg taatgctgca tcgtaaaagg tgctactaca atgctgtact
     2161 cgcttccctg acttttatgt actgtaacgg cataagccag agttacctca tccatttccg
     2221 taacatcata ctccaccaca ttgtcgtcaa actttatgca tataaccttt tcctcattgt
     2281 ctataagagt tatccggcct atgtcaccgt tgaatacgtt tttgtcataa ttgttcttta
     2341 tctgcataac tttatcattg actttgtaag ttataccacc atacctgaca gaaatatcgg
     2401 aaggattcag agcatcctga agaacagcat ttaaattata tgcacccaca gctcctttga
     2461 gcattggaca tagaacctga atatcgttca ctggatccac tccatagtat ttgggtagac
     2521 gctctttgca taactgacgt ataagtccca caattttctc cggatcttcc tcttcaataa
     2581 agaaaaaatc actgttcttt ccgcccttta gtgcgggaaa ctctccacga tttattctat
     2641 gtgcattcat gatgattgca ctcccctgcg cttgacggaa aattctgtta agtctcacaa
     2701 cagtaactgc accggagtca atgacttctt ttaaaacatt tcctgccccc actgaaggaa
     2761 gctggtctac gtctcccacc agaatcacca cggtatcgtt ggaaactgct ttcaaaagat
     2821 tatacataag aacaatgtca atcatagaag tctcatcaat tatcagaaca tcacagtaca
     2881 agggattttc ctcattattt ttatatccct ccagcgggct gtatttcaat aaccgatgca
     2941 cagtctgtgc actcatgccg gtagcctcag acaacctctt tgctgccctt ccggtaggtg
     3001 cagcaagaaa tacattagcc ttaaggcttt caaaagcttt tataatagcc atggttgttg
     3061 tagtctttcc cgtgcccggt cctcctgtta gaaccatgaa ctttgaagta atggcttttt
     3121 caatagcctc aacctgcaca tcatcatatt cgattccgca ttccttccga actctttgaa
     3181 ttacttcacc caggttacaa gaacagtatt tactttttgt cataacaatc tccattatcc
     3241 ttcttgctac ccctgtctcg ctgtaataaa agggcggaag atacaaggca tcatcatact
     3301 ccataattat ggatttttcc tcaagcatgc tccgaatagc cgattcaaca tccccttccc
     3361 ccatctcaag catttccgag gacctcttta taagctcatc ccttcttgca aaacaatgac
     3421 cttcattggc aaactcatta agcacatata ttattcctgc ccttaaccgc tgataggatt
     3481 ctctgtcata ccccatactt tgagctatcc tgtcggcagt cttaaatcct attccccaga
     3541 tgtcatccgc caacctatag ggattgtcct ttacagtttc tatgctgtcg ctcccgtaag
     3601 ttttatatat ttttacggca tatgcggtag acacaccgtt ggactgcaaa aaaagcatga
     3661 cattttttat ttccttctgt tcctgccatg ctttttttat catttcaacc cttttcggtc
     3721 caattccgtc aacttcactc aacctatccg gttcctcttc aataaccctt atagtgtctt
     3781 ccccgaaaga cttaactatc ctttttgcgt ttacaggccc aatcccttta ataagaccgc
     3841 ttcccaggta tttctccata ccggcaatag tagcaggcat ggtctcttta tagctgtgca
     3901 cacaaaactg ttttccatat ttggcatcgt ttttccactc gcctttgagg tttaccacgg
     3961 ttccgacatt gacggatgcc atgttaccca cgactgtcac aagctcggaa aatcccctgg
     4021 attttatttt tattacgcaa aaaccattct cttcatttat aaaggttatt ctctccacta
     4081 ctccactcaa atactccata agccattacc cgaccaatag tttattgtat ttaccgctat
     4141 ttaaactggt tcaaaaaaag cagtatatac tcaaagcatt ggtttgtatc cccgatgatt
     4201 tcatttccgc ttttgcgtat gacgtaattg tttgttgcat ttccgaacaa aaggtagttt
     4261 ttgtcttcct gcaaaatgtc cttatgctca aaatatttta tttcaaaact atcaaagtcc
     4321 tgctgtggga acctcacaga atataacctc acccattgca gttcagtgaa cttttgtttt
     4381 ttgagataat ccaccatctg gtcatacaac tttatcgaac cgttaacctt attgttggaa
     4441 accttattgt tatcttcccc ggatgaattt gcttcatccg tgccaacttc attttgaagc
     4501 cttgacagaa tattctcaag aatttccttc tgttttgtta tctcaagcca taaaagcccg
     4561 ttatgttccg acaattcgtc aaacctctgc tttgatattc gccctgctgc cttaagatat
     4621 ttgcccaaag aatcaatcat aatctcaatc atttctttat cattaaactc aaacactacg
     4681 ttcacccttt atcgataaat ttaaattaaa tttatatata taatatatca aattttttcc
     4741 gtcaaaacaa acataacctt aaggtacata ttatttaagt gcgaaaatgt gaaagaaacc
     4801 taagtttttg gaacattttt cttaaaagtt atttaatgta tccataactt tttattgcat
     4861 ttaattttca aatgctttat gatataatca aacatatgtt aagaacgagg caaaaaattt
     4921 acccgcctgt attttggtcc gcgggtgccg gtttgttttc agcatatctc ccgcctcgtt
     4981 gaaacgctta tgggcagtaa attttccgca gccggagaat ttcattttaa aacctataag
     5041 ttacaagtat tctgcttatg gaggatctgt atatgcaaat attaagttac aagctcaatg
     5101 cctatattcc tttcgataaa gacagtattg agaagtttct gttcgtgtgc gaagatgtga
     5161 taaacaacat gacatcggat gaaaagactg tttttaagct caaaagtgcc actcatgaac
     5221 tactcattaa ttctcttgag catggataca aaaaaagtgc cggtgaagtc tgctttagca
     5281 tgagcaggaa gggaaatatg atacaacttg aaatgtcgga tgaaggaact ggtttcgatg
     5341 catcaatatt ggatattgaa gagcagaaca tgaatccgga aacattaaga gacaggggtt
     5401 ggggactatt aataataaaa aagctctgtg acagcattca aatatcacct aacaccccca
     5461 gaggtacaaa agtaaccgta tcaataaata actagtccga tggaagctga atttttataa
     5521 agctgtattg tcctgtcacc cggtcccttt cactgtaatt gaaaaggatt acagtatcct
     5581 tctttggcaa cgttccgtca ttttcggtga tatcatacgt gaagagctca aaatgaacct
     5641 tgttgtatac ttttttaggc ggttcgccac agaattgcgg aagaaacata gctatattat
     5701 ccccagattt cagaaactca aagcattttg tcttaaagct gtccttgtaa gtgcgttcta
     5761 ttcctaaagg aagattattg gtattgtcag aaactaaaaa atcaaatttt aacagctcgt
     5821 tgagaactgt caaatcgatt ttcttattgg attccccaat aaactttatg agtaacgtat
     5881 atagctctct gcttgaaatt gaccgttcaa agcagccttc attcaaataa aaaaatgaaa
     5941 gctcctcata aaattcaaag ggtgaataaa aaaatccgtt gattatgtat ttaagtgatt
     6001 tattaaacct gcccgagtta taataccgtt ccaaaacctc ctctattctt ttgagtttaa
     6061 caatttcctc aaagcttagg aactcattgc ccaaaacctc ataaggagga tattcccgat
     6121 atttatatcc atatttgcgg tattcctccc ttatggcaga acccttcagc atctttaaaa
     6181 aacccaactg cagttggtgg ggatataggt tataggcatc attaaaagag tccttgaatg
     6241 aagaatagtc ctccaaagga aggccggcga taagatccac atgaacatgt atgttttcca
     6301 gttccataag ccgttttaca tagtataaca ctttattaat attcgttttc ctttttacag
     6361 cctcaaggga ggcttcattg gttgattgta taccgatttc gagctgaatc aatccctttg
     6421 gtgccctgga caatatttca attgtctctt catcaaacag gtcagctgcc gcttcaaagt
     6481 ggaaaaccgt attcccggca ttatcaataa taaactcaaa tatagccttc actctttccc
     6541 tgttacagtt aaacgttctg tctacaaact ttaccagttt aacccccgct gtcaacaaaa
     6601 aacgaagatc cgacttaacc ctgtccatag aaaaataccg tactccttca aaggttgaag
     6661 aaatacaata tgaacaggaa aacgggcatc cccttgagga ttcaaaataa acaattctgt
     6721 ttccgacaca aaagagcatt tcttcgtcat atggagaagg aatcaaatca agatcattct
     6781 cgagaggaaa ataattcgaa acggttattc tcccatcttc tctaaagctt aagcctttaa
     6841 tgccagaaag ctcctgattc cttccgttca aaaaactaag taaagcatta aaagcacttt
     6901 cgccttcacc ggatatgaca aagtccacaa acggatgcaa cttcaaaact tgttcggaat
     6961 cgtatgaaac ctcggggccg ccaagtacta tttttatagc cggtaatact ttcttcaggt
     7021 tttcagccac tctaagaaca aaggttatat tccagatata gcaagaaaaa gcagcaacat
     7081 cacagtcttc agcatatata cgcgacagaa cggaatccgg gtcgtcattt atggtaaatt
     7141 ccattacctt tatcgtcccg cagttattct tacaggatgc tttaaggtac catggtgcca
     7201 gtgatgaatg tatatattta gagtttacag caacaattat tgtcttcata tgtaaataaa
     7261 atctccttaa aattatacgc taagttatta tagcaaacct ggctataaac attcaaggat
     7321 aagttcatca ggtgcataat tgataaatgc gaataaaaaa acttaaattt ccggaatatt
     7381 ttccttcgca ttttacccaa caacagtgag tatataaaac aaagatgaag aaaatatacc
     7441 ttcactgtcg aaccagttta aaccacacat tcataacatc tccaaaataa tggcggtatg
     7501 tttttgccgg cacactttcc gcagttttaa tccctgcctg ggcatacagc ttaccctcaa
     7561 catagaaact aatcgtacca acttccacac ccatatggac aggtgagcta acagggttat
     7621 tgagtgttat ctcggttttc aatgcctcct tttctttttc gctcaaaggt attttaattc
     7681 cttcaatgga cactactgaa acattatttt tcaaacctct ttttacatca acctccccaa
     7741 gcttctgatt gggttttaca agctcatagg gcttataatt gctaaatgcg taatccaaaa
     7801 tttttttgct gctttctgcc cttttagccc ggctggaaca aaacaaaaca acagaaatta
     7861 atctaaaacc gtcccttttt gccgatgtca caagacacct tccggccttt ccagtataac
     7921 cggtctttac tccatccgca ccgggatata gtgaaagcat ctcatttgtt gaatacaagt
     7981 tcctgttgcc aattgatatg ttctgtgtac tcacaatctt tgaaaaaaca ggatttttaa
     8041 gtgcatattg agtaagaacc gccagttcat aggcagtaga atagtgaccg tctgcatcaa
     8101 gcccatgggg agtcttaaag gacgtattcg taagccccag ttcctttgcc ttgtcattca
     8161 tcattttaac aaagttttca acagtccctc caatatgttc ggcaacagcc aaagctgcat
     8221 catttcctga ctttagcatc atgccataca taagctccct taatgtcagt tcttcccctt
     8281 cccgaagctt aattgtcgaa ccccaaatgc tcgaggccct cttgctaacc tttaccgtgt
     8341 catcaagatt gccgttctct aatgccacaa tagcagtcat aatcttggtg gtactggcta
     8401 tagcccttct ggaatatgca ttcttctcat acaaaaccct tccgctatcg gcatcaatta
     8461 ctattgccgc accggcttct atttgcggca gatcggtacc tacacaactg ctgacttcag
     8521 tagtatataa ccgggtaaga ggttcttctt caatcaagtc atcagcatac atttcttgac
     8581 ttatgtccag tacaaaattt aaacagagta aaaaagctaa aattctaata attattcttt
     8641 ttctcaaata tatcacatcc taagtaaata ttattctatg ctttaagtgt ttagtttaaa
     8701 gtttatttgg gtaagtatat aatattcaat tatattttta tagtacatat actgtaaaat
     8761 cacccaatat gtgttctgta ttcacattct atacatagat gtaatggtat aaatatatga
     8821 attaccgcct atgtttataa atatttttta ttaaaaaacc gctaataaat acgaggggga
     8881 tgatatatga ttgatcaaac catttacgaa gatcaatata ttaaaatatt cacacgatca
     8941 agagacgtaa tgattgaatc atttaagcct ggattatcat tggacaaagt tcacaaaata
     9001 atatcctcgc atccacaaat tcaaataacc agttttaatt gtattaagca cgctctaatc
     9061 aacgcaccaa catcccctag taaatttggc gagttgaagg aaaaaattat tatatccgtt
     9121 tcccatgatg gattaaaggc tactgtcatc tttaattgct caaaagatga attggatttt
     9181 aaaaatcgcg aaatacttgt aaaagaatcc tatagcaagt taaaagaaaa gcagataatg
     9241 tatggaatca aaaaagaact ctttttcagt gatttagaaa gcggtaagga atacattatt
     9301 gcagaaggaa caccaagtgt aaacgggaaa gactgtgtaa tcagaatgta caatcttgaa
     9361 gaacccaaac ctcagataag ggaagacgga aaggtagatt attacgattt gaagttaata
     9421 aacaaggtta aggccgggga atggctcgga gaacgaatta acgctactga aggtattccc
     9481 gggcattcag tacatggcac tccaataaaa ccgtcaaaag gcaaaaacta tccgttgctg
     9541 tatgatagaa atactgttta tgaagtggca atggacgata aagttgtttt atatgcaaaa
     9601 atagggggag cagtgaacta ttccggcggc aaaataatgg tatcaaacta tcttgaaatt
     9661 gacggtgacg tagattttaa aaccggaaat attagttttg acggatatgt atccataaaa
     9721 ggaacggtta tcgacggttt ctcggtggaa gccaccaagg atattgaaat aaatggagat
     9781 ttaggtcttg gaaacgtaaa atcaataaag agctacgagg ggaatattta cattaagggt
     9841 ggcattgcat caaaggatgt agctgaaatc tatgccggta aaaatgtctt tgcaagattt
     9901 gtggataatg tgcggttgtt ctgcggaggt attgcccata tcggttttta ctgtataaac
     9961 agcacaatct atgcaaaaga agtgtttttg gaatcttcaa ggggaaacat aatcggagga
    10021 aaaataaatg cagaagtaaa ggttaccaca gcaattttag gatcctcact cgaaaagaag
    10081 actgaagtaa tagttcaagg ctttgacaga gataaaataa cagagcaaat tgaagatctg
    10141 aatacgagga aaaaagattt attgaacgaa caacagcatc tgaagcaatt gctggcaaag
    10201 cagaatgcca tagaagaacc aagcgataaa gacattgcag tatacggaaa aaaccttcag
    10261 cgttttgcga tattaagaga agaaatcaaa tctgttgata aagaaataaa gaatctgtta
    10321 aggtatttaa gagcgaaagg tgacggtgaa gtaaatataa cgcaaagaat atatcccaac
    10381 tgcagcataa ctataaaaaa caaacaaata gaaattaaag taccatacac atccgctgtt
    10441 ttttttgtaa cggatggaga gttcagaaaa ctctgattga ctttgataca aagtattttt
    10501 aattgctaat ccaataataa aaaatacagc ataagggcag catgccctta ttatttttta
    10561 cataaatctc tcataatccg gagtctcccc tgcttttttc cctttgactt tttgaaatca
    10621 tattattaat cgccggtaac tattcatatg caatatttgt cctgccatta tcgaaaattg
    10681 ttgattacag cgaaaattta cattgttata ttgttaatat ataatagcaa aaaaatatat
    10741 actaaaatat gattattcaa ccctttaatt tggaaaagta aagtaatata atatagcata
    10801 ttataatata tactttttta aaagtgatca ggaggcaata tgaatggtta ataatatttt
    10861 aataatatct tcgaattata caggacacgg tcataaaagt attagtgaat cattaatcga
    10921 aaagttttcg ctttatcctg atgtggatgt ccacgttatc gacggttttt ctttagcggg
    10981 aaaattcggg cttcgtatag gaaagcttta cggctcggtg acaaggaacg ccaaggactt
    11041 gtggaaatta atgtgggagg tatcctttag aaaacccagt ttggtaattg attttactga
    11101 aacttctatt agagataatt ttctaaaact tcttaagacc attaaaccgg atctgattct
    11161 gtcggtacat ccaaacttta atgcatctgt tttaaacatt ctggaggatt ttaacattaa
    11221 aataccgttt ttaacattga ttgcggatct ggtaagtata tctcctctat gggcagaccc
    11281 aagagcggat tatataatat gtccttcaaa agaatcaaag tacaaatgtc ttgagtttgg
    11341 agtatccgaa tcaaagctca aggttatcgg cttcccggta agacaaaagt ttttgcagca
    11401 tactgaaagc acaaccggaa gaagatacta taataacaac atgcctttac aatgccttat
    11461 tatgagcgga ggcgaaggct cagggaacat gagaagaatt gcaaaaatac tgcttaggaa
    11521 tttcaactgc catgtaaaaa tagttgccgg cagaaataaa attttaaaga gaagacttga
    11581 aagatctctt tatgaacagt ttggaaacag ggttgaggta tttggtttta ccgaaaacat
    11641 acaggattta atgctttcct cggatattgc tttcacaaga ggaagtccaa acgtaatgat
    11701 ggaagccgtg gcctgcaatg ttcctcttat tatcaccgga aatcttcccg ggcaggaaga
    11761 gggtaatccc ggttatatgc gaaaacacaa ccttggagtt gtgtgcaaag aacctgcaca
    11821 attaaagaat attatttcag accttcttga caataatgct gaaaaactaa acaaaataag
    11881 ggaagctcag aaaaaattct taaatccggg tatagcaaag gaaatagtcg attttatttt
    11941 gagtatggaa aaaaggggag atataaattt gcctgatgag gtcccaagac tatgggatct
    12001 aagcaaaaaa tcagttgccg gaagaaaaat aatagttaac aggcataaat aaaactgcaa
    12061 gatagatatg tgaacatata tatgtaactt atgaaaaaag aagttaacgt aaaacaatga
    12121 aaggatagca aaacttacta ctcataatag tcttgttttt agaaaaaata agactaagga
    12181 ggtgatagta atggcaaaca acaatagcaa cagcaaaact caatttgaaa acatgaagta
    12241 tgaaatagct aaagaagtgg gcgttaactt aaaacagggt tacaatggag atttaactgc
    12301 aagagaagct ggtaaaatcg gtggaaacat cgttaagaaa gtattccaat cttatactgg
    12361 aaacagctat cctacagaac gtttaggcga cacttcaagg taatatatga aataaaaata
    12421 gcaggaggca atctcctgct atttttattt agtgggattt cacgaagtga tattacaaaa
    12481 agcaatgggg gggctcaatg cccccggagc ttttgtatag tggaatttca cgaagtaaat
    12541 tttcactata caattcaacc ggttgtttca ggcgtagaaa tctataaaca ctgtcttgac
    12601 ataaaagcgt gattaaaaaa acatattatg tgcacactaa ataaatagaa ctaagtaaga
    12661 aaggatggtt gtgtgttagt agttgttacc agaacgctga tactttattt gcttgtagta
    12721 gtaataatga gaataatggg taaacggcaa ataggacagc ttcagccctt tgaacttgtc
    12781 atcgctataa tgatatcgga gttggcctca gtacctatgc aaaacacagg aataccatta
    12841 ataaacggta ttatgcctat tatcacactg ttaatcgctc aactggcttt gtctttcatc
    12901 ggaataaaga gcacaaaggc cagagcgata atatgcggca ggcccagtat tctggttgag
    12961 aatggaaaaa tcaaggaaga tcagctcagg aaagaaatgt ataccttaaa cgaccttctg
    13021 gaacagctga ggattaaaaa tataccgaac atagctgatg tcgagtttgc cattcttgaa
    13081 acaaacggtc agctcagtat aatacccaaa tcgcaaaaac gaccggtaag tccgcaagac
    13141 cttaatattc ccactcaata tgagggttta actctggatc tggtaattga cggcaatata
    13201 aattacgaca accttaagct ctctgggctt gacacaaact ggcttattca gaagctgtcc
    13261 gtttttggga ttaataatat taaagatgta ctttttgcca gcctggactc agaaggaaat
    13321 ctctattatc aagttaagga taaaggaagg gtgaagtaat ttgcataccg gcaaaatatt
    13381 aataattatt ggtatattat gtgttatgat attgggtact gcatcctata cattaaaact
    13441 cttgtcaaag gattcccaag aacttgagtc tctcattgaa atgatagagg ccagcacaca
    13501 aaacggcgat tgggcatcgg ttgaaaacca tctttcttct ttagaaacgg aatggcacag
    13561 agcagaaaat gcttgggcag ttttactgga ccattttgaa atcgacaata ttgatacgtc
    13621 aatcatgagg gtatcaaagt ttgttgaaac aagaaatgtg cccatggcac tgtctgaaat
    13681 tgctgttctt aaacaatata taaaacacat tcccgaaaaa gaatcttttt cgcttaaaaa
    13741 cttattctga tgcattaatc aaaaagtgaa ctagttcgga taggtctcaa taagcaaaaa
    13801 cccatggggc ttcgagcccc tttgcttttt acccgtttca ccctgtaata tccccgggtg
    13861 catgctattt tcatatttgt ttaatacacc ctgagtataa acctataacc gtattttata
    13921 taaaaagaga caatataaac atggcataaa atatgcctcc catgagaagc ggtattgggt
    13981 ttaattttcc cttgtcctcg atccaaaaat atgttacagc ggcagataga gtgccaatta
    14041 cagagctaac aataatatgg ttatccaaat tccacggtgt tgcaaggata ccgattgaaa
    14101 cgggaatgca actttggaat accattgcac cggttatatt ccccagagcc aatgtgtcct
    14161 tgtttttact aatccagatt atactgttga atttttcggg aagctctgtg gcaataggag
    14221 ttattatcat ggaaagaata acaggcgata tgccgataaa atttgatata atctctatat
    14281 tccttacaaa aacattagct ccaaaaatta cacctgccaa tgcaatactt acctggagaa
    14341 ctatattttg aatgttaggc ctaagattca atattcttga taaatagagt ttgtccggat
    14401 acccatgacc tcctttatca cgtttcactg tgacgataac ataataaaca tatgctaaaa
    14461 ccagaaatgc cgatactatt tcctccaata agcggatttt tataaaagac gtcagaactc
    14521 caatggtgta cgttacaata aaaaagctaa tatcccagct caatacatcc ttgtttattt
    14581 ccattgtcat ttttgttctt ctttttgata gaaaaaccat aacactaaca cccatcacga
    14641 aaaaagcaag ggtactcaac atgaacggtg ccccaagaat ggcacctact cccacgtcaa
    14701 cagttttctt gtcctctttt aaaaaaacaa tggcaacaat cggtacaatt gtctctggaa
    14761 tacaggtgcc tactgccgaa aatacgcttc ctacaactcc atctcccacc ttcagctttt
    14821 tcccaagcca ctcaactcca ttggtaaaga actcacagct gattagaatc agaacaagtg
    14881 ccataaacat ctgtgctgca tttatcccca tagaccatcc ttctctttcc ggtgctcttt
    14941 aacataaata cttctttaat atattagccg gtcaagagtt ttattaataa aaaacaaaga
    15001 taatgtttct tccgtatttt cctttaccat gtgtcttaca acagtgagta taaaaaagaa
    15061 gcctttaaca tataaattta tgttaaaagc ctcttacaag gacggattat ccgatgtacc
    15121 gcagctactt cctcataatc catatcggca tattccaggc tttaagtaat ggctcaaaca
    15181 gcagtattta ttggtactac tggtaacata caatataaaa acagcatgtt at
//
LOCUS       DAOOJC010000005        15064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_3808_length_15064_cov_3.751816, whole genome shotgun sequence.
ACCESSION   DAOOJC010000005 DAOOJC010000000
VERSION     DAOOJC010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 15064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15064
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3808_length_15064_cov_3.751816"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="PLA01_00290"
     CDS             complement(<1..1312)
                     /locus_tag="PLA01_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HPC99770.1"
                     /translation="MKDKTDLQRKLLSIDGAGYKAYKDIKGEYKFDRYILAIDHVQGD
                     PFASPSKVRVIVSQKDAGFPGTLYDKPHKRIGVIDFLTRLFFNNIKKYYNPSGGSGKS
                     GLISIDRCGQEILDRTSAVINEDKVEVRFEVGLPANGRRVLGKYADTIFSGAIPQIVE
                     KTLFYSNIDANALKKQVDLCEDQAFLREQIKAKGLVAFIANGSVLPRESGISDRPLSK
                     GAIPFKSPESLEVEINLPHKGIVKGMGIPEGVTLIVGGGYHGKSTLLHALELGVYNHI
                     SGDGREYVAASTDAVKIRAEDGRGVEKVNISPFINNLPNGQDTKIFSTENASGSTSQA
                     ANIIEALEIGTDLLLIDEDTSATNFMIRDERMQKLVSKDREPITPFIDKVRQLYKEKG
                     VSTIIVVGGSGDYFDVADLVIMMDEYTPKDVTKNAREIVNAHPSK"
     gene            complement(1503..1994)
                     /locus_tag="PLA01_00295"
     CDS             complement(1503..1994)
                     /locus_tag="PLA01_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99771.1"
                     /translation="MSIESMLMFILTLILIPVLLVASNDTLFFIVSGVILVFAALKSL
                     YNILFGTDMEFDESEPGVLDEIEDLISFDLNKFRVLYNNAKSILVIVFFVYCTFYLNQ
                     LWLKFLCAFVIVYWLGSIIHNLKFDENQEFKYTVPQLLVFLVDLSSIILISFSAYHKF
                     FVF"
     gene            complement(1994..2575)
                     /locus_tag="PLA01_00300"
     CDS             complement(1994..2575)
                     /locus_tag="PLA01_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPC99772.1"
                     /translation="MISVLLVIITALFSIIPTASAAEDIPQLQQDNANTLKALGILKG
                     YEDGSLRLENKIRRSEFITLVVKLMGYDTDADIDNVKVTFTDISSTHWAYNNIKLSIK
                     YNLVAGYPNNTIAPDNDVTYAEALAVVIRALGYEKTLSGEWPNNVMDKATEINLTTNL
                     SLDPNHKLTRGEMSVIVYNALTVNFNQEETGDN"
     gene            2827..3369
                     /locus_tag="PLA01_00305"
     CDS             2827..3369
                     /locus_tag="PLA01_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99773.1"
                     /translation="MAKTIAAIFDNYNNAQRAAQQIKDQGLKTDDISIVTKQGDETDA
                     GNNYSMGIGNMGGNMTANNTGENNQGGVNDNISNGVVTGGILGGLAGLLIGAGSMVIP
                     GLGIIAAAGPITGLLSGAITGGVVGGLVDLGIPEDKSRQYETDIKAGKTLFSMKVDDD
                     KIEEVSSILRNNGAASVDSY"
     gene            complement(3527..5197)
                     /locus_tag="PLA01_00310"
     CDS             complement(3527..5197)
                     /locus_tag="PLA01_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HPC99774.1"
                     /translation="MTDTVIQNKSFNSRARIILISLLILLAVLLVVFSFAVYFTLSYD
                     KAHVGIFINGIDVSKMSYEDINNHLNSNFTEKLKDTKVVLKKGDISEEFSLSQLNVQY
                     DIEGAAQKAFEVGRKGNIFQRLANIIGTKLNGLNIDLDYSFDENSVKSVIDSFYSKSL
                     TPVKETDVLIEESKVMLQTGHPGESIDTEKAYEIIENSIKSCTGGIFDIPSITITPKS
                     IDADEIYNKIVCEPSNAKATVEDNKVVVIPHVPGRKIDKSELLSLINEHAHKTDAEII
                     LPVTFEQPEINSTEVNSLLFKDKLSSASTHFSTSGTSNYNRGINISVAASKINGKILG
                     PGEVFSFNEVVGPRTVQNGYKTAKEYVGGKIVDGIGGGVCQVSSTLYNAVLFADLETV
                     SRQNHMFSVSYLPLGRDAAVSYNDLDFKFKNNSNWPIKIESSVKSGTITFTIYGTQEE
                     PGKTIELSHVQISSSPAPIQYIDDPSLAEGQTVVVQSGHSGYVVDTYKTVKINGTVIS
                     KTKLHRSTYSPYTTIIKRGTKKVEVTATPVPQPASGPSYEVVDETLFQ"
     gene            5626..5925
                     /locus_tag="PLA01_00315"
     CDS             5626..5925
                     /locus_tag="PLA01_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaL family protein"
                     /protein_id="HPC99775.1"
                     /translation="MDLNAQKTQSVKLLKNSIFSKINSLLKEKSETIYTYNTGENEDL
                     IESINDAKKEWLSARNNFEQAVESEIIDYYIYKIKAYQIRYEYLLKKAKEKGISV"
     gene            6068..6340
                     /locus_tag="PLA01_00320"
     CDS             6068..6340
                     /locus_tag="PLA01_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HPC99776.1"
                     /translation="MFQGYQTVFAYIIGIILLYFLGRFLTSPMKIAVRLILNSLPGVV
                     SLLLVNLAGSFLSYHIALNPVTVFIAGILGVPGVLLLVVLKQVFQV"
     gene            6617..7195
                     /locus_tag="PLA01_00325"
     CDS             6617..7195
                     /locus_tag="PLA01_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPC99777.1"
                     /translation="MGKVVEIRWHGRGGQGAKTASLLLADAAFNTGKYIQGFPEYGPE
                     RMGAPITAYNRISDERLSIHSNIYEPDYVVVVDDTLLASVDVTAGLKEDGAIIINTSK
                     SPEEVRPLLKGYKGKVCTIDAKTISIETLGKYFPNTPMLGAVVKVSRVMDEKEFLEDM
                     VGSFKHKFASKPEVIEGNIKALERSLQEVKGL"
     gene            7192..7497
                     /locus_tag="PLA01_00330"
     CDS             7192..7497
                     /locus_tag="PLA01_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003379678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPC99778.1"
                     /translation="MSKKELKTVKPDVNWREITPGGVIDSSGNAHLFKTGDWRSMKPV
                     WNTEKCKQCLLCHPVCPDSSILVIDGKMSDIDYDHCKGCGVCTKVCPFKAIDFVEEA"
     gene            7513..8697
                     /gene="porA"
                     /locus_tag="PLA01_00335"
     CDS             7513..8697
                     /gene="porA"
                     /locus_tag="PLA01_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HPC99779.1"
                     /translation="MAIRERLSGNEATAIAMKQINPDVVAAFPITPSTEVPQYFSAFV
                     ADGTVDTEFVAVESEHSAMSACIGAQAAGGRAMTATSANGLALMWELLYIAASSRLPI
                     VMAAINRALSGPINIHNDHSDTMGARDSGWIQLFSETNQEAYDNMLMAHRIAENPDVM
                     LPLMVCQDGFITSHAIENIELLEDDKVKAFVGEYKPQYYPLNKETPIAVGPLDLQPHY
                     FEHKKQQAEAMKNAKKVILEVSEEFYKLTGRKYGLFEEYKTDDADVVVVLLNSTAGTA
                     KYVADQFRAQGKKVGVIKPRVYRPFPVDEIAEALSKFKAVAVLDKADSFNAAGGPLFT
                     DVTSALYSKGVFGPKVINYIYGLGGRDVKADDIEVVYNKLLDIAKTGKVESVYNYLGV
                     RE"
     gene            8711..9646
                     /locus_tag="PLA01_00340"
     CDS             8711..9646
                     /locus_tag="PLA01_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPC99780.1"
                     /translation="MAYNVKEAAQKPDRLTGGHRMCAGCGAPVVVRNVLKALKPEDHA
                     VIGSATGCLEVSTFIYPYTAWKDSFIHNAFENAAATVSGAEAAYVALKRKGKLDGDTK
                     FIAFGGDGGTYDIGLQSLSGAMERGHDMVYVCYDNGAYMNTGIQRSSATPKYADTTTS
                     PAGKKIPGKLQPRKDLTEVMANHHLPYVAQTALIGNMKDLYEKSEKAIYTPGPAFLNV
                     LAPCPRGWRYNTPDLMEVSKMAVETCFWPLYEVVDGKYNITYKPKNKLPVRDFLKMQG
                     RFKHLFKAGNEHMLEEIQQEVDRRWEALLRLAGEA"
     gene            complement(9846..9920)
                     /locus_tag="PLA01_00345"
     CDS             complement(9846..9920)
                     /locus_tag="PLA01_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HPC99781.1"
                     /translation="MISSSCACLFFFYQPKVPKALLHS"
     gene            complement(10105..10944)
                     /locus_tag="PLA01_00350"
     CDS             complement(10105..10944)
                     /locus_tag="PLA01_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99782.1"
                     /translation="MQGMSFLKFFLVSFPEQLLFVFLGSFAIGKYSYFKIRSNYYRVL
                     AVSLIMVGMSYLLKDKLGWGAESTLLILFSYIISIIFVMKFKFYESIAASIFAFSLLL
                     LVEIPVAVVIGSILRVDNDKAFTESTTLYFAIFFVIRLLQFLLAFLFYRFKFKVINME
                     YPNNMVNEYYIQLFVYLISICTLGFLAFLMARMLLIDTNTMVSTQNIYLLRLNIYITV
                     FVTIILSLAVKSVYVFYKNKNTLNNNEVIQSLEYIQSLLNEENYKEADEALSTLKEHI
                     IGQ"
     gene            complement(11047..11760)
                     /locus_tag="PLA01_00355"
     CDS             complement(11047..11760)
                     /locus_tag="PLA01_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPC99783.1"
                     /translation="MNNLGALFREIRLSKKWSIRQAAKKMGISYSYLSILEKGVDPRT
                     GKDSNPKPETLKIISKAYGYPYEELMKAAGYLSDDNSIPKKFSLDIFVNNLKLLIENM
                     TIEEFSRDIYQKTGYQIKPSQIKSYLKGDIEPFPGTLHILSKYAEVSMDFWYTNNSKD
                     TLEAEREKSRQNKIKSYQEPFSKEYAVFYNLQSDIKRFLSSEENIPYIKVAMEAQKKK
                     IKPKTLKLLVDTIVNEKEA"
     gene            12213..12485
                     /locus_tag="PLA01_00360"
     CDS             12213..12485
                     /locus_tag="PLA01_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99784.1"
                     /translation="MEHLKKRLLEESLRFIELCQAYYLKGRIDGSTYNSLTGIKLHFI
                     KDVLKRERIETCCEKKLLQKVDSLFMMDKKISNSDKIVVCLKDCCV"
     gene            12597..13820
                     /locus_tag="PLA01_00365"
     CDS             12597..13820
                     /locus_tag="PLA01_00365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPC99785.1"
                     /translation="MKYKNKDCFILGVIILISFIIKLVLIFIYQNRLTLASDDLNYVK
                     SAVVLIKRGIYTFHNLNEPTVFVTPTYPFFLALVFRVFGYSLRGMQAVRIIQAVFSCV
                     TILLTYLIAKELFNERVGLLASFFVAFYIPNIITVGFVLTETLFTMLLCLLIYLSLSF
                     AKRPNNARSAVLGVLWAVITLCRPTIALYPVMLFIYLFWEKRLKIVEMAKMGGIMFTT
                     FSVIMSPWWVRNYMEYGEFIPLAASSGNPFLQGTYVNYEQTPENIVYYKLGKNAFETN
                     KTETEVAKMRIKQEFKRDFARYLKWYTIDKAWMLWGTVFYWKRFFKIPSEYVIFCHYV
                     LIYSALCGTLILLFKNLKKYSLIMMLLLYYTAVHCVYMAFDRYAFPLIPLVTIISSYF
                     FITVINAGKRLVFAK"
     gene            14027..15007
                     /locus_tag="PLA01_00370"
     CDS             14027..15007
                     /locus_tag="PLA01_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HPC99786.1"
                     /translation="MQDIEKEISRLYDLRVERIYPVKSYYVIETPEGNKVLKNMNFSP
                     ERIMFVHGAKEHLYNNGFKYTDRYLCTKDEKPFFLINGETYTVTEEVIGRECDLNKTE
                     DVIRASKTLALLHKASKGYVPPRNCLVRDDLGNMPVYFNKRLEEIKRAKKIAQREKGK
                     FDYLILRYIDYFYELGQDAVNKIDRSGYKEVVDRCRKEKSFCHHDYSHCNIICGERET
                     SVVNFDFCSFEIKVYDVANLLRRKMRKCNWDVKGAKIIIDAYTSVEPITREEFDVMKI
                     MLQFPQKFWRVINKYYNSRRSKREKSFMCRFNEVIEEIEHQRKFLKEFGL"
BASE COUNT         4767 a         2593 c         3008 g         4696 t
ORIGIN      
        1 tttttgacgg atgggcatta acaatctccc gggcgttttt ggtaacatcc tttggggtat
       61 attcatccat cataataacc agatccgcca catcaaagta atctccggaa ccgccaacaa
      121 ctataattgt cgatactccc ttttccttat aaagctgacg gaccttgtca ataaaagggg
      181 ttataggctc cctgtctttt gaaacgagct tttgcatcct ttcatcacgg atcataaaat
      241 tggtggctga ggtatcttcg tctataagca aaagatccgt accaatttcc aatgcctcaa
      301 ttatgtttgc cgcctgtgat gtacttccgc tggcgttttc tgtggaaaaa atcttagtat
      361 cctgtccgtt gggaaggttg tttataaagg gtgaaatatt aaccttttca acccctcttc
      421 cgtcttcggc acgtatcttt accgcatccg tgcttgctgc cacgtattcc cttccatccc
      481 ctgaaatatg gttgtataca cccagctcca gtgcgtgcag cagggtcgat ttaccgtggt
      541 aacctcctcc gacaatcaaa gtaaccccct caggtattcc cattcccttt acaatgccct
      601 tgtggggaag attaatttcc acctcaaggc tttcgggact tttgaagggt attgcaccct
      661 ttgaaagtgg cctgtcagaa ataccgctct ctcttggcag aacagaaccg ttggctataa
      721 atgccacaag accctttgcc ttgatttgct ccctcaagaa ggcctggtct tcacacaaat
      781 ccacttgctt ttttaaggca ttggcatcga tattgctgta aaagagagtt ttttcaacta
      841 tctgtggaat agcaccggaa aaaatcgtat ccgcatattt tcccagtacc cttcttccgt
      901 tggcaggcaa tccgacttca aatcttactt ccaccttatc ttcgttaatt actgccgatg
      961 tcctgtctaa aatctcctgg ccgcagcggt caatagagat caacccgctt ttacctgaac
     1021 caccggatgg gttgtaatat ttttttatat tgttaaaaaa aagccgggtt aaaaaatcaa
     1081 ttacccctat tcttttatgg ggcttgtcat acaaagtgcc gggaaaacct gcatcctttt
     1141 gagacacaat aactcttacc tttgaaggcg atgcaaaagg atctccctga acatgatcaa
     1201 ttgccaatat atatctatcg aacttatact ctccctttat gtccttataa gccttgtatc
     1261 ccgctccgtc tatggaaaga agttttcttt gcaaatctgt tttatccttc atattatatc
     1321 ctccaataaa agctttccct gaaatagtaa cacatctttc ttaagattta aagcagcaca
     1381 taaaatagag gccaataaaa aagtgaagta caaatataaa agaaacttat gtttcttcta
     1441 tatttttctc cacctttata aacagatatt tattaacttg aaatttacca ttttaaaatg
     1501 cactagaaaa caaaaaactt atggtatgca ctgaaagaaa taagaataat actggacaaa
     1561 tcgactaaga aaaccaacaa ttggggtacc gtgtatttga attcttgatt ttcatcgaac
     1621 ttcaagttgt gtataatgct tccgagccaa tagacaatta caaaggcaca taaaaactta
     1681 agccacaatt gattcaaata aaatgtgcag taaacgaaaa atactattac taatatagat
     1741 ttagcattgt tatataacac tctgaactta tttaagtcaa aacttataag atcctcgatt
     1801 tcatctaaaa ctcccggttc ggattcatcg aattccatat ccgtgccgaa taggatgtta
     1861 tatagtgatt ttaatgcagc aaatactaaa atcactcctg atactataaa aaacaaggta
     1921 tcgttagaag caaccaataa taccggtatc aatattaacg ttagaataaa cattagcatg
     1981 ctttcaattg acattaatta tctcctgttt cttcttgatt aaaattaact gtaagtgcgt
     2041 tgtatactat taccgacatc tcaccccggg tcagtttatg gttaggatca agactgagat
     2101 tggttgtcag attaatttcc gttgctttat ccattacgtt gtttggccat tcaccactta
     2161 aggttttttc atatccaagg gcacgaatta cgactgctaa agcttctgca taggttacgt
     2221 cattatccgg tgcaatggtg ttattgggat atccggcaac caggttgtac tttattgaca
     2281 gcttgatgtt gttataagcc cagtgtgtgc tgctgatatc ggtaaaagta accttaacat
     2341 tatctatatc ggcatcagtg tcatatccca ttagcttaac taccaaagtg ataaattcac
     2401 ttcttcttat cttgttttca agccttaaag acccgtcttc ataacccttt aaaataccca
     2461 gagctttaag cgtatttgca ttatcctgct gcagctgagg tatgtcttca gcagcactgg
     2521 ctgtggggat tatggaaaat aatgcagtga taataaccaa taacacactt atcatttgtt
     2581 ttttcatcta aatcaccttt cctgttacta actagattat atatacctaa attatacacc
     2641 catattcaat tgtttaatat taaataacaa ttacaattag tatcacgttt tttaatgaat
     2701 ttcaagcatt tccccataat attttttatt attgaaaaaa gtagtatgac atgcttcctt
     2761 ctattaatga ataattgagg cgacattaac taaaactaat tgtgattaaa tgaaggaggt
     2821 ttttttatgg caaaaactat tgctgcaatt tttgataatt ataataatgc acaaagggct
     2881 gcacaacaga taaaggatca gggacttaag accgatgaca tttcaatagt tacaaaacag
     2941 ggtgatgaaa cagatgccgg aaacaactat tctatgggga taggaaacat gggcggaaat
     3001 atgactgcca ataataccgg agaaaacaac caggggggtg ttaatgataa catttccaat
     3061 ggagttgtta cgggcggtat tcttggagga ctggccggtc ttttgattgg tgcgggcagc
     3121 atggtcattc cgggattggg tattattgct gcagccggtc cgataaccgg gcttctgtcg
     3181 ggagcgataa ccggaggagt ggtcggcgga ttggttgacc tggggattcc ggaggacaaa
     3241 agcagacagt atgaaaccga cattaaagcg ggaaagactc tttttagtat gaaagtggat
     3301 gatgacaaga tagaagaggt aagttcaata ttgagaaaca acggtgcagc aagtgtagac
     3361 agctactaaa ggaaatttta gaagcgagac gggaaaatcc gcctcgcttt aatctaattt
     3421 gatgaatgca attattaagc ggagaataat atggaaggaa tttaagttac ttctattatt
     3481 actctccgct tccttttaaa gattggaatg gagcaaaaga aacaatttat tgaaacaggg
     3541 tttcatccac aacttcatat gaaggcccgg atgccggttg cggcacggga gttgcggtaa
     3601 cctcaacctt ttttgttccc ctctttatta ttgtcgtgta aggagaataa gtgcttctgt
     3661 gaagcttggt tttgctgata acagtaccgt ttatttttac cgttttgtaa gtatccacaa
     3721 catagcctga atgcccggat tgaactacaa ccgtttgacc ttctgccagg ctcggatcgt
     3781 ctatatattg tataggtgcc gggctggagc ttatttgtac gtgactcagt tcaatcgtct
     3841 tgccgggctc ttcttgtgtt ccgtaaatag taaaggttat tgtaccgctt tttacgcttg
     3901 attcaatttt tatcggccag ttggaattgt tcttgaactt aaaatccagg tcattgtacg
     3961 atacggccgc atccctgccc aaaggcagat aacttacgga aaacatgtga ttctgtctcg
     4021 ataccgtttc aaggtctgca aacagcacgg cattgtaaag ggttgaagaa acctggcaga
     4081 ctcctccgcc gattccgtca actatttttc cgccgacata ttcttttgct gtcttgtatc
     4141 cgttttgaac cgttcttggt ccgacaactt cattaaacga aaatacttca ccggggccaa
     4201 ggattttgcc gtttattttg gaggcagcaa cggatatgtt tatacccctg ttatagttgc
     4261 tcgtacctga agtggaaaaa tgcgtgctgg cactggaaag cttatcctta aagagcaggg
     4321 aattaacttc agtactattt atttcgggtt gttcaaaggt aaccggaaga attatttcag
     4381 catcagtttt atgtgcatgt tcgtttatta aactcagcaa ctcggattta tcgattttgc
     4441 gtccgggaac atggggtatt acaacgactt tgttgtcttc tacggttgct tttgcattac
     4501 tgggctcgca tacaatttta ttgtatatct catcggcatc aatgctcttt ggcgtgattg
     4561 ttatggaagg tatgtcaaaa attccgcctg tgcatgactt gattgagttt tctattatct
     4621 catatgcttt ttcggtgtca atggattcac cgggatgtcc ggtttggagc attacttttg
     4681 actcctcaat caatacatct gtttccttta cgggggttaa tgatttggaa taaaaactgt
     4741 caattacact ttttacgctg ttttcatcaa aagaataatc taaatcgatg ttcaagccat
     4801 ttagcttggt accgattatg ttggccagtc tttgaaatat gtttcctttt ctgccgactt
     4861 cgaaggcttt ttgtgcagct ccctcaatat catactgtac atttaattgt gataggctaa
     4921 attcctcaga aatatcacct ttcttaagaa caaccttcgt atcttttaat ttttcggtaa
     4981 agtttgagtt caggtgattg ttaatatcct cgtaagacat tttacttaca tcaataccgt
     5041 taataaatat ccccacatgt gccttgtcat aggacaaagt gaaataaaca gcaaatgaaa
     5101 acacaaccaa tagaacagct aataatatca ataaagaaat taatattatc cgggctcttg
     5161 agttgaagct tttgttttgg atcactgtat cagtcatacg aacactccat atccacatta
     5221 atatttatat taatagacgt attactatat tgttttgttc atcaaaaagt tatattaaat
     5281 tatgctaaaa tatatatcat tatagtcttt acgggtttag ctacaattct attatcgcaa
     5341 ttctaaagaa aaaaaacaat atatttttac atgtaaatat gtaactttgg gttaatgcaa
     5401 atgtatatat ataatgatgc gtcataaagc agcagacggt aaaaaaatgt atagccggga
     5461 aaaatcatgt tcatactttt atatgtatgc acaagattta ccgcaataga atatataatg
     5521 aataaaaatg ttctaaaggc tatgaaaaaa acattttata tcagcagagt ttcaaaaagt
     5581 gaaatagccg gactaatacc aaaattataa tatagggtga taaaaatgga cttaaacgca
     5641 cagaaaactc aatccgttaa actgctgaaa aattctatat tttcaaaaat aaacagtctg
     5701 ttgaaagaga aaagtgagac aatttatacc tataataccg gtgagaatga ggacctcata
     5761 gaaagcatta atgatgctaa aaaagaatgg ttaagtgcca ggaacaattt tgagcaggct
     5821 gttgagagtg aaattattga ttattacata tataagatta aggcttatca gataaggtac
     5881 gagtatcttt tgaaaaaggc aaaagaaaaa ggtattagtg tctaaccttt gtttttcact
     5941 cactcctttt tgtaataaat tcttgaacat gcctatatat ttaatatgaa aggattttta
     6001 accgcgtaat gatataagca tacttgttct aatcaaaata caaaggtgaa aacggagtga
     6061 aattggcatg tttcaaggat atcaaacagt atttgcctat attataggaa taatactttt
     6121 atattttcta ggaaggtttt taacatcacc tatgaaaatt gccgtcagac ttatacttaa
     6181 ttctttgcct ggtgttgtgt ccttattgct tgtaaacctt gcagggagtt ttcttagcta
     6241 tcatattgcc ttaaatcctg taactgtttt tattgccggc attttgggag tacccggggt
     6301 tttgctgctg gtggttttga aacaagtttt tcaggtataa ggtataaaaa aaccagcctc
     6361 tcgttccaca ttataaaaat ttaataaaaa caataaaaaa ttgtatacaa tgaacataga
     6421 ggtcgtaatt cgttgcagat gcgaagcggg cttatataag ttttcttgac aaaaaataaa
     6481 caaacattta tactttaatc tgtattagtt tatgttgtta taatataaag ctgtgttata
     6541 ttaaacataa taaaactgtt gaactgtgtt attcacaggt tctatatatt tttaatcaat
     6601 tgaggagggt taccaaatgg gcaaggttgt cgaaatcaga tggcatggtc gcggtggaca
     6661 aggtgctaaa actgcatcac tgcttttagc tgatgctgca ttcaacaccg ggaaatacat
     6721 tcaaggtttt cccgaatacg gtcctgaaag aatgggtgca cctattacgg cctataacag
     6781 gataagtgac gaaaggcttt ccattcacag taatatttat gagcctgatt atgtggtagt
     6841 ggttgatgat actctattgg catcggtaga tgttacagca gggcttaaag aagatggggc
     6901 tattattatt aatacttcca agtccccgga agaagtcaga ccgcttttaa agggttacaa
     6961 aggaaaggtt tgtaccattg atgcaaaaac tatttccata gaaaccttag gaaaatattt
     7021 cccgaataca cctatgcttg gtgctgttgt aaaagtgagc agagtaatgg atgaaaaaga
     7081 attcctggaa gatatggtgg gttcatttaa gcataaattt gctagtaaac ctgaggttat
     7141 tgaaggaaat ataaaggctc ttgaaaggtc actgcaggag gtgaaaggtt tatgagcaaa
     7201 aaggaattaa aaacagttaa gcccgatgtt aattggagag aaataacacc gggtggagta
     7261 attgattctt ccggaaatgc acatcttttt aaaaccggtg attggcgttc catgaagcct
     7321 gtctggaata cagaaaagtg caaacagtgt cttctctgtc atcctgtttg tcccgattcg
     7381 tcaatattgg taatagatgg taagatgtcg gatattgatt atgatcactg caagggttgt
     7441 ggcgtatgta caaaggtttg tcctttcaaa gccatagact ttgtggaaga agcgtagagg
     7501 aggggaaatg aaatggctat tagagagaga ttaagcggta atgaagcgac agcaatcgct
     7561 atgaagcaga taaatcccga tgtggttgca gcgtttccga taacaccgtc tacagaggtt
     7621 cctcagtatt tttcagcttt tgttgcggac ggtactgttg atacggagtt tgttgcagtt
     7681 gaatcggagc atagtgcaat gtcagcatgt ataggtgcac aggctgcagg tggaagagca
     7741 atgactgcta catctgccaa tggattggca cttatgtggg aacttttgta tatagcagca
     7801 agttcaaggc tgccgattgt tatggcagca ataaacaggg cactttccgg gcctattaat
     7861 attcataatg accacagtga caccatgggt gcaagggatt ccggctggat acagctgttc
     7921 agtgaaacca accaggaagc gtatgataac atgcttatgg ctcacaggat tgctgaaaat
     7981 cctgatgtaa tgctgcctct tatggtttgt caggatggat ttattacatc tcatgcgata
     8041 gaaaacatag agcttttgga agacgataag gttaaggcct ttgtgggtga gtataaacct
     8101 cagtactatc ctttaaataa agaaaccccg attgctgtag gaccgttgga tttacagccc
     8161 cattattttg aacacaagaa acaacaggct gaagcaatga aaaatgcaaa gaaggttatt
     8221 ttggaggttt cagaagagtt ctataagctg acaggcagaa aatacggttt gtttgaagaa
     8281 tacaaaaccg atgatgccga tgttgtagtt gttttattga actctaccgc cggtactgca
     8341 aagtatgttg cggatcagtt cagggcacag ggcaaaaagg ttggcgttat aaagccaagg
     8401 gtatacagac ctttccctgt ggatgaaata gcagaagctt tatcaaagtt taaggcagtg
     8461 gctgttctcg ataaagcaga cagctttaat gcagcaggag gaccattatt taccgatgta
     8521 acaagtgctc tttattcaaa gggagtattc ggtcctaagg taataaacta tatttacggt
     8581 ttgggcggaa gagacgttaa ggctgatgat atagaggttg tgtacaataa attgttagat
     8641 atcgcaaaga caggtaaagt agaatcggtt tataattacc tgggcgttag agaataggag
     8701 gtgcggtaaa atggcttata atgtaaaaga ggcagctcaa aagcccgaca gacttaccgg
     8761 cgggcacagg atgtgtgcag gttgtggagc acctgtagtt gtgagaaatg tgcttaaggc
     8821 attaaaaccg gaagatcatg cagttatagg cagtgcaacc ggttgcttgg aagtttcaac
     8881 atttatttat ccctatacag catggaagga ttcctttatt cataatgcat ttgaaaatgc
     8941 cgctgctaca gtatccggag cggaggcagc gtatgtggca ttgaagagga aaggaaaact
     9001 tgatggagat actaagttca tcgcatttgg cggtgacggc ggaacatacg acattggatt
     9061 gcagtcattg tctggtgcta tggaaagagg gcatgacatg gtgtatgttt gctatgacaa
     9121 tggagcatac atgaacacag gtatccaaag gtcttcggct actcctaaat atgctgacac
     9181 tacaacttca cctgccggga agaaaattcc gggtaaattg cagcctagaa aagatcttac
     9241 agaggttatg gcaaatcatc atctgcccta tgtggcacag actgctttga tcggaaatat
     9301 gaaggatctt tatgaaaagt ccgaaaaggc aatttatact ccgggaccgg catttttgaa
     9361 tgtattggcc ccatgtccga gaggctggag gtataatact ccggacctta tggaagtaag
     9421 caagatggct gttgagacat gtttctggcc tctttatgag gttgttgacg gcaagtataa
     9481 cattacctac aaaccgaaga acaagctgcc ggtaagagat ttcctgaaga tgcagggaag
     9541 gtttaagcat ctcttcaagg ccggtaatga gcatatgctt gaagaaatac agcaggaagt
     9601 agacagacgg tgggaggcac ttttaaggtt ggccggtgaa gcttaagttt tgtatggatt
     9661 ttttaataaa agcggagggg agttaaactc cctttcgtta tattagtggg atttcacgaa
     9721 gtgaaatgac caaaaagcga tgggggctcg atgcccccgg agcttttgtc cattgataat
     9781 aaaactgccg gaaaggttta acggaagaaa cctttctgac agttttttcg tttcaaatta
     9841 tgcggttagc tatgcagtag tgcttttggg actttaggct gataaaagaa aaacaaacag
     9901 gcacagctcg aagaaatcat tgcgataaca aaaagagcaa cagtgattga actgagaatt
     9961 tttctataca tgttttttcc ctccctacga aattttttat accagttata taggcctatg
    10021 cacggtatct gtgtcaggtg tggttttttc tgtctatcct tattatattc gtatatattg
    10081 aactcctatg ttttttatta aaggttattg cccgataatg tgctctttta aggtgctcaa
    10141 tgcttcatca gcttccttgt agttttcttc attaagaaga ctttgtatgt attctaggct
    10201 ctggataacc tcgttgttgt taagggtatt tttattctta taaaaaacgt aaacactttt
    10261 tactgccaga gataaaataa tggttacaaa tacggtgata tatatgttta accttaaaag
    10321 atatatattc tgtgtcgaaa ccattgtatt tgtatcgatg agcagcatcc tcgccattaa
    10381 aaaagccaga aacccgaggg tacatatgga tatcaaataa acaaacaatt gtatatagta
    10441 ctcattgacc atgttattcg gatattccat gtttattacc ttgaatttaa agcggtagaa
    10501 aaggaatgcc aaaaggaact gcagtaaacg tattacaaaa aatattgcaa agtatagcgt
    10561 ggtgctttcc gtaaaggcct tgtcgttgtc tactctcaaa atagaaccta ttacaactgc
    10621 tacgggtatt tcaaccagta ataacaacga aaatgcgaat attgatgcgg ctatggattc
    10681 ataaaacttg aatttcatta caaatataat tgaaataatg tacgaaaaca atataagtaa
    10741 cgtgctttct gctccccaac ccagtttatc ttttaaaaga taactcatgc cgaccattat
    10801 caaagaaacg gcaagaaccc tgtagtagtt tgagcgaatc ttaaagtaac tgtactttcc
    10861 gatcgcgaaa gagcctaaaa aaacaaataa caactgctcc ggaaagctga ccaaaaaaaa
    10921 ctttaaaaat gacataccct gcataataac cacacccctg aaatcttttt aaatatagca
    10981 aacagtttaa tatgagtcaa gctatttatg gaatatttcc ctgtaaatac tagtttacct
    11041 gattaattaa gcttcctttt catttacaat ggtgtccacc aatagtttta aagtttttgg
    11101 ttttattttt ttcttttgtg cttccatggc tactttaatg taaggaatat tttcttctga
    11161 agacagaaag cgctttatat cgctttgaag attatagaag acagcatatt cttttgaaaa
    11221 gggttcctga tacgatttta tcttgttttg ccggcttttt tctctttctg cctcaagagt
    11281 atctttgctg ttgttagtgt accagaagtc catagaaacc tctgcgtatt ttgatagaat
    11341 atgcagggtt ccgggaaaag gttcaatatc gcctttaaga tagctcttta tttgagaagg
    11401 ctttatctga taccctgttt tttgatatat gtcccgggaa aactcttcga tagtcatgtt
    11461 ttcaattaat agttttaaat tgttcacaaa aatatccaaa gagaatttct tgggtatact
    11521 gttatcatca ctcaagtacc ctgctgcctt cattagctct tcataaggat agccatatgc
    11581 ctttgaaata atcttcaatg tttccggctt tgggttggaa tccttacctg tacggggatc
    11641 aactcccttt tcaagaatac ttaaatatga ataactgatt cccatctttt ttgctgcctg
    11701 ccgaatagac cactttttac tcaacctaat ttctcgaaat agtgcaccta aattattcat
    11761 gttatccacc ttgagtcaaa taatattcat aaaaaacgaa tataaatata tatataatat
    11821 gacataatat taatacaaat ggatgaaatt ttcaaaggga aatatttatt tttttacaac
    11881 caattattga caaggtccaa tttatagtat aacattacga gggataggtt gtttgtcacc
    11941 gtaatttcag attatttaat tttaaggcag ggaataatta ttatactggt agtgaaatat
    12001 tgaatttatg ttattgtttg tacacaaagt ggtaaattaa atgtgaatta ttatatataa
    12061 aaaagggtat taaatcaaaa aaaccaaaaa gcaactggat ttataatgtg ataaaaaata
    12121 aaaaacgtac aaacagttga cggaatttac tggtattaat tggggggaga ttattcgctt
    12181 aaaataatac ttctgaaagg gtgagcttca aaatggaaca tttaaaaaag agattactgg
    12241 aagaatccct tcgcttcatt gaattgtgtc aggcttatta tttgaaaggg aggatagatg
    12301 gaagtactta taattctctt acaggtatta agctgcattt tataaaggat gtactgaaaa
    12361 gagagagaat cgaaacatgt tgcgaaaaaa aattattaca gaaggttgat tctcttttta
    12421 tgatggataa aaaaatcagt aactctgaca agatagttgt ttgccttaag gactgttgcg
    12481 tttaaataat gtattaaacg gataaaataa tatcatacat tataacagaa taaaataaca
    12541 gaataaaatc agctgtgttc aaagagctat atataatgca tacggagtat aaaaaaatga
    12601 agtataaaaa caaagattgc tttatcttgg gagtaattat tctgatatcc tttattataa
    12661 aacttgtatt gatttttata tatcaaaaca gattaactct tgcaagcgat gatctaaact
    12721 atgtcaaaag tgctgtagtg cttataaaaa gaggcattta tacattccac aatttaaacg
    12781 aaccaacggt gtttgtaaca ccaacctacc cttttttcct tgcattggta tttcgggtgt
    12841 ttggctatag tttacggggc atgcaggcag taagaattat acaggcggta tttagctgtg
    12901 taacgatact gcttacatat cttattgcaa aggaactttt taacgaaagg gtaggattat
    12961 tggcttcctt ttttgttgcc ttttatatcc ccaatataat aaccgttggt tttgtactta
    13021 cagaaaccct ttttacgatg cttttgtgcc tgttgattta tttaagcctc tcttttgcaa
    13081 aaagacctaa taatgcccgt tccgcagtct taggagtcct ctgggctgta attacgttat
    13141 gcaggccaac cattgccctg tatccggtaa tgcttttcat ttatctgttt tgggagaaac
    13201 gtttgaagat tgttgaaatg gccaagatgg gaggcattat gtttactaca ttttctgtta
    13261 taatgtcacc ctggtgggtt agaaactaca tggagtacgg agaatttata ccccttgccg
    13321 catcaagcgg aaaccctttt ctccagggta cctatgttaa ctacgaacaa acgccggaaa
    13381 atatagtata ttacaaactt gggaaaaacg cctttgagac aaacaagaca gagacagaag
    13441 tagcaaaaat gaggataaag caagagttta aaagggattt cgcaagatac cttaaatggt
    13501 ataccataga caaggcatgg atgctatggg gaacggtatt ctactggaaa cggtttttca
    13561 aaatcccatc ggagtatgta atattttgtc attatgtttt aatctattcg gccctttgcg
    13621 gcacattgat tttactcttt aaaaacctta agaaatacag tctcattatg atgcttcttc
    13681 tgtattacac tgctgtacac tgtgtgtaca tggcttttga caggtatgca tttcctctta
    13741 ttccgctggt aacaattatt tcttcatatt ttttcataac ggtgataaac gctgggaaga
    13801 gattggtttt tgctaagtaa atggaagaaa tgttgtgaat tttcttcaca tttatttgtt
    13861 gcaccctgtt ctaatttttc ggacgtgtaa atattataac tattatgtat gtaataaatt
    13921 cgtttcgtta aaaagcattt aataatgctt aagattgttg aatgcatatt cgggatattt
    13981 tctgcattca aataaagcaa agatagtata tgggggttac attaaaatgc aggatataga
    14041 gaaggaaata tcaaggctgt acgatttgcg tgtagaaaga atttatccgg taaaaagtta
    14101 ttatgttatt gaaacacctg agggtaacaa ggtcttaaaa aatatgaatt tctcaccgga
    14161 acgtataatg ttcgtacacg gtgcaaaaga acatttatat aacaacgggt ttaaatatac
    14221 agacagatat ttatgcacaa aggacgagaa accgtttttt cttataaacg gcgagactta
    14281 tactgtcaca gaggaggtaa taggacggga atgcgacctt aacaaaacgg aggatgtaat
    14341 aagggcgtca aaaacactgg cattactgca caaggcctca aaaggatatg tacctccccg
    14401 aaactgcttg gtaagagacg atcttggcaa tatgcctgtt tattttaaca aaaggcttga
    14461 ggaaataaaa cgtgcaaaaa agattgcaca aagagagaag ggcaaatttg actatctaat
    14521 actcaggtat attgattatt tttacgaact ggggcaggat gcggttaata aaattgaccg
    14581 ttccggatac aaagaagttg ttgacaggtg cagaaaagaa aaatcctttt gccatcatga
    14641 ttacagccac tgcaatataa tatgcggcga acgggagaca tcggttgtaa actttgattt
    14701 ctgcagcttt gaaataaagg tttatgatgt tgcaaacctg ttgagaagaa agatgagaaa
    14761 atgcaattgg gatgtaaagg gggcaaaaat cataattgat gcatacacat ctgttgagcc
    14821 cattacccgg gaagaatttg atgttatgaa aataatgctg cagttcccgc aaaaattttg
    14881 gagagtaata aacaaatact acaacagcag gcggagcaaa agggaaaaaa gttttatgtg
    14941 caggtttaat gaggtgattg aagaaattga gcaccagaga aaatttctga aggagtttgg
    15001 gttgtaaaaa cacaaagacg gagcttttaa atgctccgtc tttgtattag aggctgaata
    15061 aaaa
//
LOCUS       DAOOJC010000006        13972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_4153_length_13972_cov_3.573256, whole genome shotgun sequence.
ACCESSION   DAOOJC010000006 DAOOJC010000000
VERSION     DAOOJC010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 13972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13972
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4153_length_13972_cov_3.573256"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PLA01_00375"
     CDS             <1..531
                     /locus_tag="PLA01_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HPC99787.1"
                     /translation="KGVHRLVRISPFDASGRRHTSFASLDVMPELSDDIEININPDDL
                     RVDTYRSSGAGGQHVNKTESAVRITHIPTGIVVACQNERSQIQNRETAMKMLKSKLFE
                     LKEREQKEKIEDLKGIQKEIAWGSQIRSYVFCPYTLVKDHRTNCEEINVESVMDGELD
                     RFINSYLSSSSNEKDQ"
     gene            complement(634..3570)
                     /locus_tag="PLA01_00380"
     CDS             complement(634..3570)
                     /locus_tag="PLA01_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009523 - photosystem II [Evidence IEA];
                     GO_component: GO:0009654 - photosystem II oxygen evolving
                     complex [Evidence IEA];
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HPC99788.1"
                     /translation="MLWIIMILSFTCVAYADGNINLNYNGRSYVIQKDSLITNNGEYY
                     FDAYKTAEVLGLKLVFDSQHKILTVHNGESASTYYIAPLDYSIVSLKSTRPNTPELIN
                     NSIYFPVSFIEEKFNIIVKYDKKSDSVYFLPDKNLKTFKNMKHSYTLTIPDYVNFDLS
                     GPCDSFSEDSIVLSDKNQEFTYSINCDRLDILSIAGMRMILNDYDSTDEQIFDQINIY
                     TKSYFRAMQDLYKSEFLFSGTDTLLSESNIKIFADTNESIHGQPSDLILYNTEKTDRY
                     SSLEETHIMITVPIYSKLSIYTFNIYGKKGFLREENINKILGLINALKIEGLPNTSTT
                     LKTLADNKIVSAANLGIYPPLAKTASFYTEYNNSGQNYSIRYPSVFLPYLQNQIIDSL
                     DYTSFKMDYNNYFSISVENTQNPAVAIKEKTDLLKKSINNRMTVKEEGNVILSGKEFY
                     RLSYETYNGPDPYYVLSYYTVNGSKLYTIELNSRFIRPSAEITNAFLEIVKSIQFTEP
                     TKAEQTLNINFKKYLNDYEGYSFSYPENWNLSDISNDINFERLFMHIPEYSGAVEICV
                     NESECLIDASPEELLEMLVSGKANDFKKYTKNYYAPYSDRASKVVNISSRIENNAVYA
                     YRLINYLDESDRHKIGYCVDVIRNSKLYTLFISISDYLSTDGNLLDKSLDYIVNQIAQ
                     SFTLEESGKLLFKAESGSQKGRKLTFLEHSFKLVMGKSTTIPYARYLDTNGDMLLYIS
                     NCKEAGAYKLKFDYDKKNLEITSTLSQENAVNNAKIKLLNMYNDKVIHSITPDLDNMT
                     LTIEYSNTVFSPISKKTYFIDVLPSKSGFYIQLVRKYTTDQLMNEIQSHMENYLMTKV
                     DIQLSDSNNYTNNILLTDKQEIQFISLFAQYNNRAGYFILRIDPYADNIRIYKFISFD
                     EVEEKLKEYYNSPYSDLSFVDYTIDDKNKFGLKVHLFSKSASTDVVHNLTVEFDEKTF
                     EIIFGS"
     gene            3826..4203
                     /locus_tag="PLA01_00385"
     CDS             3826..4203
                     /locus_tag="PLA01_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPC99789.1"
                     /translation="MDIVISNSSEKPIYEQITTQIKDAIIKGVLKEAEILPSIRSLAK
                     ELKISVITTKRAYDELEREGFISIVPGKGTFVAAQNKDLLRESRIRIVEEKLIEAVEA
                     AKSIGLSFDELLEMLKLLFEEDD"
     gene            4205..5071
                     /locus_tag="PLA01_00390"
     CDS             4205..5071
                     /locus_tag="PLA01_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC99790.1"
                     /translation="MTAILEIKNLRKEYKDFSLKDINLTLEKGFIMGFIGPNGAGKST
                     TIKLILNLVNKDGGEINIFGKDNIAFEKEVKNRIGFVLDEGYYYEEITVREMKSIIAH
                     FYDRWDEGAFKKYAKDFGLPIEKKIRELSKGMKMKFSLAIALSHDAELLIMDEPTSGL
                     DPVVRSELLDILSDLMQDENKGVFFSTHITSDLDKIADYITFINNGEIIFSTTKDDIM
                     DNFGVVKGPKELIRNNGSNGFIGIRENQYGFEALVQNKEEFRKLYGDIAVIEKPTLED
                     IMVYYTRRGIYA"
     gene            5064..5720
                     /locus_tag="PLA01_00395"
     CDS             5064..5720
                     /locus_tag="PLA01_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HPC99791.1"
                     /translation="MLNLVIKDILVQKKIFIASILYIIFFVIAFQSLGMGMYTAAIVA
                     IVYLMVSGGFAYDDKSKSDIMLNSLPIKRENIVMSKYISLFVYIAIGTIAYIVVTFVI
                     SLLNTPIKTYPVTIELIVSAVIAVSLMHSISFPLIFKLGYVKSKAFNMIFFLSFFFGA
                     PLLIDFVSKANSEVTTAVWTFLKNQSDVAIVFELLVLSLVFLSISYGISVRLYKKREF
                     "
     gene            5949..6815
                     /locus_tag="PLA01_00400"
     CDS             5949..6815
                     /locus_tag="PLA01_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HPC99792.1"
                     /translation="MNIGIIGAGRVGCAFGIGLKNSGFSVAGVYSRSDESQRFLSSKL
                     ELNCINGLRETVKNSEVILISVPDNSIKDLAVEIIQKTEAEHVDSKTFIHLSGALTSE
                     VLEPLQKAGAFVGSLHPVQTFADRENGWEKLYNIYYGFEGCDKARDIALRIIKAFEGK
                     IIHIDKKDKPLYHMAACIISNYTVTLSHVAGKILENIGIDGEDAAKVFLPLIQNTVDN
                     IGKFGSAGALTGPISRGDYKVVEEHIKALIGLEPQISDIYKILGQKTIDLALIKGTLL
                     DDSADKLRSLLS"
     gene            6920..7666
                     /gene="larB"
                     /locus_tag="PLA01_00405"
     CDS             6920..7666
                     /gene="larB"
                     /locus_tag="PLA01_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HPC99793.1"
                     /translation="MDSEYLRKLLEKVKDGNVTVDEALLELKKLPFEDLGFVKVDHHR
                     NIRNGYPEVIYCQGKTAEQVKKIVEKLMESNNNIMATRADVKVYEAIKEITSDVVYHE
                     AARIVVVKKRDIPVSDKIVAVLTAGTSDIPVAEEAAVTCETMGNRVERVYDVGVAGIH
                     RLFARADIILNANVLIVVAGMEGALASVVSGLVDKPVIAVPTSVGYGANFGGLSALLS
                     MLNSCAAGIGVVNIDNGFGAGFLAAMINKL"
     gene            7683..8969
                     /gene="larC"
                     /locus_tag="PLA01_00410"
     CDS             7683..8969
                     /gene="larC"
                     /locus_tag="PLA01_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HPC99794.1"
                     /translation="MKLLYFDCFSGISGDMVLGALIDLGIDVDVFKSELQKLGLDEFD
                     ISVEKKMKNSITVTDVDVLIKDKVHDHVHSHGCEKEQNHDHGCEKGQHHNHGCENGPH
                     GRYHHHHASARNLADIEKIIDDSNLKDSVKQLSKKIFGEIARAEAKVHNKPVSDVHFH
                     EVGAVDSIVDIVGASICIDLLNVDRIYSSPLHDGTGFIECQHGKLPVPVPAVMEMLKE
                     SNIPYIVEDVNTELITPTGIGIIKTIASGFGTMPPMTVDKVGYGAGKRETGRLNALRC
                     VLGTTSDEGIKDNEIAVLETNIDDMNPEVLGYVMDLLFESGALEVYYTPVYMKKNRPG
                     VMLTVLSEKEDEKKLEDIILKETSTLGIRKTFATRYVMNRENRWVDSEFGKISVKEAY
                     YEGIKKFAPEFEDCRKAAQKHNVPLWKVYNAAIKQI"
     gene            9076..10008
                     /locus_tag="PLA01_00415"
     CDS             9076..10008
                     /locus_tag="PLA01_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512652.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPC99795.1"
                     /translation="MLYNKFSSYLKNRYGTKVYKLPLNLPVTCPNRDGRISDKGCIFC
                     GEEGAGFENLPNCLGIKQQLSLNSKYIGANYNSDKFIAYFQNYSNTYLPLSDFKIYIN
                     EALSENIVALYISTRPDCIKDSYLEFLKEIKASKGVDIVIELGLQTVNYHTLRFLNRG
                     HLLAEFIDAVLAVKSYGLESCAHYILDLPMDSMEDLIEGARILSALGVSQVKCHSLYI
                     LKGTTLGDMYERGEIRPLSLEEYIDRAVSFLEYLNPSIVVQRLIGRAPKERTLFCNWG
                     MSWWKIHDLIEKKMTEEGSYQGKRFDYLNGRALT"
     gene            complement(10333..11727)
                     /gene="gltA"
                     /locus_tag="PLA01_00420"
     CDS             complement(10333..11727)
                     /gene="gltA"
                     /locus_tag="PLA01_00420"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPC99796.1"
                     /translation="MPNMSLKKVPMPEQAPDVRNKNFLEVASGYTEQMAIEEAQRCLN
                     CKNKPCVQGCPVNVQIPEFISLVAQGKFEEAYNKIKETNSLPAICGRVCPQETQCEQR
                     CVRGIKGEPVAIGRLERFAADWHIKNAEDFVEKPNKSGKKVAIIGSGPAGLACAGDLA
                     KMGYDVTVFEAFHVPGGVLMYGIPEFRLPKELVQKEIDTIKKLGVEIKTNMVIGKVLT
                     LEELKEEGYEAIFIGSGAGLPSFMKIPGENLNGVYSSNEFLTRINLMKAYKFPDYDTP
                     VKIGKNVAVVGGGNVAMDAARCAKRLGAQNVYIVYRRSEAEMPARLEEVHHAKEEGII
                     FKLLTNPTRILGTEDGWVKGMECVEMELGEPDVSGRRRPVAKAGSEHVIEVETVIIAI
                     GQTPNPLITSATPGLDTQKWGGIIADEKTGSTSIEGVYAGGDAVTGAATVILAMGAGK
                     NAAKAIDEYLRSKE"
     gene            complement(11730..12548)
                     /locus_tag="PLA01_00425"
     CDS             complement(11730..12548)
                     /locus_tag="PLA01_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPC99797.1"
                     /translation="MFRIVKKEVLNPQVKLMEIDAPHIAKKAEPGQFIILRISENGER
                     IPLTIADYDRTQGTVTIIFQEVGKTTIALGELNEGDHILDFVGPLGKASHFEGIKKAA
                     VIGGGLGTAIAYPQAKKLNSMGVEVHSIIGFRNKDLIILEDKMKAVSSKLYITTDDGS
                     NGKKGFVTDVLKELIEEGNKYDLVIAIGPLVMMKFVSSLTKQYGIKTIVSMNPVMVDG
                     TGMCGGCRVTVGGETKFACVDGPDFDGHLVDFDEAIRRQNIYKSEEHSCRLGDR"
     gene            13063..>13972
                     /gene="gdhA"
                     /locus_tag="PLA01_00430"
     CDS             13063..>13972
                     /gene="gdhA"
                     /locus_tag="PLA01_00430"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPC99798.1"
                     /translation="MKLLSNIMEQVIKKNPNEQEFHQAVEEVLESLEVVAENNPEYLK
                     AGIFERIVEPERQIIFRVPWVDDNGKVQVNRGFRVQFNSAIGPYKGGLRFHPSVCLGI
                     IKFLGFEQIFKNSLTGLPMGGGKGGSDFDPKGKSNGEIMRFCQSFMTELCKHIGPDTD
                     VPAGDIGVGGREIGYMYGMYRKIRNEFTGTLTGKGLTWGGSLARTEATGYGLCYFMEE
                     AMKTIKGKSFKGTTVVISGSGNVAIYATEKAQQLGAKVVALSDSNGYIYDPDGIKLDT
                     VKQIKEVERKRIKEYVKHHPNAQYTEG"
BASE COUNT         4288 a         2441 c         2961 g         4282 t
ORIGIN      
        1 aaaggagttc accgtctggt gagaatatcg ccctttgatg cttcgggaag aagacatact
       61 tcctttgcat ctttggatgt tatgccggaa ttaagtgacg atattgagat caatattaat
      121 cctgatgacc tccgtgtaga tacttaccgt tcaagcggtg caggtggtca gcatgtcaat
      181 aagactgaat ccgctgtgag gataacccac attccgacag gtatagtggt tgcctgtcag
      241 aatgagcggt cacaaataca gaacagggaa actgctatga aaatgctgaa atcaaagctt
      301 tttgaactaa aagaacggga acagaaggaa aagatagagg atttaaaggg gattcagaag
      361 gaaatcgcat ggggaagtca gataaggtct tacgtttttt gtccctacac ccttgttaag
      421 gatcatagga caaattgtga ggagataaac gttgagtctg ttatggatgg agaattggac
      481 aggtttatca attcatacct ttcaagctct tcaaatgaaa aagatcaata aattaaaagc
      541 caagtaaaaa tataaggaaa tatggaagaa actgcagtgt ttcttccgta tttttatgaa
      601 cttaaataat atgcatccta aatgacatac accttatgaa ccgaatataa tctcaaaggt
      661 cttttcatca aactccaccg tcagattatg aaccacatcg gtggaagcgg attttgaaaa
      721 caggtggacc tttagtccaa acttattctt atcgtctatt gtatagtcta cgaaggacaa
      781 atctgaataa ggtgagttgt aatactcttt gagcttttct tccacctcat caaaggaaat
      841 aaacttgtat attcttatgt tatccgcata agggtctatt cttagaataa agtatccggc
      901 ccggttgttg tattgggcga atagactgat aaactgaatt tcctgcttat cagtcagtaa
      961 aatattgttt gtatagttat tgctgtcgga taattgtata tccactttgg tcatcaggta
     1021 attctccata tgggattgaa tctcattcat tagctgatcg gtggtatact ttcttacaag
     1081 ctgtatgtag aagccgcttt tagacggtaa cacatcaatg aagtaggttt ttttagatat
     1141 gggagagaaa acggtatttg agtattcgat tgttaatgtc atattatcga gatccggtgt
     1201 aatagaatga attactttat cgttatacat attaagaagt ttgattttgg cattattaac
     1261 agcattctct tgagaaaggg tacttgtgat ttcaagattc tttttgtcat agtcgaattt
     1321 tagcttgtat gctccggctt ccttgcaatt gctaatgtaa agcagcatat caccgttggt
     1381 atccaaatac cttgcgtagg gaatggttgt agattttccc ataactaatt taaaagagtg
     1441 ctccaggaat gtcagttttc tgcccttttg actcccggat tcggctttga aaagtaattt
     1501 gcctgactct tcaagggtaa aggattgtgc gatttggttt actatataat caaggctctt
     1561 gtctaaaaga tttccatcag tgcttaaata gtcgcttatg gatataaaca gggtatacaa
     1621 cttgctgttg cgtattacgt caacacaata gccaatctta tgccggtcac tttcgtccaa
     1681 ataatttatt aatctgtatg cgtaaacggc attgttttcg attcttgagc ttatgttaac
     1741 tacttttgag gctctgtcac tgtaaggtgc ataatagttt ttggtgtact tcttaaaatc
     1801 attggcttta ccgcttacta acatctccaa taattcctca ggagatgcat cgatcaagca
     1861 ttccgattca tttacacaaa tttcaacagc tccggagtat tccggaatat gcataaacaa
     1921 cctttcgaag tttatgtcat tggatatgtc ggaaagattc cagttttcgg gataagaaaa
     1981 ggaataacct tcatagtcat tcaagtattt tttgaaatta atgtttaaag tttgttcagc
     2041 ttttgtaggt tccgtgaact ggatggattt cacaatctcc aaaaacgcat ttgttatttc
     2101 cgcagaaggt cttataaatc tgctgttcag ttcaattgta taaagctttg aaccgttaac
     2161 tgtataatag ctgagaacat aatacggatc cgggccgttg taggtttcat aactcaagcg
     2221 gtagaattcc ttgccggaca gtattacgtt tccttcctct ttaacggtca tcctattatt
     2281 tatggatttc tttaacaggt cggttttttc ttttattgct acagcaggat tttgtgtgtt
     2341 ttccaccgat attgagaaat aattattgta atccatttta aagctggtat aatcaagact
     2401 gtcaatgatt tggttctgca aatacggcaa aaataccgac ggataacgta tactgtaatt
     2461 ttgaccggaa ttattatact cggtatagaa agaagcagtt tttgcaagag gaggataaat
     2521 gccaagattt gcagcactga cgattttatt gtcagccaat gtctttaaag tggtcgaagt
     2581 atttggcaat ccttcgattt ttaaggcatt tatcagtcca agaattttat tgatattttc
     2641 ttcccttaaa aatcctttct ttccgtatat gttgaaggtg tatatggata atttggagta
     2701 tataggcacg gttatcatta tatgtgtttc ttccaaagaa gaataacgat cagttttttc
     2761 agtattatat aaaatcaaat cggaaggttg accgtgaata ctttcattgg tgtcagcaaa
     2821 tatttttata tttgattcac tcagcaaagt atcggttccg ctgaacaaaa actcagattt
     2881 atacagatcc tgcatggctc taaaatagct ttttgtatag atgttaatct gatcgaagat
     2941 ttgctcatcg gtagaatcat aatcattgag aatcattctc attccggcaa tggacaagat
     3001 atcgagtcta tcgcaattaa tggaataagt gaattcttga tttttatctg acaatacgat
     3061 ggagtcttca ctaaaactgt cgcatggtcc cgaaaggtca aaatttacat agtcgggtat
     3121 agtcagagta tagctgtgtt tcatattttt gaaggtttta agatttttat cgggtaaaaa
     3181 ataaacagaa tccgattttt tatcatattt aacaattata ttaaacttct cttcaatgaa
     3241 gctaacagga aaataaatgg agttgttgat taattcaggg gtattgggac gtgtgctttt
     3301 caatgagaca atggagtagt ctaaaggtgc tatgtaatat gtactggccg attcaccgtt
     3361 atgaaccgta aggattttat gttgactgtc aaataccagc tttaatccaa gtacctccgc
     3421 cgtcttatag gcatcaaaat aatactcgcc gttatttgtg attaaactgt ctttttgaat
     3481 aacatagctt ctgccgttgt aattgaggtt gatattacca tcggcataag caacacaagt
     3541 aaagcttagt atcattatga tccataacat tattattaac aaataagatt ttttcatgtt
     3601 aaacactttc ctttaatata agttgttata aacagtttat attatgtata ttaccaaaag
     3661 ctttagccat atacaacaag tgttaactca ttgtaaaata agtgaaacat acatttaata
     3721 taccactctt ggaaagaaga aataaatatt gacaacggcc agtcatatga tatatactgt
     3781 atatatacgg catatacggt atatactcaa agaaaggagc aaaatatgga cattgttatt
     3841 tcaaactcct ctgagaaacc gatttatgag cagattacaa cgcagataaa ggatgctatt
     3901 attaaagggg ttcttaagga agctgaaata cttccatcca taagaagtct cgcaaaggag
     3961 ttaaaaatca gtgtaattac gaccaaaagg gcgtacgacg aactggaaag ggaaggcttt
     4021 atatccattg tgcccggtaa aggaactttt gttgcggcac aaaacaaaga cttgttaagg
     4081 gagtcaagga taagaattgt agaagaaaag ctaattgagg cggttgaagc tgcaaagtct
     4141 atagggctat cgtttgatga acttctggag atgttgaaac tgttatttga ggaggatgat
     4201 taaaatgaca gcgattcttg aaataaaaaa cctgagaaaa gagtataaag atttttcttt
     4261 aaaggatata aatcttactc ttgaaaaggg atttataatg ggctttattg gaccgaacgg
     4321 ggccggcaaa agtactacaa tcaagcttat attgaatctt gtgaacaaag acggcggaga
     4381 aataaatatt tttggaaaag acaatatagc atttgagaag gaagtaaaaa acaggatcgg
     4441 attcgttttg gacgagggtt attattatga ggagattact gtacgtgaaa tgaaaagcat
     4501 aattgctcat ttttatgacc ggtgggatga gggagccttt aaaaaatatg ctaaggattt
     4561 tgggcttccg atagagaaga aaattaggga gctatcaaag ggaatgaaga tgaaattttc
     4621 actggcaatt gcactttcac atgatgcaga acttttaata atggatgagc ctacatcggg
     4681 ccttgatcct gtggtcagaa gtgagctttt ggacatattg agcgatctga tgcaggatga
     4741 aaacaaagga gtatttttct cgacccatat tacttctgac ctggacaaaa ttgccgacta
     4801 tataacattt ataaataatg gagaaataat tttcagcaca accaaggatg acataatgga
     4861 caacttcgga gtggtaaagg gaccgaagga gttaattcga aacaatggct caaacgggtt
     4921 tatcggaata agggagaatc aatatggttt tgaagccctt gttcagaata aggaagaatt
     4981 cagaaaacta tatggtgata tagctgtgat tgaaaagcct acattggaag atattatggt
     5041 ttattataca aggaggggta tatatgctta atcttgtaat taaagatatc ctggtgcaaa
     5101 agaaaatatt tatcgcttca atactttata taatattttt tgttatcgca ttccaaagcc
     5161 tgggaatggg tatgtatacg gccgcaattg tcgctattgt ctatttaatg gtttcgggcg
     5221 gatttgctta tgacgataaa agcaaatcgg atattatgct gaacagtctt cccataaaga
     5281 gagaaaatat tgtaatgtca aagtatatat cattatttgt ctatattgct attggaacaa
     5341 tagcctatat cgttgtcact tttgttattt cactgttgaa tactcctatt aaaacctatc
     5401 ctgtcactat tgaattgata gtcagtgcgg ttattgccgt gagtttgatg cattcaatat
     5461 cttttccttt gattttcaaa ctgggttacg tcaaatcaaa ggctttcaac atgatatttt
     5521 tcctttcgtt tttctttggt gctcctttgc tgatcgactt tgtttcgaaa gcaaacagcg
     5581 aagttacaac ggctgtttgg acttttttaa aaaatcaatc cgatgtagct atagtttttg
     5641 aattgttggt tttatctctt gtatttctat caatatccta cggaatttcg gttaggctgt
     5701 ataagaaaag ggagttctaa atccgatacc cttgagtatt tgacgattaa tacacccact
     5761 cttgggtatt aataaaactt agaaattgtg atattattct attggtgcca tttctcaaat
     5821 ggacattggc aaattgttgg agcacttgat gatccgataa tttatagttt tcgtttcgta
     5881 agttgtaatt tagaagagag aatagaaacc tttgatttaa aaatcaatta acggggattg
     5941 gtgtgagtat gaatattggc ataatagggg caggaagagt gggctgtgct tttggtatcg
     6001 ggcttaaaaa cagcggattt tccgttgcgg gagtatacag tagaagtgac gagtcccaaa
     6061 gatttttaag cagcaagctg gaattaaact gtataaacgg cctaagagaa accgttaaaa
     6121 actctgaagt tattttaatt tcggtcccgg acaacagtat aaaagactta gccgttgaaa
     6181 taattcaaaa gacagaggcg gaacatgttg attccaaaac ctttatacat cttagcggtg
     6241 cattgacatc ggaagtattg gagccattgc aaaaggccgg tgcttttgtt ggctctctcc
     6301 atcccgttca gactttcgcg gacagggaga acggctggga aaagctgtat aacatatatt
     6361 atggttttga gggttgcgat aaggcacggg atattgcctt aagaatcatt aaagcctttg
     6421 aggggaaaat cattcatatc gacaagaagg acaaaccgtt gtatcacatg gctgcatgca
     6481 taatctccaa ttatactgtg accctttccc atgttgccgg caaaatatta gagaacatag
     6541 gaattgatgg ggaggatgcg gcaaaggtct ttttacccct tatacaaaac acagtggata
     6601 acattggaaa gttcggaagt gctggtgcac ttaccggccc tatatcaagg ggagattata
     6661 aggtcgttga agaacacata aaggctctta tcggtttgga accgcaaatt agtgatatat
     6721 ataaaatatt gggccaaaag acaattgacc tggcacttat aaaagggact cttttagatg
     6781 acagtgcaga taagttaagg agtcttttgt cataatattt cgggttgctt gtatggcacc
     6841 ttgtgaattt gattccggta attaaaatat atttgtcaat aagagcataa attaaatata
     6901 gggtgtgggg agaaggttta tggattccga atacttaaga aaactattgg aaaaagttaa
     6961 agatggaaat gtaaccgttg atgaagcttt gctcgaacta aaaaaacttc cctttgagga
     7021 tctgggcttt gttaaggttg accatcacag gaatataaga aacggttatc ctgaggtgat
     7081 ttattgccag ggaaaaaccg ctgaacaggt taaaaaaatt gtggaaaagc tgatggaaag
     7141 caacaataat atcatggcta ccagggccga tgttaaggtc tatgaggcta taaaggagat
     7201 tacttccgat gttgtttacc atgaggcagc aaggattgtg gtagttaaga agagagatat
     7261 acctgtttcc gacaagattg ttgctgtttt gactgccggt acatcggaca ttcctgtggc
     7321 tgaagaagct gcggttacct gcgagaccat gggaaaccgt gttgaaaggg tatacgatgt
     7381 aggagttgcc ggaattcacc gtttgtttgc cagggcggat attattttga acgcaaatgt
     7441 gctgatagtt gtggcgggga tggaaggagc ccttgcaagc gtggtcagcg gtttggtgga
     7501 taaacctgtg atagctgtac ctaccagcgt tggatacggt gcgaattttg gaggtctttc
     7561 tgccctattg tccatgctga acagttgtgc tgcaggaatc ggcgttgtga atattgataa
     7621 cggatttggt gccggttttc tggcggcaat gattaataag ctttaagaag agaggataag
     7681 acatgaagct actttatttt gattgctttt caggtataag tggagatatg gttttaggag
     7741 cccttatcga tttgggtatt gatgttgatg tttttaaaag tgaactgcaa aaactcggtt
     7801 tggacgagtt tgatatttcc gttgaaaaaa agatgaaaaa ttcaataact gtgactgatg
     7861 ttgacgtttt aataaaggat aaggttcatg accatgttca tagccatggc tgcgaaaagg
     7921 aacaaaatca tgaccatggc tgcgaaaagg gacagcatca taatcatggc tgcgaaaacg
     7981 gtcctcatgg cagatatcat catcaccatg cttccgctcg caaccttgca gatattgaaa
     8041 aaatcatcga tgacagcaat ctgaaggata gcgtgaagca gctcagcaag aagattttcg
     8101 gtgagatagc ccgggctgag gcaaaggttc acaataagcc tgtaagtgat gtccattttc
     8161 atgaggtggg agctgtagat tccatagtgg acattgtggg agcttcgatt tgtattgatc
     8221 ttttgaatgt ggaccggatt tattcatcgc ctttgcatga cgggacaggt tttattgagt
     8281 gccagcacgg aaaattgccc gtgccggttc cggcggttat ggagatgctc aaggagagta
     8341 atatacccta catagttgag gacgttaata cggagcttat aaccccaacg ggtattggga
     8401 taataaaaac tattgcatcc ggattcggta caatgccgcc aatgacagta gacaaagtgg
     8461 gatacggagc aggaaaaagg gaaacaggaa ggcttaatgc actaaggtgt gttttgggca
     8521 caacctcgga cgaaggcatt aaggataacg aaatagcggt gctggagacc aatattgatg
     8581 atatgaatcc tgaggtgttg ggctatgtaa tggacttgct ttttgaaagc ggggcattgg
     8641 aagtgtatta tacaccggta tacatgaaaa agaacaggcc cggtgttatg ctgacggttc
     8701 tatcggaaaa agaagatgaa aagaagcttg aggacattat attaaaggag acctcgactt
     8761 tgggtattag aaaaactttt gccacaaggt atgtcatgaa ccgtgaaaac aggtgggttg
     8821 actcagagtt tgggaaaata agcgttaaag aagcatatta tgaaggtatt aaaaagtttg
     8881 cacccgagtt tgaagattgc aggaaggctg cccaaaagca taatgtgccc ttatggaagg
     8941 tttacaatgc ggcgataaaa cagatttaac acaaaaatgc ggaaggctct gtaactttct
     9001 taaaggtgct tcttccgtat ttttttcacc cggataactt acacccatta acggaataga
     9061 tgcggagagg aaaatatgct ttacaacaaa ttttcatcct atttaaaaaa cagatacggg
     9121 acgaaggtat ataagctgcc actgaacctg cctgtcacat gtccgaaccg tgacggtaga
     9181 atcagtgaca agggctgtat tttttgcggt gaggaagggg caggctttga aaaccttccc
     9241 aactgccttg gaataaagca acagctcagc ctgaattcca aatatattgg ggcaaactat
     9301 aattcagaca agtttatcgc atattttcaa aattactcta acacctatct gcccctttct
     9361 gattttaaaa tatatataaa tgaggcgttg tcggaaaaca ttgttgccct ttatatatcc
     9421 acccgacctg actgtataaa ggattcatac cttgagtttt tgaaggaaat caaagcttca
     9481 aaaggagtgg atattgtaat agagttggga ttgcagacgg tcaattacca cactctgagg
     9541 tttttaaacc ggggacacct gttagctgag tttattgacg cagtcttggc ggtaaagtcc
     9601 tatgggctgg aatcctgtgc ccactatata cttgacttgc cgatggattc catggaggat
     9661 ctgattgaag gtgcaagaat actgtcggct ttgggtgtaa gccaagtaaa gtgccattct
     9721 ctttatattt taaagggtac aaccttgggg gacatgtacg aacggggaga aattcggccc
     9781 ttaagtctag aggaatacat agacagggca gtatcttttc tcgaatatct taacccttcc
     9841 attgtggtac aaaggcttat aggccgtgca ccgaaagaaa gaaccctctt ttgcaattgg
     9901 ggaatgagtt ggtggaaaat acatgacctt attgaaaaga aaatgactga ggaaggcagt
     9961 tatcagggca aaagatttga ctacttgaac ggcagggctc ttacatgacg ttgcaatcat
    10021 ttaaatgaga gtaagaggtt ataatgaggt gctactataa gatactgata ttagcttttt
    10081 cataaaacga gggaggataa aaaaataaac gagaaaaaaa taaacgagaa aaaaataaga
    10141 gagaatagag gcgatgaaag gtgaagaaaa atatggaaga aacattgcgg aacttaagtt
    10201 tctggaatat tttcttcacc ttggcataca ctctcagccg attgcatttt tcggccaata
    10261 tttctctttt gcggaatatt actttatcta ggcattcgct ttgggataaa ccttagcata
    10321 cactttcctt aattattctt tgcttctcaa atattcatca atcgcctttg ctgcattttt
    10381 acctgcaccc atcgcaagaa taacagttgc cgctcccgtt accgcatctc caccggcata
    10441 aaccccttct atactggtag aaccggtttt ttcatcggct attatgccac cccatttttg
    10501 agtatccaac ccgggagtgg cagatgttat caatggattt ggagtttggc ctatggcaat
    10561 gataactgtt tctacttcga taacatgttc cgagcctgcc tttgcaaccg gtcttcttct
    10621 tccggataca tccggctcac ctaactccat ttcaacacat tccataccct ttacccagcc
    10681 gtcctctgtg ccgagaatcc ttgtgggatt tgtaaggagt ttgaatatga tgccttcttc
    10741 ttttgcgtgg tgtacttctt caagtcttgc aggcatttcg gcttcagaac ggcggtatac
    10801 aatatagacg ttttgtgctc caagcctttt agcacatctt gccgcatcca ttgcaacgtt
    10861 tccgccgcca acgacagcaa cgtttttgcc gatctttaca ggtgtatcat aatccgggaa
    10921 cttatatgcc ttcataaggt ttattcttgt gagaaactca tttgaagagt acacaccatt
    10981 gaggttttca cccgggattt tcataaagct gggcaatccg gcacccgaac ctataaatat
    11041 ggcttcatag ccttcttctt taagttcttc gagagtgagc acctttccaa taaccatatt
    11101 ggttttaatt tccacgccaa gcttcttaat agtatctatt tctttctgta ccagttcttt
    11161 tggcagcctg aactcgggaa ttccgtacat caatacgcct ccgggcacat ggaaagcttc
    11221 aaagacagtg acatcataac ccatttttgc cagatcaccg gcacatgcca gccctgcagg
    11281 accagaaccg attattgcaa cttttttccc actcttattg ggcttttcaa caaaatcttc
    11341 cgcatttttt atatgccagt ctgctgcaaa cctttcgagc cttcctatag cgaccggttc
    11401 accctttata ccccgcacgc agcgttgttc gcactgggtt tcctggggac acacccttcc
    11461 gcatattgca ggaagactgt tggtttcctt aattttatta tatgcctctt caaatttacc
    11521 ctgagcaacc agactaataa actcaggaat ttgtacattg accggacacc cttgcacaca
    11581 aggcttgttt ttgcagttta aacacctttg ggcttcttcg attgccatct gctcggtgta
    11641 acccgatgca acctctaaga agtttttatt cctgacatcc ggtgcctgtt cgggcatggg
    11701 aacctttttc aatgacatgt taggcatctt tacctatccc ccaatctaca ggaatgttct
    11761 tcgcttttat aaatattctg tcttctaatt gcttcatcaa aatccaccaa gtgtccgtca
    11821 aaatcgggac cgtccacaca tgcgaatttt gtttctccgc ccactgtcac cctgcaccct
    11881 ccgcacatac ctgtaccgtc aaccataaca gggttcatgc taacaatggt ctttattccg
    11941 tactgctttg tgaggctgct tacaaacttc atcattacca aaggaccaat tgctattacc
    12001 aaatcgtatt tgttgccttc ttctatcaat tccttaagga catcggtaac gaagcctttt
    12061 ttgccgttgg agccatcgtc cgtagtaatg taaagcttgc tgctaacggc tttcatttta
    12121 tcctccaata taataaggtc tttatttcta aaacctataa ttgaatggac ctcgacaccc
    12181 atcgagttta attttttagc ctgaggataa gctatcgctg ttcctaggcc tcctccaatt
    12241 actgctgctt ttttgatgcc ttcaaaatgt gatgcttttc ctaaaggacc gacaaaatca
    12301 agtatgtggt ctccttcatt aagttctccc aaagcaatgg tggtttttcc cacctcctga
    12361 aatataatgg taacggtgcc ttgggttcga tcgtaatccg caatggtcaa aggaattctt
    12421 tctccgtttt cacttatcct gagaatgata aactgtcccg gttctgcctt tttggctata
    12481 tgtggagcat ctatctccat caacttaacc tgagggttaa gaacctcctt cttaactatt
    12541 ctaaacatta ttgggactcc tttcaaataa aaatcagatc actcatcacc actaatttta
    12601 aatataatat gtccttaagt caatcatggt gcaagaaaaa ataatttcat ataataaaca
    12661 cgtcaatata taaatacgtt atagttttat aaatacgcgg atattttagt tttagtatat
    12721 gaaactatcg aataataaac ttgcatatat tttgttaaga gaatcaatca aaaacgcagt
    12781 taaaaccatt ggtatagcgg ctaaacacaa taatgacagg attacattaa atgggtaaaa
    12841 ttttgaaatt cgaaattttt taaattaata cttgcaattt tgttgctctt ctgttacaat
    12901 tcaaatgcaa ggtatttagc attcaaaaaa taacgctttt ggtgtatata tagttactga
    12961 taaatttcaa aagattatca gtgataccat cataatacac caacaagatt gtatatttgt
    13021 ggatgatgaa tgtcgacagt tttaaatatg gagggagtta aaatgaagct attatccaat
    13081 attatggaac aagttattaa aaagaaccca aatgagcagg aatttcatca agcggtggaa
    13141 gaagtattgg aatcattaga ggttgttgct gaaaacaacc cggaatactt aaaggccggg
    13201 atatttgaga ggatagttga gcctgaaaga cagataattt tcagagtacc ctgggttgat
    13261 gacaacggaa aagtacaggt aaacagggga ttcagagttc aattcaacag tgccatagga
    13321 ccttataagg gaggtctcag attccatccg tcggtttgcc tcggaattat taagttcctt
    13381 ggatttgagc agattttcaa gaattcactt acaggtcttc ctatgggggg aggaaaaggt
    13441 ggaagtgatt ttgatcccaa gggtaagtcc aacggtgaaa taatgagatt ttgccagagc
    13501 tttatgacgg agctttgcaa acatatagga ccggataccg atgttcccgc gggagatatc
    13561 ggtgtaggcg gccgcgaaat tggttatatg tatggaatgt atagaaaaat cagaaatgag
    13621 tttacaggta ctttaacggg taaaggtttg acatggggcg gaagtcttgc aagaaccgaa
    13681 gctacgggat atggattgtg ttatttcatg gaagaagcta tgaagacaat aaaaggaaaa
    13741 tcctttaaag gcacaacagt agtaatatcc ggttcgggta atgttgccat ttatgctact
    13801 gagaaggcac agcagttagg agcaaaagtt gtggcattaa gcgattccaa cggatatatt
    13861 tacgatcctg atggaattaa acttgatacc gttaaacaaa taaaagaagt agagagaaaa
    13921 agaatcaaag aatatgtcaa gcatcatcct aatgcccagt ataccgaagg tt
//
LOCUS       DAOOJC010000007        13959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_4157_length_13959_cov_3.929445, whole genome shotgun sequence.
ACCESSION   DAOOJC010000007 DAOOJC010000000
VERSION     DAOOJC010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 13959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13959
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4157_length_13959_cov_3.929445"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLA01_00435"
     CDS             complement(<1..439)
                     /locus_tag="PLA01_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPC99799.1"
                     /translation="MGVYETRDIIYGFITYDDWEREIINHPVYQRLRRIKQLSLTDMV
                     YPGANHTRFEHSLGVMHLATLFYDNIIKKRQRFLKEVLNFNDAGLERDRRIIRFAALL
                     HDIGHSPFSHAGEELMPFIPQGNPKYEEGKDKRFSHEDYSIAII"
     gene            complement(641..1165)
                     /gene="leuD"
                     /locus_tag="PLA01_00440"
     CDS             complement(641..1165)
                     /gene="leuD"
                     /locus_tag="PLA01_00440"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPC99800.1"
                     /translation="MKTFSGKVLFLDRSDINTDEIIPAKYLTEITKEALKPYLLEDLR
                     LDGFDPKKDVEGKSVIVTRANFGCGSSREHAPWALEVNNINVVIAQSFARIFRQNMYN
                     CGMFAIELPKEKIDYLFDNYENKETSIEVDVDRDKIIVSSAGKSEEMDFAVGEFDKAL
                     VKEGGWVGYADKKY"
     gene            complement(1167..2462)
                     /locus_tag="PLA01_00445"
     CDS             complement(1167..2462)
                     /locus_tag="PLA01_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPC99801.1"
                     /translation="MNKTIAEKIFDAHRVDMPFPDTHVLKLDAVFCHEITTPIAITDL
                     QNRGMDRVFDAEKIKAVIDHVTPAKDSKTAEQGKILRDWARRHGIKDFFDIGRNGVCH
                     ALFPEKGFVRPGYTIIMGDSHTCTHGAFGAFAAGVGTTDLEVGILKGVCAFRSPKTIK
                     IVLNGKLKPGVYAKDVILFVIKELTVNGATNRVIEFTGPIVDEMSMESRMTLCNMAIE
                     AGGTCGICYPDAKTVDYLWDYIKDEFSTKEEAIKRYSEWKSDEGAEYEKVYEYDLSDL
                     EPMVTFGYKPDEVKTVSEMTGTKVDQVYIGSCTNGRIEDLRIAAEVLKGKKISDNVRG
                     IVSPATPDVYKAALKEGIIEIFQEANFCVTNPTCGACLGMSNGVLAAGEVCASTTNRN
                     FNGRMGKGGMIHLMSPATAAATAIKGYIINSGLYNEKGE"
     gene            complement(2914..3005)
                     /locus_tag="PLA01_00450"
     tRNA            complement(2914..3005)
                     /locus_tag="PLA01_00450"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:gga)
     gene            complement(3252..3947)
                     /locus_tag="PLA01_00455"
     CDS             complement(3252..3947)
                     /locus_tag="PLA01_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HPC99802.1"
                     /translation="MKKLVIYLLLLSFVLVGCSGEKKEKLQASELTDQYQLVTQTEEL
                     SEGQSTMSLSYPVVKDLIDKRIEDKINETIKNRVEEYKGLCGLGEDMGIQETSTVRYE
                     VPYKSKEKLSIKFYIESQMEDEMSDIIIDTYTFDLNSAEDLFLSELFKNKKYKKKINS
                     ILKEKFDSLEVETIKEFVSIEETQGYYLKGNSLVIYFQTLDYTMDEPLEFEIPLDDLK
                     DILKKPIPEGEIL"
     gene            complement(4121..4978)
                     /locus_tag="PLA01_00460"
     CDS             complement(4121..4978)
                     /locus_tag="PLA01_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HPC99803.1"
                     /translation="MKNKFKDDLIIMLKGYTIGSSMSVPGVSGGTMAILLGIYDKLIS
                     SVSNFWKDIKGNTLFLLKFCIGAGAGICSLAFLLKWMLEKFPLPVSVFFLGAVVGGIP
                     ALYKKTKESSLRISSGIYFLLGFLVVISIGFIPEGSVDVSIGSGVTHYLMLLLVGIII
                     ALALVLPGISTSHMLLVLGMYDAILGAVTSFDVVYVGILGIATLIGVFLITKPIEWTL
                     NKFPHQTYCAIIGFVLGSTAEIFRDKILPAIPESPDAFWWISSVLISIVTFVLGFLAI
                     KSLSKFSND"
     gene            complement(5157..5636)
                     /gene="rlmH"
                     /locus_tag="PLA01_00465"
     CDS             complement(5157..5636)
                     /gene="rlmH"
                     /locus_tag="PLA01_00465"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPC99804.1"
                     /translation="MKLTVIAVGKLKEKYLKEGINEYSKRLSRFCELEIIEVNDEQAP
                     DSLSSVQEEQVKKKEAERVIKRLKPGTVLIIMDVHGEKLGSPDFANRINSFFLSGKSH
                     ITFVIGGSLGIDKELLGMADLRLSLSDMTFPHQLTRLILLEQVFRAFKIINGETYHK"
     gene            complement(5698..6489)
                     /locus_tag="PLA01_00470"
     CDS             complement(5698..6489)
                     /locus_tag="PLA01_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPC99805.1"
                     /translation="MIKFCSLYSGSSGNAIFLSDGNTKILIDTGLSGKRIFEALVSIG
                     EKPSELSAILVSHEHSDHICGVGVVSRKVDVPIYANQNTWEAMEHSIGPVSLKNKMYF
                     DTGREFEIGNITIKAFPIPHDAAEPVGFNFFIGNSKVTTATDIGHMNKDLLCHIEGSD
                     LLLIESNHDVEMLKVGPYPWPLKKRILGELGHLSNEMAGKVVAYLAEKGTRNFLLGHL
                     SKENNFPELAYETVRSILREKNIDIEKDIKLSVALRDRAGRVVEM"
     gene            complement(6540..7793)
                     /locus_tag="PLA01_00475"
     CDS             complement(6540..7793)
                     /locus_tag="PLA01_00475"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPC99806.1"
                     /translation="MEKLIITGGKPLRGEVSISGAKNSAVAILPASILVDGPCRIENV
                     PDISDVKALADILKELGADITFEDNEVLNIKTQGLKSNIATYDMVKRLRASYYLMGAL
                     LGKFKKAEVSMPGGCDFGFRPIDQHIKGFEALGAKVEIDRGIVKLEAEKLKGCQIYLD
                     VVSVGATINIMLAAVKAEGITSIENASKEPHVVDVANFLNAMGANIKGAGTDVIKIKG
                     VSSLAGGATHSVIPDMIEAGTFMIAAAATKGDVTIRNIIPKHVESLSAKLVEMNVRID
                     EGGDYMRVVGTDRVDKVNIKTLPYPGFPTDLHPPVAVLLCRGNGTSTITEGIWDNRFQ
                     YVDELKRMGAKIKVEGRMAVIEGVPRLSGAPVRATDLRAGAAMVIAGLVAEGVTEVYN
                     VEYIDRGYENLETKLKGLGADISRK"
     gene            complement(7894..8730)
                     /gene="yycI"
                     /locus_tag="PLA01_00480"
     CDS             complement(7894..8730)
                     /gene="yycI"
                     /locus_tag="PLA01_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HPC99807.1"
                     /translation="MDWSRAKNILILIFILLNVFLSAVIINELRGSRVTKETISNTRL
                     ALKERGVEINCSIPDYNGSAGNLVYKKISIDRKKLIQNIFGAGTDIDNLLMQEVVERD
                     GKELRFKDEYSFSYKNTEPEGMISGLNNPDKILGYVEKILKGTEIPVLDYKIDSVEQD
                     KKGTTTYVFRQKYKNFWIIENHITVSLSDKDLEVECRYQKVDGIRNSKKVMPVHQVLF
                     KNHETFKDITINRIDLGFKNPKRDDGRRESDGIPVWCIEIQDKDNTVRRYFNAYDGDE
                     IE"
     gene            complement(8742..10193)
                     /locus_tag="PLA01_00485"
     CDS             complement(8742..10193)
                     /locus_tag="PLA01_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99808.1"
                     /translation="MKGLNRERFKLYILFLLVITSFIQVGILWYYQNQRVPTNFLWGF
                     FNSEKNETVYDIGYFFKPYRIVVTEGFENPHWVIDEDHEFYSMLWDEARVYISNLLTK
                     KGVNHQGEYNREYWGEVVLRKSFVIEFKTNISTELLAAFLNVNPDSDFKPGGIYKMAI
                     LPSENINRNLTIYVYNGTSVNKYVMPFEQKGLHINDYDNIIGQLYEEDSDGYNVVKEY
                     ISNYVYDYLSPDVLIVAKGNSFREFENLVCHVPDRIKNTRSNEELADMAQVILGEDND
                     EYMKNIDVYNVIVFQNLSNMYRIYRDGLLEYRYLYVIDNNDKGSESEALNKALNFIKS
                     REELISPEIDIYLSGITRNQNYYTFTFDYKYKGRPVSLDDYKINNKDGKSLNHAITID
                     VNSKRVLNCYWLLKDFKKGNEKFNLNVKFEYMFEDVFANNSGLDRNDFSIRDVNVVYE
                     IKYSADVQPLKPVWSIETMQGKKYIVPMREKPE"
     gene            complement(10209..10739)
                     /locus_tag="PLA01_00490"
     CDS             complement(10209..10739)
                     /locus_tag="PLA01_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99809.1"
                     /translation="MMKKMIVMFSVFIFAISLCINAFAQDDIPEVFSNKDTQETAQFL
                     VTITRPEGDETTFKESYVLCGNSDKENITIKLLMYNESTEKFEPFKNIDGEDTWQIGV
                     SGFFMKEITLPKNGANKIRIIAYRDSDGKANMLKPGENLQVNTFTVTLLEKSVKDSIK
                     NGFLRITDILNVLFGK"
     gene            complement(10850..12649)
                     /locus_tag="PLA01_00495"
     CDS             complement(10850..12649)
                     /locus_tag="PLA01_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC99810.1"
                     /translation="MIFGRLNLLRSLQWRLVTIFILLTFVLMISAGISLNYFVETFYY
                     DTFKAGIESGFEYWGIDNNASPEKEEILEYLTANNKANAMSLFSINNFRSFTVADMKT
                     NEILYTDERMPYRETLAHDIQNSRNYLAVLAGKAGDRGKLIRINEKAFFDYARRIGNS
                     DYVLYFRYDREEWAGAMEAFNNIIKLSMVIAVILSLIFGYALSKTITVPIVNLMHKAR
                     KMAAGDFDQMVEVKSEDEIGKLTKTFNFMARELKNTLNQISREKSKIETILNFMTDGV
                     IAFDINGEVIHINPAAKRILEIDDFSFDFNEFSKKYELGVSIEEIQYLEKYSTKEVVI
                     DIGEKFVRMYFALFTDKENNAEGIIAVLHDITEEQKLENMRKEFVANVSHELRTPLTS
                     IKSYSETLLDGALEDREAAEKFLGVINSEADRMTRLVKDLLQLSRLDNRQMKWNMQEF
                     SFEDLVKSCVEKVRFSSKEKNQTLECFTIGEITNIVGDKDRIEQVVLNILTNAIKYTP
                     VEGKITIYIGKMYGDVYVKVADSGIGIPKEDTSRIFERFYRTDKARSREMGGTGLGLA
                     IAKEIVEAHNGKISVSSELGKGTEVTVRLPICN"
     gene            complement(12696..13391)
                     /gene="yycF"
                     /locus_tag="PLA01_00500"
     CDS             complement(12696..13391)
                     /gene="yycF"
                     /locus_tag="PLA01_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HPC99811.1"
                     /translation="MSAKILIVDDEKNIVDILKFNLTKEGFSSIEAYDGEQAIELAHS
                     EKPDLILLDIMLPKMDGFTVCRKLRETISTPIIMITAKEEEVDKVLGLELGADDYITK
                     PFSTRELMARVKANLRRVDSSDLQKKPNNYLKFGDLEIDIEHYEVKRDNNVIELTLRE
                     FELVKFLALQQGQIFSRESLLEKVWGYEYYGDVRTVDVTVRRVREKIEKDPSNPSYIM
                     TKRGVGYYFNKVS"
     gene            13685..13903
                     /locus_tag="PLA01_00505"
     CDS             13685..13903
                     /locus_tag="PLA01_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPC99812.1"
                     /translation="MSKDLLMVVITKMHIRLNELIEKNDYDLLCEEVQLYSRRLDKFL
                     SYYYKADEKTPSFEYDFRVLDNTVKCSV"
BASE COUNT         3997 a         3291 c         2063 g         4608 t
ORIGIN      
        1 taattattgc aatactatag tcttcatgtg aaaacctttt atctttccct tcctcgtact
       61 taggattacc ttgaggaata aaaggcataa gctcttctcc agcgtgcgaa aagggtgaat
      121 gccctatatc atgtagtaga gccgcaaacc tgataatcct tctatccctc tcaagccctg
      181 catcattaaa attcaatact tctttcaaaa accgctgccg tttttttata atattatcat
      241 aaaaaagtgt agcaaggtgc ataaccccca aagaatgctc aaacctggtg tgattggccc
      301 caggatatac catatcggtt aacgaaagtt gttttattct cctcaaacgc tgatatacag
      361 gatgattaat tatttcccgt tcccaatcgt catatgtaat aaaaccatat attatatctc
      421 ttgtttcata tacacccatt acaataaacc tcactactta catatgatgc aaaaacatag
      481 ttgaagtact taaaagtaca gtgtttgtcc tcattttatt ttcattactt aacaactgct
      541 acatatatta taccgaacat atgttcattt gtaaatagtc aatatattta tcaattttat
      601 cacaaaacca tttttcgctg ttatccgttc ctgccctgtg tcaatacttt ttgtcggcgt
      661 aaccaaccca gccgccttct tttacaaggg ctttgtcaaa ctctcccaca gcaaaatcca
      721 tctcttcaga cttaccagcc gatgaaacaa taatcttgtc tctatcaaca tcaacttcta
      781 ttgatgtttc tttattttca taattatcaa aaaggtaatc gatcttttct ttcggcagct
      841 caattgcaaa catgccgcag ttgtacatgt tctgcctgaa gatccttgca aagctttgtg
      901 caattacaac attaatgttg ttaacttcca aagcccacgg ggcatgttcc ctagatgaac
      961 cgcacccgaa attcgccctg gtgacgataa cggatttgcc ttcaacatct tttttgggat
     1021 caaagccgtc cagcctcaag tcctccaaaa ggtaaggctt tagtgcctcc tttgttatct
     1081 ccgtaaggta ttttgcagga attatttcat cggtgtttat gtcgcttcta tctaaaaaca
     1141 gtacttttcc gctaaaagtc ttcatcttat tccccctttt cattatataa accggagttt
     1201 ataatatatc ctttaattgc cgtagctgca gcagttgcag ggctcatcaa atgaatcatt
     1261 ccgccttttc ccatacgtcc gttaaaattc cggtttgttg tagaggcaca tacctcacct
     1321 gcggccaata ctccgttgct cataccaagg caggccccac aggtaggatt tgtcacacag
     1381 aagttggctt cctggaaaat ctctataatt ccttctttaa gtgccgcttt atacacatcc
     1441 ggtgtggcag ggcttactat tccgcggaca ttatcactga tctttttgcc ttttaatacc
     1501 tcggctgcaa tcctgaggtc ttcaatccgg ccgttcgtgc agctgccaat gtagacctgg
     1561 tcaacttttg tccccgtcat ttcgcttacg gtcttaacct catccggctt atatccgaaa
     1621 gtaaccatcg gttccaaatc tgacaggtca tactcatata ctttctcgta ttcggcacct
     1681 tcatcggatt tccactcgga gtacctttta atagcctctt ctttagtact aaattcatct
     1741 ttaatataat cccacagata atccactgtt ttggcatcgg ggtaacaaat accgcaggtt
     1801 ccgcccgctt caattgccat attgcacaag gtcattctcg actccatgga catctcatcc
     1861 acaataggac ccgtaaactc gataaccctg ttggtagcac cgtttacggt taattctttt
     1921 attacgaaaa gaattacatc cttggcatat acaccgggtt ttaatttacc gtttaatact
     1981 atctttatgg ttttaggaga acgaaaagca catactcctt ttagtattcc cacttccaaa
     2041 tccgtagtcc caacccctgc agcgaaagct ccaaaagctc catgggtgca ggtgtgggaa
     2101 tctcccatta ttatggtgta tccgggcctt acaaatcctt tttcggggaa taaggcatgg
     2161 catactccgt tgcgtccgat atcaaagaaa tctttaatcc cgtgccttct ggcccaatca
     2221 cggagtattt ttccctgctc ggctgtttta ctgtcctttg ctggtgtaac atggtcaatt
     2281 actgctttga ttttttcggc atcaaaaacc ctgtccattc ccctgttttg caaatcggtt
     2341 attgctatag gtgtcgtaat ttcatgacag aataccgcat caagctttaa cacatgagtg
     2401 tcgggaaagg gcatatcaac cctgtgggca tcaaatattt tttcagctat tgttttattc
     2461 atacaaatca ccctctatta tttattccgt agttcataat ggttcgttaa ttgatagaag
     2521 tattaatgtc ttttaaaaat atgttatcat ccacaaataa atatattata ttaactatgt
     2581 tatcattata atatttcgta tataatatta cttcctaaaa tatatctaaa atatattgaa
     2641 tttttaatga ttattgacct tttagaaaag cttaacatcg aatgatgcgg ataatagtaa
     2701 catccttcag tcatcattgt gcttgtgaat tatgtaatat caaaaaaact gcttatttct
     2761 gccatgggca aaagtagaat tcgtctttat taacattgat tagtttatgg cacaaatttg
     2821 tcacaatttg atgggtaata aaattaatta tggaatatat ctgctatctg caaaaaacta
     2881 aacccctctc aaacctagta tttgcaaggg gtttggcgga gagagaggga ttcgaaccct
     2941 cggtacgcta ttaacgtaca cacgatttcc agtcgtgcgc cttagaccag ctcagccatc
     3001 tctccattta agtatcacat aaataaagcc tttcggctat tatatgatgc actattcagt
     3061 tcaatcaccc gaatattata acacggtaaa ctgtaaaaaa gcaatagaaa atacaaatta
     3121 aaatattatt ttctatgcag taatatatac catggagtta aaaatcaagg aagcttccat
     3181 tcaataaacc ctaagcccct atgggcttag ggtttattga aatcttttcc attatccgaa
     3241 tttattatac cctacagtat ttcgccttcc ggtatcggct ttttaagaat atctttcaaa
     3301 tcgtccaaag gtatttcaaa ctcgagaggt tcatccattg tgtaatccaa tgtctggaag
     3361 tatattacaa gactatttcc cttaaggtaa tatccctgtg tttcttcaat acttacaaat
     3421 tctttgatag tctcaacttc caagctgtca aacttttctt tcagtattga gtttatcttc
     3481 tttttatatt ttttgttctt aaataattca gaaagaaaca aatcttccgc actgttaaga
     3541 tcaaaggtat atgtatcgat aataatgtcg ctcatttcat cttccatctg ggattcaata
     3601 tagaacttaa tgctaagttt ttcttttgat ttgtatggaa cttcatatct tactgttgaa
     3661 gtctcctgaa ttcccatatc ttcaccgagg ccgcacagtc ctttatactc ttcgactctg
     3721 ttttttatgg tttcattgat tttgtcttca atcctcttgt caattaagtc ctttaccact
     3781 ggataactta aactcattgt tgattgtccc tctgaaagct cctcagtttg tgtcactaat
     3841 tgatattggt cggttaactc gctggcctgc agcttctcct tcttttcacc agaacacccc
     3901 actaaaacaa aggataataa gagcaaataa attactaact tcttcactgg aatatccctc
     3961 cacttgttat aaaatattaa tgtctatttt ttaaagcatg tcctctatta atataacata
     4021 tctttaagaa aaattaaata taaaaaaaat cccgctaaaa aagcagcaac ggaattcttt
     4081 tccgaagctg ccccttatta tcatcttttc ttacgtctaa tcaatcattt gaaaatttgg
     4141 acaaggattt aatggctaaa aatcctaaaa caaatgtaac tatagaaatc agaacagaac
     4201 ttatccacca aaacgcatcg ggactttcag gaattgcggg taatattttg tcacgaaaaa
     4261 tctctgctgt ggagccaaga acaaatccaa taatcgcaca ataggtctga tggggaaatt
     4321 tatttaatgt ccattcaatt ggttttgtaa tcagaaaaac acctattaac gttgcaattc
     4381 ccaatattcc aacgtaaaca acatcaaagc tcgttactgc accaagaatg gcatcataca
     4441 tacccaatac cagcagcata tgggaggtac taatgcccgg tagcaccaaa gccaaggcaa
     4501 ttataatacc aacgagcagt aacatcaaat aatgagtgac tcctgaaccg atgctgacat
     4561 cgacacttcc ttccggaata aatccaatgg atattacaac gaggaaaccc aacaagaaat
     4621 aaatacctga agatattcta agcgaagatt cctttgtctt cttatacagt gcaggaatac
     4681 caccgactac cgcccccagg aaaaacacgg atacaggcag cggaaatttt tccagcatcc
     4741 attttaataa aaatgcaagg gaacaaatac cggcaccggc tccaatacag aacttcagta
     4801 aaaataacgt attgcccttg atatccttcc aaaaattact gactgagctt ataagcttat
     4861 cataaattcc caacagaatg gccatagtac cgccgctcac tccgggaacg ctcattgacg
     4921 agcctatagt atatcctttt aacattatta ttaagtcatc tttaaacttg ttcttcatct
     4981 acataaatac acctttaatt gcggtgtatt cctcctttta atctatgatt tttcatgaaa
     5041 ataaccgcac tgaaattata gcaccatata ttactagtgt caagcacaat cctttcgttg
     5101 cttatacatc actttatacc tcatcatcga ggtatacata ataatactgc tttccatcac
     5161 ttgtgatatg tctcgccgtt tattattttg aaagccctga atacttgctc taaaagtatc
     5221 aatcttgtca gctggtgtgg aaatgtcata tctgaaagtg aaagccttaa gtctgccatc
     5281 cccagaagct ctttgtcgat tcccagcgac cctccaataa caaatgtaat gtgtgattta
     5341 cccgaaagga aaaaagaatt gatcctgttg gcaaaatccg gagaacccag tttttcgccg
     5401 tgcacatcca taatgattaa aacagtcccc ggcttgagtc tttttatgac cctctcggcc
     5461 tcctttttct taacctgttc ctcctgaaca ctgctcaagc tgtctggtgc ctgctcgtca
     5521 ttaacctcaa ttatttccag ctcacaaaac ctggaaagcc ttttactgta ttcatttatg
     5581 ccttccttaa gatatttttc ttttaatttt cctactgcta ttaccgttaa cttcatatct
     5641 cacaccaata aaatactgcc ggacacaact ttcccggctt ttataaaatt tcttttatca
     5701 catctctacg acccggcctg cccggtccct caacgcaacc gacagcttta tatctttttc
     5761 aatatctatg tttttttctc tcaaaatgct gcgtacagtc tcatacgcca gctcaggaaa
     5821 attattttcc ttactcaaat gccccaataa gaagttcctg gtccccttct ccgccaaata
     5881 cgccacaacc ttccctgcca tttcattgga caggtgcccc aactctccca gaatcctttt
     5941 ctttaatggc catgggtacg ggcctacctt caacatttcc acatcgtggt ttgattcaat
     6001 cagcagcaaa tcgcttcctt cgatatggca taacaaatct ttgttcatat gtccgatgtc
     6061 ggtagcggtt gtaaccttac tgtttccgat aaagaagtta aatcccaccg gttcggcggc
     6121 atcgtgaggt atcgggaaag cctttatggt tatgtttcct atctcaaact ctcttccggt
     6181 atcaaaatac attttatttt tcaggctcac agggccaatg ctatgctcca tagcttccca
     6241 ggtgttctgg tttgcgtata tcggaacatc caccttcctt gatactacgc ccaccccaca
     6301 aatatggtcg ctgtgttcat gggacactaa aatagcactt aattccgaag gcttctcacc
     6361 aattgaaacc agtgcttcaa aaatcctttt accgctaagg ccggtatcaa tcaggatttt
     6421 tgtatttcca tccgaaagaa atatggcatt gccactgctt ccgctatata aactgcaaaa
     6481 ctttatcatt cgtaatctcc ttatagaaca gagaaccgtc ccctgttcta tgttctattc
     6541 tattttcttg atatatcggc acccaggccc ttcagcttgg tttccaggtt ctcatatccc
     6601 ctgtctatgt actctacatt atacacctct gtcacacctt ctgcaacaag tccggctata
     6661 accattgccg cccctgctct gaggtccgtc gccctcaccg gtgcccctga cagcctcgga
     6721 actccttcaa ttaccgccat tcttccttca acctttatct tagcccccat ccttttaagc
     6781 tcgtcaacat actgaaacct gttatcccat atcccttcag ttattgtgct ggttccgttt
     6841 cccctgcaaa gcaaaacggc aaccggggga tgaaggtctg ttggaaatcc cgggtatggc
     6901 agcgtcttaa tattcacctt atccaccctg tcggtaccga caaccctcat ataatctccg
     6961 ccttcatcaa tcctcacatt catctcaaca agcttcgcac ttaaagactc cacatgcttg
     7021 ggaattatgt tcctaatagt tacatctccc tttgtggcag ctgcagctat cataaacgtg
     7081 cctgcttcta tcatgtccgg aataaccgaa tgggtcgccc ctcctgccag actggataca
     7141 ccctttattt tgataacatc cgttccggcc cctttaatat tggcacccat ggcatttaaa
     7201 aagttcgcaa catcaactac atgcggctcc tttgaagcat tctcgataga ggttattccc
     7261 tccgccttaa cagccgctag cattatattt atggttgcac caacacttac cacgtccaaa
     7321 tatatctgac agcctttcag cttttcagcc tccagcttaa caattccacg gtcaatttca
     7381 actttcgcac ccaatgcctc aaaccctttt atatgctgat cgataggcct aaagccaaaa
     7441 tcacatcctc cgggcattga tacctctgct tttttaaatt tccccaaaag agctcccatt
     7501 aaataatagg acgccctaag tctttttacc atatcgtatg tggcaatatt cgatttcaag
     7561 ccttgagttt ttatattaag aacctcatta tcctcaaaag taatatcggc acccaactct
     7621 ttcaatatat cggcaagtgc cttaacatca ctgatatcag gtacgttctc tattctgcac
     7681 ggtccatcta ccaaaatgga agctggtaaa atcgcaacag cagaattttt tgcaccgctt
     7741 atactaactt cgcctcttag aggttttccc cctgttataa ttaatttctc caattaatcc
     7801 cccacccgcc aattattact ttagtatgag caaatatctt cttcttagat tatactacta
     7861 aattagaaca ttttacacat tattttttaa atcctattca atttcatccc cgtcatacgc
     7921 attaaagtat cttctgaccg tattatcctt atcctgaatt tcaatgcacc atacgggaat
     7981 accgtccgat tcccttctgc catcatccct tttcgggttt ttaaagccca gatcaatcct
     8041 gttaattgtt atatccttaa aggtttcgtg attcttaaac agcacctgat gtaccggcat
     8101 taccttttta ctgtttctta tgccgtcaac cttctgatat ctgcactcaa cttccaaatc
     8161 cttatccgat aaactcacgg taatgtggtt ttcaataatc cagaaattct tgtatttttg
     8221 cctgaaaaca taagttgttg ttcctttttt gtcttgttcc acagaatcaa ttttataatc
     8281 caatactggt atttctgttc ccttaagaat tttttcaaca taacccagga ttttatccgg
     8341 attatttaag cctgatatca tcccttccgg ctccgtgttc ttatatgaaa agctatactc
     8401 gtctttaaat ctcagttcct ttccgtccct ttccacaacc tcttgcatca gtaaattgtc
     8461 aatatcggtt ccggcaccaa atatattttg aatcagcttt ttgcggtcga tacttatttt
     8521 tttgtaaacc aggttacctg cactcccgtt ataatccggt atgctgcaat taatttcaac
     8581 tcctctttcc ttaagagcca gtcttgtgtt tgaaatagtc tcctttgtaa ctctgcttcc
     8641 ccttaattca tttattataa cggcactaag gaaaacattc agaaggataa aaatcaatat
     8701 aagaatattc tttgctcttg accaatccat cgctatcctc cctattccgg cttttccctc
     8761 atgggaacaa tgtatttttt accttgcata gtctcaatac tccaaacagg cttcaacggc
     8821 tgaacatcgg cagaatactt tatctcatat acaacattta catccctgat ggaaaaatca
     8881 ttcctatcca atccactatt attggcaaat acatcctcaa acatatactc aaatttaaca
     8941 ttaagattaa atttttcatt tcccttttta aagtccttta aaagccaata gcagtttaac
     9001 accctcttac tgtttacatc tatagttatg gcatgattta agctttttcc gtctttgttg
     9061 tttattttat aatcgtcaag ggaaacaggt ctgcccttgt atttatagtc aaaggtaaag
     9121 gtataataat tttgattcct cgttattccc gacagataaa tgtcaatctc cggggaaatt
     9181 aattcttccc tgctcttgat aaaattcagt gccttgttca aggcctcact ctcgctgcct
     9241 ttatcgttat tgtcaattac ataaagatat ctgtattcca acaaaccatc cctgtaaatc
     9301 ctatacatgt tgctgagatt ttgaaatact attacattat aaacatcaat atttttcata
     9361 tactcatcat tgtcttctcc aagaataact tgtgccatat ctgccaattc ttcattggaa
     9421 cgggtatttt tgattctatc cggaacatgg caaacaagat tttcgaattc ccggaaacta
     9481 ttgccctttg ccactatcag tacgtcggga ctcaaataat catacacata gtttgatatg
     9541 tactccttaa caacattata tccatcgctg tcttcttcgt acaattgacc tatgatatta
     9601 tcataatcat tgatatgtaa tcctttttgt tcaaagggca tgacgtattt gttaacactc
     9661 gtaccattgt acacatatat cgttagattt cgattgatgt tttccgatgg aagaattgcc
     9721 atcttgtaaa ttccgccggg tttaaaatca gaatccggat tgacattcag aaatgctgca
     9781 agaagttccg tacttatgtt ggtcttaaac tcaattacaa aggatttcct taatactacc
     9841 tctccccaat actccctgtt gtattcgccc tggtgattaa cgcctttttt agtcaaaagg
     9901 ttactgatat aaactctcgc ttcgtcccac aacatagaat aaaactcatg gtcctcatca
     9961 attacccaat gcggattctc aaacccttct gtaactacaa tcctgtaggg tttaaaaaaa
    10021 tatccgatat catataccgt ttcatttttt tctgaattaa aaaaacccca taaaaaatta
    10081 gtaggcactc tctgattttg ataataccaa aggataccta cctgtataaa actcgttata
    10141 actaataaaa acaatatgta caacttaaat ctttcccgat ttaatccctt cattcatata
    10201 ccagaccttt attttccgaa tagaacgttc aaaatatctg taatccttaa aaatccattc
    10261 ttaattgaat cctttacgct cttttcaagc aatgtaacag tgaaggtatt tacctgcaaa
    10321 ttctctccgg gtttaagcat gttcgccttt ccatcagaat ctctataagc aattatccta
    10381 atcttgttgg caccattttt ggggagtgta atctctttca tgaaaaaccc cgaaactcct
    10441 atttgccatg tatcctcacc gtctatattc ttaaagggtt caaacttttc tgtggattca
    10501 ttgtacatga gaagttttat tgttatgttt tccttatcgg aattgccgca gagcacgtat
    10561 gattctttaa acgtagtttc gtcaccttcg ggacgggtta tagtaaccag aaactgtgcg
    10621 gtttcctgag tatctttatt cgaaaacacc tcaggaatat catcctgtgc aaaggcatta
    10681 atacataaac ttattgcaaa aataaaaacc gaaaacatca caatcatttt tttcatcata
    10741 tcacaacacc tcttaacaag gtaatatctc atatattatt ctattacccc ttttactaca
    10801 taaattatac tataaaagtg ttacaatact gttacatcgc atttaaaatt cagttacata
    10861 tgggcagtct tacagtaacc tcagtccctt tccccaattc acttgaaacg gatatcttgc
    10921 cgttgtgtgc ctcaactatt tcctttgcaa ttgcaagccc gagtcctgtc ccccccattt
    10981 ctctggaacg tgccttgtca gttctgtaaa atctctcaaa aatcctggaa gtatcctctt
    11041 tgggaattcc gattcctgaa tccgccacct taacataaac atcgccatac atctttccga
    11101 tatatattgt tatttttccc tccacaggag tgtactttat tgcatttgta aggatattca
    11161 aaaccacctg ttctatgcgg tccttatccc ctactatatt tgttatttca ccgatggtaa
    11221 agcactccag tgtctggttc ttttcttttg atgagaacct gaccttctcc acacagcttt
    11281 ttaccagatc ttcaaacgaa aattcctgca tgttccactt catttgtcta ttatccagcc
    11341 tcgagagctg aagaagatcc ttaaccagcc tggtcatcct gtctgcttcg gaattgatta
    11401 caccgagaaa cttttcggca gcttccctgt cctccaaggc cccgtccaat agtgtttcgg
    11461 aataactctt gatcgaagtc aaaggcgtcc tgagttcatg agaaacattt gcaacaaact
    11521 cctttcgcat gttctcaagt ttttgttctt ccgttatgtc gtgcagaacc gctattatac
    11581 cctcagcatt gttttccttg tcggtaaaca atgcaaaata catcctcacg aatttctctc
    11641 cgatatctat aactacctct tttgtgctat atttttcaag gtactgtatc tcctcaatcg
    11701 aaacccccag ttcgtacttt tttgagaatt cattaaaatc aaaggaaaaa tcatctattt
    11761 ccaatattct cttggcagca gggtttatat gaataacctc tccgttaata tcaaaggcta
    11821 ttacgccgtc ggtcataaag tttaatatgg tttctatttt gctcttttca cgggaaatct
    11881 ggttcaaagt gttttttagc tctctggcca taaaattgaa tgtctttgta agcttcccga
    11941 tctcatcctc cgactttacc tccaccatct ggtcaaaatc cccggctgcc atctttcttg
    12001 ccttatgcat aaggttgact atagggacag ttatcgtctt tgaaagggca taaccgaaaa
    12061 tcagcgataa aataactgca ataaccatgc tcagcttaat tatgttatta aaagcctcca
    12121 tcgctcctgc ccattcttcc ctgtcatacc tgaaatacag aacgtaatcg gagtttccta
    12181 tcctccgggc ataatcaaaa aaggccttct cattgattct tattaatttt ccccggtctc
    12241 ccgccttacc ggccaacact gcaaggtagt ttctggagtt ttgtatgtca tgagccaatg
    12301 tttccctata gggcatcctc tcgtcggtat acaaaatttc attggttttc atgtcagcta
    12361 ctgtaaacga tcggaaattg tttattgaaa acaaggacat agcattagcc ttgttatttg
    12421 cggtaagata ctcaagaatc tcttcttttt cagggctggc attgttgtct atgccccaat
    12481 attcaaaccc gctctcaata ccggctttaa acgtatcata ataaaaggtt tccacaaaat
    12541 agtttaatga tatgcctgca gaaatcatca aaacgaatgt cagcaatata aatattgtga
    12601 ccagtctcca ctgcaggctt cttaatagat tcagtcgccc aaaaatcata cctcatcctc
    12661 acaccaataa gcataaacaa ggaatgttta tataactaac tcaccttgtt aaaataatac
    12721 cctacgcctc ttttagtcat aatataactt gggttgctcg gatccttctc aattttctcc
    12781 ctgactcttc gcactgtaac atccaccgta cggacatctc cataatattc atagccccat
    12841 accttttcta aaaggctctc ccttgaaaaa atctgtccct gctggagggc taagaattta
    12901 accagttcga attcccttag ggtcagctcg attacattgt tatccctttt tacttcatag
    12961 tgctcaatat caatctccag gtccccaaac tttaaataat tgttcggctt cttttgtaaa
    13021 tccgacgaat ccacccttct caagtttgcc ttcaccctgg ccattaactc cctggtactg
    13081 aaaggctttg ttatatagtc atcggcaccc aattccagcc ccaggacctt gtcaacttcc
    13141 tcttccttag ctgttatcat tataataggc gtagaaattg tttcgcgaag ctttctgcat
    13201 acggtaaaac catccatttt aggcagcata atatcaagca atatcaaatc aggcttttca
    13261 ctgtgggcca attcgatagc ttgctcgccg tcataggcct caatacttga aaacccctct
    13321 tttgttaaat taaatttaag aatatctaca atgttttttt catcgtcaac aataagaatt
    13381 ttcgcactca caaaaaacac cccttaatta atgatagcaa gaagtcttct tcatcacact
    13441 taccattata acattaaata caaaaaaaag aacatatatt ttacgtcaat tactccattt
    13501 tattcaataa taaattttga ggtccagttt tccaaaatat tattatatat tattatacaa
    13561 acaatgttga tattattcca atttgtgtta taatataagc aatggttgtc ttattatgtt
    13621 attctgtttt agcaagtagc tttcttggaa tgcatcaaaa ctatatcaaa gggggctgca
    13681 agccatgtca aaggacttat tgatggttgt cattaccaaa atgcacatca ggcttaacga
    13741 attaatcgaa aaaaacgact atgatctttt atgtgaagag gttcaacttt atagcaggcg
    13801 gttggataaa tttctatcct actactataa agcagatgaa aaaaccccga gttttgaata
    13861 cgacttccgc gtacttgata ataccgtcaa atgctcagtc tgagcataat aaagacattt
    13921 gatacataac ataatcagat tttaatatta atttagggg
//
LOCUS       DAOOJC010000008        13677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_4230_length_13677_cov_3.815372, whole genome shotgun sequence.
ACCESSION   DAOOJC010000008 DAOOJC010000000
VERSION     DAOOJC010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 13677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13677
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4230_length_13677_cov_3.815372"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1588
                     /locus_tag="PLA01_00510"
     CDS             <1..1588
                     /locus_tag="PLA01_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPC99813.1"
                     /translation="VAWGDNRFEKCDVPDGLTDVIDIAAGESHSVAVKADGTVVAWGN
                     NDHRQCDVPEGLTDVCMVNSYWDHTLAVKNDGTVTGWGLNNWGQGTVWDDFTGVTSAA
                     AGAFFSLVLKEDGTVGAWGWMEAKIRPFLADLGKIKGVAAGHEHVAVIKEDGTVAVWG
                     YNQFGQSIDPYSFTGIKDIESAFSHVVILKEDGTVLVWGDDGTAIIPITDEVEWNYIF
                     RPEGLTGIKAIDAGFWHILALTEENTVIAWGENLEGQCEVPEGLEDVKQISAGSDHSA
                     ALKNDGTIVMWGSNEFGQCDVPEGLSGVKNIEMGAFHSVALLEDGTVKAWGFNNDGQC
                     NVPENLTEVIDISAGENCTLALKKDGTVVAWGGGSRGYFVPEDLKGVKELETKNNHTI
                     ALKEDGSIVVWGLNLNGQCNVHEDARTGVAQIAAGGLFTTVLKKDGTMHSWGAYNIKL
                     VGEQSKLGDLDGDGSITSTDITYLKRYILKQIKDFPVPNDLYVADLDSDGSIDSYDLT
                     LMKRCILKGYDSIEEFLASI"
     gene            complement(1626..2261)
                     /locus_tag="PLA01_00515"
     CDS             complement(1626..2261)
                     /locus_tag="PLA01_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPC99814.1"
                     /translation="MCGRYVLYSDNEFEELKEIVAETEKEINLKVGEVFPTNTVPVVV
                     ENRIQLLKWGFYTYDKKLIINARSETVESKPMFKNAFYGKRCLIPATSYFEWKKESSG
                     KTKYEISPANRSLFFMAGLYNTFRDNNNNVFDGFVIITTLANKTTSEIHSRMPAILER
                     GEEKLWLDKNITDTTLLKQILKPYSDESILCQVAQPSGSIETPPLFDHLTS"
     gene            complement(2369..3388)
                     /locus_tag="PLA01_00520"
     CDS             complement(2369..3388)
                     /locus_tag="PLA01_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPC99815.1"
                     /translation="MAKKVTMEFIAQKLGITKNTVSLALRNMPGVSQKTRNEILQMAE
                     KYGYKYKKPIHNNGCSKTESICLMLSNHIRNSVGFFSFIQYGIESEAKRNNLNTILYY
                     FDDNKEFIPPVCIKDGIVSGIVTLGPISRKTVTSILDLNLPVVIIDDFFDDIKASYIL
                     TDNLAGGFLATEYLIKSGHREIGFLGNIFAAPSFCDRYVGYLKAMSLYRIPVNNSYSI
                     LDKNMAFIFDECPDKAIEEVKKIPKLPTAFFCCNDSEAIHLYKIFSSMGVSIPEDVSI
                     IGFDDIESSKDISPELTTMHIYKEAMGERAVKKLIDRINGEKLMEEKILLPTVLVERQ
                     SVKRL"
     gene            3645..4685
                     /gene="hydE"
                     /locus_tag="PLA01_00525"
     CDS             3645..4685
                     /gene="hydE"
                     /locus_tag="PLA01_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HPC99816.1"
                     /translation="MINLIDKLSTEHCLSYGELHELIEHRNEETADYLFEKARQVRIL
                     HYGHDVYMRGLIEFTNYCRNDCYYCGIRKSNCNADRYRLTKEQILDCCAAGYELGFRT
                     FVLQGGEDGYYSDKTIADIVSSIKANHPDCAVTLSLGERSYESYKMFYEAGADRYLLR
                     HETANEEHYSKLHPPVLSLKNRKQCLYNLKEIGYQVGCGFMVGSPFQTTDCIVDDLMF
                     IKELRPHMVGIGPFIPHKDTPFADKASGTLELTLFLLGIIRLMLPGVLLPATTALGTI
                     HPKGRELGILAGANVVMPNLSPTDVRKKYLLYDDKICTGDEAAECRRCLERRIESIGY
                     KLVVSRGDCKFN"
     gene            4814..5734
                     /locus_tag="PLA01_00530"
     CDS             4814..5734
                     /locus_tag="PLA01_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPC99817.1"
                     /translation="MIKLLIKWYVKDYRNITDPKVRESYGVLSGVLGIFCNLFLFIVK
                     ISTGLLINSIAVISDAFNNLSDLGSSLISILGVKLSSRPPDSEHPHGHGRYEYITSLA
                     VSFVIFGVGLELLRNSFQKVMKPEEVIFSPISIILLVVSVFVKLWMFSYNRYIGKAIN
                     CGINKATASDSLSDVIATSAVIAGTLLGRVVNFPFDGVLGLVISVLIMNTGFGIAKDS
                     VDLLLGSSPDPELIDSINSYVLRGKGIKGVHDLKVHDYGPGKISASIHAEVSEGANIV
                     EIHSQIDEIEQRIKSELGVDIVIHMDPVEE"
     gene            5928..6740
                     /locus_tag="PLA01_00535"
     CDS             5928..6740
                     /locus_tag="PLA01_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HPC99818.1"
                     /translation="MSTFNRGSVVVRKSHGQDIYFTVIDVQNKDSAKPLYILRGMFFR
                     IIADAYEDDLIKKDYRNAIMDLRTHFVRAKSNAIRGLNFNRFLLNKAKRSSGRILHID
                     SSERFLDMCRNFYREAGLKSTGYLANESEQPNIIRRALLQSKPDILVVTGHDGIKKSN
                     SDISSIENYRNSKYFIECVKEARRYEPDYDKLCIFAGACQSHFEAIMESGANFASSPG
                     RININALDPAIVSEKVALTDRNSFVTPEEIGEITVSGRKGIGGIKTRGHLAN"
     gene            6940..8919
                     /locus_tag="PLA01_00540"
                     /pseudo
     CDS             6940..8919
                     /locus_tag="PLA01_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
     gene            9331..>13677
                     /locus_tag="PLA01_00545"
     CDS             9331..>13677
                     /locus_tag="PLA01_00545"
                     /inference="COORDINATES: protein motif:HMM:NF019161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPC99819.1"
                     /translation="MVKFRSIAKIVVMLMVISLCNIPVLASSNYSDTAGHWAVKAIDR
                     WSDYGVINGYQGQFRPNDPITRAEMAAVLDKIMKYRAKADNDFSDLDENWYTDSILKV
                     NFAEVMVGSDGKVRPKDNITRQEAVVMVCKALDIPESNTPSGFVDSDEIGAWALGYVN
                     AMANLGYISGVGGNRFAPRDNITRAAVVTILDNVIKALYQEEGTYTGNVDGVAIVSSG
                     GTELKDMNITGDIIISEGVGDGEVVLDSVKTEGTLIVRGGGVNSIIIKNSSVIPKVLV
                     NRRDGVVAVKVEGNSAEVGHIAVLSDGAVITAPMATVAVAEGVTGTLVNGIPVAPGQT
                     VLASNDPEDDVDVTPSIPVPVPTDTGTDSSSSSGSGSSTPRPTTSPQNNIITSIVLNS
                     EAKQMIVGEYFELIPYLEPYGVETQINWTSSDSDIAYVNSEGFVVASSIGTATITART
                     PNNVTAACFIVVSEPESGSIEINYPGTVINGGIYNNVTIGEDVGEGEATLNDVTITGT
                     LTINGGGSSSIHFNGNSQANNVEVNKAEGEKPRFLMNGTSSVANFNVSGESGAILERG
                     QNATGIVSRVVASRPLTIKGADVPEIETSSELELHNAQAVKVATTGETATIKLKGETR
                     LNKLEAGTQTRIESESTGSSIDEISTEDADVETDENSQVGSIIASGSANITVNGTANH
                     IETVGGNVSVTGNSSPVISGSPGTVSVGGNAKPVIAANATAVEIHAGGGAGITVTGSV
                     GSINTTGSGSIGSINIESGSPTVSIAGEVEVEVIAVSGAAAPQISGEEGSNPIINTIV
                     STSSNTVTVNVAASTVIAQDPEKIEGDGVETETVTQATPELNSITLTSLPRKLIYSED
                     DAELDTTGMVVTGNYTVSGVNVKKVLSELDYTVSGWPEDVSAGEHTITVTETESNKSA
                     AFDIKLVAKVVESISLKALPTKLIYEVDDEFSLAGGELTVYYTNKALYPNMSITLSDE
                     GVTVTGYTATVGVKTITLEYEGKSVRFNVTVKDSEAEALAKAKLDAEIELREFTAEIY
                     NTNKQNANSDSMKTAIIAIRDEAIQQIYDADNEEEINQIKEDAINQIEQVAMDELGNI
                     SILVGNAFFSDLQEAIDAATNSQIVEIVKDYWVEGEDVTIPYDKKVVNRASLTIENAT
                     LSVENGGLLTNEGDIMVFDVMGFNSCLSIEEGGTLNMETDSYIMTFHGGDNIRLDVQG
                     KLNAQQGTFIIVLGGSVTGIDGIETGEGQDGSGVYRYDDYSWNKMQSPDLGGFEVSIN
                     SDGDIVIRCKAWTEDIDVQVDNESKEFISKINDEYIVEGSYADGEPHNVIITVDGKWD
                     LIATALYIDSGTLPDLSELNGSGSFIYYSGGTFVTVAGDVTVSGGELHIPERVTVDVI
                     SGGSIDLTGKELTIDRTFEFWAGGGKSELVIRHGGSVTLKDGTTIGNGDSADVVLGAN
                     SNFKLSVYEDFSE"
BASE COUNT         4304 a         2333 c         3321 g         3719 t
ORIGIN      
        1 ggttgcctgg ggagataaca gatttgaaaa atgtgatgtt cccgatggac ttactgatgt
       61 aatagatatt gcggcgggtg aaagccactc ggtagcggtt aaagctgacg gaactgttgt
      121 tgcttggggt aacaacgatc acaggcaatg cgatgttccg gaagggctta ccgatgtatg
      181 catggttaat agttattggg atcatacgct ggctgtaaaa aacgatggaa ctgttacagg
      241 atggggactt aataactggg gtcaaggcac tgtatgggat gactttacag gtgttacatc
      301 cgctgcagcc ggagctttct tttcgctggt actcaaagaa gacggaaccg ttggtgcctg
      361 gggatggatg gaagcaaaaa tcaggccgtt tttggcagat ttgggcaaaa taaagggagt
      421 tgcagcagga catgagcatg tagctgtaat aaaagaggac ggaacggttg cagtatgggg
      481 atataatcag tttgggcaga gtattgatcc ttactccttt acaggaataa aggatattga
      541 atctgctttt tctcatgttg taattctgaa agaggatggc acggttttgg tctggggtga
      601 tgacggtacg gcaattatac cgattaccga tgaagtggaa tggaattaca tattcaggcc
      661 ggaaggatta acggggataa aggcaataga tgcgggattc tggcatatat tggcacttac
      721 ggaggaaaac actgtaatag cctggggaga aaacctggag ggacagtgtg aggttcccga
      781 aggcttggag gatgtcaaac aaatatcggc tggaagcgac cattcggcag cattaaaaaa
      841 tgacggtaca attgttatgt ggggaagcaa cgagtttgga cagtgcgatg ttccggaggg
      901 gctttccggc gtaaaaaata tagaaatggg agcttttcat tctgtggcat tattggagga
      961 cggaacagtt aaagcttggg gattcaataa cgacggacag tgcaatgtac ctgagaatct
     1021 gaccgaagta atagatattt cagccggtga aaattgtacc ctggcattga agaaagacgg
     1081 cactgttgtg gcttggggcg gtgggagcag aggttatttt gttcccgaag acctaaaagg
     1141 tgtaaaggaa ttggagacaa agaataatca cacaatagca ctaaaagagg atggttccat
     1201 tgttgtctgg ggattgaacc taaacggaca atgtaatgtt catgaggatg ccaggacagg
     1261 agtcgcacag attgcagctg ggggcttgtt tacaacagta ttgaaaaagg acggtacaat
     1321 gcattcctgg ggagcgtata atatcaagct tgttggagaa caatccaagt taggagatct
     1381 tgacggtgac ggaagtatta catccactga tattacatat ttaaagaggt atatattgaa
     1441 gcagattaag gattttccgg tgcctaatga cttgtatgta gcggaccttg attctgacgg
     1501 cagtattgat tcatatgact taacgcttat gaaaagatgt attttaaaag ggtatgacag
     1561 cattgaagag tttctcgcat cgatatagtt atgcgtgagg gcagtaacaa acggacagcg
     1621 ggatttcacg aagtcaaatg atcaaaaagc ggtggggtct caatgcttcc ggaaggctgt
     1681 gcaacttgac agaggatact ttcgtcggaa tatggcttta gtatttgttt taaaagcgtg
     1741 gtgtctgtta tgtttttgtc cagccagagc ttttcctcgc cgcgttctaa aatagccggc
     1801 attctggaat gaatttctga agttgtttta tttgccagtg ttgttatgat gacgaacccg
     1861 tcaaaaacat tgttgttgtt atcccgaaat gtgttataca gccctgccat aaagaataaa
     1921 gatctgttgg cggggcttat ttcatatttt gtttttccgg aagactcctt tttccattca
     1981 aaatatgagg tggcaggaat caaacatctt ttgccgtaaa atgcattttt gaacatgggc
     2041 tttgactcca ccgtttcgct gcgggcattg attataagtt ttttgtcata ggtataaaag
     2101 ccccatttca aaagctgtat cctgttttct actacaacgg gaacggtatt tgtgggaaaa
     2161 acttctccaa ccttgaggtt tatttccttc tccgtttcgg ctactatttc cttgagttcc
     2221 tcaaattcgt tatcggagta taaaacatat cgcccgcaca tatttgccag ctccttttgt
     2281 aattatgaaa aaagctatat tccatagttg aaatgctgtt gatgcaatta atttactgta
     2341 aatgaaaata tattctgaaa ccgaaatgtt ataaacgctt taccgactgc ctttcaacaa
     2401 ggacggtagg cagcagtatt ttttcctcca tcagcttttc cccgtttatt ctgtctataa
     2461 gctttttgac tgcccgttcg cccattgctt ccttgtaaat gtgcatggtg gttaattccg
     2521 gggaaatatc ctttgatgat tcaatatcgt caaaaccgat tattgaaaca tcttcaggta
     2581 ttgatacacc cattgaggaa aagattttat acaggtgtat ggcttcactg tcattacagc
     2641 aaaagaaagc agtaggcagt ttgggtattt ttttaacttc ttcaatagcc ttgtcaggac
     2701 attcatcaaa aatgaaagcc atatttttat ccagaatcga ataagaatta ttgactggaa
     2761 tccgatacag ggacatagcc ttcagatacc ctacatacct gtcacaaaag cttggtgcag
     2821 caaaaatatt tcctaaaaac ccgatttctc tatgccctga ttttattaga tactccgtcg
     2881 caagaaaacc tccggcaagg ttatcggtga gtatataact ggctttgata tcatcaaaaa
     2941 aatcgtcaat tataacgact ggaagattca ggtctaaaat ggaggttaca gtctttcgtg
     3001 atatgggccc gagagtaact attcccgata caattccatc ctttatgcac acaggaggaa
     3061 tgaattcttt attgtcatca aaatagtaca aaatggtatt gagattgttt ctcttggcct
     3121 ccgactctat accgtattga atgaatgaaa agaatcctac ggaatttcga atatgattgg
     3181 aaagcataag acaaatgctt tctgtttttg aacaaccgtt gttatggata ggctttttat
     3241 acttatatcc gtatttttcg gccatctgaa gaatctcatt tctggttttt tgactaacac
     3301 cgggcatatt cctcagagcc aaagagacgg tatttttagt aattcccaat ttttgggcta
     3361 taaattccat agttactttc ttagccattt ttccctccaa ataatagctt cactaattgt
     3421 tattataaat ataataatcg ggattttcaa cttaatttat aattacgggt gcattaaatg
     3481 aatataaatg tgaggaaaag tattgatttt tttttaaagg ttaaaaaaca tggcaaatat
     3541 gatataatga gttcagcagt atatgttgtt tgtaaaacta tgctcacaga aattatttac
     3601 aacactaaac cggtattgta caggtaaagg ttaagggatg ctatatgata aatttaattg
     3661 acaagctttc aactgaacat tgtctttcct atggcgaact gcacgagctg attgaacacc
     3721 ggaatgaaga aacagccgat tatttgtttg aaaaagcaag gcaggtgcgt attcttcatt
     3781 atggacatga tgtttacatg cggggactta tcgagtttac gaattattgc aggaatgact
     3841 gttattactg cggaataagg aagagcaatt gcaatgccga cagataccga ctcacaaagg
     3901 agcagatact tgactgttgt gcggcaggat atgaactggg cttccgcacc tttgtgcttc
     3961 aagggggaga ggatggttat tattccgata aaactatcgc tgatatagta agcagcatta
     4021 aggcaaatca tcctgactgt gctgtcactc tatcccttgg ggagagaagc tatgagagct
     4081 ataaaatgtt ttatgaagca ggagccgaca ggtatcttct ccgtcacgaa acggcaaatg
     4141 aagaacatta ttcaaagctt catcctccgg ttttgtcact gaaaaacaga aaacaatgcc
     4201 tatacaactt aaaagagata ggctatcagg tgggatgcgg ttttatggtg ggctctcctt
     4261 ttcagaccac ggactgcatt gttgatgatt tgatgtttat aaaagaatta cggcctcaca
     4321 tggtaggcat aggcccgttt attcctcata aggacacacc ctttgcagac aaggcttcgg
     4381 gtactttgga attgacattg ttccttcttg gaatcatacg ccttatgctg ccgggcgttt
     4441 tgcttcccgc aactactgcc cttggtacca tccatccaaa aggccgggaa ctgggaattc
     4501 ttgccggggc taatgtagtt atgccgaatc tttcaccgac agatgtcaga aagaagtatc
     4561 ttttatatga cgataaaatc tgtaccgggg atgaagcggc tgaatgcaga agatgccttg
     4621 aacgccgcat tgaaagtatc ggttacaaat tagttgtttc caggggcgat tgtaaattta
     4681 attaaataaa caaatggcac atctatggga cagtaagtat tccatgggtg tgttttttac
     4741 actcttaaga aaatacttat taagcgggga acaatttggg tgtcattgag aaaacgggaa
     4801 tggagggctg attttgatta aacttctgat taaatggtat gtcaaggatt accggaatat
     4861 aaccgaccct aaggtaagag agtcttatgg agtattatcg ggagtattgg gtatattctg
     4921 caatttgttt ttatttattg tcaagatatc caccgggttg ttaataaaca gtattgcggt
     4981 aatttccgat gcctttaaca acctcagtga cctgggctca tcattgattt ccatattagg
     5041 ggtcaagctc agcagccggc ccccggacag cgaacatccc catggtcatg gcaggtatga
     5101 gtatataaca tctctggcgg tgtcgtttgt tattttcgga gtgggccttg agcttctaag
     5161 aaattcattt caaaaagtaa tgaaacctga agaagttata ttcagcccca tatccattat
     5221 cctgctggtt gtttcggttt ttgtaaagct atggatgttt tcatacaaca ggtatatcgg
     5281 caaagcaata aattgcggga tcaataaggc gacagccagt gacagcctta gtgatgtcat
     5341 tgctacgagt gcagtaattg caggaacctt gctggggagg gtcgtcaatt ttccctttga
     5401 tggagtttta ggccttgtta tttcagtatt aatcatgaat acagggtttg gtattgcaaa
     5461 ggattcggta gacttgcttt taggttcatc tccggatccg gagcttattg acagcataaa
     5521 ttcctatgtt cttaggggga aaggcattaa aggtgtccac gatttaaagg ttcatgacta
     5581 tggacccgga aagataagtg catccataca tgccgaagtt tccgaaggtg caaacatagt
     5641 tgaaatacat tctcagattg atgaaatcga gcagagaatt aaaagtgagc ttggagttga
     5701 catagtgatt catatggacc ccgtcgaaga gtaggaatgg cacaagggga ggcagcacaa
     5761 ggggacagca caaggggacg gttcttccgt gtcaaatgac acggaagaac cgtccccttg
     5821 tgcggaagaa ccgtcccctt gtgctgttaa ttgtttaaaa aaatataccc ctaaagaggt
     5881 ttaattgcat attatgcaat atgcaaaatc tttttggggg gattattatg tcgacattta
     5941 atagaggaag tgttgtagtc aggaaatcgc acggccagga tatctatttt acagttattg
     6001 atgtacagaa taaagattct gccaagcccc tgtatatttt aaggggtatg tttttcagga
     6061 taattgccga tgcttatgaa gatgacctga taaaaaagga ttacagaaat gccattatgg
     6121 atttgcgtac gcatttcgta agggcaaaga gcaatgcaat cagaggttta aatttcaata
     6181 gatttttgtt gaacaaagca aaacgaagtt ccggaagaat ccttcacatc gattccagtg
     6241 aaagatttct agatatgtgc agaaacttct acagggaggc cgggcttaag tccaccggat
     6301 atcttgccaa tgagagcgag cagcccaaca ttataaggcg ggcacttttg cagagcaaac
     6361 cggatatact tgtggtgacc ggtcatgacg gtattaaaaa gagcaacagc gacattagtt
     6421 ccattgaaaa ttacaggaac tcaaaatatt ttatagaatg tgttaaggaa gccagaaggt
     6481 atgaaccgga ttatgataag ctgtgcatat ttgccggtgc ctgccagtca cattttgagg
     6541 ctattatgga gtctggggct aattttgcaa gctctccggg aagaataaac ataaatgccc
     6601 tggatcccgc gattgtatcg gaaaaggttg ccctgacaga cagaaacagt tttgttactc
     6661 ccgaagagat tggggagatt accgtttcgg gaagaaaagg cataggagga ataaaaacga
     6721 ggggtcacct tgcaaattaa catttgaagc agcaatacct gttttcatat atgcagcaga
     6781 atattattta agtgaagtaa aatatggaag aaacattgtg aagcaagtta cagccgcctt
     6841 ttgatacgac gcggaaaggg agagcattgt acagtttact ttgttgaatc ccagggctat
     6901 ttactgtaaa ataatataca cagcttaaga tagagggaga tgcggttggg gaaaaaactg
     6961 tacattactg aaaaaccttc tgtggcagca agctttgcag aagttatggg aataaaaatc
     7021 ggaagacagg acagagaaag gggctttgcc gagtcctttg acacaatcgt gacatggtgt
     7081 tttggacatc ttgtcacatt ggctatgcct gatgcttatg atccggcata caggacatgg
     7141 agggtggagt cccttccgat aatacctgaa aaatacaagt acatagtgat tgaagacaat
     7201 ggggtaaaaa agcagtttga gaccgtaaaa aggttaatga acagggagga tgttgacctt
     7261 atatatgcat gtaccgacag cggacgggaa ggagaataca ttttcaggct tgtgtaccag
     7321 cagaccggat gtaggaaaga agcgaaaagg gtgtggattt cttcacagac tgaggaggct
     7381 gtccgggacg gcatacggaa tgcaaaggac atcagcgagt atgactccct ggcacaggct
     7441 gcatattgca gggcgaagga ggactggctt ttcggcatga acttcagcag gatttacaca
     7501 tgcatgtacg gtgcgaaggt ttcaagcctt gtaaaggaga gcaagtcatc ggttattgcc
     7561 atcggaaggg taatgacatg tgtgttgggt ttagttgtgg gaagggaaca ggagataagg
     7621 aactttgtgc caaagaagca ttatggaatt gaggcaagtt ttgtatccgc aaacagcggg
     7681 attgaatata aaggcaaatg ggtgccgaaa ggggaaaaag gcagtaaggc ttcggatatt
     7741 aaagcagaaa acaagcaaga cgtaaagtga aacatggtta agcagacgga aaacacagag
     7801 gaagaggaaa agtatataga aaaggaagag gcagagagtg ttattgaaaa gcttaaggga
     7861 cattccggca agataaagaa aattcaaata aagactaaaa aggaacagcc gccccttctg
     7921 tttaatcttg cggagcttca atcggaagca aataagaaat tcaagcttcc ggtggacaaa
     7981 acccttgaaa ttgcacagag cctttatgag aaaaagctga taacctatcc gaggactgac
     8041 agcagggttc tttcaacaga cgttcttacc gaaataccga aggttttgaa cgggctatat
     8101 aaatgtccgg catacaggga ttatgttttg agaataaagg attttggaga tattaaggtt
     8161 acaaaagcct caaagaggtt tgttgacaat gaaaaggtta cggatcacta tgccataata
     8221 cccacatatg ttactcctga tattgacaaa atgaatgatg cggaaagaaa cgtgtacaac
     8281 cttattgtaa aaaggttttt ggcgattttt tatcctcctg ccgttttcaa taccgtacag
     8341 gtcgagaccc aggtgaagga tgaaattttt gtgtccaatg ccaaaaccct taaagaagcg
     8401 ggatggaagg aagtttatga ggtaagtgcc gccaaagggg aagaggattt aactgaggca
     8461 ccgatacacc ttctggttaa aaatgaggaa agcaaggttt tgggatatga gcttgaagag
     8521 aaactgaccc ggcccccttc aagatatacc gacggttccc tgataatcac catggagaag
     8581 gcaggaaaat ttatagaaga cgaggaactc cgggagcaga ttaaaacctg tggaatcggc
     8641 acatccgcca cccgtgccgg aataataaaa aagctcaaag atataggtta tatcaaaata
     8701 aactccaaga cccaggtggt aacccccaca cctaaaggtg aagctattgt tgaggtggta
     8761 aaaaggactg ctaaagagct tttgagccca tcctttacag caagctggga aaaaggcctt
     8821 gcaatgattg aaaataagga aacaacgcgg gaaatattcg agcagaagct ttatgcctat
     8881 attgcaaaaa caatagaaaa ggtaaagaaa tcccattaaa aaaatacttc atatcattga
     8941 tatgaagcca gttataagga aataatgttt tgcaagaaaa accatgaaat catttttgag
     9001 ggatgtttta taagagttac aaatactgcc aggattgaaa aacttaccta ctactatttg
     9061 tattctgatg tttcgttaat atgcatgcaa aatcacaata caggttctgt atttatgaaa
     9121 cttaagtttc tttccaactt ttttatttcg ccggaaaaat gtcaataagg gtgcaagaag
     9181 cgttctgggg tatagtcttt tcttgataat aataaagatt ttaatattta tacctaatca
     9241 aatgtaatat tgtaaaaaaa tataccgata taattgttgc gattatctga ttatttaact
     9301 caactaccta tgaaggagga gacttgtctt atggttaaat ttcgaagtat tgcaaaaata
     9361 gtagtaatgc taatggttat aagcttgtgt aatattccgg ttttagcatc ttcgaattac
     9421 tcggatactg ccggacattg ggcagttaaa gctattgacc gctggagtga ttatggtgtt
     9481 ataaacggct atcaaggtca atttcggccg aatgatccga ttacaagggc ggaaatggcg
     9541 gcagtacttg ataaaattat gaagtaccgt gcaaaagccg ataatgattt ttctgacctt
     9601 gatgaaaatt ggtacacaga ttcgatttta aaagttaatt ttgcagaggt tatggttgga
     9661 agtgacggga aggtaagacc taaagacaat attacacgtc aggaagcagt ggtaatggtt
     9721 tgtaaggctt tagacatacc ggaaagcaat accccgtctg gatttgtcga cagtgacgaa
     9781 ataggtgcgt gggctttggg ttatgttaat gccatggcta atttaggata catttcagga
     9841 gttggcggaa accgttttgc accccgtgac aatattacaa gagcagcggt ggtaacaatt
     9901 cttgataatg tgataaaagc tttatatcaa gaagaaggta catataccgg taatgtagac
     9961 ggagtagcaa ttgtatcctc cggcggaact gagctgaagg atatgaatat aacgggagat
    10021 attatcatct cagaaggagt aggcgacgga gaagttgtat tagacagtgt aaaaacggaa
    10081 ggaactttga tagttcgtgg aggcggagtt aattcgatta ttataaaaaa ttcaagcgtg
    10141 ataccaaagg tattggtaaa tcgccgagac ggtgttgttg ctgttaaggt cgagggtaat
    10201 agtgcagagg ttggacatat tgcagttttg tcagacggag ctgttattac tgcccctatg
    10261 gcaactgttg ccgttgcaga aggtgtaaca ggcaccttgg ttaacggaat acctgttgca
    10321 ccgggtcaaa ctgttttagc ttcgaatgat ccggaggatg acgtggatgt gactccttct
    10381 ataccggtac ccgtacccac cgatacagga actgactctt catcaagttc gggatccggt
    10441 tcatcaacgc ccaggccgac aacaagcccc cagaacaaca taataacatc aattgttctt
    10501 aactcagaag ccaagcaaat gattgtcggc gaatactttg aacttattcc gtatttggag
    10561 ccttatggag tggaaacaca gattaattgg acaagcagtg attcggacat agcttatgtg
    10621 aattccgaag gatttgttgt ggcatcttcc ataggaactg ctacaataac cgccaggacg
    10681 ccaaataatg ttacggcagc ctgttttatt gtcgtatctg aaccagagtc cggatctatt
    10741 gaaattaatt atcccggaac cgttataaac ggcggaattt ataataatgt tactattgga
    10801 gaagatgtag gtgaaggtga agcaaccctt aatgatgtta caataaccgg aactcttaca
    10861 attaacggag gaggttcctc ttccatacat ttcaacggta attcccaggc gaataatgta
    10921 gaagtaaata aggcagaggg agaaaaaccc agatttttaa tgaatggcac ttcatcagtg
    10981 gcgaatttta atgtttcagg agaaagcggt gcaattcttg aaaggggtca aaatgctaca
    11041 ggcatagttt ccagggttgt ggcgagcagg cctttaacaa taaagggtgc cgacgtaccc
    11101 gagatagaaa ccagctcgga gttggagctt cataatgccc aagcggttaa agttgctaca
    11161 acgggagaaa ctgcaacaat taagcttaag ggagaaacca ggcttaataa gcttgaagcg
    11221 ggtacacaaa ccaggataga atccgagtca accggcagct caattgatga aatatctact
    11281 gaagatgccg atgttgaaac cgacgaaaat tcccaggtgg gatcgattat agcatcagga
    11341 agtgcaaata taaccgtaaa cggtactgca aatcatattg agacagttgg cggaaatgta
    11401 tcggtaacgg gtaactcatc accggttata tctggttcac ccgggacggt tagcgtagga
    11461 ggaaatgcaa aaccggtaat tgcagcaaat gcaactgctg tagaaattca tgcaggcgga
    11521 ggtgccggga taaccgtaac aggatcggtt ggaagcatta acacaacagg atcaggcagt
    11581 atcggttcaa taaatattga aagcgggagt cctacagtta gtattgctgg cgaagttgaa
    11641 gttgaagtta tagcggtctc aggggcagct gcaccgcaaa tttccggaga ggagggctcc
    11701 aatccgatta tcaatacgat tgtgagcact tcaagtaata cagtaaccgt taatgtagct
    11761 gcaagtactg tgattgctca agatcccgaa aaaatagagg gtgatggagt tgaaactgaa
    11821 actgttacac aggcaacgcc tgaattaaat tcaataactt tgacttctct gccaagaaag
    11881 ctgatatata gtgaagatga tgctgaatta gatactacag gtatggtagt aacgggtaac
    11941 tacacggtca gcggagtcaa tgtgaaaaag gtgttatcgg aattggatta cacggtttcg
    12001 ggatggccgg aagatgttag tgccggagag catacgataa cagtaaccga aacagaatcc
    12061 aacaagagtg cagctttcga tatcaagtta gttgcaaaag tggttgaaag tatttcgtta
    12121 aaagcattgc caacaaaact gatttatgaa gtcgatgatg aatttagctt ggctggagga
    12181 gagttaactg tttactatac aaataaagct ctttatccta atatgtcaat tacattgtct
    12241 gatgaaggcg taacagtgac cggttatact gctacagtgg gtgttaagac cattacctta
    12301 gaatatgaag gcaaatccgt tcggtttaac gtgactgtca aggacagcga agcggaagca
    12361 cttgccaaag caaaacttga tgcggaaatt gagttgaggg aatttaccgc agagatctat
    12421 aatactaata agcagaatgc aaacagcgat tcaatgaaaa cagcgataat agccataaga
    12481 gacgaagcaa tacagcaaat ttatgatgca gataacgagg aagaaataaa tcagattaaa
    12541 gaagatgcaa ttaaccagat tgaacaagtt gcaatggatg aacttggaaa tatatccata
    12601 ttggttggca atgccttctt cagtgatttg caagaagcca tcgatgcagc aacaaacagt
    12661 caaatcgttg agatagtaaa agattattgg gttgagggag aagatgttac aataccttac
    12721 gataaaaagg ttgttaacag agcgagtttg acaattgaaa atgcaacctt gtcggtggag
    12781 aatggaggct tgctgacaaa tgaaggagac ataatggtct ttgatgtcat gggatttaac
    12841 agctgtctta gtatcgagga aggcggcaca ttgaatatgg agaccgacag ctatataatg
    12901 actttccatg gcggcgataa tataagactg gacgtacagg gaaaattaaa tgcccagcag
    12961 ggtacattta taattgtatt aggcggcagc gttacaggaa tcgatggaat tgaaactggt
    13021 gagggacagg atggaagcgg tgtatacaga tacgacgact attcatggaa taaaatgcaa
    13081 agtcctgatt tgggaggatt tgaggtttca ataaattccg acggagatat agtaataagg
    13141 tgtaaagcat ggacggaaga tattgatgta caggtagata acgaaagtaa agagtttata
    13201 agcaagatca atgatgagta tatagtagag ggcagctatg ctgacggtga gcctcataat
    13261 gtaataatca cggtagacgg caagtgggat cttatagcta ctgcattgta tattgacagt
    13321 ggaaccttac cggatctttc cgaactgaac gggtcaggct cttttatata ctactcagga
    13381 ggaacatttg taacagttgc aggagatgtg acagtaagcg gaggagaact tcatataccc
    13441 gaacgtgtga ctgttgatgt tataagcggc ggaagcattg accttacagg taaagaactc
    13501 actattgatc gtacgttcga attctgggca ggaggaggca aatccgaact ggttatccgt
    13561 cacggcggaa gtgttacgct gaaggatggc acaactatcg gtaacggaga ttcggcagat
    13621 gtagtattag gagcgaatag caactttaaa ctaagtgttt acgaggactt tagcgaa
//
LOCUS       DAOOJC010000009        12446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_4677_length_12446_cov_3.664757, whole genome shotgun sequence.
ACCESSION   DAOOJC010000009 DAOOJC010000000
VERSION     DAOOJC010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 12446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12446
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4677_length_12446_cov_3.664757"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..720
                     /locus_tag="PLA01_00550"
     CDS             178..720
                     /locus_tag="PLA01_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99820.1"
                     /translation="MKFSRMLLTATIITIISSASAFAAPSAKDSGLSESNAVQQQNKE
                     CEKGHCTAKKKGDCEEKLKDPIEMLQRKKQKVQELYDGGKITKEEADELIKRIDAKIR
                     EIQEFNSLPLDKKKEKLLAGFKEKVESKVKEGKLTKKEADELIKKFTERLKEWDGKGY
                     PGYKSGKYREKGKGFFRKKQ"
     gene            1035..>12446
                     /locus_tag="PLA01_00555"
     CDS             1035..>12446
                     /locus_tag="PLA01_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99821.1"
                     /translation="MNRNLKVPISILLCFLMLFAEMMPVGILSAPVFAEGSVGDESLN
                     SAVNSETDGFPSSKSVTDSVYDAKEESEGETEEEPLSSVDTSEPVLVKSASSGVNEVL
                     NEYVISGTVYLPEGYVAPKGGLNLKVYAKSSKTNSVSLTIPEGKNKVEYSMIVPDAGS
                     YTLYYQVSSADYVSSGYYSESGTVRSSSSADTVKLSEGAPVQSDINLTLIENRIISGE
                     VFILDGVATAGGLSVTVKAECGSDKVTKTVTIPEGGNVADYELTVPPNIAGKGYLVSY
                     QTSDKAYLGTAYYHKDGSVRYNTLATLVDVSSGNKEGVDINLISMKKITGTVSLPSGT
                     APKNGVSVTLYAFSGSDKTSEAVTIPEGVSSIEYSLYVPEGTGYKVYYTTDNEAYFST
                     GYYNNNGMVKDSKAASVVNIISGDAENINIELIANRIVSGIVSLPGKKADQDISVEVI
                     VALNSNILKSTAIVIPAGGSQASYSMYVPTGSGYNVSYKTQNVSYVEQGYYKEGSTVR
                     YLSSASLISVENTDIKNIDLTLIEKRTISGVLSMPSGKAPRGGISFEITASKGSQQAK
                     TTVTIEEGTDSVPYSISVPADEGYIIKCKLLTLQVIYMNEQYYSSGGTVYNIDSASTV
                     STISGNQPNINIMLLEKRTVSGTLSFPSGFVAPEGGLVFTNETDGRITYIIFEEGASS
                     APFTIYYNPGKFYLYYDFGDDSIFVSPGYYSKGGTVIDESSADLIDVTEGNQVGIDLK
                     LLFKKTISGKVVLPKGVAPAGGYTVTVKASGSKGKTEQTVTINPGEKEADYTLFVNPG
                     DKYSVWYESGKKDNFVSPMYYNSDGMVRDQKNASLLDLRTENKTDIDLTLTEMRSVSG
                     SILLPSGVATGEGIDVDVVVSNGKDTTEVSVTIPGGKSVADYTAYVPAGKDYMVRYEV
                     NLKSSYATGGYYGVSGTTLNASSAGLLDLSLENKNEINMTLIPKRSISGSVSLPSGMT
                     VDSDTKVTVYAGNSYSASVTIPAKQTSVSYTVLVPPNSEGSGYKVYYKLTSDAMFISP
                     GYYSSSGMTAFEDSAELVDVSNSDSKADLILIPKSSISGMVKLPEGEIAPKGGLNVTV
                     TSSNSKNKGSVVVTVPEGKSSVPYTVYVPSGTGYLVEYKTSSEEYCQIGYYSNTGTVK
                     KADTATLLNVSEGSQKNIDIELIKNKKITGNFRLPKGVAPAGGFKANLVASNGTDLVR
                     KSVTIPQGYSSISYTLYVPEGKNYTVWYEATDRSVMPVGYYTDGGMTVDKSKAKLIDA
                     STDVSSINIDVIPKISLKGSVKLLSAPAPAGGISVKLTIDNKTSKDSLDVLIPEGFTS
                     APYELYVPAGEDYILQYTTSSTGFVSPGYYSESGTKEKYSEASLLNILKDREGLDIVL
                     ITKRTIRGMIMLPEGYAPTAGVSVKLTAQSAKDKITASFTIKEGENTVPYTLYVSSEQ
                     EYIVKYETADEKYVSLGYYSMSGTTREESKADRLDTTEADQVGVNIKLISNRYIAGII
                     SVPSGIAPFGGIKVVLRASNGKDSRETEVVIPEVSSSTAYRLYVPEGKDYELSYKIVN
                     PDGRYLPNGYYNADTATREREETVLIDLSRQSKEGMNITLIPNRLVTGSILLPSGVAP
                     SGGLKISVVAQNKRDSVSTQVIMPQGSSSIVYRMYLPEGEDYTIGYSMNHEDYMSGYY
                     NINDTVLSKAEATTFNVSKNDILGINITVIPKRTIKGVISLPDGKTAPAGGIDVTVSA
                     GSYKTKVTIPQNKSTALYTLKVSPNAFGSGYAVKYAISSSYGFVPTGYYGEEKTVAGI
                     QTASLVDVSLGNKTEIDLSIMDKRIIQGVFELGSGVAPAGGIDVTITAGGKSAEGKTV
                     SYQEVVNIPFGLNRTNFELKVDPSSFASGYYLKYTMDSVYGYAETGYYNDNGTVQNQS
                     EATLLNVYEEDQREKNFAAISKNHISGIVSLPEGMVAPRGGVKISVNAENGKDSGSVD
                     VTIPEGQVFTDYQLIVPPGTGYNLYYSMAPNNSYVTNGYYSNEGTVTDPKKAERFEMY
                     GNIEDKNIVLIAKRRISGEVLLPAGEFAPAGGLRVEVYANNGTISDSVTVFIPENGSS
                     QSFEIYMPPENGYKLYYTLSSGIDYVNKGYYASNAAVLEEREASVIDLRDKDFTDTTV
                     RLIKNSVLEGNVILPEGQAGEGGIEVRVTADNGKYSYSTDVLIPEGSSNKAYSLAVPP
                     ATGYTVSYQVSTGLDYKPKGYFSYEGTVSSIYEASKLNLSSKGEKGVDLTLIHYNSIS
                     GTISLPEGVAPDEGVTVTLFADNSRNKRQLTVTIPAENSSTNYNIYVPDGNGYKVYYV
                     MTPNEEYVDKGYYASNKTAVNEKDCVTLDVIGDSKYDTNLTIIAKRTISGTVSLEKGE
                     TAPQEGVSVRISAQEGDEQTVVIPYGKNSASYALKVVPKDLGYGYKVKYETLKNYGYI
                     RYGYYSEDGCVRNEAKADIVDVSDGDRENIDIEIVRPRTIEGKLRLPEGVAASKDMTV
                     SIIASNSLDSAEVSVYVPKGSSEALYSLNVPPNDKNDAYKVRYENWSDNSFADIGYYS
                     KSETVRNAGLAESVNVRNQNASGIVLTLIAKRNVSGKVSLPNGSAPAGGLTVTVYLEN
                     DTDKVVSYATILQNQSSADYSVNVPVGKNYKVGYEISTKNNLVPWGYYGAYGTVYMPD
                     SAYLLDVTDDIDGIDMTLLEKKSISGTVSLPEGKAPKGGVKVEIYAEDAGDTWVTIPE
                     GESSAQYSMKVVPSVEGKGYKVRYVVSQDHDLVNYGFYSKEGTVRNSKLADPVYANNK
                     DVTDINIEIMKPRKISGKVSLPEGVAPEGGISLNIAVTNDVDGNSQIIIIPEGESFAE
                     YSISLPPNDPGYEYKVRYENWSNKVFTTYGYFNSKGTTNNLSYAEPVDVNKTDANNVD
                     MVLLRKSTISGNFEVPEGCVLPEEGLHVRIYVSNDVETYSSNITVPYGTTSVPYSVHV
                     EEGSGYRLFYALDSNDRFMEYGYYGEDGVYTDKKRAKVFTVTDENISGCKLRLLEKRI
                     ISGKFTLPYDLDEVGSIEVSISASNGLDRGETKIRVPYGETEVDYTLNLPAGSGYILQ
                     YDIITIKGFASSGYYSEGGMVRSKDKADTLDLAENDLTGIYMSLIEDMTINGIIKLPQ
                     GVAPEGGIEVTVTATDESGNTSSCIRTIKEGENSVEYYLNVPPSNYNSGYKVRYSVTS
                     DDYATLGYYSESGTIVLPDLATPVDVSSENAEDINLMLIEKKVISGVVSIPGTVAKEG
                     GLAITIRASSQLFSVDDRMEVVIPEGESKAYYSLKVSPNVEGADYVIFYEVDDDAYVG
                     TGYYNTAGTVVDIKMATPVDVSNGNYSNADISLVRSRQISGSIALPDGETAPKGGVSI
                     TVFAEKTDYTGYRASKTILIPESKNWAEYTLIVPESTSKVVGLDIEASTDNGTGDTAD
                     DYTLSTKVYIPSASNNRNSDYMVGYSYVAGEKYFKSGFYSKAGTVPAKGMADILDAAG
                     KDIENIDFTLLKKDTTIKGTVKLPEGKTAPKDGITVSITAENDALDFVSEKTVVISSG
                     NSTVGYEIEVPSIDDYHIKYTIKSTGGYVTSGYYSEEGTVGTKERSTSINCSSGSVTG
                     INLNLIPGIEIRGKILLPSGHKVNIKDFWMWVVASNENYESTAYITISEGSSVANYSV
                     YVPEGSDYVVSYSLMPLFGEYIEKGYYNSSMTTANKNCATKLNVTKNISGIEMTLIPV
                     DRIVSGTVSLPDGTAAVPYSIRVPSNNRGNGYRVGYSVTSGNTDGLYNENGYFSPFGT
                     TTNINEASTIDVSIRNSSDRDMTLITADMKPVEAIMLDKYQVTLQSGG"
BASE COUNT         4103 a         2133 c         2927 g         3283 t
ORIGIN      
        1 cggttcaacg agagggaaat aattcttagc aattatttct ctactctatt ttattgtaaa
       61 taacggaagc ttccataaat gaaataaagt gcaatcaatt cacaaaatga acattaaaaa
      121 ttaaaattaa tttatgtaga ctggttatac taaatattaa ataaaggagg tgttcaggtg
      181 aagttttcca gaatgctgtt aactgctacg attataacaa tcataagttc tgcttctgct
      241 tttgcggctc cttcggcaaa ggattcaggt ttatccgaat ccaatgctgt gcagcagcaa
      301 aataaagaat gtgaaaaagg ccactgcacc gcaaagaaaa agggagattg tgaagagaaa
      361 ctgaaagacc ctatagagat gctgcaaaga aaaaagcaga aggtacagga actttatgat
      421 ggtggaaaaa ttaccaaaga agaagcagat gagctgataa agagaattga tgcaaaaatc
      481 agggaaatcc aagagtttaa cagtcttccg ttggataaga agaaggaaaa gcttttagcc
      541 ggatttaaag aaaaggtaga aagcaaggta aaagaaggta aattgacaaa aaaagaagct
      601 gatgagctta ttaagaaatt tactgaaaga ttaaaggaat gggatggaaa aggatatccc
      661 ggatataagt cgggaaagta ccgggaaaaa gggaaaggtt tctttagaaa aaaacaataa
      721 aattttcttt gaaggaattt aacataaaag ttggattcct tcaagcatac atatcttttt
      781 gcatttattt atgcatctaa taaatgagta aattaaagaa atcttgtata ctttcatttc
      841 atttatatga tataatattt aatggtgtta aagaatacat atatatcaca attattctaa
      901 ttagtgaatt tttttattcg ctttgggggg tatggtaaag gacaatagga ttatttaaca
      961 ttctatattc tatttatttc taaatatttt ttcatataga ttttacaaaa taattaaaat
     1021 ttggggggta atacatgaac agaaatctta aagtaccaat ttctattcta ttgtgtttct
     1081 taatgctgtt tgcagagatg atgcccgttg gaattttatc tgcaccggtt tttgcagagg
     1141 gttccgtcgg tgatgaatca ctgaattcgg cggtaaacag cgaaaccgac ggtttcccaa
     1201 gttccaaaag tgtaactgac agtgtttatg atgctaaaga agagtcggag ggagaaactg
     1261 aagaagaacc gctttcaagt gtggacacaa gtgagccagt ccttgtgaaa agtgcttctt
     1321 ctggtgttaa tgaagtttta aacgaatatg ttatttccgg tactgtgtat ttaccggagg
     1381 gttatgtggc acctaagggt ggattgaact taaaagtgta tgccaaaagc tcaaaaacca
     1441 atagtgtaag tcttactatc cccgaaggaa aaaacaaagt cgaatatagc atgattgttc
     1501 cggatgcagg aagttataca ttatactatc aagtatcgag tgcagactat gtaagctcag
     1561 gctattattc cgagagcggc acggttagga gttcttcttc ggccgatact gttaaattgt
     1621 cggaaggtgc tcctgtgcaa tcggatataa accttacact gatagagaac agaatcattt
     1681 cgggagaggt ttttattctc gatggtgttg caactgccgg cggattatct gtaacagtta
     1741 aagcagaatg cggctctgac aaggtaacaa aaactgtgac cattcctgaa ggaggcaatg
     1801 tcgcagatta cgaactgacg gtacctccca atattgctgg aaaagggtat cttgtcagtt
     1861 accagacatc ggacaaggct tatcttggta ctgcatatta tcataaagac ggttcggtga
     1921 gatataatac gctggctacg cttgtcgatg tcagttccgg gaataaggaa ggtgtcgaca
     1981 taaacctgat ttcaatgaaa aaaataaccg gaacggtttc tcttcctagt ggcacggcac
     2041 caaaaaatgg tgtatcggtt acattatatg cattcagcgg ttccgataaa acttctgaag
     2101 cggttacaat accggaggga gtttcttcaa tcgaatatag cctctacgtt cctgaaggaa
     2161 caggttataa ggtatactat actactgaca atgaagcgta tttttcaacc ggctattata
     2221 ataataacgg tatggtaaag gatagtaagg cagctagcgt tgttaatatt atctcaggag
     2281 atgcagaaaa tataaatatt gaacttattg cgaaccgtat tgtttccgga atagtatcgc
     2341 tgcccggtaa aaaagcagat caagatatat ctgttgaagt aattgttgca ttaaacagca
     2401 atatattaaa gagtaccgcc attgtaatac ctgccggtgg atcacaggct tcttattcca
     2461 tgtatgtacc gacaggttcg ggttataatg tttcatacaa aacccaaaat gtttcttatg
     2521 ttgaacaggg atattataaa gaaggttcta cggtcaggta tttaagttct gcaagtttaa
     2581 tatccgttga aaatacggat attaaaaata ttgacctgac acttattgaa aaaagaacta
     2641 ttagtggtgt tttatccatg ccttccggga aagcaccccg tggcggaata agctttgaaa
     2701 ttactgcttc caaaggttct caacaggcta agaccactgt tacaattgaa gaagggacag
     2761 acagcgttcc ctattcgata agtgttccgg ccgatgaagg gtacataatc aagtgcaagc
     2821 tgctaacact tcaggtaatt tatatgaatg aacagtatta cagcagcggc ggtacggtgt
     2881 ataatattga ttctgcctca accgtcagca caatcagtgg aaatcagccg aatattaaca
     2941 taatgctcct tgagaagaga actgtaagtg gaacattgtc tttcccgtca gggtttgttg
     3001 cacctgaagg cggtctggtt tttactaacg aaaccgatgg aagaataact tatattatat
     3061 ttgaggaagg agcgagctcc gcacccttca ctatctacta caatcccggt aaattctatc
     3121 tctactatga ctttggagat gacagtattt ttgtatctcc cggctattac agcaagggtg
     3181 gtactgttat agatgaaagc agtgcagatc tcattgatgt taccgaagga aaccaggtag
     3241 gcatagattt gaagttgtta tttaagaaaa ccatttcggg aaaagttgtt ttgccgaaag
     3301 gagttgcacc tgccggaggt tatactgtga cggtaaaggc atcaggaagc aaaggaaaga
     3361 cagaacagac tgttacaata aatccgggag aaaaagaagc cgactatacc ctgtttgtta
     3421 atcccggaga taaatacagt gtatggtatg agtctggcaa gaaggataat ttcgtaagtc
     3481 ctatgtacta taattccgac ggaatggtga gggatcagaa gaatgcatcg cttcttgact
     3541 taagaacgga aaacaaaacc gatatagact taaccctcac agaaatgcgt tctgtcagcg
     3601 gaagtatact tcttccttcc ggggttgcga caggggaagg aattgatgtg gatgttgttg
     3661 tttccaacgg aaaggataca acagaagtat ctgttacaat tccgggggga aagagtgtag
     3721 cagactatac cgcatatgtt cctgcgggaa aagattacat ggttagatat gaggtgaatt
     3781 taaagagcag ttatgctacc ggcggctact atggggtgag cggtacaacc ctgaatgcaa
     3841 gttctgccgg attacttgat ctgtctttgg aaaacaaaaa tgagattaat atgacactga
     3901 tacccaaaag gtcaataagt ggttcggtgt cgttgccttc gggaatgact gttgacagtg
     3961 atactaaagt gacagtttat gctggaaaca gctattcagc ttcggttaca atacctgcga
     4021 aacaaacctc tgtctcttac actgtgctgg ttccgcccaa cagtgagggc tcggggtata
     4081 aggtatatta caagcttacc tcagatgcaa tgttcatttc accgggatac tatagcagca
     4141 gtggaatgac ggcgtttgag gatagtgcag agcttgtgga tgtaagcaat tcagacagca
     4201 aagcagacct tatactgatt cctaaaagca gtataagcgg tatggtaaaa cttcccgaag
     4261 gggagatagc tcctaagggt ggtttaaatg ttactgttac ttcatccaac tccaaaaata
     4321 aggggtctgt tgttgtcact gttccggaag ggaagagttc tgtaccatat actgtttatg
     4381 ttccgtcggg gacagggtat cttgttgaat acaaaacaag cagtgaagag tattgtcaaa
     4441 tcggttatta cagcaataca ggcactgtta aaaaagcgga tacggctacc ttgctcaatg
     4501 taagtgaagg cagtcaaaaa aatatagata ttgaacttat taagaacaag aaaataaccg
     4561 gaaactttag acttccaaag ggagttgcac ctgcgggagg atttaaagct aaccttgtgg
     4621 caagcaacgg aactgatttg gtaagaaaaa gtgttacaat accacagggt tacagttcca
     4681 taagctatac actgtatgtt ccggaaggga aaaactatac agtatggtat gaggcaacgg
     4741 acaggagcgt tatgcctgtc gggtattaca ctgacggagg aatgactgtt gataaatcca
     4801 aagcaaaatt gatagatgct tctaccgatg tatcatcaat aaacattgat gttataccga
     4861 aaatttcttt gaaggggagt gtaaaacttt tatcggctcc cgctccggca ggtggaatca
     4921 gcgttaaact gacaatcgac aacaaaacct caaaggattc gttggatgta ttgatacctg
     4981 aaggatttac ctctgcaccc tatgagcttt atgtgccggc aggagaggac tatattcttc
     5041 aatataccac atcaagcaca gggtttgtaa gtcccgggta ttattcggag tcgggtacga
     5101 aggaaaaata cagtgaagct tcattattaa acatattgaa agaccgtgag ggacttgaca
     5161 ttgtgcttat taccaaaaga accattcgcg gtatgataat gcttccggaa ggatacgctc
     5221 cgacagccgg ggttagtgtc aaattgactg cccaaagtgc aaaggacaaa attactgcga
     5281 gctttacaat aaaagaggga gaaaacactg taccgtatac attgtatgta tcttccgagc
     5341 aggagtacat tgtaaagtat gagactgccg atgaaaaata tgtgagcctt ggatattaca
     5401 gcatgtccgg cacaacaagg gaagagtcga aggctgaccg gttggataca accgaagcag
     5461 accaggtggg tgtaaacata aagctcatca gtaacaggta tatagctgga attatatctg
     5521 ttccatcagg tattgcacct tttggcggaa taaaggtagt actcagagct tccaacggaa
     5581 aagacagcag agaaactgag gtggtaatac cggaagtcag cagttcaacg gcttacagac
     5641 tttatgtgcc cgaaggcaaa gactatgaac tcagctataa aatagtgaat cccgatggca
     5701 gatatttgcc gaacggatat tataatgcag atacggcaac aagagaaaga gaagaaaccg
     5761 tgcttattga tctttcacgt cagagtaagg aaggaatgaa tattacactg atacccaaca
     5821 ggctggtgac aggaagtatt cttttacctt caggagtggc accttccggt ggattaaaaa
     5881 tctcggttgt tgcacaaaac aaaagggatt cggtttcaac acaggtgatt atgccccagg
     5941 gaagcagttc aatagtgtac aggatgtacc tgcctgaagg agaagattat actatcggtt
     6001 attccatgaa ccatgaggac tatatgtccg gttactataa cataaatgat actgttttga
     6061 gcaaagcaga agcaacaaca tttaatgtaa gtaaaaatga tatcctggga attaatataa
     6121 ccgtgatacc taagagaacc attaagggtg ttataagtct tcccgacgga aagaccgcac
     6181 cggcaggagg aattgatgtt acggtttctg ccggaagcta caaaacaaag gtgactattc
     6241 ctcagaataa gagcactgca ttgtataccc tgaaggtgtc accgaatgcg tttggttcgg
     6301 ggtatgccgt taagtacgca atttcttcat cctacggttt tgttcccact ggttactatg
     6361 gagaagaaaa aacggttgcc ggtattcaaa cagcgtcatt ggttgatgtc agcctgggaa
     6421 ataaaacgga aatagatctc tcaattatgg ataaacgaat aatacaaggg gtatttgaac
     6481 ttggttccgg cgttgcaccg gcaggaggaa ttgatgtaac aataactgct gggggaaaat
     6541 cggcagaagg aaaaactgtt tcataccaag aggttgtcaa tatacccttt ggactaaacc
     6601 gcacgaattt tgagttgaag gttgatccaa gcagttttgc ttccggatac tacttaaagt
     6661 ataccatgga ttccgtttat ggttatgctg aaaccggata ctataatgat aatggaacgg
     6721 ttcaaaatca gagcgaagcg acattattaa atgtgtatga agaggatcaa agggaaaaga
     6781 attttgctgc tatctccaaa aaccatataa gcggcatagt atctcttcct gaaggtatgg
     6841 tggctccaag gggaggagta aaaataagtg taaatgccga aaacggaaaa gacagcggat
     6901 ctgtcgatgt tactatccct gagggtcaag tatttaccga ttatcagctt attgtacctc
     6961 ccggtacagg atacaacctg tactatagca tggcaccaaa caacagttat gtaaccaacg
     7021 gatactatag taatgaagga acggttacag atcctaaaaa agcagaacgt tttgaaatgt
     7081 atggcaatat tgaggataaa aacattgttc ttattgcaaa aagaagaata tccggagagg
     7141 ttttacttcc tgcaggcgaa tttgctcctg ccggagggtt gagagttgaa gtatatgcaa
     7201 ataacggcac aattagtgac tctgtgacag tgtttattcc tgaaaacgga tcatctcaaa
     7261 gttttgagat ttatatgccg ccggaaaacg gatataagct gtattatact ttatcttcgg
     7321 gtatcgacta tgtaaataaa ggatactacg caagtaatgc cgcagtgctt gaagaaagag
     7381 aagcatcagt aatcgatttg agagacaagg attttactga taccactgtc aggttaatta
     7441 aaaacagtgt attggagggc aatgtaattc tgcctgaggg tcaagccgga gaaggaggca
     7501 ttgaggtacg ggttacagca gataatggta agtatagtta ttcaaccgat gtattaatac
     7561 ctgaaggaag cagcaacaag gcatattcgt tagcggttcc gcctgcaaca ggatatacgg
     7621 tatcctatca ggtttcaacc gggctcgatt acaagcctaa aggttacttc agctatgaag
     7681 gaacagttag ttctatatac gaggcatcaa aattgaattt gtcatcaaaa ggagaaaagg
     7741 gtgttgatct gactcttatc cattacaaca gtattagcgg aacaatatcc ctgcccgagg
     7801 gggttgcacc ggacgaaggg gtaacggtaa cactatttgc ggataattcg agaaacaaga
     7861 gacaattgac tgtaacgata cctgccgaaa atagctcaac gaattacaac atttatgttc
     7921 ctgacggcaa tgggtataag gtctactatg tcatgactcc caacgaagaa tacgttgaca
     7981 agggttatta cgcatcaaat aaaacggcag taaatgaaaa agactgtgtg actttggatg
     8041 ttattgggga ttccaaatat gatacaaacc tcaccattat agcgaaaagg actataagcg
     8101 gaactgtatc tttagaaaag ggtgagactg ctccccagga aggagtgtct gttcgtatca
     8161 gtgcccagga aggtgatgag caaactgtcg tcatacctta cggtaaaaac tctgcatcat
     8221 acgccctgaa ggtggtgccg aaggatttag gatatggcta caaggtaaaa tacgaaaccc
     8281 tcaagaatta tggctatata cgatacggct attattccga agacggttgt gtgagaaatg
     8341 aggcaaaggc tgatattgtt gatgtcagtg acggtgacag ggaaaatata gacattgaga
     8401 tagtaaggcc gcgtaccata gaaggaaaac taaggcttcc tgaaggtgtt gcggcaagca
     8461 aagacatgac ggtaagtata attgcttcca acagtttaga cagtgccgaa gtttcggtct
     8521 acgtaccgaa aggttccagc gaggctttat attcgcttaa cgttcctccc aatgacaaga
     8581 atgacgcata taaagtcaga tatgagaatt ggtcggacaa cagttttgca gatataggtt
     8641 attacagcaa gtcggaaact gtaagaaatg ctggcttggc agagagtgtc aatgtaagga
     8701 atcaaaatgc aagcggtatt gtacttacat taatagcaaa aagaaacgta tctggaaagg
     8761 tttctcttcc caacggttct gctcctgcgg gtgggcttac ggtaaccgtt tatcttgaaa
     8821 atgatacgga taaggttgta tcttacgcta ctatactgca aaatcaaagc tctgctgact
     8881 actcggtaaa tgtaccggtg ggcaaaaatt ataaagtcgg ctatgaaata tccacaaaga
     8941 ataatttggt tccatggggt tattatggtg cctatggtac ggtttatatg cccgatagtg
     9001 catatctttt ggatgtaacc gatgatattg acggaattga tatgacactt ttagagaaaa
     9061 agtccatcag cggtacggta tctctacctg aagggaaagc tccaaaggga ggagtcaagg
     9121 tagaaatata cgcggaggac gccggagaca catgggttac aattcctgaa ggagaatcct
     9181 ctgcccagta ttcaatgaag gttgtgccaa gtgttgaagg aaaaggctat aaggttagat
     9241 atgtggtttc acaggatcac gatcttgtga attacggttt ctacagcaag gagggcacgg
     9301 taagaaacag caagctggcg gacccggtat atgccaacaa taaagatgta acggacataa
     9361 atattgaaat tatgaagcca agaaagatta gcggaaaggt atccttaccg gaaggtgttg
     9421 caccggaagg aggcatttca ttgaacatag ccgtgaccaa tgacgtagat ggaaattcgc
     9481 agataataat cataccggag ggtgaaagtt ttgcagagta ttccataagt cttcctccga
     9541 acgatccggg ttatgagtac aaagtgaggt atgagaactg gtcaaacaag gtatttacaa
     9601 cgtatggata tttcaacagc aaaggcacta caaataatct atcctatgca gaaccggtag
     9661 atgtcaataa aactgatgca aataatgtag atatggtttt attgagaaaa tcaaccatta
     9721 gcggaaactt cgaggttccg gaggggtgtg tccttcctga agaaggtctt catgtaagaa
     9781 tctatgtgtc aaacgatgtt gaaacctatt catcgaatat aacagtacct tacggcacaa
     9841 catcggttcc atactctgtt cacgttgagg aaggatcggg ttacaggctt ttctatgcat
     9901 tagacagtaa tgacagattt atggaatatg gttattatgg tgaagacggt gtatatacgg
     9961 ataaaaagag agcaaaagta tttactgtaa ccgatgaaaa cataagcgga tgcaagctga
    10021 gacttctcga aaagagaata atatcaggaa agtttacgtt gccttatgat cttgacgaag
    10081 tgggctcaat tgaagttagc atcagtgctt caaacggatt agacagaggt gaaaccaaaa
    10141 tacgtgttcc ttatggagaa acagaggttg attatacact gaatttgccg gcgggcagcg
    10201 gatatatact gcaatacgac attattacca taaaaggttt tgcatcttcc ggatactaca
    10261 gtgagggtgg tatggtaaga agcaaggata aagcggatac tctggatttg gcggaaaatg
    10321 acctgacagg tatatacatg tctttgatag aagatatgac gataaacggc ataatcaaac
    10381 ttcctcaagg agtagctcct gagggtggta ttgaagtaac ggttaccgca acggatgagt
    10441 cgggtaatac atcatcatgc ataagaacaa taaaagaagg tgaaaattct gttgagtatt
    10501 acctgaatgt tcctccaagc aattataatt caggttataa ggtaagatac agcgtaacct
    10561 ccgatgacta tgcaaccctt ggatattaca gcgaaagcgg tacgatagta ctgccggatt
    10621 tggcaacacc tgtggatgtt agcagtgaga atgcagaaga cataaatctg atgttgattg
    10681 aaaagaaggt tataagcggg gttgtaagta taccgggcac agtcgctaaa gagggcggat
    10741 tagctataac cataagggct tccagccaat tgttcagtgt ggatgacagg atggaggtag
    10801 taatacccga aggagaaagc aaggcttact attccctgaa ggtatctcca aatgttgaag
    10861 gtgcagatta tgtaatattc tatgaggttg atgatgacgc atatgtcggc actggatatt
    10921 ataatacagc cggaacggtg gttgatatca aaatggcaac ccctgttgac gtaagcaacg
    10981 gtaattatag caatgcggac atttcactgg taagaagcag acaaatttca ggaagtatag
    11041 cacttcccga tggagaaacc gcacctaagg gtggggtatc gataacggtg tttgcggaga
    11101 aaacagatta tacaggctat agagccagca agactatatt gatacctgaa agtaaaaact
    11161 gggcagaata taccctgatt gttcctgaaa gcacatccaa ggttgtggga cttgatattg
    11221 aggcatcaac cgataatgga accggtgata cggcagatga ttatactctc agcacaaagg
    11281 tgtatattcc atcggcaagt aacaaccgta actctgatta catggttggc tattcatatg
    11341 ttgccggtga aaagtatttt aagagcggat tttattccaa agccggtaca gttcctgcaa
    11401 aaggtatggc agatatattg gatgcagccg gaaaagacat tgagaatatc gactttacac
    11461 tactgaaaaa agacacgaca ataaaaggca ctgttaagct tccggaagga aagactgcac
    11521 caaaggatgg tatcacggta agtataacag ctgaaaacga tgcattggat ttcgtaagtg
    11581 aaaagacagt tgttatcagt tccggaaatt cgactgttgg atatgagatt gaggttcctt
    11641 cgattgacga ttatcatata aagtacacaa taaagtcaac aggcggatat gttaccagtg
    11701 gttattattc cgaagaagga actgtaggca caaaggaacg ctcaacatca attaactgtt
    11761 cctccggtag tgtaacggga attaatttga atttgatacc aggaatagag ataagaggaa
    11821 agatattgtt gccgtcaggc cacaaggtta atataaaaga cttctggatg tgggttgtgg
    11881 catcaaacga aaattatgaa tccacggctt atattaccat atctgaagga agttctgttg
    11941 ctaactatag tgtgtatgtt ccagaaggat ccgactatgt tgtaagctat tctctgatgc
    12001 cactgtttgg tgagtatata gaaaagggat attataattc cagtatgacc acggcaaaca
    12061 aaaactgtgc aacaaagctc aatgtaacaa aaaatatcag cggtatagaa atgacactga
    12121 tacctgttga ccgtattgtt tccggaacag tatcactgcc tgacggtacg gctgcagtac
    12181 cctactcaat acgtgttcct tcaaacaacc ggggcaacgg ttaccgggtg ggatattcag
    12241 tgacatcagg aaatactgat ggattatata atgaaaatgg gtatttcagc ccctttggaa
    12301 ctaccacaaa cattaatgaa gcttcaacca tcgatgttag cataagaaac agcagcgata
    12361 gggatatgac actgataact gctgatatga agcctgttga agcaataatg ctggataagt
    12421 atcaagtgac attgcaaagt ggtgga
//
LOCUS       DAOOJC010000010        12389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_4707_length_12389_cov_3.903519, whole genome shotgun sequence.
ACCESSION   DAOOJC010000010 DAOOJC010000000
VERSION     DAOOJC010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 12389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12389
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4707_length_12389_cov_3.903519"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..1499)
                     /locus_tag="PLA01_00560"
     CDS             complement(168..1499)
                     /locus_tag="PLA01_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HPC99822.1"
                     /translation="MQDFLSFVICSLITVIGNAFLTALTAEIIYNTRFRVRLITVYSV
                     TYIFVLGMIVAFSDYVLIRFSPNLAMTFRTFFCAVAAIILLKCVFKLKLANCITCEFF
                     FLLFLAIGNVVMSLIFKTLGIDTSNIKMSLFYIIVGIVIIDSVVLIILAIIKSFRMFS
                     CFNTEIKSKAYKTNILYICLVFAILILNFNYYEKYAKLTDILTLLISVLLLIVFLLLS
                     LFNTNTFFKLETKSQELEYQLFYNKALDAIIKDLRRFKHNYNNILSVFGGYIKTKKWD
                     EVERYYTEICGDISKVSFLDNLSMANIKNAGILWLIMSKYENARELKVDFRVVTEGVI
                     GEIGMKMSEFCEIMGILLDNAIEAASASERKVVELFIRKIDGSISFVIENAVLEKVNV
                     AKLYEEGYSTKGEGRGLGLYILKRIVSKYENVILNTQAEETRFKQELIINP"
     gene            complement(1762..3150)
                     /locus_tag="PLA01_00565"
     CDS             complement(1762..3150)
                     /locus_tag="PLA01_00565"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPC99823.1"
                     /translation="MEVEKTMEKIVALSKNRGFVYPGSDIYGGLANAWDYGPLGVELK
                     NNVKRAWWRKFIQENPYNVGVDCAILMNPQVWVASGHVGGFSDPLIDCKDCKTRHRAD
                     KMIEDWNNENGQNVRVDGWTKEQLVEYIKEKGVSCPNCGSKNFTDIRQFNLMFKTFQG
                     VTEDSKAEIYLRPETAQGIFVNFKNVQRTTRRKIPFGIGQIGKSFRNEITPGNFIFRI
                     REFEQMELEFFCKPGEDLEWFDYWKNYCYNWLLSLNINKDNLKMRDHSKEELSHYSNA
                     TTDIEYKFPFGWGELWGIADRTDFDLKQHAEHSKEDLSYFDPNTNEKYVPYCIEPSLG
                     ADRVTLAFLCEAYDEEEVAEGDIRVVLHFHPALAPVKITVLPLSKKLGDDAFKVYEML
                     TKKYTCEYDETGSIGKRYRRQDEIGTPYCITFDFDSLNDQSVTIRDRDSMQQVRVKID
                     ELLSYFEGKFEF"
     gene            complement(3190..4404)
                     /gene="coaBC"
                     /locus_tag="PLA01_00570"
     CDS             complement(3190..4404)
                     /gene="coaBC"
                     /locus_tag="PLA01_00570"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPC99824.1"
                     /translation="MLKGKTVVVGVCGGIAAYKSVEIVSRLKKLNADVHVIMTENAAR
                     FVEPLTFRSLSHNPVTIDMFDEPKAWDIQHISLAEKADLIVVAPATANIIGKVAAGIA
                     DDMLSTTIMAADSPVLFVPAMNFRMYENPIVQQNINKLAGLGYIFMEPESGLMACGTS
                     GRGRLPSPETIVSFVKGILTKNRDYSDIRVLVTAGPTREAIDPVRFITNPSSGKMGYS
                     IAAAACERGAQVRLISGPVNIVPPCGVETVYVTSAMDMYSEVMKAYESFDVLVMVAAV
                     ADYRCAQVAQKKIKKSDDNLTVELVRNPDIAAELGKVKGDKILVGFSAETDNLVKNAS
                     GKLASKNMDMIAANDVTVEGAGFGTDTNVVKLIKRDGSILDLPVMSKKDVAHRILDEI
                     LVVKNGNSVDKT"
     gene            complement(4550..4786)
                     /gene="rpoZ"
                     /locus_tag="PLA01_00575"
     CDS             complement(4550..4786)
                     /gene="rpoZ"
                     /locus_tag="PLA01_00575"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPC99825.1"
                     /translation="MKDQNENKSTMIEPGISSLLTKVDSRYTLVVATAKRARQLTEGA
                     HKLTDCNSDKLVTIAINEINEEKITYIRTKSGIK"
     gene            complement(4874..5476)
                     /gene="gmk"
                     /locus_tag="PLA01_00580"
     CDS             complement(4874..5476)
                     /gene="gmk"
                     /locus_tag="PLA01_00580"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPC99826.1"
                     /translation="MFREGLLVIVSGPSGTGKGTVLKHLKDSDDSIRFSVSATTRTIR
                     QGEMEGLNYFFKTREEFESMIKNSQLLEWVEYCGNYYGTPKEYIESTIKEGYDCLLEI
                     EVEGAANVKKTYPDCVSVFILPPSFEELKRRIQNRATEEPENVIRRLERAKEEMAYIK
                     NYDYVIVNDKIEDAVESLRSILKAEKLKFNRNCKIIEQLK"
     gene            complement(5491..5772)
                     /locus_tag="PLA01_00585"
     CDS             complement(5491..5772)
                     /locus_tag="PLA01_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HPC99827.1"
                     /translation="MLMKLINIGFGNIVSANRLVAIVSPESAPIKRIIQEARDRGMLV
                     DATYGRRTRAVIITDSDHIILSAVQPETVAHRLNSKEADVVEVDEDIEE"
     gene            complement(5783..6661)
                     /locus_tag="PLA01_00590"
     CDS             complement(5783..6661)
                     /locus_tag="PLA01_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPC99828.1"
                     /translation="MIKSMTGFGRGKIEDDGKELMVEIKTVNHRYCDIYIKMPRQISF
                     MEDKVREIIRKNISRGKVDVYISYDDYGEDSKDIIIDKALAKAYSKSVEHLRDEFGLK
                     DDISVSLIARFPDVLRVEKPEEDEEKIWSLLSEALKDALDALINMRQIEGEGLMLDLL
                     ERTTLIEKNIKEISIRAPEVVKEYKCKLENRIKELLDQQIVDENRIATEVAIFADRCN
                     IDEELVRLGSHIKQLRETLRLSQPVGRKLDFLLQELNREINTIGSKANDLIITNQVVE
                     IKSELEKIREQIQNIE"
     gene            complement(6880..7266)
                     /locus_tag="PLA01_00595"
     CDS             complement(6880..7266)
                     /locus_tag="PLA01_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC99829.1"
                     /translation="MKNLRFVVVDDAVFMRTLLKKMIEEERTYKVVGEGSNGYEAIEQ
                     VKIYKPDIVTLDITMPEMDGIAAIKGILECSPDTKIIVVSAMGQQSMVIEAIKLGAKD
                     FVVKPFDKTRVMQAIKNVTGDSDTLF"
     gene            complement(7263..8009)
                     /locus_tag="PLA01_00600"
     CDS             complement(7263..8009)
                     /locus_tag="PLA01_00600"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HPC99830.1"
                     /translation="MSRFFVNSTNISGDSIIISGEDVNHIKRVLRLGSGDNITVSDGK
                     GVDYRVEIVNLHESAIETRIIETVKNITEPPVEIILYQGLPKSDKMDYIIQKGVELGL
                     KGIVPVITERTVVKLTDKKDEQKKCERWNRISMEAAKQSNRGIIPPVELPIDFKKAVN
                     AAKDFDISLIPYEKERGNGLKDVILNRTDAKKVSIFIGPEGGFTEKEIETAVENGVYP
                     VTLGPRILRTETAGIAVLSILMYELGDVGK"
     gene            complement(7991..8935)
                     /gene="prmA"
                     /locus_tag="PLA01_00605"
     CDS             complement(7991..8935)
                     /gene="prmA"
                     /locus_tag="PLA01_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPC99831.1"
                     /translation="MKWNEIKIKTSEEAYDSVSYMLTSIGAGGVAIEDPNDIRKEILK
                     PNSLDYADEEFLNSLGEDVVIKAYFLGETNVSEIIALIREKLTYINNFLNVGEGYIGY
                     CEVDDEDWSTSWKKYYKPFELTDGLVVKPTWEKYDNKDDETVIELDPGMAFGTGTHET
                     TKMCAAFLHKYLKSGYSVIDVGCGSGILSIISCKLGASRVTAVDIDEVAVKVTKENCA
                     INRVDHSVTVFKGVVTDIKNEKADIVIANIIADVIIDLSSSIPCYLKEGGIFITSGII
                     KERKDEVLEKYLSEGFKCEETIEMGEWVAMALRCLGSL"
     gene            complement(8951..10102)
                     /gene="dnaJ"
                     /locus_tag="PLA01_00610"
     CDS             complement(8951..10102)
                     /gene="dnaJ"
                     /locus_tag="PLA01_00610"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPC99832.1"
                     /translation="MAGKRDYYEVLGVEKGASDAEIKKAYRKLAKQYHPDMNPGDKAA
                     EAKFKEVNEAYEVLSDSQKRAQYDQFGHAATDGSGFGGFGGFGGFGDFDLGGFGDIFE
                     TFFGGGGFSSRTSRSTRGPQRGSDLKQTLEISFEEAAFGVNKEIIVNRMDVCTTCSGS
                     GSKPGHQPATCHQCNGTGQVQYKQRTPFGQFVNIKTCDVCHGEGKIITQPCESCGGKG
                     RVRKHIKLEISIPAGIDDGQILPVRGQGEPGVRGGPSGDLFLSIRVKPHPFFKRNGFD
                     VYCEVPITFVQAALGAEIEVPTLDGKEKLVIPEGTQTGTVFRFKGKGITVLRETRRGT
                     QHVKVNVEVPKKLSEKQKEVLRQFAELTGDEVHEQRKSFFDKMKDALGM"
     gene            complement(10453..12294)
                     /gene="dnaK"
                     /locus_tag="PLA01_00615"
     CDS             complement(10453..12294)
                     /gene="dnaK"
                     /locus_tag="PLA01_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPC99833.1"
                     /translation="MAKVIGIDLGTTNSCVAVMEGGEPVVIPNSEGGRTTASVVAFSK
                     TNERLVGQVAKRQAITNPERTVISIKRDMGTDKRVKIDDKNYTPQEISAMILQKIKAD
                     AESYLGQPVTQAVITVPAYFSDSQRQATKDAGKIAGLEVLRIINEPTAAALAYGLDKE
                     TDQKILVFDLGGGTFDVSLLEIGDGVFEVLATSGNNRLGGDDFDDRIINYLVDTFKRE
                     NGIDLRNDKMALQRLKEAAEKAKIELSGVTTTNINLPFITADATGPKHLDITLTRAKF
                     DEITSDLVEMTMEPTRKAMKDADLTPDKIDKILLVGGSTRIPAVQEAVKKYFGKEPFK
                     GINPDECVAIGAAIQAGVLTGEVKDLLLLDVTPLSLGIETLGGVFTKLIERNTTIPTK
                     KSQVFSTAADGQTAVTVRVFQGERAMAADNKLLGEFNLDGIPPAPKGVPQIEVTFDID
                     ANGIVHVSAKDLGTGKEQNITITASTNLSDSDIEKAVNEAKKFEEEDKKRKESVEIRN
                     NADSMVFQSEKTLRDLGDKLSSEDKSKVEAEIEKVKEALKGNDTDAIKSATEELQKAF
                     YDISSKIYQQNQANPNTSDGAGAQGGANGGQENVYDADYKVVDEEDK"
BASE COUNT         3435 a         3028 c         1858 g         4068 t
ORIGIN      
        1 aaatacccgt ggctagactt tcactagcta gattagtgcc atgcctggca cacactctaa
       61 aaaaggctcc ggatctcccg gagtcttttt taaccgggaa agtacattaa ctttttatat
      121 attcactatc gaagtgcaag gtttataaaa caggaataaa cattaaatta aggatttata
      181 attaattcct gcttaaatct tgtttcttct gcctgggtat tcagtattac attttcatat
      241 ttagaaacaa tccttttcaa aatataaagc cctaatcccc tgccttctcc tttagtcgaa
      301 taaccctctt cgtagagctt cgcaacattc accttttcca aaactgcatt ttctataaca
      361 aagctgatac ttccgtctat ttttctgata aacagttcca ctaccttcct ttcgctggca
      421 ctggctgcct ctattgcgtt atccaaaaga attcccataa tctcgcaaaa ttctgacatt
      481 ttcataccta tttcaccaat aacaccctct gttaccactc taaaatcaac cttcaactct
      541 ctggcattct catatttact catgatgagc cataaaatac ccgcattctt gatatttgcc
      601 attgacaggt tatccaaaaa actaacttta gagatgtctc cacatatttc agtatagtat
      661 ctttctacct catcccactt ctttgtctta atgtagcctc caaacacgga aagaatattg
      721 ttatagttat gcttaaacct tctaagatcc tttataatag catccaacgc cttgttgtag
      781 aaaagttggt attcaagttc ctggcttttt gtttcaagct tgaagaaagt gtttgtatta
      841 aacaatgata gcaatagaaa aacaataagc aggagtacac ttattaaaag tgttaaaata
      901 tccgtcagct tggcgtactt ttcataataa ttgaaattta aaattaaaat tgcaaataca
      961 aggcatatat ataatatatt ggttttgtac gctttcgact ttatctccgt attaaaacaa
     1021 gaaaacattc tgaaggattt tattatggca agaattatta atactacaga atctattata
     1081 actattccaa caatgatgta gaataatgac attttgatat tgctggtatc aattcctaat
     1141 gttttaaata ttaatgacat aacaacattt ccgattgcaa gaaataataa aaagaagaat
     1201 tcacaggtaa tacaattggc aagctttagc ttaaaaacgc atttaagcag aattatggca
     1261 gctacagcac aaaaaaaagt tctaaacgtc atagccagat ttggtgaaaa cctaatgagg
     1321 acataatctg aaaaagccac aatcatccct aaaacaaata tgtatgtaac agaatatact
     1381 gttatcagcc tgacccgaaa tctggtattg taaataattt ctgcggtaag tgccgttaga
     1441 aaagcattac caattactgt tatcaggctg caaataacaa agcttaaaaa atcctgcaaa
     1501 ataccccctc cttagatttt ttgtaatatc ccgctatatt tttatgtata cataaaacac
     1561 acatatcttc aaatacaata atacacgaaa atccgcttat tgacaaagaa aaaataaaag
     1621 ctcacgagca ccgggcccat tcgctttttg tcatttaact tcgtgaaatc ccactaagtt
     1681 aaaagggcat taccgaaaaa tagtaatgcc cgcaaatact cattttacat tttacaaaaa
     1741 taacgttttt atacgataat gctaaaactc aaactttcct tcgaaataag aaagcaattc
     1801 gtcaattttt actcttacct gttgcatgga gtcacggtct ctgattgtaa cactttggtc
     1861 attcagtgaa tcaaaatcaa aggttataca gtatggagta cctatctcat cctgtcttct
     1921 gtatcttttg cctatacttc cggtttcatc atattcacag gtatacttct ttgtaagcat
     1981 ttcgtaaacc ttgaacgcat cgtccccaag ctttttagac agaggaagaa cagttatctt
     2041 taccggtgct aaagcaggat ggaaatgcag tactaccctt atatcccctt ctgctacttc
     2101 ttcctcatca tatgcctcgc acaggaaagc aagagttacc ctgtcggcac caagagatgg
     2161 ctctatacaa taaggaacgt acttttcatt tgtattggga tcgaaatatg ataagtcctc
     2221 cttcgaatgc tcagcatgct gttttaaatc aaaatccgtt ctgtcggcaa ttccccataa
     2281 ttctccccaa ccgaagggaa acttatattc aatatccgtt gtggcattgc tgtaatggga
     2341 taattcttcc ttgctgtggt cacgcatttt aagattgtct ttattaatgt taaggctcaa
     2401 aagccaatta tagcaataat tcttccaata atcgaaccat tccaggtctt cgccgggttt
     2461 acagaagaat tccagttcca tctgctcaaa ttcacggatc cggaaaataa aattacccgg
     2521 tgtaatttca tttctgaagg actttcctat ctgcccaata ccgaagggta tcttccttct
     2581 ggttgttctt tgaacattct taaagtttac aaatatgccc tgagccgttt cgggtcttaa
     2641 gtaaatctca gccttagaat cctcggtaac cccctgaaag gtcttgaaca taagattgaa
     2701 ttgccggatg tctgtaaagt tcttagaacc gcagttcgga caactaactc ccttttcctt
     2761 aatgtattca accagctgct cctttgtcca gccgtcaacc ctgacattct gcccattctc
     2821 gttattccag tcttctatca tcttgtctgc cctatgacgc gttttgcagt ccttacagtc
     2881 aataagagga tcactgaatc ctcccacatg ccctgaagcc acccatacct ggggattcat
     2941 caaaatggca cagtccacgc caacattata tggattttcc tgaataaact tcctccacca
     3001 tgcccttttt acattgttct tcagctcaac acccagcgga ccgtaatccc atgcattagc
     3061 caaacctcca tatatatcgg atcccggata tacaaacccc cggttttttg acagagctac
     3121 tattttttcc attgtcttct caacttccat tatgttgcct ccttaaaccc atacttattt
     3181 attaatattt caagttttat caacactgtt tccgttctta acaaccagaa tttcgtccaa
     3241 tattctgtgg gcaacgtctt ttttactcat aacgggcaaa tccaaaatac tgccgtctct
     3301 ttttatgagc ttcaccacat tggtatccgt tccaaaccct gcaccctcca cagtcacatc
     3361 attggctgca atcatgtcca tgttctttga tgcaagcttt cccgatgcgt tttttacaag
     3421 attatcggtt tctgcactaa aacccacaag aattttatca ccctttactt tgccaagctc
     3481 tgcggctata tcagggtttc taaccaattc tacggttaaa ttgtcatcgg actttttaat
     3541 tttcttctgt gcaacctgtg cacacctgta atctgctacc gcagccacca ttaccaaaac
     3601 atcaaagctt tcgtatgcct tcattacctc gctatacata tccattgcac tggtaacata
     3661 aaccgtttcg actccgcagg gcgggacaat attaaccggt cccgatatca atctgacctg
     3721 tgcacctctc tcgcaggccg cagccgcaat tgaataaccc attttccctg atgaagggtt
     3781 ggtaataaat ctaacgggat caattgcctc cctggtcgga ccagcagtca caagaaccct
     3841 tatatctgaa taatctctat ttttcgttaa tatcccttta acaaagctaa caattgtttc
     3901 gggtgaaggc agccttcccc tgccgctggt tccacatgcc attaacccgg attcaggttc
     3961 cataaaaata taaccaagcc cagcaagctt attaatattc tgttgaacaa taggattctc
     4021 atacatcctg aagttcatgg caggtacaaa aagaaccgga gaatcagctg ccataatagt
     4081 ggtagaaagc atatcatctg ctattccggc agcaaccttt ccgataatat tggctgttgc
     4141 aggagcaaca acaatgagat cagccttctc agctaaagat atatgctgta tatcccatgc
     4201 cttaggctca tcaaacatgt caattgtaac gggattatgg gaaagagatc tgaaggtaag
     4261 aggctcaaca aacctagcag cattctcagt cattatgaca tgaacatcag cattaagctt
     4321 ttttaacctg cttacaatct ccactgactt ataagcggct attccgccgc aaacaccgac
     4381 tactacagtt ttccccttta acaattttcc cgcctccccg aaaagtcaaa caccaatctt
     4441 aaatttattg attaaaaaaa taagatcagg accatgattg cggcagctgt ctattgaacc
     4501 tcagacatca atacttcctg attgatcttt aatgtaaaaa aaaatcaact tatttaattc
     4561 cgctttttgt ccttatgtat gttatttttt cctcgttaat ttcatttatt gcaatggtga
     4621 caagcttgtc agaattacaa tccgtaagct tatgagcccc ctccgtaagc tgtcttgccc
     4681 tcttcgcagt agctactacc agagtatatc tgctgtcaac cttagttagc agagagctta
     4741 taccaggttc aatcatagtt gatttgtttt cattttgatc cttcataact tagtgcaacc
     4801 tccctcagtt ttaagattca aaggattttt tttaaaccat tttgcattat ttcatcggca
     4861 aataacatac cttttattta agctgctcaa ttattttgca gtttcgatta aatttcagtt
     4921 tttccgcctt caatatagag cgtaaacttt ctacagcatc ttctattttg tcgttgacta
     4981 tgacatagtc atagtttttt atatatgcca tttcctcttt tgccctctcc aggcgtctta
     5041 tgacattttc aggttcctca gttgctctat tctgaatcct tcttttcaac tcttcaaaag
     5101 aaggggggag tataaatact gatacacaat cgggatatgt tttcttgaca tttgctgctc
     5161 cctccacctc aatttcgagg agacagtcat atccctcttt gatagtactt tctatatatt
     5221 ctttcggagt accgtaataa ttgccgcaat attccaccca ttcaagaagc tgactgtttt
     5281 ttatcattga ctcaaactct tcccgggttt tgaaaaaata attcagccct tccatttcgc
     5341 cctgtctaat cgtccttgtc gtagctgaca ccgaaaatct aatactgtcg tcactgtctt
     5401 ttagatgttt caaaaccgtg cccttgccag taccggacgg accggataca ataaccagta
     5461 gtccctcacg gaacatattt aaagttcccc ctattcttca atatcctcat ccacttctac
     5521 cacatcggct tccttcgaat tcagtctatg tgccactgtt tctggctgaa cagccgatag
     5581 aataatgtgg tcgctgtctg taataattac agcccttgtt ctcctgccat aagttgcatc
     5641 aacaagcatt cccctgtcgc gggcttcctg tattattctt tttataggag cggattccgg
     5701 gcttacaatt gctacaagtc tgtttgccga cacaatattt ccaaatccaa tattaatcaa
     5761 cttcattaaa atacctccta aatcattcaa tattttgtat ctgctccctg attttttcta
     5821 attcactttt tatttcaaca acttgattag ttataatcaa atcgttagcc ttagagccta
     5881 tggtattaat ttccctgttc aattcctgaa ggagaaaatc aagttttctt cccaccggct
     5941 gacttaatct caaggtttcc ctaagctgct ttatgtggct tcccaaccta accagttctt
     6001 cgtcaatatt gcacctgtct gcaaaaatag caacctctgt agctatacgg ttttcgtcta
     6061 caatctgctg atccaataat tcctttattc tgttctcaag cttacattta tattctttga
     6121 caacttcagg tgctcttatg ctaatttctt taatattctt ttcgataagg gttgttcttt
     6181 caagcaagtc aagcatcaga ccctctccct caatctgcct catattgatt aaagcatcaa
     6241 gggcatcttt caatgcttca ctcaaaagac tccatatttt ttcttcatcc tcttcaggtt
     6301 tttccaccct caaaacatca ggaaacctgg caataaggga caccgatata tcatccttca
     6361 aaccgaattc atccctcaaa tgctcaacac ttttggagta agccttagcc aaagccttat
     6421 caatgataat atcctttgaa tcttcgccat aatcatcata acttatgtaa acatcgactt
     6481 ttcctcttga gatattcttc cttattattt ctcttacctt atcctccata aaggatatct
     6541 gtcttggcat ctttatataa atatcgcaat acctatggtt taccgttttt atttcgacca
     6601 ttaactcctt gccgtcatct tcaattttgc cccttccaaa tcccgtcata ctcttaatca
     6661 taaagccgtc catccccttc atgggttttc tttattccaa tacgtattat aacataattt
     6721 tagacaatta aaaggctttt gctttaattt ttatatgttt tttcttaata tctcctattt
     6781 cctttatttt agaaaacatt ccggctgaca ccagcaaaaa aaatactgta ttcgttaaaa
     6841 caagataccc aaacccaaat gtattttcta ttgtaatact taaaatagtg tatcagagtc
     6901 gccggtaaca ttttttattg cctgcattac cctggtttta tcaaagggct ttacaacaaa
     6961 atcctttgcc ccaagcttta tagcttctat taccatggat tgctggccca ttgcggatac
     7021 cacgataatc tttgtgtcgg ggctgcattc aagaattccc tttattgcag caatgccatc
     7081 catttcgggc attgttatat caagggttac tatatcaggc ttatatattt taacctgttc
     7141 gatagcctca taaccgtttg aaccctcacc aaccacttta tatgttcttt cttcctcgat
     7201 catcttttta agcaatgtac gcataaaaac cgcatcatca acgacaacaa atctgagatt
     7261 tttcatttgc caacatctcc taattcatac atcagtattg ataaaacagc aattccggca
     7321 gtttctgtcc tcaatatcct gggacctaat gttaccggat atactccgtt ttcaacagct
     7381 gtctctatct ctttttcagt aaaaccacct tcaggcccga taaaaatcga aaccttctta
     7441 gcatcggtcc tgtttaaaat aacatccttt agtccattgc ctctctcctt ttcataagga
     7501 atcagagaaa tatcaaaatc ctttgcagca tttacagcct ttttaaaatc tataggcaat
     7561 tccaccggag gaatgatccc cctgttggac tgttttgcag cttccataga aattctgttc
     7621 catctctcgc atttcttttg ctcgtctttt ttgtcggtca gctttacaac agttctttct
     7681 gtaataacag ggactatccc ttttagtcca agttctacac ctttttgtat aatataatcc
     7741 attttatcgg attttggcaa cccctgatat agaataattt caacaggagg ctctgttatg
     7801 ttcttaaccg tttcgattat tcgggtctca attgctgatt catgcagatt cacaatttca
     7861 accctgtagt caacaccttt tccgtcggaa accgttatgt tgtcgccact ccccagtctc
     7921 aatacccttt ttatatgatt gacatcttcg ccggaaataa taatcgaatc accggatata
     7981 tttgtgctgt ttacaaagaa cctagacatc ttaaagccat cgccacccat tctcccatct
     8041 cgattgtttc ctcgcacttg aatccttcgc tcaggtattt ttctaatacc tcgtcttttc
     8101 tttccttaat gatgcctgaa gttataaaaa tcccgccttc cttcaaatag cacggtatgc
     8161 ttgaagataa atcaattata acatcggcaa taatattggc aataactatg tctgcttttt
     8221 cattttttat atccgtcaca acacctttaa aaacagttac tgaatggtcc accctgttaa
     8281 tcgcacaatt ttcttttgtg acttttactg caacctcatc aatatcaacg gcagtaaccc
     8341 ttgatgctcc aagcttgcat gatataattg ataaaatgcc cgagccgcac ccgacgtcaa
     8401 taacactata tccgcttttc agatacttat gaagaaaagc ggcacacatt ttcgtagttt
     8461 catgagtccc ggtgccgaat gccattccgg gatccaattc aatcactgtt tcatcgtctt
     8521 tattatcgta tttttcccat gtcggcttaa caactaaacc gtccgtcagc tcaaacggct
     8581 tgtaatactt tttccacgat gtactccaat cttcatcatc aacctcgcaa taaccgatat
     8641 agccttcacc cacgtttaaa aaattattaa tataagtcag cttttccctg atcagggcaa
     8701 taatttcaga aacattggtt tcgcccaaaa aataagcttt tataaccaca tcttcaccca
     8761 aagaatttaa aaactcttca tcggcgtagt ccagtgaatt gggctttaga atctcttttc
     8821 taatgtcatt gggatcctct atggcaactc cccctgctcc tatggaagta agcatgtagg
     8881 ataccgaatc atatgcttcc tcacttgtct ttatcttaat ctcattccac ttcattattt
     8941 atcctccatt ttacatgccc agagcatcct tcatcttgtc gaaaaagctc ttcctctgct
     9001 catgcacctc gtcgcctgtc agttcagcaa attgtcttaa gacttccttc tgtttttcac
     9061 tcagtttttt gggaacctca acattaactt taacatgctg tgttcccctt ctggtttctc
     9121 tcagtacagt tattcccttg cccttaaatc taaacaccgt accggtctga gttccttcag
     9181 gaataaccag cttttccttg ccgtcaaggg ttggaacctc tatctcagcc cccagtgcag
     9241 cctgcacaaa agttatcgga acttcacaat aaacatcaaa cccgttcctc ttaaaaaacg
     9301 gatggggttt aacccttatg gataaaaaca gatctccgga aggtcctcct ctaacaccgg
     9361 gttctccttg acccctaacc ggtaaaatct gaccgtcatc tataccggcc ggtatactga
     9421 tctcaagttt tatatgcttt cttacccttc ctttgccacc gcaactctca cagggctgcg
     9481 taattatctt tccctcccca tggcacacgt cacaggtctt tatatttaca aattgtccaa
     9541 aaggtgtccg ctgcttatac tgtacctgac ccgttccatt acactgatgg catgtagcag
     9601 gctgatgtcc cggctttgaa ccggagccac tgcatgtagt acaaacatcc atcctattaa
     9661 ctattatctc cttgttgacg ccaaacgcag cctcttcaaa gcttatttca agtgtctgct
     9721 tcaaatcgga acctctttgg ggacctcttg tgctgcggga ggttctgctg ctaaatccac
     9781 ctccaccgaa aaatgtctcg aatatatcgc caaaacctcc aagatcaaag tcgccaaaac
     9841 ctccaaagcc gccaaaacct ccaaaaccgc tcccatctgt tgcagcatgc ccgaattggt
     9901 catattgagc ccttttctgc gaatcactta aaacctcata ggcttcattt acttctttaa
     9961 actttgcttc ggcagcctta tctccgggat tcatatcggg atgatattgc ttggccaact
    10021 tcctgtaagc cttctttatt tcagcatcgg atgcaccttt ttcaacgcca aggacttcat
    10081 aataatccct tttgccagcc atccaaccaa ctcctccata taaattctat attaatcgca
    10141 ccattcttta aataaatata attacatata tttgcctatt tatataaatt tgcccaatca
    10201 ttctttttct tctttcagat tataccacat aggggcattg tactgcaacg aaagttattg
    10261 caaactacag gcataatatg tagagaagct tcatagttac aaaagagcca aagccatctt
    10321 catgactttg gcctttatac cgttaataac gtatcggtca aaagtgattt tcacggtttt
    10381 atataatttg ctcgaatccc tgcgattcaa cgaggcaggg catactcacc gcctgaaaac
    10441 ccactccaat tactatttgt cttcttcatc aacgactttg taatcggcat catagacatt
    10501 ctcctgtcca ccattggcac caccctgtgc accggcacca tcactggtgt ttggattcgc
    10561 ctgattctgt tggtatatct tcgaagaaat atcatagaat gccttctgca gttcttctgt
    10621 agcactctta attgcgtctg tgtcatttcc cttcaaagct tctttaacct tttcaatttc
    10681 agcttctact ttcgatttat cctcactgct cagcttatcc ccgagatctc ttaacgtctt
    10741 ttctgattgg aaaaccatgg agtcagcatt atttctgatt tccacgctct ctttcctctt
    10801 tttatcctct tcttcaaatt tcttagcttc atttacagcc ttttcaatat cactatccga
    10861 caaattggta gatgccgtta ttgttatgtt ctgttctttt cctgtaccca aatcctttgc
    10921 cgaaacatgt acaataccgt tggcatcaat gtcgaatgtt acttcaattt gaggaactcc
    10981 cttcggtgcc ggaggaatac cgtcaagatt gaactctcct aaaagcttgt tatcggcagc
    11041 catggctctt tcgccctgga ataccctgac agttacggcc gtctgaccgt ctgcagctgt
    11101 ggagaaaacc tgactcttct tagtaggaat tgtggtattt ctctcaataa gctttgtgaa
    11161 tactccaccc aacgtttcaa ttccaagaga aaggggagtt acgtcaagca acagaagatc
    11221 ctttacttct ccggtaagta ctcctgcctg aatagcagca ccaatagcaa cacattcgtc
    11281 aggatttatt cctttaaagg gctctttgcc aaaatacttt ttaactgcct cctgaacagc
    11341 aggtattctt gtggaaccgc caaccaaaag aatcttatca atcttatccg gtgtcagatc
    11401 cgcatccttc atagcttttc ttgtgggttc cattgtcatt tccaccagat ccgatgttat
    11461 ctcatcgaat tttgcccttg tcaaagtaat atcaaggtgt ttcggtcccg ttgcatcagc
    11521 tgtaataaaa ggaaggttta tattcgttgt agtaacgccc gaaagctcaa tttttgcctt
    11581 ttcagctgct tccttcagtc tttgcaaagc catcttgtca ttcctgaggt ctattccgtt
    11641 ttctctttta aaggtatcaa ccagataatt tattatcctg tcgtcaaaat catctccgcc
    11701 cagcctgtta tttccgctgg ttgccaatac ttcaaaaact ccgtcaccga tctccagtag
    11761 ggatacgtcg aaggttcctc caccgaggtc aaaaacaagg attttctgat cggtttcctt
    11821 gtcaagtcca taggcaagag ccgcagccgt aggttcgttg ataattctta aaacctcaag
    11881 accagcgatc ttacctgcat cctttgtagc ctgtctctgg gaatcactga aataagcagg
    11941 tacagttata accgcctgag taaccggctg gccaagataa ctctctgcat cagcctttat
    12001 tttctgaaga atcattgccg agatttcctg tggagtgtag ttcttatcgt caatcttaac
    12061 ccttttatcg gtacccatat ctctctttat agatataact gttctttccg gattggttat
    12121 agcctgcctt tttgctacct gtcccacaag cctctcattt gtttttgaaa aagcaaccac
    12181 agaagcagta gttcttcccc cctccgaatt tgggattaca acaggttctc cgccttccat
    12241 aacagccaca catgagtttg tggtaccgag atctatacct ataacttttg ccattaatat
    12301 catcctcctt aaaataaatt acatgaatta ttcaattgaa ccaagagaaa taagtcagtt
    12361 tgcaacctta accatactgt atcttatta
//
LOCUS       DAOOJC010000011        11654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_5062_length_11654_cov_3.783343, whole genome shotgun sequence.
ACCESSION   DAOOJC010000011 DAOOJC010000000
VERSION     DAOOJC010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 11654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11654
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5062_length_11654_cov_3.783343"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLA01_00620"
     CDS             complement(<1..248)
                     /locus_tag="PLA01_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPC99834.1"
                     /translation="MNSFKSLRTYPFYFIVFYLLMFMGFAVYSVFMPVYLDELGFDNS
                     NIGTLLSLGSFVGLFAQPLWGVVSDRSKSKNNVLRLLV"
     gene            complement(374..1762)
                     /locus_tag="PLA01_00625"
     CDS             complement(374..1762)
                     /locus_tag="PLA01_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HPC99835.1"
                     /translation="MTEYSMKELLEWNEKIEEIARSEGLNYYEQEFEICSYEDMIGYE
                     TYVGMPSHYPHWSYGKHYERTKTLHKYNLTGLPYEMVINSDPCIAYLMKDNTLLLQIL
                     TIAHVYGHNDFFKNNRLFKLGTRAKDTVEMFKNHANRIREYIQDPGIGYAKVEKILNA
                     AHAIKYQISRNIGVKRLTEKEKQEALLERYRRPRSEYPLLEPKAKEEEPPPDFKKIPI
                     EPEEDMLMFIMEYSKLSDWEKDIINIVREETLYFLPQIETKIMNEGWASFWHYTILNK
                     LELEQGLHIEFLKRHNQVLRPMMGQINPYYVGFKIFQDLKKRYADDPKKIFEVREVER
                     DQSFIRKYLTRELCEEMNLFEYMKLGSNYVITEVSDEEGWKKVRDTIANNVGLSGIPA
                     VNVLEWVRKDNTLVLEHRYDGREIELAYAYETLKHVVDLWDGKVVLATKIDNKRKLII
                     CDEQKKVALMDG"
     gene            complement(1787..2986)
                     /gene="yhbH"
                     /locus_tag="PLA01_00630"
     CDS             complement(1787..2986)
                     /gene="yhbH"
                     /locus_tag="PLA01_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YhbH"
                     /protein_id="HPC99836.1"
                     /translation="MAIFREYVNTGKDRAAEDRRRHKELVEESIKKNIGSIIAEESII
                     GQSKDKKIKIPIRGIKEYQFIYGNNSPSAGSGDGSEKRGDRIGQEKAGKKGDGSAGNQ
                     EGEDIYETEITIEELINYLFDDLNLPDIDRKKLAELESIRSFKNLGYQRKGIPPRLAK
                     KRSVIEKIKRKQGYLRNHRISEDSEMLEENREPSDTEDIEKKRFPFMEDDLRYRRVRE
                     DYKKDFNAVVICIMDVSGSMDQTKKYLARSFYFLLYQFIRLKYSNVDVVFVAHTTTAK
                     EVNENEFFHRGESGGTYISSGYEKALEIIEQRYNPASWNIYAFHCSDGDNWVEDNKKA
                     IELAEKHCDVCNLFGYGEITPGYYPSSSTIRAEYQKNIKRKNFSIITMSKKDDVLPGL
                     KKLLEEE"
     gene            complement(3045..4973)
                     /locus_tag="PLA01_00635"
     CDS             complement(3045..4973)
                     /locus_tag="PLA01_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HPC99837.1"
                     /translation="MEKRDLSSIIRKDREEKKRKKFEGTFLDYLDIVKEDPEVSMLAH
                     QRMYKLITEPGVKVIETDENPRLRRIYGNDVIKKYKFFDEEFFGIDKTIMKIVRYSHS
                     AAMAGEEARQVLYLVGPVGAGKSSLMEALKRALEMSPPIYALQGCPMREEPLHLVPKH
                     LRKEFEDVLNVRIEGDLCPVCRYRLKNEFNGEYERFPVVAVDFSIRSRKGIGVVPPVD
                     PNNQDTSVLIGSVDISKIDLYSEDDPRVLSLNGAFNVGNRGIVEFIEVFKNETEYLHT
                     MITATQEKSIPSPGKGSMIYFDGIILAHSNEAEWNKFKSDHTNEAILDRIVKIEVPYC
                     LELNEEIKIYEKILKKSKFDAHIAPHTIEIASMFAILTRLAPSNKVDPITKLKIYNGE
                     EVIEKGMTRKIDIFELREESPREGMTGISTRFIMKSLDTALSESVHNCINPISVLETL
                     VKAVKELAIGEEERERYLRFIQDTVRKEYNKILEKEITKAFIHSYREQAESLFNNYLD
                     HAEAFVNKTKIKDRNTGEELEADEKFLRSIEEQIGITDTAATGFRADVTAYMFYVLRN
                     GGKLDFTSYEPLKEAIEKKLMASVKDLSRVITQARVRDKEQSEKFDSMVEEMKRNGYC
                     DNCCNVILKYAANNLWKD"
     gene            5594..7378
                     /locus_tag="PLA01_00640"
     CDS             5594..7378
                     /locus_tag="PLA01_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99838.1"
                     /translation="MRAFYLAAKKFILCITSFLAGIFLFIGTISLSLSTTVMDSDFHG
                     KLFEKNNIYTEVHNEVNDLLHSIFTDYNNSPQLNEQQQEIFYVLQESISPKMVRTNLD
                     SIREGLFKYFRGDINFLPDIRLDTDSLPQDDTPQDVSAKKGVSSLETSDPDQVLSKIK
                     RVSLSAILRSINRSDILDRLLSVKLLYFVMDYIPKISVLVVAVLFILSLLLCKKSKEM
                     VKWLFGLLITSSFFNLIDAIAGFVLCYKIIPENINTLTMLIPLKSEVILSYLQDCILP
                     ISLYSMALSILIILISFAVLSSSRIVNKVSLSNIFRIKIPEKYLRIIEYSTLAVLFVL
                     FAGLLSFEVYSFKKEFISKNFTNVISKLTNANAYTEVVSAKDDTIYTLQIKLVNTKDN
                     SPVPGIQINVSGKTEIPEKYHNLTDITDETGSVKFTLGKGTFRLEFSSYRKPPEYVLP
                     SPFLFDLQSVGTTIVTVNLDECKETGEKTGITEIEFLNEDNLPVEKIELYVDNTNQPA
                     ENEEPSFEESGESSRIPDKYYSITNEEGIAVLKLPEGFYNVTISPDKFPKDYIIPESL
                     AVNCSADITARYTIRLVRKQTETPSPQN"
     gene            complement(7528..8463)
                     /locus_tag="PLA01_00645"
     CDS             complement(7528..8463)
                     /locus_tag="PLA01_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99839.1"
                     /translation="MDNKKEVLSTAGSESAKGLFDLIASKVPVNQGKEILSDLKDSLV
                     DNLHDKSKRAVDFIHSLKRDKIRNLERLLDVSRKDLKKSDKKTYIYMTDAGRTVIVDR
                     DIWGDPCTITVKEANKVLIRLVKFFNDIPYLFPVFNEKSLTGDIIFMAEDEIWYRDKD
                     EATLVLFKNDVPDFLNIKLNNVKKFNEKQLLKYLDNCKMLYLEKRLNNLLLVERKIGI
                     ENIVAIRKGYVIYKDTEYTVLLFDDLKNLFDEFTLKKMPDLSERAMDVLFSEDIVEYV
                     KSKGYSFENFKQDGEYLEYWQDIGNKRVCVMRCRF"
     gene            8919..9740
                     /locus_tag="PLA01_00650"
     CDS             8919..9740
                     /locus_tag="PLA01_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99840.1"
                     /translation="MILRKTAFTIILTTALLLSFSACQSGSTQNNAAPTDNGSSSESA
                     SNLDTTLSQENSSSNNNLANEANSTDYIEKLKSEGANEKEIESAELYVKRVMLQLNEI
                     QTFSNNSGFHLSAADPGLYNQDRDDSLKYLELSSKIDEKKAVYYILKLMDGFGSMDKA
                     FDEYLISLQLDIDLDMYITDKNSYGRLRSEKLSGIIEEDLITAEKIDEKAFESLQKLN
                     DANKNNYPNTGNEIYDPFDNSVPNVINPQPNIPQVDVPRPINPAEEINKKLSPYN"
     gene            complement(9968..10918)
                     /locus_tag="PLA01_00655"
     CDS             complement(9968..10918)
                     /locus_tag="PLA01_00655"
                     /inference="COORDINATES: protein motif:HMM:NF019913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99841.1"
                     /translation="MTTRKMKLLSTPLIFALVFSLISITCFAAYITDTYIYLKWQANG
                     GAKGFLGNAVTNTAQTPNKLGVYNHFEGGSIYRKNNVYEAYMVYGLIRNKWASLGWEN
                     SFLGFPLTDELETPRKDGRYNHFEGGSIYWKKGADKAYEVHGYIRSFWSWNGWETSQL
                     GFPTSDEYKINGKQASRFENGAIYWKKNKDGSVYIWPMMSAAKGSQSTNGTKLIVTYY
                     PVQFGTHVVITGSGFTPNKYVQIYLSRKYGPYSIEIIRVDSKGNLSYDSRQYGSKGCI
                     TNGNIEKVNGVITIWAQDSAGPVAIHSGYCYNVGNEVTLD"
     gene            11164..>11654
                     /locus_tag="PLA01_00660"
     CDS             11164..>11654
                     /locus_tag="PLA01_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99842.1"
                     /translation="MTFSIMLFSVTIGIIILISDPKTDWVRWLSVILFLAGLASFANE
                     LEDLVIPFLKHTFLTSEKTDTSILTINAFLTQMSEHLIAFCFIMFSISYSALFKTKTK
                     NILGCIFFAITLISFVYLPVMTNEQRRETGTYCHYYKVLSSWSIPLVLFGVSIILIAY
                     IKE"
BASE COUNT         3461 a         2549 c         1796 g         3848 t
ORIGIN      
        1 aaaaccagca atctcagaac attattcttt gattttgacc ggtcgctgac aacaccccat
       61 agcggctgtg caaacaatcc cacaaacgaa cctaatgaaa gcagcgttcc aatgttcgaa
      121 ttgtcaaaac ccagttcgtc aaggtagacc ggcatgaata cgctataaac tgcaaaaccc
      181 ataaacataa gtagataaaa aacaatgaaa taaaagggat aagttctaag acttttaaaa
      241 ctattcaata tgattctgcc ccttatgtac cagatttaat tcacagaaat attttcaata
      301 attattatat acccggtgaa attgattgta aacaggggcc cggcatcaaa ttcgatacat
      361 ggcccctttt ttactatccg tccatcaaag caaccttctt ttgctcatca catataatta
      421 actttctttt attgtctatt ttagtggcaa gtacgacctt tccatcccat agatccacaa
      481 catgctttaa agtctcatat gcataagcca actcaatctc cctgccgtca taacgatgct
      541 ccaagactag tgtattatcc tttctcaccc actccaacac atttactgca ggtataccgg
      601 aaagcccaac attattagct attgtatccc taaccttttt ccagccctct tcatcggaaa
      661 cttccgttat gacatagttg ctcccaagct tcatatactc aaaaagattc atttcctcac
      721 aaagctctct tgtcagatac ttcctaataa acgactggtc cctttcaact tccctgacct
      781 caaagatttt tttcgggtca tcggcatacc tttttttcaa gtcctggaat attttaaatc
      841 ccacatagta aggattgatc tgtcccatca tcggccttaa aacctggtta tgccttttta
      901 aaaactctat atggagtcct tgttccagtt ccagtttgtt taatatggta taatgccaaa
      961 aacttgccca tccctcattc attattttgg tttcaatctg aggaagaaaa tacaaagttt
     1021 cctctcttac tatgtttatt atatccttct cccagtccga aagcttggag tattccataa
     1081 taaacatgag catatcttct tccggttcaa tgggaatctt cttgaaatcc ggcggaggtt
     1141 cctcctcctt tgctttcggt tccagtaaag gatactcgct tcttggcctt ctgtatcttt
     1201 ccagaagggc ctcctgcttt tccttctctg tcagcctctt cactccaata ttacgtgaaa
     1261 tctgatattt tattgcgtga gcagcattca gtatcttctc aacctttgca tatcctatcc
     1321 ctggatcctg gatatactcc cttatcctgt tagcatggtt cttgaacatt tccaccgtgt
     1381 cttttgccct tgtccccaac ttaaaaagcc tgttgttttt aaagaaatca ttatgtccgt
     1441 atacatgggc aatggtcagt atctgaagaa gcagtgtatt gtccttcata agataagcta
     1501 tacacgggtc ggaatttata accatctcat agggcaaacc ggttaaatta tatttatgaa
     1561 gagttttcgt tctttcataa tgttttccgt aactccaatg aggatagtgg gacggcatgc
     1621 ctacataggt ttcatagcca atcatatcct catagctgca tatttcaaac tcctgttcat
     1681 aatagttaag cccctcagaa cgtgctattt cttcgatttt ttcattccat tccaataatt
     1741 ccttcatact gtattccgtc atatatccac ctccgaggtc agttaatcat tcttcttcca
     1801 ggagcttttt cagtcccggc agcacatcat cctttttgct catcgttata attgaaaaat
     1861 tctttctttt aatatttttc tggtattcag ccctaatggt gctgcttgaa ggataatagc
     1921 ccggtgttat ctctccgtat ccgaacaaat tgcaaacatc acaatgcttt tctgccagct
     1981 caattgcctt tttgttgtcc tccacccagt tatcgccgtc actgcaatga aaggcatata
     2041 tattccaact ggcagggttg tatctttgtt caattatttc caaggccttt tcatatccgc
     2101 tgctgatata ggttcctccc gattctcctc tatggaagaa ctcgttttcg ttcacttcct
     2161 tggcagttgt ggtatgtgct acaaaaacca catcaacatt ggaatattta agtcttatga
     2221 actgatacag caaaaagtaa aagcttctgg ccaggtactt tttggtctgg tccatggaac
     2281 cggatacatc cataatgcaa ataaccactg cattaaaatc ctttttgtag tcttctctga
     2341 ctcttctgta gcgtaaatca tcctccataa aaggaaaccg ctttttctca atatcttctg
     2401 tatcagaagg ttcccggttt tcctccaaca tctcagaatc ttccgaaatc ctgtgatttc
     2461 gcagataacc ttgttttctc ttaatctttt caataacaga tctctttttt gcaagcctgg
     2521 ggggtatacc cttacgctgg tagccgagat ttttgaagct ccttattgat tccagctccg
     2581 ccagcttttt tctatcaata tccggaaggt tcaaatcatc aaacagataa ttgatcagtt
     2641 cctcaatcgt aatctctgtc tcataaatgt cttcaccttc ctgattccct gcacttccgt
     2701 cccctttttt ccctgccttt tcctgcccta ttctgtctcc ccgcttttca cttccgtcac
     2761 cggaaccggc tgaaggggaa ttgtttccat atataaactg gtattccttt atgcccctaa
     2821 tgggtatttt gatcttttta tccttactct gaccgataat actttcctct gcaataatgc
     2881 tgcctatgtt tttcttaatg gattcttcca ccagctcctt atgccgtctt ctgtcttcag
     2941 ccgcacggtc cttgccggtg ttgacatatt ccctaaatat tgccaagacc aatcaccccc
     3001 tgctatgtct aaattccatg gccccacctt gtttacaaaa ggggttaatc cttccataaa
     3061 ttatttgcag catatttcaa aataacatta cagcagttat cgcagtatcc gtttctcttc
     3121 atttcttcca ccatgctgtc aaatttttcg ctctgctcct tgtctctgac ccttgcctgt
     3181 gtaataaccc tgctcagatc cttgaccgat gccataagct tcttctcaat agcttctttt
     3241 aaaggctcat agcttgtaaa gtccagcttg ccgccgtttc tcaaaacgta aaacatatat
     3301 gccgtaacat ccgccctaaa tcctgtcgca gcggtatcgg taatccctat ttgttcctca
     3361 atggacctta aaaacttttc atcggcctca agttcttctc ccgtattgcg gtccttgatt
     3421 ttggtcttgt ttacaaaggc ctctgcgtgg tcaagataat tgttgaagag actttctgcc
     3481 tgctctctgt aactatggat gaaagctttt gtaatctctt tttccaatat cttattgtac
     3541 tcttttctca cagtgtcctg aataaacctc aagtaccttt ccctttcttc ctcaccgata
     3601 gccagttctt ttactgcctt aaccagtgtt tccaaaaccg aaatgggatt gatacagtta
     3661 tgaacagatt ctgacaatgc cgtatccaaa gatttcatta taaacctggt ggaaataccg
     3721 gtcataccct ctctcggtga ctcttccctc agctcaaaaa tatcaatttt ccttgtcatt
     3781 cccttttcaa tcacttcttc accgttatat atcttaagct tggtgatggg gtcaaccttg
     3841 tttgagggtg caagccttgt caatatggca aacatggacg caatttcaat ggtatgaggt
     3901 gctatgtggg catcgaactt actcttcttc agtatttttt cgtatatctt aatctcctcg
     3961 ttaagctcga gacaataggg aacctctatc tttacaatcc tgtcaagtat ggcttcattg
     4021 gtgtggtcgg atttaaactt attccactct gcttcattgg agtgtgcaag tataattccg
     4081 tcaaaataaa tcattgagcc tttacccggg gatggaatcg acttttcctg ggttgcggta
     4141 atcattgtat gcaaatactc cgtctcgttt ttaaatacct caataaattc gacaataccg
     4201 cggtttccta cattgaatgc accgtttaag gacaataccc ttgggtcatc ctcggaatac
     4261 aaatctatct ttgaaatatc aacgcttccg ataagaaccg aagtatcctg gttgttcgga
     4321 tctaccggcg gaactacccc gatacccttc cgtgacctta tggagaagtc aacggctacc
     4381 accgggaacc tttcatattc gccattaaac tcatttttca gtctatagcg gcatactggg
     4441 cacaagtccc cttctatacg aacatttaac acatcctcaa attcttttct caagtgtttc
     4501 ggaacaagat gcaaaggttc ttccctcata ggacacccct gcagtgcata aatcggcggg
     4561 ctcatctcaa gagcacgttt caaggcttcc attaatgatg acttaccggc accaaccggt
     4621 ccgaccaaat agagcacctg ccttgcttct tctcctgcca ttgcagccga atgagaatac
     4681 ctgactattt tcattatcgt tttatcaatg ccaaaaaatt cctcgtcaaa aaacttgtat
     4741 ttttttatta catcatttcc atatatcctt ctgagccttg gattctcatc tgtttcaatt
     4801 actttaactc ccggttctgt aatgagttta tacatccttt gatgagcaag catcgaaacc
     4861 tcaggatctt cttttacaat atcaaggtag tcaagaaagg taccctcgaa cttttttctc
     4921 tttttttcct ctctatcctt gcgaataatg cttgataaat ctcgtttttc catgcttatt
     4981 cccccttttc tttgaatatc ctttgatttt tgcagtattc ccggtttata gcccaacata
     5041 accattatat tgctttataa ataagaagtt gattaatata gccctttaga tatatatatc
     5101 cattgtatac tttcaattaa catatataag aatataaatt tttccgtggt ctctactttg
     5161 aagtaagtat catactttca tgtatattac ccaggtgaaa ataaaatact caggattaac
     5221 cgggatacaa aaccgatatt gaccatgcat aaactgtcct gcatgcagtc ttctgtaaga
     5281 tattatctgc aacaggtttc caaggaacaa gatacactaa ttttccaatg caaaaaaagt
     5341 aagccatgtg tttttaaaga attgctacag ttttattata acatatcagt atgtaaatag
     5401 ccatattatt tatttagcga attctgtcag ggtgcaaatt acctgatgaa gaaaaattcg
     5461 agaaaaaacc tgttatattt ttcttaattt ttttataaag tttaatgata taatagtata
     5521 tggtatgaat ccgatttgtc tgatcttaac aaggttttat ccacgttaat caacattttc
     5581 aaggaggtat gttatgagag ctttttattt ggcagctaaa aaattcattc tttgtataac
     5641 atccttcctc gccgggattt tcctctttat aggaacaata tccctaagtc ttagtacaac
     5701 cgttatggat tcggattttc atggaaaatt atttgaaaaa aacaatattt ataccgaggt
     5761 tcacaatgag gtaaatgatc tattacatag catatttacc gattataaca attcgccgca
     5821 gcttaatgaa caacaacaag aaatatttta cgttcttcaa gaatctatat cgccgaaaat
     5881 ggtaagaacc aatctcgatt ccataagaga aggccttttt aagtacttca ggggtgatat
     5941 aaacttcttg cccgatatta ggcttgatac agattcgttg cctcaagatg acactccaca
     6001 ggacgtttcg gctaaaaaag gggttagttc cctggaaaca tccgatcccg accaggtttt
     6061 gagcaaaata aaaagagtaa gtctcagtgc catactaagg tcaattaatc gaagcgatat
     6121 tcttgaccgg ctcctttcgg taaaattgct ttatttcgtt atggattata ttcccaaaat
     6181 atccgtactg gttgttgctg ttctgtttat tctttccctt cttctttgta agaagtcaaa
     6241 agaaatggtt aaatggttat ttggattact gattacaagc agttttttta accttataga
     6301 cgctattgcc ggtttcgtac tttgctacaa aatcattccc gaaaatatca atacattaac
     6361 aatgctcata cctttaaaat ccgaagtaat cttatcctac cttcaggatt gtattctccc
     6421 catatccctc tatagtatgg cactgagcat acttattatt ctaatatcct ttgccgtgtt
     6481 atcttcaagc cgcattgtca ataaggtatc gcttagcaat attttccgta taaaaatacc
     6541 cgaaaaatac ctgagaataa ttgaatattc aacccttgcc gtattgtttg ttttatttgc
     6601 cggacttctt tctttcgagg tgtattcttt caagaaggag tttatctcaa agaattttac
     6661 aaacgttata tcaaaactaa ccaatgcaaa tgcgtacacg gaagtagttt ctgcaaagga
     6721 tgacacaata tatacacttc agataaaact cgttaacaca aaagacaact caccggtacc
     6781 cggtatccaa ataaatgtca gcggcaaaac agaaattccc gaaaagtatc acaatttaac
     6841 cgatataacc gacgaaacag gttcggtaaa attcaccttg ggcaagggaa ccttcaggct
     6901 ggaattctct tcataccgta aaccgcccga atacgtgctg ccgtcaccct ttttattcga
     6961 tttgcaatct gtaggtacca ctattgttac cgttaatctg gacgaatgca aagaaactgg
     7021 agaaaaaaca ggaatcacgg aaattgaatt tctgaacgaa gacaacctgc cggttgaaaa
     7081 aatagaactc tatgttgata atacaaatca acctgcagaa aatgaggaac cttcttttga
     7141 agaaagcggt gaatcttcaa gaatccctga caaatactac tccataacaa acgaagaagg
     7201 catagccgtg ctaaaacttc ctgaaggttt ttataatgta acgatttcac cggataaatt
     7261 tcccaaagac tacataattc ccgagtcctt ggcagttaac tgttctgccg acattactgc
     7321 aaggtacaca atacgccttg taagaaagca aacggagaca ccgtctccac agaattgaac
     7381 caatattcta aaaaataaag ggactgccgc aactccacag tcccttttac acaatactaa
     7441 ttttcccagc aatatattat aagtacataa tagtgcggtt atatccgtgt cataataatt
     7501 acaagtcagt ttcgattctt aaaatgctca gaatctgcac ctcataacac aaaccctctt
     7561 attgcctata tcttgccagt attcaagata ttctccatcc tgcttgaaat tttcaaagga
     7621 atatccttta gacttaacat actccacaat atcttctgaa aaaagaacat ccattgccct
     7681 ttcgcttaag tcgggcatct tcttcaatgt aaactcgtca aacaggtttt taagatcgtc
     7741 aaaaagaagt acggtatatt cggtgtcctt atagataaca tatcccttac gtattgcaac
     7801 aatgttttct ataccgattt tcctttcaac caacagtaga ttgttcagtc ttttctccag
     7861 atacaacatt ttgcaattat caaggtattt gagcaactgt ttttcattaa atttcttcac
     7921 attattcagt tttatattca agaaatcagg gacatcgttt ttaaacaaaa ctaaagtcgc
     7981 ctcatccttg tcgcggtacc atatttcgtc ttcagccata aatatgatat cgccggttaa
     8041 agatttctcg ttaaacacag gaaacagata gggaatatca ttgaaaaact taacaagtct
     8101 tataagaact ttgttagcct ctttaaccgt aattgtacac ggatctcccc atatatccct
     8161 gtcaacaata accgttctgc ctgcatcggt catgtagata taggtcttct tgtccgactt
     8221 cttcaaatcc tttctggaaa catcaagaag tctttcaaga tttctgatct tatctctttt
     8281 aagtgagtgt ataaagtcca cagctctttt tgacttatca tgaagattat ccactaagct
     8341 gtcttttaaa tccgacaaaa tttctttgcc ttgatttaca ggaaccttag atgctattaa
     8401 atcaaataaa ccttttgcac tttcactacc tgccgtgctc aatacttcct ttttattatc
     8461 caagaacacc accccttttt atacgaacga gaaataaaga aggtaagaat agacaatatt
     8521 gcgaagacta atacattttt tattattatt gttatgcaga atatataaaa tatattatca
     8581 atattattat accttataac actgaatatg ccaataccta aaacccataa aatataacaa
     8641 ataattatat tttgaaaaat gccaaacaat ctgatacatt taatatctta ataattaata
     8701 atatatgtta atatccctgc actaatgtct ttattttatg atccttggat ttttatggca
     8761 atatccggag cttttactcg ccatatattg gggtattcaa tactaattta taattaaaat
     8821 tgaaggtaat aaaaaggctt tcttccccta aacatttctg ctagaatagt agatggtaaa
     8881 attgacaatc tttattatga aatgggggaa gtaacaaaat gattttaaga aaaacagcat
     8941 ttacaatcat acttaccaca gcactgcttt tatctttttc cgcttgccag tcaggcagta
     9001 cacagaacaa tgccgcaccc acggataacg gtagttcctc cgaatcagcc tcaaatttag
     9061 ataccacttt atcacaggag aatagcagca gcaataacaa ccttgcaaat gaagcgaatt
     9121 caacagacta tatagaaaag cttaaatcgg aaggggccaa tgaaaaagaa attgaatcgg
     9181 cagaattgta tgtaaaacgg gtaatgcttc agcttaatga gatacagaca ttcagcaaca
     9241 attccggttt tcatctgtcc gcagccgacc ccggtttata caatcaggat agggatgatt
     9301 cattaaaata tttagagctt tcttctaaaa ttgatgagaa aaaagccgta tactatattc
     9361 ttaaattaat ggacggtttt ggcagcatgg ataaagcatt tgacgaatac ttaatttcac
     9421 tgcaactgga tatagattta gatatgtata taaccgataa gaactcctat ggccggctac
     9481 gttcagaaaa gctctcgggg ataattgaag aggatttgat taccgcagaa aagattgatg
     9541 aaaaggcttt cgaaagcctt caaaagctta acgatgcaaa taaaaacaat tatcccaata
     9601 cggggaatga aatctatgac ccctttgata attcggtacc gaacgtaatc aatcctcagc
     9661 cgaatatacc tcaagttgac gttccccgcc cgattaatcc ggcagaggaa ataaataaaa
     9721 agctttctcc atataattaa aacaacataa ttaatttgaa aaaacccgtt ttctttgcgg
     9781 gttattttta tcttttctaa tcatttcata gaaatccagt atcaaaaggg tatgaattcg
     9841 aaaactcgta gagcttcgaa attctatatt ggaggccaag cattttctgc tttgcctcca
     9901 attgattttc taccagaccg atagggtcaa attaattctc taagaaacta atctccccat
     9961 ccgtttctta gtcaagagtc acttcattac ctacgttata gcaataccct gagtggatgg
    10021 caacaggacc tgcactatct tgtgcccaaa tagttataac cccgttaact ttttcgatgt
    10081 taccatttgt gatacacccc tttgaaccat actgccttga atcataggac aaattacctt
    10141 tactgtcaac acgtataatt tctatggaat aaggtccata ttttcttgac aaataaattt
    10201 gcacatattt atttggtgta aatcctgaac ctgttatcac aacatgagta ccaaattgaa
    10261 ccgggtaata tgttacaatc agttttgtcc cgttcgtcga ctggcttccc tttgctgcag
    10321 acatcatagg ccatatatat acagaaccat ccttattctt tttccagtaa attgctccat
    10381 tctcaaaacg acttgcttgt ttaccattta ttttgtattc atcactggtt ggaaaaccta
    10441 attggcttgt ctcccaccca ttccacgacc agaaactcct gatataacca tgaacttcat
    10501 aggctttgtc tgcacctttc ttccagtaaa tagaacctcc ctcaaaatgg ttatatctcc
    10561 cgtcttttct gggtgtttca agttcatctg ttaaagggaa acccagaaaa ctattttccc
    10621 agcctaaact ggcccactta ttccgtatca gaccatatac catgtatgcc tcataaacgt
    10681 tgttttttct atagatggag cctccttcaa agtgattgta aacgccaagt ttattgggtg
    10741 tttgagctgt gtttgttaca gcattaccca gaaaaccttt tgcaccgcca tttgcttgcc
    10801 atttcaaata gatataggta tcagttatat acgccgcaaa acatgttata gaaattagtg
    10861 aaaaaactaa agcaaatatt aatggtgtac tcaaaagttt catttttcta gtggtcataa
    10921 taaactcctc cttataattt atcacttgat ttttactatc tgaaaaaaca agaagcataa
    10981 attgggaata ttccaccttg tcgacgcgtc ttttatatac tcataatata taaaaaagtt
    11041 aaatctgata agtattaaat tttttagtac ctttgtatta aaaacataaa aagtatggta
    11101 aaatattgct taacaataat aaaaaaaaac aatactactt actttatttg atgaggttag
    11161 cgaatgacgt tttcaattat gcttttttca gttacaattg gcatcataat ccttatatcg
    11221 gatcctaaga cagactgggt caggtggctt tctgttattc tgtttcttgc cggtcttgct
    11281 tcctttgcca atgaattgga agaccttgta atcccatttc taaaacatac ctttcttact
    11341 tctgagaaga cggatacgag tattttaact attaacgcct tccttactca aatgtcagaa
    11401 catttaatag ctttttgctt cattatgttc agtattagct attctgcttt gtttaaaaca
    11461 aaaacaaaaa acatattagg atgcatattt tttgctatta ctcttatctc atttgtatat
    11521 ttacctgtta tgacaaatga gcaacggagg gaaacaggta cctattgtca ttattacaag
    11581 gttttatcaa gctggtccat tcctttggta ctgttcggtg tttccataat actcatcgct
    11641 tatataaaag aaaa
//
LOCUS       DAOOJC010000012        11645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_5066_length_11645_cov_3.291458, whole genome shotgun sequence.
ACCESSION   DAOOJC010000012 DAOOJC010000000
VERSION     DAOOJC010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 11645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11645
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5066_length_11645_cov_3.291458"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="PLA01_00665"
     CDS             complement(<1..1173)
                     /locus_tag="PLA01_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99843.1"
                     /translation="MKPVAKDKKIFVILGVLVVFALYYGFIYTKQKKDIKEIKRNIEV
                     YYDLSAAYDDARLKVENMDSEIKILKQKLSVLRSVFPPQLNHDEVLMILKEISERSGL
                     EIDSLTFSGITEVSYGKKDGEGSSKTNNNSLSVSVNSPKIKGFMAEMGIDLGGFEENG
                     QNEIKGIRDGKGYKLEVSVNAKGNNTNLKDFIRTIETLKNKVSFESITIESAGGEQLS
                     VSMVLNLYGIMERGAVSKNKLLDISWEPMNSADKNDIFIPYEGYKEQIAAKDDNDEKQ
                     VNDSSESEVDEYDFTMRVMHYGENMSPPTVTLVGKSISIKRDEFKIPIVYGDNRFDEK
                     VEIYLEQKNGKYLCRLKTEHDSFPGGSTELSGFEPKGDAIRVLIDSSRRTSANDTSG"
     gene            complement(1178..1786)
                     /locus_tag="PLA01_00670"
     CDS             complement(1178..1786)
                     /locus_tag="PLA01_00670"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HPC99844.1"
                     /translation="MKDLNLVPKEYLLKNRRKKKVFLYSIAGVFLASMLAVFVTEPLL
                     TGYNLRRKFSILDKQIKESVNYVEIEREFSQLQNVYMQREDEAKALMSMGFSITSLIE
                     TIEKYMPEKLFTMNLSVNNTSTGTNVILRGVSASENELASFISHLRNSSVFDSVELTT
                     VNRKVTNVSTSGTYDTGSSNGVKDLQVESYDFEINLIINSGK"
     gene            complement(1806..2312)
                     /locus_tag="PLA01_00675"
     CDS             complement(1806..2312)
                     /locus_tag="PLA01_00675"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPC99845.1"
                     /translation="MPRLGNKGFTLAEMMAAIIILGIIIVPVSLAFYTGYRNYFIENE
                     EMTAQEKAREIIEMIITDLRMYENEHTVADNDSNTLTIKDSTHFPGEEIVYTYSPEQK
                     CLFRNGIELFAEVDSPEVTDFAIEETRQADYDSSIIIIEVSVKYNRSDTVKLRGSFRR
                     KFDITSLE"
     gene            complement(2395..3474)
                     /gene="pilM"
                     /locus_tag="PLA01_00680"
     CDS             complement(2395..3474)
                     /gene="pilM"
                     /locus_tag="PLA01_00680"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPC99846.1"
                     /translation="MKKYIVIDMGSSKIKIVHGGMEKDKIVVYEYDIVDTPEGSIQDG
                     KIINLDVIVNVIKGVLKKNRIKGKRLVLGITGTGIITRDIQIPKSTDEEIEKILEFEA
                     QQHFPVLLEDYILDFKVQEEIENDEGVFFRVLLVAVPKEQIDRYMQIHKKIDMEIEAI
                     DIPANNFFKLFFGMDYLDRRFGNKELPDEFAVLDIGSRATEVHIFSAGKLKFSRILLN
                     GSMDIDELIANSFNVDFRKAEDLKIKSGRLIDENDTLDESDEALMMSNIIKPAVSNIM
                     NNVNRFFEFYNSRKTGNSLDKIFICGGGSRLFGMDTFLSSYFGMPVEKLLPFDNIVYK
                     GRKKQEEFIEDYLYLAGIIGGLLRG"
     gene            complement(3583..3852)
                     /locus_tag="PLA01_00685"
     CDS             complement(3583..3852)
                     /locus_tag="PLA01_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HPC99847.1"
                     /translation="MIQVKNYMEEVVFSMIKEIIGEINVCTCEKCMMDIAAIALNDLP
                     PKYIVSEKGELYSKVNSLKQQFEVDVISAVTKAAVLVKRNPRHEK"
     gene            complement(3986..4819)
                     /locus_tag="PLA01_00690"
     CDS             complement(3986..4819)
                     /locus_tag="PLA01_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPC99848.1"
                     /translation="MMSLSMFSQEMMPFYYILFGILGLLVGSFLNVCIYRIPKNETVI
                     TTPSHCMKCGHRLTAIDLIPVLSFVFLRGKCRHCGDKISPRYAIVELLTSLTFVLLFH
                     KFGLTVDFLASAYLMSILIVVFFIDIDHMIIPDGLVIAGLIGGGILFIYNFFRPVAFY
                     GDRNWWNPLLGGVIGFGILLLIGVVGNAVYKTDEAMGGGDIKIFAPIGIFLGWKMTGM
                     ALLASFLFGGVLGFILLILNKNKRRSTFAFGPFIVLGTFVTYLFGWDVLNWYLGTLNI
                     Y"
     gene            complement(5052..5447)
                     /locus_tag="PLA01_00695"
     CDS             complement(5052..5447)
                     /locus_tag="PLA01_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPC99849.1"
                     /translation="MLKLLNKKLRNKGGFTLTELIVVVAILGILVAVAVPTVIGYIDD
                     AKESADLSNAKTLENAIARLMTKGTIQRDSGEISTDDATIISEILKEINPIPECQRDG
                     YSFVLDKATAKVTAEKDFTETEGYLILEN"
     gene            complement(5468..6682)
                     /locus_tag="PLA01_00700"
     CDS             complement(5468..6682)
                     /locus_tag="PLA01_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPC99850.1"
                     /translation="MAVYSYKAKTASGETAAGKLDAVDRDTVVDLLRSRGLLPMEISE
                     VSVLDREIKLKKNRAIALGDISLFCKQFYTMINSGVTIIGSLDLLRKQTENAHLAKIL
                     TRMYEDVQKGVSLSDSMKQDKDAFPAIVISMIEIGEISGSLDTVLDRLSVYIEKENRV
                     KQKIKTAMVYPKVLSVIAIVVIAFMMTFIVPNFVAMFKNIGGQLPLPTRILLWISNLF
                     KNPWFLASLAIIIPASVYSFRKFKKSDKGRYFISWISLKLPVIGKNTRKLIASRFSRS
                     LGLLLRTGVPLIQSLEVVENVLGNVIVSKALEKVKEDIKRGSGLAEPLEGIGIFPLMV
                     NHMISIGEEAGSLDSIVEKVADFYDDELDASISKMVTMLEPLMIVVIALMVGGIVIAM
                     ILPVFSMYKGLR"
     gene            complement(6730..7785)
                     /locus_tag="PLA01_00705"
     CDS             complement(6730..7785)
                     /locus_tag="PLA01_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPC99851.1"
                     /translation="MNAKELFRSVSEQGGSDLHITVGAPPTIRKNGKLVKLGEEALTF
                     DNTEEFVKQIVNEAQMKELLKSGQVDFSFSLSGIGRFRVNAFKQRGAFGAAIRLVNSS
                     VISIKELGLPDIVSELSRKTKGLILVTGPTGSGKSTTLASMIDQINEERECHILTLED
                     PIEYLHRHKKSIVNQREIGNDAVSYAKALKAALREDPDVILVGEMRDIDTISIAITAA
                     ETGHLVLSTLHTVGAASTIDRIIDVFEPYKQQQVRVQLSVTLQGVISQQLLRRQDVPG
                     RVAAVEVMIATPAVKNLIREGKTHQIDSLIQTGAKFGMITMDSSLVDLYKKGMIRYED
                     ALAYASSYENVKRLLGS"
     gene            complement(7810..9498)
                     /locus_tag="PLA01_00710"
     CDS             complement(7810..9498)
                     /locus_tag="PLA01_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPC99852.1"
                     /translation="MEKHIRKKLGDLLIESGLITKEQLDNSLRLQKTTGKKLGELLIS
                     EGIVTQEDIIQVLEFQLGIPHVLLQKYSIDQTACLLIPENLARRYELIAIAQHDGVLT
                     VAMSDPLNLLAIDDIEIYTGLEVQPVIASSEDITRAIDRYFGNQHALKAVEEFKKKSE
                     SLSKIEPGKTDRMGEDLNNAPAVKLVNSLIEQAVRNRASDIHIEPSENEIKVRFRTDG
                     QLHEVMRIETAIMNTLATRVKIISGMDITEKRYPQDGRATVKVDGKEYDLRVSVFPIV
                     FGEKIVIRISDKNAFILQKERLGLTDEDMVMFDGILKNPYGMILVTGPTGSGKTTTLY
                     TALNEINKPDINIITIEDPVECIIEGVNHSQINYKTGLNFANSLRSVLRQDPNVIMIG
                     EIRDLETAQIAVRSAITGHLVLSTLHTNDAPSTVLRLIDMGIEPFLVASSLIGVIAQR
                     LVRKICPNCKRERVADNDIVSLLGLKEPVKIYYGSGCAMCNGTGYRGRIGVYEVMSIG
                     KKHKELINRRCLEDELRECSIANGMKTLKENAKILVLKGITTVEEMVRVSYSNT"
     gene            complement(9696..10580)
                     /gene="aroE"
                     /locus_tag="PLA01_00715"
     CDS             complement(9696..10580)
                     /gene="aroE"
                     /locus_tag="PLA01_00715"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPC99853.1"
                     /translation="MEIDVRVTGKTKLLGLMGNPVEHSISPQLHNTLSSILGHDIVYV
                     PLKVEKQDIGTVVNALKRLEFIGFNVTVPYKREIMKYIDENTKDAILMGAVNTVKNID
                     GRLFGYNTDAEGFARSFREEAGVGLGGKKVVLIGAGGVARSIAVKLAKEGAEKISLVN
                     RTTLKAVELADVVNENIREVVQVYNFNDKTFMMAFEESDIIINTTSAGMYPEVGNTPI
                     KAKKCFNKNQIVYDVIYNPVKTKFLMDAEKAGAKVINGLGMLFYQGISAYEIWTGVKF
                     SEEDLSFIYDSFKKILRA"
     gene            complement(10679..11191)
                     /locus_tag="PLA01_00720"
     CDS             complement(10679..11191)
                     /locus_tag="PLA01_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HPC99854.1"
                     /translation="MLEKFYPDLKVDKIQDIDLDFLIKNNIKGCVLDIDNTLVPEHAQ
                     EADEKALEWIEKIKKKGFKACIVSNASQKRVVKFNERLKLYAIHRASKPGRKSFLKAM
                     ELMGTKPSETAVIGDQIFTDIYGGNRLNMFTILVQPIDRREFIGVRIRRAFEGIVLAR
                     YYRTLQKSRK"
BASE COUNT         3359 a         2687 c         1824 g         3775 t
ORIGIN      
        1 accggaagta tcattggccg atgttctgcg ggacgaatcg attagaaccc ttatcgcatc
       61 acctttaggc tcaaatcccg ataactccgt gcttcctccc ggaaacgagt catgttcggt
      121 ttttaatctg cacagatatt tgccgttttt ttgttcaagg tatatctcca ccttctcatc
      181 aaaacggttg tctccgtaaa caatcggaat cttgaactca tctcttttaa tacttatgct
      241 ttttcccacc agtgtcaccg tcggcggtga catattctca ccgtagtgca tgactctcat
      301 ggtaaagtca tattcatcaa cttccgattc cgaactgtca tttacctgct tttcatcatt
      361 gtcatccttt gcggcaatct gttctttata gccctcataa ggtatgaata tgtcattctt
      421 gtctgccgaa ttcataggtt cccatgatat atccagcaat ttatttttgc tcacggcacc
      481 cctttccata attccgtata aattgagtac catgctcact gaaagctgtt cgccgccggc
      541 actttctatt gtgatcgact caaaactcac tttattcttt aatgtttcta ttgttcttat
      601 aaaatccttt aaattggtat tgtttccttt ggcattaaca ctaacttcca atttataacc
      661 ttttccgtca cgaatacctt ttatctcatt ttgtccgttt tcttcaaaac cgcctaaatc
      721 tattcccatt tctgccatga acccctttat tttagggctg tttaccgata cactcaacga
      781 attattatta gttttggaag aaccctcccc gtcttttttt ccatatgaaa cctccgtaat
      841 acccgaaaag gtcaagctgt caatttccaa ccctgacctt tcagaaatct cctttaaaat
      901 catcaatacc tcgtcatggt ttaactgagg aggaaacaca cttctaagca cggaaagctt
      961 ctgctttaat atctttattt cactgtccat attttccact ttaagccttg cgtcatcata
     1021 agccgcactc aggtcataat acacctcaat gttcctttta atttccttta tatctttttt
     1081 ctgttttgta tagataaaac cgtaatacag tgcaaatacc acaagcactc caagaattac
     1141 gaatattttt ttatcttttg caacaggctt cattttctca cttcccggag ttaataatca
     1201 ggtttatttc aaaatcatag ctttccacct gtaaatcttt tacaccgttt gacgagccag
     1261 tgtcataagt cccagaagtc gaaacattgg tcaccttcct gttaactgtt gttagttcaa
     1321 cgctgtcgaa cacactgctg ttccttaaat gactgataaa agaagccaat tcattttcag
     1381 aagcggatac acctcttaaa atcacatttg tacccgtact tgtattattt acagacagat
     1441 tcattgtaaa aagcttttca ggcatatact tttcgatggt ctctatcagg gaagttatac
     1501 taaatcccat tgacatcaat gctttggctt catcttccct ttgcatataa acattctgta
     1561 attgggaaaa ctccctttca atttcaacat aattgaccga ttcctttatt tgtttgtcaa
     1621 gaatactaaa tttgcgtcgg agattgtatc cggtcaacaa aggttcggtc acaaagacag
     1681 caagcattga agcaagaaag actcctgcaa tgctgtacaa aaataccttc tttttccttc
     1741 tattttttaa taaatattcc ttaggaacaa gattcaaatc cttcaaagca agtccccacc
     1801 ttaagtcatt caagacttgt aatatcaaac tttctcctga aacttccccg aagtttgact
     1861 gtgtcgcttc tgttgtattt aaccgaaacc tcaatgataa ttatgctgct gtcatagtcc
     1921 gcttgtcttg tttcttcaat tgcaaaatcc gttacttcgg gagaatcaac ttcagcgaac
     1981 agttcaatac cgtttctgaa caagcacttt tgctcaggag aataggtata tacaatttcc
     2041 tctcccggaa aatgggttga atccttaatt gtcaacgtat ttgaatcgtt atcggcaacc
     2101 gtatgctcat tttcatacat tctcagatca gttataatca tttcaattat ttcccttgcc
     2161 ttctcctgtg ccgtcatctc ttcgttttca ataaagtaat ttctgtatcc cgtataaaac
     2221 gcaagggaaa ccggtacaat tattatcccc agaataataa ttgcagccat catctcagcc
     2281 aatgtgaatc ccttatttcc caaccgcggc ataaaaacct ttcccttcaa aatattatcc
     2341 cgggctgcat tcgaattcct gtacgtcagt cagaataaca cgttaaattt ataattaacc
     2401 tcttaaaagt ccgccgataa taccggccag ataaaggtag tcttcaatga attcctcctg
     2461 cttttttcga cctttgtata caatgttgtc aaaaggtaaa agtttttcaa caggcatacc
     2521 aaagtatgaa ctgagaaatg tatccattcc gaaaagcctg cttccgccgc cgcatataaa
     2581 aattttatcc aggctgttgc cggtttttct tgaattgtaa aattcaaaga acctgttaac
     2641 gttattcatt atattgctca cggcgggctt tattatattg ctcatcatca aagcctcatc
     2701 agattcgtca agggtgtcat tctcatcaat taacctgccc gactttattt tcaaatcctc
     2761 agcttttcta aaatccacgt tgaagctgtt tgcaatgagt tcatctatat ccatgcttcc
     2821 gtttaacaat atcctgctga acttaagctt tcctgccgaa aaaatatgaa cttccgtcgc
     2881 cctggacccg atatccaaaa ctgcaaattc atcaggaagc tccttgttcc caaacctcct
     2941 gtcaaggtaa tccatcccga agaaaagctt aaaaaagttg ttggcaggta tatctatggc
     3001 ttcgatctcc atatcaattt ttttatgaat ttgcatgtac cggtcaatct gctccttcgg
     3061 aactgcaaca agcaaaaccc tgaaaaagac tccttcatca ttttctatct cttcctgaac
     3121 cttaaagtcc aatatataat cttccaaaag aacgggaaaa tgctgttgtg cctcgaactc
     3181 aagtattttt tcaatttctt cgtcagtgga tttaggtatt tgaatatctc tggttatgat
     3241 accggtgccg gttattccaa gcaccaatct ttttccttta attctgtttt tcttcaatac
     3301 acccttgatt acgttaacaa taacatcaag gttaataatt tttccgtcct gaatgctgcc
     3361 ctcaggggta tcgacaatat catattcgta tacaacaatt ttatcttttt ccattccgcc
     3421 atgaacaatc ttgattttac tgcttcccat atctatgaca atatatttct tcattagcat
     3481 ggatactcac ttccataata taaaatgggt aaaagccctg ccttcagata aaccaaacat
     3541 ataacaatgc ctgactgctc tatggaatgg cactttaatc attcatttct catgtcttgg
     3601 atttcttttg accaataccg ctgcctttgt tacagcagaa ataacgtcaa cttcaaattg
     3661 ctgctttaat gaattgactt ttgagtaaag ttcacccttc tcacttacta tgtatttggg
     3721 aggtaaatca tttagggcaa tcgcagctat atccatcatg catttctcac aggtgcacac
     3781 attgatttct ccaataattt cctttatcat ggaaaaaact acctcttcca tatagttctt
     3841 tacctgtatc actttactta cctccaccat tattaccgta tggtaattaa taaccaggtt
     3901 aaaccccata atatatatcg gtaataagaa aattatatat tagacttttg cataatacgc
     3961 ccgaattatt caccttggct cggatttaat atatgttcaa tgttcccaaa taccaattaa
     4021 ggacatccca gccaaacaga tatgttacaa aggtcccgag aacaataaac ggaccgaagg
     4081 caaaggttga ccttctcttg tttttattta aaatcaacaa tataaagcct aaaacaccgc
     4141 caaataaaaa tgatgccaat aatgccatac ccgtcatctt ccatccgaga aaaatgccaa
     4201 tgggtgcaaa tatcttaata tctccgccgc ccattgcttc atctgtctta taaaccgcat
     4261 ttcccacaac cccaataagc aacaaaatac caaatccaat aacgcctccc aaaaggggat
     4321 tccaccaatt ccggtctcca tagaatgcaa ccggcctgaa aaaattgtat ataaacagta
     4381 tacctccgcc aatcagccct gctatgacaa gaccgtcagg tattatcatg tgatcaatgt
     4441 caatgaagaa tacaacaata agtatggaca taagatacgc cgatgccaaa aaatcaaccg
     4501 taagaccgaa tttatgaaat agaagcacaa aagtcaggga cgtcaggagc tcaacaattg
     4561 catacctggg agatatttta tctccacaat gcctgcactt tccccgcaag aagacaaagc
     4621 ttaaaacagg aatcaaatct atagcggtaa gtctgtgccc gcatttcatg cagtgggaag
     4681 gagtggtaat aacagtctcg tttttcggta ttctgtaaat acatacatta agaaacgatc
     4741 ccaccagtaa cccgaggata ccgaacagga tgtagtaaaa cggcatcatt tcctgactaa
     4801 acatgctcaa gctcatcata aggctcataa tattcctctt tctcatatgt tttttatata
     4861 attatacatt ataagaatag tcttttaaag tgttttttaa ttttgagggg tgcatgtaac
     4921 aattagcacc ctgttataaa tatgtaagta cagggccggt ggcgaaaccc tgtacctttt
     4981 atttcggcat ttatcaagta aaatgattaa cagtgaggga atccggatac cataaagtat
     5041 tcatttaata gttaattctc gagtatcaaa tatccctctg tctcagtaaa atctttctca
     5101 gcagtgactt tcgcagttgc tttgtccaaa acaaaactat agccgtcacg ctggcattcc
     5161 ggaatcggat taatttcttt taatatttca gatataatag tggcatcgtc agtggaaatt
     5221 tctccggaat cccgttgaat agttcctttt gtcatgagtc ttgcaattgc attctccaga
     5281 gtttttgcat tggacaggtc agcactttct ttggcatcat caatataacc gataacggtt
     5341 ggcacggcaa cagcaacaag tattccgaga atagctacaa cgacaatcaa ttcggtaagt
     5401 gtaaatccac ctttgtttct tagttttttg tttaatagtt ttaacataaa aaccctcctt
     5461 aattggatta cctgagacct ttatacatag aaaacacagg cagtatcata gcgattacga
     5521 tacctcctac cataagtgca attacaacta tcataagtgg ctcgagcatt gtaaccatct
     5581 tgctgattga tgcatccaac tcatcatcgt agaaatccgc caccttttct acaatggaat
     5641 caagagaacc tgcttcctct ccaatgctaa tcatatgatt taccatcagg gggaagatcc
     5701 caattccctc aagaggctct gccaaaccgg aacctctctt tatatcttct tttacttttt
     5761 ccaatgcctt tgatacaatt acattaccta gaacattttc caccacctcc aatgactgaa
     5821 taagaggtac accggttctg agcagcaaac cgagcgagcg gctgaacctg gaagcaatta
     5881 actttcgggt gtttttcccg ataacaggta attttaatga aatccagctg ataaaatatc
     5941 gtcctttgtc tgatttcttg aatttcctaa aagaataaac cgatgcgggt attataattg
     6001 caaggcttgc caaaaaccac gggtttttaa acaaattgct tatccaaaga agaattcttg
     6061 taggcaacgg cagttgaccg ccgatatttt tgaacatggc cacaaaattc ggcacaataa
     6121 acgtcatcat aaacgcgatg accacaattg caattacact taaaaccttg ggatacacca
     6181 tagcggtttt aattttctgc ttaacgcggt tttccttctc aatatatact gaaagcctgt
     6241 caagaaccgt atcaaggcta ccgctgattt ctccgatttc tatcatgctg attacaatgg
     6301 caggaaatgc atctttgtct tgcttcattg aatcggacaa agacactcct ttctgaacat
     6361 cttcatacat ccttgtaagg atttttgcca aatgagcatt ttccgtttgc tttctcaaca
     6421 gatccaggct tccaataata gtaacccctg aatttatcat ggtataaaac tgcttgcaga
     6481 aaagagagat gtcaccgaga gcaattgccc ggtttttttt cagctttatc tccctgtcaa
     6541 ggacacttac ttcactaatt tccataggta aaagaccgcg gcttctcaac aggtctacta
     6601 ccgtatccct atcaactgca tcaagctttc cggccgcagt ttcccctgat gccgtttttg
     6661 ccttatagct gtaaacagcc acatctttcc cctccctgca gtttcaataa cggtcacgat
     6721 aacaaagtat caacttccca gcaatctttt aacgttttcg taagaagagg cataagctaa
     6781 agcatcctca taccttatca tacccttctt atacaggtcc acaagacttg aatccattgt
     6841 tatcattccg aatttggcac cggtttgaat caaagaatcg atttgatgtg tcttgccctc
     6901 ccgtataaga ttctttactg caggtgttgc aatcattact tctacagctg caacacgtcc
     6961 cggaacatcc tgtcttctaa gcaactgctg cgatattact ccctggagcg taaccgataa
     7021 ctggactctc acctgctgtt gtttataagg ctcaaaaacg tcaataattc tgtcaattgt
     7081 acttgccgca ccgacggtat gaagagtgga taaaacaaga tgtcctgttt cagctgccgt
     7141 tatggcaatg gatattgtgt caatatccct catctcaccc accagtataa catcaggatc
     7201 ctctctcagt gcagctttta aagcttttgc ataagataca gcatcatttc ctatttccct
     7261 ctggttcact attgattttt tatgcctatg caagtattca atcggatctt ctaacgtaag
     7321 tatgtggcac tcacgttctt catttatttg atctatcata gaggctaaag tggtggattt
     7381 ccccgatccc gtcggacctg taaccagaat aagccctttg gtttttctgg aaagctccga
     7441 tacaatgtcg ggaagtccca actcctttat tgaaatgact gacgaattca caagcctgat
     7501 tgcagctccg aaagcacctc tctgcttaaa ggcatttacc ctgaatcgcc caatccccga
     7561 cagtgaaaaa gaaaaatcca cctggccgct tttcagcaat tctttcatct gtgcctcatt
     7621 cacaatctgc ttgacaaatt cttcagtatt atcaaatgtc aaagcctcct caccaagctt
     7681 aaccagcttt ccattctttc ttatcgtcgg cggtgctcca acggttatat gaagatctga
     7741 ccccccttgt tccgatactg aacggaataa ctctttagca ttcatatgtt ttcctcctaa
     7801 acgagagtat taagtgttgg aatatgatac ccttaccatc tcttccactg tcgtaatgcc
     7861 tttcaaaaca agtattttgg cgttttcctt gagagtcttc atcccgtttg caatggaaca
     7921 ctctcttaat tcatcttcca aacagcgtct gttaatcaat tctttgtgtt ttttaccgat
     7981 ggacatcacc tcataaacac cgattcttcc tctatatcct gtaccgttac acatagcaca
     8041 accgctgccg taataaattt ttacgggttc cttgagccca agcaaggaga ctatatcatt
     8101 atcggcaacc cgttcccgct tacagttggg gcagattttt cgtacaagcc tctgtgcaat
     8161 aactccgata agcgaagatg ccaccaaaaa cggctctatg cccatgtcaa tcaaccgcag
     8221 caccgtactg ggagcatcat ttgtgtgcaa tgtgctgagc accagatgtc ccgtgattgc
     8281 agaccttact gcaatctgtg cagtttcaag gtctctgatt tcaccaatca ttatgacgtt
     8341 aggatcttgc cttaaaacag accttaaact gttggcaaag tttaagccag tcttatagtt
     8401 aatttgagaa tggtttactc cttcaataat gcattccaca gggtcctcaa tggttataat
     8461 attaatatca ggcttgttaa tctcatttaa agcagtatac aaagttgtag tttttccgct
     8521 tcctgtaggt cctgtaacca gtatcattcc gtaagggttt tttagaatgc cgtcaaacat
     8581 taccatgtct tcatcggtaa gcccaagtct ttctttttgg agaataaaag cgtttttgtc
     8641 agaaattcga ataactattt tttctccaaa tacaatggga aaaaccgata ctcgaagatc
     8701 atattctttg ccgtcaacct ttacggtagc tcttccgtcc tgcggatacc ttttttccgt
     8761 tatatccata ccggaaataa tcttaactct tgtagcgagt gtattcatga tagccgtttc
     8821 gattcgcatt acttcatgga gctggccgtc ggttctgaat cttactttta tttcattttc
     8881 cgacggttct atgtgtatat cgctggccct gtttctaacc gcctgttcaa tcagggaatt
     8941 tacgagctta accgccggtg cgttatttaa atcttcaccc atcctatccg ttttccccgg
     9001 ttcaattttt gacaaggact cacttttctt tttaaattct tcaactgcct tcagggcatg
     9061 ctgattgcca aaatatctgt ctatggcacg ggttatgtcc tcagacgagg caataaccgg
     9121 ttgaacttcc aatcccgtat atatctcaat atcatcgatt gctaacaaat ttaatgggtc
     9181 actcatggca acagtgagca caccgtcatg ctgagctatg gcgataagct cataccgtct
     9241 tgcaaggttt tccgggatca acaaacacgc tgtctggtca atactatatt tctgcagcaa
     9301 aacatgggga atacccagtt gaaactccaa tacctgaatg atgtcttcct gtgtaacaat
     9361 gccctcagat attaacaact cacctagttt tttccccgta gttttctgaa gcctaaggga
     9421 gttgtcgagt tgttcctttg taataagtcc ggattctatt aataggtcac cgagtttctt
     9481 ccttatatgt ttttccataa aaatcacctg tcaatgccga aaaataccac ctcttagtat
     9541 acttgcgaat aaagatttac cgtccctgca agaatctata atacaattgt tcattcttta
     9601 aaaataatat cggcaaaaaa acataatctg ttaacagata aaaaatacta aagaacttca
     9661 attccgattt catgatacga attttaaagg aattgctatg cccttaagat ttttttaaaa
     9721 gaatcatata taaaactaag gtcttcctct gaaaatttca cacctgtcca tatttcatat
     9781 gcactaattc cttgatagaa aagcattccc agtccgttta tcaccttggc acccgctttt
     9841 tccgcatcca taagaaactt cgtcttgacg gggttgtaaa tcacatcata tactatctga
     9901 ttcttgttaa aacatttttt tgcctttatc ggtgtattac cgacttcggg atacatccct
     9961 gcggaagtgg tgttaataat aatatcactt tcctcaaaag ccatcataaa tgttttatcg
    10021 ttaaagttat aaacctgtac aacctccctg atgttttcat ttaccacgtc tgcaagctca
    10081 acagccttca aagttgtacg gttcacaaga cttatttttt ctgccccttc ctttgccaat
    10141 ttaacggcaa ttgaacgggc tacacctccg gctcctataa gcacaacctt tttcccgccg
    10201 aggcccacac cggcttcctc cctaaaggac cttgcaaatc cttcggcatc ggtgttgtag
    10261 ccaaaaagcc gtccgtctat gttttttacc gtatttacgg ctcccatcag tatggcatct
    10321 ttggtgttct catcgatata tttcattatt tctcttttgt acggaacagt tacgttaaaa
    10381 ccgataaact ccagacgttt taatgcgttt acaactgtcc cgatatcctg tttttcaact
    10441 ttaagcggta catatacaat gtcatgacct aaaatagagc tcaatgtatt atgaagctgc
    10501 ggtgatatgg aatgctcaac cggattgccc ataagcccta ataattttgt cttacccgtt
    10561 accctaacat cgatctccat aataattcct cttctccatc tattttacct gaaacgaaga
    10621 ctcttgcccc tgttgagatt gagtatatca acaatatgtc ttatactata taaaaatctc
    10681 actttctgct tttttgaagg gtcctataat atcttgcaag aacaatgcct tcgaaagccc
    10741 tcctgatcct gacacctata aactctcttc tgtcaattgg ctgtaccagt atggtaaaca
    10801 tattaagtct gtttccccca tatatatcgg taaaaatctg gtctccaatt acggctgttt
    10861 ccgatggctt tgttcccatt agttccatag ccttcaaaaa agattttctt cccggctttg
    10921 aagccctgtg aatagcataa agctttaatc tttcattgaa ttttaccacc cgcttttggg
    10981 atgcattgga tactatacag gccttaaatc cctttttctt aattttctca atccattcca
    11041 atgctttctc atctgcctct tgtgcatgtt ccggcacaag ggtgttgtct atatcaagaa
    11101 cacatccctt gatattgttc ttgattagga aatcgaggtc aatatcctgt atcttgtcta
    11161 cttttaaatc cggataaaat ttttctagca tttttacctc caggttattt tcatatcttt
    11221 cttatagaac tataatagca aataatgtat cggtatatca attaataaat gatatttgat
    11281 attattattt attttatcct gccatatgca aattcttcgt gctataaagc atgcattgaa
    11341 caaaatcaaa taaaaaatat ggaaataaat gagtttcgca agtacaggac atgtataaag
    11401 attaaatatt ttagctaaaa gtactggatt tttaaataaa aaagtagcat aatagataat
    11461 aagattttgt agaattggat tttttgcatt caacagaccg taaatcgaag ggatttgatt
    11521 cccatgagat tttaattccg aataccctct acaaagtatg atatcggaat cataaatata
    11581 acactatttt attttgaaag gggttaaaat tatgtcatac cagataagta ttcaaaaaac
    11641 acaaa
//
LOCUS       DAOOJC010000013        11614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_5089_length_11614_cov_3.846440, whole genome shotgun sequence.
ACCESSION   DAOOJC010000013 DAOOJC010000000
VERSION     DAOOJC010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 11614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11614
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5089_length_11614_cov_3.846440"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>5173
                     /locus_tag="PLA01_00725"
     CDS             <1..>5173
                     /locus_tag="PLA01_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPC99855.1"
                     /translation="NAVAYAAAEGLIQGYENGTFKPRDNMSRQDAAVLVAKLFDVSFF
                     EGAQDYKFTDEDTFPEYSYKSIKNLASHEIVQGYPDGTFRPTRLITRAEAVRMLDVVL
                     KYIEVPEETIPVAPPETATPTYTPAATPTAIPTSTPDTGSNKPDPGPVSTPKATAAPT
                     EIPRIHKIYTDDEDFNEGTKINLINKVSGQLKLDDTTNPFNFIWVAVSTKGTVVKIDT
                     DTGKVVGEYRTAPQGQPTNPSRTTVDHNGNVWVANRDGNSVVRIGLAENGQWIDKNGN
                     GVCDTSTGLGDIRPWSNAGGADTNGGVSTAEDECIINYVKVSSSGTRHVSVDSNNDVW
                     VSGTGNRIFNLIDGETGEIKRTEGPVGYGGYGGLIDKNGVIWSARPFLRWDTSLPLTG
                     PKGGNWLGLDHDSYGLAIDSQGNVWNASHTENQIRKFAPDGTLIGTYQHGSYYAQGCV
                     VDKNDDVWVAHYYGGDKTIGHIKNDGTFVGNVEIPSGATGVAVDAKGNIWAICYNTRN
                     VVRINPNAGPIGEDGKTPVGEVDFVSEDLGGNLYNYSDMTGSTLTGAPKVGTWSTVFD
                     SKQEGTEWGVIDWNGRVYNDGKIEVYVSSSEDNVTFSSPVQVDNGSSFELPAGQYIKI
                     EVRFTRSTDGKSPELYDLTIGEKLYKIKSVKNGAPRVEAGSNKTMNEGDTITLSGDVW
                     DDLRPLGKPLTFLWEKVSGPGEVSFSSEQSYNSEVTIDKPGNYVLRLSSSDYQSTGRD
                     ELKIEVKALPTPTPTQAPTPSPTPSPTPSPTPSPTPSPTPDPTPTPEPTPESTLTPTQ
                     SPTPSPTPTLSTLKVNIGIVEGKINEEIIIPVFFEGASKSGIHSCEFILSFDSDVIRV
                     QEVIAGNIIENSDANFGTIINNEAGTIKFVFSDEMESDLISVDGEFANIKATINETEK
                     IGFSPIELVEENFIDANLNTVIVTFNGGGVNVKEIEITPTPEPTLEPTAEPTPEPTQE
                     PTQEPTPEPTSEESDFIRPVVDISINPKIAKVGQAVSISVMASDDNRLESKTLLVNNE
                     IVEIDEQGSAKYVSDNPGTFKVVAKAYDAQGNEGYKSDEIMFLESGDSKPPAVSISSP
                     VEDTTLYMPVDIIGTAMDDNLLMYKLEYSIKDKGEFIEFARGTSSVNNGVLGKLDTTS
                     LHNGIYDIKLTAVDKSGNGVSITSSFQVAGEAKIGNFTVSFEDMMAYVAGIPINIVRT
                     YDSRNKYKGDFGTGWTLAVKSMQVSESCEPASNWSYRAISGGLFTNYYLTESKPHTVT
                     ITYPNGKTDEFRTVINPESSIIPFDGRPVAISYKPKAGTNSKLEALYDDTALVLTEGE
                     NKAVLYTYDLTEKYNPDRYKLTAEDGTIYIINQHTGLESITYTDGSSITFSNKGIIHS
                     SGKGIVFKRDSEGRITSVTDPKGYSVSYEYDYYGDLISVTDQEGNVTRYKYNSNHGLV
                     DMLDPRGRKPVRNEYDDSGKLTAHIDAEGNRIEFSHDIDARQEIVKDKYGYITVFYYD
                     ETGNIIEKVDALGNKTSYEYDANNNMVKETNPLGKTTSYTYDSNNRMTSKTDPMGNKY
                     EYKYNERGQIEKVIEPEGNTTVYEYDNNGNLVTVTDPMGYKKVYNYDSEDNMTGYTDE
                     MNNTTTYTYKNSFMMTETSPLGVVTYYDYDDNGNMISKLIKRTTESGIEDIETKYVYD
                     GKNRLIEEIDAYGNSTKMGYNEIGEVAFEADKNGRYTYYEYDILGNLAEVKYPDGT"
     assembly_gap    5174..5273
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5274..7828
                     /locus_tag="PLA01_00730"
     CDS             <5274..7828
                     /locus_tag="PLA01_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HPC99856.1"
                     /translation="YEYDILGNLAEVKYPDGTKETYTYDKGSRKTSFTDRDGRTTKYD
                     YDKIGRLVKTIYADGTSEEIKYDAAGRITKKINERGFETEYKYDDAGRNTEVIDALGN
                     VTLYEYDKNGSVIKMTDAKKNVIEYTYDLCKRKTQTIYNDGTSISIEYDKVGRKIAET
                     DQAGKTTEFKYNEDGKIVKVIDALKNTTEFGYDEAGNRITETDANKNSITYEYDKLGR
                     KTKRILPMGMSESFVYNSQDKVSEYTSFKGDVLKYEYDSNGRLIKKVFPDKTTEEYAY
                     TGTGQLISVKDSRGVTSFEYDLRDRLVKQVNPDGTLIEYEYDDAGNRTAVKTSSTTVT
                     YSYDKLNRLSTVTDSKGNVTNYEYDAVGNRIGIKYPNSTETEYVYDDLNRLVKLINRK
                     SAGEIISSYEYELGLAGNRVKVTENTGRTVEYDYDDTYKLLSETVNEPGEEEKITVYE
                     YDQVGNRLSKNADGVVIEYSYDKNYRLLSDGTYTYTYDDNGNTLSKEKEDLSVKYDYD
                     YQNRLTKVTTTTETGSSEISYIYDFGGICVEKTVDGEVSKYIVDKNREYSQVIEETDA
                     AGTTIVKYTVGDDLISQERNGVVSYYHYDGLGSTRILTGEDGNITDQYTYDAFGNIIS
                     SSGNTVNEFLFTGEQIDLNTGFYNLRARWYNPEIGRFMTMDTHPGSIFDPRTLHKYLY
                     CGNDPVNNTDPSGEYFLISITIGTSISGMLRSYALTNLKILFDAIYMAQTMLKPALEI
                     QNYCYNAILSGYADSNIDSLFRSAKELECEAYRFLAQSIVNNYKTFAKGLIFTVKFKL
                     VLNPETEQALDLKKLEKYMNLLIGAVNGNEKDKAELYKNTVCDTVNTLLSKM"
     gene            8244..8627
                     /locus_tag="PLA01_00735"
     CDS             8244..8627
                     /locus_tag="PLA01_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99857.1"
                     /translation="MVEYMEEKYGEEFTFIEATGTNKIKGSSQREATVSTERFPREQI
                     SVMADKDEDGNYYFRDNYVSFLLRDEIEKLLTEIAAPIYKDCKVIFDANTPVYPEETG
                     KEITMDEYIKWGSLNIYIYMFRSGC"
     gene            8629..8865
                     /locus_tag="PLA01_00740"
     CDS             8629..8865
                     /locus_tag="PLA01_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99858.1"
                     /translation="MEKKDEDIEKLRLKLKEKGVSCSILLWYFKDDKLGGIDHSNYRR
                     YRNYSDSEIREELKTIGVFDMHPSTYEFYYSNWW"
     gene            9163..9372
                     /locus_tag="PLA01_00745"
     CDS             9163..9372
                     /locus_tag="PLA01_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99859.1"
                     /translation="MKKNLKSQHKKTTHLIANQKGFGIKEIAISLGAVVAVGIILTVF
                     KDQAGTILNDVWNMIKDFIGNNIKS"
     gene            9426..9875
                     /locus_tag="PLA01_00750"
     CDS             9426..9875
                     /locus_tag="PLA01_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99860.1"
                     /translation="MKGVANSILAVILISFFILSFTNFLLFFPWYLTLVYNTFNTATE
                     ASIVNYVTGDMVGNVINDLESKPLYKDYIGDVKVYMNDRVIKEGENDTNLRLQKGESF
                     NVSIEAQFPFVVKVFGQDFERKLPIKFTIPTTGVRYYKDMDPNAYIN"
     gene            9886..10332
                     /locus_tag="PLA01_00755"
     CDS             9886..10332
                     /locus_tag="PLA01_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99861.1"
                     /translation="MKFVAYILAAVIFFTVLLAPFVELLSAFGDMCKISAAVSASSRA
                     AIMSSEVVESAMDAEAKIDFDKFKSIFKDNFCSALNLNEYYRSNDDKYNDFTVKFELL
                     EDDKCKIEVKTVYKFKTALFNKYLSELSSDSNKLRVVKTQTLEIEN"
     gene            10407..11222
                     /locus_tag="PLA01_00760"
     CDS             10407..11222
                     /locus_tag="PLA01_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HPC99862.1"
                     /translation="MIKVEADAYTHKGLIRDKNEDNFFLDGIFIDENESNNCARFSIS
                     KSSKEYIFAVCDGMGGHKYGEKASFAAVKILKKLHEKIAENSGRKDIEAIIRCLKSYV
                     LKANEYIYDMSVKKGSPVGTTFACLTISDNKAVALNLGDSRVYLIRKGEIKRLTTDHT
                     EAERLIRLGALTRESALTHNSRHRLYRYFGISPSEGILEPDFSDIIEIEKDDKFLICS
                     DGLTDTIEEKLLLEIARRKMKNSEMCASFVDEALNRGGSDNITSVLINIKDVG"
     gene            11252..>11614
                     /locus_tag="PLA01_00765"
     CDS             11252..>11614
                     /locus_tag="PLA01_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HPC99863.1"
                     /translation="MQSDIDYIKRYEPLWGCWYVENLIGEGSFGQVFKIVKDEWGFRY
                     EAALKVIRVPTKGQYREASAVMATNDNSLMDYFKDSVKNIVNEIRVMYTLKGNSNIVG
                     YEDHMVIKRDDPPGWDILL"
BASE COUNT         4020 a         1750 c         2638 g         3106 t
ORIGIN      
        1 ataatgccgt ggcttatgct gctgctgaag gcttaataca gggatatgaa aacggaacgt
       61 tcaagccaag ggataatatg agcaggcagg atgcagcggt attggttgca aaactgtttg
      121 atgtcagttt ttttgaaggg gcacaagatt ataagtttac cgatgaggat acatttcctg
      181 aatattcata taaaagcata aaaaatcttg catcccatga aatagtacag ggataccctg
      241 acggaacatt caggcccaca aggctcataa ccagggccga agcggtcagg atgctcgatg
      301 tagtgttaaa gtatattgaa gttccggagg aaactattcc ggtggcacca cctgaaacgg
      361 cgactcctac atatacgcct gcagcgacgc cgacagcaat accgacctca acacccgata
      421 cagggagcaa taaaccggac cccggaccgg tttcgacacc aaaggcaacg gctgcaccta
      481 cggagatacc taggattcat aaaatttata ctgatgatga agattttaat gaaggaacca
      541 aaataaatct gataaataaa gtgtcgggcc agcttaagct cgatgatact acaaacccgt
      601 tcaactttat ttgggtagcg gtttcaacaa aaggtactgt agttaagatt gatacggata
      661 cgggcaaagt tgttggggaa taccgtacag ctccgcaagg acagcctaca aacccatccc
      721 gtactacagt agaccacaac ggaaatgtat gggtggcgaa ccgtgacggt aatagcgtag
      781 taaggatagg acttgcagag aacgggcagt ggattgacaa gaatggaaac ggagtatgtg
      841 atacctcaac aggcttgggt gatataaggc cgtggagcaa tgccggcggt gccgatacaa
      901 acggaggagt ttcaactgct gaggatgagt gcataataaa ctatgtaaag gtaagttcat
      961 cgggaaccag gcatgtatct gtagattcca acaatgatgt atgggtgagc ggaaccggaa
     1021 accgcatatt taatttgatt gacggcgaaa ccggtgaaat aaaaagaacg gaaggccctg
     1081 taggatatgg cggatatggc ggactgatag ataaaaacgg tgttatatgg tcggcaagac
     1141 cgttcttaag gtgggataca tcactgccat taacaggacc taaaggcggc aattggttag
     1201 gtcttgacca tgacagttac ggtttggcaa ttgacagcca ggggaacgtt tggaacgcat
     1261 cccatacgga aaatcaaatc agaaaatttg ctcccgacgg aacattaata gggacctatc
     1321 aacacgggtc ttattatgcc cagggatgtg ttgtggataa aaacgatgat gtatgggtag
     1381 cccattatta tggcggagac aaaaccatag gtcatattaa gaatgacgga acctttgtcg
     1441 ggaatgtgga gattccttca ggggctacag gagtagcagt ggatgcaaag gggaatatat
     1501 gggcaatatg ttataatacc agaaacgtag taaggataaa tcccaatgcc ggaccgatag
     1561 gtgaggacgg caaaactccc gttggagaag tagactttgt ttctgaggat ttgggaggca
     1621 atttatacaa ttacagtgat atgaccggaa gtacccttac cggggcaccg aaggtgggaa
     1681 cctggtctac ggttttcgac agtaagcagg aaggaaccga atggggtgtt attgactgga
     1741 acggaagagt gtacaatgac gggaaaattg aggtatatgt ttcaagcagt gaagataacg
     1801 taaccttcag tagtcctgtt caggtagaca atggtagcag ctttgaatta ccggcaggac
     1861 aatacataaa aatcgaagta aggtttaccc gttcaacgga cggaaagagt ccggaattat
     1921 atgatttaac cattggggaa aagctttata agattaagtc ggttaaaaat ggggcacctc
     1981 gagttgaggc aggcagcaat aagactatga acgagggtga tactataacc ctatccggag
     2041 atgtctggga cgatttaaga cctttgggca aaccattgac atttctttgg gagaaggtat
     2101 ccggtccggg tgaggtatcc tttagttcgg aacagtcgta taattcagaa gtgacaatag
     2161 ataagccggg gaattatgta cttcgccttt catcaagcga ttaccaatcc accggaaggg
     2221 atgagttgaa gatagaggtt aaggcgttgc cgacacctac gccaacgcag gcaccgacgc
     2281 catcgccgac gccatcgccg acaccatcac caacaccatc accgacgcca tcaccgacac
     2341 cggacccaac acctacaccg gaaccgaccc cggagtcgac gcttacgcca acgcagtcgc
     2401 cgacaccatc accgacacct acactgagta ctcttaaggt gaatataggg attgttgaag
     2461 gtaaaataaa tgaagagatt attattcctg tattttttga aggtgcttca aaaagcggga
     2521 tacatagctg tgaatttata ttgtcctttg actctgatgt tataagagta caagaggtta
     2581 ttgcagggaa tataattgaa aattctgatg caaatttcgg tacaataatt aataatgaag
     2641 caggaactat aaagtttgta ttctcggatg aaatggaatc ggatcttata agcgtagacg
     2701 gtgaatttgc aaatataaag gcaacgataa atgagactga aaaaatagga ttcagcccga
     2761 tagaattggt tgaagaaaac tttatagatg ccaacttaaa tacagtaatt gttacattta
     2821 acggtggcgg tgtaaatgta aaagaaatag aaataacccc gacaccggaa ccgacgctgg
     2881 aacctacagc agagccaacg ccggaaccga cacaagaacc cacacaagaa ccgacaccgg
     2941 aaccgacatc agaggaaagt gactttattc gtcctgttgt tgatatttct ataaatccca
     3001 aaatagcaaa agtagggcag gcagtatcaa tatcagtaat ggcatcggat gataacagat
     3061 tggaatcaaa gactcttttg gtaaataacg aaatagtaga aatagatgag cagggtagtg
     3121 ccaaatatgt gtcagataat cccggaactt ttaaagttgt tgcaaaagcc tatgatgcac
     3181 aaggaaatga aggatataaa agtgatgaaa tcatgttttt ggaatcggga gattcaaaac
     3241 ctcctgctgt tagtataagt tcgcctgttg aggacacaac actttatatg cctgtagaca
     3301 ttatagggac tgcaatggat gataatctac taatgtataa gttggagtat tcaattaagg
     3361 ataaaggaga atttattgaa ttcgccaggg gaacttcttc agttaataac ggagttctcg
     3421 gtaaactgga cactacatca ttgcataacg gaatatatga tattaaactt acagcagtgg
     3481 acaagagcgg aaatggagta tctattacca gtagttttca ggtagcgggg gaagcaaaaa
     3541 taggtaattt cactgtcagc ttcgaggata tgatggcata cgtagcggga attcctatta
     3601 atattgtccg tacttatgac agccgaaata aatacaaggg tgatttcggg acaggatgga
     3661 cgttggcagt aaagagcatg caggtaagcg aaagctgtga gccggcttca aactggagct
     3721 atagagcaat aagtggtggt ttattcacta actattactt aacggaaagc aaaccgcata
     3781 ctgtgactat tacatatccg aatggaaaga cagatgaatt tagaactgta ataaatcctg
     3841 aaagcagcat aattcctttt gatggcagac ctgttgcgat ttcttacaaa ccaaaggcag
     3901 gaacgaattc gaagcttgag gcattgtatg atgatacagc tttagttttg actgaaggag
     3961 aaaataaagc tgttttgtat acatatgact taaccgaaaa atataatcca gacaggtata
     4021 aattaacagc agaggatgga actatatata ttataaatca gcacacgggg ctggagagca
     4081 ttacttatac agatggaagc agcattacct ttagtaataa gggaattata cattcttcag
     4141 gaaaaggtat tgtatttaag agggattcag aaggacgaat tacttccgtt acggatccta
     4201 agggttatag tgtatcgtat gaatatgatt attatgggga tcttataagt gtcacagatc
     4261 aggagggaaa tgtaaccaga tacaaatata attcaaatca tggccttgtt gacatgcttg
     4321 accctagagg cagaaaacct gtgcgaaacg aatacgatga cagcggtaag cttacagcac
     4381 atattgatgc tgaagggaac agaattgaat tttctcatga tattgatgcc cgtcaggaaa
     4441 ttgtaaaaga caagtatggg tatattaccg tattttatta tgacgagacg ggtaatatta
     4501 ttgaaaaggt tgatgcgtta ggaaataaaa ccagttatga atatgacgca aataacaata
     4561 tggttaagga aacaaatccc cttggaaaaa ccacatctta tacttatgac tccaataacc
     4621 gtatgacaag caagacggat ccaatgggaa ataagtatga atataaatat aacgaacggg
     4681 gacagataga aaaagtaatt gaacctgaag gaaacacaac agtatatgaa tatgataata
     4741 atggaaatct tgttactgtt acggacccaa tggggtataa gaaagtttac aattatgatt
     4801 cggaagataa catgaccggt tataccgatg aaatgaataa tacaacaacc tatacctata
     4861 aaaacagctt tatgatgacg gaaaccagtc cattgggggt agtcacttat tatgattatg
     4921 atgataatgg caatatgata tctaaattga tcaaaagaac gacagaatca ggaattgagg
     4981 atattgaaac caagtatgtt tatgatggga aaaacaggtt gatcgaagaa atcgatgcat
     5041 atggaaacag tacgaagatg ggctacaatg aaattggaga agtagcattc gaagcagata
     5101 aaaacggcag atatacatat tatgagtatg atattctcgg aaatcttgct gaagtaaaat
     5161 atcctgacgg aacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnattatga
     5281 gtatgatatt ctcggaaatc ttgctgaagt aaaatatcct gacggaacaa aagagaccta
     5341 tacttatgac aagggaagca gaaagacatc tttcacagac cgggacggac gtaccaccaa
     5401 atatgactat gataaaatcg gacgtcttgt taaaacaatt tatgctgacg gtacctctga
     5461 ggaaataaaa tatgacgctg caggaaggat aaccaaaaag ataaacgaga gaggatttga
     5521 aactgaatac aaatatgatg atgcaggaag aaatactgag gtaattgatg cattaggaaa
     5581 tgttactcta tatgaatatg acaaaaacgg cagtgtaatt aaaatgaccg atgcaaagaa
     5641 aaatgttatt gaatacacgt atgatctttg taagagaaag actcagacaa tttataatga
     5701 tggtacgtca atatctatag agtatgacaa ggtcggcaga aaaatagccg agactgatca
     5761 ggccggaaag acgacggaat ttaagtataa tgaagacgga aagattgtaa aagttattga
     5821 cgccttaaaa aatactacgg aatttggcta tgacgaagcc ggcaacagaa ttaccgagac
     5881 ggatgcaaat aaaaacagta ttacatatga atatgataaa ctgggcagaa aaacgaagag
     5941 aattctaccc atgggaatga gcgagagctt tgtatataac agtcaggata aagtttcaga
     6001 gtatacaagc ttcaaaggtg atgttttaaa atatgaatat gattccaacg gaagactgat
     6061 taaaaaagta ttccccgata agacaacgga agaatacgca tataccggca ccggtcagct
     6121 gatatccgtt aaggactcaa gaggtgtaac ttcctttgag tacgatttga gagacaggtt
     6181 ggttaaacag gttaacccgg acggtacttt gattgaatat gaatatgatg atgcaggaaa
     6241 cagaacggct gttaagacct caagcacaac cgtaacatat tcttatgata aattgaacag
     6301 gctgtcgact gttaccgatt caaaaggaaa tgtaacaaac tacgaatatg atgcggtggg
     6361 caacagaata ggcattaagt atcctaattc tactgagacc gaatatgttt atgatgactt
     6421 gaaccggctt gtaaaactaa taaacaggaa gtccgccggt gagattattt cttcctatga
     6481 gtatgaactc ggactcgccg gtaacagggt taaggttact gaaaatacag gcagaaccgt
     6541 cgagtatgat tatgatgaca catataagct tttgagcgaa actgttaatg agcccggaga
     6601 agaggaaaag ataactgttt atgagtacga ccaagtaggc aacaggttat ctaagaacgc
     6661 agacggagtt gttatcgaat attcctatga taagaattac aggttgctgt ctgacggtac
     6721 atatacttat acatatgatg ataacggcaa cactttgagc aaggagaaag aagatctgtc
     6781 ggttaaatat gactatgatt accagaacag attgacgaag gttacaacaa caacggaaac
     6841 cggctcttcc gaaataagtt atatatacga ctttggcgga atttgtgtgg aaaagaccgt
     6901 tgacggtgaa gtaagcaaat atattgtgga taagaacaga gaatattcac aggtaattga
     6961 agaaactgac gctgcaggta caacaattgt aaaatatact gtcggagatg acctgataag
     7021 ccaggagaga aatggagttg taagctacta ccattatgac ggtcttggaa gtacaaggat
     7081 tttaacgggg gaagacggta atatcaccga ccaatatacg tatgacgctt ttggaaacat
     7141 tatttcaagt tcagggaata cggttaatga attcttattc actggagagc aaattgactt
     7201 gaacacagga ttttataatc tgagagcaag atggtataat cctgaaattg gaagatttat
     7261 gactatggat acgcaccccg gatcgatatt tgacccgcgt actttgcaca aatacttata
     7321 ttgcggaaat gatcctgtta acaatactga tccgtcaggg gaatacttct taatttcgat
     7381 tacaatagga acatccattt ccggtatgtt gcgaagttat gcattgacaa atttaaaaat
     7441 tctgtttgat gcgatttata tggcacagac aatgttgaaa ccggccttgg aaatacagaa
     7501 ctattgttat aatgctatac tcagcggata tgccgacagt aatattgata gcctgtttag
     7561 atcggcaaaa gaactcgaat gtgaagctta tagattctta gcacagtcta ttgtcaacaa
     7621 ttacaaaact tttgcaaaag gtttaatttt tacagttaag tttaagctgg ttttaaatcc
     7681 tgaaaccgag caagctctgg acttgaagaa gcttgaaaaa tatatgaatc tgttaattgg
     7741 tgcggttaac ggtaatgaga aggataaagc tgagttatat aaaaatacag tatgtgatac
     7801 tgtcaataca ttacttagca aaatgtagaa aaatgacatt ttgaatatta agtttcagtt
     7861 taactcgttc agcttcggct gaacgagttg gcttttatta gcgggatttt acgaagtgaa
     7921 atgaccgatg gttaaacggg ttttgaggat ttttatttga atggaaatct gagaaactta
     7981 ttccagggac agatacaaaa caattatgta tatgggttga tatgaagcga agcattgaga
     8041 tataatatta ccaggtttta gcaagatttt cagagaaatt gatccaactg atagctgata
     8101 catatttgca gtttaattat ttaaagaaga aaggtgaagg agaggctatg aaaagagtag
     8161 ttataatcgc tttaatttca actatgatga taggactgag tgggtgtatg tttattcaaa
     8221 atggaaattc aatatataaa caaatggtag aatatatgga agagaagtat ggagaggaat
     8281 ttacatttat agaagcgacg ggaacaaata aaataaaagg ttcatcgcaa agggaagcta
     8341 cagtgtcaac ggagaggttt ccgagagaac aaatcagtgt tatggcagat aaggatgagg
     8401 acggcaatta ttattttagg gataactatg tttcgtttct tctgagggat gaaatagaga
     8461 agctgctaac agaaattgct gctccgattt acaaggattg caaggttatt tttgatgcaa
     8521 atactcctgt ttatcctgaa gaaaccggaa aagagataac aatggatgaa tacataaaat
     8581 gggggtctct gaatatatat atatatatgt ttaggtccgg gtgctgatgt ggagaaaaaa
     8641 gatgaagaca ttgaaaaact tagattaaaa ctaaaagaaa aaggggtctc atgcagtatc
     8701 ttactatggt acttcaagga tgataaattg ggaggcatag accatagtaa ttataggaga
     8761 tatagaaatt actccgatag tgaaattcga gaagagctaa agacaattgg agttttcgat
     8821 atgcatcctt caacgtacga attttattat tctaattggt ggtgattttt atggatgaaa
     8881 gaatgccttt atactgtttc ttttttgaag taaaatagga tgcatgtcaa gctcggattt
     8941 tttatatgat atttttttct tgaaatttgt ggtgctaagt tctaaagacg ggtatagaaa
     9001 aatgcagaaa gaacgtaagc tttaaagaaa ctttctttac atttactaca taaattggta
     9061 tgtcgtgtca agtcttatat attgaaattc aatgaataat ttggtattat aaatacaaag
     9121 gtaaatacaa atgtggttgg aaaaatgagg gggaatataa atatgaaaaa aaatttgaag
     9181 tctcaacata aaaagactac gcacttaata gccaatcaaa agggttttgg aattaaagaa
     9241 atagcaatat ctttaggggc agttgttgct gtgggaataa ttcttacggt atttaaggat
     9301 caagcaggta caattttaaa cgatgtctgg aacatgataa aggactttat tggcaataat
     9361 attaagtctt aaacttgctg taatatttag tatttcaatt ttaagttaac ttttggaggt
     9421 ctagcatgaa gggagtagct aattccattc ttgcagttat tttgatttca tttttcatat
     9481 tgtcatttac caatttttta ttatttttcc catggtatct aactttagta tacaatactt
     9541 ttaatactgc tacggaagca tccattgtaa attatgtaac cggagatatg gttgggaatg
     9601 tgataaatga tttggagtca aaacctttat ataaagacta tataggtgac gtaaaggtat
     9661 atatgaatga tcgtgtgata aaggaagggg aaaatgacac gaatcttcgc cttcagaaag
     9721 gagagagctt taatgttagt attgaggctc aatttccttt tgtagtaaaa gtattcggtc
     9781 aagattttga aaggaaattg ccaatcaaat tcaccattcc cacgacaggt gtcagatact
     9841 ataaggatat ggaccctaat gcttatatta attaggagga attgaatgaa atttgtagcc
     9901 tatatattgg cggcagttat tttttttact gtattgttag ctccatttgt ggagcttctt
     9961 agtgcctttg gggatatgtg caaaatcagt gctgctgtta gtgcatccag tcgtgccgcg
    10021 ataatgagtt cggaagttgt tgagagtgcc atggatgctg aggcaaagat tgattttgat
    10081 aagtttaaaa gtatttttaa agataatttt tgttctgcct taaatttaaa tgaatattat
    10141 cgatccaacg atgataagta taatgatttt actgtcaaat ttgagctgtt agaagatgat
    10201 aagtgcaaaa ttgaagtaaa aaccgtatac aagtttaaga ctgctctgtt taacaaatat
    10261 ttatcggagc tttcgagtga tagtaataaa ttaagggtag ttaaaacaca gacattggaa
    10321 atagaaaact aaaatataga aagagtacag cagatataaa attaatccag agtatgtaat
    10381 gatttatata agggttggag gttaatgtga taaaagttga ggctgatgcc tatacacaca
    10441 aaggattgat tagagataaa aacgaagaca atttcttcct ggatggtatt tttattgatg
    10501 aaaacgagtc aaataattgt gctaggtttt caatttcaaa atcttcaaag gaatatatat
    10561 ttgccgtatg cgacggtatg ggcgggcata agtatggaga aaaagcatct tttgccgctg
    10621 tgaaaatatt aaaaaagctt catgaaaaga ttgcagaaaa ttccggcagg aaagacattg
    10681 aggctattat tcgttgcctt aaaagttatg tactaaaggc aaatgaatat atatacgata
    10741 tgtccgttaa aaaaggcagt cctgtcggaa ctacctttgc ctgcctgacc atctctgata
    10801 ataaggcagt ggcgttgaat ttgggtgata gcagagttta tttgatccga aaaggagaaa
    10861 ttaaaaggct caccactgac catacagaag ccgagcggct tataagactc ggagccttga
    10921 cccgggaatc ggctttaact cataatagca gacaccgtct atacagatat tttggaattt
    10981 ctccttccga aggaatattg gaaccggatt tttcggacat tattgaaata gaaaaggatg
    11041 acaaattttt aatatgcagt gacgggctta cagacacaat tgaagaaaag ttattgctgg
    11101 aaatagcccg gagaaaaatg aaaaacagtg aaatgtgtgc atcttttgtg gatgaagctt
    11161 taaacagagg cggcagtgat aatattactt ctgtattaat taatattaaa gatgttggtt
    11221 agttaaaggt gtagttagta ggaggtaagc tgtgcaaagc gatatagatt atataaaaag
    11281 atatgaaccc ctttggggtt gctggtatgt tgagaatttg attggagagg gaagctttgg
    11341 gcaggtattt aaaatagtta aggatgaatg gggatttagg tacgaagcgg cattaaaagt
    11401 aatacgtgtg cctacgaagg gacaatatcg tgaagcttct gcagttatgg ccacaaatga
    11461 taattctctt atggattatt ttaaggattc cgttaaaaat attgttaatg aaataagagt
    11521 tatgtataca ctgaagggta acagcaatat tgtaggatat gaagaccaca tggttataaa
    11581 aagggatgat ccgccgggtt gggatattct tctt
//
LOCUS       DAOOJC010000014        11352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_5213_length_11352_cov_4.096663, whole genome shotgun sequence.
ACCESSION   DAOOJC010000014 DAOOJC010000000
VERSION     DAOOJC010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 11352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11352
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5213_length_11352_cov_4.096663"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="PLA01_00770"
     CDS             <1..1003
                     /locus_tag="PLA01_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99864.1"
                     /translation="KFFKIDVKVNKKLDDINNVDINLKYVKGYYAQSYAGTGEHTDSD
                     GDIPDGEVSKAVISDPTGIAVSGDRIYVADRRLRVIENGEINTMPMDMDVGTVRTDLN
                     GNVYFSLLPWLDEDEQMLTGIFKLDNEGANSVYVIDEPFSNIGDFAFDSQGNMYVLIH
                     QEILNDMSEQTKIVYVDIQTQNAVVMREIGGIIDSITVDGDDNVYISSSVNGAIYKWE
                     KGEEEFKYFAGKENDRHFIDGINNRFFAPVKIAAREDFLYVIDQNVIRRIALKDGKLV
                     DVETIAGQAGDGEKNLDKQEGVDAWFDRPVDITFDDQGNIYVSDKDAYIIWKILNEE"
     gene            1118..2191
                     /locus_tag="PLA01_00775"
     CDS             1118..2191
                     /locus_tag="PLA01_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99865.1"
                     /translation="MIRIKLVILERDENYITRITNAINANYSDKLELFAFTKQDTFID
                     FIKNNKVDIILASDTFDVNLKEIPERIQFAYFVDSPSLESLNNKKAICKFSKISLIYK
                     EILNLYSENPVNVFNLKKDGSGETGILTFLPCNGGDGASVMAAACCLNFADRGRRVLY
                     LNLESFGTPDIFFDAEGTFNLSDLIYSIKSRKSNIALKIESMVKKDKSGVFFFDECKV
                     PLDLMEMTGEDIQILFDVINESNLFDYVVVDTEMALNDVILQIMQNSNRLIYVTDGSQ
                     TSNLKFKRIYSTLEIVEGQRNLSLLPKISIAYNKTNCISNKYIEEIQNINVLGWAGEC
                     IKASSAQAAHQISKMSMFDTLLI"
     gene            2204..3442
                     /locus_tag="PLA01_00780"
     CDS             2204..3442
                     /locus_tag="PLA01_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008751985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HPC99866.1"
                     /translation="MDENKLHEIVISIRDHIYANLSLSELGDEELRQEIENIVLQRTS
                     DTYLSIEDKISIIEQVFSAIRGLGVLDSIIDDESITEVMINGPDNIFIERKGKVAKLD
                     QKFESGRKLEDVIQKIVGSVGREVNQANPIVDARLKDGSRVNVVLPPISLKGPVVTIR
                     RFSKTPMTIEKLIEFGSITEEIAEKLELLVRAKYNIFISGGTGSGKTTFLNALSNFIP
                     KDERIITIEDSAELQIKGVDNLVSLETRNANVTGSGRISIRDLIKASLRMRPDRIVVG
                     EVRGEEALDMLQAMNTGHDGSLSTGHANSTRDMLSRLETMVLSGSSGLPLDAVRQQIA
                     SAIDVMIHLARLRDRSRKTMEITEIVGYENGKIILNPLYVFEEDENTTLEKVSGKLKR
                     TNNPMVNVDKLRLSGINTVI"
     gene            3453..4253
                     /locus_tag="PLA01_00785"
     CDS             3453..4253
                     /locus_tag="PLA01_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99867.1"
                     /translation="MEYTDYSVYKMKASEKIMYFLLAFAVGAFVGYVFYGGIGKDADG
                     NPTTLTHILNIIISGTTGIIAGRFFIPIMTKQIIKKRKKSLHLQFRDMLESITASLNA
                     GRNVPDSFLSAYDDLKVQYPEGAFILKELEIILSGLENNVNIEKLLLDLGKRSGNNDI
                     MSFASVFEICYRKGGNIKDVIKDTHQIISDKIEIEMDIETMVTSNKIEQYIMTIMPIA
                     LVGVVKIMSPDFGDKFVSPMGIISTTIAVGIFVVAYFIGKEILSIKGV"
     gene            4265..5137
                     /locus_tag="PLA01_00790"
     CDS             4265..5137
                     /locus_tag="PLA01_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPC99868.1"
                     /translation="MFISLEVVVLLGIGTFFSLIWAVLLLTSGKYNELVAPLDKKEFP
                     LKELYPLGYKILDIIKYGYNTKFDRKLRQMIEILYGNKYSEYYLRVIMSQRVSMAFTI
                     FVISFAVYGLTLELPILFIFIAFSGLAYYYFGDRVRKKYKKREQEIMADYPQVLSELA
                     LLTNAGMIVRDAWQKVAASKEGALFDEMKTVAVDLNNGVSEPDAFFSFGMRCGVPEIK
                     KFTSTLIQGLTKGNRELGFFLKQQSKEVWEARKFNVKILAEKASSKLLIPITLMFLGI
                     LIMIIVPIFSNLGV"
     gene            5208..5435
                     /locus_tag="PLA01_00795"
     CDS             5208..5435
                     /locus_tag="PLA01_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp1 family type IVb pilin"
                     /protein_id="HPC99869.1"
                     /translation="MGNLLYCAGFCLKQKLGKLFKDERGEVNIVAIVVLIGIALVLAL
                     IFKDFILDLLEDLFETISGNATDAVKKPEPK"
     gene            5481..6155
                     /locus_tag="PLA01_00800"
     CDS             5481..6155
                     /locus_tag="PLA01_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99870.1"
                     /translation="MRRMWKRFKNEKAAVSIVEGAIVFPVVLFTVVFLLFLGNAMHEK
                     SKIDSIVVIAAVKGAQTITDPFNTRLVNDGVNSESGVIPPDSGYFKDSKPYRYFFGIN
                     SVESEIRTFVEDKIASDNTRLFEAQRDNAPIVKAKYNNNILAATFSVEVDFSYELPFK
                     WAFMELPPVIEITSKAEVPVNDSPEFIRNTDMVIDYAEQTGAAQKIRDIMKKVGDYFN
                     IFGRKS"
     gene            6179..6961
                     /locus_tag="PLA01_00805"
     CDS             6179..6961
                     /locus_tag="PLA01_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99871.1"
                     /translation="MKQESQRGSLTVEATVSLTVFIFFIITILSLINICRAQAKIAVA
                     VDSVAKEISQYSYFYGISGLHKKHEELAGMAGDTSQSIDNIIGNTNELYDSIQSIAET
                     GMSTDIGDVGSVLESWENISQKAAEGEATLKELEQQVDELCSDPKKLILGIGAIFANE
                     AINDVVSRVIAPPIAKALSKKHFKDSFHTDADSYLESLGVVDGLDGIDFSNSRLFPDG
                     QNKIEIVAVYKVRIIRLFPIESEFVFYQTASTKGWLLGDKKE"
     gene            6997..7665
                     /locus_tag="PLA01_00810"
     CDS             6997..7665
                     /locus_tag="PLA01_00810"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HPC99872.1"
                     /translation="MWTVIAIPLALLWVILSSGYLQKEVIAKKETLKDKALAFFPRKP
                     LYLVALTVVLIMGIMSFGFQYYFFSDYIKAAKLMTLVMMLAPIAYIDFNSMRIPNELL
                     LGGLLTRVLFYVIEILANNKTLFDIFLSDMAGVLLGGGAFLLGALIAKNGIGMGDAKM
                     FAVIGIYCGYTITMTVMVFSLFLCFVSALVLLILRKKGRKDTIPFAPFVFIGTFLTVI
                     LGSY"
     gene            7694..8986
                     /locus_tag="PLA01_00815"
     CDS             7694..8986
                     /locus_tag="PLA01_00815"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HPC99873.1"
                     /translation="MGNKTLSLIFVIFLFAAAWYTVVQKSVQLPLKYNTNIEQARELA
                     RKEIYQKALDHYKAALQLKDDDIGIHIEIAKIYLAINDEWSFEYYCKKMMKEFPKNPE
                     PYEILANYHKEASDYSECFKIINSAKKKHITSEQLETMESEIQYEYVTRYKTYHDVKM
                     FNEALCPVKIKDRWGYAVSGGSLWISTNYEEVGGFVNGIAPVKKDGKVFYIDMSGEKE
                     LVPDENFDYLGNFNEAAPALQDGKYFYVNKEFEKVFGDYDYAGNFNDGYAAVKKSGKW
                     ALINSEGEQITEYVYEDIKLNEMDNCFINGVAFAKENGKYYMLNGSGEKVSQTGYDDV
                     FPFAGSEPTAVKAGGLWGFVDINGEMIIEPRYQAARPFSNGLAAVKIDDKWGYITIDS
                     QVVIPAIYDDAREFTGNGGAAVKTGDNWVMINLCKYMS"
     gene            9002..10489
                     /locus_tag="PLA01_00820"
     CDS             9002..10489
                     /locus_tag="PLA01_00820"
                     /inference="COORDINATES: protein motif:HMM:NF039422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6382 domain-containing protein"
                     /protein_id="HPC99874.1"
                     /translation="MSNKLNFTYENIGAKNFLVYNLDISDEVDTVTSGMLLNNKIEGL
                     IPYTFSQLDTSRSFKYNITSKVTLNNYLTGNVTRKRLVGVLQSITNAILEAEEYLIDI
                     NSFIFDPEYIYVDVSSYSGYLVCLPLMNMSTEKVSFDEFIKEIMYSVQFDQSENCDYV
                     AKIISFLNSRRPLSIPDFKRLLGGLMVVGSGSKPSEQKQNQATAFPPNAMPNVASNMT
                     LNEGSNMAPNIGPNATPNIVPGKPVIEPRKQNMDKPVRTETNKQQSGFNMGFDMPELP
                     GKKTAGSPNQDDKKESKKGSFLNSIFGSKKKPKQERPVQVPENNKNVTGQVVMPPINK
                     NTAGPEIPQRPITNDVPRREFPAENKEIPQRENKDMLFDNDDMSPLERTIVLNPDDLR
                     QNREPVFPYLVRKRTNEKININKEIFKIGTEGRYVDYVCINNHAISRSHADIITKNNE
                     YFIRDNNSTNGTFVNGERVLGNQEVKLTHEDKIKLADELFEFRIY"
     gene            10506..11273
                     /locus_tag="PLA01_00825"
     CDS             10506..11273
                     /locus_tag="PLA01_00825"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HPC99875.1"
                     /translation="MDYLKVLLTDVGISRKVNEDSLYVDVIVSENKKALFSIICDGMG
                     GLSEGEVASSSAVRAFYYWSKSNLPEMLKNQIIFEELKCQWDEIIFEQNQILSEYGRR
                     KGIRLGTTLTALLIVDYKYYAVHVGDSRIYEISDGVYQLTSDHSVVARDVRMGNITLE
                     QAKNDPRRNILLQCIGASETVEPEYLTGEAVKDTVFMLCTDGFWHNVTEDEIYSNLNS
                     SELLNEDIMLDRARYMIEINKSRLETDNISIILVRTF"
BASE COUNT         3739 a         1644 c         2624 g         3345 t
ORIGIN      
        1 taagtttttt aaaattgatg tcaaggtaaa taagaagctg gatgatataa ataatgtgga
       61 catcaacctt aagtatgtaa agggatatta tgcacaaagc tatgcaggaa caggagaaca
      121 cacagatagt gacggagaca ttcccgacgg cgaagtaagc aaagctgtca tatccgatcc
      181 taccggtatt gcagtatcgg gagacagaat ttatgtggcc gacagaaggc tgagggtgat
      241 tgaaaacggc gaaataaaca caatgcccat ggatatggat gtgggaactg ttagaaccga
      301 tttgaacggt aatgtatatt tttcccttct gccttggctt gatgaggatg aacaaatgct
      361 tacgggaatt ttcaagctgg ataatgaagg ggcaaacagt gtatatgtta tagacgagcc
      421 gttctccaac ataggagatt ttgcctttga cagccagggt aacatgtacg tcctgataca
      481 ccaagagata ttgaatgata tgtctgagca gaccaagatt gtatatgtgg acatccagac
      541 tcaaaatgct gttgtgatgc gggaaattgg aggaataatc gacagcataa cagtggacgg
      601 cgatgacaat gtttatatat catcttctgt aaacggtgct atttacaaat gggaaaaggg
      661 agaagaagag tttaaatatt ttgccggcaa ggaaaatgac aggcatttta ttgacggaat
      721 aaacaacagg ttctttgccc ccgtaaaaat tgctgccaga gaagactttt tatatgtcat
      781 tgaccaaaat gtaataagaa gaatagcttt gaaggatggc aagctggttg atgttgagac
      841 aatagcagga caggcagggg acggagagaa aaatttggac aaacaggaag gtgtggatgc
      901 ctggtttgac agaccggtgg atataacttt cgatgatcag ggcaatattt atgtgtccga
      961 taaggatgct tatataattt ggaaaatact taatgaagaa taaaggagaa aatattgcaa
     1021 gttttggcta tttgctatat aatattacaa tataacaacg caaaaaaaat gtctaacatt
     1081 gatataatgc gtagaataac cattaagggt gtatggaatg attagaataa aacttgttat
     1141 acttgagcgg gatgaaaatt acataacaag aataaccaat gcaattaatg caaactatag
     1201 tgacaaactg gagttgtttg cttttacaaa gcaggatact tttattgact ttattaagaa
     1261 caataaagtc gatataatac tggcttcgga tacctttgat gttaatttga aggaaatccc
     1321 tgagagaatt cagtttgctt attttgtgga ttcccccagt ttggaatcgt taaacaacaa
     1381 aaaggctatc tgcaagtttt ccaagatcag cttgatttat aaagagattc ttaaccttta
     1441 ttctgaaaac ccggtgaatg tttttaattt aaaaaaggat ggatcggggg aaaccggaat
     1501 tttgacgttt ttaccatgta acggcggaga cggagcgagt gttatggcgg ctgcctgctg
     1561 tcttaatttt gccgatagag ggagaagggt tttatatctt aaccttgaaa gctttggaac
     1621 accggacata ttttttgatg ccgaaggaac ttttaattta agcgatttga tttactccat
     1681 caaaagcaga aaatcgaaca ttgccttgaa aatagaaagc atggtaaaga aggataagag
     1741 cggagttttc ttttttgacg agtgcaaagt gcctcttgat ttaatggaaa tgaccggtga
     1801 ggatattcaa atactgttcg acgttatcaa tgaaagcaat ttatttgatt atgttgtagt
     1861 agatacggaa atggctttaa atgatgttat attgcaaata atgcaaaatt ccaacaggtt
     1921 gatttatgtt accgatggtt ctcaaacttc gaacttgaag tttaaacgta tttattcgac
     1981 ccttgaaatt gttgagggac agcgaaatct tagcttattg ccgaaaatat cgatagcata
     2041 taataagacc aactgtatat cgaataaata catagaagaa attcagaata taaatgtttt
     2101 aggttgggca ggagaatgca tcaaggcttc tagtgcccaa gcagcacatc aaatatcgaa
     2161 aatgtcgatg tttgatactt tactgatata ggtgggagta tgtatggatg aaaataagct
     2221 gcacgaaata gttatttcga tcagagacca tatttatgca aatttgtctt tgagtgagct
     2281 tggggatgaa gaactccggc aggaaattga gaatatcgtt ttacagagga cttcggatac
     2341 atatttgtct attgaggata aaatcagtat tatcgaacag gttttcagtg ccattcgcgg
     2401 gctgggggtg ctggactcta ttattgatga tgagtctatt actgaggtca tgataaacgg
     2461 cccggataat atctttattg aaagaaaagg aaaggtagca aagcttgatc agaagtttga
     2521 aagcggaagg aagcttgaag atgttatcca aaagattgta ggttcggtgg gaagagaggt
     2581 caatcaagcc aatcccattg tggatgcaag gcttaaagac ggctcaaggg ttaatgtggt
     2641 tttgccgcct atttctctca aaggacctgt agttactatc aggcggtttt caaaaacccc
     2701 gatgactatt gaaaagctga tagaatttgg ctcgataacg gaagagattg ccgagaagtt
     2761 ggagctgttg gtcagagcaa aatataatat ttttataagc ggaggtaccg gttccggtaa
     2821 aactacgttt ttgaatgccc tttccaactt tataccgaag gatgagagaa taataaccat
     2881 tgaggactca gcggaactgc agattaaagg ggttgataat ctggtgtccc ttgagactag
     2941 aaatgcaaat gttaccggaa gcggtcgaat aagcataagg gatttgataa aagcatcttt
     3001 gagaatgcgt cctgacagga ttgttgtagg agaggtaaga ggggaagagg cattggatat
     3061 gcttcaggca atgaataccg gtcatgacgg ttcactgtcc actggtcatg cgaactccac
     3121 aagggatatg ctgtctcgtt tagaaacaat ggttttaagc ggttcatccg ggttgccttt
     3181 ggatgctgtc cgccagcaaa ttgcttctgc gattgacgta atgattcact tggcaaggct
     3241 gagggatagg tcgagaaaaa caatggagat tactgaaatt gtgggctatg aaaacggaaa
     3301 gataatttta aatcctcttt atgtttttga agaggatgaa aataccacac tagagaaggt
     3361 ttcggggaaa ctcaaaagga cgaataaccc gatggtaaat gttgataaat taaggctttc
     3421 cggaataaat acagtgatat aggaggcaaa gcatggaata tacggattac tctgtttata
     3481 aaatgaaagc ttctgagaaa atcatgtatt ttcttttagc ttttgcggtg ggggcgtttg
     3541 tcggatatgt attctatggt ggtataggaa aggatgctga cggaaatccc actacactaa
     3601 cccatatcct gaacattatt atatccggaa ctaccggtat catagcaggc agatttttca
     3661 taccgataat gactaagcaa attattaaaa agaggaagaa aagtttgcat ttgcagttta
     3721 gggatatgct tgaatcaata acagcaagct tgaacgcagg aaggaatgta ccggattctt
     3781 ttctcagtgc ctatgacgat ttgaaggtgc aatatccgga aggtgcattc atcttaaaag
     3841 aactggaaat aattctttcc ggcctagaga ataatgttaa tattgaaaag ctgttgcttg
     3901 accttggaaa acgaagcggg aacaatgata tcatgagctt tgcaagcgta tttgaaattt
     3961 gctaccgtaa gggcggaaat attaaggatg taattaaaga cacccatcag ataataagcg
     4021 ataaaattga aatagagatg gatatagaga caatggttac ttcgaacaaa atagagcaat
     4081 atatcatgac gattatgccc atagcattag tcggagttgt taagataatg agtccggatt
     4141 ttggtgataa gtttgtttca cctatgggca ttatatcaac gaccattgcg gttggcatat
     4201 ttgtagtagc atactttatt ggaaaagaaa tattgtcaat aaaaggggtg tgaggttaca
     4261 cattatgttt atttcactcg aagttgttgt attgcttggc ataggaacat ttttttcgtt
     4321 gatttgggca gtattgctgt taacttcagg aaaatataac gagctagttg ctcctttgga
     4381 taaaaaagag tttcctctaa aggagctgta tcctttaggg tataaaattt tagatataat
     4441 aaaatacggt tacaacacaa aattcgacag aaaattaaga caaatgatcg agatattata
     4501 tggaaataaa tactctgagt actacttgcg agttattatg tctcagaggg tgtccatggc
     4561 atttacgata tttgttatat cttttgcagt ttacgggtta acacttgaat tgcctatatt
     4621 gtttatattt attgcgtttt ccggtttggc ttactattat tttggagata gggtcagaaa
     4681 aaagtataaa aaaagagaac aggaaataat ggcggattat ccgcaggttt tatcggaatt
     4741 ggcactactc acaaacgcag gaatgattgt tagggacgca tggcaaaagg ttgctgcgtc
     4801 aaaggaagga gcattgtttg atgagatgaa aactgtagct gtagatttga ataacggcgt
     4861 ttcggaaccc gatgcttttt tctcctttgg tatgcggtgt ggtgtgccgg aaattaagaa
     4921 atttacatca acattgatac agggattgac aaagggtaat agggagttgg gcttttttct
     4981 caaacagcaa agcaaagagg tttgggaagc aagaaaattt aatgtaaaaa ttttggctga
     5041 aaaggcatca agtaagctat tgatacctat aacattgatg tttctcggta ttttgattat
     5101 gataattgtg ccgatattct ccaatttggg agtgtgacat aggcggtttg gttctaactt
     5161 aattagaact taattatatt aaaaaaatcg gtaaaggggt gtttattatg ggaaatcttt
     5221 tgtactgtgc aggtttttgt cttaaacaaa agctgggcaa gctattcaaa gatgaaagag
     5281 gagaagtaaa tattgtggca atagttgtgc ttattggaat tgcacttgtt ttggcactga
     5341 tttttaaaga ttttattttg gacttgcttg aggatttgtt tgagacaatt tcaggcaatg
     5401 ctacggatgc agttaaaaag cctgaaccaa aatgattatg ggattaaaca atcgaaagtc
     5461 acgaggataa ttgaggcgat atgagaagaa tgtggaagcg ttttaagaat gaaaaagctg
     5521 cagtttcgat tgttgagggt gcaattgtat tcccggtggt tctgtttaca gttgtattcc
     5581 ttctattttt aggaaatgca atgcatgaga aatcaaaaat tgactcaatt gtagtgattg
     5641 cagctgtaaa aggagcacaa actattactg acccgtttaa tactagactt gttaatgacg
     5701 gggtaaattc tgaatctggg gtaattcccc cagattcggg atattttaag gattcaaaac
     5761 cctacaggta tttttttgga ataaacagtg ttgaaagtga aattcgaacg tttgttgagg
     5821 acaaaatagc cagtgacaac accaggcttt ttgaggcaca aagggacaat gcaccaatag
     5881 taaaagcaaa atataataat aatattttag cggctacatt ttcggttgaa gtggatttta
     5941 gttatgaatt gccttttaaa tgggcattca tggaactacc gcctgttata gagattacat
     6001 ctaaggccga ggtgcccgtt aatgattcgc cggagtttat tcgtaacacc gatatggtaa
     6061 ttgattatgc tgaacagaca ggggcggcac aaaaaattag agatattatg aaaaaagtcg
     6121 gtgactattt taatatattc gggcgaaaat cataatacca aggaaacggt gaggtcaaat
     6181 gaaacaagaa agccagcggg gatctttgac agtcgaagcg acagtgtctt tgacagtgtt
     6241 tatttttttt ataataacaa ttctgtcgct aatcaatatc tgccgtgccc aagcaaagat
     6301 tgcggttgct gttgacagcg tagcgaagga gatatctcaa tacagctatt tttatggaat
     6361 ttcaggcttg cataaaaagc atgaagaatt ggcaggaatg gccggagata cttcgcaaag
     6421 tattgataat attataggca ataccaacga gctgtatgat tcaattcaga gtattgcaga
     6481 aaccggtatg agtacggata taggtgacgt aggcagcgtt ttggaaagtt gggaaaatat
     6541 atcgcaaaaa gctgcagaag gtgaagctac tttgaaagaa cttgagcagc aggtagacga
     6601 attatgcagc gatcctaaaa aactgattct gggaattggt gcaatttttg ctaatgaagc
     6661 aataaatgat gttgtttcaa gagttattgc gccacctata gcaaaggcac tgagtaaaaa
     6721 acattttaaa gactcgtttc atactgatgc tgatagttac ctggagtcac tgggtgtggt
     6781 tgacgggctg gacggaattg atttttcgaa ttcaaggttg tttcccgatg gtcaaaacaa
     6841 aattgaaatt gtagctgtat ataaagtgag aattattcgg ttgtttccta ttgaaagtga
     6901 gtttgtattt taccagactg catcaacaaa gggttggcta ttgggagata agaaagagta
     6961 aagttttaat tacatagatt ttaaggagaa tatttaatgt ggacagtgat tgcaattccc
     7021 ttggcattat tatgggtgat attgtcaagc ggatacctgc aaaaagaggt aatagcaaaa
     7081 aaggaaacac ttaaggacaa ggctttggcg tttttcccac gaaaaccgtt atatcttgta
     7141 gctttgacgg ttgtacttat tatgggaatt atgtcctttg ggtttcagta ttattttttt
     7201 tcagactata taaaggcggc caagcttatg acgttggtaa tgatgcttgc ccctattgca
     7261 tacatagatt ttaatagcat gaggattccc aatgaactat tgctgggcgg gttgttgacc
     7321 cgtgtactgt tttacgttat tgaaatactt gcaaacaata agacattatt tgatatcttt
     7381 ttatccgata tggctggtgt tttattggga ggaggagctt tccttttagg agctctcatt
     7441 gcaaaaaacg gaattggcat gggagatgcc aaaatgtttg ccgtaatagg tatctattgt
     7501 ggttatacga tcacaatgac agtgatggta tttagtttgt ttttgtgttt tgtttcggca
     7561 ttggtgttgt tgattttaag aaaaaagggc agaaaagata cgatcccctt tgcaccattt
     7621 gtttttatcg gtactttttt aactgttatt ttaggaagtt attaacactt tgcattttga
     7681 tgaggtggca ttgatgggca ataaaacatt aagtttgatt tttgttatat ttttatttgc
     7741 tgcagcatgg tacactgtgg tacaaaaaag tgttcagctg ccgttgaagt ataataccaa
     7801 tattgagcag gccagagaac tagcgagaaa ggaaatctat caaaaagcac tggatcacta
     7861 taaagcagcg ttacaattaa aggacgatga tataggtatt catatagaga ttgcaaagat
     7921 ttatctcgct ataaatgatg aatggtcttt tgaatattac tgtaaaaaaa tgatgaagga
     7981 gtttccgaaa aatccggaac cttatgaaat tttagctaat taccataagg aagcttccga
     8041 ttattccgaa tgctttaaaa ttataaatag tgctaagaaa aaacatatta catctgagca
     8101 gttagaaaca atggaaagtg aaattcaata tgaatatgta actcgctata agacttatca
     8161 tgatgttaag atgttcaatg aggcactgtg tccagtcaaa ataaaagacc gttggggtta
     8221 tgccgtttcg ggtggaagct tgtggataag tactaactat gaagaagtgg gaggatttgt
     8281 gaacggtatt gctcctgtca agaaggatgg aaaagtgttt tatattgata tgagtggtga
     8341 aaaagaattg gttccggatg aaaatttcga ctatctcggc aattttaatg aggcagcacc
     8401 ggctttacag gacggaaaat acttttatgt caataaagaa tttgaaaaag tatttgggga
     8461 ctacgattat gccgggaatt tcaacgacgg atatgcagcc gtaaagaaat caggaaaatg
     8521 ggcacttata aacagtgaag gcgaacaaat aaccgagtat gtttatgagg atataaagct
     8581 aaatgaaatg gataattgtt ttattaacgg agttgctttt gcaaaagaaa acggaaaata
     8641 ttatatgctg aacggctctg gggaaaaggt ttcacaaacg ggttatgacg atgtttttcc
     8701 ctttgccggc agtgaaccta ctgctgtgaa ggcaggtgga ttgtggggat ttgtagacat
     8761 taacggagaa atgattatag agccccggta tcaggcggct agacccttca gtaatgggct
     8821 tgctgcagta aaaattgatg ataagtgggg ttatataacg atagacagcc aggtcgtgat
     8881 tccggctata tatgatgatg ccagagagtt tacaggaaat ggaggtgcag ctgtaaaaac
     8941 tggcgataac tgggtaatga ttaatctatg taagtacatg agctagtttg ggggcgtgtt
     9001 tatgagtaat aagttgaatt ttacctatga aaatatagga gcaaaaaatt tccttgttta
     9061 taatttagat atttcagatg aggtggatac tgtaacttcc gggatgcttc tgaataataa
     9121 aattgaaggg ttaataccct atactttttc gcagttggat accagcagga gcttcaaata
     9181 caacattacc tcaaaggtta cgctgaataa ttacttaact ggaaatgtta cgagaaaaag
     9241 gcttgtggga gtacttcaga gtattaccaa tgcaattttg gaagctgaag aatatctgat
     9301 agatataaat agctttattt tcgatccgga atatatatat gtcgatgtat ctagctattc
     9361 cggttatctg gtatgtttgc ctttaatgaa catgagcacg gagaaggttt cctttgatga
     9421 gtttattaaa gaaatcatgt acagtgttca gtttgatcaa tccgaaaatt gtgactatgt
     9481 ggcgaaaata atcagctttt taaacagtag acgtcctctc tcgattccgg atttcaagag
     9541 gcttttgggt ggacttatgg tggtaggctc cggtagcaaa cccagtgaac agaagcaaaa
     9601 tcaagctaca gcttttccgc ctaatgcaat gccaaatgta gcttcaaata tgacgctcaa
     9661 tgaaggatct aatatggcac ctaatatagg acctaatgca acacctaata tagtgccagg
     9721 aaaaccggtt attgagcctc gcaaacagaa tatggataaa ccagtcagga ccgaaaccaa
     9781 caaacagcaa tccggcttta acatgggctt tgatatgccg gagttgcctg gcaagaaaac
     9841 cgccggttca ccgaaccagg atgataaaaa ggaatcaaaa aaaggcagct ttttgaattc
     9901 cattttcggt tcaaagaaaa agcctaaaca agaaagaccg gtgcaagtac ctgagaataa
     9961 caaaaatgta acaggccaag ttgttatgcc tccaattaat aaaaacaccg ccggtccgga
    10021 aattcctcaa agaccgatta caaatgatgt gcctcgaaga gagttccccg cagagaataa
    10081 agagattcct caaagggaga ataaagatat gttgtttgat aatgatgata tgtccccatt
    10141 ggagaggact atcgtcctta accctgacga tttacgtcaa aatagggaac ctgtttttcc
    10201 ttatcttgtt aggaaaagaa ccaatgaaaa aattaatata aataaggaaa ttttcaaaat
    10261 cgggacagaa ggaagatatg tggattatgt ttgcatcaat aatcacgcca ttagccgaag
    10321 tcatgcggat ataataacta aaaacaacga gtattttata agagacaaca actctaccaa
    10381 cggaacattt gtcaatggag aaagggtatt gggaaatcag gaagtaaaat taacccatga
    10441 agacaaaata aagcttgcag atgaattgtt tgagtttaga atatactaaa ttggcaggta
    10501 gatatatgga ttacttaaag gttttgttga cggacgtagg tatatcaagg aaagtcaatg
    10561 aggattcatt atacgtggat gttattgttt cagaaaataa aaaagcattg ttttcaatta
    10621 tatgtgacgg tatgggagga ctttccgaag gagaagttgc aagttcatcg gcggttaggg
    10681 cattttatta ttggagtaag agcaatctgc ctgaaatgct gaaaaaccaa atcatttttg
    10741 aagaattgaa gtgccagtgg gatgaaatca tattcgagca gaatcaaatt ctttccgagt
    10801 atggaagacg caaaggtatc agacttggca ctacgctgac ggctttactc atagttgatt
    10861 ataaatatta tgctgtacat gttggtgaca gcagaattta tgaaatatcc gacggagttt
    10921 atcagctcac atcggatcac tcagttgttg cccgggatgt aaggatgggg aatattactt
    10981 tggaacaggc aaaaaacgac cctagaagga atattttatt gcagtgtata ggggcgtcgg
    11041 aaacagtaga accggaatat ttgactggtg aagctgtgaa agataccgtt ttcatgcttt
    11101 gtaccgacgg attttggcat aacgttacgg aggatgagat ttattcgaat ctaaatagtt
    11161 ctgaactctt aaatgaagat attatgctcg atagggcacg atatatgatt gagattaata
    11221 aatcgcgatt ggaaacggat aatatttcga ttatattggt aagaacgttc tagatttttg
    11281 cggaaattac ataaatacgt aagataatat tgcaaaataa tacttgttgt tgtataatgc
    11341 aatatggcat tt
//
LOCUS       DAOOJC010000015        11285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_5245_length_11285_cov_4.191184, whole genome shotgun sequence.
ACCESSION   DAOOJC010000015 DAOOJC010000000
VERSION     DAOOJC010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 11285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11285
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5245_length_11285_cov_4.191184"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3999)
                     /locus_tag="PLA01_00830"
     CDS             complement(<1..3999)
                     /locus_tag="PLA01_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HPC99876.1"
                     /translation="MNKSRRFLSVIIAIELLLIPLLQVDVFAAALPINISVVNYTQTG
                     GLTISWQNPAGAQSCQITYHKPDGSEATEMLAAVSSYTLLELQNDYIYDICLEVFSGA
                     GGTGEVIGKGFLFFLPRISFYVSRVSQRKIELPGGGYEIGSEPQLNLKWVMPKIWNGV
                     DAVIDADDPQALSLINNSLNAVYDGGLDIESLDFKINISTDYSLLNSGSTNAAVLVES
                     NAGSYEAHVSGNSAITASVSDSGGFLSFDLLGRKDEFASFPVLTGDESDPFPANVLPD
                     GDIFPGTVYYMNIKLDFKNDEGETKFAVYTGKPVDYNGSLIIGDYPYTYTPIRFQLSR
                     DAANNVYVRVYRVNQGSLDLPRMFYEVQAIGDSSFPGDWPKKKTMDDSFFPPGTDSAI
                     TLISGVSPNNIIYYKIVVKTESTLDRIESLPMDYILAEDTSKPPVPEDITIIRRKPVE
                     GNVDINGDGIVDDGTLRKSTDVTISWKKPANWDEIKANTDPDKDIVFHVLLSTSQAEI
                     DVQPYPELKAEGESYGFYPLKYRRVLYFSSKSVIENGNRLEYTIKGLELFKGNYFDGM
                     DGTEPVILPENIANSEEYPSFLLPNTVYYIQMYTTASSERNSTDVEDMSDKSVIVSFT
                     TLSIKNVEVPLPKNLRITKNNADVTIGETIDVSNYVEIQFGKVNIDWNDYTDKTTVQK
                     AVYYDIYMSTNPDINSFRLIGTTEQTDGDLIFTGVDDKKSNTVRAEIRNFSPGTPAYT
                     AFGDKLRPNTTYYFYVKTRLAIGEENRESVPSAAIAVTTVKGISGEPDDSSKKPLAPT
                     DFAIAEDENGNAMVSGSKVVLTWKKQENDVVYVIVCSSRRIEPDEIFNMSSDPVSESF
                     NEEFGDIILDPLAPELSQGFEYNPSTMECRFTVDRWLFPNRLYYFSIRAVNREDGTIY
                     SQWVSIPVTTNPTEQPEYLNVVSDVQLGFFFSDADITARAEDYKIYLRSKSSMDFTYL
                     PRNKYTIFRQGSVCFVRLINLKPDTYYDVSVYKEGSQTPVFIEKDMKTRNDRHEVEVV
                     WRGVYGYGYEIAVKTPFDDEYTVLSHENLEEYRVSDGRILPYYTEEDARTNGTNFEYC
                     YARIKSIPVETGRGSVENIPLSSNMKYYVKVRAVRTDPVNTSVVSYSKYVGPVAARTE
                     FDQDEYDNEDMDNRKEVSLLDKISEFEELLYWRIDIGNGEYNKLLIKGERMVNVIENN
                     GPYPFVLDISDISQGIDTDIIYIPDNVIKALDKENKSLVIKTTGAEFTIRPQTINTQN
                     TGVVNLENRSGVNGIYYKLIISQSIKTARVVPDDAVPASLINSVTLDAVGIGITWSRL
                     EDEIKNKIYNDSS"
     gene            complement(4173..5327)
                     /locus_tag="PLA01_00835"
     CDS             complement(4173..5327)
                     /locus_tag="PLA01_00835"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPC99877.1"
                     /translation="MFYEYIISFVLALIVAFSFTPVARKISFSVGAVDVPKDSRRMHN
                     RPIARLGGLAIFAGFIVSLLFGIICTYLNISGIVPGRELLGLLAGAVIIVAVGIVDDI
                     KQLGPKIKLLVQIIAALTAVLISGTRIVNITNPFVEGGTTQFSDFVSYPLTIIWIVGV
                     TNAVNLIDGLDGLAAGVSSISYLSLFFVSLITGDADSALITVILAGSTLGFLPYNFNP
                     AKIFMGDTGATFLGFTLSIISIQGMLKSYAALSIAVPLLVLGLPLFDTLSTIFRRVLN
                     RKPIMQADRGHLHHRLIDMGYSHKQTVLVMYTLSATLGLSAIVLADKGFISAIILVIT
                     VAAFVIGGAKYMLEIDDIKITGDEKQKEEVVNPIINNQTHGELKVNDKRN"
     gene            complement(5489..5944)
                     /locus_tag="PLA01_00840"
     CDS             complement(5489..5944)
                     /locus_tag="PLA01_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HPC99878.1"
                     /translation="MRPTWDEYFMQIVELIKTRSTCLRRQVGALIVKDKRILSTGYNG
                     APMGCKHCSEIGCLREQLKVPSGQRHELCRAIHAEQNAIVQAAYSGTSVKGGTLYVTN
                     QPCVLCSKMIINAGIKKIVFNGDYPDELAMEMLKEAGIRVIKFEPGDIF"
     gene            complement(5977..6606)
                     /gene="upp"
                     /locus_tag="PLA01_00845"
     CDS             complement(5977..6606)
                     /gene="upp"
                     /locus_tag="PLA01_00845"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HPC99879.1"
                     /translation="MDNVFVLDHPLIQHKVSLLRDKNTQTKEFRELVSEISMLMGYEV
                     TRNMPLKEVEIETPVGVARTKVLSGKKMGIVPILRAGLGMVDGMLRLLPMARVGHIGL
                     YRNPDTLEPVKYYGKLPFDVAEREIVVLDPMLATGGSASAAIDYLKEKGVANIKLMCL
                     IASKDGIKKVNKDHPDVSIFCAAVDEKLDSHGYILPGLGDAGDRLFGTK"
     gene            complement(6621..7070)
                     /gene="rpiB"
                     /locus_tag="PLA01_00850"
     CDS             complement(6621..7070)
                     /gene="rpiB"
                     /locus_tag="PLA01_00850"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPC99880.1"
                     /translation="MKIGIGSDHGGYNLKAKLISYLKENNYDVLDFGTFNTESVDYPD
                     YGLKVAEAVKSGECDRGIVICGTGLGISISANKVPGIRAAVCTNSYMARMSREHNDAN
                     ILALGERVVGLDLAIDIVEAWLKAEFQGGRHEARVGKISEIEKKYSK"
     gene            complement(7162..7665)
                     /locus_tag="PLA01_00855"
     CDS             complement(7162..7665)
                     /locus_tag="PLA01_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HPC99881.1"
                     /translation="MKKILFVCTGNTCRSSMAEGLFNHIVKNEVDGLEDYSAVSAGIF
                     AYEGDGANPKSIKVLKEGYGIDITSHRARRITNSDMEDAHLILTMTKDHRDTLVELYP
                     GARDKIWTLKEFVSKDESDRNSGNPFEWDIPDPYGMPEEIYERCARTLKEAIDKLILR
                     LKEEKGL"
     gene            complement(7690..8745)
                     /locus_tag="PLA01_00860"
     CDS             complement(7690..8745)
                     /locus_tag="PLA01_00860"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPC99882.1"
                     /translation="MKTEILKIELNGIDREKLRYAAEVLRGGGLVAFPTETVYGLGAD
                     ALNENAVKKIFEAKGRPSDNPLIVHITGKERAGSLTASVPPAAEILMDRFWPGPLTLV
                     LEKSDKIPGVITAGLSTVGLRAPAHPVALALIREAGIPVAAPSANISGRPSPTTPEHV
                     IDDLYGRVDVIIDGGCSKVGVESTVLDVTVTPPMILRPGGVSCEQLKGVLGDVTIDAS
                     LLRKSSENYVPRAPGMKYKHYSPKADVIVVEGEPERVVQKINNLIGEYKQSNTAVGVL
                     STEQTKMFYKDVPVISLGDRQKPETIAANLFKAFRDFDDIGIEVILAEAINNQGIGLA
                     VMNRMKKAAGYNIIKVK"
     gene            complement(8776..9516)
                     /locus_tag="PLA01_00865"
     CDS             complement(8776..9516)
                     /locus_tag="PLA01_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPC99883.1"
                     /translation="MDNLLKVTLIGLISGITGTSIGGLTAFFVRGVNKRFLSFILEFA
                     AGLMTSVVCFELIPEAFSYGGTVLTFAGVFAGVLTIMTVEEFLRRFGAIKQQNEKTGL
                     LKAGILMAIGISLHNFPEGFAVGSSFGASLSLGIMMTTVIIIHDIPEGIAMAVPMRAG
                     GFSKSKAFFITVLSGLPMGVGALLGAVLGGVSQGFIGACLGFAAGAMLYVVYGELVTE
                     SKRLYLGRLSSVGNVMGIICGIIVTLLN"
     gene            complement(9600..10682)
                     /gene="prfA"
                     /locus_tag="PLA01_00870"
     CDS             complement(9600..10682)
                     /gene="prfA"
                     /locus_tag="PLA01_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPC99884.1"
                     /translation="MFERFQAAENRYDEINHKLSDPAVIANQDEYRKLMKEHAELEEL
                     VTKYGEYKKVSQEISDAKEMLKEKLDREFHEMVETELKEAQEKLEVIKKEMKILLLPK
                     DPNDEKNVIVEIRGGAGGEEAALFAGDLFRMYTRYAERNRWKTEMLDSNPTEIGGFKE
                     VVFSIEGSGAYSRLKFESGVHRVQRIPSTEAGGRIHTSTVTVAVLPEVEEVDVDINPN
                     DLRIDTYRASGAGGQHVNKTDSAIRITHLPTGLVVTCQDQRSQYKNKDKAMAILRSKL
                     YEMAQSEKTNEIAQERKSQVGTGDRSERIRTYNYPQGRVTDHRIGLTLYKIDEILDGD
                     IDEIVDALITTDQAERLGSGGTDYED"
     gene            complement(10944..>11285)
                     /locus_tag="PLA01_00875"
     CDS             complement(10944..>11285)
                     /locus_tag="PLA01_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99885.1"
                     /translation="VSEYNSGIGILSNAVRNMGGEDYAQMYLSYLMPNSGYEDIATKH
                     SISSDNSPFISVTKDMATAIQFSKQTGLFATISTNRKVFFNPFNVFGENEYLVPIVIL
                     FPEIISVSPAY"
BASE COUNT         3027 a         2861 c         1969 g         3428 t
ORIGIN      
        1 cgaactgtca ttgtatattt tgttttttat ttcgtcttcc agtctcgacc aggttatgcc
       61 aatcccgaca gcatccaatg tcacactgtt gatcagcgat gccggcaccg catcatccgg
      121 cacaaccctt gccgtcttta ttgattgact tattatgagc ttatagtata taccgttgac
      181 accggatcta ttctccagat taaccacacc ggtattttgg gtattaatgg tttgaggcct
      241 tattgtaaac tccgcaccgg ttgttttaat gacaagactc ttattctctt tatccaaggc
      301 ctttataaca ttatccggga tatatattat atccgtgtca attccttgtg aaatatcgga
      361 tatatctaaa acaaagggat aaggaccgtt gttttcaata acatttacca ttctttcgcc
      421 cttgattaaa agcttgttgt actcgccgtt tcctatgtca atcctccagt acagaagttc
      481 ttcaaattca cttatcttat caagcagcga aacttccttg cggttatcca tatcctcatt
      541 atcatattca tcctggtcaa attccgttct tgcggcaaca ggccccacat acttggaata
      601 tgatactaca gaagtattta ccggatccgt tctgactgcc cgcaccttaa catagtactt
      661 catgtttgaa ctaaggggaa tgttctcgac acttcccctt ccggtttcca caggaatgga
      721 ctttatccgt gcataacaat attcaaagtt tgttccgttg gttcttgcat cttcctcggt
      781 atagtaggga agtattctgc cgtcactaac tctatactcc tccaggttct catgggaaag
      841 cacggtatat tcatcgtcaa agggggtttt tacagcgatt tcgtaaccgt acccgtaaac
      901 ccctctccaa accacctcga cctcatggcg gtcgtttctc gtcttcatgt ctttttcaat
      961 gaacacaggg gtttggctgc cttctttgta cacgctaaca tcatagtagg tatccggctt
     1021 aaggtttata agtctcacaa agcacactga accctgccgg aaaattgtat atttattcct
     1081 gggtaaatag gtgaagtcca tactgctctt tgatcttaga tatattttat aatcctcggc
     1141 tcttgcagtt atatccgcat cgctaaagaa aaatcccagt tgtacatcgc tcaccacatt
     1201 caaatactcc ggctgttccg tcggattggt tgtaacagga attgaaaccc actgactgta
     1261 tattgtaccg tcctccctgt tcactgcccg tatgctgaaa taatagagcc tgtttggaaa
     1321 gagccagcgg tctacggtaa acctgcattc catggtcgaa gggttatatt caaaaccctg
     1381 tgaaagctcc ggtgccaagg gatcgagtat aatgtctcca aattcttcat taaagctttc
     1441 cgatacagga tctgaagaca tattaaaaat ttcatccggc tcaattcttc tggaactgca
     1501 tacaatgaca taaaccacgt cattctcctg cttcttccat gtcagcacca ccttagagcc
     1561 gctgaccata gcattcccgt tttcatcctc cgctatcgca aagtcggtgg gtgcaagagg
     1621 ttttttcgat gaatcgtcgg gctcgccgga aatccccttt acagttgtaa cggcaatagc
     1681 tgcggaaggg acagattccc tgttttcttc gcctattgca agccttgtct ttacataaaa
     1741 gtaatatgtg gtgttgggtc ttagcttatc gccaaaggct gtataggccg gagtacccgg
     1801 agagaaattc cttatttctg ccctaacggt attgcttttt ttatcgtcaa ccccggtaaa
     1861 tatcagatcc ccatccgtct gctccgtggt tccgataagc cggaaagagt ttatatccgg
     1921 attggtgctc atgtagatgt cgtaataaac cgccttttga accgtggttt tgtcagtata
     1981 atcgttccaa tcaatattca ccttgccaaa ctggatctcc acataattgg atacatcaat
     2041 ggtttcaccg atggtaacgt cggcattgtt ctttgttatc ctcaggtttt tagggagggg
     2101 aacctccaca ttctttatcg aaagagtggt aaaactgact attacggatt tatccgacat
     2161 atcctccaca tctgttgaat ttcgttcact tgaagctgtt gtatacattt gaatgtaata
     2221 taccgtattt ggaagcaaaa atgaaggata ttcttcactg tttgcaatgt tctccggcaa
     2281 aattaccggc tccgtgccgt ccattccatc gaaataattt cccttaaaca gttcaagtcc
     2341 ttttatcgta tactctagac ggttaccgtt ttctattacg ctcttggatg aaaaatacaa
     2401 tacccttctg tatttcaggg gataaaaacc gtaactctcc ccctcggcct taagctccgg
     2461 atacggctgt acatctattt cggcctggct tgtgctcaat agtacatgaa aaactatatc
     2521 cttgtcgggg tcagtgtttg ccttgatttc atcccaatta gcaggctttt tccacgatat
     2581 agtcacgtct gtggatttgc gaagggtacc gtcatccact ataccatcac catttatatc
     2641 cacattgccc tccacaggtt ttcttcgaat tattgtgata tcctccggaa caggcggttt
     2701 actcgtatcc tctgccagaa tataatccat cggcagtgac tcaatcctgt ccaatgtgga
     2761 ttctgttttt acgacaattt tatagtatat tatattgttt ggtgaaaccc cggaaataag
     2821 ggttattgca ctgtccgtac cgggcggaaa gaaactgtcg tccatggttt tctttttcgg
     2881 ccagtcccca gggaaggaag aatctccaat tgcctgaacc tcatagaaca ttctcggcag
     2941 atcaagactt ccctgattaa ccctgtaaac ccttacataa acattatttg cggcgtctct
     3001 cgaaagctga aaccttatag gcgtataggt ataagggtaa tcacctataa taaggcttcc
     3061 gttataatcc acgggtttcc cggtataaac cgcgaactta gtctctcctt cgtcgttttt
     3121 aaaatcaagc ttaatattca tataataaac cgttcccggg aaaatgtccc catcgggaag
     3181 cacattcgcc gggaagggat cagactcatc cccagtgagc accggaaagg aagcaaactc
     3241 atcctttctg cccaatagat caaagctcaa aaatccgccg gaatccgaaa ccgaagcagt
     3301 tattgcggaa ttgccggaaa catgtgcttc ataacttccc gcattacttt ccaccaacac
     3361 tgcggcattg gtggaaccgc tgtttaaaag ggaatagtca gtagaaatat ttattttaaa
     3421 atccaggctt tctatatcca atccaccgtc atacacggca tttaaactgt tgtttataag
     3481 acttaaggcc tgagggtcat cggcatcgat taccgcatcg accccgttcc atattttagg
     3541 cataacccac ttgaggttca gctgaggttc acttccaatc tcatagcctc ctcccggaag
     3601 ctctatcttt ctctggctta ctctgctgac ataaaaagaa attctgggga ggaagaataa
     3661 aaaccctttt ccgatcactt ctcccgtacc gccggctccg ctgaatactt ccaggcaaat
     3721 atcatatata taatcgtttt gcagttcgag caatgtgtaa gaactgacag ctgccaacat
     3781 ctccgtagct tcacttccat cgggcttgtg ataggttatt tggcaggact gtgcccctgc
     3841 cgggttctgc cagctgattg tcagcccacc cgtttgagta taattgacaa cggatatatt
     3901 tatcggaagt gccgcagcaa aaacatcgac ctgcaataaa ggtataagca gcagctcgat
     3961 tgctataata acagaaagga atctcctgga tttattcata ggacaacctc caaaggaaat
     4021 catttatcat attaatttat cggtataata aagtagcaac tttagcaact caatgagaca
     4081 gcacaagggg aagcacaagg ggacggttct tccgtgtcgt acgacacgga agaaccgtcc
     4141 ccttgtgcta cttatataaa aattttacag cattagtttc tcttgtcgtt gaccttcaat
     4201 tccccatggg tctggttatt gataatggga tttacaacct cttccttttg cttctcgtcc
     4261 ccggtaattt ttatatcatc aatctccagc atgtatttgg caccgccgat tacgaaagct
     4321 gcaacagtaa taactaagat aattgcactt ataaagcctt tgtccgccag aacaatggca
     4381 ctgagtccta aagtggcact taatgtgtac atgacaagca ctgtctgctt atggctatac
     4441 cccatgtcaa taagcctgtg atgcaaatgt cccctgtctg cctgcatgat cggctttctg
     4501 tttaaaactc ttctgaatat tgtagacagc gtatcaaaca acggaagtcc cagtacaagc
     4561 aacggaactg ctattgaaag agcagcataa gatttgagca ttccctgaat ggatataatc
     4621 gaaagggtaa atcccaaaaa ggttgctccg gtgtctccca taaatatctt tgcggggttg
     4681 aagttgtacg gcaaaaatcc cagcgtagaa cctgcaagta ttacagtgat cagtgcacta
     4741 tccgcatcac ccgttatcag cgaaacaaaa aacaatgaaa gatatgaaat ggacgatacc
     4801 cctgcagcaa gaccgtcaag accgtcaatt aaattaactg cattggttac accgactatc
     4861 cagattattg tcagcggata ggatacgaaa tcactaaact gtgtggtacc gccttctaca
     4921 aacgggttgg taatgtttac aatccgggta ccggaaataa gtactgccgt caaagctgca
     4981 attatttgaa caagcagttt tatttttggt cctaactgct tgatatcgtc aacaattccc
     5041 acagcaacta ttataaccgc ccctgcaaga agccctaaaa gctccctccc gggcacaatt
     5101 cctgaaatat taagatatgt acatatgatg ccaaacaata aagacacaat aaaccctgca
     5161 aatatagcca gtcctccaag tcttgcaatg ggtctgttat gcatcctcct tgagtccttc
     5221 ggcacatcaa cagctcctac actgaacgaa atcttccttg ccaccggtgt aaatgaaaat
     5281 gctacgataa gtgccagaac aaaggatata atatattcat aaaacacgtt caacaccttc
     5341 tccttttttt atcgcttaat ctttagtaaa ttacacttgg cttttgtgtc ttttttcctt
     5401 aaaagcactt tctcgtgtcc cttttatgtg ttcttcgtct caataagatt atatcatttc
     5461 ataatactaa cctgaactca aactttcatt aaaatatatc gccaggctca aatttaataa
     5521 ctcttatgcc ggcctctttt aacatttcca tggccaattc atcgggataa tcgccgttaa
     5581 atacaatctt ttttataccg gcgtttatga tcattttgga acacaacacg catggctgat
     5641 ttgttacata cagcgttccc cctttcacgc tcgtaccgga gtacgctgcc tgaactattg
     5701 cgttttgctc ggcatgtatt gccctgcata gctcatgtct ctgtccggac ggtactttaa
     5761 gctgttccct caggcaaccg atttcgctgc agtgcttaca acccatcggt gctccgttat
     5821 aacccgttga aagaatcctc ttatccttga caataagtgc ccccacctgc cgtctgagac
     5881 aagtagacct ggttttaatc agttcaacta tctgcataaa atactcatcc caggttggtc
     5941 tcataatctt tcctccctcc aaccatactc aaagtactat tttgttccaa aaagcctgtc
     6001 tcccgcatct cctaaccctg gaagtatata tccgtggctg tccagtttct catccaccgc
     6061 cgcacaaaag atcgacacat ccggatgatc cttgtttact ttctttattc cgtctttcga
     6121 agctataagg cacatcagct tgatattggc cacacctttc tccttaagat agtctatggc
     6181 agcagatgcc gagcctcctg ttgcaagcat gggatccaat acaacaattt ccctttctgc
     6241 aacatcaaag ggaagcttgc cataatattt caccggttca agcgtatcag gattcctgta
     6301 aagacctata tgaccaaccc tcgccatagg cagcaatcta agcatcccgt caaccatgcc
     6361 caagcctgcc ctgagaatag gtacgatccc catctttttc cccgacaaaa cctttgtcct
     6421 ggcaacacca acaggagtct caatttctac ctccttaaga ggcatattcc tggtaacctc
     6481 ataacccatc aacatggata tctcggaaac caattcacgg aactcctttg tctgggtgtt
     6541 cttatccctt aaaagtgata ccttgtgctg aattagagga tggtccaaaa caaaaacatt
     6601 atccatagat tatatctctc ctatttgctg tattttttct caatttcact aatcttacct
     6661 actcttgctt catgccttcc tccctgaaac tcggccttaa gccatgcttc gacaatatct
     6721 atcgcaagat ccaatcctac aaccctctct ccaagagcca atatgtttgc atcgttgtgt
     6781 tccctgctca ttcttgccat atagctgttg gtacacaccg ctgcccttat gcccggaacc
     6841 ttgtttgccg agatcgatat gccaagaccg gtaccgcata ttacaatacc cctgtcgcat
     6901 tctccgcttt taacagcctc agcaacctta agcccgtaat cgggataatc tacagattcc
     6961 gtattaaaag tcccgaaatc caaaacatca taattatttt ctttaaggta gcttattaat
     7021 tttgctttta aattgtaacc tccgtggtca cttccaatgc caattttcat actatcctcc
     7081 aacaatatat tttatagtaa taaaaccttt atgtcaaaaa aagtttaaag caaataaaac
     7141 cctcaggctt cttcaaagca gctataagcc tttttcctct tttaacctca atatcagctt
     7201 gtcaattgcc tcttttaatg tccgtgcaca tctttcatat atctcttccg gcatgccgta
     7261 gggatcagga atatcccatt caaaaggatt tcccgaattt cggtccgatt cgtctttaga
     7321 gacaaactcc ttcaatgtcc agattttatc acgagctccg ggatacaatt cgacaagggt
     7381 gtccctatgg tcttttgtca ttgtgagtat caaatgggca tcttccatat cggaatttgt
     7441 tattcttctg gccctgtggg aagtgatatc gatcccatag ccctctttta agacctttat
     7501 cgacttaggg tttgcaccgt caccttcata agcaaatatt ccggctgaaa cagcactata
     7561 atcctccaac ccgtcaacct catttttcac aatatgatta aacaatcctt cggccatgct
     7621 gcttctacat gtatttccgg tgcacacaaa aaggattttc ttcatgctta cacattcatt
     7681 cctttcaatt cattttacct tgattatatt atagccggcg gcctttttca ttctgttcat
     7741 aactgccagt ccaatgcctt gattattgat ggcttccgca agtattacct caataccaat
     7801 atcgtcaaaa tccctgaatg ccttaaacag atttgccgca atggtctcag gcttttgacg
     7861 gtctcccagt gaaattacag gtacatcctt ataaaacatc ttcgtctgct ctgttgaaag
     7921 cactcccact gcagtattgc tttgcttata ctctccgata aggttgttta ttttttgtac
     7981 aaccctttcg ggctcaccct caacgacaat tacatccgct ttgggggaat agtgtttata
     8041 tttcattccc ggtgccctgg gcacataatt ttccgaactc tttcttagaa gcgaagcatc
     8101 aattgtcaca tctcccagaa cacctttaag ctgctcgcaa gatacccctc cgggtctcaa
     8161 tatcatggga ggagttacag taacatccaa aaccgtggac tccaccccta ccttggaaca
     8221 tcctccgtca attataacat ccaccctgcc atacaggtca tctataacat gctcaggcgt
     8281 agtagggctt ggccttcccg atatattggc actgggagcg gctacgggaa tacccgcctc
     8341 ccttatcagg gcaagagcca caggatgagc cggtgccctc aagccaacgg tggaaagccc
     8401 ggctgtaatc acaccgggta tcttgtccga tttttccagc accaaagtga gaggtccggg
     8461 ccagaacctg tccatcaaaa tctcagccgc cggcggaaca gatgccgtta agctgccggc
     8521 cctttcctta cccgttatat gtactattaa agggttgtcc gacggccttc ccttggcttc
     8581 aaatatcttt ttaacggcat tttcattgag agcatccgca cccagaccat aaaccgtctc
     8641 ggtgggaaaa gccacaagcc ctccgcctct aagaacttct gcagcatacc ttagcttctc
     8701 cctgtcaata ccgtttaatt cgattttcag tatttcggtc ttcatattat tacttcccaa
     8761 catttacttt tgacatcaat ttaataatgt cactattata ccacaaataa tgcccattac
     8821 attccctact gaagacaacc tgcccaaata cagtcttttg gattctgtca ccagttctcc
     8881 gtataccaca taaagcattg cacctgcggc aaatcccagg cacgcaccga taaacccctg
     8941 cgatacgcct cctaagactg ctcccaaaag agcacctaca cccatgggta aaccggacag
     9001 caccgttata aaaaaggcct tcgacttgct gaagcctcct gctctcatag gaactgccat
     9061 ggcaattcct tcgggaatgt catgtattat aataacagta gtcatcataa tacccaggct
     9121 taatgaagcc ccaaaactgg aacctaccgc aaaaccttcc ggaaagttgt gaagcgaaat
     9181 ccctatcgcc atcagtatcc cggcttttaa aaggcctgtt ttttcattct gctgttttat
     9241 agcaccaaac cttcttaaaa actcttcaac cgtcattatc gtgagcacac cggcgaacac
     9301 accggcaaaa gtcaaaaccg ttcccccata gctaaaggcc tcgggaatca gttcaaaaca
     9361 cacaaccgat gtcatcaagc ctgctgcaaa ttcaagtatg aagcttaaaa agcgtttgtt
     9421 cacaccccta acaaaaaaag ccgtcaatcc tccgatactg gttcccgtaa taccggagat
     9481 aaggcctatt aatgtaacct taagtaagtt atccatgttt gcccccggat ataccaatta
     9541 ttaaaactat tacttaattg tattcatatc cggggcacat tattataatg cattattctt
     9601 taatcctcat aatccgtacc gccgcttcca agtctttcgg cctgatcggt agtaatcaat
     9661 gcatccacta tttcgtcaat atccccgtca agtatttcat caattttata aagggtaagc
     9721 ccaatcctgt ggtccgttac cctaccctgg gggtaattgt aggttcttat cctttcactc
     9781 ctgtcacccg taccgacctg gctttttctt tcctgagcta tctcattggt tttctcgctc
     9841 tgtgccatct catagagctt tgacctcaaa attgccattg ccttgtcctt gtttttatac
     9901 tgtgatctct ggtcctggca ggtaaccacc agcccggtgg gcaagtgagt tattcttatc
     9961 gcagaatctg tcttgttgac atgctgtcct cctgccccgg aagccctata agtgtctatt
    10021 ctcaaatcgt taggatttat atccacatcc acttcttcaa cctccggcaa cactgccacc
    10081 gtaacggtag aagtatgtat tcttcctcct gcttcggtag atgggattct ctgcaccctg
    10141 tgaacaccac tctcaaattt caacctgctg taagcaccgc tgccttcaat ggaaaacaca
    10201 acctccttaa atcctcctat ttccgtggga ttggaatcaa gcatttctgt cttccacctg
    10261 tttctttcgg cgtaccgggt gtacattctg aaaagatccc ctgcaaaaag ggcagcctct
    10321 tcaccaccgg cacctcccct tatttcaaca attacgtttt tttcgtcatt ggggtcttta
    10381 ggaagaagga gtattttcat ttccttttta attacctcaa gcttttcctg ggcttccttg
    10441 agctcggttt ccaccatttc atgaaattct ctgtcaagct tctccttaag catctcctta
    10501 gcatcggaaa tttcctggct caccttttta tattcaccat acttggtaac aagctcctcc
    10561 aattccgcgt gttccttcat aagcttccgg tactcatcct gatttgcaat cacagccgga
    10621 tcgcttagct tgtggtttat ttcatcatat cggttctcag cagcctgaaa tctttcaaac
    10681 atattacacc tcttagtcca tcattaatta attttacgac catttattat accacgtatc
    10741 tgcattttat tctagggtgc attaacggag tgtgaaaaaa atacaggata taaactattt
    10801 acctaaatta tattaccatt atcaggattg attatctcct ttctttgtaa tattttctcg
    10861 gtataatagc ctatatcctg cttaattaat caaatcatcc cttaatgttg aacccgataa
    10921 accttatttt tttaatttca attctaataa gcaggcgaaa cacttattat ttccgggaat
    10981 aaaataacaa ttggaaccaa atattcgttt tctccaaaaa cattaaaagg attaaagaat
    11041 acttttctat tggtactgat tgtagcaaaa agaccagtct gcttagaaaa ttgaatagcc
    11101 gttgccatat cttttgtcac agatatgaat ggtgaattat ccgatgatat tgaatgtttt
    11161 gttgcaatat cttcataccc ggaattcggc attaaataac tcaaatacat ttgtgcgtaa
    11221 tcttctcccc ccatatttct aactgcgtta ctcaaaatac cgattccact attatattca
    11281 ctcac
//
LOCUS       DAOOJC010000016        11206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_5285_length_11206_cov_3.572594, whole genome shotgun sequence.
ACCESSION   DAOOJC010000016 DAOOJC010000000
VERSION     DAOOJC010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 11206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11206
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5285_length_11206_cov_3.572594"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..587)
                     /gene="greA"
                     /locus_tag="PLA01_00880"
     CDS             complement(111..587)
                     /gene="greA"
                     /locus_tag="PLA01_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPC99886.1"
                     /translation="MQNKEVVLTYEGLQKLEQELENLKTVRRREVAERIKQALSFGDL
                     SENSEYDEAKNEQAYVEGRIVQLENMLKNAKVIDEDEVKTDVVSIGSKVKVLDKEYDE
                     EVEYHIVGSTEADPSKYKISYESPVGKALIGKKKGTLVEVDVPDGTIKFKILEINK"
     gene            complement(870..1754)
                     /locus_tag="PLA01_00885"
     CDS             complement(870..1754)
                     /locus_tag="PLA01_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99887.1"
                     /translation="MKTKRIFSIDLPENSVKKITVDHDLSLFFIRLPYSLDELKKLRR
                     FTVRRIQKTVFKVCRDNSIKKCIYPQSTPDEVINGNSIKNPFSGNFIYASLLVNILRL
                     VSIDKGMDIREFNMAILDTDNRNLLYFFIWQLSPLAKFLTIITKEKESVQKEIEDVFD
                     ETGLAVRVTDDIASGLDGIDVFINIGNLGEFTLRKKVICNAMVINYGDMNNDRIEFTG
                     GAVNGINVGLPAKIGGLIDEEFLNYFSRNEIAEIVFVNKINIGTNIIKDFADYNTMKG
                     IPEQFEKEGYSISLLKTV"
     gene            complement(2043..3788)
                     /locus_tag="PLA01_00890"
     CDS             complement(2043..3788)
                     /locus_tag="PLA01_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HPC99888.1"
                     /translation="MKRTILAVLLFILVLSGLAVIPLGYYLYFKPNNDVVPAFSEGQL
                     FLVIEGEQVISGNKPKLLEGEILLPFEVVKEYFDPNIFWDEALEKVTITTKDRVIRMK
                     TDNLNAIVNNEPITLNIPVTKDNDVIYIPIEFLADFYGIEVSYIKDSNAIIIDYKNTI
                     RQIAEPIDPNAVVRKGRSIRHPIIKKLDMNAENIDKNTLRIFEEYDKWYKVRTWDGAI
                     GYIEKRFVVVKRMIVQELVQDGQGKTVWKPEKGKINLVWDMVYSRRENHSSVGELKGL
                     DVISPTWFQVKNENGELINRAYSKYVEWAHGEGYQVWALLSNDFTDTKMTSKLLNNTD
                     SRDNLIREILAYAALYDLDGINIDFENMYQDDRDVFTQFVREISPLLREQGLVVSVDV
                     NDIACYDKKALSEPVDYIMYMAYDQHWSSSPVAGSVAQVSWQEKILKRVLEVEGVPRE
                     KLLLGIPFYARLWKEKDGNLTSSALTMKGAKNNIIENNAKVVWDEESGQFYAEYKKDG
                     TLHRLWLEDHNSINLRTSLVHKYKLAGTCAWSLYFAIEDVWNVWDVLNRNLKEINGYH
                     EWTEQNTDRNYEFKP"
     gene            complement(3961..4482)
                     /locus_tag="PLA01_00895"
     CDS             complement(3961..4482)
                     /locus_tag="PLA01_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6106 family protein"
                     /protein_id="HPC99889.1"
                     /translation="MDIFIEKLVKKKRDTLDNVIVFGSIILAVVLMTLLILFLGPYAT
                     SVFLCIGIGYVAYLVIVSRRIEFEYALTNGQLEIDKIVNQKNRKNLINANCRDFEIMA
                     KVASEKLDEAVKGISDKIMAVSSLDSQDIYFFITIKKADEDEKAKRVLVLFEPDDKML
                     ETIKAMIPRKFFD"
     gene            complement(4729..6354)
                     /gene="groL"
                     /locus_tag="PLA01_00900"
     CDS             complement(4729..6354)
                     /gene="groL"
                     /locus_tag="PLA01_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814034.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPC99890.1"
                     /translation="MAKEIKFSEEARKALESGVNQLADTVKVTLGPKGRNVVLDKKFG
                     SPLITNDGVTIAKEIELEDEFENMGAQLLKEVATKTNDVAGDGTTTAILLAQAIIREG
                     LKNVAAGANPMILKKGISKAVDTAVEGIKEISQKVKGKEDIARVASISANDEVIGNLI
                     ADAMEKVTNDGVITVEESKTMGTNLEVVEGMQFDRGYVSPYMVTDTEKMEAVIDDPYI
                     LITDKKISNVQEVLPLLEQIVQQGKKLVVIAEDVEGEALATILVNKLRGTFTCVAVKA
                     PGFGDRRKAMLEDIAILTGGQVITSDLGLDLKDTTIEQLGRARQVKVQKENTIIVDGA
                     GSQEEIQKRIASIKAQIEETTSDFDKEKFQERLAKLSGGVAVIQVGAATETEMKEKKL
                     RIEDALAATKAAVEEGIVSGGGTALINTIPKVQKLLETVTGDEKTGVQIILRALEEPV
                     RQIATNAGLEGSVIVEKVKNSDKGMGFDALNEKYVNMIEVGIVDPAKVTRSALQNAAS
                     VASMLLTTEAVVGDIPEKEAAPMAAPGGMGGMY"
     gene            complement(6473..6757)
                     /locus_tag="PLA01_00905"
     CDS             complement(6473..6757)
                     /locus_tag="PLA01_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPC99891.1"
                     /translation="MNIRPLADRVVVKMVESEETTKSGIVLPGSAKERPQVAEVVAVG
                     PGTVVDGKEVKMEVKIGDKVIISKYSGTEVKFDGQEYTILKQGDILAVVE"
     gene            complement(7074..8204)
                     /locus_tag="PLA01_00910"
     CDS             complement(7074..8204)
                     /locus_tag="PLA01_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010680936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HPC99892.1"
                     /translation="MDEYKLRQLLKKEEGPKLDFKAELNLFTESDKKELTKDVIAMAN
                     SRGGRGYILFGIEDKTKRILGIDPANFSEEQIQQIIYNRCDPPVPISVDFFNIEGKMI
                     AAITVYKSNNKPHQMIQNGAFYIRRGSTTGVARRNEVANLLQENGLMSYETVILKNVG
                     LEELDYDLIKDYFKTLNVFSDNPSELILEALGIIGELLEGAGYHPTIGGLLLFGKNPF
                     LYLPHVYIKVVCKEDVRLFYGNILRMLDDVSEYMRSIIRAENYPFDALEEVIANALVH
                     RDYLDVSKGITITVTKKNIEISNPGALIANNSIYRFSRENNPDRRNSWLYQRLLTLDT
                     KRRFMKSGLGMKRVKNSFASIGLVKFINIGSQNLFKVLLPFA"
     gene            complement(8229..8891)
                     /gene="hisIE"
                     /locus_tag="PLA01_00915"
     CDS             complement(8229..8891)
                     /gene="hisIE"
                     /locus_tag="PLA01_00915"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HPC99893.1"
                     /translation="MENILEQIKFDDKGLIPAIAQDYKTNEVLMMAYMNEEALKKSLE
                     TGKAHYFSRSRNKLWLKGETSGHFQNIKSISVDCDGDTLLLKVEQVEAACHTGHYSCF
                     YRELSENELKETSQKVFDENKVYAAQKAKILQEVYDVIVDRTIHPKEGSYTNYLFEKG
                     IDKILKKVGEEAAEVIIASKNKEKGEVVYEISDLFYHLFVLMVERGVKLDDIYEELKK
                     RR"
     gene            complement(8910..9671)
                     /gene="hisF"
                     /locus_tag="PLA01_00920"
     CDS             complement(8910..9671)
                     /gene="hisF"
                     /locus_tag="PLA01_00920"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPC99894.1"
                     /translation="MLTKRIIPCLDVHNGRVVKGVNFVNIRDAGDPVEIAAYYDKAGA
                     DELTFLDITASADARNIMIDVVKKVAEQVFIPFTVGGGIRTVEDFREILKAGADKISV
                     NTAAIKRPELISEAASRFGSQCVVVAIDAKRRQYNSGWDVYINGGRDNTGKDVLEWAL
                     EVEKLGAGEILLTSMDCDGTKEGYDIELTRKVSESVRIPVIASGGAGTMEHFYDALVD
                     GKADAVLAASLFHFKEIEIMELKKYLKGRGIEVRM"
     gene            complement(9684..10397)
                     /gene="hisA"
                     /locus_tag="PLA01_00925"
     CDS             complement(9684..10397)
                     /gene="hisA"
                     /locus_tag="PLA01_00925"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HPC99895.1"
                     /translation="MIIYPAVDVKDGRCVRLAQGEFDRVTVYSDNPVEMALKWEQLGA
                     QYLHVVDLDGARTGHVQNIPTISEMAVKLGIPVQIGGGIRTIEMIETILCKGIHRVIL
                     GTSAVKDPNLVKNALKSFGDSIAIGIDAKDGMVAIEGWAKTSEFTAVGFAKKMEELGA
                     STIIYTDISRDGMLAGPNLKAMEEMVKSVGIEVIASGGVSKLEDIKNLKEVGVSGAIV
                     GKALYTKDLDLKEAIEIAK"
     gene            complement(10469..11080)
                     /gene="hisH"
                     /locus_tag="PLA01_00930"
     CDS             complement(10469..11080)
                     /gene="hisH"
                     /locus_tag="PLA01_00930"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPC99896.1"
                     /translation="MIAIIDYGMGNLRSVEKAFHFIGYEAEISSDKDFILNADAVVLP
                     GVGAFADAMYSLEKNGMIEVVRKVIDSGKPFLGICLGMQLLFDYSEEGGKNCNGIGIL
                     KGAIKQLPFGNLKVPHMGWNCLEISRQSPLFDGIRKKTYVYFVHSYYLDADDKDIVIA
                     STNYSINFAVAVQKGNVFATQFHPEKSGDVGLGMLRNFASYKL"
BASE COUNT         3169 a         2685 c         1623 g         3729 t
ORIGIN      
        1 tataattctt tgttacacaa ttactactca taaaataaca gtttacattc aatgtaaact
       61 gttatttaaa attatatatt aatttaaact tatattacaa ggtttttccc ctatttatta
      121 atttccagga ttttgaattt gatagtccca tcgggtacat cgacttcaac caaagtaccc
      181 ttctttttac ctatcaaggc cttgcccaca ggtgactcat aggaaatctt atatttactg
      241 gggtctgctt ctgttgaacc gactatatgg tactctactt cctcgtcata ctccttgtct
      301 aataccttaa ccttagatcc gatacttact acatctgttt taacttcgtc ttcatctatc
      361 actttagcat tcttaagcat attctccagt tgaactattc ttccttcaac atatgcctgc
      421 tcatttttag cttcatcata ttcggaattt tcagataaat ctccaaatga aagggcctgc
      481 ttgattctct ctgctacctc tcttcttctt acagtcttca agttttccag ttcttgttcc
      541 aacttttgca atccctcata tgttagaaca acttctttgt tttgcatttg aagcctctcc
      601 tttactttga atttcgtgtt gataatatat gcatttattt cttatttatt ctagattgaa
      661 acccactaaa aacacaaaca gcactgaaac ttcaaaacag tgctatggat aaactacaga
      721 gctaaacaga tgataataat atatgtgacc aactttataa ccaattcggc ataaatacta
      781 tagaacaacc caatttttta tcattataga ctactaatct tatattgtca ataaccaatg
      841 ataaaaaagg aaaaattgga ttgaacccgt catacagttt tcagtaatga aattgaatac
      901 ccttcctttt cgaactgctc cggtataccc ttcatagtat tgtaatcggc aaaatcttta
      961 ataatattag ttccaatgtt aattttattt acaaaaacga tttctgcaat ttcattcctg
     1021 ctaaaataat tcaagaactc ttcatcaatc aatcccccga tcttagcagg caaaccgaca
     1081 ttgatcccat taacagcccc acccgtaaat tcaattcgat cgttattcat atccccataa
     1141 ttaattacca ttgcattaca tataaccttt ttacggagag taaactcacc cagattacca
     1201 atgtttataa aaacatctat gccatccaaa ccactcgcaa tatcatctgt taccctcacc
     1261 gccaatcctg tctcatcaaa aacatcctct atctcctttt gaaccgattc cttctctttg
     1321 gttatgatag ttaagaactt tgctaaaggc gataactgcc agataaagaa atacaacagg
     1381 ttacggttgt ctgtatcaag tattgccatg ttgaattctc ttatgtccat acctttatca
     1441 atggatacca accttaaaat attaaccagc agcgatgcat aaataaaatt ccccgaaaag
     1501 gggtttttaa tactattgcc attgataacc tcgtccggtg tgctttgggg ataaatacac
     1561 tttttaattg aattgtcacg gcaaacctta aatactgttt tttgaatacg tctgacagta
     1621 aaacgcctta atttctttaa ctcatccaac gaatagggaa gccttataaa aaaaagactg
     1681 agatcatggt caaccgttat ctttttgact gaattttcag gtaagtctat actgaatatt
     1741 ctttttgttt tcatgcaaaa cagttttatt attcctgcta aaaatgcaaa aatattctta
     1801 ttttcctgtc tgtcattatc actaaaaact ataaggccta cattgttcat ctccgtatcc
     1861 catacctttt ataagttggc tatatactaa tcctatttga tacggaagct aaatattcat
     1921 aaatatataa tctcaaggat gcatgtaata aatgcaggga aaaatattcc agatgtttaa
     1981 gtttgttttt cataactttc tgtacctgat cagtgtgcat ccatattcat gagtgaagtt
     2041 tactatggtt taaactcata gtttctgtcc gtgttttgct cagtccactc atgatagccg
     2101 tttatctcct tgagattcct gttgagaaca tcccaaacat tccagacatc ctcaatcgca
     2161 aaatacaggc tccatgcaca agtacctgca agcttgtatt tatgcaccag agaggttctt
     2221 aagtttatcg aattatgatc ctccagccag agtctatgaa gtgtaccgtc ctttttgtat
     2281 tcggcataga actgtccgct ttcctcatcc cagaccactt tagcattatt ttcaattata
     2341 ttgttttttg cccccttcat tgtcaatgca ctgcttgtga gattcccgtc cttttccttc
     2401 cacagccgtg cataaaaggg aatgcctaaa agaagcttct ccctcggaac tccttccacc
     2461 tccagcactc ttttcaatat cttctcctgc caactaacct gtgccaccga tccggctaca
     2521 ggagatgaac tccagtgctg gtcatacgcc atatacatta tataatccac cggctcgctc
     2581 aaagcctttt tatcatagca ggcaatgtcg ttgacatcta cggataccac caatccctgt
     2641 tctcttaaaa gaggagatat ctctcttaca aactgtgtaa atacatccct atcatcctgg
     2701 tacatgtttt cgaaatcaat gtttatgccg tcaaggtcat agagagctgc ataagcaagt
     2761 atctctctga taaggttgtc ccttgaatca gtgttgttca agagcttgct tgtcatcttg
     2821 gtatctgtga agtcattact taaaagggcc cacacctggt aaccttcacc atgggcccat
     2881 tcaacatact ttgagtatgc tctgtttata agttccccat tctcattttt tacctgaaac
     2941 catgtgggcg aaatgacatc aagtcctttt aactccccta ccgagctgtg attctccctc
     3001 cttgagtaca ccatatccca tacaaggttt atcttgccct tttccggctt ccagacagtc
     3061 tttccctgac cgtcttgcac aagctcctga acaatcattc tttttacaac cacaaaccgt
     3121 ttctcgatat atccaatagc tccgtcccag gttctgacct tataccattt atcatactcc
     3181 tcaaaaattc ttaaagtgtt cttatcgata ttctccgcgt tcatatcgag ttttttgata
     3241 atcggatgcc ggatggaacg tccttttctt acaacagcat tggggtctat cggttcggca
     3301 atctgtctta tagtgttttt ataatcaata ataattgcat tgctgtcttt aatgtatgat
     3361 acctcaatac cgtaaaaatc ggccaaaaac tctataggta tatatataac atcattatcc
     3421 tttgtcacag gtatgttcag agttataggc tcattgttca caatcgcatt aaggttatcc
     3481 gtcttcatcc ttatgactct gtccttggta gtaatggtaa ccttctccaa agcctcatcc
     3541 caaaaaatat ttgggtcaaa atactcttta accacttcaa acggaagaag tatctcccct
     3601 tccaaaagtt tgggcttatt cccggatatc acctgctcgc cctcgattac caaaaaaagc
     3661 tgtccttcac taaaagcagg aaccacatca ttattcggct taaaataaag ataatacccc
     3721 agaggaataa ctgccaaacc tgatagaaca agtataaata ataaaacagc aagaattgtc
     3781 ctcttcattt tgacccccta taaattagtc tcaatcatat aacattatac aacaaaattc
     3841 gtggtaattc accaaataaa taaaggaaga ggcgtaggat ttctaattac aaaatattcc
     3901 taatgaatta tccatcaaca atatttcatt attaaaatta tctagaaaca atctatttaa
     3961 ctaatcaaaa aactttcttg gaatcattgc tttgattgtc tcaagcattt tatcatcagg
     4021 ctcaaaaagt actaaaactc tctttgcttt ctcatcctca tcggcttttt taattgtaat
     4081 aaaaaaataa atatcctgag aatccaatga acttacagcc attattttgt ccgaaattcc
     4141 ttttactgct tcgtcaagct tctcggaggc taccttggcc ataatctcaa aatccctaca
     4201 gttcgcattt ataagattct ttctgttttt ctgatttaca attttatcta tttccaactg
     4261 cccgtttgtg agtgcatatt caaattctat tcttctcgac actattacaa gataagcaac
     4321 atacccgatt ccaatacaca gaaatacaga agtggcataa ggccctaaaa aaagaattaa
     4381 cagcgtcatt aacacaaccg caagaattat tgagccaaac actataacat tatctaaagt
     4441 atccctcttc tttttaacca gcttctcaat aaaaatatcc atcatttacc tcactcaatg
     4501 taaaactctt aatactgtaa attatataat atataaatat tttgtacaaa tcaaggcata
     4561 ttccagagtg cattaaataa atgtcaagaa gaatatgcca tattctaata ctataaaaaa
     4621 acagatagac ggcgtagtct cataactaca ccgtctaatg aaaaacttaa acttcctttt
     4681 tgcacacccg attttttagg gcttttcttt tcatgaatcg aatttaaatt agtacattcc
     4741 gcccattcca cccggagcag ccataggtgc agcttctttt tccggaatat cgccaacaac
     4801 agcttcagtc gtaagcaaca ttgatgcaac cgaagcagca ttttggagtg ctgaccttgt
     4861 aacctttgca gggtctacaa taccaacttc aatcatgttg acatactttt cgttcagtgc
     4921 atcaaaaccc atgcccttat cgctgttctt aaccttttca actattacag aaccttcaag
     4981 accggcattg gttgcgatct gtctaaccgg ctcttccaaa gctctcaaga ttatctgaac
     5041 accggttttc tcgtccccgg ttacagtttc aagaagcttt tgaacttttg gaattgtgtt
     5101 tattaaagca gtacctccac cggatactat tccttcttca acagcagcct ttgttgcagc
     5161 aagggcatcc tctattctca atttcttttc tttcatttca gtttcggtag cagcaccaac
     5221 ctgaataact gctacgccgc cggagagctt agcaagtctc tcttgaaatt tttctttgtc
     5281 gaaatccgac gtggtttctt cgatctgggc ttttattgat gcaattctct tttgaatctc
     5341 ttcctgactt ccggcgccgt caactataat ggtattttcc ttctgaacct tcacctgtct
     5401 tgccctacct aactgttcta tagtagtatc cttcaaatcc aggccaagat ccgatgttat
     5461 aacctgcccg ccggtaagaa ttgcgatatc ctcaagcata gcctttcttc tgtcaccaaa
     5521 gcccggtgcc tttacagcta cgcatgtaaa tgttcctctc aacttgttta caagtattgt
     5581 tgctaaagct tcgccctcaa catcctctgc tataacaacc agttttttgc cctgttgtac
     5641 tatttgttca aggagcggga gaacttcctg cacattgctt atctttttat cggtgatcaa
     5701 gatatatgga tcgtcgataa ctgcttccat cttctcagta tcagtaacca tgtatggtga
     5761 tacatagccc ctgtcaaact gcataccttc aaccacttca aggtttgtac ccatggtttt
     5821 tgactcttca acggtaataa ccccgtcatt tgtaaccttt tccattgcat cggctatcaa
     5881 gtttccaata acctcatcat tggcggaaat tgatgcaacc cttgctatat cctcttttcc
     5941 ctttaccttc tggctgattt ccttaatacc ttcaacagct gtatcaactg ctttggatat
     6001 accttttttc aggatcatcg gatttgcacc agctgcaaca tttttaagtc cttccctgat
     6061 aattgcctga gcaagaagaa ttgcagttgt ggtaccgtca ccggcaacgt cattggtttt
     6121 agttgcaact tctttaagaa gctgtgcacc catgttttca aattcatctt cgagctcaat
     6181 ttcctttgca attgttacac catcgtttgt gattaacggt gaaccgaatt tcttatccaa
     6241 cacaacattc ctgcccttag gaccaagagt aacctttact gtatccgcta actgattaac
     6301 accgctttcc agggcttttc ttgcctcttc actgaacttt atttcctttg ccatataata
     6361 acctccttta ggtatacttt tttcggacaa tcataaaata agtacaaaaa taagtaagaa
     6421 ttataaagtc gttaccttac tcaaaattaa atccttgagt ggcaccgaca tattactcaa
     6481 ctaccgcaag tatgtctccc tgctttaaaa ttgtatactc ctgaccgtca aatttgactt
     6541 ccgtaccgga atatttgctt attataacct tatctccaat tttcacttcc attttaactt
     6601 cttttccatc tacaacagta cccggtccaa ctgcaacaac ttcagccacc tgtggtcttt
     6661 ctttagctga gcccggcaaa acgattccac tctttgtggt ttcttcactt tctaccattt
     6721 taacaacaac tcggtccgct aatggtctaa tattcatata aaaacctcct ctttaatatt
     6781 tatttaaaat atatgtccgt ttattattag cactcaccga aagtgagtgc taatcgcaaa
     6841 tataatatta ttatattacc cttttttata tcaaacatgt attttttcta tattttattg
     6901 ttttgagaga atattcggag aagagcgtaa aatacgttaa tatttcattg attttcttta
     6961 gttttcgtca aagcaaacgt atttacctgt ttacattaag taaatgcgaa aaaaactcgc
     7021 cataaacttg agtttcttgt gcatcttctt tggcacagat aactttatta tgtttacgca
     7081 aagggcaaca gcaccttgaa tagattctgg gacccaatat ttatgaattt gaccagtccg
     7141 atggatgcga aggaattttt cactctcttc attcccaatc cggatttcat aaaccttctt
     7201 tttgtgtcta atgtcaaaag cctttgataa agccatgaat ttcttctgtc aggattattc
     7261 tctcttgaaa acctgtaaat gctgttatta gctataaggg caccgggatt gctgatctca
     7321 atgttctttt ttgtaacggt aattgttatt ccctttgaaa catcaaggta atccctgtga
     7381 accagagcat tggcaataac ctcttccaat gcatcgaagg gataattctc agcccttata
     7441 atacttctca tatactctga cacatcgtca agcattctta gtatattccc gtaaaacagc
     7501 cgcacatctt ctttgcaaac caccttgata tacacatggg gaagatataa aaaaggattt
     7561 ttcccgaaaa gcaacaaccc ccctatggtt gggtgatacc ccgctccctc aagcagttca
     7621 ccgattattc ccagtgcctc aagaatcagc tcactgggat tatcgctgaa cacattcagt
     7681 gttttaaaat aatcctttat aaggtcataa tccaattctt ccaaacccac atttttcagt
     7741 atcacagtct cataagacat aagaccgttt tcctggagga gatttgcaac ctcatttctt
     7801 ctggctactc cggttgttga accccttctt atataaaaag ccccgttttg gatcatctga
     7861 tgaggcttgt tattgctttt gtaaacagtg atggcagcaa tcatctttcc ctcaatatta
     7921 aaaaaatcaa cggatatggg gacaggaggg tcacaccggt tgtatataat ctgctgaatt
     7981 tgctcttccg aaaaattagc aggatcaatt cctaatattc tctttgtttt atcttcaatg
     8041 ccaaaaagaa tatagccccg cccacctctg gaattggcca tggcaattac atccttggtg
     8101 agttccttct tatcactctc agtgaataaa ttaagctcag ctttaaaatc aagtttcggt
     8161 ccctcttcct ttttcagcaa ctgcctcagc ttatactcat ccattgactt ttacctccgg
     8221 aatagcctct atcttctctt tttcagttct tcatatatat catccagctt aactcctctt
     8281 tccaccatta aaacaaagag gtgataaaag aggtcggata tttcataaac tacttcgccc
     8341 ttttctttat tctttgatgc tataataacc tctgcggcct cttctccgac tttttttaat
     8401 attttgtcaa tgcccttttc aaacaagtaa ttggtataag agccttcctt gggatgtatg
     8461 gttctgtcga ctattacatc atatacttcc tgaagtattt tagccttttg agcggcataa
     8521 accttatttt catcaaatac cttttgcgat gtttccttta attcgttttc ggaaagctct
     8581 ctgtaaaaac aggagtagtg accagtatga caggcagctt ccacctgctc aacctttaaa
     8641 aggagagtat caccgtcaca gtctacactg atggatttta tattctggaa atgaccggag
     8701 gtctctccct taagccacag cttgtttctg ctcctgctaa aataatgagc cttccccgtc
     8761 tctagcgatt ttttcaaggc ttcttcattc atataagcca tcatgagtac ttcattggtc
     8821 ttataatcct gtgcgatagc agggattaat cccttatcgt caaattttat ttgttctaat
     8881 atattttcca tttttattta cccccaaacc tacattctta cttctatacc ccggcctttt
     8941 aaatattttt taagttccat tatttctatt tctttaaagt gaaataggga tgcggccaat
     9001 acagcatcag cctttccgtc caccagagca tcatagaaat gctccatcgt acccgcaccg
     9061 ccggaagcaa tgactggaat cctcacgctt tcggatacct tccgggtaag ttcaatgtcg
     9121 tacccttcct ttgttccgtc gcaatccatg ctggtaagaa gaatttcacc ggcacccagc
     9181 ttttccacct ctaaagccca ttccaaaacg tcttttcccg tattgtccct tccgccgtta
     9241 atgtatacat cccaaccgga gttatactgc cgtctttttg catcgattgc caccacaaca
     9301 cactggcttc cgaaacgtga tgccgcttcc gatataagtt ccggtctttt tattgcagcg
     9361 gtgtttacgg atattttatc cgccccggcc ttaagtattt ctctgaagtc ctcaacagtt
     9421 cttatccctc cacccaccgt aaaaggaata aatacctgct ctgcaacctt tttaaccaca
     9481 tcaatcatga tattccttgc atctgccgaa gctgttatgt ccagaaaagt cagctcatct
     9541 gccccggcct tatcataata tgcagcgatt tcaacagggt caccggcatc tcttatattg
     9601 acaaaattta ctcccttaac aactcttcca ttatgaacat ccagacatgg aataattcgt
     9661 tttgtcagca tttttaatcc cccttatttt gctatttcaa ttgcttcctt taaatccagg
     9721 tctttcgtgt aaagtgcttt acccacaata gctccggaaa caccaacttc cttcagattt
     9781 ttaatatcct caagcttgct tactccaccg gaagctatta cttctattcc gacagacttc
     9841 accatttctt ccatagcctt aaggtttgga cctgcaagca ttccatccct tgaaatgtcc
     9901 gtatatatta tggtacttgc ccccagctcc tccatctttt tggcaaatcc tactgccgta
     9961 aactcactgg ttttagccca accttctatt gccaccattc cgtctttcgc atctatccct
    10021 atagcaatac tgtctccaaa actcttcaat gcatttttta ccaaattggg atccttcacc
    10081 gctgaagttc ccaatattac acggtgaatg cccttgcata aaatggtttc aatcatttct
    10141 atagtcctta ttcctccacc gatttgtacc ggaataccta gctttacagc catctcactt
    10201 atagtgggga tattctgtac atgaccggtt ctggctccgt ccaaatcaac cacgtgcaga
    10261 tactgggcac ccaactgttc ccacttcagt gccatttcca ccggattgtc agaataaacg
    10321 gtaaccctgt cgaactcacc ttgtgcaagt ctgacacatc ttccatcctt aacatccaca
    10381 gccggataaa taatcataca aagcttacct cctgtgtaca cttaaatttt tccatgctat
    10441 gaagtagatt gcttcccttc atgctttttt ataatttata agacgcaaaa tttctcaaca
    10501 taccaagccc cacatcaccg cttttttccg gatgaaactg ggtggcaaaa acattaccct
    10561 tttgtaccgc aacagcaaaa ttgatgctgt agttggttga agcaataact atatctttat
    10621 catcggcatc aagataataa gaatgaacaa agtaaacata ggtttttttg cgaataccgt
    10681 caaacaacgg tgactgacgc gatatttcga gacagttcca ccccatgtgg ggaaccttaa
    10741 gattaccgaa gggaagctgc tttattgccc ccttaagtat cccgatacca ttgcagtttt
    10801 ttccgccttc ctcgctgtaa tcaaataaaa gctgcattcc aaggcagata cccaaaaagg
    10861 gtttaccgct gtcaataacc tttctcacca cttcaatcat gccgtttttt tcaagactgt
    10921 acatggcatc ggcaaaagca ccgacacccg gtagcaccac agcatctgca tttaatatga
    10981 aatctttgtc cgaagatatt tctgcttcat aaccaataaa atgaaaagct ttttcaacgc
    11041 ttcttaaatt tcccattcca taatctatta ttgcaatcaa acttgttcca ctccacaatt
    11101 aaatttaatc atgcaaacaa ctaaaactcc cctttaccga ataggtatac ggggagtgct
    11161 gttgtttaat ccagtttctt gcctgtcagg cgttcatatg cttcaa
//
LOCUS       DAOOJC010000017        11089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_5358_length_11089_cov_4.081385, whole genome shotgun sequence.
ACCESSION   DAOOJC010000017 DAOOJC010000000
VERSION     DAOOJC010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 11089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11089
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5358_length_11089_cov_4.081385"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PLA01_00935"
     CDS             complement(<1..965)
                     /locus_tag="PLA01_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPC99897.1"
                     /translation="MKCNFDFEDIIKYTENQLSDEEKKRIKEHLDTCEKCRRHYVVLK
                     YTESYAKDSSLASKSISKNVMEAIDMNKYSKNKKSWAGKAFYKAMHVIKPIAATAAVL
                     LLAIVLVANFDNLRGLINNTSSGGPNPSASAVNPQNTNTAVLPENTKPADQDSVRKRT
                     ITLYFANTDADKVVAEKREVEISQNTQIEKLVFEELQEGPKSEDLHSAIPEGTRLLSA
                     STENSICTLNLSREFVDNHPGGSAGESMTLHSIINTMTELPGIDKVQFLIEGQKQEAY
                     IHAVFNEPFERNEEVIGKRPSEIKTEVEARSQAAILTIKEKDMEKP"
     gene            complement(968..1549)
                     /locus_tag="PLA01_00940"
     CDS             complement(968..1549)
                     /locus_tag="PLA01_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPC99898.1"
                     /translation="MIGVLTMKKERDNMLVESTLKGNIDSFEELITLYQDKLFNFLSK
                     MTYSREDAEEITQEVFIKVYNNLYKYNNKWSFSTWLYRIAINTLRSEYRKVNNYKKID
                     YYTNVPDLPANFSDYPEIAYEIKEQRGEIIKLIDDLKEDQKTALLLRCIQDFSFKEIG
                     DILGISPEAAKMKIQRAKQTICRKYEKLEKGGK"
     gene            complement(1808..3085)
                     /locus_tag="PLA01_00945"
     CDS             complement(1808..3085)
                     /locus_tag="PLA01_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HPC99899.1"
                     /translation="MIETTIAFSRYFTVLLFGTAIALSFAGMARTRKNYMAAGCLILV
                     LFVVQVISLQTWGMAATIKLYPLISHLPIAVFIVVYLKRPWLISLTAVFASFLCCQPP
                     RWIGTALGEVLDNVSINHVGYIVSAFLIYYFLQKFAVESVRHLMERSVSSCVLFCAMP
                     AFYYIFDYTATVYTDFMYSGARVAVQFMSFITSTFYFVFVLLYYAETQKQASIQRERD
                     MLDTQFRQAQTEFESLRQMQQNAAAYRHDMRHHFSLLQRMASKENIEEIKEYLKTVQS
                     DIDSITPVRYCENETVNLILSSFAAKAKQSKILLTIDANLPDAYPFSNTELCSLLSNA
                     LENAIQASEQITDSSKRIIQLRIFSKNNKLCIDIRNRYQIEPVFRHGLPVSTKQEQGH
                     GFGTKSMAHIVEKNGGVFRFSVKDGWFIFQATT"
     gene            complement(3090..3815)
                     /locus_tag="PLA01_00950"
     CDS             complement(3090..3815)
                     /locus_tag="PLA01_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPC99900.1"
                     /translation="MLQIVLCDDNINELSYMVQLLKQYSSSKHLDCEYTLFHNGFELI
                     SALDKGKRFDIYCLDIMMPGFTGIDAANEIRSFDKTAPILFFTSSPEFALESYSVKAV
                     NYVLKPITKEKFYSTFDDLLEQIKVKKEEDAIVVKSNDGIQRVLISNIVFAEVIGRNV
                     LYHLISGRTIECTESFSTVCNNLLRYGCFLKTHRSYLVNMQYIDTVDNHQVTLQTLDT
                     VPIAQGKAKEIKQQYLNYQMEEE"
     gene            4267..>5498
                     /locus_tag="PLA01_00955"
     CDS             4267..>5498
                     /locus_tag="PLA01_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPC99901.1"
                     /translation="MKKRFISIFLLVAMLFTLFPAELMAVMTDNLNTSVNIVQEKTRH
                     ENPFVDVSEGNWYYDAVQYSLLNGFFSGTSQNTFNPDGTMTRGMFVTVLGQMAGVDQS
                     AYKGLSAFSDVAQDAYYAPYVAWASKHGITAGVGNGKFDPNGLVTREQMAVFFVCYFE
                     KFDVNFDTGANITTVPQDLDTISPWAREKVLKLWKTGLLAGDGTNFNPKGNANRAQAA
                     ALCMTADKAVKTWYSEPGIPSERVSVDPNDGQNPETPDTSTGDSDTESNTEDSDSEDS
                     EGDSDTGKNTGGSKNNSKTTYYKVTFVIDSKREEKLYKKDTLLSTMPIPAQPSGKVFL
                     GWYYDEEKTKPVSGGDKLNSDITLYANFTEAVKLDESGTLNFVSVLDQEPEFTITVKK
                     TGAPPILGTDFKFRNITAP"
     assembly_gap    5499..5508
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5541..10334
                     /locus_tag="PLA01_00960"
     CDS             5541..10334
                     /locus_tag="PLA01_00960"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HPC99902.1"
                     /translation="MANIETVEVEGSNGVWTIKSANGGFTPGHTYQIELINDAVIYDD
                     SADAFGNIKDNNELYDITQVRFFNFSIAQDGTMNLKLKAGIKYINAENISEADGANLM
                     EYAGLYLAGTDDQGNITYTANNGSGSFTYDGGDIQVGDVVSIYSGTIPTNRKPEKGTD
                     DKTYNGDVAYMKIINIDGSTHYYAAAEAEDVLFTPDVLPINVEEGKGTTGWTDNGTTV
                     TVVSSVLDFSSSEYEDMGLNSETTVDVGDFLVFYIGNFGEINAQDITYGEVTKIEVNE
                     DSTTTINYNPVEEKDILSAMELYDETKLPKDEILDSINKNKEEIQEIIEAQLMGSNFF
                     DEAGEYLAELALQTDEVREVFGDDLTLSDCTITYADGTPLGANELTLMGNIIDKDQDG
                     KKPKVSVSVSTLTTHFDPKLVGSGIRAEVAVSYKFKIQKKGNNNIMEVELTAFFEQEF
                     TFGFNVSGGAVWKWKWKIIPYIDDYRMTGNLDIGTYTGLGITTTAKLTQAKEEWGMPW
                     PNSAKEAKATGKIFSLSKSIKELVESVRTVLPEEEATGSGGLAEKYARFMEDANEEWV
                     DLVSVNLINLRSAVDPLHILAFGLDVDFVVSANLNVAIGMTFQYENFKRHSFTLLLFS
                     KKSESESIDLSTNGYQFDFYVMGTVGIRAGVRVKVTMGLFSTKLAAIGLQFEAGAYVR
                     LWGYFYYHLENWKVDGKWQKESGYSGALLIEVGAYLDIKFIAEALNGKYSYTPSIYVK
                     EWPLWSAGQRENVYDFAYEDNLTFDILNVSTYTLPKTVFDMYWMDIKTGELEEGFNNK
                     IKNFDSNKAPGGDDEEYFAVELSNRNFSYNPVNNQITVNTDSGEVAQSCEMNITWKGT
                     SLSGLSEVLSRTVKLNWSNEKNAGTIAFESNGGSVVPMVRLLTGTPLTGRMPAPPVRL
                     GYTFAGWYEDADFSTIFDAANMPAGNKTLYAKWTPNDVSYIVEHYQKALNGQYVLVKT
                     DKDQKGKVGEQTAAVAETYTGFTAQPVKQQTIVSDGSTRVTVYYDRNLYDLKFVYGNG
                     SNDISLKVAYGSDIVKPSNPLKAGYSLDGWDADIPSTMPANNLTFTARWTERTDTPYK
                     VKHYKQNLDESYKLEQTDEKTGTTGQLTDAVTKTYTGFTAKTFAQKTILPDGSTVVEI
                     YYDRDSFALSWNVNGGKPLTGSYTYGSVKYGTPIKKPDMPTKTGHTFAGWYMDEELTA
                     ALEDNATMPAKDITMYASWEVEQYTISFDSNGGSPVPPITQEYGTAVSEPAAPVRAGY
                     NFTGWKLNGENYTFTTMPGENITLIAQWTAHTNIPYTVEHYFEKVDGSYPSIPDETEN
                     LTGTTDSTVTANAKDYTGFTFDSGVSGTVTSGTISADGSLVLKMYYTRNSYTVTWDGN
                     GGNVDTNGATTGSVKYGADITSPANDPVRTGYTFNQWSGYTENMTMPAENITFTAEWT
                     INQYTITFDDISTEEDVQGMFITSRLIVNGTGTLTVTSGTGGAKYNCGIRVGSRGNDF
                     RINGLEVKAFAGAAGSGGKSYGVFMDSSIGTYVVQLTKGTLETHGYDCAGYASLSTAE
                     YFIINQYQQKFATITVSENYDGTDAIDVEQNATYQNYKYMYVTNP"
     gene            10502..>11089
                     /locus_tag="PLA01_00965"
     CDS             10502..>11089
                     /locus_tag="PLA01_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPC99903.1"
                     /translation="MKLHLFLAAILMSIFLTVPAQAEEAELVRIAIIDTGISTAAISE
                     RNLAKGRNYILSNATTEDTMGHGTAVAAIIAGSETAGVTGLYPEAVLVPLVYYSKNED
                     NTAFKGDLPMLAQIIRDAVDVYGCRIINISSGARVDTPTLRDAVAWAEQRGVLVVSCA
                     GNDGDGTAYYPGAFSTVLCAGTVNPEEDGPASFSNR"
BASE COUNT         3221 a         2226 c         2499 g         3133 t
ORIGIN      
        1 ggcttttcca tatccttttc cttaatagtc agtattgctg cttgagacct ggcttcaacc
       61 tcagttttta tctcgcttgg tcttttcccg atgacttctt catttctttc aaagggttca
      121 ttaaacacag catgaatata tgcctcttgc ttttgtcctt caataagaaa ctgtaccttg
      181 tcaatgccgg gaagttccgt catggtattg attattgagt gtagtgtcat tgactctcct
      241 gccgatcctc cggggtgatt gtcgacaaac tctcttgaaa gattaagggt gcagatactg
      301 ttctccgttg atgccgacaa aagccttgtt ccctcaggta tggctgaatg taagtcttca
      361 ctttttggcc cctcttgcaa ttcttcaaat accagttttt ctatttgtgt gttttggctt
      421 atttcaactt cccttttctc ggcaacaact ttgtctgcat cggtatttgc aaaataaagg
      481 gtaattgttc tctttcgcac tgaatcttgg tctgccggtt ttgtattctc cggtaaaacg
      541 gctgtattgg tattttgcgg gttaacagct gaagcggatg gattaggtcc cccactgctt
      601 gtgttattga ttaatcctct taagttatca aaatttgcaa caagtactat tgccaaaaga
      661 agtactgctg cagtcgccgc aataggcttt ataacatgca ttgccttata aaaagcttta
      721 cctgcccaag acttcttatt cttgctgtat ttattcatat caatggcctc cattacattt
      781 ttactgatgc tcttactcgc caaagaactg tcctttgcat aggattcagt atatttcagc
      841 acgacatagt gtcttctgca tttttcacat gtgtccagat gttctttgat tctctttttc
      901 tcttcatccg acagctgatt ttcagtgtat ttaattatgt cttcaaaatc aaaattgcac
      961 ttcattttta cttacccccc ttttccagtt tttcatactt cctgcatatt gtctgcttgg
     1021 ctctttgtat tttcatcttt gcagcttcgg gcgaaattcc taaaatatct ccgatttctt
     1081 taaaggaaaa atcctgtatg catcgtaaaa gcaaggctgt tttctggtcc tctttaagat
     1141 cgtcaatcag tttgattatt tctccacgct gctcctttat ttcgtaagct atctcaggat
     1201 agtcactaaa gtttgcagga aggtccggta cgtttgtgta atagtcgatt tttttataat
     1261 tattcacttt tctgtactca ctcctaagag tatttatggc tatacggtaa agccaggtgg
     1321 aaaaactcca tttattgttg tatttataaa ggttgttata tacctttata aacacctcct
     1381 gggttatctc ttctgcgtct tcccgcgagt atgtcatctt tgaaagaaag ttgaataatt
     1441 tgtcctgata taatgttatc aactcttcaa aggaatcaat attgcctttt aaagtacttt
     1501 caaccaacat gttgtcccgt tcttttttca tagttaacac accaatcata tgctattctc
     1561 ctcaaaaagc ttgttgttac gtcgataaat cttgttccga aaaacctgtt tatttgggta
     1621 taagctgctt ttcgaaaaga ttttgataat tattgaatcg tacgactcgc atcttttatg
     1681 agatgcttta tattattata ctcattaaat tgccggcagt aacggtaaaa atatattatt
     1741 tttttcgaaa aaaaaataat atgcacccgt ttttgaatag attacgaaag actaatagat
     1801 gaagaggtca tgtagtagct tgaaatataa accagccatc cttgaccgaa aaccggaaaa
     1861 caccgccgtt cttttcgacg atatgggcca tgctttttgt gccgaagccg tgcccttgct
     1921 cttgctttgt tgatacaggg aggccatgac gaaaaaccgg ttcaatttga tagcggttgc
     1981 gaatgtcaat gcacagcttg ttattcttgg aaaagatacg cagctgtatg atgcgtttac
     2041 tgctgtcagt tatttgttcc gaggcttgta tggcattttc caaagcattt gacaagaggg
     2101 aacaaagctc ggtattactg aagggatatg catcaggtaa atttgcatct atggtcaaaa
     2161 ggatttttga ttgttttgct ttggcggcga aagaggataa aatcagattg acggtttcgt
     2221 tttcacaata acggacaggt gtgatggaat ctatgtcaga ctggacagtc tttagatatt
     2281 ctttgatttc ttctatattt tccttggatg ccatcctctg caaaagagag aaatgatgac
     2341 gcatgtcgtg ccggtaagct gcggcattct gctgcatctg ccgtaacgat tcaaactctg
     2401 tttgtgcctg cctgaactgc gtatccaaca tatcccgttc tctttggata gatgcctgtt
     2461 tttgcgtttc ggcataatac aacaggacga acacaaaata gaaggtggat gttatgaagg
     2521 acataaactg tacagctacc cgtgctccac tgtacataaa atcggtgtac actgtggcag
     2581 tgtagtcgaa aatatagtaa aaagctggca ttgcacaaaa aagcacacaa gaactcacag
     2641 agcgttccat aagatgacga acggattcca cggcaaattt ttgtaggaaa tagtatatca
     2701 aaaaagcaga aacaatataa cctacatggt ttatggaaac attgtcaaga acttcgccta
     2761 gagctgtacc gatccagcgg ggaggctggc agcaaagaaa ggatgcaaac acagcagtca
     2821 gggaaatcag ccatggacgc ttcaaatata ctacgataaa aacagcaatg ggtaaatgtg
     2881 aaatcagcgg atataattta attgttgcag ccatacccca tgtttgcaaa gaaatgacct
     2941 gcacaacgaa aagtaccaag atcaagcatc cggctgccat ataatttttc cgtgtacgtg
     3001 ccattccggc gaaacttagt gctatcgctg taccgaacag caatacggta aaataacggg
     3061 agaatgcaat ggtagtttct atcatccgtt tattcttcct ccatttgata gttaagatac
     3121 tgctgcttga tttcctttgc ttttccttgg gcaataggaa cagtatcaag ggtttgcaag
     3181 gttacctgat gattgtctac ggtatctata tactgcatat ttacaaggta ggagcggtgg
     3241 gttttaagaa aacatccgta tcttaaaaga ttattgcaga cggtggaaaa ggactcggtg
     3301 cattctatcg tccttccgga aattaagtga tacaacacat ttcttccaat cacttctgca
     3361 aaaactatat ttgagattaa tactctttga atgccgtcat tactctttac cacaattgca
     3421 tcctcttctt ttttcacttt tatttgttcc agaaggtcgt caaaggtgga ataaaatttc
     3481 tccttagtga ttggcttcag cacatagttg acggctttca cggaataact ttccaaggca
     3541 aattccggtg atgaggtaaa aaataaaatc ggagcggttt tgtcaaaact gcggatttca
     3601 tttgccgcat caatgcccgt aaagcccggc atcataatgt ccaggcaata tatatcaaac
     3661 ctctttcctt tgtctaaggc tgaaatcagc tcaaatccgt tgtgaaaaag tgtgtattcg
     3721 caatccaaat gctttgatga actatattgc tttaatagct gtaccatata ggacagctcg
     3781 tttatattgt cgtcgcacag aacaatctgc agcaaattta ttccttcctt tctttatcat
     3841 ggttttaata cagagattgg atatcttgta ttatttcatg tcgaaattaa tatgattcat
     3901 gcaaaagtta tttttatacc gaaaataatt gatacattta atttaatgaa aaatatcttt
     3961 atgcaaatta acatatagta tttcggcaat atgtcaaggg ggctttaaaa gacaaaaaat
     4021 tatgtaaacc ttgatttttg tgcataagag catagattat tatgtatcaa cttgccgggc
     4081 ggagaaaagt cttcgccctt tcttgctttg acatggtaat ggctataaaa atgtttcctg
     4141 tggttatgat tgcaagggtt ttaggcttcg gcaaagtgac tgtctaaagg agcaggcttc
     4201 gttttgtgtg gtgtaactgt actttgtaat gcagtcagag aatactaact ggaaaggatt
     4261 gatgaaatga aaaagcggtt tatatccatt ttccttctgg tagccatgtt atttactctg
     4321 tttcccgcag agctaatggc tgttatgacc gataacttga atacatcggt caatatagtt
     4381 caggagaaaa caaggcacga aaatcccttt gtggatgtta gtgagggcaa ctggtattat
     4441 gatgcggtac agtattcact cctcaacgga ttcttcagcg gtacaagtca aaataccttt
     4501 aaccctgatg gcacgatgac ccgcggtatg tttgtcactg tactggggca aatggccggt
     4561 gttgatcaga gtgcatacaa ggggctgtcc gccttcagtg atgtggctca agatgcttac
     4621 tacgctcctt atgtagcgtg ggcatcaaag cacggcatta ccgccggtgt cggaaacggt
     4681 aaatttgacc cgaacggtct tgttacccgg gagcagatgg ccgtgttttt tgtgtgttat
     4741 ttcgaaaagt ttgatgtgaa ctttgatact ggagctaata taacaacagt tccacaggat
     4801 ttggacacca tatcgccatg ggcaagagag aaggttttaa agctttggaa aacaggactt
     4861 ttggccggtg acggcacaaa ctttaaccct aaaggaaatg caaaccgtgc ccaggctgct
     4921 gcactttgta tgactgcgga taaggcagta aagacatggt acagtgaacc cggtatcccg
     4981 tccgaaagag tcagtgtgga tccaaacgac gggcagaatc ctgagacacc ggatacttcc
     5041 actggtgatt ccgacacgga aagcaatact gaggattctg acagcgaaga cagtgaggga
     5101 gattccgaca caggaaagaa cacgggaggt tctaaaaaca acagcaagac cacttattat
     5161 aaagtaacct ttgtgattga ttcaaaaagg gaagagaagc tatataaaaa ggataccctg
     5221 ctaagcacca tgccaattcc ggcccaacct tccggaaagg tgtttttagg ctggtattat
     5281 gatgaagaaa agaccaaacc ggtatccggc ggtgacaagt taaattccga cataacgcta
     5341 tatgcaaatt tcactgaagc tgtcaaactg gacgagagcg gtacactcaa ctttgtcagt
     5401 gtattggatc aagagccgga atttactata acagttaaga aaactggagc tcctccgatt
     5461 ttaggtactg acttcaaatt ccgtaatatt actgctccnn nnnnnnnnaa acaccggaaa
     5521 cagaggttgg ggaagaggac atggcaaaca ttgagactgt ggaggtcgaa ggcagtaatg
     5581 gagtatggac aattaaatcg gccaacggag gattcacccc cggtcataca tatcaaattg
     5641 agcttatcaa tgatgctgtg atttatgatg acagtgcgga tgcttttggg aatattaaag
     5701 acaataatga actatatgat attactcagg tacgtttctt taacttcagc attgctcaag
     5761 acggaaccat gaatctaaag ctaaaagccg gcatcaagta tattaatgct gagaatatca
     5821 gtgaagcaga cggtgcgaat ttgatggagt atgccggttt gtatcttgct ggcacagatg
     5881 atcagggaaa cattacctat accgcaaata acggaagcgg cagctttaca tacgatggag
     5941 gcgatattca ggtgggtgac gttgtctcca tctattcggg aacaataccc acaaaccgta
     6001 aaccggaaaa aggaacggat gacaaaacct ataacggtga tgtcgcttat atgaaaatca
     6061 tcaacatcga tggcagtacc cactattatg cagcagctga ggctgaggac gtgctcttta
     6121 cacctgatgt actgcccatt aatgtagaag aaggaaaggg aaccaccggg tggaccgata
     6181 acggtacaac ggtgactgtt gtcagcagcg tgttggattt ctcctcgtcc gaatatgaag
     6241 acatgggttt gaattccgag accactgtgg atgtgggaga ctttttggta ttctatatag
     6301 gcaactttgg agagattaat gcacaggaca taacctacgg tgaagttacg aaaatcgaag
     6361 ttaatgaaga cagtaccact accattaatt ataatccggt ggaagaaaaa gatattctgt
     6421 cagctatgga gctttacgat gaaacaaagc ttcccaaaga tgagattttg gactccatca
     6481 ataaaaacaa agaagaaata caagaaatca ttgaagccca attgatgggg agcaacttct
     6541 ttgatgaagc cggtgaatat cttgccgagc tggcccttca aaccgatgag gttagggaag
     6601 tatttggcga tgatttgacc ctgtctgact gtactattac ctacgcagac ggcacacctc
     6661 tgggggcaaa tgaactgaca ctgatgggca atatcataga caaggatcag gacggcaaga
     6721 agccgaaggt ctctgtgagt gtttcaaccc taacgactca ctttgatcct aaactcgtcg
     6781 gatccgggat acgtgcggaa gttgcggtaa gctataaatt caagatccag aaaaaaggca
     6841 ataataatat tatggaggtc gagttgactg ctttcttcga gcaggagttt actttcggtt
     6901 ttaacgtcag tggaggtgcc gtttggaaat ggaagtggaa gattatacct tatattgacg
     6961 attatcgaat gaccggaaat ttggatatcg gaacatatac cggactgggt atcaccacta
     7021 ctgcaaaact gactcaagcc aaggaagaat ggggaatgcc atggcccaat agtgcgaagg
     7081 aagccaaagc taccggaaaa atcttcagct tgagtaaatc catcaaggaa ttagtggaat
     7141 cggtgaggac tgtccttccc gaagaagagg ctaccggcag tggcggactg gctgagaagt
     7201 atgcgaggtt tatggaagat gccaatgaag agtgggtgga tctggtttcg gttaacctta
     7261 taaacttacg tagtgcggtg gatccccttc atatccttgc ctttggtctt gacgtcgatt
     7321 ttgtggtttc tgccaacttg aatgtggcta ttggaatgac cttccaatac gagaacttca
     7381 agcgacactc ctttactctg cttctcttca gtaagaagag tgaaagtgaa agcatcgact
     7441 tgtccaccaa cggttaccag tttgacttct atgtcatggg tactgtcggt atcagggcag
     7501 gtgtaagggt aaaggttacg atgggacttt tcagtacaaa gctggctgcc atcgggttgc
     7561 aatttgaagc aggagcctat gtccgattat ggggctactt ctattaccat ctggaaaact
     7621 ggaaggtaga cggaaagtgg caaaaagaaa gcggctactc cggtgcactt cttatcgaag
     7681 ttggtgccta tctggatata aaatttattg ctgaggcttt aaacggcaaa tactcctaca
     7741 cgccttccat ctatgttaag gagtggccgt tgtggtcagc gggacagcgg gaaaatgtct
     7801 atgactttgc ctatgaagat aacctgacct ttgatatttt gaacgtcagc acttataccc
     7861 tccccaaaac agtctttgat atgtattgga tggatatcaa gaccggtgag ctggaggaag
     7921 gttttaataa caagataaag aattttgaca gcaacaaggc acccggcggt gatgatgaag
     7981 agtactttgc cgtggagctt tccaatcgga acttcagcta taatcctgtt aacaatcaga
     8041 ttacagtaaa caccgattcg ggtgaagtgg ctcaaagctg tgaaatgaat attacatgga
     8101 agggtacatc cctgtccgga ctttccgagg tactcagcag aactgtcaag ctcaactgga
     8161 gtaacgagaa aaatgccgga accattgcct ttgaatctaa tggaggctca gttgttccga
     8221 tggtacgttt acttacaggg acacctctca cgggtagaat gccggcacct cctgtaagac
     8281 tgggatatac ctttgccgga tggtatgagg atgctgattt tagtacaata tttgatgctg
     8341 cgaatatgcc tgcaggtaac aagaccctat atgccaaatg gacaccaaat gacgttagtt
     8401 atattgtgga gcattatcaa aaggcattga acggtcagta tgtgctggta aaaaccgata
     8461 aagaccaaaa aggaaaagtc ggtgaacaga ctgctgctgt tgccgagaca tataccggct
     8521 ttacggctca gcctgtaaag cagcaaacca tcgtttccga cggttccacc cgagttaccg
     8581 tttactatga ccgcaatttg tatgatttga aatttgtgta tggcaacggc agtaatgata
     8641 ttagcttaaa agtagcatac ggttctgaca ttgtaaaacc ttccaatcct ttgaaagcgg
     8701 ggtacagctt agacggttgg gatgcggata ttcccagcac catgcctgca aataacctga
     8761 cctttaccgc ccggtggact gaaaggactg atacacctta caaggtgaag cactataaac
     8821 aaaatctgga tgaatcctat aagctggagc agaccgatga aaagaccggc accacaggac
     8881 agctcaccga tgctgtaact aaaacttata caggatttac ggcaaagacc ttcgcacaga
     8941 aaactattct gcccgacgga agtaccgttg tagaaatcta ttatgaccgc gatagcttcg
     9001 ctttatcatg gaatgtaaac ggcggaaaac ctttgacagg atcgtatacc tatggcagcg
     9061 ttaagtacgg aacgcctatc aagaaacccg acatgcctac caaaaccggt catacctttg
     9121 ccggatggta tatggatgag gaactgactg cagcattgga ggataatgct acaatgcctg
     9181 caaaagatat caccatgtac gcaagctggg aggtggaaca gtataccatt agctttgaca
     9241 gcaatggtgg aagcccggta ccacctatta cccaggagta cggtacagcc gtttccgagc
     9301 cggcggctcc ggttagagca ggctataact ttaccggatg gaagctaaac ggagagaatt
     9361 atacattcac taccatgcct ggagaaaata ttacactgat tgcccagtgg acagcccata
     9421 ccaatatacc gtacacggtg gaacactatt ttgagaaagt ggacggaagc tatccttcca
     9481 tccctgatga aaccgagaat ttgacaggta ctaccgattc tacggttact gcaaatgcca
     9541 aggattatac cggatttact tttgactccg gtgtcagcgg aacagttacc tccggcacga
     9601 tttcggcaga tggcagcttg gtattgaaga tgtactacac ccgaaacagt tacactgtga
     9661 catgggatgg caacggcggt aatgtggata ccaatggtgc aacaacgggc agcgtgaagt
     9721 atggtgctga tattaccagt cccgctaatg atcctgtcag aacagggtac acctttaatc
     9781 aatggtccgg atataccgaa aacatgacca tgcctgctga gaatataaca tttaccgcag
     9841 aatggacaat aaatcagtat accattacct ttgacgatat ttctaccgaa gaagacgtac
     9901 aaggtatgtt tataaccagc aggcttatag ttaacggaac aggtactttg actgtcacca
     9961 gcggtacagg aggggcaaaa tataactgcg gtatccgggt tggatcacga ggaaatgact
    10021 ttagaattaa tggccttgag gtaaaggcct ttgcaggtgc tgcaggaagc ggaggcaaga
    10081 gctatggagt attcatggat tcttctattg gaacatatgt tgtccagttg acaaaaggta
    10141 cactggagac tcatggatac gattgtgcgg gatacgctag tctcagtact gccgaatact
    10201 ttattataaa tcaatatcag caaaagtttg ctaccattac tgtcagcgaa aactacgacg
    10261 gaactgatgc aattgatgta gaacagaatg caacctacca aaattacaag tacatgtatg
    10321 tcacaaatcc gtaaagacta aaaccgtggt gggggaaacc ctgcctcggt gcacttaaaa
    10381 gcaaaaactc aaggacatgg agtccctttg ctttttgatc attttctttg tgaaatccgg
    10441 caatcaaaag tttaaggggt atcaaagctc tttgcttttg ggttataaaa agggtgagcg
    10501 tatgaaacta catttatttt tagcggcaat attgatgagc atatttttga ctgtacctgc
    10561 tcaggcagaa gaggctgaac tggtacgtat tgctataatt gacaccggaa tatccactgc
    10621 cgcaattagt gaaaggaatt tagctaaagg acgaaactat attctgtcca atgctactac
    10681 tgaggacact atggggcacg gtacagccgt tgctgccatt attgcaggca gtgaaacggc
    10741 aggggttacc ggcctttatc ccgaggcggt actggtgccc ctggtttatt acagcaaaaa
    10801 cgaggacaat acagctttta agggagattt acccatgctg gcacagatta tccgggacgc
    10861 agtggatgtt tacggttgcc gaattattaa catcagctca ggagcacggg tagatactcc
    10921 aactcttcgg gatgcggtgg catgggcgga acagcgtggt gttttggtgg tttcctgtgc
    10981 cggaaacgac ggtgacggta cggcctatta tccgggagcc ttttccactg tcctttgtgc
    11041 cggaactgta aacccggaag aggatggtcc tgctagcttt tccaaccga
//
LOCUS       DAOOJC010000018        10968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_5419_length_10968_cov_3.634381, whole genome shotgun sequence.
ACCESSION   DAOOJC010000018 DAOOJC010000000
VERSION     DAOOJC010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10968
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5419_length_10968_cov_3.634381"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLA01_00970"
     CDS             <1..234
                     /locus_tag="PLA01_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPC99904.1"
                     /translation="SPELLVEVKENYKEIIEAIDSLKEKQKTAFILRYLRDFSYKEIG
                     KIMGISQEAAKMKVQRAKSALAQRILNMGRKGD"
     gene            236..1921
                     /locus_tag="PLA01_00975"
     CDS             236..1921
                     /locus_tag="PLA01_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPC99905.1"
                     /translation="MKCIYMDEIINCDVDSLCNEKSEEINKHISTCKDCRNYLGALLI
                     SKKYIAKEPKMDKYFYMKVINAVDAGRYKKSKLTFKVLSFFERLKPALKATFGTLAIF
                     VSIALLLTSGIFDNLGSLIGKNNNNIFYSDDPTNLTILQTKGQDIVTEDGEIFNIRGI
                     TLTNNFWGNWVIGESEKLQSEGKDPIIRPLVQDEWVLTEDDFERIKSLGCNTVLYDIN
                     YQLFAEDNPSREANLERLKGHIRRFSSMDIYTAIMLMAPPGLDSANDIHEKNKHGSIR
                     LKSVFEDDIYYEQWIKMWKYLANELKDFKGVAGYGIINQPRTPSDSEGGIEVFTERLN
                     NVCKEIRKIDKKHIIFVPEYNSKEANPGESYWNDETQSNTIDKGEQGIIWERGLVKVD
                     SSNVVYLFHFFEPYSFVNDGIGDFDASSLESQVKSRYEWAKNIGKAPLLTEYGVSRIN
                     SVDKRVEWLQTVHSIFNKYGISASYFQYKTSVGAYVSMETGFNALYGEYTGWKNEISL
                     IPFGFANENVASAAKENHFSEAYEKYYLKNNYIETISILDNQPVLESLQDFWK"
     gene            2091..3326
                     /locus_tag="PLA01_00980"
     CDS             2091..3326
                     /locus_tag="PLA01_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPC99906.1"
                     /translation="MKKRILVALLVFSMLTGLFCFQSLGESDRDLKYGAWIGSWPSKA
                     AIDSFQELQGKKMDIIHQFIDWSTTFSYIKSYADAVYDNGSILMITWEPWEYNTAAIK
                     NGNADAYITQMAQDMKAYGKEIWLRPLHEANGNWYPWAIGYPGNVNTNDTYIAAFRRI
                     VDIFRQNGATNVKWIYTVNCDNVGDNTSYLGYYPGDNYVDFTSIDGYNWGTTQDWGSK
                     WQTFDEIFSRAYNALSSIDKPMFIAEFASAEQGGDKSRWITDTYNSIKNSYTKIFAAV
                     WFHENKETDWRINSSSSALEAYKNAIGAYSSTSTPTKTPTSTPTKPPTPTPTKSPTPT
                     PTSTASIKLGDVNFDDEVNSTDLTILKRFILRTYTPDYSKLEMFKKAADFNEDNSVDS
                     SDVTLLKRYILRKYPYNNR"
     gene            complement(3506..3991)
                     /locus_tag="PLA01_00985"
     CDS             complement(3506..3991)
                     /locus_tag="PLA01_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPC99907.1"
                     /translation="MIIVYESKTGFTKKYADMLAAKTGLKVFPVKEISKVGKDEEIIF
                     LGWMKAGKIQGLNKLREYNVKAVCGSGTARNAEPDAQTVIARNKIGNKPFFYLRGGCL
                     PLREIKGMDKILLSMFVKMLKSRKDKDEKLEEAISNIENGFDGVKEENLKPVLEWLTE
                     K"
     gene            complement(4153..5250)
                     /locus_tag="PLA01_00990"
     CDS             complement(4153..5250)
                     /locus_tag="PLA01_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99908.1"
                     /translation="MKITVLIQMIFGVGISYLISNIVYEFTKKENLSIIASVFYAAFI
                     PFLYFELAILSETTAAFFILLSFTCYIKLLKNKDRYLMRVLLISIFTLIAAFTRPVYL
                     VMAPLYVVYFMFDFRANKLKLGQSIKYLAVFALPILIGVIGWSYVNLKYNGTFSFATG
                     RGFAAMEMAGDYIEYASDDHPYNIIKEVYIRERDINIRNGAPHIDTIWGITDELQEKT
                     GLSYNELSKEVKKMCTQVMLKKPDIYFKSVIRSMINFWKTFGRPIFTESNISTPIKYI
                     NIVQRCILILLQLPFLFSPLAYLIRRKKLKLNGDVRLICSFVYILIIGISILISIIEC
                     SEGRFAMPTFPLLIITTFILYYNILGKPKVG"
     gene            complement(5678..5989)
                     /locus_tag="PLA01_00995"
     CDS             complement(5678..5989)
                     /locus_tag="PLA01_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99909.1"
                     /translation="MTLLTTVFAAIICTIIWYKNAPNSEMKVGILCWMYWGASLMWLV
                     DAVFEYVELKAEYFTPAPVDMLNDFYLGLSVVALGLIIWLVILLIKDPKGVVKAALFK
                     K"
     gene            complement(5986..6405)
                     /locus_tag="PLA01_01000"
     CDS             complement(5986..6405)
                     /locus_tag="PLA01_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99910.1"
                     /translation="MACFIVPVAEAVVTTVITKAVKSKDTKPEALKADTVSFSAKHKI
                     PFSRKLKWLSNMLWGGSVLLAFEHVWHGEVVPWFPFLTAASDPAETSEMLFEMATVGV
                     SMALIVTAVWGVMLLVTNAMEKKAIKAHPAQIKEGCI"
     gene            complement(6736..8070)
                     /gene="mgtE"
                     /locus_tag="PLA01_01005"
     CDS             complement(6736..8070)
                     /gene="mgtE"
                     /locus_tag="PLA01_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPC99911.1"
                     /translation="MNEKILALIEQGKYADARKEIVEMNVVDIAQLFEELDRQKLLVV
                     FRILPKDVASGVFSYIPNELQKYIIESITDKEIKTIIDELFLDDTIAFLEEMPSNIVK
                     KVLKNTDEETRKLINQFLNYPEDSAGSIMTIEFVDLKKEMTVKQALEHIKDTGVDKET
                     INNCYVIDNNRILEGVISIRKLILSDGSAVVKDIMEKDVVYINTHDDQEKMASLFKKY
                     DFLSMPVVDNECRLVGIVTIDDAVDIIEQENTEDIHKMAAMKPSEDKYLKTNALVLAK
                     NRITWLLILMISATFTGIIIRKFEDVLQSVVVLASFIPMLMDTGGNAGSQSSALVIRG
                     LALGEISLKDALKVLWKEIQVGCLVGLALSIVNFVRLYFVEKINLSISITVCITLLFT
                     VILAKIAGGILPIIAKKCKIDPAIMAGPLITTVVDAVTLTIYFSTAVWLLGI"
     gene            complement(8756..9490)
                     /locus_tag="PLA01_01010"
     CDS             complement(8756..9490)
                     /locus_tag="PLA01_01010"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPC99912.1"
                     /translation="MKKLKKGFEVYKMSRYKCILFDCMETVIDVIEKPDLRLYAYWAY
                     SGCGYESLWESFDAFAQSYSNIRNAFRNSYKEFEEYNLFDIFEYMARQLSDDGNVKEK
                     VTKALFNNYWGNYKSNCIVYDDVKSVLSELSDKYKLGIVSNFIVKDGVEELLEINGIH
                     KYFDFVVTSIKVGWRKPHHRIYDTALDLAKVSKEEILFIGDDYACDYEGPTKYGFDAV
                     LLDKECIYRNVEKRIVTLDKIVDYLD"
     gene            complement(9487..9582)
                     /locus_tag="PLA01_01015"
                     /pseudo
     CDS             complement(9487..9582)
                     /locus_tag="PLA01_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716231.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
     gene            complement(9542..10525)
                     /locus_tag="PLA01_01020"
     CDS             complement(9542..10525)
                     /locus_tag="PLA01_01020"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HPC99913.1"
                     /translation="MFDVIAIGELLIDFLPSGMNENGLPLFSANPGGAPANVLAMLAK
                     LGAKTAFVGKVGKDGFGQFLKATLLNAGIDTSNLLMSTTEMTTLAFVHLDDKGDRSFS
                     FYRKHCADVSLDAGELDKDLLSSCRFLHFGAVSLTDEPSRTATLEAARIAKESGAIIS
                     YDPNYRSALWDNEQQAVKYMLKAMEYADLVKVSEEELSLMTGCTDYSLGAEKLLAIGL
                     SVIFVTCGEKGSYVFTESSCANHGGYRVKAVDTTGAGDTFMGTMLWQLKDKSLDEITQ
                     ISEEEWEELLAFSNAAGALVATKKGAIPAMPDLDTIYAFRRSSHAENPPVG"
     gene            complement(10576..10917)
                     /locus_tag="PLA01_01025"
                     /pseudo
     CDS             complement(10576..10917)
                     /locus_tag="PLA01_01025"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF234 domain-containing protein"
BASE COUNT         3513 a         2281 c         1826 g         3348 t
ORIGIN      
        1 agtcctgaac tgttagttga ggtcaaggag aattataaag aaataattga agccatagac
       61 agccttaagg aaaaacaaaa gacagctttt atattaaggt atttaagaga cttttcctat
      121 aaggaaatcg gaaaaataat gggaatttca caggaagctg ccaaaatgaa agttcagaga
      181 gcaaaatctg cacttgccca gaggatacta aacatgggaa ggaaaggtga ttaaaatgaa
      241 atgtatatat atggatgaaa taataaattg cgatgtggat tctttgtgta atgaaaaatc
      301 tgaagaaata aacaagcata taagcacttg caaggactgc agaaattatt tgggggcact
      361 tttgatatcc aaaaaatata ttgccaaaga accaaaaatg gataagtatt tttatatgaa
      421 ggttattaat gctgttgatg ctggcaggta taaaaaatct aaactgactt tcaaggttct
      481 gtcgtttttc gaaagactaa aacctgcttt gaaagcaact ttcggaacct tggcaatctt
      541 tgtgtcaata gccctgttat taacgagcgg aatatttgat aatctcggta gcttgatcgg
      601 taaaaacaac aataatattt tctatagtga tgacccgaca aatttaacta ttttacaaac
      661 aaagggacaa gatattgtta ctgaggatgg tgaaatcttc aatatcagag gtattaccct
      721 gaccaacaac ttctggggca actgggtcat cggagaatcg gagaaattgc aaagtgaagg
      781 taaggaccca attataaggc cgcttgtgca agatgaatgg gttttgaccg aggatgattt
      841 tgagcgaatt aaaagcctcg gatgcaacac ggttttatac gacatcaatt atcagctttt
      901 tgcagaagac aacccaagca gggaagctaa tcttgaaagg ctgaaaggac atatccggcg
      961 tttttcttcg atggatattt atactgccat aatgcttatg gctcctcccg ggttggattc
     1021 ggccaatgat attcacgaaa agaacaaaca tggttcaatc cgtttaaaat ccgtatttga
     1081 agatgatatt tactatgagc aatggataaa aatgtggaag tatttagcca atgaactcaa
     1141 agactttaaa ggtgtggcag gatacggaat aataaaccag ccaagaaccc ccagtgacag
     1201 tgaaggtgga atcgaggtat tcacagagcg tttaaacaat gtatgtaagg aaatacgtaa
     1261 aatcgacaaa aaacatatca tatttgttcc tgaatataac agcaaggaag ccaatcccgg
     1321 cgagtcatat tggaacgacg agacccaaag taatacaata gacaaaggtg aacaaggtat
     1381 tatttgggaa agaggtttgg taaaggtaga ttcatcaaac gtggtatacc tgttccactt
     1441 ttttgaaccc tacagttttg tcaacgacgg tattggggac tttgacgcct caagcctgga
     1501 gtcccaggta aaaagccgtt atgagtgggc taaaaacatc ggcaaagccc cgttattaac
     1561 cgagtatgga gtttcccgga taaacagcgt ggataaacgc gtggagtggc ttcagaccgt
     1621 ccacagtatt ttcaataagt acggtatatc tgcatcctat tttcagtata agacttctgt
     1681 aggtgcttat gtaagtatgg aaacgggttt taatgcttta tacggtgaat ataccggctg
     1741 gaagaatgaa attagtttga ttccttttgg atttgcaaat gaaaacgttg cttctgctgc
     1801 aaaagagaat catttttctg aagcatatga aaagtattac ttaaaaaaca attatataga
     1861 gactatttca atactggaca atcaaccggt ccttgaatca ctacaggatt tttggaaata
     1921 aagactatat aaaacgttac ctttatattt ttcgggagta ttaattactg ggggtattga
     1981 tgtttttact ccttgtatat aaagttataa atccaagtca tgaaaggagg atattttaga
     2041 gaaactgata ttttttaata aattttaatt ttttgaatgg gaggaatagt atgaaaaaaa
     2101 gaattttagt ggctttactt gtatttagta tgctcaccgg tttgttttgt tttcaatccc
     2161 ttggtgagtc agacagagat ttgaaatatg gtgcatggat aggaagctgg ccttcaaaag
     2221 cagctattga cagttttcag gaacttcagg gcaaaaagat ggatattata caccagttta
     2281 ttgactggtc caccactttt tcctatataa aatcctatgc tgatgctgtt tacgataacg
     2341 gttcaatatt aatgattacc tgggagccgt gggaatataa cactgcggcc attaaaaacg
     2401 gtaatgcaga tgcttacata acccaaatgg cacaagatat gaaagcctac ggcaaggaaa
     2461 tttggctaag acctctccat gaggccaacg gaaactggta tccatgggct ataggatacc
     2521 caggcaacgt aaacacaaac gatacctaca tagctgcctt caggcgtatt gttgacattt
     2581 tccgtcaaaa cggtgccacc aatgtcaaat ggatatatac tgtaaattgt gacaatgtag
     2641 gtgacaacac aagctatctg ggttattatc ccggggacaa ctatgtagac ttcacttcca
     2701 ttgacggata taactggggt actactcaag actggggaag caaatggcaa accttcgacg
     2761 agattttttc acgagcttat aatgccttat catcaataga caaacccatg tttatagcag
     2821 aattcgcatc agccgaacaa ggcggagaca aatcaagatg gattacagat acatataact
     2881 ccataaaaaa ctcttacact aaaatatttg ctgccgtttg gttccatgaa aacaaagaaa
     2941 ccgactggag aatcaattcg agttcttcag cccttgaagc atataaaaat gcaataggtg
     3001 cctattcatc aacttcgact cctaccaaaa ccccaacttc aactcctacg aagccaccga
     3061 ctcctactcc tacaaaatcc ccgactccta ctcctacatc tactgcttca ataaaactcg
     3121 gggatgtaaa ctttgatgac gaagtgaatt ccacagactt gacaatattg aaacgattca
     3181 tcctcagaac gtatactcct gattacagca aattagaaat gtttaaaaaa gcagcagatt
     3241 tcaacgaaga taatagcgtt gactccagtg acgtaacatt attaaaaagg tacatcttaa
     3301 ggaaataccc gtataataac agatgagtcc gcaattttta tgaatcgtga ccggatggat
     3361 taaatgaata tgagcaaaaa tgatgggggc atcgagcccc catcgctttt tggtcatttc
     3421 acttcgtgaa atccaactaa aaaaaggagg ataccggtat cctccttttt ttagttaaaa
     3481 atttttcacc cgtcatgccg cttttctact tttctgtgag ccattcaagc acaggtttga
     3541 ggttttcctc ttttacaccg tcaaaaccgt tttcgatatt tgaaattgct tcttcgagtt
     3601 tttcatcttt atctttgcgg cttttaagca tcttcacaaa catggaaagc aatattttat
     3661 ccatgccctt tatctctctc aaaggcaaac atcccccgcg aagataaaaa aacggtttgt
     3721 tcccgatctt attccttgcg ataactgttt gggcatcggg ctcggcattt cgtgcagtac
     3781 ccgatccgca aacagcttta acgttatatt ctcgcaattt attaaggcct tgaatctttc
     3841 cggctttcat ccagccaaga aaaatgattt cttcgtcctt accaaccttt gaaatttcct
     3901 ttaccggaaa aacctttagt cctgtctttg ctgcaagcat gtcagcatat ttttttgtaa
     3961 aacccgtttt tgattcgtaa acaattatca tgcttccatc tccttctaat aaatttcggc
     4021 tcactaatat gccataccgg cactgacaag ccattatata ctatccttca aattctttag
     4081 ccttgatccg tttttatgtc cattaatatt ccttcagttt tcaaatatcc aactcgccat
     4141 cacatctata actcatccaa cttttggttt ccccaatata ttatagtaca aaataaaagt
     4201 agttataata agtaatggaa aagtaggcat ggcaaatcta ccttccgagc actcaataat
     4261 agatattaat attgagatac cgattatcaa gatatacaca aaagagcaaa tcaatctaac
     4321 atccccattg agtttcaatt tcttccttct tataagatat gccaaaggcg aaaacaaaaa
     4381 aggtaattgt aataaaatta atatacatct ttgaacaata ttaatatact ttataggagt
     4441 gcttatattc gactcagtaa aaatcggtct cccaaatgtt ttccagaaat ttatcattga
     4501 tctgataacg gacttgaaat atatatcagg cttttttaac atgacttgag tacacatttt
     4561 cttaacctct ttggaaagtt cattataact aaggcctgtt ttctcctgga gttcatcggt
     4621 tatcccccat atagtatcaa tatggggagc accatttcga atattaatat ccctttccct
     4681 aatataaacc tctttgataa tattatatgg atggtcatca gaagcatact caatatagtc
     4741 tcctgccatt tccatagcag caaatccacg acctgttgca aatgaaaagg taccgttata
     4801 tttaaggtta acatatgacc acccaataac gccaataagt ataggcaaag caaaaactgc
     4861 caggtatttt attgattgtc caagcttcag cttattggct ctaaaatcaa acataaagta
     4921 aacaacatat aatggagcca tcactaaata aaccggtctt gtaaacgctg caatcaatgt
     4981 aaaaatacta attagtaaaa ccctcatcaa atacctgtct ttgttcttga gaagcttaat
     5041 ataacatgta aaagataata gtataaaaaa ggctgcagta gtttcactta aaatagcaag
     5101 ttcaaaatac agaaagggaa tgaaggcagc gtaaaaaaca gaagcaatta ttgaaagatt
     5161 ctctttcttt gtaaactcat agacgatatt tgaaattaaa taagatatac ctactccgaa
     5221 aatcatctgt atcaaaactg taattttcaa attaaatccc gcaagcaata aaatcaatga
     5281 ataacccggt gttctaagtc cgttaaagcc ggaaaagtca aaattcacaa tatttttagc
     5341 aagatcaata tatgattgcg tatccgggca aattatcgga tttgcaataa agaaaactgc
     5401 gagccgaaca attgttatgc atacaatata tatcattagc tttgtcttaa atttcactag
     5461 ctataactcc cttttatgta tcgatgtttt tattgattat tttaattata acaatacaac
     5521 aatggatatt attaatcaat aacaataatt tattctgaaa aaaaagctcc gggggcatca
     5581 aacacccatc gctttttggt catttcactt cgtgaaaccc cacttgccag aacagccccg
     5641 cattttgaaa gcagggctgt tctttattta gctcttacta ttttttaaac aacgctgctt
     5701 tgacaacacc ctttgggtct ttgatgagaa gaatcacaag ccagataata agccccagtg
     5761 caacaacaga tagtcccaaa tagaaatcat tcagcatatc aaccggtgca ggagtgaaat
     5821 actctgcctt aagttcgacg tactcgaaga ccgcgtccac cagccacatc agcgaagcac
     5881 cccaatacat ccagcagaga ataccgacct tcatttcact gttgggagcg tttttatacc
     5941 aaatgattgt gcaaattatc gctgcgaaaa cggtggtaag taaagtcata tacatccctc
     6001 cttaatttga gcaggatgtg cttttatagc ctttttctcc atagcattgg tcacaagcag
     6061 catgacaccc cagacagcgg taacaattaa ggccatggac acgccgactg tagccatttc
     6121 aaagagcatc tccgatgtct cggccgggtc ggaggccgct gtcaagaacg ggaaccatgg
     6181 cactacctca ccgtgccata catgctcaaa ggcaagcaga actgatcctc cccatagcat
     6241 attgctgagc catttcagtt ttcttgagaa tgggattttg tgttttgccg aaaagctgac
     6301 ggtgtcagcc tttaaagctt ccggtttagt atctttagat tttacagctt tggtaataac
     6361 ggtcgttaca acggcctctg caacaggtac aataaaacat gccataataa aacctcctcg
     6421 tcataatgag ttatttggta taacaatcaa aaggaatatg ccattaatcc tcgccatgaa
     6481 cgccaacaaa aactccggtg gtatcgagcc ctccatcgct ttttggtcat ttcacttcgt
     6541 gaaatcccac taaaaaaggc atacggatct tcttttgaaa aaccatatgc cttcaatagc
     6601 atacattaat gcagattact gcatatagtg ctagaaccgt attgcttctg tattcttgaa
     6661 ccagattcgt ataaatatct agaatattat accataacat gcaaagttaa tcaagatggc
     6721 acatgtaaaa atcatttata ttcccaataa ccataccgct gttgaaaaat atatagtcag
     6781 agttacagcg tcaacaacag ttgttatcag cggacctgcc ataattgcag ggtctatctt
     6841 acattttttt gctataatag gcagtatccc gcccgcaatt tttgctaata taactgtaaa
     6901 cagcaaggtt atacacacag ttatagatat tgataaatta attttctcca caaaatataa
     6961 ccttacaaaa tttactattg agagagccag cccgacaaga cagcctacct gaatctcctt
     7021 ccaaaggact ttcagagcat cctttaggct tatttccccc aaggccaagc ctctgataac
     7081 aagtgccgat gactgagacc cggcatttcc acctgtgtcc atgagcattg gaatgaatga
     7141 agcaagaacc acaaccgatt gcaacacatc ttcgaatttt cttataataa ttcctgtaaa
     7201 agtcgccgaa atcattagga ttaaaagcca ggttattcta ttctttgcta acaccaatgc
     7261 gttcgttttc aggtatttat cttcagacgg cttcatagca gccatcttat gaatatcttc
     7321 ggtattttcc tgctctatga tgtctacagc gtcatctatt gtcactattc caaccaacct
     7381 gcattcgtta tcaaccacag gcattgatag gaagtcatat ttcttgaata acgatgccat
     7441 tttttcctga tcatcatggg tgttaatata cacaacatct ttttccataa tgtctttaac
     7501 tactgctgaa ccatcactta atatcagttt tctgattgat attacgcctt ccagtattct
     7561 gttgttgtca atgacatagc aattgttaat tgtctccttg tcaacccctg tatccttgat
     7621 atgctctaat gcctgtttta ctgtcatttc tttttttaag tcaacaaact caatagtcat
     7681 tatgcttccg gcagaatctt caggatagtt caaaaattga tttataagtt ttctcgtttc
     7741 ttcatcggta ttcttaagaa ccttttttac tatgtttgaa ggcatttctt ccaaaaaggc
     7801 aattgtatcg tccaaaaaca gttcatctat aatagtcttt atttccttgt cagtaataga
     7861 ttcaatgata tatttttgaa gttcattggg tatataagag aaaacaccgg atgcaacatc
     7921 tttaggcagt atcctgaata ccaccaaaag tttctgccgg tccaattctt caaaaagttg
     7981 tgcaatgtca acaacgttca tctcaacaat ttcttttctt gcatcagcat atttgccctg
     8041 ttcaataaga gcaagaattt tttcattcat tttgcagccc tccttcaata gggccacact
     8101 atcccattag tagctatggc cctgaataaa ttattcactt tttcattaca ataaccgggt
     8161 aaaggttcgg gatccataac taccacctct gctttcttga aataatataa aaaattacct
     8221 ttaacctaat ataaagcaat agctataaca ggcaataaaa gctataagca atttaatgcc
     8281 aatataaccc taaataatga ttattaaatt aatcattaaa tttaacaatt aattatgcaa
     8341 actgattttt cgaagaaaac gttaaaaaat gacaatagag attcgtttct gggaaaaaaa
     8401 catatacgaa aaaatagaat ttgaattata acattaagca aactctcgtc agcgaagaac
     8461 agagtttgac agttgtaatt ctatttttcc cgtagtatat acgtttatct ccacagtcaa
     8521 tattactact gccagattcc actcttctca cctccgaaaa ttcaaataaa cacaagtccc
     8581 ctaataataa agtatttgct tttattacgg taacagccgt aaacttccag tgtattcatc
     8641 tatcgagttt ttatccctat tttattgtat gcttatgccc tataattgtc aactggtcta
     8701 atattttttt ctctttgata tatagggtat tatgcttcat ataatagtaa gatattcaat
     8761 ctaaataatc gactatttta tcaagggtta ctattctttt ctctacattg cggtaaatgc
     8821 actccttatc tagcaatacc gcatcaaacc catattttgt cggtccttca tagtcacaag
     8881 catagtcgtc tcctataaaa agtatttctt ctttcgatac tttcgccaag tccaatgctg
     8941 tatcgtatat tctatgatgg ggctttctcc atcctacctt tatagaagta acgacaaaat
     9001 caaaatattt atgaatgccg ttaatttcaa gaagttcctc tactccatct ttcactataa
     9061 agtttgatac aattcctagt ttatatttat cggataactc ggataaaaca gacttaacgt
     9121 catcataaac aatacagttc gatttgtagt ttccccaata attattgaaa agtgcttttg
     9181 ttaccttttc tttgacgtta ccgtcatccg aaagctgtct tgccatatat tcgaatatat
     9241 caaacagatt atattcttca aattctttat aactatttct aaatgcattt cttatattac
     9301 tatagctttg tgcaaaggca tcaaagcttt cccataaaga ttcataaccg cagccggaat
     9361 aagcccaata cgcatagagt ctcaaatcag gcttttcaat aacatcaatt acagtttcca
     9421 tgcagtcaaa taatatacat ttatatcttg acattttata tacctcaaaa cctttcttta
     9481 atttcttcaa ctgctcaata tcttcaccat tggttcgcct tcagccaatt cgtcaaccgg
     9541 cttatccaac cggcggattt tctgcatgag aggatcttct aaaagcatat atcgtgtcca
     9601 aatcaggcat tgcaggtatt gctccttttt tcgtagccac aagggcacct gctgcattgg
     9661 aaaaggccaa aagttcctcc cattcctcct cggatatttg tgttatctcg tccaacgact
     9721 tatcctttag ctgccacagc atagttccca taaaagtgtc cccggctcct gtggtgtcaa
     9781 ctgcttttac tctatatccg ccatggttgg cacagctgct ttcggtaaaa acataagatc
     9841 ctttttcacc gcaggtaaca aagatgacag acagaccgat tgcaaggagt ttttcagctc
     9901 ccagggaata atccgtacat ccggtcatta gcgaaagttc ttcttcgctc acttttacca
     9961 aatcagcata ttccattgcc tttagcatat attttacagc ttgctgctca ttatcccata
    10021 atgcagagcg gtaattcggg tcataggaaa ttattgctcc cgactctttt gcgattcttg
    10081 ctgcctccaa agtagcagtc ctgctcggct catccgtcaa cgagacggca ccgaagtgca
    10141 gaaaacggca ggaactcaac agatccttgt caagctcacc ggcatccaag ctgacatcgg
    10201 cacaatgctt tcgatagaaa ctaaaggatc tgtcgccctt gtcatccaga tgaacaaagg
    10261 ccaaagttgt catttcagtt gtgctcatca aaaggttgga cgtatcaatc ccggcattta
    10321 aaagagttgc cttcaaaaac tgtccgaaac cgtctttacc gactttgccg acaaaagccg
    10381 tttttgcgcc cagtttcgca agcattgcaa gaacgtttgc cggggcaccg ccgggatttg
    10441 ctgaaaaaag gggtaatccg ttttcattca tacccgaagg caaaaaatct attaacaatt
    10501 cacctattgc aattacatca aacatatatt cctcaccaac ttattaatca agaatgctta
    10561 tgtatttctc ttattttatt cacacaacac aacatcttcc cttttctttg ctaattcaac
    10621 cagttcatct gtgaatccgt taattccaaa taatataaag tgttcccgac gtttttccct
    10681 tttccactct acaaacttcg ccttttcttc aagagcagca agtacattca ctccaacctt
    10741 gccttcccaa tatttacact ccccaaatat tatatcattt ccggtgctgt caaacgccac
    10801 aatgtcaatt tctttatcgc cattccacca tcttcccacc ttgtcaaaag tgaaatccca
    10861 ctttccttgg gcattcagcc tccacatctc ctctatacaa atatcttcat atacatagct
    10921 tacatgtcca tctgtgaact accaccgcct atagaggcag atggcttc
//
LOCUS       DAOOJC010000019        10735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_5547_length_10735_cov_3.746348, whole genome shotgun sequence.
ACCESSION   DAOOJC010000019 DAOOJC010000000
VERSION     DAOOJC010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 10735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10735
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5547_length_10735_cov_3.746348"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLA01_01030"
     CDS             <1..434
                     /locus_tag="PLA01_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99914.1"
                     /translation="YSKRQAFYLELMDISLDLAADEIHNDVIKSELTEEEAGCVDVTM
                     MRSGNPKPKFYDALAKEKWFTVNTVIPENYLFNELHQFYISINLYDYRFGKLTEEQQK
                     NVLGSFERIEKRILDKYGDKVSFEMRFDDQHKVKYNNGVKE"
     gene            567..2294
                     /locus_tag="PLA01_01035"
     CDS             567..2294
                     /locus_tag="PLA01_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC99915.1"
                     /translation="MKNFIRILKQGRKYWKYLIVAGISLLAITSLNLITPWKVRELVD
                     ILSRDNSDKMTSIRNIAVILVVAYVARAVFTYLNRFYSHVAAWNLVEDMRVVVYEKLQ
                     KLSLSFYHDKQTGQLMSRAINDTATFEVLIAHAVPDLITNLLILIGTGILLFVISPLL
                     AVLTLIPIPFLILGGAIFTKKILPNFREAQGKLGELNAVMQDNISGMKEIQAFNQQSR
                     ERKRIKKGAKKYTSALLHALKLSAVFHPAVEMISSLGTVIVVAFGGWLALRNYVDTAD
                     IVGFIMFLGLFYQPITTLARVIEDLQQATAGAERVFEIIDTEPDIVDSEGAVEIERAK
                     GEISFKNVSFHYISQSPVLKNISFTAKPGQMIALVGPTGVGKTTVISLMARFYDPTSG
                     EIRIDDRNIKDVTVASLRNQISIVLQDVFLFNGSVADNIAYGSREASFEDIVNAAKIA
                     RAHEFITEMSEGYDTVIGERGVKLSGGQKQRLSIARAVLRDTPILILDEATASVDVET
                     ETQIQQAIGELAGSRTIIVIAHRLSTVKQADNILVLKEGEIVESGTHEELIKLNGLYR
                     HLCEVQFGG"
     gene            2435..3643
                     /locus_tag="PLA01_01040"
     CDS             2435..3643
                     /locus_tag="PLA01_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPC99916.1"
                     /translation="MYCVKELTKDLYWVGGSDRRLALFENVFPVPRGVSYNSYLLMDE
                     KTVLLDTVDKAVSGQFFENLEFVLGERKLDYLVVNHMEPDHCGTMCELVLRYPDVKIV
                     GNDKTVAMIKQFFKFDIDSRAVIVKEMETLCTGKHTLTFIMAPMVHWPETMVTYDVKE
                     KVLFSGDAFGTFGAINGNIFADEVDFEHEWLKDARRYYTNIVGKYGAQVQTLLKKAQN
                     IEINMICPLHGPVWRTNIDWFVEKYQKWSTYTPEENSIMIAYASVYGNTENAVNILAS
                     KLADAGVKRIAMYDVSVSHPSEIVAEAFRCSHLVFASTTYNTGVFCNMEKVLLDLKAH
                     NLQNRTVAVIENGTWAATSGKLMKGMISEMKNMTILDSTVSIKSSLKENQIEEIDAIA
                     AAILNSLKTA"
     gene            3963..4475
                     /locus_tag="PLA01_01045"
     CDS             3963..4475
                     /locus_tag="PLA01_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99917.1"
                     /translation="MSKRVIGIVFICIVLFVIAGCGAKENTPVPKTDEISGYSWKATD
                     ASVLYLESDGTFKWYLHEKIRSDSYYVGTYSVYNGQEAVDYMSTELKEYNVTSQMQMD
                     MIESVEDASLENYYCLVLNNERCIVDGKNTLRDKTVVPYYGFYYAEDEFLDLVNLVTE
                     NYHQFTKYTE"
     gene            complement(4556..5146)
                     /locus_tag="PLA01_01050"
     CDS             complement(4556..5146)
                     /locus_tag="PLA01_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPC99918.1"
                     /translation="MNLKGTKTAENLLKAFAGESQARNRYTFYASVADKEGYKQIKNI
                     FIETADNEKEHGKRFYKFLLEGFQGELPAMIEINASYPVAQGSTLDNLKAAASGENEE
                     WSDLYPAFAKVADEEGFPEIAAAFRMIAKAEVNHEVRFNKLAANIENGTVFKKGDKVY
                     WKCGNCGYIHEGPEAPDKCPACIHPQSYFEVFVETY"
     gene            complement(5166..5588)
                     /locus_tag="PLA01_01055"
     CDS             complement(5166..5588)
                     /locus_tag="PLA01_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPC99919.1"
                     /translation="MNLSTEEVKVYLLKHNIKPSYHRVAILKHLLSEKNHPSVDKIYN
                     ELVKEMPTLSKTTVYNTLNLFSGVSIVRVITIEENETRYDGDVSDHGHFKCEKCKTIY
                     DFQIKSVDFETDDLSSFKINEKSVYYKGICPKCLDNKK"
     gene            5862..6914
                     /locus_tag="PLA01_01060"
     CDS             5862..6914
                     /locus_tag="PLA01_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPC99920.1"
                     /translation="MGVTLTDFISQLLSNPALIITTLLTLGVIFVNGWTDAPNAIATC
                     VSTRSIGPRSAIAMAALFDFLGVFFMTMFNKTVAETIYKMVDFGGDPKEALVALCAAL
                     IAIVLWAVTAWWFGIPTSESHALIAGISGAAIALQRGIGGINFDQWINVVYGLALSLV
                     LGFIMGWSAVKTVELIFRRVNRTKTFSLFKNAQILGSAGMAFMHGAQDGQKFMGVFLL
                     GMFLAQGQANVTEFTIPTWLIILCSTVMALGTSIGGYRIIKAVGMDMVKLEKYQGFSA
                     DLAGGICLFIASISGLPVSTTHTKTTAIMGVGAAKRISSVNWGVVKEMVTAWILTFPG
                     CGLLGFLMALLFMRIF"
     gene            6955..7578
                     /locus_tag="PLA01_01065"
     CDS             6955..7578
                     /locus_tag="PLA01_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPC99921.1"
                     /translation="MARKKNEYYYDTFVELVDYSCQAANLLNDIMNNFDAAKLHEKME
                     EMHAIEHAGDKARHVMIKKLVKEFITPIEREDIMALADAIDNVTDSIEDVVMRMYMFN
                     VVNIRERALEMTKVVVKCCNSLKQALAEFHNFRKSKTLHQLIIEVNQLEEEGDRLFTE
                     ATRDLYVNCNDFKEVAAWDIAFYYLEKCCDACEDVADAIENVIMKNS"
     gene            7880..8626
                     /locus_tag="PLA01_01070"
     CDS             7880..8626
                     /locus_tag="PLA01_01070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPC99922.1"
                     /translation="MSNGIDISVVVPAHNEGKYVKRCIDSIRESADEFEGNVEIIIVC
                     NRCTDRTEDIAKENGAITVKNEDRCIAKVRNAGIDAARGKIIITIDCDNRMTKGTISE
                     VYHLLGSGKYIGGGAPIRFERYSFPLWCNDILCRFSFAVTGLYCGIIWATKDAFDAIG
                     GFVEKRAMEDVSTAKMLKKYGKSKGMKYTTLKKNYLINSTRKYDDLGDWLYFKLMVKN
                     AGTLIKAAMGNTQEYDKLIDEMFYDYNDKH"
     gene            8775..10001
                     /locus_tag="PLA01_01075"
     CDS             8775..10001
                     /locus_tag="PLA01_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPC99923.1"
                     /translation="MKLNKKLICGALTVGLVAGSSVFAFAESISYKVQPGDAFWKISS
                     KYGISTVELMKANNATENTVLYPGQIITVPLNNQVVHTVKSGDTYWIISQKYGVDLQK
                     LLSANNANQNSILYVGDKVVIPSANQTSGYKTYTVQKGDTYWTVSQKFNVNFTELLKI
                     NGANDKSYLDIGQIIKIPSASSSTTSPTQPGETYKDYTVQKGDTLWSISEKYGITVND
                     LAKANNISTSAVLNIGQKLKVPVINVPVKSTPGAQYGELLDWWTEAQYVIPRGCTFEV
                     VDFYTGKSFMAKRTAGSNHADCETLTVKDTNIMKEIWGGFSWVRRPVIIKYNGRKIAA
                     SMTAMPHAGNDSAPGGAWVSWRSGDYGAGTNHDYIKGNGIDGHFDIHFYNSTRHKDGK
                     VDTNHQNCIQIAAGKK"
     gene            complement(10167..10532)
                     /locus_tag="PLA01_01080"
     CDS             complement(10167..10532)
                     /locus_tag="PLA01_01080"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPC99924.1"
                     /translation="MDQVICIPTSPSPARITISIAAKTLSLYREGRLVKSYPVATGKP
                     TTPTPRGTFTIVNKQINPGGPFGTRWMGLSIPHYGIHGTNNPASIGTAASNGCVRMFN
                     EDVNELFNQVSVGTIVNIY"
     gene            complement(10569..10670)
                     /locus_tag="PLA01_01085"
                     /pseudo
     CDS             complement(10569..10670)
                     /locus_tag="PLA01_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
BASE COUNT         3346 a         1757 c         2419 g         3213 t
ORIGIN      
        1 gatatagtaa gagacaggcg ttttatctgg agttgatgga tatatctctt gatttggctg
       61 ctgatgagat acataatgat gttattaaaa gtgaacttac agaagaagaa gcaggttgtg
      121 ttgatgttac aatgatgcga agcggcaatc caaaacctaa gttttatgat gccttggcta
      181 aagaaaagtg gtttacagta aatacagtaa tacctgagaa ttatttgttt aatgaattgc
      241 atcagtttta tattagtata aacctttatg attacagatt cggtaaatta acggaggagc
      301 aacagaagaa tgtacttggc agctttgaaa gaattgagaa aaggatactt gataagtacg
      361 gagataaagt gtcatttgag atgcgttttg atgaccaaca caaagtaaag tataataatg
      421 gggttaaaga atagtatggc tgcggttttc tgatttcttt tacaggacct atatggagca
      481 cagactataa gatgaccttg aagcttctta acgtcagaga ttttgttact atggagcatt
      541 cgtgttttat ggggagggag tacacaatga agaattttat acggatactg aaacagggga
      601 ggaagtattg gaaatacttg attgtggccg gaataagcct gcttgccata acgtctctta
      661 atcttataac cccgtggaaa gtcagagaat tggtggatat actgtctaga gataacagcg
      721 ataaaatgac atcaatacgc aatattgcgg ttatacttgt ggttgcctat gttgcaaggg
      781 cggtttttac atatttgaac cggttttaca gccatgtggc tgcatggaat ctggtggagg
      841 acatgagggt ggttgtgtat gaaaaactcc aaaagctgtc cttgagcttt tatcatgata
      901 agcagaccgg tcagctcatg tcgagagcaa taaacgacac tgcgaccttt gaggttttaa
      961 tagcccatgc cgttccggac cttattacca atttgcttat tcttatcgga accgggatac
     1021 ttttgtttgt aattagtccc ctgcttgcag tacttacgct tatacccatt ccctttttga
     1081 tactgggtgg tgccatattt acgaagaaga ttcttccaaa ctttcgggaa gcccaaggga
     1141 aactggggga actaaacgca gtgatgcagg acaatatttc gggcatgaaa gagatacagg
     1201 cctttaacca gcagtcgaga gagcgtaagc gtattaaaaa aggggccaaa aaatacactt
     1261 ctgcactgct tcacgcctta aagctcagtg ccgtgtttca tcctgcggtt gagatgataa
     1321 gttctctagg aacggttatt gtagtggcct ttggtggatg gctggcatta aggaactatg
     1381 tggacaccgc tgacatagtg ggctttataa tgtttcttgg gctgttttat cagcctataa
     1441 caactctggc aagggttata gaggatttgc agcaggcaac cgctggtgcc gaaagggttt
     1501 ttgaaatcat tgataccgag ccggacattg ttgacagtga aggtgctgta gagattgaaa
     1561 gagcaaaggg tgaaatatct tttaagaatg tgagctttca ctatatttcc caaagtccgg
     1621 ttttgaagaa tatcagtttt actgcaaagc ccggtcagat gatagcattg gtgggaccaa
     1681 ccggagtggg aaaaaccacg gttattagct tgatggcaag gttttatgac ccaacttcgg
     1741 gagaaatccg gattgatgat agaaatatta aggatgtgac ggtggcttcc ctgagaaacc
     1801 agatcagtat agtcctgcag gatgtattcc tttttaatgg ttcggtagcc gataatattg
     1861 cttacggaag cagggaggct tcttttgaag acattgtaaa cgcggctaaa atagcccggg
     1921 cccatgagtt tattactgaa atgtccgagg gctatgatac ggtaatcggg gaaagaggtg
     1981 taaagctttc aggaggacaa aaacagcgtc tgtccattgc aagggctgtt ttaagagaca
     2041 cgcccatact gatacttgat gaagcaaccg catcggttga cgttgagaca gaaacgcaga
     2101 ttcagcaagc aattggggaa ctggcaggca gccgtaccat tattgtaata gcccacaggc
     2161 tttctaccgt aaaacaggcc gataacatcc ttgtccttaa ggaaggagaa attgtcgagt
     2221 cgggcactca tgaagaactg ataaaactta acggcttata cagacatttg tgtgaagtgc
     2281 aatttggagg atagcctttg gacaatgttt ttgtttaaac tattgtttta atggtaaatt
     2341 atataggata caggccaaaa atttgtttta aaatgctaaa atattataaa taagaagtat
     2401 tgtattaaaa actaatgata tagtaggtga taaaatgtat tgcgttaaag aattaacaaa
     2461 agacttatac tgggttgggg gcagtgaccg cagactggca ttgtttgaaa atgtgtttcc
     2521 ggttcccagg ggagtatcat acaactccta tcttctaatg gatgaaaaga cggttttact
     2581 ggatacggtg gacaaggctg tgagcggaca attttttgaa aaccttgaat ttgtcctcgg
     2641 tgaaaggaaa ctggattatc tggtggttaa ccatatggag cctgaccact gcgggactat
     2701 gtgcgaactt gttctaaggt atccggatgt aaaaatagta ggaaatgata agaccgttgc
     2761 aatgataaag cagtttttta aatttgatat tgattccaga gcagtcatag taaaggagat
     2821 ggaaacgctt tgcacgggaa aacatactct tacctttata atggcaccta tggttcactg
     2881 gcctgaaaca atggtaacct atgatgttaa agaaaaggtg ctgttttccg gcgatgcttt
     2941 cggtaccttc ggtgccatta acggaaacat ttttgcagac gaggtggatt ttgagcacga
     3001 atggcttaag gatgcaagga gatattacac aaatattgta gggaagtatg gtgcacaggt
     3061 gcaaacctta cttaagaaag ctcaaaatat tgagatcaat atgatctgtc ctcttcacgg
     3121 tcctgtttgg aggacaaaca tagactggtt tgttgaaaaa tatcaaaagt ggagcacata
     3181 cacccctgaa gaaaactcaa ttatgattgc atatgcttcg gtctatggaa acacagaaaa
     3241 tgccgtcaat atacttgctt caaagcttgc tgatgcggga gtaaaaagga ttgcaatgta
     3301 tgatgtatcg gtgtcacacc cgtcagaaat tgttgcagaa gctttccggt gcagtcatct
     3361 ggtatttgct tccacaacct acaataccgg cgttttctgt aatatggaaa aggttctgct
     3421 ggacttaaag gcccacaatc ttcagaacag aacggtggct gttatagaaa acggtacctg
     3481 ggcggcaaca tcgggaaagc ttatgaaagg tatgatttct gaaatgaaaa acatgacaat
     3541 tctcgacagt actgtaagca tcaaatcttc actcaaagag aatcagattg aagaaataga
     3601 tgccattgct gccgctatat taaattcatt aaagactgca tagttcatat tgcttcattt
     3661 atgtaaactt cgattatgca tcagttggtg tgagggaacg aactttaaaa actctagttt
     3721 gacaagcaga gagcctgtaa gtactccgtg gaccgggata atccattttg catgaatggg
     3781 tgccatacct ttcaccttgt attgacaggc ttttcaactt atttcacgct gattcaccag
     3841 tatgattctt caaataacaa tatatgacag attcgaataa aaagcttgac tggaaaggga
     3901 cagtgatata ctatttattt aggtctgtct attcagaaaa atgtattgtc ggaggtaagg
     3961 caatgagtaa gagagttata ggaattgtgt ttatttgcat agtcttattt gttattgcgg
     4021 gatgcggggc aaaagagaac acccctgttc caaagacaga tgagatttca ggttactctt
     4081 ggaaggctac tgacgcttct gttttatatc ttgagtcaga cggtactttt aaatggtacc
     4141 ttcatgagaa aattagaagt gattcatatt atgtaggcac atatagtgtg tataacggac
     4201 aggaagcagt tgattatatg tcaacagaat taaaggaata caatgttacc tcccaaatgc
     4261 agatggatat gattgaatct gttgaagacg caagcctgga aaactattat tgtctggtac
     4321 ttaataacga aagatgtatt gttgatggaa aaaatacatt aagagataaa accgttgttc
     4381 cgtattatgg gttttattat gctgaggatg agtttttgga tttggtcaat ctggttaccg
     4441 aaaattatca tcaattcaca aaatatactg aataattgtt taatattaac catttaaatc
     4501 tttcaattta cgaaaataaa ccggctataa actttagccg gtttattttt ataaatcagt
     4561 atgtttcaac aaatacttca aagtatgatt gcgggtgaat gcatgccgga cacttatcag
     4621 gtgcttccgg accctcgtgt atataaccgc agtttccgca tttccaatag actttatcac
     4681 cttttttaaa tacagtgccg ttttcgatgt tagcggctaa tttattaaac cttacttcat
     4741 ggttaacttc agctttagca atcattctaa aggcagcagc aatttcagga aatccttctt
     4801 cgtcggcaac cttggcaaag gcaggataga gatcgctcca ttcctcattt tctccactgg
     4861 cagcagcttt gagattgtca agggtgctgc cctgagctac agggtaggat gcattaattt
     4921 caatcattgc aggtagttct ccctgaaatc cttctaataa aaatttataa aatctttttc
     4981 catgttcctt ttcattgtca gctgtttcaa taaaaatgtt tttaatttgt ttatatcctt
     5041 ctttgtcggc tacggacgcg tagaatgtgt aacggttgcg tgcttgagat tcgccggcaa
     5101 aggcttttaa tagattttca gctgttttag ttccctttaa attcatatca attcctccca
     5161 tatttttatt ttttattgtc aaggcatttt ggacagatgc ctttgtaata aacacttttt
     5221 tcattgattt tgaagctgct taaatcatcg gtttcaaaat ctacgctttt aatttgaaag
     5281 tcataaatag ttttacattt ttcgcactta aaatgcccat gatccgaaac atccccatca
     5341 tacctggttt cattttcttc tatagtgata acacggacaa tacttacacc gctaaacaga
     5401 ttcagggtat tatacactgt agttttcgac agagtgggca tttccttgac caactcatta
     5461 tatattttat caactgaagg atgatttttt tccgatagaa gatgttttaa aattgccacc
     5521 cggtggtatg acggctttat attatgtttt agcaaatata ctttaacctc ttctgttgat
     5581 aaattcattt taattctcct ttgacacaca aggtataaca acttgaaatt atttcgaatt
     5641 tgtaatgatt atcatgttgt attcattaca aatttattat aagttcaatt ttatatttcg
     5701 tcaatatata attaaaattt ccttatactt gttaagtatt cccttgaaat aaagtatatc
     5761 ttgcgtattt tactaaaata tgctatgcta aaatcgcttg atgtataatg atgtattatt
     5821 cagtacaaaa tacagtaaaa attaaagaaa gttgatggaa tatgggagtg acactaactg
     5881 attttattag ccaattatta tctaatcctg cattaattat tactacgctt cttacattgg
     5941 gtgtaatatt cgttaatggc tggacggatg ctccaaatgc cattgctact tgcgtttcca
     6001 cccgttcgat aggtccgcgt tctgctattg caatggcggc acttttcgat tttcttggtg
     6061 tcttttttat gacaatgttt aataagacag tagcagagac catttacaaa atggtggatt
     6121 tcggcgggga tccgaaagaa gctctggtag ccctttgtgc ggcacttatt gccatagtgc
     6181 tatgggcggt tacggcatgg tggttcggta ttccaaccag tgaaagccat gcacttattg
     6241 caggaatcag cggtgctgcg attgcccttc agagaggtat tggcggaatt aacttcgatc
     6301 aatggattaa tgttgtttac ggtttggcat tatccctcgt actgggattt ataatggggt
     6361 ggtcggcagt aaagacagtg gagttgattt tcagacgtgt taacaggacg aaaacctttt
     6421 ctcttttcaa gaatgctcaa attcttggaa gtgccggaat ggcatttatg catggtgctc
     6481 aggacggaca gaagtttatg ggagtttttt tactgggaat gtttttagct caagggcagg
     6541 caaatgttac ggaatttacg attccaacat ggctgatcat tctttgttcg acggttatgg
     6601 ccttgggtac ttccattggg ggctaccgca ttataaaggc agtgggaatg gatatggtta
     6661 agttggagaa atatcaaggc ttttctgcag accttgcagg agggatatgc ttgtttattg
     6721 cgtcgatttc aggattgccg gttagtacta ctcacacaaa gactacagct attatgggtg
     6781 tgggtgcggc aaagcgaata tcctcggtta attggggagt tgtaaaagaa atggtgactg
     6841 cctggatatt gacatttccg gggtgcggcc tgttgggttt tctaatggca ttattgttca
     6901 tgagaatatt ttaatgcata gttacaataa ttattaatga gagaggaatg aattatggca
     6961 agaaaaaaga atgaatacta ttatgatact tttgttgaat tagttgacta ttcatgtcag
     7021 gcagcaaatt tattaaacga tataatgaac aattttgatg cggcaaagtt acatgaaaaa
     7081 atggaagaaa tgcatgccat agaacatgcc ggggataaag caaggcatgt tatgataaaa
     7141 aaacttgtga aggaattcat tacacctatc gaaagagaag acatcatggc attggcagat
     7201 gcaatagaca atgtgacaga ctccattgag gatgtcgtta tgcgtatgta catgtttaat
     7261 gtcgtaaata ttcgcgaacg ggcgttggaa atgacgaagg tcgttgtcaa atgctgtaat
     7321 tcgttaaaac aggctttggc tgagttccat aatttccgca aatccaagac tctgcatcaa
     7381 ttgatcattg aagtcaacca actcgaagaa gagggcgaca ggctctttac tgaggcaaca
     7441 agggatttgt atgttaattg caacgatttt aaagaagtag cggcttggga cattgctttt
     7501 tactatcttg aaaaatgctg cgatgcttgt gaagatgttg cagacgctat tgagaacgtt
     7561 ataatgaaaa actcataagc ttttatccga actctaagac ctctaccttt tattaggcag
     7621 aggatttctt ttttaatttc agtggtggta agtactcatt gaagttagat ttacaataca
     7681 ccttttttat atcatgtcca taacagagag cttaatgtac gaatgatttg aagagtgaat
     7741 ttatactatt aattcagaaa caataaaaca aatagaaaat aaaaatatgg ttttggtgag
     7801 agagttatct tgtgaataaa atgaaaagaa gatgagtaat caccaaattc atattttttt
     7861 ctaatgagga gaaggcgaaa tgagtaatgg gattgatata tcagttgtag taccggcaca
     7921 taatgaagga aaatatgtaa agagatgtat agattctatt cgtgaatcag cggatgaatt
     7981 tgagggtaat gttgaaatta ttattgtttg taatagatgt actgatagaa cagaggatat
     8041 tgctaaagaa aatggagcaa tcacagttaa aaatgaagac agatgcattg caaaggttag
     8101 aaatgccgga atagatgcgg caagaggaaa gataataata acaattgatt gcgacaatcg
     8161 tatgacaaaa ggaactattt ctgaagttta tcatttgctt ggaagcggaa agtatatcgg
     8221 cggcggtgca ccgataagat ttgaaagata ttcatttccg ttgtggtgta acgatatttt
     8281 gtgtagattt tcatttgctg tcacaggatt atattgcggg ataatttggg cgacgaaaga
     8341 tgcgtttgat gcgattggcg gttttgtgga gaaaagggca atggaggatg tttcaactgc
     8401 aaagatgcta aagaagtatg gcaagtcaaa aggaatgaaa tacactacac ttaaaaagaa
     8461 ttatcttatt aattcaacaa gaaaatatga tgatttgggc gactggttat actttaagct
     8521 gatggtcaag aatgccggaa cacttataaa agcagcaatg ggtaatactc aagagtatga
     8581 taaattgatt gatgaaatgt tttacgatta caacgacaaa cattgacaga aagttctaaa
     8641 tgtatttttg tagaaagggc atgccaaatt ttacacaaag gaattcatta atctgaatcg
     8701 aataatattt tatgtaaacc gtcgtagaac tgacattata ctaaacatta ttctatcgaa
     8761 gggggattcg gttaatgaaa ctcaacaaaa aactgatttg tggggcttta actgttggat
     8821 tggtagcggg aagctcagtt tttgcatttg cggagagcat atcctataaa gtacagccgg
     8881 gagatgcatt ctggaagata agcagcaaat acggtatttc aacagttgaa ttgatgaaag
     8941 caaacaatgc cactgagaat acggtgttgt atccaggaca aatcataaca gtgcccttga
     9001 acaaccaagt ggtacatacg gttaagtcag gagatacgta ttggattatc agtcaaaaat
     9061 acggtgtgga tcttcaaaaa ctgttaagtg caaacaatgc caaccaaaac tcaattcttt
     9121 atgttggaga taaggtggtt ataccttccg ccaatcaaac atccgggtat aaaacctata
     9181 ctgtacaaaa aggtgacaca tactggacag taagccagaa atttaacgtc aatttcactg
     9241 agttattaaa aataaacgga gccaatgata agtcctacct agacatcgga cagattataa
     9301 aaataccgtc agcctcatca agtacaacat ctcctacaca accgggtgaa acctataagg
     9361 attatacggt tcaaaaaggg gataccttat ggagtatctc ggaaaagtat ggcataacag
     9421 taaatgattt agcgaaagca aataatataa gcacctcagc agtgcttaat atagggcaga
     9481 aattaaaagt tccggtgata aatgttccgg ttaagagcac tccaggtgcc caatacggcg
     9541 agcttttaga ttggtggacc gaagcacagt atgttattcc cagaggctgc acctttgagg
     9601 ttgtggactt ctatacagga aagtccttca tggcaaagcg tactgcaggt tcaaaccatg
     9661 ctgactgtga aacccttact gtaaaggata ccaatattat gaaggaaata tggggtggct
     9721 ttagctgggt gagaagacct gtgataataa agtataacgg aaggaaaatt gcagcgagca
     9781 tgacagcaat gcctcatgca ggaaatgata gtgcaccggg gggagcatgg gtatcctgga
     9841 gaagcggtga ttatggtgcg ggaacaaacc atgactacat taagggcaat ggaattgacg
     9901 gacactttga tatacatttt tataacagta ccaggcacaa ggacggaaaa gtggatacaa
     9961 accaccagaa ttgcattcaa atagctgccg gaaagaagta agataaaacc ggtgaaaaaa
    10021 tagtgcctaa atttcaaaaa aatagtctat ttacagaaaa caggaaataa agatttgaca
    10081 ggtatgggga agttattttg caaaattcag agggagcggt atattcctct gaattttgca
    10141 tgttaaaata cgctatgaga ttatacttaa taaatattga caatcgttcc gacagatacc
    10201 tggttgaaaa gttcgttgac atcctcatta aacattctga cgcaaccgtt cgatgcggct
    10261 gtgcctatgg aagcgggatt gttggttccg tggattccgt aatgcggaat tgacagtccc
    10321 atccacctgg ttccaaaagg acctccgggg ttaatctgct tgttaactat ggtaaaggtt
    10381 ccccgcggtg ttggcgttgt cggtttgcct gtcgctacgg gataagactt gacaagtctt
    10441 ccttcacggt acagggaaag ggtttttgcc gctatactta tagttatacg tgccggagat
    10501 ggggaagtgg gtatgcagat tacctgatcc acgtacagag cgtaagggtc aattccggga
    10561 tttgcattta taatctgttg tacggttaca ttaaagattc ctgcaatggt tgccagggtg
    10621 tcaccccttc gtataacata gtagtttaat gtgggacagg agggttccac tgcttttggc
    10681 tggggtatgc aaattacctg tccgatgtaa agcctttccg gaacaacact gggat
//
LOCUS       DAOOJC010000020        10653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_5601_length_10653_cov_3.661068, whole genome shotgun sequence.
ACCESSION   DAOOJC010000020 DAOOJC010000000
VERSION     DAOOJC010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 10653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10653
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5601_length_10653_cov_3.661068"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..594
                     /locus_tag="PLA01_01090"
     CDS             100..594
                     /locus_tag="PLA01_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 domain-containing protein"
                     /protein_id="HPC99925.1"
                     /translation="MIIDSKGKLFGKVSIIDILILAVLIVGIAGAAKFFKSKSTLPML
                     DKTETYTVGLYWEESPEFAVNAVKSGDIARDLERGGVLGKVTDIQIDKAITFVETEDG
                     RVVAGSKPGYVSYHITLEGTGSPDGYGGVVFNGADYQVGRQLIVKVGSAIFQGRIDRL
                     EKKG"
     gene            601..2211
                     /locus_tag="PLA01_01095"
     CDS             601..2211
                     /locus_tag="PLA01_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPC99926.1"
                     /translation="MLNNSLLLKPIFFVINIINTSYESSILKRLIDFIFGIFRKLNIL
                     YENSFTSYLARSVTGLFQNSAIVGFFIGKGRLSRWWEESLFYRIINGIVEIPSKLLKG
                     FYQKHEDFFLGSIIIRLVRELLSRLEIIVGLFLIILLVVPNARWNNVYNVGIALFLCA
                     LVFINTVIQRHWVFNFKALDFTLILFIVAVTLSLVTSLSFSASLKYWLFYLAGFLTVL
                     GIVSTVKNEGSLQTIVEMFLWGIAVIGAYGVWQAATGAVSFDPSLTDPDLNFNMPGRV
                     YATMANPNNYGEILIMALPFYISVVINSKTFVKRVVYAGLALFPLIALFYTGSRSSWI
                     GFAVSGIVITFLMNKRLLPFVFFGGMLLIPFLPQHVYNRILTIFKAAQDVKQDSSAQT
                     RVNIIKTVLPMFKTYIFSGVGLGTDIFRQIVKNYHQYTKAVPPHTHILYLQIWIEAGI
                     AGFITFIWYIYRTIKNSVIGVYNSTGPMKSIIAAGTASLAGILTTSLVEYSWYYPRVM
                     FIFWTVLGLLTAAVALTETKNRKLSENQ"
     gene            2280..2909
                     /locus_tag="PLA01_01100"
     CDS             2280..2909
                     /locus_tag="PLA01_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99927.1"
                     /translation="MFNLFKPKNPKNSFDWRVLKKNDISILILDERWNALFKNVEKTE
                     DIKEHETKIKELLKEESRLIAESKEIAAKKKKNLERIIQLTQQVYDDNSKEAKEEMKR
                     CELEVKQINERMKKITDELDNMPERIREANLRLLESTVRSVYFRMRKHQKRLKELERL
                     IETTKKQLEEYIDEKGRLSEDDTDVYTYFHDLLGGEELEKLDREYFGEA"
     gene            2973..4523
                     /locus_tag="PLA01_01105"
     CDS             2973..4523
                     /locus_tag="PLA01_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPC99928.1"
                     /translation="MKVVTPGQMREFDSYAINTVGIPGVVLMENAALKVVDEIMKDYG
                     GVKDMCICVFAGKGNNGGDAFAVARHLYNNGAKTVVFLLVSKKEISGDARINLEILDK
                     MGVPAEEVLDGDRLSEICESLEKAELIVDGIFGTGLKGRVEGYLAEIIRVINEKRTPV
                     IAIDIPSGINGETGDVCGECIRAYKTVTFGYPKLGHFLHPGCEFTGKLSVADISIPAD
                     VLERFEVKNEVIDRGMVIERLPGRNQNSNKGDYGRVLVITGSPGMTGAGCLSGSAALR
                     TGTGLLYLGVPNSLSGIYDCSLLEGVTIPLEDGGLGYLSKECVPKLMQHLEKMDAVVV
                     GPGLSTKEGVKDAVFSAIENSPVPLVIDADALNAVASDISVLQRSKTLKVLTPHPGEM
                     ARLLGITVSEVQGNRIKTAREFSEKWGVVTVLKGSRTIVASPDGRVYINPTGNSGMAT
                     AGSGDVLAGIIASLIGQGLDPVYAAVAAVYLHGLCGDRVAEVKGEHGMIAGDLVGELP
                     YVIKELLG"
     gene            4761..5930
                     /gene="alr"
                     /locus_tag="PLA01_01110"
     CDS             4761..5930
                     /gene="alr"
                     /locus_tag="PLA01_01110"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPC99929.1"
                     /translation="MEYKLNRAWAEVNLDNIAHNVREIRRITDKKTEIMGVVKADAYG
                     HGVMEVAKTLLENGVSRLAVSMLDEAIQLRQNGIGVPILILSYTDPVRAEEIVLNDVT
                     QTVFSHDLAEALSRAAVKHNRNVKIHIKIDTGMTRVGFMPGYSAVKNVVQISKLPGII
                     IEGLFTHFASADEADKSYTYMQFERFMSIINELNRIGVYIPVKHVCNSAGLIEFPEMH
                     LNMVRPGIVLYGLYPSHEVDETKLDLRPAMTLKANVILVKDVEPNTCISYGRIFQTSC
                     SSKIATIPIGYADGYTRLLSGKGKVLINGEFAPIVGRICMDQCMVDITDFKENVNVGD
                     EVVLFGRQKDKEIRVEDLAESIGTINYEFVCIIGKRIPRVYLKEGKVYNVLNYLI"
     gene            6258..6536
                     /locus_tag="PLA01_01115"
     CDS             6258..6536
                     /locus_tag="PLA01_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HPC99930.1"
                     /translation="MAQLKKILISLPDNLLKEVDSIVEMEKINRSEFVREAMKLYIRE
                     KRRIEMRDKLKKGYQQMAEINAKLAEICFEADNDQQQKYEEGLRELEK"
     gene            6536..6886
                     /locus_tag="PLA01_01120"
     CDS             6536..6886
                     /locus_tag="PLA01_01120"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPC99931.1"
                     /translation="MVIKRGDIFYADLSPVIGSEQGGIRPVLIVQNDIGNKYSPTVIA
                     SAITSQINKAKLPTHIELSAKDYGLPKDSVVLLEQIRTIDKKRLREKIGHLDDELMEK
                     VNEALTVSFGLIDM"
     gene            6907..7422
                     /locus_tag="PLA01_01125"
     CDS             6907..7422
                     /locus_tag="PLA01_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99932.1"
                     /translation="MKRSLKKSGILTLVIVLGIALVHIVQIVNAAATAEPEAEDDIIV
                     PSLDYVDEKINSLKTSLDEMTKQYEESQERLKEIESYGKFVPLELNKNQVLIAGESAE
                     IILRGGKALAIGGEGGGLSDITSGSGADVNTDQEVPLNHLLLISRDDGRGIKVVSEKA
                     WVLVKGPYTID"
     gene            7834..10290
                     /locus_tag="PLA01_01130"
     CDS             7834..10290
                     /locus_tag="PLA01_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPC99933.1"
                     /translation="MKSGFGNKLKNLGSKFRIVVNSIKDRVSGALRKKKDKGNAKSYH
                     EINASRGIKKDRNSALSRLVSFLRLGDIKIGVKITVSFILIIIVTLASAFFASMSSVM
                     KSMEEEAIFSTQTIVEQSAKSIKVLLEDVNGLAMLIATDEEIAQNVKQMNETDDPGEV
                     ARASSFIFPRMRTLKGTKSDKVMFIIASSLKGDVTVQGEGNSNDLGKSHYFDTIAYKE
                     FMRSGKNSYWVDTHFSDLIGQTAYSSEHNFCLFKVINTATSLKPVGYLQVNVNESTIG
                     NVLSEMKLPYNGDIYIVGQNWNMIYNRQNPNENAFRVNEINKINDQGQTKAEVLGRNE
                     LAKNDRALQEVLYRITDDEIGDNGFKQRDRYITDSIMERIYETIDSQGKISDDIKGEP
                     FKEIVNGEEMLVTYYTIRSIKGTQLEWSIISLTPVSEITSQVNSAVAWIILIGILCFI
                     FAALIAILITYDISSGVKVLSKFMGSIQKGNLDIEYNLKRKDEIGALGINFRDMVVNL
                     RTLIGSIKGASNVAIESSQTVSSTSEESYASIQEFLTLLTEVNKEIGMQKTEIDNNEK
                     IAQSLSDKIQIITKDFGNVNEIIFGAKELGEKGKDTVNSLQQNAQQVKVTINEFSELI
                     NVLRTESGEISKITEAIKGIAKQTNLLALNATIEAARAGEAGKGFAVVAHAIKKLADQ
                     SRESASFIERKLKLITNTIEETGKVVKVSDNVISKHDLAVHDTIEKFDNIVDFMDDVY
                     NQVRSINNSIMSIDEARVDIMESLRNMNESSRKNVGSINEITVGFNELVDLIKHLVSL
                     SDDLRKLSKNLESSIDMFKV"
     gene            complement(10371..>10653)
                     /locus_tag="PLA01_01135"
     CDS             complement(10371..>10653)
                     /locus_tag="PLA01_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HPC99934.1"
                     /translation="SGADVLLVALGAPRQEKCIHKNRDKLKVKICIGVGGTFDVLAGR
                     AARAPKFFQKNGLEWLYRLCKEPWRFIRMMDLPRFILLVMAVKIGLKKA"
BASE COUNT         3409 a         1680 c         2576 g         2988 t
ORIGIN      
        1 tgcctatgcc ttgcaggtat gactttcaac cataccgggc aaagacggta ttagaaaaga
       61 ttggaatata tacaaattat aacagggagt gaagaaaaca tgataattga cagtaaagga
      121 aagttgtttg gaaaggttag tattattgat attcttattt tggcggtatt gattgtagga
      181 attgcaggtg cagcaaagtt ttttaagtcg aaatccacct tgccgatgct tgacaaaacc
      241 gaaacttata cggttggatt atattgggag gagtctcccg aatttgcggt aaatgccgtg
      301 aagtccggcg atatagcaag agaccttgaa aggggaggcg ttttgggaaa ggttacagat
      361 attcagatcg ataaggctat aacctttgtt gagaccgaag atggcagggt tgtggccggt
      421 tcaaagccgg gttatgtatc ctaccacatt acccttgagg gcaccggttc accggacggc
      481 tatggaggag tagtttttaa cggagcggat tatcaggtgg gaaggcaatt gattgtaaag
      541 gtaggaagtg ctattttcca aggaaggatt gacagattag agaaaaaggg gtaatgcgtt
      601 ttgttaaata atagtttgct gttaaagcca attttctttg ttattaatat aattaacact
      661 tcctatgaga gcagtatttt aaaaaggctg attgatttta ttttcggtat tttcaggaag
      721 ctgaatattt tatatgaaaa cagttttact tcatatttgg ccaggtctgt aactggactt
      781 tttcaaaaca gtgctatagt tggctttttt attggaaaag gaaggctttc ccgttggtgg
      841 gaagaaagcc ttttttacag gataataaac ggtattgtag aaataccttc aaagcttttg
      901 aaggggtttt accaaaaaca tgaggatttc tttcttggaa gcattataat aaggcttgta
      961 agagaattgt tgtcaaggct tgaaattata gtgggcctgt ttctgataat tttacttgtg
     1021 gtgcccaatg cgagatggaa taatgtctat aatgtaggta ttgctttgtt tctttgtgct
     1081 ttggtcttta taaacaccgt tattcagagg cattgggtgt tcaactttaa ggcattggat
     1141 tttaccctta tactgtttat tgtggcggtt acgctgtcct tagttacttc attgagtttt
     1201 tccgccagtc ttaaatactg gttgttttat ctggcgggtt ttttgacggt gctggggata
     1261 gtcagtactg ttaaaaatga gggttctctt caaacaattg tggaaatgtt tctgtgggga
     1321 atagcagtaa tcggggccta tggagtttgg caggcggcca cgggagcggt gagctttgat
     1381 ccttccctca cagacccgga cttgaatttc aatatgcccg gaagggtata cgcaaccatg
     1441 gcaaatccga ataactatgg agaaatactt atcatggcat tgccctttta tataagtgta
     1501 gttataaact caaaaacctt tgtaaaaagg gtggtatatg cgggattagc tctttttccg
     1561 ttgattgcac ttttttatac cggctcaagg tcgtcatgga taggatttgc agtttctggt
     1621 attgtaataa cgtttttgat gaacaagagg ttgctgcctt ttgtattttt tggagggatg
     1681 cttctcattc cgtttctgcc gcagcatgtt tataacagaa tcctgactat ttttaaggct
     1741 gcacaggacg ttaagcagga ttcttcggca cagacaagag tcaatataat aaagaccgta
     1801 ttgccaatgt tcaaaactta tattttttca ggagtgggct tgggcacgga cattttccga
     1861 cagatagtca aaaactatca tcagtatacc aaagcagttc cgccccacac tcacattctt
     1921 tacctgcaga tctggataga ggcaggtatt gccggattca taacttttat ctggtacatt
     1981 tacaggacaa taaagaactc ggtcattggt gtatataatt caaccggacc catgaaaagt
     2041 ataattgcag caggaaccgc atcccttgcc ggaatattga ccacttcttt agtggaatac
     2101 agctggtact acccaagggt tatgtttata ttctggacgg ttttgggatt gttaaccgct
     2161 gcagtggcat tgacagagac aaaaaacaga aaattatcgg aaaaccagta acaaaatcat
     2221 gcaattattt cgatacaaag aatagggggt agtaacacat tgcatgaggg gttggttgca
     2281 tgtttaattt atttaaacca aagaacccaa agaatagttt tgattggaga gtactgaaga
     2341 agaatgatat ttcaatattg atattggatg agcggtggaa tgccctcttt aaaaatgttg
     2401 aaaaaactga ggatatcaag gagcatgaga caaaaattaa agagctttta aaggaagagt
     2461 cgcgtcttat tgccgaatct aaggagattg cagctaaaaa gaaaaagaat cttgaaagaa
     2521 ttatacagct cactcagcag gtttatgacg ataatagcaa agaagcaaag gaagagatga
     2581 aaagatgcga acttgaagtc aaacaaataa atgaacgtat gaagaaaatc accgatgaac
     2641 ttgacaatat gcccgagaga atacgggagg caaaccttag gctgttggaa tctactgtca
     2701 gatcggtgta ttttcgaatg aggaagcatc aaaaaaggct gaaggaattg gaaagactaa
     2761 ttgagacaac aaaaaagcag ctcgaagaat atattgatga aaaaggaaga ctgtcggagg
     2821 acgataccga tgtctatacg tattttcacg accttctcgg aggagaagaa ttggaaaagc
     2881 tggacaggga atacttcggg gaggcatagt ttaagatgtg atgattattc ttccccatgc
     2941 ttttgtacct cgagaaagga aggacacgta aaatgaaggt tgttacaccc gggcaaatga
     3001 gggaatttga ttcatatgcc attaataccg taggaattcc cggtgtggta ctgatggaaa
     3061 acgctgcttt aaaagtggtg gacgaaatca tgaaggacta cggcggcgta aaggacatgt
     3121 gcatatgtgt gtttgcagga aagggcaata acggtggaga tgcttttgca gtggcaaggc
     3181 atctttataa taatggtgcg aagacggttg tttttttact tgtctccaaa aaagaaatat
     3241 ccggggatgc ccgtataaac cttgaaatac tggataagat gggagtacct gcagaggagg
     3301 ttttggacgg agacaggctt tcggaaatat gtgaaagcct tgaaaaagca gagcttattg
     3361 tggacggcat atttggaaca ggcttaaaag gcagggttga aggttattta gcagaaatta
     3421 taagggttat caacgaaaag agaaccccgg tgatagccat agacatacct tccgggatta
     3481 acggcgagac gggagatgtg tgcggtgagt gtatccgtgc gtataagaca gtgaccttcg
     3541 gttatccgaa gctggggcat tttttacatc ccggctgtga gtttaccggc aaattgtctg
     3601 ttgcagacat aagtataccc gcagatgtgt tggaaaggtt tgaggtaaaa aatgaagtca
     3661 tagacagagg catggttata gaaaggctgc cgggaagaaa tcaaaacagc aacaaaggcg
     3721 attacggcag ggtacttgtt ataaccggat ccccggggat gacgggggca ggatgccttt
     3781 cgggatctgc cgcactgcga accggaacgg gtcttttata tcttggagtt ccgaattccc
     3841 tgtcgggtat atatgattgc agccttttgg aaggagtgac aattcccctt gaagacgggg
     3901 gcttgggcta tctgtccaag gagtgcgtac cgaaacttat gcagcatttg gaaaagatgg
     3961 acgcggtggt tgttggaccg ggcttatcca caaaagaggg tgtgaaggat gcggttttca
     4021 gtgctatcga gaacagccct gtgccgctgg taattgatgc cgatgcttta aacgcggtgg
     4081 cttctgacat atcagtattg caaaggagca aaacactaaa ggttttgact cctcatcccg
     4141 gtgagatggc aaggctttta ggcatcaccg tttcggaggt tcagggcaac agaataaaga
     4201 ctgcccggga attttcggag aaatggggtg ttgtaaccgt tctcaagggt tcaagaacca
     4261 ttgtagcctc acccgacggg agggtgtata tcaaccccac aggaaattcc gggatggcaa
     4321 ccgcaggaag tggagatgta ctggccggta ttattgcaag tcttataggg cagggacttg
     4381 accctgttta tgcggcagtt gcagctgtat atttgcatgg actttgcggt gaccgtgttg
     4441 cagaagtaaa aggggagcat ggcatgatag ccggtgactt ggtaggggag cttccctatg
     4501 ttatcaaaga attgcttggt tagggtgtat aatatctgtt aagaaactta tggaagaaac
     4561 attgtgaagc aagttgtaga gcctgttaat atgacgcgga aagggagagc attgtttgag
     4621 cgaagcgagt tttgctcgac cccgcggagt atttacaggc tctagactgc gtaacttaag
     4681 tttctggaat atgtttctta acgaattaat ctgcacctgc aaaagttaat aattttatat
     4741 tcataaaaga gggagttaac atggaataca agctaaacag ggcatgggcc gaggttaatc
     4801 tggacaatat tgcccataat gtaagggaaa taagaaggat tacagataaa aagaccgaaa
     4861 taatgggagt ggtcaaggct gatgcctatg gacacggtgt tatggaggtt gcaaagacac
     4921 tgctggaaaa tggagtgagc cgccttgcgg tttctatgct ggatgaagcc atacagcttc
     4981 gacagaacgg tatcggagtt cctatactta tactgagcta taccgatcct gtgcgggcgg
     5041 aggaaattgt tttaaatgac gtcacacaaa cggttttcag ccatgacttg gcagaggccc
     5101 tttcgagggc agcggtaaag cataaccgta atgtaaagat tcatataaag atcgacacag
     5161 gaatgacaag ggttgggttt atgccgggat acagtgcggt aaaaaatgtg gttcagataa
     5221 gcaagcttcc gggtattatt atcgaaggac tgtttacgca ttttgcatca gcggatgagg
     5281 ccgacaaaag ctatacctat atgcagtttg aaaggtttat gagcataatt aacgagttaa
     5341 acaggatagg agtttatatt cccgtcaagc atgtgtgcaa cagtgccggt ttaattgagt
     5401 ttcctgaaat gcaccttaat atggtaaggc cgggaatagt gttgtacggg ctgtatcctt
     5461 cccatgaggt ggatgaaaca aagcttgatt taagacctgc aatgacattg aaggctaatg
     5521 tcattctggt gaaggatgtg gaaccgaata catgtataag ttacgggcgg atattccaaa
     5581 cctcgtgcag cagcaagatt gctaccatac cgataggata tgcggatgga tatacaagac
     5641 ttttgagcgg caaagggaaa gtcctcatta acggtgaatt cgccccaatt gtcgggagaa
     5701 tatgtatgga tcaatgcatg gtggatataa ccgactttaa ggaaaatgtg aatgtcggcg
     5761 atgaagtggt actgtttgga agacagaagg ataaagaaat aagggttgag gacctggcag
     5821 aaagtatcgg cacaataaac tatgagtttg tatgcattat cggtaagaga attcccaggg
     5881 tatatttaaa agaaggaaaa gtatataatg tattgaacta tttaatataa tgaggcaata
     5941 gatatctcca gcttttttag cagacgacaa attctctttg aactgaagtg ctaaaataaa
     6001 ctaaaattaa tgtataactt ttggcaaata ataaaaaact atactagaaa tagagggata
     6061 ttagtattta attgcatata ggcaaaattt acatgtgctg ctttgaaggg ggagtaatta
     6121 aaatttaatt tgacctatgc atatattgaa ctttataatt atatatataa gtatagaatt
     6181 gcaatctcag tggttaccga tttagccttt attaattctt tatgtcttaa cccaaaaaaa
     6241 gaagtggggg ttttactttg gcacaattaa agaaaatatt aataagtctt cctgacaact
     6301 tgctaaaaga ggttgattcc atagtagaaa tggagaaaat taacagaagt gagtttgtaa
     6361 gggaagcaat gaagctttat atacgagaga aaagaagaat tgaaatgagg gataaattga
     6421 aaaaaggtta ccagcaaatg gctgaaatta atgctaaact tgcagaaatt tgttttgaag
     6481 cagacaacga tcagcagcaa aaatatgagg aaggactcag ggagttggaa aaatagtggt
     6541 aattaagcgt ggagatatat tttatgccga tttaagtccg gttatcggct ctgaacaggg
     6601 aggaatcaga cctgtattga ttgtccagaa cgatattgga aataaataca gtccgacggt
     6661 catagcatca gctataacat cgcaaataaa caaagcaaaa ctgcctaccc atattgaact
     6721 cagtgcaaag gattatgggc ttccaaagga ttcggtggtg cttttagagc aaatacggac
     6781 catagacaag aaaagattga gggaaaaaat cggacatttg gatgatgaac tgatggagaa
     6841 ggttaacgaa gctttgacgg taagctttgg tttgatagat atgtaattta tggagggata
     6901 caaaagatga agaggtcatt aaaaaaatca ggaatactta ctttggttat tgttttaggt
     6961 attgctttgg tacatattgt tcagattgtt aatgctgctg ctacggcaga gccagaggct
     7021 gaggatgata ttattgttcc ttctctagac tatgtggatg aaaagataaa cagtcttaag
     7081 accagccttg atgagatgac aaagcaatat gaggaatctc aagaacggct taaagaaatt
     7141 gaaagctacg gaaagtttgt gccgttggaa ctgaataaga atcaggttct tattgccggc
     7201 gaaagtgcag agattatttt aaggggcggt aaagcactgg caataggcgg agaaggcgga
     7261 ggactttcag acatcacatc gggttcgggt gcggatgtaa atacggatca ggaagttcct
     7321 ttaaaccacc tgcttttgat atcaagggat gacggcaggg gcattaaagt tgtgagtgag
     7381 aaagcctggg tgcttgtaaa gggaccctat accatcgatt agtgcagttg ccataggttg
     7441 ttagaacgtg cttcttttct tatatatatt tattttagag aaggaaattt cacttttata
     7501 gagaataata tatttaagga caaaattgtt attgtcttta tttgcatttt tttgcttatt
     7561 aacatttcaa ataccggctt cgtttaaatg ctgtcaatac ttttgtttta tactgaaaac
     7621 tcaggaattt cgattttgta atggatttat gaaaggatgc aattgtatgc atctgaatta
     7681 ttaaagaatt cattcagtta gaatctataa ttaaaagcaa ttccttaaga ttatatttat
     7741 cgcaagactt tggtaaatca ccttcagttg tattcagcaa tttttgtttg ctatgttacc
     7801 ggtcttatgg ctggaatttg aagaggtgaa ggtatgaagt caggtttcgg aaataagctt
     7861 aagaaccttg gcagtaaatt ccgaattgtg gttaattcaa taaaagacag ggtctcaggg
     7921 gctttaagaa aaaagaagga caagggcaat gctaaatcat atcatgagat taatgcttcc
     7981 agaggcataa aaaaggatag aaactccgct ttaagcagac ttgtgagttt tctaaggctg
     8041 ggcgacataa agattggggt taaaataact gtttctttta tacttataat tattgttaca
     8101 ctggcctcag cgttttttgc ttccatgagc agtgttatga agagtatgga agaagaagcg
     8161 atatttagca cgcagacaat tgtggagcag tctgccaaaa gtataaaggt attgctggag
     8221 gatgtgaacg gacttgccat gcttatagca acggacgaag aaatcgccca aaatgttaag
     8281 caaatgaatg aaacagatga tcctggtgaa gtggcaagag cttcaagttt tatttttccc
     8341 cgaatgagaa cattaaaagg gacaaaatcg gataaggtta tgtttataat agcatcaagt
     8401 cttaagggcg atgttaccgt gcagggagaa ggaaattcaa atgatttggg aaagagccat
     8461 tactttgata ctattgcata taaagagttt atgaggagcg gaaagaattc ctactgggtt
     8521 gatacccatt tttccgatct catcggtcag actgcttata gttcggaaca taatttctgc
     8581 ttgtttaaag tgattaatac agctacaagt cttaaacctg ttggttattt acaggttaac
     8641 gtgaatgaat ccactattgg aaatgttctt tcagaaatga agctgcccta taatggtgat
     8701 atttacattg taggacaaaa ctggaatatg atatacaacc gtcagaatcc caatgaaaat
     8761 gcttttcgag taaatgagat aaacaagatt aatgaccagg ggcagactaa agcggaggtt
     8821 ctaggaagga acgaattagc taaaaatgac agagctcttc aagaagtatt atatagaata
     8881 actgatgacg aaataggcga taatgggttt aaacaaagag accgttatat tactgattct
     8941 ataatggaaa gaatatatga aactatagac tcgcagggaa aaataagtga cgatataaag
     9001 ggagagcctt ttaaggagat tgtaaacgga gaagaaatgc tggttaccta ttatactata
     9061 cgatcaataa agggaacaca gctagaatgg tcaataattt cattaactcc cgtcagtgaa
     9121 attacatctc aggttaatag tgcggttgca tggataattt taattggtat tttgtgcttt
     9181 atttttgcag cacttattgc tattttaata acttatgaca tatcatccgg ggttaaagta
     9241 ttgtctaaat tcatgggttc aatccaaaag ggtaacctgg atatagagta taatcttaaa
     9301 cgaaaggatg agataggtgc tctcggtatt aacttcagag acatggtggt taatctgaga
     9361 acacttattg gaagtatcaa aggggcctcg aatgtagcta tcgaatcgtc ccagacagtt
     9421 tcaagcactt cggaagagag ctatgcctcc atacaggagt ttttaactct tttgactgaa
     9481 gtaaacaagg aaatcggaat gcaaaagacg gaaattgaca ataacgaaaa aattgcacag
     9541 agcctttccg ataagattca gatcataacg aaggatttcg gcaatgtcaa tgaaataata
     9601 ttcggggcaa aagaactcgg tgaaaagggt aaagatacgg taaacagcct ccagcagaat
     9661 gcacaacagg ttaaggttac cattaatgaa ttttctgagc ttataaatgt attgagaaca
     9721 gagtcgggtg agatatctaa gataacggaa gcaattaagg gcatcgcaaa acagacaaat
     9781 cttctcgcat tgaatgctac catagaagca gccagagcgg gagaggctgg taaaggcttt
     9841 gctgttgttg cccatgccat aaaaaaactg gccgatcaat cccgagagtc tgcctccttt
     9901 attgaacgaa aactaaagct cattaccaat acaatagagg aaaccggaaa agttgtaaaa
     9961 gtatccgata atgttatatc caagcatgac ttggctgttc atgacacaat agagaagttt
    10021 gataatattg ttgactttat ggacgatgtc tataatcagg tgagaagtat aaacaattca
    10081 ataatgtcta ttgacgaagc cagagttgat attatggaat cgttgaggaa catgaatgaa
    10141 agctcaagga aaaacgtcgg aagcataaat gaaataactg tcggatttaa tgaactggtt
    10201 gatttgatta agcatctggt ttcactgtcc gacgatttga gaaagctttc aaagaatctt
    10261 gaaagttcca ttgacatgtt caaggtgtaa ggtgaagaag aatactccag aaactaaagt
    10321 tacgcaagta aagagcctgt aaatacgaag aatattcttc ttcacttctc tcaggctttc
    10381 ttcaatccga ttttaacagc catgaccaga agaataaaac ggggtaagtc catcattctg
    10441 ataaaacgcc agggttcttt gcagagcctg tataaccact cgagaccgtt cttttgaaag
    10501 aatttcgggg cgcgggcagc ccggcctgcc aatacgtcaa aagtacctcc tacgccgata
    10561 catattttta ctttcagctt atccctgttc ttgtgtatac atttctcctg cctgggtgca
    10621 ccgagggcaa caagcagaac atcggcaccg gat
//
LOCUS       DAOOJC010000021        10638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_5611_length_10638_cov_3.620618, whole genome shotgun sequence.
ACCESSION   DAOOJC010000021 DAOOJC010000000
VERSION     DAOOJC010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 10638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10638
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5611_length_10638_cov_3.620618"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /gene="ylbJ"
                     /locus_tag="PLA01_01140"
     CDS             <1..1114
                     /gene="ylbJ"
                     /locus_tag="PLA01_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HPC99935.1"
                     /translation="IQLWLGVVFPSLFPFFVVSEILNKTRFINAIGVLLEPVMRPLFN
                     VPGCGSFALAMGITSGYPVGAKITAGMREENLLTKTESERLLSFTNNSGPLFIVGAVA
                     VGMFKMPELGVLLLLCHMLASITVGILFRFYGRNNESTRTVSNEKLLVKFKKELSKSS
                     RQNINLGIILTESIKNSVSVMLAIGGFIILFSVIINVLIDIGFISSLSDLIYAVLSLA
                     GIPKEMITALVSGFFEITTGTNMACNVGGIALIDKLAAVSLILGWAGISVHSQVYSII
                     SKTDISIKPYLFGKMLHGIFAAVYMSIAARLPHTAPLMVKSVFNERVMQSEFPWINLF
                     LYSLKNMLFSLAVIIVFIVTSLIFSVIKELVCPKKF"
     gene            complement(1126..1563)
                     /locus_tag="PLA01_01145"
     CDS             complement(1126..1563)
                     /locus_tag="PLA01_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPC99936.1"
                     /translation="MEMLSILETLEDLIEKSISVPFSGKCMVDKEEILEIIKEIRLKL
                     PDDIKQAKWVKEERQRILLEAQKEANNIIKDAENQISALVDEHEITKKAYEQSNEIIT
                     NAQKNAREIRLGTKEYADGILNKVEEILEETLQVIKENRKELK"
     gene            complement(1764..2243)
                     /gene="coaD"
                     /locus_tag="PLA01_01150"
     CDS             complement(1764..2243)
                     /gene="coaD"
                     /locus_tag="PLA01_01150"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPC99937.1"
                     /translation="MSVYVYPGSFDPITNGHMDIIERASKLCDKLIVAVLVNRSKKPV
                     FSLEERVDLIKCSVKNIHNVEVESFSGLLIDYMRNKNAKVIIKGLRAVSDFEYELQMA
                     LLNKNLDSGIETLFMMTNIHYSFLSSSSVRELAEHKGKIDGLVPECIRDRVIKRFAQ"
     gene            complement(2269..2847)
                     /gene="rsmD"
                     /locus_tag="PLA01_01155"
     CDS             complement(2269..2847)
                     /gene="rsmD"
                     /locus_tag="PLA01_01155"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPC99938.1"
                     /translation="MEEYVILRVISGTAKGHKLKTLKGISTRPTSDRVKESLFNIISG
                     MIPGAGVLDIYAGTGSIGIEALSRGADFAVFVDKSNECSRIIRENLVNTKLIDKASVI
                     TGDIFITLNKISKNNKKFDIIFLDPPYKKNLVVETLKCIEKNAIIKPEGIIIAEHSFD
                     DGIQEEIGGYKRYRCQKYGITAISFFRGMDDR"
     gene            complement(2933..5017)
                     /gene="recG"
                     /locus_tag="PLA01_01160"
     CDS             complement(2933..5017)
                     /gene="recG"
                     /locus_tag="PLA01_01160"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPC99939.1"
                     /translation="MNSGIEDILKKSIQYIKGVAEKRSKLFHRLGVFSVCDLITFFPR
                     GYEDRSKLKKISQMENGEECTFEGIVMSKVSEKRIRKGLSIYQVYVKDDTGTVIATWF
                     NQPFVKKIFNVGECYVFFGKVTGKYKYLEVNNPVYERVDTGEYKNTMRILPVYPSTAG
                     LSQNTIRTVIENALEIVAGMLEDPLPGWIRKEYRLCDINYSFSNIHFPRSEEDLNYSR
                     YRLVFEELFILQLGLLSIKSALGDGKEGICFGPHEKELEHFIANTGFELTNAQKRVWR
                     EIEEDMESPQIMNRLVQGDVGSGKTVIAALALLKAVKSGYQGIMMVPTEILAKQHYET
                     LCSLMEKSGVSISLLVGSQTAKQKKELLSRIEAGEVDIVIGTHALLEDNVKFCRLGLV
                     ITDEQHRFGVRQRAVLSGKGENPDVLVMTATPIPRTLALILYGDLDISVIDELPSGRK
                     PIKTYAVDNSKRDRINSFIRKNISEGHQVYIVCPLVEESDAIEAKSALEMAEKITKED
                     FNDLRVGLVHGKMPPLEKDKVMQRFADGEIDILVSTTVIEVGINVPNATVMVVENAER
                     FGLAQLHQLRGRVGRGGYQSYCILYNESKSQIARERMKVMENSTDGFVISEKDLEIRG
                     PGDFFGTRQHGLPELKIANLYRDMDILKKAQEAANEVMRRDRLLCEEENIKLKEKIVE
                     KFKGKLKDISLN"
     gene            5343..5531
                     /gene="rpmB"
                     /locus_tag="PLA01_01165"
     CDS             5343..5531
                     /gene="rpmB"
                     /locus_tag="PLA01_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPC99940.1"
                     /translation="MAKCEICSKDVLFGIKVSHSHRRTNRSWKPNVRKVRIVDNGTPK
                     TVNICSRCLRSNKVTRAI"
     gene            complement(5923..6186)
                     /locus_tag="PLA01_01170"
     CDS             complement(5923..6186)
                     /locus_tag="PLA01_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase"
                     /protein_id="HPC99941.1"
                     /translation="MGGFIVTVLIAVLVGWLGNAISPDKIPGDAWGASLVALIGAWIG
                     AYMPYFNVFGPGAMEIALVPAFLGAIIATVVFGAVSRVIKQAS"
     gene            complement(6505..8187)
                     /locus_tag="PLA01_01175"
     CDS             complement(6505..8187)
                     /locus_tag="PLA01_01175"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPC99942.1"
                     /translation="MNRIGKYELMLLGITWLIIGTIAAHVITVDAGTLANWNICPGVV
                     KVCFLLGMIIFSLIKAVFAWKDFFGSNKFYMPIKLLEVLFIHIAMVFIGAGEWANYIL
                     LFLVMVTALCVGRKFSFILVGYSLLLNIVMRIVNNLYFLEDGFAVTDEIVKEFPYIIS
                     VHLVMFLLAVLCSWFYSDNNKNEQQNRKLFMELANRYDQVAAAQEEIKVQNGKLKDAN
                     CKLEETNKKLMTSLAELFTLQQITQAISSILDIDDLLKDVNDIIIGVMGVSSSTIIRY
                     DDKKCRLKVHTTNVSKRNGLITLNDNINCDKLMDVLETGDPVIENCVNANDFPFTEGR
                     GIKSLICVPLVTKSRKFGLVLVEHMYSNAFDENNLRLLNIIGQQVGIAMENVELYQRM
                     HELATVDGLTGVYNRLYFQERLKEEFESAKKEKYDLSLALLDIDHFKKFNDTFGHLFG
                     DKVLSRIATLIKNSLRSDDIIARYGGEEFVILLPRTGINEAYYKVERLRDLISKTSIT
                     DGKATASVTVSFGVSSYPEFSSSEAELLKMADDALYDAKESGRNCVKITGGN"
     gene            complement(8272..8910)
                     /gene="nth"
                     /locus_tag="PLA01_01180"
     CDS             complement(8272..8910)
                     /gene="nth"
                     /locus_tag="PLA01_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPC99943.1"
                     /translation="MDKKERVESIIKVFKKIYKDATCTLDYKDPLQLLVATQLAAQCT
                     DVRVNMVTNTLFKKYKTALDFANADLEELERDIMPTGLYRNKARNIKEASRIIVDQFN
                     GQVPGNLEDLLTLPGVGRKTANLVLGDVFGIPGIVVDTHAKRLSNRIGLSSSNDPKKV
                     EFDLMAVVPKENWSEFCHQLVYHGRAVCHARKPECNKCSIIDYCDYGRQFLK"
     gene            complement(9094..10557)
                     /gene="glgA"
                     /locus_tag="PLA01_01185"
     CDS             complement(9094..10557)
                     /gene="glgA"
                     /locus_tag="PLA01_01185"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HPC99944.1"
                     /translation="MRGNDSLKVLFVSVEVAPFAKTGGLADVAGSLPKSLVSMGYDVR
                     IAMPRYQQINADMSYVTDFPVEMGNRQATCIVREGSIDFRDKEKDSKVPVYFVDNYHY
                     FNRGGIYCYFDDADRFAFFCKAVLEMLPKIDFKPDIIHCNDWHTGPICMFLKENYKSY
                     PYYKKIKTLFTVHNLEYQGNFSKDVLGIMGIPDELFVPEKVEFYGMFSFMKAGLVYAD
                     IISTVSETYAKEIQTERYGEKLEGLLKNRSKDLYGIVNGIDYDVFNPETDPRINKNYN
                     VETFKLKVENKYALQKEMGLPKKDVPAIGLISRLTGQKGLNLIMDEIDEMMKDDIQFI
                     LLGAGDEYYETGFKKIQEKYPEKVGVYIGFNVPLAQRIYAGCDMFLMPSRFEPCGLGQ
                     LFSLRYGTIPIVRATGGLAETVIDVDVDRKNGNGFSYTEFTSKDMLKTLKRAIKFYNE
                     KPQEWDMIVKRAMSADYSWNKSAGKYSDLYAKLMKKK"
BASE COUNT         3095 a         2424 c         1652 g         3467 t
ORIGIN      
        1 aattcagcta tggcttggtg ttgtgttccc ttctctgttt ccgttttttg ttgtgtccga
       61 aatccttaat aagacaagat tcataaatgc cataggagta cttttagagc ctgtcatgcg
      121 tcccttgttc aatgtgccag gatgcggttc ctttgctctt gcgatgggaa ttaccagcgg
      181 ttatccggtg ggagcaaaaa tcactgccgg tatgcgagag gaaaatctcc tcacaaaaac
      241 cgagtctgaa aggcttttat ccttcaccaa taattccgga ccgctgttta tcgtcggtgc
      301 cgttgcggta ggaatgttta aaatgccgga actgggagtt ttacttctcc tatgccacat
      361 gctggcaagc atcactgtgg gaattctttt cagattctat ggcaggaata atgagagtac
      421 acgtactgtg agcaacgaaa aattactggt aaaatttaaa aaagaactgt ctaaaagcag
      481 caggcaaaac ataaacctcg gaataatact gacagaatca attaaaaact cagtcagtgt
      541 aatgctggcc atcggaggct tcatcattct gttttcagtt atcattaatg ttttgattga
      601 tattgggttt atatcctctc tgtccgactt gatatacgca gttctatccc ttgcgggtat
      661 acccaaagaa atgattaccg cattggtaag cggctttttt gaaataacta ccggaacaaa
      721 catggcctgc aatgttgggg gtattgcact tattgacaaa cttgccgcag taagccttat
      781 actcggttgg gccggtattt cagtacattc acaggtttac agcatcataa gcaaaaccga
      841 catcagtata aaaccttatc tttttggcaa aatgcttcac ggaatattcg ctgcagtata
      901 catgtcgata gcagcaagat tgccccacac agcccctctc atggtcaaaa gtgtatttaa
      961 tgaaagggta atgcaatcgg aattcccatg gattaatttg tttctttatt cgttgaaaaa
     1021 tatgttgttt tcccttgccg ttattattgt attcatcgtc acatcgctta ttttcagcgt
     1081 tattaaggaa cttgtatgtc caaaaaagtt ttaaccgcca tgcaattatt taagttcttt
     1141 acggttttcc tttattactt gcaacgtctc ctcaaggatt tcctcaactt tgtttaaaat
     1201 gccatcagca tattcctttg taccgagcct gatctccctg gcatttttct gtgcatttgt
     1261 aattatctcg tttgactgct cataggcttt tttggtaatc tcatgttcat ccacaagggc
     1321 tgaaatctgg ttttctgcat cctttattat attattggct tccttttgtg cctcaaggag
     1381 aatcctctgt ctttcctcct taacccactt cgcctgtttg atgtcatcag gcagtttcaa
     1441 tctaatttcc ttaatgatct caagtatttc ttccttatct accatacatt ttcccgaaaa
     1501 cggtacactt atactttttt caatgagatc ctcaagcgtt tccagaatcg aaagcatttc
     1561 cataataatc tcctcccagt cttattgact tttatcaaca aggactcgcc aatagaattc
     1621 ggtatacttt tatcatactg ctttatcgtt tatcattact ataaatattt tatcaattta
     1681 ttaaagcctt cgcaaaatca aataccaata agcttttgaa cttcaatgct taatttttta
     1741 actgtcttta ttaaattgac aatttactga gcgaacctct ttattaccct gtctcttata
     1801 cactccggaa ccaaaccgtc aattttacct ttatgttctg ccaattccct tacggaactg
     1861 gaactcaaaa acgagtaatg tatgttggtc atcataaata atgtctcaat accggaatca
     1921 agatttttat tcagcaatgc catttgaagc tcatattcaa aatccgacac agccctcaag
     1981 cccttaataa taactttggc attcttattt ctcatataat ctatcaaaag tccggaaaag
     2041 ctttcaactt caacattatg tatgtttttt acactgcact ttataaggtc cactctttcc
     2101 tccagcgaaa acaccggttt cttacttctg tttaccaaaa cggcaacaat aagcttgtca
     2161 cacaatttag aagctctctc aattatgtcc atatgcccgt ttgttatagg atcaaagctt
     2221 cctggatata catatacact cacattcata ccttctcact ttataagttt atctatcgtc
     2281 catacctcta aaaaaggata ttgccgtaat gccatatttc tggcaccggt acctcttata
     2341 accgccaatt tcttcctgaa tcccgtcgtc aaagctatgc tcagcaataa taatcccttc
     2401 aggcttaatt atagcatttt tttctatgca ttttaatgtt tctacaacaa gatttttttt
     2461 atatggcgga tcaagaaata ttatatcaaa ttttttgtta ttttttgata tcttattcaa
     2521 agtaataaaa atatcgcccg taatcacaga agccttgtca attaactttg tattcacaag
     2581 attctccctt attatacggg aacactcatt gcttttatcc acaaatacag caaaatcagc
     2641 ccctctgctc aatgcctcta tgccgatgct gcccgttcct gcataaatat ccaacacccc
     2701 ggcccccggg atcatgcctg agataatatt gaacagggac tctttaaccc tgtcggatgt
     2761 aggtcttgtt gaaattcctt ttaatgtttt aagcttgtgt ccctttgcag ttccggaaat
     2821 tactcttaaa ataacatatt cctccaacta aatacaatat tatatacaat gatattttat
     2881 atcatcatat ataattgtca accataatcc gacttttaat tcctgttaat tattaattaa
     2941 gacttatatc tttgagcttt cctttaaact tttcgactat tttttccttc agcttgatat
     3001 tttcttcctc acacaacagc ctgtcccttc tcataacttc attcgccgct tcctgtgcct
     3061 ttttcaatat atccatatcc ctatataaat ttgcaatttt aagctccggg agtccatgct
     3121 gccttgtccc aaagaaatcc ccaggtcccc ggatctccag atctttttcg gaaataacaa
     3181 aaccatcggt ggagttctcc attactttca ttctctcccg ggcaatctgg ctcttacttt
     3241 cattatacag gatacagtaa gattgatatc cgcctcttcc aacacgcccc ctcagttggt
     3301 gaagctgggc aagaccaaac ctttcagcat tttcaaccac catgacagtt gcattgggaa
     3361 catttatccc cacctcgatt actgtggtgg acaccagaat atcaatttct ccgtccgcaa
     3421 aacgctgcat aaccttgtcc ttttccaatg gcggcatttt tccgtgaaca agccccaccc
     3481 tgaggtcatt aaagtcttct ttggttattt tctctgccat ctccaatgca gatttagctt
     3541 ctatggcatc cgactcctct accagaggac acactatata aacctggtgc ccctccgata
     3601 tgttctttct tataaaactg tttattctgt ctctcttgct gttgtctaca gcataagtct
     3661 ttatgggttt tcttcctgaa ggaagctcat caattaccga tatatccaaa tcgccataca
     3721 atataagggc cagtgtcctg ggaatcgggg tagcagtcat tacaagcaca tcgggattct
     3781 ctccttttcc tgaaagcact gctctttgcc ttactccaaa gcggtgttgt tcgtcggtta
     3841 ttaccagccc taaacggcaa aactttacat tgtcttctaa aagtgcatgg gtaccgatta
     3901 caatatcaac ctctcccgct tcaattcttg aaagcaactc tttcttctgc ttggctgtct
     3961 gactgcccac caaaagagaa atagaaaccc cgcttttctc cattagggaa cataatgtct
     4021 cataatgctg ttttgcaaga atctccgtgg gtaccatcat aatgccctgg tatccgctct
     4081 tgacggcttt caacagtgcc agagcggcaa ttactgtctt tcccgagccc acatctccct
     4141 gaaccaaccg gttcataatc tgggggcttt ccatgtcttc ttcaatctct ctccaaaccc
     4201 ttttttgagc attggtaagt tcaaatcctg tattggctat aaaatgttcc agctcttttt
     4261 catgaggccc aaagcaaatt ccctcttttc catcccccaa tgcactttta atgctgagaa
     4321 gtcccaattg caggataaac agctcttcaa acacaagcct gtatcgtgaa taatttaaat
     4381 cttcttcact tctcggaaaa tgaatattgg aaaacgagta gtttatgtca cacaagcggt
     4441 attcctttct tatccacccc gggagcggat cctcaagcat tccggcaaca atctccaatg
     4501 cgttttctat taccgtccga atggtattct gggataaacc ggccgtcgac ggataaaccg
     4561 gcagaatcct cattgtattt ttatactccc cggtgtcaac cctttcatat accggattgt
     4621 tgacctctaa atatttatac tttccagtta ctttgccgaa aaaaacatag cattctccaa
     4681 cattaaatat ttttttgaca aaaggttgat tgaaccaagt ggctataacg gttccggtat
     4741 catcctttac atatacctga tatatgctaa gcccttttct aatacgtttt tccgatacct
     4801 tcgacatgac aataccctca aaggtacatt cctcaccgtt ttccatctgg gatatttttt
     4861 ttaacttgct tcggtcttca tatcccctcg gaaaaaacgt tataagatcg caaacagaaa
     4921 agactccgag tctgtggaag agcttggacc tcttttctgc cacaccttta atgtactgta
     4981 ttgacttttt cagtatatcc tctataccac tgttcataaa ctttacagtc ctttttcctc
     5041 tttcgtgtgt ttcatgttct taataattat atcatatgag agctgttaaa gcaccaacca
     5101 aaagctccgg agtatttttc tgcacattat tttaagggtg catattattc atgggaagta
     5161 aaataaggaa atacattggt tttacgaatt tttttcttta tatttattta atacaaccga
     5221 attgtaaaaa aaacttgcga ataaaccaga gtatatgtta aaataggtat tgtttgcaaa
     5281 gagctgaatt aaatccgtat ttgattaata tttctccagc tttaaaggag gtgtgattat
     5341 aaatggcaaa gtgtgaaata tgtagtaagg acgtactttt tggcataaag gtcagccact
     5401 cacataggag aaccaataga tcatggaagc cgaatgtaag aaaggttaga attgttgata
     5461 atggaactcc aaaaacagta aatatctgtt caagatgttt gcgttcaaac aaggttacca
     5521 gagcaatata agagtacaaa tttcaaaaca gtgtatcgct gattttttgt gatacactgt
     5581 tttttgtgga atatcacttc gtgaaatccc actaatcagg cacaaatccc aagtgctcaa
     5641 atgctcttgg gatttgtcag tagtatttaa cgtaataaaa tcaagcgtaa agccccaggt
     5701 tttcaagaat attaaagctt tattatattg cgggttttaa tatgttgtat attggtaatt
     5761 aacaggtcag tccattctaa aaacctttct aatgatgttt ccgagaaatt ttggcatctt
     5821 aaataccaat atcttcatgt cattcactcc tcccaggtaa aaaagctctc caataataca
     5881 gtatattcat tttatctgtt ttttgtgaat acttggtttt actcagcttg cctgctttat
     5941 aaccctgctt acagctccaa aaaccacagt ggcaataata gcacctaaaa atgcaggcac
     6001 caatgcaatt tccattgccc ccgggccaaa gacattaaaa tacggcatat acgccccaat
     6061 ccaggcacca atcagtgcaa caagcgaagc tccccatgca tctcccggta ttttatccgg
     6121 actaatggca tttcccagcc accctaccag aactgcaatt aagactgtaa caataaagcc
     6181 gcccatatat agctccctcc tttaacaata taagatttat tattgtatta tttccataaa
     6241 aatgttttat ttacttaacc tcaaaataca atatatagta attgagagct atgcaaaact
     6301 ttcaggggta tttaatttgt ttattatatt cgtaagaaac tataggaaaa aacttttata
     6361 tctttcagtg atacaagaca aagaaaacac ccggtaaaga agataagatg tacagaataa
     6421 gctattttgt tcccgaacac ctgtggggta aaaaagaaaa gaatctattc gagaagatgg
     6481 tatattcctt taaagtgctt tgaattaatt tccacctgta attttcacgc aatttctgcc
     6541 ggattctttt gcatcgtata atgcatcatc cgccatcttc aaaagttccg cttcagagct
     6601 tgaaaactca gggtatgaag acactccaaa actaaccgta accgaagctg tagcctttcc
     6661 atccgtaatt gatgttttgg agataaggtc tctgagcctc tcaaccttat aataggcctc
     6721 attgattcct gttctcggca gaagtatgac aaactcctca cctccatatc tggctataat
     6781 atcatcgctt cttagagaat tttttataag tgttgcaatc cgactcaaaa ccttgtcacc
     6841 aaacagatgt ccgaatgtgt cattaaactt tttaaaatga tcaatatcga gtaatgcaag
     6901 agaaaggtca tacttttcct ttttagcact ttcaaattct tctttaagtc tttcctgaaa
     6961 atagagcctg ttgtaaactc cggtaagacc atccacagtt gccagttcat gcatcctctg
     7021 gtacaactcc acattttcca ttgcaatacc cacctgctgt ccaataatat ttaagagacg
     7081 caggttgttt tcgtcaaaag catttgaata catgtgctca accaatacca aaccaaactt
     7141 tcttgatttt gtaacaaggg gtacgcaaat taaagacttt atgcctctac cctcggtaaa
     7201 gggaaaatca ttggcattca cgcaattttc aataacaggg tctccggttt ccaatacatc
     7261 cataagctta tcgcaattta tattgtcgtt gagtgttata agtccattgc gttttgaaac
     7321 attcgtggta tgaaccttca acctgcattt tttatcatcg tagcgtatta ttgtggaact
     7381 gctgactccc ataactccaa ttattatgtc attgacatct ttcaataaat catcaatatc
     7441 gagtatggaa cttattgcct gggtaatctg ctgcaatgta aacaactcag ccaaactggt
     7501 cattagcttc ttattagtct cctccagctt gcaattggca tccttcaatt tgccgttctg
     7561 caccttaatc tcttcctggg ctgcagctac ctggtcatac ctgtttgcaa gctccataaa
     7621 cagcttcctg ttttgttgtt cattcttatt attgtccgaa taaaaccatg aacaaagaac
     7681 tgccagcaaa aacataacca gatggactga aatgatataa ggaaattctt ttacaatttc
     7741 atctgtaaca gcaaaaccat cttctaaaaa atataaatta ttgacaatcc tcattactat
     7801 atttaacaaa agagagtatc ccaccagaat aaatgaaaac tttcgtccca cacataaggc
     7861 agtaaccatt acaaggaaca agaggatgta atttgcccat tccccggctc cgataaaaac
     7921 catggcaatg tgtataaata gaacctctaa gagctttatg ggcatgtaaa acttattact
     7981 gccaaaaaag tccttccatg caaatacagc tttgatcaaa ctgaatatta tcattcccaa
     8041 gaggaaacag actttaacaa cacccgggca tatgttccag ttcgccaaag taccggcatc
     8101 tacagtaata acatgagcag caatagttcc aataataagc catgtaattc caagtagcat
     8161 cagctcatac tttccaatcc tattcacaga tacacttccc tttcgaacaa agcaagttta
     8221 cttaaaatgt tgtagatgaa atattaaaat attacctcaa tttgtaataa ttcatttcag
     8281 aaattgtctt ccatagtcac aatagtcaat gatgctgcat ttattgcact caggttttct
     8341 tgcatgacac actgctctgc cgtggtatac caattgatgg caaaattccg accaattctc
     8401 tttcggtaca acagccataa ggtcaaattc cacttttttc gggtcattgc tgctgctcag
     8461 acctatccgg ttactcaacc tttttgcatg ggtatcaaca acaatacccg gaataccaaa
     8521 aacgtctccc agcaccaaat tcgcagtctt tcgcccaacc ccgggcaatg tcagcaaatc
     8581 ctccaaattg ccgggaacct gtccattgaa ttggtcaact attattctgc tggcttcctt
     8641 aatatttctt gctttattgc ggtacaagcc tgtgggcatt atatcccgtt ccagttcttc
     8701 caaatctgca ttggcaaaat ccaaggcagt tttatatttc ttaaaaagtg tgttggtcac
     8761 catgtttact ctcacatcgg tacactgtgc agcaagctgt gtagctacaa gcaattgaag
     8821 aggatccttg tagtctagag tacacgtggc atccttatat atctttttga acacctttat
     8881 tatactttct actctttctt tcttatccat acaacagcat accctccaac atattataac
     8941 agataattac ttccattgca tagcaataaa actaaaaatt aataaataaa aaatgatttt
     9001 tgctcaaaaa aagtccagat cgaccggact tttttattta tcatgcacag aataaattgg
     9061 attataaata tacataaatg ctctaatccc atattatttc tttttcatca gttttgcata
     9121 taaatcagag tacttgcccg ctgacttatt ccatgaataa tcggcactca ttgccctctt
     9181 aacgatcata tcccattctt gaggtttttc attatagaac tttatcgccc gcttgagagt
     9241 ctttaacata tccttggacg tgaactccgt gtatgaaaaa ccgtttccat ttttcctatc
     9301 aacatctaca tcaataactg tttccgccaa accgcctgtt gcccgaacta taggaattgt
     9361 accgtatctt aagctaaaaa gctgtcctaa tccacagggc tcaaaccttg acggcataag
     9421 gaacatatca cagcctgcat atatcctctg ggctaaagga acattaaaac ctatatatac
     9481 tccaaccttt tcagggtact tttcctgaat cttcttaaat cctgtctcat aatactcatc
     9541 acccgcacca agcaagataa actgtatgtc atctttcatc atttcatcta tttcatccat
     9601 tataaggttt aagcccttct gccccgtaag tctcgatatc aaaccgatag ccggcacatc
     9661 ctttttggga agtcccattt ccttctggag tgcatacttg ttttcaactt ttagcttgaa
     9721 ggtctcaaca ttatagtttt tattaattcg tgggtcggtt tcgggattga aaacatcata
     9781 gtctatgccg ttcactattc catacagatc ctttgacctg ttttttaata gcccctccaa
     9841 tttttcgcca tatctttcag tttgaatttc ttttgcatag gtttcactta ccgtacttat
     9901 tatgtcggca taaaccaaac ccgctttcat aaaactgaac ataccgtaaa actcaacctt
     9961 ttcaggtaca aaaagctcat cgggtattcc cataataccc agtacatcct tcgagaaatt
    10021 gccctgatat tcaaggttgt gaaccgtgaa caaggttttt attttcttgt aataaggata
    10081 agatttataa ttttccttca ggaacataca aatcggtcct gtatgccaat cattacagtg
    10141 aattatatcg ggcttgaaat caattttagg aagcatctca agaaccgctt tgcaaaaaaa
    10201 ggcgaacctg tcggcgtcat caaaataaca atatataccg cctctattaa aatagtggta
    10261 attatctaca aagtacacag gcaccttgga atccttttcc ttatccctaa agtctatgga
    10321 accttccctg acaatgcagg tagcctgtct gttgcccatt tctacaggaa aatccgtcac
    10381 ataactcata tcagcgttta tttgctgata cctaggcatt gcaatcctga catcatatcc
    10441 catggaaacc agtgatttgg gcaatgaacc cgctacatct gccagtcctc ctgtttttgc
    10501 gaatggagct acttcaacag aaacaaataa caccttcaag ctatcatttc ctctcatttt
    10561 atacctccat cttgtttttt tacagggctt cgagcccctt cgctttttgc tatttcactc
    10621 cgtgaaatag cactcttt
//
LOCUS       DAOOJC010000022        10343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_5794_length_10343_cov_3.913005, whole genome shotgun sequence.
ACCESSION   DAOOJC010000022 DAOOJC010000000
VERSION     DAOOJC010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10343
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5794_length_10343_cov_3.913005"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..586)
                     /locus_tag="PLA01_01190"
     CDS             complement(122..586)
                     /locus_tag="PLA01_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HPC99945.1"
                     /translation="MNVEYINPFIEASQSVLKQIAKIDARLGEVYIKTSPYTGDNILI
                     FVGLTGKIRGQAIFAMSKKVALIIASNMMGGAEIIELDEISKSALSELTNMILGNTAT
                     ILYNRGIGIEITPPSFLMGDNMQISPTKMKTICIPLRFEDDKQIEIHISVIE"
     gene            complement(889..1452)
                     /locus_tag="PLA01_01195"
     CDS             complement(889..1452)
                     /locus_tag="PLA01_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HPC99946.1"
                     /translation="MYKKRVIFVTDGDMVAMRAVEIATANIGGRCISASAGNPTTLTA
                     EEIVEMIKKAEHDPVVVMVDDRGYKGKGKGETAMEAVMNNDYIETLGVLAVSSKGKDC
                     GKFKVSCSVTREGKIIEGTVDKDGKETHTGLVCGDTLSILREHDDMVIVGIGDPGKMD
                     FNDEIEKGAPITTKALNEVLKRSAARH"
     gene            complement(1508..1864)
                     /gene="spoVAE"
                     /locus_tag="PLA01_01200"
     CDS             complement(1508..1864)
                     /gene="spoVAE"
                     /locus_tag="PLA01_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HPC99947.1"
                     /translation="MSYVNAFIVGGVICAAGQILIDKTKLTSARILVLFVTAGAILTA
                     MGIYQKIVDIGGAGATIPLTGFGYNLVKGTFSDVDKIGVLGAFTGGIKATAAGIAAAV
                     FFGYFMSVAFTPKAKR"
     gene            complement(1913..2932)
                     /gene="spoVAD"
                     /locus_tag="PLA01_01205"
     CDS             complement(1913..2932)
                     /gene="spoVAD"
                     /locus_tag="PLA01_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HPC99948.1"
                     /translation="MSTKRLGNQTVKLQNPPSIVSTACIAGPKEGKGPLRLYFDNIVE
                     DEMCGEKSWEKAESKLIRETFALILKKANKSAEQINYVFSGDLLNQCIAANFGLRESE
                     VPFFGVYGACSTMAESMSLGSMLIDGGFAEDIVCVTSSHFCSAEKQFRFPLELGTQRT
                     PTAQWTVTGSGGVLLSAKGDGPYITHITTGKIVDKGIKDANNMGAAMAPAAVDTIVTH
                     FMDTGFSPERYDLIVTGDLGEVGRDICAELVQEQGYNISGIYNDCGLMIYDRERQDTH
                     AGGSGCGCCATVFAGFIYWKMMEGSLKNVLFVATGALLSPTSTQQGESIPSIAHAVSI
                     STVLQ"
     gene            complement(2948..3391)
                     /gene="spoVAC"
                     /locus_tag="PLA01_01210"
     CDS             complement(2948..3391)
                     /gene="spoVAC"
                     /locus_tag="PLA01_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HPC99949.1"
                     /translation="MKKTMTNKEYQIYVEKKSPKSNIGRNIIRAFIVGGLICIIGQFF
                     TNALKMRGYGQEEVAGITSLLMIFLGALFTGLNLYDDLGRFAGAGSIVPITGFANSIV
                     SPAMEFKSEGYVMGVGGRMFVIAGPVLVYGISASIIAGIVYYLLS"
     gene            complement(3409..3594)
                     /locus_tag="PLA01_01215"
     CDS             complement(3409..3594)
                     /locus_tag="PLA01_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99950.1"
                     /translation="MAEVLRRHKFVLIVLSLLIIITFLAFTLLMRKQEKIPSKGIFVL
                     QTCDEISLFEFSGKRIA"
     gene            complement(3693..4433)
                     /gene="sigF"
                     /locus_tag="PLA01_01220"
     CDS             complement(3693..4433)
                     /gene="sigF"
                     /locus_tag="PLA01_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HPC99951.1"
                     /translation="MDEIFEKETLELIREAKKGNKDAQSVLVEKNIGLVWSIVRRFQN
                     RGYETEDIFQIGCIGLIKAISKFDCSFDVKFSTYAVPMIIGEIKRFIRDDGIIKVSRS
                     LKELSNKARVTKEIMTKELGREPSVSEISSRLEIPVEDLVMAMEASCTPESLYSTVGE
                     GDNSSALLIDRINNESNDNEIDLVDKIDLKKVLDTLKPREKQIIILRYFKEKTQVQIA
                     KMLGISQVQVSRIEKKILEDIRKKIKYV"
     gene            complement(4582..5016)
                     /gene="spoIIAB"
                     /locus_tag="PLA01_01225"
     CDS             complement(4582..5016)
                     /gene="spoIIAB"
                     /locus_tag="PLA01_01225"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HPC99952.1"
                     /translation="MNPINEVKLEFISKSSNEAFARVVAAAFVSQIDPNLEELADVKT
                     AVSEAVTNAIIHGYENSSGIVKMECKLYTNAVEITVIDEGKGIEDIEQARQPMYTSRP
                     EMERSGMGFTVMENFMDKVEVVSEAGKGTTVKMYKVFKSVGN"
     gene            complement(5030..5365)
                     /gene="spoIIAA"
                     /locus_tag="PLA01_01230"
     CDS             complement(5030..5365)
                     /gene="spoIIAA"
                     /locus_tag="PLA01_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HPC99953.1"
                     /translation="MNLKLTNRGTTLIAAITGELDHHSAEHIIQKLDAEIMKSTTKNL
                     IMDFSKVNFMDSSGIGVVIGRYKKIQRLNGKLFITGINDKISRIFNISGLLKLVPACE
                     NIDDAINKM"
     gene            complement(5631..5900)
                     /locus_tag="PLA01_01235"
     CDS             complement(5631..5900)
                     /locus_tag="PLA01_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPC99954.1"
                     /translation="MVEQTIKITNPSGLHARPAAMFVQTAGKFTSNIWIKKGERQVDA
                     KSIMGLMSLAVAQGEEIEISADGEDEKLAVNELVDLVTSSFGEYD"
     gene            complement(5906..6850)
                     /gene="whiA"
                     /locus_tag="PLA01_01240"
     CDS             complement(5906..6850)
                     /gene="whiA"
                     /locus_tag="PLA01_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HPC99955.1"
                     /translation="MSFSSTVKNELCRINIGSNSIFICELAAAIRISGIIKVVSPDEI
                     NMRIITENAAFARRIFSLVKELYGINVEMSIRRSRKLKKHIVYMLVLTCSKGLKKVLS
                     DANIKTSDTVEYLPYAGTINRKSGLKAYLRGAFLASGSMSDPEKTYHLEIICHNHALA
                     KELNEIMDSFNLNAKVIMRKGSYVVYLKEGENIVDFLNIIGAHSALLELENIRILKEM
                     RNNVNRIVNCETANLQKTVNASVRQVDNIRYIRDNLGFEKLPENLRDIAQLRLKYSDA
                     SLKELGEMLSPSLGKSGVNHRLRKLDIIAERIRSMKGE"
     gene            complement(7009..8973)
                     /locus_tag="PLA01_01245"
     CDS             complement(7009..8973)
                     /locus_tag="PLA01_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HPC99956.1"
                     /translation="MDFGKSSWRTYEQGIQKEWLLTNGIGGFSSSTIIGSNTRRYHGL
                     LVASLKPPVARHLILSKIDESVTVDDEVYNLFSYEVPGFIQKGYHYLERFQYNFIPKY
                     VYRVKDVYIEKEICMVYGENTVAVVYRIINGTKEVNLRLTPLVNFRDYHFNSSRAYMN
                     FVKEYKSKILTVRPTYYDIDISLYCSEGKYKDLDDTWFYNMDYAVERERGLSSTEDHY
                     IPGCFDIRIEPMEQKCITFICTVEKNIGCKDGIKIINSEEKRLVSLIDKAGYKDELAK
                     RFVLAADNFIVHRKSTNAKTIIAGYPWFTDWGRDTMIAFTGLTLSTRRFEDAKEILYT
                     FSKYIKDGLIPNMFPDGGNEPPYNSVDAPLWYFVATKKYADYTKDYDFIEKNLYSVLK
                     EIIESYTKGTRFDIKMDEDYLITAGDEHTQLTWMDAKVGDWVVTPRHGKTVEINALWY
                     NALKVMSELSKHLKQEFAGYDELAQKVKESFVKSFWNEEKQCLYDCITKDYTDDKVRP
                     NQIMAVSLDYPVIEGERAEKVVNKVFSELYTAYGLRSLSSESGEFVGVYAGDQYRRDG
                     AYHQGTVWTWPLGQFITAYMKVNNYSSWAKEVAVKFIEPFIDHFEDACLGSISEIFDG
                     NEPLIPRGCFAQAWSVAEILRAYVEDVMRL"
     gene            complement(9066..10223)
                     /locus_tag="PLA01_01250"
     CDS             complement(9066..10223)
                     /locus_tag="PLA01_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HPC99957.1"
                     /translation="MRIINVVKQIFTNGTVEKDGKDTLKTSFLQEEVETLTCEEKRME
                     SDPKIVTIGGGTGLSTMLRGLKVYSSNITAVVTVADDGGGSGVLRQDLGILPPGDIRN
                     CILALANTEPIMEKLLQYRFQDGMLKGQSFGNLFLAAMDGICPSFEQAVQRMSDVLAV
                     KGRVLPVTLQDIKLCAQLEDGYVIVGESKIGEHHKFHKGSIKKVYLEPEFAEPLDEVI
                     EAINEADVIVLGPGSLYTSIIPNLLVNGVCEAIRDSKALKIYVCNVMTQPGETEGYTV
                     EDHIGAIERHSFKGIVDYCIYNTADIPEDLKRKYRDDGAEVVRLETGEMRISGIKLLG
                     EDFVCINNSYIRHDTKRLAQTIINLVEKTLFSRGKKMPTYYNKNRFKDMAQ"
     gene            complement(10220..>10343)
                     /locus_tag="PLA01_01255"
     CDS             complement(10220..>10343)
                     /locus_tag="PLA01_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPC99958.1"
                     /translation="GGRHRSVAISDELFSYLSEREHRVVIDHRDIDKDGRGAKK"
BASE COUNT         2927 a         2465 c         1504 g         3447 t
ORIGIN      
        1 aacaaaaaac ggcatagccg aatatcaacg actatgccga atttttcagg aattaataat
       61 ccctatctga ccgctcctaa tggccttttt ttatttctat aataaaaact ataatataaa
      121 actattcgat tacggatata tgtatttcaa tttgtttatc atcttcaaaa cggagtggaa
      181 tacatatcgt cttcattttt gtcggcgata tctgcatatt gtcacccatg agaaatgagg
      241 gtggtgttat ttcgatacct attcccctat tgtaaagaat agtggcagta tttccaagaa
      301 tcatatttgt aagctccgaa agagcacttt tagatatttc gtccagctct ataatttccg
      361 cccctcccat catattggaa gctattatca aagcaacttt cttgctcatt gcaaaaatcg
      421 cctgtcctct tatttttccg gttaatccta caaatatcag aatattatcc cctgtataag
      481 gagacgtctt aatgtatact tctcccaatc gggcatcgat ttttgcaatc tgctttaata
      541 ccgattgact agcttctatg aaagggttaa tgtattcgac attcataaat caataccacc
      601 tttttttata tgtgctgcta aaaaactaag acgcattagt gatgcttggt acaataccag
      661 acactatatt ttaataagat ttcacaatga tagccaatgg taattgaaat tcagttcagg
      721 tgaatacaaa cagcaatgag gtaaaaaaga ataattttcg tattatttat tattctataa
      781 acctgttata aattcctctt ttttttctac actttttcgc aagtatttct atgctgtact
      841 cttttgtatt tttcagcatc cacatattac ctagtgtgct gcaagacatc aatgccttgc
      901 agcacttctt ttcaaaactt catttaaagc tttagtagta atgggagcac ctttttctat
      961 ttcatcatta aaatccattt tccccggatc ccctattcca acaatgacca tgtcgtcatg
     1021 ttccctcaat atactcaatg tatcaccgca aactaatccg gtatgagttt cctttccgtc
     1081 tttatccaca gtaccttcta ttattttacc ttctcttgtt accgaacatg ataccttaaa
     1141 cttgccgcaa tcctttcctt tcgaagatac ggctaaaacg ccaagggttt caatataatc
     1201 attgttcata actgcttcca ttgctgtctc acccttacct tttcctttat atcccctgtc
     1261 atctaccatc accaccacag gatcatgttc ggctttttta atcatctcta caatttcttc
     1321 agcagtcaat gtagttggat tgcccgcaga agcagaaata catctgccac cgatattagc
     1381 cgtggcgatt tccacggctc tcatagctac catatcacca tcggtgacaa aaatcactct
     1441 ttttttatac attttatcac acctatattt taaattttta agccatatgc cggacaaaaa
     1501 aatattgcta cctttttgct tttggtgtaa atgcaacaga cataaaatat ccaaaaaaaa
     1561 cagctgcggc aattcctgct gcagtagcct ttattccgcc ggtaaacgca ccgagaacac
     1621 ctattttatc tacatcgcta aaggttccct ttacaaggtt gtaaccaaaa cctgtaagag
     1681 gaatggtcgc tccggcacct ccaatatcca caatcttctg ataaatcccc atggccgtca
     1741 atattgcacc tgctgttaca aacaatacaa gtattctcgc agatgtaagc ttcgttttgt
     1801 caattagtat ctgccccgcc gcacagatta ctcctccaac aataaaggcg ttaacataag
     1861 acattaaaac attatccata aaatcacccc aaaaatgagt attggcattt tactactgta
     1921 ataccgtaga aatacttact gcatgtgcta tagatggtat cgattcccct tgttgggtgc
     1981 ttgtggggct taagagagcc ccggttgcaa caaataatac atttttcaga cttccctcca
     2041 tcattttcca ataaataaat cccgcaaaca ctgttgcaca acaaccgcat ccactgcctc
     2101 cggcatgtgt atcctgcctt tcccggtcgt atatcatcaa accgcaatca ttatatatgc
     2161 cgcttatgtt atagccctgc tcctgcacaa gctcggcaca tatatctctt cccacctcac
     2221 caaggtctcc tgtcactatc aggtcatatc tttcgggaga aaaacctgtg tccatgaagt
     2281 gggtaacgat agtatcaacc gcagccggtg ccatggctgc tcccatattg ttggcatcct
     2341 taatgccttt atctacaatt ttgccggtag tgatatgggt tatatagggt ccgtcacctt
     2401 ttgccgacaa caaaacacct ccggatcctg tcactgtcca ttgtgctgtt ggagtacgct
     2461 gcgttccgag ctccagagga aacctgaact gtttttccgc tgaacaaaaa tgacttgacg
     2521 taacacacac aatatcttcc gcaaatccgc cgtctataag cattgaacct aaactcatcg
     2581 actcagccat agtcgaacaa gcaccataaa ctccaaagaa aggaacttcc gactctctaa
     2641 gtccgaaatt tgcagctata cattggttta aaaggtctcc tgaaaaaaca taatttattt
     2701 gctcggcaga tttattggct ttctttaaaa tcagtgcaaa ggtttctctg attaatttgc
     2761 tttcagcttt ttcccagctt ttctcgccgc acatctcatc ttcaactata ttgtcgaaat
     2821 acagccttaa aggccctttt ccttctttcg gtcctgcaat gcaagcagtt gaaacaatac
     2881 ttggaggatt ctgaagcttt actgtttgat ttcccaatct ttttgtactc atattccact
     2941 ccttgcccta gcttaataga taatatacaa ttcctgcaat tattgatgca gaaattccat
     3001 atacaaggac ggggccggca ataacaaaca ttcttcctcc cacgcccatc acatatcctt
     3061 cactcttaaa ctccattgct ggtgaaacga tagagtttgc aaatcctgtt ataggaacta
     3121 tagaacctgc accggcaaat cttcccagat cgtcatacag attaagtcct gtaaacaatg
     3181 cccccaaaaa tatcatcaaa agtgatgtaa ttccagctac ttcttcctgt ccatatcctc
     3241 tcatttttaa tgcattggta aaaaactgtc ctattataca aataagtccc ccaactatga
     3301 aagccctgat tatatttctg ccgatattgg atttcgggga ttttttctcc acataaattt
     3361 gatattcttt attggtcatt gtttttttca taattaacct cccggctttt aagcaattct
     3421 ttttcctgaa aactcaaaaa gagaaatttc atcacaggtc tgtaaaacga aaattccttt
     3481 tgacggaatt ttttcctgct ttcgcattaa taacgtgaat gctaaaaagg taatgattat
     3541 taaaaggctt aaaactatta acacaaattt atgccttctt aatacttctg ccatattgaa
     3601 tcaatccttc atacttataa tttctatata gtttagtatt gtaaaaaaaa aaaacaatat
     3661 gcaggaacat attgttttat tatatatgca ctctatacat acttaatttt ctttctaata
     3721 tcctccaata ttttcttttc gattctcgaa acctgaacct gtgaaatacc cagcattttt
     3781 gctatctgaa cctgggtttt ttccttgaaa tatctcaaaa taataatttg cttttccctt
     3841 ggtttaaggg tatcgagtac ctttttaagg tcaattttgt caacgaggtc tatctcattg
     3901 tcattgcttt cattattaat cctgtctatc aaaagtgctg aagaattatc cccttctcca
     3961 actgtgctat ataacgattc cggtgtacag cttgcttcca ttgccataac caaatcttca
     4021 actggaattt caaggcggga tgaaatctcg cttaccgacg gctcccgtcc caattccttt
     4081 gtcataattt ccttggtaac tctggcttta ttggataatt ccttgagaga acggctaacc
     4141 tttataatac cgtcatccct tataaatctc ttaatttctc caatgatcat cggaaccgca
     4201 tatgtagaaa atttgacgtc aaaggaacaa tcaaacttgc ttatggcctt tataagtcct
     4261 atacaaccta tctggaaaat atcctcagtt tcgtatcccc tgttctgaaa tcttctcaca
     4321 atactccaaa ccaggcctat atttttttcg accaatacgg actgagcatc cttgtttccc
     4381 tttttagcct ctcgaattaa ttccagagtc tccttttcaa atatttcatc catatttata
     4441 tacattcctt tcgcaagctc aaggcacaaa acaccaaaaa tattattaaa aacctactcc
     4501 ctcaatatct caaatcgtgt aacaactgat aaccgctaga attattctta acattcaaca
     4561 agtttttaaa cattacccgt gttaatttcc aacagacttg aagaccttat acatttttac
     4621 tgttgtccct tttcctgctt cagaaacaac ctcaaccttg tccataaaat tctccatgac
     4681 agtaaatccc ataccggatc tctccatctc cggtctggaa gtatacatgg gttgccttgc
     4741 ctgttcaata tcttctattc ccttaccttc atcgattacg gttatttcta cggcatttgt
     4801 atatagtttg cattccattt ttacaattcc gctgctgttt tcataaccat gaattatggc
     4861 attggttaca gcttccgaaa ctgcagtttt tacatctgcg agttcttcca gattcggatc
     4921 tatttgtgat acaaaagccg ccgcaactac tcttgcaaat gcttcattgc ttgacttgct
     4981 gataaattcc aattttactt catttatagg attcattcta tattcccctt tacattttat
     5041 ttatagcatc gtctatgttt tcacaagctg gtaccaattt taaaagtccg gatatattaa
     5101 aaatccgact gattttatcg ttaatacccg ttataaataa tttcccgttt aacctttgga
     5161 tttttttata tcttccaata acaaccccaa tacctgaact atccataaaa ttaacctttg
     5221 aaaagtccat aatcaaattc tttgtagtgg atttcattat ttctgcatcc agcttctgta
     5281 taatgtgttc ggcagaatga tgatccagct ctcctgtaat ggcagcaatt aaagttgtgc
     5341 ctctgtttgt taattttaaa ttcaatatta tcctcctcca acctttgtca tcctaaactt
     5401 tattcttcat agactatatt tccccttcat agaataatgt aaggttttgt aatttgggca
     5461 agaaaatatc gacaaataac aacaagaaaa aataccttta atttgattaa aaggtatccc
     5521 aatacttata aggcattata taacattgat aaatatatag acctatcagt gccacacaat
     5581 ggcactaagt gcttcatgag caaagcatgc tcgtcataat ttattccagg ttaatcatac
     5641 tctccaaagc ttgacgttac aagatcaaca agctcattca ctgccaattt ctcatcttca
     5701 ccgtctgcac tgatttctat ctcttctccc tgggcaacag ctaaggacat aagacccatt
     5761 atgctctttg catcaacctg tctctcaccc tttttaatcc atatgttaga tgtaaacttt
     5821 ccggctgttt gaacaaacat tgcggccggt cgtgcatgca accctgatgg attagttatt
     5881 ttaatagtct gttcaaccaa tttgattatt cccctttcat acttcttatt ctctcagcaa
     5941 taatgtccag tttcctcaat ctatgattaa ctcctgactt tcccagcgaa gggcttaaca
     6001 tttctccaag ctccttaagg ctggcatcgc tgtatttaag ccttagctgt gcaatatctc
     6061 ttaaattctc aggtaatttt tcaaagccaa gattatcccg gatatacctg atgttgtcca
     6121 cctgcctaac cgaagcattc accgtctttt gaagatttgc tgtctcacaa tttactattc
     6181 tgttgacgtt attgcgcatc tcctttagaa ttcttatatt ttcaagttct aagagtgcag
     6241 aatgtgctcc gattatgttt aagaaatcaa ctatattttc accctctttc agatatacca
     6301 catagcttcc ttttctcata atcacttttg catttaaatt gaaactgtcc attatttcat
     6361 tcagttcctt tgccaatgcg tggttatgac atattatctc aagatgatat gtcttttcgg
     6421 gatcactcat cgaacctgaa gctaaaaaag ctccccttaa atatgccttt aggccgctct
     6481 tcctgttgat cgtccctgca tacggcaaat attccactgt gtccgatgtt ttaatattag
     6541 catcactaag tactttcttc aaaccctttg aacatgttag caccagcata tatacaatat
     6601 gttttttaag cttccggctt cttcttatac tcatttccac atttataccg tacaactctt
     6661 taacaagtga aaaaatcctc cgggcaaaag cagcattttc agtaataatc ctcatgttta
     6721 tttcgtcagg gctcacaact tttattattc cgctgattct gattgctgcc gcaagctcac
     6781 aaataaaaat gctgttgctg cctatgttta ttctgcataa ctcattctta actgtagatg
     6841 aaaatgacaa ctcatacacc tcatataata aataatcctt caacttatga taatttatcc
     6901 ggcagttaat ccgtacacaa attataccct aaatctcaat aaatttctag tgccccatga
     6961 cagaaaaaca gaaaccgatt cctgtacatg ccggtttgaa tcatctactt ataaccgcat
     7021 tacatcctca acatatgccc tcaatatttc agccacactc catgcctggg caaagcagcc
     7081 tctgggtatc agcggttcgt tcccgtcaaa tatttcggat atggaaccaa ggcatgcatc
     7141 ctcaaaatgg tctataaacg gttcaataaa ttttaccgcc acttccttag cccaagatga
     7201 ataattgttc accttcatgt atgcagttat aaactgacca agaggccacg tccagactgt
     7261 tccctgatga tacgcaccgt ctcttctgta ctggtctccg gcatacactc ccacaaactc
     7321 accggattcg gaagataaac tcctcagtcc ataagcagta tacagctcac tgaaaacctt
     7381 gtttacaacc ttctcagccc tttctccttc aatcacggga taatccaggc ttaccgccat
     7441 tatctggtta ggacggactt tatcatctgt gtaatccttt gttatgcagt cataaaggca
     7501 ctgcttctct tcgttccaga agctcttaac aaacgattcc ttaacctttt gtgccaattc
     7561 atcgtagccg gcaaattcct gcttcaaatg ctttgacagc tctgacatca cctttagtgc
     7621 attgtaccaa agggcattaa tttctaccgt ctttccatgc ctcggtgtaa ctacccagtc
     7681 acctaccttt gcatccatcc atgtaagctg tgtatgttcg tctccggcag taataaggta
     7741 atcctcatcc atttttatgt caaaccgcgt tccctttgta taggactcga ttatctcctt
     7801 tagcacgcta taaaggtttt tttcaataaa gtcatagtct ttggtataat cggcatattt
     7861 ttttgtagcc acaaaatacc ataaaggtgc atccacactg ttgtatggcg gttcatttcc
     7921 accatcgggg aacatgtttg ggataagccc atccttaatg tattttgaaa aggtatagag
     7981 tatttccttt gcatcttcga accgtcttgt tgagagggta agcccggtaa aagctatcat
     8041 ggtgtccctt ccccaatcgg taaaccacgg gtatcccgca attatggttt ttgcattcgt
     8101 tgatttgcgg tgaacgataa aattgtccgc agccaaaaca aatcttttag ccagttcatc
     8161 cttatatcct gctttatcaa tcaatgacac aagccttttt tcttcgctat tgattatttt
     8221 tatgccatcc ttgcagccaa tgtttttttc gactgtgcag ataaacgtaa tgcacttttg
     8281 ctccatcggt tcaattctta tatcaaaaca accgggtata tagtggtctt cggttgagga
     8341 aagacccctc tccctttcta ccgcataatc catattataa aaccatgtgt cgtccaaatc
     8401 cttatatttt ccctcactgc agtacaaaga aatgtcaatg tcgtaatagg tgggccgaac
     8461 agttaatatc ttgcttttgt attcctttac aaaattcata tatgccctgc tggaattaaa
     8521 atgatagtcc ctgaagttta ctaaaggggt aagtctcaag ttcacttctt ttgttccgtt
     8581 tatgattcgg taaaccacgg caacggtgtt ttctccataa accatgcata tttctttttc
     8641 aatataaaca tccttcactc tgtaaacgta cttcggaata aaattatact ggaagcgttc
     8701 caggtaatgg taccctttct gtataaagcc cggtacttca taggaaaaaa gattataaac
     8761 ttcatcatca acggtaacac tctcgtcaat ctttgataaa atgagatgtc tggccacggg
     8821 aggtttgagc gatgctacca aaagcccgtg atatcttctt gtatttgaac cgattatggt
     8881 tgaggatgaa aacccgccaa ttccgttagt caaaagccat tccttctgta ttccctgttc
     8941 ataagttctc cagcttgatt ttccaaaatc catgcttccc atcctccata tctgattttt
     9001 atccaagcca aaactctacc ttgcataagg cagagctgct cttaataata tataaaaatt
     9061 acaaattact gggccatgtc tttaaaccga ttcttattgt aataggtcgg catttttttg
     9121 cctctactga acagagtttt ttcaactaaa ttaataattg tctgtgccaa cctttttgta
     9181 tcatgtctaa tataagaatt atttatacaa acaaaatctt cacccagcaa tttaatacct
     9241 gatattctca tctccccggt ttcaagtctt actacctctg ctccgtcgtc tctatacttt
     9301 ctcttcaaat cctccggtat atcggcagtg ttgtatatgc agtaatcaac aattccttta
     9361 aaggaatgcc tttctattgc cccgatatga tcctccacag tatacccttc cgtctctccc
     9421 ggttgggtca ttacattgca cacatatatt ttcaaagcct ttgaatctct tatcgcctca
     9481 cacactccat taaccaaaag atttggaatt atgcttgtat aaaggcttcc aggacccaat
     9541 acaataacat cggcctcatt tatcgcctct attacttcat ccagaggctc tgcaaactcg
     9601 ggctccaagt acaccttctt aatggacccc ttatgaaact tgtgatgttc acctattttt
     9661 gactcgccaa caatcacata tccatcctct aattgtgcac aaagcttaat atcctgtaaa
     9721 gtaaccggaa gtacccttcc ctttactgcc agcacatcac tcattctttg aactgcctgc
     9781 tcaaagctgg gacaaatacc atccattgca gcaagaaaaa gattaccgaa gctttgacct
     9841 tttaacatcc catcttgaaa cctgtactgt aacagttttt ccatgatggg ctccgtattg
     9901 gccagtgcca aaatacagtt tctgatatct cccggaggta aaattccaag atcctgtctt
     9961 aaaactcctg atcctcctcc atcgtcagca accgtgacaa cggcagtgat atttgaactg
    10021 taaaccttaa gacccctcaa catggttgaa aggccggtac ctcctccaat tgtcacaatt
    10081 tttggatcac tttccattcg tttttcttca caagttaagg tttcaacctc ttcttgcaga
    10141 aaagatgttt ttaaagtgtc cttcccgtct ttttcaactg tcccgtttgt aaaaatttgc
    10201 tttaccacat taataatcct cattttttgg cacccctgcc gtctttatct atgtccctgt
    10261 gatctataac aactctgtgc tccctctcgg aaagatacga aaacaattca tccgaaatag
    10321 caaccgatct atgtcttcct ccg
//
LOCUS       DAOOJC010000023        10154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_5904_length_10154_cov_3.896227, whole genome shotgun sequence.
ACCESSION   DAOOJC010000023 DAOOJC010000000
VERSION     DAOOJC010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10154
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5904_length_10154_cov_3.896227"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLA01_01260"
     CDS             complement(<1..260)
                     /locus_tag="PLA01_01260"
                     /inference="COORDINATES: protein motif:HMM:NF015783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HPC99959.1"
                     /translation="MSVEKGKISSQQLLFLIAGFVQGSVLLTDFTAGITEHQTWMVIL
                     AGLAVSTPFFLSYVMLAKRFMGMNLVQINDMVYGRFLGKIIS"
     gene            complement(262..1443)
                     /locus_tag="PLA01_01265"
     CDS             complement(262..1443)
                     /locus_tag="PLA01_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HPC99960.1"
                     /translation="MRKKMNLPIMKLILCFFMLFTLSGCFGGREINDLEIVVGIGVDK
                     DENTGNILLTAQIVREEENGNTEKNGGGENGAFWNVTSTGNSIFDAVRQITHKTGNRL
                     FISHNQAVIYGEDIASEGLEKYIDFFLRAHEMRPTALILVAEDRASDVLETKPKTEKF
                     PAANIEKLVESYVFTSQLYKVNMKDFASRLMSTTTAPVAPLVSVLQDNGNKDIYVSGL
                     AVFNKGKMVGKLNQAEVRGLLWVLGKVKSGVIQVCSPNEQGSAVLEILEAESKVTPKL
                     RDGKIVMHIKIKEESSLSEQTTTENLATDPAFEKMQKASEEIIRQEILAAFDKSKELN
                     ADIFGFGEMLHKKYSNEWKALKDNWDDLYPSVELKIDVEAKIIKTDLLKKPAWQSEGG
                     K"
     gene            complement(1500..3077)
                     /locus_tag="PLA01_01270"
     CDS             complement(1500..3077)
                     /locus_tag="PLA01_01270"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HPC99961.1"
                     /translation="MFRYIYKKIRCSLLKNIKKRMHQDTLPGKNNSERIFPDLKRNLE
                     VIKSILHNSSDLTIHEFNFGHDRRFKGAVIFINSMADKGEIDKNILQPLMYGTLMLKK
                     DADMDFASIDSVRKNLIPITDSLKVTLLNDLLDNLLAGSTILLIDRSKEALVINSGQR
                     RSRAIDEPATEVVVRGPREGFNENVNVSIALIRRKIKVPDLCVEKMIIGDKSKTDVYI
                     VYIKSVANPKLIEEVSRRLNRIKIDAILESGYIEQFIEDSSYSIFPTVSNSEKPDKVA
                     AKILEGRVAILVDGTPFVLIVPMLFIESFQSSEDYYSRPFHASIMRIVRFLSYIISIL
                     GPAIYVALTVFHQEMIPTQLLISIAEGRERVPFPAILEAFLMMFTFDILREAGVRLPK
                     SVGQTIGIVGAIVLGQASVEAGLISPIMVIVVSATAIASFTVPAQTDSGTILRYIYLA
                     LAGLAGGFGVIMGLLVTLIHLASLRSFGVPYFWPVVPFDFSGLRDVFIRMPLRKLKKR
                     PKAIVYGGFDSYNHNPG"
     gene            complement(3225..3737)
                     /locus_tag="PLA01_01275"
     CDS             complement(3225..3737)
                     /locus_tag="PLA01_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HPC99962.1"
                     /translation="MLNQEIVKLLNEQVNKEFYSAYLYLDMANYYADQNLSGFENWFY
                     VQAQEERDHAMLFRQYLLNNGESVILTEIAAPNEKYNNYKDPLVKAYEHEKYVTASIN
                     NIYEAAMKIKDFRTMQFLDWFISEQGEEEKNADDNIKKYDLFAGDPKGLYMLDNEMKA
                     RVYTAPSLVL"
     gene            complement(4059..5681)
                     /locus_tag="PLA01_01280"
     CDS             complement(4059..5681)
                     /locus_tag="PLA01_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99963.1"
                     /translation="MNKILKPPALSTSFSENGKRAKKRFDNILNIKAKKTGALAFVSM
                     LIFVGVVGMLVGFNSGKNITYFCNTFDCCFELPRSWENKYEIEERNANIVYVYHKAIR
                     KEYGEGTGLLFYIEMLKGDKLTHEDITDPGNRSIALQGDGYTYVFGMPTDVQYPIWDG
                     GNKELADDYLKMSEDNEKIKKSIRRVTANITGTGTAIVTEDTDLLADPQGNGVGVELM
                     KNDLVYVIYDENDDFYYVQLPVMSIPPTEGYISKKAVSFDKNLFDNANFGIVKNGKIY
                     KSTNEKDIYQEGHKNVFRIIKRQGEWAEVNLVGGDDGKWVKISDISYELIPYNIDLSY
                     TDMQKEVDNGRQLWRLNPLEVAQDYVYNTLKKSGGTFEITHSDDDKSMVTYTSDENED
                     SVIHFHRPIKKDHGGIWVVDDNTIMMDKIRGVIPKGKQIAVNSGMGWNIRVTDKTDYK
                     NNDYGDDFNLEQEAKKLGINIYDYIGHDLKVLIYGIETAEDSSREYTYSPYIFVFEGT
                     NMIAYKDLKSMENEMIARDIMITLYEHNNAGY"
     gene            complement(5674..6528)
                     /locus_tag="PLA01_01285"
     CDS             complement(5674..6528)
                     /locus_tag="PLA01_01285"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HPC99964.1"
                     /translation="MLKNIFAISLSMSAVILLLLIVSPLLNKRYSAKWRYFIWLIIAI
                     RLIIPVRIELPEAPVNISVPNQTVVFRQEGVPIMVTDDSYTHKANTSPVSADYAPVIT
                     LQELLAVVWGTGAVGFFLYHIINYIIFKKKIKPYCKKVNIEVLDDVLKDMKLKVKPQL
                     IECSKIASPMMIGFLKPMILLPDNDYSNNELYVVLKHELTHIRRGDIWYKLLLIAANS
                     IHWFNPLVYLMVKPANRDLEYSCDDVVVKNSDMNFRKEYSLTILKAMQKSGKDMLSAY
                     LNGGVSNE"
     gene            complement(6528..6896)
                     /locus_tag="PLA01_01290"
     CDS             complement(6528..6896)
                     /locus_tag="PLA01_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HPC99965.1"
                     /translation="MPDVKKLPDSELEIMMVVWNAEEKVTSDYIMANLDKTWQKTTVL
                     NFLTRLCDRGFLKYHKEGRLNVYEPLVNKEEYLQKESKSFLQKLHNNSLKSLVTSLYD
                     GKNISKEDLEELKKFIEEAE"
     gene            7081..7719
                     /locus_tag="PLA01_01295"
     CDS             7081..7719
                     /locus_tag="PLA01_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPC99966.1"
                     /translation="MPQETFFNLPEDKRSLIISSAVDEFSKAGYNTASINQICKKSNI
                     AKGSFYQYFKDKLDLYVYIMTMAIQEKVNFFSTAITESHDLTFIEQFRLLFIKGVEFA
                     KKRPQYAALGEQFSKENDESAKSAVIKEGDKQSEALFIQMIQSAKAKGEIDSRVDSLA
                     LSMLLQALNSSVNKYMLNKFGNVGYEYNEGNINNFVDSLLHILYNGIRNKSN"
     gene            7739..8308
                     /locus_tag="PLA01_01300"
     CDS             7739..8308
                     /locus_tag="PLA01_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99967.1"
                     /translation="MKKKIDFIFGMLFAVATIVFIVLFLTNDAFFNWAFERHHNVLSW
                     YIRPLFIIPMVIFAFKKSFTGVFASIFALFTSMFWFSVPAANDPKVLEFLAFEMEYLK
                     GAWTAPKILMSLAVPLFFFMLLTAAWKRNWKWLLGVIIGAAVLKFVWSIAFSGEAGMS
                     ILKPALLGLIVCIGGLGYYFKKHKRSDKN"
     gene            complement(8421..8987)
                     /locus_tag="PLA01_01305"
                     /pseudo
     CDS             complement(8421..8987)
                     /locus_tag="PLA01_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944729.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
     gene            complement(9205..9339)
                     /locus_tag="PLA01_01310"
     CDS             complement(9205..9339)
                     /locus_tag="PLA01_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99968.1"
                     /translation="MQMVSGIFLLNENETFFSAVLSENEDYNKQLGIDKLKQQSAADL
                     "
     gene            complement(9363..>10154)
                     /locus_tag="PLA01_01315"
     CDS             complement(9363..>10154)
                     /locus_tag="PLA01_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009214420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPC99969.1"
                     /translation="AGYRDVLATIHESHDYIPPRPSIILQMHRGLYKFSGLSFGGRYK
                     SSDNVIAEEDESGNKFVRFQPISAWETPEAIAQICKSYEQALDTGKVDPLLIIPMFIL
                     DFLCIHPFNDGNGRISRLLTLLLLYRSGYIVGKYISIEKLIEQSKETYYEALQHSSAD
                     WHAGTNDYASFVKYMLGVIVNAYREFSSRVKLLNTSGLSKPDRVREIIKGTLGKITKT
                     EILQKCPDISQVTVQRALNDLLKKGEIIKLSGGRYTSYVWNGEKD"
BASE COUNT         2964 a         2145 c         1685 g         3360 t
ORIGIN      
        1 aatgatataa ttttacccag aaaacggccg tataccatat cgttaatttg aaccaagttc
       61 atccccataa acctttttgc aagcattaca taggatagaa aaaacggagt cgaaacagcc
      121 agtcctgcca atataaccat ccatgtttgg tgttcggtta ttcctgccgt aaaatccgtt
      181 aatagaactg aaccctgaac aaaaccggca attaagaaaa gtaattgctg actgctgatt
      241 tttccttttt caacactcat attatttccc cccttcactt tgccatgccg gttttttcaa
      301 taaatcggtt tttattatct ttgcttcaac gtcaattttt aattcaacag aaggatataa
      361 atcatcccaa ttgtctttta aagctttcca ctcgttgctg tattttttat gcagcatttc
      421 tccaaaacca aaaatgtcag catttaattc cttggattta tcgaaagccg ccagtatttc
      481 ctgacgtatt atttcttccg aagctttttg cattttctca aaggccggat cggttgccag
      541 gttttcggtt gtagtctgtt ccgaaaggct cgattcctct tttattttta tatgcattac
      601 tatttttcca tctctcaatt tcggcgttac tttgctttcg gcttctaaaa tctccaaaac
      661 agcacttcct tgttcattag gtgaacaaac ctgtatcacg ccgcttttca ctttaccgag
      721 tacccacaaa agtccccgga cttcagcctg gtttagcttg ccaaccattt tgcccttatt
      781 gaaaacagcc aaacccgaca cataaatatc cttgttccca ttgtcctgca aaacactgac
      841 taaaggtgct accggagctg tagtagtgct catcaggcgt gaagcaaaat ctttcatatt
      901 cactttatat aattgagagg taaaaacata ggactcgacc agcttttcaa tattggcagc
      961 aggaaacttt tcagttttcg gtttggtttc caaaacgtcc gaagcccgat cctcggcaac
     1021 aagaatcaat gcagtcggtc tcatttcatg ggcacgcaaa aagaagtcaa tatacttttc
     1081 caaaccctct gaagctatat cttccccata aatcacagct tgattgtggg atatgaacag
     1141 cctgtttcct gttttgtgtg ttatttgcct tactgcatca aaaattgaat ttcccgtgct
     1201 ggtaacattc caaaatgccc cgttttcccc tccaccgttt ttttctgtat ttccgttttc
     1261 ctcctctcta actatctgtg ctgtcagcaa aatgtttccc gtgttctcgt ccttgtcaac
     1321 tcctattcct acgacaattt ccagatcgtt tatttccctt cctccaaaac atccggataa
     1381 ggtaaacaac ataaaaaagc ataaaatcaa tttcataatc ggtaaattca tcttttttct
     1441 catcgcagcc cgctcctttt atgtagaatc ttagttgtct tccttatatg ttaacgacat
     1501 cagcccggat tatggttata tgaatcgaaa ccgccataaa caatagcttt tggacgcttt
     1561 tttaattttc gaaggggcat ccttataaaa acatccctca aacctgaaaa atcaaatggt
     1621 acaaccggcc agaaatacgg tactccgaaa gaccttaaag atgccagatg aatcagcgtt
     1681 accaaaagac ccataattac accaaaacct ccggcaagtc ctgcaagtgc aagatatata
     1741 tacctaagaa tcgttccgga atcagtctgt gccggaacgg taaagcttgc aatggctgtt
     1801 gcagacacga caattaccat aataggactt ataagcccgg cctcaacgga tgcctgccct
     1861 agtacaattg ctccgacaat accgattgtt tgtcctaccg atttcggcaa tcgtactcct
     1921 gcttctctta aaatgtcaaa ggtaaacatc atcaaaaaag cttccaaaat tgccggaaaa
     1981 ggtacccttt cacgtccttc cgctatggat atcaatagct gtgtaggtat catctcctgg
     2041 tggaaaacag tcaatgccac ataaattgca ggtcctaaaa tactgatgat ataggacaaa
     2101 aaccttacta tacgcataat gctcgcatga aacggcctgg aataatagtc ttcactgctt
     2161 tgaaagcttt caataaacag catgggtact ataagaacaa aaggtgtacc gtcaaccaaa
     2221 atggcaactc ggccttcaag tattttggct gcaaccttgt caggtttttc gctgtttgaa
     2281 acagtaggaa aaatagagta ggaactatct tcaataaact gttcaatata ccctgattcc
     2341 agaatagcat ctatttttat tctgttcagc cgtctactga cttcttcaat cagtttcgga
     2401 tttgctacac tttttatata tacaatgtaa acatccgtct ttgatttgtc accgataatc
     2461 attttttcta cacataaatc gggaacttta atttttcgtc gaataagagc aatgcttaca
     2521 tttacgtttt cattgaagcc ttccctgggc ccgcgtacaa caacctctgt tgccggttcg
     2581 tctatagcac gggatctacg ctgcccggaa ttgataacca aagcctcttt tgatctgtct
     2641 atcaatagaa tggttgaccc tgccaataaa ttgtcaagca aatcatttag cagtgtcact
     2701 ttcaggctat ccgttatagg gatcaagttc tttcttacgg aatcaatgct tgcaaagtcc
     2761 atatccgcat ccttcttgag cattaatgtg ccgtacatta aaggctgcag tatgttttta
     2821 tcgatttctc ccttatctgc catgctattg ataaaaatca cggcaccttt gaatcttctg
     2881 tcatgcccga agttgaactc gtgtattgtc aaatcgctgc tgttatgcaa aatactttta
     2941 atgacttcca gattcctttt taagtcaggg aatatccgtt cactattgtt cttacccggt
     3001 aaagtatcct gatgcatgcg ttttttaata ttttttaata atgaacagcg aattttctta
     3061 taaatatatc tgaacataaa taatccttcc caagtagttt tatttatttt ttgcatacat
     3121 cagcaattta ttcataaact gaaaaataaa gactctacat tgaacaataa aaaaaccggc
     3181 gtcaactgaa aaacagaaga cgccggactt tctctctatc aattttaaag aactaaagac
     3241 ggtgctgtgt aaacccttgc tttcatttca ttatccaaca tatacaagcc tttgggatca
     3301 cctgcaaaca agtcatattt cttgatgtta tcatcggcat tcttttcttc ttcgccttgt
     3361 tcactaatga accaatcaag gaactgcatt gtacggaaat ctttaatttt cattgcagct
     3421 tcatatatat tgttaatgga agcagttaca tacttttcgt gttcataagc ttttaccaat
     3481 ggatctttat aattgttgta tttctcattg ggagcggcaa tttccgttaa gatcaccgat
     3541 tctccattat tcaacaaata ttgacggaac aacatagcgt gatcccgttc ttcctgtgcc
     3601 tgtacataaa accaattttc aaatccgctt agattttgat ctgcataata atttgccatg
     3661 tccaaatata aatatgcaga atagaattct ttattaacct gctcattcaa taatttaact
     3721 atttcctgat taagcattac atctcatcct ttctttattt taatttattt tgccaattcc
     3781 aaaaaaatct ttttgtttag gtgcccatat ccaattatat aattagcatt taaagtactg
     3841 acaacttttt aattaatctt accaaatact ctatgaaccg tcaaatgctt ttgccgtatt
     3901 tgttcgtttt ttacagtagt tccggtttgg caacaattat catcttttat attataccct
     3961 gtctgcgata ttaaatttat acggtcatag gtgtgggaga gtgtatttta gtttgctttg
     4021 gagttctttt caccttgcaa acagcttaat cagtaattct agtaaccggc attattgtgt
     4081 tcatacaagg taatcatgat atcccttgca atcatttcgt tttccatgct ttttaaatcc
     4141 ttatacgcaa tcatatttgt gccttcaaaa acaaatatat acggtgagta tgtatattcc
     4201 cggctgctgt cctctgcagt ttctatgcca taaatcaaga cctttaaatc atgcccgata
     4261 taatcgtata tattaatccc aagctttttg gcctcttgtt ctaaattaaa atcatctccg
     4321 taatcgttat tcttgtaatc cgtcttgtct gtaacacgga tgttccagcc catgccactg
     4381 tttactgcaa tctgcttacc cttcggtatt acaccgcgaa ttttatccat cataattgta
     4441 ttatcgtcga caacccaaat tccaccgtga tcttttttaa ttggtctatg aaaatggata
     4501 acagaatctt cattttcgtc acttgtatat gttaccatgc tcttatcatc gtcagaatgg
     4561 gttatctcaa aggtaccccc actctttttt aaggtattat aaacataatc ctgtgccacc
     4621 tccaacgggt taagacgcca aagctgccgg ccgttgtcaa cctctttttg catatcggta
     4681 taggacagat caatattata tgggattaat tcataagaaa tgtcggaaat cttaacccat
     4741 ttgccgtcat ctccccctac caaattaacc tctgcccatt ctccttgacg tttaattata
     4801 cgaaatacat ttttatgacc ttcttgatat atatcctttt cattggtcga tttgtagatc
     4861 ttcccgtttt taacgatgcc gaaatttgca ttgtcaaata aatttttatc aaaggaaact
     4921 gcttttttag aaatatatcc ttctgtcgga ggaatactca ttacaggaag ctgaacataa
     4981 tagaaatcat cgttttcatc ataaattaca tacacaaggt cattcttcat aagttcaaca
     5041 ccgacaccgt ttccctgggg atcggcaagc aaatcggtat cttctgttac aatagcagta
     5101 ccagtgcccg ttatatttgc cgttacacgc cgtatgctct tttttatttt ttcattatct
     5161 tctgacatct tcagatagtc atccgccaat tctttattgc cgccatccca tatcggatac
     5221 tgaacatcgg tgggcatacc gaaaacatag gtatatccgt ccccctgcaa agcaattgag
     5281 cgattcccag ggtctgtaat atcctcatgg gttaatttat caccttttaa catttctatg
     5341 taaaacaaca atcctgtgcc ttctccgtat tccttgcgga tagccttatg ataaacatat
     5401 actatattag catttctttc ttcaatttca tatttgtttt cccagcttct cggaagctca
     5461 aaacagcaat caaaggtatt gcaaaaatat gttatattct tgccggaatt aaaacccacc
     5521 aacattccga ctacaccgac aaaaatcagc atcgaaacaa aagccaacgc ccctgtcttc
     5581 ttcgctttaa tatttaaaat attgtcaaat ctctttttcg cccttttccc gttttcggaa
     5641 aaacttgtgg aaagagcagg cggttttaaa attttattca ttcgaaacac ctccgtttaa
     5701 ataggcagaa agcatatctt tcccgctttt ttgcattgct tttaaaatag tcagagaata
     5761 ttcttttcta aaattcatgt cactgttttt taccacaaca tcatcgcacg aatactccag
     5821 atcacggttt gctggcttta ccattaaata tactagggga ttgaaccaat gaatggaatt
     5881 tgccgcaatg agcaaaagct tataccagat atctcccctt cttatgtggg taagctcatg
     5941 ttttaacaca acataaagct cattgtttga ataatcgttg tccggtaata aaatcatagg
     6001 ctttaaaaat cctatcatca tcgggcttgc tatcttactg cattctataa gctgcggttt
     6061 gactttaagc ttcatatcct tcagtacatc atccaaaacc tcaatattaa ccttcttaca
     6121 atagggcttg atttttttct tgaagattat gtagtttata atgtgatata ggaaaaaacc
     6181 gacagcacct gttccccaaa caacagccaa taactcctgc aatgtaatga ccggtgcgta
     6241 gtcggcagaa acaggcgaag tattggcttt atgggtatag ctgtcatccg taaccataat
     6301 aggtactcct tcttgcctga aaaccactgt ttgattggga actgatatgt ttacaggggc
     6361 ttcaggcaat tcaatcctta ccggaataat taatcttatg gctataatca gccatataaa
     6421 ataacgccat tttgcactgt aacgtttgtt taaaaggggc gaaacaatca gcagcaacaa
     6481 gataactgcc gacatggaaa gtgaaatagc aaatatgttt ttcagcatta ctccgcctcc
     6541 tcaatgaact ttttcagttc ctccaaatcc tcttttgaaa tgtttttgcc gtcatacaat
     6601 gatgtaacaa ggcttttgag tgaattgtta tgtagtttct gtaaaaatga cttgctttcc
     6661 ttctgcaggt attcctcttt atttacaagg ggctcgtaaa cattaagcct gccctccttg
     6721 tggtatttta gaaaaccacg gtcacacaaa cgggtaagaa aattaagcac agtggttttc
     6781 tgccaagtct tatcaaggtt ggccataata taatccgatg ttaccttttc ctcagcattc
     6841 cagacaacca tcataatttc aagctcgctg tcgggaagtt tttttacgtc cggcattttt
     6901 atctctcctt gttttacatt tgtcgaatct attttaattt tacaattgta aaatatcttt
     6961 gtcaagatat tatgaatgaa aattcacata tatttttatg tttgacaata tgaccatgtg
     7021 gtcgtataat ataagtaggg atatgaccat gtggtcactt atctttgaaa ggaggttctt
     7081 atgccgcaag aaaccttttt taacttgccg gaggataaaa gaagcttgat aatctcatct
     7141 gctgtggacg aattctctaa agccggctat aatacagcaa gtataaatca aatatgcaag
     7201 aaatcaaaca ttgccaaagg gagcttctat caatatttca aagataagct ggatttgtac
     7261 gtttatataa tgactatggc catccaagaa aaagtcaact ttttctccac tgctataaca
     7321 gaatctcatg acttaacttt catagaacag tttcgattgc tttttatcaa aggtgtagaa
     7381 tttgcaaaaa aacgccctca atatgcagct ttaggcgaac aattctccaa agaaaatgat
     7441 gaatctgcta aatcagcagt aataaaggaa ggagacaaac aatcggaagc cctgtttata
     7501 caaatgatac aaagtgcaaa agctaaaggt gaaatagaca gcagggttga ctcattagcc
     7561 ttaagtatgt tactgcaagc cttaaacagt tcagttaata agtatatgct taacaagttc
     7621 ggcaatgtcg gttacgaata taacgaagga aatattaata attttgtaga ttccctctta
     7681 cacatactat ataacggaat ccggaataaa agtaattaaa aatataagga ggcttgccat
     7741 gaaaaagaaa atagatttta tattcggaat gttatttgca gttgctacca tagtatttat
     7801 cgtattgttt ctaaccaatg atgcgttttt taattgggca tttgaaagac atcataatgt
     7861 attaagctgg tatataagac ccttgtttat aataccgatg gttatatttg catttaagaa
     7921 aagctttacc ggtgtattcg cttcaatttt tgccctgttt acaagtatgt tctggttttc
     7981 cgtacctgcc gcaaacgatc ctaaagtttt agagttttta gcttttgaaa tggaatacct
     8041 caaaggtgca tggaccgctc ccaaaatact tatgagtttg gcagtaccct tgtttttctt
     8101 tatgcttctt acagcagcat ggaaaagaaa ctggaaatgg cttttaggtg ttatcatagg
     8161 ggcagctgta ttaaaatttg tttggagtat tgcctttagc ggtgaagcag gcatgtctat
     8221 tctaaaacct gcattattgg gtttgattgt ctgtatcggt ggattgggtt attattttaa
     8281 gaagcataag cgttcggata aaaactaata gtattatttg ttttcaaagc caatgatagc
     8341 actgctattg ctagctattt atatttcctc caatgtttgc ttactacttt ttctattaca
     8401 tacaacggca gattaacaag tcaatcttcc tttttatagt cggccatgga tgttcgtatt
     8461 gatatttccg gattgtattt atccttatat acccgcaagc tcttggcttt aagattgacc
     8521 tctgctttga cttcaatcgg aatgatctgt tctccggtat cgataacaaa atcaacctca
     8581 caagaaccac ggtcattagt gtagtaataa acacctaaat catttattgt tttcaactgt
     8641 tggcatacat attgctctgt cagagagccc ttgaactcgg taaacaaatc gttcccgtca
     8701 agaagtgtat gctggtgcaa ataggtttgt ttgaaagagg taatcatctg gtagtcctgc
     8761 ttgtccagca gttctgcgaa gcgttctttc ctggttgcca tcaaaaattc cttgaaggac
     8821 agcggataga gcttcatgaa atccacttta cctaccggaa acgatgtgcc ttgatgcaaa
     8881 gcgattccga gaagtgaatt ggcacagaca atgtgatatt gcggtgcgtt ctcataaaaa
     8941 tatttcagtg atgatagtac tctctgcacc tcctaagata atacgactat agtatagcac
     9001 acgatttcac aaattacaac gaattttgtg tcaatcaata cactttttac aacgaatttt
     9061 tgattaacgc tacacttttt acaacgtata tagcagatat aaatgttaaa ccgaattaaa
     9121 caggcccgtt acacgatttt tcattgtgta tcaggcctaa tttcttttat tcatcttgtt
     9181 ctcactcgac taaattaaaa aaaattacaa gtcagcggca gattgttgct ttaatttatc
     9241 tatgccaagc tgcttattat agtcctcatt ttccgaaagc acggcactga agaaggtttc
     9301 attctcattt agtaaaaata tcccactaac catctgcatt tttcagtttg cctgtgatcc
     9361 ccttaatctt tctcaccatt ccaaacatat gaggtgtatc gcccgccgct caatttgata
     9421 atctcgcctt ttttcagcag atcattcaaa gctctttgta ctgtaacctg gctgatgtcc
     9481 gggcattttt gcaatatttc ggtttttgtg atttttccaa gtgtgccctt gatgatttca
     9541 cgaacgcggt ccggttttga caaaccactc gtatttaaga gctttacacg cgaggaaaac
     9601 tcccggtagg catttactat aacaccgagc atatatttta cgaatgatgc ataatcgttt
     9661 gtccctgcat gccagtcggc agaactatgc tgtaatgcct cgtaataggt ttctttggat
     9721 tgttcaatca gtttttcaat actgatatat ttgccgacga tatatccgga ccgatagagc
     9781 aaaagcagcg tcagcaaacg gcttatgcgt ccgttgccat cgttgaacgg atgaatacaa
     9841 agaaaatcca ggatgaacat cggaataatt aaaaggggat cgacttttcc ggtgtccaga
     9901 gcttgctcgt acgatttaca aatttgtgcg atagcctccg gtgtctccca tgctgaaatc
     9961 ggctggaaac ggacgaattt gttgccggat tcatcttctt cggctatgac attatcggaa
    10021 cttttatatc ttcctccgaa agaaagaccg ctgaatttat ataaaccacg atgcatttgc
    10081 agaatgatcg atggtctcgg tgggatataa tcatggcttt cgtgaatggt ggccaaaacg
    10141 tctctgtaac cggc
//
LOCUS       DAOOJC010000024        10141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_5915_length_10141_cov_3.762344, whole genome shotgun sequence.
ACCESSION   DAOOJC010000024 DAOOJC010000000
VERSION     DAOOJC010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10141
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5915_length_10141_cov_3.762344"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /gene="radA"
                     /locus_tag="PLA01_01320"
     CDS             <1..1083
                     /gene="radA"
                     /locus_tag="PLA01_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPC99970.1"
                     /translation="DPGIGKSTLLLQICDKVKVSSKVLYISGEESIKQIKIRADRLNV
                     RNPNLLMVSETNFKVIQALCESERPDLIIVDSIQTMFNDELSAAPGSVSQVREITAGL
                     MRIAKTMNIAVVIVGHVTKEGAIAGPRVLEHMVDTVLYFEGERHLSYRVLRAVKNRFG
                     STNEIGIFEMRDIGLVEIDNPSSMMLSERTESVPGSVVVSSLEGTRPMLIEIQALVCP
                     TGFGMPRRMATGIDYNRITMLMAVLEKRIGMQLHSFDAYVNVVGGIRIDEPACDLGVV
                     AAIASSFRNVPVDLNTVVIGEVGLTGEVRAVSQIDKRAMEAMRIGFKNCIVPAGNKKM
                     IEQMKDLKNISVKYVENVHEALSILL"
     gene            1111..2184
                     /gene="disA"
                     /locus_tag="PLA01_01325"
     CDS             1111..2184
                     /gene="disA"
                     /locus_tag="PLA01_01325"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HPC99971.1"
                     /translation="MDKSKDDEVLGVLRMLAPGTPLREGLENILKARTGALIVIGDSE
                     KVLKLVDGGFYINKDYSPAYIYELAKMDGAIIVSKDLKKILYSNALLIPDSSIPTSET
                     GTRHKTADRVAKQTGEVVIGISQRRNIITIYMESGKYILRETPVILAEANQALQTLEK
                     YRAVLNDAINNLSVLEIEDIVTLDDVAFVLQRTEMVISVAAEIERYICELGSEGRLLS
                     MQLDELLSNVEDDELLVIEDYMQDTVETPERILERIRQMSYDDLMNLENICRILGYKG
                     GNNVFDITVHPKGYRLLSMVPRLPISIVKNITETFSNFQGILNASIEELDDVDGVGEV
                     RARLIKEGLRRIQDQIFLDTRRL"
     gene            complement(2311..2706)
                     /locus_tag="PLA01_01330"
     CDS             complement(2311..2706)
                     /locus_tag="PLA01_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPC99972.1"
                     /translation="MKDIMVDQFQYTVGELLVRNKSILDLITKFQDSNGRVNREIVKA
                     VTQCGCVKINATKQIYPDGADFDEIRMLMETHLEGSLCENCRDLIEKDIGRNMFYLAS
                     ICNTLDLNLYDIILKELDRIKMLGKYNLR"
     gene            3164..3598
                     /gene="rplM"
                     /locus_tag="PLA01_01335"
     CDS             3164..3598
                     /gene="rplM"
                     /locus_tag="PLA01_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPC99973.1"
                     /translation="MKTFMAKANEVERKWYVVDAEGKPLGRLASEVAKILRGKNKPNF
                     TPHVDTGDFVIVLNAEKAVLTGKKMDQKLYRRHSGYPGGLKEIKYRAFMSDKPEKAIE
                     IAVKGMLPKNSLGRAIGKKLKVYRGTEHPHEAQKPETLEINI"
     gene            3622..4014
                     /gene="rpsI"
                     /locus_tag="PLA01_01340"
     CDS             3622..4014
                     /gene="rpsI"
                     /locus_tag="PLA01_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPC99974.1"
                     /translation="MAKVQYYGTGRRKKSVARVRLVPGNGKITINDRELDEYFGLETL
                     KVIVKQPLMLTDTVSKFDVLCKVIGGGFTGQAGAIRHGIARALLMADEELRPALKKAG
                     FLTRDSRMKERKKYGLKKARRAPQFSKR"
     gene            4272..4625
                     /locus_tag="PLA01_01345"
     CDS             4272..4625
                     /locus_tag="PLA01_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HPC99975.1"
                     /translation="MTAHKIYKMSFSKVYPLYVSKAEKKGRTKSEVDEIICWLTGYYQ
                     EELEAQLEKETDFETFFKEAPQLNPLRVLIKGVICGVRVEDIEDPMMQNIRYLDKLVD
                     ELAKGKAMDKILRKE"
     gene            4727..4870
                     /locus_tag="PLA01_01350"
     CDS             4727..4870
                     /locus_tag="PLA01_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99976.1"
                     /translation="MNYRNRYLKGIYGGIPITRDINFDELLEIMEWEGDPEIVAAYAE
                     LQD"
     gene            5009..5968
                     /locus_tag="PLA01_01355"
     CDS             5009..5968
                     /locus_tag="PLA01_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HPC99977.1"
                     /translation="MSSKRFFSVFMVCLLVAMLVGAIIPGNVLAETSGKTVVLQVDNT
                     AAVVNGENVTLDVAPYIDPSSGRTLVPLRFVSESLGYTVFWDAEEKAVRVYNKIDMLT
                     LDELGDSLEDSLEFFRSISTYKYVKLTIGSNIAETCDEYLIGEYNFMKDVTIDQAAII
                     KDGRTMVPVRFISEQMGLDVGWDGSTRKITITSQGEGYVPELIEAALNENAGPYSGSD
                     FDFEQDPAHIKSQQPENYLMKIGTDGLVYYIDLAVKSSGTDEFVLSGEVTGMRGNTAC
                     FGYALSGYDGYWSDGIIKVTDEGFVVNYTNENGEKCSITFPKK"
     gene            6453..6653
                     /locus_tag="PLA01_01360"
     CDS             6453..6653
                     /locus_tag="PLA01_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99978.1"
                     /translation="MFWKNENLLTAKTRLTYASCIITAILALIYSLFFGQGDGLPEYV
                     RTLGILSVISTVVLRQNEEFRG"
     gene            6838..8220
                     /locus_tag="PLA01_01365"
     CDS             6838..8220
                     /locus_tag="PLA01_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPC99979.1"
                     /translation="MKNPNNNSDKGFYIKLFAIGLPIAFENFMLSMVSASDAIMLGAI
                     EQSALSAVSLAGQVQFVQILFIFAIIIGTSMFAAQYWGKGEKATVEMIFAFVLKLSVG
                     ISIIFWIATALFPTYIMRIFTSEYDLIQKGAEYLRVVGLSYLFVGISEMYLCIMKNCG
                     KAFISMVISSVSVVMNIVLNAILIFGYFGAPKMGIAGAALATVISKGFETAWLVFLSF
                     RKDGIRLKTKYLVRTNKQLQKDFWKHTLPVFGNQMVWGIGFTMYSVIMGHLGNDAVAA
                     NSIANIAKNLISSFCLGLGNGGSIIVGNVLGSGNLEEAKRYRGKVIKMAIVSGIITGG
                     VLLGLIPAILHFTNLSEQATEYLKWMLVMCSYYLVGKSINATTIPGIFCAGGDSKFGF
                     ICDAITMWAVTVPLGFFTAFVLRLPVLAVYFVLTLDEIVKLPAVYKHYKKYGWVRDLT
                     RNLEQEALVS"
     gene            8416..8886
                     /locus_tag="PLA01_01370"
     CDS             8416..8886
                     /locus_tag="PLA01_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99980.1"
                     /translation="MKRKLVVTVISLVLVLCSVQVYAVTVLNTNLINLIRNAFNTIED
                     EYETVVEADVDNLSDKYKTETEKYVNEMAQDVFDEIEVHRNNEVSRIDQELNNYLDTL
                     KSELNSVTDQQVSEVKDSITEKIDSTVEKLKKDIEKDLEKQLKDFLKDIRKNDN"
     gene            9209..>10141
                     /locus_tag="PLA01_01375"
     CDS             9209..>10141
                     /locus_tag="PLA01_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPC99981.1"
                     /translation="MIKLKKLMRLVLILALVTILSATSFAKEDSVKLFSDVPENHWSY
                     KAIHDLRSLGITDGIGNNRFGMGQTIKRSEFVTFLTKLMNWELIKPEKGSFVDNQDPA
                     KWYYAPVETALKHGTILKDTERFRPDDPINREEMAIMIVRTLGYNSLAGQINNSKSSF
                     DDVTENTGYIAIARDFGIISGVGNNLFKPYDTAKREEAAVMMMKMYEKLNIKIKELHA
                     FYAIHSAHQMDKISSLDSVSFGWSRLEYDVQSKQVFLNTTGRNNNQFSFPEGFTGPLN
                     KAKEYKTSTQLMVFASNNTVFSTETKSNIPLVEYI"
BASE COUNT         3321 a         1506 c         2302 g         3012 t
ORIGIN      
        1 gaccccggta taggcaagtc aacccttttg cttcagatat gtgacaaggt gaaggtcagt
       61 tccaaggtcc tgtatatttc cggagaggaa tctattaagc aaattaaaat tagagccgat
      121 agattaaatg taagaaaccc gaatctgtta atggtatccg aaaccaattt caaggttata
      181 caggctttgt gcgagagtga aagacccgac cttattattg tcgattcaat tcagaccatg
      241 tttaacgatg aattatccgc tgctccggga agtgtgagcc aggtaagaga aattactgca
      301 ggccttatga gaattgccaa gaccatgaat attgcggtag ttatcgtagg tcatgttaca
      361 aaagagggag ccattgcggg accgagagta ttggaacaca tggtggatac ggtgttgtat
      421 tttgagggag aaaggcattt gagctatcgg gttttgcggg cggtaaaaaa ccgctttggt
      481 tctacaaacg aaataggcat attcgaaatg agggatatag gtcttgtgga aatagacaat
      541 ccttcttcga tgatgttatc ggaaagaacc gaaagtgtac ccgggtctgt tgtggtttca
      601 agtcttgagg gaacaagacc catgcttatt gagatacagg cactggtatg tcccaccggc
      661 tttgggatgc caagaagaat ggcaacgggt atagattata acagaattac aatgctgatg
      721 gctgttcttg aaaaaaggat tggcatgcag ctccatagct ttgatgccta tgtgaatgtg
      781 gtgggtggaa taagaattga cgaacctgca tgcgatctgg gagtagttgc ggcaattgcc
      841 tcgagctttc gaaatgtgcc ggttgattta aacacagttg taattggaga agtaggactg
      901 acaggagagg tgagggctgt aagccagata gacaaaagag caatggaagc catgagaatt
      961 gggtttaaga attgcatagt gcctgccgga aacaaaaaaa tgattgagca gatgaaggac
     1021 ctgaagaata taagcgtaaa atacgtagaa aatgtgcatg aagcattgag cattctttta
     1081 taataaacca ttggagggct tatgataggc attgataaat caaaggacga tgaggtgctt
     1141 ggtgtattga gaatgctggc acccgggacg ccgcttcggg aagggcttga aaacattttg
     1201 aaggcacgga cgggagcact tattgttatt ggagactccg agaaggtttt aaagttagtg
     1261 gacggaggct tttatatcaa taaggattat tcacctgcct atatttatga gctggcaaaa
     1321 atggatggag ccataattgt cagcaaggat ctgaagaaaa tattgtattc caatgcattg
     1381 cttattcccg attcttcaat ccctacgagt gagaccggaa caaggcataa aaccgctgat
     1441 agagtggcaa agcagacggg tgaagtggtt ataggtattt cccagagaag aaatattata
     1501 accatttata tggagtcggg taaatatatt ttaagggaga ctcccgttat tcttgcagaa
     1561 gccaatcagg cattgcaaac cttggaaaaa tacagggcag ttttgaacga tgcaataaac
     1621 aatctcagtg tattggagat tgaagatatt gttacattgg acgatgtggc atttgttctg
     1681 cagaggactg aaatggtaat aagcgttgcc gcagaaattg agagatatat atgtgaattg
     1741 ggcagtgagg gaagactttt gagtatgcaa ctcgatgagc ttctgtcaaa tgttgaggat
     1801 gatgagctgt tagtaattga agattatatg caggatacgg ttgaaactcc tgaaagaata
     1861 ctcgagagaa taaggcagat gtcctacgat gaccttatga atcttgaaaa tatatgccgg
     1921 attctcggat acaagggcgg gaacaatgtt tttgacatca cggtacatcc gaaaggatac
     1981 aggttgctta gcatggtacc aagactcccg attagtattg tgaaaaacat cactgaaacg
     2041 ttttcgaact ttcagggaat tctaaatgct tcgattgaag agctcgatga tgttgacggg
     2101 gtaggagagg tacgggcaag actaatcaag gaaggtctca gaagaattca ggatcagatt
     2161 tttctagata cgcgaagatt ataatgagta ttaaaaaaga gtcggcacaa catattgcag
     2221 cgactcttta taatttcaga actttttttg ctaaagccgg acaagctcat cggtaataat
     2281 tcaataaacg accggtaaat cgggttaact ttatctcaag ttatatttac caagcatttt
     2341 aatcctatca agttccttaa gtataatgtc atacaggtta agatccagag tattgcatat
     2401 tgaagcaaga taaaacatat tccttccgat atccttttca atcagatccc tgcagttttc
     2461 acagagactc ccctcaaggt gggtttccat cagcatgcga atctcatcaa aatctgctcc
     2521 gtcagggtaa atctgctttg tagcattgat tttaacgcat ccgcactggg tcacagcctt
     2581 tactatttcg cggttaaccc tgccgtttga atcttgaaac tttgtaatca gatccagaat
     2641 actcttattc cttaccagaa gttctccaac agtatattgg aattgatcta ccataatgtc
     2701 cttcaatatg ctcactcctt aggtgtattg ataaaactaa cagaatttca ataggtaaaa
     2761 gcaaaacaaa gaagattaag gaaccaactt gatagcttta tactaattat aattctttgt
     2821 gcaactcttt gtcaattaaa aatgggctgg ataaacttag caattgatgg ggcgtacaaa
     2881 gcaccggact aaggaatcta aaaaattatg agcatcggaa ggcttgcatt ttataggaat
     2941 cgaaaaatga aatgacacac gattatacgg aagcaagaag taattattgg gcaaaaataa
     3001 gagtaaacaa aagataaatc taaattttaa taaaatatac ttgacacgcc aattaacctg
     3061 tattacaatg tttaagtgtc gaaattttgg ttcactgtat ttggactaaa gtatttgttt
     3121 ctataaatta aactgtattt cgaataagga ggttgaagat tccatgaaga cttttatggc
     3181 gaaagccaat gaagttgaaa gaaagtggta tgtggttgat gcggaaggta aaccgttagg
     3241 aaggctggct agcgaagtag ccaaaatttt aagagggaaa aataaaccca attttactcc
     3301 ccatgttgat acaggcgatt ttgtaatcgt attgaatgct gaaaaggctg ttctgacagg
     3361 taagaaaatg gaccagaagc tttacagacg ccattcaggg tatcccggag gattaaaaga
     3421 aataaagtac agagccttta tgagcgataa gccggaaaag gctatagaaa ttgccgttaa
     3481 gggtatgctt ccgaagaaca gtctcggaag agcaataggt aagaagctta aggtttatag
     3541 aggaaccgag catccacatg aagcacaaaa accagaaacg ctggaaatta atatttaatg
     3601 ggaagggagg aataggtttt tatggcaaag gtacagtatt acggaaccgg aagaagaaag
     3661 aagtcagttg cgagagttag acttgttcca ggtaatggga agattactat aaatgatagg
     3721 gaattagatg aatattttgg ccttgagact ttaaaggtta tagttaaaca gccgttgatg
     3781 ctgacggaca cagtaagcaa gtttgatgtg ctttgcaagg tgatcggagg cggttttaca
     3841 ggccaggcag gtgctataag acatggtatt gcaagggcac ttttaatggc tgacgaagaa
     3901 ttaaggcccg cattgaaaaa agcaggattc ctcacaaggg attcaagaat gaaggaaaga
     3961 aagaagtacg gactcaaaaa agcaagaagg gcaccacagt tctccaagag gtaatatgtt
     4021 gtctttttat gcttcgagca tgattaaatt tacaaagaat gtttacgagt ttactaaacc
     4081 gtcaggggtt aattgccttt ggcggttttt tgtttttaaa gtgaagcttt tgtgaaaggt
     4141 aatatataat tcttgacttt cacataataa tgatataata ctatgtgtcc tttttatata
     4201 gcaggtgtgg atttatgtgt gattagcaat tcttaaaatt atggatgata aataataaat
     4261 agaggtgtat tatgactgca cacaagattt ataaaatgag tttttcaaaa gtttatcccc
     4321 tttatgtttc gaaagcggag aaaaaagggc gtactaaatc agaagttgac gaaattattt
     4381 gttggttgac aggatattat caggaggagt tagaagcaca gttagaaaaa gagacggact
     4441 ttgagacatt ctttaaagaa gctccccaat taaatccttt acgggttttg attaaaggtg
     4501 taatctgtgg tgttcgagtt gaggatattg aagacccaat gatgcaaaac attcgttatt
     4561 tggataaatt agtagatgag ttagcaaagg gaaaagccat ggacaagatt ttacgaaaag
     4621 aatagtataa atagagtaaa aaatgattca tacgtttgtt gttgataaag atgcaaataa
     4681 agaattattg ccaattgtat aatttgaaga tagaactcat ccaaatttga actacaggaa
     4741 tagatattta aaaggtatat atggcggaat accgattacc agagatatta attttgatga
     4801 actgctggaa ataatggaat gggagggtga tccggaaatt gtggcggctt atgctgaatt
     4861 acaggattaa atacatttgg gaaaaaaaga atgatgttta tataatgtgg atataaacat
     4921 cattgacatt tttacgatga aaagatacta tatcattggg attttatatt agtagtttat
     4981 tttagagaaa ctatgaggag gtttttttat gagttcaaaa aggtttttta gtgtttttat
     5041 ggtatgtttg ttggtagcaa tgcttgtcgg tgcaatcatt ccaggtaatg tattggccga
     5101 aacttcagga aagacagttg tactgcaggt ggataatact gctgctgtgg ttaacggtga
     5161 aaatgtaaca ttggatgttg ctccgtatat tgacccgtcc agtggcagaa cgttggtacc
     5221 cttgagattt gtatcggaat ctttggggta tacggttttt tgggatgctg aagagaaagc
     5281 cgttcgtgtt tacaataaaa ttgatatgtt aacattggat gaattaggag attcattgga
     5341 agattcattg gaatttttca gatcaataag tacatataaa tatgttaaat tgacaatagg
     5401 cagtaatatt gccgaaacat gtgatgaata tcttattggc gaatataatt tcatgaaaga
     5461 tgtaactata gatcaagcag caattattaa agacggaaga acaatggttc ctgtaaggtt
     5521 tatttcggaa cagatgggtt tagacgttgg atgggatggc agtaccagaa agataacgat
     5581 tacatcccaa ggtgaaggat atgttcctga gcttattgag gctgcactta atgaaaatgc
     5641 aggaccttat agcggatcgg atttcgattt tgaacaagat cctgcacata taaaatccca
     5701 gcagcccgag aattatttga tgaaaatcgg cactgacggc cttgtatatt acatagactt
     5761 agccgttaaa tcatcgggaa ccgatgaatt tgtattaagc ggagaagtta cagggatgag
     5821 gggcaacaca gcatgctttg gttatgcact gagtggttat gatggttatt ggagcgacgg
     5881 aattattaag gttacagatg aaggctttgt tgtaaactac acaaatgaaa atggagaaaa
     5941 gtgttctatt actttcccca aaaagtaatt tgagaaatta attaaggtct tggcggtttt
     6001 ttagctgcca gggccttttt gtatgtaatc tcttatcaat gccagggtcg atactttttc
     6061 agcagaaata tagatgaatt tagcatttct gttttgaata agtgtaatta ataatatatt
     6121 aataaattag aatataaaaa taatatagac aatacatggc tgtaatgcta atataattat
     6181 agagtttacg taattagtaa acgttgcata taaaatgaca tataagtgca caagacagtt
     6241 gagggcagtt gttggtgttc gcagttcgta tgtttcatat taaaatcgta ttatcccttg
     6301 tcctttcata gttcttactg cctcgcaggt attagatgca gtaatttttt tctgcggatt
     6361 tatcctaaaa tgcattaaca ggaggtatac aaaatggaaa taattttgtg tgtcttgttt
     6421 tttattgtta ttgcggacgt tacgttgttt ttttgttctg gaaaaatgaa aatctattga
     6481 ctgctaaaac acggcttaca tatgcgagtt gtattataac ggcaattttg gctctcatat
     6541 acagcctttt tttcggtcaa ggcgacggtt tgcctgaata cgtcagaacg ttgggaattc
     6601 tatcggttat atccacagtt gttttaagac aaaacgaaga gtttagaggg taatggactt
     6661 agaataaaaa gaactaaaaa tgcgaatttt ttattaggag tttttcagat aaatatagta
     6721 taattatatc attacggact ttgttttatt ggtgtgacgt tcccacgaaa caaaaacact
     6781 actataaaca ttattacaag gatgaattat acgacagtga ggcaggtaat acaatctatg
     6841 aagaatccaa ataacaattc cgacaaaggt ttctacatta aactgtttgc aataggctta
     6901 ccgatagcgt ttgagaattt tatgctttca atggtcagtg catccgatgc aattatgctg
     6961 ggggctattg aacaaagtgc tctttcggcg gtatcccttg cgggacaggt tcagtttgtt
     7021 caaattctgt ttatttttgc gatcattatc ggtacaagca tgtttgccgc tcaatattgg
     7081 ggtaaaggcg aaaaggcaac ggttgaaatg atatttgcat tcgttttaaa gctttccgtt
     7141 gggatttcca tcatattttg gatagcaacg gcattgttcc ccacatatat aatgcgtatt
     7201 tttacatctg aatatgactt gatacaaaaa ggagccgaat atctacgggt agttggtctt
     7261 tcatatctgt ttgtcggtat atccgagatg tatctgtgta taatgaaaaa ttgcgggaaa
     7321 gcattcataa gcatggtcat cagttcggtt tcggttgtaa tgaatatagt actgaatgca
     7381 atacttattt tcgggtattt tggtgctccg aaaatgggaa tagccggtgc agctctggcc
     7441 acggttattt caaaaggttt tgagaccgca tggcttgtat ttttatcatt cagaaaggat
     7501 ggcataaggc ttaaaacaaa gtaccttgtc agaacgaaca agcagcttca aaaggatttc
     7561 tggaaacaca ccctgcctgt tttcggcaat caaatggtgt ggggaattgg ctttaccatg
     7621 tactctgtaa taatggggca tttgggaaac gatgccgttg cagcgaattc tattgcaaac
     7681 atagcgaaga accttatttc cagcttttgt ttgggacttg gcaacggagg atcaattatt
     7741 gttggaaatg tattgggtag cgggaacctt gaagaagcta aacgttatag gggaaaagtt
     7801 atcaaaatgg caattgtcag cggtataatc acaggtggcg tattgcttgg acttattcct
     7861 gcaatcctgc attttacaaa tttgagcgag caagcaacag agtatttgaa atggatgctt
     7921 gtgatgtgtt catattatct tgtgggcaag tcaattaatg caactacaat tccgggaatt
     7981 ttctgtgccg gaggcgattc gaaattcgga tttatttgcg atgctataac catgtgggcg
     8041 gtaactgtac ccttgggatt ttttacggcc tttgttttga ggctgcctgt gcttgcagtc
     8101 tattttgtgc ttacccttga tgaaattgtg aaactgcctg ctgtttataa gcattataaa
     8161 aagtacggct gggtcaggga cctgacaaga aacctggaac aagaggcatt agtatcataa
     8221 gcaatatccg gaacttaagt tttttgaata ttttatttat gccttagtgc ggtttttacc
     8281 gataggagat tgtgaccctt aattttccga ttgaatccag ttaaatcaaa atgatagaat
     8341 aatatttata taatattacc gactcagtaa caattgtaga agaatcttaa taccaaggat
     8401 aaacggaggg gtctcatgaa aagaaaactg gttgttacgg tgatttcttt agttctcgta
     8461 ttatgttcag ttcaagtata tgcagtaacg gttctgaata ccaatctaat caatcttatt
     8521 agaaatgcat tcaatacaat cgaagacgag tatgaaacag tggttgaagc tgatgtggat
     8581 aatctcagtg ataagtataa aactgagaca gaaaagtatg taaatgagat ggcccaggat
     8641 gtatttgatg aaattgaggt gcacaggaat aacgaggttt ctcgaataga tcaggaattg
     8701 aataattatc tggatacctt aaagagtgaa ttgaacagtg tcactgacca acaggtttcg
     8761 gaggtaaaag attcaataac ggagaaaata gacagtactg tagaaaaact gaagaaagat
     8821 atcgaaaagg atttagaaaa acagttaaag gatttcctga aagacataag aaaaaatgat
     8881 aattagattt ttgagtcggt aaaggttatt gagttgcgaa ttttgaaggg aaagtacatg
     8941 gtgcccgttt ctacgtttct atatgagggc taaagttggg gatgattata cctgctttgc
     9001 cctattcttg ttttatccat tatatcatca tttaatttct tctgtgatat caccattaaa
     9061 agtgatagaa taatatcaaa cttgatgaaa ttgatttaaa gatgaggttt ataaagataa
     9121 gaggataagc ctgttggaac tatacgaaag ataagacttc catagaggcc caatattggc
     9181 aagcaaatga aagtgaggct ctatacatat gataaagcta aaaaaattaa tgagattagt
     9241 attgatattg gctcttgtaa caatattgtc cgctacatcc tttgcgaagg aagattcagt
     9301 taaactcttt agtgatgtac ctgaaaacca ttggtcatat aaagccatac acgaccttcg
     9361 ttcactggga attaccgacg gcatcggaaa caatcggttt ggtatgggac aaacaattaa
     9421 aagaagcgaa tttgtaactt ttctgacaaa actcatgaat tgggaattga ttaagccgga
     9481 aaaaggcagt tttgttgata atcaggaccc tgccaagtgg tattatgcac ctgttgagac
     9541 ggctttaaag catggcacga tattaaagga tactgaaagg tttcggccgg atgatccgat
     9601 taacagagaa gaaatggcaa ttatgattgt tcgtactttg ggatataaca gtcttgccgg
     9661 tcagataaat aacagtaaaa gttcttttga tgatgttacg gaaaatacgg gatacatagc
     9721 tattgccaga gactttggaa ttattagcgg agtgggcaac aatcttttca aaccctatga
     9781 tactgcaaaa agggaagaag ctgcggtaat gatgatgaaa atgtatgaaa aactgaacat
     9841 caaaattaag gagctgcatg ccttttatgc aatacattcg gcccatcaga tggataaaat
     9901 ctcaagcctt gattctgtga gcttcggttg gagccgtctt gaatatgatg ttcaatcaaa
     9961 acaggtcttt ttaaatacca caggaagaaa taataatcaa ttctcattcc ctgagggatt
    10021 tacagggcct ttaaataaag ctaaagaata taagacttcg acacagctta tggtatttgc
    10081 aagcaataat acggttttta gcacagaaac taagtctaat attccgttgg tagaatacat
    10141 a
//
LOCUS       DAOOJC010000025        10052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_5972_length_10052_cov_3.750825, whole genome shotgun sequence.
ACCESSION   DAOOJC010000025 DAOOJC010000000
VERSION     DAOOJC010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10052
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5972_length_10052_cov_3.750825"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /gene="eno"
                     /locus_tag="PLA01_01380"
     CDS             <1..177
                     /gene="eno"
                     /locus_tag="PLA01_01380"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512177.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPC99982.1"
                     /translation="DATIADIAVATNAGQIKTGAPSRTDRVAKYNQLLRIEEELGDIS
                     IYPGLNAWFNLKNK"
     gene            335..574
                     /gene="secG"
                     /locus_tag="PLA01_01385"
     CDS             335..574
                     /gene="secG"
                     /locus_tag="PLA01_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPC99983.1"
                     /translation="MQIVVTIFHIIFSVSLVVIVLLQSGKQAGLSGSIAGGAETFFGK
                     NKGRTIDAILSKYTLVAAIGFIITSIVLQIVLNSK"
     gene            879..1430
                     /locus_tag="PLA01_01390"
     CDS             879..1430
                     /locus_tag="PLA01_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPC99984.1"
                     /translation="MEREQALEELKGRVKNENLIKHSLAVEAIMKELAFYFKDDIDKW
                     GLAGLLHDIDYDKTADDPSKHSLVGADILESLDVESSIVYAVRAHNDYHGIERKRKID
                     KALYCADPIAGLITASALILPSKKLEDVTVEFVLKRIDEKGFAKGANREQIRSCEQLG
                     ISLEKFVEIALQAMKMISDDLGL"
     gene            1831..4086
                     /gene="rnr"
                     /locus_tag="PLA01_01395"
     CDS             1831..4086
                     /gene="rnr"
                     /locus_tag="PLA01_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HPC99985.1"
                     /translation="MKDRKERILAFMREDAYKPLLFSELMTVLDVPKSDAGIFENLLE
                     ELEQEGSIFKTHKNRYGVPERMNLIVGYLQGNVGGYGFVIPDDANIKDIFISSENLNG
                     AMHKDRVIARVNKKVIGDKRAEGEIIRIIERANNTVVGTFESSKYFGFVVPDDTRISG
                     DIFIPKDEVNGARSGQKVVAEIVRWPEKRRNAEGRIVEIIGDRNEPGTDILSIIKAYN
                     LREDFPEDVAKQAETITQTVTGDMIKGRRDLRSLRMVTIDGEDAKDLDDAVSIEKLDN
                     GNYRLGVHIADVSYYVTEGSYMDKEALKRGTSVYLVDRVIPMLPKALSNGICSLNPGV
                     DRLAFTVMMEIDRDGRVLDHEIFESVINTNERMTYTNVYKILEEDDRELIERYRYLLD
                     DFLAMKELALILRKKRIVRGAIDFNFDEAKIKLDEKGVPVQIERYEITIANRIIEEFM
                     LVCNETVAEHFFWLNTPFVYRSHEEPDNEKMEVFSEFVHNMGYTLKGISKIHPKALQD
                     ILEKAKGKKEETVINTVMLRSLQKARYSHINDGHFGLAAKYYCHFTSPIRRYPDLIIH
                     RIMKEYLKGNIIPSREELLNERIPEIARLCSERERAAEEAERETEDLKKVEFMKKYEG
                     EEFEGIISNITSFGMFVELENTVEGLVRMSSMEDDYYVFNEKNYSLIGEKSRKVYRIG
                     DVVRVMLVRADIASRKIEFAVVDNEDLKEKESETEEQTAQETKRKRKRKVEESVLLHV
                     KGKKKVKKLKK"
     gene            4102..4557
                     /locus_tag="PLA01_01400"
     CDS             4102..4557
                     /locus_tag="PLA01_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HPC99986.1"
                     /translation="MILVSACLLGVNCKYNGTNNLNSDVIKLLSEEKLIPVCPEQLGG
                     CSTPRPPVEIIGGDGRDVLDGKGRVISKNGEDVTQNLIRGANEVLKLAKTLGIKKAIL
                     KERSPSCGTCSIYDGTFSGKTLRGSGVAAQILKKSGITVISEEDLKNGE"
     gene            complement(4613..4840)
                     /locus_tag="PLA01_01405"
     CDS             complement(4613..4840)
                     /locus_tag="PLA01_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPC99987.1"
                     /translation="MKSFNISLKSITDVKDFVNIVNKYDFDIDLTSGRYVVDAKSIMG
                     IFSLDLSKPIKVDVHSDDCSEFCEEIKNYIL"
     gene            5024..6340
                     /gene="mtaB"
                     /locus_tag="PLA01_01410"
     CDS             5024..6340
                     /gene="mtaB"
                     /locus_tag="PLA01_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPC99988.1"
                     /translation="MKRVAFCTLGCKVNQYETEALSGIFRKAGYNVVDFEEEAEVYVI
                     NTCTVTNLSDRKSRQMIRKAKKINKDSVVIVAGCYAQTAPEEVSKIEGVNMVIGTKDR
                     GRIIEYIEELAAGGDKLNFVDDVMKTREFEELGVDVYRERTRAFIKIQEGCSQFCSYC
                     IIPYARGPVRSRAEKNIIDEITGLASKGYKEVVLTGIHIASYGKDIKTTSLVDIVKKV
                     HGIEGIERIRLGSIEPTLINEEFIESIKNLEKVCPQYHLSLQSGCDRTLKRMNRKYTT
                     EDYRKSVALLRENIRDVAITTDIMVGFPGETDDEFDETYRFLQEISFAGMHVFKYSPR
                     KGTPAASYPNQVSPQKKEERSDMLINLASSMKHSFNKGYLGKVMPVLFEQEVKGMEGY
                     VEGLTPNYIRVVCEGSAGFEGNIMKVLLKEVVSDYMTGETIHKNES"
     gene            6684..6953
                     /locus_tag="PLA01_01415"
     CDS             6684..6953
                     /locus_tag="PLA01_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HPC99989.1"
                     /translation="MADNETMMFKVEKEKVNEARELLLSVYQALKEKGYNPINQIVGY
                     ILSGDPTYITNHMDARSAIRKLERDELLEEILRFYLEETANKGSV"
     gene            7006..7953
                     /locus_tag="PLA01_01420"
     CDS             7006..7953
                     /locus_tag="PLA01_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPC99990.1"
                     /translation="MDLCKLGNTGLEVSKLCFGGLIIGPLQVNLSPEQGADVILKAIE
                     LGVNFIDTAELYGTYPHIREAIKKSNKQIIIASKSYAYSKEGARDSLEKARRELDVDV
                     IDIFLLHEQESRLTLRGHREALEYYISMKEKGIIKAVGVSTHNIEVVDACAHMPEIDV
                     IHPLINKHGIGIGDGTIDAMLEAVKKAYSAGKGIYSMKPLGGGNLINSYKESMDFVLN
                     IPYIHSIALGMQSIEEVIMNVCVFENKAVPQEIQESLSKRKRRLHIDWWCEGCGKCVQ
                     RCKQGALSVVDNKARVREDKCVLCSYCASVCPVFAIKVC"
     gene            7997..8425
                     /gene="ruvX"
                     /locus_tag="PLA01_01425"
     CDS             7997..8425
                     /gene="ruvX"
                     /locus_tag="PLA01_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPC99991.1"
                     /translation="MRIMGIDYGDSRIGIAVSDPMGWTAQGLETIKWKTDIEVPLKRI
                     SQLIKEYDIQKIIVGFPKNMDGTVGFRGEKTIEFIDLLKKRTNNIEVIMWDERLSTVA
                     ANRTMHEMGVKKSKKKMVVDQIAAVYILQGYLNSIGNTTM"
     gene            8654..8944
                     /locus_tag="PLA01_01430"
     CDS             8654..8944
                     /locus_tag="PLA01_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HPC99992.1"
                     /translation="MSEERDDLVVLVDENGEETEYEYLDTIEFNENEYVVLLSVEQSN
                     NEDNMDEVVILKIVEDPDGEDSFVTIDDESEMEAVFEEFKTRMAEEFDFYEE"
     gene            9302..>10052
                     /locus_tag="PLA01_01435"
     CDS             9302..>10052
                     /locus_tag="PLA01_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HPC99993.1"
                     /translation="MFNVTMLKKMILVAMIFVSVTLLSACGKSSETDIEVVSPSPTQT
                     KVYFTEKPEYTPEPEQKVDFDLPEKGIRPYAVMIDNQGTRVLPQGGLNKAQVIYEIIV
                     EGGITRFMPVFWGEGSTMIGPVRSSRHYFLDYAMEHDAIYVHEGWSPMAKDDISKLGI
                     NNINYGVFWDLTTDKSNWQDTYTSMDKLKEFTEKVKYRTQTEKELVFKYADKDYELEN
                     GSKAEKIKLSYSWDYISEYTYDPEKKLYLRFR"
BASE COUNT         3376 a         1424 c         2426 g         2826 t
ORIGIN      
        1 gatgcaacaa ttgccgatat tgctgttgca accaatgcag gacagattaa aaccggggca
       61 ccgtcaagaa ctgaccgtgt tgctaaatac aatcaattat taagaattga agaagaatta
      121 ggtgacatta gtatttatcc cggtctcaat gcatggttca accttaagaa taaataggct
      181 aagagaatgc acgccatcgg tgtgtaacag gtaaaaagac agattacatg ggcgagggat
      241 taataaaata aaaaaacctt ggcaataaag cttgtttttt agttattatt gtgttagaat
      301 gtattctgtc aatttcttag gaggtgccta tagcatgcaa atagtagtaa ctatttttca
      361 cataatattt tcggtgtcac tagttgtgat tgttttgctt caatccggta agcaggctgg
      421 cttgtctggt tccatcgctg gtggtgcgga gacatttttc gggaaaaaca aaggaaggac
      481 aattgatgct atccttagca aatatacttt agttgcagct attggattta ttattacttc
      541 aattgtttta caaattgttt tgaattctaa ataagatcaa gaagaaagga gactgtattg
      601 tgcagtttcc tttttgtact ctttcagatg ctttattcag ctatagctga ataaagtaat
      661 tagatacaaa taagcatctt ataatttagt aataatacat tttcttatcg tatacttcaa
      721 tattttcctt gtaattattg ttctggttta gtaatataac atagagggct cacgggatta
      781 tatcctcagt gcgtatgtga attggtagtc tatcgatcag tttgatattt atcttctggt
      841 attagcggtt caataaagaa ttggaggtag tggtttcgat ggaaagagaa caggcattag
      901 aagagttaaa gggcagagta aaaaacgaaa atttaataaa gcattctctt gcggttgaag
      961 ctattatgaa ggaattggct ttttatttta aggatgacat tgacaagtgg ggattggcag
     1021 gtcttctcca tgatattgat tacgataaaa cagcagatga tccgtctaaa cacagcttgg
     1081 taggtgcgga cattcttgaa agtttggatg ttgagagctc aattgtatat gctgtaagag
     1141 cacataacga ctatcatggc attgagagaa agagaaaaat agataaagcg ttatattgtg
     1201 cggaccctat agctggtttg ataactgctt cagcattgat tcttccttct aaaaagctgg
     1261 aggatgttac tgttgagttt gtacttaaga gaattgatga aaagggcttt gcaaaaggtg
     1321 caaaccgcga acagattagg tcgtgcgaac agttggggat ttctctggag aaatttgttg
     1381 aaattgcact gcaagccatg aagatgattt ctgacgactt gggtctctaa gagcttttat
     1441 tgtatattaa aaggattgaa tcatagattt aataaacaga aaaaccaggt tgagggacaa
     1501 acttggtttt ttgtttatat ttaatctatt tatttttcta tgggtgaact tgccaggatg
     1561 agaatgaaca tgacaattct cccattcaac ataattcctt acgtgtgaaa gtattaaata
     1621 atgtactttg tgggaaaata ttttatcagg ttaattatat aagaaaagga ggaaggacgc
     1681 cgcaaattat taagacaagg catgcacact catgcatttt acagcccaag gatgctgttt
     1741 gtgattatta tcactgcatg ccagtgtagt gcgttaaaca cggattataa tataaaaatg
     1801 cggctttata caaacattat gaaggatggt atgaaggata gaaaagaaag aatactggct
     1861 tttatgaggg aggatgcata taagccttta ttgttcagtg aattgatgac tgtgttggat
     1921 gttcccaaaa gcgatgcggg aatttttgaa aacttgttag aagaactgga acaggaagga
     1981 agtatattca agacccataa gaatagatac ggagttcccg aaagaatgaa tcttatagtg
     2041 ggataccttc aaggaaatgt ggggggatat gggtttgtaa ttcctgatga tgcaaacata
     2101 aaggatattt ttatttcttc agagaacctg aacggtgcca tgcacaaaga cagggttatt
     2161 gccagagtca acaaaaaggt tatcggcgat aagagggctg aaggtgaaat aatcaggata
     2221 atagagagag caaataatac tgttgtaggg acctttgaga gcagtaagta tttcggattt
     2281 gtggttccgg atgacactcg gatttccgga gatattttta ttccgaagga tgaagtaaac
     2341 ggtgcgaggt caggacagaa ggttgtagcg gagattgttc gatggcctga gaagaggaga
     2401 aatgcagagg gcaggatagt tgaaataatt ggggacagga atgagccggg aacggatatt
     2461 ttatctataa ttaaggccta taacttaaga gaggatttcc cggaggatgt tgcaaaacag
     2521 gcggaaacaa taacccagac agtgactggg gacatgataa agggaagaag agacctaaga
     2581 agtctccgca tggttaccat tgatggagaa gatgccaagg accttgacga tgcggtttca
     2641 attgaaaagc ttgacaatgg aaattaccgg ctgggggttc atattgcaga tgtaagttat
     2701 tatgttaccg agggttcata tatggacaag gaggcactaa aaagaggtac cagtgtttat
     2761 ttggttgaca gggttatacc catgcttccg aaagcacttt ccaatggaat atgcagttta
     2821 aaccctggag ttgacaggct tgcttttacc gtaatgatgg agattgaccg tgacggcagg
     2881 gttttggatc acgaaatttt tgaaagtgta atcaatacca acgaaagaat gacttatacc
     2941 aatgtttata aaatactaga ggaggatgac agagagctta ttgaacggta cagatatctc
     3001 cttgacgatt ttctggcaat gaaggagctg gcccttatcc tgaggaaaaa gagaatcgtg
     3061 agaggagcta ttgattttaa ttttgatgaa gcaaaaatca aattggacga aaagggtgtg
     3121 ccggtacaaa tcgaaaggta tgaaatcaca atagcaaaca gaataattga agagttcatg
     3181 ctggtatgca atgaaactgt tgctgagcac tttttctggt taaatactcc gtttgtatat
     3241 agaagtcatg aagaacctga caatgagaag atggaggtat tcagtgagtt tgtgcataac
     3301 atgggttaca ccctaaaggg tattagcaaa atacatccta aagcattgca ggatattttg
     3361 gagaaggcaa agggcaaaaa ggaggagacg gtaataaaca ccgttatgtt aagatctctt
     3421 cagaaagcca gatatagtca cataaatgat ggccatttcg gcctggcagc aaaatattac
     3481 tgtcacttta cctctcctat cagaagatat cccgacctga ttattcacag gataatgaag
     3541 gagtatttaa agggaaatat aatcccgtca agggaagaac tgctcaatga aaggataccg
     3601 gaaatagcaa ggctttgttc agaaagggaa agggctgcgg aagaggctga aagggagacg
     3661 gaagacctta aaaaggttga gtttatgaag aagtatgagg gagaagaatt tgaaggaata
     3721 atatcaaaca tcacttcttt cggtatgttt gtagagctgg agaacactgt tgaaggactt
     3781 gtcagaatga gcagtatgga agatgactac tatgttttta acgaaaagaa ttatagcctg
     3841 ataggcgaaa aaagcagaaa ggtatacaga atcggagatg tcgtaagagt catgctggtt
     3901 agggctgata tcgcttcaag aaagatagaa tttgcggttg tggacaatga agacttgaag
     3961 gaaaaagaat ctgaaacaga ggagcaaacc gcacaggaaa caaagcgtaa gagaaagaga
     4021 aaggtagagg agtccgtgct gttgcatgtc aagggaaaga aaaaagtaaa aaagttaaaa
     4081 aagtaaaggg ggggatccgc aattatactt gtgagtgcat gccttcttgg ggtaaattgc
     4141 aaatataacg gtacgaacaa tttgaatagt gatgtgataa agcttctttc cgaggagaag
     4201 ttgatacctg tctgtccgga acaattgggc gggtgttcta cgccaagacc cccggtggag
     4261 ataatcggcg gtgatggaag agatgtattg gatggcaagg gcagggttat aagtaaaaac
     4321 ggggaagacg ttacacaaaa tttaatccgg ggagcgaatg aggtgttaaa gctcgcaaaa
     4381 acattgggca ttaagaaagc catactgaag gagagaagtc cgtcctgcgg tacctgcagc
     4441 atttatgacg gtacattttc aggtaaaacg ttaagaggaa gcggagttgc tgcacaaatt
     4501 ctcaagaaaa gcggtattac ggttatctct gaggaagatt tgaagaacgg ggagtgaaat
     4561 tcggttaaac aaaaaaggag gcaaatcgga tgtttacctc cttctattgc agttatagga
     4621 tatagttctt gatttcttcg cagaattctg agcagtcatc agaatgaaca tctactttga
     4681 tgggtttgct taagtcgagg ctgaatatac ccataatgga ttttgcatca accacataac
     4741 ggcctgatgt taaatcaatg tcgaaatcgt atttattaac tatattaacg aaatccttta
     4801 catcagtgat tgacttaagt gaaatattaa atgacttcat acagtttccc ccttttacgt
     4861 ttcttttcac gattttcttt catggttata tattaacctg tttgttgttg caagtcaatt
     4921 gtgatattgc taaaatttac ttagtatttt atatactttt tttaaataag ataagttgcc
     4981 aatgagtttt acaatttggt accggaggag tttggaatat ataatgaaaa gggttgcgtt
     5041 ttgtacactc ggatgcaagg taaatcaata tgagaccgaa gcactgtccg ggattttcag
     5101 aaaggccggc tataatgttg tagactttga agaggaggcc gaagtctatg tgataaatac
     5161 atgcacggtg acaaacctaa gtgacaggaa atccaggcag atgataagaa aggcaaaaaa
     5221 gatcaacaag gactctgtgg ttattgttgc cggatgctat gcccagaccg cacctgaaga
     5281 ggtaagcaag atagaaggcg taaacatggt tataggaacg aaagacagag gcagaattat
     5341 tgagtatatc gaagaattgg cggcaggtgg agacaagctc aatttcgttg atgatgtaat
     5401 gaaaacaagg gagtttgagg aactgggtgt tgatgtctac agggaacgta ctcgggcatt
     5461 tataaagata caggaaggat gcagccaatt ttgttcatac tgcattatac cttatgcccg
     5521 ggggccggta aggagcaggg cagaaaaaaa tattattgat gaaataaccg gccttgccag
     5581 taagggttac aaagaggtag tgctgacagg tatacacatt gcttcttatg gaaaggatat
     5641 taagacaact tctttagtgg atattgtaaa aaaagtccat gggattgaag gcatagaaag
     5701 aataaggttg gggtcaattg agcccacctt aattaatgag gagtttattg aatcaataaa
     5761 aaacctggaa aaggtttgtc cccaatacca tctttccctt caaagcggat gtgacagaac
     5821 attgaaacgt atgaacagga aatatactac agaagactac agaaaatccg tggcacttct
     5881 aagagaaaat attagggatg tagcaataac gacagatatc atggttgggt tcccggggga
     5941 aacagatgat gaatttgacg aaacatacag atttcttcag gagatttcct ttgcaggaat
     6001 gcatgttttt aagtattcgc caagaaaagg tactcctgcg gcgtcatacc cgaatcaggt
     6061 ttcaccgcaa aaaaaggagg agaggagtga tatgctcata aaccttgctt cgagtatgaa
     6121 gcacagcttc aacaagggtt atttaggaaa ggtgatgcct gtgttgtttg agcaggaggt
     6181 aaagggaatg gaaggatatg ttgaaggttt gactccaaat tacataagag ttgtttgtga
     6241 gggcagtgca ggatttgagg gaaacatcat gaaggtgctt ttaaaagagg ttgtttcgga
     6301 ttatatgacg ggagagacaa ttcacaaaaa tgagagttaa gaaggggaac aaaagctccg
     6361 ggagcaacga tctcccaacg ctttttggtc atttcacttc gtgaattcca ctaataaagg
     6421 tgaagaaaaa ttgctttctt cacaatactg gttacattta ttatacatac ccggataatg
     6481 tacacgattt taaggagttt gctttacaac gagattgagg gattatatag tagaaaattc
     6541 gaataatcaa ggcttgttta aaggtaaaat atattgcata aattatcctg ttgtattact
     6601 atatatatgt gggcttattt tcttgtagga atgatgagat gatggcaata gggtccttat
     6661 tttggacagg agagtgatta gttatggcag acaatgaaac aatgatgttt aaagtagaaa
     6721 aggaaaaggt taatgaggca agagaattac tcttatctgt ttatcaggcc ttgaaagaga
     6781 aaggctacaa tccgattaat cagattgttg gctatattct ttctggagat ccgacttata
     6841 taactaatca tatggatgcg agaagtgcaa tcaggaaatt ggaaagagat gaattattag
     6901 aggaaatact aaggttttat ttagaagaaa ctgcaaataa aggctccgta taatcgatac
     6961 ggagtttttt atggtgttaa cgctgtagtt aggttggtgt agacattgga tttatgtaaa
     7021 ttgggtaata caggtctgga agtttcaaaa ctgtgctttg gcggtcttat tatcgggccg
     7081 cttcaagtta atctctcgcc tgaacaaggt gcagatgtaa tacttaaagc cattgagctt
     7141 ggggttaact ttatagatac cgcagagctt tatggtacct atccacatat ccgggaagca
     7201 attaaaaaat caaataaaca aataataatt gcatcgaaat cctatgccta ttcaaaagaa
     7261 ggtgcaagag acagtcttga gaaggctaga agagaattgg atgttgatgt tatagatatc
     7321 tttttgcttc atgagcagga aagcaggttg acgcttaggg ggcatagaga agcattggaa
     7381 tattatattt caatgaaaga aaagggaatc atcaaagctg tcggagtatc aacccataac
     7441 attgaagtgg tggatgcctg tgcacatatg cctgaaattg acgttattca tccactgata
     7501 aacaagcatg gaataggcat tggagacgga acaatagatg ccatgctgga agctgtaaaa
     7561 aaagcttatt ctgccggaaa aggcatatat agtatgaaac ctttgggggg aggaaacctc
     7621 attaattctt ataaggaaag catggatttc gtgttaaata ttccctatat acattccata
     7681 gcccttggta tgcaatccat agaagaggtt ataatgaatg tatgtgtatt tgaaaacaaa
     7741 gctgtacctc aagaaatcca agaaagtctt tcaaaaagaa aaaggcgtct tcatatcgat
     7801 tggtggtgcg agggttgcgg aaaatgtgta cagcggtgca aacagggggc acttagcgtg
     7861 gttgataata aggcaagggt cagggaggat aaatgtgttt tatgcagtta ttgtgcctct
     7921 gtgtgtcctg tattcgctat aaaggtttgt tgatttgatt cagtaaggtt tttattaagg
     7981 tatatcggag gtagatatga ggatcatggg tattgattat ggcgacagca gaataggaat
     8041 tgcggtaagt gatcctatgg gatggacagc tcagggactt gaaacaataa agtggaaaac
     8101 ggatattgaa gttccactga agcgaatttc ccagttgatt aaagagtacg atattcaaaa
     8161 aatcattgtg ggttttccga aaaacatgga tggtactgtg ggctttcggg gtgaaaagac
     8221 cattgagttt atagatttat tgaagaaaag aacaaacaac atcgaagtta ttatgtggga
     8281 tgagaggctt tcaactgtag cggcaaatcg tacgatgcat gaaatgggtg tgaaaaaatc
     8341 aaaaaagaag atggtggtgg atcaaatcgc agcggtttat attttgcaag ggtacttgaa
     8401 cagtatagga aatacaacca tgtgattaat gggaaaccga aataaatatt ttaattgatt
     8461 tttgcgaaac atgatattga atgcaaaaga atgaaaatta gagcatatgc attcaatagc
     8521 tttaggaata tgtttcgcaa aaacagaaat aaatatttta attgattttt gcgaaacata
     8581 tagattatga tggaatttta taaaaactgt gatataattt aaatctataa tggaaaacag
     8641 gaggaatatt tttatgagtg aagaaagaga tgacttggtg gttttggttg atgaaaatgg
     8701 agaagaaacc gagtatgaat atctagatac aattgagttt aacgagaacg aatatgtggt
     8761 actcctttcg gttgaacaga gtaacaatga agataacatg gatgaggtag ttattttgaa
     8821 aattgttgag gatcctgatg gagaagacag ctttgttact attgatgatg aaagtgagat
     8881 ggaagcagtt tttgaagagt ttaagacaag aatggcggag gaatttgatt tttatgagga
     8941 atgaggcttg agtatatccc cgtaaaggct tagaaacaga ctgaagttgc gggggattaa
     9001 tttagagcgg ttttacagag taaattatgt gggaagccaa gggggcacct tgcccctgac
     9061 atttttaatc ctcattcgta agaattgaag gattttactt ttttatgtag aaatataacg
     9121 agacggtaaa tcttctctac ttatgaaagc ggcaggtaca gatttgcctt tcaggcggca
     9181 agtacatcat ccgcctcgtt gaaacactgc tgtggaaaga aatttttaca accagtaatt
     9241 tttctttgaa ccgggtagtt ataaatcttg attaacgaac aatatttaat gagggataaa
     9301 aatgtttaat gttacaatgt tgaaaaaaat gattttagtt gcgatgatat tcgtatcggt
     9361 aacattactg tcagcctgtg gcaaaagctc ggaaaccgat attgaagttg taagtccgtc
     9421 acctacccaa acaaaagttt atttcaccga aaagcccgaa tatactcctg agccggagca
     9481 aaaagtggat tttgatctac ccgaaaaagg aataagaccc tatgcggtaa tgatagataa
     9541 ccagggtaca agagttctcc cgcaaggcgg attgaacaaa gctcaggtga tttatgagat
     9601 aattgttgag ggaggaataa caaggttcat gcctgttttt tggggagagg ggtcaactat
     9661 gataggtcct gtcagaagct cccgccatta ttttcttgac tatgcaatgg aacacgatgc
     9721 aatttatgta catgaaggat ggagccctat ggcgaaggat gacatatcca aactgggcat
     9781 aaacaatata aactatgggg tattttggga tttgaccacg gataagagta attggcagga
     9841 tacctatacg tcgatggata aattaaagga atttactgaa aaagttaagt atcggaccca
     9901 gactgaaaaa gaactggttt ttaaatatgc cgacaaggat tatgagcttg aaaacggtag
     9961 caaggcagaa aaaattaagc tttcatattc ttgggattac atatccgaat acacctatga
    10021 tcctgaaaag aaattgtatc tcaggttccg gc
//
LOCUS       DAOOJC010000026         9917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_6081_length_9917_cov_3.763942, whole genome shotgun sequence.
ACCESSION   DAOOJC010000026 DAOOJC010000000
VERSION     DAOOJC010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9917
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6081_length_9917_cov_3.763942"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="PLA01_01440"
     CDS             <1..1200
                     /locus_tag="PLA01_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99994.1"
                     /translation="TGCDVLPPPAGAIKPPQYSNDVNHSGEDVKAIARRFLTPGTQFA
                     YPIHPKNEDAVKEIDIEGDGQREIIALYRGREINNNNNEVFGVFILKQGENQEWNVFW
                     QYQYDASITALDWGEVRDISGDGYPELLLGWSIGYRMGSNLDIFSLVDKPEPKLISSI
                     KYSEITDIEDRKGNNNEVRPIFVMYLYDKDMIESTDEGRRAELLKVLRWGQSDSTEQG
                     LIPAKDVYGVYYTDTIEEMKTSLSNYWNDFRRWYVLADAQIKCGKSDDALTSVENGLN
                     VVQSFINRRTYDKNSADDLLMYQRKFKILKTEAYIELGMYYEAIYELNKISDTYSGEN
                     EELVRVYLNLGRAYIGVNQFSNASLYLNKSCEMAELLYEKGSALYIINSYPAKVELQY
                     IKPFISK"
     gene            1359..2159
                     /locus_tag="PLA01_01445"
     CDS             1359..2159
                     /locus_tag="PLA01_01445"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPC99995.1"
                     /translation="MNLLNKLERKFGRFAIKGLPVYIVGLNMLVFLLTLIEVDFLQYL
                     FLVPSLVLKGEVWRLVTYIFIPPQISYSGIPWILFALYLYFIFASGLEHEWGSFKFNI
                     YYFIGMLGTTVAAFIIPIPATSSFIDLSIFLAFAYLYPDYEIRIFMIIPVKVKYLGWL
                     SWAYIIFQLIVYPWIGKLYVIIPILNFFLFFGKDIYIRYKTRGSSIYRKRKYREGYRE
                     KQYVHKCTVCGITEKDNPDMDFRYCLSCEGSYEYCMDHLKSHEHKKAE"
     gene            2569..2934
                     /locus_tag="PLA01_01450"
     CDS             2569..2934
                     /locus_tag="PLA01_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HPC99996.1"
                     /translation="MLQIKVYDAEYKFMNIVWKHSPIKSSKLVELAYEELGWKKSTTY
                     TVIRRLCDRGAIKNENSIVYALIEREQVMCSESEEHIDKFYDGSLKLFFSTFLRKEKL
                     TIDEINELKKIIKKHGGEI"
     gene            2936..5320
                     /locus_tag="PLA01_01455"
     CDS             2936..5320
                     /locus_tag="PLA01_01455"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HPC99997.1"
                     /translation="MLENMFMTIIKMSATASIAAVMIILLRLIVGRKLPKTFYYAAWA
                     IVLIRLLVPFSISTGFSVFNFIPSYSGEEHQQVNMHQYEEIKTIRNADVNEGVSVLHQ
                     DTTFNDNFDNPVNVLEDEKGINNTTGNIQSQNENDTKGSNIQELSATTAIAFIWLSIS
                     LVLLGFCIYAYLKTSKRLKTAVLFDDNGLVEECSSKLNFNRKTNVFTSERVDTPVVAG
                     IANVRIILPAFLVNKCDSKDLKYVITHELVHIKRHDNIIKLLAVLAVCIHWFNPLIWI
                     SFMLFQKDMEMSCDARVLSVYKNDVRKEYAESLLNIATKQNALMYSGLLGFGESNIKD
                     RIKGIMKFKNYGIWMKIFLVIILVILGFILLTNGKINDTDKDNTYSSNLQEVNGEDSV
                     NKNTTMNENDEFNELKIHITAMEELMVRKSNFQYMNEDQIEEILRTLPEIIRDNYRGV
                     GRIENNDSGYILLTCKSGAKRLPEDASTYERSFGSTKISQQIMEDSQGKEICRFPQGM
                     YVSYYTDKDILFIAVGDMSPNMDQIMQIEVFQLSGYQDYINYAKKAKERGSSIFNTGV
                     YPQIVTTICVDGIKVNEYVSVAEAFGEGMKEKLESHDETSCDTSTLIQTGIAILLGEE
                     DEKSYELCLLPDGTKVIHKYHEKDKVIINNGIYDSIMDIAGTRTAWEWVELYEIHDIV
                     QAEMRMKLGRDSEEQIQVVEDSQSLKELETLFSNAKYCGLTGCPFTASLYLTRKDGKV
                     ITLQIATDSCDTMILGSSAGYDYGPEPIGPGIEGTVNRQDVLKRIFHDIKWENQ"
     gene            5326..5790
                     /locus_tag="PLA01_01460"
     CDS             5326..5790
                     /locus_tag="PLA01_01460"
                     /inference="COORDINATES: protein motif:HMM:NF016932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HPC99998.1"
                     /translation="MEGCEDWRYSELMVCLPPDWPVSGEDFKDGKNYWPIWLLKMLAR
                     FPHKYKTWISLGHTIPNGDPAEPYADNTKLCCTLLLPPIDVNRDFLSLKVNDDVTVKF
                     LSIVPIYKEEMEYKLKYGVEALMDKFDKYNINEVIDINRGNVCRRSIWPFNR"
     gene            6160..6285
                     /locus_tag="PLA01_01465"
     CDS             6160..6285
                     /locus_tag="PLA01_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC99999.1"
                     /translation="MRCTLISILPRGLLIVGILLNLCLKIEEAIVTNRYKTVYLA"
     gene            6448..8046
                     /locus_tag="PLA01_01470"
     CDS             6448..8046
                     /locus_tag="PLA01_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HPD00001.1"
                     /translation="MKKLIRSLSLLLVVSILSVFMVACTIDEISLIDSLVKTSQITSY
                     ERNAKIELTFEGQSDSEEMQKAIDFIEAYVDEFSMSWNEKFSANEEMTKTKMAADFDV
                     DMKGLGVKMNYWMDVDFSGDNPQMKYIVELPPPITQPFFSSAGLESKKYIALDFAKNE
                     EIGADMDFNNVAKKSTELRDMVLDFIKTSATNFEPGMIAVNKKGSATTDRGEKVTEYE
                     LKLSDATAKQLLKSFINEVVLQEDSAEFIKKYLEATLEMIEMEEEDKQEALEELDKSI
                     NEMFIEQLPTYRENVAQVFEMINDVKFLGDKGVTINYFVNKDGYIVGTNASIDIEINL
                     ADLAAAVGSTDVGEGTFRFGITCESSLNNINKEVNVEMPELTKDNSLDILDMIETKTT
                     GPLFGVPSISDLTDYTNISEPVLYDGINVFMNGRHIAFDDTKPENINGRILVPVRTIS
                     EEMGADVSYNNETKQVTIVKGDKTIVLTIGSQEAYVNGEKVLFDVPAMIVEGRTMVPL
                     RFVSENMDAYVDWDGESQVVSIIY"
     gene            8224..>9917
                     /locus_tag="PLA01_01475"
     CDS             8224..>9917
                     /locus_tag="PLA01_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPD00002.1"
                     /translation="MWRRKSQKLTALVLAIGFIISSLTVTSYAEEEEKTVKPYCTRSS
                     SQAMAVNLGGGEDVTVDNTNFKKHSDFTNLMVEVKEPSESSKPNGTIDAFSIIQAENC
                     SENHGLVIEDCPDAGGTKNLAYIANGDYTAYENIYFPKGTKGFMARVSSDTEGGYIEL
                     RIDSTMGEVVGKCRVNNTGGWQNYTDVFCELTKSVEGVHTLYMGFVGDRDGLFNVNWF
                     RFTKSPYDPIIAKSYDESAEGAYLYKYVDFGEDSVPTKFKAYISSSTSGNISVRLDSP
                     DGYAIATVNTGSSKGYVECDVDESVTGIHELYLTDDKNGALVSKVNWFVFEPEEYFVN
                     LSDDNREKLAETGVRGYNVNLTASLDKNNVYEVYIYPSEFHKKNLQVFDIYINDTLVD
                     TVNTEESGLNLEKKGPYLTKVLDDGKLRIECRSKQGVVALSGLLINRITYSKLFKDVR
                     IKDWFFVPVMELASQGVIFGKGNDEFKPQDHIIGEHVAYMMFNVMKESVAEGDSSFKP
                     ERYRNLSDVPPSFWAYHYMNAYYRYFFTEKMLPYDVNTRVQYSASEFAESKKVRRE"
BASE COUNT         3322 a         1519 c         2199 g         2877 t
ORIGIN      
        1 actgggtgcg atgtattacc gcctcccgct ggtgctatca agcctcccca gtattcaaat
       61 gatgtcaacc acagtggaga ggatgtcaaa gctatagcaa gaagattttt gactcccggc
      121 acccagttcg cataccccat tcacccgaaa aatgaggatg cagtgaaaga aatcgacatt
      181 gagggtgatg ggcaaagaga gataatagct ttatatagag ggagagaaat aaataataac
      241 aacaatgaag tgtttggggt atttatttta aagcagggag agaatcaaga gtggaatgtg
      301 ttctggcaat atcaatacga tgccagtatt actgcactgg attggggaga ggtaagggat
      361 atatccggtg acggttaccc tgaattgttg ctgggatgga gcatcggata cagaatgggc
      421 agcaatctgg acatattttc tcttgtagac aagcccgagc caaagctgat aagcagtatc
      481 aaatattctg aaataacaga tattgaagac agaaaaggta ataataacga agtcagaccg
      541 atatttgtaa tgtatttata tgataaagac atgatcgaat ctactgatga gggaagaaga
      601 gcagaattgc ttaaagtcct cagatggggt caatctgatt ctacagaaca gggacttata
      661 cctgctaagg atgtttatgg agtatattat acggatacta ttgaagagat gaaaacgagt
      721 ttgtcaaatt actggaatga ttttcgtaga tggtacgtgc ttgccgatgc tcaaatcaaa
      781 tgcggaaaat ccgatgatgc actgacttct gttgaaaatg gcttaaatgt tgtgcaaagc
      841 tttattaaca ggcgtaccta cgataagaat tccgctgatg atcttcttat gtatcaaaga
      901 aagttcaaga ttctaaaaac ggaagcatat attgaactgg gtatgtatta tgaagcaata
      961 tacgaactaa ataaaatatc cgacacctat agcggggaaa acgaagagct ggtaagggtc
     1021 tacctgaatc ttggacgggc atatatcggg gttaaccaat tcagcaacgc aagtttatat
     1081 cttaacaaat catgtgaaat ggcagagctt ttgtatgaaa agggttcggc actgtatatt
     1141 attaattcat accctgcaaa agttgaattg cagtatatta aaccttttat atctaaatag
     1201 gaaaagagca ttattatttc tgaagatttc aaatacaccc ttggatttag tacctttttt
     1261 tgataaaatt aaaagagccg ctttttggtg ggatttctca aagtaaaagg actaactata
     1321 aggagtgtat tttttatatt aatccgagga gtggacgtat gaatttgctt aacaagctgg
     1381 aaagaaaatt cggaagattt gcaataaagg gattgccggt gtatatagtc ggcttaaaca
     1441 tgcttgtatt tctattgaca ctgattgagg tggatttttt acaatacctc tttctggttc
     1501 cttctcttgt tttaaaggga gaggtatgga ggttggtaac ctacattttc attccgccgc
     1561 agatatccta tagcggtata ccatggattt tgttcgctct atacttgtat tttatttttg
     1621 catcaggtct ggagcatgag tggggaagct ttaagttcaa catatactat tttatcggta
     1681 tgctgggaac cacagttgca gcgtttatta ttccaatacc tgctacgtct tcctttatag
     1741 atttatcaat ttttctggct tttgcgtatt tgtatcccga ctatgagatt cgtattttta
     1801 tgattattcc tgtcaaggta aaatacctgg gctggctttc atgggcatat attatttttc
     1861 agcttatagt atatccctgg attggaaagc tgtatgtgat tatacccata ctgaatttct
     1921 tcctgttctt cggaaaggat atttacataa ggtacaagac ccggggcagt tctatttaca
     1981 gaaagagaaa gtatcgggaa ggataccggg aaaagcagta tgttcacaaa tgcactgtat
     2041 gcggcattac ggaaaaagac aacccggata tggatttcag gtattgcctg agctgtgaag
     2101 gaagttacga gtactgtatg gatcacctaa agagtcatga gcataagaaa gctgaatagc
     2161 gaagtaaaat aggatgagtc agggaaaata acctggctca tttttgtgtg tgggtggatc
     2221 aataaagttt taggcatgaa ggacgctaat tacattatta ttccacaaac taagtttcga
     2281 gcctaaagag gctgtaaatt cggctcgggt tcgagcaaaa ctcgcttcgc tcaaacaatg
     2341 ctctcacttt cctcgcacga attaacagcc tctaatagtc tctccacaat gtttgttcca
     2401 taataatgta attagctgct ttaaggcagg ttatgtgttt aatgcgaaaa atagtttata
     2461 aatgcacaga atatggcaac tagaattaat acaaattgta tattgacaac tttggtctaa
     2521 tgtattaaac taagattagt ttaacgcatt agaccaaagg aggattttat gctacagatt
     2581 aaagtatatg atgccgaata caagttcatg aatattgtgt ggaagcattc gcctattaaa
     2641 agttccaaat tagttgagct tgcatatgaa gaactcgggt ggaaaaagtc taccacatat
     2701 actgtcataa gaagactttg tgacagagga gcaattaaaa atgaaaattc tatagtttat
     2761 gcattgattg agcgtgaaca ggttatgtgc tctgaaagcg aagaacatat cgacaagttc
     2821 tatgacggtt ccctgaagct ttttttctct acatttctta ggaaggagaa acttaccatt
     2881 gatgaaataa atgaattgaa gaagataata aaaaaacatg gaggagaaat ataatatgct
     2941 tgaaaacatg ttcatgacaa taattaagat gagtgccaca gcttcaatag ctgctgttat
     3001 gataatatta ttaaggctaa tagtgggaag aaaacttcct aaaacctttt attatgctgc
     3061 atgggcaata gtactgatac ggctccttgt cccgttttca atttcaactg gcttcagcgt
     3121 attcaatttt ataccgtcat atagtggtga agagcatcaa caagtaaata tgcatcaata
     3181 tgaggaaata aaaacaatcc ggaatgcaga cgtcaatgaa ggagtaagtg tacttcacca
     3241 ggatacaacc tttaatgata actttgacaa tccggttaat gttctagaag atgaaaaggg
     3301 tattaataat actactggta atatacaatc acaaaatgaa aacgatacaa agggcagtaa
     3361 tattcaggaa cttagtgcaa ctactgcaat tgctttcata tggttgtcga tttccttggt
     3421 attgctgggt ttttgcatat atgcatattt gaaaacgtct aaaaggctaa agacagcagt
     3481 tctttttgat gataacggat tggttgaaga atgcagcagc aaactaaatt ttaaccgcaa
     3541 aacaaatgtt ttcacttctg aaagagtaga tacacctgtt gtagccggta ttgcaaatgt
     3601 tcggataata cttcctgctt ttcttgttaa taaatgtgac agtaaggatt taaaatacgt
     3661 gattacccat gaactggttc acataaagcg acatgacaat attatcaagc tattggctgt
     3721 tcttgctgta tgcattcatt ggttcaatcc gttaatatgg ataagcttta tgctattcca
     3781 gaaggatatg gagatgtcct gtgatgccag ggttttgtcg gtatataaaa atgatgtacg
     3841 aaaggaatat gcagaatctc ttttgaacat tgcaacaaaa cagaatgcac tgatgtatag
     3901 cggacttctt ggattcggag aaagtaatat caaggacaga attaaaggaa taatgaaatt
     3961 taaaaactat gggatctgga tgaaaatttt tttggttatt atccttgtta ttcttggctt
     4021 tatactttta acaaacggga agattaatga tactgacaaa gataatactt attcgagtaa
     4081 tctgcaggag gtcaacggag aggatagcgt caataaaaac actaccatga atgaaaatga
     4141 tgaatttaat gaactcaaaa tacatattac tgccatggaa gaactcatgg taagaaaaag
     4201 caattttcaa tatatgaatg aagatcaaat agaggaaatt ctaaggacct tacctgaaat
     4261 tattcgggac aattatcgtg gtgttgggcg tatagaaaac aacgacagcg gatatattct
     4321 gcttacctgc aagagtggtg caaaaaggtt acctgaagac gcaagtactt atgagagaag
     4381 cttcggcagc acgaaaataa gccaacagat catggaagat tctcaaggga aggaaatttg
     4441 ccgttttccc caaggtatgt atgttagtta ttacacggat aaagatattt tatttatagc
     4501 agtgggagac atgtctccaa atatggatca aattatgcag attgaagtat ttcagctttc
     4561 cggatatcaa gattacataa attatgcaaa gaaagcaaag gagaggggaa gttccatatt
     4621 taataccggg gtatatcccc aaattgtaac aaccatatgc gttgatggta tcaaggtgaa
     4681 tgagtatgta tctgttgcag aagcttttgg ggaaggtatg aaagaaaagc ttgaaagcca
     4741 tgatgaaaca agctgcgata ccagtactct tatccaaacc gggatagcaa ttcttttagg
     4801 agaagaagat gaaaaatcct atgaattatg ccttctgccg gatgggacaa aggttatcca
     4861 caaatatcat gaaaaggata aagtaattat aaacaatgga atatatgaca gcattatgga
     4921 tatagccgga accaggacag catgggagtg ggttgaactg tatgaaatac atgacatagt
     4981 tcaagccgag atgaggatga aattaggaag ggattcggaa gaacagatac aggttgttga
     5041 agattctcaa agcctgaaag agttggagac tctgttttca aatgccaagt attgtggctt
     5101 aacgggctgt ccctttaccg cttcccttta tctcacccgc aaggatggaa aggtaattac
     5161 attgcagata gctactgata gttgcgatac aatgattctc ggctcttctg ccggttacga
     5221 ttacgggccc gaaccaatag gtcccggtat agaaggaact gttaaccgcc aggatgtact
     5281 caaaaggatt tttcatgata taaaatggga gaatcagtaa agattatgga ggggtgtgag
     5341 gattggagat actcggaact tatggtatgt ttaccgcctg attggcctgt tagtggagag
     5401 gattttaaag atggaaaaaa ttattggccc atttggttat tgaagatgtt agccaggttt
     5461 ccgcataaat acaagacatg gatatcgttg ggacatacta ttccgaatgg ggatccagca
     5521 gaaccttatg cagacaacac aaaactatgt tgtacacttc ttttgccacc aattgatgtc
     5581 aatagggatt ttttatcttt gaaagtaaat gatgatgtaa cagttaagtt cttaagtata
     5641 gttcccattt acaaagaaga aatggaatat aaattgaagt atggtgttga agcattgatg
     5701 gataagtttg ataaatacaa tataaatgaa gtgattgata ttaatagagg aaatgtttgt
     5761 agacggtcaa tttggccatt caacaggtaa gaccatatag tgcagtgtgc caatgatgaa
     5821 agtcaaacga gttgagagtc taatattgta actaaatcat gcattgcctg agctgtgaag
     5881 gaagttacga gtactgcatg gatcacctga agagtcatga gcataaggaa gctgaatagt
     5941 ggagttaata tagatgggtc aggaaaatta acctggctca ttttttatca aggttgaagc
     6001 ttcataagca aataatgaat aaaattttaa aagaaaatga gaataaatta accgaaacgt
     6061 gagatacctt ataattcgta tgttaaaatt ataatatctt catttttccc ataattgaat
     6121 cgtaacatgt gatgtatcaa tgagttcggg atggaaaaca tgaggtgtac gttaatttcc
     6181 attcttccca gggggcttct aattgttgga atcctcctta atttatgtct gaaaatagaa
     6241 gaggctattg taaccaaccg gtataagacg gtttaccttg cttaacactt tcaaaaaggt
     6301 ttatttgaat tacttaattt agataaattt ttaaaaagac aacacattct tgataattgt
     6361 gttataatgt aaaagtattg gaataaacta ttccataaaa aactaaacgt acaacactat
     6421 aagattatta ttagggggtt atgaaacatg aaaaagctaa tacgaagcct atctttattg
     6481 ctggttgtat caattttgtc ggtatttatg gtggcatgta ccatcgacga gatttcacta
     6541 atagactctt tagtgaaaac atctcaaata acgtcctatg aaagaaacgc aaaaattgaa
     6601 ttaacctttg aagggcaaag cgattctgaa gaaatgcaga aggctattga ttttattgag
     6661 gcatatgttg atgagttttc gatgtcttgg aatgaaaaat tctcagccaa tgaagaaatg
     6721 acaaaaacca aaatggctgc cgacttcgat gtggatatga aggggctggg agttaagatg
     6781 aattactgga tggatgtaga tttttccggt gataatcctc aaatgaagta tatagttgaa
     6841 cttccaccac caattactca gccgtttttc tcgagtgccg gtttggagtc aaagaagtat
     6901 attgctttgg actttgcaaa gaatgaagaa ataggagctg atatggattt caataatgtt
     6961 gccaagaaat ccacagaatt acgggacatg gtacttgatt ttattaaaac aagtgcaaca
     7021 aacttcgagc ccggaatgat agctgtcaac aagaaaggtt cagcaactac cgatagaggt
     7081 gaaaaggtta ctgaatatga attgaagtta agtgatgcta cagcaaaaca gcttcttaag
     7141 tctttcataa atgaagttgt gctgcaggag gactctgcag agtttataaa aaaatatttg
     7201 gaagccactt tggaaatgat tgaaatggaa gaagaagata agcaagaagc actggaagag
     7261 ttggataaat caataaatga gatgtttatt gaacagcttc ctacatatag agaaaatgtg
     7321 gcacaagtat tcgaaatgat aaatgatgtt aaattcttag gtgataaagg agttacaatt
     7381 aattactttg tcaataagga cggttatatt gtagggacaa atgcatccat agatattgaa
     7441 attaacctgg cagacttagc agctgcagtt ggttcaactg atgtagggga aggcacattc
     7501 cgtttcggaa taacttgcga atcttcactt aacaatataa acaaagaagt aaatgttgaa
     7561 atgccggaac ttacaaaaga caattccctt gatatattgg atatgattga gacaaagaca
     7621 accggaccgt tatttggagt gccttcaata agtgacctga ctgattacac aaacatttcc
     7681 gagcctgtgc tatacgacgg aatcaatgtg tttatgaacg gaagacatat tgcttttgat
     7741 gatacaaagc cggaaaatat taatggaaga atactggttc ccgtaagaac aatatctgag
     7801 gaaatgggtg cggatgtgag ctataataat gaaacaaagc aggttaccat cgttaagggt
     7861 gataaaacaa ttgtattgac tataggttct caagaagcat acgttaacgg tgaaaaggtg
     7921 ctgtttgatg tacctgcaat gattgttgaa ggccgcacaa tggttccttt gagattcgtg
     7981 tctgaaaaca tggatgcata tgttgattgg gatggagaat cccaggttgt atctattatt
     8041 tactgattga cgtaccagat ggaggaaaag tctgatcagg ctttacctgt aacgaaatgg
     8101 aaggattgct gctttctata gaaagtggca tccttctttt tataatatac ttttaaatta
     8161 ttataaagtt tggtaaaatg taaaagcagt agcaagacaa aaaaagagaa aggggagttt
     8221 tatatgtgga ggagaaagtc acaaaaactt actgctttag tattggcaat tggcttcatc
     8281 ataagcagtt taacggtgac ttcgtatgca gaagaggagg agaaaactgt taagccatac
     8341 tgtacgcgat cttcttctca ggctatggcg gtaaatctag gtggcggtga ggacgtcact
     8401 gttgacaata ccaatttcaa aaagcattcg gattttacaa acctcatggt tgaagtcaaa
     8461 gaaccgtccg aatcttccaa gcccaatgga acaattgatg ccttttcaat aattcaggct
     8521 gaaaactgca gcgaaaacca tggactggta attgaagact gtccggatgc cggcggtaca
     8581 aaaaacctgg catatattgc aaatggagat tatacggctt atgagaacat ttattttccc
     8641 aagggaacaa aaggctttat ggcacgggtt tccagcgata ctgagggagg atatatcgag
     8701 cttcgtattg attctaccat gggagaagta gtgggaaagt gccgtgtaaa caatacagga
     8761 ggatggcaga attatactga tgtgttttgt gagctgacaa aaagtgttga aggtgtacat
     8821 actttatata tgggatttgt cggagacagg gacggccttt tcaatgtcaa ctggttcaga
     8881 ttcacaaaga gcccttacga tccaataatt gcaaaaagct atgatgagtc ggcggaagga
     8941 gcatatttgt ataaatacgt ggattttggg gaagacagtg ttccgacgaa gtttaaagca
     9001 tatatttcca gcagtacttc cggtaacatt agtgtaagac ttgacagccc tgatggctac
     9061 gctatagcta cagttaatac gggtagcagc aaaggttatg ttgagtgtga tgtagacgaa
     9121 tccgttacag gaattcatga actgtatttg accgatgaca aaaacggtgc tctggtttcg
     9181 aaggtaaact ggtttgtgtt tgaaccggag gaatatttcg ttaatttatc ggatgataac
     9241 cgtgaaaaac ttgcagaaac cggcgtaaga ggttacaatg ttaatcttac tgcttcattg
     9301 gataaaaaca atgtatacga ggtttatata tacccttcgg agttccacaa aaagaacttg
     9361 caggtgtttg acatctatat aaacgatact ctggtggata ccgtcaacac cgaggaatcg
     9421 ggcctgaatc tggagaagaa aggtccgtat cttacaaagg ttttggacga tggaaagctt
     9481 cgtattgaat gcaggtcaaa gcagggagtt gtagcccttt cgggtcttct gataaacaga
     9541 atcacgtatt ccaaattatt taaggacgta agaattaaag actggttttt tgttccggtt
     9601 atggagcttg caagccaggg agttatattc ggaaaaggta atgacgaatt taagccgcaa
     9661 gaccatataa ttggcgagca tgttgcatat atgatgttta acgttatgaa ggagtctgtc
     9721 gcagaaggcg acagcagctt taaacctgaa aggtatagaa acctgtcgga tgttccgccg
     9781 agtttctggg cataccacta tatgaatgct tattacaggt atttctttac tgaaaaaatg
     9841 ctgccttacg atgttaatac ccgtgttcaa tacagtgcaa gtgagtttgc ggaaagcaag
     9901 aaagtaaggc gtgagga
//
LOCUS       DAOOJC010000027         9827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_6156_length_9827_cov_3.191772, whole genome shotgun sequence.
ACCESSION   DAOOJC010000027 DAOOJC010000000
VERSION     DAOOJC010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 9827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9827
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6156_length_9827_cov_3.191772"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /locus_tag="PLA01_01480"
     CDS             complement(<1..1548)
                     /locus_tag="PLA01_01480"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HPD00003.1"
                     /translation="MDMSQYLEIFIDETKEHLQGLNQSLLQLEQNPQDKSVLNEIFRV
                     AHTLKGMSGTMGFNKMAKLTHDMENVLQALRNDQISVTSKLVDLLFKCLDALESYTEN
                     IVNSGEEGGNDYNDIITELNNSIEGKPSQATQAVKPVAVKREVKTEQQMYPTSNFKLN
                     QYEQNVVNKAIGMDMNAYKITVVLNKGCVLKSARSFIIFQTLERYGEIIKSVPTVEDI
                     EDEKFDFEFTVVCITKEAPEVFDKSINGIAEVDKVIVEPLEAMDLKNCPQSTEMPSSE
                     DIQSEDTENPQHEPEKKQEDNKGQSAQMHKAKTGKTVRVDIDRLDVLMNLVSELIIIK
                     TRLEGLEIKDSSQTYGEAIEYLERITTSLHDAVMKVRMVPVEMVFNRFPRMIRDVSRE
                     LGKEIQLNMSGEETELDRTVIDEIGDPLIHLLRNSADHGIESKGKRVQAGKPVVGQIN
                     LKAYQDGNNVVIEVDDDGQGIDLEKVKKKAIERGLVKKDMANTLSQKDIVDFLFKPSF
                     STSDNISN"
     gene            complement(1548..2618)
                     /locus_tag="PLA01_01485"
     CDS             complement(1548..2618)
                     /locus_tag="PLA01_01485"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HPD00004.1"
                     /translation="MTKSRIPSKIRVLVVDDSAFMRKAIMDILNSDDEISVIDTAKNG
                     VEAIEKAQQLKPDVITMDLNMPVMDGLECLRRLQAIIRIPVIMLSSYTKEGASATMQA
                     LDEGAMDFVAKPGNIFEIKRDDMRAEIIRKVKICKNLRTPEVGEINTEPVEKTSNMYG
                     NKIKSIVAIGTSTGGPKALQEVIPLISPDVESAFLVVQHMPAGFTKSLAERLDSMSGV
                     NVKEAEDNEVVKNAHVYIAPGDYHMTIRKTGDSNYRIKLSKDPPVEGHRPSVDVMMES
                     VADSGINNIVAVIMTGMGKDGTRGIEKLKNKNNVHVIAQDESSCVVFGMPRVAISAGL
                     VDAVVPLNQIAKEIMKTVGVHR"
     gene            complement(2628..3311)
                     /locus_tag="PLA01_01490"
     CDS             complement(2628..3311)
                     /locus_tag="PLA01_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPD00005.1"
                     /translation="MRINELKIGLKLEVRLYSPRDIRTDTLLVSEFEWAENDKIFFIA
                     VPIHRGRLYPIHIGTQMEVIFIMHDNLYSFIAKVLDRCIKNRISLLKMEALTQIRKIQ
                     RREFFRFECALPIGYRLVETQEHGDSENSDKEFIKTYTRDLSGGGVCIRLKEEVDYGA
                     IIECELLLNDINRIRFIGRVVRFTTYDSPVGMYKYEIGVFFEKISEKDRERVVSHIFQ
                     EQRRLIKKG"
     gene            complement(3353..4261)
                     /locus_tag="PLA01_01495"
     CDS             complement(3353..4261)
                     /locus_tag="PLA01_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HPD00006.1"
                     /translation="MMDQAEKLRKIIDNLKERHVAGQTGTLSIVDKTAKVITVTSGKG
                     GVGKTNITINLAIALSEMGKRVAILDADFGLANIDVMLGIIPKLTLVDVIYNNRNILE
                     VLTDGPKNIKFISGGSGVEELVKLDKEQLKRFIANISLLDKLADVVLIDTGAGISESV
                     MSFVMAANEVILVTTPEPTSITDAYALIKAVSNRDRNKNIKVIVNRAENANEAKDILT
                     KLSVVAGKFLTIDLHPLGFIPQDEQVIKAIKMQQPFILSYPKSQASKQIIEMSRKLIE
                     SNNEETASKEKGIRSFVQRLVNLMGG"
     gene            complement(4245..5567)
                     /gene="flhF"
                     /locus_tag="PLA01_01500"
     CDS             complement(4245..5567)
                     /gene="flhF"
                     /locus_tag="PLA01_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HPD00007.1"
                     /translation="MKIRRYIGKDTQEAILKVKMDLGNEALILNTRKVRQKGILKMFS
                     KPLIEILAAVDENYGTDNKKVAVKNEIGVDKRNEIGVYKRNEIGLDKRNEIDTDKKNE
                     IGVDKKNEVRTINGVGNDVFCKSKLNEKEEKIVLLENKINGMEAVLNKIYKQIQQHAE
                     KTEAVLDRDNDKSLSGILDLFYNNLVKNEVETDIVQYLIDRVHENKKENTGTDDTTSL
                     MYNLISDLLGKPSTIKLREDGKPTVIVLVGPTGVGKTTTLAKMAAHYALDHKKNVGLI
                     TADTYRIAAVEQLKTYAEILGMPLKVVYSADEISEAMEGYKDKDIVFIDTAGRSYKNK
                     PQFEELKTFVEASCADEVFLVLSTTTGIKNCKEIIENYDFLPNYKLIFTKLDESSSPG
                     VILNSRYRTNKELSFVTMGQSVPDDIEVANVDKITKNLLGAMAYDGPS"
     gene            complement(5568..7598)
                     /gene="flhA"
                     /locus_tag="PLA01_01505"
     CDS             complement(5568..7598)
                     /gene="flhA"
                     /locus_tag="PLA01_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HPD00008.1"
                     /translation="MRVKFGEISIAVIVVSIVLVIILPVPSGILDVLLALNIALAIVI
                     LLNVVYSKEAIQLSVFPSVLLFTTLYRLALNIKSTTLILANGEAGQVIEGFGNFVAQG
                     NLVVGAVIFLIITIVQFLVITKGTERVSEVAARFTLDAMPGKQMAIDADLNAGLINEM
                     EAKERRRKIQRESDFYGAMDGATKFVKGDAIAGLIITIINVIGGVIIGAVMRNEEISY
                     AFQNYTILTIGDGLVSQIPALIISVATGLIVTKSTSDEGLSVDLKKQILYNSNVFFIA
                     AGFCILLAIPLATLPFLTLAAVFIAIGIILIRKNAESEKQEGIQIEENEVKEIRKPEN
                     VVNLLQVDPIELEFGYGIIPLADVNQGGDLLDRVVMIRRQLALELGMIVPIIRLRDNI
                     QLSPNEYIIKIKGVEVGGGELMLDHYLAMSPGVVEEEIDGIKTTEPAFGLPAVWISEA
                     QRDRAEMLGYTVVDSPSIIATHLTEVIKSHAHELTGRQEVQTIIDKVKENYPAIVEEL
                     VPKMMTIGEIQKVIANLLKEGVSVRDMVTILETLADYAPSTHDTDLLTEYVRQALGRA
                     ISKRFIKDKKSNVITLDPKLEQVIMDSVQKTEQGSYLTLEPGIINTIINNLSKQVQKL
                     MQLGQQPIVLSSPVVRLYFRRLTEQSIPGLVVLSYNELDPGVEVQAVGTVSV"
     gene            complement(7674..8825)
                     /gene="flhB"
                     /locus_tag="PLA01_01510"
     CDS             complement(7674..8825)
                     /gene="flhB"
                     /locus_tag="PLA01_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HPD00009.1"
                     /translation="MDDKCQTAAGGDRGSLCAGGEKLKVNLQLFADSGEKTEKATPRK
                     KSKARKEGQVLQSREMTAAIVLLCMFIGLRAFGGFMYEEILGCFKKVFTVYFEEDNLF
                     TIDGVMKMFDETLITMLKIVAPLFAIALLTGLVSGFAQVGFLFSTKTLGVKLGKINPL
                     NGFKRIFSARSLVDLAKSIIKIAVVGYVGYSYIKGESKDMLSTMNMDVISIASYIGVT
                     ATNVGIRMGIAMLFIGVIDYGYQWWEYEKSLRMTKQEVKEEYKETEGNPQTKSRIRQK
                     QRQMSMRRMLQDIPNADVVITNPTHFAVAIKYDSKVADAPVVLAKGQDYIALRIKEIA
                     KENNVEIVENKPLARTLYETVEIGGKIPPELYQAVAEVLAYVYGLKRNR"
     gene            complement(8867..9652)
                     /gene="fliR"
                     /locus_tag="PLA01_01515"
     CDS             complement(8867..9652)
                     /gene="fliR"
                     /locus_tag="PLA01_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HPD00010.1"
                     /translation="MAIPEGILYSGIEIFLLIFVRMTGLFVIAPIFGGRNIPAYLKIG
                     FSFMLALILANTIKVQELVFSNIYEYFILVLKEFLVGITLGYVSYLIFTAIYLAGHII
                     DMQIGFGVVNIIDPMSNIQVPITSNFYFILCMLIFLMCRGHHILIRALFKSYNYIPLG
                     TGVFGKEIVNDILRVFGNIFFIAFRISAPILAAIIITDVALGVLSKTVPQLNVFMVGM
                     PLKITLGLAVMVLTIPVFISLMESLIRGMDGEMYTFLKDMAPK"
     gene            complement(9683..9823)
                     /locus_tag="PLA01_01520"
     CDS             complement(9683..9823)
                     /locus_tag="PLA01_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HPD00011.1"
                     /translation="MQEQTLTFIPKILAILSGIVIFGAWMLRVLIDFTQNIYLNINQY
                     IR"
BASE COUNT         2668 a         2373 c         1503 g         3283 t
ORIGIN      
        1 gttcgaaata ttatccgatg tgctgaaaga cggtttaaac aggaaatcaa caatatcctt
       61 ctgggacaga gtattagcca tatccttctt tactagtcct ctctcaatag cctttttctt
      121 aactttttcc aggtctattc cctgaccgtc gtcatctact tctataacca cgttatttcc
      181 gtcttgatat gcctttaagt ttatctgacc gacaaccggt ttccccgcct gcaccctctt
      241 tcccttggat tctattccgt gatcggcaga atttcttaaa aggtgtatga ggggatcccc
      301 tatctcgtca ataaccgttc tgtcaagttc agtttcctca ccggacatat tcagttgaat
      361 ctcttttccc agctctcttg aaacatccct tatcatcctt ggaaatctat tgaataccat
      421 ctccacaggt accatcctga ccttcataac ggcatcgtga agactggttg taatgcgctc
      481 taagtactca attgcttccc catacgtttg agaactatct ttaatttcca gcccctccaa
      541 acgggtctta ataataatca gctcactcac aagattcatc agaacgtcca gtctgtcaat
      601 atcgactctg actgtttttc ctgtctttgc cttgtgcatc tgtgcacttt gtcccttatt
      661 atcctcttgt tttttctcag gctcatgttg gggattttca gtatcctcag attggatgtc
      721 ttcagaagaa ggcatttctg ttgactgagg acagtttttt agatccatag cttccaacgg
      781 ctcaacaata actttgtcaa cctcagctat tccattgatg ctcttgtcga aaacttcagg
      841 agcctcttta gtaatacata ccaccgtaaa ttcgaaatca aacttttcat cctcgatgtc
      901 ttctaccgtc ggtaccgatt tgattatttc accgtatctt tcaagagtct gaaagattat
      961 aaaggagcgg gcagacttta aaacacagcc tttatttaat acgactgtta ttttataagc
     1021 attcatgtcc atcccaattg ctttattgac aacgttctgc tcatattgat tgagcttgaa
     1081 attcgaagta ggatacattt gctgctctgt ttttacttct ctcttaacag caaccggttt
     1141 aacagcttgt gtagcttgcg aaggtttgcc ttcaatactg ttatttaact ctgtgattat
     1201 atcattataa tcgtttccgc cttcttcacc gctgtttaca atattttcag tataactctc
     1261 aagtgcatca agacatttga agagtaagtc aacaagcttt gatgttacac ttatttgatc
     1321 gtttcttagt gcctgtaaaa cattttccat gtcatgggta agtttagcca ttttattaaa
     1381 acccatggta ccggacatgc cttttaacgt atgagccacc ctgaatattt cgttaagaac
     1441 tgatttgtct tgcggatttt gctcaagttg aagtaaagac tggttcagcc cctgcaagtg
     1501 ctctttcgtt tcatcgataa atatttcgag gtattgactc atatccatca tctatgcacc
     1561 cccacagttt tcataatctc cttcgcaatc tgatttaacg gaacaactgc atctaccagt
     1621 cctgctgaaa ttgccaccct cggcattcca aataccacgc atgagctttc atcctgtgca
     1681 attacatgga cattgttttt gtttttcagc ttctctatcc ctctggttcc gtctttaccc
     1741 attccggtca taattactgc aacaatatta ttgataccgc tatcggccac cgattccatc
     1801 attacatcaa ctgaaggtct gtgtccttca acgggaggat cctttgaaag cttaatccta
     1861 taattgctat ccccggtttt tctaatagtc atgtggtaat cccccggtgc aatataaaca
     1921 tgggcatttt ttacaacctc gttatcttca gcctctttga cattaacacc actcatagaa
     1981 tccagtcttt ctgcaagaga ctttgtaaat ccggcaggca tatgctgtac taccaaaaaa
     2041 gctgactcaa catccgggga tatcaacggt ataacctcct gcaatgcttt cggtccaccg
     2101 gtggaagtgc ctatggctac tatgcttttg attttatttc catacatatt acttgtcttt
     2161 tccacaggct ctgtgtttat ttcaccaacc tcgggagttc ttagattttt acaaatctta
     2221 accttcctga ttatttcagc cctcatatca tctcttttta tttcaaatat attcccgggc
     2281 tttgcaacaa agtccattgc tccctcgtcc aacgcctgca tggtggcaga agcaccttcc
     2341 tttgtataac tgctcaacat gatcacagga attctaataa ttgcctgtaa tcttcttaag
     2401 cattcaaggc catccataac aggcatattc agatccatgg taataacatc aggcttaagt
     2461 tgctgagcct tctcaatcgc ttcaaccccg ttttttgcag tatcaataac ggagatttca
     2521 tcatcactgt tcaaaatatc cataattgcc tttctcataa aggctgagtc atcaacaacc
     2581 aataccctta ttttacttgg aattctactt ttagtcataa accagtatca acccttttta
     2641 ataagtcttc tctgttcttg aaatatatgg cttactactc tttccctgtc cttttcacta
     2701 atcttttcaa agaatactcc aatctcatat ttgtacatgc caacaggtga atcatatgtt
     2761 gtaaacctta ccactcttcc aatgaaacgt attctattaa tatcatttaa taataattcg
     2821 cattcaataa tggctccata atcaacctct tcctttaggc gtatgcacac cccgcctccg
     2881 cttaagtccc tcgtataggt ctttatgaat tctttatcgc tattttcgga gtcaccatgt
     2941 tcctgagtct ctaccagtct gtagcctatg ggaagtgcac actcaaacct gaaaaattcc
     3001 cttctctgaa ttttccgtat ctgtgtcaat gcctccatct ttaacagact gattctgttt
     3061 ttaatacatc tgtcaaggac ctttgcaata aagctataaa gattatcatg cataataaat
     3121 atgacttcca tctgtgttcc tatgtgtata gggtataacc ttcccctgtg tataggaact
     3181 gcaataaaga aaattttgtc attctcagcc cactcaaact cactaaccaa aagggtatct
     3241 gtccggatat cccttgggct gtacaatcgc acctcgagct tcaaacctat ttttaattca
     3301 ttaatcctca tagcaacctc cccaccggtc cgtcttgact taacctactt aatcagccac
     3361 ccatcaggtt tacaagtctt tgcacaaagc ttcttatacc cttttctttg gaagctgtct
     3421 cttcattgtt actttctata agttttcttg acatctcgat aatctgcttt gaagcttgac
     3481 tctttggata actcaagata aaaggctgct gcatttttat tgctttaatt acctgttcgt
     3541 cctgaggaat aaaccctaat ggatgtaaat ctattgtaag aaatttccct gccacaacag
     3601 acagcttggt tagtatatcc tttgcttcat tggcattttc cgccctgttc actataacct
     3661 ttatgttctt gttcctgtcc ctgttggaca ccgcttttat taatgcatac gcatcggtga
     3721 tagaagtagg ttccggagta gtcaccagaa ttacctcatt tgctgccata acaaagctca
     3781 taacactttc ggagatccca gccccggtat caattaatac aacatccgcc agcttatcca
     3841 gcaaagaaat gttggctata aaccttttca gctgttcctt atcaagcttt accagttctt
     3901 caacccctga tcctccggat ataaacttga tgttttttgg tccatcggta agtacctcaa
     3961 gtatatttct gttattatat ataacatcaa ccaaagtaag ctttggtatt attcccagca
     4021 taacatcaat gtttgccagc ccaaaatccg catccaatat agcaacccgc tttcccatct
     4081 cacttaaagc tatagcaaga tttatggtaa tattagtctt cccaacgcca cctttgccgc
     4141 tggtaactgt aattaccttt gccgttttat caactatgct taaggtgccg gtctgtcctg
     4201 caacatgcct ctctttaaga ttatctatga tttttctgag tttttcagct tggtccatca
     4261 tacgccattg ctcctaataa attttttgta atcttatcaa cattggcaac ttcgatatcg
     4321 tcaggcacgc tctgtcccat tgtaacaaaa gacagttctt tattcgtccg gtaccttgag
     4381 tttaaaataa ctcctggcga agaactttca tcaagctttg taaaaatcaa tttataattt
     4441 ggcaaaaaat catagttttc aattatttcc ttgcaatttt tgatccctgt agtagtactt
     4501 agcacaagaa aaacttcatc ggcacatgat gcctcaacaa aggtttttag ctcctcaaat
     4561 tggggtttgt ttttgtagct ccttcctgct gtatcgataa atactatatc cttatccttg
     4621 taaccctcca ttgcctcact aatttcatcg gcagaatata ctacctttag aggcatcccg
     4681 agaatttcgg cataagtctt taattgttcc accgccgcaa tacggtaagt atcagctgtt
     4741 atcagaccaa cattcttttt atggtcaagg gcatagtggg cagccatttt tgccaaggtt
     4801 gtcgtttttc ctaccccggt aggacctacc agtactataa ccgttggctt tccatcttcc
     4861 cggagcttga tagtggaagg tttacccagt aaatcggaaa tcaaattgta cattaatgag
     4921 gtagtatcgt cagtccctgt attttccttc ttattttcat gaaccctatc aataaggtat
     4981 tgaactatat ccgtttccac ctcatttttt acaaggttgt tataaaacaa gtcaagaata
     5041 cccgataatg acttatcatt atctctgtct aggacagcct ctgttttttc ggcatgctgt
     5101 tggatttgtt tataaatctt attgagtact gcttccattc cgtttatttt attctctaaa
     5161 aggactattt tttcctcctt ttcgttcaat ttacttttac agaatacatc atttccaacg
     5221 ccgtttattg ttctcacttc atttttctta tcaacaccta tttcgttttt cttatccgta
     5281 tctatttcat ttctcttatc taaacctatt tcgtttctct tatacacacc tatctcattt
     5341 ctcttatcca cacctatttc attttttacc gcaacctttt tattgtccgt tccataattc
     5401 tcatcaacag cagccaaaat ctctatcaga ggttttgaaa acattttcag gatgcctttt
     5461 tgcctgacct ttcttgtgtt aagtatcaag gcttcgtttc ccagatccat tttaactttt
     5521 aatattgctt cctgtgtgtc ttttccaata taccttctaa tcttcattta tacactcacc
     5581 gtccccacag cttgaacctc aactccagga tcaagttcat tatatgacaa gacaaccaat
     5641 ccaggtatcg attgctctgt taatcttcta aaatacagcc ttacaaccgg tgacgacagc
     5701 actatgggct gctgacccag ctgcatcaat ttttgtacct gctttgacag gttgtttatt
     5761 atggtattaa ttattcccgg ctccaaagtg agataagaac cctgttcagt cttttgtacc
     5821 gaatccataa tcacctgttc cagctttgga tccagagtta taacatttga ttttttatcc
     5881 ttgataaatc tttttgaaat tgcccttcct aaagcctgcc ttacatattc ggtcagaagg
     5941 tctgtatcat gagttgaagg tgcatagtct gccagggttt ccagtatcgt taccatatcc
     6001 ctcacagata ccccttcttt taataggttc gcaattacct tttgtatttc tccaatggtc
     6061 atcattttag gaacaagttc ttcgacaata gccggataat tctcctttac cttgtcgata
     6121 attgtctgaa cttcctgcct cccggttaat tcatgggcat gggatttaat cacttcagtc
     6181 aaatgggtgg caataatgga gggcgaatcc acaacagtat atcccagcat ctctgccctg
     6241 tctctctgtg cctcgctaat ccacacagcc ggtaatccaa aagccggttc ggtggtcttt
     6301 attccgtcaa tctcctcctc cacaaccccg ggactcatag caagatagtg atcaagcatc
     6361 aactctccac caccaacttc aactcctttt attttaatta tatattcatt agggcttagc
     6421 tggatattgt ctctaagcct tattatcggc actatcattc ccaattccaa ggcaagctgc
     6481 cgtcttatca ttacaaccct gtctaaaaga tctcctccct ggttaacatc agcaagaggt
     6541 ataataccgt atccgaattc cagttcaatg ggatcaacct gaagaagatt tacaacattt
     6601 tcaggttttc tgatctcctt gacttcgttt tcctcaatct ggattccttc ctgcttttca
     6661 ctttctgcgt ttttccttat gaggattatt ccaatagcaa taaatactgc tgccagagta
     6721 agaaaaggca atgtcgccaa gggtatggcc aacaatatac aaaatccggc tgctataaag
     6781 aacacattgg agttgtacaa aatctgtttt ttcaagtcga cacttaatcc ctcatcggaa
     6841 gtagatttcg taacaataag gccggtagca acagagataa tgagtgcagg tatctggctt
     6901 acaagcccgt ctcctatggt cagtattgtg tagttctgaa aagcatagct tatttcctcg
     6961 tttctcataa ccgcaccgat aatcacccct cctataacgt taattatggt gataataagc
     7021 cctgcaatgg catctccctt tacaaattta gtagcaccat ccattgctcc gtagaaatcg
     7081 gattctctct gaatcttcct ccttctctcc ttagcttcca tctcatttat caatccggca
     7141 ttcaaatcgg catcaatagc catttgctta cccggcatgg catcgagtgt aaaccttgcc
     7201 gcaacctccg acactctttc tgttcccttt gtaattacaa gaaattgaac gatagtaatg
     7261 attaaaaaga ttacggctcc caccaccagg ttcccttggg caacaaagtt tccaaatccc
     7321 tcaataacct gtcctgcttc tccgtttgcc agtatcaaag ttgttgactt aatatttaat
     7381 gcaagcctgt ataatgtcgt aaacaacaag accgaaggaa acactgaaag ctgaattgct
     7441 tcctttgaat aaaccacatt aagaagaatt actattgcca acgcaatatt cagtgcaaga
     7501 aggacatcaa gaattccact ggggacaggc aagataatta ccagtacaat agatacaact
     7561 ataactgcta tcgaaatttc cccaaattta accctcatgt catttcactc cttcgtgcca
     7621 tattaagtaa attatcagca aaagcctgca tctataccat cagccttatg attttatcta
     7681 tttcttttaa gtccgtatac ataagcaaga acttcagcta cagcctgata tagctccgga
     7741 ggtattttcc cgcctatttc cacagtctca tacagagttc gggcaagggg tttgttttca
     7801 acaatttcca cattgttctc ctttgctatt tcctttatcc taagtgcgat ataatcctgt
     7861 ccttttgcca gaacaacagg tgcatccgct acttttgaat catattttat tgcaaccgca
     7921 aaatgcgtcg ggtttgttat aacaacatca gcattcggta tgtcctgaag cattctcctc
     7981 attgacatct gtctctgctt ttgccttatc cttgattttg tttgcggatt tccttccgtc
     8041 tccttatact cttccttgac ttcctgcttt gtcatcctta ggctcttttc atactcccac
     8101 cactggtaac catagtcaat cactccaata aaaagcattg ctatacccat tcttatacct
     8161 acgtttgtag ccgtaacacc tatataggat gctatactga ttacgtccat gttcattgtg
     8221 ctgagcatat ccttcgattc ccctttgatg tatgagtaac cgacgtaacc aactacagca
     8281 atctttatta tagactttgc aaggtccacg agggatcttg cggaaaaaat ccttttaaac
     8341 ccattcagag gattaatctt gcctaacttg acgcccagcg tctttgttga aaataaaaac
     8401 cctacctggg caaaacccga taccaatcct gtcaataacg ctatagcaaa caaaggagct
     8461 actattttca gcattgttat aagggtctca tcaaacattt tcattacgcc gtctatggta
     8521 aacaggttgt cttcctcaaa ataaacagta aaaacctttt tgaagcaacc tagaatttcc
     8581 tcatacataa atccgccaaa agcccttaac cctataaaca tacacagaag aacaattgca
     8641 gcagtcattt cccggctttg cagcacctgt ccctctttcc ttgccttcga cttctttctc
     8701 ggagtggctt tttcagtttt ttcaccactg tctgcaaaga gctgcaggtt aactttcagc
     8761 ttttctccac ctgcacaaag gcttcctcgg tcaccgcctg cagcggtttg acatttgtca
     8821 tccataaatt caattattgt tttaattatg cctgcacttt ttactttcat tttggtgcca
     8881 tatccttaag aaaagtatac atttcaccat ccatacccct tataaggctt tccatgaggg
     8941 atatgaatac cggtatcgtt aaaaccatta cagctaagcc aagagttatc ttaagcggca
     9001 ttcccaccat aaaaacattc aattgcggca ccgtttttga gagaactccc agggcaacat
     9061 cggttataat tattgccgca agaatcggtg cactgattct aaaagctata aaaaatatgt
     9121 ttccgaatac ccttaaaata tcgttgacaa tttcttttcc aaacaccccg gtacccagcg
     9181 gtatatagtt gtaactttta aaaagtgccc ttattaaaat atggtgtcct ctgcacatca
     9241 ggaaaataag catacataat ataaagtaaa aattcgacgt aataggtacc tggatattac
     9301 tcatcggatc aataatgtta acaacaccaa atccaatctg catgtcaata atatgtcctg
     9361 cgaggtatat agccgtaaaa atcaagtaag atacataacc taatgtaata cccaccaaaa
     9421 actctttcaa tacaagtata aagtactcat agatattgct gaaaacgagc tcctgaacct
     9481 ttatggtgtt tgcaagaatc agtgccagca taaatgaaaa accaattttc aaatacgcag
     9541 gtatgttcct tcctccaaaa atgggagcta taacaaacag gcctgtcatt ctaacgaata
     9601 ttaacaagaa tatttctata ccgctgtaca aaattccttc tggaatagcc aatttgttgt
     9661 cctccgattg aaaattcttt tcttacctaa tgtactgatt tatattcaag tatatattct
     9721 gagtaaaatc aatcaaaacc cttaacatcc aagccccaaa gattacaata ccgcttaaaa
     9781 tagccagtat tttgggtata aaggtcagtg tctgctcctg aatctgg
//
LOCUS       DAOOJC010000028         9722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_6221_length_9722_cov_3.916106, whole genome shotgun sequence.
ACCESSION   DAOOJC010000028 DAOOJC010000000
VERSION     DAOOJC010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 9722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9722
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6221_length_9722_cov_3.916106"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="PLA01_01525"
     CDS             complement(<1..1215)
                     /locus_tag="PLA01_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPD00012.1"
                     /translation="MTKEYEVINNVETLEKAIKRLRKAQDVFSTYTQEQVDRIFYAAA
                     MAANKMRIPLAKMAVEETGMGVVEDKVIKNHYASEYIYNAYKNTKTCGVIEEDPAFGI
                     KRIAEPVGVIAAVIPTTNPTSTAIFKTLIALKTRNAIIISPHPRAKNSTIEAARIVLE
                     AAVEAGAPEGIIDWIDVPSLELTNMVMREVDIILATGGPGMVKAAYSSGKPAIGVGAG
                     NTPAIIDDSADILLAVNSIIHSKTFDNGMICASEQSVIVLEGIYKEVKKEFADRGCYF
                     LNKEETEKVRKTIIINGALNAKIVGQKATAIAKLAGVDVPESTKILIGEVTSVDISEE
                     FAHEKLSPVLAMYKAKDFDDALNKAERLIADGGLGHTSSLYINTVTEKEKIDKFAARM
                     KTCRVLINTPSSH"
     gene            complement(1233..1877)
                     /locus_tag="PLA01_01530"
     CDS             complement(1233..1877)
                     /locus_tag="PLA01_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPD00013.1"
                     /translation="MNPKSISKQTLLRLPSYLSYLKSLPEGDNEHVSATMIATALGLN
                     DVQVRKDLACVSKKGRPKLGYVTGDLIKDIESFLGYDDLNDAIIVGAGRLGGALMSYE
                     GFKEYGLNIVAAFDTDERVVGTEICGKKILPMDKIKDLCESLKIRIGIITVPAHAAQK
                     VCDMLVESGIYAIWNFAPTHLKVPDNILVQQENMAASLAVLSAHLAEAIKKNGF"
     gene            complement(2206..4074)
                     /locus_tag="PLA01_01535"
     CDS             complement(2206..4074)
                     /locus_tag="PLA01_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPD00014.1"
                     /translation="MKMKGLISKRKWAMIVSFVILATMIVSLNTINLNAAAEVNFAKA
                     LQYSMFFYDANMCGTGVDENGLLDWRSNCHVYDAELKLDSQNTNMSDGFISSNKSILD
                     PDGNGKVDVSGGFHDAGDHVKFGLPEAYAASTVGWGYYEFRDQFIATGQHTHAEVILR
                     YFNDYFMKCTFRDSSGNVVAFCYQVGDGNIDHSFWDSPENDSMPRKGWFATKELPSTD
                     VISATSASLTVNYFNFKDTDSQYAAKSLDYAKALFDFAKKNGKSVNDQGCGGFYNSSK
                     WQDDYCWAAAWLYLATQEDDYLEEAFKYYDYYAPPGWVHCWNDVWSGVACILAEANDL
                     YDNDSQAFEDRYKKASNKNPYEEIDFWKPIEDMVNKWMTSVPKTGGGYAFLDQWGSAR
                     YNTAAQFVALVYDKHHGDTPSKFGNWAKSQMEYLMGNNPINRCYIVGYSSNSAKYPHH
                     RAASGLKDCHDQSPHKHVLYGALVGGPDLGDQHIDKTEDYIYNEVAIDYNAAFVGACA
                     GLYRFFGDSSMKIDPSMPSHSIPDPTPMPTPTPTESAGLYGDLNGDEAVDSTDLTLMK
                     RYLLRKIGGSYISELLADLNGDGVIDSTDLTILKRYLLRKITKLPIEPAGGGPVID"
     gene            complement(4494..6509)
                     /locus_tag="PLA01_01540"
     CDS             complement(4494..6509)
                     /locus_tag="PLA01_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPD00015.1"
                     /translation="MDIKWRNIKYKKGTKVLAVILAWLCFLSVFGGAVFLIQNNLIIS
                     CESFYSYPKNVSNFSSHVLSVVDYYIWVKGAKSEEEIRASSTEEDGSIVDYKIVAFIN
                     STRTLSKVVNFGYYIKNAETGEIITNVKEDNPIELLKKQHHYVYINKSELSNDWIYYE
                     YEIMKRMEGTPYEVHAAVIEPLKPGDEFYDDYIQYNRIKTTSAIVIPSMIIGFILMLV
                     VLAYLCFTAGKNEKGEKLPLKFLDRIYIDVFTLFVLMLLAAYAYCFIQTMDSFYVNEI
                     GSLFIIIVVLSLAFFTGLSYSLSLVRRKRAGKVTENILVMKVLRKIKEFKKLCFNGKI
                     FRAWILLLLPVYVFANAILAYIIGKYSLYYYDTKKFYYMVFLLAGINAVAFFFTAKAL
                     KSLSVIMEGTKQISRGNLDYKMNSDKMSPVFAAFAENICNIQGGLKKAVDDAIRGERM
                     KTELITNVSHDLKTPLTSIINYVDLLKTEKIENKKAQEYIGILEEKSARLKALIEDLV
                     EASKASSGNIAVNLEKVDLHELVNQAYGEYQEKAERAGLDIRINASDKNILVLADGKH
                     MWRIVENLLSNVVKYSMPGSRVYINISKNETDGILTIKNISALPLEVPAEQLTERFVR
                     GDTARTTEGSGLGLSIAQSLAIVQKGKFNIEIDGDLFKVIVAIPLWK"
     gene            complement(6484..7179)
                     /locus_tag="PLA01_01545"
     CDS             complement(6484..7179)
                     /locus_tag="PLA01_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPD00016.1"
                     /translation="MDKFNILVCDDDKAIVDALEIYLRQENYNVIKAYTGKQALKALE
                     QEKIHLVIMDVMMPELDGLTATVKIREELNIPIIMLSAKSEDTDKIIGLNFGADDYVT
                     KPFNPLELIARVKSQLRRYTLLGGAAEKKGVLKTGGLELDVEAKELRIDGEVVKLTAT
                     EYGIVLFLMQNMGKVFSIDQIYENVWNEPSYSSENTVAVHIRRIREKIEINPKEPKYL
                     KVVWGIGYKMEKY"
     gene            complement(7404..9152)
                     /locus_tag="PLA01_01550"
     CDS             complement(7404..9152)
                     /locus_tag="PLA01_01550"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HPD00017.1"
                     /translation="MGYQIFTNILEISLIGSILILLALGIKKVLGSRLFPKAYLIMWL
                     NIFLRLIVPFQMEGGFSLADKITFLSFEQKNQADKDEKITMTDVPSGNQPVNETDSKN
                     PYTQNSYDADKNAVEDSDSIARQDNIDIVQKSWDIFKVLLLVWLIGFCMILAIMAAIF
                     FGTYRRILKSRVCEREDIIELVEECKSSIKLNKNVGIILSDLLKGPGVIGVFKPQLVL
                     PEAFLEYRDETGKRNIILHEMYHIKYCHNLLNIVFLAVACIHWFNPLVWVALFKSRTD
                     IESVCDEAVVKVLSDDGVEQYAKTIIQYAASAKRSPSILLHTGMIGGKNAMKSRIASI
                     SSYGRRSIKWLIVSLLLVTVLGSCGIIGSFEGEGEKVSSNPPVNTGQGDLRNSEDANE
                     IINDGSPEDESQLNSGTVEENPSSSDDEDPLSFIANEAADEYFLNTEKGKISLMVWDN
                     AIDLNGLLGEPISEEVIELTNADTFTGSFDKTIKYDGLEIKLFSPKGNGKSFYVYKVK
                     TTNPIYTSARGVKVGDTVETLKEKYPEVIMALDGRTDEMNCAYLYPEKRDKYLNLFFE
                     VNNGKVEEIYMYYEFP"
     gene            complement(9157..9534)
                     /locus_tag="PLA01_01555"
     CDS             complement(9157..9534)
                     /locus_tag="PLA01_01555"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HPD00018.1"
                     /translation="MKKVPSIGEAEWQVMEVLWEMGRANFTEIRIALKAFDWTQTTIH
                     TMLQRLHKKGLIDIEPDSSPYVYYPLVSKDACRKHETKTFLQKVYNGSLKLLISNFIH
                     EADLTEDDIDELMRILKNRKKEG"
BASE COUNT         2775 a         2226 c         1520 g         3201 t
ORIGIN      
        1 gtgggatgac ggagtgttaa tcaacactcg gcaggtcttc attcgtgccg caaacttatc
       61 aatcttctcc ttttcggtca ccgtatttat atacaacgat gatgtatgtc ccaagccacc
      121 gtcggcaatc aacctttccg ccttgttcag ggcatcgtca aaatcctttg ccttgtacat
      181 tgccagcacg ggagacaatt tttcatgggc aaactcttcg gaaatgtcca cactggttac
      241 ttcaccgatt agaatttttg tggactcagg tacatcaaca cctgcaagct tcgcaattgc
      301 agtagccttt tgccccacta tttttgcatt caatgcaccg tttatgataa tagtttttct
      361 aaccttctcg gtttcttcct tgttcaagaa atagcagccc ctgtcggcaa attccttctt
      421 aacctcttta tatatgcctt ccaaaacaat gactgactgc tccgaagcac aaatcatacc
      481 gttgtcaaag gtttttgaat gtataattga gttcactgcc aaaagaatat ccgcagaatc
      541 atcaattatt gcaggagtgt tccccgcacc aaccccaata gccggtttac ccgaagaata
      601 agccgcttta accattccgg gaccgcctgt tgcaagaatg atatccacct ctctcattac
      661 catattggta agctccaggc tgggcacatc tatccaatca ataatgcctt ccggtgcacc
      721 ggcttcaact gccgcttcca aaacaatcct tgcagcttca attgttgagt tctttgctct
      781 cggatgagga cttatgataa tcgcatttct tgtcttcaaa gcaataagtg ttttaaaaat
      841 agccgttgac gtaggattgg tggtaggaat aactgccgca attactccca ctggctctgc
      901 tattctttta atcccgaatg cagggtcctc ctcaataaca ccgcaggttt tcgtattttt
      961 atatgcatta taaatatatt ccgacgcata gtggtttttg ataaccttat cctccactac
     1021 tcccatgccg gtttcctcaa cagccatttt ggcaagcgga atcctcattt tattggcagc
     1081 cattgccgcc gcataaaaaa tcctgtcaac ctgctcctgt gtataggttg aaaaaacgtc
     1141 ctgtgctttc cttaaccgct ttattgcttt ttccaatgtt tcaacgttgt ttatgacttc
     1201 gtattctttt gtcattgttt acatccccct tcttaaaatc cattcttctt tattgcttct
     1261 gccaaatgag cagatagaac tgcaagcgat gctgccatat tctcctgttg gaccaaaata
     1321 ttatccggta ctttaaggtg agtaggtgca aaattccata ttgcatatat accactttct
     1381 acaagcatat cacatacctt ttgtgccgca tgggcaggaa ccgttatgat gccgatacga
     1441 atcttcaaac tttcacataa atccttgatt ttgtccatgg gcaatatctt ttttccgcat
     1501 atttcggtac cgactactct ctcatcagtg tcaaatgcgg caacaatgtt taacccatac
     1561 tccttaaacc cttcatagga catcagagct cctcccagcc ttcccgcacc tacaattatt
     1621 gcatcattca aatcgtcata ccccagaaag ctctcaatat cctttattaa gtccccagtt
     1681 acatatccaa gcttcgggcg gccctttttg ctcacacagg ccagatcctt tctaacctgt
     1741 acatcattta gccccaatgc cgtggcaatc atcgttgcag aaacatgctc gttatcacct
     1801 tcaggcaaag actttaagta gcttaagtat gaaggtaatc ttaaaagagt ctgttttgag
     1861 atacttttcg gattcatagt ttcataagtt cctttcttac tgtttgtcaa aattaaaaac
     1921 ctgtcttgta tcaccctacc gttttctcat ttttgcattt ctaaattctg ctgcaatttt
     1981 gtttgatatc cgtattttta tatcgataat tttataccga tatttattta tcgatattat
     2041 tatactgtta atttattaac aatgtcaact ggttttgtgt atattttgga atccacaaaa
     2101 tgccattatg tcgtataaaa aactcagggg aatcaaaccc ctgagccctt tgcgattatt
     2161 ataatcataa tcactaaatc atttttactt tattaaacaa taatctcagt caatgaccgg
     2221 tccgcctcct gcaggttcta taggaagttt cgttatttta cgaagtaaat acctttttag
     2281 tattgtaagg tctgttgaat caatcactcc gtctccattt aaatctgcta acagttccga
     2341 gatatacgaa ccaccaattt ttcttaataa gtatcttttc ataagtgtca aatcagttga
     2401 atcaactgct tcatctccgt ttaaatcacc atacaaacct gccgattcag ttggtgtcgg
     2461 tgtcggcata ggtgtcggat ctggtataga atgcgaaggc attgacgggt ctatcttcat
     2521 tgaagaatct ccaaagaagc ggtataaccc tgcacatgct ccaacaaatg cagcattata
     2581 gtcaattgca acttcgttat aaatatagtc ttctgtcttg tcaatgtgtt gatcgcctaa
     2641 atcgggacca ccaaccaatg caccgtatag tacatgttta tgcggagact ggtcatggca
     2701 atcctttaag cccgatgccg ccctatggtg cgggtatttt gccgaattac tgctgtaacc
     2761 tacaatataa caacgattta ttgggttatt acccattaaa tattccattt gagactttgc
     2821 ccaatttcca aatttggacg gggtatctcc atgatgcttg tcatatacaa gagcaacaaa
     2881 ctgggctgca gtattatatc tggcagaacc ccactgatca aggaatgcat atcctccacc
     2941 tgttttagga actgatgtca tccatttatt aaccatatcc tctatgggct tccagaaatc
     3001 aatttcctca taaggattct tattggaagc tttcttatac ctgtcttcaa atgcttgact
     3061 gtcattgtca tacaaatcat tcgcttcagc cagaatacat gctactcctg accatacatc
     3121 gttccagcaa tgtacccatc ccggaggagc atagtaatca taatatttaa atgcttcttc
     3181 caaataatca tcttcttggg ttgccaaata aagccatgct gctgcccaac aataatcgtc
     3241 ctgccatttg gaagaattat aaaagcctcc gcatccctgg tcattaaccg attttccgtt
     3301 ctttttcgca aaatcaaaca aagcttttgc ataatcaagg ctcttagctg catattgaga
     3361 gtctgtgtcc ttgaaattaa agtagttgac tgtcagagaa gctgacgttg ctgaaataac
     3421 gtcggttgaa ggcagctcct tggttgcaaa ccatcctttt ctcggcatgg agtcattttc
     3481 cggtgaatcc caaaaagaat ggtcaatgtt accgtcaccc acctgataac agaatgccac
     3541 cacattaccg gagctatccc ggaaggtaca tttcataaaa taatcattaa agtacctcaa
     3601 aattacttct gcatgggtat gttgtcctgt tgctataaac tggtctctaa attcgtaata
     3661 accccatccc actgttgaag cagcgtaagc ctcaggcaga ccgaatttta catggtcacc
     3721 cgcatcgtga aagcctccgg aaacatcaac tttaccgttt ccatcagggt caagtatact
     3781 cttgttactg cttatgaaac cgtcggacat attggtgttt tgagaatcca gcttaagctc
     3841 tgcatcataa acatggcagt tgcttctcca gtcaagtaaa ccattttcat caacacctgt
     3901 accgcacata ttagcatcgt agaaaaacat agaatactgc aaagcctttg caaagttgac
     3961 ttctgcggca gcattaaggt tgatggtatt aagagaaaca atcattgtag ccagaatcac
     4021 aaaactaaca atcatagccc attttctctt tgaaattaac cctttcattt tcatcctatc
     4081 ccttccttcc aaaaaatgat tttatgtgaa ttaaataaag aaccaattct gccacaaggc
     4141 actggttttt ttgacagtac tgtaattctt cataactgca tggcaaaaca tactttatta
     4201 ttggaaaata aatatagagt aagacagaat cggtattaag accatgataa gacggctttt
     4261 ataaaacaat cggaattatt tgttcatatt tggaagaatc gtgttgctgc aaataataat
     4321 attcattatt caatatatca gtacttatat aattatattt taattatccg taaagataaa
     4381 attgtgcctt aaaaaaatta accccgttac aagacacaac tttacccttc atatttatct
     4441 tactatattt tttatcacat tacaattagt ttatcttaac tagttcacta cagctatttc
     4501 cacaaaggta tcgccacaat caccttaaat aaatccccgt ctatttctat attaaatttc
     4561 cccttctgaa caattgccaa actttgagca atggacaagc caagccccga cccttccgtt
     4621 gtccttgctg tgtcacccct cacaaacctt tcggtcaatt gctctgccgg tacctcaaga
     4681 ggaagtgccg atatgttctt tatggtcagt ataccgtctg tctcattttt tgatatatta
     4741 atatacactc ttgagcccgg catcgagtat tttacgacat tcgacaaaag gttctcaact
     4801 atccgccaca tatgcttccc gtcggccagc acgagtatgt ttttgtcgga agcgttaatt
     4861 cggatatcaa gccctgctct ttcagctttt tcctgatact ctccatacgc ctgattaaca
     4921 agctcatgca aatccacctt ttccaaatta actgcaatgt ttccgctgga tgctttgctg
     4981 gcttccacca aatcctcaat aagtgcctta agtcttgcgg atttttcctc aagaatgcct
     5041 atgtattctt gtgctttttt gttttcaatc ttttccgttt tcagcaaatc aacatagttt
     5101 attatagagg tcagcggcgt cttcaagtca tgggatacat tagtgatcaa ttcagttttc
     5161 atgcgttctc ccctaattgc atcatccaca gcttttttta atcctccctg aatattgcag
     5221 atgttttcgg caaaagcagc aaatacaggt gacatcttat cgctgttcat tttgtagtcg
     5281 aggttgcctc tggatatttg tttggttccc tccataatta cggaaagaga cttcaaggct
     5341 tttgctgtaa aaaagaaggc tacagcattt attccggcca gcaaaaaaac catgtaataa
     5401 aattttttcg tgtcataata ataaaggctg tatttaccta tgatataggc taaaatagca
     5461 tttgcaaaca cataaaccgg gagcagcaaa agaatccatg ccctgaatat cttaccgttg
     5521 aaacaaagct ttttgaactc ttttattttt cgcaaaacct tcataaccaa tatgttctct
     5581 gtgacttttc ctgccctttt ccgtctcact aatgacaggg aatagctcaa cccggtgaag
     5641 aaagccagac tcaatacaac tataatgata aataaacttc ctatttcatt gacgtagaat
     5701 gaatccatgg tttgtatgaa acagtaagca tatgctgcca ataacataag cacaaacaga
     5761 gtaaatacat ctatatatat tctgtctaaa aatttcaggg gtaatttctc tcccttttca
     5821 ttttttccgg cggtaaaaca aaggtatgcc agcaccacta acattagtat aaaacctatt
     5881 atcattgaag gaattacaat cgctgatgtt gtcttaatcc tattatattg aatataatca
     5941 tcgtaaaatt cgtcaccggg ttttaatggc tcaatgactg cagcatggac ttcatacggt
     6001 gtcccttcca ttcttttcat tatttcatac tcataatata tccaatcatt gcttaattca
     6061 cttttattta tatacacgta atggtgctgt tttttcaata attcaatggg attgtcttcc
     6121 tttacatttg ttataatttc gcccgtttct gcattcttga tataataacc aaaattcaca
     6181 actttagaaa gcgttcgagt tgaattaata aaagcaacta ttttatagtc aacaatgctg
     6241 ccatcttctt ccgtactcga agccctaatt tcttcctcac tttttgcacc tttcacccaa
     6301 atgtaatagt ccacaacgct taaaacatgg gatgaaaaat tagatacatt cttcggataa
     6361 ctataaaaac tttcacaaga tatgatcaga ttattttgta tgagaaagac agcacctcca
     6421 aatacggaca agaaacacag ccatgccaaa ataactgcta acaccttcgt acccttttta
     6481 tatttaatat ttctccattt tatatccaat accccacacc acctttaaat atttcggttc
     6541 tttcggatta atttcgattt tctcccttat tcgccggatg tgcacggcaa ctgtattttc
     6601 cgacgaatat gacggttcat tccacacatt ttcatatatt tgatcaatgg aaaacacctt
     6661 gcccatattc tgcataagga ataaaacaat gccgtactct gttgccgtca gctttactac
     6721 ttctccgtca attctcaatt ccttcgcctc tacatcaagc tccagcccgc cggttttcaa
     6781 tacccctttc ttttctgccg ctcctccgag caaagtgtac cgtcttagct gtgactttac
     6841 tcttgcaata agctccaagg gattgaaagg ctttgttaca taatcgtcgg caccaaagtt
     6901 tagcccaata atcttgtctg tgtcctcaga ctttgctgat agcattatta tgggtatatt
     6961 taattcttcg cggattttta cggttgcagt caaaccgtcc aattcaggca tcatcacatc
     7021 cattataacc agatggattt tttcttgctc caaagccttg agtgcctgct ttcccgtata
     7081 tgccttaata acattatagt tttcctgcct caagtaaatt tccagtgcat caactatcgc
     7141 cttatcgtca tcgcatacca gaatattaaa tttatccata tgtacaggcc cccagtcctt
     7201 tttcaaaata atacatgccg catcacctgc cttttttctt ttagcatatc aggcagattt
     7261 tacaaaacac ttcataaaat tcttaagatt ttcttaagat ttggtaatta gtttaaaaac
     7321 atattaatgg gtgaaatatt taaagccact gaaaattata ccttttttca gtggcttttt
     7381 aaatcattat aactcactaa tttttatgga aattcataat acatgtatat ttcttcaacc
     7441 ttcccgttat tcacctcaaa aaaaaggttc agatatttat cacgtttctc aggataaaga
     7501 tacgcacaat tcatctcgtc cgtccttccg tcaagagcca taataacttc aggatatttc
     7561 tctttcaatg tctctaccgt atcccctact tttacgcccc ttgcactggt gtagatcggg
     7621 ttggtggttt ttactttata tacatagaaa ctctttccgt ttccctttgg agagaacaat
     7681 ttaatttcca acccgtcata ctttatggtt ttatcaaagg aaccggtaaa tgtatctgcg
     7741 tttgtaagtt ctatcacttc ctccgatatt ggttcgccca acaacccgtt taaatcaatg
     7801 gcattatccc aaaccataag tgaaattttc cccttctcgg tatttaagaa atattcatcc
     7861 gccgcctcgt tagctataaa ggaaagggga tcctcatcat cggaggagct gggattttcc
     7921 tccacagttc ctgaattcag ttgggattca tcctctggtg agccgtcatt tattatttcg
     7981 tttgcatcct cagaatttct caaatcacct tgcccggtgt ttaccggcgg attgctgctg
     8041 accttttcac cttcaccttc aaaagagcct ataattccgc aagatcccaa aaccgttaca
     8101 agcagcagtg atacaataag ccacttaatg cttcttcttc cgtatgaaga aatacttgct
     8161 attctggatt tcattgcatt tttccctcct atcattcctg tgtgcaataa tattgaagga
     8221 cttcttttgg ctgatgccgc atattgtata attgtctttg catactgctc cacaccatca
     8281 tctgataaaa cctttacaac cgcttcgtcg cacacagact ctatatccgt tctggactta
     8341 aacaatgcca cccataccaa tgggttaaac cagtgaatgc atgccactgc aagaaaaaca
     8401 atattcaata ggttgtggca gtactttata tggtacattt catggagtat tatatttctc
     8461 ttgccggtct cgtcccggta ctccaaaaaa gcttccggca aaaccaactg aggtttaaag
     8521 actccgataa caccgggccc cttcaataaa tccgacagaa taataccgac atttttatta
     8581 agcttaatgc tgctcttaca ctcttcgacc aactctataa tatcttccct ttcacatacc
     8641 cggcttttca aaatcctgcg gtatgttcca aaaaatattg cagccataat ggctaaaatc
     8701 atgcaaaagc ctattagcca gacaagtaaa agcaccttga aaatatccca actcttttga
     8761 actatatcga tattatcctg acgagcaatg gaatcagaat cttcaacagc atttttatca
     8821 gcatcatatg agttttgggt atagggattt ttactatccg tttcgttcac tggttgattc
     8881 ccggatggaa cgtctgtcat ggtaattttc tcgtctttgt ccgcctggtt tttttgttcg
     8941 aaagaaagaa aagtaatttt atcagctaaa gaaaacccgc cttccatctg aaaaggaaca
     9001 ataagcctta aaaatatgtt tagccacata attaaatatg cctttggaaa aagcctgctt
     9061 cctaaaacct tctttatacc caatgccaat aaaataagta ttgatccaat caaagatatt
     9121 tcaagaatat tggtaaatat ttgatagccc atccttctac ccttctttct ttctgttctt
     9181 caatattctc atcagctcat ctatatcgtc ctccgtcagg tctgcctcat gtataaagtt
     9241 ggatataagc agctttaacg aaccgttgta aaccttttgg agaaaagttt ttgtttcatg
     9301 ctttctgcat gcgtcctttg atacaagagg ataataaaca tagggagagg aatccggttc
     9361 tatatcaatt aatccttttt tatgaaggcg ttgcagcatg gtatggatgg ttgtctgagt
     9421 ccaatcaaag gcttttagag caattcttat ttcagtgaaa ttagcccttc ccatttccca
     9481 caatacctcc atcacctgcc attcagcttc acctatgctt ggaacctttt tcataaaacc
     9541 acttcccgtt tataaattat cataatacca ttctacatat gtagtattat aattgtcaag
     9601 taggattttt tgttttgata ttagtttaat tatctttatt ggcactttta gccctaagcg
     9661 aataaaatgc atttatatag aattggaaac caatatcaaa ataagggttt agttgataaa
     9721 tt
//
LOCUS       DAOOJC010000029         9627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_6269_length_9627_cov_3.525909, whole genome shotgun sequence.
ACCESSION   DAOOJC010000029 DAOOJC010000000
VERSION     DAOOJC010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9627
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6269_length_9627_cov_3.525909"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            438..1586
                     /locus_tag="PLA01_01560"
     CDS             438..1586
                     /locus_tag="PLA01_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD00019.1"
                     /translation="MGKLNLVVADTDEAYVENIVNYFMTNHSQRFQISSFTKHEFLYD
                     FLSELREIDVLLVSPEMYTESLPKEGIIEIFILTSGELPEKCKGLNHIDKNQIGDGFV
                     KEIADKYPEDEDNAIEEPIVEEPVSVEQAIKDRKTKVVAIYSPLGGVGKTTIAVSSSI
                     YCGKKGLNVFYLNFESFQSTPLFFNCKNEKSYSKILEYVKDKRDDLHRMIEASKQVDT
                     DHNVYYLSPPESILDILEAEPKDFRRLINEFKSMGMYDIVIVDMSSELSDRNIALLEA
                     CDQVVLVLAQDAISNIKAESFSQQLEALLKRKGLDFTGKLTLVLNKYNFHMALEVETV
                     DINEEFISIYIPVVPGMTAIKGNAQLVDLQGEFGASIQELMDKIILHS"
     gene            1733..2443
                     /locus_tag="PLA01_01565"
     CDS             1733..2443
                     /locus_tag="PLA01_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HPD00020.1"
                     /translation="MAGRLEKTYGKAFKYLLFAINPFKRLVIKTEAAIHQLINLQALE
                     ILKNDKYIDAHCLFSDYIEDINKGAVWADLDIKSVNHFYNPVKKKGLYGNDNALTLAV
                     RYYGNAKEYWYKDKPNTSMFYLGAAVHLVQDMTVPHHANIRLLDNHRQYENFIRKTYL
                     NTPKFLADRGGYYLSGIEEFITCNARNAIKIYMRLKDIEDASKRYYTIAKFTIPLAQK
                     TTAGCFINFYRDISKRII"
     gene            complement(2584..3240)
                     /locus_tag="PLA01_01570"
     CDS             complement(2584..3240)
                     /locus_tag="PLA01_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcN/YlaJ family sporulation lipoprotein"
                     /protein_id="HPD00021.1"
                     /translation="MNKNFLKATSLTLGLTLASLPLVGCNMGGGTARRNQPEATGQNT
                     QQNNQSVNPNRFLNMDLNPMDNKGANNNNGMNNNAKSGLLGRIDTTPMGNPAAPAAPT
                     PGVQSPEEMKQKSENITNQLDAMPEIDKVNALVTGNTCLVGYSPANAQGDPNARKNMV
                     IEKVKQIDPNVNNVVASESQDVMNRISQLLNDIANNRPMDEINREVMQLMKTTAPNIS
                     "
     gene            3441..3857
                     /locus_tag="PLA01_01575"
     CDS             3441..3857
                     /locus_tag="PLA01_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00022.1"
                     /translation="MYYKECMPCMMNPMAMPQHCGPMMPMMQQQLESMYPRTYHIVYP
                     RVQYHCDMLDMNYGCAYCPTQGQVDDMCDRICKEVEKDIEACMKDDMREEGDRQIGFG
                     GRFLLNAFIRTLIIRELLGRRRRFGPPFFGFPGFGF"
     gene            complement(3946..5310)
                     /locus_tag="PLA01_01580"
     CDS             complement(3946..5310)
                     /locus_tag="PLA01_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HPD00023.1"
                     /translation="MTDIFPYDKNLGIQIPSIMLPKKGVDMTLWSVVACDQYTSEPEY
                     WNKVNEIVGDNPSTLRLTFPEVYLGDGSETERIKKINSEMKKYISEGTLVSDDPCMIF
                     VDRKTSHVQSRKGLILSVDLEKYDYTKGSQTLIRATENTVIDRLPPRIKIRENASLEL
                     PHVMLLIDDPQKTVIEPLEQKLDSFEKVYDFELMMNGGHLKGYKINDQKTLDGIFKAI
                     NDLADPQAFRDKYGLRDDKGVLLFAVGDGNHSLASAKGHWENVKKNLSAQERENHPAR
                     FALVEVVNVHDDGITFEPIHRVLFNIDPEAILKDMADYFEKYGGAFEKFDSLESMKNK
                     LSELTKDRSCHNFGFVNASGFGVFTIRNPECNLEVATLQCFLDDFIKSNRDSKIDYIH
                     GEDVVTRLGSKAGNIGFYLPAMRKHDLFKTVILDGVLPRKTFSMGEAEEKRFYLECRR
                     IVKD"
     gene            5857..6426
                     /locus_tag="PLA01_01585"
     CDS             5857..6426
                     /locus_tag="PLA01_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HPD00024.1"
                     /translation="MLRYSEVIGLPVICATDGKRLGVIKDIVFCPKDKKVVAFLVERK
                     GYEILKKVVFMKDIQSMGKDALIINSPKDIKDLKKVQDSNELKEKGDILGLRVCTRSG
                     EDVGVAKDVLLDFTHGKIDGIEVSGGLIQDILEGRNILPLIGKVEFGEEYILVDSESI
                     EEMMETGGGIKNIIEGKKRFQERGDFDAK"
     gene            6416..6598
                     /locus_tag="PLA01_01590"
     CDS             6416..6598
                     /locus_tag="PLA01_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HPD00025.1"
                     /translation="MRSNFTRGLVVGSIIGASVGVAMNSGKMMNNRSRRRIKNRGLDI
                     MRKSGALISDVVELFR"
     gene            6710..7759
                     /locus_tag="PLA01_01595"
     CDS             6710..7759
                     /locus_tag="PLA01_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPD00026.1"
                     /translation="MIGIRRVVSYIVLFLIGAAIILFVTTYRDKLVKIFFPIVLAGIL
                     AYIIRPLVLKLEKRNISRTKGILLIYLVSGIVLIVSVIFIVPVLVNNTREFINTFPEL
                     TLEYKNNFNNVVKMIDTSNWPPEIKNAVFREINNTAGILETKILNALRNALFGSINAA
                     ARIGDFIIAMIIAYYLIKDAEFFKRNFLSLVPKGWRNNAIATCREINDILSSFIHGQI
                     LTAVIIGIMEATVLLIIGIKYAPILGIIGGIANIVPYFGPFIGAIPAVAVAFIDSPVK
                     VIWTVVAFVVIQQIDNAFISPKIIEGRLGLHPVTTILAVLAGEEFFGITGMLVAVPVT
                     AVLKVILKRSIEAIV"
     gene            8029..>9627
                     /gene="alaS"
                     /locus_tag="PLA01_01600"
     CDS             8029..>9627
                     /gene="alaS"
                     /locus_tag="PLA01_01600"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPD00027.1"
                     /translation="MQKLGLNELRERYLSFFEGKGHLRLPSFSLIPQNDPSLLLINSG
                     MAPLKPYFTGKEEPPRRRVTTCQKCIRTPDIENVGKTARHGTFFEMLGNFSFGDYFKK
                     EAIPWAWEFFTKDLQIPEDLLWVSIYEEDDEAFEIWNKTVGLAPERIIRMGKEDNFWE
                     HGTGPCGPCSEIYFDRGPDKGCGSPDCKVGCDCDRFIEVWNLVFTQFNRDEEGNYTRL
                     PKPNIDTGMGLERLACVMQDVSNLFEVDTIRKVLDYICTLAGVRYGDSARTDISIRVI
                     TDHIRSTTMMISDGVIPSNEGRGYVLRRLLRRAARHGKLLGLDKPFLHDVAEVVINES
                     KQAYPELVERSENIRKVIRIEEEKFEETVDQGLVILNRYIEETRKRNEKVISGEIAFE
                     LHGTYGFPFDLTREIAEENGLGVDEEGFKVKMREHQVLAREDYRSKQGSAWGDDIYSS
                     LDVSKSTEFVGYNEKKANAEVLYIIKDNQVVDSASRGERVTIILDRTPFYAESGGQVG
                     DQGAIKAEGLLVKVDDCKKTNDGKY"
BASE COUNT         3058 a         1564 c         2080 g         2925 t
ORIGIN      
        1 ttcttacatt tctttacatt ctaaattatt aagtcatttt gcaaattcct aaaaacttat
       61 taagtatgta aatatcaact atagttgtaa tctaagagaa aaacatgtgc tttttattta
      121 tgaattatac aaattcttat tgtgaaggat tgtacttctt caaataaaaa tattctctga
      181 tactattatt ttattaaaca tttagaatta aatcaaccca aaagtgttaa caggatgcaa
      241 taaaatttga gaaaaaatat tttcggaaac tcaatttgtt tgacatcagc ttatgtttga
      301 aatattatat atatttgtac acacaaaatg attagcgatg gtaatagaga tataaatgtt
      361 ctaaaattga cattaaatta agataaaaat ataatattta atatggaggt tttaaattta
      421 ttttgtggga gggtgtcatg ggaaaactaa atttggttgt tgctgatacc gatgaggcct
      481 atgttgagaa catagtgaat tactttatga ctaaccattc tcagagattt caaataagtt
      541 cttttacgaa acatgagttt ttatatgatt ttctttcaga attaagagag attgacgttt
      601 tactggttag tcccgagatg tacaccgagt cattgcctaa agagggtata atagaaatct
      661 ttatattgac ttccggagaa ctgcctgaga aatgcaaagg attgaatcat atcgacaaaa
      721 accaaatagg ggatgggttc gtaaaagaga ttgccgacaa atatcctgaa gatgaagata
      781 atgctatcga agaacccatt gttgaagaac ctgtttcagt cgaacaagcc ataaaagaca
      841 ggaaaacaaa agttgtagcc atatattctc ctttaggagg tgtaggcaag actacaattg
      901 cagttagctc ttcgatatat tgcgggaaaa aaggacttaa tgtgttttat cttaattttg
      961 agtcttttca atctactccg ctttttttca attgtaaaaa tgaaaagagt tattcaaaaa
     1021 tattggagta tgtgaaggac aaaagggacg atcttcatag gatgattgag gcatcaaaac
     1081 aagtagatac tgatcacaat gtctattatt tgtctcctcc tgaaagcata cttgatattc
     1141 ttgaagccga accgaaggat tttaggagat taattaacga atttaagtcc atgggaatgt
     1201 atgatattgt aattgtggat atgtcaagcg aactgagcga cagaaatata gctttgcttg
     1261 aggcatgtga ccaagttgtt ctggtattgg ctcaggatgc catatccaat atcaaggcgg
     1321 aaagcttttc gcagcagctt gaggcacttc tgaaaagaaa gggactggat tttaccggga
     1381 agctgacttt ggtgctcaac aaatacaact ttcatatggc acttgaggtt gaaacagtgg
     1441 atataaatga agaatttata tcgatataca taccggtagt acctgggatg accgcgatta
     1501 agggaaatgc ccagcttgtt gatttgcagg gtgagtttgg tgccagcatt caggaactta
     1561 tggacaaaat catattgcac agttagttac ttttaaaaat acgatgagca agagaccgta
     1621 ggtttatgag ttctttatgt gaaaaataat ttatataaaa aatatgaata aaaggattat
     1681 ttcgaagaga aaatagatgt ggtaaactat agattctatt aggagcatta gaatggcggg
     1741 cagattggaa aaaacctatg gtaaggcatt taaatatctt ctctttgcta ttaatccgtt
     1801 taaaagattg gtcataaaaa ctgaggctgc gattcatcag ctcatcaatt tacaggcact
     1861 tgaaatttta aagaatgaca aatatattga tgctcattgc ctgttcagcg attacattga
     1921 ggatataaac aaaggggcag tatgggctga tttggatatc aagagtgtca accattttta
     1981 taaccctgtc aaaaaaaaag ggttatatgg caatgataat gcactgactc ttgcggtcag
     2041 gtattatggc aatgcaaagg aatattggta taaggataaa cccaacacct ctatgtttta
     2101 tctgggtgct gcagtacatt tggtacagga catgacagta ccgcatcatg caaatataag
     2161 gctgttggat aatcacagac agtatgaaaa ctttattcga aaaacatatt taaatacacc
     2221 taagttcttg gcggacagag ggggctatta cctgtccggc atagaagaat tcataacatg
     2281 caatgcccgc aatgcaatta aaatatatat gagacttaag gatattgaag acgcttcaaa
     2341 aaggtattat accatcgcaa aatttacaat tcctttggca caaaagacaa ctgcgggatg
     2401 ttttataaac ttctacaggg atatttcaaa aagaataatt tgattccaca agcaaaagct
     2461 catgggcatc gagcccattc gctttttggt catttcactt cgtgaaatcc cactaaaaaa
     2521 cagtcagagt aaacaccgca atgtgtttac tctgacatga aattatcttg acatgaaaat
     2581 ggtttacgaa atattgggtg cggttgtttt catcagctgc ataacttctc tgttgatttc
     2641 atccataggc ctgttgtttg ctatgtcatt caacagttgg cttattctat tcattacatc
     2701 ctgtgattcg cttgcaacca cgttgtttac attggggtca atttgcttga ctttttctat
     2761 gaccatgttt tttctggcat taggatcacc ctgggcattt gcaggactgt aaccgacgag
     2821 gcaggtgttg cctgttacca gtgcatttac cttgtctatt tcaggcattg catcaagttg
     2881 attggttata ttttctgatt tttgtttcat ttcctcagga ctctgaacac ccggtgtggg
     2941 tgcagccggt gccgcgggat ttcccatcgg agtagtgtcg attcttccta acaggccgct
     3001 tttagcattg ttgttcatgc cgttattgtt atttgcaccc ttattatcca ttgggtttaa
     3061 gtccatgttt aaaaacctgt ttggatttac actctgatta ttctgctggg tgttttgacc
     3121 ggttgcttcc ggttggttcc tcctggcagt gcctccaccc atattgcaac ccactagagg
     3181 caatgatgcg agtgtaagcc ctagtgttag agatgttgct tttaaaaagt ttttattcat
     3241 gaaaaccacc tccgtcatta tttttgacaa cactgttttt tttattagag ttaatattta
     3301 atactgaaaa taattattta caaatgaata agaaagttat tatcaaacca atttacggta
     3361 aagttgtttt gtgttccaca ttttgacgct tttgcatagt atacagtaaa ttcattattt
     3421 atatcaggag gtttttttgc atgtactata aagaatgtat gccttgcatg atgaatccaa
     3481 tggctatgcc ccaacattgc ggaccgatga tgccaatgat gcagcagcaa ttggagtcta
     3541 tgtatcctag aacatatcat atagtatatc cgcgtgttca atatcattgc gatatgttgg
     3601 atatgaatta tggatgtgct tattgtccta cacaagggca ggttgatgac atgtgtgaca
     3661 ggatatgcaa ggaagtagag aaggatattg aggcatgtat gaaggatgat atgagagagg
     3721 aaggagacag gcagattgga ttcggcggca gatttttatt aaatgccttt ataagaaccc
     3781 ttatcataag ggaacttctc ggaaggagaa gacgttttgg cccaccgttc tttggtttcc
     3841 cgggtttcgg tttttaagat taataaaagc acagtttttg tatagttata aaatgagccg
     3901 gagtaagctt ctccggctca ttaggttagt ccattacaat taaggttaat cctttacaat
     3961 ccttctgcac tcaaggtaga accgtttttc ttcagcttct cccatagaaa aagtttttct
     4021 tggaaggact ccatcaagta ttaccgtctt aaaaaggtca tgctttctca tggcaggaag
     4081 ataaaaacct atattgcctg cttttgagcc taatcttgtc actacatctt cgccgtgaat
     4141 ataatctatt tttgaatccc tgttgctttt gatgaaatca tcaaggaagc actggagcgt
     4201 ggcaacctcg agattgcatt ccgggtttct tattgtaaac accccaaagc ctgaagcatt
     4261 cacaaaacca aaattatggc acgatctgtc tttggtaagt tcggaaagct tatttttcat
     4321 ggattccaat gagtcaaatt tctcaaaggc cccgccgtat ttctcgaaat agtctgccat
     4381 atcctttaat attgcttcag gatcaatgtt gaaaaggacc ctgtgaatcg gttcaaaggt
     4441 aattccatca tcatgaacat tcacaacctc taccaatgca aatctggcag gatggttttc
     4501 cctctcttga gccgataaat tcttttttac gttttcccag tgtccctttg cagatgcaag
     4561 ggaatggttt ccgtctccca ctgcaaacaa aagcacaccc ttgtcgtccc tcaagccgta
     4621 tttatctctg aacgcctgcg gatcggcaag atcatttata gctttgaata taccgtccaa
     4681 agttttttga tcattgatct tatatccctt taaatgtccg ccgttcatca ttaactcaaa
     4741 gtcataaacc ttctcaaagg aatccagctt ttgctccagc ggttcaatga cggttttttg
     4801 aggatcatcg atgagaagca taacgtgggg aagttccaaa gaagcattct cccggatttt
     4861 tattctggga ggaagcctgt ctattactgt gttttcagtt gccctgataa gggtttgaga
     4921 tcctttggta tagtcatatt tttcaagatc gacagaaagg attaagcctt tcctggattg
     4981 aacgtgcgaa gtttttctgt caacaaatat catacaagga tcatcggaga caagagtacc
     5041 ttcactaata tattttttca tttcgctatt tattttttta attctttcag tttcgctgcc
     5101 gtctccaaga taaacctcag gaaaggttag gcggagagtt gacggattat cgccaacaat
     5161 ttcattaact ttattccagt attccggttc cgaagtgtac tggtcgcaag ctactaccga
     5221 ccataatgtc atgtcaacac cttttttagg aagcattatt gaaggaattt gaattcccaa
     5281 gtttttatca tatggaaata tatcagtcat tttgcacctt ccccataatt cgtaaatttc
     5341 aataagaatg attaataaaa agtgatttat tatagcaatt ttacaaacaa taattttata
     5401 tgaatttgca actcaagtca aactaaaagt tgtcaacggg tgtattaata aaagtgaaga
     5461 aaatatttca gaaactaaag tttcgcgagt ttagagcctg taaacactcc gtggggtcga
     5521 gcaaaactca cttcgttcag acaatgctct ccctttccac gtcgtattaa caggctacta
     5581 caacttgctt cacaatgttt cttccatatt ttcttcactt aaatattaat aaatagaggt
     5641 gaagaaaata ttccagaaac taaagtttcg caagtctagc cgcctgtaaa tacgataata
     5701 tcttcacatt tatttcatgt atttgctgcg tattttttat gttcatattg aaaataataa
     5761 acaatgaaaa aattccaaca tactaaacaa atcgaagaga aagcagcaat caaagcagca
     5821 aatttcgatg agtctggtta aacggagaga tgttctttgt taagatacag tgaggtgata
     5881 gggctgccgg tgatttgtgc aaccgatggt aagaggttgg gagtaataaa ggatattgtc
     5941 ttttgtccga aggataaaaa agttgttgcg tttttagtag aacgaaaagg ctacgaaatc
     6001 ttaaagaaag tagtttttat gaaggacatc caaagtatgg gcaaagatgc acttattatt
     6061 aatagcccga aggatataaa agatttaaaa aaagtgcagg acagcaatga gttaaaagaa
     6121 aaaggcgata tacttggttt aagagtgtgc acgaggtccg gtgaagacgt tggagttgca
     6181 aaagatgttt tgcttgattt tacgcacggt aaaattgatg gcattgaagt ttcgggagga
     6241 ttgattcagg atatactgga aggaagaaat attctgccgc tcattggcaa ggtggagttt
     6301 ggagaagagt atattctggt tgacagtgaa tcgatagaag aaatgatgga aacaggtgga
     6361 ggaataaaaa atataataga aggaaaaaaa agatttcagg aaaggggtga ttttgatgcg
     6421 aagtaacttt accagaggac tggtggtcgg aagcataatc ggtgcatcgg tgggagtggc
     6481 tatgaattcc ggtaaaatga tgaacaatag atccagaagg agaataaaaa acagaggatt
     6541 ggacataatg aggaagtcag gagcattaat aagcgatgta gtagaattat ttcgataaat
     6601 actttcaaat ccttgtaaag actggagaaa tccagtcctt actacataat cggcctttag
     6661 cacaagtaaa tcataggagc tgtgaaataa attataaggg gctcaataca tgattggaat
     6721 aaggagagta gtcagttaca tagtactatt tcttataggt gccgcaataa tcttatttgt
     6781 aacaacttat cgtgacaagc ttgtgaaaat attctttccc attgttctgg cagggattct
     6841 ggcctacatt attcgtcctc tggtattgaa gcttgaaaag aggaacatat ccagaacaaa
     6901 aggtatactg ttgatttatt tggtttcagg tatagtactt attgtatctg taatattcat
     6961 tgtaccggtg ctggtaaaca acacccgtga gtttattaac acatttcctg aattgacact
     7021 ggagtacaag aacaatttta acaatgtggt taaaatgata gatacgagta attggccccc
     7081 ggaaataaaa aatgctgtct tcagggagat aaacaatacg gctggtatat tggaaaccaa
     7141 gattttgaat gcattgagaa atgcattgtt tggttcaata aatgcggctg cacgcatagg
     7201 cgacttcata atagcaatga taattgcgta ctacctgata aaggatgcag agttttttaa
     7261 gagaaatttc ctttcccttg tgccgaaagg atggaggaat aatgctattg ccacatgcag
     7321 agaaataaat gatatacttt ccagctttat acatgggcaa atattaacgg cggttattat
     7381 cggaatcatg gaagcaacag tacttcttat aattggcata aaatatgcac cgatacttgg
     7441 aataattgga ggtatagcaa atatagtacc ttattttgga ccctttattg gggcaatacc
     7501 tgcagtggct gtggcattta tcgattcgcc tgtaaaagtc atttggactg ttgttgcctt
     7561 tgttgtcata caacagattg ataatgcatt tatttcacca aagattattg aagggaggct
     7621 gggacttcat cctgttacga ctattcttgc ggtattggca ggagaagaat ttttcggtat
     7681 aaccggtatg ttggtagcgg ttcctgtgac tgctgtttta aaggtaatcc ttaagaggtc
     7741 aatagaggct attgtatagt tcttgacact cagacaatac ttatatataa tcttactgaa
     7801 taatgcctct caacagaatt gactggaggg gtttcatgta cgcaaaaatg gatttggaca
     7861 taataaaaga ctgtgaaaat attctttata taaaaatttt atttaaaaag agttgaagaa
     7921 ccgcttcctg aataaagggg aaacgctctc gtacctaaaa atactaggag gagagttttt
     7981 atattatagg attaagtaat gagtaaaaac cgggagaggg agaataaaat gcagaagtta
     8041 ggattaaatg aactgagaga aagatattta agtttttttg agggcaaggg gcacttgagg
     8101 cttccaagtt tttcattgat tccgcaaaat gaccccagtc ttcttttgat aaactcggga
     8161 atggctccct taaagccgta ttttacgggg aaggaagaac cccccagaag aagagttacc
     8221 acatgccaga agtgcatcag gacaccggat atcgaaaatg tggggaagac tgcaagacac
     8281 ggcacttttt ttgaaatgct tgggaatttc tcatttggtg attatttcaa aaaggaagct
     8341 attccatggg catgggaatt cttcacaaag gaccttcaga ttcctgagga cctcctctgg
     8401 gtatctatat atgaagagga tgacgaggcc tttgaaatat ggaataagac tgtaggtttg
     8461 gcaccggaaa gaattataag gatgggaaag gaagacaact tctgggagca tggtacgggt
     8521 ccctgcggtc cctgttcgga aatctatttt gaccgggggc cggataaagg atgtggaagc
     8581 ccagattgta aggtgggctg cgattgtgac aggtttatag aggtgtggaa cctggtgttt
     8641 acacagttta acagggatga ggaaggaaac tatacacgtc ttccaaaacc gaatatagat
     8701 acaggaatgg ggcttgagag gcttgcatgt gtgatgcagg atgtgagcaa tctttttgag
     8761 gtggacacaa tcagaaaagt gctggactat atatgtacat tggccggtgt caggtatgga
     8821 gactctgcca ggacggatat ttccataagg gtcataaccg accatatcag aagtacaaca
     8881 atgatgatat cggatggagt tataccttcg aatgagggca ggggttatgt attaagaagg
     8941 cttcttagaa gggctgcaag gcatggaaag ctgctgggcc tggacaagcc gtttttgcat
     9001 gatgttgcag aagtggtcat aaatgagtca aaacaggcat atcctgaact tgttgagagg
     9061 tctgaaaaca taaggaaggt tataagaata gaggaagaaa agtttgaaga aactgtggat
     9121 caaggacttg ttatattaaa ccggtacatt gaggaaacca gaaaaagaaa tgagaaggtc
     9181 atcagcggcg agatagcttt cgaacttcat ggcacttatg gttttccctt tgatttgaca
     9241 agagaaatag ccgaggaaaa cggacttggc gtggatgaag agggctttaa ggttaaaatg
     9301 agggagcatc aagtgcttgc gagagaagac tatcgaagca agcagggttc tgcatgggga
     9361 gatgatatct actcaagcct tgatgtaagt aaaagtactg agtttgtagg ttacaatgag
     9421 aaaaaggcaa atgccgaagt actctatata ataaaggata accaagtggt agatagtgct
     9481 tcccgcggtg agagggtcac gattattctt gaccggactc cgttttatgc tgaaagcggc
     9541 ggacaggttg gagatcaggg tgctattaag gcagaaggat tactcgtaaa agttgacgac
     9601 tgtaaaaaga ctaatgacgg aaaatac
//
LOCUS       DAOOJC010000030         9437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_6418_length_9437_cov_4.151140, whole genome shotgun sequence.
ACCESSION   DAOOJC010000030 DAOOJC010000000
VERSION     DAOOJC010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9437
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6418_length_9437_cov_4.151140"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..2332)
                     /locus_tag="PLA01_01605"
     CDS             complement(143..2332)
                     /locus_tag="PLA01_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HPD00028.1"
                     /translation="MRNPLRRFVGVLLSIFIVLGTSTVNGAQTNPWNAYEKFIPAETP
                     VLKRHLRGAWISTVVNLDWPSVETVKIGNTVERITKSKEELTEILDRAVELKLNAVFF
                     QVSPEGDAFYKSNIVPWSRYLTGTFGKDPGFDPLAFAIEEAHKRNLEIHAWFNPYRVS
                     MNTNSSTISSLNIEKSVYKEHPEWVKKSMNRFVVDPGIPEARKWVIDRVMEVVENYDV
                     DGVHFDDYFYYEEYEGELKDQDTFNKYNNGKFKNLSDFRRNNTYLLIKELSGKIRSAK
                     QWVKFGVSPAGIWANKKDGHPDGSNTNSSFTNYDKSFADTKKWVVEELIDYIAPQIYF
                     TFANPRAPYGELASWWSDVCRGKNVHLYIGQAFYKINDDTDEYFKGSNAVPELSRQLK
                     FNMAKPEIMGTIMFRFKNFEDSGKQQAVGAVKNNLWTTNSLVPVMPWKGGSTPDTPIL
                     GKLESLPKGVKLSWTDNDPDTAYFAIYRFNTGEKTDIVSDQSALKLLTTIRKNHSGVQ
                     EFVDYEIGNADSVYYVVTALDRLHNESEGLAVSTNQSEYFPDVGMKYSWAVDAIDMLY
                     ERGVIKGDENGMYNPGVNTKRGDFTIMIVKALGFEAEFTDNFSDVKPGSYYYDAIGVA
                     RELGIVKGDGNNFNPDSNITREDMMVIVVNALNKAGAMIEKADEKYLDNYSDAKNISN
                     YAREAVALLTAKGIVNGFEGRINPKGMATRAEIAVVFANVLNKIEFM"
     gene            2940..4922
                     /locus_tag="PLA01_01610"
     CDS             2940..4922
                     /locus_tag="PLA01_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPD00029.1"
                     /translation="MGQKHSKISLLSVLTIIAMIISCLFGFIAVNADDTTENTALEGL
                     SIHYMDGSLDERYQSMRPYIIIYNNSGESVDMADLKVRYYYTKEGATEETLLCFYTAV
                     GSANIFAEFHPELGYAEIGFTSGAGEIKDGGNSGQMQLVLKKISNGYYDQTNDYSYDP
                     SFTDYAEYDKMTLYYKDTLVWGIEGPPPPPEPAPPPNNDDWLHVEGNLIKDLKGNTVY
                     LTGINWFGFETDGANGFYGLNKCNLEDSLDLMANLGFNLLRIPISAEIILEWKSGKYV
                     DTSFVNTYENPRLDGLSSLDILDFTINHMKKNGMKVMFDMHGATKDSYQDNLWYSKDM
                     TMDEFIEAWKWLTERYKDDDTVIAMDLKNEPHGKFSGPSIAKWDDSEDPNNWKKAAEI
                     IAEEVLEINPNLLIVVEGVEAYPMDGYDYTNCGEFTTYCNWWGGNLRGVAHHPVTLSV
                     PDKLVYSVHDYGPDIYVQPWFKKPFDINTLYEECWYPNWYYIVDQNIAPMLIGEWGGK
                     LKNDDNRKWLECLGSFIAENKLHHTFWAFNPNSADTGGLMLEDWKTVDEDKYAIIEPT
                     LWKKGLDHVIPLGGITPGTIKYGDVNGDFEIDSTDLTLLKRYVLRKITEFPSPYGLQA
                     ADLNGDKDINSTDVTLIKRYILKTLTVFPVEEGLEK"
     gene            complement(5005..5859)
                     /locus_tag="PLA01_01615"
     CDS             complement(5005..5859)
                     /locus_tag="PLA01_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPD00030.1"
                     /translation="MLKKMTIGITSVMLFCMVFYQCVFAAENLLQNPSFEDTNNNMPV
                     GWYTWVWDYKPGTVEFFVEQEGSRSGQNYVTIENKEGRDSRYYQEVEVNSNSCYKFSA
                     WIKTKNVSEKALGANLSLEGITTHSSDVKGTVDEWQYAELYIRTGEGVNTLKLSLGLG
                     GYGNLNIGKACFDDAVLEEVDSIPDGVNYALVENPDAVQKPSDAEQKPDPENTKGKYT
                     WVWLIISLAVLAIILFYYFISGKLKLTKTGDSEKEQKDTEYTEKGKNVTGYIEEDKKD
                     IDYTEEDQ"
     gene            complement(5918..7207)
                     /locus_tag="PLA01_01620"
     CDS             complement(5918..7207)
                     /locus_tag="PLA01_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPD00031.1"
                     /translation="MNICVVGAGATGLVAANELVKKGHKVYVFEAEEQHGGLVNTITV
                     GNEKLEVFYHHIFTNDVEIIKLIEELDLSSELMWLEPKNSIYINRKLYPFTSPVDLLL
                     FKELSFIDRIRMGLLVFKARFIKDWNQLENMSSKDWIIKNAGKSVYEKVWGPLLSSKF
                     DYDADDISGTWIWNKFKLRGSTRGKNLNKELLGYMKGSFGVIYDKLAEKIINAGGEIY
                     YSSPVDRIEPKEDKTLDIHCNGNIYNFDRVIVTTSPEILNKMDIPFPEEYNEKLSKVK
                     YKANICMILELSEKLSDYYWTTIAEKDFPFVLLIEHTNLVIENDYKSHIIYLSRYLDR
                     KNEIYSLSNDEIQSEFIKYLKIMFPDWDESKIRRVHINRTSYAQPVVVQQYSKILPQI
                     DTPVENLFLASMAQIYPEDRGQNYSVRLGKQVADMIK"
     gene            complement(7227..8597)
                     /locus_tag="PLA01_01625"
     CDS             complement(7227..8597)
                     /locus_tag="PLA01_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00032.1"
                     /translation="MDKTNPKNTKKRSWFSNVEKPIPLEALLIAGAAVILIRLLLTAI
                     PSYQVDMGGYRAWSNYLAEKGPKGLYEDFHIVYAPAYMYLLWITGIIAKAFSVNAATH
                     AYLIKLWAVISELIGAYLIYKIGKKYGKERLGFILGVVYALNPGVFFNSSIWGQFDSI
                     SATLLVGMIYAFSVNRKMTAVVLYSLAILTKPQSALLTPLGILFYKDLFDFSKITKQK
                     IIKSIKDTLVAVCIGFSCYFIVIYPFYYHMDLYEKMKSTSVVKDFIAETVDYFCWMPN
                     LYLTSVEDYPYATANAFNLWTLLGGQPMDDSKILYILSFKTWGTILFLVSIGIAFAYL
                     LKKRKSDFAMYFASFFIMASAFTFMTRMHERYLLPAIIFLTVSVLWKKWMAIPLAVLS
                     ICVTANHWYIYHLSWKEVFWLSNYDPVAMPFAFLTVLLVVFSGGLIVKQILPSKKIEG
                     EECYPE"
     gene            complement(8722..>9437)
                     /locus_tag="PLA01_01630"
     CDS             complement(8722..>9437)
                     /locus_tag="PLA01_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00033.1"
                     /translation="MVFAFLVLVFASMIYTSNNDSPTPRGTRALMLAFAAPIIFASTV
                     KVTRTVDINHKYVVIGSILINIFIAAFIFFLFKVKKPLAIIAAVSVSIVITVTGFVDV
                     ITLYNLNNISVDVRCDDPLLVKVKNETGKDEIFLTDDLHLHPILLAGRKIFCGWPYFT
                     WSAGYEWGLRDEIRKEIFTAVDESVLKELVRENNISYIVIDNAIRDSDNYTVNEQLIK
                     DTFEVFYDDGHDIVIYKTY"
BASE COUNT         2881 a         2015 c         1620 g         2921 t
ORIGIN      
        1 acccgtggct agactttcac tagctagatt agtgccatgc ctggcacaca gtaaagaaga
       61 atgcttcaca atattttgga aacattcttc tttagctaat atacaccggc tatttaacaa
      121 aatccattat aaccgtaaag gattacataa actctatctt gttcagcaca ttggcaaaaa
      181 ccactgcgat ttctgcccgg gtagccatac ctttaggatt tatccttcct tcaaaaccgt
      241 tgacgattcc cttcgctgta aggagtgcca ccgcttctct ggcataatta ctgatattct
      301 tagcatcgct gtagttatca aggtattttt cgtcagcctt ttcaatcatt gcacccgcct
      361 tattaagtgc attcaccaca ataaccatca tatcttccct ggtaatattg gaatcgggat
      421 taaaattatt tccgtctcct ttaactatcc ccagttcccg ggcaacacct attgcatcat
      481 aataatacga acccggtttc acatcggaaa aattatcggt gaactcagcc tcaaatccca
      541 gtgcctttac gatcataata gtaaagtcac ctctttttgt gttaacaccg ggattgtaca
      601 tgccgttctc gtctcccttt ataactcctc tttcatagag catatcaatg gcatcaacag
      661 cccaggagta tttcataccc acatcgggaa aatactccga ctggtttgtg cttacagcga
      721 gtccttcgct ttcgttgtgg agcctgtcca aggcagttac gacataataa acactgtcgg
      781 cattgccaat ttcataatca acaaattcct gtactccgct atggtttttt cttatagtag
      841 tcaaaagttt taaagcactt tgatctgata caatatccgt cttttcccct gtattaaagc
      901 ggtagatggc aaagtaagca gtatcggggt cgttatccgt ccaagatagc ttgactccct
      961 tcggaaggct ctctaatttg cccagaattg gtgtatcggg agtacttccg cccttccacg
     1021 gcattacggg aaccagtgaa tttgttgtcc agagattgtt cttaacagct cccaccgcct
     1081 gctgcttacc ggagtcttca aaatttttga acctgaacat aatcgtcccc ataatctctg
     1141 gctttgccat attgaatttc agttgtcttg aaagctcagg caccgcattg gaacccttaa
     1201 aatactcgtc ggtatcatca ttaattttat agaaagcctg acctatataa agatgtacat
     1261 ttttccccct gcaaacatcg gaccaccaag aagctagctc tccatatggt gctcttgggt
     1321 tggcaaaggt aaaataaatt tggggagcaa tataatctat aagctcttct acaacccact
     1381 ttttagtatc ggcaaaggac ttgtcatagt ttgtaaagct tgagtttgtg tttgaaccgt
     1441 caggatgccc gtctttttta ttagcccata ttcctgccgg gctcacaccg aacttaaccc
     1501 attgctttgc agaccttatc tttccggaaa gctccttaat gagaaggtaa gtattgtttc
     1561 ttctgaaatc actcaaattt ttaaactttc cgttgttgta tttattaaag gtgtcctgat
     1621 ccttcaattc cccttcatat tcttcgtagt aaaaatagtc atcaaaatgt accccgtcaa
     1681 catcatagtt ttcgactacc tccatgaccc gatcaattac ccattttctt gcctcaggaa
     1741 tccccgggtc cacaacaaac ctgttcatgg atttcttaac ccattcgggg tgctccttat
     1801 aaacactttt ttcaatattc aaagacgata tggttgaaga atttgtattc attgataccc
     1861 tgtacggatt aaaccatgcg tggatttcca gattcctttt atgagcttcc tcaatggcaa
     1921 acgccaatgg gtcgaaaccg ggatcttttc caaaagttcc tgtaagataa cgggaccacg
     1981 gaacaatatt ggatttgtaa aatgcatccc cctccggact gacctggaag aaaaccgcat
     2041 taagcttcag ttccactgcc ctgtccaaaa tttctgtcag ttcttccttg ctctttgtta
     2101 tacgttccac agtatttcct attttaacgg tttcaacaga aggccaatcc aggtttacaa
     2161 cagtactaat ccaggctccc ctcaaatgcc tttttaaaac aggcgtttct gccggtatga
     2221 atttttcata ggcattccat ggatttgttt gtgccccatt aaccgttgag gttcccaaga
     2281 ctataaaaat agacagcaaa acacccacaa atcttctcaa aggatttctc atatgtgtta
     2341 acccccaaag tcacaaaaat tatattctag gcttataatc aatatgttaa gttctctgtt
     2401 gtaattagtc aagctgtaaa tattttcttc actttttcta ctgcacccgc atgggaaaga
     2461 tctacctcta gacttgcgta actttagttt ctggaatatt tttctttaca ttcatctaat
     2521 acacccgcat gagatggacc tacattattt aattgaagaa aaatatgaaa gaaacattgt
     2581 gaagcaagtt gtagagactg tttatacgac gtggaaaggg agagcattgt ctgaacgaag
     2641 tgagttttgc tcgaccccac ggagtattta cagtctctag acttgcgtaa ctttagtttc
     2701 tggaatattt ttcttcacat ttttaaatcc acccaaattt tatagataga ttttgaaaac
     2761 attatattca tgatataatt aaaatagtac atttaattta tatttatata gaattggttc
     2821 gaatttgaga taaatatata aaactattat gtttatatat aattttttct acaagggggg
     2881 aagtaaattt taatcatttt atttttcttt aattaataag tgagggaggt taagttttaa
     2941 tgggtcaaaa gcattccaag ataagcctgt tatctgtatt aacaattata gccatgatta
     3001 tttcctgcct ttttggcttc attgccgtca atgcagatga caccaccgaa aatactgctt
     3061 tggaaggtct atcaatccat tatatggatg gttcattgga tgaaagatat cagagcatgc
     3121 gtccttatat aattatttac aacaacagcg gtgaaagtgt agatatggca gacctcaaag
     3181 taaggtatta ttatacaaaa gaaggtgcca ccgaggaaac ccttttatgt ttttatactg
     3241 cagtaggatc cgcaaacata tttgcagaat tccatcccga actgggatat gcagagattg
     3301 gttttaccag cggagccgga gaaattaagg atggtggcaa cagcggtcag atgcagttgg
     3361 tactgaaaaa aatatccaac ggctattacg atcaaaccaa tgattattct tatgacccca
     3421 gtttcaccga ttatgcggaa tatgacaaaa tgacgctcta ctataaggac acgctggtat
     3481 ggggaataga gggaccacca ccaccgccgg aaccggcacc gccgccaaac aatgacgact
     3541 ggcttcatgt ggaaggaaat ttaattaagg atttaaaggg gaacaccgtc taccttacag
     3601 gtatcaactg gtttgggttt gaaactgacg gagcaaatgg attttacggt ctcaacaaat
     3661 gcaatctgga agactctctg gatttaatgg caaacctggg tttcaatctt ttaagaatcc
     3721 ctatcagtgc tgaaatcatt cttgaatgga aaagcggaaa atatgtagat acttcctttg
     3781 taaacacata tgagaaccct cgccttgacg gtctgagcag tctcgatata ctggacttta
     3841 caataaatca tatgaaaaaa aacggtatga aggttatgtt tgacatgcat ggtgctacta
     3901 aggattcata tcaggacaac ctctggtaca gtaaagatat gactatggat gaatttatcg
     3961 aggcttggaa atggcttacg gaaaggtaca aggatgatga tacggttatt gctatggacc
     4021 ttaaaaatga gcctcacgga aagttctcag gtccgagtat tgccaaatgg gatgattcgg
     4081 aggatccgaa caattggaaa aaggcagcag agattatcgc cgaagaagta ctcgaaatta
     4141 atccgaacct tttgattgtc gtagaaggtg ttgaggctta cccgatggat ggatacgatt
     4201 acaccaactg cggtgagttt actacatact gcaactggtg gggcggaaat ctaaggggtg
     4261 tcgctcatca ccctgtcacc ctgtctgttc cggacaaact tgtatattct gtacatgatt
     4321 acggaccgga catatatgtg cagccatggt ttaagaaacc ttttgacatt aatactcttt
     4381 atgaagaatg ctggtacccg aactggtatt atattgtcga tcaaaatatt gcacctatgc
     4441 taatcggaga atggggcggc aagctaaaaa acgatgacaa ccgcaaatgg cttgaatgtt
     4501 tggggagctt tattgctgag aataaactgc atcatacctt ctgggccttt aatccaaatt
     4561 cggcagatac gggcggtttg atgcttgagg attggaaaac cgtggatgaa gataagtatg
     4621 caatcatcga accaactttg tggaaaaaag ggctggacca cgtaatacca ttaggaggaa
     4681 ttacaccggg aaccatcaaa tacggtgatg ttaacggtga cttcgagata gactccaccg
     4741 atcttacatt acttaaacgt tatgttctta gaaaaatcac cgaattccct tctccttacg
     4801 gattacaagc tgctgacctg aacggagaca aagatataaa ctccaccgac gttaccttaa
     4861 taaaaagata tattctaaaa actttaaccg tatttcctgt agaagaggga ttagaaaaat
     4921 aaccaatgag aattcaatgt aaaaacatat aaatctttac aaaagcaatg ggggccattg
     4981 agcccccatt gctttttgga ggttttattg atcttcttca gtatagtcaa tatctttctt
     5041 gtcttcttca atatatccag taacgttctt gcctttttca gtatattcag tatctttttg
     5101 ttctttttca ctatctccgg tttttgttaa tttaagtttt ccggatataa agtaataaaa
     5161 taatatgatt gctaaaacag ccaatgaaat tataagccag acccaggtgt acttgccttt
     5221 cgtattctca ggatcaggtt tttgttcagc atccgaaggt ttctgtacag catcaggatt
     5281 ttcaaccaat gcataattta caccatcagg tatgctgtct acttcctcca atactgcgtc
     5341 atcaaagcat gcctttccga tattcagatt gccatatccc cccagaccta aagataactt
     5401 taatgtatta acaccttcac cggtcctaat atataactca gcatattgcc attcatcaac
     5461 tgtacctttg acatcacttg aatgcgtagt aattccttcc aatgacagat tggcacccaa
     5521 tgccttctca ctaacattct ttgttttaat ccaggcagaa aacttgtagc atgagttgga
     5581 attgacctct acttcctgat agtaccgtga atccctgcct tctttattct caatggtaac
     5641 gtaattctgt ccgctgcggg aaccttcctg ttccacaaaa aactccacgg tacccggctt
     5701 gtagtcccaa acccatgtat accaacctac gggcatatta ttgtttgtat cttcaaaaga
     5761 tggattctgc aataggtttt ctgctgcaaa aacacactga taaaacacca tacaaaatag
     5821 catcacagaa gttataccga ttgtcatctt cttcaacatt ataatcttgc ctccaatata
     5881 atatattcaa aaataaaata tataaaatta tatcctgtta tttaatcata tccgctacct
     5941 gttttccgag cctgacagaa taattctgcc ccctgtcctc gggatatatt tgggccatac
     6001 tggctaaaaa caagttctct actggagtat cgatttgggg taaaatcttt gaatattgtt
     6061 gcacaacaac gggttgtgca tagcttgtcc tgttaatgtg cactcttctt atctttgact
     6121 catcccaatc gggaaacata attttcaagt atttaataaa ttcgctctga atttcatcat
     6181 tgcttaacga ataaatctca ttttttctat cgagatacct ggaaagataa ataatatgtg
     6241 atttataatc attctcgata accaagttgg tatgctctat taacagcaca aaaggaaaat
     6301 ccttctctgc aattgtagtc caataataat ccgataattt ctcggaaagc tccaaaatca
     6361 tgcaaatatt tgctttgtac tttacttttg agagcttttc gttgtattct tccgggaagg
     6421 gaatatccat tttatttaga atctccggcg aagtagtcac aataaccctg tcaaaattat
     6481 atatatttcc attgcaatgg atatccaacg ttttatcttc tttgggttca atcctgtcca
     6541 caggacttga gtaatatatt tcaccaccgg cattgattat tttctctgcc aatttgtcgt
     6601 atattactcc gaaacttcct ttcatatatc ccaatagttc cttgttaagg ttttttcctc
     6661 ttgttgaacc cctaagctta aatttgttcc atatccaggt gcccgaaata tcatcggcat
     6721 cgtaatcaaa ctttgaacta agcaaaggtc cccagacttt ttcatataca ctcttacccg
     6781 cattttttat tatccagtct ttagagctca tattctccaa ttgattccaa tcttttataa
     6841 acctcgcttt aaaaacaagc aagcccatcc ttatcctatc aataaacgaa agctccttaa
     6901 aaagtaacaa atccacagga gaagtaaaag gataaagttt tcggttaata tatatggagt
     6961 tcttcggttc gagccacata agctcagaag acaaatccaa ctcttcaatc agcttaatta
     7021 tttctacatc attcgtaaat atatggtggt agaacacttc caatttttca ttgccaaccg
     7081 ttatagtatt taccaatcct ccatgctgtt cctcagcttc aaacacataa actttatgac
     7141 ctttcttaac aagctcattt gcagcaacaa gccctgtagc tcctgctccg acaacacata
     7201 tattcataat cccttgtctc tcctttttat tctggataac attcttcacc ctcaattttt
     7261 ttggaaggta atatctgttt tacaatcaat ccccctgaga acacaaccaa caataccgtt
     7321 aaaaaggcaa agggcatggc aaccggatca taattactca gccaaaaaac ttctttccat
     7381 gacaagtgat atatatacca gtggtttgca gtgacacata tactcaatac tgccaagggt
     7441 atagccatcc acttcttcca caggacactg accgtaagga aaattatagc cggaagcaag
     7501 tatctttcat gcattctggt catgaaggta aaagcacttg ccataataaa gaaagaagca
     7561 aaatacattg caaaatcgct tttccttttt ttcagcaaat acgcaaaagc tatacctata
     7621 gaaactaaaa acaatatagt tccccaagtt ttaaaggata atatatagag tatttttgag
     7681 tcgtccatcg gttgtcctcc caatagcgtc cacaggttaa aagcattggc agtagcatac
     7741 ggatagtcct caacactcgt taagtataga ttgggcatcc agcaaaaata atccactgtc
     7801 tctgctataa aatctttaac gacggacgtg ctcttcattt tttcgtaaag gtccatgtga
     7861 taataaaagg gataaattac aataaaatag cacgagaacc ctatgcagac agccaccaat
     7921 gtatccttga tactcttaat aatcttttgt ttggtaatct ttgaaaaatc aaataaatct
     7981 ttgtagaata atatacccaa cggagtaaga agtgcacttt ggggcttggt cagtatagca
     8041 agagaataca atactaccgc agtcattttt cgattcacac taaaggcata tatcataccc
     8101 acaagcaaag tcgcagatat tgaatcgaat tgtccccaaa tcgaagaatt gaagaatact
     8161 ccgggattaa gtgcataaac tactcctaaa ataaatccaa gtctctcttt cccgtatttt
     8221 ttccctattt tataaataag gtatgcccct atcaattctg aaataacggc ccatagtttt
     8281 atcaaataag catgggtagc cgcattaact gaaaatgctt tggctattat ccccgtaatc
     8341 catagtaaat acatgtaagc cggtgcatat acgatatgga aatcctcata aagacccttc
     8401 ggaccctttt cggcaagata attactccac gccctatatc ctcccatatc cacctgatag
     8461 ctcggaattg cagtcagaag cagtcttatt aatattacgg ccgctccggc aattaataac
     8521 gcttcgaggg gtatcggttt ttccacattg gaaaaccaac ttcttttttt tgtgtttttc
     8581 ggattagttt tatccatagc cagaaaaccc accttactct tatagtaatt agaatcatta
     8641 atccatagca tggcagcatt caaaccgcat aaacctttag caaaccaagc ttataccctt
     8701 accaaaaaaa ctataacaaa tctaatatgt tttgtaaata acaatatcat gaccatcatc
     8761 ataaaaaacc tcgaaggtgt cttttatgag ttgttcattc acagtataat tgtcggaatc
     8821 tctgattgcg ttgtctatca ctatatagct aatgttgttc tctcttacca gttccttgag
     8881 cacgctttcg tcaactgcag taaaaatttc ttttcttatt tcatccctaa gaccccattc
     8941 atagcctgca gaccacgtga aatacggcca gccgcagaat attttccttc ctgctaatag
     9001 tatcggatga agatgcaaat catccgtcag gaaaatttca tcttttccgg tctcattttt
     9061 taccttgaca agtaaaggat cgtcacagcg gacatcgaca gagatattgt taaggttgta
     9121 aagtgttatt acgtccacaa aacctgtaac ggtaattact atggaaacag acacggctgc
     9181 aatgatggca agaggttttt ttactttgaa aagaaagaat atgaaagctg ctataaatat
     9241 atttataagt atggaaccaa taaccacata tttatgattg atatcaacag tcctagttac
     9301 tttgacagta cttgcaaaaa taatcggagc agcgaatgcc agcatcagtg cccttgttcc
     9361 ccgtggagtc ggagaatcat tattggacgt atatatcatg ctggcaaata ctaacactaa
     9421 aaaagcaaat accattg
//
LOCUS       DAOOJC010000031         9221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_6585_length_9221_cov_3.042003, whole genome shotgun sequence.
ACCESSION   DAOOJC010000031 DAOOJC010000000
VERSION     DAOOJC010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 9221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9221
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6585_length_9221_cov_3.042003"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLA01_01635"
     CDS             complement(<1..695)
                     /locus_tag="PLA01_01635"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPD00034.1"
                     /translation="MANFPDMPSNKPVIGIPLGDPAGIGPEIVVKALREKSIYRFIRP
                     VVIGSRDAVNKALKICGLDSRINKVTDASEGIYNEGIVNLIDYEDVDVSGVITGKVQA
                     IGGEAAYSYIKHSVELAQKGVIDAVATTPINKESLKMAGINCIGHTEIFSELTNTRDP
                     LTMFEVRDLRVFFLTRHVSLSQACGLVNKEKVLSYIQRCTRALELLGIKGGTMAVAGL
                     NPHSGEHGLFGSE"
     gene            complement(870..1130)
                     /locus_tag="PLA01_01640"
     CDS             complement(870..1130)
                     /locus_tag="PLA01_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HPD00035.1"
                     /translation="MEVLKVSAQSQPKMVAGALAAILRGKSTAEIQAVGAAAVNQAIK
                     AIAITRGFVAPNGIDIITVPAFSSISIDGEERTAIKFLVEPR"
     gene            complement(1289..2305)
                     /locus_tag="PLA01_01645"
     CDS             complement(1289..2305)
                     /locus_tag="PLA01_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD00036.1"
                     /translation="METKHIIIPIFIPHKGCPFDCIYCNQKKISGQIDEMTEEKMVSE
                     IESCLSTAGNDAYVEMAFYGGSFTGIEKSEQLKFLKIANEYIKKDKIKAIRLSTRPDY
                     INGDILDYLKEYKVRTIELGVQSLDNEVLEKSCRGHSQEVVFDSSKLIKEYGFVLGIQ
                     TMIGLPGDTDEKALETAKKVVGLKPDLLRIYPTLVIKDTYLEKMYNKGEYTPLNLEDA
                     VALCAKLLRIYRENNINVIRIGLQPTESINGNGDVVAGPFHPAIRQLVESKLALSIIE
                     EIIEQNNLSKKSSLTIGTSRKRISDVVGQKRSNVNYLKEKYGYESVMVRECSDVHEIN
                     DIKY"
     gene            complement(2385..3095)
                     /gene="rnc"
                     /locus_tag="PLA01_01650"
     CDS             complement(2385..3095)
                     /gene="rnc"
                     /locus_tag="PLA01_01650"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPD00037.1"
                     /translation="MPDLKALSVKTDEFEKIIDYCFKDKKKLVLALTHSSFANECRNE
                     KMVSNERLEFLGDAVLEMVVSEHIYLNATDFTEGEMTKFRANVVCERSLAKCAKRINI
                     GNYLLLGKGEENSGGRKRTSILSDAVEALIGAIYLDGGFESAKAFILRQLEDSINGAI
                     KGTIYTDYKTQLQEYVQKTSDHTISYEIIEEKGPDHSKMFVSQVKIDEKTVGIGMGRS
                     KKEAEQMAAKSSLEKIMI"
     gene            complement(3296..4528)
                     /gene="fabF"
                     /locus_tag="PLA01_01655"
     CDS             complement(3296..4528)
                     /gene="fabF"
                     /locus_tag="PLA01_01655"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPD00038.1"
                     /translation="MKRRVVITGMGVVSALGTDINEFWNSIKEGKCGITTVSKFDVSC
                     MPTKVAAEIKDFDPTRFIDKKEAKRMDIYTQYALCAAKMAYNMSGIDSEKIDPYRFGV
                     IVGSGIGGITTFEEQFRVYIDKGPGRVSPFFIPMMISNMATGRIAIEFGAKGFNECVV
                     TACATSTNAIGDAFKVIQRGDADVIITGGAEASITPMAFAGFCSMKATSTTEDPNLAC
                     RPFDAERNGFVMGEGAGILILEDLEHAKERGADIIAEIVGYGSTNDAYHITAPAPEGE
                     GGAKCMQMAINDAGIKPEEVGYINAHGTSTEYNDKFETAAIKTVFGEHAGKLPVSSTK
                     SMTGHLLGAAGAVECIITALTLKEGYLPPTINYRNPDPECDLDYVPNVGRKADITYAA
                     SNSLGFGGHNATVVLKKF"
     gene            complement(4663..4890)
                     /gene="acpP"
                     /locus_tag="PLA01_01660"
     CDS             complement(4663..4890)
                     /gene="acpP"
                     /locus_tag="PLA01_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPD00039.1"
                     /translation="MVFEKVKKIIAEQLGVDEDEITMESSFIDDLGADSLDIVELIMA
                     LEEEFDIEIPDSEAEKISVVGDVVEYIKNNS"
     gene            complement(4984..5727)
                     /gene="fabG"
                     /locus_tag="PLA01_01665"
     CDS             complement(4984..5727)
                     /gene="fabG"
                     /locus_tag="PLA01_01665"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003495675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPD00040.1"
                     /translation="MKLKGKTAIITGSGRGLGKAIAFKLGSMGANIVLNDIPASDSID
                     QTAEEFKAAGINVAVTKGDVRNFEDAEAMVKTAVEAFGSVDILVNNAGITRDNLILRM
                     TEQEWDDVLNINLKGAFLCTKAASKVMLKQKSGKIINIASVAGVMGNVGQANYSAAKA
                     GMIGLTKSTAKELASRGINCNAVAPGVIMSKMTDVLPDNVKENYLNNIPMKRFGTPED
                     VADVVSFLASDDSDYVTGQVIHIDGGLVM"
     gene            complement(5818..6753)
                     /gene="fabD"
                     /locus_tag="PLA01_01670"
     CDS             complement(5818..6753)
                     /gene="fabD"
                     /locus_tag="PLA01_01670"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPD00041.1"
                     /translation="MGKLAFLFSGQGAQYVGMGKQIAEEYKASDSIFEQASEALGFDV
                     KKMVFEGDDETLKITENTQPTILTASIACLQPLLQRGIRPDVAAGLSLGEYSAHVAAG
                     TMTFTDAVALVRKRGKFMQEAVPVGKGTMAAILGLESEAVIECCKRASTEGIVEPANF
                     NCPGQVVVAGEVKAVEKAVEIAKDMEAKRAMLLPVSAPFHCSMLKPAGEKLAVELEKI
                     NLNDMQFPVVANVNAEYILDKSKVKDLLIRQVSNSVLFEASIRKMLENGVGTFVEIGP
                     GKVLSGFVRKISRDVRVLNVEDIESLNNTLRELEN"
     gene            complement(6812..7807)
                     /locus_tag="PLA01_01675"
     CDS             complement(6812..7807)
                     /locus_tag="PLA01_01675"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPD00042.1"
                     /translation="MSTTYNCGILGLGSSVPEKVLTNYDLEKMVDTSDEWISKRTGIS
                     QRRIMDDNTPVYKLGVDAARKALDDAGITAEELDLIIVATETPDYLTPSMACIVQSKI
                     GARKAAAFDLNAACSGFIYGMTVANQFIKSGHYKYVLVIGCEGLSKIVDWEDRNTCVL
                     FGDGAGAAVFGPVEEDYGVITTYIGADGELGKNLTLPCCYIGPEDIEVRTGDNKKVLW
                     MDGSEVFKFAVRIMEHGAYRVLEDSGLSMEDIKLIIPHQANIRILEGAAKRLQVPKEK
                     VFTNLHKYGNISSASIPLGLDEAYREKLFSKGDNLILVGFGGGLTWGSAVIRWNK"
     gene            complement(7791..8810)
                     /gene="plsX"
                     /locus_tag="PLA01_01680"
     CDS             complement(7791..8810)
                     /gene="plsX"
                     /locus_tag="PLA01_01680"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPD00043.1"
                     /translation="MVILVDAMGGDNAPEAIVNGCLDAVGEAEGFDILLIGNEDKIRE
                     ILSKRTYDQSRIKIHHASEVITVEDTPTKAIKSKKDSSMVVGFKLLKERQGDIFLSCG
                     NSGALMAGALFIVGRVKGVDRPAIAAIVPTKKGNGLIMDAGLNTICKPINYLQFGVMG
                     SIYMKEMLGIDNPKVGLLNIGAEVGKGNETLKHAYALLSEANINFAGNVEGNDVPLGN
                     VDVVVCDGFAGNVMLKFYEGAASFLIGMLKDIYLKNIKTKLSALMIKKEMKQFKKVMD
                     ADVQGGAPILGVNGLVLKSHGSSNARTIKNVVIKASKFAKTKVIDKIREEFVNMEVEE
                     IEHDL"
     gene            complement(8866..>9221)
                     /locus_tag="PLA01_01685"
     CDS             complement(8866..>9221)
                     /locus_tag="PLA01_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HPD00044.1"
                     /translation="KSKDIVGELVDLTLGQGGISILETNESMAFEKTRIIQGHYIYSL
                     AESLAIAVIDAHVALVGVANIKYKTPVYAGSKLVAKAEVKKVKDNKYIVWVKISEKQV
                     ESFRGKFILVSLEKI"
BASE COUNT         2552 a         2351 c         1364 g         2954 t
ORIGIN      
        1 tcctccgacc caaaaaggcc atgctcaccg ctgtgaggat tgagacctgc aaccgccatc
       61 gtacctcctt ttataccgag aagttcaagg gctctcgtac aacgctgaat atagcttaaa
      121 accttttctt tattaacaag cccgcatgcc tgacttagcg aaacatgccg ggtcagaaag
      181 aacactcgga ggtctctaac ctcaaacatg gttaaaggat cccgggtgtt tgtgagctct
      241 gaaaatattt ctgtatgtcc aatacagtta atccctgcca ttttcaagga ctctttattt
      301 atgggtgtcg tcgccacagc atcaattacg cccttttgag ccagttcgac agagtgtttg
      361 atgtagctgt acgctgcctc tccacctatt gcttgaacct ttccggttat aaccccggat
      421 acatccacat cttcataatc aatcaggtta acaatacctt cattgtatat tccttcggat
      481 gcatccgtta ctttattaat tctgctgtca agtccgcaaa tctttaatgc tttattaaca
      541 gcatccctgc ttccgattac aaccggcctg ataaacctgt atatgctctt ctcccttaat
      601 gcctttacta ctatttccgg tccgattccc gcaggatccc cgagaggaat gcctattaca
      661 ggtttgtttg aaggcatatc cggaaaatta gccatgtcca attcaaaaac acctgccttt
      721 aaaaaattaa ctgtttaaca tcctgccgat aaaaaggtgt atatcaagta agttaacaaa
      781 atattcaaga gttacagtgt agaactgcac ttaaattaaa aaagctgttc taataagaac
      841 agccgcaaaa caacaattat gtaacaaagt tatctgggtt ctaccaagaa cttaattgca
      901 gtcctctcct ccccatcaat actaatagat gaaaaagctg gaacagtaat gatgtctatc
      961 ccattcggtg caacaaaccc tcttgtaatt gctatagctt taatagcttg attaactgct
     1021 gctgccccca ctgcctgtat ttctgctgtc gattttcccc taagaattgc tgctaatgct
     1081 ccagcgacca ttttcggctg cgattgagct gataccttta atacttccat acacttttcc
     1141 tcctcaataa aatataacta tttaaattaa ataaattaat ataaatcctg tctatctgtc
     1201 tatttattgt ttatgttaac caatattttg tagactctac ccacttacta ttttataaca
     1261 atgctcttca acaaacaata ccataaaatt aatattttat atcattaatt tcatgtacat
     1321 cgctacattc cctaaccatc acgctttcat aaccatactt ttccttcaaa taatttacat
     1381 tacttctttt ttgacctacg acatcagata ttctttttct acttgtacct attgttaaag
     1441 aacttttttt tgacaaatta ttctgttcta taatttcctc aattatactt aatgccagct
     1501 tggactccac caactgcctg atagcaggat gaaacggacc ggcaaccaca tcaccgtttc
     1561 cattgatact ctctgtaggc tgaagcccaa tcctaataac atttatattg ttctcccggt
     1621 atatacgaag aagctttgca caaagtgcga cagcgtcttc caaattgaga ggagtatact
     1681 cacctttatt atacatcttc tctaaatatg tatcctttat aaccagtgta ggatatatac
     1741 ggagcaaatc cggctttagc cccacaacct ttttcgccgt ttccaaagcc ttttcatcgg
     1801 tatcccccgg aagcccgatc attgtctgaa tacctaaaac aaaaccatat tctttaatga
     1861 gttttgaaga atcgaatact acctcttgac tgtgccccct gcagcttttt tcaaggactt
     1921 cattatcaag actctgtaca cccaattcta ttgtcctgac tttatattct ttcaaatagt
     1981 caagaatatc cccgtttata taatcaggcc gtgtggatag cctaattgcc tttatcttgt
     2041 cttttttaat gtattcgtta gctatcttga ggaattttaa ctgttcactc ttttcaatac
     2101 ccgtaaagct tcctccataa aacgccatct caacgtatgc atcattaccg gctgtactca
     2161 gacaggactc aatctcagat accatctttt cttcggtcat ttcatcaatc tgaccgctta
     2221 ttttcttttg attgcagtat atgcagtcaa agggacagcc tttatgggga ataaatatag
     2281 gtattataat gtgtttggtt tccattcttt tcccttccgt atcaattacg ataaacatat
     2341 attttgtcat tttcttcacg aaatactttt attacacggc aaaattaaat cattattttc
     2401 tccagggaag actttgctgc catctgctct gcttcctttt tgctccttcc cataccgata
     2461 cccaccgttt tttcatctat tttcacttga gagacaaaca tcttgctgtg atccggaccc
     2521 ttttcctcaa ttatctcata ggaaattgta tggtcactgg ttttctgaac atactcctgc
     2581 agctgtgttt tgtaatccgt atatatagta cctttgattg ctccattaat tgaatcttcc
     2641 agttgccgca aaataaaagc tttcgcactt tcgaatccgc catccaaata aatagctcct
     2701 attagagcct caacagcatc ggaaagtata gaggttcttt tccttcctcc cgaattttcc
     2761 tctccttttc ccagtaaaag atagttccct atgtttattc tttttgcaca ctttgccaac
     2821 gaccgctcac atactacatt agccctgaat tttgtcatct ctccttcggt aaaatcggta
     2881 gcattaaggt aaatatgctc gctaactacc atttccaaaa ccgcatcacc caaaaattcg
     2941 agtctttcat tgctgaccat cttttcattc ctgcattcat tggcaaaaga actgtgggtt
     3001 aaagccagca cgagtttctt tttgtcttta aagcaataat cgataatttt ctcgaactca
     3061 tctgttttaa ccgacagagc ctttaaatcc ggcattgctg ctcccccatt cttcagtact
     3121 agtatgtctt gtacattata ttatatctca taagtttata atgcatctct tcaaaagaaa
     3181 atttacgccc gactctttat aacacgacag ttcaaataag tattttattt atcaaagtat
     3241 aaagggaaca gtcagtaacc gtccccccta ataagttgtg tataaacata ttatatcaga
     3301 attttttcaa aacaacagtt gcattatgcc cgccaaagcc taatgaattg gacgcagcat
     3361 aagttatatc agcctttctt ccaacattcg gtacatagtc caggtcacat tcaggatcgg
     3421 gatttctgta attgattgtt gggggaaggt aaccttcttt aagtgtcaga gctgttataa
     3481 tacactccac tgctcctgcg gcaccgagaa gatgccccgt cattgacttt gtcgaactca
     3541 ccggcagctt gcctgcatgc tcaccaaata cagtcttaat agctgcagtt tcgaacttgt
     3601 cgttgtactc tgttgacgta ccgtgtgcat taatataccc gacttcttca ggcttaatac
     3661 ccgcatcatt tatggccatt tgcatacact ttgcaccgcc ttcgccttcg ggagcaggag
     3721 cggtaatatg atatgcatca tttgttgaac cgtatcccac aatctctgct attatgtcag
     3781 ctcctctttc tttagcatgc tccaaatctt caagtattaa tatacctgca ccttcgccca
     3841 ttacaaagcc gtttctctca gcatcaaacg gcctgcacgc caaattgggg tcttccgttg
     3901 tagacgtggc cttcattgaa caaaatcctg caaaagccat gggagtaatt gaagcctccg
     3961 ccccaccggt aataattaca tccgcatcgc cacgctgaat aaccttgaaa gcatccccta
     4021 tggcatttgt tgatgtggca caagccgtaa ccacacattc attaaatccc tttgcaccaa
     4081 actcaatagc tattcgtcca gtagccatat ttgaaatcat catgggaata aaaaacggac
     4141 tgactcttcc gggtccctta tcaatatata ctctgaactg ctcttcaaat gttgttattc
     4201 ctcctatacc ggaacctaca ataacaccga acctgtacgg atcaattttt tcactatcga
     4261 ttcccgacat gttatatgcc attttagccg cacacaatgc atattgggta tagatatcca
     4321 ttcttttggc ttctttttta tcaataaacc tggtaggatc aaagtcctta atttcagcag
     4381 caacctttgt aggcatacat gacacatcaa attttgatac tgtggttatc ccgcattttc
     4441 cttcctttat agagttccaa aactcattaa tatcagtacc taatgcagac acaacaccca
     4501 taccggttat aactacacgc cttttcattg tttaacctcc ctgcctgtta catcataagt
     4561 atatatccaa agattaatta ttgtatgtac caataattaa catcaaaacc aactctgtgc
     4621 aataccccaa ttcataaaaa agtccctttt tcagggactt ttttatgaat tattcttaat
     4681 atactcaacc acatcgccta ctactgaaat tttctcggcc tcgctatcag gaatttcgat
     4741 gtcgaactcc tcttccaaag ccattatcaa ttcaacaata tccagtgagt cagcacccag
     4801 atcgtctatg aaagatgatt ccattgtaat ctcatcttca tcaacgccta actgctctgc
     4861 tataatcttc tttacctttt cgaacactat cattcacctc cttccacagg gggttttgca
     4921 tgattagaac ccttcgtgta aaactgtatc gacttaacca aatagtgtta caatatgata
     4981 ttattacatt actaacccgc cgtcaatatg tattacctga cctgttacat aatctgagtc
     5041 atcagatgcc aaaaaactaa ccacatccgc cacgtcttca ggcgtcccaa accttttcat
     5101 gggaatgtta ttcaaatagt tttccttaac attgtcaggt aaaacatcgg tcatcttact
     5161 cattatcaca cccggtgcaa cagcattaca gtttatacct cttgatgcca gttccttggc
     5221 tgtcgacttt gtaagaccaa tcatgccggc tttggctgct gagtaattgg cttgcccgac
     5281 attgcccata acccctgcaa ccgaagcaat attaataatt ttaccgcttt tttgtttcag
     5341 cataactttc gaagccgctt ttgtacaaag gaacgcaccc tttaaattga tatttaagac
     5401 atcatcccat tcctgttcag tcattctcag tataaggtta tcccttgtta taccggcatt
     5461 gttaacaagt atgtcaacgc ttccaaaagc ttcaacagcg gttttaacca ttgcctcagc
     5521 atcctcaaaa tttcttacat caccctttgt aacagcaaca tttattccgg ctgctttaaa
     5581 ttcctccgca gtttggtcaa tgctgtccga agcaggtata tcgtttagaa caatattggc
     5641 acccatgctt cccaacttaa acgcaattgc tttgccaaga cctctgcctg aaccggttat
     5701 aattgcagtt ttccccttta gtttcatact attcacgtct ccttgccatt agtctatttg
     5761 aattcagaaa gtgaacccca ccttcctgta atctttcaac agcatatttt ttatattcta
     5821 attttcaagc tcccgaagtg tattgttcag agattcaatg tcttcaacgt taaggactct
     5881 gacatcccgg cttattttgc gtacaaagcc gctcaatacc tttccgggtc ctatttcaac
     5941 aaaggtaccg acgccgtttt ccagcatttt tcttattgaa gcctcaaaca gtaccgaatt
     6001 acttacctgt ctgattagaa gatccttaac ttttgattta tctaaaatat attctgcatt
     6061 cacatttgct actacaggaa attgcatgtc attcaaattt attttttcca actcaacagc
     6121 aagtttctca cctgcaggct ttaacatgct gcaatggaaa ggtgcactga caggaagcag
     6181 catagcccgc tttgcctcca tatcctttgc aatctccaca gccttttcca cagcttttac
     6241 ctctcctgcc acaacaacct gccccggaca gttaaagttt gccggttcca caattccttc
     6301 cgttgatgct cttttacagc actcaataac agcctcggat tcaagtccaa gtatggcagc
     6361 catagtacct ttacccacag gaaccgcttc ttgcatgaat tttccccttt tgcgaaccag
     6421 tgccactgca tcggtaaacg tcatggtccc tgccgcaaca tgggcagaat actcaccaag
     6481 actcaatccg gcagctacat caggccttat acccctttgt aaaagaggct gaagacaagc
     6541 aatacttgca gtcagaatcg ttggttgtgt attttcggtt attttcagtg tttcatcgtc
     6601 accttcaaaa accatctttt tcacatcaaa acccaaagct tcggatgcct gctcaaatat
     6661 agaatctgaa gctttatatt cctcagctat ctgctttccc atccctacat attgggcacc
     6721 ctgcccggaa aataagaatg ccagttttcc catcatgttc cctccaatat tgcaataaat
     6781 aaggtattac ctttttacgt tattcttcat gctatttatt ccatcggata acagcagatc
     6841 cccatgtcag tccgccgcca aaacctacca gaattaaatt gtctcccttg gaaaatagtt
     6901 tttctctata ggcttcatcc aaccccaagg gtatggatgc cgaagaaata ttaccatact
     6961 tgtgaaggtt cgtaaatacc ttttctttcg gtacttgaag tctctttgcc gctccctcca
     7021 aaatacggat attggcctga tgaggtataa tcagctttat atcctccatg gaaagaccag
     7081 aatcctccaa aaccctgtac gctccatgtt ccattattct tacagcgaac ttgaagacct
     7141 cactgccatc catccaaaga accttcttat tatctcctgt ccttacttca atatcctcag
     7201 gtcctatata gcaacacggc agggtgagat ttttacccaa ctcaccgtca gcaccgatat
     7261 atgtggttat aacaccataa tcttcttcca ccggaccaaa cacagccgct cccgctccat
     7321 cgccaaagag tacacaagtg tttctgtcct cccaatccac tatttttgag agcccttcac
     7381 atccaataac cagtacatat ttgtaatgtc ctgatttaat aaactggttt gccaccgtca
     7441 ttccataaat gaatcccgaa caagccgcat taagatcaaa tgccgctgcc ttccgtgctc
     7501 cgatcttgct ttgaacaata caggccattg acggagtcag atagtcaggg gtctcggtag
     7561 ctacgattat caggtccagt tcctctgctg ttattcccgc atcgtccagg gctttccttg
     7621 ctgcatccac tcccaatttg tatactgggg tattatcatc cataattctc ctttgagata
     7681 ttccggttct ttttgaaatc cattcatccg atgtatctac cattttttcc agatcatagt
     7741 ttgtaagtac cttttcaggc acactgctgc ctaatcccag aattccacaa ttataagtcg
     7801 tgctcaatct cttcaacctc catattaaca aactcttccc taattttgtc gataacctta
     7861 gtttttgcaa atttgctggc cttaatcact acattcttaa ttgttctggc gtttgagctg
     7921 ccatgacttt tcaaaacaag accgttaacc ccaaggatcg gagcaccccc ctgaacatca
     7981 gcatccataa cttttttaaa ttgcttcatt tccttcttaa tcatcaaagc cgaaagcttt
     8041 gtttttatat tttttaagta aatgtccttt agcattccga taagaaacga agcagcaccc
     8101 tcatagaact ttaacattac attccctgca aaaccgtcac atacaaccac atcaacattg
     8161 cccaaaggca catcatttcc ctcaacattt ccggcaaagt ttatattggc ttcagacagc
     8221 aaagcatatg catgctttag cgtctcatta ccctttccta cctcagcccc tatgttaagc
     8281 aagccaacct ttgggttatc aatccccagc atttctttca tgtatatgga acccataaca
     8341 ccaaactgta aataattaat tggcttgcag atggtattca gtcctgcatc cattatcagc
     8401 ccattccctt tctttgtggg aacaatagcc gcaattgcag gcctgtcaac ccctttcact
     8461 ctgccaacta tgaataatgc acccgccatc aaagccccgc tgttgccgca tgacaggaaa
     8521 atatcaccct gtctttcctt caacagctta aaaccgacaa ccatggaaga atccttcttg
     8581 ctcttaattg cctttgttgg agtatcctct actgttataa cctcagaggc atgatgaatc
     8641 tttatgcgag attgatcata agtccttttg ctcaatattt ccctgatttt gtcctcatta
     8701 ccgattagta atatatcaaa accctcagcc tcaccaacag catccaaaca tccgtttaca
     8761 atagcctccg gtgcattatc tccacccatt gcatcaacca atataaccaa agtaacttca
     8821 ctcccaatat caatagtaat ctatcaatat aaattactat gaatactata ttttttcaag
     8881 ggataccaaa ataaatttgc ctctgaaact ctcgacctgt ttctccgaaa ttttcaccca
     8941 tactatatat ttattatcct ttaccttttt aacctctgcc tttgctacaa gcttgcttcc
     9001 tgcatataca ggcgtcttat acttgatatt tgccacccct accaatgcca cgtgtgcatc
     9061 tataacagcg attgcaaggg attcggcaag ggaataaata taatggccct ggataatcct
     9121 tgttttttca aaagccatac tctcattggt ctcaagtatt gaaattccgc cctgtcccag
     9181 agtaagatca acaagttctc ctacaatatc tttgctcttt a
//
LOCUS       DAOOJC010000032         9172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_6628_length_9172_cov_4.945048, whole genome shotgun sequence.
ACCESSION   DAOOJC010000032 DAOOJC010000000
VERSION     DAOOJC010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 9172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9172
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6628_length_9172_cov_4.945048"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLA01_01690"
     CDS             <1..240
                     /locus_tag="PLA01_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPD00045.1"
                     /translation="VKTYDAYEDVEKYAAVVDLKTIEENDYNLNISRYVDTTEEEEEV
                     DIELVLQEIRELKTNIADTEEKLNVYLQELGFEVI"
     gene            227..1510
                     /locus_tag="PLA01_01695"
     CDS             227..1510
                     /locus_tag="PLA01_01695"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HPD00046.1"
                     /translation="MKLFKGVEDIMEEVYSKTELEKIPNGWQVRKFSDVIEKIVGGGT
                     PSRKIPQYFYGDIPWATVKDLDNSFYKYDTIEHISLDAVENSATNVINSNKIIIATRM
                     GLGRGFINKVPMAINQDLKGIYLKDNMSTEFLMFWYLYNRSNIEMMGKGSTVKGIRQE
                     ELKEMPILVPPLKEQQKIADILSIVDEQIEQTDALIEKTKELKKGLMQRLLTKGIGHT
                     DFKKTEVGEIPVEWEVKKLGEVTEIISGGTPNTKVEEYWRNGDICWATPTDITSSGKY
                     IRKTEKHITEKGLKNSSANLLPIGSILMTSRATIGERCINTVPMATNQGFKSFICREQ
                     VILNEFLYYLIEIIKPIFIRLAGGSTFLEISKKQVEEQKIQVPPIEEQKKIAFILSVI
                     DIKLEDYQTKKQKLQTLKRGLMQKLLTGKIRVKVS"
     gene            1522..4671
                     /locus_tag="PLA01_01700"
     CDS             1522..4671
                     /locus_tag="PLA01_01700"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HPD00047.1"
                     /translation="MSYLGNEETLVELPAIQYLQDKLGYRFIHGDLLTPEKGERDSYT
                     EVILVNRLEKALKRINPWMNIDSINKTIRYLTRPENLGASLLEINEKIYDAMVNLNYA
                     LEQAVGGSRQKKFHTVKFIDWANIDNNEFIVTRQFKVQGPSEKIIPDIVIFINGIPVV
                     VIECKSPFLEKSKNENMGKYEAFTQLRRYMNLRNSDILEGAPRLFYTNFFTCILNKYH
                     GYIGTISSGYGYYLEWKDPYPFTKNQIDDVENSGQNVLLQGVLEKNNLMDIMRNFILF
                     EADEDSGTRIKKICRYQQFRAVNKALQRLVNGKDALSRGGVIWHTQGSGKSLTMVFLA
                     RKIRRTPELIDATIVIVTDRIDLDKQIYNTFVRTLSSITTPVRADSVAQMKELLSRAQ
                     PQIIMTTIQKFQSEKDDKEILKDNNQESVLVFEKEFPVLTVKSNVIVLADEAHRSQYK
                     GMAKNMRDALPNAAFLGFTGTPIDREDKSTPRTFGSYIDKYTIQQAVDDGATVKIVYE
                     GRKPELQIKADTLEELFNQAFEDKTEEEKEAIKQKYANKTTVVEADDRIEDIAKDMLL
                     HYKEQVYPNGFKAQVVCVSRGACVKYYNALNKYMEEIMGESLEVKIIFSGNLNDPSHL
                     KERFTTKAEQDAIISRFKKPIDKDKLCFLIVKDMLLTGFDAPIEQVMYLDRPLKEHNL
                     LQAIARVNRTCGEKKKCGYVVDYFGISNFLEEALLIFDKEELGQPMQSMDGIYKQMLS
                     YRESVMGMFTGIDKNNLDALVKVLEPEDKRAEFELAYKRFASTMEQILPNHVPMENIN
                     DLRWLSYIRAAAKARFEPQKDLDISDCGEKVKSIINDHLKSLGVIQWISPITLFDSDF
                     HNKVRTLKSDESMASAMEHAVEHIIYVKMDDNPVYYTSLLEKLQKILDETKNNWIERK
                     KKLEEFINRDVKTGEIEEADALGLTKKEYAFFETVRKHLSDSDDLKSKGTKENVALFI
                     TEEVIELSKDIAKNVADIVQKNYMIDWVNNQAKSSEIERQIFMLLNTKYFKKIKMTDR
                     KKLVQPLLQLAKKHFANLE"
     gene            4682..5395
                     /locus_tag="PLA01_01705"
     CDS             4682..5395
                     /locus_tag="PLA01_01705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HPD00048.1"
                     /translation="MRLSIRYGTKIIEFDVEYKERKTMEISVELPDKVSVVAPLGTTQ
                     EVIEYKVKSKAAWIVQKLYTFRDMEYRRIPREYVNGESFMYLGRNYSLQIQVDENVKK
                     PEVRLYQGKFHVIAPSKNEDMIKQAMEEWYREKTREKVLERVQYYQHFFKKRPYDVKA
                     KEQKKRWASCTSKDELLFNWRCVMAPSYVLDYIVVHEMCHMYYKNHSQEFWDMLAAIM
                     SDYERRKEWLKKYGVRMDL"
     gene            5878..6918
                     /locus_tag="PLA01_01710"
     CDS             5878..6918
                     /locus_tag="PLA01_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00049.1"
                     /translation="MEYKVTISDGTVLEKSIQHVSYIVYPPDDYDIRRNYPRNSMVIS
                     GIIDIGEGTVGLYKWALIPGTNKECYKDVTVEHFYAGQLVSKVKFSKAFVVDYSESYS
                     NSEGVGYFTIYLRQFAGMDIEHSNEISQEQQKKYQEQVQTNIEQTSELEKAEPKISSA
                     VSLDNVNNVNSKPVMRITDRLKNQKDPDTLLTKEEVKLRYAKEIEASKEAYKRAIDMA
                     KKKKVKGCVASNGKITLSGFDGKTTKAPEGFARVPLDTMFDYCKKIGHELPPKVPAFY
                     DNGREGSYFACHAEKQLSLLTDEPIGISRDMCPDCVEYFKKHAAYTNQVKITADPKKT
                     RIFFPDGRVEEF"
     gene            6934..7554
                     /locus_tag="PLA01_01715"
     CDS             6934..7554
                     /locus_tag="PLA01_01715"
                     /inference="COORDINATES: protein motif:HMM:NF025779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm5 family immunity protein"
                     /protein_id="HPD00050.1"
                     /translation="MTNELVQLLNKAKDELNKNPKGQLVLPLRKEIYRLMGERIEDNE
                     GHAIKTEGYFRRLKLAVLCVNHVLSIWENVMPNDNTPANLLKSINDYLSGKKDWDCLW
                     EEQNDFWAVLDNYLCDGNDYGNSIYVGHASINAVMVALNDEDLGGEDYINIFDEDLDP
                     YTWDTAFYASLAYSENESYDEETKIQKRREFWLWYLNEGVMKAYNI"
     gene            7804..8034
                     /locus_tag="PLA01_01720"
     CDS             7804..8034
                     /locus_tag="PLA01_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00051.1"
                     /translation="MTNLYEFKSDERFKELSSNVISLVEKLDNVLHNKGYIYKGVWRN
                     DEEPGGKVFIFEVERRKGRRKNLITLRPTNPI"
     gene            8437..8907
                     /gene="tnpA"
                     /locus_tag="PLA01_01725"
     CDS             8437..8907
                     /gene="tnpA"
                     /locus_tag="PLA01_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HPD00052.1"
                     /translation="MDKNTLSHTTWECKYHLVFVAKYRRQVIYGKIKQDIGRILRELC
                     ERKGIEIIEAELCKDHIHMLVRIPPKYSVSEIMGYLKGKSSLMIFDRHANLKYKYGNR
                     HFWCRGYYVDTVGKNAKKIEEYIRKQLQEDIANDQISLKEYMDPFTGEQVKKRK"
     gene            complement(9038..9114)
                     /locus_tag="PLA01_01730"
     tRNA            complement(9038..9114)
                     /locus_tag="PLA01_01730"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9078..9080),aa:Ile,seq:cat)
BASE COUNT         3350 a         1260 c         2080 g         2482 t
ORIGIN      
        1 gtgaagacat atgatgctta tgaagacgtg gagaagtatg cggcagtggt tgatttaaag
       61 accattgagg agaatgatta taacctgaat atcagcaggt atgtggatac gacggaagaa
      121 gaggaagaag tggatattga gttggtattg caggaaataa gggaattgaa gacgaatata
      181 gcagatacag aagagaagtt gaatgtgtat ttacaagagc tggggtttga agttatttaa
      241 gggagtagaa gatattatgg aggaagtata tagcaaaaca gaattagaaa agatacccaa
      301 tggatggcaa gttagaaaat tcagtgatgt tattgagaaa atcgttggtg gagggactcc
      361 atcccgaaaa attccacaat atttttatgg ggatatacct tgggcaactg tgaaagattt
      421 agataacagt ttctataaat atgacactat tgaacatatc agtttagatg cggttgaaaa
      481 tagtgcaaca aatgttatta atagtaacaa aattattatt gctacaagga tgggtttggg
      541 tagaggattt attaataagg ttccaatggc gattaaccaa gatttaaaag gaatttatct
      601 aaaagataat atgagtacgg agtttttgat gttttggtat ttatataata gaagcaatat
      661 tgaaatgatg ggaaaaggtt caacagtaaa aggtattaga caagaagaat taaaagaaat
      721 gccaattcta gtgccgccac ttaaagagca gcaaaaaata gcagacatcc tatctattgt
      781 agatgaacaa atagaacaaa ctgatgcttt aattgaaaag acaaaagaat tgaaaaaagg
      841 attaatgcag aggttattaa caaaagggat tggacatacg gattttaaaa agacggaagt
      901 tggggagata cctgttgagt gggaagttaa gaaattaggg gaagttactg aaataattag
      961 cggagggaca ccaaatacta aggttgagga gtattggcgt aatggagata tttgttgggc
     1021 tacaccgacc gatataactt ctagtggtaa atatatacgc aaaacagaaa agcatataac
     1081 cgaaaaaggt ctaaagaata gttctgccaa tttgctacct attggtagta tcttaatgac
     1141 tagtagggca actatagggg agagatgcat aaatactgtt cctatggcta caaaccaagg
     1201 atttaagtct tttatatgta gagagcaagt aatattaaat gagtttttat attacttaat
     1261 tgaaattatt aaacctatat ttattagact ggcaggagga agcaccttct tagaaatatc
     1321 taagaaacaa gttgaagagc aaaaaataca agttcctcca atagaagagc agaaaaaaat
     1381 agcattcatc ttatcagtaa ttgatattaa attggaagat tatcaaacca aaaaacaaaa
     1441 actacaaacc ttaaaacgag gccttatgca gaagctactt acaggtaaaa tcagagtaaa
     1501 agtatcatag ggggcgacag catgtcttat cttgggaatg aggaaacact tgtagaattg
     1561 ccggcaatcc aatatttaca ggacaagctg ggatatcggt ttatccatgg agacctgttg
     1621 actcccgaaa agggagaaag ggattcatac acggaagtaa tccttgtcaa caggcttgaa
     1681 aaggcactta aaagaatcaa tccatggatg aatatagaca gtatcaataa gactattcgc
     1741 tatttgacta ggcctgagaa cttgggagca tcattgcttg aaataaatga aaagatttac
     1801 gatgcaatgg ttaacttaaa ctatgcgttg gaacaggctg tgggcggaag caggcagaag
     1861 aaattccata cagtaaagtt tatcgattgg gctaatatcg ataacaatga atttatagtt
     1921 acaaggcaat tcaaagtaca aggacccagt gaaaaaatca ttcccgatat tgtgatattt
     1981 ataaacggta taccggttgt tgtcattgag tgcaagtccc cgtttttgga aaaaagcaag
     2041 aatgagaaca tgggtaagta tgaggcattt acccaactga gaagatatat gaatttgaga
     2101 aactcagata ttctagaagg tgctcccagg cttttctata caaacttttt tacttgtatt
     2161 ctaaataaat accatggata tataggcact atcagttcgg gatacgggta ctatcttgag
     2221 tggaaagacc cttatccttt taccaaaaat cagattgatg atgttgaaaa cagcggacag
     2281 aatgttttgc ttcaaggagt attggaaaag aacaatctga tggacattat gcgaaatttt
     2341 attttatttg aagctgatga ggattcggga acaaggatta aaaagatctg tagataccag
     2401 cagtttagag cggtaaacaa ggcattgcaa aggttggtca acggtaagga tgccctgtct
     2461 cgcggagggg ttatatggca cacccagggg tcagggaaat ctctaacaat ggtatttctt
     2521 gcccggaaaa tcagaagaac tcccgaatta attgatgcca ccattgtgat tgttaccgat
     2581 agaatagatt tggataagca aatatacaac acatttgtaa ggactctttc gagcattacc
     2641 acaccggtaa gagctgatag tgtggcacaa atgaaagaat tgctgtctag ggcacagccg
     2701 caaattatta tgacaaccat tcaaaagttc cagagtgaaa aagatgataa ggaaatttta
     2761 aaggacaaca atcaggaatc ggtgttggtg tttgaaaaag aatttccggt gttgactgta
     2821 aaatccaatg taattgttct cgctgacgaa gcacacagga gtcaatataa aggtatggct
     2881 aaaaacatga gggatgccct ccccaatgcg gcattccttg gctttaccgg aacacctatt
     2941 gacagggagg ataaatccac accaaggacc tttggctcat acattgataa atacaccatt
     3001 cagcaggcgg tagatgacgg agcaacggta aagattgtat acgaaggaag aaagccggag
     3061 cttcaaataa aagcggatac ccttgaggaa ctttttaatc aagcctttga agacaaaacg
     3121 gaagaagaaa aggaagctat aaagcaaaag tatgcaaaca aaactacagt tgttgaagcg
     3181 gatgacagga ttgaagatat tgcaaaagat atgcttttgc attacaaaga acaggtatat
     3241 cccaatgggt tcaaggctca ggttgtatgt gtgtccagag gggcatgtgt taagtattat
     3301 aatgctctga acaaatacat ggaagaaatc atgggtgagt cactggaagt aaaaattata
     3361 ttttcgggaa acctcaatga tccgtcgcat ttgaaagagc gttttacaac taaagcagaa
     3421 caggatgcca ttataagcag atttaaaaag cccatagata aggacaagct atgtttcctt
     3481 atagttaagg atatgctttt gacgggcttt gatgctccaa tagagcaggt aatgtacctt
     3541 gacaggccgt taaaagagca caacctttta caggcaattg cgagggttaa cagaacatgt
     3601 ggagaaaaaa agaaatgtgg atatgttgtg gattatttcg gtatttcaaa cttccttgag
     3661 gaggctttgc tgatatttga caaggaagaa ctgggacagc cgatgcaatc catggacggt
     3721 atttataagc agatgctgtc ttatcgggaa tccgttatgg gaatgtttac agggatagat
     3781 aaaaataatc tggatgcact ggtaaaggtg ctggagcctg aagataaaag ggcggaattt
     3841 gagcttgcat ataaaaggtt tgcgagtaca atggaacaaa tacttcctaa ccatgttccc
     3901 atggaaaaca tcaatgattt gagatggctc tcgtacatcc gtgccgctgc aaaggcaaga
     3961 tttgagcctc aaaaggattt ggacatatct gattgcggag aaaaggtaaa gagtattata
     4021 aacgaccatt taaaatcttt gggtgtaata cagtggataa gcccaataac attgtttgat
     4081 tcggattttc acaataaagt tagaacattg aagtccgatg aatccatggc atcggctatg
     4141 gaacatgcag tggaacatat aatttacgtt aaaatggacg acaaccctgt atactataca
     4201 agtttgttgg aaaagttaca aaagatattg gatgaaacca aaaacaactg gatagagaga
     4261 aaaaagaaat tagaagaatt tatcaatagg gatgttaaaa ccggtgaaat tgaggaagca
     4321 gatgcattgg gacttacgaa gaaagagtat gctttctttg agaccgttag aaagcatttg
     4381 tctgattccg atgatttaaa atcaaaggga acgaaagaaa atgtggcatt attcataact
     4441 gaagaagtga ttgaactgtc aaaagatatt gcaaaaaacg ttgcagatat tgtgcaaaag
     4501 aactatatga tagactgggt taataaccag gcaaagtcgt ctgagataga gcggcagata
     4561 tttatgcttt taaatacgaa atattttaag aagataaaaa tgacagacag aaaaaagctt
     4621 gtacagccgt tattacagct tgcaaaaaaa cattttgcca atttggaata gagggaaata
     4681 tatgcggtta tccatacggt atgggacaaa gataatagaa tttgatgtgg aatataaaga
     4741 aagaaagaca atggaaatta gtgttgagct gccggataag gtatctgtag tggcaccttt
     4801 gggaacaacc caagaggtta tagaatacaa agtcaaaagc aaagcggcgt ggattgttca
     4861 aaaactgtat acctttaggg atatggaata tagaagaatt ccccgggaat atgtaaatgg
     4921 ggaatccttt atgtatttag ggcggaatta ttctctgcaa attcaagtag atgagaatgt
     4981 taaaaagccg gaagtgaggt tatatcaagg aaagttccat gttattgcac caagtaaaaa
     5041 tgaagatatg attaagcagg caatggaaga gtggtacaga gaaaagacac gggaaaaggt
     5101 gttggagagg gtacagtact atcagcattt ttttaagaag aggccttatg atgtaaaagc
     5161 gaaagaacag aaaaaacgct gggcaagctg cacttccaag gacgaattgt tgttcaattg
     5221 gaggtgcgtt atggctccct cctatgtgct ggactatatt gttgtgcatg aaatgtgcca
     5281 tatgtattat aagaaccatt cccaggaatt ttgggatatg ttggctgcaa taatgtcgga
     5341 ctatgagagg cgtaaggagt ggctgaaaaa atacggtgtc agaatggatt tgtgatttat
     5401 tgataaggat gaggtggagg gctattggat gggaaaagta attacaaata attctatatt
     5461 ttttataaat ttatgaagga gaggaaaaga ctatatagaa tatatactta taaattgtaa
     5521 acttattaga atttagaagc actttaatgt caattgctgc tactgcttcg gataaatgat
     5581 ccaaagttgc tttagttcca tattcgtttg caaaaaagag gtaagaaaga gtaatggtta
     5641 tgagtgtagg atcttaagtt ttttaaaaag ataaaaaagt cttgacgcaa tatgcctaaa
     5701 cataagtact gggatgtcct catatttgtt gttaatagac atgctgttga tattatggaa
     5761 aaattataaa ttgaatttga tagattatta tgtttttgct aataaattct aaagagttct
     5821 tctttataaa tccatggaga agaatcaagt atgtaaatag atggggggag ggataatttg
     5881 gaatacaagg taacaatatc agatggtact gtattagaaa aaagtataca acatgtttct
     5941 tatattgtgt atccacctga tgattatgat attagaagaa actatccaag aaactcaatg
     6001 gtaatttcag ggataattga cattggtgag ggaactgtgg ggctttacaa gtgggcttta
     6061 attccgggaa ctaacaagga gtgctataaa gatgttacag tagaacattt ttatgctggt
     6121 cagttggtga gcaaggtaaa gtttagtaaa gcatttgtag tagactactc cgaaagctat
     6181 tccaatagcg aaggtgtagg atattttacg atttatctaa gacaatttgc agggatggat
     6241 attgaacata gtaatgaaat atctcaagaa caacagaaaa aatatcagga acaggtccaa
     6301 acaaatatag agcaaacgtc agaactcgaa aaggctgaac ctaaaataag tagtgccgtt
     6361 agccttgaca atgttaataa tgttaattca aagcctgtta tgcgtattac agacagatta
     6421 aaaaatcaaa aagaccctga tacattgtta actaaagaag aagtaaaact aagatatgct
     6481 aaagaaatag aggcatctaa agaagcatat aaaagagcaa tagatatggc taagaagaag
     6541 aaagtaaagg gttgtgttgc ttcaaacgga aaaataactt tgagtgggtt tgatggaaaa
     6601 acaacgaaag cccctgaagg gtttgcacga gtaccattgg atacaatgtt tgattactgt
     6661 aaaaagatag gacatgaatt gcctccaaaa gtacctgcct tttatgataa tgggagagaa
     6721 ggaagttatt ttgcttgcca tgcagaaaaa caattgtctc ttttaaccga tgaacctata
     6781 ggtatttcaa gggatatgtg tcctgactgt gttgaatatt tcaagaaaca tgcagcatat
     6841 acaaatcagg ttaagataac agctgaccct aaaaagacaa gaatattttt tcctgatgga
     6901 agagtagagg aattttagga aggatgaaat attatgacaa atgaactggt tcaattactt
     6961 aataaagcaa aagatgagtt gaataaaaac cctaaaggtc aattggttct acctttaaga
     7021 aaagaaatat acaggttaat gggtgagcgt attgaggata atgaagggca cgctataaaa
     7081 actgaaggtt atttccgcag actgaaactt gctgttttgt gtgttaatca tgttttgtcg
     7141 atatgggaaa atgtgatgcc taatgataat actcctgcta atcttttaaa gagcataaat
     7201 gattacttgt cagggaagaa ggattgggat tgcttatggg aggaacagaa tgatttttgg
     7261 gcagtattgg acaactactt gtgcgatgga aatgattatg gtaattcgat ttatgtagga
     7321 catgcctcaa tcaatgctgt aatggttgca ctcaatgatg aggatttggg aggagaagat
     7381 tatattaata tatttgatga agatttggac ccatatacat gggatactgc tttttatgct
     7441 tctcttgcct attccgaaaa tgaatcgtat gatgaagaaa ctaagataca aaaaaggcgg
     7501 gagttttggt tatggtatct taatgaaggt gttatgaaag catataacat ctaatcgctg
     7561 agaatatagt aaaatttttt cagataactt gttattaatt atttattatt tgtattaatt
     7621 tgacgataaa gctagtagaa aaattaacag tcgtaattaa aattggaaga actgtagcag
     7681 gaaacagctt taagcttgaa tgttccgcaa ataggtaatg cggtagacta agctgtcgga
     7741 agcattttag tgcattctac gaaatactat gaacctgtaa aaaaacaaaa cgaggtgaat
     7801 tatatgacga atttatatga gttcaaatcg gatgaaaggt ttaaagaact ttcttctaat
     7861 gtaataagct tagttgaaaa gttggacaat gtattgcata acaaaggata tatatataaa
     7921 ggcgtatgga gaaatgatga agagccggga ggaaaggtct tcatatttga agttgaacga
     7981 agaaaaggga gaagaaaaaa tttaataacg ttaaggccca caaatcctat ttaaaagttg
     8041 aagtatattg gggtcagtat gataagaact attttgatgt aaacagtgta gataatatac
     8101 cggacaaatt acttgatgaa attgatgaaa tgtataacat gattgcttat taagtaagag
     8161 gaaatattcg ggaaaagatg catagggcaa tataatataa ccggtggcgg gaagtcattt
     8221 tcagaatgtg cgttttgtaa taatgctaac cgccatggtt taccgttaaa cagggtagca
     8281 acagtgctac cctgttttgc gttggtgtca gccaataaac ccctagctat gctaggggag
     8341 ccaatgcgct ttagcttcaa gtagaaaaat aaaagcctcc aatgttaaaa tagtgtgggt
     8401 ttgccaacca cattacaaaa cagaggaggg tcccaaatgg acaaaaatac tttatcacat
     8461 accacatggg aatgcaaata tcacttggtt tttgtagcga aatatcgtag acaagtgatc
     8521 tatggaaaga ttaaacaaga tattggaaga atacttagag aattatgtga aagaaaaggt
     8581 atagaaataa tagaagcgga gttatgtaag gatcacatac acatgcttgt gagaatacca
     8641 ccgaaataca gcgtatctga aataatggga tacttgaaag gtaaaagttc actaatgata
     8701 tttgacaggc atgcaaactt aaaatacaaa tacggaaaca gacatttttg gtgcagaggt
     8761 tattacgtcg atacagtagg aaaaaatgca aagaaaattg aggagtatat cagaaagcag
     8821 ctacaagaag atatagcaaa tgaccaaata agtctaaaag agtatatgga cccgtttacg
     8881 ggtgagcagg taaagaaacg caagtagtta accgctttag cggtagcatg taaattaaat
     8941 gcggttggca aacaattcaa tgagccttta ggctcaagca ggtatcatgc ccttataggg
     9001 caagtgcaaa ccacccgcta agcgggtggt catgatttgg tgggccttca gggactcgaa
     9061 ccccggacca accggttatg agccggttgc tctaaccaac tgagctaaag gcccgtttgg
     9121 ctcctcaagt tggactcgaa ccaacgaccc tgcggttaac agccgcatgc tc
//
LOCUS       DAOOJC010000033         9162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_6635_length_9162_cov_3.540134, whole genome shotgun sequence.
ACCESSION   DAOOJC010000033 DAOOJC010000000
VERSION     DAOOJC010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 9162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9162
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6635_length_9162_cov_3.540134"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="PLA01_01735"
     CDS             <1..1147
                     /locus_tag="PLA01_01735"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPD00053.1"
                     /translation="LKIIGKEIDDEYIARLKTLTVNPEISKEIGRSFKIVYTPLHGAG
                     NKPVRRILKETGFENVIVVKEQELPDPEFSTVEYPNPEERDAFKLAIELAKKENVDLI
                     IGTDPDSDRVGVVVRNKEGEYIVLTGNQTGCLLLEYILSQKKRRGELPENGFVVKTIV
                     TTELAREIASDYNVELIEVLTGFKFIGEKIKQLDEFGDMKYLFGFEESYGYLAGTFAR
                     DKDAVVASMLIAEMAAYYKSRDMTLYEGLLELFEKYGYTLEGISSFTLQGKEGIEKIK
                     SALESLRENKTVTFGVYEAITVRDYLTGERYSVASGQKEELTLPESDVLYYELDEGCW
                     FAIRPSGTEPKIKIYYGVKESTMDKAKEKLKHLQENVLSVIEKLLFD"
     gene            1249..4767
                     /locus_tag="PLA01_01740"
     CDS             1249..4767
                     /locus_tag="PLA01_01740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPD00054.1"
                     /translation="MQRFVHLHVHTEYSLLDGANRIKDLIKRTKELGMDSIAITDHGV
                     MYGVVDFYKEAVNNGIKPILGCEIYTTAGSRFDKKPGRDSDPGHMVLLAKNNVGYRNL
                     MKIVSIGFTEGFYYKPRVDMEVLEKYSEGLIASSACLSGDIPKAILNNNYKQAKEIAL
                     KLNSIFGQDNFYLELQINGIEEQNIVNQQLIKLGRETGIPLIATNDAHYLRREDARAH
                     EILLCIQTGKNINDEDRMMFSSDEFYIKSPEEMEEMFKNIPEAVSNTVRVAQMCNVEL
                     EFGKLHLPKFNVPQDREPYEYLRSLCYDGFERRYKEEERDEIKRNRLEYELSVIKQMG
                     YVDYFLIVGDFIRYAKEKGIMVGPGRGSAAGSLVAYCLEITNIDPIRYNLLFERFLNP
                     ERISMPDIDIDFCYERRQEVIDYVVEKYGEDRVAQIITFGTMAARAVIRDVGRALDMP
                     YGDVDAIAKMIPFQPGMNIDKALELNPELKQRYNEDDRVRELIDMARLLEGMPRHTST
                     HAAGVVISKDPLTEYVPLQKSEDSITTQFTMGLLEEMGLLKMDFLGLRTLTVIRDAVS
                     LIKKNYGKDIEIDELKMDDAKVFKLIGEGRTSGVFQLESAGMIQFMKELQPSSFEDII
                     AGISLYRPGPMDQIPRYIKNKRNPEYIKYDHPLLEGILDVTYGCMVYQEQVMQVVREL
                     AGYSMGRSDLVRRAMSKKKVNVMEQERKNFIHGIDDDKGNIIVIGAVRNGVNEETANK
                     IFDEMMDFASYAFNKSHAAAYAVVAYQTAWLKCYYPVEFMAALLNSFLGNSDKVSQYI
                     YECRKLGIEVLPPDINESDVRFTVVNGKIRFGMAAVKNVGENAVKTIIHERSNGGNFK
                     SLREFLERIDGKDVNKRCVESLIKSGAFDSMGIFRSKLIAVYEKMMDGISQTRKKNMA
                     GQLSLFDVSTNGGSENDNSTRGLFDDDNDQYPDIKEYPSKMILSMEKEMLGLYISGHP
                     LSEFEKEIAQQVNIFSSDLNTGQDESEEAIVNDESKNLYDGMNVTVGGIITSKKTKTT
                     KNNNLMAFLTLEDLYGTMEIIVFPSIFEKYSKLIEIENIVLISGRISIKEEEQPKIIC
                     EEVSPLRKTVSHKLYLKVNNDADSELMETVFCILKFFGGNTPVCMYDEHQKKVKVMER
                     DYWVSLNDVVLDELKTVLGDENVRVS"
     gene            4836..5069
                     /gene="mtrB"
                     /locus_tag="PLA01_01745"
     CDS             4836..5069
                     /gene="mtrB"
                     /locus_tag="PLA01_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp RNA-binding attenuation protein MtrB"
                     /protein_id="HPD00055.1"
                     /translation="MDWDIEKIFGDFVLIKAEENGVNIIGLTRGKDTKFHHTEKLDKG
                     EVLIAQFTDNTSAIKIRGKARIVCRHGEVYSGE"
     gene            5092..5289
                     /locus_tag="PLA01_01750"
     CDS             5092..5289
                     /locus_tag="PLA01_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00056.1"
                     /translation="MWTVVYMAQSKEIATQMQDFLANEGILVKLRPISKNHENNDNYF
                     EVLVPEAEVEEAHSVIIEKGY"
     gene            5490..6464
                     /gene="pfkA"
                     /locus_tag="PLA01_01755"
     CDS             5490..6464
                     /gene="pfkA"
                     /locus_tag="PLA01_01755"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPD00057.1"
                     /translation="MGGVKTIGVLTSGGDAPGMNAAIRAVVRTGLYYGFKVIGIRKGY
                     EGLIHGDIQEMTARSVGDIIHRGGTILQTARSPQFKTEEGLKKAMSMAKVFSIDAIVS
                     IGGDGSFRGARDLSKLGMNVIGIPGTIDNDIACTDYTIGYDTAMNTVQDAIDKIRDTA
                     YSHERCSVLEVMGRHAGYIAVNVSISGGAEAVVLPEKALDVDTDVIKPIIEGRNRGKK
                     HYLVIVAEGVEGKAIEIAKEITEKTGIEARATILGHIQRGGSPTVHDRVMASVMGAKA
                     IEILKEGKSNRIIAFKDNQVIDLDIDEALGMQKTLPEELIQLSKMLSL"
     gene            6583..6999
                     /locus_tag="PLA01_01760"
     CDS             6583..6999
                     /locus_tag="PLA01_01760"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPD00058.1"
                     /translation="MFISETEIIVRYAETDQMGIVHHSNYPVWFEAARTDFINKMGMS
                     YSQIEKSGFLLPLIELNCKYKGSARYEDRIVIRTRVKAISYSRLTFSYEVFRHGEDSL
                     LTSGETMHVWTDKGLRPLNIKKHAPDIYELLSKATQ"
     gene            7036..7578
                     /locus_tag="PLA01_01765"
     CDS             7036..7578
                     /locus_tag="PLA01_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPD00059.1"
                     /translation="MNIPNILTAIRLILIPFFGYYLFDKQYSVAVLLFLLGGITDVLD
                     GVIARKFNMVTSWGKLADPLADKLMQLTALSILTIQKIIPLPVLIIVVAKETFMVAGS
                     ILLYKKIKLVAQANWYGKLATVVFFIAIILTIAISSSGIVNPYTDALVNIFVIVAVVS
                     TLFAFFMYSREFKRITSKSK"
     gene            7882..8643
                     /locus_tag="PLA01_01770"
     CDS             7882..8643
                     /locus_tag="PLA01_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HPD00060.1"
                     /translation="MKRMADFFSYALVFCILITFSLTSVVSFAAQDEINVVLNGTKLE
                     FDVKPYIKNGRTMVPFRGIFEALGVEVSWDGVNRSVLATNDTTQIFIEIGKDYAFVNG
                     YRIDLDAAPEIINGRTFVPLRFVSENAGADVSWDGHTRTVYINYIDEKHEPGEIAYYR
                     ELEFTIDRVESEADGKLLKVYGRTNTAGKTLVIEVYDSSRRFSSGLAKVTKKEEEVYV
                     FQAEILVESSFEPKFIIVKTISDQKKLIKISQFDL"
     gene            8817..>9162
                     /locus_tag="PLA01_01775"
     CDS             8817..>9162
                     /locus_tag="PLA01_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030248 - cellulose binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose binding domain-containing protein"
                     /protein_id="HPD00061.1"
                     /translation="MTKRMLYKFSILLLNLSIFLSIISAASIGMNVYASGQNISVEFY
                     NGNTAQSADALSINFRIQNKDSSPIDLSDIRLRYYFNDDGISSISVAVDYAHNNYSMI
                     NSCIIYSINEVNS"
BASE COUNT         3014 a         1339 c         2204 g         2605 t
ORIGIN      
        1 gcttaagata atcggaaaag agattgatga tgagtacata gcaagactga agacccttac
       61 cgtgaatccc gaaatttcaa aggaaatcgg ccgcagcttt aaaattgtgt atacaccttt
      121 gcatggtgca ggtaataaac cggtaaggag aatacttaag gaaaccggat ttgaaaatgt
      181 aattgttgtt aaggaacagg agcttccgga ccctgagttt tccactgtag agtatcccaa
      241 tccggaggag agggatgcct ttaagcttgc tatcgaactg gcaaagaaag agaatgtgga
      301 tcttattatt gggactgacc cggatagtga cagggtcggt gttgtggtta gaaataagga
      361 aggagaatat atagttctga caggtaacca gacaggatgc ctcttgcttg agtacatatt
      421 gtcccagaaa aaaagaaggg gagagctgcc cgaaaatgga tttgtagtta agaccatagt
      481 tacaaccgag cttgccaggg aaattgccag cgattataac gttgagctta ttgaggtttt
      541 gaccggattt aagtttatcg gagaaaaaat caagcagttg gacgagtttg gtgatatgaa
      601 atacctgttt ggatttgaag aaagctatgg ttatctggcc ggtacctttg caagggataa
      661 ggatgctgtt gttgcttcga tgcttattgc ggagatggcg gcatattaca aatccaggga
      721 tatgacactg tacgaaggtt tgcttgaact gtttgaaaag tatgggtata ctcttgaagg
      781 aatttcttca tttaccctcc aggggaaaga aggtatagaa aaaataaagt cggctctgga
      841 aagccttaga gaaaataaaa ctgtaacgtt tggagtatat gaggcaatta cggtaaggga
      901 ttatttgacg ggtgagaggt attcggttgc ttcggggcaa aaagaggaac tgacgctgcc
      961 tgaatccgat gtattatatt atgagttgga tgaaggatgt tggtttgcta taaggccttc
     1021 gggtacagag cctaaaatta aaatttatta tggtgtaaaa gagagtacta tggataaggc
     1081 taaggaaaaa cttaagcact tgcaggaaaa tgtactttct gtgattgaaa aactgctttt
     1141 tgattaatat ttttctttac attattaaaa acccacgacg gagtattaat gtacttcgtt
     1201 tttttgttat gttataataa aaagcaaaag ctacaaaggg tgatataagt gcaaaggttt
     1261 gtacacctcc acgttcatac ggaatacagc ctgctggacg gtgcaaacag gataaaagac
     1321 ctgataaaac gcacaaaaga gctgggaatg gacagtatag ccataacaga ccatggtgtt
     1381 atgtatggag tggttgattt ttataaagaa gcagtaaata acggtattaa acctattctt
     1441 gggtgcgaaa tatatactac ggcaggatcg aggtttgaca aaaagcccgg gcgggattcg
     1501 gatcctggtc atatggttct tttggctaaa aacaacgtag gatacaggaa tttgatgaaa
     1561 atcgtttcca taggttttac cgaagggttc tactataagc cgagggttga catggaggtg
     1621 ctcgaaaaat acagtgaagg actgattgca tctagtgcct gcctttccgg ggatataccc
     1681 aaggcaattt taaataacaa ttacaagcag gcaaaagaga ttgcattgaa attgaacagc
     1741 attttcggac aggataattt ctaccttgaa cttcagataa acggcataga agagcagaac
     1801 attgtcaacc aacagcttat aaagcttgga agagagaccg gcataccgct tattgcgacc
     1861 aacgatgctc attatttacg ccgggaggat gcgagggccc atgagatctt gctttgtatt
     1921 cagaccggaa aaaacataaa tgacgaagat cgcatgatgt tttcttccga tgaattctat
     1981 atcaaatctc ccgaagaaat ggaggagatg tttaaaaaca tacccgaagc tgtctctaat
     2041 acagttaggg ttgcacaaat gtgcaatgtc gagttggaat ttggtaaatt gcatctccca
     2101 aagttcaatg tgcctcaaga cagggaacct tacgaatatt taaggtccct ttgttatgat
     2161 gggtttgaaa gaagatacaa agaagaggaa agggacgaga ttaagagaaa caggcttgag
     2221 tatgagctgt ctgttataaa gcaaatgggt tatgtggatt acttccttat tgtaggggat
     2281 tttattaggt atgccaaaga aaaaggaata atggtaggtc ccggaagggg ttcggcagcg
     2341 ggaagccttg tggcttactg ccttgaaatc acaaatattg atccaattag gtacaacttg
     2401 ctttttgaaa ggtttctaaa tcccgaaagg ataagtatgc cggatatcga catagacttc
     2461 tgctatgaaa gaaggcaaga ggtaatagat tatgtagtgg aaaagtatgg agaagacagg
     2521 gttgcacaga taattacatt tggtacgatg gcggcgaggg ctgtaataag agatgtgggc
     2581 agggctcttg atatgcccta tggagatgtg gatgctattg caaaaatgat tccctttcag
     2641 cccggtatga acattgacaa ggctttagaa ctcaatcccg aattaaagca gcggtacaat
     2701 gaagacgaca gggtaaggga attgatagat atggccagat tgcttgaagg aatgccaaga
     2761 catacttcca ctcatgcggc cggagtagtt atatccaaag atccccttac ggaatatgtt
     2821 cctttacaaa agagtgagga cagcataaca actcagttta ccatggggct tttggaggaa
     2881 atgggtcttc ttaaaatgga ttttttaggc ttacgaaccc taacggtaat aagggatgca
     2941 gtaagtctta ttaaaaagaa ttacggtaag gatattgaaa ttgacgagct taaaatggat
     3001 gatgcaaaag tgtttaagct tatcggagag ggtagaacct caggggtgtt tcaattggaa
     3061 agtgcaggaa tgattcaatt tatgaaggag cttcagcctt catcttttga agatataata
     3121 gcaggtattt cgctataccg cccgggacca atggatcaga ttccaagata tattaaaaat
     3181 aaacggaatc ccgaatatat aaaatatgat catcccctct tggagggtat attggatgtt
     3241 acttacggct gcatggttta tcaggaacag gttatgcagg tggttcgtga acttgcgggc
     3301 tattccatgg gaaggtctga tttagtaaga cgagcgatgt ccaaaaaaaa ggtcaatgta
     3361 atggaacagg aaagaaaaaa ttttattcat ggaatagatg acgataaggg aaatattata
     3421 gtaatagggg ctgtcagaaa cggcgtaaat gaagagactg caaacaagat atttgacgag
     3481 atgatggatt ttgccagtta tgctttcaac aagtctcatg cggcagcata tgccgttgtc
     3541 gcttatcaga ctgcatggct gaagtgctat tatcccgttg agtttatggc ggcattatta
     3601 aacagctttt tgggaaacag cgataaggta tcccagtata tatatgagtg caggaagctt
     3661 ggaattgaag tattgcctcc cgacattaac gagagcgatg ttaggtttac tgttgttaac
     3721 gggaaaataa ggtttggtat ggctgcggtt aaaaacgtcg gagagaatgc tgttaaaaca
     3781 attattcacg aaagaagcaa tgggggaaac tttaaaagct taagagaatt ccttgaaaga
     3841 attgacggaa aggatgttaa caaaaggtgc gttgaaagcc ttataaaaag cggagccttt
     3901 gattctatgg gtatattcag gtcgaagctt attgctgtat atgaaaagat gatggacggc
     3961 atatcccaga caaggaagaa gaacatggcg gggcagcttt cgttatttga tgtgtcaact
     4021 aacgggggca gcgaaaacga taacagcaca aggggacttt ttgacgatga taacgaccag
     4081 tatcccgata ttaaggaata tcccagcaag atgattttat ccatggagaa ggaaatgttg
     4141 ggactatata tatccgggca tcctttgagc gaatttgaaa aggaaattgc acagcaggta
     4201 aatatattca gttccgattt gaataccgga caggatgagt cggaagaagc tattgtcaac
     4261 gatgaatcga aaaatttata cgatggtatg aatgttactg ttggcggaat aattacatca
     4321 aaaaaaacaa aaactacaaa gaataataat ttgatggctt ttttaactct ggaggactta
     4381 tacgggacga tggaaattat tgtttttccg tcgatatttg aaaagtattc aaagcttata
     4441 gaaattgaaa atattgtttt aattagtgga cggataagta taaaagaaga ggaacagcca
     4501 aaaataatat gcgaagaagt tagtccgttg agaaagacag tttcgcacaa actatattta
     4561 aaagtgaaca atgatgcgga cagtgaactg atggaaacgg ttttttgcat attgaagttt
     4621 tttggaggaa acactccggt gtgcatgtat gacgagcatc aaaagaaggt taaggtaatg
     4681 gaaagagatt attgggtaag ccttaatgac gtggttttag acgaattaaa aacagtgctt
     4741 ggggatgaaa atgtaagggt ttcataagtt gttgcccaaa ttccatattg atttttgttt
     4801 aaaattaata tatagttatg tcgaggtggt tttagttgga ttgggatatt gagaagatat
     4861 ttggagattt tgtcttaata aaggctgagg aaaatggagt aaatattatt ggtcttacca
     4921 ggggcaagga tacgaagttc catcatactg aaaagctgga taagggtgaa gttcttatag
     4981 cccagtttac cgataatact tctgctataa aaattagggg gaaagctaga atagtttgtc
     5041 ggcatggaga agtgtattcc ggtgaataac tgaaaaaggg gcgtgtttta tatgtggaca
     5101 gtggtgtata tggctcagag caaggagatt gccactcaga tgcaggattt tttggcgaat
     5161 gaaggtattc tggtaaagtt aagacctatt agcaagaatc atgagaataa tgataactat
     5221 tttgaggttc tggtaccgga agctgaagtt gaagaggcac atagcgtgat tattgaaaag
     5281 ggatattaaa aaactggtta gttaaatgct tttttgataa ataagtttag tacgataggg
     5341 actatcggat attaagcctg tggagattgc aaggtctggg aagcaggaat cctgtcatat
     5401 tagtggtggg aagttcaata tcccgtgttt aattaaaaac acgggtttaa tattatatgc
     5461 gggtttataa tacattaagg aggccaatta tgggtggtgt aaaaacaatt ggtgttttaa
     5521 caagcggcgg agatgcaccg ggaatgaatg cagccataag agcggtggtt agaacaggac
     5581 tttactatgg atttaaggta attggaataa gaaagggtta tgaaggactt atccatggag
     5641 atattcaaga aatgacggca aggtctgtcg gagatataat ccatagagga ggaactattt
     5701 tacagacagc ccggtcaccg cagtttaaaa cagaggaagg tttaaaaaag gccatgtcta
     5761 tggcgaaggt ttttagtatc gatgcaatcg tttcgattgg cggtgacggt tctttcaggg
     5821 gagccagaga cttaagcaag ctggggatga atgttatagg gattcccggg acgattgata
     5881 atgatattgc ctgtacggac tacactattg gttatgatac agccatgaat actgttcagg
     5941 atgcaataga taaaattcgg gatacagctt attcccatga gagatgcagc gtgcttgagg
     6001 ttatgggaag acatgccgga tatattgccg taaatgtcag tatatcggga ggagcagagg
     6061 cagttgtgtt gcctgaaaaa gctttagatg tggatacaga tgtaattaag cctataattg
     6121 agggtagaaa caggggtaaa aaacactatc tcgtcatagt tgccgaaggt gttgaaggaa
     6181 aagcaattga gatagcgaag gagattacag aaaaaactgg cattgaggcc agagcaacaa
     6241 tactgggaca tattcagcgc ggaggaagtc ctactgttca tgacagggta atggcaagtg
     6301 ttatgggagc caaggccatt gagattctaa aagaaggcaa aagtaacaga ataatagcat
     6361 ttaaagacaa tcaggtaata gatctggata ttgacgaggc attaggaatg cagaaaacct
     6421 tgccggaaga attgattcag cttagtaaaa tgttgtcgtt atgatgtaag aaaattttct
     6481 tagaacgaga aatcttttaa actgaagtgt tataatgagg ttgaatgctt gcttactcaa
     6541 gcggcggatt gtccttactt cgttgaaatg caggtggaaa cagtgtttat atcggaaaca
     6601 gaaattattg taaggtatgc tgaaaccgat cagatgggaa ttgttcatca ttccaattat
     6661 cccgtctggt ttgaggcagc aagaacagat tttatcaata aaatgggaat gtcctattct
     6721 caaatagaaa aaagtggctt tttactacct ttgattgagc ttaactgtaa atataaaggg
     6781 tctgcaaggt atgaggatag gatagtaata agaaccagag tgaaggcaat atcatactcg
     6841 aggcttacat tttcttacga agtcttcaga cacggtgagg attcattatt aacctcgggt
     6901 gagacgatgc atgtatggac agacaaaggg cttaggcctt tgaacataaa aaaacatgct
     6961 ccggatattt atgaattatt gtccaaggca actcaataaa gtgggattta aatattttta
     7021 acaggtggtg gctgagtgaa cattcctaat atattaactg ctataaggct catactgatt
     7081 ccgttttttg ggtattacct tttcgacaag cagtattctg ttgcggtttt attgttttta
     7141 ctcggaggaa ttaccgacgt tttggatggg gtaattgccc ggaagtttaa tatggtaacg
     7201 tcatggggga agctggctga tcctctggca gacaaactaa tgcaactgac ggcactgtca
     7261 attctcacaa tacagaaaat tataccgttg ccggtattga taattgttgt tgcaaaagaa
     7321 acgttcatgg ttgctggaag tattttactg tataagaaaa taaagctggt tgcacaggct
     7381 aattggtatg gaaagcttgc taccgttgtt ttctttattg caataatttt gactatagct
     7441 ataagttcaa gtggaattgt gaatccttat accgatgcat tggttaatat ttttgtcata
     7501 gttgctgttg tctcaacact ttttgctttt tttatgtatt caagggagtt taagagaatt
     7561 acatcaaaat caaagtaaat tgtaccggga aatatatcaa aagcacgtaa ttaattacag
     7621 gtatttactg gattacatag cttcaggagt aataatatat caaatatatt acaaagtttt
     7681 tctaaaagtt gacctgggta ttgaatttgt tatataatag tattgtgaag tatgttgcat
     7741 taatttattt actttcttta ttttgcagct gaagtatgca taactgcaat caattttaag
     7801 ttctactttc cttgctaaga catattatct tagtgttttt tattcctatt tattattatg
     7861 aaaaggtaga ggagaatggt tatgaagaga atggccgatt ttttttcata tgcattggtg
     7921 ttttgtatat tgataacttt tagtttaaca agcgttgttt cctttgcagc tcaagatgaa
     7981 ataaacgtcg ttttaaacgg aacaaagctt gagtttgatg taaaacctta tataaaaaat
     8041 ggaagaacaa tggtaccttt caggggcata tttgaagccc tgggggttga ggtaagctgg
     8101 gatggagtga accgcagcgt attggcaaca aatgatacaa cgcagatttt tattgaaatc
     8161 gggaaggact atgcctttgt taacgggtac cggatagacc ttgatgcggc tcctgagatt
     8221 attaacggaa ggacttttgt gcctttaaga tttgttagcg agaacgccgg tgcagatgta
     8281 tcatgggatg ggcacacaag aacggtttat atcaattata tcgatgagaa gcatgaacct
     8341 ggagaaatag cctactatag agaattagag tttacaattg acagagtcga aagtgaagct
     8401 gacgggaagc tgctgaaagt atacggcaga acaaacacag caggtaagac actggtaata
     8461 gaggtgtatg atagttcgcg gagattttcc agtggtttgg cgaaggtgac gaaaaaagaa
     8521 gaggaagtgt atgtatttca ggctgaaata ttagtggaat cctcttttga acctaagttc
     8581 attattgtaa aaaccatcag tgaccaaaag aagctgatta aaatctctca gtttgactta
     8641 tagtttttgg gattaacttt gcaaacaaaa aacttaataa taaccagtca tacataaaca
     8701 actgcacact gataaccgaa cattgacaac aaaaccatac aaaaattgca gcaaatcatt
     8761 aaccaaacat tatactaacg tcaaccaaac acgaaattat aaggaggtag aatcctatga
     8821 cgaagagaat gctctacaaa ttcagcatac tactacttaa tttaagtata tttttaagta
     8881 ttatttcggc agcaagcatt ggtatgaatg tgtatgcaag tggacagaat atcagcgttg
     8941 agttttacaa cggtaacaca gctcagtctg cagatgcatt atctatcaac tttagaatcc
     9001 agaataaaga ctcttctccg atcgatcttt cagacatcag actcaggtat tattttaatg
     9061 atgacggtat atcgtctata agtgttgctg tcgattatgc acacaacaac tatagtatga
     9121 ttaacagctg tataatatac tccattaatg aagtaaattc ac
//
LOCUS       DAOOJC010000034         9033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_6739_length_9033_cov_3.180887, whole genome shotgun sequence.
ACCESSION   DAOOJC010000034 DAOOJC010000000
VERSION     DAOOJC010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 9033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9033
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6739_length_9033_cov_3.180887"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /gene="hydF"
                     /locus_tag="PLA01_01780"
     CDS             <1..1131
                     /gene="hydF"
                     /locus_tag="PLA01_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HPD00062.1"
                     /translation="AVVSEIAGTTTDPVQKTMEILPIGPVVVIDTAGYDDTGDLGNLR
                     VEKTYDVLRKTDLAIVVISAEDGITDDDRDFIKGVKRKEIPTIVVINKSDKSDINEEQ
                     LNIYKEELGVPLCKISAENNLGIDELRDLIVKNAKYEEMQTTIVGDLIKPGDMVVLVT
                     PIDLSAPKGRLILPQQQTIRDILDNDAIAVVTKENGLRDTLNNLSKKPSIVITDSQVF
                     SKVAAETPEDIPLTSFSILFARYKGELRQMVMGIRKIKELKAGSRVLIVEGCTHKCEA
                     DDIGRVKIPGWLRKFCGEEIEFEWANGTFYPKEIDKYDLIIHCGGCMLNKKEMCYRID
                     AAKESNVAITNYGMIIAYVHGILPRALQPFPTVREMIEFLAP"
     gene            1156..2301
                     /locus_tag="PLA01_01785"
     CDS             1156..2301
                     /locus_tag="PLA01_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPD00063.1"
                     /translation="MIYLDNASTSFPKPERVYDEMIRCMKEYCANPGRGGHDLSIQSG
                     RAVFEAREAVARFFNIDNPMQIVFTKNATEALNIAIKGVLQKGDHVVTTSMEHNSVIR
                     PLKALESDDVITLTIVKGNEFGEIDPEDIRRSIKGNTKLIVCTLSSNVNGIIMPLAQI
                     GRIAEEKGVLFLVDASQGAGSIKLDVNQIRVDMLAFPGHKGLLGSQGTGGLYVNEKVK
                     IKPIMQGGTGSNSEKYHQPEFYPDLLESGTLNTPGLIGLRHGIGFIESFGIDNVNIYK
                     HMLTKKLYEGIADIKGIKVYSKPDKEKNSGIIAFNITGIESSQVCFILDRNYGIASRA
                     GLHCAPLAHETIGTKSTGTVRLSVGCFNCVEEIDITVNSLIEISKAV"
     gene            2582..3688
                     /gene="pilM"
                     /locus_tag="PLA01_01790"
     CDS             2582..3688
                     /gene="pilM"
                     /locus_tag="PLA01_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HPD00064.1"
                     /translation="MLMDLLYKKTSMVCIDVGYRNIKVVEVSVRKNNSIFINNYGIVS
                     TPTDCIKNGAIYDVDKVLSVVKAVIKEQNMKAKNAKIIMSGTNIITRIYMIDKVTGES
                     DDFTVKNSIPKFLPIDVDNYRMDYKILNTIKEKGKEKYKVFVTAVPKNILQSYVDVLQ
                     GLDLKPLAVDIPANSTAKFFNREIQTKDMDDYYSKRKFKKVESDTFAVLDFGSETTIV
                     NFLKDRVLEFNKVILSGSANMDEHISRELDIPLQEAERLKKLYGMTTPNNLSKRDHVV
                     TYGKVSGFIERLTKQIAKCFEFYLERCYGTPISKIFIIGGGSQLSGLNQYLFSTFNVP
                     VYPVGLLNMKGVELKKSLDRDKLNYLINSVGISL"
     gene            3886..4056
                     /locus_tag="PLA01_01795"
     CDS             3886..4056
                     /locus_tag="PLA01_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00065.1"
                     /translation="MDNRLVRSIITIVIAVILLIVAVRFLYWAAFKLLPVAIVIIAAY
                     IVYRIVTGKKAF"
     gene            complement(4169..4762)
                     /locus_tag="PLA01_01800"
     CDS             complement(4169..4762)
                     /locus_tag="PLA01_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00066.1"
                     /translation="MKNRITKEDIAQLTDAQKKRLNELWLPEKYDVALANICVNAETE
                     EYAQIEFVIGDISLYNTHIIVHDLLFLNDAKSSKDGSHDDSDGSYKSDDTEADFPDDD
                     YDSEYIPEEDDDFEFSFMRPENYSKEDCIPLLSIGQMIGILCNNSSKSFDFFLLADSG
                     EFGVQLGSQLYNLSGYGSDFDSKELCDVLFECVKKIL"
     gene            5017..5568
                     /locus_tag="PLA01_01805"
     CDS             5017..5568
                     /locus_tag="PLA01_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HPD00067.1"
                     /translation="MKCPICKNVCLKKTNLMDDLFAFACDTCGGNWMRYEDYWKWYCN
                     NTEIKVDWNNIKKHLAVSDSSKAKICPDCGRILIKYKVDNRLDFYLDQCGCCNGIWFD
                     KNEWDNLKLNNIHNRIHHFFTKPWQKKLREEAARDKFEDKYIKKFGRWDYERLKEMRE
                     WIYNSSTKSEMLSFLIDEDPYKL"
     gene            complement(5602..7110)
                     /locus_tag="PLA01_01810"
     CDS             complement(5602..7110)
                     /locus_tag="PLA01_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HPD00068.1"
                     /translation="MPKYVNQHRFLLLLTICVILMTGFLLSGCTNDPPISSHKPDSDL
                     PSAIDTEGNKPLNIDKDSFAGTNGKLQVIGNQLCNEKKEPIQLKGISSMQLHMFGRFV
                     NKDTIKWLRDDWSITVFRAAMYTEEGGYIQNKSVKEKVKEAVEASIELGIYVIIDWHI
                     LSDGDPNEHIDEAKEFFREMSALYRHYPNVIYEICNEPNGDNVNWTNSIKPYAQEIIP
                     IIREASPDSIIIVGTSHYSQNVDEAADDPLEFNNILYAMHFYAGTHETSLRDKTNYAL
                     NKGAAIFVSEWGTSSSTGSDGVFLKESIEWLEYLDSNKISWVNWSLCDKNESSAALTF
                     TAGTKGNWSDNDLTESGLFVKYTLLGKSHYTLFSDGFETGIFTKGLISDGCTISNDTT
                     YKGKFCVSFKNGGSLTKELSTSRFSDIEVYFAYRTIGFTDNDFLIVEGFDGSDWMELG
                     KYSVSNDWTEKSIILPQTASDNENFKIRFSTNTDTQSSIVYLDEFKITAKKE"
     gene            7335..7598
                     /locus_tag="PLA01_01815"
     CDS             7335..7598
                     /locus_tag="PLA01_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00069.1"
                     /translation="MNSVELFEYLKKISDYIEYSFYKDSSEIMQAILNDGFEDVLSIL
                     SIKEIKKGLYQCNTSWKIAPDQDISKNVNIDITDEMIYITEVI"
     gene            7929..>9033
                     /locus_tag="PLA01_01820"
     CDS             7929..>9033
                     /locus_tag="PLA01_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HPD00070.1"
                     /translation="MLVKRRILSVIVFIIVSILIIKLPLSVSAAQVKAGSGSYTDVLP
                     SGCISPQSTIYKTSNLKGAIPTSSWESSILWYQYSLPIFAHPLTFCFKPEGLEIGNPA
                     LGGGGIAYFGPHKNDFSIGHSSIGVFPDVQADKISDFAVDAVMYSGNAGFKSTIVKGS
                     PYAYFAFTGGNPVISFFSAPVLFYGNWDTQCLGVTINGVNYGLFAPTGSTWQGIGTGT
                     VTCILPSGKNYFSIAVLPDNSIQTLLYYKERAYSFVTDTKVNWEYDEENSRVTTTFSV
                     ETTPMEGTNKNTIFALYPHQWRNNPHISTLPYSYSTLRGIMKTTEGTSFKTEYKYQGI
                     LPSLPDKGTYDKSILNSYINELTAQANSPQSQDT"
BASE COUNT         2964 a         1460 c         1844 g         2765 t
ORIGIN      
        1 gcagtggttt ctgaaattgc agggacaaca accgacccgg tgcaaaagac tatggaaata
       61 ttgcccatag gaccggtggt tgttattgat acggcaggct atgatgatac gggcgattta
      121 ggaaatttga gggttgaaaa gacctatgat gttttaagaa agacagatct agcaattgtt
      181 gttattagtg ctgaggacgg aataacagat gatgacaggg attttattaa aggtgttaaa
      241 agaaaagaaa tcccaacaat agttgtgata aataaatcgg ataaaagtga tattaatgaa
      301 gagcaactga atatatataa agaggaactg ggtgttccgc tttgtaaaat aagtgcggaa
      361 aataacttag ggattgatga gttaagggat ttaattgtta agaatgcaaa atatgaagaa
      421 atgcagacta ccatcgttgg cgatcttata aagcccgggg atatggttgt tcttgtaaca
      481 cccatagatt tgtcagcacc aaagggacgg ctgattcttc cgcagcaaca gacaatcagg
      541 gatatacttg ataacgatgc tattgctgtt gttacaaagg aaaatggatt aagggatact
      601 ttgaataatc tatcaaaaaa gccctcaatt gtgataactg attcacaggt gttttcaaag
      661 gttgcggccg aaacaccgga ggatattcct cttacgtcgt tctctatatt gtttgcccgg
      721 tataaagggg aactcaggca gatggtgatg ggtatcagaa aaattaaaga attgaaagca
      781 ggaagcagag ttttgatagt tgagggctgt acacataaat gtgaggccga tgatatagga
      841 agggtaaaga ttccgggatg gttaaggaag ttttgcggtg aggaaataga gtttgagtgg
      901 gctaacggaa ctttttatcc gaaagaaata gacaagtatg atttgattat tcactgtggc
      961 ggttgtatgc tcaataaaaa ggaaatgtgc tacaggattg acgctgcaaa agagagtaac
     1021 gtggcaataa ccaattacgg tatgattata gcatatgttc atggaatact gccaagggct
     1081 ttacagcctt ttcctacagt cagggagatg attgaatttt tggctccctg agaataaaat
     1141 acagggagga gtgcaatgat atatcttgac aacgcatcaa cttcgtttcc gaagcctgaa
     1201 agagtttatg atgagatgat acggtgtatg aaggaatact gtgcaaaccc cggaaggggc
     1261 ggacatgatt tgtcaataca gtccgggagg gcagtgtttg aagcccgtga agcagtggca
     1321 cggtttttta acatagataa ccctatgcag atagtgttta caaaaaatgc aacagaggcc
     1381 ttgaatattg cgataaaagg agtgcttcaa aagggagatc atgttgttac cacaagcatg
     1441 gagcacaact ctgttataag acctcttaag gcccttgaga gtgatgatgt tataacactt
     1501 acaatagtta agggaaatga atttggggaa attgatcctg aggacataag aagaagtatc
     1561 aagggaaata ctaagctaat cgtatgtacg ctttcatcga acgttaacgg aattattatg
     1621 ccattggcac aaataggccg gatagcggag gagaaaggtg tcctgtttct ggttgatgct
     1681 tcacaggggg caggttccat aaaactggat gttaatcaga tacgggttga tatgcttgcg
     1741 tttcccggtc ataagggctt gcttggttcc cagggcacag gaggtctcta tgtcaatgaa
     1801 aaggtaaaaa ttaagcctat tatgcaaggc gggacgggaa gcaactccga aaaataccat
     1861 cagccggagt tttaccccga tttgctggaa agcggaactt taaatacacc gggtttgata
     1921 ggcttaaggc atggaatagg gtttattgaa agtttcggta tagataacgt gaacatatac
     1981 aaacacatgc tcacaaagaa gctttatgaa ggcattgcag acataaaagg aattaaggtt
     2041 tatagtaagc cggataaaga aaaaaattcc ggtattattg cctttaatat aacaggcatt
     2101 gaatcttcac aggtttgctt tatccttgac agaaattatg ggattgcttc ccgtgcggga
     2161 ttgcattgtg ccccactggc acatgaaacc ataggaacaa aatcgaccgg gactgtcagg
     2221 ttaagcgttg gatgctttaa ttgtgttgag gaaatagata taacagttaa ttcattaata
     2281 gaaatatcaa aggcagtata aatgaggata tataaaaaaa ttaaataatt ttgatgttta
     2341 tggcataatt acaacataat aaaaatataa atacaaaaat ataaaggtat taataataga
     2401 tgctggaaat ctaaaaattg ttgtgctata ataatttagc atacatattt ttttagggcg
     2461 aactcctatt atgtaaatta atttgtttat ataattggta ttatacccca taatatatac
     2521 tgtatatgca taatctatca tgaacatgat acgttcactt ataaggggga tggatggata
     2581 aatgctaatg gatttattgt ataaaaagac ttccatggta tgtattgacg ttggatatag
     2641 gaatataaag gtggttgaag tatccgtaag aaagaataat agtattttta ttaataatta
     2701 cggcattgtt tctaccccca cagactgtat taagaacggt gccatatatg atgtcgataa
     2761 ggtgcttagt gttgttaaag cggttattaa agaacagaac atgaaagcaa aaaatgcaaa
     2821 aataataatg tccgggacca atatcattac aaggatttat atgattgaca aggtaaccgg
     2881 agaaagtgac gacttcactg taaaaaacag tattcctaaa tttctgccca ttgatgtaga
     2941 caactacagg atggattata aaattttgaa tacaattaaa gagaagggaa aggaaaaata
     3001 caaggttttt gttactgcgg ttcccaaaaa tatcctgcag agttatgttg acgtactgca
     3061 aggtttagat cttaaacctt tagctgttga catacctgca aacagtactg ccaagttttt
     3121 taacagggaa attcagacaa aggatatgga tgattattat tcaaaaagga agttcaagaa
     3181 agtagaaagt gatacttttg cagtgctgga tttcggatcc gagacgacaa ttgtaaactt
     3241 tctcaaggat agggttctcg aattcaataa agtaattctg tccggaagtg ccaatatgga
     3301 cgagcacatt tcaagagagc ttgatattcc tttacaggag gcagaaagac tcaaaaaatt
     3361 gtatggaatg actacaccga ataatctttc caaaagggat catgttgtaa cttatggaaa
     3421 ggtcagcgga tttattgaaa gattgacaaa gcagattgca aagtgttttg agttttatct
     3481 ggaaaggtgc tatggtacac ctatttccaa gatttttata attggcggag gttcacagct
     3541 tagcggattg aaccaatacc ttttctccac ttttaatgta ccggtttatc ctgtgggctt
     3601 gttaaacatg aaaggcgttg agctgaaaaa atctcttgac cgggataaac tgaattattt
     3661 gattaactct gttggaatat cgctataaat aaattataaa atattgtatg aaatgtatat
     3721 gaaaaataaa aaagagctac cctggttaaa accggggttt ttatatttaa gtttattatt
     3781 atgtttaaat aaatctatgc atgtattaaa aaaatcaatt atttattaga caattattaa
     3841 tttatatcgt ataatatata aagaatatta attgaggtga tgcgaatgga taatcgttta
     3901 gtgaggtcaa taattacaat agtgattgct gttatattat taattgttgc tgttagattt
     3961 ctttattggg cggctttcaa gcttctaccc gttgcaattg ttataattgc tgcatacata
     4021 gtataccgca ttgttacagg aaagaaagct ttttagataa ttcgcagaat tctttttcaa
     4081 aaagggcagt gggggcattt taatcgggcc cccattgctt ttatgttcat ttcacgtcga
     4141 gaaatcccgt aaataatata aaaacaaatt ataaaatttt ctttacacat tcgaagagaa
     4201 catcacaaag ctcttttgaa tcgaaatcgc tcccataacc ggaaaggtta tatagctgac
     4261 ttcctaattg cacgccgaat tcgccgctat ctgccaagag aaaaaagtcg aaggattttg
     4321 aagaattgtt gcaaagtatt ccgatcatct ggcctatgct caacagagga atacaatctt
     4381 ctttactgta gttttcaggc cgcataaatg aaaactcaaa gtcgtcatcc tcttccggaa
     4441 tgtattcgct atcgtaatca tcatcaggga agtctgcttc agtatcgtcg gacttataac
     4501 tgccatcact gtcatcatgg gatccgtctt tagagctttt agcatcattt aaaaacaata
     4561 agtcatgcac aattatatgt gtgttataaa gcgagatatc gcctataaca aactcaatct
     4621 gtgcatattc ctcggtttca gcatttacgc atatgttggc aagggcaaca tcatattttt
     4681 caggaagcca taattcattc agcctctttt tttgggcatc agtaagctgt gctatatctt
     4741 cttttgttat acggtttttc atttattatc attccttcca ccagattctt gattcatata
     4801 atatcatatt atatggatta gtcaattata ttaactccca aggaatgcat tatataattg
     4861 tgaagataat tattctgcag gcttaagttg gcaagttaag agcttgtaaa taatatacac
     4921 acactcacag gtttctttat aaatgtgatt ctttactctt aattgagaat atttttgtac
     4981 aagtactaat aaaaatatat gggaggtgtg attgatatga aatgtcccat atgtaaaaat
     5041 gtttgtctta aaaaaacaaa cctgatggat gatttatttg ctttcgcctg tgacacttgc
     5101 ggcggaaact ggatgagata tgaggattat tggaaatggt attgcaacaa tacggagata
     5161 aaagttgatt ggaacaacat aaaaaaacat ctggctgttt ctgattcatc aaaggcaaaa
     5221 atatgcccgg attgtgggag aatattgata aaatacaagg tcgacaacag gctggatttc
     5281 tatttggatc aatgcggatg ttgcaatgga atatggtttg acaaaaatga gtgggacaat
     5341 ctgaaactga ataatattca taaccgtata caccactttt tcacaaagcc gtggcagaaa
     5401 aagctgcgtg aagaagcggc acgggataaa tttgaggata aatatataaa aaagttcggg
     5461 cgttgggatt atgaaagact gaaggagatg agagagtgga tatataatag ctcaacaaaa
     5521 agtgaaatgt tatcttttct tattgatgaa gacccttata agctttagat gtcaaaattc
     5581 cctcaagtat gtacttaaac cctactcttt cttagccgtt attttaaatt catcaaggta
     5641 aacaattgat gattgagtat cggtattggt tgaaaacctt attttaaaat tttcgttgtc
     5701 tgaagcggtt tgcggaagga taatgctttt ttcggtccaa tcattgctga ccgaatattt
     5761 tcccagttcc atccaatcgg atccgtcgaa cccttcaacg ataagaaaat cattatctgt
     5821 aaacccgatg gtgcggtaag caaagtatac ctcaatatcg gaaaatctgg atgtgctcaa
     5881 ttctttagta agggagcctc cattcttgaa tgaaacacag aattttcctt tatatgtggt
     5941 gtcattgcta atggtacaac cgtctgaaat caaccccttt gtaaatatgc cagtttcgaa
     6001 tccgtcacta aaaagggtgt agtgtgactt gccaagaagg gtatacttaa caaataaacc
     6061 cgattcagtc aggtcattat ctgaccagtt accttttgtt ccggcagtaa aagttaatgc
     6121 cgcggacgat tcatttttat cacaaagtga ccaattgacc cagctgatct tattgctatc
     6181 gaggtattcc agccattcta tcgattcttt aaggaaaaca ccatctgaac cggttgatga
     6241 ggatgttccc cattcactga caaaaatagc ggcaccttta ttcaatgcat agtttgtttt
     6301 gtctctaagg gaagtttcat gggttcctgc atagaaatgc atagcataca agatgttatt
     6361 gaactccaga ggatcatcgg cagcctcatc aacattttgg ctgtagtgtg atgttccaac
     6421 aataattatg ctatcgggag aggcttctct tattatagga atgatttcct gtgcataggg
     6481 tttaatactg ttggtccaat taacattatc gccgttgggt tcgttgcaaa tctcatatat
     6541 gacatttggg taatgcctgt ataacgccga catttctctg aaaaactctt ttgcttcgtc
     6601 aatatgctca ttaggatctc cgtcacttaa aatatgccag tcaattataa catatatacc
     6661 aagctctatg gaggcttcca cagcttcctt aaccttttcc ttaacagatt tgttttggat
     6721 gtatccgcct tcctcagtat acattgctgc cctgaaaact gtaatactcc aatcgtctct
     6781 gagccacttt atagtatcct tgttaacgaa gcgtccaaac atatgaagct gcattgagct
     6841 tattcctttt agttgaatgg gttctttttt ttcattacac agctgattac caattacttg
     6901 taatttgccg tttgtaccgg caaatgaatc tttatctata ttaagcggtt tattgccttc
     6961 agtgtctatt gctgaaggca aatcagaatc aggtttgtga gaagaaattg gcggatcgtt
     7021 ggtgcatccc gataataaaa aacctgtcat caatatgacg catatagtaa gaagcaataa
     7081 gaacctgtgt tggttaacat attttggcat tgcgttaccc ccagcaaatg tatgaacctt
     7141 tttaaagtta cataatatga ttataacaga atactgccta caatttcaat agacgcaatt
     7201 gcttagaata ataatacaat taaggataaa tatatcgaat aatattaaat tcataataat
     7261 tatatgataa tcgatttaat ataatacatt cgttacaaac acccgataag tataaaaaga
     7321 aggaggaata aatcatgaat tctgtggaac tgtttgaata cctgaaaaag atttccgatt
     7381 atattgaata ttctttttac aaggattctt cagaaataat gcaggctatt ctaaacgacg
     7441 gatttgaaga cgtattgagt atcttatcca ttaaagaaat aaaaaagggt ttgtatcaat
     7501 gcaatacatc atggaagata gcaccggatc aggatataag caaaaacgtg aatatcgata
     7561 ttacagatga aatgatttat attaccgagg tcatatagtg tttatgggta catattgcaa
     7621 tagttatttt tctgtgcatt tatatatgtg ctttatgtat aattattgga taatttcaat
     7681 tgtcttgaca aaaatatcta gttaccatag aataatatta acacgtgtta ataggcgata
     7741 attcattaat ataccgaaat actcactttt tgatgtaata ctcaaaataa aataattacg
     7801 aaaaaaagag tacatgacca tggacttttc aaagcagaat ttcttataga aaatatctta
     7861 aaaggatagc tgttctaaaa cctagaattg atttttactg gaattgttta tataggggag
     7921 gattgtatat gttggttaaa agaaggattt tatcagtgat tgtttttata attgtgtcga
     7981 ttttgattat aaagcttcca ttgtcggtga gtgcagcaca ggtaaaagca ggttcgggaa
     8041 gttacactga tgtattgcct tcaggatgta tttcaccgca atcaaccata tacaagactt
     8101 cgaatcttaa gggggcaatt cccacaagca gttgggaaag ctccattctt tggtatcagt
     8161 attcactccc catatttgcc catcccctga ccttttgctt taagccggaa ggattggaaa
     8221 taggtaatcc ggcattggga gggggaggaa tagcttactt tggtccccac aagaatgatt
     8281 tttcaatagg ccattcctca attggagtat ttcccgatgt ccaggcagat aaaatatcgg
     8341 attttgctgt tgatgcagta atgtattctg ggaatgcagg ctttaaatca acaattgtta
     8401 aaggaagccc ttatgcttac tttgcattca ccggcggcaa tcctgttatt tccttttttt
     8461 cggcaccggt gttattctac ggaaactggg atacgcagtg tttgggtgtt acaatcaacg
     8521 gtgttaacta tggattgttt gccccaacag gttccacatg gcagggaata ggaacgggga
     8581 cggtaacctg tattcttccg tcgggtaaaa actatttttc aattgccgtt ttacccgata
     8641 actccatcca gaccctttta tactataagg aacgtgcata cagctttgta acggatacaa
     8701 aggtgaactg ggagtatgat gaggagaata gccgggttac tacaaccttt agcgtagaaa
     8761 ccacgcctat ggagggtacg aataagaaca caatatttgc tttgtatccc catcaatgga
     8821 ggaataaccc tcatatatca actcttccgt atagttattc cactctaagg ggaattatga
     8881 agaccacaga gggtacatca tttaagaccg aatacaagta ccaaggtatt ttaccttcat
     8941 tgcccgataa aggaacttac gacaaatcaa ttttgaactc gtatattaat gaactgacag
     9001 cacaggcgaa ttctccccaa tcgcaagaca cct
//
LOCUS       DAOOJC010000035         8993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_6779_length_8993_cov_3.598009, whole genome shotgun sequence.
ACCESSION   DAOOJC010000035 DAOOJC010000000
VERSION     DAOOJC010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6779_length_8993_cov_3.598009"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..711)
                     /locus_tag="PLA01_01825"
     CDS             complement(67..711)
                     /locus_tag="PLA01_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HPD00071.1"
                     /translation="MAKLPVKLTFGEEVANSVSHGVASLAVLFAFPFACITAYNNGKI
                     ADVVGVTIYCVSIFLMFLMSTLYHAMEHETTHKSVLHILDHIFIYVAIAGTYTPVAIS
                     VIGGWQAVLILIIQWVMVLFGILYKSISRRSMPKVSLMIYLIMGWTLLIFMPLFVEKA
                     NPLLFWLILGGGIFYSIGAVIYARKGFKFFHMVWHIFVFLGSLTHFIGICFFLY"
     gene            928..2808
                     /locus_tag="PLA01_01830"
     CDS             928..2808
                     /locus_tag="PLA01_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HPD00072.1"
                     /translation="MGRLRKISAIAFVLVLIVSLLSGCQDADKDKNAEESPSITEIKE
                     KYNDSSLSVDEKVSYLIEIMTLEEKVGQMVQPDRSAASPKDVSRYFIGSVFSGGGNAP
                     QPNTMESWLEMCENYQESAMSTRLGIPIIYGVDAVHGHNTLPDAVIFPHNIGLGATGN
                     PELVAEVARVTAAEMIATGVSWNFAPCVAVARDQRWGRTYESFGENPELASKLLVPYI
                     KAMQDEYKIITTAKHYLADGGTKWGTGEGNHLIDQGDAQISEEELRKIHLPVYEEAVK
                     AGVKTVMASFSSWNGVKMHENQYLIQDVLKGELGFEGFVISDYEAVHQLSGKNLYSQL
                     VASVNAGVDMFMEPLHWMECITNIKKAVKNGDITEERINDAVSRILKVKFEMDLFNNP
                     LGNRELAADTLGNDENREIAKKAVRESLVLLKNERNVLPLKKKAKIFVSGPAADNVGI
                     QCGGWTKTWQGGQDNSGKWVKGSTILDGFKKIAEENGGTIYTDPDDINKADVAVIVIG
                     EKPYAEYEGDEEKLDLYSGKALEGNKEALELAKKARIPVVVILVSGRARIVTEEIDDW
                     NAFVAAWLPGSEGDAVAEVLYGDYEFKGKLPVTWPSSEANLKPGDRSCLFPCGFGFTM
                     TE"
     gene            2912..4183
                     /gene="dgt"
                     /locus_tag="PLA01_01835"
     CDS             2912..4183
                     /gene="dgt"
                     /locus_tag="PLA01_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HPD00073.1"
                     /translation="MDLDTHVQIEKECGYPRYRDQIEKIQSLYLAPFATKVPREGSRR
                     FEEKPYNYETRSEFQRDRDRIIHSKAFRRLLYKTQVFVNHEGDHYRTRMTHSLEVAQI
                     ARGIARSLGVNEDLVEAIALGHDLGHTPFGHAVESLLSGLLKEEQGFFHNEQSVRIVD
                     CIEVKKGLNPKLGLNLTWEVREGILKHNDDRTSGIYESLEPDKPSGIEGQIVALADTV
                     AYVCHDLEDGLEAGLFDDAIRCGLLNKQDIERLWDMFDAGLENGVSSIINRLVKDIVD
                     SAWNVLRDNNIDSVQKVRKFRDEGTNNRIVRLGKYKKSFKELKGFVGKYVYGNTMTAI
                     MDTKAKKFIGEIYESYLKNPEQLPGDVYKSFIDASKACDTVGGNTGERDDLNDGYRDT
                     PARVLCDYIASMTDRYALETYDRLFDPHIKI"
     gene            4330..4920
                     /locus_tag="PLA01_01840"
     CDS             4330..4920
                     /locus_tag="PLA01_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00074.1"
                     /translation="MNKLMGFLELKASDLPAVPWKEFNDKVRFDEKLLWTVRTAVEKG
                     EDLNLPRVVGVDAKEAYESAMRLYNKYKDIGMIVYYPYFVAEKSGTLYADNSKTVIEA
                     VGEDLWNFVTYNKKEVTVIISLDKGVCVEGNGEFLDKKEIQELCLYASRIRGMFRENI
                     AKGEGVLLEWSYAYNSDLNKQPVGDRYLVFYEIRTV"
     gene            4932..5117
                     /locus_tag="PLA01_01845"
     CDS             4932..5117
                     /locus_tag="PLA01_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00075.1"
                     /translation="MNHYSEEELKEAIRSIESTISKCEKVLPKLKQGSSQQTLLVRRI
                     KAFQIALSLMKEKLGDK"
     gene            complement(5296..5862)
                     /locus_tag="PLA01_01850"
     CDS             complement(5296..5862)
                     /locus_tag="PLA01_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00076.1"
                     /translation="MSDNNFYSNSTSTEQSDTPNNDIQPEQFGAQNDNAHSEQPQAAN
                     PFRRDWEPEAASNLGMGILGGLGAALIGAIIWAAITIFTGYQYSIAAVGMGLLVGGAV
                     RLLGKGWEMQYRITGAVLSMLGCLLGNIFSIYAVIAGRLNISFFQVLATTSILQTIRY
                     LQLTFSFYDILFYGVALVIGYKISAIDN"
     gene            6171..8399
                     /locus_tag="PLA01_01855"
     CDS             6171..8399
                     /locus_tag="PLA01_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019851501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HPD00077.1"
                     /translation="MGKILVLAEKPSVGRDLARVLNCKQNGGGYLMGPKYIVTWALGH
                     LVTLADPEAYDKRYKKWDLELLPMLPDRMELVVIRKTAKQFNIVKELMRRDDVDQLVI
                     ATDSGREGELVARWIIKKAGFKKPVKRLWISSQTDRAIRDGFNSLRPAGEYDNLFKSA
                     QCRAEADWLVGLNITRALTCKYNAQLSAGRVQTPTLAMVIERENEIKRFVPKDYWTIE
                     ADVKGFGSGISQNEIAQKRWGLNNPGALVLHWRDKKGQTRIFDRESADKIVNKVKGQN
                     GKVVEVIREAKSEPVPLAYDLTELQRDANRIFGYSAKKTLSIMQKLYESHKLVTYPRT
                     DSRYITEDIVPTLSERLKSIAVGPYAAAARELIKNKPCITRRFVDNSKVTDHHAIIPT
                     EQYVSLVSLDKDERNIYDLIVKRFIAVMSRPFEYEQTTIKVEAVGEQFYAKGKIVKSL
                     GWKAVYQGQPDSDDQDPEERDQVLPDIKKGADLCIGSVKAVNRKTKPPARYTEATLLS
                     AMENPGKFVGDRELREILDNVSGLGTPATRADIIEKLFDSFYMERNGKEIIPTSKGMQ
                     LINLVPEDLKSPELTAKWEQQLTGISRGKVSDSEFIFEMKKYASRLVSNVIANSSSFR
                     HDNITREKCPECGKYLLDVKGKKGKMLVCSDRECGYRKNVSQSSNARCPECHKKMELR
                     GEGEDKTFYCVCGYRERLESFKKRKSEQVDKRDVREFLKQQESTEPINTALADALKKL
                     KL"
     gene            8465..>8993
                     /locus_tag="PLA01_01860"
     CDS             8465..>8993
                     /locus_tag="PLA01_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00078.1"
                     /translation="MSAGRLKKTKRKYKFGGKESKTVFLLIIGILAIVGFFLSKGSIT
                     QFFKVFAVEAAVFALIVFCILTVYKNKKGSRSAYGSKKHKNFHEMNDVRKIEDDSKYV
                     NEKIKGGNINNVKTGEKNYNWVLDDESTQGSHRQKNNIETGRKLVYAGKIVSGEKKTM
                     GDEDIKARVASWAKAV"
BASE COUNT         2919 a         1483 c         2265 g         2326 t
ORIGIN      
        1 cgtacggttc aacgagaggg aaataattct tagcaattat ttctctactc tattctaagc
       61 tgcggtttag tacaaaaaga agcaaatgcc gatgaaatga gtcagggaac cgagaaaaac
      121 aaatatatgc cataccatat ggaaaaattt aaatcctttg cgggcataga tgactgctcc
      181 gatggagtaa aatatacctc cgccaagaat cagccagaac aaaagaggat tggctttttc
      241 gacaaaaagc ggcatgaaaa tcaggagggt ccagcccata atcaggtata tcataaggga
      301 tacttttggc atagaacgtc gggagatgga tttgtaaagt atgccaaaca gtaccataac
      361 ccactgtatt atgagtatca aaacagcctg ccagccaccg ataacggaaa tcgctaccgg
      421 tgtatatgta cctgcgatag ccacatatat aaatatgtgg tcgaggatat gcaatacaga
      481 cttgtgggtt gtttcatgct ccattgcgtg gtaaagggtt gacattaaaa acattaaaaa
      541 aattgaaaca caatagatgg tgacacctac aacatcagcg attttaccgt tgttatacgc
      601 cgtaatgcat gcaaagggaa aggcaaacag tactgccagc gaagcaactc cgtgacttac
      661 agagtttgca acctcttctc cgaatgtcag tttaaccggt agttttgcca aatatattac
      721 ctccttcaag gtcaaatgct cacttgctat tataccacca aaacacaaat gcattgtaaa
      781 ttatttatta aatttaattg tatggcaata acaaatccag cgaagggcaa gaatgcaaat
      841 aaaatcaatt ttcaatcata ttttcaaggt gattgttttg gtgtataatg aaataaattt
      901 attacttcat aaaaagggga gaaaacaatg ggtagattga gaaagatcag tgcaatagca
      961 tttgttttgg tattgatagt ttctttatta tcaggatgtc aagatgcaga taaggataag
     1021 aacgcagaag aaagcccatc aattacagaa ataaaagaga aatataatga ttcgagcctt
     1081 tctgtggacg aaaaagtaag ttatttgatt gagataatga ctcttgagga aaaggttggg
     1141 cagatggttc agccggacag aagtgccgct tcacccaaag atgtaagtag atattttata
     1201 ggatcggtgt tcagcggcgg tggtaatgcc ccgcaaccca acacaatgga aagctggctg
     1261 gagatgtgtg agaactatca ggaatcagca atgtctaccc gccttggaat tccgattatt
     1321 tatggagtgg atgcggtaca cggtcacaat actttgcccg atgccgttat atttccgcac
     1381 aatattggat tgggtgcgac aggaaatccc gagctggtag ctgaggtagc gagggttact
     1441 gctgccgaaa tgattgcaac cggtgtaagc tggaattttg ctccctgtgt agctgttgca
     1501 cgggatcaaa gatggggaag gacttatgaa agtttcggtg agaatcccga actggcaagc
     1561 aagctcttag ttccgtatat aaaggcaatg caggatgagt acaagattat aacaactgcc
     1621 aagcactatt tagctgacgg aggtaccaaa tggggaaccg gagaaggtaa ccatttgatt
     1681 gaccagggag atgcacagat atcggaggag gaactcagaa agattcacct tcctgtgtat
     1741 gaggaggctg ttaaggccgg ggtgaaaacg gttatggctt ccttcagcag ttggaacgga
     1801 gtgaaaatgc acgaaaacca ataccttatt caggatgtct taaaaggcga actgggcttt
     1861 gagggctttg ttatatcgga ttatgaggcg gtacaccagc ttagcggcaa aaacttgtac
     1921 agccaattgg tggcttcggt gaatgccgga gtggatatgt ttatggagcc tttgcactgg
     1981 atggaatgta tcacgaatat aaagaaggca gtgaagaacg gtgacattac agaggaaaga
     2041 ataaatgatg cggtaagcag aattttgaag gttaagttcg aaatggattt gtttaacaat
     2101 ccgctgggga atcgtgaatt ggctgcagac accctaggca atgacgaaaa ccgtgagatt
     2161 gcaaaaaagg ctgtaagaga atcactggtg cttctcaaaa atgaaagaaa tgttctgccc
     2221 cttaaaaaga aagcaaaaat atttgtttcc ggtcctgcag cagacaacgt aggtatacag
     2281 tgcggaggct ggaccaaaac atggcagggc ggacaggata actcaggtaa atgggtaaaa
     2341 ggctcaacta ttcttgacgg ttttaaaaag attgccgaag agaacggagg aacaatttac
     2401 accgaccctg acgatattaa taaggcagat gttgcggtaa ttgttatagg tgagaagcct
     2461 tatgcagagt acgaaggcga tgaggaaaag cttgatttat attccggcaa ggctctcgag
     2521 ggcaataagg aagcattgga gcttgccaag aaagcccgta ttcctgtggt ggtaatactg
     2581 gtttcgggaa gggccagaat agttaccgag gagattgatg attggaatgc tttcgttgct
     2641 gcatggctgc cgggaagcga aggagatgca gttgcagagg tattatacgg tgactacgaa
     2701 tttaaaggca agctgcccgt tacatggcca tcgagtgaag ccaacctaaa acctggtgat
     2761 agaagctgtt tattcccctg cggttttggg tttacgatga ctgagtgatc caaaacatga
     2821 gttgaaataa caaaaagctc aagggctcga agctcttgag cttttgacag tgaagagagc
     2881 aggtaagatt gttaatttag agggaggagg aatggatttg gatacacatg tccagataga
     2941 gaaggaatgc ggttatccac ggtataggga ccagattgag aagattcaaa gcctgtacct
     3001 tgcccccttt gcaacaaagg ttccgaggga ggggagcaga aggtttgaag agaagcctta
     3061 taattatgaa acaagaagcg agtttcagag ggatcgggac aggattattc attcaaaggc
     3121 attcaggaga cttttatata aaacccaggt atttgttaac catgaggggg atcattatag
     3181 gaccagaatg acccacagtc tggaggtggc tcaaattgcc cggggtattg cccgttctct
     3241 tggagttaat gaagacctgg tggaagcgat tgccctgggg catgacttgg gacacacacc
     3301 tttcggacat gctgtcgaga gcctgctttc cggtttgctg aaggaagaac agggcttttt
     3361 ccacaatgag caaagtgtga ggattgttga ttgtattgaa gtaaaaaaag gtctgaatcc
     3421 caagcttggt ctcaatctta cttgggaggt aagagaagga atcttaaagc ataatgatga
     3481 taggacttct ggaatatatg agtctttgga accggacaag ccgtcgggta tagaaggaca
     3541 aatagtggca ttggctgata cggtggctta tgtatgccat gacctggagg atgggcttga
     3601 agcaggattg tttgatgatg cgattcgatg cggattattg aataaacaag atatcgaaag
     3661 gctctgggac atgtttgatg cagggcttga aaacggggta agcagtatta taaacagatt
     3721 ggtaaaggat attgttgatt ctgcatggaa tgttttgcgg gataataata tagattcggt
     3781 ccaaaaggta aggaagttta gggatgaagg aacaaacaac agaatagtca ggctgggaaa
     3841 atacaaaaaa agttttaaag aacttaaggg ctttgtgggt aagtatgttt acggaaatac
     3901 gatgactgcc attatggata ccaaagcaaa gaaatttatt ggggagatat atgagtcata
     3961 cttaaaaaat cccgaacagc tgccgggtga tgtatacaaa agctttattg atgcttcgaa
     4021 ggcttgtgat actgtaggcg ggaacacagg ggaaagggat gatttaaacg acggatacag
     4081 ggatacaccg gccagggtgc tctgtgatta tatagcatcc atgactgaca ggtatgccct
     4141 cgaaacgtat gacagattgt ttgaccctca tattaagata tagtcaatgg ttttgtccga
     4201 ttggtcagaa ggaggctgaa cttcaatatt caaagacggt tataggaaac ggttttctta
     4261 ttgccggtct ttaggtataa gtaccatccg atgcccttga taaaatcgga tgattgggag
     4321 gatttcttaa tgaataagct aatgggtttt ttagagttga aggcctcaga ccttcctgct
     4381 gttccgtgga aagagttcaa tgataaggtt cgctttgatg aaaagctttt gtggacggtc
     4441 aggacggcgg tggaaaaagg tgaagacttg aatttgccaa gggtcgtggg agttgatgcc
     4501 aaagaggctt atgagagtgc tatgaggctt tataacaaat acaaggatat cggtatgatt
     4561 gtatattatc cttattttgt tgcagaaaaa agcggaaccc tttatgcaga taattccaag
     4621 acagtgatag aggcggtagg agaggattta tggaactttg ttacttacaa taaaaaagag
     4681 gtaacggtta ttatcagttt ggataagggt gtttgtgttg aaggcaatgg cgaattcctt
     4741 gataaaaagg aaattcaaga gctttgttta tatgcctcaa ggataagagg tatgttccgg
     4801 gagaacatag ccaaaggcga aggagtactt ctagagtgga gctatgccta taattccgat
     4861 ttgaacaaac agcctgtagg agacagatat ctggtttttt acgaaataag aacagtgtaa
     4921 atggggttaa tatgaatcat tacagtgaag aagagttaaa agaagctata cgatctatag
     4981 aatcaacaat cagcaagtgt gaaaaggtac tgccaaagtt gaaacaaggc agttctcagc
     5041 aaacacttct tgtacgcaga ataaaagcgt tccaaattgc attgtcattg atgaaagaga
     5101 aattgggaga taaataatat aaaataaaga gaattggatc acggagcaat acattgtgtg
     5161 gatattcaga ttcgggaacg taacattatt tcaatttatg taaattctgc atgtacctat
     5221 agaaaaagct ggggagctaa accccccaag ctttatcgaa ttttcaaagt attaaaactt
     5281 gttatgtaat aatccttagt tgtctatggc agagattttg tatcctatta ctaatgccac
     5341 accatagaat aaaatatcat agaaactgaa tgtcagttga agatatctga ttgtttgaag
     5401 gatactggtg gtagcaagta cttgaaagaa ggatatattt aaccttccgg caataactgc
     5461 atagattgaa aatatgttgc ccaaaaggca tccaagcatg gacagaacag ctccggtgat
     5521 gcgatattgc atttcccatc cttttcccaa tagtcttacg gcaccaccta caagcaatcc
     5581 cattccaaca gcagcgatag agtattgata acctgtaaat attgttatag cagcccatat
     5641 aatagctcca atcaatgccg cacccaatcc gcccagaatg cccataccga gatttgaggc
     5701 tgcttccggt tcccagtcgc ggcgaaaagg attggcggct tgaggttgct ctgaatgtgc
     5761 attatcgttc tgcgcaccga attgttctgg ttgtatatcg ttgttcgggg tatcggattg
     5821 ctccgttgaa gtgctgttgg agtaaaaatt gttatcggac atgatttacc tccttgaaat
     5881 atatatatac ttattttaac attacataaa atgtagagca attcatttta ttaaaatatt
     5941 ccttttatcc ttaaattatg ttaatgggag aagcagagag attgttttca gcaaggccgg
     6001 atttttccaa aacctatgga agtgtgcata tggtttttca catatatttc atgcacccat
     6061 atagaagaaa ttgtgctgaa gtgagtacga ttttgtgata tagtagatac cggaaaactt
     6121 attgattaag ttcaaaatac attctaaatg taaaaggaaa ggaattcatg atgggtaaga
     6181 ttttggttct ggcagaaaag ccatcggtgg gacgagatct cgcaagggtt ttaaactgca
     6241 agcagaatgg cggcgggtac cttatggggc ctaaatatat tgtaacatgg gcattgggtc
     6301 atcttgtgac tttagcggat cccgaagctt acgataaaag gtataagaaa tgggaccttg
     6361 agttgcttcc gatgctgccc gacagaatgg aactggtggt gatacgaaag actgccaaac
     6421 agtttaatat agtaaaagag ctgatgagaa gggatgatgt agaccaactg gttatagcta
     6481 cggactccgg ccgcgaaggg gaactggtag cacgatggat tattaagaaa gcaggtttta
     6541 aaaaacctgt gaagcgtttg tggatttctt cccaaacaga cagggcaata agggacgggt
     6601 ttaatagttt aagacctgcc ggagagtacg ataatctatt caaatcagcc caatgccgtg
     6661 ctgaagccga ttggttggtg ggactcaata taacaagagc acttacatgc aaatacaatg
     6721 cccagctttc ggcagggagg gtgcaaacac ctactcttgc catggttata gaacgggaaa
     6781 atgaaataaa gaggtttgtc cccaaagatt attggacaat tgaagctgat gtaaagggtt
     6841 ttggaagtgg gatttcacaa aatgaaatag cccaaaagcg atgggggctc aataatcccg
     6901 gagctttagt tcttcattgg agggataaga agggccagac aaggattttt gacagggaaa
     6961 gtgccgataa aatagttaat aaggtcaaag gccagaatgg aaaagttgtg gaggtaataa
     7021 gagaggcgaa aagcgagcct gtacctttag cttatgacct tactgagctg cagagggatg
     7081 caaaccgcat attcggctat tccgcgaaaa aaactctttc gattatgcag aagctgtatg
     7141 agagccacaa gctggtgaca tatccgagaa ccgattcaag atatataacc gaagatattg
     7201 ttcccacgct ttcggaaaga ctaaaaagta ttgctgttgg gccttatgcc gctgctgcca
     7261 gggaattgat aaaaaacaag ccttgtataa cccgaagatt tgtcgacaac agcaaggtta
     7321 cggaccacca tgccattata ccgacagagc agtacgtaag cctcgtgtcg ctggataaag
     7381 atgaacgcaa tatttacgat ttgatagtta aaaggtttat tgccgttatg agcagaccct
     7441 ttgaatatga acaaaccacg ataaaggtag aagctgttgg agaacagttt tatgccaagg
     7501 ggaaaatagt aaagtcattg ggctggaagg ctgtttatca gggacagccg gactccgatg
     7561 atcaagatcc tgaggagaga gatcaggttc ttccggacat aaagaaagga gcagacttat
     7621 gtatcggttc tgtaaaggct gtgaaccgta aaacgaaacc tcccgcaagg tatactgagg
     7681 caacactgtt gtctgccatg gagaaccccg gtaagtttgt gggagacaga gaactaaggg
     7741 agatattgga taatgtcagt gggttgggga caccggccac acgtgccgat ataattgaaa
     7801 agctctttga ttccttttac atggagagga atggaaagga gattatcccc acatcaaaag
     7861 gtatgcagct gataaatctt gtgccggagg acttgaagtc tccggaatta accgcaaaat
     7921 gggagcaaca gcttacagga atcagcagag gaaaggtcag tgattcggaa tttatctttg
     7981 aaatgaagaa atatgcatcc cgcttggtat ctaatgttat cgccaattca tcgtctttca
     8041 ggcacgacaa tatcacacgg gaaaaatgtc cggagtgcgg caagtatctg ttggatgtaa
     8101 aaggaaagaa agggaaaatg ctggtttgtt cagaccggga atgcgggtac aggaaaaatg
     8161 tttcccagtc atccaatgca agatgtcccg aatgtcataa gaaaatggag ctacgcggtg
     8221 aaggtgagga taaaaccttt tattgcgtat gcggataccg tgaaaggctt gagtcattca
     8281 aaaagcgtaa aagcgagcag gtggacaaga gagatgtaag agaattttta aagcagcaag
     8341 agagcactga accgattaac acagctttgg cagatgcatt gaaaaagctt aagctgtaaa
     8401 tagcagacaa taaagtatca gtttattaaa gtttcattta taatgagaag gtgggtatga
     8461 atttatgagt gccggaaggc ttaagaagac caaacgtaaa tataaatttg ggggcaagga
     8521 atcaaaaact gtttttcttt tgattatcgg aattttggcg atagttggtt ttttcttgtc
     8581 taaaggttca ataacccaat ttttcaaggt gtttgcggtt gaagctgctg ttttcgcctt
     8641 gattgtattt tgcatattga ctgtatataa aaacaaaaaa ggttcaaggt ctgcttacgg
     8701 aagcaaaaag cataagaatt ttcatgaaat gaatgatgta agaaaaatag aggatgacag
     8761 taaatatgtt aacgaaaaaa ttaaaggtgg gaatataaat aatgtcaaaa ccggggaaaa
     8821 gaattataat tgggttttag atgatgaaag tacgcaaggt tcccacagac agaagaacaa
     8881 tatcgaaacc ggcaggaagc tggtatatgc cggtaagatt gtttccggtg aaaagaagac
     8941 aatgggtgac gaggatataa aagccagggt ggccagttgg gctaaagccg tag
//
LOCUS       DAOOJC010000036         8950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_6821_length_8950_cov_3.492074, whole genome shotgun sequence.
ACCESSION   DAOOJC010000036 DAOOJC010000000
VERSION     DAOOJC010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6821_length_8950_cov_3.492074"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="PLA01_01865"
     CDS             complement(<1..1224)
                     /locus_tag="PLA01_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPD00079.1"
                     /translation="MKLLEKIFGSYSDRELKRIMPIVDKIEAFEPEIQALSDEQLKAK
                     TPEFKKRLSEGETLDDILPEAFAVVREASKRVLGMRHFRVQLIGGVVLHQGRIAEMKT
                     GEGKTLVATLPVYLNALEGKGVHVVTVNDYLARRDSEWMGKVYNFLGLSVGLIVHGLN
                     NSERRAAYNCDITYGTNNEYGFDYLRDNMVIFKQDMVQRDLHFAIVDEVDSILVDEAR
                     TPLIISGVGDKSTDLYTRVDAFVRRLKVKVFTETDDKQYNDDVEADYIVDEKANTATL
                     TADGVKKAEEYFGVENLSDPDNLTLSHHINQALRAHGIMRKDKDYVIKDGEIIIVDEF
                     TGRLMYGRRYSNGLHQAIEAKEGVKVERESKTLATITFQNYFRMYKKLSGMTGTAQTE
                     EEEFQSIYRLDVIVIP"
     gene            complement(1252..1839)
                     /locus_tag="PLA01_01870"
     CDS             complement(1252..1839)
                     /locus_tag="PLA01_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HPD00080.1"
                     /translation="MLYFVVIIAAAVLAVVYGIIKKRSFDLFNIKLQKTWLIVVAFAL
                     QTLTRLMGVNGIDFVVKYSLIIQTAVFALLFLCFWFNKKYIGLWFIGLGASLNALVMT
                     VNGGRMPVGLEAMQKAGIQAVTDAILSGSDNKHIVLSETTKLGFLADIIYLPGVLGWG
                     IGVLSIGDAIVALGIFIFVFQLCTSSMKLSDVGHE"
     gene            complement(1918..2325)
                     /locus_tag="PLA01_01875"
     CDS             complement(1918..2325)
                     /locus_tag="PLA01_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00081.1"
                     /translation="MFKKLFLSLLILSALTTEGSLNISEYIITPLLFENLGLNNFTDC
                     EVLPVNERVLPERKGTEGPVLISDANFSKYPTLPEEDNRGGIRPAVTDSYNCCTDLSV
                     SYFRRLEAEGADSFINKDISLHMSDLSPPCKII"
     gene            complement(2432..2728)
                     /locus_tag="PLA01_01880"
     CDS             complement(2432..2728)
                     /locus_tag="PLA01_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HPD00082.1"
                     /translation="MNSKIKDEMTDRLFEAILLLENVEECYNFFEDICTISEIKALAQ
                     RLEVAKMLRDKKTYTEITEKTGASTATISRINRALNYGADGYKTILDRLYSKNK"
     gene            3568..4887
                     /locus_tag="PLA01_01885"
     CDS             3568..4887
                     /locus_tag="PLA01_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HPD00083.1"
                     /translation="MNKKTVFDIPAQTNHIIETLNKNGFDAYIVGGCIRDIILGQTPQ
                     DWDITTDAKPDDVKRLFPNVFETGIKHGTVTAVIDGTNYEITTFRASNSITPSTLTED
                     LGLRDFTMNAIAYHPNVGIIDPFSGLSDIEESIIRAVGNPDNRFEEDPLRMLRAVRFS
                     STLEFKIDVPVLSSIKENSGKIERVSPERIRSELSKILTSDRPEGFVILRETGLLKHI
                     LPEFDICFDITQNHPYHIYNVAIHSLHTVASIEKNTVLRWTMLLHDTGKAVTKTTDNK
                     GIDHFYGHPQKSMEIAKRVLGRLKFDNASVERILSLIKHHDMQINPDYKSVRKAASKM
                     GEDIFLDLLKVQEADKSGQNPKYSDERSDTLNKIREIYYDIKKNNHCLTIKDLAINGD
                     DLLAMGFEQGHDVGVVLKMLLNAVLENPELNTREKLLEMVDDVGNSF"
     gene            complement(5122..5658)
                     /locus_tag="PLA01_01890"
     CDS             complement(5122..5658)
                     /locus_tag="PLA01_01890"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008375098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HPD00084.1"
                     /translation="MSLTAISKYMSLILRHKPETIGISLDEHGWADVDELIMGIAKTH
                     EFNMDILEEIVYSDNKQRYSFNEDKTLIRANQGHSIPVDVELDEVEPPIELWHGTGAK
                     YVASIDAKGLIPKSRLYVHLSEDIDTAVKVGMRHGKPVLYIVKAGDMFSDGYKFYLSK
                     NGVWLTKQVPVKYLVKQE"
     gene            complement(5837..5920)
                     /locus_tag="PLA01_01895"
     tRNA            complement(5837..5920)
                     /locus_tag="PLA01_01895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5884..5886),aa:Leu,seq:tag)
     gene            6417..7220
                     /locus_tag="PLA01_01900"
     CDS             6417..7220
                     /locus_tag="PLA01_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPD00085.1"
                     /translation="MIILYIIIAIAVLALVYMFFETTQLKVNRVYYRECSNPLKVIHL
                     SDLHLIYLNVNINKITKVLKEESPDLVIITGDYINSVSHIPLFLKFLDDIKGNYRILI
                     CLGNHDYKTLNNNEQEIRKFIKGIEEKGATVLMNDSICFEKDNRKYNIIGIEDLRSNR
                     YDVKKALESCSSDAVFNIAFSHNPDIIFQIPEGTVDYLLCGHFHGGQIWMPFNLEFII
                     LRNEKLCKMGITKGAHRVNNINLYINSGLGNVCFPLRFLSPPELTVLHF"
     gene            complement(7340..8686)
                     /locus_tag="PLA01_01905"
     CDS             complement(7340..8686)
                     /locus_tag="PLA01_01905"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPD00086.1"
                     /translation="MKSLKFDYSKATGFVKENEVAYFESFIKSAHEMLHNKSGAGNDF
                     VGWVDLPIDYDREEFSRIKAAAEKIKSDSQALVVIGIGGSYLGARAAIDVLSHSFHNV
                     LPESKRKAPQIFFVGNNISSTYIADLLEAIEGKDISVNVISKSGTTTEPAIAFRIFKE
                     YMENKYGKDGARSRIYATTDKDKGALKKLATEEGYETFVVPDDIGGRFSVLTAVGLLP
                     IAVAGIDIDSMMKGAQDARELYSNPNLAENDCYKYAAVRNALYRKNKTIEIMVNYEPS
                     LHYFTEWWKQLYGESEGKEQKGIFPAGVDFTTDLHSMGQYIQDGLRNIFETVIKVEKP
                     SKNIIIKEDKDNLDGLNFVAGKDIDYVNKKAMEGTLLAHTDGGVPNLVVSVPELNAYY
                     FGSMVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEEQRKKLEERL"
BASE COUNT         2758 a         1900 c         1455 g         2837 t
ORIGIN      
        1 cggtattaca ataacatcca gcctgtagat gctctggaat tcctcctctt cagtctgagc
       61 cgtacccgtc ataccggaaa gctttttata cattctaaag tagttttgga atgtaatggt
      121 cgcaagggtc ttgctttccc tttcaacctt tactccttcc tttgcttcaa tcgcttgatg
      181 cagtccgttg ctataccttc ttccatacat aagtcgtccg gtaaactcat caactataat
      241 tatttcgccg tcttttatta catagtcctt gtcttttctc ataataccgt gagcccgcaa
      301 agcctggttt atatgatgcg aaagggtcag attatccgga tcggaaaggt tctcaacccc
      361 aaaatactct tctgcctttt ttacaccgtc agcagtcaag gttgccgtat tggccttttc
      421 atccactata taatcagctt caacgtcgtc attatattgc ttgtcatccg tttctgtaaa
      481 cacttttact ttcagtctcc tgacaaaggc atcaaccctt gtgtaaaggt cagtggattt
      541 gtctcccacc ccggatataa taagaggagt tctggcctca tccacaagaa tactgtccac
      601 ctcgtccaca atggcaaaat ggagatccct ttgaaccata tcctgcttga aaataaccat
      661 attatccctc aggtaatcaa acccatactc gttattggtt ccataggtta tatcacagtt
      721 ataggcagct cttctctcac tattgttaag tccatgtaca ataaggccaa cactgagtcc
      781 taaaaaattg tatacctttc ccatccactc actgtcacgc cttgccagat aatcgtttac
      841 ggtaacaaca tgcacccctt taccttccag ggcgttcaag tatacaggca gtgttgcaac
      901 caatgtcttt ccttcaccgg ttttcatttc agctattctg ccctggtgaa gcacgacacc
      961 tccaatgagc tgaaccctga agtgcctcat gccaagaacc ctttttgaag cttcccttac
     1021 caccgcaaaa gcttcgggca aaatgtcatc tagagtctcc ccctctgaaa gcctcttttt
     1081 aaactcggga gtcttagcct tcagttgttc atcgctcaat gcctgtattt ccggctcaaa
     1141 agcctcaatt ttatcgacaa tgggcattat ccgcttaagt tctctatcac tataacttcc
     1201 aaatattttt tccaataatt tcatctttca caaacctcca gatataatat attattcatg
     1261 tccaacatcc gataatttca ttgaagaagt acaaagctga aatacaaata taaaaattcc
     1321 caatgcaaca attgcatctc caatgcttaa tactccgata ccccaaccca atactcctgg
     1381 caagtatatg atatcggcta aaaaccccag ctttgtggtc tcgcttaaga ctatatgctt
     1441 attgtccgaa cccgataata tcgcatcggt taccgcctga atacctgcct tttgcattgc
     1501 ctcaagacca accggcattc tgcctccatt cactgtcata accaatgcat tcaatgatgc
     1561 accaagtcca ataaaccaaa gtccaatata tttcttgtta aaccaaaagc acaaaaacaa
     1621 taatgcaaat accgctgttt gaattattaa gctgtatttc accacaaagt caatgccgtt
     1681 aacacccatt aatcttgtca gcgtttgaag ggcaaaggca acaactatca gccaagtctt
     1741 ttgaagtttt atattaaata aatcaaagga ccttttcttt attattccat acacaactgc
     1801 gagtacggca gctgcaatta taacgacaaa ataaagcaag ttatcacctt acctttgtta
     1861 gcgtcatttt ccgcatacct tattcactga cagtttcaaa cattattgac ctgtacccta
     1921 gattatttta cagggaggtg ataaatcgga catatgaagg cttatatcct tatttataaa
     1981 agaatctgca ccttctgctt ctaatctacg gaaataagaa acagacaaat cggtacagca
     2041 attataactg tctgttaccg caggacgtat tccgcctcta ttatcttctt ccggcaaagt
     2101 aggatatttc gaaaaattag catcggaaat aaggacagga ccttctgtac ctttgcgttc
     2161 cggtaatacc cgttcgttaa ccggaagtac ttcacaatcc gtaaagttat tcagccccaa
     2221 gttttcgaaa agcaaagggg taattatata ttccgatata ttcagcgagc cttccgttgt
     2281 taaggccgat aaaatcagca aacttaaaaa tagcttctta aacattggta ccttccttaa
     2341 gattattcaa attatgctta tccttagtgt aataatatcc gattttttga atttaatcaa
     2401 cactaaaaaa actatgtata aaaatatctt ttcatttatt tttactgtat aacctgtcca
     2461 gaatagtttt gtaaccgtcg gctccataat taagtgccct gtttatcctg cttattgtag
     2521 ctgtgctggc cccggtcttt tcagttatct cagtataagt ctttttatct cttagcattt
     2581 tagcaacttc aagtctttgt gcaagagcct ttatttcaga aatagtacat atatcctcaa
     2641 agaaattata acactcctcg acattttcaa ggagcaatat agcctcaaac agcctatccg
     2701 tcatctcatc ctttatttta gaattcatac ttcctcaact cccaccattt taagacaaaa
     2761 aaccaatcct tctcaaataa caatatcagc tttttattaa acggtcaaga gtccatacac
     2821 cacctcaaaa tcagcaattt gcttactgca tttaatataa atttaatatt tctgtaatat
     2881 aaatgaaaaa aattatagaa tttagtcttt acatatggta taataaatat gagatttgta
     2941 atactcttaa atcctcatag taataaatga ggtttttttt tgcagagtcc atacaccgaa
     3001 ttcattttct cgtcatggta ttttctttat tcgattttcg gcactaacgt tctgaatttt
     3061 atccataata tggccttaca gtacccatta aataaactaa ctgtgacttc tagtgattta
     3121 cataactcaa tgtgatgctt tttgtagaat attttgtcga atttcgcgat attatgacgt
     3181 tatgttccat cattaaataa tatgttgtat attaattata gtaattttaa aaaagttact
     3241 cccctaaatc ccgttgcatt ctcccttctc tcattggaat gcacggggtc tttttttatc
     3301 tgtttgcaga taaccttttt ccgctgactc ttattgatat gtatactgtt gatgtatatt
     3361 attaatgtat attgttgatg tatgttattt acgtaaagaa aaatgcggaa gaaacattgt
     3421 gaagcaagtt gcagccgcct gttaatactc tattttaatg ttttataaaa tacaatttta
     3481 tgatacaaaa tattgatgtg gacgaattaa ttgggcaaat acccgtaaag ttttttatca
     3541 cttttatagt ttaaggggat ttattgcatg aataaaaaaa ctgtatttga tattcctgca
     3601 caaacaaacc atataattga aacgctaaat aaaaacggct ttgatgctta catagtgggc
     3661 ggctgtattc gcgacattat tctgggccaa actccgcagg actgggatat aaccaccgat
     3721 gcaaaacctg acgatgtaaa gcgtttgttt cccaatgtgt ttgaaaccgg tataaagcac
     3781 ggtacagtca ctgctgttat agacggtacc aattatgaaa taactacctt cagggcatcc
     3841 aactccataa cgccgtctac cttgacggaa gatctgggtt tgagggattt taccatgaat
     3901 gccatagcct atcaccccaa cgtgggaata attgaccctt tttcagggct ctcggacata
     3961 gaagaatcga taatccgtgc ggtcggaaat cctgataacc ggttcgaaga agacccgtta
     4021 agaatgctca gagcagtacg tttcagctca acgttggaat ttaaaattga tgtccctgtc
     4081 ctttcatcaa ttaaagagaa ctccggaaag atcgaaagag tcagtccgga aagaatacgc
     4141 agcgagcttt caaagatatt gacctccgac agaccggaag gatttgttat actgagggaa
     4201 accggtcttt taaaacatat tcttccggag tttgatatat gctttgacat tacccagaac
     4261 catccgtatc atatttacaa tgtagcaata cattctttgc atacagtggc aagcattgaa
     4321 aaaaacacag tactcaggtg gaccatgctt ttgcatgaca caggcaaagc tgttacaaaa
     4381 accaccgaca ataagggaat tgaccatttt tacggtcatc cccaaaaaag catggagata
     4441 gccaagaggg ttctgggaag actgaaattt gacaatgcat ccgttgagag aatccttagc
     4501 cttattaaac accacgacat gcaaataaat ccggattata aatcggtgcg gaaagctgcc
     4561 agcaaaatgg gtgaggatat ttttttggac cttctgaagg ttcaggaggc ggataaaagc
     4621 ggacaaaacc cgaagtattc tgatgaaaga tccgatacgc tgaataaaat acgggaaatt
     4681 tactatgaca taaagaaaaa caaccattgc ctgacaataa aggatcttgc cataaatgga
     4741 gatgaccttc tggctatggg gtttgaacaa ggccatgatg taggtgttgt tttaaaaatg
     4801 cttctcaatg ctgttcttga aaacccggaa ctgaatacaa gagaaaaact tttggaaatg
     4861 gtggatgacg tcggaaattc attttaagtg ttataaaaaa acaaatattt taggatagtc
     4921 tcggattact ggacatttca gatggccatt ttgcggataa tcttaataac agtattggat
     4981 aataaaataa gtaatgttaa ataaattgaa tatatttata aaataatcgg tatgatgtgc
     5041 ctttacaaat attacttcaa accgctatag tcgtattgag gaagagaaat tctatctcat
     5101 taatttgaaa tattaagttt tctactcctg ctttacaaga tatttcacag gaacctgttt
     5161 tgtaagccat accccattct tcgagagata gaacttatat ccatcactaa acatatcacc
     5221 ggcttttaca atataaagaa ccggttttcc atgacgcata cctaccttaa cagctgtatc
     5281 aatatcctct gacagatgca catacaaccg gctcttagga ataagtccct ttgcatcaat
     5341 tgatgccaca tattttgctc cggtaccatg ccaaagttct atcggtggtt ccacctcatc
     5401 caactcgaca tctactggaa tagaatgccc ctgatttgct ctaatcagtg ttttatcttc
     5461 attaaacgaa tatctctgct tattatctga gtacacgatt tcttccaaaa tatccatatt
     5521 gaattcatga gtctttgcga tccccataat aagctcatca acatcggccc aaccatgttc
     5581 atcaaggctg attccaattg tctccggttt atgacgcaga attaagctca tgtatttgct
     5641 tatagctgtt aaactcaaaa ataacacatc ctttacaaat gcatattcaa caccagtttt
     5701 tgctccatgc catttgaata tacatacata atccttgttc tatcaaacac aggatttgca
     5761 tcatgaataa tacaatgatg atttgggttg aaaaaaggtc tgagaaaact tataaaaaaa
     5821 tcctctcaaa ccttattggt gcggacgaag ggacttgaac ccccacgtcg gtgacactag
     5881 atcctaagtc tagcgcgtct gccaattccg ccacgtccgc atgcattagt cagcaacaaa
     5941 aattattata cttgcgtccg cggcatttgt caacagtaaa aaatctcatc ttcaaaattc
     6001 tgatagaaat acgatccatg atggaattat actgggataa tcccccattt tataagtaaa
     6061 ttccatcact gctttgtcaa tcaataaaaa acaggaatac tactaaagct acaattacta
     6121 gtgagacaag ataaacaaaa aactttagaa ttttcttccc aatcgatgat tctcttcgcc
     6181 gatagctttt gatagaatac gttctcggca caagttcata gtcattgtaa gaattgaaca
     6241 gcatcgttta gcacccctga taaaattata atctctattg aggtttaaaa tccagaggat
     6301 aatattgcta taggattata aaaatatatt gatgtaatcc gtttttcctg atatattatt
     6361 tgatagagat atttatttat ttggggagta aaattaagtt actaaggaaa tatatgatga
     6421 ttatactata tattattata gcaatagcag ttttagctct tgtttatatg ttttttgaaa
     6481 caacacagct aaaagttaat agagtctatt acagagaatg cagtaatccc ttaaaggtta
     6541 tacatctatc agaccttcat ttgatatact taaatgtgaa cataaacaaa ataacaaaag
     6601 tgttaaaaga ggaaagtcct gaccttgtca taattacagg tgactatata aacagtgtct
     6661 cccacatacc tttattttta aagtttcttg atgatataaa agggaattac agaatactta
     6721 tatgcctcgg caatcatgat tataagaccc ttaataacaa cgaacaggaa atacggaaat
     6781 ttataaaggg aatagaggaa aaaggtgcca ctgttcttat gaacgattca atttgttttg
     6841 aaaaggacaa ccgcaaatat aacataatcg ggattgagga cttaaggtca aaccgatacg
     6901 atgttaaaaa agctcttgag tcttgcagca gcgatgcagt tttcaatatt gccttttctc
     6961 acaaccccga tataatattt caaataccgg aaggaactgt ggattatctt ttatgcggcc
     7021 attttcatgg gggacagata tggatgccct tcaatcttga atttataatc ttaagaaacg
     7081 aaaaactatg taaaatgggc attacaaagg gagcacacag ggtaaacaat attaatttat
     7141 acattaacag cggtcttgga aatgtctgct ttcccttaag gtttttgtct ccgccggagc
     7201 tgaccgtact gcacttttaa tgtcgaaaag cccggggttc ccgagctttt ttatcatcat
     7261 gttttccgat tttatgccgc ataccttacc aataattaaa atccttactg taaagatcat
     7321 ttgatcttgt ctttatattt tacagacgct cttcaagctt ctttctctgt tcttcataac
     7381 ccggttttcc caaaagagca aacatatttt tcttatatgc ctcaacaccc ggttggtcaa
     7441 aggggtttac tcccaatagg tatccgctta tgccgcatgc cttttcaaag aagtatacca
     7501 tgcttccaaa gtaataagca ttaagctccg gtacagacac cacaagattg ggaacgccac
     7561 catcggtatg ggcaagcaag gttccttcca tagccttctt gtttacataa tcaatatcct
     7621 taccggcaac aaagttcaat ccgtcaaggt tatccttgtc ttcctttata ataatattct
     7681 ttgagggttt ttcaaccttt attaccgtct cgaatatatt tctgagccca tcctggatat
     7741 actgacccat agaatgaaga tccgttgtaa agtccacacc tgccgggaat ataccctttt
     7801 gttcttttcc ctcactttct ccatagagct gcttccacca ctcggtaaaa tagtgtagtg
     7861 aaggctcata gttaaccatt atttcaatag tcttgttttt cctgtatagg gcattcctca
     7921 ctgccgcata cttgtaacaa tcgttttccg ccagattggg gttactgtaa agctcccttg
     7981 catcctgagc ccctttcatc atgctgtcga tatcaatgcc ggctactgca atcggaagca
     8041 atcctacggc agtcagaacg gaaaatctcc cgccaatatc atcgggaact acgaaggttt
     8101 catagccttc ttcggtagcc agttttttaa gtgcaccctt atctttgtcg gtagttgcat
     8161 aaatcctgct tcttgccccg tctttcccat atttgttttc catatactct ttaaagattc
     8221 tgaatgcaat agcaggctcc gttgtagtac cggactttga tataacattt actgaaatat
     8281 cttttccttc aattgcctca agcaagtcgg caatgtatgt agaactgata ttgttgccga
     8341 cgaaaaatat ctggggagcc tttctctttg actccggcaa aacattgtgg aatgaatggg
     8401 acaacacgtc tattgcagcc ctggcaccta aataggaacc gccgattcca attacaacca
     8461 gtgcctggga atccgattta atcttctcag ccgcagcttt aattcttgaa aactcttccc
     8521 tgtcatagtc tataggaaga tccacccatc ctacaaagtc gttccccgct cctgacttat
     8581 tgtgaagcat ttcatgagcg gattttatga agctttcgaa atatgccact tcattttcct
     8641 ttacaaaacc tgttgctttt gaatagtcaa attttaagct tttcataact aacctccaat
     8701 ttatattttt tcaatgatta aacccatgta tggtaagctt ttcataactt tgtttttata
     8761 ttaacaaatg atttatatta gtgcaatagc atattataaa ttgttatctc atcataacac
     8821 acatgccctc aattctttta tacacttcag tccaaagaaa tttttcgtca gcagaaaaat
     8881 atatccctct ctatatttat tataatgctc tcgaaaactc gtattgcttt cgaggtctct
     8941 atttcaggag
//
LOCUS       DAOOJC010000037         8863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_6894_length_8863_cov_3.789623, whole genome shotgun sequence.
ACCESSION   DAOOJC010000037 DAOOJC010000000
VERSION     DAOOJC010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6894_length_8863_cov_3.789623"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..734)
                     /locus_tag="PLA01_01910"
     CDS             complement(3..734)
                     /locus_tag="PLA01_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HPD00087.1"
                     /translation="MDKQISHGNNKTCTKCTSILWEKYHAGIIPVIPDIKCKSPAEVD
                     LLMGRDPVSIACSLEAAGAPVISVVTESEHYGGSKKLLNSIAKAVRVPILRKDFIINR
                     EQLLESVEMGASSVLLISSILEKKQLFRLVEEAVLLGLEPLVETHSEEEIKAANEMDI
                     TFLGINNRNIIQWETDDGNVNTTERLAGLARSGAFLLSESSISSSEDVKRAIAAGAHS
                     VLVGTAILKAEDPAKMYLVLSGQEV"
     gene            complement(736..1767)
                     /gene="trpD"
                     /locus_tag="PLA01_01915"
     CDS             complement(736..1767)
                     /gene="trpD"
                     /locus_tag="PLA01_01915"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPD00088.1"
                     /translation="MIRNVLKKLTAREDLTYEEAYSLIVAIKNDELSDVQIAGFQVAL
                     LMKGPTLTEITAIAQAMRDNCIKISPDVDDELMDTCGTGGGLSTFNISTAVAFVAAAA
                     GIPVAKHGSRSISSLSGSADVLEALGIEINQSPKSVEKLIEEVGIAFIYAPLFHPVMG
                     KVLPPEKDLGIKTIFYTIIGPLINPSQASRHVMGVYKPELLDTVSKVAASIGYKRALF
                     VHGLDGLDEISLMGKTRINELNNGNITVYEIAPEDFGLSRCTLEEIATGTPQENACMI
                     RDVFSGKDKGPRRQAVVLNAAGALMIGNKAASFKEGIQLAGEIIDSQAAKSKLNQLIE
                     ASNEYKSVV"
     gene            complement(1853..2623)
                     /gene="trpA"
                     /locus_tag="PLA01_01920"
     CDS             complement(1853..2623)
                     /gene="trpA"
                     /locus_tag="PLA01_01920"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPD00089.1"
                     /translation="MNKLQRAFSNGKAFIPFITAGDPSLEITEQLVLKMAEAGADLIE
                     LGIPFSDPVAEGPVIQEADYRALSAATTTDKIFDMVKNIRKSCDVPLAFMTYANPVFT
                     YGAERFMKKSQEVGIDAVIIPDVPFEERDEFLPYCSKYGIEFISMIAPSSRERIRMIA
                     REARGFVYCVSSMGVTGVRAEIGNDAEDMIKQVKEVKDIPCAIGFGISTPEQAARMSK
                     FSDGVIVGSAIVKIVGQYGIDCIPHVVDYVRKMKSAVK"
     gene            complement(2616..3800)
                     /gene="trpB"
                     /locus_tag="PLA01_01925"
     CDS             complement(2616..3800)
                     /gene="trpB"
                     /locus_tag="PLA01_01925"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPD00090.1"
                     /translation="MPKGRFGVHGGQYIPETLMNAVIELEEAYNRFKNDPGFVAELNE
                     LLENYAGRPSLLYFAGKMTKDLGGAKIYLKREDLNHTGSHKINNVLGQVLLAKRMGKT
                     RVIAETGAGQHGVATATAAALLGLKCEIFMGKEDTERQALNVFRMKLLGAEVHAVTTG
                     TQTLKDAVNETMREWTKRVHDTHYVLGSVMGPHPFPTIVRDFQSVIGREVKEQLMKKE
                     GRLPDAVLACVGGGSNAMGLFYDFINDTGVRLIGCEAAGKGVETDKNAATIANGKPGI
                     FHGMKSYFCQDEYGQIAPVYSISAGLDYPGIGPEHANLKDTGRAEYVPVTDDEAVKAF
                     EYLSLVEGIIPAIESAHAVSYAMKLAPTMGKDQIIVICLSGRGDKDVAAIARYKGEQI
                     YE"
     misc_binding    complement(3912..4161)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4284..4847)
                     /locus_tag="PLA01_01930"
     CDS             complement(4284..4847)
                     /locus_tag="PLA01_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010077557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPD00091.1"
                     /translation="MGNIELLIIAVGLSMDAFAVSICKGLSMKKMSYKNAVITGCFFG
                     GFQALMPLLGYLLGKQFEKYIVSIDHWIAFILLSIIGINMIKESRNTCEIPDDSFNIK
                     SMTLLALATSIDALAIGVTFAFLQVNIIPAVAMIGIITFSFSFAGVKIGNLFGAKFQS
                     KAETVGGAILIAMGLKILLEHLGIINF"
     gene            5180..6169
                     /gene="sppA"
                     /locus_tag="PLA01_01935"
     CDS             5180..6169
                     /gene="sppA"
                     /locus_tag="PLA01_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPD00092.1"
                     /translation="MKNKQLIALVVAGLVFVFVCSSSVLVNTISKQFESSLNLNSFSL
                     SGNVLPVTPHIGVVKVEGTIMDSGSTSLFSSEGYNHQNTLDLIEDLKNSSSNKGILLY
                     VNSPGGTVIASDDLYLKLKEYTEETGRPVWTYMADQACSGGYYISMASEKIYANRNTW
                     TGSIGVLISLMNYKGLYEKIGLKGIYITSGNNKAMGAGDLDLTDEQQQILQSLVDESY
                     DQFVGIVAEGRNMTVSQVKKIADGRIYSAKQALDNKLIDEIATYEEMKEAFSKELDDA
                     EIYTPETNDLFNFGSLFSNMSKLKPRSDAEMLIEFMDKNGSGVPMYYAEPGLF"
     gene            6147..6644
                     /locus_tag="PLA01_01940"
     CDS             6147..6644
                     /locus_tag="PLA01_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008427231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPD00093.1"
                     /translation="MQNQDCSSTTIYAGFAVRLMAFIIDSLLVGAALMIVRIPVFFIS
                     LIDPSNILKVPVLFKFSVYDIVLYLLGATYYVLMTYYCGFTLGKKLLNLKVVSDNEEG
                     LTFINVLYRETIGKYLSAILFIGYLMIAVDSEKRGLHDRLCDTRVIYNFKIPVKVVTE
                     EQPIN"
     gene            complement(6673..6873)
                     /locus_tag="PLA01_01945"
     CDS             complement(6673..6873)
                     /locus_tag="PLA01_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HPD00094.1"
                     /translation="MENKEKDKKQLVLRLSSSLWKELVAWAEDDFRSLNGQIEYLLTE
                     CVRHRKKSKDQKGDQTGDGSLF"
     gene            complement(6902..7942)
                     /locus_tag="PLA01_01950"
     CDS             complement(6902..7942)
                     /locus_tag="PLA01_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPD00095.1"
                     /translation="MSDIKLNERNMQYEEIIAKSRNGMTVLLLNILLMLGSVACFIWG
                     VTLVDSESNVKMGIVLIVVGALYFFLIGPIVFAGLKVLKPNEALVLTLFGKYYGTLKG
                     EGFFFANPFVSAVNPAAVSPVSTGNIVDEKAAMLAKDAPQSAMVSFVSKKISLKTMTL
                     NNDKQKINDQLGNPVIIGVVVIWRVVNTAKAVFNVDNYKEYLSIQCDSALRNIVRLYP
                     YDISLEDENEKSLRGSSIEVADKIKQELQSKVDIAGLEIIETRITHLAYAPEIASAML
                     QRQQATAIIDARQKIVEGAVGMVEMALAKLSENDIVNLDEERKAAMVSNLLVVLCGNR
                     DAQPVVNSGSLY"
     gene            8261..>8863
                     /locus_tag="PLA01_01955"
     CDS             8261..>8863
                     /locus_tag="PLA01_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HPD00096.1"
                     /translation="MFPITTQTIQIVSLIISALALGVAAWLYTWVKAQPSSNKRIAEI
                     GEYIRQGANTFLKREYLVLARFTAVAAILIAILLPKPIWTGNNILDNILMAVSYVFGT
                     VLSALAGKIGIQIATIANIKAAEAAQKGVKPSFMAGFRGGAVMGMAVVGTSLLGVTLV
                     FMIIGDASALLGFSFGASSLALFAKAGGGIFTKTADISADL"
BASE COUNT         2532 a         2083 c         1580 g         2668 t
ORIGIN      
        1 tactaaacct cctgcccgct aagtacaaga tacattttcg ccggatcttc cgccttcaaa
       61 attgccgttc ccaccaagac cgaatgagct ccggctgcta tggctcgctt cacgtcctcc
      121 gaagaagaaa ttgaactttc acttaataga aaagccccgg aacgtgccaa tcccgcaagt
      181 ctttctgtag tatttacatt tccgtcatcc gtttcccatt gaattatgtt gcggttgttg
      241 atacctaaaa aagttatatc catttcattg gctgctttaa tctcctcttc gctgtgagtt
      301 tccaccagag gctccaaccc taataagact gcttcctcca ccagtctgaa caactgcttc
      361 ttttcaagta ttgatgaaat cagtaaaacc gaactagctc ccatttcaac actctctaga
      421 agctgttccc ggttaattat aaagtccttt cgtaaaatgg gcacacggac agccttagct
      481 atgctgttca gtaatttctt ggagcctccg taatgctccg attccgttac caccgagata
      541 acaggggcac cggctgcttc caaagaacag gctatggaga caggatccct tcccataagc
      601 aagtcaacct ccgccggcga cttgcacttt atatccggta ttacaggaat tatacccgca
      661 tggtattttt cccacagtat tgaagtacat tttgtacacg ttttgttatt tccatgactt
      721 atttgtttat ccatatcaaa ccaccgattt atactcattt gaagcttcaa taagctgatt
      781 cagcttgctt tttgccgctt ggctgtcaat aatctcgccg gcaagctgta ttccttcttt
      841 aaaggaagct gccttattgc caatcatcaa cgcaccggct gcatttaaaa caactgcctg
      901 acgtcttggg cctttatcct ttcccgaaaa aacatctctt atcatgcagg cgttctcctg
      961 gggcgttcct gtcgcaattt cttcaagagt acagcgggac aagccaaaat cctccggtgc
     1021 aatctcatag acggtaatat ttccgttatt gagctcgtta attcttgttt ttcccatcaa
     1081 tgaaatttca tccaaaccat ccaatccatg cacgaacaac gcccttttat aacctatgga
     1141 agcagctacc ttagaaaccg tatccaaaag ctccggctta taaaccccca taacatgacg
     1201 ggaagcctgt gaaggattaa tcaacggacc gattatcgta tagaaaattg ttttaatacc
     1261 caaatccttt tccggaggaa gcacctttcc cattaccgga tggaatagcg gtgcataaat
     1321 aaatgcaatc cccacctctt caatgagctt ttcaaccgat ttggggctct gattgatttc
     1381 tatacccagg gcttccagca catcggcact tcccgaaagg cttgatatgg accggcttcc
     1441 gtgcttggca acaggaatac ccgcagccgc tgcaacaaag gccactgctg tggaaatgtt
     1501 aaacgtgctc aaacccccac cggttccaca ggtatccatt agctcgtcat ctacatcagg
     1561 agagattttt atacaattat ccctcatagc ctgggcaata gcagtaattt ccgtcaatgt
     1621 cggtcccttc attaataatg ccacctgaaa accggcaatt tgaacatcac tgagctcgtc
     1681 attttttata gcaacaatca aactgtatgc ttcttcatag gtcaggtctt cccttgccgt
     1741 caactttttt aaaacatttc taatcatatc gattcctcct attttccgga ttctgcactt
     1801 tccatagagg caaaacccaa tttgttaatg atgggtattc atattttttg ccttatttaa
     1861 ccgcactctt catttttcgt acataatcaa caacatgagg tatacagtct atgccgtatt
     1921 gccctacgat tttgacaatg gcacttccca caattactcc gtcggaaaat ttgctcattc
     1981 ttgctgcctg ctccggtgtt gatatcccga aaccgatggc acaaggaata tccttaacct
     2041 cttttacctg ctttatcatg tcctctgcat catttccgat ttcagcccgg actccggtaa
     2101 ctcccataga tgatacacaa tacacaaatc cccgggcttc ccgggcaatc atccgtattc
     2161 tctcacggga tgacggagca atcattgaaa tgaactcaat accgtatttt gaacaatagg
     2221 gcagaaattc atccctttct tcaaagggaa catcgggaat aataaccgca tcaattccca
     2281 cctcctggct ctttttcata aacctctcgg caccgtaagt gaaaacggga ttggcataag
     2341 tcataaaggc caaaggcaca tcacacgact tacgaatatt ttttaccatg tcaaaaattt
     2401 tatcggttgt agttgcggca gaaagtgcac ggtagtcggc ctcctgaatc accgggcctt
     2461 ccgccaccgg atcggaaaag ggtataccca gttcaatcaa atcggcaccg gcttctgcca
     2521 tctttaaaac caactgttcc gttatttcca aggaaggatc ccccgcagtt ataaacggga
     2581 taaaagcctt accgttacta aaagccctct gtaacttatt cataaatctg ttccccctta
     2641 taccttgcta ttgcagcaac atccttatct cctcttcccg aaagacaaat tacaatgatt
     2701 tgatccttac ccatggtcgg agccaacttc attgcatagg atactgcatg ggcactctca
     2761 attgccggta tgattccctc aaccagtgaa aggtattcaa aagcctttac agcctcatca
     2821 tcggtaaccg gcacgtattc ggccctgcct gtatccttca ggtttgcatg ctccggacct
     2881 attcccggat aatcaagccc tgccgatatg gaatacactg gtgcaatctg tccgtattca
     2941 tcctgacaga aataggactt cattccatgg aaaatacctg gctttccgtt tgctattgtt
     3001 gccgcattct tgtccgtttc aacacctttt cctgccgctt cgcacccaat caggcgaaca
     3061 ccggtgtcat taataaagtc gtagaataat cccattgcat tgctgcctcc cccgacacat
     3121 gccaataccg catcggggag ccgcccttct tttttcatta actgctcctt aacctctcgg
     3181 cctatcacgc tttgaaaatc ccggacaatg gtgggaaacg ggtgcggtcc catgacagag
     3241 cccaacacat aatgagtatc atgaaccctc ttagtccact cacgcattgt ttcatttacc
     3301 gcatccttca gggtctgagt tccggtggtt accgcatgaa cctcagcccc taaaagcttc
     3361 atccgaaata cattcagtgc ctgtcgttcg gtgtcctctt tgcccataaa tatctcacac
     3421 ttcaacccta aaagtgcagc cgcagttgct gttgccaccc catgctgtcc ggctccggtt
     3481 tcggctataa cccgggtttt ccccatgcgt ttggcaagga gcacctgccc caaaacgttg
     3541 tttattttgt gggatccagt gtggtttagg tcctcccgct ttaaataaat ctttgcccct
     3601 ccgagatcct ttgtcatttt gcccgcaaaa tagaggagcg atggccgccc ggcataattt
     3661 tccaacaatt cattcagctc cgcaacaaac cccggatcgt tcttaaagcg attgtaagct
     3721 tcctccagtt caattacagc attcatcagt gtctcaggaa tgtactgacc tccatgtact
     3781 ccaaatcttc cttttggcat atgcgtcact ctcccataat aaaagtccaa tcaaagggca
     3841 tcgagccctc accgcttttt ggtcatttca tttcgtgaaa tctcacgaaa taaaaaaagc
     3901 tttcatccct gttaagggac aaaagcattt gacttctgtg gtaccaccca aattgacgga
     3961 aaaccgtcca ctcattttgc atactaacat atgcacccca ctgataacgg acagggttcc
     4021 cgtaagtgcc tactctcgca acaggatttc ggacttccct ctcaagccca ttcagaataa
     4081 cctctataat cgcattccca ccagctagcg actctctttg tacaggtaat tatccctact
     4141 cttcttgatc ataggtttac aactagtatt aaacaaatta tagcatttat catatacctc
     4201 tgtcaactgc aattattgta attttaagtt tatacattta tatactcatt attactgctt
     4261 acctgaatat ttatcttcgc attttaaaaa ttaattatcc caaggtgttc caaaagaatc
     4321 ttcaacccca tagcaatgag tatagcaccg cccacagtct ctgcttttga ctggaacttt
     4381 gccccaaaca ggtttccgat cttaaccccg gcaaaagaaa agctaaaggt aatgattcca
     4441 atcatggcaa cagcgggaat aatgttcacc tgtaaaaaag caaaggtaac acctattgcc
     4501 aaagcatcaa tacttgtagc cagagccaac agcgtcatac tctttatatt aaaggaatca
     4561 tcaggtatct cacaggtgtt tcgggattct tttatcatat tgattccgat aattgataga
     4621 agaataaaag caatccaatg gtcgattgat actatatact tctcaaattg cttccccaaa
     4681 agataaccca aaaggggcat taacgcctga aatcctccga aaaaacagcc ggtaataaca
     4741 gcattcttat agctcatttt tttcatcgac aatcccttac aaatcgatac tgcaaaggca
     4801 tccattgaga gtccgactgc tatgattaat agttctatat ttcccaaatc atcattctcc
     4861 ttcctgattt tttattataa catgaatctg acaagtatca agaagaattc tagtattccc
     4921 cagtttttta taaggatctt ttttcctaac aggatactga atgaattggg ttaacagtta
     4981 tgtacgccgt aaactaacaa atctataaac ataaatattt taaaataata cttattaaaa
     5041 atatgcttca tataataatg atgtatattt atatcacaca tgtttatgta aaataattgc
     5101 ttgaatttat cctttactgc aataacaaag tatgcgataa taaattttgt aaaaaatctg
     5161 tgaaaagtga ggtcgaaaaa tgaagaacaa acaacttata gctttagtcg tagcagggtt
     5221 agtatttgta tttgtatgct cttcaagtgt tttggtaaac accatatcaa aacaatttga
     5281 atcatccctg aatttgaata gttttagtct ttccggcaat gttctgcctg taaccccaca
     5341 tatcggtgtg gtaaaagtgg aaggcaccat tatggattcc ggaagcacca gtttgtttag
     5401 cagtgaagga tataatcatc aaaacactct ggatctcatt gaggacctga agaattcatc
     5461 aagcaataaa gggattttgt tgtatgttaa ttctcccggt ggtactgtta tcgccagtga
     5521 tgatctctat ctgaaattaa aagaatatac cgaagaaacc ggaagacccg tctggacata
     5581 tatggcagat caggcatgct ccggcggtta ctatatatcg atggcttccg agaaaattta
     5641 cgcaaacaga aacacctgga ccggttctat tggcgttctc atctccttga tgaattataa
     5701 aggcttatat gagaaaatag gacttaaagg aatttatata acaagcggca acaataaggc
     5761 aatgggtgcg ggcgaccttg atttaaccga tgaacagcaa cagattctac agagtcttgt
     5821 ggacgagtct tatgatcagt ttgtcggaat tgtagcagaa ggcagaaata tgacagtgag
     5881 ccaagtcaag aagatcgctg atggcaggat ttactccgca aagcaggcgt tggacaataa
     5941 acttattgat gaaattgcaa catatgaaga aatgaaagaa gctttcagta aagaacttga
     6001 tgatgctgaa atatacacac cggaaaccaa tgatttattt aatttcggtt cattattcag
     6061 taacatgagc aaattgaagc cacgttcgga tgcagaaatg ctcattgaat tcatggacaa
     6121 aaacggaagc ggggtgccaa tgtattatgc agaaccagga ttgttctagt actaccatat
     6181 atgcaggatt tgcagttcgt ttaatggctt tcattattga ttcattgctt gtgggagctg
     6241 cacttatgat tgtgcgtata ccggttttct ttatttccct tatagatccg tcaaacattt
     6301 tgaaagtgcc cgtgcttttt aagttcagtg tttatgatat agtgctttac cttttgggtg
     6361 caacgtatta tgttttgatg acttattatt gcggctttac tctggggaaa aaactgttaa
     6421 atttaaaggt agtaagcgat aatgaagaag gattgacctt tatcaatgtt ctataccgtg
     6481 aaacaatcgg aaaatattta tctgcgattc tttttattgg ctatttaatg atagcagttg
     6541 acagtgaaaa gcggggtctt catgacaggc tttgcgatac cagagtcata tacaatttta
     6601 agattccggt taaggtagtg acagaagaac aacccatcaa ctaagacata gaacagggga
     6661 cagttctctg ttttagaaca gagaaccgtc ccccgtttga tccccttttt gatctttact
     6721 tttctttcga tgcctgacac actcagtcaa taaatattca atctgcccgt tcagtgaacg
     6781 gaaatcatct tctgcccaag caactaattc tttccaaagg gaagacgata gtcgcaagac
     6841 cagctgcttc ttgtcttttt ccttattctc catctatcat catcccaccc ttccattctt
     6901 atcaatataa tgaaccactg tttacaaccg gctgagcatc cctgtttccg caaagaacaa
     6961 ccaataaatt gctgaccatt gctgcttttc tctcttcatc aagattaact atatcatttt
     7021 cactgagctt ggcaagagcc atttccacca ttccgacagc accctcaaca atcttctgtc
     7081 ttgcatcaat aatagctgtt gcttgctgtc tctgtaacat agcagaggca atttccggtg
     7141 catatgccag atgagtaatt ctggtctcaa ttatttcaag tcctgcaata tccacctttg
     7201 attgaagctc ctgcttaatc ttatctgcaa cctcgatact gcttccacga agagattttt
     7261 cattttcgtc ttccaaggat atgtcataag gataaagccg tacaatatta cgcaatgcgg
     7321 aatcacactg gatggagaga tactctttat agttgtccac attgaaaact gcttttgcgg
     7381 tattaacaac cctccagatt accacaaccc caatgattac aggatttcct aattgatcgt
     7441 ttattttttg cttatcattg ttcagcgtca tggtcttaag tgagattttc ttgctcacaa
     7501 aagataccat tgcactctgt ggtgcatcct tagcaagcat tgccgctttt tcatcaacaa
     7561 tattaccggt agatacggga gacactgcag ccggattgac agctgaaaca aagggattgg
     7621 caaagaaaaa accctcaccc ttcaatgtgc cgtaatactt gccgaaaagc gttaacacaa
     7681 gagcttcatt gggtttaagt actttcagcc cggcaaacac tatgggacca ataaggaaaa
     7741 aatataaagc ccccactaca atcaaaacta tacccatttt aacgtttgat tcgctgtcta
     7801 caagtgtaac accccagata aaacacgcta cggaccccag cataagcaaa atattcaata
     7861 aaagaaccgt catgccgttt ctgctctttg cgatgatttc ttcatactgc atgtttcttt
     7921 cattaagctt aatgtctgac atttataaaa cccctttctt taaaataata cagtctagta
     7981 tcattttgat atcatattgt tttcacttta atattaccat gtattttttc gcatgtcaat
     8041 attctctatc tgataaggta cattatataa atgtaaggaa aagtattcca gttgcttata
     8101 gtattggtac atttttcctt gtctaaaccc atacatatat ttaaaacagc ttaaataaaa
     8161 gttcaaatat atttaataac tagtttcctt ttttaacagt tataatgtat aatatacaat
     8221 ggtaattaaa agatataaga aatgttaagg aggttcatca atgtttccaa tcacaacaca
     8281 aacgattcaa atcgtttcgc tcatcatctc tgccctcgct cttggtgttg ctgcatggct
     8341 gtacacatgg gtgaaggctc aaccatcttc caataaacgt attgctgaaa ttggtgaata
     8401 tattcgccaa ggtgccaata cgtttttaaa acgtgagtat ttagtactcg cacgttttac
     8461 cgcagtagca gctattttga ttgcaatatt gttgcccaaa ccaatctgga caggcaataa
     8521 tattcttgat aatatcttga tggctgtctc atatgtattc ggtacggttc tgtccgccct
     8581 tgccggaaag atcggtatcc aaatcgcaac tattgccaat attaaggctg ccgaagctgc
     8641 acaaaaaggc gttaagcctt cttttatggc aggtttccgc ggcggtgcgg taatgggtat
     8701 ggctgttgtt ggcaccagcc ttttaggtgt cacacttgtg tttatgatta ttggagatgc
     8761 aagtgcactt ttgggattca gtttcggtgc aagttcgctg gcactttttg ccaaagcagg
     8821 cggtggtatt ttcacaaaga ctgccgacat cagtgccgac ctc
//
LOCUS       DAOOJC010000038         8860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_6896_length_8860_cov_3.996366, whole genome shotgun sequence.
ACCESSION   DAOOJC010000038 DAOOJC010000000
VERSION     DAOOJC010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 8860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8860
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6896_length_8860_cov_3.996366"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLA01_01960"
     CDS             <1..242
                     /locus_tag="PLA01_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00097.1"
                     /translation="KKYLTYTLFGLNNTPLFSYDSNTMNSEAKKAYLDVLASNSKDSG
                     YLKILSGFMSLLEENGYKLTGEVEEYWDEVMEKLD"
     gene            435..968
                     /locus_tag="PLA01_01965"
     CDS             435..968
                     /locus_tag="PLA01_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPD00098.1"
                     /translation="MFQEEKLQKAHDGDLKAIEEICSLTWEPVYRFVYYKVQNRQEAE
                     DITQEAYVKAFTYLQRNDVKIEKFIGFLKTTALNILRDKWRKKKRLGSKVNYDEISPA
                     ETAVDDATEASTQRTVIQDAMNKLNGEQRTVIELRILKGYSTYETAKIMNKKEVTVRV
                     LQYRALKNLAAILKSYD"
     gene            994..2670
                     /locus_tag="PLA01_01970"
     CDS             994..2670
                     /locus_tag="PLA01_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HPD00099.1"
                     /translation="MVNDEKRLSDYIDRLNEEKKPKAHENSAESSEMEELMKTVRKVR
                     SLKEPTLPGLDYPGKLAENVAKQLSSKPAVKNKKWTWIAATAAVAAVVTLVLTLNFVL
                     HPGGIDIVYAMEQAFQEVKAYHGVIEIIEKNAEGQKTTQAIREVWADKEGHYYVKELY
                     GSQTGLVTVNNGEKKWQQRTDEKQVFIFPAFPDPYRFTFELGNEIDDVKNAAVVKTIG
                     EDSVSNREAAVLEVTPKGGQPYRIWIDKETKLPLKRESAMFNSIQYTVTYMSIEYCDV
                     LPKELVAYSIPAGFEEIDKNPEQLVNSIEEVEGMTGFTPKMPKEIPQGYERDSIAVAN
                     GMKTVKLNYSSPDKRNRIIILQGKEEGELNPASTAVLGTVNGNVAEIQSPVQGDYGII
                     AGGGVYSGITDISSIRWQEGGFEFAVVGNVSPENLALFAESLTGGPVVIPKGDEKTYD
                     KPQIKVPVDLEIEENEQKSADAGHSPWKLDPVYVAQVFASLKISPEGIQGDYPVPYED
                     IRLIKNDGVDAVAEISTAKATVRRVYLKRLIRQDSTGIWTVVGYDPVSPE"
     gene            complement(2911..3132)
                     /locus_tag="PLA01_01975"
     CDS             complement(2911..3132)
                     /locus_tag="PLA01_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00100.1"
                     /translation="MAEFCICGSLMLDGSCTNKNCSSRASAKVVSSAKRAGKGTASKK
                     ETSTRTRTRRASKCITYNLSDEIEKVENS"
     gene            3404..4336
                     /locus_tag="PLA01_01980"
     CDS             3404..4336
                     /locus_tag="PLA01_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD00101.1"
                     /translation="MFKKKILIIDDTEFMTKLISDILISSDYDVVIASNGPTGLQKVR
                     TEKPDLVILDVVMPGMDGFEVCRILREDESNNLMPIIMLTAQENEDDKLTGLELGADD
                     YIIKPFNSRELVSRVRNTLKRIDRNRWANPLTGLRGNIEIQTEINQRIATKQHFAVIY
                     ADLDNFKAYNDVYGFASGDRAIKLTADIIIDNVHNCGNPEDFIGHIGGDDFIIVSTPN
                     KAELVCKGIINDFDTKIKSLYCDEDLKRGYIVTSNRQGQIMKFPLISISLAIVSNEMR
                     DLISHIQISEIAAELKKKAKSMPGSTYVKDRRKS"
     gene            4443..6077
                     /locus_tag="PLA01_01985"
     CDS             4443..6077
                     /locus_tag="PLA01_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HPD00102.1"
                     /translation="MRLKRSHAFLLTVFICITLVLPGCKNVEADYNGICLNLGDEPLT
                     LDPQLVFDVVPMRVINAVFEGLCRKDKEGMPVPGAAEHWEISEDKLTYTFYIRKDAKW
                     YNGASVTAKDFKDAWMRALDPEPQYHEPAYMANLFFCIEGAEEYAYGEGKPEDVAIDA
                     VDDRTLRVTLVQPTPYFLDLVCNSVYMPVNKEFFDQQISGDISEYGTEAETILGNGPF
                     MVEKWNHDESIVLKKNNAYWNSDKISIEKIEFKMIKDNTAAVNSFKAGEIDMVEINDH
                     LLADELKNSNCRVETYDAGIVQYITINNEDPVLCNVNIRKSLSYALDRKTLVEKVLND
                     GSKEAFGFVSPVVAGYEGKFRKQAGDLYKDNDKDTARKLLEKGLDELGLSEMPKITFL
                     INDQEISKRDAQVLQDMWKKSLDVDVELQVMSFDAMTEKMLSKDYQMALLMWSADYND
                     PLAYLDIFSSNNFYNVAYYHNKQFEELLAESIEETDVKKRMDLLLKAEKIAIDDMAVC
                     PLYFVANSYAVKSRVKGLVRGSSAIQDIDVYWTYVE"
     gene            6170..7141
                     /locus_tag="PLA01_01990"
     CDS             6170..7141
                     /locus_tag="PLA01_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD00103.1"
                     /translation="MRFLLKRLAFFSVTMWLVVTLTFVLIRCLPGSPFNTDKMIHPQI
                     LENLNTKYGLDKPFYEQYLRYIQNLVRGDLGISMIYKNRSVASIIKRAFPVSLDLGVR
                     AIILAAFAGLFLGLISTLYSNKAADHVISAITMIAVSVPGFVAGALLQYFLCYRLSVL
                     IQSITQVGYSVFPNTGWEGFRSTVAPTMALAAGVMGMVVKMLRTSMKDVLDQNYIVMS
                     RAKGLNKREVVLRHALKNALLPLLSISGPLVSSIIMGSFVIENIFSIPGLGRYFVSSV
                     QDKDYTMVLGLAVFSVFIVVLINTITDILYAVVDPRVCIESKDRLKT"
     gene            7177..8022
                     /locus_tag="PLA01_01995"
     CDS             7177..8022
                     /locus_tag="PLA01_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD00104.1"
                     /translation="MLLYVKTAVFGVLKNKIALAGAVIFFVIVMMAIAAPAFSPYNYY
                     SHDADSIFLKPMSEGHWFGTDEFGRDLFVRVWMGARISLAIGILAAIIQMVIGTLYGG
                     MAGLFGGLADEAMMRLADALYSIPDLILIVLVTLMMGNSGFSVVLALSLTGWTSTARV
                     VRGQTLMLKEMDFVKAAKMFGAGRWYIMFKHILPNCSGQILASLMLNVPSAIFAESTL
                     SFLGLGIRPPEASWGTLVNEGFKYSNITYHPWVVFLPLLFIAITMISLNILGDEFKKV
                     LDSKH"
     gene            8044..>8860
                     /locus_tag="PLA01_02000"
     CDS             8044..>8860
                     /locus_tag="PLA01_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD00105.1"
                     /translation="MDTILKIKDFSLSIKGKKILDNINMSVSKGEILAIVGESGSGKS
                     MLARSILRLNSPDFFDIKGEIFFRDQDINSLPEKQLTKIRGAKIGMVFQEPLSYLNPV
                     IKMASQLTEPLTKHKKVNKRKAAELMEDLLRKLNINNPEQYMKRYPHQLSGGMAQRGV
                     IAMTSSCEPELIIADEPTSSLDVIIQFSITKLIKDLAKLLGVSVVFITHDLRLAAKIA
                     DRVAVISKGRIVEEGDVQEVLKNPKSEETAQLLNACIVSGGRQIGAKSKVLLEI"
BASE COUNT         2747 a         1513 c         2149 g         2451 t
ORIGIN      
        1 ataaaaaata tttgacatat acattgtttg gcttaaataa tactcccctg ttcagctatg
       61 actctaacac gatgaattct gaggctaaaa aggcttattt ggatgtattg gcatcaaact
      121 caaaggatag cggttatctt aaaattctaa gcggctttat gagcctgctc gaagaaaacg
      181 gttacaaact aacgggtgag gtagaagaat actgggacga agtaatggaa aagctggatt
      241 aacaggggag tttaaaatac agaaatgggg gtctttatgg ctcccatttt tttatgagat
      301 ttgatgtagg aaatttttca caaacgaaaa atttccttcg tattgaagtg ttatatagtg
      361 taacgtttat tacgacttaa gcgtcattat tattgtacgc acaactgacc ggttggtgaa
      421 aaggagctaa gaatatgttt caggaggaaa agctgcaaaa agcacacgat ggagatctaa
      481 aggcgataga agaaatatgc tctttaacat gggaacccgt ataccgtttt gtatactata
      541 aagtgcaaaa ccgtcaggaa gcggaggata ttacacagga agcctatgta aaggcattta
      601 cctatcttca gagaaacgat gtaaaaatag aaaaatttat tggcttttta aaaaccacag
      661 cattaaatat tttgcgggat aagtggagga agaaaaaacg cttgggttca aaggtaaatt
      721 atgacgagat aagccctgca gaaacagctg tggacgatgc tacggaggcc agcacccaga
      781 ggacggtaat acaggatgca atgaacaaac ttaatggaga gcagcggacg gttattgagt
      841 tgcggatact caagggctat tccacatatg agactgcaaa aatcatgaac aaaaaagaag
      901 tcacggttcg tgtgctgcag tacagggcat tgaaaaacct tgctgcaatc cttaaaagtt
      961 atgattaatt atggaagttg gaggtataga attatggtaa atgatgaaaa aaggctgtct
     1021 gattatattg acaggctaaa tgaggagaag aaacccaaag cccatgaaaa ttctgcggag
     1081 tcttcggaaa tggaggagct tatgaagaca gtgagaaaag ttcgcagttt aaaggagccc
     1141 actcttccag ggctggacta tcccgggaag ctggccgaaa atgtggcaaa acagctatcg
     1201 tcaaagccgg cggttaagaa taaaaaatgg acttggattg ctgcaacggc agcggtagcg
     1261 gcagttgtta cactggtact tacactgaat tttgtattgc accccggagg cattgatatt
     1321 gtttatgcta tggaacaagc ctttcaagaa gtaaaggcat accatggggt tattgagatt
     1381 attgagaaga atgccgaggg acagaagacc acgcaggcaa tccgtgaggt atgggccgat
     1441 aaggaaggac actattatgt aaaagagctt tacggctcgc agacgggcct ggtaacggta
     1501 aacaacggag aaaagaaatg gcagcagcgt actgatgaga aacaagtgtt tattttccca
     1561 gcatttcctg acccgtaccg atttactttt gaactgggta atgaaataga cgatgttaag
     1621 aatgccgctg tggttaagac tatcggagaa gacagtgttt caaacaggga agctgcggtt
     1681 ttggaggtca cacctaaggg cggacaaccg taccgcatat ggattgataa agaaaccaaa
     1741 cttccgctta agcgggagag tgcaatgttt aattcaatcc aatatacagt gacttatatg
     1801 agcattgaat attgcgatgt tttgcccaag gaattggttg catatagtat tccggcggga
     1861 tttgaggaaa tagacaaaaa ccctgaacaa ttggttaaca gtattgaaga agtagagggc
     1921 atgaccgggt ttactcccaa aatgccaaaa gaaataccac aaggttacga aagagacagc
     1981 atagcggttg ctaacggtat gaagacagtc aaactcaatt atagttcgcc tgataaacga
     2041 aaccggataa ttattttgca gggtaaagaa gaaggagaat taaatccggc atcaacagcg
     2101 gttttaggca cggttaacgg aaatgttgcc gaaatccagt cacctgttca aggggattat
     2161 ggaatcattg cgggtggagg agtttattcc ggtataacgg atatttcatc tattcgttgg
     2221 caggaaggcg ggtttgaatt tgctgtagtt ggaaacgttt cgcctgagaa cctggctttg
     2281 tttgcagaaa gcttaaccgg cggtccggtg gtgattccaa aaggggatga aaaaacttat
     2341 gataagccgc agattaaggt tcctgttgat ctggaaattg aagaaaatga acagaagagt
     2401 gcagatgccg ggcattcccc gtggaaactt gatcccgttt atgttgccca ggtatttgca
     2461 agcctcaaga tttctccgga aggaattcag ggagactacc ctgtcccgta tgaggatatt
     2521 aggctaataa aaaatgacgg tgtggatgct gttgcggaaa taagtacagc aaaagcaacg
     2581 gtacgcaggg tgtatttaaa gagactgata agacaggaca gcacgggaat atggactgtg
     2641 gtaggatatg accccgtttc gcctgaataa gatgcaaaag gattcttaaa tcctgtgata
     2701 ttgaagaaac attgtgaagc aagttataga tgcctgtcaa tacgttgtgg aaagggctag
     2761 catcgttcga atgcagtaag tgagttttgc cctaccccac gaagtattca caggctctgt
     2821 acttgtgaaa ttaaagtttt cttttcatat ttaataaatg cacctgccgc ataccgggcg
     2881 gtatgagcgg aggtgagaga aaagaaaaaa tcagctgttt tctacttttt caatttcgtc
     2941 acttagatta taagtgatgc attttgatgc tcttcgggta cgagtgcggg tggatgtttc
     3001 cttttttgaa gccgttccct ttcctgccct ttttgccgaa gagacaacct ttgccgatgc
     3061 ccggctgctg caatttttat ttgtgcagct gccgtccagc atcaacgaac cacagataca
     3121 aaattccgcc aattaaatca ctcctttaat agtaattata ccacaaaaaa tgaaatctta
     3181 tatgtttcct aaaaaacatt taagtgtata gtatttaatg tgaaaaaatg cggaggcagt
     3241 attttgtatc aaaaaatttc aaaattgata ttaattttca caagtcaact tttcaaatac
     3301 tcttttttaa tatataattg aataacaacg ttattattta ttcttgttat tccgatatat
     3361 attgcaggta ttataattat tcttcaatag gagctcacaa tcgatgttta aaaaaaagat
     3421 tttaataatt gatgataccg aattcatgac aaaattgatt tcagatatct taataagttc
     3481 agactatgat gttgtgattg catctaatgg tcccacaggt ttgcaaaagg tcagaacgga
     3541 aaagcccgac cttgttattc ttgatgttgt tatgccgggt atggatggct ttgaggtttg
     3601 cagaattttg agggaagacg agagtaataa cctgatgccc attatcatgc tgacagcaca
     3661 ggaaaacgaa gacgataagc ttacagggct tgagctgggt gccgacgatt acattataaa
     3721 gccgtttaac agcagagagt tggttagcag ggtccggaac acattgaaac gtattgacag
     3781 aaacaggtgg gcaaatcctc ttaccggttt gcgagggaat attgagatac agactgaaat
     3841 aaaccaaagg attgccacca aacagcactt tgcggttatt tatgcggacc ttgacaattt
     3901 taaggcttac aacgatgtgt atggttttgc cagcggggat agggctatta agctgactgc
     3961 cgatattatc attgataatg tgcataactg cggaaatccc gaagacttta taggtcatat
     4021 tggcggggat gactttatta ttgtctcaac tcccaataag gcagaattgg tatgcaaggg
     4081 aataatcaat gactttgata caaagattaa aagcctttat tgtgatgaag acttgaaaag
     4141 agggtatatt gtgacaagta accggcaggg tcagattatg aagttccctt tgatatccat
     4201 atctttggct attgtatcta acgaaatgag ggatctcatc agtcatattc aaatttctga
     4261 gatagcggca gaactcaaaa agaaggcgaa atccatgccg ggaagcacat atgttaagga
     4321 cagaagaaag agttagaatc attaaatatt ccgtcgtaaa caaaaaacat aatcaaaaga
     4381 tttttcttca tatttaactt aataaggaat ggattcaaac aatttacaag gaggtaagtt
     4441 ttgtgagact taaaagatcc catgcttttt tgttgactgt ttttatatgc ataactttgg
     4501 tgctgcctgg ttgtaaaaat gttgaggccg actataacgg gatttgttta aatcttgggg
     4561 atgagccgtt aacccttgat cctcagttgg tttttgatgt tgttccaatg agggttatta
     4621 atgccgtttt tgaagggtta tgcaggaagg acaaagaagg catgcctgtt cccggtgctg
     4681 cggagcattg ggagataagt gaggacaagc tgacctacac tttttatatc agaaaggatg
     4741 caaagtggta taacggtgcg tctgtcactg caaaggactt taaagatgcc tggatgcgag
     4801 cccttgaccc tgaaccgcaa tatcatgaac ctgcttatat ggcaaacctc tttttttgca
     4861 tagagggagc cgaagagtac gcatatggag aaggcaagcc cgaggatgta gcaattgatg
     4921 cagtggatga caggactctg agggttaccc ttgtacaacc gacaccatac tttttggatc
     4981 tggtatgcaa cagtgtgtat atgccggtta acaaagagtt tttcgatcaa cagatttccg
     5041 gagatatttc agagtatggg acagaagccg aaacgatttt gggtaacggg ccttttatgg
     5101 tagagaaatg gaatcatgat gagagcattg ttttgaaaaa gaataatgca tactggaatt
     5161 cggacaaaat cagcattgaa aagattgagt ttaaaatgat aaaagataat actgctgccg
     5221 ttaactcctt taaggctgga gaaatcgata tggtggaaat aaacgaccac cttctggcag
     5281 atgaattaaa aaacagcaac tgccgggtgg aaacctacga tgcgggaata gttcaataca
     5341 taacaataaa taacgaggat cctgtgctct gcaatgtaaa cataagaaag tccctttcct
     5401 atgctcttga caggaaaacc cttgtggaaa aggtattgaa tgatggttcg aaagaggctt
     5461 ttggctttgt cagccctgtt gtggcagggt atgaaggaaa gttcaggaaa caggcaggag
     5521 acctgtataa ggacaatgat aaagatacgg caagaaagct ccttgaaaaa ggattggatg
     5581 agttaggcct gtctgaaatg ccaaagatca catttttgat taatgaccag gagatatcga
     5641 aaagagatgc ccaggtgttg caggacatgt ggaaaaagag cttggatgtt gatgtagagc
     5701 tgcaggtaat gtcctttgac gcaatgacgg aaaagatgct ttcaaaagat tatcagatgg
     5761 cacttctcat gtggtcggcg gactataatg acccattggc ttatctcgat attttttcgt
     5821 caaacaactt ttataatgtg gcatattacc acaataagca atttgaagag cttttggctg
     5881 aatccatcga ggaaaccgat gtaaagaaaa ggatggattt gcttttgaag gcggaaaaga
     5941 ttgcaataga tgacatggct gtgtgcccgc tttactttgt tgccaacagt tatgcagtta
     6001 aaagccgggt taagggactg gtgagaggaa gcagtgccat acaggatatt gatgtttact
     6061 ggacctatgt agaataggtt aaaataaagg atgcaagcgg ctgctgctat gcacggactg
     6121 cattgaaagg acatactatc cttgagaagg ggatgaaaaa ttgaagtttg tgcggttttt
     6181 gctaaaaaga ttggcattct tttcggtaac catgtggctg gtagtgactt taacctttgt
     6241 acttataagg tgtttgccgg gaagcccgtt taatacggat aaaatgattc atcctcaaat
     6301 acttgaaaat ctcaatacaa agtacggcct tgacaagcct ttttatgaac agtaccttcg
     6361 atatatccaa aaccttgtaa gaggtgatct tggaatatcc atgatttata aaaaccggag
     6421 tgttgcctcc atcataaaaa gagcatttcc ggtctcgctg gatttaggtg tcagggccat
     6481 tatactggca gcgtttgccg gcctttttct gggactgata tcgacacttt acagtaataa
     6541 agcagcggat cacgttattt cagcaataac aatgattgcc gtttcggtgc cgggctttgt
     6601 tgccggagca ctgcttcagt attttttgtg ctatagactg tctgttttaa tacagagcat
     6661 aacccaagtc ggatacagtg tttttcccaa tacggggtgg gagggttttc gttccaccgt
     6721 tgcaccgaca atggcacttg ctgccggagt aatgggaatg gtggtcaaga tgctcagaac
     6781 aagcatgaag gatgtgctgg atcagaatta tatagttatg tccagggcga aggggcttaa
     6841 taagagggaa gttgtgctaa gacacgcatt aaaaaacgca ctgcttcctt tgctgtcgat
     6901 atcgggtcct ttggtttcgt caataattat ggggtctttt gtaatcgaaa acattttttc
     6961 cattccggga ttaggccgat actttgtatc cagcgtgcag gacaaggatt atacgatggt
     7021 tttgggactg gcagttttca gtgtgtttat tgtggttctt ataaacacaa taaccgatat
     7081 attgtacgca gttgtcgacc ccagagtgtg tattgagtca aaagacaggc ttaaaacata
     7141 gtttttccga aatatacagg catgggggca ggggatatgc tcttatatgt aaagaccgct
     7201 gttttcggtg tattaaaaaa taaaatagca ttagccggtg ctgttatttt tttcgttatc
     7261 gttatgatgg caatagcggc accggcgttt tccccctata attactacag ccatgatgca
     7321 gacagcatat ttctaaagcc tatgtcggaa ggacattggt ttggtaccga tgagtttgga
     7381 agggatttgt ttgtgagggt gtggatgggg gcacgaatat ctcttgcaat aggtatcctg
     7441 gcggcgatta ttcagatggt tatcggtact ttatatgggg gaatggcagg cctttttgga
     7501 ggattggcgg acgaggcaat gatgaggctg gcagatgcat tgtattctat tccggacctt
     7561 attttaattg tgcttgttac gctgatgatg ggaaacagcg gcttttcggt ggtactggca
     7621 ctttcattga ccggctggac gtcaacagcc cgggtagtga ggggccaaac ccttatgctc
     7681 aaggaaatgg attttgtgaa ggcggccaag atgttcggtg ccggtagatg gtacatcatg
     7741 ttcaagcaca tacttcccaa ttgcagtgga cagattcttg cgagtttgat gcttaatgta
     7801 ccttcggcta tttttgcaga aagcacatta agttttctcg gtcttggaat aagaccgcct
     7861 gaggcaagct ggggcacttt ggttaatgaa gggtttaagt attcaaacat tacataccat
     7921 ccctgggtcg tgttcttgcc cttgctcttt attgccataa ccatgattag cctgaacata
     7981 ttgggggatg aatttaaaaa ggttctggat tcaaagcatt agtcaataaa ggtggtcttg
     8041 agcatggata caattctaaa gataaaggat ttttccttgt ctattaaagg gaaaaaaata
     8101 ctggataata ttaatatgag cgtttcgaag ggagagatcc ttgccatagt gggtgaatcg
     8161 ggaagtggta aaagcatgct tgcccgatcc atactaagac ttaactcccc ggactttttt
     8221 gacataaaag gagaaatatt ttttcgtgac caggatatta attcactgcc ggaaaaacag
     8281 ttaactaaaa tacggggagc aaaaatagga atggtattcc aagaacccct ttcatatctc
     8341 aatcctgtta ttaaaatggc aagccagctt acggagcctt taaccaaaca taaaaaagtt
     8401 aataaaagaa aagcagccga attaatggaa gacttgctcc ggaagctaaa tataaataat
     8461 cccgagcaat acatgaaaag atatccccac cagttaagcg gaggaatggc acaaaggggc
     8521 gtgatcgcca tgacctcctc ctgtgagcct gagctaatca tagcggatga gcccaccagt
     8581 tctctcgatg taattattca gttttctata acaaagctta taaaggatct tgcaaaactt
     8641 ctgggagtat cggttgtttt cattacccat gatttgcggc tggcagctaa aattgcggac
     8701 agggttgctg taataagcaa aggcagaata gttgaagaag gggatgtgca agaagtattg
     8761 aaaaacccaa agtctgaaga gacggcccaa ttattaaatg cctgtattgt gagtgggggc
     8821 cggcagatcg gtgcaaagag taaagtccta ttggaaatcc
//
LOCUS       DAOOJC010000039         8823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_6938_length_8823_cov_4.264713, whole genome shotgun sequence.
ACCESSION   DAOOJC010000039 DAOOJC010000000
VERSION     DAOOJC010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8823
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6938_length_8823_cov_4.264713"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..114)
                     /locus_tag="PLA01_02005"
     CDS             complement(<1..114)
                     /locus_tag="PLA01_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SpoVG family protein"
                     /protein_id="HPD00106.1"
                     /translation="MEITDVRIRKIDSEGKMKAVVSVTFDNEFVVHDIKVIE"
     gene            complement(333..1151)
                     /gene="purR"
                     /locus_tag="PLA01_02010"
     CDS             complement(333..1151)
                     /gene="purR"
                     /locus_tag="PLA01_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HPD00107.1"
                     /translation="MEKFHRNERIAVLLKTMCDSPGETFTLGSFAEMFGSAKSTVSED
                     IGIVQNLLEKFDLGFIESLPGSSGGVRFVPGYSKNKIRGILNTLCQDLSNSQRILPGG
                     YLYMLDVIYDPRKISEIGYIFAGHFFNREIDCVITVETKGIPLAFSTAKHLGVPLVIA
                     RHNSEATDGPSVNINYVSGSSKKIQTMVLPMRALKRYSKLLFIDDFMKGGGTARGIIE
                     LAREFECEVAGIGVVIETQEPSKKLVEDYVSLLTLNIVNEEEKLIDVKLANDWI"
     gene            1591..3000
                     /gene="murC"
                     /locus_tag="PLA01_02015"
     CDS             1591..3000
                     /gene="murC"
                     /locus_tag="PLA01_02015"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPD00108.1"
                     /translation="MEQLSFLDSKKVKNVHFIGIGGISMSGLAEILLNLGFNISGSDM
                     KSSEITSKLEKKGIKIYIGHSEENINNAELVIYTAAVKGDNPEIVKAKSLGIPIMERA
                     TLLGDIMKKFPFSIAVSGTHGKTTTTSMVSMIMLRAALDPTIHIGGELDAIGGNTRIG
                     GNKYFIAEACEYVESFLKFHPFLAVVLNIEADHLDYFKDIEHIKNAFSKFVSLVPENG
                     YVVACIDDKNVSSLLEKVNCNKITYGINSDNAMWRATDISFNEDGCASFSVLRHNEKI
                     TEIRLKVPGIHNISNSLAAIATCYTLGCNFESIKSGLENFMGTHRRFELKGITDDIKV
                     IDDYAHHPSEIKATLKAARNGNHPRVWSIFQPHTYTRTKLLLNEFAKAFTDADKIIVA
                     DIYSAREIDTGEIHSSILAERIKQNGQDAIYLPSFDKIVEYLKENAVPGDLILTMGAG
                     DIYRVGEMFLKDKQIAAVC"
     gene            complement(3140..3577)
                     /gene="fabZ"
                     /locus_tag="PLA01_02020"
     CDS             complement(3140..3577)
                     /gene="fabZ"
                     /locus_tag="PLA01_02020"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HPD00109.1"
                     /translation="MLTNIEIQNIIPHRYPFLLIDKVVEVEPGKRAVGIKNVSVNEPF
                     FQGHFPGNPIMPGVLIVEALAQTACVAGLMLEENKGKLGVFTGIESMKFRRQVVPGDV
                     LKLEAEFLAFKMGMGKAKVRATVEGEVAAEGEIKFAMIDTNKK"
     gene            complement(3821..4813)
                     /locus_tag="PLA01_02025"
     CDS             complement(3821..4813)
                     /locus_tag="PLA01_02025"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HPD00110.1"
                     /translation="MENRKIKESKNTISNPWIWFFTVCMLFLNAALGFVIILLLVSLI
                     IYSKIYFGGSKFRKIKESITEYITDCNELNEHIEELKSSYIKAKKTDFGEAEFENISR
                     YNYKKKGLNAKFAPNIYDCSRQVCANAQKQPFKYICKYFNINTDEETLSKFEDVLNNF
                     SAAEEGKILLKNKKDEILLSIKTDIPAIIRLFFQKELEKNLGFEEIAFNELYFPRFTF
                     RYISAGGNSGTQFTTTMDIPMLERFVAFIDENVKRRKTAAGQRALMTQRLRNHIKERD
                     RFACRQCGNSIAKEPNLLLEIDHIIPIAKGGLTEESNLQTLCWKCNRSKGSKMM"
     gene            complement(5256..5606)
                     /locus_tag="PLA01_02030"
     CDS             complement(5256..5606)
                     /locus_tag="PLA01_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HPD00111.1"
                     /translation="MNISGIGNESFTNLIKSTEEKVSGDSFEKRLQNAFDNKDEKELM
                     KACKEFEGILLSIVYKQMKATVPKTNLIPSDSGREIFEEMLDEEIAKEASQGSGLGLA
                     DELYKQLSRTLKKG"
     gene            complement(5708..6508)
                     /locus_tag="PLA01_02035"
     CDS             complement(5708..6508)
                     /locus_tag="PLA01_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HPD00112.1"
                     /translation="MMRALWTAGSGMTAQQLNVDVISNNLSNVNTTGYKKARLEFKDM
                     LYDTLNRAYLLDGAGNPVNLQVGHGTAPVATMKNFQTGNLEKTDNPLDFAIDGDGFFT
                     VLGPRGNIVYTRDGSFKISITDNGNMLTTLDGYPVLDESGVEIILDFDVSRLNVSPEG
                     EISYTDENGVVVPLGQRLGIVKFSNRHALESMGSNFYSSNSASGEAVPDLEMGARSNV
                     MQYYLESSNVQVVEEMVKLIVAQRAYEINSKAIQSADEMLGIANNLKR"
     gene            complement(6522..7301)
                     /locus_tag="PLA01_02040"
     CDS             complement(6522..7301)
                     /locus_tag="PLA01_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HPD00113.1"
                     /translation="MIRGLYTSGWSMLANAKQMDVVSNNLANVNTNAYKRDTVVFESF
                     PDAMVRRINDTSSNSNPSGRLGNVQLGNDVGEVFTYYDQGQFMKTDSSLDMAIDNSDS
                     AFFTVAVPSGNGEFRECYTRDGAFALNAARQLVTKDGYFVMGENGLITLQTDDFNILD
                     DGSIIVNGELVDRLLIRNFENPETLRKVGSNLVERTEQTQELEFDGVVRQGYIERSNV
                     NAINEMINMITVMRSYEANQKILQAQDDTLAKAVNEIGVVR"
     gene            complement(7396..8430)
                     /locus_tag="PLA01_02045"
     CDS             complement(7396..8430)
                     /locus_tag="PLA01_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPD00114.1"
                     /translation="MFGLGQDIGIDLGTASIIVYIKGKGIVLGEPSVVAIDKNTNQLF
                     AVGEEARRMLGRTPGNIVAIRPLREGVISDYDITERMLKHFINKVCGNKLFKFFQPRI
                     IVCVPSGVTEVEKRAVREAAMQAGARKTYLIEEPIAAAIGAGVDISKACGSMVIDIGG
                     GTTDIAVISLGGIVVSSSIKVAGDKFDEAITRYMRKKHNIMIGERTAEELKINIGTVY
                     PRVQEVSMDIRGRNLISGLPKTITVTSTEIMEALEEPISSVVEAVHSVLERTPPELAS
                     DISDRGIIMTGGGSLIYGFDKLIQEKTGINVIVADDAISCVALGTGKALEHIEIIEKS
                     DPNDKKASYR"
     gene            complement(8564..>8823)
                     /locus_tag="PLA01_02050"
     CDS             complement(8564..>8823)
                     /locus_tag="PLA01_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00115.1"
                     /translation="LADIKRMKQTLYWLVVPRKIDCMSDETLFNRDDSVRRLKIDSER
                     VGYYKVFKVNGILEDYIIVDESVVSILSLGNFSVLNLKYYN"
BASE COUNT         2608 a         1939 c         1395 g         2881 t
ORIGIN      
        1 ctcaataacc tttatgtcat gaacaacaaa ttcattatca aaggtcactg aaacaacagc
       61 cttcatttta ccttcagaat caattttcct gatacgaaca tcggtaattt ccatgagatg
      121 ctaccacctt ccgtaaattg tgtattcatc gtgtactatt attatattcg tcattatttt
      181 cgaatttcct tcttattttc tcaccatttt aatttttatt tttcgttaaa tataacctct
      241 taatagcatt tctcattata agcatattat tcataacaaa tacatattgt ctaaaaatat
      301 atctttatca gccaacaaca atgaagaaaa ggtcaaatcc aatcgtttgc cagctttaca
      361 tcaataagct tttcttcttc attaacgata ttcaaggtta atagagagac ataatcctca
      421 accaactttt ttgaaggctc ctgagtttcg atgacaaccc cgataccggc gacctcgcat
      481 tcaaactctc ttgcaagctc aataattcct ctggccgtgc ctccgccttt cataaaatca
      541 tctataaaaa gcagcttcga ataccgtttc aaagctctca tgggaagcac catggtttgt
      601 attttcttcg aagacccgga tacataattt atgtttactg atggtccgtc ggtggcttca
      661 ctgttatgcc ttgctataac caggggtacg cctaagtgct ttgccgttga gaaagcaagg
      721 ggtattccct tggtctcgac tgtgataaca caatcaattt ccctattaaa gaagtgtccg
      781 gcaaaaatat aacctatttc ggatatttta cgcgggtcat aaataacatc gagcatatac
      841 aaatatcctc cgggaagaat cctttggctg ttggataaat cttggcacaa agtatttaga
      901 atacctctta ttttattctt gctatagccc ggtacaaacc ttactccccc tgaggaaccc
      961 ggcaaacttt ctataaaacc caaatcgaat ttctcaagca gattttgaac aatacctata
     1021 tcctcactta ccgtagactt cgcagacccg aacatctctg caaagctccc cagtgtaaat
     1081 gtttctcctg gtgaatcgca catggtttta agcaatactg caattctctc atttctatga
     1141 aacttttcca ttatatcccc ccgggcctgt tttatacttt ttccatcctg ttggatgcaa
     1201 acatgtataa aataagcaat cttcttatta aaattcactc tatataaaaa tataatatta
     1261 ttatactata tatatccgaa tattttaagc attattagtc aaattgttca gcaattatgt
     1321 gtcccgattg caatctttat aacgcttcgg ttcaaagata gtttttcgct tgcataaaaa
     1381 ctttctttca ttacattttt tattttaaat tatctaatta attatgctga acacttataa
     1441 ttgtgctaaa atttacatat aatattaatt ggatttttgt ttttatgtat tgcattaaat
     1501 tcagaaagga aataaatttc ataattattt gaaaaatgta gaaaatgttc ttcgacagac
     1561 atatagtata taatttaagg agtgtttata ttggaacagc taagtttttt ggattcaaaa
     1621 aaagtcaaaa acgtacattt tataggtatt ggcggaataa gtatgagcgg cctggcagaa
     1681 atcctgttaa acttggggtt taacatatcc ggttctgaca tgaagtcttc cgaaataact
     1741 tcgaagcttg aaaaaaaagg aattaaaata tatatcggtc acagcgaaga aaatataaat
     1801 aatgctgaac ttgtaatata cacagcagct gtcaagggag acaatcctga gattgtcaaa
     1861 gccaaaagcc ttggaatacc gatcatggaa agggcaaccc tgttgggtga cattatgaaa
     1921 aagttccctt tcagcatcgc tgtttcgggt actcatggaa aaacaactac gacatcaatg
     1981 gtttcaatga ttatgctaag ggctgccctc gacccaacca tacatatcgg aggtgaactg
     2041 gatgcaatag gaggcaacac aagaatcggc ggcaataaat actttattgc cgaagcatgc
     2101 gaatatgtgg aaagctttct aaagttccac ccgtttctgg ctgttgtcct gaatatagag
     2161 gcagaccacc tcgactactt taaagatata gagcatatta aaaatgcgtt ttcaaagttt
     2221 gtatccctgg tcccagaaaa cgggtacgtt gtggcatgca tagacgataa aaacgtttct
     2281 tccctgctcg aaaaagtgaa ttgcaacaaa attacctatg gcatcaattc cgataatgcc
     2341 atgtggaggg caacggacat cagctttaat gaagacggct gtgcttcctt cagtgtgcta
     2401 aggcataatg aaaaaattac ggagataagg ctcaaggttc cgggaattca caacataagc
     2461 aactcactgg ctgcaattgc gacatgttat accctgggct gcaattttga gagtataaaa
     2521 agcggtttgg aaaactttat gggaactcac aggcgttttg aattaaaggg aattacagat
     2581 gacatcaagg tcatcgatga ttatgcccat catccttcag agatcaaagc gacattaaaa
     2641 gccgcccgca atggaaatca tccaagagta tggtcgatat ttcagcccca tacatacacc
     2701 cgtacaaaac ttcttttaaa tgagtttgcc aaggcattca ccgatgcaga taaaatcatt
     2761 gttgcagata tctattctgc ccgagaaatc gatacgggag agatacattc cagtatcctt
     2821 gccgaaagga taaagcaaaa cggacaagat gccatatatt tgcccagttt tgataaaatc
     2881 gttgaatacc ttaaagaaaa tgcagtcccc ggtgacttga tactcaccat gggtgccggt
     2941 gacatataca gagttggaga aatgtttctt aaagacaaac aaatagccgc agtctgttaa
     3001 gagaagcaaa cacaagtaaa gaaaaattag gtcatttcac ttcgtgaaat cccactaatt
     3061 aaaaagagat ggcagatttt atgtacgaat cctccatctc ttaatttatg cttataatta
     3121 taacgaattt atagctctgt tattttttat tcgtatcaat cattgcaaat tttatttctc
     3181 cttcggctgc aacctcgcct tcaacggtag cacggacctt ggcctttccc atacccattt
     3241 tgaaagccag aaactccgct tccagcttta gtacatcacc gggaaccacc tgtcttctga
     3301 acttcataga ctcaataccc gtaaaaactc caagcttccc tttgttctct tcaagcatca
     3361 accctgcaac gcaagcagtc tgtgccagag cctctacaat gagaactccg ggcataatgg
     3421 gattgccggg aaaatggccc tgaaaaaatg gctcatttac tgaaacattc ttaattccca
     3481 ccgctctctt acccggttcg acttcaacta ccttgtcaat cagtaaaaaa ggatacctgt
     3541 ggggaataat attctgaatt tctatatttg tcagcattca aacacctctt tccaaaatta
     3601 ttcaatcggc atatattata gcacagagtt gttgttttgt aaaaataaat gctgtctaac
     3661 ttaatgtctc atcccccata aagtatttta gcattactgc cgagagtatt tcccagtttt
     3721 tcattatagg tttgaagttt ctgacttttt taaaaaatct aaagttctga gttttttata
     3781 gtgtcaatag aacgctcgaa cgcagcgaga atttttccga ttacatcatt ttagaacctt
     3841 tcgaacgatt gcatttccag caaagcgtct gtaaattgct ttcttctgta agtccgcctt
     3901 tggcaatcgg aataatatgg tcaatttcca atagtagatt aggctccttt gctatcgaat
     3961 taccgcattg cctgcatgca aatctgtcac gctctttaat atggtttctt agtctttgtg
     4021 tcattaatgc tctttgaccg gcagcagttt ttcgtctctt gacattttca tcgataaaag
     4081 caacaaatct ttcaagcatt ggtatatcca ttgttgtagt aaattgtgtg cctgaatttc
     4141 cgccggctga aatgtatctg aatgtaaacc gcggaaagta taactcatta aaagcaattt
     4201 cttcaaagcc taaattcttt tccaactcct tttgaaaaaa taatctgata attgccggaa
     4261 tatctgtttt gatgcttaaa aggatttcat cttttttatt tttcaataaa atcttacctt
     4321 cttcagcagc ggaaaaatta tttaaaacat cttcaaattt tgataatgtt tcttcgtcag
     4381 tatttatgtt gaaatatttg caaatatatt tgaatggttg cttttgagca ttcgcacata
     4441 cttgtctgct acaatcgtaa atattaggtg caaattttgc attcagtcct ttctttttgt
     4501 aattatacct gctaatattt tcaaattcgg cttctccaaa atctgttttc tttgctttaa
     4561 tgtaagaact tttaagttct tcgatatgtt catttaactc attacagtct gtaatatatt
     4621 cggtgatact ttccttgatt tttctaaatt tactgcctcc gaaataaatt tttgaataaa
     4681 ttattaagct taccaacagc aaaataataa caaatcctaa cgcagcattc aaaaacaaca
     4741 tacaaactgt aaaaaaccaa atccacggat ttgatatggt atttttgctt tctttaatct
     4801 tccggttttc catttaaaat cgatgcctcc tattatcagt agctatatta tttatttagt
     4861 tgcattattt ttaaagcaac taattattta tatcctcacg cattttctct ttgtattttg
     4921 caatctattc ctttgatttt tctcttttac atcataattt attaatacaa tcaatatgat
     4981 ttacaaagat tttttataaa attcaaaatt atcgggcata tttagagaaa ttcagaaagt
     5041 tttccttttc gtccaataaa atatgctcct cctcacagta aacagtttta tactatcacc
     5101 gtttacttat gaggggagca tatcaattct tatacaatcc ctaattactt ttttaagaaa
     5161 acttccaacc gctatttggt ttatagctgc aagacaccaa ttacaatatg tgccggcact
     5221 ttaaaccggc gtttcattat tgccccgtcc tgcaatcaac cctttttaag ggtcctgcta
     5281 agctgcttat aaagttcatc cgccagcccc aaacctgaac cttgggatgc ttcctttgct
     5341 atttcttcat caagcatctc ttcaaaaatc tctcttcctg aatcggaagg tattaaattt
     5401 gtttttggta ctgtggcttt catctgttta tacacaatac taagaagtat tccttcaaac
     5461 tccttgcacg ccttcataag ctccttctca tctttgttgt caaaggcgtt ttgcagcctc
     5521 ttctcaaaac tgtccccgga aaccttctct tccgtgcttt ttattaaatt agtaaaagat
     5581 tcattgccta ttccgctgat attcatattg acgcctccgt cttttattcc cggtcaatat
     5641 tgcttccaca cccggcactg cttattggat tgcaactata attgccgtat gctgtctgaa
     5701 attgccgcta tctcttgagg ttgtttgcaa taccgagcat ttcatcggca gattgaattg
     5761 cctttgaatt gatttcataa gccctttgtg caacaataag cttaaccatt tcctcaacca
     5821 cttgcacatt ggatgattca aggtaatact gcattacatt gctcctggca cccatttcaa
     5881 gatcgggcac tgcctctccc gatgcagaat tgcttgaata gaaattgctg cccatgcttt
     5941 ccagtgcatg cctgtttgaa aacttaacaa tacccagcct ctgtcctaaa ggtactacaa
     6001 ctccattttc atcggtataa ctaatttctc cttccggtga aacattgagc ctggatacat
     6061 caaagtcaag aattatttcc actccggact catctaaaac cggatatccg tccaatgttg
     6121 taagcatgtt gccattatcc gttatactga ttttaaagct gccgtcccgg gtatatacaa
     6181 tattgcccct tggtcccaat acagtaaaga atccgtcgcc gtctatggca aaatccaggg
     6241 gattgtccgt cttttcaagg ttcccggtct ggaagttctt catcgtagcc accggagctg
     6301 tcccgtgtcc tacttgaaga ttcaccggat tgcctgctcc atcaagaaga taagccctgt
     6361 tcaatgtatc ataaagcatg tccttaaact ctaatcttgc ttttttatat cctgtagtgt
     6421 taacatttga aagattgttt gatattacat caacattcaa ctgctgtgcc gtcattccgg
     6481 atccggctgt ccacaatgct ctcatcatag cttataccct cctatctcac cactccaatt
     6541 tcattcactg cctttgccaa ggtatcatcc tgtgcttgaa gaattttttg atttgcttcg
     6601 tatgaacgca ttactgtaat catgtttatc atttcattta ttgcattgac atttgaccgc
     6661 tcaatatatc cctgtcttac aacgccgtca aattccaact cctgtgtttg ctctgtcctc
     6721 tccacaagat tagagcctac ttttctcagc gtctcaggat tttcaaaatt tcttatgaga
     6781 agcctgtcaa ccagttcacc gttcacaata attgaaccat catcaagaat attaaaatca
     6841 tctgtctgaa gtgtaataag accgttttct cccatgacaa aataaccgtc cttggttacc
     6901 agttgtctgg cggcattcag ggcaaaggca ccatctcttg tatagcattc cctgaattcc
     6961 ccgttaccgg aaggcactgc cacggtaaaa aacgccgaat ctgaattgtc aatagccatg
     7021 tcaaggctac tgtctgtctt catgaactgt ccctgatcat agtacgtgaa aacctcaccc
     7081 acatcatttc ccaactgcac attcccaagc cttccagagg gattggaatt gcttgatgtg
     7141 tcgttaatac gccttaccat cgcatccgga aaactttcaa atacaacggt atcccttttg
     7201 taagcattgg tattaacatt ggcaaggtta tttgatacaa catccatctg ttttgcgttg
     7261 gccagcatac tccagccgga cgtgtaaaga cctctaatca tagaatatct cccgatatat
     7321 gttttctctt acataaaaca atatcggaaa tataataaaa taactttaca gaaacataaa
     7381 ataaaacata ttttcttatc tataacttgc ttttttgtcg ttgggatcgc ttttttcgat
     7441 tatttctata tgttcgaggg cttttccggt acctaaagcc acacatgata tggcatcgtc
     7501 cgcaacaata acgttaatgc ccgtcttttc ctgtataagc ttgtcaaacc cgtatataag
     7561 acttcctcca cccgtcatta tgattcccct gtcactaata tcagaagcaa gctccggcgg
     7621 cgtcctttca agtacggaat gcactgcctc aacaacactg gaaataggct cttccaaagc
     7681 ctccataatc tccgttgagg taacggttat ggttttgggc agtccggaaa taagatttct
     7741 tcctctaata tccatggata cttcctgtac tctggggtaa accgttccga tatttatttt
     7801 taattcctcg gcagtacgtt cacctatcat aatgttgtgc ttttttctca tgtatctggt
     7861 aatagcttca tcaaatttgt ccccggcgac tttaattgaa gaactcacta caattccgcc
     7921 taaggatatg acagcaatgt cggtggtgcc cccgccaata tcaattacca tgcttccaca
     7981 tgccttggat atatcaacac cggcaccgat tgcagccgct ataggctcct caataagata
     8041 tgtctttctc gcaccggcct gcattgctgc ttcccttaca gctcttttct caacctcggt
     8101 aaccccgctg ggtacacata caataattct cggctggaag aacttaaaca atttattgcc
     8161 acaaacttta ttaataaaat gctttaacat tctttccgtt atatcgtagt cagaaataac
     8221 gccttctcga agcggtctta ttgcgacaat atttcccgga gttctcccaa gcattcgcct
     8281 ggcttcttct ccaacagcaa aaagctgatt cgtattttta tcaatcgcta caaccgaagg
     8341 ctctccaaga acaatacctt tcccctttat ataaacgatt attgatgccg ttcccaaatc
     8401 tattcctata tcctgtccaa gcccgaacaa agtgcatctc cccttaaatc tgtattggaa
     8461 atgtagataa aatatattca taataatatt atcacattat actattattg caagaatttt
     8521 ttattatata ttataggtag ggaacagtta ttgaagggaa gaattaatta tagtatttca
     8581 aatttaaaac cgaaaagtta cccaagctta gaatactaac aacagactca tctacaataa
     8641 tatagtcctc taatattccg ttgaccttaa aaactttata atacccgact ctttcgctgt
     8701 caatctttaa ccttcgaaca ctgtcgtcac gattaaacaa tgtttcatca gacatgcaat
     8761 ctattttccg gggaacaacc aaccaataca gtgtctgttt cattctttta atatccgcaa
     8821 gca
//
LOCUS       DAOOJC010000040         8733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_7019_length_8733_cov_3.220212, whole genome shotgun sequence.
ACCESSION   DAOOJC010000040 DAOOJC010000000
VERSION     DAOOJC010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 8733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8733
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7019_length_8733_cov_3.220212"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            441..2165
                     /locus_tag="PLA01_02055"
     CDS             441..2165
                     /locus_tag="PLA01_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPD00116.1"
                     /translation="MDADERVNEEKSLDEDKKMYSNFIQDFIDEDNESNRFGRRVHTR
                     FPPEPNGYLHIGHAKSICLNFGIAAQNRGLCNLRFDDTNPVKEDTEYVDSIKEDIKWL
                     GFDWEDRLYYASDYFERLYDCAVKLIEMGKAYVCDLTADEIRDYRGTLTEPGQESPYR
                     NRSIEENLDLFRRMRDGEFEDGSKVLRAKIDMASPNLNMRDPVIYRIVKAEHHRTGNK
                     WCIYPMYDFAHPLSDSFEGITHSICTLEFEDHRPLYNWFLETLDMECKTRQIEFARLN
                     LTYTVMSKRKLLKLVKEGYVRGWDDPRMPTISGLRRRGYTPESIRNFCSRIGVAKSNS
                     MVDIALLEHCIREELNQKATRVMAVLRPLKVIIDNYPEDSVEYFEVPNNPEDPDSGTR
                     TVPFSRVIYIEQDDFCENPPKKYFRLAPGQEVRLKSTYFIKCVDIVKDDATEEIIELH
                     CTYDPESRGGNSPDGRKVKGTIHWVSAAHAVDAEVRLYNNLFDDPNPGADEGVDFIDQ
                     LNPDSLEVITQCKLEPGLKDAKPGDMFQFLRLGYFCVDVVDSKEDALVFNRTVSLKDT
                     WAKISRKE"
     gene            complement(2259..3143)
                     /locus_tag="PLA01_02060"
     CDS             complement(2259..3143)
                     /locus_tag="PLA01_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00117.1"
                     /translation="MDKTSNKHIELKSDRLSIEIAYPGTVYSGSRFDWNGFITGIVLD
                     KKHSFCVPESPIPGQGSGGFGLCNEFGIETPIGYDAAKPGEKFLKLGTGLLTRPDESD
                     YFFFDKYEVTPFPVKIETGYNTAVFRVEPVDCNGYAVKMTKKIFVDNNKLFIDYNLEN
                     MGIKPIITEEYCHNFFAVNNNKIGTGYLLKFPYNINPDCVPEVMDIKDRGIRWNTVPK
                     KDFKCCPKGFQNVTPHYWELLYEPDRVGIREFSEFPVANVAVWGTTHVVSPEVFIKVN
                     IKPGETQKWTRKYEFFTY"
     gene            complement(3247..4449)
                     /locus_tag="PLA01_02065"
     CDS             complement(3247..4449)
                     /locus_tag="PLA01_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00118.1"
                     /translation="MTKKTFISIVLFILLINTYAYALDYSFSARINNSGNQKYKAVKL
                     TPQIYNNTVENLADLEIFDDNNQPVPYFINSYYESQISENNIYEMELINSFVNDVDHY
                     FDYAVKSLDTKELFATSIEVEPDRNVFAVRLVVYGSHSNKNWEKIQEDVLYNVDGNKK
                     LEIVFKWTKEYTHYRFKIYNNIEKVSFSSVKLKYNKVLHKKEYFTETFMPDFSIEEKE
                     NSTVIKVRGLKNLKLCSLSLITPSMFKRNVTFDGRTSKMLYNLSFENVEYKDLTLPIE
                     PYKVTSDTSEIVIDNKDDKPIKVSAIVATYLADELVFESSGSAVYTLKFGNSDIYNPK
                     SYDLSNYKEYILSEGYDMLSIEDVKGSPAKDPLSTKEPYDYKLIFNIAISIVAIVLGV
                     IIFLKIKK"
     gene            complement(4427..6967)
                     /locus_tag="PLA01_02070"
     CDS             complement(4427..6967)
                     /locus_tag="PLA01_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HPD00119.1"
                     /translation="MSMVQNLKAVLDKQKDILASLEKEIASIEATDLISENQKLKTEL
                     TKYHESISELKSDNTRLTEENKRLRTALYEQFYNEKIQVLNAAEKRMDVYYRANFEGE
                     INRLTKLEMFIKTKINEMERVLRENRINLEDEIFVRLEELRNQLNVKVAKAREEIMKQ
                     TKAYSEIKAAELSKLRQEQITEAEIRARTKQNNIESLIGLNVINKLGILLLVIGVITA
                     AQFTYFKLPDTLKGLFTFAVGILLLVAGELLNRKKPNVFSLGITSGGVAILYVGLVLS
                     YFQFNILGMYQALGLCVLITAGAFVLSQRYNSQTISAFAMIGGYLPIFSIAGSKTMVY
                     GAMVYFIVLNLLALIISVNKKWTVTAYIGFVLNVIGTVYIGNILISGTPFRPQFTSDG
                     LISVFYILFAFINYTLIPITGTYRKKIGFKNSDIVLLTLNTLISSFLLYMALYSVDLK
                     DFAGFLSLVLSIVYLSLGWFIEKKMRKEKKVAVLFYITGLTSVILIIPFQFGKVWLTL
                     GWLVEGIAILSYGIYKELKGFKKAGTVISLLCLWSFLFFDIINYHDSLFTFKYLAITA
                     GSFIVLGTMIYKKHLFNIPSQVFKYCTFINLWLFSLYLIVRELKPLISEMMQKSHYNS
                     DFLIFAAMILVSFVIAYVIPRIKILCDDVMKGISVSIYTLSFLSVFALNFVSPVKGYL
                     TQVPMAVKVVGTIELAVIAVLAILAMRDLLLHFMIGRELGIEWYPLVLSFYFILILTQ
                     NLIIQYSLEFNNAVISIIYIATALAWITFGFIKRYVLIRRFGLALSMLSVAKLFIIDL
                     AFLTQGYRIVSYFVFGLILIAISFVYQYFSKRIENISEVMPDDKKDIH"
     gene            7192..>8733
                     /locus_tag="PLA01_02075"
     CDS             7192..>8733
                     /locus_tag="PLA01_02075"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HPD00120.1"
                     /translation="MIGKKSFSIILVIALVLGFINPVYARQDEITVNFNGAITMFLDG
                     EEFVPVETDRTYLTPVIYNGRTYLPVRALCDALKLYVDWDASTKTVSIGGGEWIPPQK
                     VTPIGSKDKSLRTGKAYRDTDLKMVLYGRPFNPREQDGTLVSPILVNNRTYLPLRALC
                     DAVGVDVQWDGATSTITMTRNGKGPGKDGEYIHDGSTYEENISYYQDLEPIYNVPVDQ
                     VFKFKLKLSDKPFASFGIKNMFKVFSDRDCTTELIANVEYDEESNTISIKPDSSSWGM
                     YPREGVDVKGLSWGGLQKYYIVIYHDLNATEVTELSKPMRLMFTVGSDIPIPTVKVKK
                     DNNGNIVLTWNKIEGAQKYKIYRGSRYGMELLRETTDTSMSMTEEEYVDVAMNTLLSD
                     VYKYAVTSVVNGKESRLSNIISGEDYISIAPGKMTTLSKYISVGDGFDVDDIFSLPKS
                     VSVDYLEYIPNDVPGRYGSYKTSNHKVIWNFSKNVGDANYPRYEGKVMGTTFTLFATF
                     FDNPPT"
BASE COUNT         2707 a         1550 c         1791 g         2685 t
ORIGIN      
        1 tacggttcaa cgagagggaa ataattctta gcaattattt ctctactcta ttcacttctt
       61 aaaatcccac gaatgaaagt gaaagttcat cgagccctca tcgctattta tatagtttga
      121 cacgtccgtt gattaaggat ataatatttt atgattttaa tgagcagtcc tgtacattga
      181 gaaaaagtag taacggatag tattatagag gctttaaaag cgagcttctt gaaaaagaat
      241 gcttgaggta tgggcatttg ctgaagtatt gaggaaaatg tgtttttttg cttataatat
      301 ttaaatgaaa tttgaaatac tattctataa tgcttagaag ttgaatgatt tgaattatgt
      361 tatataataa tatattatgt gcatttgatt gacataggat ttgtcagagt tcttttaata
      421 agatggagga ggaaaaaaga atggatgctg atgaaagagt aaatgaagag aaatccctgg
      481 atgaggacaa aaaaatgtat tccaatttta ttcaggattt tatagatgaa gacaatgaaa
      541 gcaataggtt tggcagaaga gtacatacga gatttccgcc cgaacccaac ggatatcttc
      601 atatagggca tgcaaaatca atttgtctga attttggaat tgcagctcaa aacagaggtt
      661 tatgtaattt gaggtttgat gacacaaatc ctgttaagga agataccgaa tatgtagatt
      721 ctataaaaga ggatattaaa tggttgggat tcgattggga agacaggctg tattacgcat
      781 ctgattattt cgaaagatta tatgactgtg cagtgaagct tattgagatg ggaaaagctt
      841 atgtatgtga tttaactgct gatgaaatac gcgattatag aggcactctc acagaaccgg
      901 gtcaggaaag cccttaccgc aacaggagca ttgaagaaaa cctcgatttg ttcagaagaa
      961 tgagagacgg tgagtttgag gacggttcaa aggtgctgag agcaaaaatc gatatggctt
     1021 cacccaactt gaatatgcgt gatccggtta tttatagaat agttaaggca gagcaccaca
     1081 gaacgggaaa caaatggtgt atttatccta tgtacgattt tgcccacccc ctttccgact
     1141 cctttgaggg gataacccat tctatctgta cactggaatt cgaagaccat aggccattgt
     1201 acaattggtt tttagagacc cttgacatgg agtgtaagac gaggcagata gagtttgccc
     1261 gtttgaacct aacctacact gttatgagta aaagaaagct tcttaagctt gtaaaggaag
     1321 ggtacgtaag aggatgggac gatccaagaa tgcctacaat atcaggcctc agaagacggg
     1381 gatatactcc ggaatctatt aggaatttct gttcccggat aggggttgcg aaaagtaaca
     1441 gtatggtgga tattgccctc cttgagcatt gtatcaggga agagcttaat cagaaggcta
     1501 ccagggtaat ggctgtattg agacctctta aggttattat agacaactat ccggaagata
     1561 gtgttgagta ttttgaggtg ccgaacaatc ctgaagaccc tgattccgga acaaggacag
     1621 ttccgttttc aagagtaatt tatatagaac aggatgattt ctgcgaaaat ccgccgaaga
     1681 agtatttccg actggcaccc gggcaggagg tcaggcttaa gagcacttac tttattaaat
     1741 gcgtggatat tgtaaaggat gatgcaactg aagagataat tgagctgcat tgcacctatg
     1801 acccggagtc aagaggtgga aattctcctg acggacgcaa ggtcaaaggt actattcact
     1861 gggtatctgc agctcatgct gttgatgctg aggtacggct gtacaacaat ctgtttgacg
     1921 atcccaatcc cggtgctgac gagggtgttg actttattga ccagttaaac cccgattctt
     1981 tggaggttat tactcaatgt aagctggaac cggggcttaa agatgcaaaa cccggtgaca
     2041 tgttccagtt cttgagattg ggttacttct gtgttgatgt tgtggattcc aaggaagacg
     2101 cattggtatt taacaggacg gtatccctga aggacacatg ggcaaagata tcaagaaaag
     2161 aatagcatat tatataaata aaacaagctc agggatttgg tctctgagct tgtttgcggt
     2221 atcaccggtt tttattatgt tcatatttgt tctctcaatc aatatgtaaa aaactcatat
     2281 ttccttgtcc atttctgggt ctcgcccggc tttatattga ctttgataaa cacttccgga
     2341 cttacgacat gggttgtgcc ccagacagct acgtttgcga caggaaactc actgaattct
     2401 ctaataccga ctctatccgg ctcatataaa agttcccaat aatgcggcgt aacattttga
     2461 aatcctttgg gacaacattt aaaatctttt ttaggtacgg tgttccatcg aattccacgg
     2521 tcttttatat ccataacctc aggaacacaa tccggattga tgttataagg aaatttcaat
     2581 aagtacccgg taccaatctt gttgttgttt actgcaaaga aattgtggca gtattcctca
     2641 gttataatgg gtttgatgcc catattttca aggttatagt caatgaacaa tttgttattg
     2701 tcaacaaaaa tctttttggt cattttgacg gcgtaaccgt tgcagtctac aggttcaacc
     2761 ctgaataccg cagtgttgta acctgtttca attttaacgg gaaaaggagt aacctcgtat
     2821 ttgtcaaaga aaaagtagtc tgattcatcg ggtcgggtta aaagacctgt gccgagcttt
     2881 aaaaactttt caccgggctt tgccgcatca tatcctatgg gtgtttcgat accgaattca
     2941 ttgcaaagac cgaaaccgcc ggatccctga ccggggatag gtgactcagg cacacaaaag
     3001 gagtgctttt tatctaaaac tattcctgta ataaagccgt tccaatcaaa acgggagccg
     3061 ctgtataccg ttcccggata tgctatttca atgctaagcc tgtcactttt taattctata
     3121 tgtttatttg atgtcttgtc catgagcaaa cctcctgtta ataattatac agtcaatatt
     3181 ttaccacaaa gttttagatt tagaagcttt ttttcactgc tttagcaaca ggcgtggatg
     3241 taaattttat ttttttatct tcagaaatat aattacaccg aggactatag caactattga
     3301 tatcgcaatg ttaaaaatca gtttgtagtc gtagggctct ttagtggata aagggtcctt
     3361 ggcaggactg cctttaacat cttctatact taacatatca tagccttcgc ttaaaatgta
     3421 ttctttgtaa ttggacaggt cataactttt gggattataa atatcgctgt tcccgaattt
     3481 caatgtatac acagcggaac ctgaactttc aaacaccaat tcgtcagcca gataagtggc
     3541 tacgattgcc gatactttaa tgggtttgtc atctttgttg tcaattacaa tttcactggt
     3601 atcggaggtt actttgtacg gttctatggg aagggtcaaa tccttatact ccacgttttc
     3661 aaagctcaga ttatacagca ttttggatgt tctgccgtca aaggtaacat tcctcttaaa
     3721 catgctgggg gtaataaggg aaaggctgca cagcttcagg tttttaagcc cccggacttt
     3781 tataactgtt gaattttctt tttcctcaat gctgaaatcc ggcataaaag tctctgtaaa
     3841 atactctttt ttatgcagca ctttattgta ttttagctta accgaagaaa aagagacctt
     3901 ttctatgttg ttatagattt taaaacggta atgtgtgtat tcttttgtcc atttaaagac
     3961 tatttcgagt tttttattgc catcaacatt gtaaaggaca tcttcctgga tcttttccca
     4021 gtttttgttg ctatggcttc cgtatactac aagcctcact gcgaaaacat tcctgtcggg
     4081 ttcaacctcg atggatgtgg caaaaagttc tttggtgtca agacttttga cagcataatc
     4141 gaaataatga tccacgtcat tgacaaaaga gtttattagc tccatttcat atatattgtt
     4201 ttctgaaatc tggctttcat aatagctgtt tataaaatag ggcaccggct ggttattatc
     4261 atcgaagatc tccaaatctg cgaggttttc aacggtatta ttgtatattt gcggtgtgag
     4321 ctttactgcc ttgtatttct ggtttccgct attattaatc cgggcactga aggaatagtc
     4381 cagagcatat gcataagtat taattaataa gatgaatagc actatactaa tgaatgtctt
     4441 ttttgtcatc gggcattacc tcgcttatat tttctattct tttactgaaa tactgatata
     4501 caaaggatat ggctatcaga attaaaccga aaacaaagta tgacactatt ctgtaccctt
     4561 gggtcagaaa cgcgagatca ataatgaaga gctttgccac cgacagcatt gataatgcaa
     4621 gaccgaaccg ccttatgagc acataccgct ttataaagcc aaaggtaatc cacgcaaggg
     4681 ctgttgcaat gtaaatgatg cttattacag cgttgttgaa ttcgaggctg tactgtatta
     4741 taaggttttg agtaaggatt aatatgaaat agaaggataa aaccagtgga taccattcta
     4801 tacccagttc ccgtcctatc ataaaatgca gcagcaaatc cctcatggca agaatcgcta
     4861 aaactgcaat aactgccaat tctatagttc ctacgacttt tacagccata ggaacttgtg
     4921 taaggtatcc ttttacgggc gatacgaaat tcaaagcaaa gacagaaaga aatgaaaggg
     4981 tatagattga cacggatatg cctttcatga catcatcaca tagaattttt attcttggaa
     5041 tgacataggc aataacaaaa cttacaagaa tcatggctgc aaaaataagg aaatctgagt
     5101 tatagtggct tttttgcatc atttccgata tgagcggttt taactccctc actatgaggt
     5161 aaagagagaa aagccataaa ttgatgaagg tacaatactt gaaaacctga gacggtatgt
     5221 tgaatagatg ctttttatat atcattgtac ctaaaacaat gaagcttcct gcggttatcg
     5281 caagatactt gaaggtaaac agtgagtcgt gatagttgat tatatcaaag aacaagaagg
     5341 accaaaggca gaggagtgaa atcacggttc ctgccttttt aaagcctttt aattccttgt
     5401 atattccata ggaaagaatt gcaattccct caaccagcca tcccaatgtc agccatacct
     5461 tgccgaattg gaagggtata attaatatga cagaggtaag tcctgtgata taaaacaaga
     5521 ctgctacctt tttttctttt ctcatctttt tttcaataaa ccaacccaag gacaggtata
     5581 cgattgacaa gaccagggaa agaaaaccgg caaagtcttt cagatccaca gaataaagtg
     5641 ccatatataa aaggaaactg cttattaagg tgtttaatgt aagcagtact atgtcagagt
     5701 ttttaaatcc tatttttttc ctgtaagtac cggtaatagg aatcagtgta tagtttatga
     5761 aagcaaataa aatataaaat accgatatca acccatcact tgtaaactga ggtctaaagg
     5821 gggttccaga tataagtatg ttccctatat agacagtgcc tatgacattt aagacaaacc
     5881 cgatatatgc tgttaccgtc cactttttat taacggatat tatcagtgca agaaggttca
     5941 gaacaataaa gtataccatt gcaccgtata ccattgtttt gcttcccgct atagaaaaaa
     6001 tgggaaggta tccgccgatc atggcaaagg cagaaatggt ctgggagttg taccgctggg
     6061 ataaaacaaa ggctccagca gttattaata cacataatcc cagagcttga tacatgccca
     6121 gaatgttgaa ttggaaataa ctaagcacca atcccacata caatatggcg acgcctccgc
     6181 tagtgatgcc cagagaaaaa acattgggct ttttacggtt taacagctct cccgctacaa
     6241 gcagcaagat tcccactgca aaagtaaaga gacccttaag ggtatcggga agcttgaagt
     6301 aagtaaactg tgctgcagtg attacaccga taacaagaag cagaatgccc agcttgttaa
     6361 ttacattaag tccgataagg gattcaatgt tgttttgctt tgtccgggct ctaatctctg
     6421 cctctgtaat ctgttcctgc cttagcttcg atagttcggc ggctttgatt tcgctgtagg
     6481 ctttcgtttg cttcataatt tcttcccggg cttttgccac ctttacattc aactggtttc
     6541 tcaattcttc gaggcgtaca aatatctcat cctcaagatt aatcctgttt tctctcaata
     6601 ctctttccat ttcattaatt ttggtcttaa tgaacatttc aagctttgtt aacctgttta
     6661 tttctccttc aaaattagcc ctgtagtaaa cgtccattct cttttccgct gcattgagaa
     6721 cctggatttt ttcgttgtaa aactgttcat acagtgctgt cctaagtctt ttattctctt
     6781 cggttaacct tgtattgtcg gattttaact cacttatgga ttcgtggtat ttggtaagtt
     6841 ctgttttcag tttctgattt tctgagatca aatcagttgc ttcaatactg gcaatttctt
     6901 tttcgagact tgccagaata tctttttgct tatcaaggac agccttaagg ttttgtacca
     6961 ttgacatagg aattcctcca gacgacaaaa atgtgtacag ccagaaaaca agcatttata
     7021 atgatgcttt gaaatatatt ctatcatata ccatatattg tttcaagcat acaaaaaaca
     7081 ggaaaacaag gcggacgaaa aacagattca gatccggata aataattgac aatgtgtaga
     7141 aaaagtgata atttatctta gtattttaaa taaatataag gagtgaagat agtgattgga
     7201 aaaaaatcat ttagtattat tctggttata gctctggttt taggctttat taacccggtt
     7261 tatgccagac aggatgagat tactgtaaac tttaacggtg caataacaat gtttctcgat
     7321 ggtgaagagt tcgttccggt agaaacagac cggacatatc ttactcctgt tatttataat
     7381 ggcaggacct atcttccggt aagggcattg tgcgatgctt tgaaattgta tgttgactgg
     7441 gatgcttcta ccaaaacggt ttctattggc ggcggggagt ggatacctcc tcaaaaggta
     7501 actccgatag gctcgaaaga taaaagcctt cgaacaggta aagcctatag agatactgat
     7561 ttaaaaatgg ttttgtacgg aaggcctttt aatccaaggg aacaagacgg aacgttagta
     7621 tccccgattt tagttaacaa taggacgtat ttacccctca gagcattgtg tgatgcggtt
     7681 ggggtagatg tacaatggga cggagctaca agtacaatta caatgaccag aaatggcaaa
     7741 ggtccgggca aggatggcga atatatacat gatggcagta catacgaaga aaatatatca
     7801 tattatcagg atcttgaacc tatttataat gttcccgtgg atcaggtatt taagtttaag
     7861 ttaaagcttt ccgataaacc ttttgcttct tttggcataa aaaatatgtt taaggttttt
     7921 tccgataggg attgtactac agagttgata gcgaatgtag aatacgatga agagtcaaat
     7981 actatttcaa ttaaacctga tagctcttca tggggaatgt acccgaggga aggcgtcgat
     8041 gttaaaggcc tgtcatgggg aggattgcaa aaatattaca tagttattta tcatgactta
     8101 aatgcgactg aagtaaccga gttgagtaaa cctatgagat taatgtttac agtgggttcc
     8161 gatattccaa tacctaccgt aaaggttaaa aaggataata acggtaatat agttctgacg
     8221 tggaacaaaa ttgagggtgc acaaaaatat aagatttaca gaggcagcag atatggtatg
     8281 gaactcttaa gggaaacaac cgataccagt atgtcaatga ctgaggaaga atatgttgat
     8341 gttgctatga acacattact atcggatgta tataaatatg ccgttacatc agtagtgaac
     8401 ggaaaggaat cccggctgag caatataata tccggagagg actatatttc aatagctccg
     8461 ggtaaaatga ctacattgtc aaaatatatc tcagtaggag acggttttga tgttgacgat
     8521 attttttcac tacccaaatc tgtgagcgtt gattatcttg agtatattcc gaatgatgtt
     8581 cccggaagat acggaagtta taaaacatca aaccataagg taatatggaa tttttcaaag
     8641 aatgtcggtg atgcaaacta tccaagatat gaaggaaaag ttatgggaac tacctttaca
     8701 ctttttgcaa cattttttga taatcctcct acg
//
LOCUS       DAOOJC010000041         8715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_7039_length_8715_cov_3.919861, whole genome shotgun sequence.
ACCESSION   DAOOJC010000041 DAOOJC010000000
VERSION     DAOOJC010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7039_length_8715_cov_3.919861"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="PLA01_02080"
     CDS             complement(<1..1048)
                     /locus_tag="PLA01_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 family protein"
                     /protein_id="HPD00121.1"
                     /translation="MNAITDKLKEVLFSVLPIVIIVLILNFTITPLETPTLIRFLIGA
                     LLIIMGLSIFLLGVDIGITPVGNLMGSSLAKSNKMWIVLTSGAILGFFISIAEPDLHI
                     LAGQVNSVTSGMISKFSIVIIVSIGIAIMLSLGLFRILFNVPLYKVLTGLYALIFVLA
                     LFTTPEFLAISFDASGATTGALTVPFILALAMGVSVLKKDSKASEKDSFGLVAIASAG
                     AIIAVMVMSIVSKADRITSSLEINVSSSNSIIAPFIKALPTILYEVFLALLPLIVIFL
                     VFQKISFKLDKKGFSKIIKGFIYAFLGLVYFLVGVNAGFMEVGTTIGYILASQDNKSY
                     LIFVGFVKGLVTILA"
     gene            complement(1377..2231)
                     /locus_tag="PLA01_02085"
                     /pseudo
     CDS             complement(1377..2231)
                     /locus_tag="PLA01_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
     gene            complement(2261..3646)
                     /locus_tag="PLA01_02090"
     CDS             complement(2261..3646)
                     /locus_tag="PLA01_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HPD00122.1"
                     /translation="MNKVACYVRVSTENQIENYSIEEQTERLKAYCKAKDWTIYKFYI
                     DAGYSGGSTNRPALKQMIEDISQNLINMVVVYKLDRLSRSQKDTLTLIEDEFLAKNID
                     FVSISENFDTSTPFGRAMIGILSVFAQLEKDQITERFTMGRIGRAKSGYFHGGPTPPT
                     GYDYLDGHLVVNEYESMQVREVFERFLKGQSIHSIRSYMSMHYTNKYGNWKSHTLVLN
                     ILRNSTYIGKVKFKGTVYEGNHKPIISEDTFYAAQRLLNSSERENKKTAVQKNPFRAS
                     NLLTGLLICGKCGAGFCGNHGFYTCYSRGKTDKRKVKDPNCKNKKWPIPQLDTVVKNE
                     ILNLDFDKSFFEDKIISEPKPDPDAIKHRIDEFDRQISRMIDLYSIGGIPIEQVSSRI
                     DILKKERNKISDKLKTESKVPLNKVKNIVSNLGAVFKIGTPDEQRLFVRSLIDCITVN
                     DNELDFQWSFQ"
     gene            complement(3727..3885)
                     /locus_tag="PLA01_02095"
     CDS             complement(3727..3885)
                     /locus_tag="PLA01_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00123.1"
                     /translation="MKEKRENASAITIKHHLANKDVRESMKGYKVPINDRTIAAYRIL
                     SRYGKFKE"
     gene            4085..5731
                     /locus_tag="PLA01_02100"
     CDS             4085..5731
                     /locus_tag="PLA01_02100"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPD00124.1"
                     /translation="MRKIRSFVLLPVIALLLNIFSVSFVFGVSYGDLNNDGEIDSTDF
                     TMLKRHILRKLTIDDITAADLNGDGEVDSTDVTILKRYLLRKISSFPVQDNPKDERDE
                     KVIKLENKLKGDIIFSVPSGTFKNQVTVSLSSKIDNAQIRYTTDGTVPTDSSMLYTGP
                     LTFTTTTQLRAQSFVDGNASGDMGTAIYVASSIDAEHDLPVLILDAYGNGKPDREYKD
                     VAFILLEPKNNKVSLLQNPTITTRAGFHIRGQSSANFEKTPYRLELRDNEDDDAKYPM
                     LGMPGDGDWALLSPYPDKSLIRNALAYELGKDLGLDIPRYAHVEVYINFDDQPLSADD
                     YQGVYLLTETLEIDKDRLNIKKLKEDDLTEPNISGGYLMQFNMMAAEEPLIRGNGWSD
                     LELKEPDDLLPEQLAWITNYIQNVHDSIHSANPSDPQTGYPAYIDVDSFVDYIIHTEL
                     GKQPDSYIRSTHIYKDRDQKLKAGPLWDYDLAYDCYPGLFGLPSSNIEGWQYLSTGGT
                     MPGMGNTTDWYYTLMQDPNFRSKVKARWEELRQGPLSDEQ"
     gene            5732..5980
                     /locus_tag="PLA01_02105"
     CDS             5732..5980
                     /locus_tag="PLA01_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00125.1"
                     /translation="MAKVRELSAPLSNAAHRNFQKWNILSTSFVGGFNTQVTQTWEEQ
                     IQIVENYLLKRAEWIDNSGWELSNNPGWPSWPGWGWGQ"
     gene            complement(6110..7045)
                     /gene="cysK"
                     /locus_tag="PLA01_02110"
     CDS             complement(6110..7045)
                     /gene="cysK"
                     /locus_tag="PLA01_02110"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPD00126.1"
                     /translation="MSKIAKNLTDLIGNTPLLELSNYNKENKLEARLVAKLEYFNPAS
                     SVKDRIGYAMIKDAEDKGLINKDSVIIEPTSGNTGIALAFVAAARGYRIILTMPETMS
                     IERRNLLKALGAELVLTPGVEGMGGAIAKAEELAASIPNSYIPQQFNNPSNPEIHRKT
                     TAEEIWRDTDGQVDIFVAGVGTGGTISGVGEILKQRKPGVQIIAVEPSDSPVLSGGNK
                     GAHKIQGIGAGFVPANFNRDVVDEIYKVTNDEAFDTSRRLSKTEGLLVGISSGASAFA
                     ATQIAKRPENKGKLIVALLPDTGERYLSTALFQDA"
     gene            complement(7094..8386)
                     /locus_tag="PLA01_02115"
     CDS             complement(7094..8386)
                     /locus_tag="PLA01_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine synthase"
                     /protein_id="HPD00127.1"
                     /translation="MAERKLKFDTLQVHAGQNVDPTTGSRAVPIYQTTSYVFNSSEHA
                     ANLFALKESGNIYTRIMNPTTDVFEQRIAALEGGVGALGVASGSAAITYAILNIAGSG
                     DEIVSASTLYGGTYNLFSTTLPKLGINTVFVDPDDPENFRKAINDKTKAVYIETVGNP
                     GINLIDIEAVAKIAHENGVPLIVDNTFGTPYLIRPIEFGADIVVHSATKFIGGHGTSI
                     GGVIVDSGKFNWAQSGRFPGFTEPDPSYHGLKYYEALGPLSYITKVRVQLLRDTGACI
                     SPFNSFLLLQGLETLSLRVEKHVSNTKKIVDFLQNHPQVSWVNYPSIKGNKYYELAQK
                     YLPKGAGSIFTFGIKGGINAAKKFIESLEIFSLLANVADAKSLVIHPASTTHSQLSEE
                     EQKACGVTPDQIRLSIGIEDADDLIYDLDQALKKASES"
     gene            complement(8450..>8715)
                     /locus_tag="PLA01_02120"
     CDS             complement(8450..>8715)
                     /locus_tag="PLA01_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPD00128.1"
                     /translation="GGYIISESPSDIKVGRILRVLEGPLSVIDEDSDNTAGSESVIRS
                     CIKTNVWDKMNARLNELVDSLTLEDLARDYRERNGIDNTMYYI"
BASE COUNT         2516 a         1834 c         1716 g         2649 t
ORIGIN      
        1 cagccagtat tgtcaccagg cctttaacaa atccaacaaa aataagataa gatttgttat
       61 cctgtgatgc cagaatatag cctatagtag ttcctacctc cataaatcct gcgtttactc
      121 caaccagaaa atataccaat cctaaaaaag cgtatataaa accttttatg attttactga
      181 accccttttt atccaacttg aaagatatct tttgaaaaac taagaaaata actatcaggg
      241 gaagcaaggc taaaaaaact tcatatagta ttgtgggtaa tgccttaata aagggtgcaa
      301 taatcgaatt cgagctggat acgttaattt caaggcttga tgttattcta tctgcttttg
      361 agacaatgct cattaccata actgctatta ttgcaccggc agatgcaatt gccaccagac
      421 cgaagctgtc tttttcagaa gccttgctgt cctttttcaa tactgaaacg cccattgcca
      481 aggccagaat aaagggcacg gttaacgctc cagtggttgc accggacgca tcaaaggata
      541 ttgccagaaa ctccggggtt gtaaacagtg ctaaaacaaa aataagtgcg tacagtccag
      601 tcagaacttt atagagcggg acattaaaca aaattctgaa caatcccaga gagagcatta
      661 ttgcaatacc aatcgaaaca attatgacaa tactgaattt ggatatcata cctgaagtaa
      721 cagaattcac ctgccccgcc agtatatgaa ggtcgggttc ggcaattgaa ataaaaaaac
      781 caagtatcgc accggaagtt aatacaatcc acattttatt actctttgca agagaagaac
      841 ccataagatt tcccaccgga gtgataccta tatccacccc caataaaaat atggataacc
      901 ccataataat tagcagagcc ccaataagaa acctgataag tgtgggagtt tcaagtggtg
      961 tgattgtaaa attcagaatc agaacaatta ttactattgg aagtacagaa aacaggacct
     1021 cttttaactt gtctgtaatt gcattcaaaa agttcacttc ctttcccctt attttaatac
     1081 cctaaaataa aatcaaataa aaaaggtgtc ttaattttta tttattgctt tgcaaaataa
     1141 aaagttttca ccacttaaag attattttac tgaggacagc taattttata cttctgatag
     1201 gttcaagcgt gaaaagcatt tcgtgctccc cgaaaaacat gaactccccc ttgtttttgc
     1261 tgctatcaag attataacac tgacttgcgt ttcaagcaag ctcatactaa tcctagggtg
     1321 cattaaatga atgtaatgtt ccttccatat tttacttcac ttcaatagta taaaccctat
     1381 aatcccaata acctggctga atttgcaatg ataaagcata ttacaatgcc tgtaaccgtc
     1441 ggtatgagga ttgaagccag ggtccattta agactttgag tctctttttt tatagtccag
     1501 catgttgtac cgcagggcca atgcatgagg gaaaatacca taacacatac tgcagtaagc
     1561 caagtccagc cattggaaat cagaagctct tttaattcat ggaggctctc taattccatc
     1621 atgcttcctt tggacatgta actcattatt ataatgggaa gaactgtttc attggccgga
     1681 aaaccgagga taaaagccat tagtatgtaa ccgtccatac cgatcaacct tgcaaacggg
     1741 tcaagaaaac ctgcacagtg ggtcaaaaga ctgatatcgc caacggttat gtttgccata
     1801 atccagatta tcagcccggc cggtgctgct actgaaactg cccggcccag aacaaacagg
     1861 gttctgtcaa acacagatct tataatgatt tttccgattt gaggcttcct gtatggaggc
     1921 aattccaagg taaaggagga gggaagccct ttaagtattg tttttgacaa tatttttgaa
     1981 ataagcaatg ttgcgacaat gcccaatatt atgactccga ttaatataag tgcacagatt
     2041 atggattgaa aaggagccgc aacggatgct gcaaaaaaaa tggtgataat ggataccagg
     2101 gttggaaaac ggccgttaca cggaacgaag ttgttcgtaa ttattgctat cagtctttcc
     2161 cttggagatt caatgattct gcatccgatt atacctgcag cattgcagcc gaatcccatg
     2221 cacatggtta aaccgataat gtaaatgctg cttgagcacc ctattgaaag ctccattgaa
     2281 agtccagttc attatcattt acggttatgc aatcgataag agatcttaca aaaagccgct
     2341 gttcatctgg ggtaccgatc ttgaacacag cacccaaatt tgaaacaata tttttcacct
     2401 tattaagagg gacttttgac tctgttttta atttatcgga tattttattc ctctcttttt
     2461 ttaaaatatc tattcttgac gatacttgtt ctatcggaat accgccaata ctatacagat
     2521 caatcatcct ggagatttgc ctgtcaaatt catcaatcct gtgttttatt gcatcggggt
     2581 ctggttttgg ctcacttatg attttgtctt caaaaaagct tttgtcaaaa tccaaattta
     2641 aaatctcatt ttttacgaca gtatcaagct gtgggatggg ccatttttta ttcttgcagt
     2701 tgggatcctt gacttttctc ttgtcggttt ttccgcggga atagcatgtg tagaaaccat
     2761 ggttgccgca gaagccagcc ccgcatttac cgcatattaa tagaccggta agcaggttac
     2821 tggcccggaa cgggtttttc tgcacagcgg tttttttgtt ttctctttca gaactgttca
     2881 aaagcctttg tgctgcataa aaagtatctt cggaaataat gggtttgtga ttgccctcat
     2941 acacagtccc cttaaatttt actttgccaa tataggtgct gttacgaaga atattcagaa
     3001 ccagagtatg acttttccaa ttgccgtatt tgttcgtata gtgcatactc atataggatc
     3061 ttatggaatg aatgctctgt ccctttagga atctttcgaa tacttctctt acctgcattg
     3121 attcatattc gtttacaaca aggtgcccgt ctaaataatc gtatcccgtc ggcggagtcg
     3181 gaccgccatg aaaataaccg ctttttgcac ggccgattct tcccattgta aaccgttctg
     3241 taatttggtc tttttcaagc tgtgcaaata ccgacaatat tcctatcatt gcacgcccga
     3301 agggggtaga ggtatcgaaa ttttcgctta tggatacaaa atcaatattt ttagccaaaa
     3361 actcatcttc gataagggtt agcgtgtctt tctgcgaccg gctcaggcgg tcaagcttat
     3421 atacaacaac catattgatt aagttttggc tgatgtcctc aatcatttgc tttagtgccg
     3481 gacggtttgt gcttcctccc gagtaaccgg catcgatata aaatttgtat atggtccaat
     3541 cctttgcttt gcaataggct ttcagccgtt ctgtttgttc ttcaatactg tagttttcta
     3601 tctggttttc agtgctgact cttacatagc atgcgacttt attcatttat actgactccc
     3661 gcataggttt atatatctct caattacttt attcaggact gaagcagtac agctttttat
     3721 aaaaaattat tctttaaatt ttccgtatcg ggacagaatc ctataggctg ctatagttcg
     3781 gtcatttatc gggaccttgt agcctttcat ggattctctg acatctttgt tggcaagatg
     3841 atgcttgatt gtgattgcag aagcgttttc ccgtttctcc ttcatagctt tagacctccc
     3901 tataacttaa tgttatgcac cactgattgt actaaatgta aaaaaattgt tagcacaaga
     3961 taatatgcac ctttcataca atttgaaaag aaagttcata atttttagaa tttaagccat
     4021 aaaacccaaa ttataaacga atacataatt gagtatgatt cttacaatta ttggggggat
     4081 ttgtatgaga aaaatcaggt cgttcgttct gttaccggta attgctttat tgctgaacat
     4141 tttttctgta tcgtttgttt ttggagtctc atatggagat ctcaacaatg acggtgaaat
     4201 agattcaaca gactttacga tgctgaaaag gcatatttta cgtaagctga ctatcgacga
     4261 tattacagct gcagacttaa acggtgacgg tgaagtagat tctacagacg ttacaattct
     4321 aaaaagatac ttattgagaa agatatcttc ttttccggtt caggacaatc ccaaagatga
     4381 acgcgatgaa aaggttatca aacttgagaa caagctcaag ggggatataa tattctctgt
     4441 tcccagcggt acatttaaga atcaggtgac agtttctctg agttccaaaa ttgataatgc
     4501 ccagatccgt tacaccactg acggaactgt tcctaccgat agttcaatgt tgtataccgg
     4561 accattgaca tttacaacca ctacacagct tagggcacaa tcttttgtgg acgggaatgc
     4621 gagcggtgat atggggacgg caatttatgt tgccagttca attgacgccg aacatgatct
     4681 accggtatta attttggatg catatggtaa tggcaaacct gaccgtgaat acaaagacgt
     4741 tgcttttata ttattggagc cgaagaataa taaagtatcc ttactacaga atccgactat
     4801 taccacacgt gcaggattcc atatacgcgg tcaatcctct gccaattttg aaaagacacc
     4861 ctacaggctt gagttaaggg ataatgaaga tgatgatgcc aagtatccga tgctgggtat
     4921 gccgggagac ggggactggg cacttctttc accttatccg gacaaatcgc tgattcgtaa
     4981 tgctttggct tatgaattgg gaaaagactt gggattggat atccctagat acgcacatgt
     5041 tgaagtatat ataaattttg atgatcagcc tttatctgct gatgattacc agggcgtgta
     5101 tttgcttaca gaaaccttgg agatagacaa ggaccgtctt aacattaaga aattaaagga
     5161 agatgatttg acagagccga atatttccgg cggttacctc atgcagttca acatgatggc
     5221 agctgaggaa ccgctgataa gagggaacgg ttggagtgat cttgagctga aagaaccgga
     5281 tgatcttttg cccgaacaat tggcttggat aaccaactac atccagaatg tacatgattc
     5341 aatccacagt gcaaatccat ccgatccgca aacgggctat cctgcgtata ttgacgtgga
     5401 ttcctttgta gattatataa ttcatacaga attgggcaaa caaccggatt cctatatacg
     5461 tagtacacat atttacaagg atcgcgatca gaaactgaaa gccggtcctt tatgggacta
     5521 tgacctggcg tatgactgtt atcccggttt gttcggtttg ccgagttcca atatagaagg
     5581 gtggcaatac ttatctacgg gcgggacgat gccgggaatg ggcaatacca ccgattggta
     5641 ttacacactg atgcaggatc cgaatttcag gagcaaggta aaagcccgct gggaagaatt
     5701 gcgtcaagga cctctatctg acgaacagta ggtggctaaa gtcagggaac tgtctgcacc
     5761 gctatccaat gcagcacacc gtaacttcca gaagtggaat attcttagca cttcttttgt
     5821 aggcggtttt aacacccagg ttacacagac atgggaagaa cagatacaga tcgtagaaaa
     5881 ctacttgtta aagagagctg aatggataga caattccggt tgggagttgt ccaataatcc
     5941 cggctggcct agctggcccg gttggggctg gggtcaataa ttcaattagc taatacacct
     6001 caccttaata ttaaaaagct ccaccgttat ttcggtggag ctacagtttt tcagagttgt
     6061 ttaatgatat gaaggaaaat gtggaagaaa cattctcctt catacttctc tatgcatcct
     6121 ggaataaagc agtagacaga tatctttcac cagtgtcggg aagcaaagcg acaataagct
     6181 ttcctttatt ttcaggcctt tttgcaatct gagttgctgc aaaagcagat gctccggagg
     6241 atattcctac caacaatcct tcggtttttg aaagccttcg tgaagtatca aatgcttcat
     6301 cattagtgac cttgtagatc tcatctacca catctctatt gaaattggca ggtacaaagc
     6361 cagcacctat tccctgaatc ttatgagcac ccttgtttcc tccggataaa accggggagt
     6421 cggagggttc aaccgcaata atttgaacac cgggttttcg ttgtttcaat atttcgccca
     6481 caccggaaat tgttcctccg gtaccgactc ctgctacaaa aatatccacc tgtccgtcgg
     6541 tatctctcca gatttcctca gctgttgttt ttctgtgaat ctcaggattc gaaggattat
     6601 taaactgttg cggtatatag gaattgggta ttgaagcagc cagttcctca gcctttgcaa
     6661 ttgctccgcc cataccttct actccgggag taagcaccag ttcggctcct aatgccttca
     6721 gaagatttct tctttcaata ctcatggtct caggcattgt aagtataatc ctgtaacctc
     6781 ttgcagcagc gacaaaagcc agagcaatac cagtatttcc gctcgtcggt tctattatta
     6841 cactatcctt gtttattaag cctttatctt cagcgtcctt tatcattgca tatccaatcc
     6901 tgtcctttac actggatgca ggattaaagt attccagctt tgcaacaagt cttgcttcca
     6961 gtttattctc tttgttgtag ttggataact ctaaaagtgg ggtattccca ataagatccg
     7021 ttaaattttt tgctattttg gacatataat cgcctcctaa atttacaata tatattataa
     7081 tcgttctatg gctttaagac tccgatgctt ttttaagtgc ctgatccaag tcataaataa
     7141 ggtcatctgc atcttcgata ccaatagaca gtcttatctg atcgggggta acaccgcaag
     7201 ccttttgctc ttcttcagat agctgggaat gggttgttga cgcagggtga attaccaaag
     7261 acttcgcatc ggccacattt gccaagagtg agaatatttc aagactttca atgaatttct
     7321 ttgctgcatt gattccgcct tttattccga aagtaaatat tgagccggct cccttcggaa
     7381 gatacttttg tgcaagctca tagtatttgt tgcctttgat actcggatag tttacccatg
     7441 acacctgagg gtggttttgc agaaagtcca ctatcttttt ggtattggaa acatgctttt
     7501 caacccttaa tgaaagggtt tcaagccctt ggagcaaaag gaaggaatta aacggactta
     7561 tgcatgcccc ggtatccctc aatagctgca ccctgacttt ggttatgtaa gacagagggc
     7621 ctaaggcttc gtaatatttc agaccgtgat agctgggatc cggttctgta aagcccggga
     7681 atcttccgct ttgtgcccaa ttaaactttc ccgaatcaac gatgacacct ccgatggaag
     7741 taccgtgtcc gccaatgaat tttgttgcgg aatgaacaac aatatcggca ccaaactcaa
     7801 tgggccttat caggtatggt gttccgaagg tattgtccac aataagcggc acaccgtttt
     7861 catgggcaat ttttgcaact gcttcaatat caatcaaatt aattcccggg tttccgacgg
     7921 tctcaatata aacagccttt gtcttatcgt taatagcttt tctgaagttt tccgggtcat
     7981 cggggtccac aaaaacagta ttaatcccaa gcttgggcaa tgttgttgaa aacaggttgt
     8041 atgtgccgcc ataaagcgta cttgccgaaa caatttcatc accgcttcct gcgatattga
     8101 gtattgcata ggtaatagcc gctgagcccg aggcaacccc caatgctcca acaccgcctt
     8161 caagtgcggc tattctctgt tcaaaaacat cagttgttgg gttcataatc cttgtataaa
     8221 tattgccgga ttcctttaaa gcaaagaggt tggcagcatg ctcgctgcta ttgaatacgt
     8281 aagaggttgt ttggtaaatt ggaaccgccc gggacccggt agtaggatcc acattttgac
     8341 ccgcatgaac ctgtagggta tcaaatttca attttctttc agccataaga acatctcctt
     8401 tttttaaaat acgattttac ttatcggttt tatcgtgttt aactcataat tatatataat
     8461 acattgtatt atcaattccg ttccgctccc ggtaatcgcg ggccagatct tcaagggtca
     8521 atgaatccac caattcattg agacgggcgt tcatcttatc ccatacattg gttttgatac
     8581 agctgcgaat aaccgactca cttccggcag tattatccga atcttcatcg attaccgaga
     8641 gagggccttc aagaaccctt aaaatccttc ccactttaat atcagaggga ctttcggata
     8701 ttatatatcc accct
//
LOCUS       DAOOJC010000042         8670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_7083_length_8670_cov_3.708880, whole genome shotgun sequence.
ACCESSION   DAOOJC010000042 DAOOJC010000000
VERSION     DAOOJC010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7083_length_8670_cov_3.708880"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PLA01_02125"
     CDS             complement(<1..608)
                     /locus_tag="PLA01_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPD00129.1"
                     /translation="MGNMGRKKDNEKTRTKPKVQFTGKLREKEEQFEDQDRLEGRNSV
                     MEAIKANRTINKILVQKGEKEGSIRQIIAFAKEKGIVVQETDRLNLDRISTTHAHQGV
                     IAYVSFKDYVELDDILKNAESKGEAPFIIILDGITDAYNLGSILRTADAVGAHGVIIP
                     KRRAVGLNAAVSKASAGAIEYMPVARVTNISQTIEYLKKKNI"
     gene            complement(800..1225)
                     /locus_tag="PLA01_02130"
     CDS             complement(800..1225)
                     /locus_tag="PLA01_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HPD00130.1"
                     /translation="MLSELFDKITGEFNFKQEKISQLSPLVLAYIGDAVYEVFIRTML
                     VSEGDMPVHILHKKSIGFVKAKAQSDIIHRIMPSLTPEEHDVVRRGRNTKSGTIPKNA
                     DVTEYKYATGFESLVGFLYLKKDYERLMTILKMAVCEDI"
     gene            1471..3038
                     /locus_tag="PLA01_02135"
                     /pseudo
     CDS             1471..3038
                     /locus_tag="PLA01_02135"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
     gene            3028..4128
                     /locus_tag="PLA01_02140"
     CDS             3028..4128
                     /locus_tag="PLA01_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HPD00131.1"
                     /translation="MKNSKLLPIQVLTLTSSNMLTVNLLTIQQSLVLIAKQDAWISML
                     LGSAIGLVNALAMYYLSSINQGLDLAEMTLYLGGNWFGRLLLSVSVLYVLIYSGLIIR
                     VFVQALQLFLIDRTPMLVVSILLIIVIVSVVSKGIETVAGVIDILFPFFILSLILLIA
                     ISVPQFQIINIRPVLYKNMFNTLKASIPASGALSGFGAIAFTMKYVTEQKKILKWYLS
                     GFFISAGLFILLTASTILVFGPEETQRYLFPTLHLSKSIHVGSILFERLEAFVIILWI
                     PAVFTSVGIYTFTSIRNFVVFFNIKPKHEKYVSLVHIPLLLLVAMFSKNQLEALERMK
                     SFDYIVVCLNFIYIPFLIILTLIKKRRLARVE"
     gene            4121..5350
                     /locus_tag="PLA01_02145"
     CDS             4121..5350
                     /locus_tag="PLA01_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HPD00132.1"
                     /translation="MNRFLSVLLCVSFITFTGCWDSVSIEQRAYIIGIAIDEYPPISG
                     KTESEVESPDTAKEEMFEAATGIHSGSPTYAMTVQLPVIKMSTVPNVQGVGSPDPGTP
                     KTWEITQVGNSFIEMNRALATRINLLPYYGHLQVIIVSDKVAKKGLSNVLDFFARDHE
                     LRSRTRIFITRGEAKKVLDVIPRIEDYSSLYLSETAYNAKLNSQMVHWTDFGKTIQSI
                     YSGRDFLLPIVNSSKDEIMNKGAALFKGDKLVATISGREVEALKMIRNLFDGGIITAK
                     MPNNESSIMALEIDKLKSRITPIIEEDNVAFKIDIKINGDYSENVNCPFTKKIDKSFL
                     EEASEAFEASIKKQCVEAIQKVQSYGTDLFLFNAVLKAEKPSYWDKVKDEWYDIFPHV
                     KTEVNVKVNLKQIGNIQ"
     gene            complement(5486..6895)
                     /gene="cysS"
                     /locus_tag="PLA01_02150"
     CDS             complement(5486..6895)
                     /gene="cysS"
                     /locus_tag="PLA01_02150"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPD00133.1"
                     /translation="MRLFNTLTRKKEEFKPVDEKEVRMYSCGPTVYNYFHIGNARPFI
                     IFDTLRRYLEYRGYNVKFVQNFTDIDDKMIKRAQEENITVKELADKFIGEYFVDAKGL
                     GIKEATVHPRATENIDAIIDIISKLEDKGFAYSVGGDVYFSTGKFKEYGKLSHQSLED
                     LELGSRIDVDERKEDPMDFVLWKAQKPGEPAWDSPWGKGRPGWHIECSAMANKYLGET
                     IDIHSGGQDLIFPHHENEIAQSEAANGKPFARYWLHNGFINVNGEKMAKSRGNFFTVR
                     DIANTFDYEVIRFFMLSAHYRSPINFSEDLLEQAENGLERIYNCLDNLEYLMENAAAA
                     DMTDNERKLRDSLAGLKEKFIEAMDDDLNTADAFAAVFDIVKETNTNINANTNPSKEI
                     IEYAHSLIKELGGVLGIAQKKQDKSIDSEIQELIEKRQMARKEKDWKTADEIRDKLKD
                     MGIILEDTPQGVKWSFRKN"
     gene            complement(6929..7621)
                     /gene="cysE"
                     /locus_tag="PLA01_02155"
     CDS             complement(6929..7621)
                     /gene="cysE"
                     /locus_tag="PLA01_02155"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HPD00134.1"
                     /translation="MIKQFMEDARLIAERDPAARSVAEVVLLYSGFHAVCLHRVAHWF
                     YNRKLYFIARLISQWGRFWTGIEIHPGAKIGKGLFIDHGMGVVIGETAEIGDNCTIYH
                     NVTLGGTGKDRGKRHPTIGNNVLISTGAKVLGPFKVGDNSRIGANAVVLSEVEPNTTV
                     VGVPGRAVKRDGQKLMPSVELDQIHIPDPVAQELCKMCRRVERLEKELAAGQGALTED
                     KANTGEVFDKRE"
     gene            8445..>8670
                     /locus_tag="PLA01_02160"
     CDS             8445..>8670
                     /locus_tag="PLA01_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPD00135.1"
                     /translation="MANYNNARVENFNITHFIIRFVVGTIVLAVTAYLTRGFTISGFW
                     PLIVSAAVIAALDYLVLRFLGMNATPFGRGI"
BASE COUNT         2526 a         1907 c         1502 g         2735 t
ORIGIN      
        1 catatatttt ttttcttcag gtactctatt gtctgggaaa tatttgtgac acgtgccacc
       61 ggcatatact caattgctcc tgctgaagcc tttgaaacag cagcattgag cccaaccgcc
      121 cgccttttgg gtataatcac cccatgtgcc ccaaccgcgt cagcagtcct caaaatggat
      181 ccgaggttat atgcatcggt gataccatca agaataataa tgaaaggtgc ttctccttta
      241 gactctgcat tcttcaatat gtcatcgagc tcaacataat ccttaaaaga aacataggca
      301 ataacccctt gatgtgcatg agtagtagaa attctgtcga gatttaacct gtctgtttct
      361 tgaactacaa tgcccttttc ctttgcaaaa gctataattt gccttatcga gccttccttc
      421 tcacccttct ggaccaaaat cttgtttatc gtcctgttgg cttttattgc ctccataacg
      481 gaatttcttc cctcaagcct gtcttgatcc tcaaattgtt cttctttctc cctcagtttt
      541 ccagtaaatt gtactttagg cttagtgcgg gttttctcat tatctttttt tcttcccata
      601 ttccccataa tctttagtta ccttctctca tttatactaa tgcgtaacag tatctatatt
      661 taagtacgaa aaaagatact tttttatgtc tattattttc tcaaaaaaat acttttttaa
      721 acgaattcca ctgaaaaaaa aacgcccact tccttaaatt ttataaattg cataataatg
      781 ggcttaatta tttatagatt tatatatctt cgcaaacagc cattttcaat atggtcataa
      841 gacgttcata atccttcttt aaatacaaaa aacccaccag agactcaaaa cctgtggcat
      901 acttgtattc cgtaacatca gcatttttgg gaatcgtacc ggacttggtg ttcctccccc
      961 ttcggactac atcatgctcc tccggtgtca aagaaggcat tatcctgtgg ataatatcgg
     1021 attgagcctt tgcctttaca aacccgattg acttcttgtg aagaatatga accggcatat
     1081 ctccttcact gacaagcatt gttctgataa aaacttcata tacagcatcc ccaatatacg
     1141 caagtaccaa gggtgaaagc tggcttattt tctcctgctt gaaattaaac tcacctgtta
     1201 ttttatcaaa aagttctgaa agcatccaaa ttaccttttc catatataaa ataattttat
     1261 tttcttgcca ttaaacacat attaaggcat tactttctgc gtttaatata ttatataata
     1321 caccaatgct ttagtaaata caaatgctaa agttatagga catattattt catttcattt
     1381 catttactta cttttcataa caaatataaa aaaggtaaca atattaaaaa gaaacatata
     1441 tataagagtt aagaaagcag gtataagtat atgttgtcat ggtttaaaaa gaaaatttca
     1501 aaaaacaaaa ccggagctag cactttacat aaattaacag ataaaataaa aaatatctgt
     1561 atatcaaaaa gcattgatag aaatatttgc tctttaaaaa gctttttgaa taacagcccc
     1621 gacataatcg atagaaaaat taattacttt ggcagcaaac ttgacgcagg tatagtattt
     1681 atagatagtc tggtggatgg aaatatggtg gaggagtata tcataaaacc gttaattgct
     1741 gcagatccag tcaaaggtct ctcagtttcg gaattaagca ttgaagcaat caagagttcc
     1801 atactgtacg gatgcagtat tgaggaaatg aatgcaattt atgaaatatc cttggaaata
     1861 ttgagcggta aaaccttctt gtgcatcgaa ggctttgata tggggctgtt aatagatacg
     1921 gcaagttttc caaaacgcag tattaaagaa ccgcgaaccg aaagcacggt caaaggtcct
     1981 agtgaaggat ttgttgaaaa tatgaaagac aatttgggac tcatacgaaa aaggcttaaa
     2041 actccgaatt tatgttttga tcttatcgaa acaggaaaaa ccacacatag taataccgcc
     2101 atattgtatt tgaaaggaag ggttaatacc gaaatccttc aagaagtcaa aaaaagaatc
     2161 aagtccgtta aagactatga tatagtacac atcgagcaac ttgctcacct gatttccgac
     2221 aggtctttta caatatttcc tctgatccat tggacagaaa gacccgataa agcggtttct
     2281 ttcatccttg aaggaaatgt gggaatttta tacgataact cccggggcat tctgttagct
     2341 ccaaccaaca tatcaaccct tatgcagagt cccgatgact actatgagca ttggttgttt
     2401 ggcactgtta taacctttat caggtatata tccctgataa tatcgacttt tcttccggct
     2461 atttacatag cccttacctc ctttcacccg gaaatgcttc caacggctct ggttatttct
     2521 atgtcaggtg cacggctagg agtgccttta ccacccttct ttgaagccct gcttatggtg
     2581 gtaacccttg agatcttgca tgaagcggga ttaaggcttc caaaagttgt cggccaaaca
     2641 gttgccatcg tagggggtct tgtaatcggg caagcagctg tacacgccgg cattataggc
     2701 ccctttatgg tcaccattat atcggtgaca gcgatttctt cctttacaat cccagctaca
     2761 gtctgagcct tgccaccagg atcttaagag tgctctttat gatattggca tctgttttgg
     2821 gtgcctttgg aatttcaatg ggcattatat acttgctcgg atacatgtgt tcccttaaaa
     2881 gctttggatc aggcttcatg gagcccttta ctccttatag gccaagggat tggaaggaca
     2941 atataattcg ggcaccgcag atttttctta aaaaccaacc tgaatacctt gatattaaaa
     3001 gcagttcccg aaaaggaggt tctcaagatg aaaaatagca aacttcttcc tatacaggta
     3061 ttaaccctga cttcttcaaa catgcttacg gtgaacttgc tcaccattca gcaaagcctg
     3121 gttttaattg caaaacagga tgcctggatc tctatgctgc ttggatctgc tattgggctg
     3181 gttaatgcac ttgcaatgta ttacctctca agtatcaatc agggacttga tcttgcggaa
     3241 atgactctgt atttaggtgg taactggttc ggaagactgc tcttatcggt ttctgtctta
     3301 tatgtactta tttattctgg tcttataata agggtttttg tgcaggcctt gcaattgttt
     3361 cttattgata ggactcccat gcttgtggtt tccattctat tgataatcgt gattgtatcc
     3421 gttgtttcaa aaggaattga aaccgtcgca ggtgttattg atatattatt cccttttttt
     3481 attctaagtc taatcctgct tattgcaata tcagtccctc agtttcagat aataaatatt
     3541 agacctgtgc tctacaaaaa catgttcaac actcttaaag caagcatacc tgcatcagga
     3601 gccctctcag gctttggagc catcgcattt actatgaaat atgtaaccga acaaaagaaa
     3661 atactaaaat ggtatttgtc aggctttttt atttcagcgg gacttttcat acttcttacc
     3721 gcttcaacaa ttctggtctt cggtccggaa gaaacgcaga gatatttgtt tccgacattg
     3781 catctgtcaa agtccattca tgttggaagt attctttttg agaggcttga ggcatttgtg
     3841 ataatactct ggatacctgc agtatttact tctgtcggaa tatatacttt tacatccata
     3901 agaaattttg tggtgttttt taacattaag ccaaagcacg aaaagtatgt gtctttggtg
     3961 cacattcccc ttttattact tgttgccatg ttcagtaaaa accaattaga agcattggaa
     4021 cgaatgaagt ctttcgatta cattgtagta tgtttgaatt ttatttatat accgtttctt
     4081 attattctga ctctaataaa aaaaaggagg ctcgcaagag ttgaatagat tcctatcggt
     4141 tctcctatgt gtttctttta ttactttcac aggatgttgg gattctgtat ctatagaaca
     4201 acgtgcatac ataatcggta ttgctatcga tgaatatccg ccgatttccg gaaaaacaga
     4261 atccgaagtt gagtcacccg atactgcaaa ggaagagatg tttgaagctg caacaggtat
     4321 ccactccggc agtcccacct acgccatgac tgtgcaattg cctgttataa agatgagtac
     4381 agtccccaac gttcagggtg taggaagtcc ggaccccggc actcccaaga catgggaaat
     4441 tactcaggtg ggaaactcct ttatagagat gaatcgagcc cttgcaacaa ggataaatct
     4501 acttccttat tatgggcatc ttcaggttat aatagtttcg gataaggttg caaaaaaagg
     4561 cctgagcaat gttctggatt tctttgcccg tgaccacgaa cttagaagca ggacaagaat
     4621 atttataacc cggggcgaag caaaaaaagt acttgatgtt attcccagga tcgaggacta
     4681 ttcttcttta tatctttccg aaacggctta taacgcaaaa cttaattcac aaatggtaca
     4741 ctggacagac tttggcaaaa ccatccagag catttattcg ggcagagact tcttactacc
     4801 cattgttaat agctccaaag acgagataat gaataagggt gcagcactat ttaaaggaga
     4861 taaattggtt gctacgataa gcggccggga agtagaggct ttaaagatga tacgtaattt
     4921 gtttgacgga ggaataatta ctgccaaaat gcccaacaac gaaagctcca taatggcact
     4981 tgaaatagac aaattaaaat cccgtattac tcctataata gaggaagata atgtggcctt
     5041 taaaatagac ataaagatca acggagacta ctcagaaaac gtaaactgtc cgttcactaa
     5101 aaaaatagat aaaagttttc tggaagaagc ctccgaagcc tttgaagcgt caataaagaa
     5161 acagtgtgtt gaggcaatac aaaaagtaca aagctatggc actgacttat ttctgttcaa
     5221 cgcagtgtta aaggccgaaa aaccttcata ctgggataag gttaaggacg aatggtatga
     5281 tatcttcccc catgttaaaa cagaggtaaa cgtaaaagta aaccttaagc agattggaaa
     5341 tattcaatag cttaaggctt taacctttag tactatcaac ataactaatt ctaatgtgaa
     5401 gaataatatt ccataaaatt taagtttcga aagtacaatt tctcccccac atttttcttc
     5461 tccaatacaa tatgtacccg cattatcaat tttttctgaa ggaccatttc accccttgag
     5521 gagtgtcctc cagtattatt cccatatctt taagcttatc ccttatttcg tcggctgtct
     5581 tccaatcctt ttcctttctt gccatctgcc tcttttctat aagctcctgt atttcactgt
     5641 cgatactttt atcctgtttc ttctgagcga tgcccaaaac tccgcccagc tcttttatca
     5701 gggaatgtgc atactcaatg atttcttttg acgggttggt attggcattt atattggtat
     5761 tggtctcctt tacgatatca aaaacagcgg caaaggcatc agcagtgttc aggtcatcat
     5821 ccatcgcctc aatgaatttc tccttgagcc cggcaagact gtcgcgaagc ttcctttcat
     5881 tgtccgtcat atctgcagct gcagcattct ccatcaggta ctcaagatta tcaaggcagt
     5941 tatatattct ctccaaaccg ttttcagcct gttcaagcag atcctcgcta aagtttattg
     6001 ggcttctgta gtgggctgaa agcataaaaa accttattac ctcatagtca aaggtgtttg
     6061 caatatccct gaccgtaaag aaattacccc gggactttgc cattttttca ccattgacgt
     6121 ttatgaagcc gttgtgaagc cagtatctcg caaaaggctt gccgtttgca gcttcactct
     6181 gggcaatctc gttctcatgg tggggaaata tcagatcctg cccaccgctg tgaatgtcta
     6241 tagtctcacc aagatacttg tttgccattg cagaacactc aatatgccaa ccgggtcttc
     6301 cctttcccca cggactgtcc catgcaggtt cgccgggctt ttgagccttc cacagtacaa
     6361 aatccatggg atcctctttt ctctcatcaa catctattct tgagccaagc tccagatcct
     6421 ccaatgattg atgagacagc ttcccgtatt ctttaaactt tccggtgcta aaatacacat
     6481 ctccgcccac actataagca aagcctttgt cctcaagctt tgatatgata tcaattatgg
     6541 cgtcaatgtt ctccgtagcc cttgggtgaa cagttgcttc ttttatcccc aggcccttgg
     6601 catccacaaa atattcccca atgaatttat ccgcaagctc tttaacagta atgttttctt
     6661 cctgtgccct ttttatcatt ttgtcgtcta tatccgtaaa attctgcaca aactttacgt
     6721 tatagcctct gtattcaaga taccttctca atgtatcaaa tattataaaa ggcctcgcat
     6781 tcccgatgtg aaaatagttg tataccgtag gtccgcacga atacatcctg acctcttttt
     6841 catcaacagg tttaaactct tctttctttc ttgtcaatgt gttaaaaagt ctcattaatg
     6901 tttccttcct ctctctcatt catattatct actctctttt atcaaatacc tcaccggtat
     6961 tcgctttgtc ctccgttaaa gctccctgcc ctgcagcaag ttccttctcc aaccgttcta
     7021 ctctgcggca catcttgcag agctcctgtg caacaggatc cggtatatgt atctgatcca
     7081 attcaactga gggcattaac ttttgcccgt ctctttttac cgcccttccc ggaacaccca
     7141 ccaccgtggt attaggctct acctcgctta acaccacagc attggcacca attcttgagt
     7201 tgtcccccac cttaaacggt ccgagaacct tcgccccggt acttataagc acattattac
     7261 ctatggttgg atgtctcttt ccccggtcct tgcctgtacc cccaagagta acattgtgat
     7321 aaattgtaca gttgtctcct atctcagcgg tctcgccgat taccacaccc ataccgtggt
     7381 caataaacag accttttccg attttcgcac cgggatgtat ttcaattcct gtccaaaacc
     7441 ttccccattg ggaaatcagc cgggctataa aatacaattt ccggttataa aaccagtgtg
     7501 caacccgatg gaggcaaacc gcatgaaatc ctgaatacag gagcacaacc tctgccacac
     7561 ttcttgccgc cgggtctctt tcggcaatta accttgcatc ctccataaac tgcttaatca
     7621 tattaatcct cacatccaaa attagtgtat tgaatgatac tcaaaaaaca aattcagccc
     7681 gcaaatatgc attcaataat tttaagtata taatatatat ttaccactca aaccttaatt
     7741 taataaccgt taatattaca ataccggcaa aagctacgga ggtccaccgc ttttttggcc
     7801 ttttccctct gtgcaatccc actaaaaaac cccgccctaa aacatagagg cgaggtaaac
     7861 cgcggttcca ctctaattgg aataatccat ctcatttgca taacggtgcg tcttccgtga
     7921 aagcctacca acccatatat tgcttgtaat acgggattca gccttcaaat tcatgagtgc
     7981 acttcacctg cccattgccc cggaactgct ttcagccgcc gacaattcct ctcttatagg
     8041 gatagttcag ggtacttttc ccaatcatta tctttgccct atcaacttgt tgtttataat
     8101 tttaccatat atatcatcca aggtaaatac aatatttaat ataaactcac ttcgtgggaa
     8161 cggatgctct gcctttcccg catcgtatta acaggccgcc tgcaacttgc atcaaaatct
     8221 ttattccaca tttttcttta tccaaatgat tcgcattcaa ctttccggca atattttaaa
     8281 caaaagtaca gtacacaaac catggttgtg cctgtttgaa taattataat aaaatcaatt
     8341 cataatagta attgtatagt tatatataaa attgcttaat ttaagattaa gcaatttcaa
     8401 acaacttcaa taactatata actattcaga aaggaagtga tcttatggct aattataaca
     8461 acgccagggt ggagaacttt aatataacac attttatcat aaggtttgtg gtaggaacaa
     8521 tcgtacttgc cgtcactgca tacttgactc ggggctttac aatatcaggt ttctggccac
     8581 tcattgttag tgcggctgtt attgctgcac tggattactt ggtattaaga tttttaggta
     8641 tgaatgcaac tccttttgga agaggaatat
//
LOCUS       DAOOJC010000043         8617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_7125_length_8617_cov_2.851904, whole genome shotgun sequence.
ACCESSION   DAOOJC010000043 DAOOJC010000000
VERSION     DAOOJC010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 8617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8617
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7125_length_8617_cov_2.851904"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="PLA01_02165"
     CDS             <1..801
                     /locus_tag="PLA01_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HPD00136.1"
                     /translation="VALAPDEILFNSSMEQKYLVDWIKKGNVLVLIDDWASFEVGLLG
                     LLEYIESTDSIEGYGNNYICTIGKGRVIFLDNYSDYTNGGLEALDPGVVFIDILNEAG
                     HKRVLFNEYYHGIGSSNITLWDILTPAAKILLMQLIIAALILIYVVSRRFGRPQVVYE
                     IVKRKENENLFALSNIYQKSRANGLVLESYLDCLKKELAVFLGFGREGFDDVELLAAA
                     QSNNMLKNFNLRELFGECNYYINNDRKDNREFLSLYKRLEQIRKEIKA"
     gene            798..1790
                     /locus_tag="PLA01_02170"
     CDS             798..1790
                     /locus_tag="PLA01_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPD00137.1"
                     /translation="MSEYMEENRITGNECEFTANKAREIIQEVSRVVVGQYDFIEKLV
                     VSLFSGGHVLVEGVPGLAKTLASKVMAKTIRADFKRVQFTPDLMPADIVGTKVFDQKN
                     GEFYLKKGPLFTNIFLADEINRTPPKTQAALLEGREERAISIDGETHKLEEPFMVLAT
                     QNPVEYEGTYPLPEAQVDRFLMKLIVGYVPQKFENILLQKFNSGFDSRELENENINGV
                     CNPEDIKRCREEIKGVRVSDEIINYITSIIRATRNSPSLILGASPRASIALLLSSKAY
                     AAIQGRSYVIPEDVKDLALPVLRHRIILKPEAGIDGLGNDDVVSSILSKVEVPR"
     gene            1813..3123
                     /locus_tag="PLA01_02175"
     CDS             1813..3123
                     /locus_tag="PLA01_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPD00138.1"
                     /translation="MPFSKLFSLIVAIGIVPVLIGSLVGIELYVFLLYNLIVILLLVV
                     DYRITPGSKAFEVERICDEKLSMGTENEIFINIRNNSDYNVNIEAVDEVPEYMELKKR
                     IVKIKVSPHFENSTSYVVVPQKRGEYTFGKVHIRYNGVLRLCSKKGVYDITKSYKVYP
                     NIKDLSRYGLAAIKKSQLIQGIKKFKAYGTGTEFESLKEYTEGDDYRKINWLASARSN
                     KLIVNTYEPEKNQQVMIMLDSSRVMNSEINYIKKLDYSINAAFLLADVVIKKGDNVGV
                     MVFDSQVKRYIKPGKGLGHFQLLAENLYNVEENFVSADYKGALTYLNQNQNRRCLICI
                     FTELFSVDEAVSLSTSLKNIAKHHVPLLITIKDMRLYEMAQSGVENTGDVYLKAASMK
                     LIEEREKIQRVFIESGIAVLDVPPDRLSIEVVNKYLTMKSMMQI"
     gene            3280..3669
                     /locus_tag="PLA01_02180"
     CDS             3280..3669
                     /locus_tag="PLA01_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HPD00139.1"
                     /translation="MANNESMEMYLETIYLLENNHGHAHVVDIAKQLSVSKPSVTKAI
                     KHLKAQGYVDTEKYGTITLTDKGREKSEEVYRNHQLIELFLVHSLNLSAEQAAINACK
                     IEHVLSEEMIEAIKSYLKKNNISINKL"
     gene            3758..3973
                     /locus_tag="PLA01_02185"
     CDS             3758..3973
                     /locus_tag="PLA01_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPD00140.1"
                     /translation="MTNLANAQVNKEYMIKEVKTDDEELKSFLFTLGCYEGETVTVLS
                     VLGGNLVISVKDARYSIDRDLAKAIVV"
     gene            4167..6170
                     /locus_tag="PLA01_02190"
     CDS             4167..6170
                     /locus_tag="PLA01_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HPD00141.1"
                     /translation="MKIALAGNPNSGKTTLFNAITGKIEHVGNWAGVTVEKKEGNIKK
                     SLNKGNSEIIAVDLPGAYSLSPFTSEESITRDFIKNENPDVIINIVDATNLNRSLFFT
                     TQLLELGIPVVVALNKSDSSKKREIVINAEALSEKLQCPVIETVSTARKKNGLKDLIS
                     AAVNVKGKKQQALFEGLEADLTDKNAVEASDKKRFELVKAIVAEVEIRKTSSNRQTRQ
                     DAVDRVIAHKWFGIPIFAVVMWTVFSISQTYLGPFLADTFVGWIDAFNGWVEGLMGNG
                     VSPVLKAILLDGIIGGVGAVVGFLPLIMVLFFLLALLEDCGYMARVAVVMDRFFKKVG
                     LSGKSIIPMIIGTGCAIPGIMATRTIKNERQRRTTAMLTPFMPCGAKLPVIALFAGVF
                     FNDSAWVGTAMYFVGILIIIIGALIVVRITGEKNARSFFIMELPEYKFPSLKRAIVEM
                     LSRAKAFIVKAGTIILLCNAAVQIMQTFNWKFQVVAEGAENTSILATIASPFAVLLIP
                     LGFGVWQLAAASLTGFIAKENVVGTLAIVYSITNFIDTEELALVDGATDVAGVIGLTS
                     VAALAYLMFNLFTPPCFAAIGAMKAEMENKKWLWGAIAFQLFMGYTVSFITYQVGTLF
                     TTGAFGSGFLPGLIAVIAMIGCVVYLARKGEQEATSKAALKSA"
     gene            6182..6340
                     /locus_tag="PLA01_02195"
     CDS             6182..6340
                     /locus_tag="PLA01_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HPD00142.1"
                     /translation="MENLIVAIVILAIVAVSVTKIVIEKKRGARCIGCPHSKTGGTGC
                     CCNHSAQE"
     gene            6417..7151
                     /locus_tag="PLA01_02200"
     CDS             6417..7151
                     /locus_tag="PLA01_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014522624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HPD00143.1"
                     /translation="MPCPIGTTSYKIQAGDTLYKVAGRYNTTVQQILSVNPGINPNML
                     YIGQEICIPVQSATTAIAEITRIPVLVNGVNINTGIYPVLNYKPPQAQYPYIYVPIAE
                     FGRIGARVVWDEAGQKITVASDCPQAQRQMNIISQALGKLIYDDIAYMDMTEYDNFVI
                     GFWHEIFPYAEFLKITPAWDIRVAQPKFGTGPRKEAHCLVYSGSTSAPPDMQGNLWDP
                     VFVFLYSYSDDKYYLFTSRRPLNLFR"
     gene            complement(7186..8094)
                     /locus_tag="PLA01_02205"
     CDS             complement(7186..8094)
                     /locus_tag="PLA01_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPD00144.1"
                     /translation="MLKKISFFSLISIFITMFFLFSTSFTIRANAETNNNSINYTIKK
                     GDTFYLLSLRFNCSINDISSINPKVDPNNLIIGSKITLPIGSGTTVHHVKKGDTLWKI
                     AKKYSSEINLIAKKNYIENPNLIYSGDILAIPDIKNDQEISTLQKHVISLIRDRNFKE
                     LSKFVHPQKGIKFSPYSYITKQDLVFNASQVANFGSDKTRYLWGIFDGSGFDIKYTPT
                     EYFNRFVYNKDFINLGTVSYNKTQGIGNTFENQFEVYPNSTIVEYYYPGSEKYSGIDW
                     TSLRMVFEKYNGKWYLVGIIHNEWTT"
BASE COUNT         2805 a         1346 c         1902 g         2564 t
ORIGIN      
        1 gttgctttgg cacccgacga aatccttttt aacagcagta tggagcagaa atatctggtg
       61 gattggatta aaaagggaaa tgtacttgta ctgatagatg actgggcaag ctttgaagta
      121 ggtttgttag gccttttgga atatattgaa agtacagatt ccattgaggg atatggtaat
      181 aattacatat gcaccatagg aaaaggacgg gtaattttcc tggataatta tagtgattat
      241 accaacggcg gacttgaggc cctggatccc ggggtggtat ttatcgatat ccttaatgaa
      301 gcggggcata aaagggtgtt gttcaatgag tattatcacg gtattggcag ttccaatatt
      361 acattgtggg acatattgac tcctgcagca aaaatactgt tgatgcagct gataatagcg
      421 gcattgattc ttatatatgt tgtatcaaga aggtttggaa gacctcaggt ggtatatgaa
      481 attgtaaagc gtaaggaaaa cgaaaacctt tttgccctct caaacatata tcaaaaatcc
      541 agggccaacg gtcttgtttt ggaaagctat cttgattgtt taaaaaagga gcttgctgta
      601 tttttaggct tcggaaggga aggctttgat gatgttgagc ttttggctgc ggctcagtcc
      661 aacaatatgc taaagaattt taatttaaga gaattgtttg gagagtgcaa ctattatatt
      721 aacaacgata gaaaggataa cagagaattt ttaagtctat ataaaagatt ggagcaaata
      781 agaaaggaga taaaagcatg agtgaatata tggaagagaa tcgtataacc ggcaatgaat
      841 gcgaatttac agctaataaa gccagggaaa taatccagga ggtatcccgg gtagttgtcg
      901 ggcagtacga ttttattgag aaattagtgg tttccctgtt ttccggaggg catgtactgg
      961 tggaaggagt tccgggactt gctaaaacac tggcttcaaa ggttatggca aagacaataa
     1021 gggcggattt taaaagggta cagtttaccc ctgaccttat gcctgcagat attgtaggaa
     1081 caaaagtttt tgatcaaaaa aacggggaat tttaccttaa gaagggtcct ctctttacca
     1141 atatatttct tgccgatgag ataaacagaa ccccccccaa gacccaggca gcacttttag
     1201 aaggcaggga ggagagggca atatcgattg acggtgaaac ccataagctg gaagaaccct
     1261 tcatggtact ggcaactcag aaccctgtgg agtacgaagg aacatatccc cttcccgaag
     1321 ctcaggttga caggtttctt atgaagctga ttgttgggta tgttccacag aagtttgaaa
     1381 acatcctttt acagaaattc aattccggct ttgacagcag ggagcttgaa aatgagaata
     1441 taaacggggt atgcaatccc gaggatataa agaggtgccg ggaggagata aagggtgtta
     1501 gggttagtga tgaaataata aactatatta catcgataat aagggcaaca agaaactctc
     1561 ccagcctgat tttgggtgca agccccaggg cttccatagc actgttgctt tcttcgaaag
     1621 cctatgctgc cattcaggga aggagttatg ttattcctga agatgtaaag gatctggcac
     1681 ttccggtact ccgtcacaga ataatattaa agccggaagc gggcattgac ggtcttggaa
     1741 acgatgatgt ggtcagcagt attttatcga aggttgaagt accgagataa ttatttagaa
     1801 ggagaagtat aaatgccttt tagcaagctt ttttctttaa ttgttgcaat tggaatagtg
     1861 ccggttttaa taggatcctt agtaggaata gagctgtatg tttttttgct ctataacctt
     1921 attgtcatcc tgcttttagt tgtggactac aggattactc ccgggagcaa agcatttgag
     1981 gtagagcgta tttgtgatga aaaactctca atgggaacgg agaatgaaat tttcataaat
     2041 attcgaaaca acagtgatta taatgtaaat atagaagcag ttgatgaagt tccggagtat
     2101 atggaattaa aaaaacgtat tgtaaaaata aaggtttcac cccattttga aaacagcaca
     2161 agctatgttg tcgtgccaca aaagaggggg gaatatacct tcggaaaggt tcatataagg
     2221 tacaacgggg ttcttaggct atgctcgaaa aaaggtgttt atgacattac aaaaagctat
     2281 aaggtgtatc ccaatataaa ggatttgagc aggtatggac ttgcagccat aaagaaaagc
     2341 caactgattc agggaataaa gaagtttaaa gcctacggta caggtaccga atttgaaagt
     2401 ctgaaggaat acaccgaggg cgatgactac agaaaaataa actggcttgc ttccgcaaga
     2461 tcaaacaagc tgattgtcaa tacctacgag ccggaaaaga accagcaggt aatgattatg
     2521 ctcgattcga gcagggtaat gaattcggaa ataaattata taaaaaagct tgactattca
     2581 ataaatgcag cttttttact tgccgacgtc gtaataaaga agggtgacaa tgtgggagta
     2641 atggtttttg acagccaggt aaagcgttat atcaaaccgg gcaagggtct cggtcatttt
     2701 caactgttgg cggaaaacct gtacaatgtg gaagaaaact ttgtttcggc agactataaa
     2761 ggtgcattga catatttaaa ccaaaaccag aacagaaggt gtctgatttg tatttttacg
     2821 gagttgttca gtgtggatga agctgtttcc ctttcaacgt cattgaagaa cattgcaaag
     2881 catcatgttc ccctccttat aacaattaag gacatgaggc tttacgaaat ggcacagtcc
     2941 ggcgttgaaa acaccggtga tgtttatctg aaggcggctt cgatgaagct gattgaggag
     3001 agggagaaga ttcaaagggt gtttatcgag tccggaattg cagtgttgga tgttccgccc
     3061 gacaggctgt ctattgaggt tgtaaacaaa tacctcacca tgaaatccat gatgcagata
     3121 taaaaagtta aaaagcggta attgagattt aaaagaatag aatgatgcat tcatagataa
     3181 attttccttg acaaagggaa tttattttgc tatgcttaaa ttgttaaccg aggttaactt
     3241 atcggtgaaa ttattaagta ttattaatgg agtgttaaag tggctaataa tgaatcgatg
     3301 gaaatgtatc ttgaaacaat atatttattg gaaaacaatc acggtcatgc ccatgtagta
     3361 gatattgcaa aacaacttag tgtttcaaag cccagtgtga caaaggctat aaaacatctt
     3421 aaagcccagg ggtatgttga tacggagaag tatggaacaa ttacattgac agataaaggc
     3481 agggaaaaat ctgaagaagt ttataggaac catcaattga tagaactttt tttagtgcat
     3541 tctctcaatt tatctgctga acaggccgct atcaatgcat gtaaaataga gcatgttctc
     3601 agtgaagaga tgattgaagc aattaaaagc tatttaaaga agaacaatat cagtataaat
     3661 aagctgtaaa gcatgagggg gtttacttat ggctcataca atattaagta aaataatatt
     3721 gccggtcaat gagagaactg aaaggaatac tgacaaagtg acaaatttgg caaatgcaca
     3781 ggttaataaa gaatacatga tcaaagaagt gaaaaccgat gatgaggaac ttaagagttt
     3841 tctgtttaca ttaggatgtt atgagggtga aacagtgact gtactgtcag tactcggcgg
     3901 taatttagtt atttcagtaa aggacgcaag atatagtatc gatagggatc tagcaaaagc
     3961 aattgttgtg tagttttttt atggcattta agttaactgt agttaacttt tacgggtatt
     4021 agtgggattt ctaacgagtg aaataacaaa aatacgatga ggacctgatg ctcttggaac
     4081 ttaaatcgtt aaattcttaa caatttttat gcaaaaagag ttaaccgtag ctaacattat
     4141 gtcttatatt aataaggagg aactttatga aaatagcact agcgggcaat ccaaacagtg
     4201 gaaaaacaac attattcaat gcgataacag gtaagattga gcatgtggga aactgggcag
     4261 gtgtaacagt tgagaagaag gaagggaata tcaaaaaaag tcttaacaaa gggaattctg
     4321 aaattatagc agtagacttg cccggtgcat attctttgtc accattcaca tccgaggaaa
     4381 gcatcacaag agacttcatt aaaaatgaaa atcctgatgt gattattaat atcgttgatg
     4441 ctacaaactt gaacagaagt ttgttcttta caacacagct tctggagctg ggtataccgg
     4501 tggttgttgc ccttaacaag agtgattcga gcaagaaaag ggagatcgta atcaatgcag
     4561 aggctctgtc cgaaaaactt cagtgtcctg ttattgaaac agtatctact gccagaaaaa
     4621 aaaacggcct aaaagacctt atatctgctg ccgtaaatgt taagggcaag aagcagcagg
     4681 cactctttga ggggttagag gccgatttga cggataaaaa tgcggttgaa gcatcggata
     4741 aaaaacggtt tgaacttgtg aaagctattg ttgcagaggt tgagatcaga aaaacaagca
     4801 gcaaccgtca aacaagacag gatgcagttg acagagttat cgcacataaa tggttcggaa
     4861 tcccaatctt tgcagtagtg atgtggacgg tgttttccat ttcacagact tatttgggac
     4921 cgttcttggc cgatacattt gtcggatgga tagatgcatt caacggatgg gtagaaggat
     4981 taatggggaa tggggtatca cctgtgctta aagccattct cttagacggt attattggcg
     5041 gggttggtgc tgttgtaggt ttcttgcccc ttatcatggt attgtttttc ttgctggcac
     5101 tattggaaga ttgcggatat atggcacggg tggcagtcgt tatggatagg ttttttaaga
     5161 aagtaggatt atcgggaaaa tcaattattc caatgattat cggtacagga tgtgctatcc
     5221 cgggaattat ggcaacgaga acgataaaaa acgaacggca aagaagaacc actgccatgc
     5281 ttactccttt tatgccatgc ggtgctaaat taccggtcat tgccctattt gcaggtgtgt
     5341 tctttaatga ttcggcttgg gtgggaacgg caatgtattt tgtgggtatt ttaattatta
     5401 tcataggtgc attaattgta gtaagaatta ctggagagaa aaatgcaaga tccttcttca
     5461 ttatggaact gccggaatat aaatttccaa gcctaaaaag agctatagta gaaatgcttt
     5521 ccagggcaaa agcatttatc gttaaggccg ggacaattat tcttctttgt aatgccgcag
     5581 tgcaaattat gcaaaccttt aattggaaat tccaagtagt tgctgagggt gcagagaata
     5641 ccagtattct cgccactatt gcatcgccct ttgcagttct tttgataccg ttgggattcg
     5701 gtgtatggca gttggctgca gcatcgctta ccggatttat tgcaaaagag aatgtagtcg
     5761 gtacattggc tattgtgtac tcaatcacaa acttcattga tacggaagaa ttggccctgg
     5821 ttgatggagc gactgatgtg gcaggcgtaa taggattaac atcggtagct gcattggcat
     5881 atcttatgtt taacttgttt acaccgccgt gttttgccgc tattggtgct atgaaggctg
     5941 agatggaaaa taaaaagtgg ctttggggtg caattgcatt tcaattgttt atggggtata
     6001 ctgtttcatt tattacttat caggttggaa ctctatttac aacaggtgct ttcggcagtg
     6061 gctttttacc gggtttaatt gctgtaatcg caatgattgg ctgtgttgtt tatcttgcac
     6121 gaaaaggtga acaagaagca acttcaaaag cagcactaaa aagtgcatag gaggaatcaa
     6181 tatggaaaat ttaattgtag caattgttat tttagcgatt gtagctgttt cagttactaa
     6241 aatcgttatt gaaaagaaaa gaggggccag gtgtataggt tgtccccaca gtaaaacagg
     6301 gggaaccggt tgctgttgca accattccgc tcaagaataa aaaggatata ctttcactaa
     6361 tcatcttcta ttaacatatt atgaaataat actttatgtt taggagatga gtgaaagtgc
     6421 catgtcccat tggaactaca agctataaaa tacaggctgg tgatacacta tataaagtag
     6481 ccggtagata taatacaacg gttcaacaga tactgtcggt gaacccgggt ataaatccca
     6541 atatgcttta tatcggacag gaaatatgca ttcccgttca atcggctacc acagccatag
     6601 cagagattac aagaattcct gtattggtaa atggagttaa tattaatact ggaatatatc
     6661 cggttctcaa ttataaacca ccgcaggcac aatatcctta tatttatgtt ccaatagcag
     6721 agttcggaag aataggtgca agagtggtat gggatgaagc tggacaaaaa ataacagttg
     6781 caagtgactg tccccaagct caaagacaaa tgaatattat ttcacaggct ttaggcaaac
     6841 tgatttatga cgatatcgcg tatatggata tgactgaata tgacaatttc gtgataggtt
     6901 tttggcacga gatttttccc tatgcagaat ttcttaaaat aactcctgct tgggatatca
     6961 gagtagcaca accgaagttt ggaaccggac cacgaaagga agcacattgt ttagtgtatt
     7021 caggaagcac ttcagcacca cctgacatgc aagggaattt gtgggatcct gtatttgtat
     7081 tcctatatag ttactcagat gacaaatatt atttatttac atcccggcga cctttaaatc
     7141 tattcagata aaagtatgca aatggggcag atactgcccc aatgcttatg ttgtccattc
     7201 attgtggata atgcccacaa ggtaccattt tccgttatac ttttcaaaaa ccatacgcag
     7261 actggtccaa tctataccgg aatatttttc gcttccagga tagtagtatt ctacaatggt
     7321 actgttcgga tacacttcaa actgattttc gaaagtattt ccaattcctt gagttttgtt
     7381 atagctcact gttccaaggt tgataaagtc cttattatac acaaacctgt taaaatactc
     7441 agtaggtgta tattttatat caaaccctga accgtcaaaa attccccaaa gatatctggt
     7501 tttatccgag ccaaagtttg ctacttgaga agcattaaac actagatcct gctttgtgat
     7561 ataggaataa ggcgaaaact taataccttt ttgtgggtga acgaattttg acagctcttt
     7621 aaaattcctg tctcttatca gactaataac atgcttttgc aaagttgaaa tttcctgatc
     7681 gttcttgatg tcgggtattg caagtatatc accggagtat ataaggttcg ggttttctat
     7741 ataattcttt tttgctatta gattaatttc actgctatac ttttttgcaa ttttccatag
     7801 agtatcacct ttttttacat ggtgtacggt tgttccggaa cctatgggca acgtaatttt
     7861 actgcctatg attagattat tcgggtcaac ctttggattt attgatgaaa tatcattaat
     7921 cgagcaatta aatcttaagc tcagaagata gaatgtatct ccctttttta tagtataatt
     7981 tattgaatta ttgttggttt ctgcattagc acgtattgtg aatgaggttg agaaaagaaa
     8041 aaacattgta ataaaaattg aaatcaaaga aaaaaagctt attttcttta acactattaa
     8101 ttcctccttt ctttgttgtt tatataaaaa tacaaatatt attattagca atacagctta
     8161 aaatatttag agaccgttgc ctagaaaaaa attcaatata gatctttaca atttatcaat
     8221 agaacggaaa atcaagctga tgaaactaca aagataaaat tggtaaagat aatagtttac
     8281 aagctaggtt gtttatgcta atatttaaaa tggtttttca tttataagta aattcaatat
     8341 aaccgtattg ataaaaagtg atgtaattat atgtgatgat aacggtataa tttaataaaa
     8401 gagaaattgg tgcatatgat attttatcaa taaaaaaggt gggaaattgc tgtgaaaaat
     8461 agtggcaggt cagatttttt aataattttt gcaatcttat tagttgtact tatttcttgt
     8521 ttcatatttg ctatatatca agaagttaaa aaaatagatg taaaatatga ggattaccgt
     8581 gagggattag aagaaacaga ctattcaaat gccgtac
//
LOCUS       DAOOJC010000044         8599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_7138_length_8599_cov_3.615871, whole genome shotgun sequence.
ACCESSION   DAOOJC010000044 DAOOJC010000000
VERSION     DAOOJC010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 8599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8599
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7138_length_8599_cov_3.615871"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLA01_02210"
     CDS             complement(<1..337)
                     /locus_tag="PLA01_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HPD00145.1"
                     /translation="MDVLITIEGTIEEIIFSNEINGYTVCEIRKDKDVITAVGFMPFV
                     NAGEVLKICGRWVTHPDYGEQFKVEMYEKMLPKTTEAIEKYLSSGLIKGVGPATAKKI
                     VKRFGEQTIE"
     gene            complement(470..1141)
                     /locus_tag="PLA01_02215"
     CDS             complement(470..1141)
                     /locus_tag="PLA01_02215"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HPD00146.1"
                     /translation="MKNLEEYINEEVGLEVINEEGEKIGPSYKTKIVEMLDEDNIGIV
                     APTFDKYTIPLPVDTKVKIIFKTAYERVNFIIGAIVSKSKSESEILHRVRINIPKPNE
                     ITNTSSVRVDCNLKAEYGLVDLSDEKEFKPAKVVSISAKGLSLSVNEEIEPDKSVEVY
                     LWISPKKIISGVCEITQKSETANPKEFKYKLALEFTEICEPDKDAIIKFIFTKQKEDI
                     KANNT"
     gene            complement(1188..1673)
                     /locus_tag="PLA01_02220"
     CDS             complement(1188..1673)
                     /locus_tag="PLA01_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00147.1"
                     /translation="MLYTLIEKCKPFLGNYSEIYNLEYIYEMAEYLKTTFYIDEKKRV
                     SGNILLNGAMNSEIKAIGNVVISGKTCFNTKIEAGGKVMISGNLRLGEIKAEKGIEAN
                     RVGTEIGTKIFLEVSDKGFIRMKSVYPDTTIRIGRHTYKFDSYQTGINARVVKNKLVL
                     K"
     gene            complement(1720..2961)
                     /locus_tag="PLA01_02225"
     CDS             complement(1720..2961)
                     /locus_tag="PLA01_02225"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPD00148.1"
                     /translation="MSDNSAAEITEKNEDGYAWIIDGKLFVKDPVGKGIQPLRNPCEG
                     IKLIVNGIETNHLVMVSEKDNIEIKTTDEEAEVEADVKISEDKLKAYMSFKPPRKIKR
                     VLKDSYPVNKLDISLMEEEDFQDGYSVEQLIDIIRQQGVVYGLKEDIIRKAYKENKKG
                     RYLIAEGKPPIDATDDNMEFFLEDIEEQAINEYENIDHKELIKYKSVKPGEKIARLIR
                     GNPGEEGISVLGETIKPKEARKIIIEASRDIQFDEESGMIRARKFGTPVKKEKGNVIT
                     YEISEKLQLVEVSLKTGNVKFKGDVEITGSVYEGMKVIANNNIFIFGDVNFSSLYSGN
                     DIVVKGNVVTSQLVSGDDTIASKAVNTEIHRIIAEIENLIDNIKKESVSELNSQGIKG
                     MPEIVRYMLNTKKQSSAHYHI"
     gene            complement(3157..3555)
                     /locus_tag="PLA01_02230"
     CDS             complement(3157..3555)
                     /locus_tag="PLA01_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00149.1"
                     /translation="MLQIALEVLVCVLAAYGLITLIFESLLSARHNRDYKNSMVKLVL
                     IVKNQGESIEGVLRNVLPRDFIRKLMPGGKLIVLDMDSKDDTLDILRKLERDYECLEV
                     LKRSEKEFLFKPFEEADDKGTVIEHAGKNT"
     gene            complement(3684..4880)
                     /gene="metK"
                     /locus_tag="PLA01_02235"
     CDS             complement(3684..4880)
                     /gene="metK"
                     /locus_tag="PLA01_02235"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPD00150.1"
                     /translation="MTKKLFTSESVTEGHPDKICDQISDAILDAIYSEDPMARVACET
                     SVTTGLVLVTGEITTKCYVDIPKVVRKTIREIGYDRAKYGFDCDTCAVLTSIDEQSPD
                     IAMGVDKALEAKTGEMTDAQIEAIGAGDQGMMFGFACDETPELMPMPISLAHKLTRRL
                     TDVRKKGTVEYLRPDGKSQVTVEYDGDTPVRIDTVVISTQHGADVDHETIEKDIMEHV
                     IRPVIPANLIDEKTKFFINPTGRFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLAKKCEVQLAYAIGVARPVSVRIDTFGTGSIPD
                     EKIESIVTELFDLRPAAIIQTLNLRRPIYKQTAAYGHFGRDDLDLPWEKTDRVEDIKK
                     KVAAMQ"
     regulatory      complement(5040..5143)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(5294..7096)
                     /gene="ftsH"
                     /locus_tag="PLA01_02240"
     CDS             complement(5294..7096)
                     /gene="ftsH"
                     /locus_tag="PLA01_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPD00151.1"
                     /translation="MKYFRNISFYIVLFVMLLAFLVVIRIGPDPKKGKYSDLIRDIYD
                     GKVQEVVLEGDKATVKYKDKQVKEQYVYIPDTPTFMNEINSFIVEESLIVDSAPPPTP
                     PWWLSIIPTLAVILIFVLFWVFFLQQSQGGGGNRVMSFGKSRAKMTIDDKKKVTFNDV
                     AGADEEKEELKEIVEFLKHSKKFLELGARIPKGVLLVGPPGTGKTLLAKAVSGEAGVP
                     FFSISGSDFVEMFVGVGASRVRDLFDQAKKNAPCIIFIDEIDAVGRHRGAGLGGGHDE
                     REQTLNQLLVEMDGFGVNEGVIILAATNRPDILDPALLRPGRFDRRVVVGLPDIKGRE
                     EILKVHAKGKPLAEDVKLDELAKSTPGFTGADLENLLNEAALLAARNNKTMITMSEIK
                     EAVFKVVMGPEKKSRVMSDKEKRLTAYHEAGHAIAVKAVSTTDKVDRISIIPSGMAGG
                     FTAHKPDEDKNYHTKSHLLEKIVVSLGGRAAEEIVLGEISTGAYSDLKQANSIARSMI
                     TKYGMSDKLGNLIFGNENDEVFIGRDFAQTRNYSEEVAAQIDREVKHIIDTCYQKIKN
                     ILNENINKLHTVANVLIEKEKIEGHEFEELYANA"
     gene            complement(7242..7793)
                     /gene="hpt"
                     /locus_tag="PLA01_02245"
     CDS             complement(7242..7793)
                     /gene="hpt"
                     /locus_tag="PLA01_02245"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPD00152.1"
                     /translation="MKEEIEEILIGREELKNNARELGKRISSDYEGRELVLIGVLKGG
                     VVFFSDLIREITIPIEIDFISVSSYGNSTKSSGVVRIIKDTDIDVTGKHVLIVEDLVD
                     TGLTLHYLKDLFKARKPKDVKICTALDKPSRRKVEIEIEYRGITIPDKFVVGYGLDYG
                     GKYRNLADVCVLKPWVYKDGTES"
     gene            complement(7896..>8599)
                     /gene="tilS"
                     /locus_tag="PLA01_02250"
     CDS             complement(7896..>8599)
                     /gene="tilS"
                     /locus_tag="PLA01_02250"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPD00153.1"
                     /translation="VYNEINIEIKNNEATLNIKEIRGYHIAVVRRVVRNLIKEIRGNL
                     RGIENVHVDSVADLIENGRTGAMLHLPGGLRALKSYETLKIYLCEKECCVRDFEKRVV
                     IPGITCIDEINSSVEAIVIDVPEGVKDYIKTKGDSLVQFFDYDRLKDGINIRYRRDGD
                     VFKPLGSTGTKKLKEFFIDSKVQRHLRDQIPLISKDKEIVWIIGYRISDKFKVTENTK
                     TILKLYFKRETNTDN"
BASE COUNT         2271 a         2071 c         1250 g         3007 t
ORIGIN      
        1 tttcaatggt ttgctcacca aaccttttta cgatcttttt tgccgttgcc gggccaaccc
       61 ccttgataag tccggaagaa aggtattttt cgatggcctc ggtagtcttg ggaagcatct
      121 tttcgtacat ctcaacctta aactgctcac cataatcggg atgagtaacc cacctgccgc
      181 aaattttcag aacctcaccg gcattcacaa agggcataaa tcccactgcc gtaatgacat
      241 ccttatcctt tctgatttca caaaccgtat atccgttaat ctcattggag aatattatct
      301 cttctatagt accttctatt gtgatcaata catccacctg attctttata taattcaact
      361 gttatatata aagtatatat tataaatgtg ttttagaaaa gaacaggctc ttaaacttgc
      421 atcacaatat ttcttccata ttttcttcgc ccaaataata acaaccggct tatgtattgt
      481 ttgcttttat gtcttctttt tgttttgtaa atatgaattt tattatggca tctttatccg
      541 gctcgcaaat ctctgtaaat tccagtgcta acttatattt gaattccttc ggatttgccg
      601 tttcgctttt ttgtgtaatt tcgcatactc cactgattat ttttttagga ctaatccata
      661 aataaacctc caccgattta tccggttcga tttcttcgtt taccgataaa cttaagccct
      721 tcgcactaat acttactact tttgcaggct taaattcttt ttcatctgac aaatccacca
      781 gaccgtactc tgctttcaaa ttacaatcta ctcttactga agaagtgttt gttatttcgt
      841 ttggttttgg aatgtttatt ctgactcgat gtaaaatttc gctctcactt ttagattttg
      901 aaactatcgc accaataatg aagttaactc tttcatatgc tgttttaaaa ataattttca
      961 cctttgtatc taccggcaat ggaattgtat atttatcaaa tgtcggtgct actatgccta
     1021 tattatcttc atcaagcatc tcaacaattt tagttttata tgaaggtcct attttctcgc
     1081 cttcctcatt tattacttcc aaccccactt cctcatttat gtattcctcc aaatttttca
     1141 tatcgttaaa tcacttcctt acattaagtt ttgttatttc ccggtaatca ctttaaaacc
     1201 agcttatttt taactactct tgcatttata cccgtttggt aactgtcgaa tttatatgta
     1261 tgtcttccaa tcctgattgt cgtatcggga tatacagact tcatacggat aaaaccttta
     1321 tccgaaactt caagaaaaat ttttgtacct atttctgttc caaccctatt agcttctatg
     1381 cccttctcag ccttaatttc tcccagcctt aaatttcccg atatcataac cttcccgcca
     1441 gcctctattt tggtgttgaa acaggttttg cccgatatga caacattacc gatagctttt
     1501 atttccgaat tcattgcacc atttaaaaga atattaccgc ttaccctctt cttttcatct
     1561 atataaaatg tggttttaag atactcagcc atttcataaa tgtactctaa attataaatt
     1621 tcactgtaat ttcctaaaaa gggcttgcat ttttctatca acgtgtatag catactgtca
     1681 tctatatcat aattatcttt cttaaattta tgtattatat catatatggt agtgggcaga
     1741 gctctgtttt tttgtattaa gcatatatcg tacgatttcc ggcataccct taattccttg
     1801 tgaattaagt tcactaaccg attctttttt aatgttatca ataagatttt ctatttcggc
     1861 aataatcctg tgtatttcag tattaaccgc cttcgatgct atggtatcat ctccggaaac
     1921 caattggctg gtaacaacat ttcccttaac aactatatca tttcctgaat acaaagatga
     1981 aaaatttaca tctccaaata taaatatgtt gttatttgca attaccttca tcccctcata
     2041 tacactgccc gttatttcca catctccctt aaatttcaca tttcctgtct tcaaactgac
     2101 ttctactaat tgcaattttt cagatatctc ataagttatt acattgcctt tttccttctt
     2161 tacaggagta ccaaattttc tggctcttat cataccgctt tcttcatcaa attgtatgtc
     2221 tctgcttgct tctataatta tcttccgagc ttctttcggc tttatagttt cacctaacac
     2281 tgatattcct tcctcaccgg gattccccct gattaaacgg gcaattttct ctccgggttt
     2341 gaccgattta tatttgataa gctctttatg atcaatattt tcatactcat taatggcttg
     2401 ttcttcaata tcttctaaaa agaactccat attatcatct gtggcatcta tcggtggttt
     2461 cccttctgct atgagatacc tgcctttttt attttcttta tatgccttcc tgattatatc
     2521 ttccttcaac ccatagacca caccttgctg ccttattatg tcaataagtt gttctaccga
     2581 ataaccatct tgaaaatcct cttcttccat caacgatata tcaagtttgt ttaccggata
     2641 ggagtccttt aaaacccttt ttattttacg cggtggttta aaagacatgt aagccttcaa
     2701 tttgtcctca gatatcttga catctgcttc cacttccgcc tcttcatcgg tagtctttat
     2761 ctctatattg tccttttcgg aaaccataac aagatgattt gtttcaatac cgtttacaat
     2821 cagtttgatg ccttcacacg ggttgcgcaa gggctgtata ccttttccca ccggatcttt
     2881 tacaaataac tttccatcga ttatccatgc atagccatct tcgttttttt ctgttatttc
     2941 agcagcacta ttgtcagaca taagtaacac ctcgctaatt attgattcat tttagttttt
     3001 gttactgtat tgttttttct tatctttttg ctagttttaa tattctaata atatatcgaa
     3061 ccattattaa ttttttttag tatatttatg ctttatttta ggttatttgt attgagtaaa
     3121 taatgaacgg tttcgtcaac taatagatta atatatctat gtatttttac ctgcatgttc
     3181 tataactgtg cccttatcat cggcttcttc aaatggtttg aataaaaatt ccttttcact
     3241 tcttttcagt acctcaaggc actcataatc cctttccagc tttcttagaa tatccaacgt
     3301 atcgtccttg gaatccatat caagtacaat gagcttccct cccggcataa gctttcttat
     3361 gaaatccctg gggagaacat ttctcaatac accttcaatg gactctcctt gattttttac
     3421 tatgagcacc agctttacca ttgagttttt atagtccctg ttatgcctcg cagacagtaa
     3481 tgattcaaat atcagtgtta tcaggccata ggctgcaaga acacatacca atacttccaa
     3541 agcaatttga agcataaaaa aaacaccccc aacaacagcc tatgaaatag gctattaaac
     3601 tgttacaggt gttttcttca aatattaatc gtgcagagca caaatgaaga aaaaaattct
     3661 atgaaattaa atttcgcagt attttattgc attgctgcga cttttttctt tatgtcctca
     3721 actctgtcgg tcttctccca tggaagatcc agatcgtctc ttccaaaatg accataggct
     3781 gcagtttgct tgtaaatcgg tcttctcaaa ttgagggtct ggattattgc tgcaggcctt
     3841 aaatcaaaaa gctctgtcac aatactctca atcttctcgt caggaatact accggttcca
     3901 aaggtatcta tccttacaga caccggcctc gcgacaccta ttgcataagc cagctgaacc
     3961 tcacattttt ttgcaagccc tgctgcaaca atattctttg caacatatcg ggcagcatat
     4021 gcagcggaac ggtcaacctt tgtaggatcc ttaccggaaa aagctccgcc cccgtgtctg
     4081 gcatacccgc cgtatgtatc cactattatc ttcctgccgg tgagtccggc gtctccctgt
     4141 ggtcctccaa caacaaacct tcctgttgga tttatgaaaa attttgtctt ttcatcaata
     4201 agatttgcag gtataaccgg ccttattaca tgctccatga tatccttctc aatggtttca
     4261 tggtctacat cggcaccatg ctgtgtggaa atgaccaccg tgtcaatcct taccggcgta
     4321 tccccgtcat actcaacagt aacttgggac ttcccgtcgg gacgaaggta ttctacggtt
     4381 cccttctttc ttacatcggt caaccttctc gtcagcttat gagccaatga aataggcatt
     4441 ggcatcaact caggggtttc atcacatgca aagccgaaca tcatcccctg gtctccagcc
     4501 ccaatagcct caatctgggc atccgtcatc tccccggttt tagcttcaag tgctttgtcc
     4561 acacccattg ctatatcggg ggactgctca tctatagaag tcaaaaccgc acaggtatca
     4621 caatcaaaac catactttgc cctgtcatat cctatttccc tgatggtctt tctaacaacc
     4681 ttgggtatat caacataaca ctttgtcgtt atctcaccgg ttaccaatac cagacctgta
     4741 gtaacagagg tttcacatgc tactcttgcc atgggatcct ctgagtaaat cgcatccaaa
     4801 atcgcatcag atatctgatc acatatcttg tcgggatgtc cctcggtaac agattctgac
     4861 gtaaataatt tttttgtcat tattgtttcc tccttaattt attctgtcag taaaccgcct
     4921 tgaccacata atccatgatg aggttcaaaa acgtagaatt tcgtcttttg ctggccattt
     4981 cactccgtga aatcccacta acaaaaaatc ctcttatgta acaaagagga cctcattaat
     5041 cctcatcttc caggtctatt aacctgtagg aattggcacc gatacaatta agccggttgc
     5101 cgggtttcat agggcccatt ccctccacct ctcttgataa ggctttcact gtattatatt
     5161 gtcatacaat aatgtatcat atacataaaa tttagtcaat aaataaagaa taaatgacca
     5221 aaaagcaatg ggggctcaat gcccccagag tttttgtccg cttcaattat atgcacccca
     5281 aaaacagatt acatcacgca tttgcatata gttcttcaaa ctcatgccct tcaatctttt
     5341 ccttctcaat caatacattt gctacggtat ggagcttgtt tatattctcg tttaatatat
     5401 ttttaatctt ctgataacaa gtatctatta tatgcttaac ctcgcggtct atttgagcag
     5461 ctacctcttc actgtaattt ctggtctggg caaaatccct tccgataaat acttcgtcat
     5521 tctcattgcc gaatatgagg ttgcccagct tatcgctcat accatactta gttatcatgc
     5581 ttctcgctat gctgttagcc tgctttaggt cagagtatgc accggtgctg atttctccga
     5641 gcacaatttc ttccgcagcc cttcctccaa gagaaacaac gattttttca agaagatggg
     5701 acttggtatg atagttttta tcctcatccg gtttgtgggc agtaaagcct cccgccatac
     5761 ctgaaggtat aatagatatc ctgtctacct tgtcggtggt ggatacggct tttacagcga
     5821 tagcgtgacc ggcttcatga tatgccgtaa gccttttctc tttatcgctc ataaccctgc
     5881 tcttcttctc cggacccata acaaccttga acactgcttc ctttatttcc gacattgtta
     5941 tcattgtctt attgttcctt gcagcaagca gtgccgcttc attaagaaga ttttcaaggt
     6001 ctgctcctgt aaatccggga gtactctttg ccagttcatc cagctttaca tcttccgcca
     6061 agggcttgcc cttcgcatga acctttaata tttcttccct tccctttatg tccggtaaac
     6121 cgacaacaac ccttctgtca aaccttccgg gtcttaacag agccgggtca agaatatccg
     6181 gcctgttggt tgcagcaagg attattactc cctcgttgac accaaagccg tccatttcaa
     6241 ccagcaactg attaagggtc tgctcccgtt catcatgacc gcctccgaga cctgctcctc
     6301 tgtgtcttcc caccgcatca atctcgtcta taaagataat acagggtgca ttcttttttg
     6361 cctggtcaaa aaggtctcta acccttgaag caccgactcc gacaaacatc tccacaaaat
     6421 cggaaccgct tatgctgaaa aacggaacac cggcttctcc cgataccgcc tttgccagca
     6481 aggttttacc cgtaccggga ggacccacca gaagtactcc tttcggtatc cttgctccca
     6541 attccaagaa tttcttcgag tgctttaaaa attcaacgat ttccttcaat tcctcttttt
     6601 cttcatctgc ccctgcaaca tcgttaaaag ttaccttctt cttgtcatcg atggtcattt
     6661 ttgccctgct cttaccaaag gacattaccc tgtttccgcc gcctccctgg gactgctgca
     6721 ggaagaatac ccagaacagc acaaaaatca atatgactgc cagagtgggt ataatcgaaa
     6781 gccaccatgg cggtgttgga ggcggtgcag aatccactat caaactttcc tctacaataa
     6841 aactgttaat ctcattcata aaagtcggag tgtccggtat atagacatac tgttccttca
     6901 cctgtttgtc tttgtacttt accgtggctt tatctccctc aagtacaact tcctgaacct
     6961 ttccatcata tatatccctt ataaggtcgg agtactttcc ttttttcgga tccggaccga
     7021 ttcttattac aaccaaaaaa gctaatagca taacaaacaa tactatatag aagcttatgt
     7081 tcctaaaata tttcaaatag cttccctcct taattaatac actgatagta cactgaaatc
     7141 tatttttgat tcgatttgtt cttaaatatt tattacatta tattaacata acgatgtata
     7201 aaacacaaga aacctttagt aattaaaaag ttaataaatt gttaactttc cgtaccgtct
     7261 ttataaaccc acggtttcaa tacgcaaaca tcggcaaggt ttcggtattt gccgccataa
     7321 tccagcccat accctaccac aaacttatcc ggtatcgtta ttccccggta ttcaatttct
     7381 atttcaactt ttcttcttga tggcttatcc agggcagtac atatttttac gtctttaggt
     7441 ttccgggctt tgaacaaatc cttcaaataa tgaagagtaa gccccgtatc caccaaatct
     7501 tcaacaatca gtacgtgttt gcctgtcaca tcaatatcgg tatccttaat tattctgaca
     7561 accccggatg attttgtgga attgccatag cttgacaccg atataaaatc tatctcaata
     7621 ggtatggtta tttccctgat gaggtccgaa aaaaatacca ctcctccttt taacacaccg
     7681 atgaggacaa gttccctacc ctcataatcc gaagatatcc gctttcccaa ttcccttgca
     7741 ttgtttttaa gttcctctct gccaatcagt atctcttcaa tttcctcttt catttcttcc
     7801 tcccaattaa cattgatttt aagtaccgtt gccttgcagc catttataaa ttcattctct
     7861 tgattctatc acaacctgtt ttataatacg caggattaat tatccgtgtt tgtctcccgc
     7921 ttaaaatata acttcaatat ggttttagta ttttcagtta ctttaaattt atcacttatt
     7981 ctataaccta ttatccatac aatttcttta tcctttgaaa taagcggtat ctgatccctg
     8041 agatgtcttt gaaccttgct gtctataaaa aactccttga gcttttttgt tccggttgac
     8101 cctagcggtt taaaaacatc accatctctg cggtacctta tattaatacc atcttttagc
     8161 ctgtcataat cgaaaaattg aaccaagcta tcgccttttg tttttatata gtctttaacg
     8221 ccctcgggca catcaataac tatagcttcg acagagctgt ttatctcatc aatacaggtg
     8281 atccccggta tgacaaccct tttttcaaaa tcccggacac agcactcctt ctcacacaaa
     8341 tatattttta gcgtttcgta ggattttaaa gcccttaatc ctccgggaag atgcagcata
     8401 gcccctgtcc ttccgttttc aatgagatcc gctacactgt ccacatgtac gttttcaatt
     8461 cccctcaaat tccctctgat ttcttttatt aaattgcgga caaccctgcg aaccacagct
     8521 atatgatacc ctcttatttc ttttatgttc aaggttgcct cgttattttt tatttcaata
     8581 tttatttcat tatatactt
//
LOCUS       DAOOJC010000045         8364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_7340_length_8364_cov_4.005657, whole genome shotgun sequence.
ACCESSION   DAOOJC010000045 DAOOJC010000000
VERSION     DAOOJC010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8364
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7340_length_8364_cov_4.005657"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLA01_02255"
     CDS             <1..580
                     /locus_tag="PLA01_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPD00154.1"
                     /translation="RIIMFDNDIAKDFGTHLNKLLDQGVKGLIIDLRDNPGGDYDQVA
                     AIADRLLPEGLIVYTEDRIGNRAEKKSDSTELNIPLSILVNGNSASASEVLSGAVKDY
                     GKGKLIGTKTFGKGLVQAVINLEDGAGIKVTIARYFTPSGVCIHEKGIEPDIVVELDE
                     KYEYYPVSQVPREDDVQLKKAIEVIKEQLGEN"
     gene            660..848
                     /locus_tag="PLA01_02260"
     CDS             660..848
                     /locus_tag="PLA01_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HPD00155.1"
                     /translation="MENLDEKIKTEIITELGLFDKVKKHGWKSLTAKETGRIGGLITK
                     RKKLMQMEKQDRVAGQNL"
     gene            1084..2070
                     /locus_tag="PLA01_02265"
     CDS             1084..2070
                     /locus_tag="PLA01_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPD00156.1"
                     /translation="MDKGTINWGIMGCGSIAREFAHALKAVPGANLIAAASKSGKSRG
                     FAEEFDLPYYYDNYDDFVKNEKVDVVYIATTHNFHYENVMLCLNNDKHVLCEKPFALN
                     TKQALRLIETARAKRLFLMEAMWTRFSPALSRLVSIVNDGLIGEIKILKGQFCIHVPF
                     DPGHRLYNPHLAGGALLDLGIYPIAFACMVLKKYPVKITGTLNIGKTGVDEESHYLLK
                     FDKGETALLSSACTFSAPINFSIYGTKGHILVPDFYFPSQLIIKLNDRDEEHIDVPYE
                     TTGKNYEAMEAMDCLRQNKTESDKQTFYDTIKIMEIMDHLRGNDLIYPCELN"
     gene            2171..3010
                     /locus_tag="PLA01_02270"
     CDS             2171..3010
                     /locus_tag="PLA01_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00157.1"
                     /translation="MERWQQFEIEACEYLNSVFKDLPFTFKCTGGSNSLAEDIMVYKG
                     KDHIFSIEAKLSPAQGGQFSVIPIESDGKIEYTYSRDNKFSLDGNSESIMKHINQNIG
                     KYSHVTQRGIDIDGIQPVLINWVIGHYKKKRSKFIITSKGIGSFKAIVPTDELKDNFY
                     VHACLRRKRSGPRKVPNYQYELAESLVKRHFGGLGFGIKGFEYDGKGISLTLNSGAKL
                     SKDQRYLPENMYISSDKYDRNRFTIKKRSSTNNINVVFSLEYKYNTSTGLELLRNELL
                     KYS"
     gene            3441..4025
                     /locus_tag="PLA01_02275"
     CDS             3441..4025
                     /locus_tag="PLA01_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPD00158.1"
                     /translation="MLEVPDKKIINLCKKNNREGFELLFKKYERYIYSICYSYTYSQQ
                     DALDLVQEIFLRIYRGFAKVNDKKPLFPWIKRIAVNTCINYKRDNRNRASELSIHATI
                     DDGKATLEDMVYDNGLTENEVMFMDTKKVLEESIRELPNEVKMAVLLRHIKGLSYNEI
                     SELMECPVGTVKTYIYRGRKLLKDSLVKKGVWEV"
     gene            4029..4463
                     /locus_tag="PLA01_02280"
     CDS             4029..4463
                     /locus_tag="PLA01_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPD00159.1"
                     /translation="MECGLGSYMIQMYIEGTIDPLEKIFVEEHLKVCKNCRKELTRLK
                     LLYFELDNLDEPSEVPDELEQVRNMVLDSIFDTGNKYGVKKFIKQQKKALSLASGFAD
                     FIPGRSIVKKGIKATGSTIGRVSLKSAKYGLKYGLKLIQERT"
     gene            4467..5126
                     /locus_tag="PLA01_02285"
     CDS             4467..5126
                     /locus_tag="PLA01_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HPD00160.1"
                     /translation="MKLLLIFGYVIMALILLLVLIMVVPVEFKIHGEKFEKTFLRARV
                     IWFKGLLGFHFRLADTEKMQMYVSVFGLKKKIDAHLFNKFKGDKEKPERKEKDKRKKY
                     SRNYIESDFLRCALISIKKVLRHIKPRKFTIEGRVGFEDPYVTGLVCAVLNMFYYELK
                     KANINVNTVFDDEVLEGRCLIQGRVVLAYMAYIALRLYFSRPVRIKQDQKFEEVKSYV
                     N"
     gene            5116..5502
                     /locus_tag="PLA01_02290"
     CDS             5116..5502
                     /locus_tag="PLA01_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HPD00161.1"
                     /translation="MSIDSKFDVNENVSVLFEKLEKFLTSKTVIGEPIKVGEATLIPF
                     ITTSFGLGSGGGDGYDAKGAHGIGGGSGIGARISPTAVLVIRGDDIEMIPIKKSAGFE
                     KLVEMVPEIVSKVNCCKKGDGMNKEE"
     gene            5825..6562
                     /locus_tag="PLA01_02295"
     CDS             5825..6562
                     /locus_tag="PLA01_02295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014522645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD00162.1"
                     /translation="MYSGFAYVYDKLMYDVNYKEWADYIEKIFEVNNLRPSLLLDLGC
                     GTGSFCLEMDKRGYDMIGIDSSEDMLSCAKDKSIDRDILYLNQDMTGFELYGTVDAIV
                     CLMDSINYLIYKKDIKKVFGLVKNYLNPGGLFIFDINTPYKFENIFKDNVFYDVSEDV
                     SYIWQNSFDRKKRLCEFDLTFFIKDGQYYNRYDEIHYERCYEIEELKSMVIGSGLELL
                     NIYDSLRLCSPSKKSQRVFFVCRKGGK"
     gene            6562..6867
                     /locus_tag="PLA01_02300"
     CDS             6562..6867
                     /locus_tag="PLA01_02300"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPD00163.1"
                     /translation="MSFFEDVYEVVRKIPKGKVATYGQIAKILGYPRKAKIVGWALHK
                     NPYYGEVPCHRVVNRNGEISSGFAFGGEHAQQKLLEQEGIIFDESGKINLKKYLWNP"
     gene            7046..7246
                     /locus_tag="PLA01_02305"
     CDS             7046..7246
                     /locus_tag="PLA01_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPD00164.1"
                     /translation="MEKGRVKWFNAEKGFGFIEREGGNDVFVHFSAINMDGYKTLEEG
                     AEVQFEVVEGAKGPQASNVTKA"
     gene            7407..8273
                     /gene="hslO"
                     /locus_tag="PLA01_02310"
     CDS             7407..8273
                     /gene="hslO"
                     /locus_tag="PLA01_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HPD00165.1"
                     /translation="MEDYIVRATAGNGTIRAIAAVTTNMVKESQNTHGLSPLATAALG
                     RTMTAAAIMSTMLKGEGDTITVQIKGDGPIGGIVVVSDSQANVKGYVHHPLVYLPLNS
                     QGKFDVAGAVGKGYLNVIKDMGLKEPYIGYVDLVSGEIAEDITYYYAFSEQIPTVTSL
                     GVLTNASEIVASAGGFILQLMPGADEETIAFIENKVNSIPSVTGLLSEGKLPEDILNI
                     LLSEKDLKIIDKKPCRYMCNCSRDRMERSIISLGKKEILDIVNTDHGAELQCHFCNKK
                     YRFSEEDLLSLI"
BASE COUNT         2796 a         1186 c         1940 g         2442 t
ORIGIN      
        1 aagaataata atgttcgaca acgacattgc aaaggatttc ggaacccact taaacaagct
       61 tctggatcag ggagtaaaag gattgataat tgatttgaga gataatccgg gcggcgatta
      121 tgaccaagtt gcagcaattg cagacaggtt gttgccggag ggtttgattg tatatactga
      181 agaccgtatc ggtaatagag cagaaaagaa atcggattca acggaattaa acattccgct
      241 gagtattttg gttaacggta acagtgccag tgcttccgag gtgctgtccg gtgcagttaa
      301 ggattacggt aaaggcaaat taatcggtac aaagaccttt ggaaaaggct tggttcaggc
      361 ggtaataaat cttgaggatg gagcaggcat aaaggtgact atagcaaggt attttactcc
      421 ctccggagtg tgcatacacg aaaaaggtat tgaaccagat atagtagtcg aactggatga
      481 gaagtatgaa tactatcctg tatcccaggt gccgcgggaa gatgacgtac agctgaagaa
      541 ggcaattgaa gtaataaagg agcaattggg agaaaattga cattaaaata atatttttgc
      601 tcaaaatatg tataccccta tgtggttttg tacataataa ccacaggagg tgggacgaag
      661 tggaaaactt agatgaaaaa attaagactg agataattac cgagctcgga ttgtttgaca
      721 aggtaaagaa gcatggatgg aagagtttaa ctgccaaaga gacaggacgg atcggaggtc
      781 ttattaccaa gaggaagaaa ctgatgcaga tggaaaagca ggatcgagtt gccggacaaa
      841 acctatgaca taatagtgat tcagtacatt gcaatagtcc tcaaacatat tagataatag
      901 ctttttgttg tgttgacata aaacaaaatg cttggattta caaaacatcc ggatattccg
      961 tggagagtat ccggatgttt ttttacaacc ctgaaactag tttaatatgg aattgcatca
     1021 tgaaatatgg taatatactt atataaaaaa agaaaagtaa ttttcggtgt ttgtggggat
     1081 taaatggata agggtacaat taactgggga ataatgggct gcggtagtat tgcaagggaa
     1141 tttgcacatg cactgaaagc tgttccgggt gccaacttaa tagctgcagc ttcaaaatcg
     1201 gggaaatcaa ggggattcgc agaggagttt gatttaccgt actattatga caattatgat
     1261 gattttgtaa aaaatgaaaa ggtcgatgtg gtgtatattg ctacgactca taattttcac
     1321 tatgaaaatg ttatgctgtg tctgaataat gataagcatg tactttgtga aaagcctttt
     1381 gcattaaaca caaagcaggc tttgcggctt attgaaacag ccagggctaa acggctgttt
     1441 ttaatggagg ccatgtggac aagattttca ccggccctgt ccagattggt aagcattgta
     1501 aatgacggtt tgataggcga aataaaaata ctgaagggac aattctgtat ccatgttcct
     1561 ttcgaccccg ggcacaggct gtataatcct cacctggcag gaggagctct tttggatttg
     1621 gggatatatc ctatagcctt tgcatgtatg gttctaaaga agtatcctgt taaaataacc
     1681 gggaccctga atatagggaa aactggtgtg gatgaagaat cccactacct cttgaaattt
     1741 gataaaggtg aaacggcgtt gctttcctcc gcttgtactt tttctgcacc gataaatttt
     1801 tcgatttacg gtaccaaggg gcatattctt gtaccggatt tttattttcc ttcacagcta
     1861 atcataaagc ttaatgatag ggatgaagaa catattgatg ttccttacga aaccaccgga
     1921 aagaattatg aggcgatgga agctatggat tgccttcggc agaataagac agagagtgat
     1981 aaacagacat tttatgatac aataaagata atggaaatca tggaccattt gaggggaaat
     2041 gatttgatat atccctgcga attgaattga tattttggct ggagacagat tatcgcatgg
     2101 ataaacaacg ttatttagaa cccaaattat tattttacat atttttatta accatgaaag
     2161 aggtgagtat atggaacggt ggcagcaatt tgaaattgaa gcctgtgaat acttaaacag
     2221 tgtatttaaa gatttgccct ttactttcaa atgtactggc ggatccaatt cattagccga
     2281 ggatattatg gtctataaag gaaaagacca tatattttcc attgaagcaa aattatcccc
     2341 ggcacaaggc ggtcagttct ctgttattcc gatagagtcc gacggaaaga ttgaatatac
     2401 ttattcaagg gataacaaat tttctctgga tggtaattcg gaaagcatta tgaaacatat
     2461 aaatcaaaat atcggcaagt attcccatgt tactcagagg ggtattgata ttgacggtat
     2521 acagccggta ttaataaatt gggtcattgg tcattacaag aagaagaggt ccaagtttat
     2581 cataacttca aaagggatcg gcagttttaa agcaattgta ccgactgatg agttaaagga
     2641 taatttttat gtccatgctt gccttcgaag gaagaggagc ggtccgagaa aggtgccgaa
     2701 ttaccaatac gaactggctg aatcgttggt aaaaagacat tttggaggat tagggtttgg
     2761 aataaaagga ttcgaatacg atgggaaggg aatttcattg acactgaact ccggtgccaa
     2821 attatcaaaa gaccagcgtt atttgcctga aaatatgtat atttcgtccg acaagtatga
     2881 cagaaacaga tttacaatca aaaaaagaag ttcaaccaat aacattaatg ttgtgttcag
     2941 tctggaatac aaatacaata catcaaccgg acttgaatta ttaagaaatg aactgttaaa
     3001 gtatagttag cagagaatgg gatacaggtt gtttgcattt tataatcttc ttttataaat
     3061 tatgaatata tgaaaggata aggtatatgc cagtttactt gtaatggata aatgaactca
     3121 cacattaata cctgtaattg caaaacacat aaggtgttta gttcaacagg tgtagtgtgt
     3181 cttttattac cattacaagg aacgcattaa ccgttaacag caatgcggaa actcttggag
     3241 ggtttccgtt ttcttttata tttagatttc gcaatgtgac atctttcata atcaaaagaa
     3301 ttaattccac ttaaaagagt taataaaaag gatacggtat tgaaattatg ttatattaat
     3361 tttaaattat caaaactttt ttatttttta tgtgtatata ttaataaatg cagctgaagc
     3421 ttatatgaag ggcggttaac ttgttggagg ttccggataa gaaaataata aatctatgca
     3481 aaaagaataa tcgggaaggc tttgaacttc tgtttaaaaa atatgagagg tacatataca
     3541 gcatttgtta cagctatacc tattctcagc aggatgccct tgatctggtg caggagatat
     3601 ttttgaggat ttacagggga tttgcaaagg ttaatgataa aaaacctttg ttcccctgga
     3661 taaaaagaat tgcagtgaac acctgtatta attataaaag ggataacagg aacagggcat
     3721 cggaattgtc gattcatgca accattgatg atggcaaggc aactcttgag gatatggttt
     3781 atgataacgg acttaccgaa aatgaggtta tgtttatgga taccaagaag gttttggaag
     3841 aatctatcag ggaacttccg aatgaagtaa aaatggctgt tttattgcgg cacatcaaag
     3901 gacttagcta taatgagatc tctgagctta tggaatgccc tgtagggact gttaagacat
     3961 acatttaccg gggcagaaaa ctcttaaagg acagtctagt aaaaaaaggc gtatgggagg
     4021 tgtagagtat ggaatgcggt ttaggcagct atatgataca gatgtatata gaaggaacaa
     4081 ttgatcccct tgagaaaatt tttgttgagg aacaccttaa ggtttgcaaa aattgcagga
     4141 aagagcttac ccgtttaaag cttctttact ttgagcttga caaccttgat gaaccgtcgg
     4201 aagttcccga cgaattggag caagtcagga atatggtgct cgatagtatt tttgataccg
     4261 gcaataaata cggtgtaaag aagtttatta agcagcaaaa gaaagcccta agtctggctt
     4321 cgggttttgc ggattttatt cccggaagaa gcattgttaa gaagggtata aaggccacag
     4381 gttcaacaat aggaagagtg tctttgaaga gtgccaaata tgggcttaaa tatggattaa
     4441 aactgattca ggagaggaca tagaggatga agttattgtt gattttcgga tatgtaataa
     4501 tggctttgat tttgttactg gtattaatta tggttgtgcc ggttgagttc aaaatccatg
     4561 gagagaaatt cgaaaagaca ttcttaagag ccagggtgat atggtttaaa ggtttattgg
     4621 gttttcactt cagattggca gataccgaaa aaatgcaaat gtacgtatcg gtttttggat
     4681 taaaaaagaa aatagatgcc catttattta ataaattcaa gggtgacaaa gagaaaccgg
     4741 aaaggaagga aaaggataaa aggaaaaaat actcccggaa ttatattgaa tcggattttc
     4801 tgagatgtgc ccttataagt attaaaaagg tgcttaggca tataaaaccc cggaaattta
     4861 caatcgaagg cagagtgggg tttgaagatc cttatgtaac tggtttggtt tgtgctgttt
     4921 taaatatgtt ttattacgag cttaaaaaag caaacatcaa tgttaatacg gtgtttgatg
     4981 atgaagtgtt agaaggcagg tgtctaatcc agggaagagt agtattggct tacatggcgt
     5041 atatagcttt aaggctgtac ttctcgagac ctgttagaat aaagcaagac caaaaattcg
     5101 aggaggtaaa atcatatgtc aattgattca aaatttgatg ttaatgaaaa tgtcagtgta
     5161 ttattcgaaa aattggagaa gttcctgact tccaaaacag taataggcga gccaatcaaa
     5221 gtcggagagg ctactttgat accgtttatt actacaagct ttggactggg atcaggcgga
     5281 ggagacggtt atgatgccaa aggtgctcac ggtattggag gaggatcggg tatcggagca
     5341 agaatatcac ctacggcagt acttgtaatt agaggtgatg acattgaaat gattcccata
     5401 aagaagtcgg caggtttcga aaagcttgtt gagatggtgc cggaaatagt ttcaaaagtt
     5461 aattgctgca aaaaaggtga tgggatgaac aaagaagagt aagacgcgtg aaataaaagt
     5521 gaatatagta ttccaaaaac ttcaagttgc ttcacaatgt ttcttccata ttttccttca
     5581 ttgtaagtga gatttcacga agtgaaatga ccaaaaatcg aaagggctcg atgctctttc
     5641 gattttttct tttcaatgta cccatgtttt tttgctgcgt tgcagatgca agcacggtgt
     5701 gttataatta actcgctttc tatgaagttt cttttttaaa aagctgattg cattattatg
     5761 aagcgaactt tgtggagaac ctattggaga tactgtactt tatgcttttg gtggggtggt
     5821 taatatatat tccggttttg cttacgtata tgacaagctg atgtatgacg tcaactacaa
     5881 agagtgggcg gattatatcg aaaagatttt cgaagtgaat aatctaaggc cgtcattatt
     5941 attggacctt ggctgcggta ccggcagctt ttgccttgaa atggacaaaa gaggctatga
     6001 tatgattggc attgattcat cagaggatat gttaagctgt gcgaaggata agtctattga
     6061 ccgagatatt ctttatctta atcaggacat gaccgggttt gagttgtatg gaacagtgga
     6121 tgcaatagtt tgtcttatgg acagcataaa ctacctgatt tataaaaaag atataaaaaa
     6181 ggttttcggt cttgtgaaga attatttaaa tcccggtgga ttgtttatat ttgatatcaa
     6241 cacgccttat aaatttgaga atatttttaa agataatgtg ttttatgatg tttccgaaga
     6301 tgtttcttat atatggcaaa actcttttga caggaaaaaa agactgtgtg agtttgacct
     6361 gacttttttt ataaaagacg gacaatatta caacaggtat gatgaaattc attatgagag
     6421 atgctatgag atagaagaat taaaaagcat ggtgattgga agcgggttgg aattattgaa
     6481 tatttatgac agcttgaggc tgtgttcacc gtctaaaaaa agtcagaggg tcttttttgt
     6541 ttgcagaaaa ggaggcaaat gatgtcgttt tttgaagatg tgtatgaagt agtgagaaag
     6601 attcccaagg ggaaagttgc tacttacgga caaattgcca aaattctggg atatccgcga
     6661 aaggctaaaa tagttggatg ggcattgcat aaaaatccat attatggcga agttccttgc
     6721 cacagagttg taaacaggaa tggagaaatt agttccgggt ttgcttttgg aggcgaacat
     6781 gcacaacaaa aattgcttga acaggagggc ataatttttg atgaaagcgg aaaaataaac
     6841 ttgaaaaaat atttgtggaa cccctagaaa ctccattgta ttggcgatga tgataattgg
     6901 ggatgaagat aatatataat tatcgtaaaa atgtaaatat cagattgaca agcagtaaag
     6961 caatgtgata aactaatata gatttagtaa aagcatcgaa gatatgcaag ctaagttaaa
     7021 gtatcaagtt caggaggagt gcaggatgga aaaaggaaga gtaaaatggt ttaatgctga
     7081 aaagggtttc ggttttattg agagagaagg cggaaacgac gtttttgtac atttttcagc
     7141 aataaacatg gacggctata agacattgga agaaggtgct gaagtccaat ttgaagttgt
     7201 tgaaggtgca aaaggacctc aagcttcaaa cgtgacaaag gcctaattta gtctaaaaga
     7261 tttaacataa aatggccaca tgccccggta tttatatacc ggggttttca atatggagaa
     7321 ttattatatg aaatgaccta ttttattctt ttcactttgg tgaaatttgt ttgtaaatga
     7381 agcatttatg aaggtgggag taattgatgg aagattacat tgtcagagct acggcaggga
     7441 atgggactat aagggctata gcagcggtta cgacgaatat ggtcaaggaa tcgcaaaata
     7501 cacatggctt gtctccttta gcaacagcgg cattgggcag aacaatgact gcagcagcaa
     7561 tcatgtctac tatgctaaaa ggagaagggg atactatcac ggttcaaata aagggagatg
     7621 gtcctatagg agggattgtg gttgtttccg attcacaggc caatgtgaag gggtatgtcc
     7681 atcatccatt ggtttatttg cctctgaaca gccaaggcaa attcgacgtt gccggtgcag
     7741 ttgggaaagg ttacctcaac gtaataaagg acatgggatt aaaagaaccg tatatcggat
     7801 atgttgatct tgtttcgggt gaaattgctg aagacataac ttattattat gcattttcgg
     7861 aacagattcc gacggttact tcactggggg tcttgaccaa tgcaagcgaa attgtggcca
     7921 gtgcaggcgg atttattctt cagttgatgc cgggggcaga tgaagagaca atagcattta
     7981 ttgaaaataa agtaaactcc ataccttctg ttaccggact gttgtccgaa ggcaaattgc
     8041 ccgaggatat attaaacata cttctttcgg aaaaggattt aaaaataatc gataagaagc
     8101 catgcaggta catgtgcaat tgttctagag atagaatgga gagaagcatt atcagtctgg
     8161 gtaagaaaga aatattggac atagttaata cagatcatgg ggcagaactg cagtgccatt
     8221 tctgcaataa aaagtatcgt ttttctgagg aagacttgtt aagcttgata tagataaagc
     8281 cgggaaatag aatgaatttc aaaagaatct ttgtattatt gagtatgaac aagaataatt
     8341 gagagattaa ataataattt taat
//
LOCUS       DAOOJC010000046         8170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_7522_length_8170_cov_3.570548, whole genome shotgun sequence.
ACCESSION   DAOOJC010000046 DAOOJC010000000
VERSION     DAOOJC010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 8170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8170
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7522_length_8170_cov_3.570548"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..2682)
                     /locus_tag="PLA01_02315"
     CDS             complement(85..2682)
                     /locus_tag="PLA01_02315"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HPD00166.1"
                     /translation="MKHYFEEIKAVLKSQNSTKSGIDSEQFQERLEKYGKNKLDEGKK
                     KSIIARFLEQLKDPMVIVLIAAAVISGAVKEIADSVIILLVVILNSILGVVQEGKAEK
                     AIEALQKMASPYSKVRRNGRVAQIKSEEIVPGDIVLLEAGDAVPADMRLIEASSLKIE
                     EASLTGESVPTEKNAAVIAAKDKDVPLGDRHNMAYMGTNVVYGRGEGVVVSTGMQTEM
                     GKIANIISSTGEEKTPLQKKLAGLSKVLSIAVLGICVFIFVFSVLKNGGFSGGHVFDT
                     FLLAISLAVAAIPEGLVAVVTVVLSIGVTKMSKRNSIIRRLTAVETLGCTQIICSDKT
                     GTLTQNKMTVVDSYGDKQKLAISMALCNDASLSGEKGEVVGEPTESALVRYAFEMGLD
                     KNELEAQMPRIAEAPFDSMRKMMSTVHKIQNGKYLQYTKGAPDELLKKCTHIHTEDGV
                     VPLTDKHRSQILGENKKMADKALRVLASAMKETDSLPSDISPDSLEQGLTFIGLAGMI
                     DPVRPEVKAAIEKCRNAGIRPIMITGDHKDTAVAIAKELGIITDESQAITGAELSEIS
                     DSDFEKKINSFFVYARVQPEHKVRIVNTWKKLGKITAMTGDGVNDAPALKSADIGVGM
                     GITGTDVSKNVSDMVLADDNFASIVYAVEEGRRIYDNILKSIQFLLSSNLSEVVALFI
                     ATLSNFTLFSPIHILWINLVTDTFPAISLGMEEAEEDIMRKPPRNAEEGVFSNGLGLD
                     ILYQGIIIAILTVISFFAGRRHSQVMGMTMAFLTLSSCEVFHSLNLRSRTKSVFRLKS
                     HNKFLLGAMVLSFVLSIAVIYIPGINNIFELTPLSAGNFLMAIAIAFIIIPVVEICKL
                     IRTLFLKQK"
     gene            3067..3828
                     /gene="sigI"
                     /locus_tag="PLA01_02320"
     CDS             3067..3828
                     /gene="sigI"
                     /locus_tag="PLA01_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-I factor"
                     /protein_id="HPD00167.1"
                     /translation="MDWRSQNTNQKREQTKKIISEYLDKISLGDSAVREEFILKFKPF
                     ILKQVFKATDKFAEPENSEEYSVALFAFNEAIDTYDEKRHPNFLVFCEQVIRRRLIDY
                     KRKNKKNSMVFPFSYFESEDNRLENTILQTDTDDGIQRLEFLEDMDHFKDELACFNIS
                     FRDLVLCTPKHRDSRESLINVARTLAGNDELYGKLKRKKKIPTIELLKLTSVSRRTIE
                     RNKKYIIAVSLILRSNLDIFKEYAECCKERRADMR"
     gene            3825..6116
                     /locus_tag="PLA01_02325"
     CDS             3825..6116
                     /locus_tag="PLA01_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HPD00168.1"
                     /translation="MITGKVIDMDKKTAVVMTDDFAFINVKVRPEMAVGYKFEFGELD
                     IVKSKPRLKAYIPIAACFAILLSLAIMFMHGSIGPKGIYAFLAVDINPSIEFSIDYNN
                     RVVGVRNLNKDARTVTDNLELVGKTVDEALRDVIQRSKECGFIVPERENIVLVSVARN
                     KQDQGKSDNEKLKGNIDRLTAHVNDVLSALDDSNIETKVLKITDEQRATSLEKDISMG
                     RYVIYLKAIERNMDLTIDKMRDVNLSQILKYISIGYGEGDIPVASPSAAHTPSGEQSP
                     TPDETSAPVSTATSVPAATEGLVDSTPSHGQPTPVRTATYDAKETGTMLVKTPTPKAV
                     ATVAVKTPTPKAAATVAVKTPAPKATATPPVKTPTPKATPRKTSSGSVWVDNSNAKID
                     EIRKRDIKITVLDTNRRELEGVTVEVKQTRHAFAFGTALRRSGLDNPSYVEFVKKYFN
                     WAVFENESKWYTNEPTMGNVTYEEADYMYDFCQRNGITVRGHCIFWEDQGNQPSWLGW
                     LDPFSLRFAVDNRLNSVVSHFKGKFVHWDVNNEMLHGSFFKDRLGESIWPYMFNRTRE
                     IDPNVKIFVNNNVTTVKEIDDYLAQINWLKSQGVKIDGIGLHGHFGEKVDRDVVKGML
                     DKLSSANIPIWITEYDSCTSDENRRADNLENLYRTAFSHPSVQGILMWGFWEGAHWKG
                     RDAAIVNYDWTLNQAGKRFEALMEEWTTKESGRTDETGSFYLRGFYGEYEITLTVPGK
                     GKTVRRLTLSPGKGALQQTFVID"
     gene            complement(6267..>8170)
                     /locus_tag="PLA01_02330"
     CDS             complement(6267..>8170)
                     /locus_tag="PLA01_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPD00169.1"
                     /translation="GGGSGIGYIENGDYVVYNNVDFGNGATSFKAVAASGASTTTNIQ
                     LRAGSPSGTLLGTLAVQSTGGWNEYKEHTCSLNNSIKGVANLYLVFSGAVNLDSFTFT
                     SGGSNPTPTPASTQRPTQNPDTGEKFLGQIIASNIPSNFAQYWNQVTPENSTKWGSVE
                     SSRDVMNWSQANMAYNYAKSNGFPFKFHTLVWGSQEPGWVSGLSASEQKAEVLEWFDA
                     AAEKFGDSEYVDVVNEPLHAKPSYRNAIGGDGSTGWDWVIWSFEEARKRFSGKLLINE
                     YGIINDGNATNQYIQIINLLKERGLVDGIGIQCHCFNIDTVSVNTMKSNLDKLAATGL
                     PIYVSELDITGDDSTQLQRYQEKFPVLWEYPSVKGVTLWGWIQGQTWKDNTHLITSSG
                     TERPAMVWLKQYLGNSSSPTPTTKPTPTPTRTPDNTQRDAYSKLEAENFSDLNSSTIE
                     IIGNGKGGEGIGYIESGDYVVYNNVDFGKDGALSFKAMVASAAETTTNIQIRAGSPSG
                     TLLGTLAVQSTGDWDSYEEQQCNITNKVTGVKNLYLVFTGPVNFDWFTFVPMSSTDII
                     YGDLNDDKSVDSTDVTMMKRFLLKKLDLSETARTNADVNKDGSVDSTDLTLIKRYVLK
                     KIDSFKDFQ"
BASE COUNT         2355 a         1632 c         1758 g         2425 t
ORIGIN      
        1 acggtggtgt gggaggtcgg taaataaaat aattatttac ctcctacccg attaaatatg
       61 atgaacaatg ttaattcaat ggttttactt ctgcttcaaa aacaaagttc ttataagctt
      121 gcagatttct acgacaggaa taataataaa tgcaatagca atagccatta agaaatttcc
      181 ggcggaaaga ggagtaagct caaatatgtt gttgattccc ggtatgtaaa taacagctat
      241 cgacaacacg aaggacagta ccattgcacc cagtaaaaac ttgttatggc tttttaatct
      301 gaaaaccgat tttgtccttg aacgcaggtt caaagaatgg aacacctcac aagaggatag
      361 agttaaaaat gccattgtca ttcccattac ctgtgaatgt cttctgcctg caaagaatga
      421 tattactgtc agaatggcaa ttataatgcc ctgatacaat atatccaaac ccaatccatt
      481 ggagaaaacg ccttcttctg cattgcgggg cggttttctc ataatatcct cttcggcttc
      541 ctccatccca agggatatcg cgggaaatgt gtctgttaca aggttaatcc acagaatatg
      601 aattggtgaa aacagggtga agttactgag ggtagctata aacagtgcca caacttcgct
      661 taagtttgac gacagaagaa attgtattga tttgagaatg ttatcatata ttcttcggcc
      721 ttcttcaacg gcataaacaa tagatgcaaa attatcatcg gcaagtacca tgtccgatac
      781 attttttgaa acatctgtgc cggtaattcc catacccaca cctatatctg cggacttaag
      841 agcaggtgca tcgttaactc cgtctccggt cattgcagtg attttaccga gctttttcca
      901 tgtgttgaca atacgaacct tgtgctcagg ctgtacacgg gcgtatacaa agaaactgtt
      961 tatctttttt tcaaaatcgc tgtccgatat ttcggaaagc tctgctcctg taatagcctg
     1021 gctttcatcg gttataatcc ctaattcctt tgcaatggct actgcggtgt ctttatggtc
     1081 gcctgtaatc atgattggac gaatgcctgc attccggcat ttttcaatag ctgctttcac
     1141 ctcagggcgt acagggtcaa tcatgcctgc cagccctatg aatgtaaggc cttgctcaag
     1201 actgtcggga gaaatatctg atggaagaga atctgtctct ttcatggctg aagccaggac
     1261 acgcaaggct ttatccgcca ttttcttgtt ttccccaagt atttgggagc ggtgcttgtc
     1321 ggttagaggg acaactccgt cctccgtatg tatatgggtg cattttttca acagctcatc
     1381 cggtgctccc tttgtatact gaaggtattt tccgttttga attttatgga ctgtggacat
     1441 catttttctc atggagtcaa aaggggcttc tgcaatacgg ggcatttgag cctccaattc
     1501 gtttttatcc aagcccattt caaaggcata acgaaccagg gcactttctg ttggttctcc
     1561 caccacttca cccttttccc ccgacaggga agcatcattg cacagtgcca ttgatatggc
     1621 gagcttctgc ttgtcgccat aggagtcaac cacggtcatt ttgttttgag tcagtgtacc
     1681 ggtcttatcg gaacagatga tttgagtaca tcccaaggtt tcaactgcag taagtctccg
     1741 aatgatagaa ttccgctttg acattttggt tacaccgata gaaagcacaa cggtgacaac
     1801 tgccactaat ccttcgggga tagctgctac tgcgagactg attgccaata aaaacgtatc
     1861 gaaaacatga cctcctgaaa aaccaccgtt ttttaataca ctgaaaacaa atatgaaaac
     1921 acaaattcca agaaccgcta tgctaaggac tttgcttaat ccggccaact ttttctgaag
     1981 cggagttttc tcctcgcctg tgctggaaat aatatttgcg attttcccca tttctgtttg
     2041 cattccggta ctgactacaa caccttctcc gcggccgtat acgacattcg ttcccatata
     2101 agccatgttg tgacggtcgc ccagaggaac gtccttgtcc tttgcagcta tgacagcagc
     2161 attcttttca gtgggtaccg actcacctgt gagtgaggct tcctcgattt tcaggcttga
     2221 agcttcaatc agcctcatgt ctgccggaac ggcatcccct gcttcaagaa gaacgatatc
     2281 accgggtaca atttcttccg atttgatttg agcaacccta ccgtttcgcc ggacttttga
     2341 gtaaggtgag gccattttct gcagtgcttc gatggctttt tcagccttac cttcctgaac
     2401 aacacctaat attgagttta gaataaccac taacagtata atgacagaat cggctatttc
     2461 cttaacggca ccggatataa cggcagctgc tatgagaaca ataaccatgg ggtctttcaa
     2521 ttgttccaga aatcttgcaa taattgattt ttttttgccc tcatccagtt tgttttttcc
     2581 gtatttctcc agccgctctt ggaattgttc cgaatcaata ccgcttttgg tgctattttg
     2641 tgactttaaa actgctttaa tttcttcaaa atagtgtttc atatggtaaa tcccttccat
     2701 tttgttttgt tttcaatata ttatatacta aaaatggtac gggaatcaat gctgtttata
     2761 acacattcca aaatgaaata tacaaaccta attgatctga aatagtgcaa agaagtgagg
     2821 attttggagt tgcttctgct tttttatcag gcaacataaa acgtggtgat gcagacagga
     2881 ataatttgga gtataattaa atatagagag cataaattta gaatctcata atgccgtatt
     2941 gaattaaaac gtgaacttat gctgtgaatt tgtattccat agtctttatt atataatatg
     3001 attaataagc gacctaaaat tgttcttcac tacgaactta tgtaagataa ggggtgagat
     3061 ataccagtgg attggcgttc tcaaaacaca aatcagaaaa gagagcaaac taaaaaaata
     3121 attagtgaat accttgataa aattagctta ggggacagtg cggtcaggga agaatttatt
     3181 ttgaagttta agccctttat attgaagcag gtatttaagg caacagataa gtttgcggag
     3241 cctgaaaata gtgaagagta tagtgtcgct ctttttgcat tcaatgaggc tatagataca
     3301 tatgatgaaa aaagacaccc caacttttta gttttctgtg agcaggttat tcgaagaagg
     3361 ctaatagatt ataaaagaaa gaataagaaa aacagtatgg tctttccttt ttcttatttt
     3421 gaatctgagg ataacaggct ggaaaacaca attcttcaaa cggacacgga cgacggaatt
     3481 caaaggctgg agttcttgga ggacatggat cattttaagg atgagctggc ttgctttaat
     3541 atatctttta gagatcttgt tttatgtaca ccaaagcaca gggattcgag ggagtcatta
     3601 ataaacgtag caagaaccct tgcgggtaat gatgagcttt atgggaagct taaaaggaag
     3661 aaaaaaatac ctactatcga gttgctaaaa ttaaccagcg ttagtcgcag aaccatagaa
     3721 agaaacaaaa aatatataat tgcagttagc ttgattttga ggagtaatct cgatattttt
     3781 aaggaatatg ctgaatgttg caaggagagg agggccgata tgcgatgatt acaggaaaag
     3841 taattgatat ggacaaaaaa actgccgttg taatgacgga tgattttgcc tttattaatg
     3901 ttaaggtacg accggagatg gcggtgggct ataaatttga attcggtgaa ctcgatatag
     3961 ttaagtcaaa accccgatta aaagcctata ttcctattgc tgcttgcttt gccattttgt
     4021 tatctcttgc tataatgttt atgcatggaa gcataggccc aaaaggcata tatgcatttt
     4081 tggccgttga tataaaccca agtattgagt tttctataga ttataacaac cgggtggttg
     4141 gggtcaggaa tttgaacaag gatgcacgga cagttacaga caaccttgaa ctagtgggaa
     4201 agacagtgga tgaggcttta agggatgtga ttcaaaggtc taaagagtgt ggatttattg
     4261 tgccggaaag agaaaacata gtactggtgt ctgttgcccg caataaacaa gatcaaggga
     4321 aatcagacaa tgaaaagttg aagggtaata ttgacaggct tacagctcac gtaaatgatg
     4381 ttctttcggc tttagatgac agtaacattg aaacaaaggt tttaaaaatt acagatgagc
     4441 aaagggcaac atccttggaa aaggatatct ccatggggag gtatgttata tacctgaaag
     4501 caattgaacg aaacatggac cttactattg ataaaatgag ggatgttaat ctcagccaaa
     4561 ttttaaagta tattagcatt ggctatgggg aaggagatat cccagttgct tcaccttctg
     4621 cggcccatac accgtctggt gaacagtctc ctacaccgga tgaaacttca gctcctgttt
     4681 ctactgctac ttcggttcct gctgcaactg agggccttgt tgattcaact ccttcgcatg
     4741 gacaacctac accagtccgt acagctacgt atgatgcaaa agagactggt acaatgttgg
     4801 taaaaacacc tactcctaaa gctgttgcga cagtggcggt gaaaacacct actcctaaag
     4861 ctgctgcgac agtggcggtg aaaacacctg ctcctaaagc cactgctaca ccaccggtga
     4921 agacacctac tcctaaagcc actccccgaa agacgtcttc cggttcggta tgggttgata
     4981 attccaatgc caaaatcgat gagattcgta agagggatat aaagataacg gttttagata
     5041 caaatagaag agagttggaa ggagttacag tcgaagtaaa acagaccagg cacgcatttg
     5101 cctttggtac ggcacttaga agaagtggcc tggacaatcc ttcttatgtc gagttcgtta
     5161 agaaatactt taattgggct gtgtttgaaa atgaatccaa atggtatacc aacgaaccca
     5221 ccatgggaaa tgtgacatat gaggaagcgg attatatgta tgatttttgc cagagaaacg
     5281 gaattacggt tagaggccat tgtattttct gggaagatca aggaaaccag ccttcgtggt
     5341 tggggtggct tgacccgttt tcgttgcgtt ttgctgtgga taacaggttg aacagtgtgg
     5401 taagccattt caagggtaag tttgttcatt gggatgtgaa caatgaaatg cttcacggca
     5461 gcttctttaa agaccgcctt ggagaatcta tctggccgta tatgtttaac aggacaaggg
     5521 aaattgaccc caatgtaaag attttcgtta acaacaatgt aaccactgtt aaggagatcg
     5581 atgactattt ggctcagata aactggctta agtcacaggg agtgaaaatt gacggtatcg
     5641 gactgcatgg tcattttggt gaaaaagttg accgggatgt cgtaaagggc atgctggaca
     5701 aactgtcctc cgcaaatatt ccgatatgga ttaccgagta tgattcctgt acttccgacg
     5761 aaaacagacg ggcagataat ctcgaaaacc tctaccgtac tgctttcagc catccatcgg
     5821 tccagggaat ccttatgtgg ggcttctggg aaggagctca ttggaaagga agagatgcgg
     5881 caatagtaaa ctatgactgg acattgaatc aagccggtaa aaggtttgaa gcactaatgg
     5941 aggaatggac aacaaaagaa tcgggaagaa cggatgagac aggaagcttt tatttacgcg
     6001 gattctacgg tgagtatgaa ataaccttga ctgtaccggg aaaaggaaaa actgtgcgac
     6061 gcttaaccct tagcccggga aaaggtgcat tgcagcaaac ctttgttatt gattaaaaga
     6121 ttgacgcggg attatgaaat gtgaagataa atatgtacct gtttcaattc gtaatatttc
     6181 actttcctat ttataaatgc agccaattga gattgttaaa aaaagaggag acatcgatgt
     6241 ctcctctttt ttaacgattc taaagtctat tgaaaatctt taaaggaatc tattttttta
     6301 agtacatatc ttttaatcag tgtgagatcg gtggaatcta ctgatccatc tttgtttaca
     6361 tctgcattgg tacgtgctgt ttctgacaga tcaagttttt taagtaaaaa tctcttcatc
     6421 attgtaacat cggtggaatc cactgactta tcatcattta agtctccgta gataatgtct
     6481 gtagaagaca ttggaacgaa tgtaaaccag tcgaaattaa caggtccggt aaataccaga
     6541 tagaggtttt tgactcccgt aaccttgttt gttatgttac attgctgctc ctcataagaa
     6601 tcccaatctc ccgtggattg aactgccaga gtacctaaaa gggtaccgga cgggcttcct
     6661 gcccttatct gaatgtttgt agttgtctct gcggcagatg caaccattgc tttaaaggaa
     6721 agggctccat ccttgccaaa gtccacgtta ttatatacta catagtctcc gctttcaata
     6781 tagccgatac cttcgccgcc ttttccatta ccgattattt cgatggttga ggaattaaga
     6841 tcgctgaaat tctctgcctc caatttggaa taggcatctc tttgggtatt gtccggtgtt
     6901 ctggtaggag taggtgtcgg ttttgtggtc ggtgtagggc ttgatgaatt gcccaaatat
     6961 tgtttcaacc ataccattgc tggacgctca gtacccgatg aggtaatgag atgtgtatta
     7021 tccttccagg tctgtccctg tatccatccc cacagtgtta cacctttaac agagggatac
     7081 tcccataaaa cagggaattt ttcttgatac ctctgcaatt gcgtactatc gtcgccggtg
     7141 atatcaagtt cggatacgta tatcggaagt cctgttgcag ctaacttatc aaggttgctc
     7201 ttcattgtgt ttacggatac ggtatctata ttgaagcaat ggcattgaat acctataccg
     7261 tctaccaaac ctctttcctt gagcaggtta ataatctgta tgtattggtt tgttgcattg
     7321 ccgtcattta ttattccgta ttcattaata agaagttttc cgctaaatct ctttcttgct
     7381 tcttcgaagg accagatgac ccaatcccag cccgtggaac cgtctcctcc tattgcattt
     7441 ctatatgagg gttttgcatg caagggctca ttaacaacat ctacatactc tgaatctcca
     7501 aacttttctg cagcggcatc aaaccactcc aaaacttcag ctttctgctc ggaagcggaa
     7561 agtccgctga cccatcccgg ttcctggctt ccccatacca gtgtatggaa cttgaagggg
     7621 aacccgttgc ttttcgcata gttataagcc atattagcct gggaccagtt cattacatct
     7681 ctggaggatt caacagaacc ccatttggtt gaattctccg gtgttacctg attccaatac
     7741 tgagcgaagt ttgaaggaat attggatgct ataatttgtc ctaaaaactt ttcaccggta
     7801 tcgggatttt gtgtaggtct ttgtgttgat gcaggtgtag gggtggggtt tgaaccaccg
     7861 gaagtaaaag tgaacgagtc caaattaacg gctccgctaa ataccagata gagatttgca
     7921 acccctttga tactgttgtt taaactgcag gtatgttcct tatattcatt ccagccacct
     7981 gtggattgaa ctgcaagggt acctaaaagc gtaccgcttg ggcttccggc tcttaactgg
     8041 atgttagtag ttgtacttgc tccggatgcg gccactgctt taaaggaagt ggctccgttt
     8101 ccaaagtcca cattgttgta taccacataa tctccgttct cgatatatcc gataccgctg
     8161 ccgccgccac
//
LOCUS       DAOOJC010000047         8157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_7539_length_8157_cov_3.584300, whole genome shotgun sequence.
ACCESSION   DAOOJC010000047 DAOOJC010000000
VERSION     DAOOJC010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7539_length_8157_cov_3.584300"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2352)
                     /locus_tag="PLA01_02335"
     CDS             complement(<1..2352)
                     /locus_tag="PLA01_02335"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HPD00170.1"
                     /translation="MKRGLFILLVAFSLIFNSSGVAAQKLLLTYDGAVHEYTGNIFSL
                     KVNGEEIKSDIPPIIINGRSLVPVRAIFEKLGAQVFWDATEKKVTVSYNGKDIVLKIN
                     DTYATVNGEKVKMEVPAKIINDRTVVPLRFVGESLDMEVGWYPEKGEITIDNKTVVAQ
                     INDVKYSNANNSHQVKIDLDTCRNYKIMRVSEPDRIVVDFPNTKLSSVSPSITVEDEL
                     VKSIRCGQFDESTARVVMDVVGKPEYLVKEDEESIVLNLLPKSSDTETDGQKGEQDEP
                     GKAGGDSNGNFLNTRHVSKTDHEEVHIKVNKIDNFDSFMITEGTPRIVVDIPDSRIEG
                     ELQRIDVNSSLVSAIRCANQEGNAARVVVDLKGISNYKVMKSGDYIVVYVSNNRIAEN
                     PEISLPSRGDGGMVEKDDIILYVAYVPQESREEVVLSINSYENYNISKNMSNNSIILE
                     IPNAMGPLAEKTISVDSSLIKHIKYVGVDGASARVTVQLNGKSQYRVEEDKGKMTLII
                     SEMTDVGDFIPFTPTPTPTPTPTPTEKPKLTATPIITPTPNGTGVKTPNKTPKAGGSN
                     ELSIDYVINGGYNKVFLNIENYESYNAWRLSEPDRIVIDVTGAQADKDQKTIEIKSSL
                     IDSIRYARFEQDVARVVIDIAGPIQYHIEEFDGGLIVYLLESTLENVVYSNSMDRKHF
                     IIKGAKLTEGGENLKKLYKESYGFEGRRFTITFPSNLADIGSGIMRINDGIVDYVQVN
                     VNEKTGETSIEFYTFDSYNYVIITRSETKDTAITLLKRYSKDDK"
     gene            complement(2583..3686)
                     /gene="pilM"
                     /locus_tag="PLA01_02340"
     CDS             complement(2583..3686)
                     /gene="pilM"
                     /locus_tag="PLA01_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPD00171.1"
                     /translation="MLLPFVRNNYLSIDVGFRNIKVVEVAVNRNNEVYIKNFGIAPTP
                     INCIKNGVIKNVDAISSEISRIIRENKMKVKKSKIVMSGTSIISRVFMVEKVEGKDIN
                     DIVADAIRSNMPINPEEHKIDFKILQELKENGVEKMKIFVTAVRKSIINSYIDVLFEL
                     GLKPISVDIPANSTAKFFNRDIKVSIDNVRFIGQNKSQLNKDAFAVIDFGSETTIINI
                     LRNKILEFNKVILTGSTNLDEAISKTARKSIIEAERLKKLYGITVPPPHTTNEDHLKV
                     YRTLKDVVDKLLNQIYMCLTFYEARCYGVKVGKIFVIGGGSLLKGLSDYMEQVLQIPV
                     YPVGLLDIDGIDINKNLNSEKLNFLVNAVGITL"
     gene            complement(3832..4055)
                     /locus_tag="PLA01_02345"
                     /pseudo
     CDS             complement(3832..4055)
                     /locus_tag="PLA01_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821793.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 family protein"
     gene            complement(4234..5226)
                     /gene="ruvB"
                     /locus_tag="PLA01_02350"
     CDS             complement(4234..5226)
                     /gene="ruvB"
                     /locus_tag="PLA01_02350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPD00172.1"
                     /translation="MEDRLVDCKLKDEDVEELSLRPRRLSEYIGQTKVKENLVVFIEA
                     AKKRKEALDHVLLYGPPGLGKTTLAGIIASELGVNIRVTSGPAIEKPGDLAAILTNLG
                     NFDVLFIDEIHRLNRSVEEILYPAMEDYALDIIIGKGPSARSIRLDLPKFTLIGATTR
                     AGLLTSPLRDRFGVINKLDMYTTEELKLIVQRSAQILNAGIEDQAAEEIARRARGTPR
                     IANRILKRVRDFAQVKSDGFITKDIARIGLDALEVDKMGLDAVDRNLLMSIIDKFAGG
                     PVGLETLAATIGEEAGTIEDVYEPYLLQLGFINKTPRGRMATKLAYEHFGLRYE"
     gene            complement(5271..5879)
                     /gene="ruvA"
                     /locus_tag="PLA01_02355"
     CDS             complement(5271..5879)
                     /gene="ruvA"
                     /locus_tag="PLA01_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPD00173.1"
                     /translation="MFAYLKGRLAYKYNDYLVVEANGIGYRLFTALSTLESIGSVGDE
                     VKVYTHLYVREDNISLYGFGTEEELGIFELLIGVSGVGPKAAISVLSSMTPSRFSLAV
                     ITDDIKALTKAQGIGTKTAQRIILELKDKINKEQLTAKDGLETDNGNVGNEGSRVSEA
                     ISALMILGYTSLEARKAVMSVYTEDMDLEVIIKNALKRLVRS"
     gene            complement(5913..6401)
                     /gene="ruvC"
                     /locus_tag="PLA01_02360"
     CDS             complement(5913..6401)
                     /gene="ruvC"
                     /locus_tag="PLA01_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPD00174.1"
                     /translation="MIIMGIDPGFAITGYGIVKYEGNKFSPIDYGAVTTDSSMELPKR
                     LLLLYNGLTDIIKKFRPEAIAIEELFFNKNTKTALNVGHGRGVAVLAVAQSGIDVFEY
                     TPLQVKQAVVGYGRAEKAQIQQMIKVILNLSEIPKPDDVADALAVAVCHGSSYKMACI
                     GR"
     gene            6599..7816
                     /locus_tag="PLA01_02365"
     CDS             6599..7816
                     /locus_tag="PLA01_02365"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPD00175.1"
                     /translation="MSQKEKVILAYSGGLDTSIIIPWLKENYDYEVIAMAADVGQGEE
                     LEPLNEKAIKTGASKIYIEDLKEVFVTDYIFPTLKAGAVYEGKYLLGTSFARPIIAKR
                     MVEIAEKEGATAIAHGATGKGNDQVRFELTVKALAPHLKIIAPWRIWNIKSREDAIEF
                     AEARNIPIPVSKKDNYSMDRNLWHLSHEGSDLEDPWNEPQYDKILKLMVPPEKAPDKP
                     TYVDIYFDKGIPKKVNGKECSPVELIEVLNKIGGENGIGIVDMVENRLVGMKSRGVYE
                     TPGGTILYAAHRELELLCLDRDTLHYKDIVSQRFAELVYYGQWFTPLREAISAFVDVT
                     QQTVTGTVRLKLYKGNVMSAGAKSDYSLYSEELSTFGEDAVYNQKDAEGFINLFGLPL
                     KVNALMKEKNKGK"
     gene            7826..>8157
                     /locus_tag="PLA01_02370"
     CDS             7826..>8157
                     /locus_tag="PLA01_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HPD00176.1"
                     /translation="MKLWGGRFEKSTDKSVDDFNSSIRFDSRMYKQDILGSIAHAKML
                     GKCNIIPEEDSVLIQNTLKEILKDIEEGKIEFEIDAEDIHMNVEKILISRIGDVGKRL
                     HTGRSRND"
BASE COUNT         2372 a         1845 c         1406 g         2534 t
ORIGIN      
        1 tttatcatct ttagaatatc tctttaacag ggttatggct gtatcctttg tttcacttct
       61 tgtgattatt acgtagttgt agctgtcgaa ggtataaaac tctatacttg tctcgcccgt
      121 tttttcgttc acattaacct gaacataatc cactatccca tcatttattc gcattattcc
      181 gcttcctata tctgccagat tgctggggaa tgtaatagtg aatctccttc cttcaaaccc
      241 atagctttct ttatagagct tctttaggtt ttctccaccc tcggtcagct tggccccctt
      301 tataataaag tgttttctgt ccatactgtt actgtacact acgttctcta aggtagactc
      361 caacaaatac acaataagtc cgccatcaaa ttcctctata tggtactgga tcggccctgc
      421 tatatcgatt acaaccctgg ctacatcctg ttcgaaccgt gcatacctta tagaatcaat
      481 caaactgctt ttaatctcaa ttgttttctg atctttgtcc gcctgtgcac cagtaacatc
      541 aattacgata cgatcgggtt cggaaagtct ccatgcatta tagctctcat agttttctat
      601 gttaagaaag accttgttat agcccccgtt gattacataa tctatactta actcattgct
      661 tccccctgct tttggagtct tattcggtgt cttcacacca gttccattag gagtgggtgt
      721 tattattggt gttgccgtca acttcggttt ttccgtagga gtaggagtag gagtaggagt
      781 aggggtagga gtaaaaggta taaagtctcc aacatccgtc atctccgaaa taatcaaagt
      841 cattttccct ttatcttcct caaccctgta ttgggatttt ccgttgagct gaaccgtgac
      901 ccttgcagaa gccccatcaa cacccacata cttaatatgc ttaatcaagc tgctgtccac
      961 agaaatggtc ttttctgcca atggccccat ggcattgggg atctctaaaa taatgctgtt
     1021 attgctcatg ttctttgaaa tattgtaatt ttcgtagctg ttgatgctta aaacaacttc
     1081 ttcccggctc tcttgcggta catatgcaac ataaaggata atatcatcct tttcaaccat
     1141 gccgccatca cccctcgatg gcagggaaat ctcaggattt tcggctatgc ggttgttgga
     1201 aacataaacc acaatgtaat ctcccgattt catcacctta taatttgaaa ttcctttaag
     1261 gtcaacaacc acgcgtgctg cattaccctc ctggttggca catcttattg cacttaccaa
     1321 agaactgttt acatctattc tttgaagctc tccttctata cggctgtcgg gtatatcaac
     1381 cacgattctt ggtgttcctt ctgtaatcat aaaagagtca aaattatcaa tcttatttac
     1441 cttaatatgt acctcttcat ggtctgtttt tgatacatgc ctggtattta aaaaattccc
     1501 gttgctatcc ccgccagcct ttccaggttc atcctgctca cctttctgcc cgtccgtttc
     1561 ggtatctgag cttttaggaa gaagattcaa tacaatgctt tcttcgtctt cttttaccag
     1621 atactccggt tttccgacaa catccattac aacccttgct gtagactcat caaactgtcc
     1681 gcagcgtata gattttacca gttcatcctc cacagtaata ctgggtgata cactcgaaag
     1741 cttggtgttt ggaaaatcta caacaattct gtccggttcg gacactctca taatcttata
     1801 attccggcat gtgtcaagat ctatttttac ttggtgtgag ttgtttgcat tcgagtattt
     1861 aacatcattt atctgagcca caactgtttt gttgtcaatt gtgatttcac ccttttcagg
     1921 ataccaacca acctccatgt caaggctctc accaacaaaa cgcaaaggca caacggttct
     1981 gtcgtttatt atttttgccg ggacctccat ttttaccttt tctccgttta ctgttgcata
     2041 tgtatcatta atcttgagca caatatcctt tccgttatag gagacagtaa ctttcttttc
     2101 agttgcatcc caaaaaacct gtgcaccgag cttttcaaaa atagccctta caggaacgag
     2161 ggaccgcccg ttaattatta ttggtggaat atccgatttg atttcctcac cgttaacctt
     2221 gaggctaaaa atatttccgg tatattcgtg tacagcaccg tcgtaagtta atagcagctt
     2281 ttgggcagca acacccgatg aattgaaaat gagtgaaaac gccacaagca gaatgaacag
     2341 accccttttc ataaaaacac ctcttgtaaa ttttttcatt agtaaaaata taaaagccta
     2401 aatttcgact atatgcccta attaattatt cgacataatt atgaaaattc ctgtcataga
     2461 aatggttgta acggctattt gtaatagaac tgtaataaaa agcggatgtt tttgttgcac
     2521 aaaaataaaa aaagccttta atcaggcttt tttattatat ttttataaaa ttttatactt
     2581 cattaaagtg ttataccgac agcatttact aaaaagttca gtttttcact gttgagattc
     2641 ttgtttatat caataccgtc tatatccaaa agacccaccg ggtataccgg tatctgcaat
     2701 acctgttcca tgtagtcgct aagccccttt aacaaggatc ctccaccgat tacaaaaatc
     2761 ttgccgacct ttactccata acatcttgcc tcatagaagg taagacacat ataaatctgg
     2821 ttaagaagct tatcgacaac atccttgaga gtcctgtaca cctttaaatg atcctcattg
     2881 gtagtatgag gcggcggtac agtaatgcca tacaacttct taagtctttc cgcttcaata
     2941 atagacttcc gtgcagtctt tgagattgcc tcatcaagat tggtactgcc ggtaagtatt
     3001 accttgttaa actcaagtat cttatttctt aaaatattta taattgtggt ctcagagcca
     3061 aagtcaataa cagcaaaagc atctttgttc agttgactct tattttgacc tataaacctg
     3121 acgttatcaa tgctgacctt tatatccctg ttaaagaact tggcggtact gtttgcgggt
     3181 atatccacgg atataggctt taatccaagc tcaaataata catctatata gctgtttatt
     3241 atgctctttc taacagcagt aacgaatatt ttcatttttt caacgccgtt ttccttcaat
     3301 tcctgaagaa tcttaaagtc tatcttgtgt tcttcgggat taatgggcat attggatctg
     3361 attgcatcag caacaatatc gttaatgtct ttaccctcca ccttctcgac cataaataca
     3421 cgagagatga tactcgtacc agacataaca attttagatt ttttcacttt cattttgttt
     3481 tccctaatta tcctgcttat ttcactggaa atagcgtcaa catttttaat tacgccattc
     3541 tttatgcagt ttataggggt aggtgctata ccaaagtttt ttatgtaaac ttcattgttt
     3601 ctgtttactg caacctcaac aactttgata tttctaaacc ctacatcaat acttagataa
     3661 ttgtttctaa caaatggtaa caacatacat cattgactcc cttaacccca gaattgaacc
     3721 aaattttaat tataaaatct tttcaattat acattaacac aatctagaaa aaaaatcaat
     3781 atttatgctt aaaatatcca attaataaat tcataataag caaaaaataa attatttctt
     3841 caaacgcagc aaaaacatga tgattgtaag aatcaaacta ataatcaagc tagtagccag
     3901 tgggaaataa aacgtaaaat ttcctttctg taaactatgt ctccaggaat cctttccaat
     3961 cccagttttc cgcctaaata gagcaacaca cccaccagca gtaaaactgc cccgaaaata
     4021 attatcattc tgccaatagt ctgaaatcca tacatataat atcccaccac ctttattaca
     4081 cttcagtgca aagaaaattt cccgtttatt gaaattttct tacatcataa gtgtttcaac
     4141 gagacacaag atatcctttc tttaattttt cgcttcatta taggatatta tacatcctca
     4201 ttactatttc agtataatcc ttaaactata gctttactca tacctcagcc caaaatgctc
     4261 ataagcaagc tttgtcgcca tccttcccct gggcgtcttg ttaataaaac ctagctgcaa
     4321 aaggtacggc tcgtatacat cctcaatggt tccggcttct tctcctattg tggcggcaag
     4381 ggtttcaagc cctacaggac cgccggcaaa cttgtcgata atactcataa gaagatttct
     4441 atccaccgcg tcaagtccca ttttgtcaac ctccagtgca tcaagaccta tccgggcaat
     4501 atctttcgtt ataaatccat cggacttgac ctgtgcaaaa tctctgaccc ttttcaatat
     4561 cctgttggca atccttggcg ttcccctggc acgccgggca atttcttctg cagcctgatc
     4621 ctcaataccg gcatttagaa tctgtgcaga cctttgcact ataagcttta attcctcagt
     4681 cgtatacata tcaagcttgt tgattacccc aaacctgtcc cttaaagggg aagtcaaaag
     4741 tcctgccctg gtagtagccc ctatcagggt gaatttgggc agatccagcc ttattgaacg
     4801 ggcactgggt cccttcccga tgattatatc aagggcataa tcctccatgg caggatacaa
     4861 tatctcttcc acactcctgt tcagcctgtg gatttcatca ataaaaagga cgtcgaaatt
     4921 cccgaggttg gtcaatattg cagccagatc ccccggcttt tcgattgccg gaccggatgt
     4981 aactcgtata tttactccaa gctcggatgc aattattccg gcaagcgttg tctttcccaa
     5041 tccgggtggt ccgtaaagaa gcacatggtc caaagcctct tttctttttt ttgcagcttc
     5101 gataaatacc accaggttct ccttaacctt tgtctgtcct atgtactcac tcagtcttcg
     5161 gggcctcaga ctgagttcct ctacatcctc gtccttcagt ttacagtcaa caagcctgtc
     5221 ctccatatca aaaacctccg aatatgtaaa taaattgaat attcaattcc ctaacttctt
     5281 accagccttt taagggcatt tttgattatc acttcaagat ccatatcttc cgtataaaca
     5341 ctcataacgg ctttccgggc ttcaagcgat gtatacccca gaatcatcaa tgcacttatc
     5401 gcctccgata ccctggagcc ttcgttgcct acatttccgt tatccgtttc aagaccgtct
     5461 tttgcagtaa gctgttcttt atttatcttg tcctttagct caagtattat tctctgagcc
     5521 gttttagttc caatcccctg tgctttcgtc aatgccttta tatcatcggt tatgacggcc
     5581 agactgaacc ttgaaggggt catggacgac aataccgata tggccgcctt tggcccaact
     5641 cccgaaacac cgattaaaag ctcaaaaatc cccagttctt cttctgtgcc aaagccatag
     5701 aggctgatat tgtcctccct cacataaagg tgagtgtaaa cctttacctc gtcgcccacc
     5761 gaacctatac tctcaagggt ggaaagtgcc gtaaacaacc tgtatccaat cccgttcgcc
     5821 tcaacaacaa gataatcgtt atacttatat gccaacctgc cctttagata tgcaaacaat
     5881 tcttatatcc tccacattat attctgaaaa ttttatcgtc caatgcatgc cattttataa
     5941 gaactcccat ggcaaacagc aaccgccaat gcatcagcta catcatcagg ttttggaatt
     6001 tcagaaagat tcagaatcac ctttatcatc tgctgtatct gagccttctc tgccctgccg
     6061 tagccaacta cagcctgctt cacctgaagt ggggtatact caaaaacatc aataccggat
     6121 tgtgcaacgg ccaaaaccgc aacacctctg ccatgtccta cattaagtgc agtctttgtg
     6181 tttttgttga aaaacagttc ttctattgct attgcctcgg gcctgaactt ctttataata
     6241 tccgtcaaac cgttgtagag aagcaaaagc cttttaggaa gctccattga actgtcggtc
     6301 gtaaccgctc cataatcaat gggagaaaac ttgttccctt catattttac tataccgtat
     6361 cctgtaattg caaatccggg atctattccc atgataatca tttatttaat caactccaaa
     6421 aaaattatga ataattatat atttcgttgc atatttatac attttattat ataatatatc
     6481 tcattataat gaaagcatca actttgtaca tactattata aaccgcatca agcaaataaa
     6541 attgatattg ttgcttaaat attaaattat attctaacac atttgaggag gtatttgtat
     6601 gagtcaaaaa gaaaaagtaa ttcttgccta ttccggaggt ctggatacat caataattat
     6661 tccctggcta aaggaaaatt atgattacga ggttatcgca atggctgctg acgtaggtca
     6721 gggagaagag cttgaaccct taaacgaaaa ggcaattaag accggtgcaa gcaagatata
     6781 tatagaggac ttgaaagagg tatttgttac cgactatata ttccctacat taaaggccgg
     6841 tgccgtatac gaaggaaagt accttttggg aacatccttt gcaagaccga ttatagccaa
     6901 aagaatggtg gaaattgccg agaaggaagg tgctaccgcc atcgcccatg gtgcaacggg
     6961 aaagggaaac gaccaggttc gctttgagct taccgtaaaa gccttagctc cgcacctaaa
     7021 gatcattgcc ccatggagaa tatggaatat taaatcaagg gaagacgcca ttgaattcgc
     7081 agaggcaaga aacatcccca ttcccgtatc caaaaaagac aattacagca tggacagaaa
     7141 tctctggcat ttgagccatg agggttcaga ccttgaggat ccatggaatg agccgcaata
     7201 cgataaaata ttaaaactta tggttccacc tgaaaaagca ccggataaac cgacctatgt
     7261 tgatatatat tttgacaagg gtattcccaa gaaagtaaac ggcaaggaat gcagccctgt
     7321 tgaattaatt gaggtattga ataaaatcgg cggtgaaaac ggtatcggaa ttgtggacat
     7381 ggtagaaaac agactggtag gaatgaagtc cagaggagtt tatgagactc cgggtggaac
     7441 tattctttat gctgcccata gggaattgga gcttctctgt ctcgaccgtg acactctcca
     7501 ttacaaggat atcgtatctc aaaggtttgc cgaactggtt tattacggtc aatggtttac
     7561 gcctcttcgt gaagccatat ccgcttttgt cgatgtcacc cagcagacag tgacgggtac
     7621 tgtaaggctt aagctttaca agggtaatgt aatgagtgcg ggagcaaaat cggattactc
     7681 tctgtacagt gaagagctct caaccttcgg tgaagatgcg gtctacaatc aaaaggatgc
     7741 ggaaggattt atcaatctct tcggtcttcc gttaaaggtc aatgcactca tgaaagaaaa
     7801 gaataaaggc aaataaggtg atgaaatgaa gctttggggc ggtagatttg aaaagagtac
     7861 tgacaaatcc gtggatgatt tcaattcatc cataaggttt gacagccgca tgtataaaca
     7921 agacattcta ggcagcattg cccatgctaa aatgctcggt aaatgcaata taattcctga
     7981 agaggattcg gtattgattc agaataccct caaggaaata ctgaaagaca ttgaagaagg
     8041 caaaattgaa ttcgaaatcg atgctgaaga catccatatg aatgtagaaa aaatcctcat
     8101 atcaagaata ggtgatgtgg gtaaaaggct tcacacagga agaagccgca acgacca
//
LOCUS       DAOOJC010000048         7986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_7705_length_7986_cov_3.704955, whole genome shotgun sequence.
ACCESSION   DAOOJC010000048 DAOOJC010000000
VERSION     DAOOJC010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7986
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7705_length_7986_cov_3.704955"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLA01_02375"
     CDS             <1..348
                     /locus_tag="PLA01_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HPD00177.1"
                     /translation="VEWVRYPGLKDSPYHELAQRDFPKGAGGILSFGFKGTTEQREAF
                     LNSVKLFHYHVNIGDARSLIVNSPQTTHSELEPDEKKIADIPENLIRISAGLEDSADL
                     IADLEQAFKKANT"
     gene            373..1434
                     /locus_tag="PLA01_02380"
     CDS             373..1434
                     /locus_tag="PLA01_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPD00178.1"
                     /translation="MKGFRKLIGTVALFSVFISLLSGCGPKNTGGSQGSGKAGTSSTE
                     YKYGKIDIPGKDGSLCGAPIYIAYEKGFFKEQGFDVNLISADTETRKIGLNNGTIPIV
                     NGDFQFFPSIENDVKVKVVDGLHYGCIKLIVPKDSPIQGVQDLKGKKISVDEIGGTPH
                     QVASVWLEKNGISAKQEDGEVTFLPFSDGNLAVEALRKGDVDVAALWDPFGSVQEKTG
                     DYRVILEISKDEPFAGKYCCFLYASEKLLEEKPEQVAALLRAYRSAQNWISENPEEAV
                     DIIISGKYAQIEDRELAIKLIKSYQYPSFAEREKSKTQVRDNVFYFAEQLNQIGYLKT
                     DPDTFTDDAYTEVDINLGS"
     gene            1516..2583
                     /locus_tag="PLA01_02385"
     CDS             1516..2583
                     /locus_tag="PLA01_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD00179.1"
                     /translation="MYNSLKLKSLTSSELKVKQKYGKLKTVSAQNWGANFVLTLIGFA
                     VAISANILVKQVADVEIKTYIVAGIPFTFNLLYRLVLGVIVLFYAGIGIFSYFDPVKR
                     DKFSRRAPFRFAMGLVITVWDILGTKLLLLPQPFFPGPAKILESFLIEGGFILENALY
                     SIKLFLVGFTLGVVFGVGTGILIGWFPKVYYWVYPVLKITGVIPAVAWMPFALTLFPS
                     PFTAAVFLIVICAWFSIAALTAQGIQSTPKIQFEVARTLGAKTPFLVFHVAVPQAMPQ
                     IFTGISNANGFAFTTLVMAEMMGQPGGLGYYINLSKAWSAYYKVFASILVMAVLFSLI
                     MKILGLIQANVLRWQKGWVKE"
     gene            2580..3383
                     /locus_tag="PLA01_02390"
     CDS             2580..3383
                     /locus_tag="PLA01_02390"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD00180.1"
                     /translation="MSKNLSVISDVILHIENVNYTYYDDTGSHVEVLRDINLSVRRGE
                     FLSIIGSSGCGKTTLLRLIAGLDKPETGTLILNGEQIFGTDPQRGYVFQHGGLFQWLT
                     VEKNIATGLKARHVYKENKDRVAHYIDLIGLKGFEKSYPHQLSGGMAQRVAIARALIN
                     NPKVLLLDEPMSALDSFTRSDIQDKLLELHKENKTTMILVTHDVDEAIYLSDRIVIMT
                     PRPGKISEIMQINLPHPRHRGGTEFLSLRRNILEKLHLASALPQPEYTI"
     gene            3403..4257
                     /gene="nifH"
                     /locus_tag="PLA01_02395"
     CDS             3403..4257
                     /gene="nifH"
                     /locus_tag="PLA01_02395"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016611 - iron-iron nitrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_component: GO:0016613 - vanadium-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HPD00181.1"
                     /translation="MSKEIRQIAIYGKGGIGKSTTTQNLTAGLAESGKKIMVVGCDPK
                     ADSTRLLLGTLAQRTVLDTLRETGEDVKLENIMRTGFSGIKCVESGGPEPGVGCAGRG
                     IITSIGLLEQLGAYDDDLDYVFYDVLGDVVCGGFAMPIREGKAKEIYIVASGEMMALY
                     AANNIAKGIAKFAQKGGVRLGGIICNSRNVDREIDLLRVFSSELGTQLIYFVPRDNVV
                     QRAEINRKTVIEYEPNSQQADEYRKLAKAIDENTHFAIPKPMSQERLEEILLEYGLME
                     SLRDDYRI"
     gene            4511..5071
                     /locus_tag="PLA01_02400"
     CDS             4511..5071
                     /locus_tag="PLA01_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HPD00182.1"
                     /translation="MKRLIAYLIICVLMAVSFVACSKTKQSEPVKTIKPGEKIQITSS
                     SLTDDGKWKTVITSSDGENKSPQLSWTPVENASCYAIYMIDTSANNWCHWVAKDIEVT
                     ELELGAELENSRYIGPYPPSGVHTYEVMVFAIKDSPDSYKGLFDNANSSVDDIIAALD
                     VSKGTNGNILASGMLSGTYQTGDIVE"
     gene            complement(5047..5976)
                     /locus_tag="PLA01_02405"
     CDS             complement(5047..5976)
                     /locus_tag="PLA01_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPD00183.1"
                     /translation="MELFTRSYDKDWNRIVYLKVFENREACLHLNDDQTCKLILFLEG
                     NSTITYNNQTAFIIAPSVLCLNHGDTIEFDDISNCKIMVLFFQPIALNDQLKYSVFHP
                     DYYQNMAGTTLFQDMTLLSSFYDINNSKRQLILLDAVSAVSLKQLLSKINKELTDQKD
                     GYWPCRSRSHFIELLFFLEGLRTNASLQNMSIIVDKNKSNIIHRIVQYLNQNIDKKIT
                     LEDLEKTFGCNRNQINKEFQRELNTTVMKYFTQMRMQLASILLRDTEIPILEVAFKVG
                     YSDAAYFSRAFKLYCGTSPSEYRSSFYSTISPV"
     gene            complement(6120..6932)
                     /gene="larE"
                     /locus_tag="PLA01_02410"
     CDS             complement(6120..6932)
                     /gene="larE"
                     /locus_tag="PLA01_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HPD00184.1"
                     /translation="MDTIYKKYNLLKDYIASLGSVAVAFSGGVDSTFLLKAACDALGD
                     SVIAVTASSCTFPVRELKEATKFCEDNNIKHIICQSEELDIDGFRQNPKNRCYLCKRE
                     LFTKIWDIAKENRMNAVIEGSNLDDDGDYRPGLIAVKELGVKSPLREMQFTKDEIRQL
                     SKELGLPTWSKQSFACLSSRFVYGETITEEKLSMVDQAEQLLLDLGFRQVRVRIHGMM
                     ARIELLPSEFEKLVQEDVRQLVYSRLKALGFTYVTMDLGGYKTGSMNATLKL"
     assembly_gap    7506..7515
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7516..>7986)
                     /locus_tag="PLA01_02415"
     CDS             complement(<7516..>7986)
                     /locus_tag="PLA01_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD00185.1"
                     /translation="LTVLFAVRSPAKRAARVSPLTAVSGNINYTQSIRTSAKTSIFKI
                     DTAVGIHHAMSGKKNFLLMVGSFSLSVVLFLCFSVTLDFMHHAIKPLRPWTPDISFIS
                     PDQSCSLPGTIIEQIKEYPKVKRVYGRMFAYSIPVVFWSQNKKANLLSYEDYQFS"
BASE COUNT         2381 a         1498 c         1762 g         2335 t
ORIGIN      
        1 gtggaatggg ttcggtatcc cggtttaaag gatagcccgt atcatgaact ggcacaaaga
       61 gactttccta aaggagccgg aggaattctg tcattcggtt tcaaaggaac aacggagcaa
      121 agggaggctt tcttgaactc agtgaagctt tttcattatc atgtcaatat tggagatgcc
      181 cgcagcctga ttgtaaattc tccgcagacc actcatagtg agctggagcc ggatgagaag
      241 aaaatagccg atattcccga aaacttaatt cgcatatcgg cagggctgga agattcggcg
      301 gatttaatag cggatttgga gcaggcgttt aaaaaggcta atacataatt ttgtaaaggg
      361 gtgtgtttta tcatgaaagg ttttagaaaa ttgatcggta cagtcgctct gttttccgtt
      421 ttcataagcc tactttcagg atgtggtccg aagaatacag gagggagcca gggcagcggg
      481 aaagcgggaa catcttcaac ggaatacaaa tacggaaaaa tcgatattcc cggtaaagac
      541 ggttcacttt gcggtgcacc tatctatatt gcatatgaaa agggcttttt caaggagcag
      601 ggctttgatg tcaatcttat ctctgcagat accgaaaccc gtaaaatagg tttgaacaac
      661 ggtaccatcc cgattgtcaa cggagatttt cagttctttc cctctattga aaacgatgtg
      721 aaggtaaagg tggtggacgg cctccactat ggatgcataa aattgattgt tccgaaggat
      781 tcgccgattc aaggggttca agaccttaag ggtaaaaaga tcagcgttga tgaaatagga
      841 ggtactcctc atcaggtagc atccgtgtgg ctggagaaaa acggaatttc tgcaaagcag
      901 gaggatgggg aagttacatt ccttcctttc tccgatggta acctggcagt ggaagccctg
      961 agaaaagggg atgttgacgt ggcggcactg tgggatccct tcggctcggt tcaggaaaaa
     1021 acgggggatt accgtgtaat tcttgaaatt tccaaggatg aaccttttgc cggaaagtac
     1081 tgctgtttcc tttatgcctc ggaaaagctg ctggaggaaa aacctgaaca ggttgccgca
     1141 ttgctgcgtg catataggtc agctcaaaac tggatttcgg aaaacccgga agaagctgtc
     1201 gacattataa taagcggtaa atatgcacag attgaagaca gagaattagc cattaagcta
     1261 ataaagagct atcaatatcc ttctttcgct gagagggaaa agagtaaaac acaagttcgc
     1321 gacaatgttt tctattttgc cgagcaatta aaccaaatag gatatttaaa gaccgatcct
     1381 gatactttta cagatgatgc ttataccgag gttgacataa acctgggttc ataacaatcg
     1441 ctagaggttg tttccgcccg gtgattaaaa ggatccgggc ggaaataata aacaacccac
     1501 aggaggtatt ctgctatgta caattcatta aaactaaaaa gcctaacatc ctcggaatta
     1561 aaagtgaagc aaaagtatgg gaagctaaaa acggtttcgg cccaaaattg gggtgctaat
     1621 tttgtactga cattaatagg gtttgctgta gccatatcag ccaatattct ggtaaagcaa
     1681 gtggcagatg tggaaatcaa aacttatatt gttgctggaa ttcccttcac atttaattta
     1741 ctttaccgat tggtcttagg agtaattgtt ttgttttatg ccggtatagg aatattttca
     1801 tattttgatc ctgtgaaaag agataaattt tcccgacggg ctccctttcg tttcgcaatg
     1861 ggtctggtaa ttacagtatg ggatatatta ggaacgaaac ttcttttatt acctcagccg
     1921 tttttccctg gacctgcaaa aattcttgaa tcctttttga ttgaaggcgg ttttatttta
     1981 gaaaatgccc tttactccat taaacttttt ctggtcggat ttactctggg ggtagtgttt
     2041 ggggtaggta cgggaatttt aataggttgg tttccaaagg tatattactg ggtatatccg
     2101 gtattaaaga ttaccggcgt tattcctgcc gtagcttgga tgccctttgc actgactctt
     2161 ttcccgtctc cgtttacagc agctgttttt cttattgtaa tatgtgcatg gttttccatt
     2221 gcggctctaa cagcccaggg catacaatcg acccccaaaa ttcagtttga agtggctcgc
     2281 accctcggag caaaaacacc gtttttagta ttccatgttg cagtgcctca ggctatgccc
     2341 cagatattca ccggtatttc caatgccaat ggttttgctt ttacaacttt ggtgatggca
     2401 gagatgatgg gccagccggg aggattaggc tactacataa acttaagtaa agcgtggtct
     2461 gcctattata aggtatttgc ctcaattctt gttatggccg tactgttcag tctaattatg
     2521 aaaatactgg ggttgataca ggcgaatgtg ctccggtggc agaaaggatg ggttaaggaa
     2581 tgagcaaaaa cttatcggta atcagtgatg ttattttaca tatagaaaat gtaaattaca
     2641 cttattatga cgacaccgga agccatgttg aggtgcttcg ggatatcaat ttatccgtac
     2701 gccggggaga atttctttca atcataggct cctccggctg cgggaagacg acgcttttgc
     2761 ggcttatagc aggtcttgac aagccggaaa caggaaccct gattcttaac ggagagcaaa
     2821 tcttcgggac ggatccgcag aggggctatg tttttcaaca tggggggctt tttcaatggc
     2881 tgactgttga gaaaaatatc gcaaccggac ttaaggcacg ccatgtatat aaggagaata
     2941 aagaccgtgt ggcccattat atagatctga tcggacttaa gggttttgaa aaatcttacc
     3001 cgcatcagtt aagcggaggt atggctcaga gagttgctat tgctagggct cttataaata
     3061 atccgaaggt attgcttctt gatgaaccca tgagtgccct tgattccttt acccgttcag
     3121 atattcagga taaattgctt gaacttcata aagaaaataa aacgacaatg attttggtga
     3181 cccatgatgt ggacgaagca atctatcttt ccgaccgtat tgtgattatg actccgcgtc
     3241 ccggaaaaat cagtgagatt atgcagatta atcttccgca tccccgccat cgtggaggga
     3301 ccgaatttct ttccctccgc aggaatattt tagaaaaatt acatctggca agtgcattgc
     3361 cgcagccgga atatactata tagattatgg aggagagata atatgagcaa ggaaataaga
     3421 caaatagcta tttatggaaa gggcggtatt ggaaaatcta ctacgacgca aaaccttacc
     3481 gcaggacttg cggaaagcgg caaaaaaatt atggtggtag gatgtgatcc gaaagccgat
     3541 tctacaagac ttttattggg tactcttgca cagcgtactg ttctggatac actgcgtgag
     3601 actggggaag atgtgaaact ggaaaatatt atgcgtaccg gatttagcgg aataaagtgt
     3661 gttgagtccg gagggccaga gcccggcgtt ggatgtgccg gacgtgggat tattacctcc
     3721 ataggtttgc tggagcagct tggtgcctat gatgatgatc tggactatgt attttatgac
     3781 gtactggggg atgttgtttg cggcggattc gctatgccga ttcgtgaagg aaaggctaaa
     3841 gaaatttata ttgtggcaag cggtgagatg atggcattgt atgctgccaa caatatcgca
     3901 aagggaattg caaagtttgc tcaaaaggga ggagtgagac ttggaggcat aatatgcaac
     3961 agccgtaatg tcgaccgtga gatagatctt ctccgtgttt tttcttccga attgggaact
     4021 caactgatat attttgttcc tagagacaat gtggtacagc gggctgaaat caacagaaaa
     4081 actgttatcg aatatgagcc gaattcgcag caggccgacg aatatcgcaa gctggcaaaa
     4141 gcgatagatg agaatactca ttttgcaatt ccaaaaccca tgtctcagga acgtctggag
     4201 gaaattttgc tggaatatgg gttaatggaa tccctgaggg atgattatag aatttgattg
     4261 gagcggtcgg cagtttctgt tgaccgcttt acttagctaa atttcacgaa ctgaaatgac
     4321 caaaagtgat tggagctcga taccctcaga gcttttgttg atttttaagg attagtctga
     4381 gacaattcgg ctgcagaaaa ttctaaaaac atgtgcaaat tatccaagca tctgtgcaga
     4441 ttttttcttc aatgatatct ccacttccat tataattatc atagtagatt aaaagttgag
     4501 aggggatagt atgaaaagac taattgcata tcttattata tgtgtgctta tggctgtatc
     4561 ctttgttgct tgttctaaaa caaaacagtc tgagccagta aagacaatca aaccgggaga
     4621 gaaaattcaa attacttctt cttccttaac cgatgatgga aaatggaaaa ccgttatcac
     4681 atcatccgat ggtgaaaaca aatcaccgca attgtcctgg accccggtag aaaatgcctc
     4741 ttgttatgct atctatatga ttgatacctc tgcaaataat tggtgtcact gggtcgctaa
     4801 ggacattgag gtcaccgagc ttgaattggg tgcggagctt gaaaacagca gatacattgg
     4861 gccttatcca cccagcggag ttcatacata tgaggttatg gtctttgcca taaaggactc
     4921 tccggattct tacaagggac tgtttgacaa tgcaaattca tcggttgatg atattattgc
     4981 agcactggat gtctccaagg gaacaaacgg aaacatactt gcatccggaa tgttaagcgg
     5041 aacctatcaa accggtgata tagttgaata gaaagaacta cggtattcgc taggggaggt
     5101 accgcagtat aatttaaatg ctctggaaaa ataagcggca tcggaatacc ctactttaaa
     5161 tgcaacctca agtatcggta tttcggtgtc tcttaacaag atgctagcca actgcattct
     5221 catttgagta aaatacttca ttactgttgt atttagttcc ctttgaaatt ctttatttat
     5281 ttgatttcga ttacacccaa aagttttttc caaatcttcc agcgtaattt tcttatcgat
     5341 attttgatta agatactgga cgattctgtg tataatattg cttttattct tgtccactat
     5401 aatactcata ttctgcaatg acgcattggt acgtaatccc tctaagaaaa acagcaattc
     5461 aataaaatgg gatctgcttc tgcaaggcca atagccatcc ttttgatctg tcagttcttt
     5521 gtttatcttg cttagcaatt gttttaagct aacggcagaa acggcatcga gtaagataag
     5581 ctgacgtttg gaattgttaa tatcgtaaaa tgagcttaat aaggtcatat cctggaacag
     5641 cgttgtacca gccatatttt gataataatc tggatggaac acgctgtact ttaattgatc
     5701 atttaaagca attggttgaa aaaagaggac cattattttg cagttagaga tatcgtcaaa
     5761 ttctatggta tccccatgat tcaagcaaag gacagagggt gcgatgataa atgctgtttg
     5821 gttattatat gttatggtag aattgccttc aagaaaaagg atcagcttac aggtctgatc
     5881 atcatttaaa tgaagacatg cttctcgatt ttcaaatact tttaagtaca caatacggtt
     5941 ccagtcttta tcatagcttc ttgtaaatag ctccataaag tctctgcccc tctttcttaa
     6001 ttgttaacac cttggtttaa agaaaacttt tccgttgaag aaaaatttat ttaccttata
     6061 aataattatc cgaattaggt aaatccaatg gaatttaagt tacctaaatc ttatttgttt
     6121 cataacttca atgttgcatt catactgccg gttttataac ccccaagatc catcgttaca
     6181 taagtaaatc cgagagcttt aagccttgaa taaaccaatt gccggacatc ttcttgaacc
     6241 aatttttcaa attcagaagg aagtagctca attcttgcca tcatgccatg tatgcgaacc
     6301 cgaacctggc gaaagcctag atccaataaa agctgttcag cctggtccac catgcttaat
     6361 ttttcttctg ttatcgtttc gccatataca aatcgagaag aaaggcaggc aaaggattgt
     6421 ttactccatg ttggaagccc caattccttt gacaattggc gtatttcatc tttggtaaat
     6481 tgcatttccc gtagaggact tttgacaccg agctccttga ccgcaatcag cccgggtcga
     6541 tagtcaccgt catcatccag attagaaccc tcaatcacgg cattcatccg gttttccttt
     6601 gcaatgtccc aaattttagt aaaaagctct cgcttacata gataacagcg attttttggg
     6661 ttttggcgaa agccatcaat atccaattct tcagattggc aaatgatgtg tttgatatta
     6721 ttgtcctcgc agaactttgt tgcctcttta agttcccgca ccgggaaagt gcaggaggat
     6781 gctgtcacgg caattacgct atctccaagg gcatcgcatg cagcttttag aagaaaggtg
     6841 gaatcaaccc ctccggaaaa agcgacagca acactgccca gcgatgcaat ataatctttt
     6901 agcaagttat attttttata aatcgtatcc atggtaagta acccctttat actgacagta
     6961 ttggtatcca atagtgtcat ctcaaatttt atatctgcac tgaagcggtg tcacaacgag
     7021 gcgagggaca tacttgccga ccgaaaggct aatcggtacc cgccacttat agagatgcgg
     7081 ggatatttac cgcctcatta taatatatga atcacataaa tataatatat gattgaaaat
     7141 acagcatgtc aaggcaatat cagtttttga gattattata ttgttttatt aaagaataat
     7201 atgtattaaa gaataatatg aaataaacat tgtgaagtaa gttgtagagt ctgttaatac
     7261 gacgtggaaa ggaggagcat ccactctcac gaagtgagtt ttgctcgacc ccacgaagta
     7321 tttacaggct ctaagcttac gaaacttaag tttctgaaat gttttcacac aaaatatgca
     7381 ctctcaaact taagagtata ttaaataaaa ttaatataaa tatataacac tacagtgcac
     7441 tgatggtatc cacaatggac atgtcacgaa ttcgttttga aggcccgcga accgcaaaaa
     7501 cagacnnnnn nnnnngaaaa ttgataatct tcataagaaa gaagatttgc ttttttattt
     7561 tggctccaaa atactaccgg aatgctatag gcaaacatcc ttccgtaaac ccttttgact
     7621 ttcgggtatt ctttaatttg ttcaataatg gtgccgggca gtgaacaaga ctgatcgggg
     7681 cttataaaag aaatatccgg tgtccatggc cttaacggtt ttatagcatg gtgcatgaaa
     7741 tcgagtgtta cagaaaagca aaggaataaa acaacactta aagagaagga gccaaccatc
     7801 agtaaaaaat tctttttgcc ggacatggca tgatgaatgc caacagcggt atctatttta
     7861 aaaattgagg tctttgccga tgtgcggatt gattgggtat aatttatatt accggaaact
     7921 gcggtcaagg gtgaaacacg ggctgctctt ttagcaggag atctgactgc aaaaagaacc
     7981 gtaagt
//
LOCUS       DAOOJC010000049         7898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_7799_length_7898_cov_3.999872, whole genome shotgun sequence.
ACCESSION   DAOOJC010000049 DAOOJC010000000
VERSION     DAOOJC010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7898
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7799_length_7898_cov_3.999872"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PLA01_02420"
     CDS             complement(<1..851)
                     /locus_tag="PLA01_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPD00186.1"
                     /translation="MFSDFVNTIKGLISDSGFVKGDPLQYVMILIACFLIYLAIAKKY
                     EPLLLLPIAFGMLLTNLPFSNLYHPELFINESGHINLQELLNTGGLLDFLYLGVKLGI
                     YPPLIFLGIGAMTDFGPLLANPRSLLLGAAAQIGIFITLIGALLTGFFTGREAASIGI
                     IGGADGPTAIYVTTQLASHLLGPIAIAAYSYMALVPLIQPPIMKLLTTKKEREVVMEQ
                     LRPVSKLEKILFPIVVTIIVSLFLPSAAPLIGMLMLGNLFKESGVVDRISKTAQNELI
                     NIITIFLG"
     gene            complement(969..1346)
                     /locus_tag="PLA01_02425"
     CDS             complement(969..1346)
                     /locus_tag="PLA01_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HPD00187.1"
                     /translation="MSANLMQGLSITFVGVTVVFASLIILCAIIYLFSVLIPNKDKNS
                     SNKDSDEKLIDISSNNNAEAEKLESNLSDDTLVAVLTAAVLASMGDRPDIKIRVTSFK
                     RIQQTSPVWNVIGRKEYISNKLS"
     gene            1691..1879
                     /locus_tag="PLA01_02430"
     CDS             1691..1879
                     /locus_tag="PLA01_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00188.1"
                     /translation="MRQEDRIFINEIKNMIEFLIKENEKLTDTVNELKEEQKKLQEEI
                     KIQNIVLNSLPIRVDILN"
     gene            1943..2629
                     /locus_tag="PLA01_02435"
     CDS             1943..2629
                     /locus_tag="PLA01_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPD00189.1"
                     /translation="MNSKTRVLVIDDDVNICELIRLYMEKEGFEVLTVYNGTKALDGF
                     KNFTPNIVILDIMLPGADGWQVCREIRKISNIPIIMLSAKGETFDKVLGLELGADDYI
                     VKPFEPKELVARVKAVLRRYEHKDMDVQEIVYPNLVINKSNYTMKVNGKEMEFPPKEL
                     ELMFFLASNPNKVFTREQLLEQVWGFDFYGDSRTVDVHVKRLREKINSEGQSWQLKTV
                     WGVGYKFEVK"
     gene            2629..4149
                     /locus_tag="PLA01_02440"
     CDS             2629..4149
                     /locus_tag="PLA01_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD00190.1"
                     /translation="MLKTIFSRIVVIFIAILFVSTTITGAMLYFFLGNFASEEKVNLL
                     NRTGESITDMLSDYVSFYYSYYDSSYFPFLHSAYWSNMESILNAHSKNASSLIWIVST
                     DGQIRIMEGNQGIVNQIIEKKLRDEDGNLRLQNPSQYMDVMSGNKSMVKEMGDFYGLF
                     KETHVSWLTIEKPFIYDGEVLGAVYLHTPVPEVQRARSNVFKFFMVAVAVSIVISIIL
                     VYIFSLRLSRPLKKINSAAKHIANGEFNERLDIDSEDEIGELARSFNNMAGALQNLEN
                     MRRGFIANVSHELRTPMTSIHGFIEGILDGTVPPEKQKEYLTIVRDEVKRLNRLTTDI
                     LDLARIEAGEVSIKPIDFNINELIRRCIIKLESFITEKDINIEANFEEEEMYAKADID
                     SIERVVINLLHNAIKFVQPGGKITLSTSRSKNKILVCVEDNGIGIDSNEIDLIWERFY
                     KSDKSRSKEKTGTGLGLAIVRNIINDHRQKIWVESQVGKGTRFYFTLDSGTNGENI"
     gene            4252..5709
                     /locus_tag="PLA01_02445"
     CDS             4252..5709
                     /locus_tag="PLA01_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPD00191.1"
                     /translation="MEDNNYNGLNNYDEIINEQKDNGTVELNNDTGTNNTDNSYSSDV
                     SSGMENTQNYENAYGTKKESETVNKDLSSSPYYTESCKKPKKEKKGVLLQMIVVALMS
                     SIIGGSVVGSFFMFGIPALKSPVQSILGNGAVTGNDPDVSKNESDIYRKVVIESADSP
                     VVAIAEKVGPSVVGISVKSTSVNDFWFFSPSESEEQGSGIIIRSDGYIMTNNHVIEKA
                     LSGTTNNLVQNAKIQVILPSNPDKPYDATVVGRDTKTDLAVLKIDASSLPAVEFGNSD
                     DVKVGELAVAIGNPGGLEYMGSVTVGVISGLNRTIPITDDKELKLIQTDASINPGNSG
                     GALVNAEGKLIGVNTAKIGGIDFEGLGFAIPVNKAKEITDSLIEFKYVKGRPSLGIQV
                     NTGFTKEVADRFGIPEGILVYKVEIFSAAYKADIQEDDVITEFNGVRVKNYDELEEQK
                     SKFKPGDTVKIKIYRYHQEGKGEELTVDVVMDEQK"
     gene            6025..7422
                     /locus_tag="PLA01_02450"
     CDS             6025..7422
                     /locus_tag="PLA01_02450"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPD00192.1"
                     /translation="MPEKSVIPIIKLISIFLILILLIVQFISAPKLITGLVTVLVLLY
                     FITDYLTRTFSEHNNTNALSDLNKKAIKLEEELESLHEVSKIITSTFEVKTIIEYIYN
                     VLKRVTGCEWCHAYFIDKKSKIPVINYVYGNKNFADIGNLDYTKYVHTLIDINFSDDT
                     KIEILSDKSLGKIVAISLNVSNDTTGAVFIANPTEGTFSAPNFNFLKSIACYAAIGIR
                     NTEMFNNIYSQKEEIEALYEQSAANNEELNSYIKELDETKEELNRKNEELTRLFDEMQ
                     LGYLQTVMSLANSIEAKDPYTRGHCQRVMEISCELAKALKLTDKEIEDLRYASILHDI
                     GKIGISASILNKEGKLTDEEFDEIKKHPVIAYNILKDVEFIKSGLNGILQHHERYDGR
                     GYPHGIKGKEICIFARIMCVADAFDAMTSDRPYRKGMSMENALKEIERCKGTQFDPEI
                     ADLLIAMASSGIYIG"
     gene            complement(7555..>7898)
                     /locus_tag="PLA01_02455"
     CDS             complement(7555..>7898)
                     /locus_tag="PLA01_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00193.1"
                     /translation="HGLRAPSLFAISLREIALFSKAHGLRAPSLFAISLREIALFSKA
                     HGLRAPSLFAISLREIALFSKAHGLRAPSLFAISLREIALFSKAHGLRAPSLFAISLR
                     EIALHIQFKLN"
BASE COUNT         2677 a         1233 c         1737 g         2251 t
ORIGIN      
        1 cccaggaata ttgttattat gtttattagt tcgttttgtg cggttttact tattctgtcg
       61 acaactccgc tttctttaaa gaggtttccg agcatcagca ttccaataag aggtgctgca
      121 gagggtaaaa acaagctaac gataatggta acaactatcg gaaataaaat cttttcgagc
      181 tttgatactg gtcttaactg ttccatgacc acctcgcgtt cctttttggt tgtaagtaat
      241 ttcataatag gaggctggat taatggaacc agtgccatgt aggagtatgc tgcgatagca
      301 atcggtccaa gtagatgaga agccaactgg gttgttacgt atattgcagt ggggccgtcg
      361 gcacctccga ttattcctat ggatgctgct tctcgacctg taaagaagcc tgtcaaaagt
      421 gcacctataa gcgtaataaa aataccgatt tgggcagcag cacctaatag caggcttctg
      481 ggatttgcca gaagaggtcc gaagtccgtc atggcaccta tacccagaaa aatcagaggc
      541 ggataaatcc ccagctttac gccgagatat aaaaagtcaa gcaatccacc agtattcaat
      601 agttcttgaa ggtttatgtg accgctttcg ttgataaaca actcaggatg atacaaattt
      661 gaaaaaggta agtttgtaag caacataccg aaagctatag gcaacaaaag aagcggttca
      721 tatttttttg cgattgccaa atatatcaaa aagcaggcaa tcagaatcat cacatactgc
      781 aaaggatctc cctttacaaa tcctgagtct gaaataagtc ctttgattgt gtttacaaaa
      841 tctgaaaaca taaggctaat cttcctttac tttaaattct attttaattt gttattgaaa
      901 ctatttggtg tatatccaac atacggctta ttttatgtca aaaacaagag aaagcagata
      961 aaactatttt atgaaagctt gttggaaata tactcctttc ttccgatgac attccagacc
     1021 ggtgaagtct gttgaattcg tttaaatgaa gttactctta ttttgatatc cggtctgtct
     1081 cccatgctag cgagtactgc agccgtcaaa acagctacta gagtgtcatc ggataagttg
     1141 ctttctaatt tttctgcttc cgcattatta ttagaagaaa tatcgataag tttttcgtca
     1201 gaatccttat ttgacgaatt tttatctttg ttgggaataa gcaccgaaaa cagataaatt
     1261 atagcacaga gtattatcaa tgaggcaaaa acaactgtga ctccgacaaa ggtaatactt
     1321 aacccctgca ttagatttgc actcatatat acacctcccc catattatcg ttagaaaaat
     1381 tactcacctt gttaaagtta tcacaacagc ataagttagt caatagatac aattacatag
     1441 tgatatataa atcatatgta aagaaaaatg tggaagaaac ttaagttaca caagtttctt
     1501 acgcattttt cttttcttaa ataatataca cccgatacat aggattttta tagaagaatt
     1561 aattatagta ttcacaaagc aattattctg ctataatata caataacgaa gacaaaacaa
     1621 ctcaagattg gttatatttc cataatatat tctacttaat tattaattgg tttatcgggg
     1681 ggaataggat atgaggcaag aggacaggat ttttattaat gaaattaaga atatgattga
     1741 gtttttgata aaggaaaatg aaaagcttac ggatactgtt aatgaattga aggaggagca
     1801 aaagaagctg caggaggaga ttaaaattca gaacatagtt ctgaacagtc tgcccataag
     1861 ggttgatata ctgaactgat tattcaacaa ctgcaatgca ttggttccat taacagcgta
     1921 aaagttgagg tgattgggtg ttatgaatag caaaaccagg gtgcttgtca ttgatgatga
     1981 tgtcaatatt tgcgaattga taaggttata tatggaaaag gaaggcttcg aagttttaac
     2041 tgtgtacaat ggaacgaagg cacttgacgg atttaagaac tttaccccaa acatagttat
     2101 tcttgatatt atgttaccgg gagcggatgg atggcaggtt tgccgtgaaa taaggaaaat
     2161 cagcaatatt ccgattatta tgctttcggc aaagggtgag acctttgaca aggtactggg
     2221 cctggaattg ggagccgacg attatatagt aaagcccttt gaaccgaagg aactggttgc
     2281 cagagtgaag gctgtcctaa gaagatatga gcacaaggat atggatgttc aggaaatagt
     2341 ataccctaat cttgtaataa acaagtcaaa ctatactatg aaagtgaacg gcaaggagat
     2401 ggagtttccg ccaaaagagc tggaacttat gttttttctt gcatcaaatc ccaacaaggt
     2461 gtttaccagg gaacagcttt tggaacaggt ctggggattt gatttttacg gagattcaag
     2521 gactgttgat gttcacgtaa aaaggcttcg tgagaagatt aacagtgagg gtcagagttg
     2581 gcagcttaaa actgtctggg gcgttggata caagtttgag gtgaaataat gctaaagaca
     2641 atatttagca ggattgttgt tatatttatt gccattctgt ttgtgagtac caccattaca
     2701 ggagcaatgc tttatttttt tctcggtaat ttcgcttcgg aggaaaaggt gaatctttta
     2761 aaccgcactg gggaaagcat aacagatatg ctgtctgatt atgtttcttt ttattacagt
     2821 tactacgaca gctcttattt cccttttttg cacagtgcat attggagcaa catggaaagc
     2881 attttgaatg cccacagcaa aaacgcaagt tctcttatat ggattgtttc aactgacggt
     2941 caaatccgta tcatggaagg taatcagggg atagtaaatc aaataataga aaaaaagctt
     3001 agggacgagg atggaaattt aagattacag aacccaagcc agtatatgga tgtgatgagc
     3061 ggaaacaagt ctatggtcaa ggaaatgggc gatttttatg gtttgtttaa agaaactcat
     3121 gtctcatggc tgaccattga aaaaccgttt atatatgatg gagaggtttt gggagcagtt
     3181 tatcttcata cgcctgttcc ggaggttcag agggccagaa gtaatgtgtt caagttcttt
     3241 atggttgcag tggcagtctc aatagtaata tcaattatat tggtctatat tttttcatta
     3301 agactttcaa ggcctttaaa gaagatcaac agtgccgcaa aacatattgc gaacggagaa
     3361 tttaatgaga ggctcgatat cgattccgag gatgagatag gggaactggc aaggagtttt
     3421 aacaatatgg ccggtgccct tcagaacctt gaaaacatgc gcaggggttt tattgccaat
     3481 gtgtcccatg agcttaggac gccgatgact tcaatacatg ggtttattga ggggatcctg
     3541 gatggaacgg tacctcccga aaaacaaaaa gaatacctta ctatcgtcag ggatgaggtc
     3601 aaaaggctaa acaggcttac caccgatatt ttggaccttg ccagaataga ggcgggagag
     3661 gttagcataa aaccgattga ctttaacatt aatgaactta taagaaggtg cataataaag
     3721 cttgagagct ttataacaga aaaagatatc aatattgagg caaattttga agaagaggaa
     3781 atgtacgcga aagcagatat agattcaatt gaaagagtgg ttataaacct tttgcacaat
     3841 gccatcaaat ttgttcaacc gggcggaaag ataacactgt ctacgtcaag gagcaagaac
     3901 aaaatactgg tatgtgttga ggataacgga ataggaatag acagtaatga aattgacctt
     3961 atttgggaaa gattttataa gtcagataaa tcaagaagca aggagaaaac cggaacgggt
     4021 ctcggacttg ccatagtaag aaacataatc aatgaccacc ggcaaaagat atgggtggag
     4081 agtcaggtgg gaaaaggaac caggttctat tttaccttgg atagcggaac caacggtgaa
     4141 aacatataat tctttttatt attaacagtt tattcataag tttttcaaat tcattaaata
     4201 taataagtat aacaaaagaa aatcggttgt ttcgtgaaag gagtgttgag tatggaagat
     4261 aataattaca acggcttaaa caattatgat gaaataatta atgagcaaaa ggataatggt
     4321 acagtagaac taaacaacga taccggaaca aataacacgg ataacagcta tagctcagat
     4381 gtttcatccg gtatggaaaa cactcaaaat tatgaaaatg cttatggaac taaaaaggaa
     4441 agtgaaacgg taaacaagga tttatcgtct tcaccgtatt ataccgagag ctgtaaaaag
     4501 cctaaaaagg aaaagaaggg tgttttgctt cagatgatag tagttgccct gatgagttct
     4561 attattggag gttcggtggt gggaagcttc ttcatgttcg gcattcccgc actgaaatcc
     4621 ccggtacaat caattttagg caatggggca gtcacaggaa acgaccctga tgtaagcaaa
     4681 aatgagtcgg acatttacag gaaagtggtt attgaaagtg ctgattctcc agtggttgca
     4741 attgcagaga aggtagggcc ttcagtggtc ggtatcagtg tcaagtcaac atcagttaat
     4801 gacttctggt tcttttcacc aagcgagtcc gaagaacagg gttcaggcat aataatcaga
     4861 agtgatggat atatcatgac aaacaaccat gttattgaaa aagctttgag cggcacaaca
     4921 aacaatctgg ttcagaatgc aaaaatccag gtaatattgc cgagcaaccc ggataaaccc
     4981 tatgatgcaa cggtagtggg aagagataca aaaacagacc tggcggtttt aaagattgat
     5041 gccagcagtt tgccggctgt tgaatttgga aattctgatg atgtaaaggt aggagaactg
     5101 gcagttgcca tcggtaatcc cggcggactt gagtacatgg gttcggttac tgttggcgta
     5161 ataagcggac tcaacaggac aatacctatt accgatgaca aggaactgaa actgattcag
     5221 accgatgcct cgataaaccc gggtaacagc ggtggtgctc ttgtcaatgc cgaggggaaa
     5281 cttatagggg tgaacactgc aaagattggc gggattgatt ttgaaggcct tggatttgca
     5341 atacctgtga acaaggcaaa ggagataacc gacagtctta ttgagttcaa gtatgttaaa
     5401 ggaagaccat ctttaggcat acaggttaat accgggttta ctaaagaagt agctgaccgc
     5461 ttcggtattc cggaaggcat actcgtatac aaggttgaaa tattcagtgc agcttacaaa
     5521 gccgatattc aggaggacga tgttattacc gagttcaatg gtgtaagggt taaaaactat
     5581 gatgaattgg aagaacaaaa gagcaaattc aagcccggag atacggttaa aattaaaatt
     5641 tatagatacc atcaggaagg aaagggagag gaacttacgg tagatgttgt tatggacgaa
     5701 caaaaataag atttataatt aaataagtta cttttaaaaa cggagggata tttaaaaaat
     5761 atccctccgt ttaggttatt taagttaaga aaaatatgga aggaacatta tgaggctaga
     5821 catagccttt ttttattatt ttttatgcag agatttatta aaatattatg aggttttgcc
     5881 gatatataga ttaagaattg atatttaatg atatttaata tgttttgcga aaaaatcata
     5941 ttcctatgtt aagattttca caaaacctat atgtcaatga actttgtttt atctgaagta
     6001 tattacctgt tgtggaggta atttatgccg gaaaaatccg ttataccgat tattaaactc
     6061 atatcaatat ttctaatact aatacttttg attgtccaat tcataagtgc cccgaagctt
     6121 attacgggtt tggttactgt acttgttttg ctgtatttta ttactgacta tttgacgaga
     6181 actttttcag aacataataa taccaatgca ttgtcagatc tgaataaaaa agctataaag
     6241 ctcgaagaag aattggagtc cctacatgag gtgtcaaaaa taatcacatc tacttttgag
     6301 gtcaagacca ttattgaata tatatataat gtattgaaaa gggttacagg ttgtgaatgg
     6361 tgtcacgctt attttattga taaaaagtcg aaaatacctg taataaacta cgtgtacggt
     6421 aataaaaatt ttgccgatat aggcaattta gactatacta aatatgttca tacacttatt
     6481 gatattaatt ttagtgacga tacgaaaatt gaaattcttt ctgacaaaag cctgggcaag
     6541 attgttgcaa tttcattaaa tgtgtcgaat gatacaacgg gtgcagtttt tattgctaat
     6601 ccaactgaag gtacgttttc ggcacccaat tttaattttc ttaaaagtat tgcgtgttat
     6661 gcagccatag gtattagaaa tacagaaatg tttaacaata tttatagtca aaaagaggag
     6721 atagaagcac tttatgaaca gtctgccgca aacaatgagg aactgaacag ttatatcaaa
     6781 gagctggatg aaacaaaaga ggaattaaac agaaaaaacg aggaactgac caggctcttt
     6841 gatgagatgc aattgggcta tttacagacg gttatgtcac tggccaattc aattgaggca
     6901 aaggatccct acaccagggg acattgtcag agggttatgg aaatatcatg cgagttggca
     6961 aaggcactga aactcaccga taaggagatt gaagatctaa ggtatgcctc aatattgcat
     7021 gacattggaa agattggtat atcagcgagt attctgaaca aggaaggcaa gcttaccgat
     7081 gaagaattcg atgagattaa gaaacatcct gtaattgcat ataatatact aaaggatgta
     7141 gagtttataa aaagcggatt gaacggtata ttgcagcacc atgaaaggta tgacggaaga
     7201 ggttatcccc atggaatcaa ggggaaggaa atttgtattt ttgctaggat aatgtgtgta
     7261 gccgatgctt ttgatgccat gaccagcgac agaccataca gaaaaggcat gtcgatggag
     7321 aatgcactta aggaaataga gaggtgtaaa ggaactcagt ttgaccctga aattgctgat
     7381 ttgctcatag caatggcaag cagcggcatt tatatcggtt aaccggtata atgtttttat
     7441 tgagaaaaaa ataatactgt atcgtggaaa tgtgtttcca aatcagatat tttgggcatt
     7501 attattttag ttaaggttac catataaaac tattgaaaat atatcttatg tatgttaatt
     7561 taatttaaac tggatatgta gtgctatttc acggagtgaa atagcaaaaa gcgaaggggc
     7621 tcgaagccca tgagcttttg aaaagagtgc tatttcacgg agtgaaatag caaaaagcga
     7681 aggggctcga agcccatgag cttttgaaaa gagtgctatt tcacggagtg aaatagcaaa
     7741 aagcgaaggg gctcgaagcc catgagcttt tgaaaagagt gctatttcac ggagtgaaat
     7801 agcaaaaagc gaaggggctc gaagcccatg agcttttgaa aagagtgcta tttcacggag
     7861 tgaaatagca aaaagcgaag gggctcgaag cccatgag
//
LOCUS       DAOOJC010000050         7891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_7809_length_7891_cov_2.915518, whole genome shotgun sequence.
ACCESSION   DAOOJC010000050 DAOOJC010000000
VERSION     DAOOJC010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7809_length_7891_cov_2.915518"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLA01_02460"
     CDS             complement(<1..361)
                     /locus_tag="PLA01_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD00194.1"
                     /translation="MNHSIETLNNIVSNIEKVIVGKRKAIELILMCLICDGHALIEDV
                     PGVGKTSIVSSLAKSINASFKRIQFTPDILPSDITGFTMYNQKEGTFEYRPGSVMSQI
                     ILADEINRTSPKTQASLL"
     gene            complement(400..1350)
                     /locus_tag="PLA01_02465"
     CDS             complement(400..1350)
                     /locus_tag="PLA01_02465"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003521247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HPD00195.1"
                     /translation="MENNIIKKITIIGTGFVGSTTAYTLMLSGLISEIVLIDMNEKKA
                     DGEVMDLNHGMPFVRPVNIYRGDYRDCAGSDIVIITAGANQKEGETRIDLVKKNTAIF
                     KNIVKEIIKYNNNCILLVVTNPVDILTYVTYKISGFPKNKVIGSGTVLDTARFRYLLS
                     EHVGVDVRNVHSYIIGEHGDTEVAAWSLSNIAGIPMDKFCNDCHKCEKQISRQKIYES
                     VKNAAYEIISNKGATYYAVALAVRRIVEAIVRNENSILTVSSLLEGQYDISDICLSVP
                     AIVNKNGIDKILRVPLSDEEFNLLHKSSDTLKSIVQTLDI"
     gene            1557..2804
                     /locus_tag="PLA01_02470"
     CDS             1557..2804
                     /locus_tag="PLA01_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HPD00196.1"
                     /translation="MDAEILAVGTELLMGQITNTNAQYISQKLNSIGVNVYYHSVVGD
                     NFRRLKESLLRVLDRCDLVVITGGLGPTQDDLTKEAVSDVLEKKLVLHEESLKRIKNY
                     FNRIKRNMTDNNIKQAYLPEGCIVIANNNGTAPGCIVERDGKIVVMLPGPPKEMKPML
                     DDTVIPYLDQKSGYKTVSKYLRVFGIGESQLEDTIMDLVDTQKEVTIATYAKEGQVTV
                     RLTSKSKTYEEAMMSIRPLEEEIANRLKDKLYSTEDEELEYVASKLLIENGITVAVAE
                     SCTGGMISSRLTDVPGISKVFNRGIVSYSNESKMENLGVSPETLKKFGAVSRETAVEM
                     AEGIRRVAQTDLGISVTGIAGPDGGTDEKPVGLVYVALSDKSGCDCKELRLAGSRDKI
                     RNMTVLNVFDMIRRYVRETVNNK"
     gene            2829..4412
                     /gene="murJ"
                     /locus_tag="PLA01_02475"
     CDS             2829..4412
                     /gene="murJ"
                     /locus_tag="PLA01_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPD00197.1"
                     /translation="MGSNKKLTGAALIVMSSIVVSRITGFLREMLVPNMIGVNEAGDA
                     YTIAFRITGLMYDMLVGGAISAALIPILSGYIAKKDEENGWKAVGTFVNAVIVAMSVV
                     CLSGIIFAPQLVSLIAVGFDSEAQKQLTIDLTRILFPSVGFLMLAGLSNGVLNSYHRF
                     AAAAYGPSIYNIGSALSILLFSKSRWGVRGVAFGVMGSAIIYFLFQLSFAFRNLRYYR
                     FKFYLKHPGFFRLLRLAIPSLISSAVVQINAVITGTFATLFGVGGATALNIADRTWQM
                     PYGIFAQGMGIAMLPTLSSNIATGKIDEYKDTLMKGIKTVLLLTVPSGVGFIVLREPI
                     IRTIFKFTSLFDEEAVMVAGSILMFFSIALLSQSIVTVVNRAFYAINDTLTPLLIGGS
                     TIIINIALSVIFYQTTDLGVAGMALAYSLASALNAFLLLTVLNKKMKGIYLLNLLKFL
                     LRVVPASIIMGGVLFLINIFIDMDTGSKLMQVLGLTLEIAAGVLIYFTAVLLMRVEEA
                     TYVKNRFVSKIKGFVGKKA"
     gene            4537..5577
                     /gene="recA"
                     /locus_tag="PLA01_02480"
     CDS             4537..5577
                     /gene="recA"
                     /locus_tag="PLA01_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPD00198.1"
                     /translation="MIEKKKALEIALSQIEKQFGKGAVMKLGENTHMNIETIPTGALG
                     LDIALGVGGVPRGRVVEIFGPESSGKTTVALHIIAEAQKAGGEAAFIDAEHALDPVYA
                     KNLGVNIDLLIVSQPDTGEQALEIAEALVRSGAIDVIVIDSVAALVPKAEIDGEMGDA
                     HVGLQARLMSQALRKLSGVINKSKTTAIFINQLREKVGVMFGNPETTPGGRALKFYAS
                     VRLDVRRIESIKQGNEVIGNRTRVKVVKNKVAPPFKEAEFDIMYGQGISKEGNTLDVA
                     VNLDIINKSGAWYSYNDQKIGQGRENTKQFLRDNPDILREIEGKIRENYNQAFLMSVD
                     VHSDNDDEQLDD"
     gene            5891..6511
                     /locus_tag="PLA01_02485"
     CDS             5891..6511
                     /locus_tag="PLA01_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HPD00199.1"
                     /translation="MVITSVEKSKKNKGMFSVYVDDRYAFSIPEEDYLRLCLYEKKEI
                     SDEEIEFIRKNVTFKSAKSQAVKYLSYRLITEAQLFDKLQGKGFDEDTILDVISELKS
                     MGYINDLIYAQKYVYERIKLKPKSKKMLKMELKSKGVSEDIADQVLNEMEIDEDIVID
                     RLIKKKFGKYNIKEPQIEKKIYYFLLHRGFSMENIRTALNRITNSI"
     gene            7501..>7891
                     /locus_tag="PLA01_02490"
     CDS             7501..>7891
                     /locus_tag="PLA01_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00200.1"
                     /translation="MKKINALVCVFLLLLGILGITTINAAADGGIGGYSWQKISGNYL
                     GTTEVKSSADSLEISFQNINYDNNAAGASTTSAGEGAIPLDEDWTKYEAVNFTITNPS
                     SAELQVGLGICTGPNWYWHESPTTSVPT"
BASE COUNT         2449 a         1244 c         1749 g         2449 t
ORIGIN      
        1 ccaaaaggct tgcttgtgtt ttgggtgaag ttctgtttat ctcgtcggca aggattatct
       61 gactcatgac actgccggga cggtattcga aagtaccttc cttttgattg tacatcgtaa
      121 agcctgttat gtctgaggga agaatatccg gagtgaactg tattctttta aacgaggcat
      181 tgatggattt ggcaagggat gacacaatgc tggttttacc tactcccggt acgtcttcaa
      241 taagtgcatg gccgtcacag attaagcaca tgagtataag ctcaatagct tttctttttc
      301 ccacgataac cttttcaata ttcgatacga tattgtttaa ggtttcaatt gaatggttca
      361 taagtactcc cccttatttt tcagttttag gaaaacaaat catatatcaa gagtctgtac
      421 tatacttttt aatgtatcgg acgatttatg cagtaaattg aattcttcat cgcttaacgg
      481 taccctgaga attttgtcta tgccgttttt gttgacaatt gcaggaacgc tcagacagat
      541 atcacttatg tcatattgcc cttccaagag actggacacc gtaagaatag agttttcatt
      601 ccgcacaatg gcctcaacga ttcttctgac tgcaagggct acagcataat aagtggcacc
      661 tttgttgcta ataatttcat aggcagcatt ttttacgctt tcgtatattt tttgccttga
      721 aatctgtttt tcgcatttat gacaatcatt acagaacttg tccatgggaa ttcctgcgat
      781 gtttgaaagg ctccatgcgg caacttccgt atctccatgc tcacctatta tatatgaatg
      841 aacgtttctt acgtctacgc ccacatgttc gcttaagaga taccggaatc tggcagtgtc
      901 caataccgtt cccgagccaa taactttgtt tttcgggaaa ccggatattt tgtaagtaac
      961 ataggttaat atgtctacag ggttggttac tactaaaaga atgcaattat tattgtattt
     1021 tataatttct tttacaatgt ttttaaaaat tgcggtattc ttttttacaa gatcgattcg
     1081 tgtttctcct tctttctgat tagcccccgc ggttataatt actatatccg atccggcaca
     1141 atccctataa tctccgcggt atatattgac aggtcttaca aagggcatac cgtggttgag
     1201 atccatgact tctccatcag cctttttttc gttcatgtca atgagaacaa tttcggaaat
     1261 caacccgctg agcattaatg tataagcagt tgtagaaccg acaaaaccgg ttccgataat
     1321 tgttattttc ttaattatat tgttttccat gttaaagact cctttatttc cattttcaac
     1381 agttatatta tacccctcaa ctgtatataa gacaatatat gacgtgtaga ataatgtggc
     1441 tatatcggtt ggtttatgtg aatggcatat gaaatattaa tcattaaatt acttttattt
     1501 taatatcaat attgatataa taggttacaa aaagtttaaa attaggtgat gatattatgg
     1561 atgcagaaat attggcagtt ggaacagagc ttttgatggg gcaaataaca aatacgaatg
     1621 cccagtatat atcacaaaag ttaaacagta tcggggtcaa tgtatattat cacagtgtgg
     1681 tgggagataa tttcaggagg cttaaagaaa gccttttaag ggtattggac agatgcgatc
     1741 ttgttgttat aaccggcggt ctcggaccta cccaggatga ccttaccaaa gaggctgttt
     1801 cggatgtgct tgagaaaaaa ctggttttgc acgaggaaag ccttaaaaga atcaagaatt
     1861 attttaacag aataaaaagg aatatgaccg acaataatat caaacaggct tatttgcctg
     1921 aaggatgtat agtaattgca aacaataacg gtacggctcc gggatgtatt gttgagagag
     1981 acggcaagat tgtcgtgatg cttccgggac ctccgaagga aatgaagccc atgttggatg
     2041 atactgtcat accttacctg gatcaaaaat ccggctataa aaccgtatca aaatatttaa
     2101 gagtgtttgg aatcggtgaa tcacagcttg aagacaccat aatggatttg gttgacacac
     2161 agaaagaggt gacaatagct acatatgcaa aggagggcca ggtgacggta aggcttacat
     2221 cgaaatctaa aacctatgag gaagcgatga tgagtataag gcctttggaa gaagagattg
     2281 caaacaggct gaaagacaag ctgtacagta cggaagatga ggaactggaa tatgttgcat
     2341 caaagctgct tatcgaaaac ggcattacag ttgctgtagc agagtcttgt acgggaggaa
     2401 tgatttcatc gaggctaacg gatgtacccg gtatatcaaa ggtttttaac cggggaatcg
     2461 tttcttacag caatgagtca aagatggaaa acctcggtgt ttcacccgaa acactaaaaa
     2521 aatttggggc cgtaagccgt gagactgcag ttgaaatggc tgagggtata agaagagtgg
     2581 cacaaacgga tctgggcatc tcggttacag gtattgccgg tcctgacgga ggtactgacg
     2641 aaaaacctgt cggtcttgtc tatgtggctt tgtcggataa atccgggtgt gattgcaaag
     2701 aactgcggct tgcgggaagc agggataaaa taaggaatat gacggttctg aacgttttcg
     2761 atatgataag aagatatgtt agggagacgg ttaataataa ataaaaactg accgggagga
     2821 tttacaaatt gggaagcaat aaaaagctga cgggtgctgc acttattgta atgtcatcca
     2881 tagttgtaag cagaataacc ggttttttaa gggaaatgct tgttccgaac atgattgggg
     2941 taaacgaagc aggggatgct tataccattg ctttcaggat aaccggtttg atgtatgata
     3001 tgcttgtagg gggagcaatt tctgctgccc ttattccgat actttcgggt tatatagcaa
     3061 aaaaagatga agaaaacgga tggaaagcgg tgggcacatt tgtaaatgcg gttattgttg
     3121 ccatgtctgt ggtatgctta tcaggaatca tatttgcacc acagctggtc tcgctgattg
     3181 cagtggggtt tgatagtgaa gcccaaaaac agcttaccat agatctgacc cgaatactct
     3241 ttccgtcggt gggattttta atgctggcag gtctttcaaa cggggtttta aattcttacc
     3301 acaggtttgc ggctgcggcc tatggcccat cgatatacaa tataggaagt gccttaagca
     3361 tattgttatt cagcaaaagc agatggggcg taaggggtgt tgccttcgga gtaatgggga
     3421 gtgcaataat ctacttttta tttcaattat cctttgcttt cagaaatctt aggtactaca
     3481 ggtttaaatt ttatttgaag catccgggct tttttaggtt attaaggctt gcaattcctt
     3541 ctttgatttc ctcggcggtg gttcagatca atgctgtcat aaccggtacc tttgcaacgc
     3601 tttttggcgt tggaggagcg acagctctta acattgcaga ccggacatgg cagatgccat
     3661 acggtatttt tgcccagggg atgggcattg ccatgcttcc tacattgtca tcaaatattg
     3721 caacgggcaa gattgatgag tataaagata ctcttatgaa gggaataaaa accgttctcc
     3781 tcttgactgt accgtcgggt gttggcttta ttgtgctaag agagccgata ataagaacga
     3841 tattcaaatt tacaagcctc tttgacgagg aagctgttat ggtggcggga agtattctga
     3901 tgtttttttc catagcactt ttaagccagt ccattgttac tgtcgtgaac agggctttct
     3961 atgcaataaa tgacaccctg actcctcttt tgattggagg aagtaccatt attattaata
     4021 ttgcattaag cgttatattt tatcaaacaa cggatttggg tgttgcgggt atggcactgg
     4081 catattctct tgcgagtgca ttaaatgctt ttttacttct gaccgtatta aacaaaaaaa
     4141 tgaaaggtat atacctttta aaccttttga agtttttgct tagggttgtt ccggcttcca
     4201 taattatggg aggtgtactt ttcttaatta atattttcat tgatatggat acaggttcaa
     4261 agctcatgca ggttttaggc ttaacccttg aaatagccgc gggagtactg atttatttta
     4321 cagctgttct tttaatgagg gttgaagagg caacctatgt aaagaacagg tttgtttcga
     4381 agattaaggg ctttgtagga aaaaaagctt agtatttcaa tgtcttgtga cggaaattaa
     4441 aatttctacc cagataattc aaaagttgtt gactatatga ttgcgttatt gtataataaa
     4501 aaagaacgca tgttcgatgt ataggaggat gaaaaaatga ttgagaagaa aaaagctctg
     4561 gagattgcat tgagccagat tgaaaaacag tttggaaaag gtgctgtaat gaagctgggt
     4621 gagaacaccc acatgaatat tgagaccatt cccacaggtg cattgggatt ggatatagca
     4681 cttggggttg gcggagttcc aaggggaagg gtagtagaaa tattcgggcc tgaatcctca
     4741 ggtaagacaa cagttgcttt gcatattatt gccgaagctc aaaaagcggg tggtgaagca
     4801 gcatttatag atgctgagca tgcactggac cctgtgtatg caaaaaatct tggcgttaat
     4861 atagatctcc ttattgtttc ccagcctgat acaggagagc aggctttaga gattgcagag
     4921 gcattggtta gaagcggagc tattgatgtt attgtaattg actctgttgc cgcattggtt
     4981 ccgaaggctg aaatagacgg tgaaatgggt gatgcccatg tggggcttca ggcaaggcta
     5041 atgtcccaag ctcttagaaa gctttccggt gtcattaaca aatcaaagac aactgctatt
     5101 ttcataaacc agctgagaga aaaagtcggg gtaatgtttg gaaaccctga aaccactccc
     5161 ggaggacggg cactgaaatt ttatgcatcg gtaaggctgg atgtcagaag gattgagtcc
     5221 atcaaacagg gaaatgaggt tattggaaac aggacaaggg ttaaagttgt gaagaacaag
     5281 gttgcacctc cttttaaaga agcggaattc gatataatgt acggtcaggg aatttcaaaa
     5341 gaggggaata ccttggatgt tgctgttaat ctggatataa ttaataagag cggggcatgg
     5401 tattcctata acgatcagaa gattggacag ggaagggaga acacaaagca attcttaagg
     5461 gacaaccccg atatattaag ggagattgag ggaaagatca gagaaaatta taaccaggca
     5521 tttttgatga gtgttgatgt acattcggat aatgatgatg agcaattgga tgattaagtc
     5581 tatcttgcgg tgtgatctca aaattaccaa tcggagcaag aatatttgga aacttgacgc
     5641 ttcaacgaat tcgcaggatt ttcattgagt aaatacctaa gaaactctat actggaagat
     5701 aggggtgcaa gatacgcacc tcttattttt atcattatct tatgaagaga aatgagcaaa
     5761 aagcagagga ctcaatgttc ctaagttttc aatgctattt tatggatgtt atttttgtag
     5821 tataataaag atagaaggat aatattaata actaattata aattaaaagt aaattttaca
     5881 ggtgattgat atggtaatta cctcagtgga gaagagtaaa aagaataagg gtatgttttc
     5941 tgtttatgtt gatgacagat atgctttttc aatcccggaa gaagactatc ttcgcctttg
     6001 cctttatgaa aaaaaagaaa tatctgatga ggaaattgag tttatccgga agaatgttac
     6061 atttaaaagt gcaaagtctc aggctgttaa atacttatcc tataggctta taaccgaggc
     6121 acagcttttt gataagttac agggcaaggg ttttgatgag gatactattt tagatgttat
     6181 aagtgaactc aaatccatgg gttatataaa tgatttgata tatgctcaaa agtatgttta
     6241 tgaaagaatt aagctaaaac ccaaatccaa aaagatgctc aaaatggagc ttaaaagtaa
     6301 gggtgtctca gaagatattg ctgaccaggt attaaatgag atggagattg atgaagatat
     6361 agtaattgac agattgatta aaaagaaatt tggcaagtat aatataaagg agcctcaaat
     6421 agagaaaaaa atatactact ttttgttgca tagggggttc agtatggaaa acataagaac
     6481 cgcattaaac agaataacca attcgatttg acagtcttat tacacttcat ttaaaagtgt
     6541 cacaagatag atcgcccact gaaaggcgaa tcggtatttg cagcttattg caggtgtgga
     6601 acatctattg ccttattata tgtaaacgtt gggaatcgat gttttttgcg gctttgcgaa
     6661 gaaataacat ctattgcctt gttatatgta aatcatataa attgggttaa tcagcgtgtt
     6721 taattaaata tgaatacagg aactttgttg ataatattcc aaaaagaagt taagaagagg
     6781 agctttaact tataaaaggc agggctcttt ttttatgtat tcacgatcaa ttaataaagt
     6841 tatttgtata gaaatattat tacaatatca taattgtcac tttacatatt tctaaaaaca
     6901 tgatatatta ttatttagag ataaagcatg aatgagataa tcctttgtca ggaagtgcta
     6961 atcatactta atataaaatg aattgcataa gttcttatat tgaggattta gtgccgttca
     7021 tatagtgttg aatattagaa tttcaggggc ataatagaaa atggggaggg atgtttcaac
     7081 attatcatat ttgtttgtat ttgaggggga tgtgggtatt tacaggtaat ttaagtaaca
     7141 atgcgattgt aatgatattg aagtcgaagt ttgtatttac atattcatgc attttaatat
     7201 tactcaatat accaatctat tcagtttatt attctaagat ataaagtgtt caacttatct
     7261 gctggtatag tgtttttgga atttccattc aaataaaatc ctccgaaacc attaaaccat
     7321 cgggattctc gaatttttat tatgcgtaag taatttgagg atacacagct agtttttatt
     7381 taaagattgc catttttaaa atgcaattgg ccgataagct tttattttat tatatcaagg
     7441 ttttagttta ttttgaccgt tgcatattga atttattatt atttaggagg ttgaggagta
     7501 atgaaaaaaa ttaatgctct tgtatgtgtt tttttattgt tgctcggaat tttggggatt
     7561 acgacaataa atgctgcagc ggatggagga attggaggtt actcatggca aaaaatctca
     7621 ggcaattatt tgggcacaac tgaggtaaag tcatcggctg acagtcttga gataagtttt
     7681 cagaatataa attatgataa taacgctgca ggtgcttcaa ccacatcagc aggagaagga
     7741 gctatacccc ttgatgagga ctggacaaag tacgaagcag tgaatttcac aattaccaac
     7801 ccatcatctg cggaacttca agttggtctt ggaatttgta ccggacccaa ttggtattgg
     7861 catgagtcac caacaacttc agtgcctacg g
//
LOCUS       DAOOJC010000051         7890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_7810_length_7890_cov_4.502999, whole genome shotgun sequence.
ACCESSION   DAOOJC010000051 DAOOJC010000000
VERSION     DAOOJC010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7810_length_7890_cov_4.502999"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..423)
                     /locus_tag="PLA01_02495"
     CDS             complement(88..423)
                     /locus_tag="PLA01_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPD00201.1"
                     /translation="MEYYLEYARIFKALGDPRRAMIVDMLSCGELCACMILEKFEMSQ
                     STLSHHMKLLCECGLVKGREQGKWTYYSLDRNTVSKIKRFICDITSDKGNYICKEDTI
                     CCKGCDENE"
     gene            548..841
                     /locus_tag="PLA01_02500"
     CDS             548..841
                     /locus_tag="PLA01_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPD00202.1"
                     /translation="MKKNLFMHDLFKGWTIDTDSQFKLDYTKPAPFADDNFISDWAKD
                     SVYSMVAKDIIKGIGGNKFAPKNTTTAEETSNFSNATREQALIIAAKIVENLK"
     gene            complement(929..2110)
                     /locus_tag="PLA01_02505"
     CDS             complement(929..2110)
                     /locus_tag="PLA01_02505"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HPD00203.1"
                     /translation="MYEKKLLWIVMAIIVTILLAACDIGKDENSSPTDNPEPIKTETD
                     VSQNDEQDATSAPITAELLGFEGGNIGAGGLVCNGKDGYIYYRSEGDGWKLYKAKPDG
                     SGKTKLSDRPPEYINVLGDWVYFCDYTDDFSIYKVRTDGTDEIKLVDGYCNNLYVAEG
                     GMYFDMRDENNSPQIYHTDLDGKNMTKLVPDASLMYYYKDKIYLGARQLKVYDIKTGK
                     EKVLVETYIHNVSVDDSGIYFWAADKGEFHRLDLDGDNDTIILRGGDYFNYSGGNLYY
                     TGISENKNGPCHTVNCLNIATDKTVTLLEEANEYFNAHGEWVGVTFKQFNEHPETIDP
                     KLLKQNEAGEIFVGYNESVGYVYVSGEYLYTRASLRESILVNGKLDCIAKIDDGIMIW
                     D"
     gene            complement(2315..3229)
                     /locus_tag="PLA01_02510"
     CDS             complement(2315..3229)
                     /locus_tag="PLA01_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD00204.1"
                     /translation="MDSLIIKNILKDCVLCPRECHVDRTAGKRGYCKTTGELVVARAA
                     LHMWEETCISGEEGSGTVFFSGCALGCVYCQNRNISKGLAGSNITIERLAEIFLELQG
                     KGANNINLVTPSHYVPHIIEALNISRRKGLILPIVYNCSGYEKVETLKLLEGCVDIFL
                     PDLKYMSYEMAMKYSNCKNYFTVAAAAIEEMVRQVQETSFDERGIMQKGVIVRHLTLP
                     GCLEDSKRIIKYLYETYGDRIFISIMNQYTPITKNRVYPELKRKITEKEYEELVDFAI
                     EIGLENGFIQEGETASESFIPEFNGEGV"
     gene            complement(3681..5027)
                     /locus_tag="PLA01_02515"
     CDS             complement(3681..5027)
                     /locus_tag="PLA01_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00205.1"
                     /translation="MDHNVINLELGTKMIEGPVTVFVNNSGLDKSYAIVDYAGYKVFK
                     TSRTFDYVKDTSVPVVTVKSANHDKVVLGFSKPVKGTNIKLYHTVINDESREATATET
                     DYTDEITFTYDKKTKDGLRSRSVKLFLVNSQAEDEKLVDIDYIYVPDQSLVCEVEETP
                     NPNPTPTPTRKASTHTKTPAQTPAQTPAQTPTQTPTHTPTNPPTNPPTNPPTTPPTAT
                     ATPVVDQTCPELVTDPQNVQYCCTVPEEGFIYIVFSEAMNEDQMLDKENYQVSIDEGE
                     NYITLGDDAITKVNESTVRIYFEDIKDKNIEFGVIIAPWVKIAPITDLAGNKLYGSDE
                     PYILESIDPEYVCINEAYLIASNKIKIIFNKEMETVELSDIVIQLTTQDLVYATGCES
                     NTVNFLGQTEVVLELPIDEEDGEGEPIYIYTAEITSSVSKWGSKLTPGDSCMINNE"
     gene            complement(5071..6213)
                     /locus_tag="PLA01_02520"
     CDS             complement(5071..6213)
                     /locus_tag="PLA01_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00206.1"
                     /translation="MRRLRRFAAALLTVVLILTSMPAVFADTSATIENADRAAALKDL
                     GLYEGQDPNDPAYGLENALTTQDSLIFLAKLFGYNDAANALTQDQVADALAKFDDAAS
                     ISEYARSVVAYSAANDILCGSIQDGKLFVGAKDTVTAARFATFMLKQMGYTVEDYKVS
                     VAKLAETKGSNIDATLNGDLTRDDAVGVMYGALTAEKASRKTVIADIIGDNADLKKKA
                     EKIGLLDDTPVDTMDLVVESVNVLNCKQIEIKFNQEMNRRSVESAEFYEIYDKGEDEG
                     KIELSDNSISLDDDKKTVIITLNKNVSDKLTNSSKAKVIVKKKIRAVSGSKLAEDAVF
                     DKVYVEDGIIPTIIKAEAKGERLIRITFSEPVYDNDNDNSISVDKL"
     gene            complement(6575..7114)
                     /locus_tag="PLA01_02525"
                     /pseudo
     CDS             complement(6575..7114)
                     /locus_tag="PLA01_02525"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
     gene            complement(7333..>7890)
                     /locus_tag="PLA01_02530"
     CDS             complement(7333..>7890)
                     /locus_tag="PLA01_02530"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPD00207.1"
                     /translation="EEIGMALKNKSIYAILGILNLSPCSGYDIKKYSDKVLSGFWNEN
                     FGHIYPTLKMMLEDGLIEIVSREENQNKIKYGITEKGKQELEAWLLEETVPQPIRSEF
                     LLKLLFSSSQPRDKVIHMLESYKKLHEKDLDKYLEMQKNLEQDNRDISKERVCFMKAV
                     LRRGVISSEAVIHWCDETIEELQRS"
BASE COUNT         2252 a         1776 c         1235 g         2627 t
ORIGIN      
        1 ccaattaccc attcatcatt tttatcatac ttcgtgatgg attccatgct gcaacaacca
       61 ctagccgaac agcaacagga cttctcgtta ctcattttca tcacatcctt tacaacagat
      121 tgtatcttct ttgcagatgt aattgccttt atcagaagta atatcgcaga taaaccgctt
      181 tattttgctg acagtatttc tgtccaatga gtaatacgtc cacttacctt gttctctgcc
      241 cttgaccagc ccacattcac acaagagctt catgtggtgg gacaacgtag attgggacat
      301 ctcgaatttc tctaaaatca tacaagcaca gagctcccca caggagagca tatctacaat
      361 catggctctt ctagggtccc ccagtgcctt gaatattcta gcatattcca agtagtactc
      421 catatcctca cctcaaatct ataaatatcg atatgactat tatatatgtc aaatcgaaaa
      481 acgtcaatat gaaggcataa aattatacag tatatcagta attaaatgta agaagattac
      541 aaactgtttg aaaaagaatt tattcatgca tgaccttttt aaaggctgga ccattgatac
      601 agacagccag ttcaagctgg actataccaa acctgctcct tttgccgacg acaattttat
      661 ttcagactgg gcaaaggaca gcgtatactc tatggttgcc aaggatatca tcaaaggtat
      721 tggcggaaat aagtttgctc caaagaatac cactactgca gaagagactt caaatttctc
      781 caatgcaact cgtgagcaag cacttattat agctgcaaaa atcgttgaaa acctgaagta
      841 aagcttattt attaaaaggc ggaagggtgt atcccttccg ctttgttttc ccaagtattt
      901 aataagtcga tttataaaac aatgcagttc aatcccaaat cattattccg tcatcgattt
      961 tggcgataca gtccagtttt ccattcacaa gaatactctc acgcagtgaa gcacgtgtat
     1021 agagatattc tcctgaaaca taaacgtatc ccaccgattc attatagccg acaaatatct
     1081 ctcctgcctc attctgttta agaagcttag gatcgattgt ttccggatgc tcattaaact
     1141 gtttgaacgt aacaccaacc cattcgccat gtgcattaaa atattcgttt gcttcctcca
     1201 gtaaagtcac tgttttgtct gtagctatat ttaagcagtt taccgtatgg catggaccgt
     1261 ttttgttttc actgatgcct gtatagtaga ggtttccgcc actataattg aagtaatcgc
     1321 cgccgcgtag aattattgtg tcattgtcac cgtccaaatc caagcggtga aattctccct
     1381 tgtctgcagc ccaaaaataa ataccgctgt catcaacgga aacattatga atatatgtct
     1441 cgacaagtac tttctctttt cctgtcttaa tgtcatatac tttaagctgc cttgctccca
     1501 gataaatctt gtctttatag tagtacatta aagatgcgtc cggcacgagc tttgtcatat
     1561 tcttaccgtc taagtcggta tggtagattt gcggtgaatt attctcatca cgcatgtcaa
     1621 agtacattcc accttcagca acatagagat tgttgcagta tccatcgaca agttttattt
     1681 catctgtgcc atcagttctt actttataaa ttgaaaaatc atcggtataa tcacaaaaat
     1741 atacccagtc tccaagaaca ttgatatatt ccggcggacg gtcactaagt tttgtttttc
     1801 cggagccgtc aggttttgcc ttatacagct tccaaccgtc gccttcgctg cgataataga
     1861 tgtagccgtc cttcccatta caaaccaagc ctcctgcacc tatgttaccg ccctcaaagc
     1921 cgagtagttc cgcagtaatg ggtgcacttg tggcatcttg ttcatcgttt tgtgatacgt
     1981 ctgtttccgt ttttattggt tctgggttgt cagtcggcga gctgttttca tctttgccta
     2041 tatcgcaagc agcaagcaaa atagtaacaa ttatcgccat tacaatccac aatagttttt
     2101 tctcgtacat ttaaataact cccttcttat caaataaaaa tttatgttaa gatatacatt
     2161 gcagctggca acactagcgt aggtttcagt aatcattcgc cattgaatct atttaaatta
     2221 taaatataaa aattgagttc taagaaggca taacttcatt ggtatattca tataaagcat
     2281 tgatatcaag ttccactgag aactgatcct gtatctatac accttccccg ttaaactccg
     2341 ggataaagct ttccgaagct gtctcccctt cttgtataaa tccgttctcc aacccgattt
     2401 ctattgcaaa atcaacaagt tcttcatact ctttttcagt tatctttctc ttaagctccg
     2461 ggtataccct attctttgtt atcggagtat attgattcat aatgctaata aatatcctgt
     2521 ctccatatgt ctcatacagg tattttataa ttctttttga gtcttcaaga catcccggca
     2581 atgtcaggtg ccttactatc acaccttttt gcattatgcc ccgctcatca aagcttgttt
     2641 cttgtacctg cctgaccatt tcttcaattg ccgctgctgc aaccgtgaaa taattcttgc
     2701 aattggagta cttcattgcc atctcatacg acatatattt taaatcgggc aaaaatatat
     2761 caacacatcc ttcaagcaat tttaaggttt ctactttctc atacccgcta caattatata
     2821 caataggaag aataagacct tttctgcgtg agatgttcaa agcctctata atatgcggaa
     2881 catagtggct tggagtaacc agattgatat tgttggctcc cttgccctga agctcaagaa
     2941 aaatttctgc aagtctttct attgtaatgt tacttcctgc caaacccttt gatatattcc
     3001 tgttctgaca atatacacac cccagagcac aaccggagaa aaaaaccgtg ccagagcctt
     3061 cttcccctga tatgcaagtt tcttcccaca tatggagtgc tgcccttgca acgactaact
     3121 caccggtagt tttgcaatat cctcttttgc cggcagttct gtccacatga cattccctgg
     3181 ggcaaagaac gcaatctttt aaaatatttt ttataattaa cgaatccatc gataatcctc
     3241 ttttcatata ctctttggta tgactataca ttgcacattt tgctctgcag gttgtacctt
     3301 tctacattca tatatgactt agtaatctga ccttttgttc cactctatat tgcgtcacaa
     3361 gatttatttc gcaaataaat tatactataa ttgagccatc tgtacacggg tgtcggtttc
     3421 attcgtcaag aacttgccgt tgctgccgca gataatgcct gcttcaacaa ggcaaaatat
     3481 agcttctcta gcccatgaag ctaaatctct ctcatcttta caccatctat aaatttagac
     3541 ttcccctact gatattctaa caatcaaaac atcattagaa aattgtgtaa ccgccaaaaa
     3601 acagctttaa taatgtgaaa tcaaatctca ggagtttatt catcattagc atgatccagc
     3661 ttagttatct cagaaggtgc ctattcatta ttaatcatgc aggaatcgcc cggtgtaagc
     3721 ttactgcccc atttcgatac tgaagatgta atttcggcag tataaatgta aatcggttca
     3781 ccctcaccgt cttcttcatc aataggcaat tctaaaacca cttccgtttg tcctaaaaaa
     3841 ttaacagtat ttgattcaca gcccgtagca taaactaaat cctgagttgt taattgaatt
     3901 acaatatcac tcaattcgac ggtttccatc tccttattga atataatctt tatcttgtta
     3961 gaagctatta aataagcttc attaatacag acatattccg gatcaatact ttccaaaatg
     4021 taaggctcat cgctaccgta caatttattt ccggcaagat ccgtgatcgg agctatcttc
     4081 acccagggag ctataatcac gccaaattct atattcttat ccttaatgtc ctcgaagtaa
     4141 attcgaactg tcgattcatt tactttggta attgcatcat cacccagtgt tatataattt
     4201 tcaccttcat caattgatac ctggtagttc tccttatcaa gcatttgatc ttcattcata
     4261 gcttcagaga atacaatgta aataaaaccc tcttcaggca ccgtacaaca atactgaacg
     4321 ttttgaggat ccgttactag ctcaggacat gtttgatcaa caacaggagt agctgtagcc
     4381 gttggaggag tagttggtgg atttgttggt ggattagtcg gaggatttgt cggagtatgc
     4441 gttggagttt gtgttggagt ttgtgctgga gtttgtgctg gagtttgtgc tggagtttta
     4501 gtatgagttg aagccttgcg ggttggagtt ggggtcgggt tcgggttcgg tgtttcttca
     4561 acctcacaaa caagagactg atccggcaca tatatgtagt caatatcaac taacttttca
     4621 tcttcagctt ggctgtttac caggaaaagt ttaacacttc tgcttcgcaa tccgtccttc
     4681 gttttcttgt catatgtaaa tgtaatctca tctgtatagt cagtctctgt agctgttgcc
     4741 tctctagatt catcgtttat tactgtatga tacaacttga tatttgtgcc cttgaccggc
     4801 ttcgaaaaac cgagaacaac tttgtcatga tttgctgatt ttacagtaac aaccggtacg
     4861 gaagtatcct taacatagtc aaatgttcta cttgtcttga atactttata tccggcatag
     4921 tcaacaatgg catatgattt atccaggccg ctgttattaa caaaaacagt tacaggacct
     4981 tcaatcattt ttgttccgag ttccaggtta ataacattat ggtccaaagt agctttctga
     5041 acgtaatatt catatgttcc gcttacaacc ttaaagttta tctacagaaa ttgaattatc
     5101 attatcgttg tcataaacag gctctgaaaa tgtgattctt ataagtcgct ctcctttagc
     5161 ctcagccttt ataattgtgg gaataatacc gtcttctaca tagactttat caaatacagc
     5221 atcttcagct aatttactgc cactaactgc tctgattttt ttcttaacta ttacttttgc
     5281 tttagatgag ttcgtcaact tatcagaaac atttttattt aaagtaataa taactgtttt
     5341 tttatcatca tctaatgaaa tactgttatc actaagttca attttcccct catcttcacc
     5401 cttatcatat atttcataga actccgcaga ttcaactgac cttctattca tttcctggtt
     5461 aaatttgatt tctatctgct tacagttcaa aacattaacc gattcaacaa ccaagtccat
     5521 agtatctaca ggggtatcat ccaataagcc aatcttctcg gcctttttct ttaagtcagc
     5581 attgtcgccg ataatatcgg caataactgt ctttctgcta gccttttcag cagttagagc
     5641 accatacata acgccaaccg catcgtctct ggttaaatca ccgtttaaag ttgcatcgat
     5701 attgcttcct ttagtttctg caagctttgc aaccgatacc ttgtaatctt caacagtata
     5761 acccatttgc ttgagcatga atgttgcaaa tctggcagct gtaacagtat ccttagcacc
     5821 tacaaacaat ttaccatcct gaattgagcc gcacaaaata tcatttgctg ctgaataggc
     5881 aactacactc ctggcatatt cagatatact tgcagcatca tcaaatttag ctaatgcatc
     5941 agctacctga tcctgtgtta aagcattagc agcatcgttg taaccaaata attttgctaa
     6001 aaaaatcaat gaatcctgtg ttgtcaatgc attctccaag ccatatgcag gatcgtttgg
     6061 atcctgacct tcatagagac ccaaatcctt taatgctgct gccctgtcag cgttttctat
     6121 cgtagctgaa gtatctgcaa atactgcggg catagaagta agaattaata ctactgtcag
     6181 aagtgctgct gcaaatcttc ttaatcttct catattactg tattctcctt tcaaatgcag
     6241 aaattaactg accaaagagt tgctaatgtc tttatcggca agatttttat atgtgattat
     6301 tctttaataa tatcacattt tttatgtaaa tcaaaaggac cgagtgtaac attattcata
     6361 aattattagt gtccattgac tcttgtactg ctttaaagct cagcatacat tacaccctta
     6421 acaccaaagc tgcacactct acaactatgg tatagtaaac cgcctgtcat aatagttttc
     6481 taatgacaaa gatttggata taattataag ttcaatcttc cggcagtagt agagcatgaa
     6541 caattaagct gaaggctttt aataagtgca ttgattaact tttaaatatc atttccgaat
     6601 atttaataat attctcgccc aatattcttg cctgactcag tctattttcc tgctttagaa
     6661 tatcgccagg cttgtctgct ctggcagata aaccattgat ttcacaaggc acacagattg
     6721 cacccgaaga taaataataa ttatgtataa tggtgagcac aaggctttgt cccccgcctt
     6781 ctcctcttcc tacagcaata gctcctccaa gttctccggc taataatttt ctgttgttga
     6841 aaacaaagct tctatcaaac agagctttca gttgtgccgt tacattccgg taatatgcag
     6901 gagaccctat aatcagagca tcacaatctt caagttcact taacaaaagc tccatatcat
     6961 caccttcaat ggcacattcc ccagtctctc tacatgtttc acaacccatg cagggattta
     7021 tcgttttact gcttaaaaag aattcagtta ctttccatcc tttttcataa aaaggcttaa
     7081 gtgcctctct tactagaatc tctgtgttcc cgtcttttct tggactgcct tatattccta
     7141 tcactttcat atatcttgct cctccctata taattctcat ttcagtaatc tgctttttta
     7201 gctgtaagcg tccattctgt ttcctcgccc cacatacaaa tatacattat ctaacctgtc
     7261 aactcaaccc taccaatttt tgagctaaaa tataacccca aaaatactcc ctccaattga
     7321 aaaaccgccg ttctaggaac gttgtaattc ttctattgtt tcatcacacc agtggataac
     7381 agcttcactg gatataacac cccttctaag gaccgctttc ataaaacaga cacgctcttt
     7441 tgatatgtcc cggttatcct gttccagatt cttttgcatt tcaagatact tatccaaatc
     7501 cttttcatgg agttttttat aactctccag catatggata actttatccc tcggctggct
     7561 gctggaaaac aacaatttta gcaaaaattc ggaacgtatc ggctgcggaa cagtttcttc
     7621 caaaagccat gcctcaagct cctgctttcc cttttctgtt attccatact ttattttatt
     7681 ttgattttct tctctggaaa cgatttcaat caacccatcc tccagcatca ttttcaatgt
     7741 gggataaata tgtccgaaat tttcattcca aaacccggat agtaccttgt cactgtactt
     7801 ttttatgtca tagccgctgc atggcgacag attcagaata cccaaaatag catatatgga
     7861 tttattcttt aatgccattc ctatctcctc
//
LOCUS       DAOOJC010000052         7848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_7852_length_7848_cov_3.423329, whole genome shotgun sequence.
ACCESSION   DAOOJC010000052 DAOOJC010000000
VERSION     DAOOJC010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7852_length_7848_cov_3.423329"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PLA01_02535"
     CDS             <1..802
                     /locus_tag="PLA01_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPD00208.1"
                     /translation="MLKGADWFSSIGTEKSKGTKVFAVGGKINNTGLVEIPMGTTLRE
                     VIYDIGGGIPGGKKFKAAQTGGPSGGCIPASHIDTPIDYDSLIQLGSMMGSGGLIVMD
                     EDTCMVDIAKFFLEFTVEESCGKCPPCRIGTKRMLEILERITEGKGEDGDIEKLEILA
                     KNIRASALCGLGQTAPNPVLSTLRYFKDEYIAHVRDKKCPAGVCKSMMQYVINADSCK
                     SCGICAKNCPVGAISGEKKMPYVIDQDKCIKCGVCMTKCPFKAIFKNA"
     gene            823..2571
                     /locus_tag="PLA01_02540"
     CDS             823..2571
                     /locus_tag="PLA01_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPD00209.1"
                     /translation="MEMVNITIDTRKIQVPAGITVLEAARQANIDIPTLCFLKDINEI
                     GACRMCVVQIKGARSLQAACVYPVSEGLEVYTQSPAVREARKVTLELLLSNHDKKCLT
                     CVRSRNCELQKLAEELNIKDIRFEGEMGKFPVDDFSPSIVRDPNKCILCRRCVSMCKN
                     VQNVAVIDTMERGFKTIVSSAFNKSLSEVPCVNCGQCITVCPVGALREKDDTDKVWQA
                     LDDPDMHVIVQTAPAVRVALGEEFGMPIGSRVTSNMVAALRRLGFTKVFDTDTAADLT
                     IMEEGTELLNRIKNGGKLPLITSCSPGWIKFCEHNYPEFLDNLSTCKSPHEMFGAVLK
                     SYYAEKNGIDPSKIFVVSIMPCTAKKFEAQRPEMSATGYPDVDVVLTTRELARMIREA
                     GIDFKDLPERTFDDPMGEASGAGVIFGATGGVMEAALRTVAEILSGKSIDNIEYNQVR
                     GVEGIKEATIELEGITLKAAVAHGLGNAKKLLDKIKAGEADYHFVEIMACPGGCVNGG
                     GQPIQPSEVRSWIDLRQERAKAIYEEDKSLPIRKSHENPKVKALYDEFLGEPGSHKAH
                     ELLHTHYTERENYPVK"
     gene            2925..3770
                     /locus_tag="PLA01_02545"
     CDS             2925..3770
                     /locus_tag="PLA01_02545"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPD00210.1"
                     /translation="MNQLFPIKSTIDSRRSARSYKMIPIDYEVIESIKNFAKSIPVPF
                     DHNVEIRFFNADPTKALYSVMKSPPNNVAFIAETDVVSISKAGFVGELLILFAQSKDI
                     STCWYGHYKLSELERLMPHLQSTVQLEESNMGYGYSKGETTGRRAICISPLGYYEDRG
                     LRLMDRITKTTISFKRKEVKELLENSNDYNKLSDDILYALDLGRKAPSAANSQMWRFA
                     FDDNFKTITISMPVGYKHFKWEHPNVDIGICACHVWLGLIDKGYNPTVTVNEDLGRAV
                     WRISI"
     gene            3787..4404
                     /locus_tag="PLA01_02550"
     CDS             3787..4404
                     /locus_tag="PLA01_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD00211.1"
                     /translation="MPLQLYDKEKIIHACFNVFAQHGYVNTSTAMLAEAAGISKALIF
                     HHFKSKKYLYLSVLDRCIEKIKNEIGFDILLENYDFFEAKEKFSVIKFNYYKSNPNIM
                     SIMTEAFYSTPDELKAEVQIKYGKLLAESDKEWRRAFEKVSLKKGVDRDKAFRLVMNT
                     LYYLDMKYFSNLKNNDDLEETRLHEFIEERSSFLSMIRFGIEQLS"
     gene            4553..7102
                     /locus_tag="PLA01_02555"
     CDS             4553..7102
                     /locus_tag="PLA01_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPD00212.1"
                     /translation="MNLYDKVSTGLEGFDRVIDHLRIGDNVVWQVDCVSSYKKMVDYF
                     VESAIAENMKLIYIRFANHEPILDAGQDIKTYHIDARKGFESFATEIHNLIKEEGKRV
                     FYVFDCLTDLLEYWNSDLMIGNFFKATCPYLYELDTVAYFAVKRNFHTYSTIAGIRET
                     TQILLDLYQINDKTYIHPLKVWQRYSPTMFFPHVIQDREAICITSSTDASELFNSINR
                     GEIRLDHWNTVFKEAKRMLSFSQKKQEAVKKRLMYMLIGSDSRMFQLCDRYFTLKDIL
                     DIASREIGTGFIGGKSVGMLLARKILEVDGGDIITPFLEPHDSFFIGSDVFYTYIVQN
                     GWWRLRTKQKTPEGYYKYAAELKEKLLHGSFPKDIQEQFVQMLEYFGQSPIIVRSSSL
                     LEDNFGNAFAGKYESVFCVNQGTPQERYEAFEQAVRTVYSSTMNEDALSYRMNRGLGL
                     QDEQMAILVQRVSGDRYGDWFFPHVAGVGNSSNLYVWDESIDMNAGMLRLVFGLGTRA
                     VDRTDGDYVKVVCLDNPFRIPPMNYEDQKKFSQHRLDLLSFTENVLESRDLDEVLSLP
                     LKADEKLFVSPDYAAEARMREMGYMNRKTPYVLDFKKLFTDTKFAAVMREMLALLSKA
                     YDYPVDIEFTANFNKDNGFKINLVQCRPLQTKGLGKTVKIPEIKDVKDCFFSSKGNFM
                     GGSVRLPIDYVIFISANSYLKLSEQGKYEVARQIGLINREMKGKNAMLVGPGRWGSTT
                     PSLGVPVHFTELCNMKVLCEYSSKKEGFMPELSYGSHFFQDIVESEIFYVAIFDGYQD
                     LIFNPDRILQEENLLTSFLPESGHLKDVIHIANTGGMEIYSDIVTQKLLCR"
     gene            complement(7254..>7848)
                     /locus_tag="PLA01_02560"
     CDS             complement(7254..>7848)
                     /locus_tag="PLA01_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00213.1"
                     /translation="SFSSVAALFATKPGIVPPFINNNICIKVNRIFDTSRQEVTHTGK
                     VTVPSLKCGAFLGCEITKAQCFVNQTNETGNARDTVQVCVELDIAYKTKAQGRITYEI
                     KPFVFTTTVPLSVPENAAVNCEIQNPTCESTQLECCSVETTVHAFIKLQSMGTDSIVI
                     PFVASCPVGLCPTNTTGCTEQDSDNCCNRDAADDSVG"
BASE COUNT         2428 a         1333 c         1816 g         2271 t
ORIGIN      
        1 catgcttaag ggagctgact ggttttcaag cattggtacc gaaaagagca agggtaccaa
       61 ggtgtttgca gtgggaggta agatcaataa caccggcttg gttgagatac cgatgggtac
      121 aaccttgaga gaggttattt acgatatagg cggaggaatt ccgggtggca aaaaattcaa
      181 agctgcacag accggcggac cgtcaggggg atgtattcct gcaagtcata tcgatacacc
      241 cattgattat gattctctga ttcaattggg atcaatgatg ggatccggcg gacttatcgt
      301 tatggacgaa gacacatgta tggttgatat tgcaaagttc ttccttgagt tcacagttga
      361 agaatcctgc ggaaaatgtc ctccgtgccg tattggaacc aagagaatgc tcgaaattct
      421 tgagaggatt accgaaggaa aaggtgaaga cggtgatatc gaaaaactgg aaatccttgc
      481 gaagaatata agagcatcgg ctttgtgcgg attgggtcag actgcaccaa acccggtact
      541 tagcacattg aggtatttca aagatgaata tattgctcat gtccgtgata agaaatgtcc
      601 ggcaggggta tgtaaatcca tgatgcagta cgtgataaat gcagattctt gtaagagctg
      661 cggaatatgt gcgaagaact gtccggtagg tgcaataagc ggagaaaaga agatgcctta
      721 tgtaatagat caagataaat gtatcaaatg tggtgtttgt atgacgaaat gtccgttcaa
      781 ggccatattt aagaatgcgt agaggaagga gagatggaaa acatggaaat ggttaatatt
      841 actatagata cccgaaagat tcaggtgccg gccggaataa ccgtgttgga ggctgcaaga
      901 caggcaaaca tagatatacc taccctttgt ttccttaaag acataaatga gattggtgca
      961 tgccgtatgt gtgttgtaca gattaagggt gcaaggagcc tgcaagcagc ttgcgtatat
     1021 cctgtatcgg aaggacttga agtatataca cagtcacctg cagtcagaga agcaagaaag
     1081 gtaactttgg aattattact gtcaaaccat gacaagaagt gtttgacctg tgtaagaagc
     1141 agaaattgtg aacttcagaa attggctgaa gagttaaata ttaaggatat acgttttgag
     1201 ggtgaaatgg gcaagttccc ggtagacgat ttttctcctt ctattgtaag agatccaaac
     1261 aagtgtattc tgtgcagacg ctgtgtgagc atgtgtaaaa atgttcagaa cgttgcggta
     1321 atagatacaa tggaaagagg atttaaaacc attgtatcgt cagcctttaa taaatcactt
     1381 agtgaggtgc cgtgcgtcaa ttgcggccag tgtatcacag tttgtcctgt aggagcactc
     1441 cgggagaagg atgataccga caaggtatgg caagcccttg atgatcccga tatgcatgtt
     1501 atagttcaga cagcacctgc tgttagggtc gcactgggtg aagaattcgg aatgccgatt
     1561 ggatcaaggg taacaagcaa catggtagca gcattgaggc gtttgggatt taccaaggtg
     1621 ttcgataccg acacagccgc tgatcttacg attatggagg aaggtaccga gcttctcaac
     1681 agaattaaaa atggtggaaa gctgccgttg attacatcct gcagccctgg atggatcaag
     1741 ttctgtgagc acaattatcc cgaattttta gataatctct caacatgcaa atcccctcat
     1801 gagatgtttg gtgctgtgct taaatcctat tatgcagaga agaacggtat tgatccttca
     1861 aagatatttg ttgtatcgat aatgccatgt actgccaaga agtttgaagc acagagacct
     1921 gaaatgtctg caaccggata tcctgatgta gacgtggttc tcaccaccag ggagcttgca
     1981 agaatgataa gggaagctgg aattgacttt aaggatttac cggagagaac ctttgacgat
     2041 ccgatgggtg aagcttccgg agccggtgtt atcttcggag caaccggtgg agttatggaa
     2101 gcagctctga ggactgttgc cgagatattg agcggaaagt ctattgacaa tattgaatac
     2161 aaccaggtta gaggtgttga gggaataaag gaagctacta tagaactgga aggcattact
     2221 ttaaaggctg ctgttgcaca cggtctgggg aatgccaaaa agcttcttga taagataaag
     2281 gcaggagaag cagattatca ctttgttgaa ataatggcat gtcccggcgg ttgcgtaaac
     2341 ggtggtggac agcctataca gccatcggag gtaagaagct ggattgactt gaggcaagag
     2401 agggcaaaag ctatttatga agaagataaa tcccttccga taagaaagtc tcatgagaat
     2461 ccgaaggtta aagcacttta tgatgagttc ttgggtgaac ccggcagtca taaggcacat
     2521 gagcttttgc atactcatta tacagagagg gaaaactacc ctgtgaaata ggcaaaataa
     2581 atatgtatta aacaagatgt gatgaggtca ggctttatgc ctgacctcat ttagtatgtg
     2641 tgtttgcatc aagggtggct gaagtttctt tattgtggtc ccaatagtaa agtacttatt
     2701 atatcatttt aaagagaaga caaaagaaca tattttagac ggtacgcgat agaagtaaag
     2761 gcatccttgc ttgaaaaaag tacctttcta ttatcaataa atacatattg accaatagtt
     2821 agtaagatgc tatagttata ttaaccgatg gtcaataata tggtttaata atgctaacaa
     2881 tgattatttt atttcatgga gaaatttttt tggagggatt aaatgtgaac cagctattcc
     2941 ctataaagag tactattgat tcgcgtcgct ctgcacgtag ttacaaaatg attccaattg
     3001 attacgaggt tattgaatcc ataaaaaact ttgcaaaatc aataccggtc ccgtttgacc
     3061 ataatgttga gatcaggttt tttaatgctg atcccacaaa agctctttat tcagtaatga
     3121 aatctccacc caataatgtt gctttcattg ctgaaactga tgtagtttca atatctaaag
     3181 ccggatttgt aggagagtta ttaatccttt ttgcacaaag caaggatatt tcaacctgtt
     3241 ggtacgggca ctataaacta tcggaacttg aacgtttaat gcctcattta caatccacag
     3301 ttcaactaga agaatcaaat atgggttacg gatactcaaa aggtgaaacc accggtagga
     3361 gagcaatatg tatctcaccc ttaggttatt atgaagatag aggacttcgc cttatggata
     3421 gaattacgaa aacaactata agttttaaac gcaaagaagt taaagagctg cttgagaatt
     3481 ctaatgatta taacaagctg tcagatgaca tattatatgc cctggacttg ggaagaaaag
     3541 caccatctgc tgcaaattct cagatgtgga gatttgcctt tgacgataat tttaagacaa
     3601 ttactatttc aatgccggtg ggatataagc atttcaaatg ggaacaccca aacgtggata
     3661 taggtatttg tgcatgtcat gtatggctag ggctgattga caaaggatat aatcctactg
     3721 taactgtgaa tgaagatttg ggtagagcgg tctggaggat ttcaatctag caaggagttg
     3781 aattcaatgc ctttacagct ttatgataaa gagaaaatta tacatgcttg ttttaatgta
     3841 tttgctcaac atggatatgt aaatacctct accgcaatgt tagcggaggc agcaggaata
     3901 tctaaggctt taatctttca tcatttcaag agcaagaaat acttgtatct aagtgttttg
     3961 gacagatgta tagaaaaaat aaaaaatgaa atcggttttg atattttgct ggaaaactac
     4021 gatttttttg aagctaaaga aaagttcagt gttatcaaat tcaattatta taaaagtaac
     4081 cctaacataa tgagtattat gacagaagct ttttattcaa cacctgatga actaaaagcc
     4141 gaggtacaga ttaaatatgg aaagctactg gcggaaagcg ataaagaatg gagacgtgca
     4201 tttgaaaagg tttctcttaa aaaaggtgta gatagggata aagcttttag attggtaatg
     4261 aatacgctgt attatcttga tatgaaatat ttttcgaatc taaagaataa tgatgattta
     4321 gaagaaacac gtttgcatga atttattgaa gagagaagca gttttctttc aatgatcaga
     4381 tttgggattg aacagttgag ttgatatctc tgatgtcgat tgcctaataa tttctcatgg
     4441 ccattatgat aaaatagata caacatgatg atgtttatta tataattaag ttaataacaa
     4501 atttaacggt ttggttaatt tagaatatct ataagcagaa atggggtgtg ctatgaattt
     4561 atacgacaaa gtcagcacgg gattagaagg tttcgatcgg gtgattgacc acttacgcat
     4621 tggagataac gtggtgtggc aagtggattg tgtttcaagc tataaaaaaa tggttgatta
     4681 ttttgtagaa agtgcaatag cggaaaacat gaaactgatt tatatccgtt ttgccaatca
     4741 tgaacccata ctggatgccg gacaggatat caagacttat catattgatg caagaaaagg
     4801 ctttgaaagc tttgccactg aaatacacaa tcttattaaa gaagagggta aaagggtatt
     4861 ctatgttttc gattgcctca ctgatttatt ggaatattgg aattcggatt tgatgatcgg
     4921 taattttttt aaggctacct gtccatattt atacgagctg gatactgttg catattttgc
     4981 cgttaagcga aatttccata cctacagtac cattgccggt attcgtgaaa caacccagat
     5041 tcttctggat ttgtatcaaa tcaatgataa aacttatatt catcccctta aggtatggca
     5101 acgctattcg cctaccatgt ttttcccgca tgtaattcag gatcgggagg ctatctgcat
     5161 tacatcaagt accgatgctt cggaactttt taacagcatt aatcgtgggg aaataagact
     5221 ggatcattgg aatactgtct ttaaggaagc gaaaagaatg ctgagctttt ctcaaaagaa
     5281 gcaggaagca gtaaagaagc gtcttatgta tatgcttatc ggcagtgatt ctcgaatgtt
     5341 tcagttgtgt gatcgatatt tcacattaaa agatattctg gacattgcat ccagagaaat
     5401 cggtacaggc tttatcggcg gcaagagcgt cggtatgctt ctggcaagaa aaatattgga
     5461 ggttgacgga ggcgacatta ttacaccgtt tttggagccc catgattcat tctttatcgg
     5521 ctccgatgtt ttttatactt atatcgttca aaacggttgg tggaggcttc gcaccaagca
     5581 aaaaacacct gagggttatt ataagtatgc agcggaactt aaagaaaagc ttttacacgg
     5641 atcttttccc aaggatattc aggaacaatt tgttcaaatg ttggagtatt tcgggcagtc
     5701 tcctattatt gtacgttcca gttctttgct ggaggacaac tttggaaatg catttgccgg
     5761 taaatatgag agtgttttct gtgtgaatca gggaacaccc caggagcgtt atgaagcctt
     5821 tgaacaggct gtacgcactg tttactcaag taccatgaat gaagatgcac taagctatcg
     5881 aatgaacaga ggactggggc tgcaggatga gcaaatggcc atattggtgc aacgcgtatc
     5941 gggagaccgg tacggagact ggtttttccc tcatgtcgcc ggtgtgggga attcttctaa
     6001 cctttatgtt tgggatgaaa gtattgatat gaatgcgggg atgctccgtc tggtattcgg
     6061 attgggtacc agggcagtgg accggactga tggagattat gtgaaggtgg tatgccttga
     6121 caacccattt cgaattcctc cgatgaatta tgaggaccag aaaaaattct cgcaacatcg
     6181 gttggatctt ctctcgttta cggaaaatgt tctggaaagt agagatttgg atgaagttct
     6241 gtccctacct ttaaaagcgg acgagaaatt gtttgtaagc cctgactacg ctgctgaagc
     6301 ccgtatgcgt gaaatgggat atatgaatcg aaagacacct tatgtgttag attttaaaaa
     6361 gctctttaca gataccaagt ttgctgccgt aatgagagaa atgctggctc tgctgtcaaa
     6421 agcatacgat tacccggttg atattgaatt tacagctaac tttaataagg ataacggctt
     6481 caaaataaat ctcgtacaat gccgtccgct gcaaacaaaa ggtttaggca agacagtaaa
     6541 gataccggag ataaaagatg tgaaggattg cttcttctca agtaaaggaa actttatggg
     6601 tggaagtgta cgactgccca ttgattatgt tatatttatc agtgcaaatt catacttaaa
     6661 actaagcgaa caggggaaat atgaggtagc caggcaaata ggcttaatca atagggaaat
     6721 gaaagggaaa aatgccatgt tggtgggccc gggcagatgg ggaagcacta caccgtccct
     6781 tggggttccg gtacacttta cggaattatg taatatgaag gttctttgtg agtattcctc
     6841 aaaaaaagaa ggatttatgc cggaactatc ctatggcagc catttcttcc aggatattgt
     6901 tgaatcggag atcttttatg tggctatttt tgacggttac caggatttaa tttttaaccc
     6961 agaccgtata ttgcaggaag agaatttgct cacttctttc ttgccggaga gcggtcacct
     7021 taaagacgtg atccatatcg cgaataccgg tggtatggag atatattccg acattgtcac
     7081 acaaaagcta ttatgcagat aaagcctgat ataatagttt gtgtcctgtt atattaagtt
     7141 aagaaacact gagcatttca gtttactcaa aaatagaacc ttagttaaat taccaaggtt
     7201 ctgtttttca tatactgcac aagctttgtc actgacattt ctatcttctg acgttatcca
     7261 acactgtcat cggcggcatc tcggttacaa caattatcgg aatcctgctc tgtgcacccg
     7321 gttgtatttg tcggacaaag ccccaccggg cagcttgcaa caaaaggtat tacaatagaa
     7381 tcggtaccca ttgattgcag tttgataaag gcatgaacgg ttgtttcgac cgagcagcat
     7441 tcaagctgag tgctttcaca agttgggttc tgtatttcac agttgacggc agcattttca
     7501 gggactgaca gaggcactgt cgttgtaaaa acaaacggct tgatttcata cgttatcctg
     7561 ccttgtgctt tggtcttgta agcaatatca agctcaacac atacttggac tgtatccctt
     7621 gcatttccgg tttcatttgt ttgatttaca aaacactgtg ctttcgtaat ctcacatccc
     7681 agaaaagcac cacattttaa tgatggaaca gttactttcc cggtatgggt aacttcttgt
     7741 ctggaagtgt caaatattct gtttaccttt atgcagatat tattattgat aaacggcgga
     7801 acaattcccg gctttgttgc aaacaatgca gccacactgc tgaacgag
//
LOCUS       DAOOJC010000053         7750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_7973_length_7750_cov_4.157635, whole genome shotgun sequence.
ACCESSION   DAOOJC010000053 DAOOJC010000000
VERSION     DAOOJC010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7973_length_7750_cov_4.157635"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="PLA01_02565"
     CDS             <1..1123
                     /locus_tag="PLA01_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD00214.1"
                     /translation="NGQLFIIGRDGNYVMNTQERNRNGKVDYSIYDLYLKEILEFGNG
                     SFIKEIDGEDYIIACSTIDEINGIDLDWTVFGLTSVDLVTKEIKDARENLFSIGILCI
                     AAAFFITIIITNIHSFDSERKFNKKHAILMERERLASLGELIGGIAYNFRTPISSIQQ
                     GLEGLRELVDEYDRAIDNRLADKEHHAIVEKMKEQVQIIKPNCSYISNVISAVKGQAV
                     KLNDSTNESFNVGELLRRVKILMDHEFKHFKCELSVELNVSEETSIKGEINNLIQVIY
                     NLIYNAIESYNGQGGKIDLLISEKDRTLEFVVRDYGCGIPEDVKKKLFKQMITTKGNE
                     GTGIGVYMSYSTIKGKFGGTMAIESKEGEGTSVYVNIPL"
     gene            complement(1200..1670)
                     /gene="ispF"
                     /locus_tag="PLA01_02570"
     CDS             complement(1200..1670)
                     /gene="ispF"
                     /locus_tag="PLA01_02570"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPD00215.1"
                     /translation="MRVGIGQDSHRFDFDDKNKELVLGGIVFKDCPPLSGNSNADVVL
                     HSITNAISGVTCVNILGPVSDELCLKKGIKDSTVYLKEALKYLKGMKIVHVSISIECL
                     VPKITPMIPQMRSSIAKLLDIPEDSVGITATTGEGLTEFGQGKGIQVFSCVTVE"
     gene            1958..3679
                     /locus_tag="PLA01_02575"
     CDS             1958..3679
                     /locus_tag="PLA01_02575"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPD00216.1"
                     /translation="MRISNMFFQTLREVPAEAEIASHQLMLRSGLMRKLASGIYSFLP
                     LGYRVFRKVEQIVREEMDRAGAQELIMSALLPAEAYQASGRWEVFGAEMFRLKDRGGR
                     EFCLGPTHEEIFTDTVKSVTRSYRSLPLTLYQIQTKYRDERRPRFGVMRSREFVMKDA
                     YSFDRDDEGLDISYKKMYDAYCRIFDRLGLDYIIVDADTGAMGGSGSQEFMVKSSIGE
                     SGIAYCEACGYAANDEKAECIVQKCCEGEKCCEELSLEKVATPDARTIDELMDFFGCS
                     AREFAKTLIYKADDKVVAAMVRGDRELNETKLMNLLGCIELEMADSETVEKVTGAAVG
                     FAGPIGLKIDVIVDSEVAEMKNFVVGANETGYHYKNVNINRDFKATVVKDIRTIKEGD
                     PCPKCGAPVKVEFGIEVGHIFKLGTKYSKALDCVYLDENGKENPMIMGCYGIGINRSM
                     AAVIEQNNDENGIIWPISVAPYHVIVIPVNTTDSDQMELAEKIYSQLIASGVEVLLDD
                     RDERPGVKFKDADLIGIPIRITVGKKAKEGIVEYKRRKEKDFTATTAEEAIEMAQKEV
                     REGCAVK"
     gene            3967..4836
                     /locus_tag="PLA01_02580"
     CDS             3967..4836
                     /locus_tag="PLA01_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPD00217.1"
                     /translation="MNRKIVFLIIMALVLTGGLVFSAQDFQQASAAETFERVNFISGV
                     VTASQLNVRQGPSTKYPTVSVLNKGQYVNVLAKIGSWYVIYEPVKGIVGAVSASYIKQ
                     QGTNGTTVPSQPTSPPAQKPTAAKPTPTPMPKPTPRPTQVPKDGTNATPIEGPKTTPA
                     AGISEDEQRTLDLINKERANAGLAPLKFDMELQKVARLKAKDMVEKNYFSHQSPTYGS
                     PFDMMRQYNISFRSAGENIAGNRTVEGAVKAWMESSGHRANILNANFNYTGIGVVESP
                     RYGKIFVQMFIGR"
     gene            5063..6097
                     /locus_tag="PLA01_02585"
     CDS             5063..6097
                     /locus_tag="PLA01_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00218.1"
                     /translation="MKRIQKVLLMVLCGFIAFGIVSCKPQDGSDTSAIDIPSKTSSNN
                     TPAKTPDKTIATGQDGKPTPEPSWIIDINDHCEGMMTKEFHEMFEFILKKSSLKIINN
                     EDNSSYDCPFPRKLIKKLENSENMGKEFIKITNLYPLDDIDEQDISETVYTVGIRFGD
                     VEYLDDSIETKYVDVAKIKLKFERVDDNKSVSDIEVISKYDEDIEKYPEDYKLKETYF
                     EDVILFHTIYDLGEEAEEYKGEISCGFVYKDMKVYVSNYRITQIEVNTRGHKTPRGLE
                     VGDSKEKAKELYGLPDIGFYEDDEWKYLFYRDWSEDDSLNVLSDDYFQIAFEDNKVKS
                     MVLSAYIPID"
     gene            6331..6471
                     /locus_tag="PLA01_02590"
     CDS             6331..6471
                     /locus_tag="PLA01_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00219.1"
                     /translation="MLTDNGYNPDATSTPEKEKEYYEILKRDGMKKTEKTINLLEKYG
                     VL"
     gene            complement(6584..>7750)
                     /locus_tag="PLA01_02595"
     CDS             complement(6584..>7750)
                     /locus_tag="PLA01_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HPD00220.1"
                     /translation="ARMFKHAFISGILSVGVEVFNMSSLLTPLARHAINFLSVEGGIH
                     IKLSEGNPNKLKVDFMDSKGASISRVTERKIENSFSREDFKRCSGDEISRLNNITDFK
                     NYYVRSILNDINVDSIKNNPPKICIVSPSDFVISIVVPMLTDLGCEVASFSSTSINET
                     ESIVEEIKSKDASFAAFIDSNAETLILIDKNGNVVKDDLFLSLTSLITFKSNPNSKVV
                     VPITAPSIIEAMAKKYNGKVVRTKTSPQAVMEQMLNHNLFKNRENMDQFLLNFDAIAG
                     LVKIIEYLCVMDTSLTQTLEEIPNFYVSKKKIFCPWETKGRVMRTLITEKSGNKVELL
                     DGVKFIFENGWSLVLPDADMPLCRVYSEGISPDIAETISNKYVDKIKAIINSKG"
BASE COUNT         2522 a         1233 c         1782 g         2213 t
ORIGIN      
        1 taacggtcaa ttgtttatta tcggcaggga cggaaattat gtaatgaata cacaggaaag
       61 aaaccggaac ggtaaagttg attattccat atatgatctg tacttaaagg aaatactgga
      121 atttggtaac gggtctttta ttaaagaaat tgatggggag gattatatta ttgcatgcag
      181 tacaatagat gaaataaatg gaatagatct tgactggaca gtgttcgggc ttacatccgt
      241 cgatctggtt acaaaagaga taaaggacgc aagggaaaat ttgttttcta ttgggatatt
      301 gtgtattgcc gcagcatttt ttattacaat aataataacc aatatacaca gctttgattc
      361 ggaaagaaaa tttaataaaa agcatgccat tttaatggaa agagagaggc ttgcatcctt
      421 aggggaatta ataggaggaa tagcatacaa ttttagaaca cctatcagct caatacagca
      481 agggctggag ggcttgagag aacttgtgga tgaatatgac agagctatcg ataacaggtt
      541 ggctgataaa gagcaccatg caattgttga aaaaatgaag gagcaagtcc aaataataaa
      601 gccgaattgt tcctacatat cgaatgttat atccgcggta aaggggcagg cagtgaaatt
      661 aaacgattcg acaaacgaaa gctttaatgt cggtgagctt ttacgaaggg taaaaattct
      721 aatggaccat gaatttaagc attttaaatg cgagctgagt gtggagctga atgtcagtga
      781 ggaaacatcc atcaaagggg aaataaacaa cctgatacag gtaatttata atttgattta
      841 caatgccata gaatcataca atggtcaagg cggaaagatt gatttattaa ttagcgaaaa
      901 ggacagaaca ctggagtttg ttgttagaga ttacggctgt gggatacctg aagacgttaa
      961 gaagaagctt tttaagcaaa tgataacaac caaaggaaat gaaggaacag gaataggggt
     1021 ttatatgtcc tattcaacaa taaaagggaa attcggaggc acaatggcca ttgaaagcaa
     1081 agaaggagaa ggaaccagcg tttatgtcaa tataccctta taggaggggc attacttaag
     1141 ttttgtttct tccatactct tttcacataa ataacatgta cttttttata tttggatgtt
     1201 cactccacgg tgacgcagct aaacacctgt attcctttgc cctgaccgaa ttcggtgagc
     1261 ccttcgccgg tagttgcggt tattccgacc gaatcctccg gtatatcaag aagctttgct
     1321 atactgcttc tcatttgggg aatcatggga gtgattttcg gtacaaggca ttctattgag
     1381 atggatacat ggactatttt catgcccttt aaatatttaa gggcttcttt gaggtagacc
     1441 gtgctgtcct taatgccttt cttgaggcac aactcatcgc ttaccggtcc aagtatgttg
     1501 acacaggtga ccccagagat ggcatttgta attgaatgaa gaacaacgtc tgcgttgctg
     1561 ttgccggata gaggtggaca gtctttaaat acaatacctc ccagcaccag ttctttattt
     1621 ttatcatcaa aatcaaacct gtggctgtct tgcccaattc caaccctcat tacaacccat
     1681 ccttttttgt agtcgattcg ttaacaatta tataccagaa gacactcata agcaacagaa
     1741 actgaaactg cgggtgtaca gtatttaagt gacgaaatat atggaagaaa caatgtgaag
     1801 caagttgtag ccgcctgtta aatttaatgc accctgaaac tggtacagtc ttgacatagt
     1861 atggcagtta tattaatatt gaactataaa attttgtgat gaatattcgt cccataagag
     1921 tggatggggc ttttttatat tttaaggagg tttagttatg agaatttcca acatgttctt
     1981 tcagactctt agagaagtac cggcagaagc tgagatagcc agccatcagc ttatgcttag
     2041 atcagggctt atgaggaagt tggcttcagg tatttattct tttttgcctt taggctacag
     2101 ggtgtttaga aaagttgaac agattgttag agaggaaatg gatagagcgg gagctcagga
     2161 attgataatg tctgcacttt taccggcaga ggcgtatcag gcgtcgggcc gttgggaggt
     2221 attcggtgcc gaaatgttca ggctcaagga caggggcggc agggaatttt gcttagggcc
     2281 aacccatgag gaaatattta ccgatactgt gaaaagtgtt acaaggtcct acaggtcact
     2341 tccccttact ctatatcaaa ttcagacaaa gtacagggat gagagaagac cgagatttgg
     2401 tgtaatgagg tcgagggagt ttgtgatgaa agatgcatac agctttgaca gggatgatga
     2461 gggactggat atatcctata aaaagatgta tgatgcttat tgcagaatat ttgaccgttt
     2521 aggtctggat tatataatag ttgatgcaga caccggggca atgggtggtt ccggatccca
     2581 ggagtttatg gtgaaatcat ccattggaga atcgggtatt gcatactgtg aagcctgtgg
     2641 atatgctgcc aatgatgaga aggcagagtg tatcgttcag aaatgctgtg aaggtgaaaa
     2701 atgctgtgag gagttgagtt tggaaaaagt tgctactcct gatgccagaa ccattgatga
     2761 gcttatggac tttttcggtt gttctgccag agagtttgcc aagacattga tttacaaagc
     2821 agatgacaaa gtggttgctg ctatggtgag gggcgacagg gaactcaatg agacaaaact
     2881 tatgaatctt ttaggctgta tagaacttga gatggccgac agcgaaacag tggaaaaggt
     2941 taccggtgct gcggtaggat ttgccggacc gataggcctt aaaattgatg ttatagtgga
     3001 ttccgaagtt gcagagatga aaaattttgt tgtgggagca aacgagacag gctatcacta
     3061 caagaatgtc aatataaaca gggattttaa agccactgtg gtaaaagaca tcagaaccat
     3121 taaggaggga gacccttgtc caaagtgtgg tgctcctgtg aaggttgaat tcggaataga
     3181 ggttggacat atattcaagc tgggaaccaa gtattcaaaa gctttggatt gtgtgtatct
     3241 tgatgaaaac gggaaggaaa acccaatgat tatgggatgc tacggaatcg gaataaacag
     3301 aagcatggca gccgtaattg aacagaataa tgatgaaaac ggaataatct ggcctatatc
     3361 agtggcaccg tatcacgtta ttgtaatacc tgtgaatacc accgatagtg atcagatgga
     3421 gttggcagaa aaaatatatt cccagcttat agcaagtgga gtggaagtat tgctggatga
     3481 cagggatgaa aggcccggag tcaaatttaa ggatgctgac ctcattggga ttcctatcag
     3541 aataacggta gggaaaaaag caaaggaagg cattgtggaa tataagagaa gaaaagaaaa
     3601 agattttact gcaactacag cagaggaagc aattgagatg gcacagaaag aagtcaggga
     3661 aggctgtgca gtaaaataaa gaaaatataa tactgcttat gatgatatag tgcaatattt
     3721 ttattgacag tattttttgg aagtgaattg accgaaaagc tcaaggactt gatgcccttg
     3781 ggctttttta gtgggatttc atgaagtgaa atactacaag tttgttatat taaaagtttt
     3841 ataattttga gtttcttatg taaataattc atctaaattt gtcaatacta tattcatcaa
     3901 cgtaattacc atttaaataa aatttatctt gtttattttt taatttgata agttggaggg
     3961 attttcatga acaggaagat agttttttta atcattatgg ctcttgttct gacaggagga
     4021 ctggtttttt cagcacagga ttttcagcag gcttctgcgg ctgaaacctt tgagagagtc
     4081 aactttataa gcggtgtagt gactgccagt caactaaatg tcagacaggg gccgtctacc
     4141 aagtatccta cggtgtcagt acttaataaa gggcaatatg tgaatgtatt ggcgaaaata
     4201 ggcagttggt atgtgattta tgaaccggtt aaaggaatcg ttggagcagt atctgcaagt
     4261 tatatcaagc aacaaggtac aaatggaaca acggttccaa gtcagcccac aagtccgcct
     4321 gcacaaaaac cgacggctgc aaaaccgaca cctacgccaa tgcccaagcc aacaccaagg
     4381 cccactcagg tgcctaaaga cggtaccaat gcaactccaa tcgagggacc aaagactaca
     4441 ccagctgccg ggataagtga agacgaacag cggacattgg accttatcaa taaggaaagg
     4501 gccaatgcag gacttgcacc cctgaagttt gatatggaac tgcaaaaggt cgcaagatta
     4561 aaagccaagg atatggtgga aaagaattat ttctcacatc aatcaccgac gtacggttca
     4621 ccctttgata tgatgagaca gtataacata agcttcagaa gtgccggaga gaatattgca
     4681 ggaaaccgga cggttgaagg agcagttaaa gcatggatgg aatcatccgg acacagggct
     4741 aatatcctta atgccaattt taattatacc gggataggag ttgtggaaag cccacgatac
     4801 ggaaaaatat ttgtgcaaat gtttatagga agataaagga acagagggac gggtatcttg
     4861 tccctctttg ctttctaccg gaaactatcg taattgtaaa actaatataa tatagtataa
     4921 ttaatatagt gcataccaga ttgattggaa caatttgtac ggtaagaggc gaatgccaga
     4981 tatttctgtg gatttggagg cattataaag atgtgggata aattgacgga aatttatccg
     5041 ttattttttt aaggaggtaa aaatgaaaag aattcaaaaa gttttattaa tggtgctgtg
     5101 cggttttatt gcattcggta ttgtgagctg taagcctcaa gatggtagtg acacgtctgc
     5161 aatcgatata ccttcaaaaa cttcttctaa taatacgcct gccaaaacac ccgataaaac
     5221 aatagctacc ggtcaagacg gtaagcctac gccggaacca tcatggataa tcgatatcaa
     5281 tgaccattgc gaagggatga tgactaaaga atttcatgaa atgtttgaat tcatactcaa
     5341 gaagagtagt ttgaaaataa taaacaacga agataattca tcttatgatt gtccttttcc
     5401 gcgaaagcta ataaagaaat tggagaattc tgaaaacatg ggcaaagaat ttattaaaat
     5461 tactaacctg tatcccctgg atgatattga tgagcaagat atatccgaaa ctgtttatac
     5521 tgttggaata agatttggtg atgtagagta tttagatgat tctatagaaa caaagtatgt
     5581 agatgttgca aaaattaaat tgaaatttga aagggttgat gataataaaa gtgttagcga
     5641 catagaagtt atttccaaat acgacgaaga tattgagaaa tacccggaag attataaatt
     5701 aaaagagact tattttgaag atgtaatact atttcataca atatatgact tgggagaaga
     5761 agccgaagag tataagggtg aaatatcgtg tggatttgtt tataaagata tgaaagtgta
     5821 tgtatctaat tataggatta ctcagattga agttaataca aggggtcata agacaccgcg
     5881 gggattggaa gtaggtgata gcaaagagaa ggctaaagag ttatatggtt tgccggatat
     5941 cggtttttat gaagatgatg aatggaaata tctattttat agagattggt ccgaggatga
     6001 tagtttaaat gttttgtctg atgactattt tcaaattgct tttgaggata acaaggtaaa
     6061 aagtatggtt ttgtcagcat atattcctat agactgatta aggtgttgcc tttgattaga
     6121 tatattggat cgtagcaaac cagatatgag gttttgtgcg tgatatggaa atcggagtac
     6181 atattaaaaa gatgtagaga ggtgctataa atgtataaga tgacaagtgt atatttaaaa
     6241 tgattgcaat atttattgcc ctgctattga catgcggatt ccaaaaagaa aacaatcctg
     6301 gggtcaatac tacagctata catactgacg atgttaacgg ataatggtta taatcctgat
     6361 gcaacaagta caccggaaaa agaaaaggaa tattatgaaa tattaaagag agacggcatg
     6421 aagaaaacag aaaaaacaat taatttgctg gaaaagtatg gcgtgttatg atatcaaggt
     6481 tggcggttac ttaataaatt cacatggaaa gtttaatgac agaagaaaaa aggaatgcat
     6541 aagaaatcta agagtacaaa ggggctttag cccctttact ccattaaccc ttgctattga
     6601 taattgcctt tattttgtct acgtatttat tggagatcgt ttctgctatg tcgggggata
     6661 ttccttccga atatactctg caaaggggca tatccgcatc cggcagtacc agagaccagc
     6721 cgttttcaaa aataaacttc acaccgtcta aaagctccac cttgttgccg cttttttcag
     6781 ttatcagggt tctcattacc cggccctttg tttcccaagg acagaatatt ttctttttac
     6841 ttacatagaa attgggtatt tcttcaagag tttgcgtgag ggaggtatcc ataacacata
     6901 agtactcaat tatctttacc aaacctgcga tggcatcgaa atttagtaga aactgatcca
     6961 tattttcgcg gttcttaaaa aggttatggt ttagcatttg ttccattacc gcctgaggag
     7021 aggtttttgt ccttaccacc ttgccattgt atttttttgc cattgcttca ataatcgacg
     7081 gtgctgttat gggcaccact actttggaat tcgggttaga cttaaaggtt ataagggaag
     7141 taagagataa gaaaaggtcg tctttaacta catttccgtt cttatctata agaataaggg
     7201 tttcggcgtt gctgtctata aaggcggcaa agctggcatc tttgctctta atctcttcaa
     7261 cgatggactc agtctcattt atactggtgg aggaaaagct tgcaacctca caacctaagt
     7321 cggtaagcat gggtacaacg atggaaatta cgaaatccga cggtgaaact atacaaattt
     7381 ttggaggatt gttctttatg gagtcaacat taatatcatt taatattgaa cgcacatagt
     7441 agtttttgaa atccgtaata ttgttgagtc ggctgatttc gtcaccggag caacgtttaa
     7501 aatcttccct tgaaaaggaa ttctctattt ttctttctgt aaccctgctg atgcttgcac
     7561 cctttgaatc cataaagtca accttgagtt tgttcggatt gccttcgctg agcttaatat
     7621 ggattccgcc ctcgacagag agaaaattga tggcatgacg ggctaatggg gttagaagac
     7681 tgctcatgtt aaatacctcc acgcccacgg agagaatgcc ggaaataaag gcgtgtttaa
     7741 acattctggc
//
LOCUS       DAOOJC010000054         7725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_8001_length_7725_cov_3.699087, whole genome shotgun sequence.
ACCESSION   DAOOJC010000054 DAOOJC010000000
VERSION     DAOOJC010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7725
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8001_length_7725_cov_3.699087"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1072)
                     /locus_tag="PLA01_02600"
     CDS             complement(95..1072)
                     /locus_tag="PLA01_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00221.1"
                     /translation="MIKRLRIISLFLFIIGTLLIFSASYFELFFPPKNAFYLGIFGDI
                     ILAIIRSVGVSLITSTFINLLKFWVESTETLVKHDFINRLSRNEMKDLKEKLEKELYF
                     RYNNHDKDNFYNFFEQELSSLLNECYYQSYKTRIECQVTDNYISKTIRKKFEIINPSK
                     KECTIKIPFVAEMQRVEQVENNKLFQIKKFVVDSEDFTANINEGLQISDDCPHISDAY
                     CVKIHTSWDVKVKKQCTIDMVIETIVPLNDVCFSNKITMPCKNYNVIFILNDPSYQLS
                     WHSFGFMGNHKDRIVEEPMDNGLEIGFKDWILPGDGVVISITKCEKNHV"
     gene            1417..2730
                     /gene="lysA"
                     /locus_tag="PLA01_02605"
     CDS             1417..2730
                     /gene="lysA"
                     /locus_tag="PLA01_02605"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPD00222.1"
                     /translation="MFVSKALKINSKNHLEIGGCDCVDLVNEYGTPLYVMDEGLIREN
                     CGLYKNAMDKYYNGNGLVLYASKALCTMAMCRIAQQEGLGLDVVSGGELYTAIKAGFP
                     MDRIYFHGNNKTMEELELAIDSNIRRIVVDNRQELHRINEIASSKGKTASISFRIKPG
                     IDAHTHDFIQTGQIDSKFGVALENGEALEIVSEAVKLGNINVAGLHCHIGSQIFDIKP
                     FEEAARVMLEFIAQIKEQLGVEIEELNLGGGYGIKYTPQDDPIEYDKYIESVSKVIRN
                     VCDNKGIKLPFIVMEPGRSIVASAGITLYKVGTIKDIKGVRKYVAVDGGMGDNPRFAL
                     YQSKYDAVIANRPDAPKTEKITIAGKCCESGDLLAKDIMMPEVKEGDILAVLATGAYN
                     YSMSSNYNRIPRPPVVLVKDGKARVIVKREDYNDIIRNDVLPEDL"
     gene            2847..4079
                     /locus_tag="PLA01_02610"
     CDS             2847..4079
                     /locus_tag="PLA01_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPD00223.1"
                     /translation="MKIKVKETFFVLVLIAVVFCNTVYSNASENMLEYRTIDDIVECY
                     QRNPFRINVSVSDPYEIEPNAEPPYVAGSLKREYLEEALNCVNFLRYLVGLPDDIELN
                     DTYNNYAQHATIVFERLKQLTHYPKKPDDMPDDFYSLAYNGASNSNIAYGYASLMNSI
                     MGYMKDEDDSNISIVGHRRWLLNPSMTKIGFGQCGIFYSAYVADMSRSPSIKFDFISW
                     PARNNMPREYFYNSNIPWSVNLGSNYANPSLDDVEVTLTRRSDNKVWIFNKNNIEEYG
                     YFNVNNDGYGMKKCIIFRPNGVGSYYKNDVFDVNIKGIRHYTDGPTEINYTVEFFSLT
                     DAIDEREKNFKYGDVNGDGDIDSLDLTILKRYLLRKMDISDDRPADLDGDGDINSTDL
                     TLLKRYILRKIDRFPVET"
     gene            4276..6039
                     /locus_tag="PLA01_02615"
     CDS             4276..6039
                     /locus_tag="PLA01_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPD00224.1"
                     /translation="MDIKKITTYAIVYALIVISVPVFFNSLWLKPMPDLEIYDGSLDF
                     LGSNAMADIYELVKEYPERVVGSENAKKSARWIGDRFKKLGLDTYTQEFTCWSFKESD
                     EEPSLSDYYEKVKGINVVGVSKGKSEEILLIGAHRDVLYTIEGAQDNATGTASMLELA
                     RVLTDEEHYYTYMFLSFDAEEIGLVGSEKFVKSNPGLNIKLALILDCVGYKNADTIGY
                     YQFATGKGASPLWTVSLAQKLMNERNLPMYYLDEDGGFDTLSINTFNVYLDKLLKKRV
                     FGSSNTDTGPFVDRSIPAIGFIAAKTGEIVDPELAFHTENDNTSLVSEFTLDRIGKFA
                     EQYIRSVELNRFEGDFNKSFYVVAGEKYLVFLPIAVFMVFIAVLFIILWALTASDVIK
                     NSKPFLGFLKKEIKWLVPILLISMVSTFSLMLLKEVMVLDFVAILFLLALFIIGFLTI
                     LSVLVLRFVFVIKSKADYYETTKYQRILLNTVYIIVFFLVTIIYNVLVAAVLLGLPII
                     IMGRMGFKNIGIRILWGVIFAIWSVIEAELLLICLQPYILEILTFKPVFLIFLFVFIW
                     CTTFIYTVSSPAIPRRNLYVR"
     gene            6134..>7725
                     /locus_tag="PLA01_02620"
     CDS             6134..>7725
                     /locus_tag="PLA01_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPD00225.1"
                     /translation="MEIIVGHMNTDYDSISSMVAASCLYPEAVMVLPGTVTKYVRKFL
                     NLYRDSFDFKTLNDIDINQVNRIIIVDTNSKSRLDSKLSDILDRPGVDKIVYDHHPEA
                     DDMIETKNLKLEKTGACVTMLVREIQNLNIDISPLEATVMILGIYADTNCLTFESTTA
                     EDAHTVGFLLSKGANLQVVEEYLSTHLSTEQMELLALMSKNAKVIDVKGFQIAVSTVK
                     LDKYVDNAAFLTGKLMEERDVDAFFSILRMDDKTYIIGRSDEEEIDVGFILGFLGGGG
                     HPGAGSAKTSEEDIEMLSSRLEDLLNNNVLSVTRAKDIMSTPVKTVTEDTSIDEIGDI
                     ILRYGHSGFPVIKEGELCGIISRRDVEKAIYHGFGHSPVKAYMTKKVITIDPETPVRT
                     IENLLVEHNIGRLPVISGNELVGIVTRTDIIATLFGNNSKYWSKKNKASDLSRCSYKL
                     NNLADRIKDLPERVVRALADAGRIADEEGFVAYVVGGFVRDLILGIEHVHVVLARDLL
                     VVQAIPHVGVDEVDALDALDVVG"
BASE COUNT         2515 a         1245 c         1597 g         2368 t
ORIGIN      
        1 acaactccct tcattaccag tatatttcct ctcaaatcat tgagtgtttg tcttaagtta
       61 ttttatacat tattctgaca caaaactttg aattttaaac atggtttttt tcacatttgg
      121 tgattgaaat aacaactccg tcaccgggaa gaatccaatc cttgaaaccg atttcaagac
      181 cattgtccat tggctcttcc acgattctgt ccttatgatt tcccataaat ccaaaactat
      241 gccatgataa ttggtaagaa gggtcgttta atataaaaat tacgttgtaa ttcttgcagg
      301 gcatggttat tttgtttgaa aagcatacgt catttaaagg aacgattgtc tcaattacca
      361 tatctatcgt acactgcttt ttcactttca catcccaaga tgtgtgaatc ttgacacaat
      421 aagcatcgct aatgtgagga cagtcgtccg agatttgaag tccttcatta atatttgctg
      481 tgaaatcctc agaatccaca acgaatttct tgatctgaaa cagtttattg ttttctacct
      541 gttcaacccg ttgcatttct gctacaaagg gtatttttat cgtacattct ttttttgaag
      601 gatttataat ttcaaacttt ttccttatag tttttgatat atagttatcg gtaacctgac
      661 actcaatcct tgttttgtag ctctgatagt aacactcatt caatagggaa gacagctctt
      721 gctcaaagaa gttgtaaaag ttgtccttat cgtgattgtt atatctgaaa taaagctctt
      781 tttctaattt ctcctttaga tccttcatct cattcctgct cagtctgttg atgaagtcat
      841 gcttaaccaa tgtttctgtc gactccaccc agaatttcag caggtttata aacgtggagg
      901 taatgaggga aactccaacg cttcttatta tagccaaaat gatatcaccg aagataccca
      961 gataaaatgc gtttttaggc ggaaaaaaca gctcaaaata tgaagccgag aatattaata
     1021 acgtcccgat tataaataaa aacaaagaaa taatacgaag ccttttgatc ataatatatc
     1081 atctccatac atttcgtatt agtataatat attatgtata atagaaaata ataacaggtt
     1141 ttcgttgtga ttataatttt tattaataaa attattgggt tgaatttaag aaattaccga
     1201 tttttcataa ctttaataat atacacccat caaaagctta aaaaaacaat tgcaccaata
     1261 tgcaggattg aatattgact cttgcaatta ttaatgttac cataatgtat catatgatat
     1321 atcaatattg tcgaaaagta tcaaatgcaa tgataagcat atgtatgatt cgattatgtt
     1381 tatgcattaa aatattatgt ggaaggtgga aagaaaatgt ttgtgtcgaa agcactgaaa
     1441 atcaacagta agaatcatct ggaaatcggt ggatgcgact gtgtggactt ggtaaatgaa
     1501 tacggtactc ccttgtatgt tatggatgag ggtctgatta gggaaaactg cggtttatat
     1561 aaaaatgcca tggacaaata ttataacgga aacggtttgg tcctttatgc aagcaaggca
     1621 ttgtgtacta tggcaatgtg cagaatagca caacaggagg gtctgggtct ggatgtggta
     1681 tccggcggag aactgtatac tgcaataaaa gcgggatttc ccatggacag gatatatttt
     1741 cacggcaaca acaagacgat ggaagagttg gagctggcta tcgacagcaa tattagaaga
     1801 atagttgtcg ataacagaca ggagcttcac cgcataaatg aaattgcatc cagtaaagga
     1861 aaaaccgcca gtatttcctt tagaataaag ccgggaattg atgcccatac ccatgacttc
     1921 atacaaaccg gtcagattga ttcgaaattc ggtgttgccc ttgaaaacgg tgaagctctg
     1981 gagattgtgt cagaggcagt aaagctaggt aatattaacg tagcaggatt gcactgccat
     2041 atcggatccc agattttcga cataaaacca tttgaggaag cagcaagagt gatgctggag
     2101 tttatcgccc aaataaagga acagttgggg gttgaaattg aagaattaaa tcttggagga
     2161 gggtatggta taaaatatac tccccaggat gatcccatag aatatgacaa atacattgag
     2221 tctgtctcca aggttataag aaatgtttgt gataataaag gtattaagtt acccttcata
     2281 gttatggagc ccggaaggtc cattgttgca tctgcaggta tcacattgta caaagtcgga
     2341 acaataaaag atatcaaggg tgtgagaaaa tacgtagcgg ttgacggcgg tatgggggac
     2401 aatccgaggt tcgccctgta tcagtcgaag tatgatgccg ttatcgcaaa cagacccgat
     2461 gcaccgaaaa ctgagaaaat aacaattgcc ggcaagtgct gcgaatccgg cgaccttttg
     2521 gcaaaggata ttatgatgcc ggaagtaaag gaaggagaca ttttagctgt tctggctaca
     2581 ggagcttaca actattcaat gtcaagcaat tacaaccgta tacctaggcc tcctgttgtt
     2641 cttgtaaagg acggtaaggc aagggttatt gtaaaaaggg aggactataa tgatatcata
     2701 agaaatgacg ttttgcccga agacttataa actaaataat atcttcattt actaaaaaaa
     2761 gtaacagaaa cagaaagaaa atgttgagta aatttcaatg atatgctatt aattatatat
     2821 atataacaaa caaaggggga gtagatatga agattaaagt aaaagaaaca ttttttgttt
     2881 tagttttaat tgcagtggtt ttctgtaata cggtttattc taatgcttca gagaacatgt
     2941 tggagtatag gacaatcgat gacatagtgg aatgctacca aagaaatcca ttccgtatta
     3001 acgtatctgt ttctgatcca tatgagattg aaccaaatgc tgaacctcca tatgttgcag
     3061 ggagtttaaa aagagagtat ttggaggaag cattaaattg tgttaatttt ttgcgttatc
     3121 tggttgggct tcccgatgat attgaattaa atgacaccta taacaactat gcccagcatg
     3181 ctacaatagt atttgaaaga ttaaaacaat taactcatta tcctaaaaaa cccgatgata
     3241 tgcctgatga tttttatagt cttgcatata atggagcttc aaattctaat attgcttacg
     3301 gctatgcatc tttaatgaat agtattatgg gatatatgaa agatgaagat gatagtaata
     3361 ttagtatcgt aggacaccgc agatggctgt tgaatccaag tatgacgaaa ataggatttg
     3421 gacagtgtgg tattttttat agtgcatatg tagctgatat gagtagaagt ccctccatta
     3481 aatttgactt tatatcatgg cctgcaagaa ataatatgcc tagggagtat ttttacaata
     3541 gcaacatacc ttggtcagtt aatcttggca gcaattatgc taatccatct cttgatgatg
     3601 ttgaagttac tcttacgaga agaagtgaca acaaagtatg gatttttaat aaaaacaata
     3661 ttgaagaata tgggtatttt aatgtaaata atgatggtta tggaatgaaa aagtgtatta
     3721 ttttcagacc caatggtgtc ggtagttact ataagaatga tgtttttgat gtaaacatca
     3781 aaggaataag gcattatacc gacggaccaa ctgagattaa ttacacagtt gaatttttca
     3841 gtttgacaga tgctattgat gaaagggaaa aaaattttaa atatggagat gtaaacggag
     3901 atggagatat agattcattg gatttaacga tattgaaaag atatcttcta aggaaaatgg
     3961 atatatcaga tgatagaccg gcagatttag atggtgacgg agatataaat tcaactgatc
     4021 tcacattgct taaacggtat attttaagaa aaatcgatcg ttttcctgtt gaaacctaaa
     4081 tttttaatgt aaggtttttc ttcttcagat tataaaggtt ggcagctatc taaaaaagat
     4141 agatgtcaac ctttatttat tgttaagcat ggtgcaaata tgtattttgt caaagcaaat
     4201 agcaaaaaaa aagtgctctt ttttttgttt atatctgata taattaattt tataaaaaaa
     4261 attgaggggg agaaaatgga tataaaaaaa ataactactt acgctatagt ttatgcttta
     4321 attgttatat ctgttcctgt tttctttaac agcttatggt taaaaccaat gccggattta
     4381 gaaatctatg atggatccct tgatttttta ggctcaaatg ctatggcgga tatttatgag
     4441 cttgtcaagg aatatcccga gagagtagta ggcagtgaaa atgcgaaaaa atctgcacgg
     4501 tggattgggg atagatttaa aaaattgggg cttgatacat atacgcaaga atttacctgc
     4561 tggagtttca aagaaagtga tgaagaaccc tcattaagcg attattacga aaaagtcaaa
     4621 gggataaatg ttgttggcgt aagcaagggt aagagtgaag aaatacttct tatcggtgcc
     4681 catagggacg tactttatac cattgaaggt gcccaggata atgcaacagg aacggcatcc
     4741 atgctggagc ttgcccgggt tttgaccgac gaggagcact actatacata tatgtttcta
     4801 tcctttgatg ctgaggaaat aggtcttgta ggttcagaga aattcgttaa aagcaacccg
     4861 ggtcttaata tcaagctggc tttaattctt gactgtgttg gatacaagaa tgcagatacc
     4921 attggttatt accagtttgc caccgggaaa ggtgcttcac ctctttggac tgtttcatta
     4981 gctcaaaaac tcatgaatga aagaaacctg ccaatgtatt atcttgacga ggatggcggg
     5041 tttgatactt tatcaataaa tacattcaat gtttatttag acaaattact taaaaaaaga
     5101 gtgttcggca gttcaaatac cgatacggga ccttttgtcg acagaagtat tcctgcaata
     5161 ggttttattg ctgcaaaaac cggagaaatt gtggatcccg agttggcttt tcacacagaa
     5221 aatgataaca cttccttggt aagtgaattt acccttgaca ggattggaaa atttgcagag
     5281 cagtatataa gaagtgttga actgaaccga tttgaaggag attttaataa aagcttttac
     5341 gtggtggctg gagaaaaata cttggtcttt ttacccatag ccgttttcat ggtttttata
     5401 gctgttttgt ttattatatt gtgggcattg acggcatctg atgtcattaa aaattccaaa
     5461 ccttttttag ggtttttgaa aaaagagatc aaatggctcg tcccgattct tttgatatca
     5521 atggtatcaa ccttttccct gatgctgtta aaagaagtca tggttttaga ttttgttgct
     5581 atattattcc tccttgcact ttttattata ggttttctca caatattgtc agtacttgtt
     5641 ctgagatttg tatttgttat taagagtaag gcagattact atgagacaac aaaatatcag
     5701 agaatattac ttaatactgt ttatatcatt gtcttctttc tggtaacaat aatctataat
     5761 gtcctggtgg cagctgtgct tttgggactt cccataatta taatgggcag gatgggattt
     5821 aaaaacatag gtatcagaat cctttgggga gttatatttg ccatatggtc agtaattgag
     5881 gcagagttgc tgctaatttg ccttcagcca tatattttag aaatactaac ctttaagccg
     5941 gtgtttttaa tttttttgtt tgtatttatt tggtgcacaa cctttatcta taccgtaagc
     6001 tcaccggcta taccgagaag aaacttgtac gtccggtaaa tatgaagaaa aatataccgt
     6061 aaaatctaaa taatacttta gtaaaaacta cttcggcaga tgacggttgt atttagttta
     6121 aggagtgata atagtggaaa taatagtagg acatatgaat acggattatg acagtatttc
     6181 atccatggta gctgcgagct gtctttatcc tgaagcagtt atggttcttc ctggaacggt
     6241 gacaaagtac gtcaggaaat ttttgaacct atacagggat tcctttgatt ttaaaaccct
     6301 caacgatata gacataaatc aggttaacag aataattatt gtggacacca actcaaagtc
     6361 cagattggat agcaagcttt cagatattct cgatagaccc ggtgttgata aaatagtata
     6421 cgaccaccat cctgaagctg acgatatgat tgaaacaaaa aacttgaaat tagaaaaaac
     6481 cggtgcatgt gttacaatgc ttgtgaggga aatacaaaac cttaatattg acatttcacc
     6541 cctggaagca acggtgatga ttcttggaat atatgcggat accaattgcc tgacctttga
     6601 aagtacaacc gctgaagatg cccataccgt tggatttctg ctgtctaagg gagcaaacct
     6661 tcaagtggta gaagaatacc tctcaaccca tttgagcacg gaacagatgg aacttcttgc
     6721 ccttatgagt aaaaacgcca aagttatcga tgtaaaaggc tttcaaattg ccgtctcaac
     6781 ggtaaagctt gataagtacg tggacaatgc agccttttta accggcaagc tcatggaaga
     6841 aagagacgtt gatgcttttt tctcaatact gagaatggat gacaaaactt atatcatagg
     6901 aagaagcgat gaagaagaaa tagatgtagg ttttatattg ggctttttgg gaggaggagg
     6961 tcacccggga gccggttcgg ccaaaacttc cgaagaggat attgaaatgc tttcatcccg
     7021 gcttgaggat ttactcaata ataatgtttt atctgttacc agggcaaaag acataatgag
     7081 cacaccggta aaaacggtaa ctgaggatac cagtattgac gaaatcggag atattatact
     7141 gcggtacggt cattcgggat ttccggttat aaaagagggc gagctttgcg gaattatatc
     7201 ccgaagggat gttgaaaaag caatttacca cggtttcggt cattccccgg ttaaggctta
     7261 catgacaaaa aaagtaataa ctatcgatcc cgaaactccg gtaaggacca ttgagaattt
     7321 gctggtggaa cacaacatag gcaggcttcc cgttataagc ggaaatgagc ttgtgggaat
     7381 agttacccga acggatataa tagccactct gtttggtaat aatagcaaat attggagtaa
     7441 gaagaacaaa gcttccgatt tgtccagatg cagctacaaa ttaaacaacc tggccgacag
     7501 gattaaagac cttcccgaaa gggttgtaag agcactggcc gatgcaggaa gaattgccga
     7561 tgaggagggt tttgtcgcct atgtggtggg aggatttgtc cgtgacctga tacttgggat
     7621 agaacacgtc catgtagttc tcgcccgaga cctcctcgtc gtacaggcca taccacacgt
     7681 cggtgtagat gaagtcgacg cccttgatgc actcgatgtc gtcgg
//
LOCUS       DAOOJC010000055         7661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_8081_length_7661_cov_3.496713, whole genome shotgun sequence.
ACCESSION   DAOOJC010000055 DAOOJC010000000
VERSION     DAOOJC010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8081_length_7661_cov_3.496713"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..853
                     /locus_tag="PLA01_02625"
     CDS             107..853
                     /locus_tag="PLA01_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00226.1"
                     /translation="MYETIPLQFRNVEYEMHCVKVLDKNDIKKIYLEKDVGGGKVSIF
                     AHSNPEISIFSSGRIMGDDLYAGFEINETYYNLGKFYMDVYDERIKAEENWASEIDFC
                     GIKLLRICGAMGLDYYHTNYYSFHDGKPGTFLITSGRGIERDIDNDGKEEIISSSGGS
                     TSSTFIYKYEKGCIKECHLNPVLDNAIAVFYDENTNNFLAYYPDGKFKSKRFKYNGEE
                     MLLLDMEYRPGQFQNYLERVKERLLLKKKE"
     gene            948..1961
                     /locus_tag="PLA01_02630"
     CDS             948..1961
                     /locus_tag="PLA01_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HPD00227.1"
                     /translation="MDNNIKIEKAVLHILDNNVGIPVLSDKELETDNETNSFLEKHIE
                     KLLNDSNLKEAKFIGDSNSMRDLCRHLADNSGDFLQASIYTAQRLFDIMTKNVDIVPA
                     DLICCLFNYVGQPYFGILKLNYKTGFTHYVEKVEEGNVNTIIRHRTLLPSDSQKIDEC
                     AFINLNSFDIKLIEKVYEINGEKEFYLSRLFLICSTNLSDNEKLKIIDKVTQKINKKF
                     FDEDFNNIAKLKKAVSESLEETSSIELNTIADEVFENNLEIKSEYIAEIQKAGLDEET
                     ISVPQKLAERKYRTSKIKTDTGVEINFPSSYYNDKDKLEFINNQDGTVSILIKNVNKI
                     TNK"
     gene            2272..3282
                     /gene="gap"
                     /locus_tag="PLA01_02635"
     CDS             2272..3282
                     /gene="gap"
                     /locus_tag="PLA01_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPD00228.1"
                     /translation="MAVKVGINGFGRIGRLVFRASLNNPNVEVVGINDPFIDLEYMQY
                     MLKYDTIHGQFKGEISEDNGKLVVNGKKISVYASMDPAEIPWSECGADYIVESTGVFT
                     TTEKASAHFKGGAKKVVISAPSADAPMFVMGVNNDKYTKDMKVVSNASCTTNCLAPLA
                     KVIHDNFGIIEGLMTTVHATTATQKTVDGPSKKDWRGGRAAAGNIIPSSTGAAKAVGK
                     VIPELNGKLTGMSFRVPTLDVSVVDLTCRLEKAATYDEIKAAVKKASENELKGILGYT
                     EDMVVSSDFIGDARTSIFDAGAGISLNSNFVKLVSWYDNEWGYSNKVVDLIVHMASVD
                     AK"
     gene            3422..4615
                     /gene="pgk"
                     /locus_tag="PLA01_02640"
     CDS             3422..4615
                     /gene="pgk"
                     /locus_tag="PLA01_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPD00229.1"
                     /translation="MAMMNKKSIEEIDVKGKKVIVRVDFNVPLDENGKITDDKRIVGA
                     LPTIKYLVDNGAKTILVSHLGRPKDGFEDKYSMKPTALRLGQLLGKEVIMAKDVIGPD
                     AKEKAAALKDGEVLMLENVRFHKEETKNDAAFAKELSELAEIYVNDAFGTAHRAHAST
                     AGLADYLPAVCGYLIQKEIDIMGKALSNPERPFVAILGGAKVSDKIGVIENLIGKVDY
                     LIVGGGMAYTFFKAKGYGIGTSICEDDKVELAKSLLEKAESQGVKLMLPVDNAVGKEF
                     KNDTESKVVASNQIPDGWMGMDIGPETIKQYSEVIKKAKTVVWNGPMGVFEFANFANG
                     TKEVAKAVAESGAISIIGGGDSAAAVEQLGFADKITHISTGGGASLEFLEGKVLPGID
                     VLMDK"
     gene            4631..5386
                     /gene="tpiA"
                     /locus_tag="PLA01_02645"
     CDS             4631..5386
                     /gene="tpiA"
                     /locus_tag="PLA01_02645"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPD00230.1"
                     /translation="MSRKIIAAGNWKMNKTAKEAVEFVNALKGRVADADSEVVVGVPF
                     VCLPGVIEAVKGSNIKVAAQNMHWEEKGAFTGEVSGPMLADLGVDYVIIGHSERRQYF
                     GETDETVNKKAHAAFKYGLKPIICVGESLTQREQGITAELVRYQVKIALLDLSADQVK
                     NTVIAYEPIWAIGTGKTATSEQAEEVCAIIRGCIKELYGSDVAEAVRIQYGGSVNAAN
                     ASELFNMPDIDGGLVGGASLKLDDFEKIAKYNK"
     gene            5514..5678
                     /locus_tag="PLA01_02650"
     CDS             5514..5678
                     /locus_tag="PLA01_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00231.1"
                     /translation="MKLLKSDGGSMPPELLLSRISRSEITKKRWGFNTPGAFVKWDFP
                     KSKERKVLAF"
     gene            5734..7272
                     /gene="gpmI"
                     /locus_tag="PLA01_02655"
     CDS             5734..7272
                     /gene="gpmI"
                     /locus_tag="PLA01_02655"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPD00232.1"
                     /translation="MNKRLVTLIILDGFGINEREEGNAIKAANKPNIDRFMSQYPNTR
                     IRTSGLDVGLPEGQMGNSEVGHTNIGAGRIVYQELTRITKSIKDGDFFEKKEFLDAID
                     NCRKNNSKLHLFGLLSDGGVHSHNTHLYGLLELAKKQNFNNVYVHCFFDGRDVPPDSA
                     KGYVEELESKLKEIGVGRIASVMGRYYSMDRDKRWERVKLAYDAMVLGKGLTASSAVE
                     AVEESYKREEFDEFVKPTVIMKGDKPVATIDKNDSIIFFNFRPDRAREITRTFTDVDF
                     DGFERGKGYFPVFFVCMTQYDVTIENAVVAFKPESLTNTFGEYISKHGFKQLRIAETE
                     KYAHVTFFFNGGVEAVYDGEDRVLINSPKVATYDMKPEMSAYEVTDSVLECINSKKYD
                     VIILNYANPDMVGHTGVFNAAKSAIEAIDECLGKVVPAVLDQNGVVFITADHGNSEQM
                     VDYETSSPFTAHTTNPVPFIVMGLGDIELKEGRLADLAPTMLGILGLEKPAEMTGESL
                     IKKK"
     gene            7425..>7661
                     /locus_tag="PLA01_02660"
     CDS             7425..>7661
                     /locus_tag="PLA01_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00233.1"
                     /translation="MKKSLFGIKNQVTNLKDFYNMGSQSLANILKERISGWFIAFSIV
                     NMIVLLIQASGLLPVTYRGLLGILEIGLSIFLSYR"
BASE COUNT         2556 a         1148 c         1792 g         2165 t
ORIGIN      
        1 agtttctcct gatttgtcaa cgaccttttg aattgacttt gtattttctt ctataaatcg
       61 ggtgagttca agcccttctg tcgccaatac agaaaacagg gaggatgtgt acgaaactat
      121 accgttgcaa tttagaaatg ttgaatatga aatgcactgt gttaaagtgc ttgataaaaa
      181 tgatataaaa aagatttatt tggaaaagga tgttggcggc ggtaaggtat ccatatttgc
      241 ccattcaaat cctgaaataa gtattttttc ttccggaaga attatgggtg atgacctgta
      301 tgcgggcttt gaaataaatg aaacttacta taatttgggt aaattttata tggatgtcta
      361 tgatgaaagg ataaaggcag aggaaaattg ggctagcgaa attgattttt gcggtattaa
      421 actgttaaga atatgtggtg ctatggggct tgattattat cataccaatt attattcatt
      481 tcatgatggg aaacccggta cttttttaat tacgagcgga aggggcattg aaagagacat
      541 agataatgac ggaaaagaag aaataatttc ttcgtcggga gggtcaactt catcgacctt
      601 catatataaa tatgagaaag gctgcatcaa agagtgccac cttaatccgg tgcttgataa
      661 tgctattgct gttttttatg acgaaaatac aaacaatttt ctggcttact atcccgacgg
      721 gaaatttaag tcaaaacgat ttaaatataa cggtgaggaa atgttattat tggatatgga
      781 gtacagaccc ggtcaattcc aaaactattt ggaacgggtc aaggaaaggc tgttattaaa
      841 aaagaaggag taaaaagcag gcatagctat cgaaaacacc gggtggatat gcaagatacc
      901 gttatgatat aatgtttcgt gcaaaaaaat agaagtgagg tcatagtgtg gacaacaata
      961 taaaaataga aaaagcagtg ttacatattc tggacaacaa tgtcggtatt cctgtgctct
     1021 cggacaagga attggaaacg gataatgaaa caaacagttt tttggagaag cacattgaga
     1081 agttgttaaa tgacagcaat ttaaaggaag ccaagtttat aggagattcc aatagcatga
     1141 gggatttgtg ccggcacttg gcagataatt ccggcgattt tttgcaagca agcatttata
     1201 ctgctcaaag actgtttgat attatgacga aaaatgttga tatcgtaccg gcagatctga
     1261 tatgttgcct ttttaattat gtcggtcaac cttactttgg tattttgaag ttaaattaca
     1321 agaccggttt tactcattat gttgaaaagg tcgaagaagg caatgtcaac accattatac
     1381 ggcacaggac ccttttgccg tcggattcac aaaagataga tgaatgtgcc tttataaact
     1441 taaatagttt tgacattaag cttattgaaa aagtatatga aataaatggc gaaaaggagt
     1501 tttatctgtc caggcttttt ttgatttgtt caacaaatct ttcagacaat gaaaaactga
     1561 aaatcattga taaagttacc caaaagataa acaagaaatt tttcgatgaa gattttaata
     1621 atattgcaaa gctaaaaaaa gcggtatcgg aaagccttga agagacaagt tctattgaac
     1681 tgaatacaat tgcagatgaa gtttttgaaa acaatctgga gataaaaagc gaatatattg
     1741 ctgaaataca aaaggcaggt ttggatgaag aaacaatatc cgtaccacaa aagcttgcag
     1801 aaagaaaata caggacttct aaaattaaaa ccgatactgg tgtggaaata aactttccgt
     1861 caagttatta taacgataaa gacaaactgg agtttattaa caatcaagac ggaaccgtat
     1921 caatactaat aaaaaatgta aacaagataa ctaataagta aaacagaaat catatgacta
     1981 aaatttaatt atctgataat aataatagtg attattacaa aaaaacaaat catatgacat
     2041 gaattgtttt atataaaagc cggtaaagaa ggaccgggac agctaaagca aaagtttgtt
     2101 aaaaaattat ttatttttta cttttggtat tgaaaataga aatgaaataa tataaaatgg
     2161 ttgatgttat atgataatct tgccagttgg tgaatttatt actgcattca tactatctgg
     2221 tgactgatat aagcattatt agattattta tttacatagg aggcgatatt aatggcagta
     2281 aaagtaggta ttaatggttt tggacgtatc ggacgtctgg ttttcagggc ttcgctcaac
     2341 aatcctaatg ttgaggttgt aggtatcaac gatccattca ttgacctgga atatatgcaa
     2401 tatatgttaa agtatgacac aattcacggt caattcaaag gtgaaatttc cgaagataac
     2461 ggcaagcttg ttgtaaatgg aaagaaaata agtgtttatg ctagcatgga tccggctgag
     2521 attccttgga gcgaatgcgg agcagactat attgttgaat caacaggagt attcacaaca
     2581 acagaaaagg cttcagctca ctttaagggc ggtgccaaaa aggttgtaat cagtgctccg
     2641 tctgctgatg caccgatgtt tgtaatgggt gttaacaatg acaagtatac taaggacatg
     2701 aaggttgttt caaatgcttc atgtacaaca aactgtttag cacctcttgc taaagttatt
     2761 catgacaact ttggaattat cgaaggatta atgacaactg ttcacgctac aactgcaaca
     2821 cagaaaacag ttgacggtcc ttcaaagaaa gactggagag gcggccgtgc tgctgctggt
     2881 aatataattc cttcttccac aggagctgca aaagctgttg gaaaggttat tccggaattg
     2941 aacggcaaat tgacaggtat gtccttcagg gttccgactc ttgacgtatc agttgttgac
     3001 ttgacctgcc gtcttgaaaa agctgctact tatgatgaga ttaaggcagc tgttaaaaag
     3061 gcttcagaaa acgaattaaa gggaattttg ggatacacgg aagatatggt tgtatcatca
     3121 gacttcattg gtgatgcacg tacatcgatt tttgatgcag gagcaggtat ttcattgaac
     3181 agcaacttcg ttaaactcgt ttcatggtac gacaatgaat ggggttattc aaacaaagta
     3241 gttgatttaa tagttcatat ggcttctgta gatgcgaaat aattgtctgt tttataaaat
     3301 ggcaattaaa aaaggttcgg tttcgaccga acctttttgt gaataagttc atagacactt
     3361 agagcaatct ttattattca ataatactat agtgttcaat aagtaaggag cgtgaagaat
     3421 tatggcaatg atgaacaaga aatctattga agaaattgac gtaaagggaa aaaaggtaat
     3481 agtcagggtt gattttaacg tgcccttgga tgagaacgga aaaatcactg atgacaagag
     3541 aatagtcggt gctcttccaa ccattaagta cttagtagat aatggagcaa aaaccatatt
     3601 ggtttctcat cttggaagac cgaaagacgg ctttgaggac aaatacagta tgaagccaac
     3661 agctctcaga cttggccagc tcctcggcaa agaagtcatt atggcaaagg atgttatcgg
     3721 gccggatgcg aaagagaaag ctgctgcatt aaaagacgga gaagttttga tgctcgagaa
     3781 tgtaagattc cacaaggaag agactaaaaa tgatgccgct tttgcaaagg aattgtctga
     3841 gttggctgaa atttatgtaa atgacgcatt tggaactgct cacagggccc acgcttcaac
     3901 agcaggtctt gcggattatt tgccggcagt atgcggttac ctcattcaaa aggaaattga
     3961 tataatgggc aaggctttat caaatcctga aagacctttt gtagcaatat tgggcggtgc
     4021 caaggtttca gataaaattg gtgttatcga aaacttaatc ggcaaggttg attatctgat
     4081 agttggcgga ggtatggctt ataccttctt taaagcaaag ggttacggca taggcacttc
     4141 catatgtgaa gacgataaag tggaactggc aaagagcttg cttgaaaagg cagaaagcca
     4201 aggcgttaag ctgatgcttc ctgtggacaa tgcggtagga aaagagttta aaaatgatac
     4261 tgaaagcaag gttgttgctt caaatcagat tcctgacggt tggatgggaa tggacattgg
     4321 tcctgaaaca atcaagcagt attccgaagt aattaagaag gccaaaactg tagtttggaa
     4381 tggaccgatg ggagtatttg agtttgcaaa tttcgcaaac ggaacaaaag aagtggcaaa
     4441 agctgttgcc gagtccggtg cgatttcaat aattggtggc ggagattctg ctgcagctgt
     4501 tgagcaactg ggttttgcag ataaaataac tcacatttct actggtggcg gtgcatcatt
     4561 ggagtttctg gaaggaaaag tgctgccggg aatagatgta ttaatggata aataagggga
     4621 gaagatgtaa atgagcagaa aaattattgc agctggaaac tggaaaatga ataagacagc
     4681 taaagaagct gttgagtttg tgaatgcttt aaaaggcaga gttgcagatg ccgattctga
     4741 ggtagtagta ggggtgcctt ttgtatgttt gcctggcgtt atagaagctg ttaaagggtc
     4801 aaatatcaag gttgccgcac aaaacatgca ctgggaggag aaaggtgcat ttaccggaga
     4861 agtttccggg ccgatgctgg ctgaccttgg ggttgattat gtcattatag gccactctga
     4921 aaggagacag tacttcggtg aaaccgatga gaccgtcaat aaaaaggctc atgctgcatt
     4981 taagtatgga ttaaagccga ttatttgcgt aggtgagtcc ctgactcaaa gagagcaagg
     5041 aattacagcc gaactggtaa gatatcaggt aaaaattgct ttattggatc tttcggcaga
     5101 tcaggtcaaa aatactgtaa ttgcatatga accgatttgg gcaataggta ccggtaagac
     5161 tgctacaagc gagcaagcag aggaagtttg tgcaataata aggggctgta ttaaagagct
     5221 ttatggcagc gatgttgcag aagctgtaag gattcaatat ggcggaagtg ttaatgcggc
     5281 taatgcatcg gaacttttca acatgcccga tattgatgga ggacttgttg ggggagcaag
     5341 tctcaaactt gacgactttg aaaaaatagc aaagtataat aagtaagtgc ttaatttata
     5401 aatcatgcat tacttagcgg ttagttttag taattgattt tgattagtgg attttatgct
     5461 aagatagctc catcaataaa cgtggggcta ttttattagt aggatttcac gaagtgaaat
     5521 tactaaaaag cgatgggggt tcgatgcccc cggagctttt gctgagtagg atttcacgaa
     5581 gtgaaattac taaaaagcga tggggcttta atacccctgg agcttttgtt aagtgggatt
     5641 ttccgaagtc aaaggaacga aaagttttgg cattttgagt caagaagacc gtaaacttga
     5701 ttcattctta ttaaataaat gaggtgtaat acaatgaata aaaggttagt aactttgatt
     5761 atattagacg gttttggcat aaatgaaaga gaagaaggga atgccataaa agctgccaat
     5821 aaacccaata tagacagatt tatgagtcag tatcccaata cccgtattcg caccagcggc
     5881 ttggatgtgg ggttgccgga gggccaaatg ggtaattccg aggtaggtca taccaatatt
     5941 ggagcaggaa gaatagtgta ccaggaactg acaaggataa ccaagtctat aaaagacggt
     6001 gatttttttg aaaagaaaga atttcttgat gcaatagaca actgcagaaa gaataattca
     6061 aagctgcatc ttttcggact gttatctgac ggaggtgttc acagtcacaa cacccatttg
     6121 tatgggcttt tggaactggc taaaaaacag aattttaata atgtctatgt tcactgcttt
     6181 tttgacggaa gggatgttcc ccccgatagt gcaaaaggtt atgttgaaga gcttgagagc
     6241 aaactcaagg aaatcggcgt tggcagaata gcttcggtta tgggaaggta ctattccatg
     6301 gacagggaca agaggtggga aagggtcaag cttgcatatg atgccatggt tttgggaaaa
     6361 ggcttgacag cttcgagtgc agtagaggca gtggaagaat cctataaaag agaagagttt
     6421 gacgaatttg taaagcctac ggtaattatg aaaggtgata aaccggtggc aaccattgat
     6481 aagaatgact cgataatatt ctttaatttc aggcccgaca gggcaagaga aataacaaga
     6541 acctttaccg acgttgattt tgacggtttt gaaagaggaa agggttattt cccggtgttt
     6601 ttcgtatgta tgacccaata tgacgtgaca attgaaaacg ctgttgtggc tttcaagcct
     6661 gaaagcctta ccaatacttt tggcgaatat atcagcaagc atgggttcaa acagcttcgt
     6721 attgcagaaa cggagaaata tgctcatgta accttcttct ttaacggcgg agtagaagca
     6781 gtatatgatg gggaggacag ggtattgatt aattctccta aggttgcgac ctatgacatg
     6841 aagcctgaaa tgagtgcgta tgaagtaact gacagcgtac ttgagtgcat aaattcaaaa
     6901 aaatacgacg taataatact taactatgca aatcctgaca tggtaggaca cactggagtg
     6961 tttaatgcag caaaatctgc aattgaggct atagatgaat gccttggaaa ggtggttcct
     7021 gcggtgcttg accagaatgg ggtagtgttc atcactgcag accatggaaa ctccgaacag
     7081 atggttgatt atgaaacgag cagtcctttt acggcacata caacaaatcc tgttccgttt
     7141 attgtgatgg gacttgggga cattgagctt aaagagggaa gattggctga ccttgcacct
     7201 acaatgcttg gtattttagg gctcgaaaag cctgctgaaa tgaccgggga gtcattgata
     7261 aaaaagaaat aagctcttgt agacgtgcca tcaaagaaaa gtgtgtactc cggaaaggtt
     7321 attacttgag aaaaattgca ctataatata taataattca taaaaaggac aaaatttgac
     7381 ctgcttggtg agtgcgtatc agtagcaaaa aaaggaaggt cagtatgaag aaatcattgt
     7441 ttggtataaa aaatcaagta acaaatctga aggattttta taatatgggt tcacagtcat
     7501 tggcgaatat tttgaaggag agaatttccg gatggtttat agcctttagt atagttaata
     7561 tgattgtttt attgatacag gcttccggtt tactgccggt aacatatcgg ggtttgctgg
     7621 gaatattgga aataggtctt tcgatatttc tctcctatcg t
//
LOCUS       DAOOJC010000056         7600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_8154_length_7600_cov_3.584626, whole genome shotgun sequence.
ACCESSION   DAOOJC010000056 DAOOJC010000000
VERSION     DAOOJC010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8154_length_7600_cov_3.584626"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PLA01_02665"
     CDS             <1..661
                     /locus_tag="PLA01_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HPD00234.1"
                     /translation="MMSIMADRPQVEEMIARSGLNLSLANINSPEQTVIGGKQDQILR
                     MEEYLSQNGVLFKRLGVSHAFHTELVSQAAESFYSKIKDMVFDVPKTKVMACHRVDFY
                     PDTKDGMSSMPSLLKDQINSVVCFKDAVLKLYEKGVRVFIEAGPSSVLTNLVKVILDG
                     KDVRVVASNSKRKDSVEGYKEALAELFACGMDVYTLPSKKDMGCVRKARKRTINTLPC
                     P"
     gene            610..5772
                     /locus_tag="PLA01_02670"
     CDS             610..5772
                     /locus_tag="PLA01_02670"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPD00235.1"
                     /translation="MRSESEKKNNKYSAVPLKKDSIVYSGVSVGLPGTYKHVFSDNNF
                     DLIFEGKNLIEMLTDEEASSMVDLNITRLIKNEKETAFKKLLSINDVIRLAGKLGKID
                     MLNDYKIDEKVLRQMTATVCAGVAAGYEALKDAGIPLVREHIKTSTGTSLHGRLVLPE
                     DMQEDTGIIFANGFLAVEPYISEVSRYVASKFGAKSRNDLTDFYEKVIERVSDNEVRK
                     LLSDWFVFHYSKLLGDLGEEDIYVFNRDFMALISSQANNRLAQFIGARGPNFHISAAC
                     SSTATAVTIAEDLIRGGHARRMIVIGAENATSKTTLPWVGGCFLSTGAATTSSDVFEA
                     AVPFDKRRNGMILGAGALGLVMEKEEDVERRGMNGICRILGTHSFNTAGHQSKIDKNA
                     YCTELDRFMTRMEREHGFDRNSIAPKTVYYSHETFSPREGGCAQTEAAALKRTFGEKY
                     KDIKVINTKGMTGHTMAASIEEAIAAKALQYQRIPLVVNYKEPDPDLEGLNLSRGGPY
                     SFEYVIRMVAAYGGQGNYHLLQRIACTEDRIFDEKRYDQWLCEVTDSAEAKLNNCGRI
                     LVAEAAEGFKEPMEKPSFLSPGVPGACMAEAKPAEKNDSPVVKSVSAQKPNKESTAVQ
                     SMNTGNSNAESSVIKSTNTGNTNAESPIAQSHDMTDEILGIFSEITKYPKEMLDLTME
                     IEADLGIDTVKQATILSIISEKYGILQEESLRFSDYPTIGHILDMVQQKVLSEGKFEE
                     SPMEYQKPTEKKPIAENPVNESRDEKTEKPGIGSIFTSYTDEAVVKIPGKNLEDEVFE
                     IVSRVTNYPMEMLEKDMELEADLGIDNISYSKLLSMLGERFDTLESGKLLSCKAGTIG
                     EIIECVKKHHSDTSGLGCKDTGREDGMKCLVKNSSGFEKNNEERELCLQIPVFVEEQT
                     GEKDLDLKDCKIWVIGDDKLSVERVSGYLKKRKLDVEEFLFKPFEEDEHLVKRVRDFI
                     CREGNVIIDCSHIAANVEFDSIDRQEQEGWLFLGSEARFIFYKELSQKLKDPKIRIVC
                     AVSMDGWHGYSNREEISIDPFYGALSGFYKGLGKEWSNSVVKVVDLGVNEGGKLEDKV
                     IEMLCIETESSSKDCEIGYRDGKRYVFRVDYLDRTNFKEADIPQNPHFLITGGGHGIT
                     SEITRGLSRRFKAKFTIIGRTELPDNIEELSKLDKEKLDNVKEDIKTRLEETHGRVTP
                     AMLEQEVRALENSLSIKRLMEGIESDGNEVLYLSCDVRCDEDLKRTIEQAVGKNGPVN
                     ILIHGAGIEKSRLLSQKKREEFREVFSVKAEGMCNLYRLLDLKELKAAFAFSSISGRF
                     GNEAQIDYCSANSFLSSFMAMVRAKHKHIYALSIAWSGWKDLGIAWRNEYVRNHSEEM
                     GLNLIEPSRGTAEFINILTGNLETREIIVSKGLTRFTGEKMSRRVYNDKPMIDWVSIR
                     DGRVEKAFRVFSPKRDAIIDHHRLGTVPLGPAVAYMEMGAQLHTIMFGESKQYCFRNV
                     RIDKPLKFFREEPKEVVVQLKEDRERRCFDMEACTYLDNSYANLGLISLSSMNVSDDP
                     GEYQHLLSITELENEPMEEGYTRESLQKFSEENKNSIRLGTLFIDDRKENNVFKYNKN
                     GAVISMVMPQEEKTNKKYNLDKLLINPAFMDSVFQVCGIHSQVNADEVYLPWEAEEFG
                     VINAPREIMNYKAYSRLKYKSGDTKVYDVIMLNERNEVCYYAKSVKMRVIHL"
     gene            5769..6506
                     /locus_tag="PLA01_02675"
     CDS             5769..6506
                     /locus_tag="PLA01_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HPD00236.1"
                     /translation="MIKNGKEEQGEVERLLKTHLGSLTGKYVFSFHVVDMREINRKVH
                     TRELTLSSLLSDKELQYLLGLKNKKNYIQWISGRYAVKSAFFKYKYEEQSVMDLKCVD
                     VLRGEDSAPYILQHPDICVSITHSFPYCIGVVSNRKIGIDIEKVFNPKDALIKRYFSE
                     GEREHLESLLGTEEFSKKATILWTRKEAVSKVLKLGMNMDFKTVDVLSDEIYSIGEDK
                     EAISMSSFTCNDFCLSVAVGRCCNLKV"
     gene            6741..7274
                     /locus_tag="PLA01_02680"
     CDS             6741..7274
                     /locus_tag="PLA01_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPD00237.1"
                     /translation="MNVLNGDCESFSALVQLYENRIYNFLLKLTLSKEDAEEILQDVF
                     LRVYKYLHKYDDKLNFSAWIYKIALNTYKSSYKKKKKDFHNVCCEQIVDSICSAEYDP
                     EHSYELKEKYTEIVKILDCLKTEQKVAFLLKHIQGFTYKEIGHIMGISEQAAKMKVQR
                     AKQILCDRYYKFNFRGV"
     assembly_gap    7374..7383
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7384..>7600
                     /locus_tag="PLA01_02685"
     CDS             <7384..>7600
                     /locus_tag="PLA01_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00238.1"
                     /translation="KKCRCYYESLEFANKYASGEVLLEQSIHNSVMSRIDKTRYLNKK
                     YSILRGIKKLTPAFKHTLTAIVCCLIFV"
BASE COUNT         2461 a         1241 c         1877 g         2011 t
ORIGIN      
        1 tatgatgagt atcatggctg accgcccaca ggttgaagag atgattgcaa ggagtggtct
       61 taacttaagc cttgctaata ttaattcacc ggaacagacg gttataggag gaaaacagga
      121 tcaaatcctg cgaatggaag agtatttgtc ccaaaacggg gttttgttta agaggcttgg
      181 tgtatcccac gcatttcata cagagcttgt atcccaagca gcagaaagct tttacagcaa
      241 aatcaaggac atggtgtttg atgtgccaaa gaccaaggtt atggcttgcc acagggtgga
      301 tttttatccc gataccaaag acggaatgag cagcatgcct tcattgctga aagatcaaat
      361 aaattcggtg gtctgcttta aagatgctgt attaaagctc tatgaaaaag gcgtgagagt
      421 ttttattgag gcaggtcctt catcggtgct tacaaacctt gtaaaggtta tccttgatgg
      481 caaggatgtc agggttgtgg cgtcaaacag taaaagaaag gattccgtag agggatataa
      541 agaggctttg gcagagcttt ttgcctgcgg tatggatgtt tatactcttc cttcaaaaaa
      601 agatatggga tgcgttcgga aagcgagaaa aagaacaata aatactctgc cgtgcccttg
      661 aagaaggaca gcattgtgta tagtggggtt tctgtcggat tgccgggtac atacaagcat
      721 gtattttcgg ataacaactt cgatctgatt tttgaaggta aaaaccttat agaaatgctg
      781 accgatgaag aggcaagcag catggtggat ttaaatatca caaggcttat aaaaaacgaa
      841 aaggaaaccg cttttaagaa actgttatca attaatgatg ttataaggct tgccgggaag
      901 cttggcaaga ttgatatgct aaacgactat aaaattgacg agaaggtact gaggcagatg
      961 accgcgactg tgtgtgcggg agttgctgca ggatatgagg ctctgaagga tgcgggtatt
     1021 cctcttgtaa gagagcatat aaagacttca acaggaactt ctctgcacgg aaggcttgtg
     1081 cttccggagg acatgcagga agacaccgga ataatttttg ccaacgggtt cttagcggtg
     1141 gaaccttaca taagtgaggt ttcaaggtat gttgcatcga aattcggagc aaagtcaaga
     1201 aatgacttaa ctgattttta tgaaaaggtg attgagaggg taagcgacaa tgaagtgagg
     1261 aagcttttat ccgattggtt tgtatttcac tactcaaagc ttttaggaga cttgggcgaa
     1321 gaagatattt atgtatttaa tcgtgatttt atggcattga tatcttcaca ggcaaataac
     1381 aggcttgccc agtttatcgg ggcaaggggg ccgaatttcc acatcagtgc cgcctgttct
     1441 tccactgcca ctgcggttac cattgctgag gaccttatcc gcggcgggca tgcaaggaga
     1501 atgattgtta tcggtgctga aaatgctaca tcaaaaacga ctctgccctg ggtcggtgga
     1561 tgtttcttaa gtactggtgc tgctactact tcttccgatg tcttcgaggc agcggtacct
     1621 tttgataaga gaagaaacgg aatgattctt ggggcaggtg ctctagggct ggtaatggaa
     1681 aaggaagaag atgtagagag aaggggcatg aatggaattt gccgtatttt aggcacccat
     1741 tcctttaata ctgccgggca tcagtcaaag attgacaaaa acgcttattg tactgaactg
     1801 gacaggttca tgaccagaat ggaaagagag catggttttg acagaaacag cattgcaccg
     1861 aaaacggtgt attattccca cgaaaccttt tcgccaagag aagggggctg tgcccagact
     1921 gaagcggcag ctcttaaaag gactttcgga gaaaaataca aggatataaa ggtcatcaat
     1981 actaaaggga tgaccggtca taccatggcg gcatccatag aagaggctat agctgcgaaa
     2041 gccttgcagt atcagagaat cccgctggtg gtaaattata aagagccgga tcccgattta
     2101 gaaggcttga atttatcccg aggcggtcct tacagctttg agtatgttat aaggatggta
     2161 gcagcatatg ggggtcaggg aaactaccac ctattgcaaa gaatagcatg cacagaagac
     2221 aggatatttg atgaaaagcg gtatgaccaa tggctgtgcg aggttactga ctcggcggaa
     2281 gcaaagctta ataattgcgg aagaattctc gtggcagagg cggcggaagg ttttaaggaa
     2341 ccgatggaaa aaccaagttt cttaagcccg ggagtgcccg gggcatgtat ggcagaagcc
     2401 aaacccgcag agaaaaatga cagcccggtt gtaaaaagtg tgagtgcaca aaaaccaaat
     2461 aaagaaagca cggctgtgca aagcatgaat acgggtaatt caaatgccga aagctcggtc
     2521 ataaaaagca ccaatacggg aaatacaaat gccgaaagcc cgattgcaca gagtcatgat
     2581 atgacggatg agatcttggg tatattttcg gaaataacaa aataccctaa agaaatgctt
     2641 gacctgacca tggagattga ggccgatttg ggtattgata cggttaaaca ggccactatt
     2701 ttgtctataa tatcggaaaa atacggaatt cttcaggaag aaagcttaag attttcggat
     2761 tatcctacca tcggacatat actggacatg gttcaacaaa aggttttaag tgagggaaaa
     2821 tttgaggaaa gtccgatgga gtaccaaaaa ccgacagaga aaaaaccaat tgccgaaaat
     2881 ccggtcaatg aaagccggga tgaaaaaaca gaaaagccgg gcattggctc tatatttaca
     2941 tcatacacag atgaggctgt agtaaaaatt cccgggaaga acttagaaga tgaagttttt
     3001 gaaattgtgt cccgggtgac caactatccg atggaaatgc tggagaagga tatggaattg
     3061 gaagccgatt taggcatcga taatataagt tacagcaaat tattatcgat gctcggtgaa
     3121 aggtttgaca cgcttgaatc cggcaaactg ttatcctgca aggccggtac tataggtgaa
     3181 attatagaat gtgttaaaaa acaccatagc gatacttcag gtttgggctg taaggatacc
     3241 ggacgggaag acggaatgaa gtgccttgta aaaaacagca gcggctttga gaagaataat
     3301 gaagaaaggg agctttgcct tcaaatacct gtttttgtgg aagaacaaac cggtgagaag
     3361 gacttggatt taaaggactg caaaatatgg gttatcggcg atgacaagct atcggttgaa
     3421 agggtatccg gctatttgaa aaaacggaag ttggatgtcg aagagttctt gtttaagccc
     3481 tttgaagagg atgagcatct tgtaaaaaga gtccgtgatt ttatatgccg ggaagggaat
     3541 gtaattatag attgttccca tatagctgcg aacgtagagt ttgattcaat tgacaggcag
     3601 gaacaggagg gatggttatt cctgggcagc gaggcaaggt ttatattcta caaggaactt
     3661 tcccaaaagc ttaaagaccc gaaaattaga attgtatgtg cggtatcgat ggatggctgg
     3721 cacggttatt ctaacaggga ggaaatcagt atcgatcctt tttatggggc tttgagcggt
     3781 ttttacaaag gacttggaaa agaatggagt aattcggttg tgaaagttgt cgacttggga
     3841 gtgaatgaag gcggcaagct cgaggataaa gttatcgaga tgttatgcat tgaaaccgag
     3901 tcttcttcaa aggactgtga aatcggatac agggatggaa aaagatatgt tttccgggtg
     3961 gattatttgg accgaacgaa ttttaaagaa gcggacatcc cacagaatcc gcacttcctg
     4021 ataacgggcg gaggacatgg aatcacctcg gaaataaccc gggggctttc aagaaggttt
     4081 aaagctaaat ttacaataat cggcagaaca gaattgccgg ataatatcga agagctttct
     4141 aagcttgaca aggaaaagct tgataatgtc aaggaggata ttaaaacaag actggaggag
     4201 acccatggac gggtgactcc cgcaatgctg gaacaagagg ttagagccct tgaaaactcc
     4261 ctatcgatta aaagacttat ggaaggaatt gaatcggacg gcaatgaggt gctgtatttg
     4321 tcctgcgatg tcaggtgcga tgaagatttg aagaggacta tagagcaggc tgtcggaaaa
     4381 aacggaccgg tcaatatcct tatccatggg gccgggattg aaaaaagcag gctgttatcc
     4441 cagaaaaaac gggaggaatt tagggaggtt ttctccgtga aggctgaggg aatgtgcaat
     4501 ctttaccggc ttttagactt aaaggaatta aaagcggcct ttgctttctc atccatatcc
     4561 ggcagattcg gtaacgaagc acagattgac tattgttcgg caaacagttt tctaagcagc
     4621 ttcatggcta tggtgagggc aaagcataaa cacatatatg cattaagtat tgcatggtcc
     4681 ggatggaaag acttgggtat tgcctggagg aatgagtatg taagaaatca ttccgaggaa
     4741 atggggctga atctgattga gccttccagg ggaacggcag agtttatcaa tattctgacc
     4801 ggcaatcttg aaaccaggga gattatcgta agtaagggac ttacacggtt tactggtgaa
     4861 aagatgtcac gaagggttta taacgacaaa ccgatgattg attgggtcag cataagagac
     4921 ggaagagttg aaaaggcatt cagggttttt tctcccaaaa gggatgcaat tatcgaccac
     4981 cacaggctgg gaacagtacc cctcggaccg gcggtggcat acatggaaat gggtgcccag
     5041 ctccatacaa ttatgtttgg cgagagcaaa cagtattgct tcagaaatgt cagaatcgac
     5101 aagcccttaa agttctttag ggaagaaccg aaagaagtgg tggttcaact taaagaagac
     5161 agggaaagac ggtgctttga catggaagca tgcacgtacc ttgacaacag ttacgcaaac
     5221 ttggggttga tatctttaag cagcatgaat gtgagcgatg acccgggaga ataccagcac
     5281 ctgctttcta ttacagagct tgaaaatgag cctatggagg agggttatac ccgagagtct
     5341 cttcaaaaat tcagtgaaga aaacaagaac tctatccggt tgggaacttt gttcattgat
     5401 gaccgaaaag aaaataatgt ttttaaatac aacaaaaacg gtgcggttat ttccatggta
     5461 atgccccaag aggaaaaaac caataaaaag tataatctgg acaagcttct gattaatcct
     5521 gcttttatgg attcggtttt tcaggtttgc ggtatacatt cacaggttaa tgccgatgag
     5581 gtttatcttc catgggaggc tgaagagttt ggtgtgataa atgctcccag ggaaatcatg
     5641 aattataagg cttattcaag attaaagtat aaaagtggag atacaaaggt gtatgatgtc
     5701 attatgctca atgaaagaaa tgaagtgtgc tattacgcaa aaagtgtaaa gatgagggtt
     5761 attcatttat gattaaaaac ggaaaagaag agcaaggaga agttgagagg ctactgaaaa
     5821 cccacctggg ttcgttgaca gggaaatatg ttttttcctt tcatgttgtg gatatgaggg
     5881 agataaaccg caaggtgcac acaagggaat taaccctgtc ttcccttttg tcggataaag
     5941 aactccagta tcttttgggt ttaaaaaaca aaaagaatta tatacagtgg atatcgggaa
     6001 ggtatgcggt aaagtctgca ttttttaaat acaagtatga agagcagagt gtgatggatt
     6061 taaaatgtgt ggacgtgtta aggggagagg attctgcacc gtatatcctg cagcatcctg
     6121 acatttgtgt gtcaattacc cattccttcc cgtactgcat tggggttgtg tctaatagaa
     6181 agataggtat agatattgaa aaagtattta atccgaaaga tgcattgatt aagcggtatt
     6241 tttccgaagg agaaagggag cacctcgaaa gcctccttgg taccgaagaa ttttcaaaaa
     6301 aggcaacaat tttgtggaca aggaaagagg ccgtttcaaa ggttttgaaa ctggggatga
     6361 atatggactt caagacggtg gatgtgcttt cggatgaaat atactctatt ggggaggaca
     6421 aagaagcaat aagcatgtca tcttttacat gcaatgattt ctgcctgtcg gttgcagttg
     6481 gccgatgctg taatttgaaa gtttaacggg atttaaaaca atcatctcta aaattctaat
     6541 ctattctagt ctattctagt ctatttaatt cctgccctta tttaatataa ctcaaaaaaa
     6601 gatatatgtt tttcttcacc tttaattaat tcgccaaaaa aaacgatact ttatattttt
     6661 tttataggta ttatatttat agatagcttt tttaaggagg tttacaagta accgatgccg
     6721 atagagacaa attattggta atgaatgtac taaatggcga ttgtgaatca ttttctgctt
     6781 tggttcagtt atacgagaat cgtatatata attttttgct gaaattaacc ctttcaaaag
     6841 aagatgctga agaaatattg caggatgttt ttctcagggt ctataaatat ttacataaat
     6901 acgacgataa actgaatttt tctgcatgga tttataaaat tgctctaaac acatataaat
     6961 ccagctataa gaaaaagaaa aaagattttc ataatgtctg ctgcgaacaa attgtggata
     7021 gtatatgcag tgcagaatat gatcctgagc attcatatga actcaaagag aaatatacag
     7081 aaatcgtgaa aattcttgat tgcctgaaaa ctgagcaaaa ggtggcattt ttattaaaac
     7141 atatacaggg atttacctat aaagaaatcg gtcatattat gggcatcagt gaacaagctg
     7201 ctaaaatgaa ggttcaaagg gcaaaacaaa tactatgtga tcggtattat aaatttaatt
     7261 ttagaggtgt ttaatatgat ttgcaaatat agttatgagg atatcataaa attgtctgaa
     7321 aatagtcttt cccttgagta tgcaaaaaag ctgaaaacac atcttggggc ttgnnnnnnn
     7381 nnnaagaaat gcagatgtta ttatgaatca ctggagtttg ccaacaaata tgcatcaggc
     7441 gaagtgcttt tggaacagag cattcataac agtgtcatgt caagaattga caaaacccgt
     7501 tacttaaata agaaatactc aatattgcgt ggtataaaaa aactaacccc tgcttttaag
     7561 catacattga ctgctatagt atgctgtttg atttttgtag
//
LOCUS       DAOOJC010000057         7582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_8184_length_7582_cov_3.643284, whole genome shotgun sequence.
ACCESSION   DAOOJC010000057 DAOOJC010000000
VERSION     DAOOJC010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8184_length_7582_cov_3.643284"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1358)
                     /locus_tag="PLA01_02690"
     CDS             complement(18..1358)
                     /locus_tag="PLA01_02690"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HPD00239.1"
                     /translation="MSKIIFPEDFIWGAATAAYQIEGAYNEDGKGESIWDRFSHTPGN
                     VFEGHTGDVACDHYHRYEEDIQLMKELGIKSYRYSISWPRIFPDGKGKPNEKGLDFYK
                     RLTNLLLENGITPAVTLYHWDLPQKLQDIGGWANRDVTDYFTEYSEAIIRNLGDVVPM
                     WFTINEPFIISFLGHLFGNHAPGIKDINVTLQVAHNLLLSHGKVLKIYREMNQKGRIG
                     IALNFSFKYPASKDPADIAAANLAFGVFGRWFLDPLYKGQYPEDVLKYYENMGVPFSY
                     SQEDLKLISQPMDFLAFNYYSSDFIKHDDKQGFASIDPKPGKFEKTEMNWIVYPQGLY
                     DLIMYLDREYGKPNLIISENGAAYKDEINSNGRIEDVKRTKYLIDHLTQVHRAIEDGA
                     NMNGYYLWSLMDNFEWSYGYSKRFGIIHVDHETQKRIIKDSGYWYKETIANNEF"
     gene            1461..2192
                     /locus_tag="PLA01_02695"
     CDS             1461..2192
                     /locus_tag="PLA01_02695"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HPD00240.1"
                     /translation="MEKSIGDIKRVARSHLSGTGLNCVIITFTFYVMVATFFEMGYPG
                     KIIILLILAPLLLGTAIYSVYMVRSGNLADNEKSSILDIPIIGPFGMLYSTKILRSFK
                     ISLKPILFGFKYYFKAIALLLTIILRAALWSLLLIIPGIIKLISSSMCFYIMADKPDI
                     SIREAIRLSDKMMEGYKKKCVLLNLSLIGWVIFAFVTCLLGSVITIPYMLAPNAVFYI
                     ELKEENIQKGVITEDEIGITKIWEY"
     gene            2310..3092
                     /locus_tag="PLA01_02700"
     CDS             2310..3092
                     /locus_tag="PLA01_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HPD00241.1"
                     /translation="MEMTLSKLKRKARFQLSNNGANLAILYLVFLAFYILLFFFLRLN
                     GVELSLIINGPIVLGFAIYFLGIARLEKEPRSKKEKEEKRKEKVKKGIFNLFGMEIKL
                     GKKLNINLDTAHNPYRVSMKYVFFGFKYFINSAALYIWTAFLTLLFTFFLIVPGIIKM
                     IDLSMSFFILADNPRMDIRKVVGLSKKMMKGHRKEFIILLLSFIGWILFSIATLSLGF
                     IITIPYIATTCALFYDELKKESIKNGVIAEDEIETKRLWEYN"
     misc_binding    3180..3420
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3556..4575
                     /gene="pheS"
                     /locus_tag="PLA01_02705"
     CDS             3556..4575
                     /gene="pheS"
                     /locus_tag="PLA01_02705"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPD00242.1"
                     /translation="MKEQLNSIRMQAEQELSNVGSITELENIRVKYLGKKGELTTVLR
                     GMGSLSPEERPVIGQLANEVRAALEAKIENVKDELTRKERIQKLDSEVIDVTMPGKRK
                     MLGKKHPLSIVLDEIKEVFIGMGYEIAEGPEVELDYYNFEALNIPKNHPARDVQDTFY
                     INENILLRTQTSSVQIRVMENKKPPIKIICPGRVYRSDAVDATHSPIFHQVEGLVVDK
                     GVTMGDLVGTLKLFAKSLFGEKTDIRLRPHHFPFTEPSAEVDVSCWACGGSGCRVCKN
                     EGWIEILGAGMVHPKVLEVCGIDPEIYSGFAFGLGVERTAMGRFNIDDMRLLYENDIR
                     FLRQF"
     gene            4643..7030
                     /gene="pheT"
                     /locus_tag="PLA01_02710"
     CDS             4643..7030
                     /gene="pheT"
                     /locus_tag="PLA01_02710"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPD00243.1"
                     /translation="MKAPISWLKDYVDINVSSKELADAMTMSGSKVEGIEVQGEEISG
                     VVVGKILSLEKHPDADKLKVSKVDIGREVIQVVTGAPNVNAGDYIPVALAGATLPGGK
                     KISKGKLRGIESNGMMCSIEELGLTRENFPDAPEDGVYIFNKEEELGKDVKVVFGINE
                     ETIEFEITSNRPDCLSIIGLAREAAATLKTEFKKPVIKLKEEDDDVSKYVSVEVKDTD
                     LCTRYTARVVKNVKIGPSPKWMRDRLSAAGIRPINNIVDITNYVMLEYGQPMHAFDIK
                     DIKGSKIVVRRACDEEILKTLDEQDRKLDSSMLVIADEERAIAVAGVMGGANSEIRES
                     TDTIVFESANFDGTSVRLTAKKLGMRTESSARFEKGLDTENVDAAMNRAAELVEQLGI
                     GTVCKGIIDCYHQKKDERTIKLRVEKINEFLGTTISKEEMINILRMLEFEVDENNMTV
                     KVPSFRPDVEREVDIAEEIVRFYGYNNIEDTLLSGKAATLGKKTFKQSIEDLIKNTMI
                     ACGLSETYTYSITSPKVFDKLNLPADSELRKAVVISNPLGEDYSVMRTTTIPDMLEVI
                     STNYNRRIEGVSLFEMSYVYIPKSLPLSELPYEKPVLTLGMYGGVDFYDLKGVVEELM
                     AKLGIEDYDISPEKNSVIFHPGRTAVLSIKGEYAGVIGEVHPEVAEKFECPERTYIGV
                     IETEKLVKNASTKSEYSPLPKFPAVTRDMALLVKDEILVKQIEDIIRQRAGKILEEIK
                     LFDVYKGKQVPEGMKSVAYSIIFRASDRTLTDEDVNKAMKKILDGIKSSLGAELR"
     gene            complement(7110..7331)
                     /locus_tag="PLA01_02715"
     CDS             complement(7110..7331)
                     /locus_tag="PLA01_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HPD00244.1"
                     /translation="MARRNTPLVPESREALKQFKMECAKEIGHLQYVKENNDHYKGDV
                     PSKINGLQGGPIGGQMVKRMIEMAENQLV"
BASE COUNT         2374 a         1203 c         1706 g         2299 t
ORIGIN      
        1 tgctgctgtg cgaaatatta aaactcattg ttggctattg tttccttata ccaataaccg
       61 ctgtccttta taattctttt ctgggtttcg tggtccacat gtattatacc gaatctcttg
      121 gaataaccgt aagaccattc gaaattgtcc ataagggacc acaaataata tccattcatg
      181 tttgcaccat cttcaatagc cctgtgtact tgtgtcaggt ggtcgataag atactttgtt
      241 ctctttacat cttcaattct tccgttgctg tttatttcat ccttataggc agctccgttt
      301 tcactgataa taaggtttgg cttgccgtat tccctgtcaa ggtacataat gagatcatat
      361 agcccttgag gataaacaat ccaattcatt tcggtttttt caaatttacc gggtttcgga
      421 tctatgcttg cgaaaccctg cttgtcatca tgttttataa aatctgaaga atagtagttg
      481 aaagcaagaa aatccatggg ctggcttatt agcttcaagt cttcctgaga ataagagaag
      541 ggaactccca tgttttcata atatttcagt acatcctcag gatactgtcc tttatacaga
      601 gggtcgagga accaccgtcc aaacactcca aaggccaaat ttgcagcggc aatgtctgca
      661 gggtctttgg aagcagggta tttaaatgag aaattaaggg ctatacctat acggcctttc
      721 tgattcattt ctctatatat ctttaaaact tttccatggg acaataaaag attatgtgcc
      781 acctgaaggg ttacatttat atccttgatt ccgggagcgt ggtttccgaa aagatgaccc
      841 aaaaaggaaa taataaaagg ttcattaatt gtaaaccaca tcggtacgac atcacctaaa
      901 tttctaataa tcgcctcact atattctgta aaataatccg taacatcacg gtttgcccat
      961 cctccaatgt cctgaagctt ttgcggaaga tcccagtggt atagggttac cgcaggggtt
     1021 attccgtttt ccagaagaag gtttgtaagc ctcttataga aatccaaacc cttttcattc
     1081 ggttttcctt tgccatccgg gaagattctt ggccatgaaa ttgagtacct gtatgacttt
     1141 atacctaatt ccttcattag ttggatgtct tcctcatagc gatgataatg gtcgcatgct
     1201 acatcaccgg tatgaccttc aaatacattg cctggagtgt ggctgaagcg gtcccaaatt
     1261 gactcacctt ttccgtcttc gttgtaagca ccttcgatct gataagcggc tgttgcagca
     1321 ccccatataa agtcctccgg gaaaatgatt tttgacataa ttatcacatc cttagtagag
     1381 taataggcta aaaatatgtt aatataatta taaccgataa attaatttaa taccatttta
     1441 attttattgg gggtgtaatt atggagaaaa gtattggaga cataaagagg gtggctcggt
     1501 ctcatttaag cggtacaggt ctgaactgtg ttattattac gtttactttt tatgttatgg
     1561 tagcgacatt ttttgaaatg ggatatccag gtaaaataat tatattatta atcttagcac
     1621 cgcttttact gggtactgcc atatacagtg tctatatggt gagatcggga aatttggctg
     1681 acaacgagaa gtccagtata ttggatatac ctataattgg tccctttggc atgttatatt
     1741 ccacaaaaat tttaagatca tttaaaattt ctcttaagcc tatcttgttc ggttttaagt
     1801 actatttcaa agccatcgca ttgctgttga ctattattct tagggctgct ttatggtcgc
     1861 ttttattgat aatacccgga ataataaaac taataagttc gagcatgtgt ttttatataa
     1921 tggctgacaa acctgatata agcattagag aagcaattcg gctaagcgac aaaatgatgg
     1981 agggatataa gaaaaaatgt gtattactta atttaagtct tatcggttgg gtaatttttg
     2041 cttttgtaac ttgtcttttg ggaagtgtta ttactatccc atatatgtta gcgcccaatg
     2101 cagtttttta tattgagctg aaggaagaga atatccaaaa aggcgttatt accgaagatg
     2161 aaataggtat tactaaaata tgggaatatt agttttttta ttattatgtt gctataattt
     2221 tatttgtttt aagtttggga atgtgttaaa ataaatacta ttaagcttga gatagataat
     2281 tttttggttg tatttggggg taataatcga tggaaatgac tttgagcaaa ttaaaaagaa
     2341 aagctcgttt tcagctcagc aacaatgggg caaatctggc aatactgtat ctggtttttt
     2401 tagcatttta tattttacta tttttctttt tacgacttaa tggggtagaa ttgtcattaa
     2461 taattaatgg cccgattgtt ttggggtttg ccatttattt tctgggtata gcgaggctgg
     2521 aaaaggagcc tagatctaaa aaggagaaag aagaaaaaag aaaggaaaag gtaaagaaag
     2581 ggatttttaa tctttttgga atggaaataa aacttggcaa aaaattaaat attaatcttg
     2641 atacagccca caatccatac cgggtaagta tgaagtatgt attctttggt tttaaatact
     2701 tcataaattc agcagccttg tatatctgga cggccttttt aacattgtta tttacgtttt
     2761 ttttgattgt tcccggtatt atcaagatga tagatttgag tatgagcttt ttcatacttg
     2821 ccgacaatcc tagaatggat atcaggaagg ttgtaggtct tagtaaaaaa atgatgaagg
     2881 gccataggaa agaattcatt attttacttt taagctttat tggctggata ctgttttcaa
     2941 tagcaacttt gtcattgggc tttatcatta cgattccgta cattgcaaca acttgtgcac
     3001 ttttttatga tgaactaaaa aaagaaagca taaaaaacgg cgttattgca gaagatgaaa
     3061 tagaaaccaa aagactatgg gaatataact aactgttttg gatattatgt tttattgtcc
     3121 taattaaaaa tgcagattgt ataatcattg gatttgtgtt ataatttata aaattatatc
     3181 taaaggctat gacagagaga gtagatggtt ttggcagata taagagagaa gataccggcg
     3241 gctgagagta tctttatcag agcaaatcgt tgaagttccc tcttgagctt catcctgaac
     3301 atcagtagct gataagtagg agatgacggt tagcaaccgt tatttgctta aaagtgttta
     3361 taggctattt aagcctataa aaatttgggt ggtaccgcgt agattacctt cgtcccatta
     3421 tttgggatga aggtttttta atttattagc tttagcaaag tgaattgact atatagcgaa
     3481 aaggttcgat tcccatgaga ttttgctaag tgactgaaca ataaaaatga attcatataa
     3541 ggaggaattt aaagaatgaa agagcagttg aacagtatta gaatgcaggc agaacaagag
     3601 ctttctaatg ttgggagtat tacggaactg gaaaacatca gagttaaata cctgggtaaa
     3661 aaaggcgagt tgacaacggt attaagagga atgggttcct tgtcgccgga agagaggccg
     3721 gtgattggcc agttggcaaa tgaggtaagg gcggcactgg aagcaaaaat tgagaatgtt
     3781 aaggatgagc tgaccaggaa agagagaatc caaaagctcg atagcgaagt tattgatgtg
     3841 acaatgcccg gtaagagaaa gatgcttgga aagaaacatc ctttgagtat tgttcttgac
     3901 gagattaaag aggtatttat cggtatgggt tatgagatag ccgaaggtcc ggaggttgaa
     3961 ctggattact ataactttga agcactgaat attcctaaaa accatccggc aagagatgtt
     4021 caggatacct tttatataaa tgaaaatata cttctaagga cacagacatc atcggttcag
     4081 atccgggtaa tggaaaacaa gaaaccgccg attaaaatta tctgtcccgg aagggtttac
     4141 cgctctgatg ctgttgatgc aacccattca ccgatattcc atcaggtgga agggcttgtt
     4201 gtggacaaag gtgttacaat gggggatttg gttggaactc ttaagctttt tgcaaaaagc
     4261 ttgttcggtg aaaaaacgga catcagacta aggcctcatc acttcccgtt cactgagccc
     4321 agtgcagagg tggatgtttc ctgctgggct tgcggaggct caggctgcag agtgtgcaaa
     4381 aatgagggat ggatagagat tttgggagcg ggaatggttc accccaaggt tcttgaggtt
     4441 tgcggcatag atcctgaaat ttacagtgga tttgcctttg gattgggagt tgagaggacg
     4501 gctatgggca ggtttaacat tgacgacatg aggctattgt atgaaaatga tatcagattt
     4561 ttaagacagt tttaaaaacc attgagattt aataactaag atataataat tggaatttat
     4621 ttggaaagga gtaatgcttg atatgaaagc accaataagc tggctgaagg attatgttga
     4681 tataaatgtg agttctaagg aattggcaga tgccatgacc atgtcaggct caaaggttga
     4741 aggaatagag gtgcagggag aagaaatttc aggagtggtg gtaggaaaaa tactgtccct
     4801 tgaaaagcac cccgatgcgg ataagttaaa ggtttcaaag gtagatatcg gaagagaggt
     4861 aattcaggtt gttaccgggg ctcccaatgt caatgctgga gattacatac cggtggctct
     4921 tgcgggggca accctaccag gaggtaaaaa gatatcgaaa gggaagctca ggggtataga
     4981 atccaatgga atgatgtgct cgatagaaga actgggactt accagagaaa attttccgga
     5041 tgcaccggag gatggagtct atattttcaa taaagaagaa gaactaggca aggatgttaa
     5101 ggtagtattt gggataaatg aggagacaat cgagtttgaa ataacatcga acagaccgga
     5161 ttgcttgagc attataggtc ttgcccggga ggctgctgca actttgaaga cagagtttaa
     5221 aaagcccgtc atcaagttaa aggaagaaga cgatgatgtt tcaaaatatg tttcggttga
     5281 agtgaaagat accgatcttt gtacaagata taccgcaagg gttgtgaaaa acgttaaaat
     5341 cgggccttct ccgaagtgga tgagggacag gcttagtgca gcaggtatca gacccataaa
     5401 taatattgtt gatataacaa actatgtaat gctcgaatac ggacagccaa tgcatgcttt
     5461 tgacatcaag gacataaagg gaagcaaaat tgttgtaaga agggcttgtg acgaagagat
     5521 attaaaaacc cttgatgaac aggacagaaa gcttgattca tcaatgctag taatagcaga
     5581 tgaagaaagg gctattgcag tagccggtgt tatgggaggt gccaattcgg aaatcagaga
     5641 aagtaccgat acaatagtat ttgagtctgc aaacttcgat ggaacgtccg taaggcttac
     5701 ggcaaagaag cttggaatga ggacggagtc ttcagcaagg tttgagaagg ggcttgatac
     5761 ggaaaatgtt gatgctgcta tgaacagggc agcagagctg gtggaacagc ttgggatagg
     5821 tactgtttgc aaggggataa ttgattgtta tcatcaaaag aaagatgaac ggactataaa
     5881 actccgagtg gagaaaataa acgaatttct gggtacaaca atttcaaagg aagaaatgat
     5941 aaacatattg aggatgcttg agtttgaagt cgatgaaaat aacatgactg taaaggttcc
     6001 aagctttagg cctgatgttg aaagggaagt ggatattgcg gaggagattg tgcggttcta
     6061 tggttacaac aacattgaag atacgctttt gtccggcaag gctgctactc tcggcaagaa
     6121 aacctttaag caaagcattg aggacttgat taaaaataca atgattgcat gtggtttgtc
     6181 agagacttat acctattcta ttaccagtcc aaaggtgttt gataaactga acctcccggc
     6241 ggatagcgag cttcgtaagg ctgttgtcat ttccaatccg ttgggtgaag actatagcgt
     6301 gatgagaacc actacaatcc ctgatatgct tgaggttata agcactaatt acaatagaag
     6361 gattgaaggt gtaagtctgt ttgagatgtc ctatgtgtat attcccaaga gtttgccctt
     6421 gagtgaactt ccttacgaaa agccggtgct cacattagga atgtatggcg gtgttgactt
     6481 ctacgatttg aagggagtcg tagaggaatt gatggcgaag cttggaattg aagattatga
     6541 tatatctcct gagaaaaata gtgttatatt ccatccggga aggacggcag tattgagtat
     6601 aaagggcgaa tacgcaggag taatcggaga ggttcatccg gaggtggctg agaagttcga
     6661 atgccctgaa agaacttaca ttggggttat cgagacagaa aagctggtaa agaatgcatc
     6721 gacgaaatcc gaatacagtc cccttcctaa gttccctgca gttacaaggg atatggcatt
     6781 gcttgtcaaa gatgagatac tggttaagca gattgaggat attataagac aaagagccgg
     6841 aaagatactt gaagaaataa aactctttga tgtttacaag ggtaagcagg ttcccgaagg
     6901 gatgaagagt gttgcttatt ccattatatt cagggcttcc gatagaacct tgactgacga
     6961 ggatgtcaac aaggcaatga agaaaattct ggatggcatt aaatcaagcc ttggagcaga
     7021 gctcaggtag aatccgtata taatttatgc taaaaagcag ctgattaaaa tggagtcaga
     7081 gaacggtttt ttctctgact ccaatatatt tataccaatt gattctcagc catttcaatc
     7141 attcttttta ccatctggcc accgatgggt ccgccctgca atccatttat cttagaaggt
     7201 acatcgccct tgtaatggtc attgttttct ttaacatact gcaaatgtcc gatttccttt
     7261 gcacattcca ttttaaactg ttttagagct tctctgcttt caggaaccaa tggtgtgttt
     7321 cttcttgcca tttttattca ccaacctttt aagaattaat tttgtatgga tttagtatat
     7381 acattttgct taagtttagc cacagaaata taagcctcga actcagtatt atatgcagcc
     7441 gaaccacata ttacaaaggg tgtttattgt ttttcttcac atttattcaa tatatacttt
     7501 tacaaatcat catttttgta ataataagaa tagtctcgaa aagtaactgg aaaacctgct
     7561 gaatgttaat taatgggata aa
//
LOCUS       DAOOJC010000058         7557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_8215_length_7557_cov_3.413756, whole genome shotgun sequence.
ACCESSION   DAOOJC010000058 DAOOJC010000000
VERSION     DAOOJC010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8215_length_7557_cov_3.413756"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /gene="nusA"
                     /locus_tag="PLA01_02720"
     CDS             <1..1084
                     /gene="nusA"
                     /locus_tag="PLA01_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPD00245.1"
                     /translation="SSQNVKVFIDRQTGDFKVFALKKVTSSPQNDLLEISIENAKKIN
                     PELEEDDVVEIEVTPRKFGRIAAQTAKQVVVQRLREAERGIIFNEFSNKEGEIVTGIV
                     QRTERRNVIIDLGKTEAVLPQTEQIPGEEYKFNDRIKTYIIEVKKTTKGPQIMVSRTH
                     PGIVKRLFELEVPEIYEGIVEIRSISREPGSRTKIAVFSKDDNVDPVGACVGQKGTRV
                     QAIVDELRGEKIDIIKWSSNPQEYISSSLSPAKVVRVDINEEEKSAKVTVPDFQLSLA
                     IGKEGQNARLAAKLTGWKIDIKSESQLRATIEQQLLNFDGSYNALNDEEESFEYNQMD
                     NYQNDEEVEDTQEQEEKDFFDESDEN"
     gene            1173..1445
                     /locus_tag="PLA01_02725"
     CDS             1173..1445
                     /locus_tag="PLA01_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HPD00246.1"
                     /translation="MKQKKIPMRMCLGCQEMKPKKELIRVVKNKENEISIDFTGKKPG
                     RGAYICRSLSCFEKARKGKRFERAFEVSINEEIYEMLKKDLEDGNV"
     gene            1438..1788
                     /locus_tag="PLA01_02730"
     CDS             1438..1788
                     /locus_tag="PLA01_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HPD00247.1"
                     /translation="MSERIYSFLGLARKANKLVSGEETCERAIKAGRVSLVIVAEDAS
                     ANTKKKFTDACSYNNINIRIFGKKELLGKFIGKDIRSVIAIQDLGFSERIMQMIDEEK
                     IGCGGESIDKKKSI"
     gene            1766..4966
                     /gene="infB"
                     /locus_tag="PLA01_02735"
     CDS             1766..4966
                     /gene="infB"
                     /locus_tag="PLA01_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPD00248.1"
                     /translation="MTKKRVYEIAKELNTTSKRLMEKLAEIKIEVKNHMSLLEEDQLE
                     ALYDHIGVIRHNEEKSDIDDKKTTSAPPVVQQNVEKKKEDKKAPRIIRTTEIYLDSQE
                     AEKENEPAGQKNNKKDGKRRRNDFIKVATSTSGLRPGFVRDTKPDFKKLVENDKKELA
                     AKENNKPVDKGKIMENEKIAKTAEIQEITTPVEKKVESKPVVDNKDNSPAGENKELKS
                     GNKTEAIKDASSKNDGITTPAHTGALDAQVKPDTVNVPANAQTKKDKEGKDIVNAQKP
                     VNVRNNPPTANRNDDNGKSDERKRDFKTNDNKKGNINNDFKRNDKRDGSFKKDGNFRN
                     KDNKNDFNRPKDGKPGFRDKTAPFGDTGRDINRTANKRLEIPKVELTPTQKEELNSQR
                     AERRELNKDADKDTKREFKKEVAKPGIHAVKNKSSKVLKSVLTSKKGVSDVLSNDFEL
                     DDLFIDGVKVKKKTKKAKKEEKKAEKHIPPKAVLTSIKIPEVLTVKELAESLKKTSAE
                     VIKKLMSLGILATLNQELDFDTAAIVSDEFGVKAEKEVVINEEDILFDDNDDANDPQA
                     VPRPPVVVVMGHVDHGKTSLLDAIKQTNVIESEAGGITQHIGAYMVKINNRNITFLDT
                     PGHEAFTAMRARGAQVTDIAILVVAADDGVMPQTIEAINHAKAANVTIIVAINKIDKP
                     AANPDRVKQELTEYGLVPEEWGGDTICVEVSAKQRINIDNLLEMVLLVADMTELKANP
                     NKQAKGTVIEARLDKDKGPIATLLVQRGTLNTGDSIITGVTVGRIRAMTDDKGRKIKK
                     AGPSTPVEILGLPEVPEAGEIFHAITDEKVAKHLVEKRKLKQREQTLKATARVSLDDL
                     FNQIKEGKVKDLNIIVKADVQGSVEAVKQSLEKLANDEVRIKIIHGGVGSVTESDVTL
                     AQVSNAIIIGFNVRPSSNVVDAAKDAGVDLRLYRIIYDAIEDIQSAMKGMLDPTYREV
                     VLGHIEIRQTFKVSGIGTIGGGYVTDGKVVRNSDIRLVRDGIVIHEGKLASLKRFKDD
                     VKEVSQGFECGLSLERFNDIKEGDVVEAFSIEEVKR"
     gene            5024..5371
                     /gene="rbfA"
                     /locus_tag="PLA01_02740"
     CDS             5024..5371
                     /gene="rbfA"
                     /locus_tag="PLA01_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPD00249.1"
                     /translation="MDRIARISEEIKREISNIIQNELKDPRLSTLISITYVNVTKDLR
                     YAKVYVSILGNDEEKNSSLEGLRSAAGFIRREIGHRVQLRYTPEIHFELDNSIEKGAY
                     ITKLISETSNQDE"
     gene            5430..6386
                     /locus_tag="PLA01_02745"
     CDS             5430..6386
                     /locus_tag="PLA01_02745"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPD00250.1"
                     /translation="MIENRIISQINEANWIAVLPHVSADGDALGSSLALALALKAISK
                     TATVYIEEEIPSIYRFLPGKELIQVYNEKPDKYELVLALDTGDLDRLGKRVSIFREAD
                     RTVNIDHHTTNTEFAQLNLVKTNSSAVGEIIYQIIKMMGITIDRDMATCLYVAITTDT
                     GGFRYSNTTPITHQIAADLVNSGVNVADISQIVFETTSLPKIKLMGKVIENLELLENG
                     KLAFVHITDDMLKSTDAGEEDCDGLVNIGRNIEGVEVSVFMRQRGDDEYKINFRSKTY
                     VDVSRIASRHSGGGHVRAAGCTVKGNMNEIKSALIKEIREVL"
     gene            6389..7279
                     /gene="truB"
                     /locus_tag="PLA01_02750"
     CDS             6389..7279
                     /gene="truB"
                     /locus_tag="PLA01_02750"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPD00251.1"
                     /translation="MNGILNVLKPPGMTSFDVVAYLRGLLKVKKIGHTGTLDPGAVGV
                     LPVCIGKATKAIEYLMDKDKVYRAELTLGISTDTQDSLGRVLKEAEVNSSKDEIYAAV
                     KSFVGSYSQLPPMYSAIKKNGKKLYELARSGIEVERSPREVMIRFINILSIKENTVVF
                     DVGCSKGTYIRTLCSDIGDRLGCGGHMSFLVRMKAGSFTIDNALTVEQIKEMAQNGTI
                     EKAFIGIEEVFESFGRLYLNKEEEKRFLNGGFIRIGGLAPALPQKVCVFGENGKILAL
                     GETVFNKKGELSLKPLKIFV"
     gene            7364..>7557
                     /locus_tag="PLA01_02755"
     CDS             7364..>7557
                     /locus_tag="PLA01_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FMN
                     adenylyltransferase"
                     /protein_id="HPD00252.1"
                     /translation="MKVVYGTDECTKFIRPTGIGLGNFDGLHVGHLALINTLITESVF
                     NSFDSVLYTFSSHTENIINK"
BASE COUNT         2620 a         1132 c         1909 g         1896 t
ORIGIN      
        1 ttcatctcaa aacgtgaaag tttttattga ccgccagacc ggtgatttta aggtgtttgc
       61 attgaaaaag gttaccagca gtcctcaaaa tgatttgctg gaaatatcga ttgaaaacgc
      121 gaaaaaaatc aatccggagc ttgaagagga cgatgtggtt gaaatagagg ttacaccaag
      181 gaaatttgga agaatagctg ctcaaactgc taaacaggtg gtagttcaac gtctcaggga
      241 agctgaaaga ggaattattt ttaatgagtt ttccaataag gaaggcgaga ttgttaccgg
      301 aattgttcag aggacggaaa ggagaaacgt aatcattgac cttgggaaaa ccgaggcagt
      361 attaccgcag acggagcaaa taccggggga agaatataag tttaatgacc gaataaagac
      421 ttatataatt gaggtcaaga aaaccacaaa aggtcctcag attatggttt caaggacgca
      481 tcccggaatt gttaagaggc tctttgagct tgaagttccg gaaatttatg agggtatagt
      541 ggagatcagg agtatttcta gagaaccggg ttcaagaaca aaaattgcgg ttttctcaaa
      601 ggacgataat gtggatcccg tcggtgcatg tgtgggtcaa aagggtacga gggttcaggc
      661 tattgttgat gaacttaggg gtgaaaaaat agatattatt aagtggagca gcaaccccca
      721 ggaatatatt tccagcagtc ttagccctgc aaaggtggta agagtagata taaatgaaga
      781 agaaaagagt gcgaaggtga ctgtacctga ttttcagctg tctttggcaa taggcaagga
      841 aggccagaat gcaagacttg cagcaaagtt aaccggatgg aaaattgata taaagagcga
      901 atcgcagctt agggctacta ttgaacagca gctcttgaat tttgacggca gctacaatgc
      961 tttaaatgat gaagaagaaa gctttgagta taatcaaatg gataattatc aaaatgatga
     1021 agaagttgaa gatactcagg aacaagaaga gaaggatttc ttcgatgaat ctgatgaaaa
     1081 ctagatttgt gggctgaaca tcgaaaatga gagagattta ttgcattttc aagggtatat
     1141 cacagatagt attatttggg atgtgatgag cattgaaaca aaaaaaaatt ccgatgagaa
     1201 tgtgccttgg atgtcaggag atgaaaccta aaaaggaact cattagggtt gtgaagaaca
     1261 aagaaaacga aatctctatt gattttacgg gtaaaaagcc gggtagaggt gcatatatct
     1321 gcaggagcct ttcatgtttt gaaaaagcaa gaaagggtaa aaggtttgaa agggcattcg
     1381 aggtatctat caatgaagaa atatatgaaa tgttgaaaaa agatctggag gatggtaatg
     1441 tctgaaagaa tttactcttt cttggggtta gcaagaaaag caaataagct ggtctcaggt
     1501 gaggagacat gtgaaagggc tattaaggca ggaagggtaa gcttggtaat tgtggcggag
     1561 gatgcatcag ccaacacaaa aaaaaagttt acggatgcct gtagttataa taatataaat
     1621 attagaatat tcggtaagaa agagttactg ggcaaattta tcgggaagga tataaggtcg
     1681 gtgatagcta tacaggactt ggggttttca gagcgtataa tgcaaatgat tgacgaggaa
     1741 aaaattggat gcgggggtga atctattgac aaaaaaaaga gtatatgaga tagcaaaaga
     1801 gcttaataca acaagcaaaa ggctgatgga aaaattggcg gaaataaaaa tagaagttaa
     1861 aaaccatatg agtcttcttg aagaggatca actcgaagca ttatatgacc atattggtgt
     1921 tattagacat aatgaggaaa aatcagatat tgatgataaa aagactactt ccgctcctcc
     1981 ggtagttcag caaaatgttg aaaagaaaaa agaagacaaa aaggcaccga gaattataag
     2041 gaccacagag atttatcttg attcacagga agcagaaaaa gaaaacgagc ctgctggaca
     2101 aaagaataac aaaaaagatg gaaaacgcag acgaaatgat tttattaagg tggctacctc
     2161 tacttcaggt ttgaggcccg gttttgtaag agataccaaa ccggatttta aaaagttggt
     2221 tgaaaatgat aagaaagaat tagcggctaa agaaaacaat aagccggtag ataaaggtaa
     2281 gataatggag aatgaaaaaa ttgcgaaaac agcagaaatt caggaaataa ccactcctgt
     2341 agaaaaaaaa gtggaatcca aaccggttgt tgataacaag gacaatagcc cagccgggga
     2401 aaacaaagaa ttaaaaagcg gtaataaaac tgaagcaatc aaagatgctt catcaaagaa
     2461 tgatggcata actacaccgg ctcataccgg agctttggat gcacaggtga agcctgacac
     2521 tgtcaatgtg ccggctaatg ctcaaaccaa aaaggataaa gagggtaagg acattgttaa
     2581 cgctcaaaag ccagttaatg ttcggaacaa tcctccaact gccaatagaa atgatgataa
     2641 cggcaagagt gatgaaagaa agagagactt taagacaaat gataataaaa agggcaatat
     2701 aaataatgat ttcaagagga atgataaaag agacggaagt ttcaaaaaag acggcaattt
     2761 tagaaataaa gacaacaaga acgattttaa ccgtccaaag gatggtaaac ccggattcag
     2821 agataaaacg gctcctttcg gtgacacggg tcgtgatata aaccgtacag caaataaacg
     2881 tctggagatt cccaaggttg agcttacacc gacccaaaag gaggaattga attcgcagcg
     2941 ggctgaaaga cgtgaattga acaaggatgc tgataaagat acaaagaggg aatttaagaa
     3001 agaagtggcc aagccgggaa tacatgctgt taagaacaaa agcagcaagg ttctaaagag
     3061 cgttctgaca tcaaagaagg gagtatcgga tgtattgtca aatgactttg aattggatga
     3121 tctctttatt gatggtgtaa aggtcaagaa gaagacaaaa aaggcaaaga aggaagagaa
     3181 aaaagcagaa aaacatattc ctccgaaggc tgtgcttact tcaataaaaa ttcctgaagt
     3241 tctgacggtt aaggaactgg ctgaatcact taaaaagact tctgcggaag tgatcaaaaa
     3301 gttaatgtcc ttaggaattt tggcaacact aaaccaagag ctcgattttg atacggcggc
     3361 aatagtatct gacgaattcg gtgttaaagc ggagaaagaa gttgttatca atgaggaaga
     3421 cattcttttt gatgacaatg atgatgcaaa tgatccccaa gcagtaccaa gaccgccggt
     3481 agtggttgta atgggacatg ttgaccacgg taagacatcc cttttggatg cgattaagca
     3541 gacaaatgtt attgaaagtg aagccggtgg aattacgcag cacatcggtg cttacatggt
     3601 aaaaataaac aataggaata tcacattcct tgataccccg ggacatgaag cttttacagc
     3661 tatgagagcc aggggagcac aggttaccga tattgcaata cttgttgtgg ctgccgatga
     3721 cggtgttatg cctcagacca tagaagcaat aaaccatgca aaggctgcaa atgtaacaat
     3781 tatagttgca attaataaaa ttgataaacc ggcagctaat ccggacaggg ttaaacagga
     3841 attgacagag tatggtcttg ttccggagga atggggcggc gatactattt gtgtagaggt
     3901 ttctgcaaag caaagaatca acatagacaa tctactcgaa atggttcttc ttgttgcaga
     3961 catgaccgag cttaaggcta atcccaataa acaggccaag ggaactgtaa tagaagcaag
     4021 gcttgacaag gataaagggc ctatagctac cttgctggta cagaggggaa ctttgaatac
     4081 aggtgattcc ataattactg gtgttactgt cggcagaatc agggctatga ctgatgataa
     4141 gggacgcaaa attaaaaaag caggtccgtc cactccggtg gagattcttg gtttgcccga
     4201 agtacctgaa gcgggggaaa ttttccacgc aattaccgac gaaaaagtag caaaacacct
     4261 tgtggaaaag agaaagctta agcaaagaga gcagactttg aaagcgactg ccagggtctc
     4321 tctggatgat ttgtttaatc agattaaaga aggaaaggtt aaggatctca atataattgt
     4381 taaggctgat gttcaaggtt ctgttgaggc tgttaaacag tctctggaga agcttgctaa
     4441 tgatgaagtc agaataaaga tcatacatgg cggtgtgggt tcagtaacgg aatccgacgt
     4501 aacgctggca caggtatcta acgctataat aatcggtttc aatgtaaggc cttcttcaaa
     4561 tgtagtagat gcagctaaag atgcgggtgt tgatttgaga ctttacagaa ttatatacga
     4621 tgcaatagaa gatatacagt ctgccatgaa aggaatgctt gacccgactt atagggaggt
     4681 tgtgcttggt catattgaaa tcaggcaaac attcaaggtt tccggaatag gtacaatcgg
     4741 tggaggctat gtcactgacg gaaaagttgt caggaattct gatataaggc tggtaaggga
     4801 cggaattgtt attcatgaag gcaagctcgc atcattaaag cggtttaagg atgatgtcaa
     4861 ggaggtttcc cagggctttg aatgcggatt atctttagag aggtttaatg acattaaaga
     4921 gggagacgtt gttgaggcgt tttctattga agaagtaaag cgataaaata ataattgcag
     4981 aacattttaa ctaaattgtt atgcagtatt aggagtgttt attatggaca gaattgcacg
     5041 tatttctgag gagataaaaa gggaaatcag caatataata caaaatgaat tgaaggatcc
     5101 aaggctttcc acgcttataa gcataactta tgttaatgtt acgaaggatc ttcgttatgc
     5161 aaaggtttat gtcagcattt tgggtaatga cgaggagaaa aacagttctc tggaaggcct
     5221 tagaagtgcc gccggtttta tccggcggga aatcggtcat agggttcagt tgaggtatac
     5281 gccggagatt cattttgagc ttgataattc cattgagaag ggagcttata ttacaaagct
     5341 tatcagtgaa acatcaaatc aggatgagtg agtattatta atatgcgaaa aagactgtgt
     5401 tctttatata gcttattgag aggtaggaaa tgattgaaaa caggattata tcacaaataa
     5461 acgaagcaaa ttggattgct gttttgccgc atgtttcagc ggacggtgat gcactgggtt
     5521 caagtctggc gttagctctg gcactgaaag caataagcaa gacggcgacc gtttatatcg
     5581 aagaggaaat accttcgatc tatagatttt tgcccgggaa ggagttaatt caggtttata
     5641 atgaaaagcc cgataagtat gaacttgtgc tggccctgga cacgggagat ttggatagac
     5701 tgggaaaaag agtctcgatt ttcagggagg ccgacaggac tgtaaatatt gaccatcaca
     5761 ctacaaatac tgagtttgca cagcttaatc ttgtaaagac aaattcctcg gcagtagggg
     5821 aaataatata tcagattatt aaaatgatgg gtataactat tgacagagac atggcaacat
     5881 gtctctatgt tgcaataacc accgataccg gagggttcag atacagcaat actactccta
     5941 ttactcatca gattgcggca gatcttgtca atagcggcgt gaatgtggcc gatatatccc
     6001 aaatagtgtt cgaaaccaca tcgcttccaa agataaagct gatgggtaag gttatagaga
     6061 atttggaatt gcttgaaaac ggaaaactgg cttttgtcca tatcaccgat gacatgctga
     6121 agagtactga cgcgggagaa gaggattgcg atggcctggt gaatatagga agaaatattg
     6181 aaggtgtaga agtatcggta ttcatgcgac agcgtggaga tgatgaatat aagattaact
     6241 tcaggtcaaa gacttatgtt gatgtctccc gcatagcaag caggcattcc ggcggcggac
     6301 atgtgagggc ggccggatgt actgtcaagg ggaatatgaa tgaaatcaag agtgcattga
     6361 taaaggagat cagagaagta ttatagctat gaacggaatt ttgaatgtac taaagccccc
     6421 ggggatgact tcctttgatg ttgtggcata tttgagggga ttattgaaag taaaaaagat
     6481 tggccatacc ggaacattgg atcccggggc ggtgggagtt ttgcctgttt gcatcggcaa
     6541 agccacaaag gctattgaat acctgatgga caaggacaag gtatacaggg cggagcttac
     6601 cctgggtata tccaccgaca cccaggacag tttgggaagg gttttgaaag aggcagaggt
     6661 gaattcttca aaggatgaaa tatatgctgc ggttaagtcc tttgttggca gctatagcca
     6721 gttgcctccg atgtattcgg caataaagaa aaacggaaag aaactttatg agctcgccag
     6781 aagcggtatt gaagtggaac ggagcccaag agaggttatg atccgtttta ttaacatatt
     6841 gagcattaag gagaacacag ttgtttttga tgtggggtgt tctaaaggga cttatataag
     6901 gaccttatgc tcggatatag gggataggct cggttgcgga ggacatatgt cctttctggt
     6961 cagaatgaag gccggttcct ttacgataga taatgcatta accgtagagc aaattaaaga
     7021 gatggcacaa aacggtacaa tagagaaggc atttattggc atcgaagagg tatttgagag
     7081 ctttggaagg ctatatctta ataaagagga agagaagagg tttttaaacg gaggctttat
     7141 taggatagga ggtttggcac cggctttacc gcagaaggtt tgtgtgtttg gagaaaatgg
     7201 gaaaattttg gctctgggag agacggtttt taataagaaa ggagagctgt ctctcaagcc
     7261 gttgaagata tttgtatagg gttgtgtgaa gaaaaatatt ccagaaactt aagttccgca
     7321 gtttagagac tgtaaataat agaaatattt aaagggaagt aatatgaaag tagtatatgg
     7381 gacggatgaa tgtacaaaat ttataagacc aacgggtata gggcttggta attttgacgg
     7441 tttgcatgtg ggtcacctgg ctctgattaa cacgcttatt accgaatctg tttttaactc
     7501 ttttgattcg gtgttgtata cctttagtag ccatactgag aatattatta ataaaaa
//
LOCUS       DAOOJC010000059         7538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_8238_length_7538_cov_3.525324, whole genome shotgun sequence.
ACCESSION   DAOOJC010000059 DAOOJC010000000
VERSION     DAOOJC010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8238_length_7538_cov_3.525324"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /gene="cobS"
                     /locus_tag="PLA01_02760"
     CDS             complement(<1..631)
                     /gene="cobS"
                     /locus_tag="PLA01_02760"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HPD00253.1"
                     /translation="MKYIKRFILMIQFLTTIPIPCRIDCDEEDCGKGLIFAPVIGLLL
                     GGILTAFFYLLQLFFSLSVVSAFMLVIYILLTGGLHLDGLGDTFDGLFSNRPRERMLE
                     IMRDSRIGTNALLGVVCIILLNYSLLNAMPVSSLPWVLLLFPVAGRAGTLIGAGLSVY
                     AREGEGLGKSFVHYCTIKEVLTGMLIYFLFFFLVLGIGGLLMAFLTSLTA"
     gene            complement(666..1226)
                     /gene="cobU"
                     /locus_tag="PLA01_02765"
     CDS             complement(666..1226)
                     /gene="cobU"
                     /locus_tag="PLA01_02765"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HPD00254.1"
                     /translation="MKKFILVTGGARSGKSSFAQSLAHNAGDEQVLYIATSIPFDDEM
                     RARVKKHREQRPGGWITLEAYKDFDMQLGKVHEKKKVILLDCITLMVSNIIMEKCTDW
                     SILPEGFEEKIEQDVMLEIDKLLSAVRGLDTTFIAVTNETGMGIVPENKLSRLFQDVA
                     GRVNQVLARNADDVYLCVSGIPVKIK"
     gene            complement(1287..2342)
                     /gene="cbiB"
                     /locus_tag="PLA01_02770"
     CDS             complement(1287..2342)
                     /gene="cbiB"
                     /locus_tag="PLA01_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HPD00255.1"
                     /translation="MDLYLLLDLYIAYVIEILIGFPRFLPHPVKFIRWLIKIVEKMVR
                     AMIRVSSEKKVKALGDDVVRNTRKAYRNERIAGTLLALLVIAVVVSIVFGILKACMFV
                     HPLVFHIVNTIFIYVSFASRSVAFGAFTVFDALKERDLFKARKMVTEFIGIKTEDLDE
                     SGMIKGTVEKVAKSTSDKVVAPVFYATIGSLLGIGAPMVFAYMTISILDSMVGHKNSE
                     YKNIGFIPAKLDDIVNFIPARLTGLLVVVGAMLTGKNYKKGYSVMMRDRRKHKSPNFG
                     YPEAAIAGVLGIRLGGPGLYFADIVDKPVIGDDDNPLDIKNITQTITIMYVTSIIAMV
                     GLGAVAAAVVVLCNRFI"
     gene            complement(2905..4251)
                     /locus_tag="PLA01_02775"
     CDS             complement(2905..4251)
                     /locus_tag="PLA01_02775"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPD00256.1"
                     /translation="MKVVKFGGSSLADAHQIKKVCDIILSDSDRRLVVVSAPGKRSKE
                     DTKVTDLLIALGQKYLKEGKADDELIAVIDRYDEIVKGLGLSSDVSDTVANDLKSRLN
                     GDVSNEERFMDAIKAAGEDNNAKVIAAYLVSKGIDAAYVNPQDAGLLLSDEYGNARVL
                     PESYDNLKALKDRKELLIFPGFFGYSKSGSVVTFPRGGSDITGSILAAAVKADVYENF
                     TDVDSVYAANPNIIDNPKPIPAFTYREMRELSYSGFSVLHEETLEPVYRMGIPVCIKN
                     TNNPSAPGTMIAPTRKLNNGPVVGIASDTGFCNIFVGKYMMNREIGFGRKVLSILEDE
                     GLSYEHIPSGIDNMSIVLRQKQLDEVKEKRIINRIKEELKADDVTVERNRALVMIVGE
                     GMMRSVGILSRASAALAKANVNVEMINQGSSEVSMMFGIKEEDKVVAVKALYNEFF"
     gene            complement(4369..5619)
                     /locus_tag="PLA01_02780"
     CDS             complement(4369..5619)
                     /locus_tag="PLA01_02780"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPD00257.1"
                     /translation="MINVAVLGYGIVGSGVVEIIKKNGISISEKAGQKICVKKILDIR
                     DFPDSPDRELITKNSDEIMEDSSIGIIVETIGGTGIAYKLTKDALSRGKHVVTSNKEL
                     VATHGPELLKLAKENNSNYLFEASVGGGIPIIRPLNRCLAANEIYSITGILNGTTNYI
                     LSQMKREGKNFDEALKEAQARGYAEADPTADIEGHDACRKIAILSSIAYNEFVDCNNI
                     YTEGISKISLADMKYAESMNSTVKLIATSEKVGDKIAARVSPAFVKSSNPLYNVENVF
                     NAIIVKGDAIGDVMFYGRGAGKLPTASAVVADVIEIVKHWGAAGGYDWEVKNQDNIIN
                     INDTETRYFVRLKVKDESYAKEAVEKVFGNVEWVKIYGDGPKDELAFVTGKVPEKEHI
                     RAFKNIEGHDSVIEVSNTIRVLGD"
     gene            complement(5635..6078)
                     /locus_tag="PLA01_02785"
     CDS             complement(5635..6078)
                     /locus_tag="PLA01_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPD00258.1"
                     /translation="MNNKSKYYLVDSSVLPEVFHKVVEAKKLLSSGAIKNINEAVQRV
                     GISRSAYYKYRDYIFPFYETTRGKVVTLFFTVEDYSGILSSIINKIAESKANIITINQ
                     NIPINGLADVTITIETDSMINDIKDLMFEISNIEGVRKQQILARE"
     gene            complement(6275..>7538)
                     /locus_tag="PLA01_02790"
     CDS             complement(6275..>7538)
                     /locus_tag="PLA01_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HPD00259.1"
                     /translation="GYKDLAPPLKTIKTLDIQGYCEKTDNFDQLILLIESAIKTISQK
                     KTIKVFKDGLKRILDSVPKIYQLQSISEILENILGEVMFFVKCSNAFIMVDNFSLEGN
                     WDESAIKGIGTYDVSLEKFMKILEPSFMESIGHARASKECIRTDEGIILPLMDDSMKT
                     LGVIYLEFDHPFNEIELLKIFATQVATSINNIHLHSLISIKNDELDRTYNELQKRYMD
                     AIQVLRLAVDAKDMYTRGHSDRVAYYAVKIGKSFNLKEEELQKLKAAGIFHDIGKIGI
                     ADDILFKTNKLNDIEYDEIKKHPIKGAHILSAVSMFKDIVPLVRCHHERTDGRGYPYG
                     IKGEEIPFLARIISVADAFDAMTSDRKYRSKLDIEEAKKQLILNSGTQFDSNVVNNFI
                     TLLNDYDEMQKEIEYTFNPNRDSGKEKE"
BASE COUNT         2247 a         1807 c         1120 g         2364 t
ORIGIN      
        1 ttgcagtcaa ggaggtaaga aatgccatca gcaagcctcc aatccccaac accaaaaaga
       61 aaaaaagaaa gtaaattagc attcctgtca atacttcttt tattgtgcaa tagtgtacaa
      121 aagacttacc caaaccttca ccctctctgg cataaaccga taatcccgca ccgattaatg
      181 tacctgcccg cccggcaaca ggaaacagca acagtaccca cggaagagaa gataccggca
      241 ttgcattcag cagagaataa tttaaaagaa tgatacacac aacacccagc aatgcattgg
      301 ttccaatcct gctgtcacgc attatttcaa gcattctttc tctcggtcgg tttgaaaaca
      361 atccgtcaaa ggtatcccca agcccatcca ggtgaagtcc gccggtcaat aatatatata
      421 ttaccagcat gaaagcactt acaacactca aagagaaaaa aagctgcaat aaataaaaaa
      481 aagctgtcag tattcctccc aacaacagtc ctataacagg agcaaaaatt aagccttttc
      541 cgcaatcctc ttcatcacag tctatcctac agggtatggg aattgtagtc aagaactgta
      601 tcataagtat aaacctcttt atatacttca aacctatcac tcccaaaaac aagctatatt
      661 cattcttact tgattttaac aggaatgccc gatacacaca ggtacacatc gtcggcattt
      721 cttgcaagca cctggtttac ccttccggca acatcctgaa aaagtcttga aagcttattt
      781 tcaggcacga ttcccattcc cgtctcattt gtcacagcaa tgaacgtagt gtcgagccct
      841 cttacagcag aaagcagctt atcaatttcc agcattacat cctgttcgat tttctcttcg
      901 aagccttcag gcagtatact ccaatcagtg cacttttcca taataatatt ggaaaccata
      961 agggttatgc aatcaagcaa aatgaccttc tttttttcat gtaccttgcc aagttgcatg
     1021 tcgaagtcct tatacgcttc gagcgttatc cagccacccg gcctttgctc cctgtgcttt
     1081 tttacccttg ctctcatttc atcatcaaag ggtattgatg tggcaatata cagaacctgt
     1141 tcatctcctg cgttatgtgc taaagactgt gcaaatgaac ttttaccgct tcttgctcct
     1201 cccgtaacga ggataaactt cttcattgct tcaaacacct cagtataata tctatcatac
     1261 aataaaaata catcataaaa atcccgctaa ataaagcgat tacaaagaac aaccaccgct
     1321 gctgccaccg cacctaagcc caccatggca ataatagatg taacatacat tattgtaata
     1381 gtttgagtaa tattttttat atccagagga ttatcatcgt ctccaattac aggtttatcc
     1441 acaatatcgg caaaatacag tcccggccct ccaagcctta ttcccaacac tcccgcaatc
     1501 gcagcctcag ggtagccgaa attcgggctt ttgtgctttc ttctgtctct catcatcaca
     1561 gaataaccct ttttataatt ttttcccgta agcattgctc caacaacaac taacagccct
     1621 gtaagccttg caggaatgaa atttactata tcatcgagct ttgcgggtat aaatcctata
     1681 tttttatact cactgttttt atgtcccacc attgaatcca aaatactaat ggtcatataa
     1741 gcaaaaacca ttggtgctcc aatacccaat aaagagccaa ttgttgcata gaaaaccgga
     1801 gctaccactt tatccgaggt gctcttggcc actttttcaa ctgttccttt tatcattcca
     1861 ctctcatcaa ggtcttcggt cttaattcca atgaattcgg tcaccatctt tcttgcctta
     1921 aacaagtccc tttccttgag tgcatcaaaa acagtaaagg ctccaaaggc caccgatctt
     1981 gaggcaaaag aaacatatat aaatatagta tttacaatgt gaaatactaa aggatggaca
     2041 aacatacaag cctttaaaat accgaaaaca atcgacacta ccacggctat taccaacaat
     2101 gctaaaagag ttccggctat cctctcattt ctataagcct ttctggtgtt tcgaaccacg
     2161 tcatccccca atgccttaac ctttttttcc gacgaaaccc taatcatagc cctaaccatt
     2221 ttctccacaa tttttatcaa ccagcgaata aactttacag gatgcggcaa gaagcgtggg
     2281 aaacctatta atatttcaat aacataagct atgtataaat caagcagtaa atacagatcc
     2341 attgcccttc ccccttacac tattcattat tacaaaactc ctaaaacact actgaacctg
     2401 caatactgca agcaaatcaa tgatttaatg tagtaccgac tgtaaaattc gcaacggttg
     2461 aattgggaat atatcaattt cgtgatgctg caatggtaat ataaagcaat tttgaatttc
     2521 aagaaaataa tccactgtat cccaaatcac agaaatagta ggaaattata ttataataag
     2581 gtgtatctat aattattata tcgtctttat ttataagttc aacatacata ttcatggtga
     2641 acaattgtat tatagcacat tattcatgat ttgcaacaat cctttttaaa tacgatacta
     2701 tttccgggat gtaaaaatta acaaaagctc tgtaagcatc aagccactac cgctttttgt
     2761 ttattcctct ttgtaaaatc ctaccaaatt aacgtgaaga ataatacttt agaaacttac
     2821 gtttcttaca tattattctt cacgtaaaca atatatgacc tgtattgaat acttaaaaga
     2881 aatattaagg attaatatca acaactaaaa aaactcgtta taaagtgctt ttacagctac
     2941 aaccttatcc tcttccttaa tcccgaacat catactaacc tccgaagagc cctgattaat
     3001 catttcaaca ttgacattgg cttttgccag tgctgcggaa gccctcgaca aaatacccac
     3061 acttctcatc ataccctccc cgacaatcat aacaagggca cgattgcgtt caacagtaac
     3121 atcatcagcc ttcaattctt cctttattct attaataata cgcttttcct taacctcatc
     3181 aagctgtttt tgccgaagta caatagacat attgtcaatt cctgagggaa tatgctcgta
     3241 agacaaaccc tcgtcctcaa gaatgcttaa aacttttctt ccgaatccaa tctccctgtt
     3301 catcatgtac ttgccgacaa aaatgttgca aaaccctgtg tcactggcaa ttcctacaac
     3361 aggaccattg ttcagtttcc tcgtcggggc aatcattgtt cccggtgccg acggattatt
     3421 ggtgttcttg atgcataccg gaattcccat tctatagacc ggttcaaggg tttcttcatg
     3481 caatacggaa aaaccggaat aggaaagctc cctcatttcc ctataggtaa aagccggaat
     3541 aggctttgga ttatcgataa tattcggatt agcagcataa acagagtcaa cgtcggtaaa
     3601 attctcatat acatcagcct ttacagctgc agccaatata gatcctgtta tatccgaacc
     3661 gccccgggga aatgtcacaa cactcccgga ttttgaatat ccaaaaaagc cggggaaaat
     3721 caacagttct tttctgtcct tcagggcttt taaattatca taggattccg gcagcacacg
     3781 ggcgtttcca tattcatcac tgagcaaaag accggcatct tgtggattta catatgccgc
     3841 atctattcct ttacttacaa gatacgcagc aattactttg gcattattgt cttctcccgc
     3901 tgccttaatg gcatccataa acctttcctc gttactcaca tccccgttaa gacggctttt
     3961 caggtcattg gcaactgtat ccgacacatc ggaggatagc ccaagtcctt ttacaatctc
     4021 gtcatatcta tcgataaccg caattaattc atcatctgct tttccttcct taaggtactt
     4081 ttgtcccaac gcaattaaca ggtccgttac cttcgtgtct tctttcgaac gtttgcctgg
     4141 agcagaaacc acaaccaagc gtctatcact gtcacttaaa attatatcgc atactttttt
     4201 tatctgatgt gcatcagcca atgatgaacc tccaaatttt acaaccttca taaaattacc
     4261 tcctaaacgc ttttatgtaa tactataatc atgctcatta agcactctta ttaacctaat
     4321 tgtataaata ccaaaacgca cctttcataa tgcaaatttc ctatgggttt aatctcccag
     4381 caccctgata gtgtttgaca cctctattac agaatcatgc ccctcaatat tcttaaaagc
     4441 cctgatatgc tctttctccg gtacttttcc ggtaacaaaa gccaattcgt ccttcggacc
     4501 gtcaccataa atctttaccc attcaacatt tccaaaaact ttttccacgg cctcttttgc
     4561 ataagactca tctttcacct tcaacctgac aaaatacctt gtttcagtat cgttaatatt
     4621 aataatgtta tcctggttct tgacttccca atcatagcct ccggctgctc cccaatgctt
     4681 gacaatttcg atcacatccg ccacaacagc actggccgtg ggaagtttac cggctccgcg
     4741 gccataaaac atcacatcac caatcgcatc gccctttaca ataattgcat taaacacatt
     4801 ctcaacatta taaaggggat tgctgctttt cacaaaagcc gggctgactc gtgctgcaat
     4861 tttgtctcca accttttcac tggttgcaat aagtttaacg gttgaattca tgctttctgc
     4921 gtatttcata tctgcaaggc ttattttaga tatgccctcc gtataaatat tattgcaatc
     4981 aacaaactca ttgtatgcaa tcgaagagag aattgctatt tttctgcatg catcatgccc
     5041 ttcaatatca gcagtgggat cagcctccgc atatcctctg gcttgagcct cttttaaagc
     5101 ctcatcaaag ttctttcctt ctcttttcat ctgacttaag atatagtttg ttgttccgtt
     5161 aagtatgcct gttatggaat aaatctcatt tgcagcaagg caacggttca acggcctgat
     5221 aatcgggatt cccccaccaa cacttgcctc aaacaaatag tttgaattgt tctcttttgc
     5281 aagctttaaa agctctggtc catgagttgc aaccagttcc ttgtttgatg tcacaacatg
     5341 ctttcccctt gagagggcat ctttggttaa tttataagca atacctgtcc ctccgatagt
     5401 ctcaacaatt atcccaatgg aagaatcctc cataatctcg tccgaattct ttgtaataag
     5461 ttcacgatcc ggactatccg gaaaatccct tatgtccagt attttcttca cacaaatctt
     5521 ttgacctgct ttttctgaaa tacttattcc attctttttt ataatctcca caacccctga
     5581 gccaactatt ccatagccca gtactgctac atttatcatt attccaaacc tcctctattc
     5641 tctggcaaga atttgctgtt ttctgacacc ttctatattg cttatttcaa acattaggtc
     5701 ctttatatcg tttatcattg aatcggtctc aatggtaata gtaacatctg caagaccgtt
     5761 tatgggtatg ttctggttta tggttattat gtttgctttt gattccgcaa tcttatttat
     5821 aatactcgat aaaatacccg agtaatcctc tactgtaaag aaaagcgtta caaccttacc
     5881 ccgcgtagtc tcataaaacg ggaaaatata atccctgtat ttataataag cacttcggct
     5941 tatacctact ctctggacag cctcgtttat atttttaatg gctccgcttg ataaaagctt
     6001 tttcgcttca accaccttgt gaaaaacctc tggaagaacc gaagaatcca ctaaatagta
     6061 ttttgattta ttgttcatgc tctccctccc aacctgtcca tctgtcgtgt acaaatgtgt
     6121 ttctattgtg taatagtaac ataaaaacgc tgcagattca agatgtaaaa taaaaaaagt
     6181 ttcagttaac ataaatgtta aaaaattatc cctgaaactg aagcctcaca aaaaaagcac
     6241 tcctttaatg gaatgctttt atgttaactt attcttattc tttttctttc cctgaatccc
     6301 ggttgggatt aaaggtatat tcaatctcct tttgcatttc atcataatca ttcaacagag
     6361 ttataaaatt attcaccaca tttgaatcaa actgggtacc tgagtttagt ataagctgtt
     6421 tcttagcttc ctcaatgtcc agcttggatc tgtatttccg gtcagatgtc atggcatcaa
     6481 aagcatccgc caccgaaatg attcttgcca gaaacggaat ctcttctcct tttatcccat
     6541 aaggatatcc acggccgtct gtcctctcgt ggtggcacct taccaaaggt acaatatctt
     6601 taaacattga gactgccgaa agtatatgtg caccctttat aggatgcttt tttatctcat
     6661 catactcaat atcgtttagc ttatttgtct taaacaaaat atcatcagca attccgatct
     6721 tccctatgtc atggaagata ccggctgcct taagcttctg taattcctct tccttaagat
     6781 taaagctctt gcctatcttc acagcataat acgcaaccct atcggaatgt cctcttgtat
     6841 acatatcctt tgcatcaact gccagacgca acacctgtat ggcatccata tatctcttct
     6901 ggagctcatt atatgtcctg tccagttcat cgttttttat actgataagg gaatggaggt
     6961 gtatgttgtt tattgatgtt gccacctggg ttgcaaatat cttcagaagc tcaatctcgt
     7021 taaaaggatg atcaaactca aggtatatta ctccaagagt tttcattgaa tcgtccataa
     7081 ggggaaggat aattccttca tcagtcctga tgcattcttt tgaagcccgt gcatgtccta
     7141 tactttccat aaatgacggt tcgagtattt tcataaattt ttccaggctg acatcatatg
     7201 ttcctattcc cttaatagca ctttcatccc agtttccttc aagactgaag ttatcaacca
     7261 taataaacgc atttgagcac ttaacaaaaa acattacctc tccgagaata ttctcaagta
     7321 tttcggatat agactgcaat tgatagattt taggcacaga atcgagtatt ctcttcaatc
     7381 cgtccttaaa aacctttatt gtttttttct gggaaattgt ttttattgcc gactcaatca
     7441 gaagaataag ctggtcaaaa ttgtccgttt tctcacaata tccctgtata tctaaggttt
     7501 taatggtttt taacggaggt gcaaggtctt tatatcct
//
LOCUS       DAOOJC010000060         7521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_8261_length_7521_cov_3.496518, whole genome shotgun sequence.
ACCESSION   DAOOJC010000060 DAOOJC010000000
VERSION     DAOOJC010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7521
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8261_length_7521_cov_3.496518"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PLA01_02795"
     CDS             <1..648
                     /locus_tag="PLA01_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HPD00260.1"
                     /translation="DRVFGDKLQTVLIYISVVISILFSVGLFMVLPNAIAWLLPIDET
                     TTIGSILYNIVEGIIRVALFILYLSLTSQLKDIKRVWQYHGSEHKTIHCYENEEELTV
                     ENVKKYSTRHPRCGTSFLFTIMIVSIMVFSLVGQHGLWINILLRIVLIPLVAGVSYEI
                     IKIAGKSPSKIARIVNAPGLMFQGFTTREPDDSQIEVAIEAMKNVLVENKEEDKW"
     gene            661..1557
                     /gene="prmC"
                     /locus_tag="PLA01_02800"
     CDS             661..1557
                     /gene="prmC"
                     /locus_tag="PLA01_02800"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPD00261.1"
                     /translation="MILKDALLKGTQQLKMADIDAPAFEAGAILCYILKVDRSFLYSH
                     DDYKMTDEEYRCFDAFIGERAKGKPLQYITGHQEFMGFDFVVSPDVLIPRQDTETLVE
                     TVLNHVKDSGNKKAMILDIGTGSGCIAISLAAYIENSRVVACDVSEKALDVARTNAQR
                     CGVENRVDFICGDILQGLDKALCQSTFIKDKKEHNFEAFDVIVSNPPYIPAWEIQTLH
                     KQVKDHEPGLALNGGEDGLDFYRKITAEAVRFIKPHSLLAFEVGYNQAKDVSKLMEDG
                     YRDIRTVKDLSGIDRVVTGLRK"
     gene            2293..3615
                     /gene="rsxC"
                     /locus_tag="PLA01_02805"
     CDS             2293..3615
                     /gene="rsxC"
                     /locus_tag="PLA01_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPD00262.1"
                     /translation="MFVKRFFKGGTNVPHYKNTANCETVKMGVPEKVIIPMIQHIGAP
                     CEPCVQKGDTVKVGQIIGDSGKFISAPVHSSVSGTVVGVAPMLQSGGFYVPAVEIETD
                     GKQEVYEGIKPPKYSNIKEFIEAIRQSGLVGLGGAGFPAHVKLSPPPDKKIDTLVING
                     AECEPFITSDYREIIENSWNVISGINIVMEILNINNVIIGIEDNKPEAIEVMERIDKS
                     SDKISVVKLKSRYPQGAEKMLIYATTRRKVPAGKLPADVGVIVMNVNSVSFISEYVKT
                     GMPLIKKRVTVDGPSVKNPGNVEVLIGTPIHKVFDFCGGFTVPPKKVLMGGPMMGLAQ
                     YSLDLSVVKHTNALLAFDDKSSKIPESGACIRCGRCVWACPMNLMPLCLYNNAIKENI
                     DELNKYHVNDCIECGCCSYVCPAKINLVQSLRLGKAKVKQAAAAKGGK"
     gene            3617..4600
                     /locus_tag="PLA01_02810"
     CDS             3617..4600
                     /locus_tag="PLA01_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPD00263.1"
                     /translation="MEDRLTVSSSPHIRDKANTQRIMIDVVIALLPATFAGVYFFGTR
                     VLLVVMVSILSCVVSEYLSRRIMKRSNTISDFSAVVTGLLLALNLPPTVPLWVAAVGS
                     AIAIVVIKQLFGGLGQNFVNPALGARIILMISYPGFMTNWVKPGVDAVSAAASGVDAI
                     SSATPLQIMKYGASIADLPSYGELFWGNIMGCLGETSAAALLIGAVYLFARKIISPEI
                     PFAFIGTTALFTWIFGGTTLFTGDFLFHVLAGGLLLGAIYMATDYATSPMTVKGRIIM
                     GIGCGLLTGIIRLFTNYTEGVSFAIVLMNIMVPLIDRYTIPKSFGGEKAVA"
     gene            4581..5186
                     /locus_tag="PLA01_02815"
     CDS             4581..5186
                     /locus_tag="PLA01_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HPD00264.1"
                     /translation="MKKPLRDIAKPTVVLFLICTIVTFALAYTNAITADKIDEMISLE
                     LDNARRKVLSLADSFEKIEGLEEIVSENADLGIVKEAYKGVKGQETVGNVFLVESKGY
                     GGAITITVGIDNKGKITGVEIGDNNETPGLGAKAKDEPFISQFVGIIPEEPLTVVKSN
                     KSKSEEIDAISSATITSRAVTKAVQAAVDINAELMKRGGNS"
     gene            5188..5862
                     /locus_tag="PLA01_02820"
     CDS             5188..5862
                     /locus_tag="PLA01_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HPD00265.1"
                     /translation="MSAVKEGFKNLTRGIMKENPVFRLVLGTCPTLAVTTSAENGIGM
                     GIAATVVLIGSNAVISLLRKVIPDKVRIPAFITIIAGFVTIVQMLLKAYLPAIDKQLG
                     IYIPLIVVNCIILARAEMFASKNGVFMSILDAIGMGIGFTLALLTLGTIREVLGNGTW
                     FGMDISGGVFEPVLIMVLPPGGFLAFGFVLAAINKFSALKVEHKGCASCPMPCGASGK
                     DGEVQM"
     gene            5862..6449
                     /gene="rsxA"
                     /locus_tag="PLA01_02825"
     CDS             5862..6449
                     /gene="rsxA"
                     /locus_tag="PLA01_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HPD00266.1"
                     /translation="MDGYKLMVIVIGALLVKNFVLSQFLGICPFLGVSKKLSTALGMG
                     GAVIFVMTIASGVTRLVYDYLLVPFGLGYLDTIAFILVIAALVQLVEVILKRFVPPLY
                     KALGIYLPLITTNCAVLGVALLNIKEGYNLLESMVYGFSGGLGFTLGLVLFAGVRERM
                     EYCDIPKAFEGLPIALIAAALVSISFFGFQGLMGI"
     gene            6583..7395
                     /locus_tag="PLA01_02830"
     CDS             6583..7395
                     /locus_tag="PLA01_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HPD00267.1"
                     /translation="MLNDLIVPTSIVGGMGLLLGLGLSIASKMFEVKVDERIVRVREV
                     LPGANCGACGQSGCDAFAKSVVSKQSKINGCPVGGQEVADKIGEILGIKAEAVVEKTA
                     RVMCAGTYDTCKVKYDYSGIEDCASAAGLFAGPSACSYGCVGLGNCVKACAFDAIIVE
                     NGLARVIEAKCRACGKCIEACPKKIIEMVSKKGEYAVTCSSMDKGAVVRKNCTVGCIG
                     CGKCSKVCPVGAVTVNGTLAKINSDVCTNCGECAAVCPTGAIKKIICCIGSK"
BASE COUNT         2121 a         1252 c         1966 g         2182 t
ORIGIN      
        1 gacagggtat tcggcgataa gcttcagacg gtgctgattt atatttctgt agtgatttct
       61 atcctattca gtgtcggact ttttatggta ctccccaatg caattgcctg gcttttgccc
      121 attgacgaga ctacaaccat aggatcaatt ctatataata tagtggaagg gataatccgg
      181 gttgcattgt ttatactgta cctttccctt acttcgcagt tgaaggatat taagagggtt
      241 tggcagtacc atggctcgga gcataagaca atacactgct atgaaaatga ggaagagcta
      301 accgtggaaa atgtaaaaaa atattccaca aggcatccgc gttgcgggac atcgttctta
      361 tttacgataa tgatagtaag tataatggtg ttctcattgg tggggcagca tggtttgtgg
      421 ataaacatac tgttgagaat agtgctgatt ccccttgtgg caggggtgtc ttatgagatt
      481 ataaaaattg ccggaaaaag cccgtctaaa attgcccgga ttgtaaacgc accgggattg
      541 atgttccagg gattcacgac ccgggagcct gatgacagcc agattgaagt ggctattgag
      601 gctatgaaga atgtattggt ggagaataaa gaagaagaca agtggtaaca aggtggtagc
      661 atgatattaa aggatgccct tttaaaggga acacaacagc ttaagatggc agatattgat
      721 gccccggcat ttgaggccgg ggcaatatta tgttatatat taaaagtgga ccgtagtttt
      781 ttgtattctc atgatgacta caaaatgacg gatgaggagt acaggtgctt tgatgctttt
      841 ataggcgaga gggccaaggg aaagcccctt cagtatataa ctgggcatca ggagtttatg
      901 ggctttgact ttgtcgtttc tccggatgtg ctcataccga ggcaggacac ggagaccctt
      961 gttgagacgg tgttgaatca tgtgaaggat tcgggaaata aaaaagcaat gattctggat
     1021 ataggcacag gctccgggtg tatagcgata agccttgccg cttatattga aaacagcagg
     1081 gttgttgctt gcgatgtttc agaaaaagct cttgatgtag caaggacaaa tgcccaaaga
     1141 tgcggtgtag agaatcgtgt ggattttata tgcggtgaca ttcttcaagg cctggataag
     1201 gctttgtgtc aaagtacctt tataaaggat aaaaaggaac ataactttga ggcttttgac
     1261 gttattgttt ccaatccgcc gtacataccc gcatgggaaa tacaaaccct tcacaagcag
     1321 gtcaaggacc atgagccggg tcttgcgttg aatgggggag aggacggtct tgatttttat
     1381 agaaaaataa ccgcagaagc ggtacggttt ataaagcccc attccctttt ggcgtttgaa
     1441 gtaggctaca atcaggcaaa ggatgtttcg aagcttatgg aggatggcta tagagacata
     1501 aggactgtaa aagatttgtc cggcatagat cgggtcgtaa ccggtttaag aaagtaggaa
     1561 gatatctctt aaattgttat tgtacgaaaa caaaacctct tattgctttt ctgtcactgc
     1621 ttataatata ggaagataaa agagtaactg cggcaaatat tgcgtgtttt gtatggaagt
     1681 tattccggct attatggatg aagcatcctt gccatgtatg gattgtattg ggaattccaa
     1741 gctctattag tatgtatatg ataaagcccg cacaaaaatc cttgaagaag aaaatataaa
     1801 attgtataca atagacatat tcgatttgtg gaatgctgta atggcaaggg tttgacaatt
     1861 atttaacaat aaaaaatttt cataacagat attgatatca ttttctaaaa aagactagaa
     1921 taaataagca atagagacat atttaataaa aatggctgtt tgacttacag caaataagta
     1981 aatataatta ttatggctta agaagattct aggcataacc actagttatt caaacgactg
     2041 atatttatag tcgaagatag agtaatagaa tgtgcattcc acagcacgca agtacttaca
     2101 taaaatggtg aaagatacag tctttttaac atgtaattaa aatattcttt gtggtattat
     2161 tgtaatatct cttaaaagaa ttgatttctg atagatttag gggatttatc atttaacatt
     2221 cgattgtgct gaagaaaact tacataataa agtggttatt ttataaaata ttacttaaat
     2281 ggggtcgtat aaatgtttgt taagagattc tttaaaggtg gcacaaatgt acctcactac
     2341 aaaaacactg ccaactgtga aactgttaaa atgggagttc cggaaaaggt aataattcca
     2401 atgattcaac acatcggggc accgtgcgag ccttgtgtac aaaagggaga caccgtaaag
     2461 gtggggcaga ttataggaga ttccgggaag tttatatcag cacccgttca ttcaagtgtt
     2521 tccggtacag tggttggagt ggcaccgatg cttcaatccg gaggttttta tgttccggcg
     2581 gtggaaattg aaaccgatgg aaaacaggag gtatatgagg ggatcaagcc gccgaagtat
     2641 tcaaacatta aggagtttat tgaagcaata aggcaatcgg gccttgtagg tctgggaggt
     2701 gcggggtttc cggcacatgt caagctttct ccgcccccgg ataagaaaat tgacactctt
     2761 gtcataaacg gtgccgaatg cgagccgttt atcacgtctg attacagaga gatcatcgag
     2821 aactcatgga atgtcatcag cggtatcaat atagtcatgg agattctaaa cataaataac
     2881 gtcataatag gtatcgaaga caacaaaccc gaggcaatcg aggttatgga acgaattgac
     2941 aagtccagtg acaaaataag tgtggttaag ctcaagtcgc ggtaccccca gggtgcggaa
     3001 aagatgctta tatacgccac aacaaggagg aaggttcctg ccgggaagct tcctgccgat
     3061 gtgggtgtta ttgtcatgaa tgtgaacagt gtttccttca tttccgagta tgtaaaaacc
     3121 gggatgccct tgataaagaa aagggttacg gtggatggcc cgtcggttaa aaaccccggc
     3181 aatgtggagg ttttgatagg tactcctatt cataaggttt tcgatttctg cggcgggttt
     3241 actgtaccac cgaagaaggt gttgatggga ggacccatga tgggactggc ccagtattcg
     3301 ttggatttat cggtagttaa gcatacaaat gccttgctgg cttttgatga taaaagttca
     3361 aagatacctg aatccggagc atgtatacgg tgtggaagat gtgtttgggc ctgtcccatg
     3421 aacctaatgc ctttatgtct ttataataat gccataaagg aaaacataga tgaactcaac
     3481 aagtaccatg ttaatgattg tattgaatgc ggatgctgct cttatgtatg tcctgcaaag
     3541 attaaccttg tgcagtcttt aaggcttggc aaggcaaagg taaaacaagc agcagcggcg
     3601 aaagggggga aataatttgg aagacagatt gacggtatca tcctcacccc atataaggga
     3661 taaggcaaac acccaaagga taatgataga tgtggttata gcccttcttc cggcgacatt
     3721 tgcaggggta tacttttttg gaacaagggt tttacttgtg gttatggtca gcattctatc
     3781 ctgcgttgta tcggagtatt tatcccgccg gataatgaaa aggagtaata caatttcgga
     3841 ttttagtgct gtggtgacgg ggttgcttct ggcattgaat cttcccccga ctgttcccct
     3901 ctgggttgca gcagtgggca gtgctatagc gatagtggta ataaagcaat tgtttggcgg
     3961 gttgggacag aattttgtca atcctgcatt gggggccaga attattttga tgatttcgta
     4021 tccgggattt atgaccaact gggtaaagcc cggtgtggat gcggtttccg cggcagcttc
     4081 cggtgtggat gcgatttctt cggcaacacc acttcaaata atgaagtatg gagcgtcgat
     4141 agcggatttg ccgtcctatg gggagctttt ctggggcaat ataatgggat gtctagggga
     4201 aacctctgcg gcagcccttt taataggtgc ggtgtacctt tttgcaagaa agattattag
     4261 tccggaaatt ccctttgcgt tcatcggaac tacagctttg tttacatgga tctttggagg
     4321 tactacgctt tttaccggcg acttcttgtt ccatgtattg gccggaggat tgttgctggg
     4381 ggctatttat atggctacag actatgcgac atctccaatg accgtaaaag ggcggattat
     4441 tatggggatc ggctgcggtc ttcttacggg aattataaga ttgtttacca actacaccga
     4501 aggggtatcc tttgcaatag ttcttatgaa catcatggtg cctctcattg acagatatac
     4561 cataccaaag agctttggag gtgaaaaagc cgttgcgtga tatagctaaa cctacagtgg
     4621 tgttattttt gatatgtacg attgttactt ttgcccttgc ttataccaat gcaattactg
     4681 ccgacaaaat agatgagatg ataagtcttg agctggacaa tgccagaagg aaagtgttga
     4741 gcttggccga cagctttgaa aaaatagaag ggcttgagga gatagtaagc gaaaatgctg
     4801 atcttggtat tgtaaaggaa gcctataaag gtgtaaaggg tcaggagacc gtcgggaacg
     4861 tatttttggt ggaaagtaag ggatatggcg gtgccattac aataacggtg ggtatagaca
     4921 ataagggtaa aataaccggg gtcgagatag gagacaataa tgaaactccc gggcttgggg
     4981 ctaaggctaa ggatgagccc tttatatccc agtttgtggg cataataccc gaagaaccgt
     5041 taacggttgt aaaaagcaat aagtccaaaa gcgaggagat agatgcgata agcagtgcaa
     5101 ccataacttc aagggctgtg acaaaagcgg ttcaggcagc tgtggatatt aatgctgagc
     5161 ttatgaaacg aggggggaat tcctgaagtg agtgcggtaa aagagggttt taagaatctg
     5221 acgaggggaa taatgaagga aaatccggtg tttaggcttg tacttgggac ttgtcctaca
     5281 ctggcggtta ctacatctgc agaaaacgga atcggaatgg gaatagctgc tacagttgtg
     5341 cttataggct caaatgcggt gatttcactt ctcagaaaag taattccgga taaagtaagg
     5401 attcctgcct ttatcacaat tattgccggt tttgtgacca tagtgcagat gcttttgaaa
     5461 gcgtacttgc cagcaatcga taaacagctc ggcatatata ttccccttat agttgtaaac
     5521 tgcataatac ttgcccgggc agagatgttc gccagcaaga acggtgtgtt tatgtctata
     5581 ctggatgcta ttggcatggg aatcggtttt actttggcat tgcttacttt gggcaccata
     5641 agggaagtat tgggtaatgg aacctggttc ggaatggata tttcaggagg ggtttttgaa
     5701 cctgtattga taatggtact gcctcccgga gggtttcttg cctttggatt tgtattagca
     5761 gcgataaaca agttttcggc gttaaaggtt gagcataagg gttgtgcatc atgcccaatg
     5821 ccttgcgggg cttcgggcaa agacggggag gttcaaatgt aatggatggt tataaattga
     5881 tggttatagt tattggtgca cttttagtta agaattttgt gctgtcgcag tttttaggta
     5941 tatgtccctt cctcggcgtg tccaaaaagc tttcaacagc tcttgggatg ggtggtgcgg
     6001 ttatatttgt aatgaccata gcctccggtg tgacacgtct ggtgtatgat tatttgcttg
     6061 ttccgtttgg gttgggatat cttgatacta tagcatttat ccttgttata gctgctcttg
     6121 tgcaattggt tgaagtgatt ctaaaaaggt ttgtacctcc tctttacaag gctttgggta
     6181 tttatcttcc ccttatcacg acaaactgtg ccgtattggg ggttgctctt ttgaatatta
     6241 aagagggata taatttactg gaatcgatgg tttacgggtt tagcggaggc cttggattta
     6301 ccttgggact ggttttgttt gcaggggtca gggaaagaat ggagtattgt gatataccaa
     6361 aggcgtttga aggacttccc attgcattga ttgctgcagc cttggtttcg atatcctttt
     6421 tcggtttcca gggactaatg ggaatataat agggtaatgg atgtaagaag ttttttatac
     6481 aaacgaaaat cttctttgaa ctttgatgta taaggctgat tttgggctat ggagacgagg
     6541 tttcttggct tttgttcaat ctttaagagg ggttgttgag atatgcttaa tgatttgata
     6601 gtaccgacat caattgttgg aggaatgggt ttactgttgg gattgggtct ttccattgct
     6661 tcaaagatgt ttgaggttaa ggtggatgag cggattgtaa gggtaagaga ggttttgccg
     6721 ggggcaaatt gcggtgcctg cggtcaaagc ggatgcgatg cgtttgcaaa gagtgttgtt
     6781 agcaagcaga gcaaaataaa cggttgtcct gttggcggac aggaggttgc ggataagatt
     6841 ggagaaatcc tgggtattaa agccgaagct gtagtggaaa aaaccgcaag ggttatgtgt
     6901 gccggtactt atgatacgtg taaggtaaaa tatgattatt ccggaattga agattgtgct
     6961 tcggcagccg gcctttttgc aggtccttcg gcatgttctt atggctgcgt tggtcttggc
     7021 aattgtgtaa aggcctgtgc ttttgacgca ataattgtgg aaaacgggct tgcccgggtt
     7081 atagaggcaa agtgcagggc atgtgggaag tgtattgagg catgtccaaa gaagataatt
     7141 gaaatggttt ccaaaaaagg tgagtatgct gttacatgct caagcatgga taaaggtgcc
     7201 gtggtcagga aaaattgtac tgtcggctgt ataggctgcg gaaaatgttc aaaggtatgc
     7261 ccggtgggtg ctgtaacggt taacggtaca ttggcaaaga ttaattccga tgtatgtacc
     7321 aactgtggtg agtgtgcggc cgtctgtcca accggggcta ttaaaaagat tatatgctgc
     7381 atcggttcta aatgacatag gaggaatgac acgaggggac ggttcttgcg tgacacgagc
     7441 acagaagagc cgctcccttc tgtatattta gcggattttc tgcatccatg acacggaaga
     7501 accgtccccc tgtgctatta t
//
LOCUS       DAOOJC010000061         7480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_8319_length_7480_cov_3.487003, whole genome shotgun sequence.
ACCESSION   DAOOJC010000061 DAOOJC010000000
VERSION     DAOOJC010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8319_length_7480_cov_3.487003"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1842
                     /gene="fusA"
                     /locus_tag="PLA01_02835"
     CDS             <1..1842
                     /gene="fusA"
                     /locus_tag="PLA01_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPD00268.1"
                     /translation="NIIDTPGHVDFTVEVERSLRVLDGSVTVFCAKGGVEPQSETVWR
                     QADRYNVPRIAYINKMDIMGADFFNCIKMMKDRLQANAVPIQLPIGKEDNFQGIIDLV
                     KMTAYFYMDDLGKDIQEREIPDDMKELAEEYRTNLVEAVSDQDETIMMKYLEGEEITE
                     EEIKTAIRKATLNVKIVAVTCGSSYKNKGVQRLLDAVVDYLPSPLDIDAIKGATMDGD
                     EVERPADDNAPFSALAFKVMADPYVGKLCFFRVYSGQLSSGSYVLNATKGKKERIGRI
                     LQMHANHREEIDRVYSGDIAAAVGLKDTTTGDTLCDDANPVILESMEFPEPVISVAIE
                     PKTKAGQEKMSIALQKLSEEDPTFRTYTAPETGQTIISGMGELHLDIIVDRLLREFKV
                     EANVGNPQVAYKETIRKVIKAEGKYVRQSGGKGQYGHCWIELEPREPGAGYEFVNKIV
                     GGVIPKEYIPAIDAGIQSAMNTGILAGYPVVDVKVTLYDGSYHEVDSSEMAFKVAGSM
                     AFKEGMRKADPVLLEPIMKVMVTVPEEYMGDVMGDLNSRRGRIEGMEARSGAQVITVN
                     VPLSEMFGYATTLRSRSQGRGVFSMEISHFEEVPKNIQEQIIDGKKG"
     gene            1979..3181
                     /gene="tuf"
                     /locus_tag="PLA01_02840"
     CDS             1979..3181
                     /gene="tuf"
                     /locus_tag="PLA01_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPD00269.1"
                     /translation="MAKAKFERSKPHVNIGTIGHVDHGKTSLTAAITKVLGFQGKADF
                     TSYENIDKAPEERERGITISTSHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKCDMVDDEELIELVEMEVRELLSS
                     YEFPGDDIPIVRGSALVALESSSTDINSPEYQPIVKLMEEVDKYIPTPERATDKPFIM
                     PVEDVFTITGRGTVATGRVERGTLKMGEEVEIVGLMDEPRKTVITGIEMFRKLLDQAV
                     AGDNIGALLRGIQRTDVERGQVLAKPGSIKPHTHFEGQVYVLTKEEGGRHTPFFNNYR
                     PQFYFRTTDVTGVIELPEGTEMVMPGDHITMKIKLITPIAMEEGLKFAIREGGRTVGA
                     GNVSKIIE"
     gene            complement(3281..3652)
                     /locus_tag="PLA01_02845"
     CDS             complement(3281..3652)
                     /locus_tag="PLA01_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPD00270.1"
                     /translation="MIVENGTKGSVLSMNTIIADTFAKRFTGLMFKDSLPDNSGLLIE
                     PCNSIHMFFMKFSLDIVFIDREDRVIHLIESIPPWRCSRIVFGSRRVLELPVGTIKKS
                     DTCIGDKLLFVKTGEGKYFET"
     gene            3934..4488
                     /locus_tag="PLA01_02850"
     CDS             3934..4488
                     /locus_tag="PLA01_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPD00271.1"
                     /translation="METPDVDLISKCLNGDNDAFSGLVTRYKKLIYSVVYNMINNKQD
                     VNDIVQEVFIKIYSALDKYNPEYKFSTWAVRIATNCCLDILKKKRIDAVSIEEKIGVS
                     DSVDTPEDEYIKKEKRKRINDELNRLPEKYRVPLILFHKNGLSYDEISQVLNEPMSIV
                     KNRLYRARLMLRDSLSSEREEGVL"
     gene            4485..5039
                     /locus_tag="PLA01_02855"
     CDS             4485..5039
                     /locus_tag="PLA01_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPD00272.1"
                     /translation="MSCEQFEDSIMKHIDGEINDIENNRLMKHLRECAECSRKLEELK
                     SIMGTLEQTDVIEPPFDLEQTVMEKVKKLDVYNKKLKEKKLMMSYFAALMIFTVILLL
                     SAILFRENLSEFMLFIGIPKPVTYMVYGYLTGLGSFIVMVASCIKELKDEIADFNYLL
                     AGLFAIVLITKIYEINSAQRKNEG"
     gene            5418..5780
                     /locus_tag="PLA01_02860"
     CDS             5418..5780
                     /locus_tag="PLA01_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00273.1"
                     /translation="MDKDFMKNDEIIEESRNEHNDEAQQRRLFDNNEQVNSGDKGALA
                     LERIDRLYVVLGWISAAVTIFVSPLFAIAGVTFGVLLNRQHKGSGNVIIISNIALALI
                     NIALSQVILMLINNMMYR"
     gene            5895..6542
                     /locus_tag="PLA01_02865"
     CDS             5895..6542
                     /locus_tag="PLA01_02865"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HPD00274.1"
                     /translation="MQTRYRKIVIVFVTVYLFAFVMGCNEKEEERVKDDVQFQNINPQ
                     ITGGDIKNPIAEDSNNGLIEIGSFSTSLLDDQKNRVENLHIAADCLNNERVSPGEEFS
                     FNVTLGRRTEEKGYKKAPIIKRTKDGPKKGYGVGGGICQLSSTLYNAAQKAGLKITEI
                     HHHSKDVGYVPEGKDATVVYGSKDFKFINNRSNPIVIKASVSEEKVAVSIFEMLK"
     gene            6757..7026
                     /locus_tag="PLA01_02870"
     CDS             6757..7026
                     /locus_tag="PLA01_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPD00275.1"
                     /translation="MVKKEITITSKTGLVAKPAALFIHKASNYKSSIWIEKGERKANA
                     KSLLGLLALGVDYGSKIVLIAEGDDEKEAVDELCEHLSTKLEESV"
     gene            7029..>7480
                     /locus_tag="PLA01_02875"
     CDS             7029..>7480
                     /locus_tag="PLA01_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HPD00276.1"
                     /translation="MGRKTFKAEAVSFGIRIGRIFIIKEADVKTDENLIDESQIENEI
                     TNLEVAICKTFIEIHDLNDGFKGILSEEENRIFDFYTQILDDKRFFEEIKDTIKHKKY
                     SAQKAIHTCIQKYIDEILKSNSDYLRQRAYDLNDVRKRLIKNISGSSG"
BASE COUNT         2556 a         1157 c         1682 g         2085 t
ORIGIN      
        1 aatattattg atacacccgg tcacgtagat tttacagttg aagttgagag atcacttcgt
       61 gtgcttgatg gttcagtgac tgttttctgt gcaaaaggag gagttgagcc tcaatctgag
      121 actgtatgga gacaggccga tagatataat gtacctcgta ttgcatatat taataagatg
      181 gatataatgg gagcagattt ctttaactgc ataaagatga tgaaggacag actgcaggcc
      241 aatgcagttc ccattcagct ccccattgga aaagaagata acttccaagg tatcatagac
      301 ctagttaaaa tgacagccta tttttatatg gatgaccttg gaaaggatat tcaggagaga
      361 gaaattcccg acgacatgaa ggagttggct gaggaatacc gtactaattt ggtagaggct
      421 gtttcagacc aggatgaaac aataatgatg aagtatcttg agggtgaaga gataacagaa
      481 gaagaaataa agacagctat aaggaaggcc actttaaatg taaaaatcgt agctgtaacc
      541 tgtggttctt cttacaaaaa taaaggtgtt caacgcctac ttgatgcagt tgttgattat
      601 ttaccttcac cgttggatat agatgccata aaaggtgcga ctatggacgg agacgaagtt
      661 gaaagaccgg cagatgacaa tgcaccgttt tctgcattgg catttaaggt tatggcggat
      721 ccatatgtag gaaagctttg tttcttcagg gtttattcag ggcaattgag ttccggatcc
      781 tatgttctta atgcaacaaa aggaaaaaaa gagaggatag gaagaatatt gcagatgcat
      841 gccaaccaca gggaggaaat tgacagggtt tattccgggg atatagcagc tgcagtcggt
      901 ttaaaggata ctactaccgg agacactctt tgtgatgatg ctaatcctgt aattctcgag
      961 tccatggagt ttcccgaacc tgttatttca gttgcgattg agccaaagac aaaggcagga
     1021 caggagaaga tgtcaatagc acttcagaag ctttctgagg aggatcctac ttttaggacc
     1081 tatacagctc ctgaaaccgg acagactatt atatccggta tgggagaatt acatctggat
     1141 attatagtag accgtttgct cagggagttt aaggtggaag cgaatgtcgg taatccacag
     1201 gttgcttaca aagaaaccat cagaaaggtt attaaagctg aaggaaaata tgtaaggcag
     1261 tccggtggta aaggtcaata cggacactgt tggattgaat tggaaccgag agaacccgga
     1321 gctggatatg agtttgttaa caaaatagta ggtggcgtta ttccgaagga atatattcca
     1381 gctattgatg caggaattca aagtgctatg aacaccggta ttcttgcagg gtatccagtt
     1441 gtggatgtaa aagttacgtt gtatgatggt tcataccatg aggttgactc ctcggaaatg
     1501 gcatttaagg ttgccggctc catggcattc aaggaaggta tgagaaaagc cgatcctgta
     1561 ctattagagc cgattatgaa agtaatggtt actgttcctg aagagtatat gggtgacgtt
     1621 atgggagatc tgaactcaag aaggggaaga attgagggca tggaagcaag gtcaggtgca
     1681 caggtaatta ctgttaatgt gcctttgtcc gaaatgtttg gatatgctac cacattgcgt
     1741 tcaagatctc agggaagagg cgtgttctca atggagataa gccacttcga agaagtacct
     1801 aaaaatattc aagagcagat tattgatggt aaaaaaggct aatgcagaat gaaatattct
     1861 gaagaaaaac aggaagcata caaatatatt tgcatgtttc ctgttgaatg atatttactt
     1921 aaataaatta aattaataat tatcagttgg ttaactgaaa aaaggaggag aatttataat
     1981 ggcaaaagct aaatttgaaa gaagtaaacc ccacgttaat atcggaacaa taggtcacgt
     2041 tgaccatggt aagacctcgt tgactgcagc aattacaaag gttttgggct tccagggtaa
     2101 agcagatttt acttcatatg aaaatattga taaagctccg gaagaaagag aaagaggaat
     2161 taccatttcg acatctcatg ttgagtatga aacagataca aggcactatg cacacgttga
     2221 ctgccccggc catgctgact atgttaagaa catgataacc ggagctgctc agatggatgg
     2281 tgctattctg gttgtatcgg ctgcagacgg tccgatgcct cagacaagag aacacattct
     2341 tttggctcgt caggttggtg tgccatacat agttgtgttc ttgaacaagt gtgatatggt
     2401 agatgacgaa gaacttatcg aattggttga aatggaagtt agagaactgt tgagctctta
     2461 cgaattccct ggagatgata ttccgatagt aagaggttcc gcacttgttg ctcttgaaag
     2521 ttcatcaacc gatattaatt ctcctgaata tcagccgatt gttaagctga tggaggaagt
     2581 tgataaatac attccgacac cggaaagagc tacagacaag ccgttcatta tgcctgttga
     2641 ggacgtattc acaatcacag gccgtggaac tgttgctaca ggtagggttg aaagaggaac
     2701 tcttaagatg ggtgaagaag ttgaaatcgt tggtttaatg gacgaaccga gaaagacagt
     2761 tattaccggt atagaaatgt tcagaaagct tcttgaccag gctgttgccg gtgacaacat
     2821 cggtgctctt ttaagaggta ttcagagaac tgatgttgaa agaggacagg ttttggctaa
     2881 accgggatca ataaagccac atactcattt tgaaggacag gtttacgttc ttactaaaga
     2941 agaaggtgga agacatactc ctttcttcaa caattacaga cctcagttct atttcagaac
     3001 aactgacgtt actggtgtaa tcgagcttcc ggaaggaaca gaaatggtta tgcctggtga
     3061 tcacattaca atgaagatta agttgataac tccaatagct atggaagaag gcttgaagtt
     3121 cgctatccgt gaaggtggaa gaactgttgg agcaggtaac gtttcaaaaa taattgagta
     3181 ataaaataag aaaatccgga gggcattgcc ccccggattt tttagtacat tgatttaaat
     3241 gtactgtaat tactgaggtg aaggaaaata tttcgcaaat ttatgtttcg aagtattttc
     3301 cttcacctgt tttaacaaac aaaagtttgt cccctataca tgtgtcggat ttctttattg
     3361 taccgacagg caattccagc acacgccggg aaccgaatac aatcctggag catctccacg
     3421 gcggaatgct ttcaatcaaa tgtatgaccc ggtcttcacg gtctataaac actatgtcca
     3481 gcgaaaattt cataaagaac atgtgtattg agttgcaggg ttcgatgaga agacctgaat
     3541 tgtcaggcag agagtcttta aacatcagtc ctgtaaaccg ctttgcaaag gtgtctgcaa
     3601 taattgtgtt catggaaaga acagaacctt tggttccatt ttcaacaatc ataagatacc
     3661 cctccagaaa tatccggata tataccgggt ttattgtatt taaaacggta atgataaatc
     3721 agacaaattc gataaattaa cgtataattt attaatgggc aattaagact gaagatgata
     3781 tgttatagat atattttatc ataaataaaa caaatttcaa taaaataccc tgaaaattat
     3841 aactgcttct aaaatattca agtttattct gtcgtgctgt gataaaatgg aagggggaat
     3901 tggttttcag tgcggtaagg aggtgacaca cagttggaaa cacctgatgt tgacctgata
     3961 agtaaatgtc ttaacggtga caatgatgca ttttcaggat tggtgacgag atataagaag
     4021 ttgatttata gtgtagtgta taatatgata aataataagc aagatgttaa cgatattgtt
     4081 caggaggttt ttattaaaat atacagtgct ctcgataaat ataacccgga atataaattt
     4141 tcaacctggg ctgtcagaat tgcaacaaat tgctgtttgg atattcttaa aaagaaaaga
     4201 attgatgcgg tatccatcga ggagaaaatt ggtgtttccg acagtgttga tactccggaa
     4261 gacgaatata taaaaaagga gaagcggaag agaataaatg atgaattaaa ccggcttccc
     4321 gaaaagtatc gtgtaccttt gatattgttc cacaaaaacg gactgtccta tgacgaaatt
     4381 tcacaggttc tcaatgaacc gatgtctatt gtaaaaaaca ggttataccg ggcgagactt
     4441 atgttaagag atagcttatc ttccgaaaga gaggaagggg ttttatgagt tgtgaacaat
     4501 ttgaagattc cattatgaag catatcgatg gagaaattaa tgatattgag aataaccggc
     4561 ttatgaagca tttaagagag tgtgccgaat gcagcagaaa gcttgaagaa ttaaagagca
     4621 ttatgggcac tttggaacag acggatgtca ttgagcctcc tttcgatttg gaacaaactg
     4681 ttatggaaaa ggtcaaaaag cttgacgtgt acaataaaaa gctcaaagaa aagaagctga
     4741 tgatgtcata ctttgcagca cttatgattt ttacggttat acttttgctg tctgcaatcc
     4801 tgtttaggga aaacctgtca gagtttatgc tgtttatagg aattccaaag cctgtaacct
     4861 atatggtgta tggctatctt accggccttg gttcctttat tgtaatggtt gcatcctgta
     4921 taaaagaact taaggatgaa attgcagatt ttaattatct tttggctgga ttgtttgcaa
     4981 tcgtcctaat tacgaagatt tatgaaataa attcggcaca gagaaagaat gaagggtgaa
     5041 taaaaatgta atgaaaaatc atgtaaagaa aaatattcca gaaacttaag ttacgctgtc
     5101 tagagtctgt aaatactccg tggggtcgag caaaactcgc ttcgctcgga caatgctctc
     5161 cctttccacg tcgtattaac agactctatg acaggcttca taatgtttct tccacatttt
     5221 tcattacatt gacagtatgc acttaattaa cctgggttgt tgtcaaattc aaaaaagcaa
     5281 aaattaataa ctgcttaaat attaacaata aaacgaagga tttctattaa gacttcgttt
     5341 tttttgttta agaaaattat acttgaatta tatattcaca taactctaat aataaataat
     5401 cggacggagg tgattcgatg gacaaagact tcatgaaaaa tgatgaaatt attgaagaat
     5461 caagaaatga gcacaatgat gaagcacagc aacgaagatt atttgataat aatgagcaag
     5521 taaacagtgg tgataaaggt gcattagccc ttgaaagaat cgacaggctt tatgtcgttc
     5581 taggatggat aagtgcggct gtaaccatat ttgtatcacc gctgtttgca attgcaggag
     5641 taacctttgg agtattgtta aacaggcagc acaagggaag cggaaatgta atcattataa
     5701 gcaatattgc actggcattg attaatatag cactcagtca ggtaattctg atgctgataa
     5761 ataacatgat gtaccgttaa ctaagggttc attaaataaa tgtgaagaaa aatattcgag
     5821 aaacttaagt ttcttccata tttttcttca ctgtttctaa agtcctatta ttattgacgc
     5881 ggaggaaaac tagtatgcaa acaagataca gaaagatagt tattgttttt gtgacggttt
     5941 atttgtttgc ttttgttatg ggctgcaatg aaaaagaaga agaaagggta aaggacgatg
     6001 tccaatttca aaacataaat ccacaaatta ctggtggaga tataaaaaat ccgattgcag
     6061 aggattcaaa caatggcttg atagagatag ggagcttctc aacctcgcta ttggacgatc
     6121 agaaaaacag ggttgaaaac cttcatattg cagcagattg tcttaataat gaaagagtat
     6181 cgccgggtga ggagttttcc tttaatgtga ctttaggaag aaggacggaa gagaagggat
     6241 ataaaaaggc acccataata aaaaggacga aggatggtcc caaaaaagga tacggagttg
     6301 gaggaggcat atgccaattg tcgtcaacat tatataatgc agcacagaaa gccggattga
     6361 aaattaccga aatacaccat cactcaaaag atgtcggcta tgttccggaa ggcaaagacg
     6421 ccacagtggt atacggatcc aaggatttta aatttataaa caaccgttcc aacccaattg
     6481 taattaaagc ctcggtatcg gaggagaagg ttgcggtaag tatatttgaa atgttaaaat
     6541 agtattatta accaaacaat aataacagca ggcaattaag cagcaataaa ggtacttaat
     6601 aataatataa accgtggttt tcataaaaaa ctatggaact attcttaaaa taatgatata
     6661 aatataacga ggcgataaat gttcccaact tctataagtg gtgggtacag attttaataa
     6721 atgtaaaact cggttatttg ggaggatata agtcacatgg taaaaaagga aattacaatt
     6781 acgagtaaaa caggattggt agcaaagcct gcagcactgt tcattcataa ggcgtcaaac
     6841 tataaatcga gtatctggat cgaaaaaggt gagagaaagg ccaatgccaa aagtttactg
     6901 ggccttttgg cattaggggt tgattatggc agcaaaattg tacttattgc ggagggtgat
     6961 gacgaaaaag aagcagttga tgaattgtgc gaacatcttt caacaaagct ggaagaaagt
     7021 gtttagttat gggtaggaaa acattcaaag ccgaggcagt ctccttcggc attagaattg
     7081 gaagaatatt tatcattaaa gaggcagatg taaaaaccga tgagaatctg atagatgaat
     7141 cccagatcga aaatgagatt acaaatcttg aggtcgctat atgcaaaaca tttatcgaaa
     7201 ttcatgattt gaatgacggg tttaaaggaa tacttagcga agaggaaaac aggattttcg
     7261 atttttacac gcaaatactt gatgataaaa ggttttttga agagattaaa gacactataa
     7321 agcataagaa gtacagtgcc caaaaggcaa tacatacgtg tattcaaaag tatatagatg
     7381 aaatactcaa aagcaatagt gattatttaa gacagagggc ttatgacctc aatgatgtca
     7441 ggaaaagact tattaagaac atttccggca gcagtggaat
//
LOCUS       DAOOJC010000062         7394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_8432_length_7394_cov_3.741382, whole genome shotgun sequence.
ACCESSION   DAOOJC010000062 DAOOJC010000000
VERSION     DAOOJC010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8432_length_7394_cov_3.741382"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLA01_02880"
     CDS             complement(<1..223)
                     /locus_tag="PLA01_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPD00277.1"
                     /translation="MLSSELLKKIRQIEIKSNKLVDEVFSGEYRSGFRGKGVEFEDIR
                     EYYPGDDVRSIDWNVTARHNKAFVKQFCEE"
     gene            complement(248..1201)
                     /locus_tag="PLA01_02885"
     CDS             complement(248..1201)
                     /locus_tag="PLA01_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPD00278.1"
                     /translation="METTEFLDEIKILTDLKEEIGKIIVGQKYMVDRILIGLLTGGHI
                     LLEGVPGLAKSLTANTIAQVVGIDFKRIQFTPDLLPADILGTEIYNQKTGEFIVKKGP
                     IFSNLILADEINRAPAKVQSALLEAMQEKQVTIGEKTYHLDNPFLVIATQNPLEQQGT
                     YPLPEAQQDRFMMKLKIKYPGRDEESRIIDFYSKEQPADPDVRKVLSREDIFRLRKKV
                     DGIYIDDNIKNYVLDIVLKTREESEYIACGASPRASINLIKAAKGRAFLEGRNFVVPD
                     DIKAIAYDVLRHRILLTYEAEAEDIASEKIIANILEQVNLP"
     gene            complement(1332..2315)
                     /locus_tag="PLA01_02890"
     CDS             complement(1332..2315)
                     /locus_tag="PLA01_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HPD00279.1"
                     /translation="MDEHSVEVQEQYDVEKPRIWQVGLLYSLVVILLVFVSTKLQEAL
                     QFNIGGLISEVFLIMLPPLVFLFIFRFDTKKVLRWNRTSFVNFVLAFGIVIFGIPIVG
                     VFNLLNILIVKLLSGTVETTQIPIGSGVGDLFIGVLVIAISAGICEEVLFRGVIQRSF
                     ERFGSVKSILITAFLFGLTHFSFQSLFGTFLLGALIGFLVYKSDSLMIGMFAHFTNNA
                     VAVVLNWVSIKMLEFLEKMGVENVNNPAGQDVFSQFQGMSIPEIVFMAAVALAVVSFS
                     AAVFGVLVYWFIKLNSKNVEKPRENQTRVSIIEFIPLIPGLLLVAYICINK"
     gene            complement(2682..4076)
                     /gene="ypeB"
                     /locus_tag="PLA01_02895"
     CDS             complement(2682..4076)
                     /gene="ypeB"
                     /locus_tag="PLA01_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="germination protein YpeB"
                     /protein_id="HPD00280.1"
                     /translation="MSIREKLLDLKRRLSDRKMYSIVIVAIAAVAMWGFYQYKHAADL
                     RQELDNQYNRAFYDMVGYVNNVEVLLIKSLIANTPEKTAATMQEAWRQANLAQTNLGQ
                     LPVSPNVLANTSKFLTQVGDLAYSLNNQNMGGKGLNEEQYKLIEQLHGFSVSLEQSLI
                     DLQNQITTGRIKWGDLANKGTRLFQKTSENMPNAQFENIEKTFQEYPTLIYDGPFSDH
                     MTQTEPKGLAGEDMDVEQAKQRVMEFFGPDKVKAVEEIGRVDEGNIKTYSYSVKFNNV
                     PEDQTATVDVTQKGGNVLWMLYNRPVGAQEQINVDQAKALGKKFLEEHGYKDMVDTYY
                     LKEDNTAIINYAYKQGDVTVYPDLIKVKIALDNGEIVGFESKGYITNHTQRNIPQPKI
                     TMEQARALITPRMNVLSSGLAIIPTDYKTEIFTYEFKGKLNDRDFLVYINAENGKEEN
                     ILLIMDTPNGILTM"
     gene            complement(4095..4790)
                     /gene="sleB"
                     /locus_tag="PLA01_02900"
     CDS             complement(4095..4790)
                     /gene="sleB"
                     /locus_tag="PLA01_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic enzyme"
                     /protein_id="HPD00281.1"
                     /translation="MKTVKRIIILVVIFSVSIPLFLKGSVFIAPITGKLAPDMEGDDV
                     RKMQQRLKNWNYYDGEISGKYDLKTYFAVRDFQKTYNLPVSGIANEDTLTAMGLTTLA
                     KWGEVYAQSNQTSNEQLLARAIYGEARGEPYEGQVAVGAVILNRTRDPKFPKTVAGVI
                     YQPGAFTAVSDGQINVPIDPNSTVVKAARDALNGWDPTNGCLYYWNPATATSKWIWSR
                     QIVKKIGKHYFGK"
     gene            5243..6385
                     /locus_tag="PLA01_02905"
     CDS             5243..6385
                     /locus_tag="PLA01_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD00282.1"
                     /translation="MRILFLSISMGAGHIKAAEALKEYIVLRYPGSENLIVDTLKYIN
                     PVVHKVVVDGYLNVIKKKPEIYGCLYRMSEYRENITRLSQRVSSLLASKIHKLVLDFK
                     PAIIVCTHPFPLQMVAHLKKHKNMKVPAVAILTDFVNHPLWLHDNIEAYIVGHDYIKH
                     DMEECGIDKNCIFSYGIPVSRKFLKPTSKATARNRLSLENKLTVLLMGGSLGIGEIES
                     SFRAFAGCKRDLQIITVAGKNAVLEEKIKALSKSYNKPIRTLGYTQNIPELMDCSDFI
                     ATKPGGMTISEALVKKLPILIISPIPGQEERNARFLINSGAAVAINKKDSIDGTLCQV
                     FDNPLRYRHMSQMAENLSTPEAGHNIVNLMEKLIHDNLFKYSSNTL"
     gene            complement(6356..7381)
                     /locus_tag="PLA01_02910"
     CDS             complement(6356..7381)
                     /locus_tag="PLA01_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPD00283.1"
                     /translation="MKRTLILLLNITMLYMVLCSCDQPVDNTIEIPSPEVTAGGEAIS
                     THGKGEDTTIPSDEGDKIKGGSEGVSYDNVAVVLDTVVDVFREPDITSERVTQAIFNQ
                     PVTILEEKDSWVKVKVVDGYTGWLRSKYIDRDCSSIMKENFTDKAVVTEKTQKVYSGA
                     GGGVTLKDVVMGTELYIKNKKDRYFEVVLPGNITGWIETKGTIKVPVESPIPKTSAQD
                     FLATVKKFMETQYLWGGVSAWQGLDCSGLVYICSRINGVNLPRDADMQYDFIKTGPQN
                     TGGLKPGDLLFFSSNEDLANVSHVGIYLGEDQFIHASQSAGKVTYGSINSEYYKKRLK
                     GIRRIFE"
BASE COUNT         2138 a         1742 c         1305 g         2209 t
ORIGIN      
        1 tttcctcaca gaattgtttt acaaaggctt tattatgccg ggctgttaca ttccagtcaa
       61 tacttcggac atcatccccg ggataatact cacgaatatc ctcaaattcc actcctttgc
      121 ccctgaaccc agaacggtac tctccggaaa aaacctcatc caccagctta ttcgatttta
      181 tctcaatttg tcttatcttt tttaacagtt cactcgaaag catatttgcc tccaataatc
      241 ctataaatca aggtaaatta acctgctcga gaatattggc aattatcttt tccgaagcta
      301 tatcctcagc ctctgcttcg tatgtaagca ggattctatg gcgaagaaca tcgtaggcaa
      361 tggctttgat atcatcgggc acaacgaaat tcctgccttc taaaaatgcc ctgcctttag
      421 ctgccttgat cagatttatc gaagcacggg gagacgcacc gcatgctata tattccgact
      481 cttccctggt ctttaaaaca atgtccagaa catagttttt aatattgtca tcaatgtaaa
      541 tgccgtcaac cttttttctg agcctgaaaa tgtcttcgcg ggacaatacc ttcctaacat
      601 ccggatcggc aggttgttct ttggagtaga agtcaataat tctgctttct tcatcccggc
      661 cgggatactt tatcttcagc ttcatcataa aacggtcctg ctgtgcttcg ggaagcggat
      721 aagttccctg ctgctcaaga gggttctgcg tagctataac caaaaaggga ttgtccaggt
      781 gataagtttt ttcaccaatg gttacctgtt tttcctgcat tgcttcaagc agggctgact
      841 gcacttttgc aggtgcacgg tttatttcat ccgcaagaat cagattgctg aaaatcggcc
      901 ccttctttac tataaactct cctgtctttt ggttatatat ttcggtgcca agaatatccg
      961 ccggcagcaa atccggcgta aattgtatcc ttttaaaatc aatccccaca acctgggcta
     1021 tggtattggc tgttaacgat ttggccaaac ccggaacacc ctcgagcaaa atatgtcccc
     1081 ctgtcaaaag cccgatcaat attctgtcaa ccatatattt ttgtccgaca attatctttc
     1141 ctatttcttc cttcaggtca gttaatatct ttatttcatc aagaaattct gttgtttcca
     1201 taggttaacc tccgcaatat actttttaaa caggatacaa tacttccctg tacaagcaat
     1261 ttatatttta aacataaagt tttacgtatt tgtgaacaat ctatgaactt tggaggcatt
     1321 aaataaatgg gttatttgtt tatacagata taagccacaa gcaacagccc cggaattaaa
     1381 ggaataaact caattataga aacccgtgtt tgattttcac gaggtttttc aacatttttt
     1441 gaattaagct taatgaacca atacaccaaa actccaaaaa cagcagccga aaatgatact
     1501 actgccaatg cgacggctgc cataaagaca atctccggaa ttgacatgcc ctgaaactga
     1561 gaaaatacat cctgtccggc agggttattg acgttctcta ctcccatttt ctccaaaaac
     1621 tcaagcattt ttatactaac ccagttcaat accaccgcaa cagcattatt tgtaaagtgg
     1681 gcaaacattc caatcatcag ggaatcactt ttgtatacca gaaaacctat cagtgccccc
     1741 aaaagaaatg ttccaaacag actttgaaag ctaaagtgcg tgagtccaaa aagaaaagcg
     1801 gtaatcaaaa tggatttgac gcttccgaat ctctcaaagc tcctttgaat tactcctctg
     1861 aaaagcactt cctcacatat tcctgccgaa attgcgatga ccagtacgcc gataaaaaga
     1921 tccccaacac cgcttcctat gggaatttgt gttgtttcca cggttccgga caaaagcttt
     1981 actataagaa tattcaataa attgaacaca cccacaatcg gaatgccgaa tatcacaatc
     2041 ccaaaagcca aaacaaagtt tacaaaactt gtcctgttcc acctcagtac ctttttggta
     2101 tcaaacctga atataaacag aaataccaga ggaggaagca ttattaaaaa tacctcggaa
     2161 atcaggcctc caatgttaaa ttgtaaagcc tcctgcagtt ttgtggatac aaacacaagc
     2221 agtatcacaa ccagggaata caaaagtccc acctgccata tcctcggttt ttccacgtca
     2281 tattgttcct gtacttctac actgtgttca tccatgaatt ttcccccttt ttatgatgat
     2341 ccggatatgt ttgtcatgac tgaaagaata tgattatatt ataacacttc aattcaaaga
     2401 agattttcgt ttacagaaaa ttttcttgca tctctctcca aattaatatc cataataggg
     2461 ctgcatattg tttatgtgaa gtaaaatatt gaagaaacat ataaaagcaa gttttagagc
     2521 ctgttaatac gacgtggaaa ggggagcatc cattatcacg aagagagttt ttttagaccc
     2581 tcacggagta tttacaggct cttaacctgt ggaacttaag tttttcgagt acttctcttc
     2641 acaaccaatt aatgcataca ttacgttaga accaccagta gctacattgt cagtatgcca
     2701 ttgggtgtgt ccataatcag caatatattc tcctctttac cgttctcggc attaatataa
     2761 actaaaaaat ccctgtcatt gagcttaccc ttaaactcat atgtgaatat ctccgtctta
     2821 tagtccgtag gtattatagc aagaccgctg cttaaaacat tcattcttgg tgtaataagt
     2881 gcacgggcct gttccattgt aatcttcggc tgaggtatgt ttctttgcgt atggttggtt
     2941 atatagccct tgctctcaaa gccgacaatt tcgccgttat ccaatgctat tttcaccttg
     3001 ataaggtcgg ggtaaacagt tacatccccc tgtttatatg cataattaat aattgcggta
     3061 ttatcctctt tcaagtaata ggtatccacc atgtccttat atccatgctc ttccaagaac
     3121 tttttcccca aagcctttgc ctggtctacg tttatctgtt cctgggcacc taccggtctg
     3181 ttatacagca tccacaagac atttccgccc ttttgggtta catccacagt cgcagtctga
     3241 tcctccggaa cattattgaa tttaacacta tagctgtatg tcttgatatt cccttcatcg
     3301 actctgccaa tttcctcaac agccttcacc ttatccggtc cgaaaaactc cataaccctt
     3361 tgttttgcct gttcgacatc catgtcctct cccgcaagac ccttaggttc tgtctgtgtc
     3421 atgtggtcgg aaaaaggacc gtcataaatc aatgtgggat attcctgaaa agttttttct
     3481 atgttttcaa actgggcatt gggcatattc tccgaagtct tctgaaacaa acgcgtcccc
     3541 ttgtttgcca aatcacccca ctttattctt cctgtcgtaa tctggttttg aaggtcgatt
     3601 aaactctgtt ccaatgaaac cgaaaaccca tgcagctgct caataagctt gtactgttct
     3661 tcatttaaac ccttccctcc catgttctgg ttgtttaaag aatacgccaa gtcacccacc
     3721 tgggtaagga attttgatgt gttggccagt acattaggag acaccggaag ctgccccaga
     3781 ttggtctgtg ccaaatttgc ctgtctccat gcctcttgca tggttgctgc tgtcttttcc
     3841 ggggtattgg cgataaggga ttttatcagc agcacctcga cattgttgac ataccccacc
     3901 atatcgtaaa acgcacggtt gtattggtta tcaagctcct gccttaaatc agcagcatgc
     3961 ttgtattggt agaaccccca catagccaca gcagcaattg caacaattac tatgctgtac
     4021 atttttctat cggatagtct tctttttaaa tccagtaatt tttctcttat actcaaaaag
     4081 ctgcacctcc taaattattt gccgaaataa tgctttccta tttttttaac gatttgcctg
     4141 ctccaaatcc acttgctggt tgcagtggca ggattccaat aatataagca cccgttggtt
     4201 ggatcccaac cgttcagtgc atccctggct gccttaacaa cggttgaatt cgggtctatg
     4261 ggtacattta tctgcccatc ggataccgca gtaaaggctc cgggttgata aatcacacct
     4321 gccacagttt tgggaaactt tggatcccgc gtccggttaa gtataaccgc acctactgcc
     4381 acctggccct catagggctc tccccttgcc tctccgtata tagccctggc gagaagctgc
     4441 tcattgctag tctgattcga ctgggcatac acctcacccc attttgccaa cgtcgtaagt
     4501 cccatggctg tcaacgtatc ctcatttgca ataccgctta caggcaggtt atacgtcttt
     4561 tgaaaatccc ttacggcaaa atatgttttc aagtcatatt tcccactaat ctctccgtca
     4621 tagtagttcc aatttttcag cctttgctgc atcttcctga catcatcgcc ttccatatcc
     4681 ggtgccagct taccggtaat tggagcgata aagaccgaac cttttaaaaa caggggtatg
     4741 gaaacagaaa atataacaac aagaataatt atccttttga ccgttttcaa ataatcactc
     4801 cctctttata aatacttgat taaagccaat ataacaaaag attaataatt tagcattgtt
     4861 atatactcaa aagttatttt ttgttttatt gaaaaaaata ttcgttggtt ccattattat
     4921 aggtgcatat ataatgtgaa gaaaaatgtg gaagaaacat tgtgaagcaa gttgtagagc
     4981 ctgttaatac gacgtggaaa gggagagcat tgtctgaacg aagtgagttt tgctcgaccc
     5041 cacggagtat ttacaggctc tgtgcttgcg gaactcaagt ttctggaaca tttttcttca
     5101 cataagcaac atacgcaatt aagcaacatg cacaataggc atataaaata aatgaagaaa
     5161 atacatacca cgattttatg tataactctt cactatcttc caaataataa atcagggaaa
     5221 attctattgt cagaggttga aaatgaggat tttgtttttg tcgatttcaa tgggagcagg
     5281 acatataaag gctgctgaag cactaaaaga atatatagtc cttcgctatc ccggttcgga
     5341 aaatttaata gttgatacct taaaatacat aaaccctgtg gttcacaaag ttgttgtaga
     5401 cggatacctt aatgttataa agaaaaaacc cgaaatttac ggatgccttt acaggatgtc
     5461 cgagtacagg gaaaacataa ccagattgag ccaaagagta agtagtttat tggcatctaa
     5521 aattcacaaa ctggtgctgg actttaaacc ggcaataatt gtgtgtaccc acccctttcc
     5581 ccttcagatg gtggcccact taaagaaaca caaaaatatg aaggttcctg ccgttgcaat
     5641 tttaacagac tttgtaaacc accccctgtg gcttcatgac aacatcgaag cctatattgt
     5701 ggggcacgat tacattaaac atgacatgga ggagtgcgga attgacaaaa attgcatctt
     5761 ctcttacggg atccccgttt cccgtaagtt tctaaagccc acgtccaaag ccacggcacg
     5821 aaacaggctt tctttagaaa ataaactgac ggtactcctt atgggtggaa gccttggaat
     5881 aggagaaatc gaaagcagct ttcgagcctt tgcagggtgt aagcgggacc ttcaaataat
     5941 tacagtggca ggcaaaaatg ctgtgcttga agaaaaaata aaagccctgt caaaatcata
     6001 taacaagccc ataaggacct tggggtacac acaaaacatc cccgagctta tggactgctc
     6061 ggatttcata gctacaaaac ccggaggaat gaccatatcg gaagcccttg taaaaaagct
     6121 tcccatcctt attatctctc caataccggg acaggaggaa agaaacgccc gctttcttat
     6181 aaacagcggt gctgcggtag ctataaacaa gaaggacagt attgacggaa cactatgcca
     6241 ggtttttgac aatcccctta gataccgcca catgagtcaa atggccgaaa acctttcaac
     6301 gcccgaagcc ggccataata ttgtaaatct tatggaaaaa cttatacatg acaatctatt
     6361 caaatattct tctaataccc tttagcctct ttttatagta ttccgagttg atactgccat
     6421 aggttacttt tcctgccgat tggctggcat gtataaactg atcctcaccc aaataaattc
     6481 ccacatggga aacattggca agatcttcgt tagagctgaa aaacagcaag tccccgggtt
     6541 ttaacccacc ggtattctgc ggtccggttt taataaaatc atactgcata tccgcatccc
     6601 tcgggagatt aacaccgttt attctgctgc aaatgtacac aagtccggaa cagtcaagcc
     6661 cttgccatgc actgacgcca ccccaaaggt actgtgtttc cataaacttt ttcaccgttg
     6721 ctaaaaaatc ttgggcggat gttttgggta ttgggctctc cacaggaacc tttattgtgc
     6781 ctttggtttc aatccatccg gtaatatttc ccggcagtac aacctcaaag tacctatcct
     6841 ttttattttt aatataaagc tccgtcccca tcacgacgtc ctttaaagta acaccgcctc
     6901 cggcaccgga ataaaccttc tgggtctttt cggtgacaac agccttgtcg gtaaaatttt
     6961 ccttcatgat acttgagcag tccctgtcaa tgtattttga cctcaaccag cctgtatatc
     7021 catccactac ttttaccttt acccacgaat ccttttcttc caatatggta acaggctgat
     7081 tgaaaatagc ctgtgtgacc cgttcggagg ttatatcagg ctctctgaat acatccacca
     7141 ccgtatccag cacaaccgct acattgtcgt agctaacccc ttcggaacct ccctttattt
     7201 tatcaccttc atcggatgga atagttgtat cctcaccctt accgtgtgta gaaatggctt
     7261 ctccgccggc agtcacctct ggagaaggta tttcaattgt attgtcaaca ggctgatcac
     7321 agctgcaaag aaccatgtaa agcatcgtta tattcaataa taaaatcaat gtccttttca
     7381 tcaaaaaaac ctca
//
LOCUS       DAOOJC010000063         7363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_8473_length_7363_cov_3.352080, whole genome shotgun sequence.
ACCESSION   DAOOJC010000063 DAOOJC010000000
VERSION     DAOOJC010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8473_length_7363_cov_3.352080"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1876)
                     /gene="carB"
                     /locus_tag="PLA01_02915"
     CDS             complement(<1..1876)
                     /gene="carB"
                     /locus_tag="PLA01_02915"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HPD00284.1"
                     /translation="MPKREDVKKVLVIGSGPIVIGQAAEFDYAGTQACRALKEENIEV
                     VLVNSNPATIMTDTNIADRVYIEPLTVEVVKNIIRKEKPDSILPTLGGQTGLNLAMEL
                     AESGFLQENNVKLLGTATEAIKMAEDRQAFKDTMERIGEPCIASKVVNTIEDALEFAK
                     EIGYPVIVRPAYTLGGTGGGIVNNEEELLEIGSNGLRLSRVHQVLIEKCISGWKEIEY
                     EVMRDGRGNVITICSMENIDPVGVHTGDSIVVAPCQTLSDKEYQMLRSASLKIITALG
                     IEGGCNVQFALNPNSFEYAVIEVNPRVSRSSALASKATGYPIAKVASKIAVGYSLDEI
                     KNAVTGKTYACFEPTIDYVVIKIPKWPFDKFVKASRTLGTQMKATGEVMAISSSFEGG
                     IMKAIRSLELGIFTLEQDVYKKFSDEEIRNKIKDINDERLLVIAEAIRRGISLEDIND
                     ITKIDLFFLNKIKNLVLTEEKLKTMSLSDFTADYLRKVKKLGFTDGIIAKYVGCSKEE
                     VRAKRKELGVCAVFKMVDTCAAEFEAVTPYYYSTYDEHCEAKKSDKKKVLVIGSGPIR
                     IGQGIEFDYCSVHSVWGLKDEGYETIIANNNPETVSTDFDTADRLYFEPLTEEDVENI
                     V"
     gene            complement(1949..3025)
                     /locus_tag="PLA01_02920"
     CDS             complement(1949..3025)
                     /locus_tag="PLA01_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HPD00285.1"
                     /translation="MRAVLALEDGTVFYGEGFGVLGEVIGEIVFNTGMTGYQEVLTDP
                     SYCGQIVAMTYPLIGNYGANDDDIESIKPQVKGFIVRELCNTPSNWRNKETLDDYLKR
                     YNIIGIQGIDTRALTRILREKGTMKGIISSDPEFNLERKMEEVKSYVIKNPVDRVTTE
                     KVLHYSGNGRRVSLIDLGMKQNIIRSLQRRGCDVHVFPANSKAEDILSVNPDGIMLSN
                     GPGDPKDCTDTIKNIKELMGKRPMFGICLGHQLIALANNADTQKLKYGHRGCNHPVKD
                     IEKNLTYITSQNHGYTIVESSLDEKRIKVTHRNMNDGTVEGVRYKDMPVFTVQFHPEA
                     SPGPTDTEYLFDEFMEMMEVQRKI"
     gene            complement(3052..3999)
                     /gene="pyrF"
                     /locus_tag="PLA01_02925"
     CDS             complement(3052..3999)
                     /gene="pyrF"
                     /locus_tag="PLA01_02925"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPD00286.1"
                     /translation="MFVDTLIDKIKEKNNPSVVGLDPKIEYVPSFIKEEMFEKYGKNL
                     KAVSEAILTFNKYIIDAVYDIVPAIKPQLAYYEMYGLEGMRVFAETCKYAKQKGLLVI
                     ADGKRNDIGSTAEAYSGAYLGRTLIDEGISEPIFDVDALTVNPFLGIDGIKPFISDCI
                     NYGKGIFVIVKTSNKSSGQFQDVLTMESKSMYEIVARYVEEWGQYVKGKYGYSSVGAV
                     VGATYPNQAKILRKILKSSYILVPGYGAQGGTARDVAHSFNYDGLGAIVNASRSIMCA
                     YKSEQWKDTYSEERFYEASRAEAIRMRDDINNAVNGSKT"
     gene            complement(4150..5443)
                     /locus_tag="PLA01_02930"
                     /pseudo
     CDS             complement(4150..5443)
                     /locus_tag="PLA01_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924163.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
     gene            complement(5427..6365)
                     /locus_tag="PLA01_02935"
     CDS             complement(5427..6365)
                     /locus_tag="PLA01_02935"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPD00287.1"
                     /translation="MTLKSKDLLGLKDLTEEEIEYILRTAKTMKLILESKNKKTPHLQ
                     GKSVITLFYENSTRTRLSFELAAKYMSASAANISVAASSVAKGETLIDTGKTIDMMGA
                     DVIVIRHSMAGTPHLLAKNVKASVINAGDGMNEHPTQALLDMFTVVEKKGSLKGLKVA
                     IIGDIYHSRVVRSNIWGMTKLGAEVCVAGPTTLIPRELDKVGVKVFNTVQEALIDADV
                     VMGLRIQKERQKKGLFPSLREYYRFFGLDDKRLKLAKEDALVLHPGPVNRGVELSSAV
                     IDGDKSLINEQVTNGVAVRMALLYLLTRRDNVEGSN"
     gene            complement(6648..7202)
                     /gene="pyrR"
                     /locus_tag="PLA01_02940"
     CDS             complement(6648..7202)
                     /gene="pyrR"
                     /locus_tag="PLA01_02940"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPD00288.1"
                     /translation="MVDKAVIMDESGIFRAATRISHEIIEKNKGVEDLVLIGIQRRGV
                     PLAKMISEKIKGVEGKEVPVGILDITFYRDDLSMLSEHPIINATEINFPINGKKVVLV
                     DDVIYTGRTARAAIDALMDIGRPKLIQLAVLVDRGHRELPIRPDYVGKNVPTSKFEVV
                     NVKLNEIDGENAVTISDLEKEISH"
BASE COUNT         1922 a         1893 c         1156 g         2392 t
ORIGIN      
        1 caactatatt ttccacatct tcttcggtca acggctcaaa atacagtctg tcggcggtgt
       61 caaaatccgt actgaccgtt tccggattgt tgttggctat tatggtttca taaccctcgt
      121 ctttaaggcc ccataccgaa tggacggaac agtagtcaaa ctcaattccc tgaccgattc
      181 ttataggacc ggatccgatt accagaacct tctttttatc agattttttt gcttcacagt
      241 gttcgtcata ggttgagtaa taatacggtg ttacagcctc aaactctgcg gcacaggtgt
      301 caaccatttt aaacaccgca caaacaccaa gttcctttcg tttagccctg acttcctcct
      361 tgctgcatcc cacgtacttt gcaatgattc catcggtaaa gcctaatttc tttacctttc
      421 tcaaataatc cgctgtgaaa tccgaaaggc tcatggtttt aagcttctct tcggtaagaa
      481 ccaggttctt gattttgttt aggaaaaaca agtcaatctt ggtgatatcg ttaatatcct
      541 ctaaagaaat gccccttctt atagcctcgg ctataacaag aagcctttca tcatttatat
      601 cctttatctt atttcttatc tcctcatcgc tgaatttctt gtagacatcc tgctccaatg
      661 tgaaaatacc cagttccaaa gacctgatgg ccttcatgat cccaccttca aaggaactgc
      721 ttattgccat tacctctccg gtagccttca tctgggttcc caaggtcctg ctggccttta
      781 caaatttgtc aaaaggccat tttggtattt ttataacaac atagtcgatg gttggctcaa
      841 aacaagcata ggtcttacct gtaactgcgt tctttatttc atccaggctg taacctacag
      901 caattttgga tgccaccttc gcaatcggat agcccgtcgc tttcgaggcc aaggccgatg
      961 aacggctaac ccttgggtta acttcgatta ctgcatattc aaagctattg ggatttaagg
     1021 caaactggac attacatccg ccttctattc ccaaagcggt tattatcttg agcgatgctg
     1081 aacgaagcat ttggtattcc ttgtcggaaa gggtctggca tggtgcgaca actatactgt
     1141 ctccggtatg gacacctaca ggatcgatat tttccatgct gcatatggta ataacatttc
     1201 ctctgccgtc ccgcataacc tcatattcta tttccttcca tccggatata cacttttcta
     1261 tcaatacctg atgtaccctg ctcagtctca acccattgct tccaatttcc aaaagctctt
     1321 cttcgttatt tactataccg ccaccggttc ctcccaaagt ataagcaggc cgtacaatta
     1381 ccggatagcc tatttctttt gcaaactcca gagcatcctc aatcgtattt accaccttgc
     1441 tggcaataca gggctcccct attctctcca ttgtatcctt aaaagcctgc ctgtcttctg
     1501 ccatttttat tgcttcggtt gccgtaccca aaagctttac attgttctcc tgcaagaacc
     1561 ccgactcggc aagctccatg gcaaggttca aaccggtctg tccacccaat gtgggaagta
     1621 tgctgtccgg tttctctttt cttattatgt ttttaaccac ctctacggta agcggctcaa
     1681 tataaaccct gtccgcaata ttagtgtctg tcataattgt agccggattg ctgttaacca
     1741 atacaacttc tatattttct tcctttaaag ccctgcaggc ctgagttccg gcatagtcaa
     1801 actctgctgc ctggcctatt acaataggac ccgagccgat aaccaaaacc ttcttaacgt
     1861 cttctctttt aggcatttaa catcccccca atactattcc ttctaaaatg tccactgttt
     1921 tatataaatt cctgtagtac tctcctaact aaatctttct ttgtacttcc atcatttcca
     1981 taaactcatc aaaaaggtac tccgtatccg tcggtcccgg tgatgcctcc ggatggaact
     2041 gaaccgtgaa caccggcatg tctttgtatc gaaccccttc aacggtgccg tcattcatat
     2101 tcctgtgagt cacttttatc cttttttcgt ccaatgacga ttctactata gtataaccat
     2161 gattttgaga agtaatatat gtcaggtttt tttcaatatc cttcaccgga tgattgcacc
     2221 ctctgtgacc gtatttcagc ttttgcgtat cggcattgtt cgccagggca ataagctgat
     2281 ggcccaggca aattccaaac ataggccttt tgcccataag ctcctttata tttttaattg
     2341 tatccgtaca atccttcggg tcgccaggtc cgttagaaag cattattcca tcgggattaa
     2401 cggacagtat atcctccgcc ttcgaatttg cgggaaaaac atggacgtca cagcctcttc
     2461 tttgaagaga acggataata ttttgcttca ttcccaaatc tatcaaggat actcttcttc
     2521 cgttaccgct ataatgcaaa accttttctg ttgtaaccct atccacaggg ttctttatta
     2581 cataagactt aacctcttcc atctttcttt caaggttaaa ttcgggatcc gaactgatta
     2641 tgcccttcat cgtacccttt tcccttaaaa tccttgtaag agctcttgta tcaattccct
     2701 gtatccctat aatgttatac cttttcaaat aatcatccaa tgtctcttta tttctccaat
     2761 tacttggagt attgcatagt tccctgacaa taaaaccttt tacctggggt tttattgatt
     2821 caatatcatc gtcgttcgcc ccatagtttc ctatcagcgg ataagtcata gccacaatct
     2881 gtccgcaata ggaagggtcc gtcagaacct cctgatatcc cgtcattccg gtattaaaga
     2941 ctatttctcc gattacttca cccaaaactc caaaaccttc tccataaaaa accgtaccat
     3001 cttcaagggc caaaaccgct ctcataatct atcccacctt aatttttaaa attatgtttt
     3061 agaaccgtta actgcattgt ttatgtcatc tctcatccta attgcctcag cccgggatgc
     3121 ttcataaaat ctctcctcgc tgtaagtatc cttccactgt tcagatttat aggcacacat
     3181 tatacttctt gatgcattta ctattgctcc caaaccgtcg taattaaagg agtgtgccac
     3241 atctctggct gttccgccct gggcaccata tcctggaacc agtatataag aactctttaa
     3301 aatctttctc agtattttcg cctgattggg atatgttgct cctactactg ccccaacact
     3361 gctgtatcca tacttacctt taacatattg tccccattcc tcaacatacc gggcaacaat
     3421 ttcatacata ctcttgcttt ccatggttaa aacatcctga aattgtcctg atgacttgtt
     3481 ggaagtcttt actattacaa atatcccttt gccgtaattt atacaatcgg atataaaagg
     3541 ctttattccg tctatgccca aaaacggatt gaccgtcagt gcatccacat caaaaatagg
     3601 ctcgctgatt ccttcgtcta tgagtgttct tccgagataa gctccggaat aagcttctgc
     3661 tgttgaaccg atgtcgtttc tctttccgtc tgcgataacc aaaagccctt tttgcttagc
     3721 atatttgcag gtttcagcaa ataccctcat tccctccaac ccgtacattt cgtagtatgc
     3781 caattgtggc tttatagcag gcacaatatc gtacactgca tcaataatat acttattgaa
     3841 ggttaaaatg gcttcggata ccgcttttaa attcttaccg tatttttcaa acatctcttc
     3901 ttttataaaa gaaggcacat actctatttt aggatccaat cccactactg aagggttatt
     3961 tttttcctta attttatcaa tcagtgtatc aacaaacata atccaatctc cttattttac
     4021 ataatattgt atttatatat aaactgcaaa gtaatttgct tccatcaggt atcgaactta
     4081 acttcattga aatccttcca accctacttc agcaatctat cttttacagt gtgtttttta
     4141 aactgaactt caaagaagta ccttttctct gacaacaaca ttgccgttta ctatcgtata
     4201 ataaaccgtc cccttaagct tgaatccgtc aaagggcgaa tttcttgcct ttgatttaaa
     4261 cttacctaca tccacaacaa actcttcttc aatgtctact attgttatgt ccgcactcct
     4321 gcctatttcc agagaacctt tgttgagtcc cagtattttt gacgggttca cgcacatctt
     4381 ttccaccagt tgattcattg taaggtgtcc cggttttaca agatatgtta ctgccagagg
     4441 caaggctgtc tcaaatcctg cgataccgtt tgccgccaga ttgaactcta catttttctc
     4501 gtccacatga tggggtgcgt ggtcagtggc aattatcaat ggtcccatct ttcagacctt
     4561 caattaccgc ctcaacatcc ttttgggttc taagcggagg attaaccttg gcatgggtat
     4621 tgaaacccga acacgcttca tccgtaagca caaagtagtg gggacatgtc tcacatgtca
     4681 ccttgacccc tctctttttg gcatgcctta tcaagtcaac agccaattct gtactgacat
     4741 gggctatatg aatcggaacc ttcgtatatt ccgctaatat aagctctctt gccaccatta
     4801 tctcttctgc cgcagagggt atacccttaa gccccattat tgtagaccag tatccctcgt
     4861 tcatcacacc ctcatctgca atgtccaaat cctcacagtg tgaaatcacg gcaatatcaa
     4921 acattgatga gtactcaaga gcccttttca tgagcgaaga actctttagc ggttttccgt
     4981 cgtccgatat ggcaacagca cctgcaaact tcaattcacc gatttccgaa agttcttcac
     5041 ctttttgacc ctttgaaatg gcaccaatgg ggtaaacatt gaccactccg tcctgttttg
     5101 ctttatttat aatgtatttt ataaccgact cattgtctgc aacagggttt gtgttcggca
     5161 tacaagctac ggatgtgaat cctcccgctg cagcactttt tgttccgctt tcaatgtctt
     5221 ccttgtattc aaaacccgga tccctcaagt gacaatgggc atccaccaga ccgggcagca
     5281 ccaatttgcc tccagcatca atcacatcac aatttaaagc ctccaggtcc tcgtcaatat
     5341 tgccaatctc cgttattatc ccatcctcga tcaatatatc cgttatactg tccgtattgg
     5401 ttttaggatc tataacatgt ccacctttaa ttactaccct caacattatc cctcctcgtc
     5461 aaaagataaa gaagagccat tcttactgct actccgtttg taacctgttc atttatcaga
     5521 gacttatcac cgtcgattac cgccgacgat agctcaacac ctctgttaac cggcccggga
     5581 tgcaacacca atgcatcttc ttttgcaagc tttaaccgct tgtcatccag tccaaaaaac
     5641 ctgtaatact ctcttaagct cggaaacaag cctttctttt gtctttcctt ctgaatcctt
     5701 agtcccataa ccacatcggc atcaataagt gcctcctgaa ccgtgttaaa gaccttcaca
     5761 ccaactttat ccagttcccg gggtattaag gtagtgggtc cggcaacgca cacctcggca
     5821 cccaattttg tcatccccca gatattgctc cgaaccactc tgctgtgata aatatcacct
     5881 attatcgcaa cctttaagcc ctttaagctt cccttctttt cgacaacggt aaacatgtca
     5941 agaagtgcct gggtcgggtg ctcattcatc ccatcaccgg cattaatcac tgaagccttt
     6001 acatttttag ctagcaaatg cggagttccg gccattgaat gccttattac aattacatct
     6061 gcccccatca tatcaattgt ttttccggta tcgataagtg tctcaccttt tgccacactg
     6121 cttgcggcaa ccgagatatt cgcagcactg gcactcatgt atttggcagc cagttcaaag
     6181 gacagtctcg tcctggtgct gttctcgtaa aacagagtga taaccgattt accctgaaga
     6241 tgaggagtct tcttattctt tgattcaaga ataagcttca ttgtttttgc agtgcgtaaa
     6301 atgtactcga tttcttcttc tgtcaagtct ttaagtccca ggagatcttt tgattttagt
     6361 gtcatcgcta caccccattc ttaataaaac ttattataaa aagcccttat accaaagacc
     6421 ctattacagt gcacataaaa atagtaagga gaaaatctcc ttactatctg aaaaaacact
     6481 tatgctatta aaaacactta tatctttaac gaaaacaacc tcaggtttaa aaatggaaat
     6541 aatactacaa gtcttaattc tttcctttag tttttcaaaa tagacatgta ccatacttct
     6601 cattttaaac cctctccaaa aattgtttat ctattaatca tacaaagcta atggcttatt
     6661 tctttttcca agtcgctgat tgtaaccgcg ttctctccgt caatctcatt aagcttgaca
     6721 ttcactacct caaactttga tgtcggaacg tttttaccga cgtaatccgg acgtatgggc
     6781 aactctctgt gtcccctgtc gaccaatact gccaattgaa tcaactttgg ccttcctatg
     6841 tccatcaatg catcaatagc cgcccgtgca gttcttcccg tataaataac gtcatcaaca
     6901 agcactactt tcttaccgtt tataggaaag tttatttctg tagcgtttat tatcgggtgc
     6961 tccgaaagca tgcttaaatc gtctctataa aaggttatat caagaatgcc aacaggaacc
     7021 tcttttcctt caactccctt aatcttctcg gaaatcattt ttgcaagggg cacgccccgc
     7081 ctttgaattc caatcagtac aaggtcctcc acacccttat ttttctcaat aatttcatgg
     7141 gatattcttg ttgcagccct aaatattcca ctctcatcca ttattactgc tttatcaacc
     7201 attttattta ctctccgttt tgtttttggt tatttcactt tttgaaatcc cactaaaaaa
     7261 gctccttacc acaaggtaag aagccaataa atgtatagtt tatttgccag tcaaaataca
     7321 tattaataca tcttaccttt tgagtctctc aggacttaat taa
//
LOCUS       DAOOJC010000064         7290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_8576_length_7290_cov_3.274361, whole genome shotgun sequence.
ACCESSION   DAOOJC010000064 DAOOJC010000000
VERSION     DAOOJC010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8576_length_7290_cov_3.274361"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PLA01_02945"
     CDS             <1..712
                     /locus_tag="PLA01_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HPD00289.1"
                     /translation="YGVIYTLREALKALGIDIASTTASLQGFGNVAEYAARLYTKMGG
                     KIIAISCWDNQDQKAYTFRSLKGLDIEKMVSIKDTFGTIDKKKAQEMGIELLDGGEWI
                     SQDVDILLPCALENQITPDTLAKISNQVKVICEGANGPTTPDSDEIIKAKNIYLIPDF
                     LCNAGGVTCSYFEQVQCNMNYFWPKEEVLEKLDQKMTSAFHAVHKLSQEKKLYMRDAA
                     YVIAINRVAEAVKLRGWV"
     gene            858..3986
                     /locus_tag="PLA01_02950"
     CDS             858..3986
                     /locus_tag="PLA01_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPD00290.1"
                     /translation="MTQFKKVSKDDLVERAKELECLYLVDEALTELSLSDSLEKITSI
                     IPLGFRIPHNCTVVIELDGQVYNQKASVNNTDELCSDITVNGKSRGYIKVLYPKQTFP
                     ENDVVFLEQESRLLNTIAKRISETIDKRDSVKKFNYRNRWEAILDLLQKTDHEILLYV
                     CEKMLSLLSRINPPLVQNVFTEMNWSKYDTHGEINFPLETLPEVNIIHLSRILFENST
                     KILEDSQIYDYINLWVYQGKTYELIKIVDKRDSDIKKISQALSAYVKAVKNNKMSSET
                     TRRWLRVELTRRFLTDNPKTISKIHNYINVEAFIKLLDTFICSPRSIGRIGGKGSGFF
                     LANQIIETYKKEFPELENIIAPKTWYISSDEFKYFLEDNGFEELNEHKYSDITDVRTS
                     YPRIIQMLKNARLSPYILNELNQVLDACEDCPLIIRSSSLLEDQIDSSFSGKYKSLFI
                     TNTGTRAERMKQLVDGILEVYASIFSADSIQYRKERNLLDCNDQMGILIQEVVGCKVG
                     RYYFPLFAGVAFSNNELRWSPRIKREDGLMRMVMGLGTRAVDRVVEDYPILLSPGQAN
                     LRVNQSPYELLKYSPQYIDVIDLENNRFLTLPISDLIKECGDQIPNINLLASVLKNDI
                     VMDANPLTTDFKTDKIIITFDGFIKKTPLVKQVKSILSLLQTELGYPVDIEFASDGKY
                     FYLLQCRPQSRNHDNAPVAIPANIPAQCTVFTASKYVSNGKVTGIKTVVYVDPNEYSR
                     LESHEDLLDIRKIISELNRLLPRRSFILIGPGRWGSRGDIKLGVPVAYSDINNTAMLI
                     EVAQKQSKYEPELSFGTHFFQDLVEENIKYLPLYPEDNDIIFNQAFFRGSKNTLPDIL
                     PMYSHLCDVVKVINIEETFFDKELIVLMNGDLQKAIAYLEQPSRVNSESNSDFTSAPV
                     TNDYDEEGWKWRHYMAEQIAHQVDMDAFSVKGIYLFGSTNNCTARLNSDIDLLIHFDG
                     NEEQKKSLNTWLQGWSMALAEINYLKTGYKSSGLLDIHYITDDDIKNRTSYAIKIDSV
                     LDPACPLRIRAK"
     gene            complement(4110..6461)
                     /locus_tag="PLA01_02955"
     CDS             complement(4110..6461)
                     /locus_tag="PLA01_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPD00291.1"
                     /translation="MLKKFSAKMSLLVVCTILISVIAATATTLNVQAVLSFELIGFAT
                     LNGGTTGGTGGTEITATSVSQVNDILSARKKNKDTSPLIIKFDRKLTGSEVIACKEVS
                     NITFLGVGDKGELEGCGINIVKSKNIIVQNLKIHHTRAPMDAIGVENSQNIWVDHCEL
                     YNMIGDCNGDGIVNPNDGDTEGGDVDWYDGLLDIKKSSEYITVSWNYFHDSYKTSLIG
                     SSDSDDYDRKITFHHNVYSNVKSRTPSYRSGTGHMFNNYYVNVSGSGINSRVGAKLRI
                     EGNVFENAGCGSVDSKTGFAEGPIGSYYSSNIGYWDVRDNIFVDCKGNQPTTSTCSFN
                     PPYDYSRVLQPASQVKETVLQYAGVQGNSIPTPSIPPQTSSPKTTPPQITPTPTTYHP
                     EIVYGDINGDGLVDSTDLTILKRYILRRLDFTSDQLKAADVNLDNEVNSTDITILKRF
                     ILRKIDSLPVNNGNQPTPTPTSASTPTPTPYTSRPTTPPPTSTPTEGDIILEPNGSMD
                     LQQAINAIKPGQTIYLKGGNYNYSKTIVIAEGNDGTSSAMKRIVAYGSDKPVIDFSAM
                     SENSSNRGAVLAANYWHIKGITIKGAGDNGMLLAGSNNIIEACTFTENHDSGLQLSRY
                     NTSYKSIDQWPSNNLIVDCISTLNFDSGREDADGFAPKLTCGRGNVFRNCKALYNCDD
                     GWDMYTKSDTGPIGVVTLENCEAIGNGYDTNGKKTGGDGNGYKLGDDTASVPHVLINC
                     VAKGNAKHGFTGNGNPAKIVLENCTGSGNAQKLFDRLDNAIFK"
     gene            7080..>7290
                     /locus_tag="PLA01_02960"
     CDS             7080..>7290
                     /locus_tag="PLA01_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00292.1"
                     /translation="MKKKYIFLLISIEIILIALLIYFFVPKSGLNTHVEAVNMNLTNY
                     KYDYYVQRNLIKIAGDVGTASYVFKG"
BASE COUNT         2156 a         1438 c         1390 g         2306 t
ORIGIN      
        1 atatggtgtc atctatacat taagggaagc tcttaaggca ttgggtatag atattgcttc
       61 tactacagca agtctgcagg ggtttggaaa tgttgccgaa tatgctgccc ggttatacac
      121 taaaatggga ggcaaaatta ttgcaatttc ctgttgggat aatcaagacc aaaaggctta
      181 tacattccgc agtctcaaag gtcttgacat tgagaagatg gtttccatta aggatacttt
      241 cggtacaatc gacaagaaaa aagcacagga gatgggtatt gaattattag acggcggaga
      301 atggatctca caggacgtag atattctttt gccttgtgca ttggaaaatc aaattacgcc
      361 ggataccctt gctaaaataa gcaatcaggt gaaagtaatc tgcgaaggtg ccaacggacc
      421 tactaccccg gacagtgatg agatcataaa ggctaaaaac atttatctta ttcccgactt
      481 cctttgcaat gccggaggag ttacctgcag ttactttgaa caggttcaat gcaatatgaa
      541 ctatttctgg ccgaaagaag aagtattgga gaaactcgat caaaaaatga ccagtgcttt
      601 ccatgccgta cataagttat cccaagagaa gaagctgtat atgcgtgatg ctgcatacgt
      661 aatagctata aaccgtgttg ctgaggctgt taagctgcgg ggttgggtat aataatcctg
      721 gtttcgtttt tataaataac attaagttgt atggtttcgc gaaaatataa ttgaatgtaa
      781 aaatattttt cgcgaaatca tataactaat gagttttttt aaattaccct caatctaaga
      841 agggcaggta aaccagcatg acacagttta aaaaggtatc gaaggatgat cttgtagaga
      901 gggcaaagga actggaatgt ctttatctag tcgatgaggc attaacagag ctttctttat
      961 cggacagcct tgaaaaaatt acatccatta ttcccttagg ctttcgaatt ccccataatt
     1021 gcaccgttgt tattgaactt gacggacagg tttacaacca gaaagcctcg gtaaataata
     1081 cggatgaatt gtgttctgat attaccgtta acggaaaatc ccgtggatac attaaggtcc
     1141 tgtatccaaa acaaactttc cctgagaatg atgttgtctt tctcgaacaa gaatcccggc
     1201 ttttgaatac catagcaaaa aggatttctg aaaccatcga taaaagggat tcggttaaaa
     1261 agttcaacta cagaaaccgt tgggaagcta ttttggatct gcttcaaaaa actgatcatg
     1321 aaatcctgct ttatgtttgc gaaaagatgc tttctttatt gtccagaatc aaccctcctt
     1381 tagtccagaa tgtctttacg gaaatgaatt ggtcaaagta cgatacccat ggtgaaatta
     1441 attttccgtt ggaaaccctc cctgaagtca atataatcca cttaagcaga attctatttg
     1501 agaattccac aaaaatcctg gaggactcac agatttatga ctatatcaat ttatgggttt
     1561 atcagggaaa gacctacgaa ctgattaaaa tagtcgataa aagggattcc gatataaaga
     1621 aaatttccca agcgttatcc gcatatgtga aagcagttaa aaataacaaa atgtccagtg
     1681 aaacaacaag aagatggcta agggtggaat taacacgacg ctttttaacc gataatccga
     1741 aaaccatatc caaaatccat aactatatta atgtagaggc ttttataaag cttttagata
     1801 cctttatatg ttcaccgaga agtattggcc ggattggcgg aaagggctcc ggatttttct
     1861 tggcaaacca gataattgag acttacaaaa aggaatttcc tgagcttgag aatattattg
     1921 ctccaaaaac ctggtatatt tcttccgatg aatttaagta cttccttgaa gacaatggat
     1981 ttgaagaatt aaatgaacat aaatattcag atattaccga tgtccgtacc agttacccga
     2041 gaataatcca aatgttgaaa aatgcccgat tatctcctta tatactgaac gaacttaacc
     2101 aggttctgga tgcctgtgaa gattgtccgt taattattcg ctcttcgagc ctgttggagg
     2161 atcaaatcga ttcctcattt tcaggcaaat acaagagcct ttttataaca aacaccggta
     2221 ctagagcgga acgcatgaag caattggtag atggtatttt agaagtctat gcctcaatct
     2281 tcagtgcaga ttcaattcaa taccggaaag aacgcaattt attggactgc aacgatcaaa
     2341 tgggaatttt gattcaagag gtagtaggct gcaaggttgg acgatactac tttccactgt
     2401 ttgcgggagt ggcattcagt aacaatgaac tgcggtggtc accccgtatt aaacgggaag
     2461 atggattaat gcgtatggtc atgggattgg gtacaagagc cgttgaccgt gttgtagaag
     2521 attatccgat cttactgtct cccggtcagg caaatcttcg cgttaaccaa tcaccctatg
     2581 agcttttgaa atattcgccc caatatattg atgtaattga tttggaaaac aaccggtttc
     2641 ttactctgcc gatttccgac ttgattaagg aatgcggaga tcagattccc aatatcaacc
     2701 ttttggcatc ggtacttaaa aatgatattg tcatggacgc aaaccccttg accacggatt
     2761 ttaaaacaga caaaatcatt ataacctttg atggatttat taaaaaaact cctttggtaa
     2821 aacaggtcaa atccattctc tctttactgc agaccgaatt gggctatccc gttgatattg
     2881 agtttgccag tgacggaaag tatttttatc tgcttcaatg ccgtccgcaa agcagaaacc
     2941 atgacaacgc tccggttgct attccggcaa acattccggc tcaatgcact gtttttactg
     3001 ccagcaaata tgtttcaaac ggaaaagtca ccggcattaa gaccgttgtt tatgttgacc
     3061 cgaatgaata ctccagattg gaatcccacg aggatttgct cgatatccgt aaaataatca
     3121 gcgaactgaa ccggttattg ccacgccgaa gttttatcct gataggaccc ggccgctggg
     3181 gcagccgtgg agatattaaa ctcggagttc cagtcgctta ttccgacatt aataatacgg
     3241 ctatgttgat tgaagtggca caaaagcaat ccaaatatga accggagttg tctttcggaa
     3301 cccacttctt tcaggatctt gttgaagaga atattaaata ccttcctctc tatccggagg
     3361 ataacgatat catattcaat caagcatttt tcagaggctc aaaaaatact ttacccgata
     3421 ttctaccgat gtactcccat ctttgtgatg tagtcaaagt aataaatatt gaggaaactt
     3481 tttttgataa agaactgatt gtattaatga acggagacct tcagaaagct atagcttatc
     3541 ttgaacaacc ctctcgtgta aattccgaat caaactccga ttttacttct gcacccgtaa
     3601 ccaatgatta tgatgaagag ggatggaagt ggcggcatta catggctgaa caaattgccc
     3661 atcaggttga catggatgca ttttccgtaa aaggaatata tcttttcggc agcaccaata
     3721 actgcacagc caggttaaac agtgatattg accttttgat tcactttgac ggaaacgaag
     3781 aacaaaaaaa aagcctgaac acatggctcc agggttggag tatggcactg gcggaaatta
     3841 attaccttaa gaccggttat aaatccagcg gtttattgga tatccattat atcaccgacg
     3901 atgatattaa aaacagaacc tcctatgcca tcaagattga ttccgtcttg gatcctgcct
     3961 gtcctctgag gatacgggca aaataatata aaagtgtttc cggtttaaag gaacacccaa
     4021 aagaacgata catttttacg gaaagctaaa agagatgccc cctctctata aaagaagcat
     4081 ctctttttat attatgcctt aaacaaggct tatttgaaaa ttgcattgtc cagtctgtca
     4141 aacagttttt gagcattacc cgatccggta cagttttcca agacaatttt agcaggatta
     4201 ccgttgcctg tgaacccatg ctttgcatta ccttttgcta cgcagtttat aagtacatga
     4261 ggcactgaag cagtgtcatc ccccagttta tagccgttac cgtctccgcc ggtctttttt
     4321 ccgttagtat cgtatccatt tccaatagcc tcacagtttt caagggtaac aacaccaata
     4381 ggtccggtat cggattttgt atacatatcc cagccgtcat cacaattgta aagtgcttta
     4441 cagtttctaa atacgtttcc tcttccgcag gtaagcttcg gtgcaaaacc gtcagcatcc
     4501 tctctgcccg aatcaaaatt cagagtcgaa atacaatcta ctataaggtt gttgctcggc
     4561 cactggtcaa tcgatttata ggaagtattg taccttgaca actgtaatcc cgagtcgtga
     4621 ttttctgtaa acgtacaagc ttcgattata ttgttgcttc ctgctagaag cattccgtta
     4681 tctcctgctc cttttatggt tatacccttg atatgccagt agttggctgc aagtaccgct
     4741 cctctgttgg atgaattctc actcatggct gaaaaatcta taaccggttt atcgcttcca
     4801 taagcaacaa tcctcttcat tgcactcgat gtaccgtcat ttccctcggc tatcactatt
     4861 gtttttgagt aattatagtt tccgcccttc aaataaatag tctgccccgg ttttattgca
     4921 ttgatagctt gctgcaaatc cattgatccg ttaggttcaa gaataatatc accctcggtg
     4981 ggagtacttg tcggcggcgg tgtcgtaggc cgtgatgtat agggtgttgg tgtcggtgta
     5041 gacgcagatg ttggtgtcgg tgtcggttga ttaccgttgt taacaggcaa actgtcgatt
     5101 tttcttaata taaatctttt cagtattgtg atatcagttg agtttacttc attgtcaaga
     5161 ttcacgtctg cggcttttaa ttgatctgat gtgaaatcca aacgccttag tatatatctt
     5221 ttcaatattg ttaaatccgt tgaatccacg agcccgtctc cattgatatc accatatact
     5281 atttccggat gataagtggt tggagttggt gtaatttgcg gcggtgttgt tttaggtgac
     5341 gatgtttgcg gcggtattga cggtgtagga atactgtttc cctgaacacc ggcatattgt
     5401 aaaacagttt ctttaacctg gcttgcaggt tgtaacaccc gcgaataatc gtaaggcgga
     5461 ttgaagctgc atgttgaggt agtgggctga tttcccttac aatcaacaaa tatgttgtcc
     5521 ctaacatccc aatagccgat attactgctg taataagagc caatggggcc ctctgcaaat
     5581 cccgttttgc tatccaccga accgcatcct gcattttcaa aaacatttcc ttcaattctt
     5641 aattttgcac ctacccttga gttgatccca ctgccggaaa cattcacata gtagttgttg
     5701 aacatatgcc ctgtaccgct tctataactc ggtgtacggg atttaacgtt tgaatataca
     5761 ttgtggtgga aagtaatttt tctgtcatag tcatcactgt ccgatgagcc gataagacta
     5821 gtcttgtatg aatcatggaa ataattccat gaaactgtaa tgtattcact ggacttttta
     5881 atatccaaaa gtccgtcata ccagtcaacg tctcctcctt cggtatctcc gtcattggga
     5941 tttactattc cgtcaccgtt acagtcacca atcatattat acaattcaca atggtccacc
     6001 caaatgtttt gtgaattttc aacgccgatg gcatccatag gagctctggt gtggtgtatt
     6061 tttaaattct gaacaatgat atttttggat ttaacaatat tgattccaca gccttccaac
     6121 tcccctttat cccctactcc caaaaatgta atattgctca cctctttaca agcaataact
     6181 tctgaaccgg ttagctttct gtcaaacttg attatcaacg gtgaagtgtc cttgttcttt
     6241 tttctcgcac ttaatatatc attgacctgt gatacgctcg ttgccgttat ttctgtccct
     6301 ccagtacctc ccgtggttcc gccgtttagt gttgcaaatc caatcagttc aaagcttagc
     6361 actgcttgaa catttaatgt ggttgcagtt gctgcaatca cagatataag gattgtacac
     6421 acgactagca acgacatctt tgctgaaaac ttctttaaca tacattaccc tcccttaaaa
     6481 atttgaattt gatcttttta cttatttaat ttatacgagg aataaaaaac atatgggtca
     6541 taaattgaca aacgaaaatg atatcgaaaa tagcgtctta ttctttatag aagaaattct
     6601 tatttggtgg aattggtcca ttattgaatt attgtgaatg cctgttcctc tctacagctt
     6661 aattataaaa aaaccgccgt atttcggtca atgaacactt tctatttgcg aatatacatt
     6721 ttttaatatg ctcctatcat ttcggatttt tccgggctct ttactttcgg aattgaactt
     6781 tcaagaacac tttttacatt ttacgtttca ataattttcc ttgcaaaata taacagcttg
     6841 tatccctatg gatttttctg ctataatggt attataattg gtatatttag ctcagtattc
     6901 agactctaat ataccgattt tgatatactg gttttaatac gctgttttta atatgcagtt
     6961 tttaaatatg cagtttttaa atatgcagtt ttttagtata ttgttttatt cttgatttta
     7021 tatacattct attataaata tatttatatt cttttatatg tttgaggggt agggtaatat
     7081 tgaaaaagaa atatattttt ttactgattt ctattgaaat aatcttgatt gctttgttga
     7141 tttatttctt cgttccgaag tccggtctca atactcatgt tgaagcggtc aacatgaacc
     7201 ttactaatta taaatatgac tattatgtcc aacgaaattt aattaaaatt gcaggtgatg
     7261 taggcactgc atcctatgtt ttcaaaggta
//
LOCUS       DAOOJC010000065         7253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_8620_length_7253_cov_4.419978, whole genome shotgun sequence.
ACCESSION   DAOOJC010000065 DAOOJC010000000
VERSION     DAOOJC010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8620_length_7253_cov_4.419978"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2155)
                     /gene="glgB"
                     /locus_tag="PLA01_02965"
     CDS             complement(<1..2155)
                     /gene="glgB"
                     /locus_tag="PLA01_02965"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HPD00293.1"
                     /translation="MNTTAKIDEVLRVINAEHPDPFSVLGMHRLESQDAMVVRAYLPN
                     AQSIDVVPLSGGKRYKMDRIHENGFFEVVMDDRNDFFKYVLEITDDMGNSFSIHDPYS
                     FMPVLSEFDLHLFGEGNNHKIYEKLGAHKMTIDGIEGTFFAVWAPCAKRVSVVGHFNQ
                     WDGRRHPMRTRGPSGVWELFIPGINQGEIYKYEIKTPHDEIYVKADPYAFYSELRPNT
                     ASIVYDIDGYEWGDRDWMYERDNSNNFEKPVSIYEVHLGSWKRVSNEDENGFHTYREL
                     ADMLVEYVKDMGYTHIELLPISEHPFDGSWGYQITGYYAATSRYGEPKDFMYLVDKCH
                     QNGIGVLIDWVPAHFPKDGHGLARFDGTALYEHYDPKQGEHPDWGTHIFNYGRNEVKN
                     FLIGNALFWLDKYHIDGLRVDAVASMLYLDYGKKDGEWIPNRWGGKENVDAIDFMRQL
                     NSTVYKYHPGVMMIAEESTSWALVTKPPYAGGLGFSHKWNMGWMNDFLRYMSMDSVYR
                     KFHQNLITFSMMYAFSENFILVLSHDEVVHGKCSMISKMPGDYWQKFAGLRASYGYLY
                     GHPGKKLLFMGGEFGQFIEWNYKQSLDWFLLDYEMHKKMQDYVRDLNALYKREKALYE
                     VDFNYEGFEWIDCNDTEHSVISFIRKGKDWHDSLIFVCNFTPVPHDEYRIGIPFDTVY
                     DEIFNSDWDKYGGSNVGNFGEIHTEKEPMHNKPYSM"
     gene            2660..3838
                     /gene="nagA"
                     /locus_tag="PLA01_02970"
     CDS             2660..3838
                     /gene="nagA"
                     /locus_tag="PLA01_02970"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HPD00294.1"
                     /translation="MIKNGLVLESGKGFNKKDVLIQNGKIERIDVNIAKPEEEAFKVL
                     DAQGCWVVPGLVDIHTHGAKGIDFMDAGRSELEELSLFYASKGVTSFLAATMTDSAER
                     IKDALKNINHAINEGLPGARIAGINMEGPFINPGYRGAHPQEHIIKPDLRLIEEFIEI
                     SGNNIRLMTIAPELEGVKEITRELLKEGITVAAGHSGADFICAQSAFNNGFGHVTHLF
                     NAMTGIHHRNPGLAGAALDNENVTVEIIPDAIHVHPSVVRSAIKCKTCDRTVLITDSI
                     AAAGLEPDDLKLEFAGKRITIKDGAAAFDNGVLAGSTITMIDGVKNMVQRMGIDLLDA
                     IKMASENPAKVIGIYDRTGSISEGKDADVAILDKDLNVRTTITGGIITYGRPEETGRL
                     "
     gene            complement(3869..5104)
                     /locus_tag="PLA01_02975"
     CDS             complement(3869..5104)
                     /locus_tag="PLA01_02975"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPD00295.1"
                     /translation="MLRRSFNNKKLGSFSLLLLSLVLCAGLTFLTYRLFSWYSKNALR
                     EVPIVLFASLFAGILYIAIFMVSIKLIKIVSEQHLNARELKNFKNFTDVIHRAETEIE
                     VYESLYTFVNNMHLVSHVTLFYRKDISPKEITWLRLTNERMPLCSMEPRNCPLFRFGR
                     ECSVQNIATDITCAFQLPEHKYGSYICLPITEGPVTLGILQLYSKSKYFFDETTISKL
                     KSYIEVARPIISSRRAMHQLNKEASTDQLTKLYNRSFLDSYLQNQIEVASLSKQPLSL
                     IMLDIDDFKKVNDTYGHPAGDAVLVVFSQVILRCVRRTDMVTRYGGEEFIAVLPATNT
                     DKAVSIAERIRETIAMETMPKINDIQLPNITCSLGVTTYPVFADDKEKLIKTADVALY
                     KAKAAGKNRVILYSNGMGL"
     gene            6000..7112
                     /locus_tag="PLA01_02980"
     CDS             6000..7112
                     /locus_tag="PLA01_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPD00296.1"
                     /translation="MGVKIYLSSPHMSDEGYERKYIDEAFETNWIAPLGPNVDGFERE
                     LAAKVGSKHAAALVSGTSAIHMALKAVGVGKDDIVFCQSLTFSATVNPVIYQNATPVF
                     IDSDNKTWNMDPDLLEEAFKKYPNVKAVLVVHLYGISADLDSIVEICQRHNVPLIEDA
                     AESLGTTYKGKYTGTFGDYGIFSFNGNKIITTSGGGMLVSDNEERIAKVRFWATQARD
                     KARHYQHSELGYNYRMSNIVAGIGRGQLKVLDKRIAKKKYIFEFYKRELGELEDVKFM
                     PENEWDKPNYWLTCITLSGKVKPIDIIEALEKDNIESRPIWKPMHMQPFYEGYDFIGG
                     DVARSIFENGVCLPSDTKMTDDDLNRVCGIIKGLWK"
BASE COUNT         2166 a         1436 c         1555 g         2096 t
ORIGIN      
        1 tcatagaata aggcttgtta tgcatgggtt ccttttcggt atggatttca ccaaagtttc
       61 cgacattgct tccgccgtat ttatcccagt cgctgttgaa tatctcatca tatacggtgt
      121 caaagggtat acctatgcgg tattcgtcat gaggaaccgg cgtgaagttg cacacaaata
      181 tgagggaatc gtgccagtcc ttgccctttc ttataaagga tattacactg tgttccgtat
      241 cattgcagtc gatccattca aagccttcgt agttgaaatc cacttcatac aaagcttttt
      301 ccctcttata aagggcgttc aggtccctta cgtagtcctg cattttctta tgcatttcgt
      361 aatccagcaa gaaccagtca aggctctgct tgtagttcca ctcaataaac tgtccaaact
      421 cgccgcccat aaataaaagc ttctttcccg ggtgtccata cagataaccg tagcttgccc
      481 tgagtcctgc aaacttctgc caatagtccc ccggcatttt actgatcatg gagcatttac
      541 cgtgaacaac ctcgtcatgg gacagcacca gaataaagtt ttccgaaaaa gcatacatca
      601 tggaaaaggt tataaggttt tgatgaaact ttctgtatac cgaatccatg ctcatataac
      661 gcaggaagtc gttcatccag cccatgttcc atttatgtga aaatcccagg cctccggcgt
      721 agggaggttt ggttacaagg gcccaggaag tggactcctc ggctatcatc attactccgg
      781 gatgatattt gtaaaccgtc gagttgagct gccgcataaa atctattgca tcaacgtttt
      841 ccttcccgcc ccaccggttg ggaatccatt ccccgtcctt ctttccgtag tccagataaa
      901 gcatggaggc cacggcatca acccgaagac cgtcaatatg gtatttatcc agccagaaca
      961 gggcattgcc tatcaagaag ttttttactt cgtttcttcc atagttgaat atgtgagtgc
     1021 cccaatcggg atgctctccc tgcttgggat cataatgctc atacagggct gtcccgtcaa
     1081 atctggccaa accgtggccg tccttcggga aatgtgcggg aacccaatct atcaatactc
     1141 ctatgccgtt ctgatggcat ttgtcaacaa ggtacataaa gtcctttggt tctccgtatc
     1201 tgctggttgc ggcataatag ccggttatct gatatcccca cgaaccgtca aagggatgct
     1261 ccgaaatcgg cagcagctca atatgtgtat atcccatatc cttaacatac tccaccagca
     1321 tgtccgccag ctccctgtat gtatgaaaac cgttttcatc ctcgtttgaa accctcttcc
     1381 acgaaccgag atgaacttca taaatggata cgggcttctc aaagttattg ctgttgtctc
     1441 tttcatacat ccagtccctg tcgccccact catacccgtc aatatcgtac acaatagaag
     1501 cggtattggg ccggagctcg gaatagaaag cgtaggggtc tgcttttaca tatatttcgt
     1561 catggggggt ttttatctca tatttatata tttcaccctg gtttataccc gggataaaca
     1621 gctcccatac cccggagggg cctctggttc tcatgggatg cctgcgtccg tcccactggt
     1681 taaaatggcc tacaacgctt acgcgttttg cacagggtgc ccaaacagca aagaatgtgc
     1741 cttcaatccc gtcaatggtc attttgtgag cacctagttt ttcatatatc ttgtggttat
     1801 tgccctcgcc aaacagatga agatcgaatt ccgacagtac aggcataaag ctgtaaggat
     1861 cgtgaatgct gaagctgttg cccatatcat cggtaatttc cagaacatac ttgaaaaagt
     1921 cgttcctgtc atccattaca acttcaaaaa acccgttctc atgaatcctg tccattttat
     1981 acctcttgcc gccggataac ggcacaacat cgatggattg agcattgggt aaatatgccc
     2041 ttacaaccat tgcatcttga gactccaggc ggtgcatgcc cagtacactg aacggatccg
     2101 gatgttctgc attgataacc cttaaaacct cgtcaatttt agctgtagtg ttcatcattc
     2161 tcatacctct tctttttata tttattttag tataagcata aaaccgattc ttaattaaaa
     2221 aaagtacaaa acacagcaca aaaccttaat ttgaattttt taccatatgc aattggaata
     2281 tgaatcttta aaacaaggca tttgaaacag attaattatg gtttggaaaa aatcacaaat
     2341 ttcacagtac tataatacca taaaaggtgt aaatattaca gccttttttt aaacaaattt
     2401 ttagttttgt aaaaatagcc gcatattcca agttgagcat tattgtaccg ggttgtctca
     2461 cacaagtgta ttatatgcaa aatgccattg agatatactt gctaataatg gtacacttaa
     2521 tattgtcgga gggtgcaatg tttcttctgc actttcttta ctttaataat ttagccatat
     2581 tgaatgcata ttcatagtgc aatatactgt ctgcggtgcc tgagaaattt attaaaaaaa
     2641 ggggcgggaa aagttgttct tgattaaaaa cggcctggtt ttggaaagcg ggaagggctt
     2701 taacaaaaag gatgtactga ttcaaaacgg gaaaatagag agaattgatg taaatatagc
     2761 aaagccggag gaagaagctt ttaaggtttt ggatgcacag ggctgttggg tcgtaccggg
     2821 gcttgtggat attcacaccc acggtgcaaa aggcattgat tttatggatg caggccgaag
     2881 tgagcttgag gaattatcgt tattttatgc ctcaaagggg gttacctcat ttttagcagc
     2941 cacaatgacc gattccgctg aaaggattaa agatgcctta aaaaacatca atcatgccat
     3001 taatgaagga ctgccggggg caagaatagc cggtatcaat atggagggac cgtttataaa
     3061 ccccggttac agaggggctc atccccagga acatattatt aagccggatt taaggctgat
     3121 tgaggaattt atcgaaatct ccggaaataa catccggctt atgaccattg caccggaact
     3181 ggaaggtgtg aaggaaataa cccgggagct tttaaaagag ggcattaccg tcgctgccgg
     3241 ccattccggt gccgacttca tttgtgcaca gtcagccttt aataacggct ttgggcatgt
     3301 tacccatctt tttaatgcca tgaccggtat tcatcacaga aacccaggcc ttgccggagc
     3361 tgctctggat aatgaaaatg tgactgtgga aatcatcccc gacgccattc atgtgcatcc
     3421 atcggtggtc agatcggcaa taaaatgtaa aacttgtgac aggactgtcc tgataacgga
     3481 ttcaattgct gcggcaggac ttgaacctga tgatttaaag cttgagttcg caggcaaacg
     3541 cataacaata aaggacggag cggcggcctt tgacaacgga gtgctggcag gcagtaccat
     3601 cacgatgatt gacggtgtga aaaacatggt tcaaaggatg ggcatcgatc ttttagatgc
     3661 aataaaaatg gcctcggaga atccggcgaa ggtaataggt atttatgaca ggacgggcag
     3721 catttccgag ggtaaagacg cagatgtggc aatacttgat aaagacctga acgttcggac
     3781 aacaataacc gggggaataa ttacttatgg gaggccggag gaaacaggac gcctatgacc
     3841 ccctatagaa ttggaaaacg gcatggacct aaagtcccat gccgttggaa tacagaataa
     3901 cacggttttt accggcggcc ttggccttgt acagtgcgac atccgccgtt ttaataagtt
     3961 tttctttatc atctgcaaaa accgggtatg tggttacacc aaggctgcag gtgatattgg
     4021 gaagctggat atcatttatt ttcggcatgg tttccattgc gatggtctcc cgtatacgtt
     4081 cggctatgga aactgcctta tccgtattgg ttgcgggaag aaccgctata aattcctctc
     4141 ctccgtaacg ggtaaccata tcggtacgtc tcacacacct taagataacc tgtgagaaca
     4201 ccaccagcac ggcatcaccg gcagggtgac cataggtatc attgaccttc ttgaaatcat
     4261 cgatatcaag cataatgagg ctcaagggct gcttggaaag actggctacc tcgatttggt
     4321 tttgaagata agaatccaaa aagcttctgt tataaagctt ggtgagctgg tctgtagaag
     4381 cttccttgtt cagttgatgc attgcccttc tgctgcttat aatcggcctt gccacctcta
     4441 tgtaggattt gagctttgat atagtggttt catcaaaaaa gtattttgac tttgaataaa
     4501 gctgtaaaat acccaatgtg acaggacctt cggtaatagg cagacaaata tagctgccgt
     4561 atttatgctc cggtagctga aatgcacagg ttatgtcggt agcaatattt tgtaccgaac
     4621 attcgcggcc aaatctaaac aaaggacaat ttctaggttc catggaacaa aggggcatcc
     4681 tctcgttcgt caacctaagc catgtaattt cctttggaga tatgtctttg cggtagaaca
     4741 gcgtcacgtg gctcaccaaa tgcatgttat taacaaaagt gtagagactt tcataaacct
     4801 cgatttcagt ttcagcacga tgtataacat ctgtaaagtt cttgaagttc ttgagctccc
     4861 gtgcatttaa atgctgttcc gatacaattt taatcaattt aatcgaaacc atgaaaatag
     4921 caatatataa aatacccgca aacagtgaag caaataaaac tatcgggact tcacgaaggg
     4981 catttttgct ataccatgaa aaaagtctat aagttaaaaa ggttaatcct gcacaaagaa
     5041 ccagagaaag caacaataag ctaaaagaac cgagtttttt attgttaaaa gatcttctaa
     5101 gcacgtacat cacccttcct aaaggatgta taacaacaca aataagttca acgtttcgta
     5161 gtatgtctta gcggctattg gcaattgagt gttaaatatt gacgaagtgt cgaataaaac
     5221 ggtttattaa ggtaaaaatt gttgcaaatg tctgtccggc attaattata ttaactttct
     5281 attccttaaa aagtcattaa ttttttactt gcatatatta atataatacc ataaatgcgg
     5341 cattttgtga acagttttta gcataatcaa aatatttctc acacggcaga gcaacgattc
     5401 tttccaccat aatacaaaaa acctcataag gcttgtataa tgcacttttg actgttatgc
     5461 aattatacat aattaagtaa caatcttaag agattgtttc aattataata aatctttcga
     5521 tataagtaaa tgaaatttaa ataatttaat aataatttaa taattcgtgt atagatttat
     5581 attattctaa gactccaact tctataaggt ggatttcctc ttataatctt caggtggagt
     5641 agagtctcca cctgaagccc cgatgtttag ctttagctaa acgagttcac ttttgtgact
     5701 tgacaggaga tatgcaatag ggtaattttt agtagtaaag taaaatgcaa tggtataaac
     5761 agagtcattc agatatatat ttaaatgata gaaaatgacg gtcataatta attgacaggt
     5821 tagagttcgt agagttataa taatttatga ttattcattg gattttgaaa agatataaat
     5881 aattagcaat aaaaaaaaca gtgggaaaat ggtagttttt cgcaattttg ctgtttgagt
     5941 tcaaaataga tagttatcat gttaaggata taattttgtg aggaaggaag tcatggaaaa
     6001 tgggggttaa aatttacctt tcatcacccc atatgagcga tgagggatac gaaagaaagt
     6061 atatagatga agcttttgaa accaattgga ttgcaccttt gggaccaaac gtggacggat
     6121 ttgaaagaga gcttgccgca aaagtcggtt caaagcatgc tgctgctttg gtttcgggca
     6181 cttcagccat tcatatggct ttaaaggctg taggggttgg aaaggacgat attgtatttt
     6241 gtcaaagcct taccttttct gctaccgtta accccgttat ttatcagaac gcaactccgg
     6301 tttttataga cagtgataac aaaacgtgga atatggatcc cgatcttttg gaagaggcct
     6361 ttaagaaata cccgaatgta aaggctgttc tggttgttca tttatatgga atttctgccg
     6421 atttggatag tatagttgaa atttgccaga ggcataatgt acctttgatt gaggatgctg
     6481 ccgaaagctt gggtactact tataaaggaa agtacaccgg tacttttggt gattatggca
     6541 tcttttcctt taacggaaac aaaattatca ccacctccgg cgggggtatg ctggtttccg
     6601 acaatgagga gagaatagca aaggtaaggt tctgggccac ccaggcaagg gataaggcaa
     6661 gacactacca gcacagcgaa ttgggctaca actacagaat gagcaatatt gtagccggta
     6721 tcggacgggg acagctgaag gtactggaca agagaattgc gaaaaagaaa tacatctttg
     6781 agttttacaa gagggagctt ggggaacttg aggatgttaa gtttatgccc gaaaacgagt
     6841 gggataaacc gaattattgg ctaacttgta taactttaag cggaaaggtt aaacccattg
     6901 atattataga agctcttgag aaagataaca tagagtctag gcctatatgg aaaccaatgc
     6961 acatgcagcc gttttatgaa ggatatgatt tcattggcgg cgatgtagca agaagcatat
     7021 ttgaaaacgg tgtttgtctg ccgagtgata cgaagatgac cgatgatgat ttaaatagag
     7081 tatgtggaat tattaagggg ttgtggaagt agaatgcaaa ctgaggttaa gcaaaatata
     7141 gaatcggcct tgcagccgct gaaaaaaggc ggcttatata gaaggtattt taaaagacct
     7201 atggattttg tattgtcttt gactgcaatt attgtgctta gtcctgtgtc ttt
//
LOCUS       DAOOJC010000066         7240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_8642_length_7240_cov_3.854001, whole genome shotgun sequence.
ACCESSION   DAOOJC010000066 DAOOJC010000000
VERSION     DAOOJC010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7240
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8642_length_7240_cov_3.854001"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PLA01_02985"
     CDS             complement(<1..837)
                     /locus_tag="PLA01_02985"
                     /inference="COORDINATES: protein motif:HMM:NF025298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HPD00297.1"
                     /translation="MSYKVTISDGTVLEKCINDIRFYTLQSDDYSSRHTNNRSSIKIT
                     GYIDTDESTVGLYNWALLPATNPDCYKEITVEHYKSGKLLRKVTFSKAFVVDYSEEYS
                     NDEGVGFFMLHIRQIYAKNVEVDAPDDAPDETADANKTDSDSVPETEEQTQSQEAIEE
                     KSSAGKAVMSFTERLEKKKEIKDNITIVEYGEHFTKDGRKKALKPNIQYTTPEGYTYR
                     TDELGRIIYCEGTLQKGVAERNKYVQRTVGGKDRLPDDEGGHLVASIFKGSGDFDNLV
                     PMD"
     gene            complement(1126..1770)
                     /locus_tag="PLA01_02990"
     CDS             complement(1126..1770)
                     /locus_tag="PLA01_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPD00298.1"
                     /translation="MSSDKKISIAVIKRLPRYYRYLSDLLNLGITRISSKELSSRMGI
                     TASQIRQDLNCFGGFGQQGYGYNVEYLYQEIGKILGVHERFNVIIIGAGNMGQALANY
                     TNFEKRGFKLIGIFDVNPDLTGKKIRDIEIMSMDDIERFISENKVDIAMLCVPYEHTP
                     TVADKVARLGVKGLWNFSPMDLKVPHDVIIENVHLSDSLMVLGYKLAEKYKREK"
     gene            2020..3933
                     /gene="abc-f"
                     /locus_tag="PLA01_02995"
     CDS             2020..3933
                     /gene="abc-f"
                     /locus_tag="PLA01_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HPD00299.1"
                     /translation="MIVLSCNNIGLSFGASVILKGISFSINNTDRVGVVGVNGAGKST
                     LFKIITGSYKPDTGEVYTAKNSKIGYLEQSSGLDSTRSIIDEILSVYSHFIEMEKRIK
                     DLEKSISSEKDQNALNGLMKEYARLTEEYSRLGGYEYNSRAIGVLKGLGFSEDQFALN
                     VKSLSGGQKTRLALARLLLTEPDILLLDEPTNHLDIGAIEWLEDFLSSYKKCVMVISH
                     DRFFLDRITNMTLEIENCECQLYNGNYSEYLKKKAVNREIQQKHYELQQKEIAKMEEF
                     IEQQKRWNREKNLIAARSRQKAIERMDKIEKPKNLPQKIKIQLKSNVSSGNDVLFVEG
                     LGKEYPGKPLFKNVSFNIRKNERVFLLGPNGCGKSTLLKILTGRIDKYSGQYEYGHNV
                     NLGYYDQEQEDLDPENTVIDEVWDANERLTQTEIRNTLAMFLFKGEDVLKTVGNLSGG
                     EKSRISLIKLMLSESNFLLLDEPTNHLDINSREVLENSLSDFDGTLLIVSHDRYFINK
                     LATRIIELGGNSCIDYNGNYDDFQQYKIKICALSGNGTKENNVSSAKLEHMATKEEKA
                     RKRKLEKQIMDTEKEINSTEARIKEIEDQMTLDEVVSDHVKLMELNDEHNRLNERLEE
                     LYVLWDDLQSQSD"
     gene            4230..5792
                     /locus_tag="PLA01_03000"
     CDS             4230..5792
                     /locus_tag="PLA01_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPD00300.1"
                     /translation="MNILTIKILLYKGMMSFMWYIVQPGDSLYTISQRFGVTISQIRE
                     ANQITGNIIYIGQRLYIPLESQTPIIYTVKAGDSLYLIAQRFDTTVDSIMALNNLTST
                     QLRVGQQLTIPIYTEVVVNVDNANIRSGPGTNYSVLVRMARGARLPVTGFSNSWYRIR
                     LFNGRDGWISGSIATRRVYDGSKPITGVLGFYTLEEGPTLPSSFASFSNNTGLLSSTG
                     LFMFRISAANPTTIEKFGDFTDQDVRNLVAIAHRNNVKIMAVVHNLLYRPGGTTLAKN
                     VVKVLVSDPRNRNAFALNLVNLIEKYGFDGVNIDIEDVFIEDSDNLSLLYADIAEVLR
                     PRGYFFSASIPSRVSDEPFNSFSDPFNYSAIGRAVDEFVVMLYNEFGWPGSPPGPPVT
                     IGWMNRVLQYTMTKMPKNKIVAAVSVFGFDFNLTTGRNTYVTYQGAINLAQRYNSRII
                     FNEERQTPMFTYTDAQGNRHEVWFENARSLRAKIQLAWDLGIKGVALWRLGMEDPAIW
                     TMLRDEVVVKKF"
     gene            complement(5840..6556)
                     /gene="cwlD"
                     /locus_tag="PLA01_03005"
     CDS             complement(5840..6556)
                     /gene="cwlD"
                     /locus_tag="PLA01_03005"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HPD00301.1"
                     /translation="MILVLRKDKIALIALVFVLSILLISINIGSNFNAVEVTNANVKG
                     TIVIDAGHGGEDPGAVSDYSGLKEKEVNLFIAKRVKELLEAENYRVIMTREEDILNYR
                     EGTRGYDNKRAQDLMNRKKRIDESGADIAVSIHLNKFSQTQYYGAQVFFPPGSQESKE
                     LAEIIQKALREKVDPSNKREALVKDSKLIILRNIKVPTVIVECGFLSNRQEEQKLSDK
                     DYQEKLAQAIKEGIVQYFEK"
     gene            complement(6728..7162)
                     /locus_tag="PLA01_03010"
     CDS             complement(6728..7162)
                     /locus_tag="PLA01_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00302.1"
                     /translation="MKRKLIIALLFALLITGIPAVALAKPSNAKGTETVFTEPVIFET
                     TITVDKKGGKFDVGFVTIQFNNDWLPGGQYPAEFDVKICARDGEPCIEITPDVEAFSK
                     PVLIKTDKYDGYLYDETTGKNIFVSIKSQVVVAKHFSWYRFR"
BASE COUNT         2197 a         1511 c         1350 g         2182 t
ORIGIN      
        1 atccattggc acaagattgt caaaatcgcc agaaccctta aaaatacttg ctactaaatg
       61 tcctccttca tcatcgggca atcggtcttt gcctcccact gtcctttgta cgtatttatt
      121 tctttccgct actccttttt gcaaagtacc ttcacaatat attatacgtc ctaattcgtc
      181 agttctgtat gtatatcctt ccggtgtagt atactgtata ttaggcttta aagccttctt
      241 cctcccatct ttggtaaaat gttcgccata ttctacaata gttatattat cttttatttc
      301 ttttttcttt tcaagtcttt cagtaaaact cataacagct tttccggcac ttgatttttc
      361 ctcaattgct tcctgactct gtgtttgttc ctctgtttca ggtacagaat ctgaatctgt
      421 tttatttgca tcggctgttt cgtcaggtgc gtcatcaggt gcatcaactt caacattttt
      481 tgcatatatt tgtcttatat gaagcataaa aaaacctaca ccttcgtcat tggaatattc
      541 ttctgaatag tctaccacaa atgctttgga aaaagtaact ttccttaaca acttacctga
      601 tttatagtgt tctacagtaa tttctttata acaatccgga ttagttgccg gaagcagtgc
      661 ccaattataa agtcctacag tgctttcgtc tgtatcaata taacctgtaa ttttaatgga
      721 acttctatta ttagtatgtc tgctactata atcatcagac tgtaaagtat aaaatcggat
      781 atcattaata catttttcca atacagtacc gtccgaaatt gtaactttat aagacatttt
      841 taccctcctc ttcgattctt tgcaaattat tataacataa tatatagttt aagttaaacg
      901 aaaataaaaa aattaggaga ttaaaagttg tagtaaaagt aataaaaagc ttagacacta
      961 ctactttaaa agaaaaaacc aaagatgcaa aacgctcaaa aaagctcgga ggtttcaatg
     1021 ccttccgagc ttttgttagt gggatttcac gaagtgaaat gaccaaaaag cgatgggggc
     1081 tcgatgcccc cggagctttt atttatacac tatatgattt ctaaactact tttcgcgttt
     1141 atatttttct gccaatttat atcctaatac catcaggctg tcactgaggt gaacattctc
     1201 aattattaca tcatgaggaa cttttaaatc catcggcgaa aagttccata atccttttac
     1261 cccaaggcga gctaccttgt cagcaaccgt aggagtatgc tcataaggta cacagagcat
     1321 tgctatatcc actttatttt cacttataaa ccgctcgatg tcatccatgg acatgatctc
     1381 aatatccctt atttttttcc ctgttaaatc ggggttaaca tcaaaaatgc cgatgagctt
     1441 gaagcccctc ttttcaaagt tggtgtagtt tgccagggcc tgccccatat ttcctgcacc
     1501 gattataata acattaaacc tttcgtggac acctagaatt ttacctattt cttgatatag
     1561 atactcaaca ttatagccat aaccttgctg tccaaaccct ccaaagcaat tcaaatcctg
     1621 acgtatttga gaagcggtta ttcccatcct ggagctaagt tcctttgacg atatccgcgt
     1681 tatacccaga ttaagcaaat cggaaaggta tctgtaatac ctgggcagac gcttgatcac
     1741 tgcaattgat atttttttgt ccgaactcat cattttcacc ccaaatcgtt tttgatggta
     1801 atttacattc ctcccttaaa taaaaaacca atgtgattat atcatctttg ttattttatt
     1861 gtcaatattg ctaataagcc gtatttttgc tacaattgat taggctggtg ttccgaaaca
     1921 aaattcgatt tgtgaaagaa caccaaccgg gtagttttac gcatttgcat catgtacccg
     1981 cacaaacaag ggaagtggct ttgagggtgg tttattgtta tgatagtttt gagttgcaac
     2041 aatataggtc tgtcctttgg tgccagtgta atcttaaagg gcatttcttt tagcataaac
     2101 aataccgaca gggttggagt tgtaggagtt aacggtgcag gcaaatctac gctttttaaa
     2161 ataataaccg gctcatacaa accggacact ggagaagttt atactgccaa aaactcaaaa
     2221 attgggtatc ttgagcaaag ctccggtctt gattctaccc ggagcattat cgatgagatt
     2281 ctttcggtgt actcccactt tatcgaaatg gaaaaacgaa ttaaagatct cgagaaatcc
     2341 ataagcagcg agaaagatca gaacgcatta aatggattga tgaaggaata tgcccgttta
     2401 acagaagaat attcccgttt gggcggctac gaatacaaca gccgtgcaat aggtgtgctg
     2461 aaaggtttgg ggtttagtga agaccagttt gctttgaatg taaaaagctt aagcggcggt
     2521 caaaaaaccc ggcttgccct ggcaaggctg ctgcttacag agcccgacat tctccttttg
     2581 gatgagccca ccaaccactt ggacattgga gccatagagt ggcttgagga ttttctttca
     2641 agctataaaa agtgtgtaat ggttatatcc catgacaggt tttttcttga ccggatcaca
     2701 aacatgactc tggaaataga aaattgtgaa tgccagctct ataacggcaa ttactcggaa
     2761 tacttgaaga aaaaagctgt aaacagagaa atccaacaaa agcactacga attgcagcaa
     2821 aaagaaatcg caaaaatgga agaattcata gagcagcaaa aaagatggaa ccgggaaaag
     2881 aaccttattg ctgcaagaag cagacaaaaa gcgattgagc gtatggataa aatagaaaaa
     2941 cccaaaaatc tgcctcaaaa aataaagata cagttaaaaa gcaatgtgag cagcggaaat
     3001 gacgtacttt ttgttgaggg ccttgggaaa gaatatcccg gaaaaccatt attcaaaaat
     3061 gtcagtttta atataagaaa aaatgaaaga gtgttccttt taggtccgaa tggctgcggg
     3121 aaatccactc ttcttaagat tctgacaggc agaatcgata aatacagcgg acaatatgag
     3181 tacggtcaca acgtaaattt aggctattat gaccaggagc aggaggatct tgatcctgaa
     3241 aacacagtaa tcgatgaagt ctgggatgcc aatgaaaggc ttacacaaac cgaaatcagg
     3301 aataccctgg ccatgttttt gtttaaaggt gaggatgtac taaaaaccgt gggaaatcta
     3361 agcggtggcg aaaaaagcag gatatccctg attaagctca tgctatcaga atctaacttt
     3421 cttctgctgg atgagcctac aaaccatctg gacataaact caagagaagt gctggaaaat
     3481 tctctttcag actttgacgg aaccctatta atcgtatccc acgacaggta tttcattaat
     3541 aagcttgcta cccggattat tgagcttggc ggtaattctt gcattgatta caatggcaat
     3601 tatgacgact tccagcagta taaaatcaag atttgtgccc tttccggcaa cggaacaaaa
     3661 gagaacaatg tatcttccgc aaagctggaa catatggcaa ccaaggaaga aaaggcacga
     3721 aaaaggaagc tggaaaagca aataatggat acagaaaagg agattaactc cactgaagcc
     3781 cggattaagg aaattgaaga tcaaatgacc ctggatgaag tggtcagtga ccatgtaaag
     3841 ctgatggaac ttaatgacga gcataacagg ctcaacgaaa gacttgaaga gctgtatgtt
     3901 ctctgggatg atttgcagtc tcagagtgat taaggattca tcgagcaaaa agcgaagaaa
     3961 agtgttccag aaacttaagt tacgcaagtc tagagactgt aaatactcct cggggtcgag
     4021 caaaactcgc ttcgcgaaaa tggatgctct ccctttcctc gtcgtattaa cagtctctac
     4081 aactagcttc acaatgtttc tttcacattt ttcttcacat taatatcttc gccataatat
     4141 cttcacttta tttcttcgct tttatatctt cacttcaatt atattcaccc gaagcaaaaa
     4201 acaagattct tgaaccttca accggctgta tgaatatact gacaataaag attttacttt
     4261 ataaaggaat gatgagtttt atgtggtata tcgttcagcc aggagattcc ctttatacaa
     4321 tttcacaaag gtttggtgta acaatatccc agataagaga ggcaaatcaa atcacaggca
     4381 acattatcta tatcggccag cgtttatata ttcctcttga aagtcagaca cccataatat
     4441 acacagtaaa agccggagac tcattatatc ttattgccca aagattcgac accaccgtag
     4501 acagtatcat ggcattaaac aaccttacca gtacacagct tcgggtagga cagcagttaa
     4561 ccataccaat ttatactgaa gtagtggtta atgtggacaa tgcaaacatt cgaagcggtc
     4621 ccggaacaaa ttacagtgta ctggtaagaa tggcaagggg tgcaaggctc cccgttaccg
     4681 gttttagtaa tagttggtac aggattcgtc tttttaacgg aagagatgga tggatttccg
     4741 gtagtattgc aacccgcaga gtatatgacg gaagcaagcc aataacagga gttttgggtt
     4801 tctataccct tgaagagggt ccaacacttc ccagttcctt tgcatccttt tccaacaata
     4861 cgggtttgct ttcatcgaca ggactgttca tgttcagaat aagtgctgcc aaccctacca
     4921 ccattgaaaa attcggtgat tttaccgatc aggatgtccg caaccttgta gcaatagccc
     4981 atagaaataa tgtcaagatc atggcggtcg ttcataacct tttatacaga ccgggaggaa
     5041 caacccttgc caaaaatgtt gtcaaagttc ttgtatcaga tccgcggaac aggaacgcct
     5101 ttgccttaaa ccttgttaac cttatagaga aatatggttt tgacggcgtt aacattgata
     5161 ttgaagatgt atttattgaa gacagtgaca atctctccct attgtatgcc gatattgcag
     5221 aagttttacg acctagagga tactttttct cagcctccat accttcaagg gttagtgatg
     5281 agcccttcaa ttcgttttcc gatccgttca attacagtgc aataggtagg gctgtggatg
     5341 agtttgtcgt aatgctttat aatgaattcg gctggccggg aagtcctccg ggaccacccg
     5401 ttacgatagg ttggatgaac cgggtgcttc aatataccat gacaaaaatg ccgaaaaata
     5461 aaatcgttgc agcggtttct gtctttggat tcgattttaa cctcactaca gggagaaata
     5521 cttacgtaac gtatcaaggg gcgattaacc ttgcccaaag gtacaacagc agaataatct
     5581 tcaatgaaga aaggcagaca ccgatgttta cttacaccga tgctcaagga aaccggcacg
     5641 aggtatggtt tgagaatgcc cgcagcctga gggctaaaat ccagcttgcc tgggaccttg
     5701 gaattaaagg agtcgcctta tggaggcttg gaatggaaga tcccgcaatc tggaccatgc
     5761 ttagagatga agtagtcgta aaaaagttct aaaaaaagag agtcaggaat tttaaatttt
     5821 tctccctgac tctctttttt cacttttcaa aatactgcac tattccctct tttattgcct
     5881 gtgccagttt ttcttgataa tccttatccg atagtttctg ttcctcctgc cggtttgaca
     5941 ggaatccaca ttcaactatt acggtaggaa cctttatgtt tcgaagaatt atcagtttac
     6001 tgtcctttac cagtgcttcc cttttattac ttgggtccac tttttcccgc aaagcttttt
     6061 gaatgatttc cgccagttcc ttactctcct gtgacccggg aggaaagaat acctgggcac
     6121 cgtaatactg tgtctggctg aatttgttaa gatgtatgct cacagcaata tcggctccgg
     6181 attcatcaat ccttttttta cggttcatca ggtcctgggc cctcttgttg tcatagcctc
     6241 tggttccttc cctgtagttt aagatgtctt cttccctggt cataataacc ctgtaattct
     6301 ctgcctccaa aagctccttt accctctttg ctataaacag gttgacttct ttttccttaa
     6361 gtccactgta gtcactgact gctcccggat cctctccccc gtgtccggca tcaattacta
     6421 ttgttccttt tacattggcg tttgtaacct caacggcatt aaaattcgaa cctatgttaa
     6481 tgctgataag taaaattgac agcacaaata ccaatgctat taacgctatt ttatcttttc
     6541 tcaatactaa aatcatccgg ccctgccccc ttattcatat ctacaataag catattcaat
     6601 aaagggttgg ccttatacta catgtttaac aaaattttat cccttcagtg acacaaattg
     6661 aaaaaaatgc gaaagagaca taagttacca gaacgccatt cttcattttt tgtgccttat
     6721 atgcctccta cctaaaccgg taccatgaaa aatgttttgc aacaacaacc tgagacttaa
     6781 tgcttacaaa aatatttttt cccgttgttt catcatataa atacccatca tatttatctg
     6841 tttttataag tacaggcttt gaaaaagctt cgacatccgg agtaatctca atgcatggct
     6901 caccgtctct ggcacagatt ttcacatcga attctgcagg atactgtcca cccggaagcc
     6961 agtcattgtt aaactgaatt gtaacgaaac ccacatcaaa tttgccgccc tttttgtcca
     7021 ctgtaatagt agtttcaaat attaccggtt ctgtgaatac agtctcagta ccctttgcat
     7081 ttgacggttt tgccaatgca actgcgggta ttccggttat caagagtgca aacaataacg
     7141 caataatcag tttccttttc ataaaagaac ctccttttat taaagttaat gctctcgaaa
     7201 actcgtattg ctttcgaggt ctctatttca ggagcaagct
//
LOCUS       DAOOJC010000067         7222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_8665_length_7222_cov_4.200363, whole genome shotgun sequence.
ACCESSION   DAOOJC010000067 DAOOJC010000000
VERSION     DAOOJC010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8665_length_7222_cov_4.200363"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..387
                     /locus_tag="PLA01_03015"
     CDS             232..387
                     /locus_tag="PLA01_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HPD00303.1"
                     /translation="MTTINCSSNCIHQQEGKCTLDIVTKNSASVSSDCAFFEEKTAKQ
                     AIEKATG"
     gene            588..1778
                     /locus_tag="PLA01_03020"
     CDS             588..1778
                     /locus_tag="PLA01_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPD00304.1"
                     /translation="MYTEEIKKISSSIINEMVSIRRDLHQNPELGFHEHRTSSIVSGF
                     LKNIGFRVFTNIAKTGVVGILEGSSPGKTIAIRADMDALPITEENDIVFASRNKNIMH
                     ACGHDAHTAIALGTAKILSGMRDKISGNVKFIFQPAEESLGGARLMIDEGALDNPATD
                     AIIALHVSPSLTSGKISVGSGPVMASPAEFDIVIKGKGGHAAQPDKTIDPISIGINII
                     NMFQSIIPKRISVFKTAILSITCFQGGNTYNITPSEAAIKGTVRAFDRETHDEISKIM
                     HSIIASVTGEAGADFSFDYNLGYPPVVNNQEIAELIACASGKILGTENVIINPEPSML
                     AEDFSYYAQKIPGAIFNLGCRSPFDEHSHNLHSSRFTIDEACIAIGMEIMSQCALDFL
                     SINL"
     gene            1885..2103
                     /locus_tag="PLA01_03025"
     CDS             1885..2103
                     /locus_tag="PLA01_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HPD00305.1"
                     /translation="MDRTPLDRLALVLVIIGALNWLLTGLFDYNLVADMFGASSFITR
                     TIYSIVGLAGIYSISLLFRQSEPARDKA"
     gene            complement(2283..2462)
                     /locus_tag="PLA01_03030"
     CDS             complement(2283..2462)
                     /locus_tag="PLA01_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00306.1"
                     /translation="MVVLSNLVYDILENQIKSEIDKNIRDMGVKIRGISIDADTNLNI
                     KVVLVSNEWEHKNIS"
     gene            complement(2566..3294)
                     /locus_tag="PLA01_03035"
     CDS             complement(2566..3294)
                     /locus_tag="PLA01_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPD00307.1"
                     /translation="MSGHSKWANIKHKKEKTDAQRGKIFTKIGREIAIIVKQGGADPE
                     ANSKLKDVIAKAKAANMPNDTITRSIKKAAGDVDSANYEEIVYEGYGPGGVAVIVEAM
                     TDNRNRTAGDIRHFFDKFGGNLGTSGCVSFMFDKKGVILIEKKDGVVEEDLMAHVIEA
                     GVEDFNVEDEYYEIITEPDDFSNVREYLEQKGYEFMSAEVEMVPQTMTNLTDPKHIEN
                     MNKLIENLEDLDDVQNIFHNWDEE"
     gene            3579..4730
                     /locus_tag="PLA01_03040"
     CDS             3579..4730
                     /locus_tag="PLA01_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPD00308.1"
                     /translation="MNIKPDVHTRLKIIVFLLIATIAIVNVGSFLLFSISNDKALTYS
                     KPVLGFPMMQNICHAIIEICLGIIAVIAIYENRNIRKKNRLQIEKLIKQLEKELNEKN
                     ELMENIKSHYDKALKLINQRSEFFYNISHELKTPLSVILGAIQLIDQKYPLEGSDRRK
                     SSKHLCTIKQNCYRLLRLINNVLDISRLENGYIKTNLVNCNIVYLTEEITESVIPFVE
                     QKGLSIEFDTLDEEIITAVDIDKIERIILNLLSNAIKFTNPGGMIYVKVARKDNNVVI
                     HIIDTGIGIPRDMQSKIFDRYSQVSSELTAEFEGSGIGLSIVKSFVELHNGSIEVQSE
                     ENHGSKFTITLPIMVCDKHYNADQVQNNTQNRIIETVRIEFSDIYTMPT"
     gene            complement(4764..5414)
                     /locus_tag="PLA01_03045"
     CDS             complement(4764..5414)
                     /locus_tag="PLA01_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPD00309.1"
                     /translation="MNKEYKTVLNHAVYEIEEKKSRFIASVKPVSAEDEALEFINELK
                     SKYWDATHNVYAYYIGTNNITQRFSDDGEPSGTAGLPVLEVIKRMEVQDLAVVVTRYF
                     GGTLLGASGLIRAYGKSASLGIEAAQVVTKKLCKEITVILEYTMLGKVQSLLISKGFT
                     IKDVIYEQDVKIKVFIPEDEVEAFTNFITETTNAKAVMEIGPKTYITLDSQGKVIL"
     gene            5574..6140
                     /gene="lepB"
                     /locus_tag="PLA01_03050"
     CDS             5574..6140
                     /gene="lepB"
                     /locus_tag="PLA01_03050"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPD00310.1"
                     /translation="MKILKEILSWFTHIAFAVLCGLLITIFIIQPTKVRGSSMESTLH
                     NNDRIIISKLIHTFRLEPNYGDIVIIDKRIERPRSIVDDITDCFKYNAISYFFNKDNE
                     EIFWIKRVIGKPGDKLEFIDGKIYRNGEALDEPYIKEDMIYTKDSPIIVPENCVFVMG
                     DNRNNSTDSRIIGAVPLDHIIGKYVLKF"
     gene            complement(6245..6664)
                     /locus_tag="PLA01_03055"
     CDS             complement(6245..6664)
                     /locus_tag="PLA01_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPD00311.1"
                     /translation="MLVRSYVGGVVFSGDKVLLLRNEKDEWVLPRTVMQDGEIPNEVA
                     VKKIGEGSGIAAEIVCTAGQTNYEFSSVTRQKPFCNKTTWYIMKSLNENLNEVRKKDN
                     KPERICFFKVDKAMDIISSNQDKSLVNLSFKKYREFG"
     gene            complement(6877..7107)
                     /locus_tag="PLA01_03060"
     CDS             complement(6877..7107)
                     /locus_tag="PLA01_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00312.1"
                     /translation="MGKVFVDTIVQFTKEGEKIPLEIVWKDGQRFEVDRVLDIRKAAS
                     LKAGGRGIRYKCRVRGREIYLFLDDDKWFIET"
BASE COUNT         2260 a         1511 c         1222 g         2229 t
ORIGIN      
        1 gcattgtttg aacgaagtga gttttgctcg accccacgga gtatttacag gctctgactt
       61 gcgtaactta agtttctgga acacttttct tcacttttag tggaagaaac attgtgaagc
      121 aagttttagc cgcctgttaa taaataaacc ttttatggga acaataaatt caggtacatt
      181 taatgcttaa ccaacagcta ttgtcataat ctttcgaaca ggagtgatat tatgacaact
      241 attaactgct cgtcaaattg tattcatcag caggaaggca agtgcacact ggatattgtc
      301 acaaaaaact cagcttcagt ctcatccgat tgtgctttct ttgaagaaaa aacagcaaaa
      361 caagctattg agaaagccac aggataaacc ataccgaaaa gcaaaggggc tgtatgcccc
      421 ttaagctttt accatggccg caaaaaccca gctacatatt attttagccg gaaaaaatat
      481 ttaaaaaaga ttgtgaggaa agtggcaaaa cttgttaatg caatgtagaa agggtatggc
      541 ttgcactaat ctgcaaaatg atatatatta aaactcaggt gattaaaatg tatacagaag
      601 aaatcaaaaa aatatcttcc tctataatca atgagatggt ttccataaga agagacctcc
      661 atcaaaatcc tgaattaggg tttcatgaac atagaacctc ttctattgtg tcgggctttc
      721 taaaaaacat cggctttagg gtttttacaa acatagcaaa aaccggcgtt gtgggtattc
      781 ttgaaggaag cagtcccggc aaaacgattg caatcagagc agacatggat gccctgccca
      841 tcactgagga aaacgacatt gtttttgctt cccggaataa gaacattatg catgcctgcg
      901 gtcatgacgc tcatactgcc atagccttgg gtacggcaaa aatactctcc ggcatgagag
      961 acaaaatatc cggcaatgtt aaatttattt ttcagccggc tgaggaaagc ctcggcggag
     1021 caaggctaat gattgacgag ggggccttgg acaatcctgc aaccgatgca ataattgccc
     1081 tgcatgtatc cccttcgctc acatcgggta aaatctccgt aggctccgga cctgttatgg
     1141 cctccccggc agaatttgac atagtcataa aaggaaaagg cggtcatgct gcccaaccgg
     1201 ataaaacaat cgatcctata agcataggca ttaatatcat aaatatgttt caatccatta
     1261 tacccaaaag gatcagtgtc tttaaaaccg caattttatc aataacatgt tttcagggcg
     1321 gcaacaccta taacattact ccgtcagagg ctgctataaa aggcactgtc agagcttttg
     1381 acagagaaac ccacgatgag atatccaaaa tcatgcattc catcatagca tcggtaacag
     1441 gtgaagccgg agcagacttt tccttcgatt acaacctggg ctatcctcct gtggtaaaca
     1501 atcaagaaat tgcagagctt attgcatgtg cttccggtaa aattctcgga acggaaaacg
     1561 tcattataaa tccggagcca tccatgcttg cggaagattt ttcatattat gcacaaaaaa
     1621 tacctggggc catttttaac ctgggctgca gaagtccttt tgatgaacat tcccacaatc
     1681 ttcattccag ccggttcaca attgatgaag catgtatcgc tataggtatg gaaataatgt
     1741 ctcaatgtgc actggatttt ttaagcatta atttgtaaat cgttccataa ttttattgcg
     1801 gcaccaactc atattttgct atgaattttt aattttaata aggtaaataa tacgaatata
     1861 aataaataaa aaggagttgt cactatggat agaacacctt tggacaggct tgctctggtt
     1921 ttagttataa tcggagcatt aaattggctc ctgactggat tattcgatta taacctcgta
     1981 gcagatatgt ttggagcaag ctcatttata acaagaacaa tatactccat tgtaggtctt
     2041 gcaggaatat acagtatcag cctgttattc aggcaatcag aacctgcaag ggataaagct
     2101 tgattaaaaa tgttaaaagc tcaggggcat ccagcctctg agtttttaga gctttcattt
     2161 tattgaagct gttaaggggg cgtcaagccc ccattgcttt tcagtcattt catttcgtga
     2221 tatccgacta attaaaatcg aaggcggcac caaacccctt cgattcttat aaacataaat
     2281 tattaactga tatttttatg ctcccattca tttgatacca gaacaacctt gatattcaga
     2341 tttgtatccg catcaatcga aataccgcgt attttcactc ccatatcccg aatgttttta
     2401 tcaatctcac tttttatttg attttcaaga atatcataaa ccaaattgct caaaactacc
     2461 actcctccct gaacatataa ataatatatc agatttttcg gctattgtta atacccgtgg
     2521 gaaattattt gaacaaggtc cggttttgag ccatatttta agcctctatt cctcatccca
     2581 gttatgaaaa atattttgta catcgtcaag gtcttccaga ttttcaataa gcttattcat
     2641 attctcaata tgttttgggt ctgtgagatt ggtcatggtt tgcggaacca tctcaacctc
     2701 tgccgacata aactcatacc ccttttgttc aaggtattct ctcacatttg aaaaatcatc
     2761 gggttcggta atgatttcat agtattcatc ctcgacatta aagtcctcaa cacccgcctc
     2821 aatcacgtgt gccattaaat cttcctcaac tacgccgtct ttcttttcaa tcagtattac
     2881 acccttttta tcaaacataa aggaaacaca acccgatgtg ccgagattac ctccaaattt
     2941 atcaaagaag tgcctgatat cgccggctgt cctgttccgg ttatccgtca tggcttcaac
     3001 aataacggct acacctcccg gtccataacc ttcgtatact atctcctcat aattggcaga
     3061 atcaacatct ccggcagctt tctttatgct tctggtaata gtatcattag gcatattcgc
     3121 agctttagcc ttggcaataa catccttcag ctttgaatta gcttcagggt ctgccccccc
     3181 ttgttttaca attatcgcaa tttcccttcc gatttttgta aatatttttc ccctttgagc
     3241 atcggttttt tcctttttat gcttaatatt agcccatttt gagtgtccgg acataaaata
     3301 cctccttaca gttatatata aataatagac ttatcctctt tagattataa tattaaacgg
     3361 caaatttata aagtttttta aataaaaata tgccggttca tttcaaatgt tgccatatcg
     3421 cttaaaacac tctgattttt taatcatgta tctaaaaaat gtatttacat atttgaatat
     3481 ttcctataaa atatctatgg tattttatgt attgtgcact atataatcct attaattaaa
     3541 atcatttata attttttgtt ataaaaaggg ttggtgctat gaatattaag cctgatgttc
     3601 atactcgtct gaaaattata gtatttttac tgatagccac tatagccatt gtaaatgtcg
     3661 gctctttttt acttttcagt ataagcaatg ataaggcttt gacatattcc aaaccggttc
     3721 ttggctttcc catgatgcaa aacatttgtc acgcaataat agaaatatgt ttgggaatta
     3781 ttgcagtcat tgcaatatat gagaacagaa acattcgtaa aaaaaacagg cttcaaattg
     3841 aaaaactgat aaaacagctt gaaaaggaac ttaatgagaa aaatgaactt atggagaata
     3901 ttaaatccca ttatgataaa gccttaaaac taatcaacca aaggtcagag ttcttttata
     3961 acatctctca cgagttaaaa acacccctca gcgttatttt aggtgcaata cagctcatcg
     4021 accaaaagta ccctcttgaa ggcagtgaca gaaggaaatc cagcaaacac ctgtgtacca
     4081 taaaacagaa ctgttacaga cttctaagat tgataaacaa tgttctcgat atcagccgtc
     4141 tcgaaaacgg atatattaaa accaatcttg taaattgcaa catcgtgtat ctgactgagg
     4201 aaataaccga atccgttatc ccctttgtgg aacaaaaggg actatccata gaattcgaca
     4261 cccttgacga ggaaataata actgctgtgg atattgataa aatcgaaagg ataattctga
     4321 acctgttatc caatgccatc aagtttacaa accccggcgg aatgatatat gttaaagtag
     4381 cccgcaagga taataatgtg gtcattcata taatagacac cggtattggg attccccggg
     4441 acatgcagtc aaaaattttt gaccgctaca gtcaggtaag cagcgaactc acagcagaat
     4501 ttgaaggaag cggtattggt ctctccatag taaaatcctt tgttgagctt cacaatggaa
     4561 gcatagaggt tcaaagtgag gaaaaccacg gaagtaaatt tacaataaca ctccccatta
     4621 tggtttgtga taagcactat aacgctgacc aagtccaaaa caatacccag aacagaatca
     4681 tcgaaaccgt tagaattgag ttttccgaca tatacacaat gcctacataa caaattccgc
     4741 cacagctttt accgcaattt aagctacaat attacttttc cctgcgagtc cagtgttata
     4801 taggttttag gtccgatctc cataactgcc tttgcatttg tagtttcagt aatgaagttt
     4861 gtaaaggcct ctacctcatc ctccggaata aatactttta tttttacatc ctgctcatat
     4921 atgacatcct ttatcgtaaa accttttgaa atcaggagac tttgaacctt tcccagcatg
     4981 gtgtactcta gaatcaccgt tatctccttg caaagctttt ttgtcacaac ctgtgcggcc
     5041 tctatcccca acgaagcact ctttccgtaa gcacgtataa gcccggaagc acctaaaaga
     5101 gtccctccaa agtacctcgt tacaaccact gcaaggtcct gtacttccat tctctttatg
     5161 acctcaagca cgggaagtcc tgctgttccg gaaggctcgc catcatcgct gaatctctgt
     5221 gtaatattgt ttgtgcctat ataataagca taaacattat gggtagcatc ccagtacttt
     5281 gacttcagct catttataaa ctccagtgcc tcatcctccg ccgacacagg ctttactgat
     5341 gcaatgaacc tcgatttttt ctcctcaatc tcatatacgg catggtttaa gacggtctta
     5401 tattctttat tcaaaattac cacctttaaa ttattttttc gtacaggtct attttaacac
     5461 ttttttatca atattcaaaa attattgttc cgggtggcat agtccccgtc aaatatgata
     5521 taattatttt gaaaagaata tcaatgcaac tcacagtagg ggaggaaaat agcgtgaaaa
     5581 tcctgaaaga aattctaagc tggtttactc atatagcgtt tgcagttctg tgcggccttc
     5641 ttattaccat ctttatcata caacctacca aggttagggg aagctccatg gagtctacat
     5701 tgcataataa cgaccgcatc ataatcagca agctgataca tacctttagg ttagagccca
     5761 actatggcga tattgttatc attgacaagc gtatcgaaag gccacgctct atcgttgacg
     5821 atataaccga ttgcttcaag tataatgcca tctcatattt tttcaacaag gacaatgaag
     5881 aaattttctg gatcaaaagg gtgatcggca agccgggaga caagcttgag tttatcgacg
     5941 ggaaaatata tagaaacggc gaagccttag atgaaccgta tattaaggaa gatatgattt
     6001 atactaaaga ttcacctata attgtccccg aaaactgcgt ttttgtaatg ggagacaaca
     6061 ggaacaacag caccgacagc agaattatag gtgccgttcc ccttgaccat ataataggca
     6121 aatatgtttt aaaattttag ataatttaac tttgctgtaa gcaaaggcgg attaaataat
     6181 tgatacactt ttataaccgg aaaaaagggc attaagccct tttttattgt cttatatatt
     6241 gcaatcatcc aaattctctg tattttttga aggataaatt gaccagtgac ttatcctgat
     6301 tcgaactgat aatatccatg gccttatcta ctttaaagaa acaaatccgc tccggtttat
     6361 tatctttttt tctcacttcg ttcaagtttt cattaaggga tttcataata taccatgtag
     6421 ttttgttaca gaagggtttt tgacgtgtta cacttgaaaa ttcataattt gtctgacctg
     6481 ccgtacacac aatttcggct gctatgccgg aaccttctcc tatctttttt actgccacct
     6541 cgtttggaat ttccccgtcc tgcataactg ttcttgggag tacccattca tccttctcat
     6601 ttctcagaag aagaactttg tctccggaga acactactcc acccacatag cttcggacta
     6661 gcataataaa aacccctttc attgtcaatt tttttaccct tatcaagggt tatataaaaa
     6721 cattaggaat ctgaacgata agaacacggc ttgttccgta actgacaaaa ttttattata
     6781 tattattata atactataaa ggtagtcttt attcaaacta tagggaaata aaagggttca
     6841 tgaaataaat aaatgtgaaa aaaacattcc ggaaccttaa gtttcgataa accatttgtc
     6901 atcgtctaga aaaagatata tttctcttcc ccttaccctg catttatatc ttatacccct
     6961 tcctcctgcc tttaaggatg cagccttcct tatatcaagc accctgtcca cctcgaatct
     7021 ctgtccgtct ttccacacta tttccaaagg aatcttttct ccttctttag taaactgtac
     7081 aatagtatca acaaacacct ttcccatgat ttcaagcacc gccttttcga ataagataaa
     7141 gaaatactgc tgttttcagt tatcttcagc tgttcctgaa ataagaaacc gggaaaatag
     7201 tgtggtcatc ggcaggattg gc
//
LOCUS       DAOOJC010000068         7137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_8785_length_7137_cov_3.438153, whole genome shotgun sequence.
ACCESSION   DAOOJC010000068 DAOOJC010000000
VERSION     DAOOJC010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8785_length_7137_cov_3.438153"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PLA01_03065"
     CDS             complement(<1..543)
                     /locus_tag="PLA01_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00313.1"
                     /translation="MIRKSKFLTFILSWVPGLGHLYLGFNVRGGAFFFAELAVVILTI
                     AFEAGFGSNKAPILMLLIPVIWLGAIVDSMVLADKYNWLVQIGKNHSEIFESVEIEKQ
                     NKKIIAMCLSIIPGAGHMYLGLQRQGVQLMTMFFLGIFLTDWLRVSLFMFVIPVIWFY
                     SLFDVMYKATGINIKEDKDIL"
     gene            complement(658..1137)
                     /locus_tag="PLA01_03070"
     CDS             complement(658..1137)
                     /locus_tag="PLA01_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00314.1"
                     /translation="MEHFDTHKLMLYVNGLAEEDMKLDIENHLQNCDECLEKYIEAIE
                     YKNVHEASALISPEFCSRVMEQITQGEEKIGKIRERRIQNKYQAFMYYVAAACITLIF
                     MASGVFQQIFGSVSNVTSGIEESPGSIEKMVPSGWTDRLVEGTSDVIDVLFGRGYSE"
     gene            complement(1142..1684)
                     /locus_tag="PLA01_03075"
     CDS             complement(1142..1684)
                     /locus_tag="PLA01_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPD00315.1"
                     /translation="MEPEELLIKRVINGDQEAFRSIVEKYQNLVFAICFNITGQRQEA
                     ENLAQETFLQVYRSLPRYRNQGFKAWIGKIAVNKAIDWKRKSKAENEKKLLYIDDIRD
                     LQSDERSLQEQIIMNEDRKRILEICESLPEKYSTVLKKYYIQSKTYSQISEEEGISVR
                     TVETRLYRAKQEIRKKWKEG"
     gene            complement(1886..2704)
                     /locus_tag="PLA01_03080"
     CDS             complement(1886..2704)
                     /locus_tag="PLA01_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPD00316.1"
                     /translation="MQRLLCIILVWILILSFCVGCSDSKKYGFGEDDSCPASSIALTE
                     EEAAQITDKMPICLYFANEDNSKLKLEIRYIPMSEAEKSVNHLAGIIVNELINGPKVA
                     GMKATIPEGTKLKSEIKIDGDVATVNFSKEFKDNHPGGKAEERMTIYSVVNSLTELKE
                     ISKVKFLIEGKSTAEFKGNFKFDAGFPRCAELISTKAEPVGMGSGGQNNADIDDQSVG
                     KNDAFDTVNNGEAQESDVGVYNEDDVFGEAPNEDEEWQESFDEEYEETYIEYLE"
     gene            complement(2786..3481)
                     /gene="deoC"
                     /locus_tag="PLA01_03085"
     CDS             complement(2786..3481)
                     /gene="deoC"
                     /locus_tag="PLA01_03085"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPD00317.1"
                     /translation="METFWDRRTVVSMIDHAVLKPDATDGEVRDQCMLAVKYSVASVC
                     VKPCHVALAHEILKGSNVKVSTVIGFPHGSTTTQCKIHEAVEAIENGAVELDMVMNIG
                     KLLSKDYDYVKKEIETIVTAAHEKGVEVKVIIETALLTDEQKAVASKLSKEAGADYLK
                     TSTGFNGRGATLEDIRIMREAAGPDMKLKASGGIKTFDQAVEFIKAGCVRLGTSSTVA
                     VAEEGTEESIKGV"
     gene            complement(3615..3842)
                     /locus_tag="PLA01_03090"
     CDS             complement(3615..3842)
                     /locus_tag="PLA01_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxx-star family glutaredoxin-like
                     (seleno)protein"
                     /protein_id="HPD00318.1"
                     /translation="MEVKVYSTPTCPYCTMAKKYLDSKNIRYEEYDVSNDRNAATRMI
                     QMSGQRGVPVLDIDGNIVIGFDKERIDSLIE"
     gene            complement(3916..4788)
                     /locus_tag="PLA01_03095"
     CDS             complement(3916..4788)
                     /locus_tag="PLA01_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPD00319.1"
                     /translation="MYDVIIIGKGPAGISASLYTARANLKTLVIGKGDSALKKAEKIE
                     NYYGFSQPIRGEDLLNEGEKQAARLGVQIFENEVISIEKNDYFEVLTPDATYVSKSVL
                     IATGQPLKKVRIENLELFEGKGVSYCTTCDGFFYNNLKVGVVGYKDFVVHEAVELEAY
                     TKDITIYTNGKQLELSEQYKETSKRFKINTKEIYRIDGGDFLQKIFFKDGTSEDIDGL
                     FIAYESPSSVDFARKLGILVDENSIVVDINQQTNIDGLFAAGDCTGGFKQISTAVGQG
                     ALAGRKIIEYVKSL"
     gene            complement(4901..5866)
                     /gene="trxB"
                     /locus_tag="PLA01_03100"
     CDS             complement(4901..5866)
                     /gene="trxB"
                     /locus_tag="PLA01_03100"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPD00320.1"
                     /translation="MGGACQRKEREKVYCDIAIIGGGPAGLSAAIYSARSRLYTVIIE
                     EGLVGGQVSTTFHVANYPGTNGVVRGMELMENMKRQAIGFGTQIDELKEISEISLEGE
                     EKHISTADTDYYAKAVIVATGTAPRRLPADGENEFRGRGVHYCATCDGALYHDAKVLV
                     VGGGNSAVEEAVFLTRYAKHVTIVHQSDRFSASKGAQDEVFKNPNISVVWNSEIRKIN
                     GDNLARSVIIENLKTGEKKEMEADGIFVYIGMQPKTEIFKGKINMDESGHIITDEDMV
                     TNIPGVFAAGDVRSKKVRQIATAVGDGVIAGIMAERLISGKQGLF"
     gene            complement(5900..6190)
                     /locus_tag="PLA01_03105"
                     /pseudo
     CDS             complement(5900..6190)
                     /locus_tag="PLA01_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
     gene            6863..>7137
                     /locus_tag="PLA01_03110"
     CDS             6863..>7137
                     /locus_tag="PLA01_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane trafficking protein"
                     /protein_id="HPD00321.1"
                     /translation="MSDFSKKISELFGKMDEKMLQKKINTAIDMLQNGDIDDLAKKLN
                     KIDKDELMQKINEFDESKLEKLNIDKDEIREKIKDTDLKNLSQLLGD"
BASE COUNT         1952 a         1746 c         1075 g         2364 t
ORIGIN      
        1 cagaatatcc ttgtcctcct taatattgat gcctgtcgcc ttgtacatga catcaaacaa
       61 actgtagaac catattaccg gaatcacgaa cataaacagg cttactctca accagtcagt
      121 aaggaatatc ccgaggaaaa acatagtcat aagctgtact ccctgtctct gaaggccaag
      181 gtacatgtgt cctgcccccg gtattatcga taaacacatt gcaataattt ttttattttg
      241 tttttcaatc tccacactct cgaaaatttc gctgtggttc tttccaattt gaacaagcca
      301 gttgtatttg tccgccaata ccatgctgtc aactatggcc cccagccaaa taaccggaat
      361 taaaagcatc aaaattggtg ccttgttact tccaaagcct gcttcaaagg caatcgtaag
      421 aattacaact gccagttctg caaagaaaaa cgcacctccc cgaacattaa acccaagata
      481 taaatgtcca agtcccggta cccaggataa aataaaggtc aaaaacttgc tttttctgat
      541 cataaaattc ctctcctttg atttattctg cctaaaaccg ttttccaact ataaattcaa
      601 accgttaaaa gcacattttt caatgtgata caatttcttt attcagtaaa gcttctatta
      661 ttcactgtaa cctcttccga agagaacatc aatcacatcc gatgtaccct caacaagccg
      721 gtcagtccag ccgcttggca ccatcttttc tatactcccc ggagattctt ctatgccgga
      781 tgtcacgttc gaaacactgc cgaaaatctg ctgaaatact ccgctcgcca taaatatcaa
      841 tgtaatacac gctgcagcta catagtacat aaaagcctga tacttatttt gaatccttct
      901 ttctcttatt tttccgattt tctcttctcc ttgtgttatt tgttccataa ccctgctgca
      961 aaattcagga gagataagag cggaagcttc atgaacgttc ttgtactcga tggcttcaat
     1021 atatttttcc agacactcgt cacagttttg taaatggttc tctatgtcca gtttcatgtc
     1081 ttcttccgca agtccattga catataacat cagtttgtgt gtgtcaaaat gttccatacc
     1141 attagccctc cttccatttc tttcttatct cttgttttgc tctataaagc cttgtctcga
     1201 cagttcgcac actgataccc tcttcttctg aaatctggct gtaagtcttt gattgaatat
     1261 agtatttttt cagaacagta ctatattttt ccggcagact ctcacagatt tccaaaatcc
     1321 tttttcggtc ttcattcatt ataatctgct cctgaagact tctttcgtca ctttgaaggt
     1381 ctcttatatc atcaatatac aacagctttt tttcattctc cgcctttgac tttctcttcc
     1441 aatctatcgc cttattgacg gctatttttc caatccatgc tttaaaacct tggttccggt
     1501 accggggaag ggagcggtat acctgtaaaa aggtttcctg agccaggttt tcggcttcct
     1561 gtctttgccc tgtaatatta aaacagattg caaacaccaa attctgatac ttctcaacta
     1621 ttgacctgaa tgcttcctga tcaccgttta ttacgcgttt tataagcagt tcctcaggtt
     1681 ccatggctcc cctccttccc ttttttaatt catatctaca ttatcataga cgtaattaat
     1741 tttaaaaccc cttcaatttt tttaaaaaat aattgcatta gtgagatatg aaatatcaat
     1801 acacttttag ggaataaaaa aaacagggaa tctttaatct atcccctgct ttatggcctt
     1861 cattaacccc tgttttaatt tttcattatt ccaaatactc tatatatgtt tcttcatact
     1921 cctcatcaaa agactcctgc cattcttcat cctcattggg agcctctccg aatacatcat
     1981 cctcattata aacccctaca tcggattcct gggcttcacc gttatttacc gtatcaaaag
     2041 catcattctt accgacgctt tgatcgtcta tatcagcgtt gttctggcct ccgcttccca
     2101 ttcccacagg ctctgcttta gtgcttatca actccgcaca ccttggaaag ccggcatcaa
     2161 atttgaagtt ccccttaaat tcagcagttg atttaccctc tattaaaaac ttcaccttgc
     2221 ttatttcctt aagctctgtc aaagagttta caaccgagta aatggtcatt ctttcttctg
     2281 ccttccctcc cggatggttg tctttgaact cttttgaaaa attaactgtt gcaacatctc
     2341 catcgatttt tatttccgac ttaagttttg ttccttccgg tatggtagcc ttcatccccg
     2401 caacctttgg accgttaatc agttcgttga caattatccc ggcaaggtga tttacacttt
     2461 tttcagcttc actcatcggt atgtatctta tctcaagctt tagcttggaa ttgtcttcat
     2521 tcgcaaaata aaggcagatt ggcattttgt cggtaatctg ggctgcttcc tcctccgtca
     2581 gagcaatgct gctggcagga catgaatcat cttctccaaa tccatatttt ttgctatcgg
     2641 aacaccccac acaaaatgat aatatcaaaa tccataccag aataatacat aaaagtcttt
     2701 gcatcaaccg gacacctcac aataaattct ttatagctta tttttatgcc ggttggactt
     2761 ttagtattct tatccaaata aattttcaaa cccctttaat gctttcctct gttccttcct
     2821 ctgcaacagc aactgttgaa cttgttccca gcctgacaca gcctgccttt ataaactcca
     2881 ctgcctggtc aaaggttttt attcccccgg atgctttcag cttcatatcc gggcctgctg
     2941 cttccctcat tattcgtatg tcttcaaggg tagcacccct gccgttaaac cctgtggatg
     3001 ttttcaaata atccgctccg gcttctttgg aaagcttgct ggccacggcc ttttgttcat
     3061 ccgtcaatag ggcagtctct attataacct tcacctcaac acctttttca tgtgctgccg
     3121 tcacaatggt ttctatctct ttttttacat agtcatagtc ctttgacaaa agcttcccta
     3181 tgttcattac catatccagt tccacagcac cgttttcgat ggcctccact gcctcatgaa
     3241 tcttgcattg agtggttgtt gaaccatggg gaaatccaat gactgtgcta acctttacat
     3301 tagaaccctt caaaatttca tgggccaaag ctacatggca tggctttacg caaaccgaag
     3361 caacggagta ttttaccgca agcatgcact gatccctgac ttcaccatcc gtagcatccg
     3421 gttttaaaac cgcatgatca atcattgaaa ccactgttct tctatcccaa aaagtttcca
     3481 taccttcctc cccttttcat ccttaacctt ataaaaaaac ccggtgacaa aatcacaggg
     3541 ttcgacacac aatcaaaacg aaattattcc atcggcaaaa ttttcattat tatattagtg
     3601 cttttaatat attactattc gatcagactg tcaattcttt ctttatcaaa gccaataact
     3661 atgttgccgt caatatccaa taccggcaca cccctttgcc ctgacatttg tatcatacgg
     3721 gttgctgcat ttctatcgtt tgaaacatca tattcctcgt atctaatatt ctttgaatcg
     3781 agatattttt ttgccatagt gcaatatggg catgttggcg tggaataaac cttaacttcc
     3841 atagtatctc ctcctttatt attacaatta gtaaaaacca aaggggcggc cttctgtttg
     3901 tcaaagctgc aatcattaaa ggctcttaac atattcaata attttcctgc ctgctaaagc
     3961 accttgtccc acagctgttg aaatctgctt gaaaccacct gtacaatccc ctgcagcaaa
     4021 caatccgtct atattagtct gctggttgat gtcgacaaca atggagtttt catccacaag
     4081 gatcccaagc ttccttgcaa aatcaacgct cgaaggactc tcataagcaa taaacagccc
     4141 atctatgtct tcgctcgtac cgtccttaaa aaaaatcttc tgaaggaaat ctcccccgtc
     4201 aattctgtaa atctctttgg tatttatttt gaatcttttt gatgtttctt tgtactgttc
     4261 cgacagctca agctgttttc cgtttgtata tatcgttatg tcctttgtat atgcttccag
     4321 ttcaaccgct tcatgcacca caaagtcttt atatcctaca acaccgactt taagattgtt
     4381 gtaaaaaaat ccgtcacagg tggtgcaata gctcactcct ttcccttcga aaagctccag
     4441 attctctatc ctgacctttt tcaacggctg ccccgtggct attagaacag acttggaaac
     4501 ataagtagcg tccggggtca aaacttcaaa ataatcgttt ttctcaatgc ttattacttc
     4561 attctcaaaa atctgtaccc cgagccttgc cgcctgcttc tcaccctcat tcaaaagatc
     4621 ttctcctctt atgggctgtg aaaagccata gtagttttct attttttctg ctttcttaag
     4681 ggcactgtcg cctttaccta ttaccagtgt ctttaaattt gcccttgcgg tatataaaga
     4741 agcagaaata cctgccgggc cctttccaat tattataaca tcgtacataa tcaattttcc
     4801 tcacttagtt tatacttccc cttaatttta tattctaaac agaaattttt caataacccg
     4861 gtcagggtgt attatcaaaa tgcgaacaaa acctatcaaa ttaaaatagc ccctgctttc
     4921 cgcttataag cctttccgcc atgattcccg ctattacccc gtcacccaca gcggtagcga
     4981 tctgtctgac ttttttactc ctgacatctc ccgcggcgaa aaccccggga atattggtaa
     5041 ccatgtcttc atccgtaata atatgtccac tttcatccat attgattttc cccttgaaga
     5101 tttccgtctt cggttgcatt ccaatgtata caaatattcc atcagcctcc atttcttttt
     5161 tctccccggt cttaagattt tcaataatta cacttcttgc taaattatcg ccatttattt
     5221 ttcttatctc ggaattccaa accacactga tgttcggatt cttgaaaacc tcatcctgtg
     5281 caccctttga tgcactaaac cgatccgact gatgaacgat agttacatgt tttgcgtacc
     5341 gggttaaaaa tactgcttcc tcaaccgcag agttgccgcc gcccactacc aaaacctttg
     5401 catcatgata caatgctccg tcacatgtgg cacagtagtg cactcctctg cccctgaatt
     5461 cattttcccc atcggcagga agccttctcg gtgcagtacc ggttgcaact attacagcct
     5521 ttgcataata gtcggtatct gcagtggata tgtgcttttc ttccccctca aggctaattt
     5581 cggatatttc cttaagctca tctatctgcg taccaaagcc aatggcctgc cttttcatat
     5641 tttccatcag ttccattccc ctaaccaccc cattggtacc gggatagttt gcaacatgaa
     5701 aggtggtaga cacctgacct cccaccagtc cttcctcaat gattaccgta taaagtctgg
     5761 accttgcaga gtaaatagct gcagacagtc ccgcaggtcc tcctccgatt attgcaatgt
     5821 cgcaatatac cttttccctt tctttccttt ggcaagcacc tccaataaca gtctctatct
     5881 tacctgcaat ttctaattta ccgatatagc cgtttaatct tgagcagacc tcacgcccgt
     5941 ctttaaaaaa caaaacagtg ggactgtttt tgatgcccat gctttgtgac aactgccggt
     6001 tctcctgccg ttgaattttg acaaaccgaa tattttcacc gtagatttgg gccattcttt
     6061 caaacatcgg ttcaaaatag ccacagtggg gacagtcttc cgaatagaaa tataccacca
     6121 ctccaggctc gctttttaac acgtcattct caaatttttt ctcattggaa taaataattc
     6181 cgcttctcat aatatacctc ccgggaatca ttcattttaa atacctattt tccatatttc
     6241 ccttgtctgg ggttaattac acaaaaaaat ttcacagttc tgcaacattt atgcgaccaa
     6301 tgaatattat gttaatatac accattataa cacttcagtt caaagaaaac ttctcgtttg
     6361 cagaaaactt tcttgcatgg gaagtgtttc aacgaaatca gctgcaacaa caaatcagga
     6421 ggtgtaactt gttatgtgtg gagaaattaa taaaagcaat tccagtattt tgttctttat
     6481 actggtcttt ctattattgt tttatgacag cagaccgata ggaggcatca aacacactgt
     6541 tgatgggaac gatgattctt tgctgttttt catccttgtg ttccttaaac tcttttattg
     6601 ctaagcattc gagtgcatat tgttctaatg tataaactat taggtgcata tttttcattt
     6661 tatttattat ctgtcttcca atctttcctc cctttatttt tttttgctat gaaggttcta
     6721 ttttcagtaa tttataaata caaattaaat tagaaaacaa ttccttgatc tttttgtgca
     6781 ttcttaacat taccaatcat ttaaatatgt gatgttaaca agcacagggt tatacaaata
     6841 ttaattgaaa ggatcgtgtt ttatgagtga tttcagcaag aaaatatctg aactgttcgg
     6901 aaaaatggat gagaagatgc tgcagaaaaa aatcaataca gcaatagata tgctgcaaaa
     6961 cggggatatt gacgatttgg caaaaaagct caacaaaata gacaaggacg aattaatgca
     7021 aaaaattaat gaatttgatg aatcaaagct cgaaaaacta aacatcgata aagatgaaat
     7081 ccgggaaaaa attaaagaca ccgacctgaa aaacctttca cagcttttag gggatca
//
LOCUS       DAOOJC010000069         7099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_8847_length_7099_cov_3.784497, whole genome shotgun sequence.
ACCESSION   DAOOJC010000069 DAOOJC010000000
VERSION     DAOOJC010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8847_length_7099_cov_3.784497"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1795)
                     /locus_tag="PLA01_03115"
     CDS             complement(<1..1795)
                     /locus_tag="PLA01_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HPD00322.1"
                     /translation="MESIFSVVLMTSLYGTVVGIVLILIKNLLKNRLSPKWHYLIWIV
                     LILKLLIPFGPESAVSLFNIIPVTYEIGSIDDSAEIYNERTILLPTEKEISNTENYEI
                     PTQQNFNNNTVGNTDIIKSNAKKTIAFIWFFGALFMLLWLTYTYLSFYKKLQKETYDI
                     DQRVITVFEKCKKRIGVKKNLCLTVSNTVNIPSLFGVIKPKIILPPETAKLSDKEIEY
                     VLLHELAHYKRKDILINLLLLGIQVVHWFNPIMWYCFKCIRQDMEAATDERVLSILEH
                     TEHKDYGSALIAIIENFTRPLLAPRLLAPRLLGMVNDRKSMERRIRMIKMAEIFKRKR
                     NISIIVGIACILVLGGVLLTNRISAKIKDENNNVNKNIDSINNAGEQNIQEDTQKPKS
                     LEEAISQAIKAQGKSFMTGEFLTEGHIILDKEEKDGEVKVYTLSSVGWFSFEDGIFRK
                     ISGSGTIPTVMTFAVSENGGFTLIEYREPEDGEGYMESIYEMFPEKLYDAVRSSHERY
                     PELLKQQEEQAQSYLKSIGRKADVDSKYVNKTQVDIDYEARGILFAIYNKFDTFLNTC
                     PDWIGSKEYVEDGIRYIYETLQSKTVEGYDLI"
     gene            complement(1798..2172)
                     /locus_tag="PLA01_03120"
     CDS             complement(1798..2172)
                     /locus_tag="PLA01_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HPD00323.1"
                     /translation="MKAESQITESELEVMKILWEFGKATSSQIIEKLTETTEWKPKTI
                     QTLITRLVTKGAVGAEKINSKSFIYYPIVSEEQYKSYANKSFLQKLYNGSVNLMLASF
                     VKDQKLSKKDLEFLKQLLDEEE"
     gene            3030..3503
                     /locus_tag="PLA01_03125"
     CDS             3030..3503
                     /locus_tag="PLA01_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPD00324.1"
                     /translation="MGFFDFLFNNGSGIKTDNNIGPTQSRPLDFQPYNEEFSMDMTTE
                     PYGNNMFESNNTFSNNGVELQDARNTLRLVYSGILAKNDADEVYAVVGYGNNLAWEDT
                     ESYPMHKISHDKFELMLPVKRPGNINIAFKDGTENWDNNSGMNYSFKNYVYEGSQ"
     gene            complement(3558..3848)
                     /gene="yajC"
                     /locus_tag="PLA01_03130"
     CDS             complement(3558..3848)
                     /gene="yajC"
                     /locus_tag="PLA01_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPD00325.1"
                     /translation="MPQEYMNLIGTLIIPIGFIGAMYLFVILPQKKRDKQHSQMLDAL
                     EVGDNVVTTSGIIGKIVNIKDDEITIETSVEKTKIKFVKSAIGSVEKESKEA"
     gene            complement(3906..5024)
                     /gene="tgt"
                     /locus_tag="PLA01_03135"
     CDS             complement(3906..5024)
                     /gene="tgt"
                     /locus_tag="PLA01_03135"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPD00326.1"
                     /translation="MGAIRYELIKKCKQTGARLGRVHTPHGSFDTPAFMPVGTQATVK
                     GMSPDELKDIEAQIILSNTYHLHMRPGEDIVKEAGGLHGFMNWDRPILTDSGGFQVFS
                     LSDLRDIKEEGVTFKSHIDGSKHFITPEMATRIQNDLGADIIMAFDECIPYPAEYDYA
                     KKSLERTTRWAKRCKETHRNPDTQALFGIVQGGMYKDLREQSAYELLELDFPGYAIGG
                     LSVGEPAEEMYAMLEHTVPLLPEDRPRYLMGVGSPDYLIEGAIRGIDMFDCVLPTRIG
                     RNGTVMTGNGRVIIRDAAYARDYSSLDPECDCYTCRNFSRAYLRHLLKAGEVLGIRLT
                     TWHNLRFLINLMKNVRQAIMEDRLGDFRNEFFNKFGYK"
     gene            complement(5132..6157)
                     /gene="queA"
                     /locus_tag="PLA01_03140"
     CDS             complement(5132..6157)
                     /gene="queA"
                     /locus_tag="PLA01_03140"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPD00327.1"
                     /translation="MKVSDFNYDLPQELIAQEPIPNRQMSRLLVLDKNTGSIEHRIFK
                     DVLEYVNEGDCLVLNDTRVIPARLLGEKEGTGGKIEFVLLKKIENDIWEVILKPGKRA
                     KPGSKFVFGDGVLKAEVVEIVEEGNRLVRFEYDGIFEEVLDRVGIVPLPPYITKKLDD
                     AERYQTVYSKYKGSAAAPTAGLHFTKELLDEIRNKGVDIAYVTLHVGLGTFRPVKVED
                     VLEHKMHSEYYRINEEDCRKINSAKEKGKRVIAVGTTSCRVLETVGDDSGRMNPESGW
                     TDIFIYPGYRFKVVDALITNFHLPESTLIMLVSAFAGRDRIMEAYNTAVRERYRFFSF
                     GDAMFIK"
     gene            complement(6432..>7099)
                     /locus_tag="PLA01_03145"
     CDS             complement(6432..>7099)
                     /locus_tag="PLA01_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPD00328.1"
                     /translation="TYNGKPASVFYFSSSGGRTEDVKNVWGSDGYPYLISVEDKYESG
                     DSWHYNWEVSFTAQKIKQIMIDRGFNLGDILGIFITKRSEAGRATEVVVKGSKDERIY
                     TNGSTRSFLSLDSQWYEITTDADTLVMGVEGEPVKTQLGGKKVMTASGLKTISASGNS
                     VSVVSSGSIKKIPIIPTIYTIIGKGWGHSVGMSQEGAKGMAKAGFTYKEILAHYFAGT
                     KVE"
BASE COUNT         2049 a         1616 c         1059 g         2375 t
ORIGIN      
        1 taattagatc atatccttca acggttttac tttgtaaagt ctcatatata taccttatcc
       61 cgtcttcaac atactccttt gagcctatcc agtcaggaca agtattgaga aaagtatcaa
      121 atttattgta tatggcaaaa agaatacctc tggcttcata atcaatatcg acctgagttt
      181 tatttacata tttcgaatca acatctgctt ttctccctat actcttaaga taactttgtg
      241 cctgctcttc ctgctgcttt aaaagttccg gatatctctc atgggatgac ctaaccgcat
      301 catacaattt ttccggaaac atctcgtaaa ttgattccat ataaccctct ccgtcttccg
      361 gttctctgta ctcaataaga gtaaacccac cgttttcgct gactgcaaag gtcataaccg
      421 ttggaatcgt accgctgccg ctaatctttc taaatatacc gtcttcaaag ctaaaccatc
      481 ctacactgga taaagtatat actttcactt ctccatcctt ttcctcttta tccaggatga
      541 tatgtccctc agttaaaaac tcgcccgtca tgaaagattt gccttgtgct tttattgctt
      601 ggcttatagc ctcctcaagg cttttgggct tttgagtatc ctcttgtatg ttttgctcac
      661 ctgcgttgtt tatagaatca atgtttttat ttacattatt gttctcatcc tttatttttg
      721 cacttatcct attggtaagg agtactcccc ccagaaccaa tatacaagct atccccacaa
      781 ttattgaaat atttcttttt cttttaaaaa tttccgccat ctttatcatt ctaatcctcc
      841 tctccatact cttcctgtca ttaaccatac caagcaatct tggagcaagc aatcttggag
      901 caagcagtgg ccgcgtgaaa ttctcaataa ttgctattag tgcacttcca tagtctttat
      961 gttcggtatg ctccaatatt gaaagcaccc tttcatcagt ggcggcctcc atatcctgcc
     1021 gaatgcattt aaaacaatac cacattatcg gattgaacca atgtactacc tgaataccaa
     1081 gcagcagtaa gtttatcaat atatcctttc gcttatagtg tgccagttcg tgtagtaata
     1141 catattcaat ttccttgtcg ctaagttttg ctgtttccgg cggcaatatt attttaggtt
     1201 ttataactcc gaataaagaa ggtatattga cagtattgct taccgtaaga catagatttt
     1261 tctttactcc tatccgtttt ttacattttt caaatacggt tattaccctt tgatctatat
     1321 cataggtttc tttttgaagt tttttataga aggacaaata ggtatacgtc agccataaca
     1381 gcataaacaa tgcaccaaaa aaccaaataa acgcaatagt cttttttgca ttggacttta
     1441 taatatctgt attaccgact gtgttattat taaaattctg ttgtgttggt atttcataat
     1501 tctcagtatt acttatttcc ttttctgtgg gcaatagaat ggttctttca ttataaatct
     1561 cagctgagtc gtcaatgctg ccaatttcat aggtcacagg tataatatta aataagctta
     1621 cagcactttc gggaccgaag ggtatgagca gtttcaaaat tagcacgatc cagataaggt
     1681 aatgccactt tgggcttaac ctgtttttta acaggttctt tattaaaatc agcacaatac
     1741 ccacaacagt accataaaga gaagtcatca atacaacact aaaaatgctt tccatctcta
     1801 ttcctcctca tccaacaact gcttcaggaa ttccagatct tttttggaaa gcttttgatc
     1861 tttaacaaaa cttgcaagca taaggttaac agaaccatta tatagttttt gtaaaaagga
     1921 tttattggca tagcttttat attgttcctc agaaacaatc ggataataga taaaagactt
     1981 gctgtttatt ttttcagcac ccactgcccc ttttgtaaca aggcgtgtta ttaaagtttg
     2041 aattgttttt ggcttccatt cggtagtttc ggtaagtttt tcgataatct gagagctggt
     2101 ggcctttcca aactcccaaa ggatcttcat aacttccaat tccgattctg tgatttgaga
     2161 ctctgctttc atatttaccc ctccaaatct ggattacaca aatctggatt acaaatgtaa
     2221 gttcaattat atcttacacc tgtaattcaa aattgtcaag gtttttgtta cacttaggat
     2281 aaattttatt cttttttctc tattaaaata taacttaacg ggatttgggc attcaattcc
     2341 tcttgttttc tatgtactgc agaacaatct ttgtcatgtt ctgaaaaata ttctatttat
     2401 tttgatttta tcacaaagca aacctgatta cataaataca tataatttta ttcactaatg
     2461 aagaattgca tcaaaactta taaaacacgt tgataaattt gataaaataa caaaaaaagc
     2521 tgcaattata gactggaata ctttgcaaac tttttataaa aataaatatc ttcaatattg
     2581 aaagatactt cttgtctgta atcttattct tatcatacct tatatccata ttttatcact
     2641 attcattttc tcccatccat taattttcct tctattgcat tatctctaga gattggcata
     2701 ttttcaagta caaaatagac cacccgcttt atttaagtat taatttcttg cagataagtt
     2761 aaattacata gaagtaaatt taataatttc aattattttg tcaacattag aacataaaac
     2821 acatttacct ataaattaat aaaataatat gcgtacaaaa ggattaaact atatatgaac
     2881 aaaatgcttc tttgttttta cttcatcgct tttgttcaaa gcttgctcgc aaaaagctca
     2941 aaacagcatt ttaagagcat tagtcccaat cgcttttgct cagattaatc cgtaaatggt
     3001 tgaaacaaca ataaactgga ggttcaatta tgggattctt tgatttttta tttaataacg
     3061 gcagcggaat taagacggat aacaacattg gacctactca aagcagacca ctggattttc
     3121 agccatataa tgaagagttt tctatggata tgacaacaga accttacgga aataacatgt
     3181 ttgaaagtaa taacactttt tcaaataatg gcgttgagct tcaagatgca aggaacacac
     3241 tcaggctcgt gtacagcggt attctcgcaa aaaatgacgc agatgaagtg tatgcggtag
     3301 taggctacgg aaacaatctt gcctgggaag ataccgaatc ttatcccatg cacaaaataa
     3361 gccacgataa attcgagctt atgcttcctg tcaaaaggcc cggtaacatc aacatcgctt
     3421 ttaaggatgg tacagagaac tgggataaca attccggtat gaattacagt tttaaaaact
     3481 atgtttacga gggtagccaa taagctaccc tcctctccaa taacccaaca caataaaact
     3541 tttctaaatt ttatgtatta agcctcttta gattcttttt ctacgctgcc aatcgcagat
     3601 tttacaaact tgatctttgt tttttcaaca ctggtctcaa ttgttatctc atcatcctta
     3661 atatttacta tctttcctat aataccactg gtggtaacaa cattatctcc cacttccagg
     3721 gcatcaagca tttgactgtg ctgcttatcc ctttttttct gaggaagaat cacaaataag
     3781 tacattgcac caatgaaacc gataggaatt atcagagtac ctattaaatt catatattcc
     3841 tgtggcataa caaacctcct tcttgtatta tgattttatt atatatttta ataattttaa
     3901 caactttatt tataaccgaa tttattaaag aactcatttc taaaatcacc gagcctgtct
     3961 tccattatag cttgtctgac attcttcatc agattaatta aaaaccttaa attatgccat
     4021 gtggtaagcc ttatcccgag gacttctccg gctttcaaaa gatgtcttaa atatgccctg
     4081 ctgaaatttc tgcaggtata gcagtcgcat tcggggtcaa ggcttgaata gtctctagca
     4141 taagcagcat cccttataat tacccttccg tttccggtca ttaccgttcc gttcctgcct
     4201 atccgggtgg gaagcacaca gtcgaacatg tctattcccc ttattgctcc ctcaatcagg
     4261 tagtcaggac ttcccacacc catcagatac cttggcctgt cctccggaag cagcggtacg
     4321 gtatgctcga gcatagcata catttcttcc gccggttctc ccacactcaa tcctccaatc
     4381 gcgtatccgg gaaaatccaa ttccagcaat tcataagcac tctgctctct gaggtcctta
     4441 tacataccac cctgcacaat accgaaaaga gcttgtgtat cgggatttcg gtgagtttcc
     4501 ttacatcttt tagcccatct tgtggttctt tccaaggatt tttttgcata atcatactcg
     4561 gctggatacg gaatacactc atcaaaggcc attataatat cggcacccaa atcattttgt
     4621 atcctggtag ccatctctgg agttataaaa tgcttggatc cgtcaatatg agacttgaaa
     4681 gtcacgcctt cttcctttat atccctcaga tcgctaaggc tgaaaacctg aaaccctccg
     4741 ctatcggtga gaatcggcct gtcccagttc ataaagccgt gaaggcctcc tgcctccttg
     4801 actatatcct ctcccggtct catatggagg tgatatgtat tactcaatat tatctgggct
     4861 tcaatatcct taagctcatc cggagacatt cccttaacgg ttgcctgcgt ccccaccggc
     4921 ataaacgccg gagtatcaaa actcccgtgg ggagtatgaa cccgtcccaa tcttgctcct
     4981 gtttgtttac actttttaat caactcatat ctaattgcac ccatgctcta tccgattcct
     5041 taatatattt ttataggttt cctgaaaaac ctttacatta atgaaatatc aaaaagttaa
     5101 aaacactcta aataactgca attttattta ttcattttat aaacatggca tctccaaagc
     5161 tgaagaacct gtatctctcc cttactgccg tattatatgc ttccattatc ctgtcccgtc
     5221 ctgcaaaagc actgaccagc attataagtg tggattcagg caaatgaaaa ttcgtaataa
     5281 gggcatccac aactttaaac ctgtatccgg gataaataaa tatatcggtc catccactct
     5341 caggattcat ccttccgctg tcatcgccca ccgtctcaag cacacggcag cttgtggtac
     5401 ccacagcaat tacccgtttt cccttttctt tggccgaatt tatcttccgg caatcctctt
     5461 cgtttatcct gtagtactcg ctatgcattt tatgttccag tacgtcttca acctttacag
     5521 gccggaaagt acccagacct acatgcagcg taacgtaagc aatgtccacg cctttatttc
     5581 ttatctcatc caacagctcc tttgtaaaat gaagcccggc agtaggagct gcagcagagc
     5641 ccttatattt tgaatatacg gtctgatacc tctcagcatc atccagcttt tttgtaatgt
     5701 aaggcggcag cggaacaata ccgaccctgt ccaaaacctc ttcaaagatg ccgtcatact
     5761 cgaatcttac aagcctgttg ccttcttcta caatttctac aacctcggct ttaagaacac
     5821 cgtctccaaa cacaaacttt gaacccggtt tagccctttt tcccggtttt aaaatgactt
     5881 cccatatatc attctcaatc tttttgagca aaacaaactc gattttgccg ccggttccct
     5941 ctttttctcc aaggagtctt gccggaatta cccttgtatc attcaaaacc aggcagtcgc
     6001 cttcgttgac atactctaaa acgtctttaa atatcctgtg ttcaatactg cccgtattct
     6061 tatccagcac caaaagcctt gacatttgcc ggttcggaat cggttcttgg gcaataagtt
     6121 cctggggtaa atcataatta aaatcgctga ccttcataat taaaacaaca ccgtctcata
     6181 ctatatttta agcaaaactt gttttaaata accatatgat agaataatac attaaatcaa
     6241 ttcatcctgc aaggctttat ttgattaagg atacatattt tactcatatt tattcaatac
     6301 atcgcttttt ggtcatttga cttcctgaaa tcccactaat aaaaaagagc caatcataat
     6361 tgactgaccc tccattaatt gttttataaa cagtatgaag ctgtgtacta caattatata
     6421 caaaatttaa tttactcgac ctttgtcccc gcaaaataat gagccaatat ttctttatag
     6481 gtaaagcccg ctttagccat tcctttagca ccctcctggc tcataccgac agaatggccc
     6541 cagccctttc ctataatagt ataaatcgtt ggaattatag ggattttctt gatgctgccg
     6601 gaacttacca cgcttacact gtttcccgat gcacttatgg tttttaaacc gctagctgtc
     6661 attaccttct ttcctcccag ctgtgtcttc accggttctc cttcaactcc cataacaaga
     6721 gtatccgcat cggtagtaat ttcgtaccat tgactgtcaa ggcttaaaaa gcttcttgtt
     6781 gagccattcg tatatattct ctcatccttg ctgcctttaa ctaccacctc agtggctctt
     6841 cctgcctcgg atcttttcgt tatgaaaatt cccagaatat ctccgagatt aaaccccctg
     6901 tcaatcatta tttgctttat tttttgtgcg gtaaaggata cctcccaatt gtaatgccat
     6961 gagtcaccgg attcatattt gtcctcaaca cttattagat acgggtaacc gtcactaccc
     7021 catacattct ttacatcctc cgtcctgcct ccgcttgagc tgaaatagaa caccgatgca
     7081 ggctttccat tgtatgtaa
//
LOCUS       DAOOJC010000070         7046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_8904_length_7046_cov_3.301817, whole genome shotgun sequence.
ACCESSION   DAOOJC010000070 DAOOJC010000000
VERSION     DAOOJC010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8904_length_7046_cov_3.301817"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLA01_03150"
     CDS             <1..231
                     /locus_tag="PLA01_03150"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPD00329.1"
                     /translation="PEEMYEIGFEEYLYGDGLVVIEWADLIDDILPRENIWITIKKDL
                     TQGMDARVIQIEFSGDRYYEYEKKLTAGDGNK"
     gene            231..944
                     /gene="tsaB"
                     /locus_tag="PLA01_03155"
     CDS             231..944
                     /gene="tsaB"
                     /locus_tag="PLA01_03155"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPD00330.1"
                     /translation="MKILALDSSAMVAAVAVMEDEKLLGEYMLNHKKTHSQTLMPMVK
                     QILGDLEMAPEDIDVFAASVGPGSFTGLRIGVTTVKAMAYALRKPVVSVPTLDAIAYN
                     IPMSSYTVCPMIDARNNQVYTALYDWKENSLERITEYMGIPVAQLVKIIKDADKKVVF
                     AGDAVQLHEEFLKNELGDGCRLAPGSLRLQRASSVAHIAYIKASKGELENSFDMVPFY
                     LRKSQAEREYEKKLCNEKK"
     gene            1115..3652
                     /locus_tag="PLA01_03160"
     CDS             1115..3652
                     /locus_tag="PLA01_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPD00331.1"
                     /translation="MRKRGLGFLVSIAVIITLFGCFLDLQGNAAANEYKFDFGGGAVE
                     WGYIGVSASTAYSKSQGYGFNTPWNMKNVTASGSGLTSDAVQFLVYGTKSENTFNVDL
                     DNGLYEVKVTLGNTQRASVAAEGVYQIINMTGNCATDKFQIPITDGQLNILVTEGKAG
                     TAFTLSALEIKKISDVPVTNRTIYIGGDSTVCNYYPLDSSVQAGWGQMLSKFVDTNTF
                     QIRNMASSGQIARGFRDDGQFEAIMKYLKTGDLFILQFGINDTNEKNSTTEAQFKEIM
                     TDMVVKAKATGATVVLSTPQGRATDFNSSNVHNSQGRWYRNATIQVAGEQGVRLVDLN
                     VLSSQYFTSIGPNATSVLYMSGDSLHPNREGATQLARIVAEELSDLLKDTPVVTPART
                     PVVTPAKTPVPTLNDYLYGDVDGSGTVDSTDCTLLKRYLLRKVKSFPYENGTIVADVN
                     ADGSIDSTDLTLLKRFILRVIKEFPADKQKNETVIYQAEDAIISKALLETVNGGYTGD
                     SYVNYYNEVGGYIEWNVNAPSSGPYALTFRFANGTAANRPTEIRVNGNIIKSGMDFNS
                     TGSWTDWNETSVVVELNKGNNVIRATAIASDGGPNVDYLKVSPSTDPVETPEPAVTPG
                     PTSNEKISIYIASDSTAQTYISSYAPQAGWGQFLGQYFTSNVVITNKAIGGRSSKSFV
                     VEGRLDEILNVIKPGDYLFIQFGHNDATISNPDRYAAPYTTYKEYLGKFVDGARQKGA
                     IPVLITPVARLNYKNNSFVNDFPDYCTAMKQVAQEKNVKLIDLMSKSLNYYTSIGYNE
                     VFTYYMVSVNNTDYTHFTEKGAKQIARLVAEGVKETNLDITKYLNAN"
     gene            4004..4459
                     /gene="rimI"
                     /locus_tag="PLA01_03165"
     CDS             4004..4459
                     /gene="rimI"
                     /locus_tag="PLA01_03165"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPD00332.1"
                     /translation="MTFDGIEISYMGIEDIDDVMVVEKLSFKIPWSRNALTEEVTNNK
                     FAKYIVAKVNGRVVGYAGMWKVCDEGHITNIAVHPEYRRQGIGNKLVENLILIAEEEG
                     IIRMTLEVRRSNSAAQNMYKKHGFVEEGFRKAYYADNGEDAIIMWKENK"
     gene            4742..>7046
                     /locus_tag="PLA01_03170"
     CDS             4742..>7046
                     /locus_tag="PLA01_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD00333.1"
                     /translation="MSQIKGLSANMLRKECDPSSLTFANTSELEPLEDIIGQERAVRS
                     MEFGLKIDTRGYNIFMSGMTGTGKTSYAVNCAKKIAKKSKAPDDWCYIYNFENPNQPK
                     AINLPAGLGKVFQKDMEDFIKVLQLEISRAFDSEDYEREKTAIAKEFQEKRAELLDEL
                     NEDAEKQGFKVKTTNAGIYFLPVIEGRTITEEEYGELDEKIKQEITEKSNIVQLETID
                     IIRKIKNVEKEAEERVTEWENKIALFAVGMQINDMKEKYKDYKKIISYLENVQEDILK
                     NLGDFREEELTEEQQIIIPWLRGNDASPTDKYKVNLLVDNSALEGAPVIVDFNPTYYN
                     LLGKLEYENEFGTMTTDYTMIKAGLFHQANGGYLILQAKDVLSNVQSWEALKRVLKTR
                     QITIENMKEQMGLVAVSALKPEPIPLKVKVILVGSEYLHQALYEIDEDFKKLFKIKVD
                     FDAEMERNEENTEKLAQFISSFCRRENAPHFNRTGVAKVVEYCSRLAEDQIKLSTKFN
                     EIVEILCESSAWAQIEGSDEVRAAHVRKAIKEKIYRSNKYDKKLLELISDGTIIIDTE
                     GEAVGEINGLSILDMGDYCFGKPSKITANTFMGGKGIVNIEREVEMSGTTHTKGVLIL
                     SGYIGEKYAQDMPLSLTASLCFEQLYNGIDGDSASSAELYAILSSLAEVPIKQSIAVT
                     GSVNQKGEIQPIGGVNEKIEGYFELCKLRGLDGTHGVIIPYRNIRNLNLNDDVVEAVE
                     KGKFNVYAVKTIDEGLEILTGIKFGDKG"
BASE COUNT         2379 a         1120 c         1679 g         1868 t
ORIGIN      
        1 cctgaggaaa tgtacgaaat cgggtttgag gaatacttat acggtgatgg actagtagtg
       61 attgagtggg cggatttgat tgatgatatt cttccccgtg aaaatatatg gatcacaata
      121 aaaaaggacc tcacacaggg aatggatgcc cgggtcattc aaattgagtt tagcggtgat
      181 agatattatg aatatgaaaa aaagctaacg gcaggagatg gaaataaata atgaaaatat
      241 tggcactgga ttcgtcggca atggtggcgg ctgttgctgt aatggaagac gagaaacttt
      301 tgggagagta tatgttgaat cataaaaaaa cccattccca aacccttatg cccatggtga
      361 agcaaatcct tggagatctt gagatggctc cggaggatat agatgttttt gcagcatccg
      421 tcgggccggg ctcatttacg ggacttcgga tcggagtaac taccgtaaag gctatggcgt
      481 atgcactaag aaagccggtt gtcagtgtgc ctacattgga tgccatagca tataatattc
      541 cgatgagcag ctataccgta tgtcctatga tagatgcaag aaacaatcag gtgtataccg
      601 ctctttatga ctggaaggag aactcattgg agagaataac cgaatacatg ggaataccgg
      661 tagcacagtt agtcaaaatt ataaaggatg cggataaaaa agttgttttt gccggtgatg
      721 ccgtgcagtt acatgaagaa ttcctgaaaa atgagctggg agacggatgc cggcttgctc
      781 ccgggagttt gaggctccaa agggcatcct ctgttgcaca tattgcttat ataaaggcat
      841 ccaagggaga acttgaaaac agttttgata tggtcccctt ctacctaaga aaatcccaag
      901 ccgaaagaga atatgaaaaa aagctgtgca atgaaaaaaa gtaaaataga ataataccct
      961 taaaatattc ggcttctgcg tatatatcta taaatcacat aaatagttgt ttttttaggg
     1021 gaatatcatt tatgaataat aaagatattt ctttgatgct ggtatctaat ttttagttta
     1081 atttatataa ggttttaaaa caggagggga tattatgcgg aaaagaggat tgggattttt
     1141 agtcagcatt gctgtaataa taaccttgtt tgggtgtttt ttagatttgc aaggtaatgc
     1201 ggctgcaaat gagtacaaat ttgatttcgg tgggggtgct gtagaatggg gttatatagg
     1261 tgttagtgct tccacagctt atagtaaatc ccaaggctat ggttttaata ctccatggaa
     1321 tatgaagaat gttacggcat cgggcagtgg ccttacaagt gatgctgtcc aattcttggt
     1381 atatggcaca aaaagcgaaa atacttttaa tgtcgatctt gacaatgggc tgtatgaggt
     1441 caaggtcact ttgggaaaca ctcaaagagc aagtgtggca gcagagggtg tttatcaaat
     1501 aataaacatg accggaaatt gtgcaacgga caagtttcaa atccctatta ccgacggaca
     1561 attaaacata ctggtaacag agggaaaagc aggtactgca tttaccctca gtgcccttga
     1621 gataaaaaag atatccgatg ttcctgtaac aaacaggaca atttatatag ggggagattc
     1681 gacagtctgc aactattatc cgctggactc aagtgttcag gcaggttggg ggcagatgct
     1741 atcgaaattt gtcgatacca acacttttca aataagaaat atggcatcat cgggtcagat
     1801 tgcaagaggt tttcgggacg acggacaatt tgaggcaata atgaaatacc ttaagaccgg
     1861 tgacttattc atattacaat tcggtataaa tgacactaat gaaaaaaact caacaacgga
     1921 agcacaattt aaagaaatta tgaccgatat ggttgtaaag gcaaaggcta ccggtgccac
     1981 tgtagtattg tccactcctc aggggagggc caccgacttt aattcatcca atgtacataa
     2041 ttctcaaggc agatggtata gaaatgctac tatacaagtg gccggagaac aaggtgttcg
     2101 tttggtcgac cttaatgtct taagctcaca atatttcacg tcaattggcc cgaatgcgac
     2161 aagtgtactg tatatgtccg gggacagctt gcatcctaac cgtgaggggg ctacccagct
     2221 tgcccgtatt gtagcagagg aattgtcaga ccttctgaaa gacacacctg ttgttacccc
     2281 tgcaagaaca cctgttgtga ctcctgcaaa aaccccggta ccgactttaa acgactatct
     2341 ttatggtgat gttgatggca gtgggactgt agattccacc gactgtacat tgctaaaaag
     2401 atatttattg agaaaagtta aatctttccc ctatgaaaac ggtactatag tggcagatgt
     2461 aaatgcagac ggttcaatag actcaactga cttaactttg cttaaaaggt ttatattaag
     2521 ggtaattaaa gaatttccgg cagataagca aaaaaatgaa acagtaatat accaggctga
     2581 agatgccata atatccaagg ctttattgga aaccgttaac ggagggtata cgggagactc
     2641 atatgtgaat tattacaatg aagtcggcgg gtatattgag tggaatgtta atgcaccttc
     2701 ttcaggcccg tatgcactta cattcaggtt tgcaaacgga acagctgcca acaggccaac
     2761 ggaaataaga gttaacggaa atattattaa gtccggtatg gattttaatt caacagggtc
     2821 atggactgat tggaatgaaa caagtgtagt ggtagagctc aacaaaggaa ataacgttat
     2881 cagggcgaca gctattgcat ccgatggagg acccaatgtt gactatttaa aggtatcccc
     2941 ctcaactgat ccggttgaaa caccagaacc tgctgtgaca ccgggtccta cgtccaatga
     3001 aaaaatatca atttacattg ccagcgattc cactgctcag acatatatct cgtcttatgc
     3061 acctcaagcc ggatggggac agtttttggg tcaatatttt acttcaaatg ttgtaattac
     3121 caataaggca attggaggaa gaagttctaa gagttttgta gttgaaggca gattggacga
     3181 gattttaaat gtaataaagc ccggtgacta tttatttatt caatttggac ataatgatgc
     3241 aactataagc aatcccgacc gctatgccgc tccatatacg acatataagg agtatcttgg
     3301 caaattcgta gacggagcac gtcagaaagg tgcaataccg gtattgataa caccggtggc
     3361 aaggcttaat tacaaaaaca acagctttgt caatgatttt ccggattatt gcacagccat
     3421 gaagcaagta gcccaggaaa agaatgtcaa gctgatagac ctgatgtcaa agagcttaaa
     3481 ttactatact tcgataggct acaatgaagt atttacatac tatatggtat ctgtgaacaa
     3541 tacggattat acgcacttta cagaaaaagg agctaagcag attgcccggt tagtggcaga
     3601 aggtgttaaa gaaacaaatt tggacattac aaagtattta aatgccaact aacggataaa
     3661 gactatgacg gagatatccg attacaaaat atggttgaca taaacatttt attgcgatgt
     3721 gaatattaaa ttaaccatac cctttgagtt agtatttgtt gtagcagcaa cagatacttt
     3781 tttttagtgg gttttcacga agtgaaatga ccgaaaagcg attgtttaaa acactgtaat
     3841 tcagggctaa aatatggtaa gataattgac gtaaatagag tgcgattcaa attacattta
     3901 aagaaattat tttgtagaga taattttgcg gtattagcag taaagggttt acttgcatat
     3961 aatattctaa aaacctccac aaaggataaa aaggtgagaa ttgatgactt ttgacggtat
     4021 tgaaatatcc tatatgggaa ttgaagacat cgatgatgtt atggttgtgg aaaaattgag
     4081 ctttaaaatt ccgtggtcga gaaatgccct gactgaggaa gttacaaaca acaagtttgc
     4141 aaaatatatt gttgcaaagg tgaacggaag ggttgtgggt tacgccggga tgtggaaggt
     4201 ttgcgatgaa gggcatatta caaatattgc ggtccacccg gaataccgaa gacagggcat
     4261 aggcaataaa ctggttgaga acctgatact tatagccgaa gaagaaggca taataagaat
     4321 gaccttagaa gtaaggagga gcaattccgc tgcacagaat atgtacaaaa aacatggctt
     4381 tgtggaggaa gggttcagaa aggcttatta tgcggataat ggtgaagatg caataataat
     4441 gtggaaagaa aataaataga aaaattttag aggattttaa catatcggag tagaatataa
     4501 tatgttaaga aaacagccat aaatgtctgt aacggtcatg tgaaaaatca atgaaatgcg
     4561 atggattttt gttatatgac agtattaagt gtttcgagtt caaattccga aataaatatt
     4621 aattgtgaaa ttgaaatctg accaaaagca agagaatttt taatgtcgat gagtcttgtg
     4681 ccgagtattg tgacactatc gtctaaggac ttcaaatagc cggataaaag ggggaaagtg
     4741 tatgtcacaa atcaaaggtt tgtctgctaa tatgcttaga aaggaatgtg acccgagttc
     4801 tttaactttt gccaatactt cggagttgga accccttgag gacattattg gtcaggagcg
     4861 tgctgttcgt tcgatggaat ttggacttaa aatagatacg cggggctata atatttttat
     4921 gagcggtatg accggtacgg gaaaaaccag ttatgccgtg aactgtgcga aaaaaattgc
     4981 aaagaaatca aaggcacctg atgactggtg ctatatatat aattttgaaa atccaaacca
     5041 gcccaaagca ataaaccttc cggccggttt aggcaaggta tttcaaaagg atatggagga
     5101 tttcataaag gttttacagc tcgaaatatc ccgggctttt gacagtgagg attatgaaag
     5161 ggagaagact gcaatagcaa aagagttcca ggagaaaagg gcggaacttt tggatgagtt
     5221 gaacgaagat gcagaaaagc agggattcaa agtaaaaacg acaaatgcag gtatatactt
     5281 tttaccagtg attgaaggca ggacaataac tgaggaagag tatggggaat tagatgagaa
     5341 gattaagcag gagataacgg aaaagtcaaa cattgttcag cttgagacca ttgatataat
     5401 aagaaagatt aagaatgtgg agaaggaagc ggaagaaagg gttaccgaat gggaaaataa
     5461 aattgcattg ttcgctgttg gcatgcagat aaatgatatg aaagaaaaat acaaggatta
     5521 taagaaaatt atcagctacc tggaaaatgt tcaggaggat attctcaaaa atctcggtga
     5581 tttcagggaa gaagaactga cggaggaaca gcagataata ataccatggt taagaggtaa
     5641 tgatgcatcg ccaacggata aatacaaagt taatttactg gtggataact cagccctcga
     5701 aggagctccc gtcatcgtgg atttcaatcc tacctattat aatcttcttg gcaagctgga
     5761 atatgaaaat gagttcggaa ccatgaccac cgattataca atgattaaag ccggactgtt
     5821 tcatcaggct aatgggggtt acctgatact tcaggcaaag gatgttttaa gtaatgttca
     5881 gtcctgggaa gcacttaaaa gggttctaaa aacccggcag attacaatag agaatatgaa
     5941 ggagcaaatg ggattagttg ctgtttctgc attaaagccg gagcccattc cgctgaaggt
     6001 caaggtgatt ttagtgggaa gcgagtacct gcatcaggct ctttatgaaa ttgatgagga
     6061 tttcaaaaag ctgtttaaga ttaaggtaga ctttgatgca gagatggaga gaaatgaaga
     6121 aaacacagaa aaacttgctc agtttataag ctccttttgt cgcagagaaa acgcacctca
     6181 ttttaacaga acaggggttg ccaaagttgt ggagtactgt tcacggcttg ctgaggacca
     6241 aatcaagctg tctaccaagt tcaacgaaat tgtcgagata ttgtgcgagt cgtcggcatg
     6301 ggcccagatt gaaggaagcg atgaggtaag ggcggcacat gtgagaaaag cgatcaagga
     6361 gaagatttat agatccaata agtatgacaa gaagcttttg gagttgataa gtgacggaac
     6421 aataatcata gatactgaag gagaggctgt tggagaaata aacggtctta gtatactcga
     6481 tatgggtgat tactgttttg gaaaaccttc aaagataacc gcaaacacct ttatgggtgg
     6541 aaagggaatt gtaaacatcg aaagagaagt tgaaatgagc ggaaccactc atacaaaagg
     6601 ggttctgata ttgagcggat atataggtga aaaatacgct caggatatgc cgctatccct
     6661 tacggcaagc ttatgctttg aacagctgta taacgggatt gatggtgaca gtgcatcaag
     6721 tgcagaactt tatgctatct tatcaagcct tgcggaagta cctataaaac aaagtattgc
     6781 agtaaccggt tctgttaacc aaaagggaga gatacagccg ataggtggag ttaatgaaaa
     6841 aattgagggg tatttcgaac tgtgtaagct gcgtggactt gacggtactc atggagtgat
     6901 aattccgtat cggaacataa gaaatctcaa tctgaacgac gatgttgttg aagcggttga
     6961 gaaaggtaaa ttcaatgttt atgcagtaaa gacaatagat gaaggtttgg aaatactgac
     7021 aggaattaag ttcggcgata aagggg
//
LOCUS       DAOOJC010000071         6994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_8969_length_6994_cov_4.522554, whole genome shotgun sequence.
ACCESSION   DAOOJC010000071 DAOOJC010000000
VERSION     DAOOJC010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8969_length_6994_cov_4.522554"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1089..1616)
                     /locus_tag="PLA01_03175"
     CDS             complement(1089..1616)
                     /locus_tag="PLA01_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPD00334.1"
                     /translation="MDFIKDVTKKVASTAKVAVKKSGVLVEITKLNLNIGTEKDKISK
                     IYTQIGKAVCDSFEKGEDVPSNYTVLCEKVLNIKENIEKMKRQILQLKNIKICPSCGA
                     ELEVDVAYCSRCGAKQETPACSELVADAQDCRDCHNDDVESGDDEADKIADEDSEVNN
                     AAEEDEANSPAEEEE"
     gene            complement(1780..3054)
                     /locus_tag="PLA01_03180"
     CDS             complement(1780..3054)
                     /locus_tag="PLA01_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00335.1"
                     /translation="MNNKFKYLRSFLIFDQEDSRFKEGGDSSGYVKIEVRDGKGKLSC
                     QVSNLKEDTKTNYKLYMIKADDSDLTAVCAGSIPLAKGRGELNWSFNPSNVDCSGLTI
                     EDFNIMAVLAENDDGHFGNVLCPLVSYKGSKIEWRHKMKRFLSDRQVTKEESAEKPDN
                     KKASDVDKNIEKENKDIPAEELISKYENVIESKYVKTSAGDGQREDDNEYGGNKEKDA
                     DTGITVQDNEQSKIFEPEIENNEEKNKEGSTGEHSDKNEQEQSMEERNTEGQNRISGN
                     AVQNKNESEAEGHRPGVRELAESFDRYFEKYNPFKGGRKEYRWWKVSSPVHLNNILYQ
                     MDIKVPILFNPMVLMAHFKHRHLIVGTYEDDARDLSYIVCGVPGVYWVDEKPFGNICR
                     WAQVEGSVPKYGAFGYWLVYINPKNGEILNIS"
     gene            complement(3288..4130)
                     /gene="rsmA"
                     /locus_tag="PLA01_03185"
     CDS             complement(3288..4130)
                     /gene="rsmA"
                     /locus_tag="PLA01_03185"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPD00336.1"
                     /translation="MAESTRNLIQKHNIKLTKSLGQNFLTDERIVKKIVDTAELTDGD
                     TVIEIGPGVGSMTVELAKRAEKVIAVEIDKRLIPALKENLDGFENVHIINMDIMDVDV
                     KELQTGAQTRKLKVVANLPYYITTPIIMKLLEEDDNISLMVFMVQKEVAHRMAAQPGG
                     KDYGSLSVAVQYYSKPEIAFDVSPHCFVPQPDVDSTVIRLKRNETPPVRLLDKELFFR
                     TVKASFGQRRKTLVNALYNCGGFQKTKEEIKEILHSLGIDENVRGEVLSITQFAELSN
                     AFYK"
     gene            complement(4290..5342)
                     /locus_tag="PLA01_03190"
     CDS             complement(4290..5342)
                     /locus_tag="PLA01_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HPD00337.1"
                     /translation="MLSLVKEIKRCISLKMVAVVITAAVIALMVGTVSYFQKEVVINL
                     DGNRMVVRTVKSTVKEVLEQNGIRLGKYDYVSVPIDTKLQSKENNIINIRSAVPVYVF
                     ADGREIELMTSKETVKEALRDEPINLSHSDRIEGAEPDDKIVKDMRLKVVRVTRKLVS
                     QNEIIPCEVVNKDNNSMDMGQYRVINEGKDGVREKVYMLEYEDGKEVKRLLVDHKVVV
                     NPVDRLVEYGTIPIYTTSRGENFRYKKVMEMRATAYTASYEDTGKTPDHPHFGITYTG
                     MRVKKGVVAVDPKVIPLGTKLYIEGIGNVPDYGYAIAADIGSAVKGDIIDLYMDDKGA
                     VDKWGVKKVKVYILYD"
     gene            complement(5688..6122)
                     /locus_tag="PLA01_03195"
     CDS             complement(5688..6122)
                     /locus_tag="PLA01_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00338.1"
                     /translation="MEKKFNKEPEEISPDMTGVNRMEQIPQIPQIPQMPQMPEAQPMP
                     QMGAMPMPEQIPQLGPMPIMQQPQVQPPQMQQPQMVPQIVCCPLLISMQCPFMQHEYA
                     AMGQGMAPSPYMSNCQGADQYKMHQMASPYPCNPNMCGRYMY"
     gene            complement(6213..6980)
                     /locus_tag="PLA01_03200"
     CDS             complement(6213..6980)
                     /locus_tag="PLA01_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPD00339.1"
                     /translation="MFFDSHAHYDDERFENDRLETIMKAFESGVAYILNASSNITSSM
                     DTVSLTQKFDFVYGAVGVHPHCASEISDNTYVALRDFTKDDKIVAIGEIGLDYYYEYS
                     PRDLQKLHFAKQIALAKELDLPIIIHDRDAHKDTMDIVKSEQAKDVGGVFHCYSGSVE
                     MLREVLDNNFYISVGGALTFKNAKKLVEVVEYVPLDRLMIETDCPYLTPEPHRGKRND
                     SGYVSLVAQKISAIRGIELEKVAELTTDNAKRLFRIQ"
BASE COUNT         1994 a         1632 c         1030 g         2338 t
ORIGIN      
        1 acaactgcaa ttagaattga aattcttctt ttaaccattc tctccctctt ctcctttcaa
       61 atatcaattt tattcttttt aagttatatg atgattaata atgcaattta cccacaattc
      121 atccattccc cctttttgcg gatacatttt atgagcatta tttacatcat aaaactaagc
      181 tggacttaaa aaaaactttt aatttatatt aaaatttaat tgttatccct gtaactaaaa
      241 agccattatc agcctctatt tattcaataa aaaacaacaa taaacgtata cttatatcaa
      301 ataagctatt tgtagtatac aaaatcaata cctactgcta aaaaagccat gcatttgaca
      361 agaattgaaa agacaatccg taaatcaatt caccaaggct taaatgctat aacgataata
      421 atgaaaggtt taagaacact agctatcaga gaacaataat attgattgcg aaaaagtgtt
      481 tacgcatgct caatcactac ttcaataata ctacttttct tgtgggaatt ttaaaatagg
      541 ctggtaaaat cctaacaaca aatgcacaca agcaaattaa gcagtccaaa tattcagttt
      601 tcaatgaacc aaaccatcaa taactttacc taatatataa atttcgcata tatttatttg
      661 ttttgggggg taaaaagaaa tcgattaaaa aacatttttt tgacaaaaat actaaaaaag
      721 cttcattttt tagtataaga cttgagaaat atttaatatg taatgcaaaa aaccctctca
      781 atacttcccc caccaacaaa atcctttgac ggtttacagt aattctaagt ttataacaat
      841 actaaaccgt atcttaatta taatacacat cgtctttcta tgcaagtaat aaaattgtgt
      901 ttgattaaaa aaccttgctg aataagagct caaaaacctc aaatttcttg cttttcagca
      961 cctatacttt tatgaaattc cgctgaaata aaaatgtcta tgtgccctgc caaagcccat
     1021 agactgtcat attctatcaa ggtatattta cataaactta cttatgtctg caatataaaa
     1081 tcaaacaatt attcttcctc ttctgccggc gaattcgctt cgtcttcttc tgcagcatta
     1141 tttacttcac tgtcttcgtc cgcaattttg tccgcttcat catcaccgga ttccacatca
     1201 tcgttatggc aatcccggca gtcctgtgca tctgcaacaa gttcgctgca tgccggtgtt
     1261 tcctgttttg caccgcatct tgaacaatag gcaacgtcaa cctcaagctc tgccccacat
     1321 gacggacata tcttgatatt tttcaattga agaatctgcc gcttcatttt ctcaatgttc
     1381 tccttaatat ttaaaacctt ttcacagaga actgtataat tcgaaggcac atcttctccc
     1441 ttttcaaagg aatcacatac tgctttccct atttgtgtat agatttttga aatcttgtct
     1501 ttttccgttc cgatatttaa attgagcttt gtaatttcta ctaaaacacc ggattttttc
     1561 acagcaactt tagccgtact ggcaactttc tttgtaacat cctttataaa atccattgct
     1621 tatctccccc tcacccgtat gattaattac actatatata atacactaat ttttaaaaga
     1681 tgccaatagt ttttataatt tttttaatta attaattaat attattaatg tgttggttaa
     1741 taaaaagaaa aaacgtataa acaattcatg caatattgat tagcttatgt ttaaaatttc
     1801 accgttcttc gggtttatgt aaaccaacca atagccaaaa gcaccgtatt tggggacgct
     1861 cccctcaacc tgtgcccatc tgcaaatatt cccaaagggt ttctcatcca cccaatacac
     1921 tcctggaacc ccgcatacta tatatgacaa gtcccgggca tcgtcttcat aagttcccac
     1981 aatcaaatgc ctgtgtttaa aatgtgccat cagcaccatc ggattaaaga gtatcggaac
     2041 ctttatatcc atttgataaa gtatattatt caaatggacc gggctggata ccttccacca
     2101 tctgtattcc tttctccctc ccttaaacgg attgtacttt tcaaagtatc tgtcaaagct
     2161 ctccgccaat tctctcacac ccggtctatg accttcggct tcggattcgt ttttattctg
     2221 taccgcgtta ccggaaatac ggttttgacc ctctgtattt ctctcttcca tgctttgctc
     2281 ctgctcgttc ttgtctgaat gttctccggt actgccttct ttattttttt cttcattgtt
     2341 ttctatttcc ggctcaaaaa tctttgattg ttcattatcc tgaactgtta tgccggtatc
     2401 ggcgtctttt tctttattac cgccgtattc attgtcatct tccctctgcc cgtccccagc
     2461 agaagttttt acatatttac tctctataac attctcatat ttgcttatca gttcttcagc
     2521 cggaatgtct ttgttctctt tttcaatatt cttgtctaca tctgaggctt tcttattatc
     2581 gggcttttct gcggattcct ctttggtaac ctgacggtca ctaaggaatc tcttcatttt
     2641 atgtctccac tcaatcttac tgcccttata tgaaactaat ggacataaca catttccaaa
     2701 atgcccatca tcattttcag ccaatacagc cataatgttg aaatcttcaa tagttaagcc
     2761 cgaacaatct acattggaag gattaaagct ccaattcaac tcgcccctgc ccttcgccaa
     2821 cgggattgag cccgcacaaa ccgctgtcaa atcggaatca tcagccttaa tcatgtacag
     2881 tttgtaattt gttttagtat cttccttcag attggatacc tgacaagaca gcttcccctt
     2941 tccatccctg acctcgattt ttacatatcc cgatgaatct ccgccttctt taaaccttga
     3001 atcctcctgg tcaaaaatca aaaatgacct gagatatttg aatttgttat tcatttttta
     3061 cccccacctt tataaaatta gtcttggccc gattatacaa ataaagttcc ataacccctt
     3121 ccgattaatt cctgaaaaat aatcatattt taaagtgcat acttatatat atgttgtttg
     3181 ataaaaaaat aaaccgttta aactttttga aagtaaaaaa cggcttacct tatatattaa
     3241 agatatttgt atttataaga tgcaattttc atttatgctt acaggaatca tttataaaaa
     3301 gcattcgaaa gctctgcaaa ctgtgtaatc gacaaaactt cccctctcac attctcgtca
     3361 atccccagac tgtgcagtat ttctttgatc tcctccttcg tcttctgaaa acctccgcag
     3421 ttgtacaaag cattcaccag cgttttcctc ctctgaccga aagaagcctt taccgtcctg
     3481 aaaaacagct ccttatccag aagccttacc ggaggtgttt catttctttt caacctgata
     3541 acggtagaat ccacatcagg ttgcggaaca aagcaatggg gagaaacatc aaaggctatc
     3601 tcaggctttg aataatactg cacggcaacc gataaagagc cataatcctt ccctcccggt
     3661 tgtgccgcca ttctatgggc tacctccttt tgcaccataa ataccataag gcttatgtta
     3721 tcgtcctcct ccaaaagctt cataataatc ggagtagtta tatagtaggg caggtttgca
     3781 accaccttta actttctggt ttgagctcct gtctgaagct ctttgacatc aacatccatt
     3841 atatccatat taataatgtg gacgttctca aacccatcaa ggttttcttt cagtgcagga
     3901 ataagcctct tgtcaatttc tacggctatt accttttctg cccgtttagc cagttcaacc
     3961 gtcatgctgc caacacccgg tccgatttca attaccgtat cgccgtcagt caattcagcc
     4021 gtatcaacta tttttttaac aatcctctca tcggtaagaa aattctgtcc cagggatttt
     4081 gtcagtttta tattgtgttt ttgaatcaga tttcttgtgc tttctgccat aattccaact
     4141 ccaaaaaatt ttttaaaaac atgatgtaag aaaatttctc tgctaaagac aattttcttt
     4201 gaattgaagc attataaaac agccgggacg attaatcccg gctcaattac agtcccttta
     4261 actattttag ttccaatatg tacagataat caatcgtaaa gaatataaac ctttaccttc
     4321 tttactcccc atttgtcaac cgcacccttg tcgtccatgt agaggtcaat aatatcgccc
     4381 tttaccgcac ttccgatatc tgctgcgatt gcatatccgt aatcgggaac atttcctatg
     4441 ccttcgatat acagcttggt tcccagagga ataacctttg gatctactgc aacaacacct
     4501 tttttcaccc gcatgcctgt ataggtaatt ccaaaatggg ggtgatccgg tgttttgccg
     4561 gtatcctcat aacttgccgt ataggcggtg gctctcattt ccataacctt tttatacctg
     4621 aaattctctc ctcttgaggt agtatagatg ggaatcgtac catactctac aagcctgtct
     4681 acgggattaa ccacaacctt gtgatccacc agcagccttt taacttcttt accgtcttca
     4741 tactctaaca tgtagacctt ctctcttact ccatccttac cctcattaat tactctgtac
     4801 tgccccatgt ccatactgtt gttgtcctta ttgacaactt cgcagggaat aatttcattc
     4861 tggctcacaa gctttctagt tactctgaca actttcagcc tcatgtcctt cacaattttg
     4921 tcatcgggtt ctgccccttc aattctgtca gaatgcgata gattaatcgg ttcgtcccga
     4981 agagcttcct tcaccgtttc ctttgaagtc atcaattcaa tttcacgccc atcggcaaaa
     5041 acataaacag gtactgcact tcttatattt atgatgttat tttctttgct ttgcagtttt
     5101 gtgtcaatcg gaacactgac ataatcatac ttgcccagtc ttattccatt ttgctctaaa
     5161 acttctttta cggtggattt cactgttctg acaaccatcc ggtttccatc aaggtttata
     5221 accacttcct tttgaaaata ggaaacagtc ccaaccatca atgcaataac ggccgcagtg
     5281 attacaactg ccaccatttt caatgaaata caccttttga tttcttttac aagcgataac
     5341 actttcaatc ctcctgttca aacattaagg gacctttagg tattttattc tatataacct
     5401 tctttgtcaa attcccctgt tttgccccgg attaaggtga aggaatataa tgcaaatctt
     5461 tgtcgttact gaagcaccag gttattgtct ttggcataat acaaattaat tatcgtcttt
     5521 atcagaaaaa actataccct tatttaattt atgaaataca taataaaatg aaatcccact
     5581 gacaaaagct ccggggacat cgagccccca tcgctttttg gtcatttcac ttcgtgaaat
     5641 cccactaatt aaaaagagtc aaggaaatgc tccctgactc atttttatta atacatatat
     5701 ctgccgcaca tattgggatt acacgggtat ggacttgcca tctgatgcat tttgtattgg
     5761 tcggccccct ggcaattact catatatggg ctgggtgcca tcccctgacc catagctgca
     5821 tactcatgct gcataaaagg gcactgcatg cttatcaata aagggcaaca cacaatctgc
     5881 ggaaccattt gaggctgctg catttgcggt ggttgaacct gtggctgctg cataattggc
     5941 attggcccca attgaggtat ctgttccggc atcggcatag ctcccatttg cggcataggt
     6001 tgtgcctcag gcatttgagg catttgaggt atctgaggta tctgaggtat ctgctccatc
     6061 ctgttaacac ctgtcatgtc aggtgatatc tcctcaggtt ccttattaaa tttcttctcc
     6121 ataaaaaata tacctccggt ccatattcta cttaaactca atattattat atgtaccgaa
     6181 ggtaatttgg tgataataaa gttatgtcaa ttttattgga ttctaaaaag cctttttgca
     6241 ttgtctgttg tgagttctgc taccttttcg agctcaattc ctcttatggc agaaatcttt
     6301 tgtgcaacca gacttacata gccagagtcg tttctttttc ccctgtgagg ctccggagtc
     6361 aaatacgggc aatccgtctc aatcataagc ctgtccaagg gcacatattc cacaacctcc
     6421 acaagctttt ttgcattctt gaaggtcaat gcaccgccta cagagatata aaagttatta
     6481 tccaaaacct ccctcagcat ctcaacactt ccggagtagc agtgaaaaac cccgccaacg
     6541 tccttcgcct gttcggactt aactatatcc atagtgtcct tgtgagcatc cctgtcatga
     6601 attattatgg gtaaatccaa ctcctttgca agggctatct gtttggcaaa atgaagcttc
     6661 tgcaaatctc tgggcgagta ctcataatag tagtccagac cgatttctcc gattgcaaca
     6721 attttatcgt cttttgtaaa gtctctaaga gctacatatg tattatcgct tatctcactt
     6781 gcacagtggg gatgcactcc taccgcacca taaacaaaat caaatttttg ggtcaaagaa
     6841 acagtatcca ttgatgaagt aatattggag gaagcattca atatatatgc aacaccgctt
     6901 tcaaaagcct tcattatcgt ttcaagcctg tcattttcaa acctttcatc gtcataatgt
     6961 gcatgggaat caaaaaacat gtacgtccct ccct
//
LOCUS       DAOOJC010000072         6969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_9008_length_6969_cov_4.091553, whole genome shotgun sequence.
ACCESSION   DAOOJC010000072 DAOOJC010000000
VERSION     DAOOJC010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9008_length_6969_cov_4.091553"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLA01_03205"
     CDS             <1..254
                     /locus_tag="PLA01_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HPD00340.1"
                     /translation="EVISGVDFIKDFAAGLTNFFGGRSGSYEGELIKAREEALQEMTR
                     RGNAIGANAIVGVDIDYEVLGQGGNMLMVTASGTAVVVE"
     gene            complement(512..1132)
                     /locus_tag="PLA01_03210"
     CDS             complement(512..1132)
                     /locus_tag="PLA01_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPD00341.1"
                     /translation="MYNNYHNYPCAYPAQIPMYNPNNMYNNCNPYPCPYYINPQLYNP
                     CYPKQLPEQFMPLPEKTDCKSPICLKDYGPKPFVVNIEDAAKQNKTFRTALWTGKHLQ
                     LTLMSINAGEDIGLEIHPDVDQFIRIEEGQGLVKMGDKRDKLDFEKKVCDDFAVLIPA
                     GKWHNIINTGKKPLKLYSIYAPPEHPHGTVHKTKADAEKAEKCHSC"
     gene            1615..2028
                     /locus_tag="PLA01_03215"
     CDS             1615..2028
                     /locus_tag="PLA01_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family phosphohydrolase"
                     /protein_id="HPD00342.1"
                     /translation="MSNFIQHGQITCLEHSLYVSYCSYLVCRRLGFNYRSAARGGLLH
                     DFFLYDWHKEIPYKGLHGLKHPYVALENANKYFQLNKMEQDIISKHMWPLTIVPPRYL
                     ETLIVNIIDKYCAFMETFNIGKRRSIIRLQSLLSF"
     gene            2319..3074
                     /locus_tag="PLA01_03220"
     CDS             2319..3074
                     /locus_tag="PLA01_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPD00343.1"
                     /translation="MLLVFITAIGVGGATVVGSLIGFLFGKIPHKYNDAILGFAAGVM
                     LAAAVMGLILPSAEMGGKYGIWITALGIFCGAGFLNIMDKVTPHLHHLSGVDTEKHEH
                     NEALNKILLFVFAIAIHNLPEGLAAGVGFGTENIGNAVTVAVGIALQNIPEGMVIISP
                     MLLAGVSKKRALLIGSATGLVEVVGTFIGYFASSVSGQILPFALAFAGGTMLYIISDE
                     MIPDTHSHGYERMATYSLLTGFVVMLIIDTYIV"
     gene            3236..3625
                     /locus_tag="PLA01_03225"
     CDS             3236..3625
                     /locus_tag="PLA01_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HPD00344.1"
                     /translation="MYEMKPEYYTGIDFIDEEHTKLFAIADEAYELLKNQFIPDKYDY
                     IMNLIEELENYTKYHFRHEEEYMNSIGYKKMLSHKVAHDDFIQKINEFDPDKVDKNPK
                     DTLLELVDFLATWLVLHISKADKLIAK"
     gene            3710..4363
                     /locus_tag="PLA01_03230"
     CDS             3710..4363
                     /locus_tag="PLA01_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HPD00345.1"
                     /translation="MEQKALWNLSYGVYIVSTWDGERPTGCVANSVMQITSEPATVAI
                     SISHDNYTNQCIEKTGKFAIAVLAEDTDPSIIGTFGFKTGRDVNKFDSIGYEIKDGMP
                     IVKDGCAYITCEVIDKMETNTHTVFLGKVSDADFISKGVPMTYAYYHKVIKGKSPKNA
                     PTYIADGGENMEKNTEAKYVCGVCGYEYDGDIPFEDLPEDYICPVCGQPKSVFVKQD"
     gene            4595..4717
                     /locus_tag="PLA01_03235"
     CDS             4595..4717
                     /locus_tag="PLA01_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00346.1"
                     /translation="MKRLSESELEIMLAIWKANEPVKSSYIGDVLSSKGWAKTT"
     gene            5282..6502
                     /locus_tag="PLA01_03240"
     CDS             5282..6502
                     /locus_tag="PLA01_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPD00347.1"
                     /translation="MEEYIIKEKGHPVQSDSWMRKIALFLASQNVSLFGSSVVSFAII
                     WYITLETSSGKWVMLSAICSMLPQVLISLWAGVWADRYNRKYLILFSDGFIALSTLIL
                     SAIFFAGYRSISLLLVVSFIRSIFTGIQTPAVNAVYPQIVPAEKLTKVNGINQTLNAL
                     LLLLAPAVGGMVLGYLDISWAFMIDVITAVIAIIILLFIKIEKVSQPDDKSSVFENLR
                     QGIKYTFENKLLKDIIICFAISFFLITPAAFLTPIMIQRSFGDEVWRLTANEMVWTIG
                     SLIGGIFVSLRGEFKDKVRTIAISLIAFGITFALLGVAKWFVVYLLIMGLSGFFMPIG
                     STAQTVLIQENVQETMMGRVFSIIQIITSASMPVAMLLFGPLADVVSVELILLVTGAM
                     LAGLGVVVSRYKIT"
     gene            6622..>6969
                     /locus_tag="PLA01_03245"
     CDS             6622..>6969
                     /locus_tag="PLA01_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPD00348.1"
                     /translation="MYDLLKSRRSIRKFQDKEVEKEKIDTILRSALLSPSSRSRKPWE
                     FIAVTDKELLQRLSKCREHSSSFLAGAPLGIVVIADPEVCDVWIEDASIASIIIQLAA
                     HSLGLGSCWIQVRD"
BASE COUNT         2090 a         1095 c         1540 g         2244 t
ORIGIN      
        1 gtgaggttat atccggagta gactttataa aagactttgc agccgggttg actaatttct
       61 ttggaggacg ttccggatcc tatgaaggcg aactgattaa ggcaagagag gaagcactcc
      121 aggagatgac aagaagaggg aatgctatcg gtgccaatgc cattgtcggg gttgatatcg
      181 attatgaagt gctcggacag ggcggtaata tgctcatggt aactgcttcc ggaactgctg
      241 tggtagttga gtaattgaaa gcagcctgaa aaatactcat ccttgctcaa gtacgagatg
      301 tattttcgcc agggaaaccc aagggtttcc ctcggtgcat gaaataagct tgaagaaaat
      361 cattccataa acataagacg ttggaatctt tttcacttaa atgacatgct catggtttcc
      421 ctgacgtata gaaggagaaa atggcaaagc ctaatttagg gggagtgaga ggctttcagt
      481 tatatgccgc ttaaattatc ttgtcaggct ttcagcagct atgacacttt tcagctttct
      541 ctgcatcggc tttagtttta tggaccgtac catgtgggtg ctcaggtggt gcgtatattg
      601 agtatagctt aaggggcttt ttgcccgtat taattatgtt gtgccatttt cctgctggaa
      661 tgaggactgc aaagtcatca catacttttt tctcaaaatc cagcttatcg cgtttatcac
      721 ccattttaac aagcccttgc ccctcttcaa ttcgaataaa ttgatcgaca tcgggatgga
      781 tttccaagcc aatgtcttca cctgcattta tactcattaa ggtaagctgt aaatgctttc
      841 ctgtccataa tgcagtacga aaggttttgt tttgctttgc agcatcttca atattaacca
      901 cgaagggttt cggaccgtag tctttcaagc agatgggact tttgcaatct gttttctccg
      961 gtaagggcat aaactgttcg ggaagttgtt tcggatagca agggttatat agttgagggt
     1021 ttatgtagta tggacatgga tacgggttac agttattgta catattattt gggttataca
     1081 tgggtatttg agccggataa gcacatggat aattgtggta gttgttatac attttgatcg
     1141 ttcctttcat gggttttatt tatattttat gcattagatt tatattagtg cctgtggagt
     1201 ttacgtccga gacaattaga tttactataa agcaaaattg atgtaatata tatcatagta
     1261 tgtaaatttt tttatcgcat atttaaatgc accatctttc aaaaagcttg tttacaataa
     1321 tgaaaaatat atatacaata tataatatag gtgtaaaaat agtacgatat aatataaagg
     1381 tatgaataat tatcaccaaa gggtataagg catgtgagcc cgaatttgtc gggaataaag
     1441 gttgtaatag attttcgaag accgtaagta tgaaagttta ttacactata gtgaggagca
     1501 atttctataa aagcttggtt aatttcaaaa ttcgtactcg gacagaatga ttctgttgat
     1561 atagagtatc tcaattgcat acgtgatttg attgataacc aaagggtaag aagcatgagt
     1621 aactttatac agcatggcca aattacctgt ttggagcata gcctctatgt ttcatactgc
     1681 agttatcttg tttgcaggag gctggggttt aactaccgtt cggcagccag gggaggtctt
     1741 ttgcacgact tctttttata tgactggcac aaggagatac cgtataaggg cttgcatggt
     1801 ttaaagcatc cttatgttgc attggaaaat gctaataaat atttccaact gaataaaatg
     1861 gaacaggata ttatatcaaa gcatatgtgg ccgttgacaa tcgtgccgcc cagatacttg
     1921 gagactctca tagtaaatat tatagataaa tattgtgcat tcatggagac ctttaatatc
     1981 ggtaaacgaa ggagtatcat cagacttcaa agtttgttgt cattctgaac aggaaaaaag
     2041 ttcacaaatt cttatcacca tatcaaaact ttatttattt tctctccgtt tttgtgctgc
     2101 ttttgcatag agattaaatt cattatgtgt agagcagtag aattctgtgt caataggcat
     2161 agtaatgagt atatcctgct caactccgga tcttaataga atatataaat atcttccaaa
     2221 taattattga caataaaggt ttaatgggtt aaaacagtgg tatacaatta aagcatgttg
     2281 tatatttatc ataacttttt aaaacaaagg tgaaggatat gcttttagta tttataacag
     2341 caatcggagt cggcggagca acagttgtcg gctctcttat agggttttta tttggtaaaa
     2401 taccgcataa atataatgat gccatattgg gatttgccgc tggtgttatg ctggcagctg
     2461 cggtgatggg acttatttta ccttctgccg aaatgggcgg aaagtatggg atatggataa
     2521 ccgctctggg gattttttgc ggtgccggat ttttaaatat tatggacaag gtaactcctc
     2581 acctgcatca tttgtcggga gttgacactg aaaagcatga gcataacgaa gctctgaata
     2641 aaatattgtt atttgttttt gctattgcaa ttcataattt gcccgaggga ttggcggcag
     2701 gtgtaggttt tggaacggaa aacatcggta atgcggttac ggttgccgta ggtattgcat
     2761 tacaaaatat accggaggga atggtaatta tctctcccat gcttcttgcc ggagtcagta
     2821 aaaaacgtgc ccttttaatc ggctcggcta cgggtttggt agaggttgtg ggaaccttta
     2881 tcggttactt tgcttcttct gtttccgggc agatactgcc cttcgcatta gcctttgcag
     2941 gcggtacaat gctttatatt atcagtgatg agatgattcc cgatacccat agccacggat
     3001 atgaaaggat ggcaacatat tcactgctca caggatttgt agttatgctg attattgata
     3061 catatatcgt ttagaggatt atttttaagg cattcggttt atcctgactg gtttgacata
     3121 aaatatatag ggtattgatg ctcatatata agcggattgg tgacgaaata attaatgtgg
     3181 ccggatattt caaaaaatcc gtcggcatat atgctataga caaggagaga tgaatatgta
     3241 tgaaatgaaa ccggagtatt atacaggaat tgattttatt gatgaggagc atacaaaact
     3301 gtttgctatt gcggatgagg cttatgagct tttaaaaaac cagtttatac ccgataaata
     3361 tgactatata atgaatttaa tcgaagaact tgaaaattat actaaatatc atttccggca
     3421 tgaagaggag tatatgaaca gtatcggtta taaaaaaatg ctctctcaca aggttgccca
     3481 tgatgatttt atacagaaaa taaatgagtt tgaccctgac aaagtcgata aaaacccgaa
     3541 ggatacatta ttggaattgg tggatttttt ggctacatgg cttgtactgc atatatcaaa
     3601 agcagataaa ctgattgcaa aataataaaa atatgatatt attttattgt gtgtggtaac
     3661 ttatgccacc agcgaataac tatatcaatt gtacaaggat ggtatgacta tggagcagaa
     3721 agcattatgg aatctttcat atggagtcta tatcgtctca acgtgggatg gagagcgtcc
     3781 taccgggtgc gtggccaaca gtgtcatgca gattacatcg gaacctgcaa ctgtcgccat
     3841 cagtattagc catgataact ataccaacca gtgcatagaa aaaaccggaa agtttgccat
     3901 cgctgtatta gcagaggata cggatccttc aattatagga acctttggat ttaagaccgg
     3961 cagggatgtg aataaatttg attccatagg gtatgaaata aaagacggaa tgccgattgt
     4021 caaagatggg tgtgcatata ttacctgtga ggttattgat aagatggaaa ccaacaccca
     4081 tacggttttt ctgggaaaag tgtcggatgc ggattttatt tcaaagggtg tgcctatgac
     4141 ttatgcctat taccataagg ttattaaggg aaaaagtcct aaaaatgctc cgacttatat
     4201 tgcagatgga ggtgaaaaca tggaaaaaaa tacagaagcg aaatatgttt gcggtgtgtg
     4261 cggttatgaa tatgatgggg atattccttt tgaagatttg ccggaggatt atatctgccc
     4321 cgtgtgcgga caacccaagt cagtgtttgt aaagcaagat taaattaaaa atgtcctatt
     4381 agtttttaat tagcagaaaa tcaatcgtca tgtaattaag gtgacggttg attttttgtt
     4441 tttataatta acaccttgac atctatattc tacaaatgta gaatataatt aaatatttta
     4501 catgtgtaga acaaaacaaa aagggattga attatcatga taatggcaga actattgatg
     4561 ctctacggat caggagaaaa aggggaggat aagtatgaaa agactgtctg aaagtgaatt
     4621 ggagattatg ctggcgatat ggaaggccaa tgagccggtg aaatcctcgt atattggaga
     4681 cgtcttgagc agtaaaggct gggccaaaac cacgtaattc cataaaggac atggtggcag
     4741 aactgtatca aaactaagta taacagtctt ttcaacacct attttgattt tagcttgcat
     4801 gccttgacat tttatgatga agaactagat cgtcaagtaa atttattaat tgattatgag
     4861 tttacgtatc cccgggaaac ttatttgatg catgttcaca ataaaagtat tgataaccgt
     4921 ttattcatgt ggtatacttt ataacaacta tttgttatta aaatattaaa gaaagaggat
     4981 tacgaatgtt tgttgttaga atgaggatta attattttgc ttaatgcaaa taactaaata
     5041 ggagataagg taaaactcta ttgttatagc ttttgtttgt gttgccttgt tttgaaattc
     5101 ttacaatgaa cattcatgca cttttcgtat ctttaatatt ttaagtagac tgtattatac
     5161 gtagtttata acgtatagtt attaaaatga atatttaata aagatatagg gttgcagatg
     5221 atgcatgttc atttgcagcc ctttttaatt ttaataatac tgactatgag gtgaaaaaca
     5281 tatggaagaa tacattataa aagaaaaagg acatccggtt caaagcgatt catggatgag
     5341 aaaaatagct ttgtttttgg ccagccagaa tgtttcgctg tttggatctt cggtggttag
     5401 ctttgcgatt atatggtaca ttacgcttga gacatcttcc gggaagtggg ttatgctttc
     5461 ggctatttgt tccatgctgc cccaggtatt gatttcactt tgggcaggtg tatgggccga
     5521 tcgatacaac cgcaagtacc tgatattgtt ctccgatggt tttattgcat tatcaaccct
     5581 gatactgtcc gccatatttt ttgcgggata tagaagcatt agccttctat tggtggtatc
     5641 cttcatccgc tcaattttta cggggattca aacaccggcg gttaatgccg tttatcctca
     5701 gattgttcct gcagaaaagc ttacaaaggt gaatggaatc aatcagacat taaatgccct
     5761 attgctttta ttggctccgg cagtaggggg tatggttctt ggctatcttg atatttcatg
     5821 ggcatttatg atagatgtga taactgcggt aatagctatt attattctgt tatttataaa
     5881 aattgagaag gtttctcagc ctgacgacaa atcctctgtt tttgaaaacc tgcgtcaggg
     5941 tattaaatat acctttgaaa ataaattgct gaaagatata attatttgct ttgcaatttc
     6001 ctttttcttg ataaccccgg ctgcgttctt aacacctatt atgattcaaa gatcttttgg
     6061 cgatgaggtg tggagattga cagcaaatga aatggtctgg actatcggtt cgctgattgg
     6121 aggaattttt gtttcgcttc gaggagagtt taaggataag gttcgtacca tagcaatttc
     6181 tcttatagcc tttgggataa cctttgctct tttgggagtg gcgaaatggt ttgtggtata
     6241 tcttttgatc atgggacttt ccggcttctt catgccgata ggctcaacgg cacaaactgt
     6301 gcttatccag gaaaatgtcc aggagacaat gatgggacgt gttttctcaa ttattcagat
     6361 tattacatct gcatccatgc ctgttgccat gctcttgttc ggaccgcttg cagacgtggt
     6421 atctgttgag ttgatactgt tggtaacggg agctatgctt gccgggctgg gagttgttgt
     6481 atcaagatac aagataactt gagttacctt ggcaccggat gtaagaaaat ttttctacaa
     6541 atgaaaaatt ttctttgaac gaagtgttat aatatttagt aataaaacag gtgcagcgaa
     6601 aacacaagga ggaatgtttt tatgtatgat ttgttgaaat cgagaaggag cataaggaaa
     6661 tttcaagaca aggaagtaga aaaggaaaaa atagacacaa tattaagaag tgctttgctt
     6721 tcaccttctt cccgctcaag aaagccctgg gagtttattg cggtaaccga taaagagtta
     6781 ttgcaaaggc tttcaaaatg cagggaacac agctcatcct ttttggccgg tgcaccattg
     6841 ggcattgttg ttattgcaga ccctgaagtg tgtgatgttt ggatagagga tgcctcaatt
     6901 gcgtcaatca taattcagtt ggcggcacat tctttggggc tgggttcatg ctggatacag
     6961 gtgagggat
//
LOCUS       DAOOJC010000073         6961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_9021_length_6961_cov_3.853606, whole genome shotgun sequence.
ACCESSION   DAOOJC010000073 DAOOJC010000000
VERSION     DAOOJC010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9021_length_6961_cov_3.853606"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="PLA01_03250"
     CDS             complement(<1..1334)
                     /locus_tag="PLA01_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00349.1"
                     /translation="MKKGLLCFVAIIIFVALGQAAYAQPAFIWEKNFDLQTCNFYAVC
                     FGNNTFVAVGGDGAIKVSRDGDNWSRIGSDLYSDLFDVIYADGKFVAVGGAGTIITSQ
                     DGVNWTKVKTEVKNGILRIHWNGKQYLAATRSNLIISSDLKTWEELPLDSSEIASIAY
                     GNNKYVIVKRDGKILTSTDGYEWKEKYTLQWCSFSDVIYNGNMFVAVGTNMITLSFGV
                     QTEGQSVIMTSPDGENWHIQDVECEAGLDGITWNGEKFVITGSDIILSSEDGNKWDAV
                     DVNAYLEDVAFGNGVFAAVGYNGVIISSNDGDYWETKVEAESYDINDVIWHDNRFIAA
                     GEGNASSILVSEDGEKWTATGPKEDTFFSDAIAWDGEKYVVLGIGSKILTSPDGVSWQ
                     MHNMDKNLYNEHPHYNSMVWDGEKFVVVGHEGLISISSDGLHWTEINSGFTD"
     gene            complement(1381..2364)
                     /locus_tag="PLA01_03255"
     CDS             complement(1381..2364)
                     /locus_tag="PLA01_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00350.1"
                     /translation="MNNGIRIPGFPPPEKQDSVKFYVLKSISYSTRMIIYLLFIAFGF
                     LIQYVMMNAWPGAIFLICATVLNLVRGYDSRARLKAFEPDTQWTTVDMGRIYEIENLE
                     KKAKKWDRDVLDISNGLGAFVFILLILGLFILSSVLSGFYEFAEVRSILLTDTIILIL
                     PMWFNGIRRILTQNNLRIKIDIIKSVEEYFRTVKLEGEHFKPALMLARDKTGKSMPKD
                     VRFTITFDDMPADFYGIQAQININLVQGTGYPYFYCVIPAKTGFGLREHLNKIPRDKN
                     VIIEFQHDVQAEVIVIRQYTTQTSGYHTKPKSCRYILAVAIHAARMILKDK"
     gene            complement(2380..3693)
                     /locus_tag="PLA01_03260"
     CDS             complement(2380..3693)
                     /locus_tag="PLA01_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD00351.1"
                     /translation="MNKQKLSWVDEFLTRIKPYVYMRLTDNVLIRMPNEAFKLNATGA
                     RIIDHILKGGSVYDFIKVRSDFPDTGEQLERFFTDFLRVWNREVCENYTTAAIHRVEF
                     STGYIELPILSEVAITYACNIKCRFCYAGCTHQKKQKELDTEGFKKVLDIIRHEAQVP
                     SVSFTGGEPLLNKDLPELIKYASKVNGMRVNLITNGTLITPEKAEEFAEAGLSSAQVS
                     IESPYASVHDSITKVPGSHEASVAGFKALKAAGITVHPHFTVCKLNRESAEKYPEFCK
                     EMGSDRFSANLVIPAGRGDDDDLTVSYSDIGEIISKLRKKASETGVKFMWYSPTPLCL
                     FNPIAAGFGNKGCSACEGLLSVNPEGNVLPCSSWSEPMGNLLEEGFETVWFNKRSKGI
                     RNKEAAPEECKSCKHFAVCQGACPLYFNIHGCGELHSAWKSFGLC"
     gene            complement(3690..5402)
                     /locus_tag="PLA01_03265"
     CDS             complement(3690..5402)
                     /locus_tag="PLA01_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00352.1"
                     /translation="MKKIIIVFLTLFVLLIPFPVEVNASSIANLGFVETDVILQPDGN
                     AIVYYTVRYNLVPGKAMMAFTMSGFDRLNPVFDRENAWVITDDNTSYPIDIVNLGGGK
                     YDIINAGEKRYGGDYLTYKFRFAANMAKAGYLEKTTSDDGRRLMVFNWAPTEWDEPME
                     HYTVRINYPLAFPAESGTREEVEQFLLENDFLTEQWMNDRYLIDYRVQMLNETPRVQV
                     QLHRENLPARYSFKIQQFISEDIFDIDDTVTGYDKGKHEDELEDELEEGRNDWQPQDF
                     GNSKGKSFGRTALVVVMIGLFAVTVLAAGKKHRSMVRAQATLDEIQWARDDWEPPKLE
                     IASFRKDGTIAEGLDGVEIALLIGIPYKRILSVILSKLIDQGFLEEVSRDPLRVRRIE
                     HADPSSVLNEYELMMYEAARDGEFEEREIEGILQRLVDNVQNKTWDCDIEATRKYYND
                     KIAEVLKNKNIDLNRENQEVYSDEYYEDSWPSWYYYHSGVSYHRYRDYYNRYEVSVPA
                     NPDRLKFNSVIDSNKGVFACHSACHDACHDACHSACHDACHSACHSACHSACHSACVS
                     GGAR"
     gene            6261..6961
                     /locus_tag="PLA01_03270"
                     /pseudo
     CDS             6261..6961
                     /locus_tag="PLA01_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
BASE COUNT         2034 a         1655 c         1222 g         2050 t
ORIGIN      
        1 tcatctgtga aaccggaatt aatctcagtc cagtgtaaac cgtcggatga tattgaaata
       61 agtccttcat gccccacaac tacaaatttt tcgccatccc acaccatact attatagtga
      121 gggtgttcat tatagaggtt cttatccata ttatgcatct gccagcttac gccatctggg
      181 gatgtcaata ttttactccc tatacctaat actacatact tttcaccatc ccaggctatt
      241 gcatccgaaa aaaaggtatc ttcttttgga cctgttgcag tccacttttc accgtcctct
      301 gacaccaata tggatgacgc attaccttct ccggcagcaa taaaacggtt atcatgccat
      361 attacatcgt taatatcata gctttcagct tcaactttcg tttcccaata gtcaccgtca
      421 ttggaagaaa taataacgcc attataacca acggctgcaa acactccatt tccaaaagca
      481 acatcttcaa gataagcatt aacatcaaca gcatcccact tgtttccgtc ttccgatgac
      541 aatataatat cactgcctgt aataacaaat ttttctccgt tccatgttat gccatcaagt
      601 cccgcttcac attctacatc ctgaatatgc caattttctc cgtctggtga agtcattatt
      661 actgattgac cttcagtttg aacaccaaat gacaaagtta tcatatttgt tcctacagcc
      721 acaaacatat ttccattata aataacatca gaaaagctgc accattgcaa agtatatttt
      781 tccttccact cgtatccatc ggtcgatgtc agtatcttgc catctctttt gacaatcaca
      841 tacttattat tgccatatgc aatggatgcg atttccgatg aatccaaagg cagttcttcc
      901 catgttttta agtcggaaga gatgattaag ttgcttctgg tagcggccaa atactgcttt
      961 ccattccaat gtattcttaa aatcccgttt ttcacttctg ttttaacctt cgtccagttt
     1021 acaccgtcct gagaagttat aatagttcca gcaccgccaa cagccacaaa cttgccatcg
     1081 gcatatataa catcaaacag gtcagaataa agatccgaac caattctcga ccagttatcc
     1141 ccgtctctcg atacctttat ggcaccatcg ccacccactg caacaaatgt attatttcca
     1201 aaacaaacag catagaaatt gcaagtctgc aaatcaaaat ttttctccca aataaaagca
     1261 ggctgtgcgt atgcagcttg acccaaagcc acaaaaataa taattgcaac aaagcataaa
     1321 agtccctttt tcataattat tatcactcct tcctccaacg gaataccaaa acataaaaat
     1381 ctatttgtct ttaagaatca ttcttgcggc atgtatagct acagcaagaa tatacctgca
     1441 gctctttggt tttgtgtggt accccgatgt ctgtgttgta tactggcgaa taacaattac
     1501 ctccgcctgt acgtcgtgct gaaattctat aataacattt ttatccctag gaattttatt
     1561 gagatgctcc ctgagcccaa accccgtttt agccggtata acacaataga aataagggta
     1621 acctgtaccc tgcaccagat ttatatttat ctgggcctga ataccgtaaa agtcagccgg
     1681 catatcatca aaagtaatag taaaacgcac atccttgggc atgctcttcc ctgttttatc
     1741 ccgggcgagc attaatgcgg gtttaaaatg ttctccttcc agcttgacag ttctaaaata
     1801 ctcttccacg ctttttataa tatcaatttt tatacgcaaa ttattctgcg tgagaattcg
     1861 ccgtattccg ttaaaccaca tgggtaatat aaggattatt gtgtcggtca gtaaaatact
     1921 tctcacttca gcgaattcgt aaaagcccga caaaaccgat gacaatataa acaaccccag
     1981 tatcagaaga ataaacacaa atgctcccaa gccgttgctt atatccaaaa cgtcccgatc
     2041 ccattttttc gcttttttct ccaggttttc aatctcatat atgcggccca tatccaccgt
     2101 cgtccactgt gtgtcgggtt caaaagcctt aagccgtgca cggctgtcat aaccccgaac
     2161 cagattcaaa acggttgcac atattaaaaa gatagctccg ggccaggcat tcatcatgac
     2221 gtactgaatc aaaaagccaa aggcaataaa cagcaaatat ataatcattc tggtagaata
     2281 tgaaatactc tttaacacat aaaatttcac gctgtcctgt ttttccggag gtggaaaacc
     2341 cggtatacga atcccgttat tcatactgta ctcctttctt taacatagac cgaaggattt
     2401 ccatgcactg tggagttccc cgcatccatg tatattaaaa tataagggac atgccccctg
     2461 acacaccgca aaatgtttac agctcttgca ttcttcaggt gctgcttcct tgttccgtat
     2521 ccctttagac cttttattaa accatacagt ctcaaagcct tcttccagaa gatttcccat
     2581 cggctctgac cagcttgagc acggcagtac attgccttca ggattaacag aaagtaatcc
     2641 ttcgcaggca ctgcatcctt tattcccaaa ccctgctgct ataggattaa acaagcataa
     2701 gggtgtgggc gaataccaca taaattttac gcctgtctct gaggcttttt ttcgtagctt
     2761 cgatatgatt tcacctatat cactgtagct aaccgtcaaa tcatcgtcat ctccacgccc
     2821 tgcaggtata accagattag cagaaaacct gtcggagccc atctctttac aaaattcagg
     2881 atacttctcc gccgattcac ggttcaattt acagacggta aaatgaggat ggactgtaat
     2941 tcccgctgcc tttaaggctt taaaccctgc tacggaagcc tcatgggaac caggtacttt
     3001 tgtaatggaa tcatgcacag aggcatatgg cgattcaata ctaacctgtg cagatgaaag
     3061 gcctgcctcg gcaaactctt cggctttctc aggagtaatc aatgttccgt ttgttatgag
     3121 attaaccctc ataccgttta cttttgaggc atattttata agctccggaa ggtctttgtt
     3181 tagaagcggc tcccctcccg taaaggatac actcggcacc tgtgcctcat gccttattat
     3241 gtcgagcacc tttttaaacc cttcggtatc aagctccttt tgcttttttt gatgagtaca
     3301 tcccgcatag caaaaacggc attttatatt acaggcataa gtaattgcca cttccgaaag
     3361 tataggaagt tcaatatagc cggtactgaa ttcaacccta tgaattgcag cagttgtata
     3421 attctcacac acttcacgat tccatacacg taaaaagtct gtaaaaaacc gctccaactg
     3481 ctccccggta tcaggaaaat cggaacgcac cttgataaaa tcatacaccg agccaccctt
     3541 taaaatgtgg tcaataattc ttgcaccggt ggcattaagc ttaaacgcct cattgggcat
     3601 tcgaatcagc acattgtcag taagccgcat gtatacatac ggctttatcc gggtcagaaa
     3661 ttcatcaacc caggataatt tttgcttatt catcgtgcac ctccacttac acaagccgag
     3721 tgacacgcag agtggcaggc tgagtgacat gctgaatgac aggcatcatg gcaggccgaa
     3781 tgacaggcat catgacaggc atcatgacag gctgagtgac atgcaaatac gcctttattc
     3841 gaatcaataa ccgaattaaa cttcagtctg tccggattag caggtaccga tacctcatat
     3901 ctgttatagt aatccctata tctatggtag cttactccac tgtgataata ataccaggaa
     3961 ggccaactgt cctcatagta ctcatcacta tatacctctt ggttctccct atttaaatcg
     4021 atattcttat ttttaaggac ttcggcaatc ttatcgttat agtatttgcg ggtggcttct
     4081 atgtcacagt cccatgtctt gttctgtaca ttatccacca gcctttgcaa aatgccctct
     4141 atttcccttt cttcaaattc cccgtccctg gcagcttcat acatcatcaa ctcatattca
     4201 ttaagaaccg atgacggatc ggcatgctca atacggcgta cccttaaggg atcccgggaa
     4261 acttcctcaa gaaatccctg atctataagt ttcgagagaa taaccgaaag aatcctctta
     4321 taaggaatcc ctatcaaaag ggctatctca acaccatcca acccttcggc aatggtaccg
     4381 tcctttcgga acgaagctat ctcaagcttt ggcggctccc aatcatccct tgcccattga
     4441 atctcatcca aagtagcctg agcccgcacc atggaacggt gttttttccc cgcagctaaa
     4501 acagttactg caaaaaggcc tatcataaca accactaaag ctgtcctgcc gaaagatttg
     4561 ccttttgaat ttccaaaatc ctgcggctgc caatcattcc gtccttcttc caattcatct
     4621 tccaattcgt cctcgtgttt ccctttatca taacctgtta ccgtatcatc tatatcaaaa
     4681 atatcctcag aaataaactg ttgaatttta aagctgtatc ttgccggtaa attctccctg
     4741 tggagctgca cctgaacccg gggcgtttca ttcagcattt gcaccctgta gtcaataagg
     4801 tatctgtcat tcatccactg ctccgtcaaa aaatcatttt ccagcaagaa ttgctccacc
     4861 tcttctcttg tacccgattc tgcagggaaa gcaagaggat aattaattct aacggtatag
     4921 tgctccatgg gttcatccca ttcggtagga gcccaattaa acaccataag cctgcgtccg
     4981 tcatccgatg ttgttttctc cagataacct gcctttgcca tgttagctgc aaaacggaat
     5041 ttgtaggtga ggtaatcacc cccatacctt ttttcgccgg cattgataat gtcgtattta
     5101 ccgccgccaa gattaactat gtcaatgggg taagatgtgt tgtcatcagt aataacccat
     5161 gcattttcac ggtcaaaaac aggattcagg cggtcaaacc ccgacatggt aaatgccatc
     5221 atagcctttc ccggcaccag attatagcgg acagtataat aaacaatggc atttccgtca
     5281 ggctgcaaaa ttacgtcagt ttccacaaaa ccaagattgg caatacttga agcattaacc
     5341 tcaacaggaa aaggaataag taagacaaac agagttaaga aaacaattat tatttttttc
     5401 ataattagca cctttattat tcgtataaat tttttccatg cgtattttaa aataaaatga
     5461 cttttacatt aatccctact tttaagtata aaagtttttt ccaccgtgtc aatactttat
     5521 ccggagtaca tgggtgtata ttttttaagt agaaaaataa tatggaagaa aaattgtgaa
     5581 gtgaaattaa gtttttggaa tattattgtt gacattattt aatgcaacca tagtaaatat
     5641 atttattgta taattataca gccgatgata taataacctt tgtaaacagc acttgcaagg
     5701 tatattactt cgatatagga taaaaataaa tatcccgtta tttaagatac taattttgca
     5761 gcattacttt actgctttta tatattgcac caatatacta attctgtgga aacgatgcct
     5821 taaacaaaca atgcagtata tcctttcaat tcatccgatc aacagattta ttatcagggc
     5881 tttatcattt atccggataa cttgtaagtt ctgcaaaaca aatattacaa atatagtatt
     5941 ttatggggag gttaaaagtc atgaagaaaa tgtcaaaaca agtcttggct gttatcatgg
     6001 ttcttgcact ggcaatgtct tttcttccaa caacggttgg tgcagttgaa cccgataatt
     6061 cgcccggggt gaatgattct cttgacacag caattctttc tgaatcgtct tattcaccca
     6121 gtgtttccga gtcggtttat tccgatgtaa tagttcccac tgccgttaag ccatctgttg
     6181 gcggtgcttt gcaagtcctt gaggtggacg gcaaaaaaac cctgtgcggt gaaaacggaa
     6241 atccgattca actcaggggt atgagtaccc acggtttgca atggtttccc gagatcctta
     6301 acgacaacgc ttttgcagca ttgtccaatg attgggacag caatgtggtt cggctcgcca
     6361 tgtatgttgc agaaaacgga tacgaaaagg atccggagct tataaagcaa agggttatag
     6421 acggaatcga tttagccatc gcaaacgaca tgtatgtaat cgttgattgg cacgtactta
     6481 gccctggaga tcccaatgct gaagtgtaca gcggagctat ggatttcttt gacgaaattt
     6541 ccgatcttta tccgaataat aaatacatta tctatgagct ggcaaatgag cccaacggca
     6601 ctgatcccgg tgttcccaat gatgcttcag gctgggaagc tgtaaagtct tatgcagaac
     6661 ccattataga aatgcttagg gataagggaa atgagaacct cattatcgtt tgaagtccca
     6721 actggagcca gcgtgccgac ttggctgccg acgatcccat agatgatgaa aacaccgtat
     6781 atacggttca tttttatacc ggtacccata tgcctgcttc agacagttca gacaggaata
     6841 atgttatgag caacgccatc tacgcaattg aaaagggcct tgtccttttt gcctccgaat
     6901 ggggtaccag cgaagcaacc ggccacaatg gaccttattt ggcagaagcc gatgtgtggc
     6961 t
//
LOCUS       DAOOJC010000074         6864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_9191_length_6864_cov_3.640623, whole genome shotgun sequence.
ACCESSION   DAOOJC010000074 DAOOJC010000000
VERSION     DAOOJC010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6864
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9191_length_6864_cov_3.640623"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PLA01_03275"
     CDS             <1..768
                     /locus_tag="PLA01_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPD00353.1"
                     /translation="ENIPVIFRPFHEAEGYNNTDGSGAWFWWGSGGAEVFKELWKLLY
                     TTLTEEYGLHNLIWEVNLYTYANSFEWYPGDEYVDIVAYDKYEGSPYKWGTSAASTIF
                     LTLVDYTNDTKMVAMSENDTIPDIQNIVNEGAWWLYFCPWYGDFILSSSKNDPKLLNT
                     IYNSEYVISLDELPKDLYEYDDSPAFTYGDLDGDSEINSTDLTMLKRFILKKLKELGV
                     PEEAADLDGDGEITSTDLTILKRFILRKIKDFPVNQK"
     gene            complement(940..1701)
                     /locus_tag="PLA01_03280"
     CDS             complement(940..1701)
                     /locus_tag="PLA01_03280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD00354.1"
                     /translation="MSTVEEKLAKSLTAESTELIPYLPYLLQDLWELGSSPRDIIEMT
                     SKNIQVSKKTRVLDLACGKGAVSVYLAKALGCSVKGIDIIPEFIEFAVNKAKELGVEK
                     LCRFEVGDINEAVKEEKDYDIVILGAAGDVLGNPKETVEKLTRTVKSGGYIFIDDAYG
                     KDDFDDKYLTREKWLKIFESLGVKLLDERIVQEDELKSINDEQQSFIIKRANELKKRY
                     PDKVYLFDSYIESQQAECDELENEITGVTMLLQVV"
     gene            complement(1826..3067)
                     /locus_tag="PLA01_03285"
     CDS             complement(1826..3067)
                     /locus_tag="PLA01_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00355.1"
                     /translation="MKKEDFKNSFDRIKPSESAKKRMLDNVLNNSDRRKGVFVTLFNF
                     KKAIPALVLTAVIVGGLLTYNHMSKKHDRGSSLTYGVENDVSMGREDAIAPLLNQFQI
                     DDRHYIILSDELRAEYGLPTAVIESDIGGKIADITAGPDKSLIGNEVYSYIPAGGESV
                     VAVKKDDEYQLFRFFTFESYNNNQDEDSVRYLALFGIDRADDIAKIQFIGHSEQSKLQ
                     GRTDILGEITDRDEIERFYSFYSVLKNSSDKYFDSIFNYRNTDSGKGIEIDNVQPDRI
                     DPPDAVEPDMTAPDQIDRSEHRPMDQASDGTGVAEDLPLEMPEKGEVGAVSGDTPIAN
                     EVSQSNTSDGMMDMGNTGSGVSHAGQGSAGAALENLVTIRIYNKKGVYFDSPYYINID
                     FISRYEVSEDFADFLGKYIDK"
     gene            complement(3064..3555)
                     /locus_tag="PLA01_03290"
     CDS             complement(3064..3555)
                     /locus_tag="PLA01_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPD00356.1"
                     /translation="MQSSLLRTNEELLEIYERHVNTVYRICFMFMKNRSDTEDMVQNT
                     FIRLMKDKTVFQSEEHEKAWLIRTATNLCKDHFKNWWSKTVGFNHVEEEAVEQPINID
                     GVLKKVMELPAKYKAAIYLYYYEGYSTVEIAKILEKDPSTVRGYLHTGRKLLKMEMEG
                     DFE"
     gene            complement(3952..4932)
                     /locus_tag="PLA01_03295"
     CDS             complement(3952..4932)
                     /locus_tag="PLA01_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPD00357.1"
                     /translation="MVQKFKKKKSRKSIFITFLLIAGITFFVCKYIVIPENSNILEEE
                     YGNKILPNLSSDTIPKPSVSISSDKLSSSNAILIRLDDCAVLMQKNSEERIYPASLTK
                     IMTAIVAIENLPDLKEEIKLTDSMFQGLYEADASMAGFQPGERVRVIDLLYGAILPSG
                     AECCIGLAERIAGSERNFVEMMNQKAKELGMNNTHFENSTGLHDENHYTTVKDLSVLL
                     SYALQNEIFREIFTSSRHSTRPTNIHPDGITYYSTMFEKLKFKNQNIIDGEILGGKTG
                     YTDKAGLCLASLAKVGKHEYILITAGAKGNQHSEQYNITDAIAVYNGIDE"
     gene            complement(5065..6189)
                     /locus_tag="PLA01_03300"
     CDS             complement(5065..6189)
                     /locus_tag="PLA01_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPD00358.1"
                     /translation="MKNNVNSLLKVFFIISAVILFCLGTVRLVDEGFNGIIKEWIYNK
                     FIIEPEHLGQGKIAIIFKSWYEIKQFIIYAFVAFIAFLALCMGLSTYLYSRYKRKKQI
                     SFITGVMDSFMTSGTDDLTLPKEYSEIERQLLKIKSVAQKHQQLMQMEMQRKNDLITY
                     LAHDLKTPLASVIGYLSLLDEASDMPKEQRSKYTVIALEKAYRLEELINEFFEITRFN
                     LQSIVLNKSNIKLAFMLQQLADEFYPMLAPFRKQITINAPEDLVIAGDGDKLARVFNN
                     ILKNAISYSYENSIIDITADRQEDKVIVTFTNQGDPIPSQKLETIFEKFYRLDSARST
                     NTGGAGLGLAIAREIVTAHDGEISVESNAEKTTFTVKLPI"
     gene            complement(6179..>6864)
                     /gene="vanR"
                     /locus_tag="PLA01_03305"
     CDS             complement(6179..>6864)
                     /gene="vanR"
                     /locus_tag="PLA01_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HPD00359.1"
                     /translation="LVVDDEQSIADLIEVYLNNEGFTVYKSYNGKDALRYVESNQLEL
                     AILDVMLPDIDGFTLCRKIREKHNFPVIMLTAKEEEIDKITGLTLGADDYVTKPFRPL
                     ELVARVKAQLRRFTKYNSAELLQEENIIQFAGLVINKDTHECTLNERKLALTPTEFAI
                     LWVLCSNRGHVVSSEELFREVWGDKYFSNSNNTVMVHIRHLREKMNDSKEHPKYIKTV
                     WGVGYKIEK"
BASE COUNT         1973 a         1509 c         1132 g         2250 t
ORIGIN      
        1 gaaaacattc cggtaatttt ccgtcctttc catgaagccg aaggctacaa caacactgac
       61 ggttccggtg cgtggttctg gtggggttcc ggaggtgcag aagttttcaa ggaactctgg
      121 aagcttcttt acacaaccct gacggaagaa tacggccttc ataatttaat atgggaggta
      181 aatctttata cctatgcaaa ttcctttgaa tggtatcccg gtgatgaata cgtagatatc
      241 gttgcgtatg acaaatatga aggttcacct tacaaatggg gtacaagtgc tgcttcaaca
      301 atattcctca ccctcgtgga ttacacaaat gacaccaaga tggttgctat gtctgaaaat
      361 gataccatac cggacattca aaacatagtc aatgaaggag cctggtggct gtatttctgc
      421 ccctggtacg gtgattttat tctcagttcg agcaaaaatg accccaagct tttaaatacc
      481 atctacaaca gtgaatatgt aatcagtctt gatgaacttc caaaagacct ttacgaatac
      541 gatgattcac ctgcttttac ttatggtgat ttggacggag acagtgaaat caattccacc
      601 gatcttacca tgttgaaaag atttatactc aaaaaattaa aagaacttgg tgttccggaa
      661 gaagcggctg atttggacgg agacggtgaa attacttcaa ccgatcttac tatattgaaa
      721 agatttatcc ttagaaaaat aaaagatttt ccagttaatc aaaagtaacg ggttcaactg
      781 taatgtatga aagaagtatc cttaatggtt caaaggatac ttcttcttgt atcagtctgc
      841 aaaatcatgt tttgcatgcc ggtagccatt tataaactcg agatgcattg ttataacttc
      901 caaagttgaa gatttgctgc atagcccaat atttcaaagc taaacaacct gcaaaagcat
      961 cgtaacgcct gttatttcat tctccaattc atcgcattca gcctgctggc tttctatgta
     1021 gctgtcaaaa agatatacct tatccggata tctttttttc agttcattgg ccctctttat
     1081 tataaatgac tgctgttcat cgttaatact ttttagttca tcttcttgaa caatcctttc
     1141 atcaagaagt ttcactccca aactttcaaa aatcttaagc catttttccc ttgtaagata
     1201 tttatcgtca aaatcatcct ttccataagc atcgtcaata aatatgtaac caccgctttt
     1261 aactgttcgt gttaactttt ccactgtctc tttcggattg cccagcacat ctccggcagc
     1321 accaaggata acaatatcgt aatccttttc ttctttgact gcttcgttta tgtctccaac
     1381 ctcaaaccgg cacagtttct caacacctaa ctcttttgct ttgttaactg caaattcaat
     1441 aaactcagga ataatgtcaa taccctttac cgaacagcct agtgccttgg ccaaataaac
     1501 acttacagct cctttaccgc aagccaaatc gagaaccctc gttttcttcg atacctgtat
     1561 attcttcgaa gtcatttcaa taatgtccct gggtgatgaa cccaattccc acaaatcctg
     1621 caataaatac ggcaagtagg gtatcagttc cgtagattcc gctgtcaaag acttggccag
     1681 tttttcttca accgttgaca taatacactc tcctttttat acccaattat gcattctgct
     1741 atttttcagc tacaacaagc atttcaaaat cctcagttga aagcttatcc gctctcgaat
     1801 atgatgatac aaccctgtat gacggttatt tgtctatata tttgcccaaa aaatccgcaa
     1861 aatcttcgct cacttcatat cttgaaatga aatcgatgtt tatataatac ggagaatcga
     1921 aatatacacc ctttttatta tatatcctga ttgttacaag attctccaac gccgcaccgg
     1981 cagaaccctg accggcatgc gacactccag acccggtatt gcccatatcc atcataccat
     2041 cggatgtatt agactgcgaa acctcgtttg ctatgggtgt atcacctgat actgcaccta
     2101 cttcaccctt ttcaggcatt tccagcggca agtcctctgc aacgccggtc ccgtccgatg
     2161 cctgatccat tggccggtgc tcactgcggt caatttggtc cggtgctgtc atatccggct
     2221 caaccgcatc aggagggtct atcctgtccg gctgcacatt atcaatttcg atgcctttac
     2281 cggaatccgt atttctgtaa ttgaatatgc tgtcgaaata tttatcgctg gaattcttca
     2341 atacggaata aaaactatag aaccgttcaa tctcatccct gtccgttatt tctcctaaaa
     2401 tatccgtccg accttgcagt ttgctttgct ccgagtggcc tatgaactga atttttgcta
     2461 tatcatcagc cctgtcaatg ccaaacagag ccagatatct gactgaatct tcatcctgat
     2521 tgttattata gctttcaaaa gtaaagaacc ggaaaagttg atattcatcg tcttttttta
     2581 ccgcaaccac tgattctccg ccggcgggaa tatagctgta tacttcattt cctatcagac
     2641 ttttatcggg accggctgtt atatctgcga ttttaccgcc gatatcactt tctatgacag
     2701 cagttggcag accatactcg gccctcaatt cgtcggataa tattatataa tgtctgtcat
     2761 caatctgaaa ctggttcaaa agcggggcaa ttgcgtcctc ccttcccatg ctgacatcat
     2821 tttctacacc atatgtcaga gatgaaccac gatcatgttt cttcgacata tggttgtaag
     2881 tcagcaagcc tcctactatc acagccgtta aaaccaatgc aggaatggct tttttaaaat
     2941 taaacaatgt cacaaagact cccttccttc tatccgaatt attcaaaaca ttatccagca
     3001 tcctcttttt tgcagattca cttggcttaa tccgatcaaa agaattctta aaatcctctt
     3061 ttttcactca aaatcacctt ccatttccat tttcaaaagc tttctgcctg tgtggagata
     3121 gcctctaact gtggatggat ccttttccaa tatttttgct atttctacag tagaatagcc
     3181 ttcataataa tataagtata tggcggcctt gtatttggcg ggaagttcca ttaccttttt
     3241 aagaacaccg tctatattaa ttggctgctc gacagcttct tcttccacat gattgaagcc
     3301 cactgttttc gaccaccaat tcttaaaatg atctttgcat agatttgtag ctgtcctgat
     3361 tagccatgcc ttctcatgct cttcgctttg aaaaaccgtt ttgtccttca taagccggat
     3421 aaacgtgttt tgaaccatat cctccgtatc ggacctgttt ttcataaaca tgaaacaaat
     3481 gcggtagacg gtatttacat ggcgttcata aatctctaac agctcttcat tcgtacgcaa
     3541 taaagaactc tgcaattgac ttcccccttt cactattaat acgattgaaa acgcaaaaac
     3601 gttttgtaaa ttttcatgta aaatatgggg ttataagtga cctgttgcat cctttcgcaa
     3661 atctaatcgg atacctttta gaactgtgtt tataaatttg cccatttcca taatgtattg
     3721 caaactttat gaaatctttc ccgaagtata ggtatcagaa atccaaaggg gacaaacata
     3781 agtataattt ctgttcagat cccaatataa agtacttctc aaaaaaatat atactcataa
     3841 tcaacaccct tttatccttt gtttatatgc ctgattatat atcatattat tgctgatatc
     3901 aaagtatttt cttcccacgg ctaacggatg aatatagggg gtttagcgtt tttattcatc
     3961 gataccgtta tatacagcta ttgcatcggt aatattatat tgttccgaat gctgattccc
     4021 ttttgcaccg gctgtaatca gaatatactc atgtttgccg actttagcca gactggcaag
     4081 gcataggcct gccttatcgg tatatccggt tttacctcct aaaatttccc catcgataat
     4141 attttgattt ttaaatttga gtttttcaaa catggtactg taataggtta ttccgtcagg
     4201 gtgtatattc gtaggtcgcg ttgaatgacg ggacgaagta aagatctccc tgaatatttc
     4261 attttgtaaa gcatagctta aaagaacaga cagatccttg actgttgtgt aatggttttc
     4321 gtcgtgaagt ccagttgaat tttcaaaatg agtattattc atgccaagct cttttgcctt
     4381 ctgattcatc atctctacaa aatttcgttc cgaacccgca attcgttctg caagcccgat
     4441 acagcactct gcaccgcttg gaagtattgc cccgtacaaa aggtcaatca ctcttaccct
     4501 ctcacccggt tggaagcctg ccattgatgc gtctgcttca tacagcccct gaaacataga
     4561 atcggtaagt tttatttctt cctttagatc cggtaaattt tctatcgcaa cgatagctgt
     4621 catgattttg gtcaaagaag cagggtaaat tctttcttcg ctattttttt gcatcaggac
     4681 ggcacaatca tctaaacgga taagaatcgc attagaactg ctgagtttat cggaagatat
     4741 tgaaacagaa ggtttgggta ttgtatccga tgataaattc ggcaagattt tattaccata
     4801 ctcttcctca agtatattac tattttccgg aataacgata tatttgcaaa caaaaaaagt
     4861 aattccggct atcaacaaaa aagttataaa tatacttttc ctcgactttt tctttttaaa
     4921 tttttgtacc atatcaaaca tctcctaact ctgtgataat atcatttaac cacagaatga
     4981 ggagccatat cggaatttta tcttatgaac ttcttaagat tttctgactt atgaataaaa
     5041 agttatttct ttataaatta ttttttatat tggaagcttt actgtaaatg tagttttttc
     5101 cgcattgctt tccacagaaa tttccccgtc atgagccgtg acaatttcac gggcaattgc
     5161 caatcccagc ccagctccgc cggtgtttgt ggaacgagcc gaatccaacc ggtaaaactt
     5221 ttcaaaaatg gtttcaagct tttgagaagg gatgggatcc ccctgatttg tgaaagtaac
     5281 aatgacttta tcctcctgcc tgtctgccgt aatatcaata atgctgttct cataactata
     5341 ggaaattgca tttttcaata tattattgaa aacacgggca agcttgtctc catcccctgc
     5401 tataaccaga tcctccgggg cattgatggt aatctgtttt ctaaaaggtg caagcatggg
     5461 ataaaactcg tcagccagct gttgaagcat aaatgcgagc tttatattgc ttttattcaa
     5521 tacaatggac tgcaggttga atcttgtaat ttcaaaaaac tcattaatca gctcttccaa
     5581 ccggtatgct ttctctaatg ctataacggt atatttcgat ctttgctctt tcggcatatc
     5641 cgatgcttca tctaaaagac tcaaatatcc gattactgaa gcaagcggtg ttttcaagtc
     5701 atgtgccaga taagtaatca gatcgttctt tcgctgcatt tccatttgca taagctgctg
     5761 atgcttttgt gcaaccgatt ttatttttag aagctgtctt tcaatttcgg aatattcttt
     5821 tggtaatgtt aagtcatcag tacctgatgt catgaaagag tccataacac cggtaataaa
     5881 agatatctgc ttttttcttt tataacgtga atataaataa gttgaaaggc ccatacatag
     5941 tgctaaaaat gcaataaatg cgacaaatgc atagatgata aattgtttta tttcatacca
     6001 acttttgaat attatcgcta ttttcccttg acctaaatgt tccggttcaa taataaattt
     6061 attgtatatc cactctttaa ttattccgtt aaacccttca tccaccaacc gtactgtgcc
     6121 cagacaaaac agaataactg cggatataat aaaaaaaact tttagtaagc tattaacatt
     6181 atttttcaat tttatatccg accccccata ccgttttaat atatttggga tgttctttgc
     6241 tgtcgttcat tttctctctt aaatgtcgaa tatgaaccat tactgtatta ttgctattgc
     6301 taaaatattt gtccccccag acttcacgga ataattcttc tgaactgact acgtgccctc
     6361 ggttggaaca tagcacccat aaaatagcaa actccgtagg agtaagtgca agctttcttt
     6421 cgtttaatgt acattcatga gtatccttat tgataactaa accggcaaat tgaattatat
     6481 tttcctcctg tagcaattcg gcagaattgt atttagtaaa ccttcggagc tgtgccttga
     6541 cacgggcaac aagctccaaa ggccgaaacg gtttcgtcac ataatcatct gcccccaggg
     6601 tcaaaccggt aattttatcg atttcttctt cttttgcagt cagcataata acaggaaaat
     6661 tgtgcttttc cctgattttc cggcagaggg taaaaccatc aatatccggc agcataacat
     6721 caagtattgc aagctctaac tgatttgact caacatatcg caacgcatct ttaccgttat
     6781 aagatttgta tactgtaaaa ccttcattat ttaagtaaac ttcaatcaga tccgctatgg
     6841 actgctcatc atctaccact aaga
//
LOCUS       DAOOJC010000075         6842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_9226_length_6842_cov_3.855459, whole genome shotgun sequence.
ACCESSION   DAOOJC010000075 DAOOJC010000000
VERSION     DAOOJC010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6842
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9226_length_6842_cov_3.855459"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1161
                     /locus_tag="PLA01_03310"
     CDS             136..1161
                     /locus_tag="PLA01_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HPD00360.1"
                     /translation="MKKENCVTINSQFFNSYSNGTMKYLPVFLTEEDIKSLNYPEILE
                     EILMKADRLTLNYPEVLDEILRKVDASVFSSKSGSVLSALKSYSQTLLENNSINLKTV
                     ATGSAMALLLIYASKGLDNKSQLDIKNSLINSMGYSQLTSNSTSFQEGSFQEVEEMIY
                     ETVKSAPLPNTLVCSAEIPFDVNIKKPAYDMPVLASRGNSKPEVISGTALSELKKMDS
                     SELIKQSKKKFHMTATAYDLCYKSCRKTREHPAYGITASGARATVGRTIAVDPKVIPL
                     GSKVYIVFPEKYSHLNGVYIAEDTGSLIKGNKIDVFFGEDNPGETVVNKKAYEFGLQL
                     VEVYLLE"
     gene            complement(1168..3147)
                     /gene="uvrB"
                     /locus_tag="PLA01_03315"
     CDS             complement(1168..3147)
                     /gene="uvrB"
                     /locus_tag="PLA01_03315"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPD00361.1"
                     /translation="MHKFKLVSDYKPCGDQPEAVDRLVEGINIGFRAQTLLGVTGSGK
                     TFTMANVIERVQKPTLVIAHNKTLAAQLCSEFKEFFPNNCVEYFVSYYDYYQPEAYIP
                     STDTYIEKDSSINDEIDKLRHSATAALFERRDVIIVASVSCIYGLGDPEDYTDLMLSL
                     RPGMTRERDDIIRKLVDIQYERNDIDFRRGKFRARGDILEIFPASSSDKVIRVEFFGD
                     EIERITEIDSLTGEITGVMSHAAIFPASHYATTRAKMDRAIESIEQELEERLKEFRSE
                     GKLLEAQRLEQRTRYDIEMMQEVGFCQGIENYSRHISGRKPGSAPYTLIDYFPDDYLL
                     IIDESHVTVPQIGAMYNGDRSRKESLVDYGFRLPSAFDNRPLKFEEFEERINQIVFVS
                     ATPAKYEKQHSKQIVEQIIRPTGLLDPEVVVKPVKGQIDDLIGEINQRVESKQRVLVT
                     TLTKKMAEDLTDYLNELDIKVKYLHSDIDTIERMEIIRDLRLGVFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSETSLIQTIGRAARNVEGKVIMYADNVTDSMKKAIDET
                     NRRRGIQSEYNRKHGITPKSIEKGIRDVIEVSKIAEEDAKYFIRGDKESMSKEQVQSI
                     IDKLEKEMKHAAAQLQFERAAELRDKISELKKKTD"
     gene            complement(3190..5373)
                     /locus_tag="PLA01_03320"
     CDS             complement(3190..5373)
                     /locus_tag="PLA01_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HPD00362.1"
                     /translation="MPSNNLVEQNIVDTLEKNYMPYAMSVIVSRAIPEIDGLKPSHRK
                     LLYTMYKMGLLNGEKTKSANVVGQTMKLNPHGDMAIYETLVRLTRGNNALLHPYVDSK
                     GNFGKQYSRDMKFAAPRYTEVKLDKICEELFRDIDKNTVEFIDNYDGTLKEPVLLPCT
                     FPSILVNANQGIAVGMASNICSFNLVEVCKTAIEYIKNEDTDVLEHLKAPDFPTGGQL
                     IFNEKDMREIYDTGRGSFKVRAKYRYDRTNNCIEIYEIPYKTSSEAIIDAVIELVKSN
                     RIKDVVDVRDETDLNGLKITLDIKKNSDPDALMNKLYKLTPLQDSFSCNFNILIEGRP
                     RVMGIKEVLKEWIIFRVDCIKKQTLFDIEKKSDKLHLLLGLKKILLDIDKAVSIIRNT
                     EEEALVVPNLMKGFDIDQVQADFIAEIKLRNLNKEYILNRISEIENLQKEIADLRDLH
                     SSDKRIKKVIIKQLEDVSKKFGQSRRTEIIHEEHVEEITSDHLIEDYNLKLFLTKHNY
                     LKKIPLTSLRSSSEHKLKDDDCIVQEVETHNKAELLLFSNKQTVYKLKIFEVEDCKAS
                     SLGEYLANLLELENGEEIIYMAATDDCKGYMIFAYENGKVAKIDMGSYATKTNRRKLS
                     NAYCGLSPVVYMDYIIEDEELVAFSSLNKVLIFNTDSINSKSTRDSQGVQVLKAKKGS
                     IMNRIKKINEVSFKDPDYYRTKNIPAIGCYLKNEDIEDGQVKLEL"
     gene            complement(5417..>6842)
                     /locus_tag="PLA01_03325"
     CDS             complement(5417..>6842)
                     /locus_tag="PLA01_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HPD00363.1"
                     /translation="LFVSSFPASTLTLKKQAVVNAGLKFVLNDEESGQTFEYCYENGI
                     VDYIKEISGDKGFTDIQFYETATRGRDREDKPEYKVKMQIAFCFNNEINLLEYYHNSS
                     FLEHGGAPDKAVKNAFVYEIDRCLKSEGKYNKDESRITFQDIQDSLILVTNSFSTITS
                     YENQTKKSITNKFIQEAMTDFLKQQLEVYFIENKMESNKILEQVLVNKRSRETAEKTR
                     INIKKKLGGSIDISNKVKKFVDCRSKDVNKREIYIVEGDSALGACKLGRDAEFQAIIP
                     VRGKILNCLKAGYDGIFKSDIIVDLLKVLGCGVEIKSKHIKDLNTFDLNNLRWNKIII
                     CTDADVDGFQIRTLILAMIYRLVPTLIHAGKVYIAESPLFEIRAKDKTYFAYSDKEKN
                     EIVSKLKGKYAILRSKGLGENEPDMMWQTTMNPETRRLIKVLPDDAEKTKEFFDLLLG
                     DNLAGRKSYIEENGHKYLDMIDVS"
BASE COUNT         1884 a         1588 c         1150 g         2220 t
ORIGIN      
        1 ttttagcata attttcattt atcgtataat ttgttcttta aaaggaagaa aatttttaat
       61 ataatagata atacaatata tatgtgtctc tcttttttag tcatcaaaac gtgcctgtcg
      121 ttggggggta atattatgaa aaaagaaaac tgtgtaacga taaactctca gttctttaat
      181 agttattcga acggtactat gaaatatctg ccggttttct taacagaaga agatataaag
      241 agtttgaact atcctgagat tcttgaagaa atccttatga aagcggaccg tttgacatta
      301 aattacccgg aagtgcttga cgaaatattg aggaaagttg atgcttctgt cttttcttca
      361 aagtcaggtt ctgtcctgtc ggcattaaaa tcttacagtc aaactttatt agagaataac
      421 agcattaatc tcaagactgt tgcaacagga tctgccatgg cacttctgct catttatgcc
      481 agcaaaggtc tggacaacaa atctcaactc gacataaaaa acagtttgat taattccatg
      541 ggatattctc aattaacgtc aaacagtaca agctttcaag aaggtagttt tcaagaagtt
      601 gaagaaatga tatatgaaac agtaaaaagt gcccctctac cgaatacttt ggtttgttct
      661 gctgaaattc cttttgatgt gaatattaaa aagccggcat atgatatgcc tgtgctggca
      721 tcgcgaggca attcaaagcc tgaagtcata agcggtacag ccctttctga attgaagaaa
      781 atggattctt cagaattgat aaaacaaagc aaaaagaaat ttcacatgac agccactgca
      841 tacgatttat gctataaaag ctgccgcaaa acaagagaac atcccgcata cggaattacc
      901 gcctcaggag cccgtgccac agtgggaaga accattgccg ttgatcctaa agtaatccca
      961 ttgggaagca aggtatacat agtcttcccg gaaaaataca gccatttgaa tggagtatac
     1021 attgcggaag acaccggaag tctcatcaag ggtaataaaa tcgatgtctt tttcggagaa
     1081 gacaatcccg gagaaacggt tgtcaacaag aaagcttatg agttcggact gcagctggtt
     1141 gaagtctatc ttttggagta acgcaaacta atccgttttc ttttttaatt cactgatttt
     1201 atctctcagc tctgctgctc tttcgaactg gagctgggct gcagcatgtt tcatttcctt
     1261 ctcaagcttg tcgataatgc tctgaacctg ttccttgctc atggattctt tatcaccccg
     1321 gataaagtat ttggcatcct cttctgcgat cttagaaact tcaattacat ccctgatgcc
     1381 cttttcaatg ctttttggag taattccatg cttccggtta tactccgatt gtatccctct
     1441 tcttctgtta gtttcgtcaa tagccttttt cattgagtcg gtcacattat ccgcatacat
     1501 aataaccttg ccctcaacat ttctggccgc tcttcctatg gtctgaatca atgaagtttc
     1561 cgaacgcaga aagccttcct tatcggcatc caatattgcc acaagggaaa cctcgggcaa
     1621 atccaagcct tccctcaaaa gattaatccc tacgagaaca tcgaaaaccc caagcctcaa
     1681 atcacggatt atttccattc tttcaattgt gtcaatatcc gaatgaagat attttacctt
     1741 tatatccaac tcattcaagt agtctgtaag gtcctctgcc atcttctttg taagggtggt
     1801 aaccagtaca cgctgtttac tctcaacccg ctggtttatt tccccgataa ggtcatcaat
     1861 ctgcccctta accggcttta ctaccacctc aggatccaaa agtccggtag gccttattat
     1921 ctgctctact atttgcttcg aatgctgttt ttcatactta gccggtgttg cacttacaaa
     1981 cacaatctgg tttattctct cttcaaactc ctcaaactta aggggtctgt tatcgaaagc
     2041 cgaaggaagc ctgaaaccat aatctaccaa tgactccttt cttgatctgt ctccgttata
     2101 catggcaccg atctggggaa ccgttacatg ggattcatca attatcaaca aatagtcatc
     2161 gggaaaataa tcaataaggg tataaggagc acttccgggt tttcttccgc taatatgcct
     2221 cgagtagttt tctatcccct gacaaaaacc aacctcctgc atcatttcaa tatcatacct
     2281 tgtcctctgt tcaagtcttt gtgcctctaa aagcttgcct tcggatctaa actccttcag
     2341 gcgttcctcc aattcctgct ctatagattc tatagccctg tccattttag cccgggttgt
     2401 ggcatagtga gatgcaggaa atatagcagc atgggacata accccggtaa tttctcctgt
     2461 aagggaatca atctctgtaa tcctctcaat ctcatcacca aaaaactcta cacgaataac
     2521 cttatcggaa gaagacgccg gaaatatctc aagaatatct ccccttgccc tgaattttcc
     2581 tcttctaaaa tcaatatcat tacgctcata ctgaatatcc accagcttcc ttatgatatc
     2641 atccctctcc cttgtcatac ccggccttaa agaaagcata agatcagtgt agtcctcggg
     2701 gtcacccaat ccgtatatac acgacacgct ggcaactatt attacatccc ttctctcaaa
     2761 caaagcagcg gtagcagaat gtctcagttt atcaatctca tcgtttatgg aagaatcctt
     2821 ctcaatatag gtatccgtgg atggaatata agcctcgggc tgataataat cataatagct
     2881 gacaaaatac tcaacacagt tattcgggaa aaattccttg aattcgctgc atagctgagc
     2941 tgccagggtt ttattatgtg ctattacaag ggtaggcttc tgaaccctct ctatgacatt
     3001 cgccatggta aaggttttac ccgatcctgt aacacctaga agggtttgtg ccctaaatcc
     3061 tatatttatt ccctcaacca gtctgtccac cgcttcaggc tggtcgccgc aaggcttgta
     3121 atcggataca agcttaaact tgtgcataaa cacacctcaa tcccgatttc ttttttaaga
     3181 actcacaatt tataactcaa gctttacctg cccgtcctca atgtcttcat ttttcagata
     3241 gcagcctatt gcaggaatat tctttgttct gtaataatca ggatctttaa aggaaacctc
     3301 attaatcttt tttatccggt tcattatgct gccttttttc gccttcaaaa cctgaactcc
     3361 ctgagaatcc ctggtggact ttgaatttat actgtcggta ttgaaaatca aaaccttgtt
     3421 caagctgcta aaagcaacca attcttcatc ttcaataatg tagtccatat atacaaccgg
     3481 agataatccg caataagcgt tggaaagctt tcttctgttt gtcttggtag cataactgcc
     3541 catatctatc tttgccacct taccgttttc ataggcaaaa atcatatagc ccttgcaatc
     3601 gtcggttgca gccatatata ttatttcttc gccgttttcc agttccagca gattagccag
     3661 atactctcca agactgcttg ccttacagtc ttcaacctca aatatcttaa gcttgtacac
     3721 cgtctgcttg tttgaaaaca gcagcagttc agccttgtta tgggtttcga cttcctgcac
     3781 gatacagtcg tcatccttga gcttgtgctc agaactgctc cttaatgacg tcaaaggaat
     3841 ctttttcagg tagttatgct tggtaaggaa cagcttcaga ttataatcct ctatcaggtg
     3901 gtcactggtt atttcctcaa catgctcttc atgaataatc tcagtacgcc gggattgccc
     3961 aaatttcttg gacacatctt ccagttgttt tataataact tttttaattc ttttgtcact
     4021 gctgtgcaaa tccctaagat ctgcaatttc cttctgaagg ttttcgatct cgctgatccg
     4081 gttcaaaata tattccttgt taagattcct cagctttatc tctgcaataa aatcagcctg
     4141 cacctgatca atgtcgaacc ccttcataag gttaggaacc accagggcct cctcctcagt
     4201 atttctgata atgcttactg ctttgtcaat atcaagaagt atctttttca atcccagtaa
     4261 aagatgaagc ttatccgatt ttttttctat atcaaacagt gtctgttttt ttatgcagtc
     4321 taccctgaaa atgatccatt cctttaagac ttccttaatc cccattactc tagggcggcc
     4381 ttcaatcaat atgttaaagt tgcagctaaa gctgtcctgt aatggcgtaa gtttataaag
     4441 cttattcatt aatgcatcag ggtcactgtt cttcttaata tccagtgtta ttttaagacc
     4501 gtttaaatcc gtctcatctc tgacatctac aacatcctta atcctgttgc tcttcacaag
     4561 ctctatgact gcatcaataa tagcctctga agatgttttg taaggtatct catatatctc
     4621 aatacaatta ttagtcctgt catatctata ttttgccctc accttaaagc ttcccctgcc
     4681 ggtatcatat atttcccgca tgtccttttc attgaatatc agttgtcctc cggtgggaaa
     4741 atccggtgcc ttcaagtgct caagaacatc cgtatcctca tttttaatat actcaattgc
     4801 agttttgcaa acctcgacaa gattgaaact gcaaatatta ctagccatac caacggcaat
     4861 accctggttc gcattgacca gaatactggg aaaggtgcat ggcaaaagaa ccggctcttt
     4921 caatgtcccg tcatagttgt ctataaactc cacggtattc ttgtctatgt ccctgaaaag
     4981 ctcctcacat attttatcca gctttacttc agtataacgg ggtgcagcaa acttcatgtc
     5041 ccttgaatac tgtttcccga aatttccctt cgagtccaca taaggatgta aaagtgcgtt
     5101 attcccgcgg gtcaatctca ccaatgtctc gtaaatggcc atatctccat ggggatttag
     5161 cttcatggtt tgtcccacta catttgcaga tttggtcttt tctccgttta gcaatcccat
     5221 tttgtacatg gtataaagaa gctttctgtg tgaaggcttg agtccgtcta tttctggtat
     5281 tgctcttgac acaatcacac tcatggcata gggcatatag ttcttttcaa gagtatcaac
     5341 tatattctgc tcaaccaaat tattcgatgg catatcatta cttcaccttt ctgtttgtct
     5401 aaatttcaat aatccatcag cttacatcta tcatatcaag atatttatga ccgttttcct
     5461 caatataaga tttccttccc gcaaggttat ctccaagaag aagatcgaaa aactctttgg
     5521 ttttctcagc atcatcagga agcaccttta ttagccttct ggtctcggga ttcatcgtag
     5581 tctgccacat catatccggt tcgttttctc caagtccttt ggaccgcagt atggcatact
     5641 tgcccttcaa tttggaaaca atttcgttct tttccttgtc ggaataagca aaatacgtct
     5701 tatcctttgc cctgatttca aaaagaggtg attccgcgat gtacaccttt cctgcatgta
     5761 taagtgtagg aaccagtctg tatatcattg caagtatcaa ggtcctgatc tggaatccat
     5821 ccacatctgc atcggtgcaa attataattt tgttccaccg aagattgttt aaatcaaagg
     5881 tgttgagatc tttaatgtgc ttgctcttaa tctctacacc gcaccctaaa acctttagca
     5941 aatcaacaat tatatcactc ttaaaaatac cgtcataacc tgctttaaga cagtttaaaa
     6001 tcttgccgcg aacaggtatt atcgcttgaa actccgcatc tctgccaagc ttacaagcac
     6061 caagggccga atccccttcc actatataaa tctctctctt gttgacatcc ttgctcctgc
     6121 agtccacaaa tttctttact ttatttgaaa tgtcaatact gccgccaagc tttttcttta
     6181 tattaatcct ggttttttcc gccgtttccc tgctcctttt atttaccaga acctgctcta
     6241 aaatcttatt gctttccatt ttgttctcaa tgaaatagac ctccaactgc tgtttcaaga
     6301 aatcggtcat tgcttcctga ataaacttat tggttatgga ctttttggtc tggttctcgt
     6361 agctggtaat ggtagagaag gagttagtaa caaggataag gctgtcttga atatcctgga
     6421 atgttattct tgactcatcc ttattgtatt tgccttcaga tttaaggcac cggtctattt
     6481 catagacaaa agcatttttt actgccttgt ctggagctcc tccatgttcc aaaaagcttg
     6541 agttgtgata gtattcaagc aaattaatct cgttattaaa gcaaaaagct atttgcattt
     6601 ttaccttgta ttccggcttg tcttccctgt cccttccccg ggtagccgtt tcatagaact
     6661 gaatatcggt aaaaccctta tccccggaaa tctcttttat atagtcgact ataccgtttt
     6721 catagcaata ttcaaaagtc tgtcccgatt cttcgtcatt gagaacaaac ttcaatcctg
     6781 cgttaacaac agcctgtttc ttaagcgtaa gcgtggatgc gggaaaactg gaaacaaata
     6841 aa
//
LOCUS       DAOOJC010000076         6840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_9230_length_6840_cov_3.746500, whole genome shotgun sequence.
ACCESSION   DAOOJC010000076 DAOOJC010000000
VERSION     DAOOJC010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9230_length_6840_cov_3.746500"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PLA01_03330"
     CDS             complement(<1..824)
                     /locus_tag="PLA01_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPD00364.1"
                     /translation="MRKSVSGALVVIMLLAMLTPINGLFAIAGTEQILFINEVMSSNE
                     STIRDGDVDDSKFGSQGGAYSDWIEIYNAGAQDVDLTGYTIADSSAVWTFPQGIVPAG
                     GYLLIWASDKNKVAADGQLHTNFKLSASGEPLIFKAPDGTVVDSVTVIGLADDQSYGR
                     ENDGSSKFVAFTRATPGSANIPEISTEQVLFINEVMASNVTTLRDGDVDDPEFGKQGG
                     AYSDWIEIYNAGDRDVNLAGYIIADTSAEWVFPNVTVSAGGHLLIWASDKNKVASDG"
     gene            complement(1501..4032)
                     /locus_tag="PLA01_03335"
     CDS             complement(1501..4032)
                     /locus_tag="PLA01_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPD00365.1"
                     /translation="MKIGFSTLGCPDFSWPEIYSMAKDFGFDGIEIRGLGNEIFAVKA
                     KPFTESQLSHTAKKLAELRLEIPCFSSGCCLKFAENAQKNHEEIVEYITLASRLGTPY
                     VRILADLEPQPCGDVDDKVVLEALKMLTPIAEEKGVTLLVETNGVYSDTSRLCRLLDS
                     VASDAVAALWDVHHPYRFAGESPGKTVQNLGAYIKYVHIKDSVVENGVIRYRMMGEGD
                     MPIDDIMLALRSINYDGYITLEWVKRWAADLDDAGVVFPNFANYMSRYTKKNVVKGRL
                     FDNAAGTGKYIWEKDTLIDLTFPQVLDRLVEEFPDQYAFRYTTMDYTRTYSEFRDDVD
                     TFARSLIALGVKPGDHVAIWATNVPQWFITFWAATKIGAVLVTVNTAYKVYEAEYLLR
                     QSDTHTLVMIDGYKDSDYVGIIKELCPELETAEAGKPLHIKRLPFLRNIITVDSRQKG
                     CLTWDEAIAMADRVPVEEVYRRAFSINKHDVCNMQYTSGTTGFPKGVMLTHYNVINNG
                     KCIGDCMDLSTADRMLIQVPMFHCFGMVLSMTASMTHGSTMCPIPYFSPGVSLDCINS
                     EKITAVNGVPTMFIAMMEHEKFKDTDFSHMRTGIMAGSPCPIKVMQDVVEKMNMTQIT
                     IVFGQTEASPGCTQSRVDDPIEVRVNTVGRPLPGIECKIVDPVSGEDLPDNTDGEFVA
                     RGYNIMKGYYKMPEATASAIDENGWLHTGDMARRDENGNYKITGRIKDMIIRGGENIY
                     PKEIEDFIYTHPKVKDVQVMGVPDKQYGEEIMACVILKDGETMTADELKDYVRSHMAK
                     HKTPRYVDFVNEFPMNAAGKILKYKMRERAVEKLSLQEANSVVTA"
     misc_binding    complement(4202..4466)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4537..4926)
                     /locus_tag="PLA01_03340"
                     /pseudo
     CDS             complement(4537..4926)
                     /locus_tag="PLA01_03340"
                     /inference="COORDINATES: protein motif:HMM:NF022443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
     gene            complement(4984..6528)
                     /gene="cls"
                     /locus_tag="PLA01_03345"
     CDS             complement(4984..6528)
                     /gene="cls"
                     /locus_tag="PLA01_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPD00366.1"
                     /translation="MWHKIKTIIRFFYHRVVLISLAIVIQLAVLIAVIIRFNNYFVLF
                     YWGSIFVSLFTVFWILNDKSNPSYKIAWIIPILLFPIFGGLFYIFFGGKKLSKREKKR
                     LMSIGLKIQNALVPQPHVLEKMMPHDTGAVAQSRYIQNIAFFPPYQNTTGEYFPKGEL
                     KFERLKEELKKAEHFIFMEYFIIKEGVMWNSILDILTEKAKKGVDVRIIYDDLGCLLL
                     LPRDYYHKLEHLGIKCCVFNPVRPVLSAKINNRDHRKITIIDGHTGFTGGINLADEYI
                     NKIERHGYWKDNAIMIKGEAVWNMTVMFLSMWDYLKGVGEDFTRFKNNNPFKTESTQG
                     YFQPFADSPFDDETVGEIVYLNLLNKSNKYVYITTPYLVIDYGMTKALTAAAKSGVDV
                     RIITPYLADKWYVHAVTRSNYRALIENGVKIYEYTPGFIHSKTLVSDDEYAVIGSINM
                     DYRSFYLHFECGVWMYKSGSVLAVKEDFLDTLKACKEITMKDIKEMKWYRRLIGTVLR
                     IFAPLM"
BASE COUNT         2113 a         1556 c         1097 g         2074 t
ORIGIN      
        1 ccgtctgatg ctaccttgtt tttatcggag gcccagatta aaagatgtcc tccggcagat
       61 acagttacat tcggaaaaac ccattccgct gaagtgtcgg ctatgatata accagccaga
      121 ttgacatccc tgtcaccggc attatatatt tcaatccaat cggagtatgc ccctccctgt
      181 tttccaaact cgggatcgtc cacatcgccg tctcttaagg tagtaacatt ggatgccata
      241 acctcattaa taaacagtac ctgttctgtt gaaatttccg gaatgtttgc tgaaccggga
      301 gtagccctgg taaaagcaac aaatttcgat gaaccgtcat tttcccgccc atagctctgg
      361 tcatcggcaa ggccaataac tgttaccgaa tcaacaacag taccatcggg agccttaaaa
      421 atcaatggtt cgccggatgc actcaacttg aaattggtat gcagttgtcc gtctgctgcc
      481 actttgttct tgtccgaagc ccaaatcagc aggtaacctc ctgcaggaac aattccctgc
      541 ggaaaagtcc atacagccga agaatcagca atagtatagc cggttagatc tacatcctgt
      601 gcaccggcat tgtagatttc aatccagtcg gaataagccc ctccctggct tccgaacttg
      661 gaatcatcca catcaccgtc ccgaatagta gattcattgg aagacatgac ctcatttata
      721 aagagaatct gttctgtccc cgcaatggca aaaagcccat taataggtgt caacattgcc
      781 aataacatta tgacaacaag ggcacccgat acggattttc tcatcctcat ctccccttct
      841 ttaaatattt atgtattata aaaaataata ctcctgtatt ggttcaaaat aatactgcac
      901 atatattagt tcgtttttta ctaaattctt taggcgaatt ttaaggattt tctctaacga
      961 atttaaagaa ttgcacctga ataatactaa gaatgatgag tttatgttaa tctgtttaac
     1021 atgcaaagaa taaatttcta atcaataaaa acatccttaa aacatactaa aatacatcct
     1081 cgaaacatac ttacccttaa taactatttt ataactaaaa cattaaataa tcaataatgc
     1141 aataaaacat ggctgcatta aattaatgtg aggataaaca gtattcatca tattttatta
     1201 ttacatataa gaatatagta aatggaaatt gctatatgtg tgaataagcg gttgagtaat
     1261 aataaagtca ttaggagggc aggctgggaa aataaattta ataataagct gcataatcaa
     1321 atttaaggtg atacagcatg ggtgcattaa ataaaatatg cacccataca gcatctttct
     1381 aacctccgga cttcaatgag atgatatttt ctcaataact gcaatctata tccaaattgt
     1441 ccgtttactt ctctgttcca taactatgcc gggaacaatc agcttcaata taaccgggca
     1501 ctatgccgta acgacagagt ttgcctcttg caaagacaat ttttcaacag cccgctccct
     1561 cattttatat tttaatattt ttccggcggc attcatcgga aactcattaa caaaatcaac
     1621 ataccgtgga gttttgtgtt ttgccatatg ggaacggaca taatccttaa gctcatcggc
     1681 agtcatcgtt tcaccgtctt ttaaaattac acatgccatg atttcttcgc catactgttt
     1741 gtcgggaact cccataacct gtacatcctt aaccttcgga tgggtgtaga taaaatcctc
     1801 aatctccttc ggataaatat tctccccacc gcgaataatc atatccttga tgcggccggt
     1861 tattttatag tttccatttt catcgcgtct tgccatatca ccggtgtgaa gccatccatt
     1921 ttcgtcaatg gcagaagctg tggcttccgg cattttatag tatcccttca taatattata
     1981 tccgcgggca acaaactctc catcggtatt atccggcaaa tcctcaccgg ataccgggtc
     2041 aacaatcttg cattctatac ccggaagcgg acgtccgaca gtatttacac ggacttcaat
     2101 gggatcatcc acacgactct gcgtgcatcc gggcgatgcc tcagtctggc cgaaaacaat
     2161 ggtaatttga gtcatattca tcttttctac aacatcctgc atcactttaa tcgggcatgg
     2221 acttccagcc ataataccgg ttctcatatg agagaaatca gtgtctttaa atttctcatg
     2281 ctccatcata gcaataaaca tagttggaac cccattcacg gcagttattt tctcgctgtt
     2341 gatacaatcc aaagacactc ccggtgaaaa ataaggtatc ggacacattg tcgagccatg
     2401 ggtcatagat gcagtcatag acaataccat accaaagcag tggaacatcg gcacctgaat
     2461 cagcatacgg tcggctgtag aaagatccat gcaatccccg atacatttcc cgttgtttat
     2521 tacattgtaa tgagtaagca taactccctt cggaaaacct gtagtgcctg aagtgtactg
     2581 catattacaa acatcatgct tgtttatgga aaaggcacgg cggtatactt cctcaacagg
     2641 aaccctgtct gccatcgcaa ttgcctcatc ccatgtcaag catccctttt gccttgaatc
     2701 aacagttata atattgcgaa ggaagggcaa acgctttata tgcaagggtt ttccagcctc
     2761 tgccgtctcc agctccgggc aaagttcctt gataataccg acataatccg aatccttata
     2821 tccatctatc ataaccagcg tatgcgtatc cgactggcga agcagatatt cagcctcata
     2881 tactttataa gcagtgttga cagtgacaag cactgcaccg attttagtag ccgcccagaa
     2941 ggtaataaac cattggggaa cattggtagc ccaaatggcg acatgatctc ccggtttaac
     3001 tcccaatgca ataagagacc gggcaaaagt atcaacatca tctctgaact ctgaatatgt
     3061 ccgggtataa tccattgtag tgtatctaaa cgcatattga tccggaaact cttcaacaag
     3121 acggtcaagt acctgaggga atgtaagatc aatcagggta tccttctccc atatatattt
     3181 tcccgtaccg gcagcattgt caaacaaacg tcccttcaca acattcttct tagtataacg
     3241 gctcatatag tttgcaaagt tcgggaacac aacaccggca tcgtcaaggt cagccgccca
     3301 tctctttacc cattcaagag taatatatcc atcgtagtta attgacctta aagccagcat
     3361 aatatcatca atcggcatgt ctccttcacc catcatgcga tagcgtatca cgccattttc
     3421 caccacagaa tccttaatgt gaacgtattt aatatatgca ccgagattct gcaccgtctt
     3481 tccgggcgac tcacccgcaa acctgtacgg atggtgtacg tcccacaatg ctgccaccgc
     3541 atcacttgcc acactatcta atagtctgca caagcgggat gtatcggaat agacaccatt
     3601 agtttcaaca agcaatgtca cacctttttc ttccgcaata ggggtcagca tcttaagtgc
     3661 ttccaaaacc actttatcat ccacatctcc gcatggttgg ggctcaagat ctgcaagaat
     3721 acgcacataa ggcgtcccca gccttgacgc aagagtaata tattctacta tctcttcatg
     3781 atttttttga gcattctcag caaacttcaa gcaacaacct gaagaaaaac atggaatctc
     3841 aaggcgaagt tccgcaagtt ttttcgctgt atgggataac tgggattctg taaaaggctt
     3901 tgcttttaca gcaaagattt catttcccaa tccccgtatt tcaataccgt caaaaccaaa
     3961 atccttagcc atggaatata tttcgggcca gctaaagtca gggcatccca gagttgaaaa
     4021 tccaattttc ataaaaatcc tccttaatta cttaaaaatt ttattattaa aattaaccgg
     4081 ctattctaca aagccttaat aaactaaaaa tctaaagctc tcgggtatcg aacttctttt
     4141 ttcggcaatt gactttgtga aattcagcta aataaaaaaa gcctccgtct cataaattta
     4201 ttcaagagac gaaagcattt tactcccgtg gtaccactct tattggtatt aatacccact
     4261 cagtgacatc aacaacaatc aatgtcctct catgataacg gcgagatacc cgttcccacc
     4321 tacttgccaa acagacaaaa tctgaaaagc tttcagttga atgctcaaag gcgagtttca
     4381 caatctgctc cgtgtactgt tttccagcaa ccaacagctc tctgagacac taacaagcag
     4441 cgttttctcc tcatcaacgc attttaacct ttttcattta aatcattttt ttgttatggt
     4501 atcaaaaata atattacttg tcaagatgca ttatgtctac tttttcgaag agttttccac
     4561 ttttcttttt tcaatattct cccatataac caatattaca gccccaatca aatagcacaa
     4621 aatatctttt acatcaaagg aagttccgat aattgcagaa agtattttat tactccttaa
     4681 atgcaatatg tctacaattc ggtattattg agccatctcc acaataaacg caaaagcaaa
     4741 caggtatatt ggcaaaaact ttatgggttt tccaacaatt cctctaatga aagtatacat
     4801 taatataacc accagtatat cccctacgta tggtcttatg atggcatccc tgacaaacac
     4861 accgataata atttcaatta tcaaaagaat tataaaagca aatatgtatt tcgggtttat
     4921 tttcatttat ttaccacctc acttatttac ccaatatcgg ttgcacaaaa taatataccc
     4981 accctacatc aaaggtgcaa aaattctaag aacagtgcct atcagtcttc tgtaccattt
     5041 catttctttt atatctttca tggttatttc cttgcatgct ttaagggtat cgagaaaatc
     5101 ttccttaaca gccagaacac ttccgctttt atacatccac acaccgcatt caaaatgcag
     5161 gtaaaaactc ctatagtcca tgtttatcga tcctatgact gcatactcgt catcggaaac
     5221 caaggttttc gaatgaataa accccggggt gtactcatat atttttacac cgttttctat
     5281 taaggccctg taattcgatc ttgtaactgc atgtacatac catttatccg ccaggtacgg
     5341 agttatgata cggacatcta ctccggattt agccgcagca gtcaatgctt tagtcattcc
     5401 atagtcaata accagataag gagttgtaat ataaacatat ttatttgatt tgttcaataa
     5461 attcaaatat actatttcac cgacagtttc atcgtcaaaa gggctatctg caaagggctg
     5521 aaaataccct tgtgtgcttt ctgttttaaa aggattattg ttcttaaacc tggtaaaatc
     5581 ctcacctact ccctttagat aatcccacat ggaaagaaac attaccgtca tattccatac
     5641 agcttccccc ttaatcatga tggcattatc cttccaatat ccgtgccttt ctatcttatt
     5701 tatatattca tctgcaaggt taatgccgcc cgtgaaacca gtatgtccgt caattatcgt
     5761 tattttgcgg tgatcccggt tgtttatttt agcagacaga acaggtctta ccggattaaa
     5821 aacacaacat ttaattccaa ggtgctccaa tttgtgataa tagtcccgcg gtaaaagcaa
     5881 aaggcaaccc aagtcgtcat atattatacg tacatctaca cctttttttg ctttttcagt
     5941 caatatatcc agtatgctgt tccacataac cccttcctta atgataaagt attccatgaa
     6001 aataaagtgc tccgcttttt ttaattcttc cttcagcctt tcaaacttta gttctccttt
     6061 tgggaagtat tcaccggttg tgttctgata gggcggaaaa aaagcaatat tttgaatata
     6121 tcttgattga gcaaccgcac ccgtgtcatg cggcatcatt ttctccaata cgtggggctg
     6181 gggaaccaaa gcattttgta ttttcagtcc gattgacatt aatcttttct tttcacgttt
     6241 gctcaatttt ttaccgccaa aaaagatgta aaacaagcct ccaaaaatag ggaatagcaa
     6301 aatgggaatt atccatgcaa ttttgtatga aggattgctt ttatcattta gtatccagaa
     6361 tactgtaaat aagctgacaa atatacttcc ccaataaaac aagacaaagt agttgttaaa
     6421 ccttataatg acagctatta acacagccag ttgtattaca atagctaaag atatcagaac
     6481 aactctatga tagaagaacc ttataatcgt tttaatttta tgccacatat gagtcctcat
     6541 cccctgggaa ttcaatccat aattttgaga tactatatat tactgttctc taaatttcaa
     6601 caaaaacctt tagactatca atcaaattat gcaatataaa tataagatta caactttttt
     6661 cattcattac aaagttggta tcttgtaatg actgtaaaaa gaatggaatt taccgtcata
     6721 gtatatcctc tatgctctga attatttaaa aatacatctt ttgaagagtt tatactttta
     6781 tagtttcaat agagtagaga aataattgct aagaattatt tccctctcgt tgaaccgtac
//
LOCUS       DAOOJC010000077         6829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_9249_length_6829_cov_3.522439, whole genome shotgun sequence.
ACCESSION   DAOOJC010000077 DAOOJC010000000
VERSION     DAOOJC010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9249_length_6829_cov_3.522439"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..581)
                     /locus_tag="PLA01_03350"
     CDS             complement(177..581)
                     /locus_tag="PLA01_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HPD00367.1"
                     /translation="MVLKDLIEKRRKERKKAARRKTVKNTAIGAGVGAAVGLAAGVLL
                     APKSGKETRQDIAENTKRAAGFVSENTKKAVEKVSEASRKAVDTVKESINDVKGKITE
                     FRASKEESVEEVAVTEEVKEETEDKEKGKRKK"
     gene            complement(714..1586)
                     /locus_tag="PLA01_03355"
     CDS             complement(714..1586)
                     /locus_tag="PLA01_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPD00368.1"
                     /translation="MNCEKVLELMEEYIRGELEESLNKKVEEHILSCDTCKIEYSETK
                     EIVEALCNLKKSIRIKEDVFDMNKRSIVNKAEKRGRKPLRGYISGIAAAVFFVMFLFA
                     GSIIAFPTFASSYMPELPMVKQFKEAQEENNAVKDEIEKVKQQNEEIMAENEAIRKEN
                     DELKSKLKEIGGSLITEYQTSEGIGEEDNNRIQEMAIAFIKAQYRGDTEAIKQMCTDE
                     LRKKVERNPAQYLLDDKGNVVFTQITNVAKEGDLYLVYVRLSDDSDISSYQINFEIVK
                     ENDEFLVNFAGYDA"
     gene            complement(1583..2110)
                     /locus_tag="PLA01_03360"
     CDS             complement(1583..2110)
                     /locus_tag="PLA01_03360"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPD00369.1"
                     /translation="MEIDMLIEAAKRGDKKAFESIVDRYHNELYYTALGILRSGWDAL
                     DVCQETFLKVFSSLDTLKDTSKFRFWMNRILINKCNDHFRRNKKVTVVEHIENEGFIE
                     QGKEENIDLLKALSSLKEDARIVLTLRYFQDMPVKEIASLLGIPEGTVKSRLSYGLKE
                     LRKLMKVSRKGDESI"
     gene            complement(2257..3207)
                     /locus_tag="PLA01_03365"
     CDS             complement(2257..3207)
                     /locus_tag="PLA01_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00370.1"
                     /translation="MNKKGRGIGLGMFLIFIGVIAVMIHFDKLSVWGMFGFVRNYVSM
                     LLAMLLILTGINIVLRKYAFVKVITWGGFFTVLALAAYNFNINYNERNKEEIKNDYGK
                     PFAVEKNPETEEAKLNLNLAGFKLKIGSTEANLVEGVLTDPNLKYEVDYKDGKKVAKV
                     DFKSKSNISIYGLDDILELRGLAMEKPSEILINEDVVWDIELDAGAIESNIDLSQIKV
                     KNLNMDGGAGSFKVVLGEKYSSTEVDIDTGASELEFYVPKTSGVKLNIDGLINSKDFK
                     GITLEKKGDHYITPNYDKTENKIEINIDMGVGSLKINGVD"
     gene            complement(3204..3668)
                     /locus_tag="PLA01_03370"
     CDS             complement(3204..3668)
                     /locus_tag="PLA01_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPD00371.1"
                     /translation="MKRRLVRSKNDKVLAGVCGGIAEYFDIDPTIIRLGFVILTLVGP
                     GILVYLLAIFIIPEDDGSSYKPSNFFNDDDSFAQNEYDPEKDFSNVMGDRDDDYIRNP
                     EKNKTFIGICLIILGAVFLAKQFIKIDLKLIIPLILIGIGALILFKGGRRLP"
     gene            3949..4524
                     /locus_tag="PLA01_03375"
     CDS             3949..4524
                     /locus_tag="PLA01_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPD00372.1"
                     /translation="MKKVCLKELFEIFITFFKIGAFTLGGGYAMLPLIENEVVEKKKW
                     ATQEEIIDIFAIVQSVPGVVAVNTSIFIGYKRAKLKGGITAVLGVVLPSFLIILAIYQ
                     LLFSLSNNIYAIKAFGGVRAGVTALILVTAIKLIKTTIKDVISAVIAIISFVLILFFD
                     NDIFIILAGVTLGIVRHLVSNIKVLRKEQKS"
     gene            4521..5129
                     /locus_tag="PLA01_03380"
     CDS             4521..5129
                     /locus_tag="PLA01_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPD00373.1"
                     /translation="MIYWLLFWTFFKIGAVSFGGGYAMIPLIQEQLEALGWMNAEEFA
                     NILAVSEMTPGPLATNTATYVGAKTAGVLGSMAATIGVTFPSLIIITLVARFFIQFKE
                     NRMVNSILQTIRPVTIGLISSAVVFISQTSLLKSEIQLPGFFDVLKGNISGIINSIQP
                     NFAGIAIFIIILVAIAKYKLHPIFAIMFSALLGIASYLVFGV"
     gene            5502..>6829
                     /locus_tag="PLA01_03385"
     CDS             5502..>6829
                     /locus_tag="PLA01_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 48 protein"
                     /protein_id="HPD00374.1"
                     /translation="MAAVAIITTAALVMTNGNISNPSGSISVPNKTKPSTKASTVYSD
                     RFIKLFEDIYTKGYLSKEGIPYHSIETLIVDKPDYGHLTTSEAFSYMVWLGATYGKLT
                     GDWSYFTDAWDKTEQYIIPDPQKDQPGVDIYSPKKPAQYAPEANSLRGYPVAVSESAP
                     TGIDPITDQIASVYGSKAVYRMHWLLDVDNWYGYGNHGDGTSRCSYINTYQRGPEESV
                     WETVPHPSWEDFKWGQVNSSGFLSLLNSSIQPSNQWRYTSAPDADARQIQATYWAYLW
                     SKEQGINKELNPYFEKAAKMGDYLRYSLFDKYFRPIGVQNGSGAGKGYDSCHYLLSWN
                     TSWGGDISGTWSWRTGNSHCHQGYQNPVAAFALSNEAALNPKSKNAKKDWEKSLERQL
                     ELFQYLQSSEGAIAGGVTNSWNGKYEKYPEGTSTFYEMAYDWQPAYQDPP"
BASE COUNT         2064 a         1490 c         1050 g         2225 t
ORIGIN      
        1 aacgtgtaca aaatgaaatt cgccagttca tacgaaaccg ttatttgcat tttgcttccc
       61 cctttgttta tttccgtcaa aaagctcagg ggcattaagc ccccttccgc cttttgcccg
      121 gttttacttc gcgaaacaat ccgaacaatt acagttctat tgtaaaataa aaaaaattac
      181 ttttttcttt tacctttttc tttatcctca gtttcctctt tcacttcttc cgttactgcc
      241 acttcctcaa cactctcttc tttgcttgcc ctgaattcag taatttttcc ttttacatca
      301 tttattgatt ctttaacagt atccacagcc tttcttgaag cctcagaaac cttctcaacg
      361 gctttctttg tgttctcaga gacaaatccg gcagccctct ttgtgttttc agcaatgtct
      421 tgtctcgtct cttttcctga cttgggtgct aacaaaacgc ctgcagccaa tccaactgcg
      481 gcccctactc cagctcctat tgcagtattc ttaaccgtct ttcttctagc agcttttttt
      541 ctttcttttc ttcttttctc aattaagtcc tttagtacca cgtcaaagca cctcctgttt
      601 attttacata aaaaaatgca cctatatata ttatacgata ctttggagaa aattcctagt
      661 gacaatatta catttttaca acattgccac cgtttatgaa agcctttatt atgttatgca
      721 tcatagcctg caaagtttac aaggaattca tcattttcct taacaatttc aaaattaatt
      781 tgatagcttg agatgtcact gtcatcactt aatcttacat agacaaggta tagatcgcct
      841 tcctttgcaa catttgttat ttgtgtaaat accacatttc ccttatcatc taacaaatat
      901 tgagctgggt tacgctctac ttttttccgg agctcgtcag tacacatttg ctttattgcc
      961 tcagtatctc ctctatactg tgccttgata aatgcaattg ccatctcctg tatcctgtta
     1021 ttatcctctt cgcctattcc ttcactggtc tgatactcag taataaggct tcctcctatt
     1081 tccttcagtt tagatttaag ctcatcgttt tctttcctga ttgcttcatt ctcagccata
     1141 atctcctcat tctgctgttt taccttttca atctcatctt ttactgcatt attctcttcc
     1201 tgagcttctt taaactgttt taccatcggc aactcaggca tatagcttga agcaaaggtc
     1261 ggaaaagcta taattgaacc agcaaaaagg aacattacaa aaaataccgc tgcagctatc
     1321 cctgaaatgt agccccttaa tggctttctg cctcttttct ccgctttatt tactatactc
     1381 ctcttattca tatcaaaaac atcctccttt attcttatag attttttcaa gttacataaa
     1441 gcctcaacaa tttcttttgt ttcgctgtat tctatcttac aagtgtcaca gcttaaaata
     1501 tgttcctcaa cttttttatt caagctctcc tcaagctctc cccttatata ttcttccatc
     1561 agctctaaaa ccttttcaca gttcatatac tctcatcacc cttcctactg accttcatca
     1621 gttttcttaa ttccttaagt ccataactta aacgggattt caccgttccc tccggaatac
     1681 ccaacaaaga agcaatctct ttaacaggca tatcctgaaa ataccttaac gtcaatacaa
     1741 ttctggcatc ctcttttaac gaagacaatg ccttcagaag atcaatattt tcttcctttc
     1801 cctgctcaat aaagccttca ttctcaatat gttctacaac cgtaaccttt ttatttcgtc
     1861 tgaaatgatc attgcattta ttgatcagta ttctgttcat ccagaaccta aacttagatg
     1921 tatcctttaa tgtatccaga gaagaaaaca cctttaaaaa cgtctcctga catacatcaa
     1981 gagcatccca tcccgagcgt agaattccaa gtgctgtata gtataactca ttatggtatc
     2041 tgtcaacaat ggattcaaaa gcttttttgt cgccacgttt tgccgcttct atgagcatat
     2101 caatttccac caccacacct ccatcttttt ttatacacaa taatgacgta ctgccccggc
     2161 aattggttca aataaaataa accaataaaa aaagcttaag aacagtaaac aaaatactgc
     2221 tcattaagca ctcaacttct ggcctataca catcgattag tcaacaccgt taatcttcag
     2281 agaacccaca cccatatcta tattaatttc aattttgttt tccgtcttat cgtaattggg
     2341 agtaatataa tgatcacctt tcttttcaag ggttatgcct ttaaaatctt ttgaattaat
     2401 cagtccgtca atattcagtt ttacacctga agttttggga acataaaatt caagctcgga
     2461 tgcacctgta tcaatatcaa cctctgtaga agaatatttc tcccccagca ccactttaaa
     2521 gcttccggca cccccatcca tattgaggtt tttaactttt atctgagata gatctatatt
     2581 actttcaata gctccggcat ccaattcgat gtcccacaca acatcctcat ttatcaatat
     2641 ctccgatggt ttttccattg ccagtcccct taattctaaa atatcatcca gcccgtatat
     2701 cgaaatattg gatttggatt taaaatcaac tttggcaacc tttttaccat ctttatagtc
     2761 cacctcatat tttaaatttg ggtcggttag tactccttca acaagattcg cttctgttga
     2821 accgatttta agcttgaacc ctgcaaggtt cagatttaac ttcgcttcct ccgtctcagg
     2881 atttttttcc acagcaaacg gcttgccata gtcatttttt atttcttcct tatttctttc
     2941 gttgtaatta atattaaagt tataagccgc caatgccaat actgtaaaaa aaccccccca
     3001 ggtaataacc ttgacaaaag catatttcct caaaacaatg tttatcccgg ttaatataag
     3061 caacatggcc aaaagcatcg atacataatt acgtacaaat ccaaacattc cccacacgct
     3121 taacttatca aagtgaatca tcacggctat cacaccaatg aaaattaaga acattccaag
     3181 gccaatacct ctcccctttt tattcatggc agacgcctcc cgcctttaaa tagaatcaat
     3241 gcaccgatac ctataagaat caacggaata ataagtttaa ggtcaatttt aataaactgc
     3301 tttgcaagaa aaacagctcc aagtattata agacatattc caataaaggt tttattcttt
     3361 tccggattgc gaatgtagtc atcatccctg tctcccataa cattactaaa atccttttcg
     3421 ggatcatact cattttgggc gaagctgtca tcgtcattaa agaaattgct gggcttatag
     3481 cttgagccat catcttcagg aataataaaa atagccagaa gataaacaag tattcccggt
     3541 cctactaaag ttaaaattac aaaccctagc cttataatag taggatcaat atcaaaatac
     3601 tctgcaattc caccacaaac tcctgctaaa actttgtcgt tttttgaacg gaccaacctg
     3661 cgtttcatgt ccttttcctc cttattggct aacaatccat cgttattgta tacatgcctg
     3721 tcagaattcc aattatacct ggttattctc tcaatacaac atgtatactt cttattttta
     3781 taatacgaat aattcaacaa aaagtcttaa tacttataaa aataatttaa caattatccg
     3841 ctgtaaataa atgcaataaa aaatctctta aaacccttta acaaaacaaa ttttattgtt
     3901 atcatataac aagtgcaaaa aatactatgg gttttaaggc aggtttctat gaaaaaagtg
     3961 tgtcttaagg aattattcga aatttttatt acatttttta agattggtgc tttcacgttg
     4021 ggtggaggct atgcaatgct ccctcttatt gagaatgaag tcgtcgaaaa aaagaaatgg
     4081 gcaactcagg aggaaatcat agacatattt gctattgtcc aatcggttcc cggagtagtc
     4141 gcagtaaata cctcaatatt tattggatat aaaagggcaa aactcaaggg tggtatcaca
     4201 gcagtattag gagttgttct tccctctttt cttattattc ttgccattta tcagcttttg
     4261 ttcagcttaa gcaataatat atatgcaata aaagcttttg gcggagtgag ggcaggtgtt
     4321 accgcactta ttctcgtaac agcaataaag ctcataaaaa ccacaataaa ggatgtaatt
     4381 tcagcagtta tagccattat ctcattcgtc ttgatattgt tttttgataa cgatattttt
     4441 ataatattgg ccggagtgac ccttgggata gtacgacatc ttgtatcaaa tattaaagta
     4501 cttagaaagg aacaaaaatc atgatttatt ggctattatt ctggacattt ttcaaaatcg
     4561 gagccgtcag ctttggaggc ggttacgcaa tgattcccct tatacaggag caactcgagg
     4621 ctttgggttg gatgaatgct gaggaatttg caaatattct ggctgtatca gaaatgacac
     4681 cgggtccctt agccaccaac actgcaacct atgttggtgc caaaacagca ggagttttag
     4741 gttcaatggc agcaaccata ggggttactt tcccatcgtt gattattata acgctcgttg
     4801 cccgcttctt tattcaattt aaagaaaata gaatggtaaa ttccatttta caaaccatac
     4861 gaccagttac cataggctta atttcctctg cagtagtttt tatttctcaa acctcattac
     4921 ttaaatcaga gattcagttg ccgggtttct ttgacgttct aaaaggcaat atatcgggca
     4981 taataaactc catacaaccg aactttgcgg gtattgcaat attcataata atccttgtag
     5041 ctattgcaaa atacaagctg catccaatat ttgcaattat gttttccgca ctattgggga
     5101 ttgcttcata ccttgtattt ggggtttaat ccaggctgtg tacttacaaa actgaaataa
     5161 attaaaacat ccttcttcac attaaccggt acacccgaaa tcagtgcata atcctgtccc
     5221 gcgaatctta tgtttgtcaa atgcctttct taacatttat ttaatgaacc caaactatag
     5281 ttaataattg aagaaatatt aaataattag tataatttat attagatgtg tcaagcattt
     5341 aatataattt atacttgaga acatttattt tcagggggag gtaaattgta taatgtacac
     5401 cgggcgttaa ttcaaaaggt gtgcatatac aaaagaagct tatgaaatta atatataatg
     5461 taaagcgaag aaggaaaatc ttcatatttg gtttatgcag cttggcagct gttgcaataa
     5521 ttactacagc agcacttgtc atgacaaacg gaaacattag taatccatcc ggttcaatat
     5581 ccgttcccaa taaaacaaaa ccttctacaa aagcctcaac tgtttattcc gataggttca
     5641 taaagctctt tgaagatatt tatacaaaag gctatcttag taaagaaggt attccttacc
     5701 actcaattga aactcttatc gtagataaac ccgattacgg tcatcttaca acaagtgaag
     5761 cattcagtta tatggtatgg cttggggcca catacggaaa acttaccggt gactggtcat
     5821 actttacgga tgcatgggat aaaacagagc aatatataat tcccgatcct caaaaggatc
     5881 aacccggtgt tgatatttat tcaccgaaaa aaccggcaca atatgcacca gaagccaatt
     5941 ctttaagagg ctaccctgtt gccgtcagtg agagtgcacc caccggtata gatccaatta
     6001 ccgaccagat agcttctgtc tatggctcaa aagctgtata ccggatgcat tggcttcttg
     6061 atgtggataa ctggtatgga tatggaaatc atggcgacgg tacaagccgc tgctcctata
     6121 taaatacata tcaaagggga cctgaagagt cggtttggga aacagttcca catccctctt
     6181 gggaagactt taaatggggc caggttaata gcagtggatt tctttcttta ctcaatagtt
     6241 ccattcaacc ttcaaatcaa tggcgttaca cctcagcacc ggatgctgat gcaagacaga
     6301 ttcaggcaac ttattgggca tacctctggt ctaaagagca aggaataaat aaagaactta
     6361 acccctactt tgaaaaagct gcaaaaatgg gagattacct tagatattct ttgtttgaca
     6421 agtatttcag acctatagga gttcagaatg ggtcaggtgc cggcaaaggc tatgatagtt
     6481 gccattatct tctttcctgg aatacatcgt ggggaggaga tattagcgga acctggagtt
     6541 ggcgaaccgg aaactcccat tgtcatcagg gatatcaaaa tcccgttgcc gcttttgcat
     6601 tgtcaaatga ggctgcactt aatcctaaat caaaaaatgc caaaaaggac tgggaaaaaa
     6661 gtcttgagcg gcagttggaa ttattccaat accttcagag ttctgaaggt gccatagcag
     6721 gtggcgttac caatagctgg aatggaaaat atgaaaaata tcctgaggga acaagtacat
     6781 tctatgaaat ggcatacgat tggcaaccgg cttatcaaga ccctcccag
//
LOCUS       DAOOJC010000078         6822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_9266_length_6822_cov_3.733708, whole genome shotgun sequence.
ACCESSION   DAOOJC010000078 DAOOJC010000000
VERSION     DAOOJC010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9266_length_6822_cov_3.733708"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PLA01_03390"
     CDS             <1..338
                     /locus_tag="PLA01_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPD00375.1"
                     /translation="GAAAVYTPEGQKQIKATIDYYMTNAKIIKKGLEDVGLTVFGGVN
                     APYIWLKTPAGLSSWEFFDKLLKEINVVGTPGSGFGPSGEGYFRLTAFGSRENTEEAV
                     ERFKKGLRL"
     gene            561..1484
                     /locus_tag="PLA01_03395"
     CDS             561..1484
                     /locus_tag="PLA01_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPD00376.1"
                     /translation="MKLRKGIFYICAVLSLILFIQGVYVLLFTDEEGSRADFQSKYSP
                     IQIIRKNTYDDNYGKKNACINLLLLGLDEEETRSDVIALINYSQEKAKMNVLSIARDT
                     RIKADGKTMKINALIAKGGEKAVIDIVEDITGLKVDYYITLNFRGFRKIIDTLGGVKI
                     EVPFDMDYDDPYQNLHIHLKKGEQVLNGKEAEGFVRYRKSNKGEGYENGDIGRIEMQH
                     QFISEFIRQKFKMKYLMKADDIFYILKDNMKTNVEIGDLRYFIKDIGNLKVPEIKSFV
                     LPGYSKYIGNQWYYIYDKKKTDMLIKDYFYY"
     gene            1749..2735
                     /locus_tag="PLA01_03400"
     CDS             1749..2735
                     /locus_tag="PLA01_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HPD00377.1"
                     /translation="MKRIGFTTSIPVEVIFAAGDRPVDLNNVFITNDNPGRLVEIAEN
                     AGFPRNTCAWIKGIYSAIMDGGDIDTLIGVVEGDCSNARALVEVLEQKGVDCISFSYP
                     NSRDYDQLKKEIVRLCGYFGVELEDCTKVKKELDTIRQRLIYLDELTWKHNKATGFEN
                     HIWQVSSSDFNGDYVSFGRELDEKIASVDKRAPKTGGKRIGYIGVPPINGDIYDFVES
                     LNARVVYNEVQRQFTMADSVGIEDVVEVYRNFTYPYSLRGRLLDIKDQISKRKIDGII
                     HYAQAFCFRGIEDIVIRKELNIPVLTIEGDRPGNLDQRTKLRIEAFIDMLDQ"
     gene            2811..3590
                     /locus_tag="PLA01_03405"
     CDS             2811..3590
                     /locus_tag="PLA01_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPD00378.1"
                     /translation="MRLLGIDLGSRAVKMALFEEDRLIESRIYDTAAFYRDYCKNTNQ
                     RISIDFDRLNFGGFDQIISTGYGRNNVNVENAMVIVELKAHTFGAVWQTGLQNFTLLD
                     IGGQDSKVISVKNSRMVDMVLNDKCAASCGRYLENMAGVLGIGLDELVRHHENPVELS
                     STCAVFGESELIGKISEGYGIGELAAGVNYSLFKRIKPIVERFPDDNLVVSGGVARNE
                     ALISFIGEELGFKQIVIPERPQLNGAIGCCAYYIAASRNNS"
     gene            3711..4379
                     /gene="queC"
                     /locus_tag="PLA01_03410"
     CDS             3711..4379
                     /gene="queC"
                     /locus_tag="PLA01_03410"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HPD00379.1"
                     /translation="MKKAVVLLSGGLDSTTCLSVALKDGYEVYPLSFDYGQRHYRELE
                     SAKKVAEYYNIKSHKIVKMGNVGGSALTDAGIDVPDYKGTADIPVTYVPARNIVFLSY
                     AAGYAEVVEAEAIYIGVNAIDYSGYPDCRPEFIEAFEKVIEVGTKRGVEGKAVKIVTP
                     LINLSKAQIIKLAYENNAPLHLTTSCYRGGEKACGICDSCVLRLKGFEEAGIDDTIEY
                     LKRI"
     gene            4399..4782
                     /gene="queD"
                     /locus_tag="PLA01_03415"
     CDS             4399..4782
                     /gene="queD"
                     /locus_tag="PLA01_03415"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HPD00380.1"
                     /translation="MKIGQVAISKVFTFDSAHRLVGYNGKCKEIHGHTYKLEVTVKGK
                     PDSDGLVMDFNELNEIIKNEVLEKIDHKYLNDVFDFNPTCEMIGLWIWEQVKKSIDKT
                     NCSLEKLVLWETPTSFITIDRKDME"
     gene            4800..5432
                     /locus_tag="PLA01_03420"
     CDS             4800..5432
                     /locus_tag="PLA01_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD00381.1"
                     /translation="MKVNEIFLSIQGESLSTGFPTIFVRFSGCNLRCSFCDTTYAYDS
                     GRDMTPNNIYEEIKKLHYKRVCLTGGEPLMQEDLSELLVLLEDYIITIETNGSLELKK
                     VKLVSPKHSYVMDMKVPSSGCSDRMVFSNFDELREQDEIKFVIGDRNDYEWSKDVISR
                     FHNKGVITFSPVYGKIDYSEVVRWILDDRLDVRFQVQLHKIIWGPYKTGV"
     gene            5438..6007
                     /locus_tag="PLA01_03425"
     CDS             5438..6007
                     /locus_tag="PLA01_03425"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPD00382.1"
                     /translation="MYNWEELIEACENCNKCELGKTRKNIVIGRGNRNAPMMLVGEGP
                     GEQEDIQGLPFVGAAGKLLDLLLKGMRIPEDMYYIANIVKCRPPYNRVPTDIEAETCL
                     PYLRNQVFLIKPKIIVCLGATAMKYIVDRNARITQIRGQWIERKGYWIMPTFHPAALL
                     RDESKKILMWQDFKRVKQRLDDICLNQSS"
     gene            5986..6561
                     /locus_tag="PLA01_03430"
     CDS             5986..6561
                     /locus_tag="PLA01_03430"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPD00383.1"
                     /translation="MSKSEQLKNLYELYEKVFYGKEIVFGDGNPDTRLILIGEAPGKD
                     EVQQGKPFVGAAGKNLSEFLDRLGIIREDIYITNAIKYRLSKVNPETNRITNRPATKK
                     EIEENREYLLKEIDVINSEYIVTLGNVPLRAICGNNVTIGELHGRKNKIEVLGNEYVL
                     FPLYHPASVIYNVRLKEVYYEDLEKLKKIIE"
BASE COUNT         2211 a         1014 c         1719 g         1878 t
ORIGIN      
        1 agggggcggc ggcagtttat acgcccgaag gacaaaagca gattaaggca acaattgatt
       61 actacatgac caatgcaaaa ataataaaga aaggtctgga agatgtaggc cttactgtat
      121 tcggcggggt gaatgctccg tatatctggc ttaagacacc ggcagggctc agctcctggg
      181 aattcttcga caagctgctc aaggaaataa atgtggtcgg aacaccaggc tctggttttg
      241 gtcctagtgg agaaggatac ttcaggctaa ctgctttcgg aagcagagaa aatacagaag
      301 aggcagtgga aagattcaaa aagggtttaa gactgtaata gcggtgatat gtggaaagag
      361 cactttcctt catacttatt tatttatgta ccttacaggc attttctttt ggaagatgcc
      421 tgtttttcaa ttgacggaat ctcacgaagc gaaaggagca agcatataat caagcatata
      481 attgtattaa aaataccaat acacatatac taagtatgag tgattattat tgctaaattt
      541 tgctttcaga caggtgagat atgaagctta gaaaaggtat tttttatatt tgtgcggtat
      601 tatcgttgat tttgtttatc caaggtgttt atgttctttt atttactgat gaagaaggga
      661 gcagagcgga ttttcagagc aagtatagcc ctattcagat aatccgcaaa aacacttacg
      721 atgataacta tggaaagaag aatgcatgca ttaaccttct cttgttgggc cttgatgaag
      781 aagaaacccg atccgatgtt attgcattaa taaactatag ccaagaaaaa gcaaaaatga
      841 atgtcttgtc cattgcaagg gatacaagga taaaggctga cggcaagacg atgaagataa
      901 acgcactgat tgccaagggc ggagaaaaag cagtaattga tattgtggaa gacataaccg
      961 ggttgaaggt ggactactat ataaccctga actttagagg ctttagaaag attattgata
     1021 cactgggggg tgtaaaaata gaagtaccct ttgatatgga ttacgatgat ccctatcaga
     1081 acctgcatat ccatttaaag aagggagaac aggttttgaa cggaaaagaa gcagagggat
     1141 ttgtacgtta ccgtaaaagc aataaaggtg aaggctacga aaatggggat ataggcagaa
     1201 tagaaatgca gcatcagttt atttcggagt tcataaggca aaagtttaaa atgaagtatc
     1261 ttatgaaggc agatgacatt ttttatatac tgaaagataa catgaaaacc aatgtggaaa
     1321 taggagattt acgctacttt ataaaggata tcggcaattt aaaagttccg gaaatcaaat
     1381 cttttgtcct gccgggttat tcaaagtata tagggaatca gtggtattat atttatgata
     1441 aaaaaaagac cgatatgctg attaaggatt atttttatta ttagacaagg ttttaagcac
     1501 ttcttaaaat gtcttataaa gctatacttg cttttataaa attcaattgt gtattataat
     1561 atatatgaat tgcatacaat ccttcatatg ggcttgttgc aataaatgta tcggtgtact
     1621 tgttatttcg gtatgtatac cgataagctc ccgcttttac cggcgggcta gttcgatata
     1681 aatttgatat tcggtaagat attggttttt gctgaattta catgttttat aagcataggg
     1741 ggactttttt gaaaagaatt ggttttacaa caagcatacc cgttgaggta atatttgcgg
     1801 caggtgacag accggtcgat ttaaataatg tctttattac aaacgataat cccggaaggc
     1861 tggtagagat tgctgagaat gcagggtttc ccagaaacac gtgtgcatgg ataaaaggta
     1921 tttattctgc tattatggat gggggagaca tagacacgct gattggtgtc gtggagggag
     1981 attgttccaa tgcaagggca cttgtagaag ttcttgagca aaaaggggtt gactgtattt
     2041 ctttttcata cccgaattcc cgcgactatg atcagctgaa aaaggaaatt gtcaggctgt
     2101 gcggttattt tggggttgaa ttggaggatt gtacaaaagt taagaaagaa ctggatacta
     2161 tacggcaaag gttgatttac ctggacgaat tgacgtggaa gcataacaag gcaacggggt
     2221 tcgaaaacca tatatggcag gtttcaagca gcgattttaa cggggattat gttagctttg
     2281 gaagagaact ggacgaaaaa atagcatccg ttgataaaag ggctcccaaa accgggggaa
     2341 agagaatagg ttatataggt gttccgccca ttaacggaga tatttatgat tttgttgagt
     2401 cgctaaatgc cagggtggtg tataatgagg tccaaagaca gtttacaatg gcggattctg
     2461 tcggtataga ggatgtcgtt gaggtttaca ggaatttcac atacccctat agcctcaggg
     2521 gaaggctttt ggatatcaag gatcaaatat ccaaaaggaa aatagacggt ataattcact
     2581 atgcccaggc tttttgcttc aggggtattg aggatatagt aataagaaag gaattgaaca
     2641 ttcccgttct taccatagag ggagacaggc cgggaaacct tgaccaaagg acgaagctta
     2701 gaattgaggc atttatcgat atgctggatc agtgaatttt tgactaagag agttttttca
     2761 atggataagt cagatagagg atgaatattt aaatgcttag gagtaacatt atgagacttt
     2821 tggggataga tttgggcagc agagccgtta aaatggcact ctttgaggag gacaggctta
     2881 ttgaaagccg gatttatgat acggccgcct tttatcgtga ttattgcaaa aatacaaatc
     2941 aaaggataag cattgacttt gacaggctta actttggggg gtttgaccaa ataataagca
     3001 ccggctacgg gcgaaacaat gttaatgtag aaaatgccat ggttattgtg gagttgaagg
     3061 cacatacctt cggagcggta tggcaaactg gccttcaaaa ttttaccctt ttagatattg
     3121 ggggccagga cagcaaagtc atatcggtga aaaactccag gatggtggac atggttttaa
     3181 atgacaagtg tgccgcatcc tgcggcaggt atttagagaa catggcaggt gtcctgggga
     3241 tcggtttgga cgagcttgta aggcaccatg aaaaccctgt cgagcttagc tctacctgtg
     3301 cggttttcgg tgagtcggag ctgataggaa aaatatccga aggatacggc ataggggagt
     3361 tggctgcggg ggtgaattac tcgcttttta agaggatcaa gccgatagtt gaaaggttcc
     3421 ccgatgacaa cctagtggta tccggcggcg ttgcaagaaa cgaagccctg ataagtttta
     3481 tcggtgaaga actggggttt aaacagattg taatacccga aaggccgcag cttaacggtg
     3541 ctatagggtg ttgtgcgtat tatattgctg caagcaggaa taacagctag aggcttcaaa
     3601 ccggcaactg ataaatagca gcttattatg ctgtatataa atttttgtta ttaaacgcat
     3661 attcaggatg acaattgcct ttttgcgttc aatatttata ggaggatatt atgaaaaaag
     3721 cagttgtatt gctttctggg ggacttgaca gtactacatg tctttcggtt gcattaaaag
     3781 atggttatga ggtgtatccc ctgtcctttg actatgggca gaggcattat agggaactgg
     3841 aaagtgcgaa aaaagtagca gaatattaca atattaagag ccacaaaatt gtgaagatgg
     3901 gtaatgtggg cggaagtgct ttaaccgatg ctggtataga tgttccggat tataagggaa
     3961 cagccgatat acctgttaca tatgtgccgg ccagaaatat tgttttctta agctatgcgg
     4021 caggatatgc cgaggtggtg gaagcagaag cgatatatat tggtgttaat gctattgact
     4081 acagcggata tcccgattgc agacctgagt ttattgaggc ttttgagaag gtgattgagg
     4141 tgggaacaaa gcggggagtt gaaggaaaag ccgtaaaaat agttacaccg ttgatcaatc
     4201 tttcaaaagc acagattatt aagctggcat atgaaaataa tgctcccctg catttgacaa
     4261 caagctgcta tcgcggggga gaaaaggcct gcggcatttg tgacagctgt gttttaaggc
     4321 taaagggatt tgaagaagct ggaatagatg atactataga atatttgaag agaatataat
     4381 tttgagagga atagagatat gaaaattgga caggtcgcaa ttagcaaggt ttttacgttt
     4441 gacagtgccc atcggttggt aggctataat ggaaagtgca aggagataca cgggcatacc
     4501 tacaaacttg aggtaactgt aaaaggcaag ccggacagcg atgggcttgt tatggacttt
     4561 aatgaattaa atgaaattat aaagaacgag gtactggaaa aaattgacca caagtacctt
     4621 aatgacgtgt ttgattttaa cccgacatgt gaaatgatag ggctttggat atgggagcag
     4681 gttaaaaaga gtattgacaa aacaaactgc agtttggaga agcttgtact ttgggaaaca
     4741 ccgaccagtt ttattaccat tgaccgaaag gatatggagt aattcaaagg gaggaaggca
     4801 tgaaggtaaa cgagattttt ttgagtatac agggtgaaag cttgtccaca ggatttccca
     4861 caatatttgt acggttttcc ggctgcaacc ttcggtgcag cttttgtgac acaacctatg
     4921 cctatgactc cggcagggat atgaccccta ataatatata tgaagaaata aaaaagcttc
     4981 attataaaag ggtatgtctt acgggagggg aacctttgat gcaagaggac ttaagtgagc
     5041 ttttggtcct tcttgaagat tatattatta ccattgagac caacgggtct ttggaattaa
     5101 agaaagtgaa gcttgtcagc ccgaaacatt cttatgttat ggatatgaag gtaccgtctt
     5161 ccggttgttc cgacaggatg gtgtttagta attttgacga gctcagggaa caggatgaga
     5221 ttaagtttgt gataggcgac aggaatgact atgaatggtc aaaagacgtt atatcaaggt
     5281 tccataacaa aggagttatt accttttcac cggtgtacgg gaagattgat tattccgaag
     5341 ttgtcaggtg gatattggac gacaggcttg atgtgaggtt tcaggttcag cttcacaaga
     5401 ttatatgggg accttataaa acaggagtat agttgacatg tataattggg aagaattaat
     5461 agaggcttgc gaaaactgta ataagtgtga actgggcaag acaaggaaga atattgttat
     5521 cggccgtggg aacagaaatg ccccgatgat gctggtggga gagggaccgg gagagcagga
     5581 ggatattcag gggcttcctt ttgtaggagc ggcaggaaag cttttggact tgctgctaaa
     5641 ggggatgaga ataccggaag acatgtatta tatagcaaat attgtcaagt gcagaccccc
     5701 atacaaccgc gttcctaccg atatagaagc agaaacctgt ttaccctact tgagaaacca
     5761 ggtgttctta ataaagccga agataatagt ctgtttgggt gctacggcta tgaagtatat
     5821 agttgaccgg aacgcgagaa tcacacaaat ccggggacag tggatcgaaa gaaagggata
     5881 ttggattatg cccacctttc atccggcggc attgctcagg gacgagtcga agaagatttt
     5941 aatgtggcag gattttaaaa gagttaagca gaggttggat gatatatgtc taaatcagag
     6001 cagttaaaga atttgtatga gttgtatgag aaagtgttct atgggaaaga aatagttttt
     6061 ggtgacggaa atcccgatac acggttaatt ttgattggag aggcaccggg aaaggacgag
     6121 gttcagcagg gaaagccctt tgtcggagca gcggggaaaa atctttcaga atttttagac
     6181 agactgggaa ttattagaga ggatatatat ataaccaatg caataaagta caggctttcg
     6241 aaagtgaatc ccgagacaaa ccgaataacc aacagaccgg ctactaaaaa agagattgag
     6301 gaaaaccggg aatacttgtt aaaggagatt gatgtcatca attccgagta tatcgtcact
     6361 ttaggaaatg ttccgttaag ggcgatatgc ggaaacaatg ttacaatcgg tgagttgcac
     6421 ggtagaaaaa acaagattga agtattggga aacgaatatg ttttatttcc gctgtatcat
     6481 cccgcaagtg ttatttataa tgtaaggctt aaagaggttt attatgaaga tttggagaag
     6541 ttaaagaaga taattgaata aaatttgcga taattaagaa aaatacataa taataaaagt
     6601 ttatatactc ttgacttaaa tatatttatg gtgatataat caaaaagctg tcgtattcgg
     6661 aggttttgat tcagagataa accgagaata aaacaaaaaa atgtcaataa aaaagtgttg
     6721 acaaagttga gacagcgtga taatatataa aagtcgcctt gacggaggca gtcaaatgct
     6781 tcagacaggc ggatatggac tttgaaaagt gaacagtgta ag
//
LOCUS       DAOOJC010000079         6749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_9389_length_6749_cov_3.570362, whole genome shotgun sequence.
ACCESSION   DAOOJC010000079 DAOOJC010000000
VERSION     DAOOJC010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9389_length_6749_cov_3.570362"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLA01_03435"
     CDS             complement(<1..299)
                     /locus_tag="PLA01_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPD00384.1"
                     /translation="MISAGDFRNGVTFELDGQIFQVVEFQHVKPGKGAAFVRTKLKNI
                     VTGATIERTFNPTDKMPKAHIERKDMQYLYNDGDLYYFMDVESYEQLPLGKDKIG"
     gene            complement(472..1551)
                     /locus_tag="PLA01_03440"
     CDS             complement(472..1551)
                     /locus_tag="PLA01_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPD00385.1"
                     /translation="MENLIEKRIEKLRLMLKEKEIDAALVTKRENYVYLSGFNGSFAY
                     LIITQKDAVLLTDFRYVQQAAKQSPLYEIVEYKDSILVTINNIVNTKGIEILGFEEDH
                     TTYKKYREFEEELNVKIIKPLEGLIDGLRMRKDHRELELIKKAVKIADDAFSHVLGYI
                     KPGVKEVEVAAELEYYMKKQGARGTSFDTIVASGYRSALPHGVASEKVIEHGEAVTMD
                     FGAIFEDYCSDMTRTVFVGKPDEELSRIYSIVLSAQQTAVRGAKKGLSGKEIDSIARD
                     IISNNGYKDYFGHGLGHGVGLEIHEEPRLSPRGSVIMDDGMVVTVEPGIYIPGLGGVR
                     IEDMIVINGNNPSVLTSSPRDMIVI"
     gene            complement(1584..2012)
                     /gene="aroQ"
                     /locus_tag="PLA01_03445"
     CDS             complement(1584..2012)
                     /gene="aroQ"
                     /locus_tag="PLA01_03445"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPD00386.1"
                     /translation="MKKVLVINGPNLNLLGQREKEIYGKETLEDIARKMNAEAMKLNI
                     DLSFIQTNHEGEIIDKIHESKGKIDNIIINPGAYTHYSIAIRDAIKAVEIPAIEVHLS
                     NIHAREEFRSKSVIAPVCAGQISGFGSMGYILALNAAASL"
     gene            complement(2213..3073)
                     /locus_tag="PLA01_03450"
     CDS             complement(2213..3073)
                     /locus_tag="PLA01_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPD00387.1"
                     /translation="MTRFGPSGNPESFYEQGYKSSDQMPAWLSAMGLEAYEYQCNRGV
                     NISEKKAREIGEEAVKNDIFLSIHAPYYINLASSEKEKRTNSKRYIIQTLTAAKWMGA
                     KRIVVHTGSYSKVNKKWALETAIGLIGEILEEADSSGLGDVLICPEVLGKNNQLGSLD
                     EIIEMCRIHERLMPTVDFGHIHARCQGSLNSPEDFERVIDMLEDALGFERIKNLHCHF
                     SRVEFTKGGEKKHWNIDDIKFGPEFAYLAEVILKKGMEPVIVCESRNDMAKDALKLKK
                     IYESLEASYR"
     gene            complement(3250..3969)
                     /locus_tag="PLA01_03455"
     CDS             complement(3250..3969)
                     /locus_tag="PLA01_03455"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPD00388.1"
                     /translation="MKKSITVGVIILLSLLITGGIMAKPYLRSVYYRIRGQKTVDDVI
                     NSIENDVKNKLDPEFNRAGVSFPPKRITFIALKQERELELWVDENNEWVYVKTYPILG
                     LSGKSGPKLKEGDRQVPEGIYKIAGLNPNSYYHLAMLISYPNDFDKEKAKKDNRTNLG
                     GDICIHGSNASAGCLAMGDEASEELFYLAVKGGIDNVKVIILPYDFRKEKNKIETEDN
                     NLEWLPELYDIINDELKEYKN"
     gene            complement(4203..6383)
                     /locus_tag="PLA01_03460"
     CDS             complement(4203..6383)
                     /locus_tag="PLA01_03460"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030248 - cellulose binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose binding domain-containing protein"
                     /protein_id="HPD00389.1"
                     /translation="MKTLICAVTILVVLAAQLLLPVPAVQRWVAAASPELNPGLRVQF
                     YNALRNDTSNTINAMFRIVNEGGAPIELDKVKLRYYYTIDKPKSQQYACDWSHVGSGN
                     VEVSIWKMVQLADRADYYFEVGFKSSAGVLEPGKSIEVQLRVWKSDWSNYQQTNDYSF
                     NVSASSYIEWERVTGYIDGVLVWGTDPEPSPVTPGPTQTPGLCVQFYNSSRNDTSNQI
                     YAMFRIVNEGDEPIELDKVKIRYYYTIDGEKSQQYACDWSHVGRENVEVSIWKMVKTI
                     DSADYYFEVGFKSSAGVLEPWESIDVQIRIWKSDWSNYQQSNDYSFNGSANDYTEWEK
                     VTGYIDGVLVWGIDTVPSTPTPSPTPTATVTPTATATVTLSPTPTPTQIPVYTPMPAD
                     GEGLRGEYYNNKDFTGISLIRIDPTVNFDWGGQAPNTNMESDTFSVRWIGEVLPKYSE
                     EYTFYTVSDDGVRLWVDNVLIIDDWTDHSATENSGKISLFGGKKYDIRMEFYENDANA
                     VAKLYWSSDSQTKEIIPKNRLFPPKMFSVVDHSVRSNGNNFVVGDYIPLQIKCNIMRP
                     VSNPVIDIDLSVKKPDGSDSGFLLKEIISGTEINKNYIKVYRNSEELAVLDYTAACVE
                     AGLGNRRLRIRVLKDFSAGEQLEVRYFVKASAKDEVFSQGIDRYLELSGLKNAVLKIN
                     FTISEWYDSGLKISTPYTADSTKDSSEKQAFRADIVIKDPVILE"
BASE COUNT         1794 a         1650 c         1039 g         2266 t
ORIGIN      
        1 tctcctatct tatccttgcc taaaggcaac tgttcatagg attcaacatc cataaagtaa
       61 tataagtctc catcgttata caggtactgc atgtcctttc tttctatatg agctttcggc
      121 attttatccg taggattaaa ggttctttca atagtagcac ctgttactat gtttttgagt
      181 tttgtcctga caaaagcagc tcccttaccc ggtttaacgt gctggaactc aacaacttga
      241 aatatctgcc catcaagttc aaaagtcaca ccatttctaa aatctccagc tgaaatcatt
      301 taattatccc tccaaaaaaa atttacattt aataacataa gttacaggta ctctgattga
      361 cctgcatatc caatattaat tcaacaagtt tatattaaaa taaaattgca actatttcaa
      421 gatatatttt cttaaacagt aaatttaatc attaatttta gctataatta cctaaatcac
      481 tatcatgtcc ctgggtgatg atgttaacac tgatggatta tttccattta ttacaatcat
      541 gtcttctatt ctcacaccac caagaccggg aatataaatg ccgggttcca ccgtaacaac
      601 cataccgtca tccattataa cacttcccct tggtgaaagc cttggttctt catgaatctc
      661 caagcctacc ccatgaccca ggccatgccc gaaataatct ttgtaaccgt tatttgaaat
      721 aatatccctc gctattgaat caatctcctt accggataaa ccttttttag ctccccggac
      781 ggcagtttgc tgtgccgaca gcacaatact atagatcctt gaaagctctt catcgggctt
      841 cccgacaaaa acagtcctgg tcatatcgga acaatagtct tcgaatattg ctccaaaatc
      901 cattgtgaca gcctctccat gctcaatcac tttttctgaa gcaacaccgt ggggaagagc
      961 tgaacggtac ccggatgcca caatggtatc aaaggacgta ccccttgccc cctgtttttt
     1021 catatagtat tccaattcag cagccacctc cacctccttt actcccggct ttatataccc
     1081 tagtacatga ctgaaggcat catcggctat ttttaccgct ttttttatta attccagttc
     1141 cctgtgatcc tttctcatcc taagtccatc aattaaccct tccaaaggct ttattatttt
     1201 aacattgagt tcttcctcaa attccctgta ttttttatag gttgtatggt cttcttcaaa
     1261 acccagtatt tcaatacctt ttgtattaac aatattgttt attgtaacca aaatgctgtc
     1321 tttgtactca actatttcat atagaggcga ctgcttcgcc gcctgctgca catatctgaa
     1381 atccgtcaaa aggacagcat ccttttgtgt aattatcaga tatgcaaagc ttccgttaaa
     1441 tcccgataga tatacatagt tttccctttt cgttaccaat gccgcatcta tttctttttc
     1501 tttaagcatt agccttagtt tttcaattct tttttcaatt agattctcca tttaatatcc
     1561 gatcctttct ctttgccgag ccactataaa ctagccgctg cattgagtgc aagtatgtag
     1621 cccatacttc cgaatccgct gatttgcccg gcacacacag gagctatcac agatttgctc
     1681 ctgaattcct cccgggcatg tatattggac agatgaacct caattgcggg aatttcaaca
     1741 gcctttatgg catcccttat tgcaatgctg taatgagtat atgcacccgg atttataatt
     1801 atattatcta ttttcccctt tgattcatgg atcttgtcaa ttatttcacc ctcatgattg
     1861 gtctgtataa agctcaaatc aatattcagc ttcattgctt cagcattcat ttttctggca
     1921 atatcctcaa gggtttcctt cccgtaaatt tccttctccc tttgccccaa tagattcaag
     1981 tttgggccat tgattacaag cacctttttc atatttattc cccctttttc attattcgaa
     2041 ggcagcaaag ttttcactct actattttac ctctccaacc tgcttgttat ttatatcagt
     2101 gtaaatttgt cggcaatacc tatttcttta cagccattat ttattccttc tgtgattaaa
     2161 ataatctcta aatagtcttt tgcagcgaca agctgaaatt ttcggtaaca ttttatcggt
     2221 aagatgcctc caaactctca tatatttttt taagctttaa ggcatccttc gccatgtcat
     2281 ttctggactc acaaacaatg accggctcca tacctttttt taaaatgact tctgccagat
     2341 atgcaaattc gggaccgaac tttatatcat caatattcca gtgcttcttt tctcctccct
     2401 tggtaaactc aacacggctg aaatggcaat gaaggttttt gattctttca aaaccgaggg
     2461 catcttcgag catatcaatc accctttcaa aatcttcagg agaattgagg cttccctggc
     2521 atcttgcatg tatatgtcca aaatccactg taggcatcag cctttcgtga atcctgcaca
     2581 tttcaattat ctcatctaaa gaaccgagct gattgttttt tcccaaaacc tcgggacaga
     2641 ttaatacgtc tccaaggcca ctgctgtccg cctcctccaa aatctcaccg attaaaccga
     2701 ttgctgtttc aagtgcccac tttttattaa ccttgctgta agaaccggta tggacaacaa
     2761 tcctttttgc acccatccat ttggctgctg tcagagtctg gataatgtaa cgtttggagt
     2821 tcgtcctttt ttctttttcg gaacttgcca gatttatata atacggggca tgtatgctta
     2881 agaatatgtc gttctttaca gcttcctctc cgatttcacg agccttcttt tcactgatgt
     2941 tgactcccct gttacactgg tattcatatg cctcaagccc cattgccgaa agccatgccg
     3001 gcatctggtc agaagacttg tatccctgct cataaaaact ttccgggtta ccggaaggtc
     3061 caaaccttgt caatacaatc acctcataaa aacttctctt tgtccataac ttccctgctt
     3121 taagggaata aagcatttat attattattt ctaatgttcc tctgttttaa aaggatacat
     3181 gttatttaag caatataaaa tgtataaaaa acatataaaa gttttctcta aacaatcttt
     3241 agcaaagtgc taatttttat actctttcaa ttcgtcgtta ataatatcat acagttccgg
     3301 cagccattcc aaattattat cttctgtttc aattttattt ttttcttttc taaagtcata
     3361 aggcaatatt attaccttta cgttatcaat cccgcctttt actgccaggt aaaaaagttc
     3421 ttcacttgct tcatctccca tggctaaaca cccggctgaa gcattgcttc catgtataca
     3481 tatatctccg cccagattgg tgcggttatc cttctttgct ttttccttgt caaaatcgtt
     3541 gggataactt atcagcatag ctaaatgata ataactgtta gggtttaatc cggcaatttt
     3601 atagatacct tccggaacct gcctgtcacc ttcttttagt tttggaccgc tttttccgct
     3661 cagtccaagg ataggatatg tctttacata cacccattca ttgttttcat caacccacaa
     3721 ttccaattct ctttcctgct ttaaagcaat aaatgttatt ctttttggcg gaaaactgac
     3781 tcccgccctg ttaaattcag gatcaagctt gttcttaacg tcattttcaa tactatttat
     3841 cacatcatca acagtttttt gacctcttat acggtaataa accgacctta aatagggttt
     3901 agccataata ccgcctgtga taagcagcga aaggagtata ataactccta ctgtgattga
     3961 tttcttcatt acatcatacc tgcccattca tgccttttta acagacaata aattataaaa
     4021 tatgaatcca tatttatcat aaatttaatc catattttat cacattatat tttatcctgt
     4081 ccaatattcg ttaccctttt caatattttg aatagtattg gaaatgagaa caaagaaatt
     4141 gggcttttag tccttaaact aattataacc ccggtaacgg gtgcattaaa tatcatgtat
     4201 cgtcactcaa gtattaccgg atcctttata actatatctg cccgaaatgc ttgtttctca
     4261 cttgaatcct ttgttgagtc cgcggtatag ggtgtgctta ttttcaaacc actgtcatac
     4321 cattcactaa tcgtaaagtt tatcttcaaa accgcattct taagcccgct taattccagg
     4381 tacctgtcaa ttccctgact gaatacctcg tctttagccg atgcctttac aaaatacctc
     4441 acctcgagct gttctcccgc agaaaaatct tttaataccc ttatcctgag cctgcggttt
     4501 cccaaaccgg cctcaacgca tgcggccgta taatctaaaa ccgccaattc ttccgaattt
     4561 ctgtagactt ttatgtaatt cttgttaatc tccgtcccgg aaattatctc ctttagcaaa
     4621 aaaccgctgt cgcttccgtc cggttttttc accgaaaggt caatatcaat aaccggattc
     4681 gaaaccggcc tcataatatt acattttatt tgcagaggta tgtaatcccc gacaacaaaa
     4741 ttgttcccgt tcgaccgtac gctatggtca acaaccgaaa acatcttcgg cggaaacaat
     4801 cggttcttcg gtatgatttc ctttgtctgg ctgtcgctcg accaataaag ctttgccacc
     4861 gcattagcat cattttcata aaattccatt ctgatatcgt atttttttcc tccgaaaagg
     4921 cttattttac cgctgttttc cgttgcagag tgatcggtcc agtcatcgat tatcagaacg
     4981 ttatcgaccc acagtctcac cccgtcatcg gacacggtat aaaaagtata ttcctctgaa
     5041 tacttgggca gaacttctcc tatccatcgg acggaaaagg tgtcgctttc catgttcgta
     5101 ttcggtgcct gaccgcccca atcaaagttc accgttgggt caattcttat cagggatatg
     5161 cccgtaaagt ctttgttgtt gtaatactct ccccttagcc cctccccatc ggcaggcatg
     5221 ggtgtataaa ccggtatttg agtcggtgta ggtgtaggtg acaaagtcac agtagcagta
     5281 gcagtcggtg tcacagtcgc agtcggtgtc gggcttggcg taggtgtaga aggcacagta
     5341 tctattcccc aaaccaatac accgtcaata tagcccgtta ccttttccca ttctgtataa
     5401 tcatttgcac tgccgttaaa ggaataatca ttggactgtt gataattgct ccaatcgctt
     5461 ttccagatcc ttatctgcac atctattgat tcccatggtt ccagcacccc ggcactgctt
     5521 ttaaacccca cttcaaaata ataatccgca ctgtcgattg ttttaaccat cttccatata
     5581 ctaacctcta cattctccct gcccacatgc gaccaatcac acgcatactg ctgtgacttt
     5641 tccccgtcta ttgtgtaata atacctgatt ttcactttat caagctcaat aggttcatca
     5701 ccttcattta caattctgaa catggcataa atctggtttg atgtatcgtt cctcgacgaa
     5761 ttataaaatt gtacacaaag gcccggggtt tgtgtcggtc ccggggttac cggagaaggc
     5821 tccggatccg ttccccaaac caatacaccg tcaatatagc ccgttactct ttcccactca
     5881 atataactgc ttgcactgac gttaaaggaa tagtcattgg tctgctgata attgctccaa
     5941 tcgcttttcc agacccttaa ctgcacttct atcgattttc cgggttccag cacacctgca
     6001 ctgcttttaa accccacctc aaaataataa tctgccctgt cggccagttg gaccatcttc
     6061 catatgctta cctccacatt accactgcct acatgtgacc agtcacacgc atactgctgt
     6121 gacttcggct tgtctattgt gtaataatac cttagtttca ctttatcaag ttctataggt
     6181 gctccgcctt catttacaat tctgaacatg gcgttaatag tgtttgacgt atcattcctc
     6241 aatgcattgt aaaactgtac acgaaggccc gggttcagtt caggagaagc tgcagcaacc
     6301 catctttgta ctgcaggaac agggagcagc aattgagcag ccaaaacaac caatattgtc
     6361 accgcacaaa tcaacgtttt cataaatata acccccgtcc atcgtatgtt catagctatt
     6421 ttgcttaatg ctgcgtaagt acttttatgt ttttttgtgt cgatattctt cgatatgtac
     6481 ccttactcaa gcattgccgg aacctttata actccggagt gcctgtttct tacttgaatc
     6541 ctcttctaag ttttatggta cagggtgtac ttgtcagtct atttcatgcc acttaaccac
     6601 ttttatgttt ttcgtcgata ttctttggct ctttaaggtt tcatccgaaa tctcataggt
     6661 taaaaggcca aagaaaccgt taatattgac atctgcgttt aaaaaaccgt ccactgcctc
     6721 ttcgtcatat ataatgtctg catttccca
//
LOCUS       DAOOJC010000080         6713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_9443_length_6713_cov_3.940072, whole genome shotgun sequence.
ACCESSION   DAOOJC010000080 DAOOJC010000000
VERSION     DAOOJC010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9443_length_6713_cov_3.940072"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLA01_03465"
     CDS             <1..310
                     /locus_tag="PLA01_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00390.1"
                     /translation="TGKGEAYLQEKAIEYTWVQGLSSKDSSALTSEQATMAVALLGVK
                     SLTLLPAFGIIAKAAILPNVGTGKGGHGRSTSGCSSCSSCSSCSSCSSCGGCGGCGGD
                     "
     gene            313..1332
                     /locus_tag="PLA01_03470"
     CDS             313..1332
                     /locus_tag="PLA01_03470"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD00391.1"
                     /translation="MNCDFSVRKDTLILQWHITSRCNLRCAHCYQESYHRPECSFTQM
                     LSVIEQYKKLLLKLNRKGHINVTGGEPFLREDFFDLLKVFNEHKDYFNFGILTNGTFI
                     DKANAKRLKLLNPSFVQVSIEGDEKTHNSIRGEGNLNKVIEAIMHLKHNGIKTLVSFT
                     AHKENYKSFPDVAALCKKIGVFKLWTDRMVPFGGAEKMQDSLLNPEETMEFFNIVKRC
                     RKQKKWGRRNKTIIELNRALQFFVNDNYPYSCNAGESLICLLEDGTVLPCRRLPIEAG
                     NLKELSLEQIYFGDVMKDLRREAPPPEGCNRCSYFILCKGGARCISYAVTGDPFARDP
                     GCPII"
     gene            complement(1453..1800)
                     /locus_tag="PLA01_03475"
     CDS             complement(1453..1800)
                     /locus_tag="PLA01_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HPD00392.1"
                     /translation="MAKYEKRFKGDFKEFLDWLHEDITNGSISASCEDTSDITIGEVK
                     VAVRVYERYSMMGSSRVSLNVTAVGQGDNIFVSAISSGGSRAVFFKINTIGEETFLDL
                     CIDSVERYISEHR"
     gene            complement(2091..3026)
                     /locus_tag="PLA01_03480"
     CDS             complement(2091..3026)
                     /locus_tag="PLA01_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HPD00393.1"
                     /translation="MQKKIPTREAYGNTLAEIGCDERIVVMDADLSKSTKTAVFKEKY
                     PERFFNMGIAEANMMSAAAGIASCGKVVFASTFAMFASGRAFEQVRNSICYPQLDVKI
                     GASHAGITVGEDGASHQTIEDIAIMRVLPNMTVVSPADAVEARFATIAAYKTPGPFYL
                     RLTRLAVPVILDENYKFELGKGVTLKEGNDVTIIATGFMVSKSLEAVDILKQEGINAR
                     LINIHTIKPIDKDIIIKAALETGAIVTCEEHSVIGGLGSAVAEVLAEYYPVRMKRVGI
                     MDKFGKSGKPEELLAMYGLTPKDIVEKVKEVLKKK"
     gene            complement(3028..3873)
                     /locus_tag="PLA01_03485"
     CDS             complement(3028..3873)
                     /locus_tag="PLA01_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPD00394.1"
                     /translation="MYEMRPNELRRYSTIIRKHIIEEVYHAASGHPGGSLSCADIITV
                     LYFDEMRVDVKNPQWEDRDRFVLSKGHCSPALYGALAEKGFFPKEELLRFRHIDSYLE
                     GHPSMRMVPGVDMSTGSLGQGISAAVGMAIAGKLDKKDYKVYVLLGDGEIQEGQVWEA
                     LMSAAHYKLNNLIAFLDHNRLQIDGNITDVMSPEPVEDKFRAFGWEVINIDGHDHEQI
                     KNAVKKAKTVEDKPIMIVADTIKGKGVSFMENNAGWHGSPPNKEQRDKAIAELDKMLA
                     EMEGK"
     gene            complement(3995..4900)
                     /locus_tag="PLA01_03490"
     CDS             complement(3995..4900)
                     /locus_tag="PLA01_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPD00395.1"
                     /translation="MNIFYDDIKNWKDYIIITLGSMITAISINAFLVPYRLAAGGVSG
                     IATVIYYLSGGKLPVGTLMLILNVPLFVLGIRFVGKQFILRTVYATIVLSVLIDITKP
                     LTEEFVKKFFMGFTEQSYAQDILLYSIFGGFLMGTGLGLVFRSRATTGGTDLAARIVN
                     HFAPAYTMGQVLLFIDVGVILFAAVSFQSFRLALYSIVALYVSSKVIDALLEGVKFAK
                     ALFIISEAPKEIASRILRELDRGVTSLKGKGMFTETDKEVLFCVIHRGQLLQIKKIVK
                     EEDKKAFIVLTDIREVFGEGFKTYE"
     gene            5265..>6713
                     /locus_tag="PLA01_03495"
     CDS             5265..>6713
                     /locus_tag="PLA01_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD00396.1"
                     /translation="MKVLHLIGGGDVGGAKSHVLSLVKELDKHIDVKLISFRTGAFAD
                     DARKMGINVEIVKTGTIISDVRKVLSIIKNEGYELIHSHGAKANMIAFFAKKFTSLPV
                     VTTVHSDYRLDYLQNILKMFSFGLINTVVLRFLDYYIGVSKNFKEMLIKRRFSPQKIF
                     TVYNGINFHQEISPLPKEEFLKKYNLNFGKDDVIVGILARLDPVKGLDTFLKAAQGVC
                     QKNPSVRFLIGGDGPERKSLEKKALSLGLKDNVFFLGFVDRPFDLINCLDINTLTSLS
                     ESFPYAILEGSLFKKATVSSNVGGISDLIENGKNGFLFEPGDYKSLADILLTLVKDSS
                     LRKEMGEKIYEKASTYFSLDNMCKTQLEIYQNILAQRANKSKYRYDAIISGYYGFKNI
                     GDDAMLMAIIDNMKMYRKDMRILVLSKNPIETSLIYNVDSINRFNLLKILFTMKKSKV
                     FINGGGSLIQDNTSTRSLVYYLAMIWLAKKQGM"
BASE COUNT         1966 a         1501 c         1269 g         1977 t
ORIGIN      
        1 caccggtaaa ggtgaggcat atttgcagga aaaagccatt gagtacacat gggtccaggg
       61 cctttcaagc aaggattctt cagctttgac cagtgaacag gctactatgg cagttgctct
      121 acttggtgtt aaaagtttaa ctctattacc tgccttcgga atcatagcaa aagccgcaat
      181 tttacccaat gtgggtaccg gcaagggagg tcatggtcgt agtacttcgg ggtgcagcag
      241 ctgcagcagc tgcagcagtt gtagcagttg cagcagttgc ggcggctgcg gtggttgcgg
      301 aggagattag atttgaattg tgatttttca gtcagaaaag acacgttaat ccttcaatgg
      361 catataactt cccgatgtaa tttaagatgt gcccattgtt accaggaaag ttaccatcgg
      421 ccggaatgca gttttaccca gatgctttct gttattgagc agtataagaa gctgcttttg
      481 aaactgaaca gaaaagggca tatcaacgtt acagggggag aaccctttct tcgcgaggat
      541 ttctttgatt tactaaaggt ttttaatgaa cacaaagatt attttaactt tggaatactg
      601 accaacggaa cttttatcga taaagccaat gccaaaaggt taaagcttct gaatccttcc
      661 tttgttcagg taagcatcga aggggacgaa aaaacccaca actccatccg cggggaggga
      721 aacctcaata aggtaattga agccataatg catttaaaac ataacgggat taaaaccctg
      781 gtgtctttta ccgctcataa agaaaactac aagagttttc ccgatgttgc cgctctgtgt
      841 aaaaagatag gggtatttaa actctggact gaccgcatgg taccctttgg aggtgcagaa
      901 aaaatgcagg acagtttgtt gaatccagag gaaaccatgg aattctttaa tattgtcaaa
      961 aggtgcagga agcagaaaaa atggggaaga agaaataaaa cgatcataga gcttaacagg
     1021 gctttgcagt tttttgtcaa cgacaactat ccctattcct gtaatgcagg cgagtctttg
     1081 atatgcttat tggaagacgg tacggtgtta ccctgcagaa ggctgcctat agaagccgga
     1141 aatctaaaag agttgtcttt ggaacaaata tatttcggcg atgtaatgaa agatttaaga
     1201 agggaagcac ctcctccgga aggatgcaac cggtgctcat atttcatatt atgcaagggc
     1261 ggggcccggt gtatttctta cgccgtaaca ggggatccct ttgcccggga cccgggatgt
     1321 ccgattattt agcctctccc tgatagccaa tatatgacct cattctcata ataacatata
     1381 ccaaaaccgc atcggtcatg gtcgtcatca taaagataag gccaactgca gccagaagag
     1441 tccaaagtcc ttttaccggt gttcggaaat gtatctttcc actgaatcta tgcaaagatc
     1501 aagaaaggtc tcttctccaa tcgtattaat tttgaaaaaa accgctcggc tcccgccgga
     1561 agaaatggca gaaacaaata tattatctcc ctgacccaca gcggtaacat taagactgac
     1621 acgactactt cccatcatgc tataccgctc ataaacccga acagccacct tgacttctcc
     1681 tatagtgatg tcgcttgtat cctcacaact agcggaaatg ctcccattag taatgtcttc
     1741 atgtaaccaa tctaaaaact ctttaaaatc tcccttgaat cttttctcat atttggccat
     1801 gtttttatac tccttttaaa atctcaacga atatctcatt ttgattccac tatcatttta
     1861 tcagattgta catgcctgcg tccaattatt tgcaactgat ttcaaccgct atcaaaaact
     1921 cctgcaaagt agcatatatg ctactttgca ggagtcatta actttaacgt ccaaatgtga
     1981 agaaaagtgt tccagaaact taagttacgc aagtcagagc ctgtaaatac tccgtggggg
     2041 tcgagcaaaa ctcacttcgt gaaatcttac ttaatcttaa accaagcctg ctacttcttt
     2101 ttcaatactt ccttgacctt ttcaacaata tccttcggtg tcaatccata catcgccaat
     2161 agctcttccg gcttgccgga cttgccgaac ttatccatta ttccgaccct cttcatccgg
     2221 acgggataat attcggctaa aacctcagct accgcactgc ccagtcctcc gatgacactg
     2281 tgctcttcac aggtaacaat cgcaccggtc tcaagtgcag cctttattat tatgtctttg
     2341 tcaatcggct ttattgtatg tatgttaatc aagcgggcat taattccctc ctgctttaga
     2401 atatccacag cctccaaaga ttttgacacc ataaatcctg tggcaattat tgttacatca
     2461 ttgccctcct tcagggtaac tcccttgccc agctcaaact tatagttttc gtcaagaatc
     2521 accggcaccg caagcctcgt aagtctcaaa taaaagggtc ccggagtctt gtatgcggca
     2581 atggtagcaa accttgcttc tacagcatcc gccggactga ctacagtcat attgggtaaa
     2641 accctcatta tagctatgtc ctctatggtc tgatgagatg caccgtcttc ccctaccgtt
     2701 attccggcat gggatgcacc tatttttaca tccagctgag ggtaacagat ggaatttctg
     2761 acctgttcaa atgcccttcc cgatgcaaac attgcaaagg tacttgcaaa tactactttt
     2821 ccacaggatg caatacctgc agccgccgac atcatattgg cttctgctat ccccatattg
     2881 aagaacctct cagggtattt ttctttaaaa actgcggttt ttgtagactt tgagagatcc
     2941 gcatccataa ccactattct ttcatcgcag cctatctctg ccaaagtatt gccataagct
     3001 tcccttgtcg gtattttttt ctgcatgcta tttcccctcc atttctgcca gcatcttatc
     3061 caactcggca atagccttgt cccgctgttc tttgtttgga ggtgatccgt gccaacctgc
     3121 gttattctcc ataaaggata ctcctttgcc ctttatagta tccgcaacta tcataatcgg
     3181 cttatcctct accgtttttg ccttctttac cgcatttttt atttgctcgt ggtcatgtcc
     3241 gtcaatattt atgacctccc agccgaaagc cctgaattta tcttccacag gctcgggaga
     3301 catgacatct gtaatattgc cgtctatctg caaacgattg tgatccagaa aagctataag
     3361 gttattgagc ttgtaatggg cagcggacat taatgcttcc caaacctggc cttcttgtat
     3421 ttcaccatcg cctaaaagta catatacctt gtagtctttt ttatccagct taccggcaat
     3481 agccatacct actgcggctg atattccttg tcccagtgaa ccggttgaca tatccactcc
     3541 cggtaccatt ctcatactgg gatgtccctc aagataactg tctatatgtc tgaacctaag
     3601 aagctcttcc ttaggaaaaa agcctttctc cgccagggca ccatagagtg caggagaaca
     3661 atgccctttt gacaatacaa acctgtctct gtcttcccat tggggatttt tcacatctac
     3721 tctcatctcg tcaaaatata aaacggttat aatatccgca catgatagtg acccgcccgg
     3781 atgtcccgat gctgcatgat aaacctcttc gataatgtgc ttcctgataa ttgtagaata
     3841 tcttctaagc tcatttggtc tcatctcgta catttttaca actcctgaat tttatgtttt
     3901 tagtatagtc tagtatacca tagtaaaacc atataagtcc atagttttaa attcaattta
     3961 acgtaaattg aatttaatat ttgtcaagct ttactcactc atatgtttta aagccctctc
     4021 cgaaaacctc tcttatatcg gtcagtacaa taaacgcctt tttatcctct tcttttacaa
     4081 ttttctttat ttgcaggagt tgtcccctgt gtatgacaca aaaaagtacc tctttatcgg
     4141 tttctgtaaa catgcctttg cctttcagcg atgtaacccc gcggtctaac tccctcaaaa
     4201 tcctcgatgc aatttccttt ggggcctccg atattataaa cagggccttt gcaaatttca
     4261 ctccctccaa aagggcatca attacttttg aactgacata cagtgcgaca attgaatata
     4321 atgcaagccg gaagctctgg aacgaaaccg ctgcaaaaag tattactcca acatctataa
     4381 agagcaatac ctgacccatt gtataagccg gtgcaaaatg gtttactatt cttgcggcaa
     4441 gatccgttcc cccggttgta gcccgtgatc tgaataccag gcccaatccg gttcccatca
     4501 aaaaaccgcc gaatatcgaa tagaggagaa tgtcttgagc ataggactgt tccgtaaacc
     4561 ccataaagaa ttttttaaca aattcttcgg ttaacggctt tgtgatatct atcagcacgg
     4621 agagtacaat tgtggcatac accgttctta aaataaactg ttttccgacg aaccgaattc
     4681 ccaaaacaaa cagcggtaca ttcaatataa gcattaatgt ccccaccggc agttttccgc
     4741 ccgacaggta atagataact gtggctattc cgctgactcc gccggctgct aacctgtatg
     4801 gaaccaaaaa agcattaatt gatattgcgg ttatcatcga acctaaagtt ataataatgt
     4861 aatctttcca gtttttaatg tcatcgtaaa aaatattcat atattccctc gacccgtttt
     4921 ttctatagaa gcttatcaag tatcaagctt ccatgtactt tgcagtttta gttttgctat
     4981 ttcccgaata agtctattgt taagtgtttt ttcaaaacac attctatagt ttatattgcc
     5041 cataaaaaag caagttaatg ttttaaaaaa tgctgtgtac ttacgaaaca taaattttag
     5101 aatatttttt tcgcattttt attccatgca cacaagccgg ctttacatac tttcatttaa
     5161 accacataat atgctataat gtgatggatg cgggacaaac taatatatgg ttgcgtccac
     5221 aaaattgcat tgaataatta aatgactaat cgaggagagt attcatgaag gttcttcact
     5281 taattggcgg aggggatgtt ggcggtgcaa aaagccacgt actttccctt gtaaaagaac
     5341 tggacaaaca catagatgta aagctcatca gcttcaggac cggtgccttt gccgatgatg
     5401 cacgaaaaat gggcattaac gttgaaatcg taaagacagg gaccattata tcggatgtcc
     5461 gcaaggttct gagcatcata aaaaatgaag ggtatgagct tattcattcc catggtgcga
     5521 aggccaacat gattgccttt ttcgctaaaa agtttacatc tcttcctgtg gttacaacgg
     5581 ttcacagtga ttacaggctt gattaccttc agaacatatt gaaaatgttc tccttcgggc
     5641 ttataaacac cgttgtcctg aggtttcttg actattacat aggcgtttcg aaaaatttta
     5701 aggaaatgct tataaaaagg cgattcagtc ctcagaaaat atttacggtt tacaacggaa
     5761 tcaatttcca ccaggaaata tcccctcttc caaaagagga gttcttaaaa aaatacaacc
     5821 tgaactttgg aaaagatgat gtgattgtcg gtatattggc aaggcttgat cccgttaaag
     5881 gattggatac ttttttaaaa gctgcccaag gagtttgtca gaaaaatcct tctgtaaggt
     5941 ttttaatagg aggagacggt ccggaaagaa aatcccttga aaaaaaggcc ttgtccctgg
     6001 gacttaaaga caatgttttc ttccttggtt ttgtcgaccg gccttttgat ttgataaact
     6061 gcctggacat aaacaccctt acatcattga gtgaaagctt tccctacgct atactggagg
     6121 gctccctttt taagaaggct accgtaagca gcaatgtcgg aggaatttcc gacttgattg
     6181 aaaacggaaa aaacggtttt ctctttgagc cgggagatta taagtccctg gccgacatac
     6241 tgcttaccct tgtaaaggat tcttccttga gaaaggaaat gggagaaaaa atatatgaaa
     6301 aggccagcac atatttttct ttggacaaca tgtgcaaaac ccagcttgaa atatatcaaa
     6361 acatactggc acaaagggca aataaaagca aataccgcta cgatgccatt atttccgggt
     6421 attatggctt taaaaatatc ggagatgatg ccatgctcat ggcaataata gataacatga
     6481 aaatgtacag gaaagatatg agaatattgg ttttatccaa aaacccaata gaaaccagtt
     6541 tgatatacaa tgttgattcg ataaacagat tcaaccttct aaaaatacta ttcacaatga
     6601 aaaaatcaaa ggtattcata aacggcggcg gaagccttat acaggacaat accagcaccc
     6661 gttcattggt gtactatctt gcgatgatat ggctcgcaaa aaagcagggc atg
//
LOCUS       DAOOJC010000081         6625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_9597_length_6625_cov_4.532877, whole genome shotgun sequence.
ACCESSION   DAOOJC010000081 DAOOJC010000000
VERSION     DAOOJC010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9597_length_6625_cov_4.532877"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4566)
                     /locus_tag="PLA01_03500"
     CDS             complement(<1..4566)
                     /locus_tag="PLA01_03500"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPD00397.1"
                     /translation="MKRIAFIDTEIDPKSGKILDIGSIKDNGASFHSGSVAAFIEFLQ
                     GSEYVCGHNIIRHDLKYIKNAVEAAGINNSNSIDTLFLSPLLFPKKPYHSLVKDDKLQ
                     TEDINNPLNDSIKARDLFFDEINAFYELDNELKRIFYTLLRDKGGFSAFFKYIDATSK
                     SGMENNPVVPKTFVHLAGYELELRDLEKTIRNKFRFQICQKADLGKIISQNPIELSYC
                     LALINTRCHYSITPPWVLKTFPEVERVMFLLRNNPCLEGCDYCNEALDIHKALKKYFG
                     YDSYRTYDGEPLQEKAVKAAVANKSLLAVFPTGGGKSVTFQVPALMSGMNSKGLTVVI
                     SPLQSLMKDQVDNLEKAGITDAVTINGLLDPIERAKSFERVENGSASLLYISPESLRS
                     RSIERLLLGRNVVRFVIDEAHCFSAWGQDFRVDYLYIGDFIKLIQEKKNMTEPIPVSC
                     FTATAKPNVIEDIRDYFKEKLSIDMELFTSASTRHNLRYKVFDRKSEEEKYNTLRDLL
                     EENDCPTIVYAVTTNKVYNLAQRLIQDGFSARAFHGKMDKDEKTENQDAFIKGDVRIM
                     VATSAFGMGVDKKDVGMVIHYEISDSLENYVQEAGRAGRDENITADCYVLFNEEDLNN
                     HFMLLNQTKLNIEEIQQVWRAIKDVTRLRMKASQSALEIAKRAGWNEGLDDMETRVKA
                     AIAALEDAGYIKRGQNMPRVFANSILARNVHEAVDKINASHRFGDKQKQNAIRIIKSL
                     ISSKSRKIAMDEDAESRVDYISDHLGIPREGVIQIVNLLREEGILADMKDLVAFIKKN
                     QNRSLGILKSFANIENFLLSVFSKEEKTFHIKELNEQAEQYGCKDSTPNSLKTVINLW
                     NKKGWIKRQNTGFSANHIAVITLFDKRLLEEKIIKRHELSRFILEYLYENTKYGIQEN
                     DAKEEVEVEFSVLELKEKFEQRITFFNTKVSSDDVEDALYYLSKIGTLKIEGGFMVIY
                     NPMSIERIERDNKKRYKVEDYKKLKAFYENKIQQIHIVGEYALRMISDYKDALQFVDD
                     YFTLNYSSFLNKYFRGERQDEIKRNITNEKFRQLFGELSAAQLKIITDKQSKNIVVAA
                     GPGSGKTRVLVHKLASLMQMEDVKHEQMLMVTFSRAAATEFKKRLIKLIGNAANYIEI
                     KTFHSYCFDLIGRVGTLEKSDEVIREAVNRIKNGEVEPGRITKTVLVIDEAQDMDADE
                     FELISTLMERNEDMRVIAVGDDDQNIYEFRGASSKYLEQMIKEKNAVMYELIENYRSK
                     SNLVEFTNWFVQRISHRLKNMPIVAVQRDNGRIKLVRYKSGNLITPVVNDILNAQLSG
                     TTCVLTISNEEALQVTGLLVKNGMQAKLIQSNEGFNLYNLLEVRSFLKYLNLSKDTYL
                     IGEETWAEAKRKLAYEFYRSPNLELCLNIIKDFEATNPKSKYKSDLEIFIRESRMEDF
                     FTEKLETIFVSTIHKSKGREFDNVFIMLDGFNPKADEELRQLYVAMTRAKRSLTLHYN
                     GNYLDFVRADGLETFYDSNIYRPPGQLA"
     gene            4844..5332
                     /locus_tag="PLA01_03505"
     CDS             4844..5332
                     /locus_tag="PLA01_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6323 family protein"
                     /protein_id="HPD00398.1"
                     /translation="MSFEIMNTSSSLIQKQILEEIKKCNDYTAQFGLTLSHSDALELI
                     ETCNHSLKRTGRIEFGGSIVSKIIMEFCDSPYISMHSYAQTIHELTEIFYLYKNETLD
                     LISDDALIKFMKKCFDGKCQGSLELLSERELYNMAHNLRYGLAPDYSEDSNTDEEDEE
                     NE"
     gene            5307..>6625
                     /locus_tag="PLA01_03510"
     CDS             5307..>6625
                     /locus_tag="PLA01_03510"
                     /inference="COORDINATES: protein motif:HMM:NF040016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6179 domain-containing protein"
                     /protein_id="HPD00399.1"
                     /translation="MRKMRKMSNLEKQHMINTNNTNNLRNEAYFASLLKEAYSQGILN
                     DLEAENIQLQCINFLAKKCEKFNDGMSSSIRVEIAERIMESNLYTIGLYLKSLPDVQC
                     AINELKTLLISEMYEKGKKLINTKFASTNHLYKMVQKNKLTTINYSYNATLSDSGIGS
                     FFKLYNPDYGAHDCASSVDYQLCNPITDLVGVEYIQKYLESLYLENKFCRNFAAEYIH
                     KLLYGYYEGYKDLLINIFEHVLTAAIGCVLAKRNILGLDISQEEVQSLQNELSKDDAP
                     LIALKIQNAAAGVLEDLNIQSPSLQGYIEKSLSKITTNIIHAVKTNTLGKTFVSLVNP
                     DLKPKIQFSFGKKMDDEDFWKFIDELSECRFSSDKIAFIQQNVKSLGDLEDILLDVQL
                     GKEETYLVLDKLTDIELAALVKRHTFKSDLQAVDLSEAEETLQNNLK"
BASE COUNT         1942 a         1395 c         1170 g         2118 t
ORIGIN      
        1 tgccagttga ccggggggac ggtaaatatt cgaatcataa aaagtttcaa gtccgtcagc
       61 cctgacaaag tccaagtaat ttccattata gtgaagggta agactacgtt tagcccgtgt
      121 cattgcaaca tacagctgcc tgagttcttc atccgctttt ggattgaacc catccaacat
      181 tataaataca ttgtcaaact ctcttccctt tgacttgtga atggtcgata caaatatggt
      241 ttccagcttt tccgtgaaaa aatcctccat tcgtgattct ctgataaaga tttccaggtc
      301 cgatttatac ttactttttg gattggttgc ttcaaagtct tttattatgt ttaaacacaa
      361 ctccaaatta ggactcctgt aaaactcata tgccagcttt ctttttgcct ctgcccaggt
      421 ctcctcacct atcaaatagg tatccttgga caggtttagg tactttaaaa acgaccttac
      481 ttccagaagg ttgtataggt taaaaccttc attggactga atcagctttg cctgcatgcc
      541 gtttttgacc agtaagccgg taacctgtaa tgcttcttca ttacttatgg tcaaaacaca
      601 ggttgttccc gaaagctgtg cattaagaat atcgttaacc accggtgtaa tcaggttgcc
      661 gcttttatac cggaccagct ttattcttcc gttatccctc tgaaccgcaa caataggcat
      721 attctttaat cggtgggata ttctctgtac aaaccagttt gtaaactcca caaggttgct
      781 cttactcctg taattttcta tgagctcgta cataacggca tttttctctt ttatcatttg
      841 ttccagatac ttggagcttg caccccggaa ttcataaata ttctggtcgt catcccccac
      901 tgcaattacc ctcatatcct catttcgttc catcagggtg ctgattaatt caaactcatc
      961 ggcatccatg tcctgagctt catctataac caaaacggtc tttgtaattc tgcccggttc
     1021 cacctcaccg tttttgattc tattcacagc ctctcttatt acttcatcgg acttttccag
     1081 tgtccccacc ctccctataa ggtcaaagca ataggaatga aaggttttta tctcaatgta
     1141 gttggcggca ttgccaatga gttttataag gcgtttttta aactcagtgg ctgcagccct
     1201 cgaaaaagtt accatcagca tttgttcatg cttgacatcc tccatttgca tcagtgacgc
     1261 cagcttgtga accaataccc gggtcttacc gcttcccgga ccggctgcaa ctacaatatt
     1321 ctttgattgt ttatcggtaa tgatttttaa ttgggcagcg gaaagctccc caaaaagctg
     1381 ccggaatttc tcattagtaa tgtttctttt tatttcatcc tgcctctccc ctctgaagta
     1441 tttatttaaa aaggagctgt aattcaacgt aaaataatca tccacaaact gaagagcatc
     1501 cttataatcg ctgatcattc ttagagcata ttcaccgaca atatgaatct gctgaatttt
     1561 gttttcgtaa aatgctttaa gctttttgta atcctctacc ttgtagcgtt ttttgttgtc
     1621 cctttcaatt cgctcaatag acatagggtt atagatgacc ataaagccgc cctctatctt
     1681 cagagtcccg atttttgaaa gataataaag tgcatcctca acatcatccg aagaaacctt
     1741 ggtattaaaa aacgttattc tttgctcaaa cttttcttta agttcaagaa ccgaaaactc
     1801 tacttccact tcctctttag cgtcgttttc ctgtataccg tacttggtat tttcataaag
     1861 atactccaat ataaaccgtg acaattcatg cctttttata atcttttctt caagcaatct
     1921 tttatcaaac aaagtaatta ccgctatgtg gtttgccgaa aagcccgtat tctgcctctt
     1981 tatccaacct tttttattcc aaaggtttat gacagttttt aaactgttgg gggttgagtc
     2041 cttgcatcca tattgttcag cttgttcgtt gagctccttt atgtgaaatg ttttttcctc
     2101 ctttgaaaaa acactcaaaa ggaagttctc aatatttgca aaggatttta gtatacccag
     2161 cgaccggttt tggtttttct taataaatgc cacgaggtct ttcatatcag caaggattcc
     2221 ctcttcccga agcaggttca caatctgaat tactccctcc cggggaattc ccaaatggtc
     2281 cgaaatatag tcaaccctgg actctgcgtc ttcatccatt gcaatttttc tacttttgct
     2341 ggaaataagg ctctttatta ttcgaattgc attttgcttt tgcttatcgc caaacctgtg
     2401 ggaagcatta attttatcga ctgcctcatg aacatttctc gcaagaatac tgttggcaaa
     2461 tacccggggc atattttggc cccgcttgat ataaccggca tcttccaaag cagctattgc
     2521 cgccttcacc ctggtttcca tatcatcaag gccctcattc cagccagccc tttttgcaat
     2581 ttccaatgcg gactgggatg ctttcatccg cagtcgtgta acgtctttaa tagccctcca
     2641 tacttgctgg atttcttcaa tattcagttt tgtctgattt agcagcataa aatgattgtt
     2701 gaggtcttct tcattgaata acacataaca gtcggcagtt atattttcat cccttccggc
     2761 ccgtcctgct tcctgaacgt aattttcaag ggaatccgat atttcgtagt gaatgaccat
     2821 tcccacatcc ttcttatcca ctcccattcc gaatgcggaa gttgcaacca tgatcctgac
     2881 atcacccttt ataaaggcat cctgattttc ggttttttca tccttatcca ttttcccatg
     2941 gaatgccctt gccgaaaatc cgtcctgtat aagcctttga gcgagattat acaccttgtt
     3001 tgtcgttacg gcatagacta ttgtggggca atcattttcc tctaacaaat cccttaatgt
     3061 attgtatttt tcttcttcac tttttctatc gaataccttg tatcgaagat tgtgccgggt
     3121 tgacgccgat gtgaaaagct ccatatcaat ggatagcttc tccttaaaat aatctcttat
     3181 atcctcaata acattgggct ttgcagtggc tgtaaagcag gaaacgggaa tgggttctgt
     3241 catgtttttc ttttcctgaa ttaactttat aaagtcaccg atatacaaat aatcaactct
     3301 aaaatcctgt ccccaagcgg aaaagcagtg ggcctcatct atgacaaacc gaacaacatt
     3361 ccttcctaat agcaaacgct caattgacct tgaacgcaag gactccggcg aaatgtaaag
     3421 aagggatgcc gaaccgtttt ccaccctttc aaaggacttt gccctttcaa tcgggtccaa
     3481 taacccgttt attgtaaccg catcggtaat gccggccttt tccaaattat ccacctggtc
     3541 cttcatcaat gattgaaggg gtgagatgac caccgtaaga ccttttgaat tcattccact
     3601 cattaaagcc ggcacttgga aagtaacgga cttccctcca ccggtgggaa aaacggcaag
     3661 aagggatttg ttggctacag ccgctttaac agccttctcc tgtaatggtt caccgtcata
     3721 ggttctgtag gaatcgtatc cgaaatactt tttcaatgct ttatgaatat caagggcttc
     3781 gttgcaataa tcgcagcctt ccaaacacgg gttgttcctg agcaaaaaca tgactctttc
     3841 aacctctgga aaggttttca aaacccaggg cggagtaatt gagtagtggc atcttgtatt
     3901 gataagagca aggcagtagg ataattcaat gggattttgc gatattattt ttccaagatc
     3961 ggccttttga catatctgaa atcggaactt attccggatt gttttttcca agtcccgaag
     4021 ctcaagctca tatccggcca aatgtacgaa agttttaggt acgaccgggt tattttccat
     4081 tccgcttttt gaagtcgcat caatatattt aaagaatgcg gaaaaaccac ctttatctct
     4141 taacagagta taaaaaattc gcttcaattc attatccaat tcataaaaag cgttaatctc
     4201 gtcaaagaaa agatcccggg ctttaattga atcattaagc ggattattta tatcctccgt
     4261 ttgcagctta tcgtctttta caagagaatg gtagggcttt ttgggaaaca aaagaggaga
     4321 aagaaataag gtatcgatag aattcgaatt attaatgcct gcagcttcaa cagcattttt
     4381 tatatatttt aaatcatgtc ttataatgtt atgcccgcaa acatactccg atccttgtaa
     4441 gaattcaata aaggcagcaa cagaaccgga gtgaaatgaa gcaccattgt ccttaatgct
     4501 tccaatatca agaatcttcc cgcttttagg atcgatttcc gtgtcaataa aagctattct
     4561 cttcacttcc agtcctcccc ggaatacaaa taataattag ctgatttata taatgcttac
     4621 attaagccct atggatttta ttctgttgaa atcagaaatt tccttctttt agacattttt
     4681 tgatttattc acatattgct ttttgaattt ataggtgata aaataaataa gatacgatgg
     4741 tgaatatgaa aacacaatcc ccttttacta tatcatattt ctatttgtat gtcaatatat
     4801 ttttagaaaa attttattta ttaaaatgcg gaggtgcctg ccattgtctt ttgaaattat
     4861 gaatacatcc tcatcactta tacaaaaaca aatactggaa gaaattaaaa agtgcaatga
     4921 ttataccgca cagtttggat taaccctgtc ccactcggat gctcttgaac tgattgaaac
     4981 atgcaatcat tcattaaaac gcaccggacg gatcgaattc ggcggtagta ttgtaagtaa
     5041 gataatcatg gagttttgcg actcccctta tatctccatg cacagctatg cccaaacgat
     5101 ccacgaactg actgaaatat tttatttata taaaaatgaa accctggatt tgataagcga
     5161 tgatgctcta atcaaattta tgaaaaaatg ctttgacggc aaatgccagg ggtccctcga
     5221 attgctttcg gagcgtgagc tttataatat ggcacataac ttaagatacg gactggcccc
     5281 ggattattcg gaggattcaa atacggatga ggaagatgag gaaaatgagt aatcttgaaa
     5341 agcaacacat gattaataca aataatacaa ataatttaag aaatgaagcc tattttgctt
     5401 cacttcttaa agaggcttat tcccaaggaa ttctgaatga tttagaagca gaaaatatac
     5461 aattgcaatg cataaacttt ttagcaaaaa agtgcgaaaa attcaacgac ggaatgagca
     5521 gttcgataag agtggaaatt gctgaaagaa ttatggaatc caatctttat acaattgggt
     5581 tgtacttaaa atcgttgccc gatgtgcagt gtgccataaa tgagcttaaa acgttattga
     5641 tttccgaaat gtacgaaaag ggaaagaaac tgattaatac caaatttgct tcaacaaatc
     5701 acctttataa gatggtccaa aagaacaaat tgaccacaat aaactacagc tataatgcaa
     5761 cacttagtga cagcggcata ggaagttttt ttaaattgta caatccggat tatggggctc
     5821 atgattgtgc ctcatccgtt gattatcagc tttgcaatcc cattaccgac cttgtgggag
     5881 ttgaatatat tcaaaaatat cttgaaagtc tgtatctgga aaataaattt tgcagaaatt
     5941 ttgctgcaga atacattcac aaacttcttt acgggtacta tgaaggatat aaggatttgc
     6001 tgattaatat ctttgaacat gtgttaacag cagcaatagg atgtgttctt gcaaaacgca
     6061 atattttagg cttggatatt tcgcaggaag aagtccaaag ccttcaaaat gaattatcaa
     6121 aggatgatgc tcctttgatt gctttaaaga ttcaaaacgc tgcggccgga gtactcgaag
     6181 atctgaatat ccaaagtcct tcacttcagg ggtatattga aaaaagtctt tcgaaaatta
     6241 caacaaatat catacatgcg gttaagacaa ataccctcgg taaaaccttt gtatctttgg
     6301 ttaatccgga cttaaaaccc aaaatacagt tttcattcgg taagaaaatg gatgatgaag
     6361 atttctggaa gttcatcgat gaattatccg aatgcagatt ttcatcggat aaaattgcat
     6421 ttatacaaca gaatgtaaaa tccttggggg atcttgaaga tatattgctt gatgtgcaac
     6481 ttggcaagga ggaaacttat ttggtcttag ataagctgac agatattgag cttgccgcat
     6541 tggttaaacg ccatacgttt aaatcggacc ttcaggcagt ggatttatca gaggcagagg
     6601 aaacattgca aaacaaccta aagaa
//
LOCUS       DAOOJC010000082         6592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_9647_length_6592_cov_3.574728, whole genome shotgun sequence.
ACCESSION   DAOOJC010000082 DAOOJC010000000
VERSION     DAOOJC010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9647_length_6592_cov_3.574728"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="PLA01_03515"
     CDS             complement(<1..1286)
                     /locus_tag="PLA01_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HPD00400.1"
                     /translation="MEIKRILAKITAIILIIGVFSVLPINTGIIYADFELVNDTFEVN
                     FDGWCNEGVDTVLEAVENQGNNATRGMMVANRSSEKDGAYSQKGFYLDGGTEYNYSVF
                     VKHDGTGIETFNLSLSYLDAETEERISKVIAAEDVAGGEWVRISTKYKAPKTALDITL
                     SITTDSTSNFIFDDVSITQKAKIGTNTVSAAGNRVLKDIYANYFRVGSVLNSGTVQNS
                     TIKALIMKEFNSITCENEMKPDATMVQSGSTNTNIKVSLNRAASILNFCAQNNMGVRG
                     HTLVWHAQTPQWFFKDNFSDYGSWVSQSVMDQRLESYIKNLFAEIQRQYPSLNLYAYD
                     VVNEAVSDDSNRTKNYGGAREPGYGNGKSPWVQIYGDNKFIEKAFTYARQYAPANCKL
                     FYNDYNEYWDHKRDCIASMCASLYNKGVLDGVGMQS"
     gene            complement(1680..5993)
                     /locus_tag="PLA01_03520"
     CDS             complement(1680..5993)
                     /locus_tag="PLA01_03520"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HPD00401.1"
                     /translation="MQKVTIQDKMLPELFPEIKDNDVVKKYFGSARIQGINLYKKSRK
                     LEIQITSENLIPAEALENLEQTLRGIFVLEDVYIKAFFNVDLSVEELVAKYWKSIVYT
                     VRKKIALCRGIMDGCVWSIEDKRLQIQLKTKGSEILKSKKCDGLIEKILEDGFGVKIR
                     VEFIDSMINEEVINEYIELRENEEAKVVTDTVINNIQKEARVAESTRSYTSNAKAADS
                     DIILGKSFNDSIMKMSEVTQDSGRVALCGDIIRTEERTLNSGKTLYIFDITDYSGSIT
                     VKIFLNKSKHENVIERLKENSSVKIRGEAQYDKFSKELTVIASDIIQVQKKTKKDNAE
                     EKRVELHLHTQMSAMDGVTPVKELVKRAHEWGHKAIAITDHGVVQAYPDAADAGKKYG
                     IKVIYGVEAYLLDDDVPIVYFANGHLVDGDFVVFDIETTGLQPDKDKITEIGAVKLRN
                     GKVVDTYSTFVNPQVPIPELIVKLTGITDEMVAEALTIESVLPEFLEFSKGAVLVAHN
                     ASFDTGFIRHNARLLGMDMENPVVDTLELSRKLFPELSRHKLNIVAKHLGVELKNHHR
                     ALDDAMACAGIFTKCIDILKAKNVRTIDDIDNAFGDCENSYKSHSTYHAIILVKNYIG
                     LKNLYKIISESHLKYFHKRPRLPKKLLMSYRDGLILGSACEAGELYRAILEKKSDEEI
                     SKIVRFYDYLEIQPLGNNQFMIDNGTVNSQEELKDINRKIVALGEKYNKPVVATCDVH
                     FIDPEDEVFRRILMAGQGFSDADNQAPLYLRTTEEMLEEFEYLGEKKCHEVVVENTNK
                     IADICEDIIPIPNGTFPPKIEGAEEEIRTLAEKRAKEIYGEKFPEVVEKRMEKELNSI
                     IKNGFSVMYIIAQKLVKKSLSDGYLVGSRGSVGSSFIAYLIGITEVNSLPPHYICESC
                     KYCEFIQDGSYGCGFDLPNKVCPNCGKELKRDGYDIPFETFLGFDGDKEPDIDLNFSG
                     EYQPVAHKYTEELFGVGHVYRAGTIGTIAEKTAFGFVKNYLDERELVKTNAEIGRLVK
                     GCTGVKRTTGQHPGGVMIVPQDREIYEFCPIQRPADDTTSNIITTHFDYHSISGRLLK
                     LDILGHDDPTVIRMLEDLTGVDARTIRIGEEKTMGLFSSTEPLNVRPEDINSEVGTFA
                     VPEFGTKFVRQMLVDTRPTTFSELIRISGLSHGTDVWLNNAQDLVRNKVATLSEVICT
                     RDDIMLYLIHCGLPHKTAFKIMEDVRKGKGLKEEYEQIMKENKVPNWYIESCKKIKYM
                     FPKAHAAAYVMMAFRIAWFKVYYPEAFYVTYFTVRADEFDANMMCHGQAKVRSCIREL
                     EQKGNNMTQKEKNVLTILEVANEMYARGISFLPIDIYKSDATKFQITPDGIRPPLNAL
                     QGLGITAAQNIVEARNQGEFLSVDDLRVRAKVSKTVIEILQQNGCLDGWSESNQISLF
                     G"
     gene            complement(6014..>6592)
                     /locus_tag="PLA01_03525"
     CDS             complement(6014..>6592)
                     /locus_tag="PLA01_03525"
                     /EC_number="1.17.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin/ferredoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPD00402.1"
                     /translation="HARILEELDFYDIVFSLKASSVPMTIAAYRLMSEKTDYPLHIGV
                     TEAGTLFRGTIKSCAGLGCLLAEGIGDTIRVSLTGDPREEVRVGNELLKSLGLLEGGV
                     EFVSCPTCGRCQIDLIKIASEVEERLGKLDKSIKVAVMGCAVNGPGEAREADIGIAGG
                     KNGALLFKKGEIIRKIPQDRIVDELMEEIMKM"
BASE COUNT         1731 a         1645 c         1106 g         2110 t
ORIGIN      
        1 tgggattgca ttcccacacc gtcaagtact cccttgttgt ataaagatgc acacattgaa
       61 gcaatacagt ccctcttgtg atcccagtac tcgttgtaat cattgtagaa gagtttacaa
      121 tttgccggag catattgtct tgcatatgta aatgctttct caataaactt attgtctccg
      181 tatatttgaa cccatggaga ttttccgttt ccatatcccg gctctcttgc accgccgtaa
      241 tttttggtcc tgttggaatc atcacttact gcctcattta caacatcata agcataaaga
      301 tttaatgacg gatactgtct ttgtatttca gcaaacaagt ttttaatata gctttccaaa
      361 cgctgatcca taaccgattg ggacacccag ctgccatagt ccgagaaatt atccttaaaa
      421 aaccactgag gtgtctgggc gtgccatact aatgtatgac ccctgacgcc catgttattt
      481 tgtgcacaga aatttaaaat gcttgctgca cggtttagag aaaccttgat attggtattg
      541 gttgacccgg attgaaccat tgtggcatca ggcttcattt cattttcaca ggtaatactg
      601 ttaaactctt tcataatcaa agcctttatt gttgaatttt gtactgttcc ggagttaagt
      661 actgatccca ctctgaaata gtttgcatat atatccttta atacacgatt acctgctgcc
      721 gataccgtgt ttgtcccgat ttttgctttt tgagttatag atacatcgtc aaaaatgaaa
      781 ttcgaagtac tgtcggtagt gatagataaa gtaatatcca atgcagtttt gggtgcttta
      841 tattttgttg aaatgcgaac ccattctccg ccggcaacat cctctgctgc aattactttt
      901 gaaattctct cttccgtttc tgcatccaga tagctcaaag aaaggttaaa ggtttcaata
      961 ccggttccgt cgtgttttac aaaaacgcta taattatatt ccgtaccgcc gtcaagataa
     1021 aaaccctttt gcgaataggc accgtccttt tcgctggagc ggtttgctac catcatacct
     1081 cttgtagcat tattcccttg gttttcaaca gcctctaaaa ccgtatcgac tccttcattg
     1141 caccatccgt caaagttcac ttcaaatgtg tcgttgacaa gctcgaaatc ggcgtatatt
     1201 atgcccgtgt ttataggcaa tactgaaaac acgccgatta tcaaaataat ggctgtaatt
     1261 ttcgccaaaa ttcttttgat ttccatacta ttcctcctct aaaaaataat taaataataa
     1321 aattaattct ttatttttgt attatttatt cgtaagatat ctatttttca gttgggaatt
     1381 ttcacgaatt ttagatttat tattgtgaaa aaagcacatt tttcggactt tagaaatatt
     1441 atctctctaa ttcaagacta taactgtgtc acttattgta ctaaaagaaa aactccccga
     1501 tataatccta tattattatt atacactttt tcgacatgta aaccaagagt catgtccaat
     1561 tttccatatt ccgtatttcg gtatacaata ctatagccgt gtacatatgt acctatccta
     1621 acaggcttat tctgtaaaca caacggactt ttaatccgtt ggccggacgt tttatatatc
     1681 taaccaaaca ggcttatctg gttgctctca gaccaaccgt caaggcatcc gttctgctgc
     1741 agaatctcta ttactgtctt gcttactttt gccctgaccc tcaggtcatc aacagacaaa
     1801 aactctccct ggttcctcgc ctcaactata ttctgagctg ccgtaatccc cagaccctga
     1861 agggcattca aaggaggtct gataccgtca ggagttatct gaaactttgt ggcatcggac
     1921 ttgtaaatgt caatgggtaa aaagcttatt cccctggcat acatttcatt tgccacctca
     1981 agaatggtaa gaacgttctt ttccttttgg gtcatgttat tgcctttttg ttctaactcc
     2041 cgaatgcagc ttcttacctt agcctgtcca tggcacatca tgttggcgtc aaactcgtcc
     2101 gccctgacag taaaataggt aacataaaac gcctcaggat aatacacctt aaaccacgca
     2161 attctaaaag ccatcataac ataggctgcc gcatgggcct tagggaacat gtattttatt
     2221 tttttacatg actcaatata ccagttgggt accttatttt ctttcattat ttgttcgtat
     2281 tcttccttaa gccctttgcc ctttctcaca tcctccataa ttttaaaggc tgttttatgg
     2341 ggaagcccgc aatgaatcaa atacagcata atatcgtccc tggtacatat aacctcagat
     2401 agcgtcgcca ctttattcct gacaagatcc tgggcattgt tgagccatac atcggttcca
     2461 tgggacagtc ccgatatcct tatgagttcg gaaaaggttg tcggtctcgt atccaccagc
     2521 atctgccgaa caaattttgt cccaaactcc ggtacggcaa aggttcccac ctcgctgttt
     2581 atgtcttccg gccggacatt taaaggctcg gtactgctga aaaggcccat tgttttttct
     2641 tctcctatgc gaattgtccg ggcgtcaacc ccggtaaggt cctcaagcat ccttatgacc
     2701 gtaggatcgt catgtcccaa tatgtccagc ttcaaaagcc ttccgcttat ggagtggtaa
     2761 tcaaaatggg tggtaataat gtttgacgtg gtatcgtctg caggacgctg tatcggacag
     2821 aactcgtata tttcccggtc ctggggaaca atcataacac ctccggggtg ctgacctgtg
     2881 gtcctcttta cgccggtaca ccccttaacc agcctgccta tctccgcatt ggtcttaacc
     2941 aactcccgtt catcaagata attcttgaca aaaccaaagg ccgtcttttc cgcaatggtt
     3001 ccaatggtac ctgcccggta tacatgtccg accccaaaca gctcttccgt gtatttgtgg
     3061 gcaaccggct ggtattcccc tgagaagtta aggtcaatat ccggctcttt atctccgtca
     3121 aatcccaaaa aagtttcaaa gggaatatca tatccgtccc tttttagctc cttaccgcaa
     3181 ttgggacaaa ccttgttcgg caagtcaaaa ccgcagccat agcttccgtc ctggataaat
     3241 tcacagtatt tgcagctttc gcagatatag tgaggcggca gggaattaac ctcggttata
     3301 ccgataagat atgctataaa ggaagaaccg acagatcccc tcgaacccac aagataaccg
     3361 tcgctcaggg atttttttac aagcttctgt gcaataatat acataactga aaaaccattc
     3421 tttataatgg agttcagctc cttttccatc cgtttttcca caacctcggg aaacttttct
     3481 ccatatatct cctttgccct tttttccgcc aaagtccgaa tctcctcctc ggcaccttct
     3541 atcttgggcg ggaaagtacc attgggaatg ggaattatat cctcacagat atccgctatc
     3601 ttgttcgtat tttcaacaac cacctcatgg catttctttt ccccgagata ttcaaattcc
     3661 tccaacatct cttctgtggt acgcaaataa aggggggcct gattgtccgc atcggaaaaa
     3721 ccctgtcctg ccataagtat tcttctgaag acctcatctt ccggatctat aaaatgaacg
     3781 tcgcaggttg caaccaccgg cttgttatac ttctctccca aagccacgat cttcctgttt
     3841 atatctttaa gctcctcctg gctgttaacc gtcccgttat ctatcatgaa ctgattattg
     3901 cccagaggtt gtatttcaag ataatcatag aaccttacga tttttgaaat ctcctcatcg
     3961 cttttctttt caagtattgc cctgtaaagc tctcctgcct cacacgcact gccaagaata
     4021 agcccatctc tgtatgacat aagaagcttt ttcggcagtc tcggcctttt atgaaaatac
     4081 tttagatgcg actctgaaat aattttataa aggttcttaa gacctatgta attcttaaca
     4141 agaataatgg cgtgatatgt ggaatgtgat ttataactgt tttcacaatc accaaaagca
     4201 ttatctatat catcaatagt cctaacattt tttgctttca aaatatcaat acatttggta
     4261 aaaatacctg cacaagccat agcatcatca agggccctgt gatggttttt caattcaacc
     4321 cctaaatgct tcgcaacaat attcagctta tgccttgaaa gctcgggaaa taacttccgg
     4381 gaaagctcca gggtatccac aacgggattt tccatgtcca ttcccagaag ccgtgcgttg
     4441 tgccttataa atcccgtatc aaaggatgca ttatgtgcca ccagcactgc cccttttgaa
     4501 aactccagaa actccggtaa aaccgattca atggttagtg cctctgccac catttcatcg
     4561 gtaatgcccg taagctttac aataagctcc ggtatcggta cctgaggatt tacaaaggtg
     4621 ctgtatgtat ccactacctt cccgttccta agcttaactg caccaatctc ggtaatcttg
     4681 tccttatcgg gttgaagacc tgtggtctca atatcaaaca cgacaaaatc accatcaacc
     4741 aaatgtccgt tagcaaagta gactatcggg acgtcgtcat ccaaaagata tgcttcaaca
     4801 ccgtaaataa cctttattcc atattttttc ccagcatcag ccgcatccgg gtatgcctgc
     4861 acaaccccat ggtcggttat ggcaattgcc ttgtgtcccc attcatgggc ccttttcacc
     4921 aattccttaa ccggagtgac accgtccatt gcactcatct gtgtgtggag atgcagttcc
     4981 accctttttt cttccgcatt atcttttttg gtcttttttt ggacctggat aatgtctgaa
     5041 gcaattaccg taagttcttt tgaaaattta tcatactgtg cttcgccccg gatcttcacg
     5101 gaagaattct ccttcaacct ctctataacg ttctcatgct tgctcttatt cagaaaaatc
     5161 tttacggtaa tcgaaccgga atagtccgtt atgtcaaata tgtaaagagt cttaccgctg
     5221 ttaagggttc tttcctccgt ccttataata tcgccgcaca gggcaactct gccggaatcc
     5281 tgtgtaacct cggacatttt cattatactg tcattaaaac ttttacctaa aataatatcc
     5341 gaatccgccg cctttgcatt tgaagtatag gacctggtag actctgccac ccttgcctcc
     5401 ttctgaatat tgtttatgac agtatcggtc acaactttgg cttcttcatt ttcccttaac
     5461 tcaatatact catttataac ttcctcattt atcatgctgt ctataaattc aacccggatc
     5521 tttactccaa aaccgtcctc cagtattttt tcaatcaggc cgtcgcattt cttgcttttt
     5581 aatatctcac tgccctttgt tttaagttgt atttggagcc ttttatcttc gatgctccat
     5641 acacatccgt ccattattcc cctgcataat gcgatttttt tccttactgt atatacaata
     5701 ctcttccagt attttgcaac cagctcttcc acggataaat caacattaaa aaaggcctta
     5761 atatagacat cctcaagtac aaatatccct cttaaggtct gttccaaatt ttccaaggcc
     5821 tctgccggta tcagattttc cgacgttatt tgaatttcca gcttcctaga ttttttataa
     5881 aggtttatgc cctgaatcct cgcagaaccg aaatatttct ttacaacatc attgtcctta
     5941 atctccggga aaagctccgg gagcatctta tcctgtattg taaccttttg catattaaac
     6001 ccctcttttt gcactacatt ttcataattt cctccataag ctcgtccact attctgtcct
     6061 gaggaatttt tcttattatt tcaccttttt tgaaaagaag tgccccattc ttacctcccg
     6121 caataccgat atcggcttct cttgcctcac cgggtccgtt tactgcacac cccataacag
     6181 caacctttat cgatttgtca agctttccaa gcctctcttc cacctctgaa gctattttta
     6241 tcagatctat ctggcatctt ccgcatgtcg gacaggaaac aaactcaact cccccctcta
     6301 aaagaccgag ggactttaac aattcgttcc ctactcttac ttcctctctt ggatcgccgg
     6361 taagggaaac ccttattgta tcccctatgc cttctgccag caagcatccc aaacctgcac
     6421 aggatttaat agtacctctg aacaacgtcc ctgcctcggt aacaccgata tgcagcggat
     6481 aatccgtttt ttcggacatc aacctgtatg ctgcaatggt cattggcaca ctggatgcct
     6541 ttaaggaaaa gacaatatcg taaaaatcca attcctccag tatcctggca tg
//
LOCUS       DAOOJC010000083         6542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_9732_length_6542_cov_3.279790, whole genome shotgun sequence.
ACCESSION   DAOOJC010000083 DAOOJC010000000
VERSION     DAOOJC010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9732_length_6542_cov_3.279790"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PLA01_03530"
     CDS             complement(<1..590)
                     /locus_tag="PLA01_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPD00403.1"
                     /translation="MSSNAVFKFWLENEYFDMETRNELNSIKDNPDEIEERFYKDLEF
                     GTGGLRGIIGAGTNRMNIYSVRKATQGLADYVNSRGMADSGVVIAHDSRRKSPEFALE
                     VAKVFTGNGIKVYLFDELRPTPELSFAVRHLKAAAGVMVTASHNPKEYNGYKVYGEDG
                     GQLPIEASNEVLSYIGRIEDITKIKTMEKDEAIEKG"
     regulatory      877..974
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1221..2147)
                     /locus_tag="PLA01_03535"
     CDS             complement(1221..2147)
                     /locus_tag="PLA01_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPD00404.1"
                     /translation="MNMVMYLLLVSVFISSCLIFYAFLGVYYRNRKVISIRLDKISKE
                     ENKSLESELNQPLFVRIVRPMLDDITKVVLKVTPKEIISVFERKVITAGNPFNLAIKD
                     WINIQIMIVIGLPLITAAFSMYMGFDARTVVFICIIELCFGVLFPNMLLNRKIKERQK
                     VICNSLPDVLDLLTVSVEAGLGFDGALAKVIDKMPGPIATEFEDVLNEMKVGKQKRDA
                     LKDMAQRVNIPDLTTFIGSIIQADQLGVSIGNVLRIQSEQMREKRRQRAKEKAMKAPV
                     KMLIPMVLFIFPTIFSVLIGPVIIKVIYTFTK"
     gene            complement(2152..3129)
                     /locus_tag="PLA01_03540"
     CDS             complement(2152..3129)
                     /locus_tag="PLA01_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPD00405.1"
                     /translation="MLFIISILTFVTAMILCYPILGYLIGKQSSIVRLKRYISIEENR
                     EAKKKTKQKEYKAGLSIIARGVKEARFLDGYKKKVQLELQRAHILLKPEEFITICIIF
                     MLVLSTFSFALFSSSSMVVIIALIGGLTGWFIPVIYLKLKIRKRIKHLNDQLNDAIVL
                     ISNSLKAGYSFFQAVDIVSKEMTGPMAEEFALLQKEVNFGTSTENALENLVNRVSSDD
                     LELVVTAVMIQRQVGGNLAEVLDNISNTIRERISIKGEVRTVTAQGRMSGLIISLLPV
                     GLVLILFLINKQHISILFTDPLGLAILGFSVFMEFMGIYFISKIVKIEV"
     gene            complement(3180..4538)
                     /locus_tag="PLA01_03545"
     CDS             complement(3180..4538)
                     /locus_tag="PLA01_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HPD00406.1"
                     /translation="MSLLERLQKEKNTETLDNKSQRVKKIQHVQEDPYASIKSKIHSR
                     IIDEIKSDALKDLDDDGSEALEHEITSIVENILNEENSYITKNERQKIISEIIDETIG
                     LGPINPLIHDPTISEIMVNGPNKVYVKKKGRLVISDVRFKDDQHVMHVIEKIVAPIGR
                     RIDESSPMVDARLPNGSRVNVIIPPLALDGPSITIRKFSEKPYDVKDLINFGTMTPNV
                     AMFLKACVEARLNIVVSGGTGSGKTTTLNVISSFIPEDERIVTIEDAAEIQLAQEHVV
                     RLETRPPNIEGKGAVTIRDLVRNSLRMRPDRIVVGEVRSGEALDMLQAMNTGHDGSLT
                     TGHANTPRDMLARLETMVLMAGMDLPVKAIREQIASAIDIIVQQSRFKDGSRKITHIS
                     EVTGMEGDIITMQDIFIYKQSGKDERGRIIGDIVPTGIKPKFMEKIERAGIMLPSDLF
                     TP"
     gene            complement(4556..5761)
                     /locus_tag="PLA01_03550"
     CDS             complement(4556..5761)
                     /locus_tag="PLA01_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD00407.1"
                     /translation="MEKIKVVIVDDTEETRNNVKTLLSFEKRIEVIGEAENGEEAVFI
                     VRESQPDVVLMDINMPVMDGIKATEEISMNAPETAVVIMSVQGETEYLRKAMSAGARD
                     FLNKPFSGDDLVNTIMRAYDIESQRKQRSKVTMVKEEVKSKVISVFSTKGGVGKTTIA
                     SNLAVSLARASKKRVALVDLDLQFGDVAIMLNASIKNTISDLIKEINMLDSDIMEDYL
                     VTHFSGVRILPAPVKPEYAEYVTSSHVEKIINTLKEYYHYIIVDTSASFHETVLAALD
                     ISDRILLVSTLDLPTIKNVKAGLDVMETLKYPKEKINIVVNKASEQFGIKYKDFENTL
                     KHQIWGFIPEDSQTVITSANKGFPFVMTRAETKVAKAILTITNEILKEKEPESKDNEK
                     NAIKKIFGM"
     gene            complement(5807..>6542)
                     /gene="cpaB"
                     /locus_tag="PLA01_03555"
     CDS             complement(5807..>6542)
                     /gene="cpaB"
                     /locus_tag="PLA01_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HPD00408.1"
                     /translation="ESVNKRVIVISLVLAIFTTGLVYLYIQKATVKAEVTEYINVYVA
                     AKTLPAKHLITEADIKVSRVTREYLSPQAVLNEADIIGKRLKDRVIEGEQILRDRLVE
                     DKDLTLAFNLPVGKRAIAINVSEQIAVGYLIKPGDTVDVIANFSGEDNEPNMTKIVIQ
                     NVTVLAIGKNMGSAEENSQEIPNTVTLAVSPEDAEKLSFVSEFETLRLILRNIEDEKI
                     VNTDGVIKKDMTGTKGMENALPAVEP"
BASE COUNT         1781 a         1557 c         1060 g         2144 t
ORIGIN      
        1 aatcccttct ctattgcctc atccttctcc atggtcttaa tctttgttat gtcttcgatc
       61 ctgcctatat atgataagac ttcgtttgat gcctcaatag gcagctgccc cccgtcttca
      121 ccatacacct tgtaaccgtt atattccttt ggattatggc ttgccgtaac cattacacct
      181 gcagcagcct ttaaatgcct aaccgcaaag gaaagctccg gtgtaggtct cagctcatca
      241 aaaaggtaca ccttaattcc attgccggta aagacctttg caacctccag ggcaaactcc
      301 ggtgatttac gccgcgaatc atgggcaata acaactccgc tgtcggccat gccacgactg
      361 ttaacataat cggccaaacc ctgtgtagcc tttctgaccg agtaaatgtt cattctattg
      421 gtacctgcac ctataatgcc tctaagacca ccggtaccaa attccagatc cttatagaat
      481 ctctcttcta tttcatccgg attgtcttta atgctattga gctcattcct ggtctccata
      541 tcgaaatatt cattttccaa ccaaaattta aaaactgcat tactggacat aaacttctct
      601 cctcaatcaa acaaaattca attaaaaaaa atctcattcc ttagtatacc acattctatg
      661 catattacaa caattttatt atgtagttta tttgatttac ttcaaaaaat tatgtatcga
      721 tctcaggcat agtctaacta tgcagggaat caaagtttta atcatttaca gtgtaaatcg
      781 acaaatataa tacttggcaa tttgcataaa taatgctata ataaaattgt aatacattct
      841 acctccctaa ttaaggtata aggagataaa taggcttatg aaacagggca aaattgccga
      901 aaggcaatga cgcaaagcca tggatctaag tcggaaaagc aaagcttttt cgatatgatt
      961 gccaggttac cattaaaagt tctgtttata ctcttaataa tatatttgga aataggtctt
     1021 tatttcctta taccactttt tttaagatag atacaaaaaa taagggcaca agcccttttt
     1081 tcatttccca aaaaaattat acccgtaaaa cacagctaat tactttgcga atgagaaacg
     1141 aagaaaaata tgtaagaaac aatttccgta atatttttct tcatcttcat tctttattta
     1201 cacctataaa actcaaatca ttactttgtg aaggtataaa taaccttaat tataacagga
     1261 ccaatcagaa cggaaaaaat ggtcggaaaa ataaacaaca ccataggtat tagcatcttg
     1321 accggtgcct tcatagcttt ctcctttgcc ctctgtctcc tcttctccct catctgttcg
     1381 gattgaattc taagtacatt accaatgctt acaccaagct ggtccgcctg gattattgag
     1441 cctataaagg tagtaaggtc aggaatattc accctctgtg ccatatcctt aagggcgtct
     1501 cttttttgct ttccaacctt catctcgttc agtacgtctt caaattccgt tgcaataggt
     1561 ccgggcattt tatcgataac cttggcgagg gccccatcga atcccagtcc cgcttctacg
     1621 ctaaccgtaa gtaagtccag aacatccggg agagaattgc aaatcacttt ttgcctttct
     1681 ttgatttttc tgtttaacag catgttggga aaaagcaccc cgaagcacag ttcaattatg
     1741 catataaaga caacggttct ggcatcaaaa cccatgtaca tgctgaatgc agctgtaatc
     1801 agaggcaaac cgataacaat cattatttga atattaatcc aatcctttat tgccaggtta
     1861 aatgggttac ctgcggtaat aacctttctt tcaaacaccg aaattatttc ttttggcgtc
     1921 acctttaaaa ccactttggt tatatcatcc agcatcggcc ttacaatcct gacaaacaag
     1981 ggctggttca attcactttc caatgatttg ttttcttcct tgctgatttt gtcaagcctt
     2041 atgcttatta cctttctgtt tctataataa actcccagga atgcatagaa aataaggcag
     2101 gacgaaataa ataccgaaac caaaagcaaa tacattacca tattcatctg ctcacacctc
     2161 gattttcaca attttgctta tgaaatatat ccccatgaac tccataaata cagaaaaccc
     2221 aagtattgca agccccagag gatctgtaaa caatatgctt atatgctgct tgtttatcaa
     2281 gaacagaatt aatacaagac ctacaggcaa tagggaaatt atcagcccgg acattcttcc
     2341 ctgggcagta acggtcctta cttctccttt gatgcttatt ctctctctta tagtattgga
     2401 tatgttgtcc aagacctcgg ccagattacc acccacctgt ctttgaatca ttactgcagt
     2461 gacaaccaac tcgaggtcat cgctcgaaac cctgttaacc agattttcca aggcattttc
     2521 cgtacttgtt ccgaaattca cctctttttg aagcagtgca aattcctccg ccataggacc
     2581 ggtcatttcc ttgctcacaa tatccaccgc ttgaaaaaag ctgtatcccg ccttcaaaga
     2641 gtttgatatc aaaacgatgg catcattgag ctgatcgttc aaatgcttaa ttctttttct
     2701 tattttcagc ttaaggtaaa taacgggaat aaaccatcct gtcagtcctc caatcaatgc
     2761 aataataacc accatggagg acgaactgaa aagtgcaaag gaaaaggtac tcaaaaccag
     2821 cataaaaata atgcatatgg ttataaactc ctcaggcttc aataatatgt gtgccctctg
     2881 aagctccaac tgtaccttct ttttgtatcc atccaggaat cttgcttctt taacccctct
     2941 tgcaattatg ctaagtccgg ctttgtactc cttttgctta gtctttttct tggcttcccg
     3001 gttctcctca atgctgatat acctttttaa ccgcacaatg gagctctgct taccgattaa
     3061 atagcctaat atcggataac atagtatcat tgcagttaca aaagttagta tagaaataat
     3121 aaacagcatt tcatagccct ccattaaagg ttttgttgta tacctacagc ataaatggat
     3181 tacggtgtaa aaagatctga gggaagcatt attcctgccc tttcaatctt ttccatgaac
     3241 ttcggtttaa tccctgtggg cacaatgtct ccgattattc tgcccctttc atcctttccg
     3301 ctttgtttat aaatgaagat atcctgcatg gttataatat cgccctccat tcctgtgacc
     3361 tcggaaatgt gggtaatctt tctgcttccg tctttaaacc tggattgctg tacaataata
     3421 tcaattgccg atgctatttg ttcccttatt gcctttacag gaagatccat acctgccata
     3481 agcaccatgg tctcaagtcg ggcaagcatg tccctcggtg tgttggcatg gccggtggta
     3541 agagaaccgt catgaccggt attcatcgcc tgaagcatat caagggcctc accgcttctg
     3601 acctctccga caactatcct gtcaggcctc attctaaggg agttcctgac cagatccctt
     3661 atggtaacgg caccctttcc ttcgatattc ggaggtctgg tctcaagcct tacaacatgc
     3721 tcctgagcaa gctgaatttc cgcagcatcc tctatcgtga ctattctttc atcttcaggg
     3781 ataaaggagg aaataacatt cagggttgtg gttttaccgc ttccggttcc cccggagact
     3841 actatgttca gccgtgcttc aacacatgcc tttagaaaca tggcaacatt cggtgtcatg
     3901 gtaccaaagt ttataaggtc ttttacatca taaggcttct ctgagaactt tctaatggta
     3961 atgctgggcc cgtccaatgc aaggggcggg attattacgt taacacggga gccgttggga
     4021 agccttgcat ccaccatcgg cgagctttca tcgatccttc ttcctatggg tgcgactatc
     4081 ttttctataa catgcataac atgctggtcg tctttaaatc tcacatcgga aattacaagc
     4141 cttccttttt ttttcacgta aaccttattc ggaccattta ccattatttc agatattgta
     4201 ggatcatgga tcagtggatt tatcggacca aggcctatgg tttcgtcaat tatttccgaa
     4261 attattttct gtctctcgtt ttttgttatg taggagtttt cttcgtttaa tatgttttca
     4321 acaatggatg ttatctcatg ctctaaagcc tcgctcccat catcatcaag atcctttaag
     4381 gcatcactct ttatctcatc aatgatcctg ctgtgtattt tggacttgat gcttgcataa
     4441 ggatcttcct gtacatgctg tatttttttt accctctggc ttttgttatc caatgtttca
     4501 gtgttttttt ctttttggag cctttcaagc aatgacatat ttattccccc attgattaca
     4561 tgccaaatat tttcttaatg gcattctttt cgttatcctt actttccggt tccttttcct
     4621 tcagtatttc atttgtaatg gttaatattg cctttgccac ctttgtttcc gctctggtca
     4681 taacaaaggg aaatccctta tttgccgacg tgataacggt ctgactgtcc tcaggtatga
     4741 atccccatat ctgatgcttc aaagtattct caaaatcctt atacttgatt ccaaactgtt
     4801 ctgaggcttt atttacaact atatttattt tttccttcgg atacttcaat gtttccataa
     4861 catcaaggcc ggcttttaca ttcttaattg tcggaagatc aagggttgaa accagaagaa
     4921 tcctgtcgga aatatcaagt gctgccaata ccgtttcatg aaagcttgcg gaagtatcta
     4981 caatgatata gtgatagtat tctttcaatg tatttattat cttctccaca tgggatgatg
     5041 tcacgtactc tgcatactcg ggtttcacag gtgccggcag gatacgtacc ccggaaaaat
     5101 gggttaccag gtaatcttcc attatatcac tgtccagcat gtttatttcc ttaatcaggt
     5161 cacttatggt gttttttatt gatgcattca gcattattgc cacatcaccg aattggagat
     5221 ccagatccac aagagccacc cgtttcttcg atgccctggc caggctgact gccaaatttg
     5281 aagcaatagt tgtttttccc acaccgcctt tggtactgaa aaccgaaatg accttcgatt
     5341 taacttcttc ttttaccatg gtgacctttg atctctgctt tctctgtgac tctatatcat
     5401 aagctctcat aatggtattg accaaatcat cgccggaaaa aggcttgttc agaaaatccc
     5461 tcgcaccggc tgacatcgcc tttctcaaat actctgtttc tccctgaaca gacattataa
     5521 ccactgccgt ttccggagca ttcatggata tttcctcagt ggcctttata ccatccataa
     5581 ccggcatgtt tatgtccatc aacaccacat ccggttgtga ttctctgaca ataaaaacag
     5641 cctcctcgcc gttttcagcc tcacctatca cttcaattct cttttcaaat gacagcaacg
     5701 tcttgacgtt atttctggtt tcctcagtat catcaacaat aacaacctta attttttcca
     5761 tacgatctcc tccatcttta cgtggtgtta agttcctgtc aatacactac ggttctaccg
     5821 ccggtaatgc gttttccatt cctttagtgc cggtcatgtc ctttttaatc acaccatcgg
     5881 tattgactat tttttcgtcc tctatattcc tgagaattag cctgagggtt tcaaactccg
     5941 atacaaacga aagcttttct gcatcctccg gactaacagc cagtgttacc gtgttcggaa
     6001 tttcctgaga attctcttct gccgatccca tatttttacc tatggcaaga accgtaacat
     6061 tttgaattac tattttggtc atattcggtt cattatcttc tccgctaaaa ttcgctataa
     6121 catccactgt gtccccgggc tttatcaaat accccacagc aatttgctca cttacattta
     6181 tcgctattgc cctttttccc acaggaagat taaaggccaa agtcaaatct ttatcttcaa
     6241 caagtctgtc ccgcaaaatt tgctcacctt cgattaccct gtccttcagt ctttttccaa
     6301 taatatctgc ctcatttaaa accgcctgag gacttaaata ttctctggtt acccttgaca
     6361 cttttatatc ggcttccgtt atcaaatgct ttgcgggtaa tgttttagct gcaacataaa
     6421 cattaatata ctccgttacc tcagccttta ctgtagcttt ttgaatatat aagtacacca
     6481 aaccggttgt aaatattgct aaaacaagtg agattacaat tactcgttta ttaaccgact
     6541 cc
//
LOCUS       DAOOJC010000084         6489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_9840_length_6489_cov_3.807429, whole genome shotgun sequence.
ACCESSION   DAOOJC010000084 DAOOJC010000000
VERSION     DAOOJC010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9840_length_6489_cov_3.807429"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PLA01_03560"
     CDS             complement(<1..225)
                     /locus_tag="PLA01_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD00409.1"
                     /translation="MKNTIIEADNVSYKYGTDDENRQSSPAVKDLDIKINDGEFVAVI
                     GRNGSGKSTFARLLNALLIPTSGVLYITNRR"
     gene            complement(391..915)
                     /locus_tag="PLA01_03565"
     CDS             complement(391..915)
                     /locus_tag="PLA01_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HPD00410.1"
                     /translation="MPVQRKIGRTTDQRKAMLKGLVTSLFQNGKIETTEAKAKEVKNI
                     AEKLIAIAVKECDNFTSKQVKISAAKLDSKGQKITNVKKSKNGKEYQVVEREIKTDMV
                     RVDNPSRLHARRKVMNWVYKIKDENGKSISITNKLFDEIAPKYKDVNGGYTRIYKLGP
                     RRGDAAEMVILQLV"
     gene            complement(968..1915)
                     /locus_tag="PLA01_03570"
     CDS             complement(968..1915)
                     /locus_tag="PLA01_03570"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPD00411.1"
                     /translation="MIEIEKPKIECIISSEDNTYGKFVVEPLERGYGITLGNSLRRIL
                     LSSLPGVAVTSIKIDGVLHEFSTIPGVVEDVTEIILNIKELSLNLHSEGTKVVYIDAD
                     GEGEIKASDIKVDSDVEILNPDHKIATLNGESRLYMELTLEKGRGYVSAERNKHAGQP
                     IGVIPIDSIFTPVRKVNYTVENTRVGQVTDYDKLTLEVWTNGSIKPDEAISLGAKIMS
                     EHLNLFIDLSDNAKNAEIMVEKEENKKEKVLEMTIEELDLSVRSYNCLKRAGINTVED
                     LTNRTEADMMKVRNLGRKSLEEVLNKLKALGLSLAPSEE"
     gene            complement(2069..2695)
                     /gene="rpsD"
                     /locus_tag="PLA01_03575"
     CDS             complement(2069..2695)
                     /gene="rpsD"
                     /locus_tag="PLA01_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPD00412.1"
                     /translation="MARYTDASCRLCRREGEKLYLKGERCTTNKCSISRRAYAPGQHG
                     QNRKKLSEYGIQLREKQKARRYYGILESQFRKYFDMAVKKKGITGENLLQILELRLDN
                     VVYRMGLASSRPEARQLVRHGHFSVNGKKVNIPSYLVKLGDVVAVTEKSKSSPKMKDN
                     KELAAGKPSPKWLEYDVEEMTGKIAALPLREDIDLPIKESLIVELYSK"
     gene            complement(2775..3179)
                     /gene="rpsK"
                     /locus_tag="PLA01_03580"
     CDS             complement(2775..3179)
                     /gene="rpsK"
                     /locus_tag="PLA01_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPD00413.1"
                     /translation="MASKTAGGKRTTRKRRERKNIERGAAHIRSTFNNTIVTLTDVAG
                     NALAWSSAGSLGFRGSKKSTPFAAQMAAETAAKGAMEHGLKIVEVYVKGPGSGREAAI
                     RALQAAGLEVSLIKDVTPIPHNGCRPPKRRRV"
     gene            complement(3201..3572)
                     /gene="rpsM"
                     /locus_tag="PLA01_03585"
     CDS             complement(3201..3572)
                     /gene="rpsM"
                     /locus_tag="PLA01_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPD00414.1"
                     /translation="MARIAGIDLPREKRVEIGLTYIYGIGRTLSNQILAKTGVNPGTR
                     VRDLTDDEINKLREVIDKEYKVEGDLRREVSLNIKRLMEIGCYRGRRHRMGLPVRGQR
                     TKTNARTRKGPKRTVGVQRKK"
     gene            complement(3750..3863)
                     /gene="rpmJ"
                     /locus_tag="PLA01_03590"
     CDS             complement(3750..3863)
                     /gene="rpmJ"
                     /locus_tag="PLA01_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPD00415.1"
                     /translation="MKVKPSVKVICEKCKIIRRKGRIMVICQNPKHKQRQG"
     gene            complement(3887..4105)
                     /gene="infA"
                     /locus_tag="PLA01_03595"
     CDS             complement(3887..4105)
                     /gene="infA"
                     /locus_tag="PLA01_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPD00416.1"
                     /translation="MSKEDVIEVEGKVVEALPNAMFEVELDNGHKILAHISGKLRMNF
                     IRILPGDKVTVELSPYDLTRGRITWRAK"
     gene            complement(4149..4457)
                     /locus_tag="PLA01_03600"
     CDS             complement(4149..4457)
                     /locus_tag="PLA01_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HPD00417.1"
                     /translation="MLVMAEVSIVDIGIGQVVYSIAGRDAGRKLIVVEIINDRMVKVS
                     DGDLRRIENAKIKKVKHLKITNSVIISLAEKIQKGARVSNAEIRKALADIDNEEESRL
                     "
     gene            complement(4462..5232)
                     /gene="map"
                     /locus_tag="PLA01_03605"
     CDS             complement(4462..5232)
                     /gene="map"
                     /locus_tag="PLA01_03605"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPD00418.1"
                     /translation="MKIIKTKKEIELMKKAGEVVALTLNKIEEAVKPGVTTLELDRIA
                     EEFIRKCGAIPSFKGYKAGFNSVPDFPGSICSSVNSEVVHGIPGLRELKDGDIISIDV
                     GAFLDGFHGDAARTFAVGIISDEAQRLIDVTKQSFFEGIKNAVNGNRIIDISGAIEDY
                     VVSNGFSVVREFVGHGIGREMHEEPQIPNYRCRGQRSRIETGMTLAIEPMVNEGTHLI
                     KILDNGWTVVTLDGKLSAHYENTIVVTEDEPLILTRLN"
     gene            complement(5234..5878)
                     /locus_tag="PLA01_03610"
     CDS             complement(5234..5878)
                     /locus_tag="PLA01_03610"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPD00419.1"
                     /translation="MRMILLGAPGAGKGTQAVILSERFNIPHISTGDIFRSNIKNNTE
                     LGKKAKEYIDKGLLVPDELTIDIVRDRINKSDCSDGFILDGFPRTIQQAQALDIILKD
                     MGIELDCVLNINVSDDKIIKRLSGRRVCQCGKTYHVKNNPPKQEGVCDSCNGKLFQRD
                     DDREETVIQRLETYHQQTEPLIGYYKNCGKLITVEGQDSIDDTTKEVLDALNGV"
     gene            complement(5954..>6489)
                     /locus_tag="PLA01_03615"
     CDS             complement(5954..>6489)
                     /locus_tag="PLA01_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HPD00420.1"
                     /translation="MYGGQSTHIPIKVNSAGVMPIIFATSFVALPATIVGFFFPTSTH
                     PVAEYFRDFQSRIEISLLSGLLIMFFTFFYTFIQFNPIELANNLKKNGGFVPGIRPGK
                     PTSDYIYKVLTRITWFSALFLAIIQIFPSLLQTITGVRGVWFAGTSVLILVGVALETV
                     RQIESQMLMRHYKGFLE"
BASE COUNT         1712 a         1558 c          952 g         2267 t
ORIGIN      
        1 tcttctgttt gtaatataca aaaccccact ggtcgggata agcagggcat ttaatagcct
       61 tgcaaaggtt gattttccgg atccgtttct gccgataaca gccacaaact caccatcgtt
      121 aatctttatg tctaaatcct tcaccgccgg agagctttgt ctgttttcat catccgttcc
      181 atatttatag cttacattat ctgcctctat aatcgtgttt ttcataaatt ctcccctatt
      241 gacaatactg ttcatgctga tttcgctatc atagcaaaac agggattaag gcgaaccatg
      301 tcatcaccca atccctatta actacatctt cttccgcttc cctcattaaa agaaaagttt
      361 gctttcctct taaccttaag gttacagata ttaaaccaat tgcagaatca ccatctcagc
      421 tgcatcgcct cttctgggtc caagcttgta tatccttgtg tatccgccgt taacatcctt
      481 atatttcggt gcgatttcgt caaataattt gttggtaatg ctgattgatt taccgttctc
      541 atccttaatt ttgtataccc aattcattac ctttcttctg gcatgaagcc ttgacggatt
      601 atcaactctc accatatcgg ttttaatttc tctttccacc acttgatact ctttgccgtt
      661 tttggacttc ttgacgtttg taatcttctg tcccttcgaa tccagttttg ccgcactgat
      721 tttaacctgt tttgaagtga agttgtcaca ttccttaaca gcaatagcta taagcttttc
      781 tgcaatattt ttaacttctt tggctttagc ctcagtcgtc tcaatttttc cattttgaaa
      841 aagtgacgta acaagacctt tgagcattgc ttttctctga tctgtagtac gccctatttt
      901 tctttgcact ggcattctta tacctccttt actctaagcc cgctatcaac tccggactta
      961 tttttggtta ttcttcactc ggtgctaatg acaaccccag agccttcagt ttgttaagaa
     1021 cctcttccaa agactttctg ccaaggtttc taaccttcat catatctgct tcagttctgt
     1081 ttgttaaatc ctcaacggta tttattccag ccctctttaa gcagttatat gacctaacag
     1141 acagatctag ctcctcaata gtcatttcca gcactttttc cttcttgttt tcttcctttt
     1201 ctaccattat ttcagcgttt ttagcattat cagaaaggtc aataaacaag ttaagatgtt
     1261 cgctcattat tttagcaccc aggctgatag cttcatcagg tttaatactt ccattagtcc
     1321 aaacctctag ggtcagcttg tcataatctg ttacctgacc gacacgtgta ttctcaacag
     1381 tgtagttaac cttcctaaca ggcgtgaaaa tcgaatcaat aggaatcact cctattggtt
     1441 gaccggcatg cttgtttctt tccgctgata cataccctct tcctttttcg agggttaact
     1501 ccatataaag tctgctttcg ccgttcaagg ttgctatttt atgatcgggg tttagaattt
     1561 cgacatcaga atcgacttta atatccgatg cctttatttc tccctcaccg tcagcatcaa
     1621 tatacacaac cttagttcct tcactgtgaa gatttaaaga caactcttta atattgagaa
     1681 ttatttctgt aacatcctca actaccccgg gtattgtcga aaattcatgc agcacaccgt
     1741 ctattttgat tgaggttaca gctacaccgg gcaaagatga aagtaatatt ctccttagtg
     1801 aattaccaag agtgatacca tatccccttt ccaacggttc tactacgaac ttcccataag
     1861 tgttgtcttc actagatatt atacattcta tcttcggctt ttcaatttca atcatcaaaa
     1921 accctccttt tttactttaa taatttttga gggcaactga ttcaaaattc cttatctctc
     1981 aattatgcta taacttgaac cttcatctct atcgtttgaa tcctcagttg ctgtaccagg
     2041 aatatttaca tacacttaag ccctgttatt acttggagta caactcaact attagacttt
     2101 ctttgatcgg cagatcaatg tcttccctta atggaagagc agcaatttta cctgtcattt
     2161 cttcaacgtc atactcaagc cacttcggac tcggctttcc tgctgccaat tctttattgt
     2221 ccttcatttt cggtgaactt ttactcttct ctgttactgc tacgacgtct ccaagcttca
     2281 ccagatagga aggaatatta acctttttgc cgttaaccga aaaatgtccg tgtctaacca
     2341 actgtctggc ttccggtctt gatgatgcaa gacccattct atatacaaca ttatctagtc
     2401 tcaactctag aatctgaaga aggttttcac cggtgatgcc tttcttttta acagccatat
     2461 caaagtattt tctgaattgg ctctccaaaa taccatagta ccttcttgcc ttttgctttt
     2521 cacgaagctg tataccatat tcggacaact tctttctgtt ttgaccgtgt tgtccgggag
     2581 catatgctct ccttgaaata gagcatttat ttgtagtgca tctttcccct ttgaggtaaa
     2641 gtttttcacc ttccctgcgg cagagtctgc aagatgcatc ggtatatctt gccatctagt
     2701 tttacacctc caaaagaata tttatttata atataaacaa aaatactaaa taatagattc
     2761 agtaacctgt tgcactatac ccttcttctc ttaggcggtc tgcatccgtt atgaggaata
     2821 ggcgtaacat ctttaatgag acttacttca agtccagctg cttgcaatgc tcttatagct
     2881 gcttcccttc cggatcctgg accctttaca taaacttcga caatcttaag tccatgctcc
     2941 attgcacctt tagcagcagt ttctgccgcc atttgtgcag caaagggtgt gctcttcttt
     3001 gaacctctga agcccaagct tcctgcacta gaccatgcca aagcattacc tgcaacatct
     3061 gtcaatgtta ctattgtatt gttaaatgtt gagcggatat gtgcagcacc acgttcaata
     3121 tttttacgtt ctcttctttt cctggtagtt ctcttgccac cagctgtttt actagccatt
     3181 taatcgcaaa cctcctttta ctattttttc ctctggactc caacagttct cttcggacct
     3241 tttcttgttc ttgcattggt ctttgtcctt tgaccacgca ccggaagtcc cattctatgt
     3301 cttcttcctc tatagcaacc tatttccatt aatcttttga tattgagaga tacttctctt
     3361 ctcaaatctc cttcaacctt atattccttg tcaattactt ctctaagctt attaatctca
     3421 tcgtcggtca ggtctcttac cctggtgcca gggtttacac ctgtctttgc caaaatttga
     3481 tttgacaaag ttctgccgat accatagatg taggtcagcc caatttctac ccttttttcc
     3541 ctgggtaaat caattccggc aatacgtgcc atcttttttt acacctccaa taaatttctg
     3601 tgagtttttt caattttcga tatttagctt atataaaata aacaatccag gttataagaa
     3661 tatggaaata aaatgttcat attcttatac agccgcaccc tgtatgttat tttttcgctc
     3721 aaaaaacaaa acttaataca atataaaact tagccctgtc tttgcttatg tttcggattt
     3781 tggcagataa ccataattct accttttctt cttataatct tgcatttttc acaaatcacc
     3841 tttacggacg gttttacttt catatcgaca cctcctcaaa actaaattac ttagccctcc
     3901 atgtaatcct accacgggtc agatcataag gagaaagctc aactgtcacc ttatcaccgg
     3961 gtaaaatcct aataaaattc attctgagtt taccggatat atgagccagt attttatgtc
     4021 cattatccaa ttccacttcg aacattgcat ttggcaatgc ttcaacaacc ttaccttcaa
     4081 cttctataac atcttcctta gacaaactat taaaccctcc ttatctctga ttttaagcta
     4141 aacactatct ataatctact ttcttcttcg ttatcgatat ccgccaaagc cttacgtatt
     4201 tctgcatttg atactcttgc acctttttgt attttctcag ctaaggatat aataacacta
     4261 ttggtgattt taaggtgttt tacctttttt atcttcgcat tctcaatcct tctgagatcg
     4321 ccatccgaaa ccttaaccat gcggtcatta attatttcaa ctacaataag ctttctgcct
     4381 gcatcccttc cagctataga atatacaacc tggccaatac caatgtccac aatactcacc
     4441 tctgccatta caagcataaa cctaattcag cctggttaat atcaacggtt catcttcggt
     4501 taccacaatt gtattttcat agtgggcaga tagctttccg tctaatgtaa caaccgtcca
     4561 accgttatca agtatcttaa ttaaatgcgt tccttcatta accatcggtt ctattgcaag
     4621 agtcataccc gtttctattc tgcttctctg tccccggcat ctgtagttcg gtatctgcgg
     4681 ttcttcatgc atttcccttc caataccatg tcctacaaac tcacggacaa cagaaaaacc
     4741 attactcact acataatctt ctatagcacc tgaaatatca attattctat ttccgttaac
     4801 agcattcttt attccttcaa aaaagctttg ctttgtgaca tctatcagcc tttgagcttc
     4861 atcggaaatt atacctacag caaaggttct tgcagcatct ccgtgaaacc cgtccagaaa
     4921 agcacctacg tcaatactaa taatatcgcc atcttttagt tcccttaaac ccggtatacc
     4981 gtgaacaact tcactgttaa ctgaggaaca tatacttccc ggaaagtcag gaacactgtt
     5041 aaatcccgct ttatagcctt taaatgaagg aatggcacca catttgcgta tgaattcttc
     5101 tgcaattctg tccagttcca atgtggtaac ccccggcttt accgcttctt caattttatt
     5161 caaagttaaa gccacgactt caccggcttt tttcattaat tcaatttctt tctttgtttt
     5221 tataatcttc atatcagact ccatttaatg catccaaaac ctcttttgtt gtatcgtcaa
     5281 tgctgtcctg cccttcaacc gttatgagct tcccgcaatt tttataatat cctattaatg
     5341 gctccgtctg ttgatgataa gtttcaagcc tttggatgac ggtctcttca cggtcatcat
     5401 ccctttggaa caatttcccg ttacagctgt cgcagactcc ttcttgttta ggaggattat
     5461 tctttacgtg gtaagttttc ccgcactgac aaactcttct cccggacaat cttttgataa
     5521 tcttgtcgtc tgaaacattt atattcagga cgcagtccaa ttctataccc atatctttta
     5581 aaataatatc aagtgcttgt gcctgttgta tagttcttgg aaaaccgtcc agaataaaac
     5641 cgtcggaaca gtcggatttg ttaattctgt ccctaacaat atctattgta agctcatccg
     5701 gaaccaataa tcctttatca atatattcct tagctttctt gccaagttcc gtgttgttct
     5761 taatgttgct tctgaatata tctccggttg atatgtgagg tatattaaat ctttcggata
     5821 aaattactgc ctgtgttcct ttacccgcac ctggggctcc tagcagaatc attctcatat
     5881 taatcctccc tcaaatactt caattaaatt acatccaact attgcatcaa ttaattcgta
     5941 cttacaccta aatttactcc aaaaatcctt tataatgcct catgagcatt tgtgattcta
     6001 tctgtctgac tgtttccaac gcaacaccaa caagaatcaa aacactggtt ccggcaaacc
     6061 aaacccctct tactccagtt atagtctgta ataaagatgg gaatatttga atgatcgcaa
     6121 ggaacaacgc cgaaaaccat gttattcttg tcaatacctt gtagatgtaa tcggatgttg
     6181 gttttccagg acgtattccc ggtacgaaac caccgttttt cttcaggttg tttgccagtt
     6241 caatcgggtt gaactgaata aatgtataga aaaatgtaaa gaacataatt aacaaacctg
     6301 ataaaaggga aatttcaatc cttgactgaa agtccctaaa gtattctgcc acagggtgag
     6361 tagaagttgg aaagaaaaat cccactattg ttgccggtaa agctacaaag gatgtagcaa
     6421 agattatagg cataacccct gcggaattaa ccttgatggg tatgtgagta ctttgaccgc
     6481 catacattt
//
LOCUS       DAOOJC010000085         6453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_9907_length_6453_cov_3.428415, whole genome shotgun sequence.
ACCESSION   DAOOJC010000085 DAOOJC010000000
VERSION     DAOOJC010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9907_length_6453_cov_3.428415"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLA01_03620"
     CDS             complement(<1..661)
                     /locus_tag="PLA01_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPD00421.1"
                     /translation="MLKRCSKLDGTVSKHIFNNINTRWKVNLSFIIIIITSISALGYV
                     SFNMVLNAMVNHAREESEMHISQIATNIKVVLNGLDDIAKDVLDNKEFKECVRNYENL
                     TDFHMRAESERIIEGVLKNRIRTRADIADIAAVTNKGGYITSSEVKQRAGENGLSYYA
                     IKMFKYSNKDSMWLDTYQSEVISTTTYTDNLLVISNIRSIKDAGNKDIGILLLNVRES
                     YL"
     gene            complement(686..2512)
                     /locus_tag="PLA01_03625"
     CDS             complement(686..2512)
                     /locus_tag="PLA01_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPD00422.1"
                     /translation="MFIRRKQVVSDMINGLEQEKNSTAWKLNIGFALIVVLCIFVVGL
                     ASFRMISESMLNHAKASSTELIKQTCRNIETILKGFDEIAMTLSQDNTIAETISEFDS
                     ITDVHEKAEKERKICEILNNRIKTRNDITDIAVISNQGDYISSGIMGPSVNRDIKSSY
                     AVKKFIESKRGSLWLDTYTTDIDSTFTYSKSGQVFSLMKGILTPSSLKSKGILLVNFS
                     ESYLYNIIADIKLSDNGKMFIVGSNGNYVLNPEDRTKNGTKAEYEFIDEILGKGSNCE
                     IKVIGNVEHLVTFQTIEEIKGTDLGWTVVEITPVASITTSVTNAGMRLFFIGLICVAV
                     GFILIGFITKLYNTFLDKKYSERHSIAMERERLASLGQLIGGIAHNFKTPIMSISGGL
                     EAIMDLINEYESSIGDSEVTAQDHHEIAAEMRDWVEKIKPYCSYMSDIISTVKGHTVN
                     MNDSSNVSFTVEELLKRVNILMNHELKKFHCKMNVDLKVDINTVIPGEINNLVQVLNN
                     LISNSIEAYKGGDGSIDLSISRKGQEIEIVVKDYGEGIPDKIKKKLLKEMITTKGKNG
                     TGLGLYMSYSTIKGKFGGTMKIESEEGKGTAIYIYIPYLMKK"
     gene            complement(2681..3895)
                     /locus_tag="PLA01_03630"
     CDS             complement(2681..3895)
                     /locus_tag="PLA01_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD00423.1"
                     /translation="MKLKHVWIVFKKEVRDIIRDKRTIITSIFVPMILIPVMNMLVGG
                     NVERLNRDITENITIALSESSNTEEIKNIVKNEIIKDYPNISLIEVDNPTQAISESKV
                     RVVLDFEVDYSAKLNEGKPFVIKLIYDKSQTKSEGSLGFIWEAIDNFNRKTVEKRLNS
                     YGISKDVLTPVVIEESNIASEEESAGSLLALIFPLLIVLLMSSGGIAPATDLVAGEKE
                     RNTLEPLLTTKPGRSSLLVGKYLTVTLFSFITVITTMVGVILGYIVNPSSFSMGSSTS
                     ITGVSIPPLALFLVIIIAMALGMTFSALQIALSTYAKSFKEAQTYLSFLMIGVMIPGY
                     ATMFMQPKEIPVYMFFVPVLNALSALKTVFGYSINYTYLFITLGSSVVYVIISLWLTS
                     SLFKKEKYLFRS"
     gene            complement(3885..4616)
                     /locus_tag="PLA01_03635"
     CDS             complement(3885..4616)
                     /locus_tag="PLA01_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD00424.1"
                     /translation="MIDIQGLSKHFDKVVAVDGISFKVNKGEIFGLLGENGAGKTTTL
                     RMLATMLTPTSGTAKMAGFDIINEPEKVRSSIGILFGGESGLYDRLTAEENISYFGEL
                     NDMDRTRIKERIKELARIFNMEDYLKRRAGKLSKGMRQKVAFARAIIHDPDIMLFDEP
                     TSGLDVSAIRDVHEFIKSCKAEGKTVIFSSHSMDEVDKLCDRVGVIHKGKLVADGTLD
                     GIKSEYGDQDLENIFIRLVGGKSEA"
     gene            complement(4653..5360)
                     /gene="ispD"
                     /locus_tag="PLA01_03640"
     CDS             complement(4653..5360)
                     /gene="ispD"
                     /locus_tag="PLA01_03640"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPD00425.1"
                     /translation="MRIGREFFVSAVVAAAGKGTRMNMDINKQYIEVCGIPVLARTLL
                     VFEKCELIDDVVVVVNNNDIFYVKQNIVEAYDLKKVKLLVAGGAQRQNSVYNGLLQLN
                     KETDVVVIHDGARPFINNKIIAECIEAALEHGVSTVAVPVKDTIKSSEDGDYVNETLE
                     RSKLWAVQTPQAFKYDILMSAHHRAAQDGFTGTDDTVLAERLGYKTKLVMGSYNNIKI
                     TTKEDLKFVEAIVNTLD"
     gene            complement(5662..6144)
                     /locus_tag="PLA01_03645"
     CDS             complement(5662..6144)
                     /locus_tag="PLA01_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPD00426.1"
                     /translation="MYNIGDKIVYPMHGAGVIESIEEKEILGNKQSYYVVKIPIGEMK
                     VLIPTKNVDGIGIREVISEEDADKVFSMLKNKTVSVSSNWNKRYRENMVKIKSGNIYD
                     VAEVVRCLMLRDKEKGLSTGERKMLNNAKQILISELVLVKNMKPVEIESIINKFLSNK
                     "
BASE COUNT         1851 a         1459 c          961 g         2182 t
ORIGIN      
        1 aaagatagga ttcccttaca ttaagcaaaa ggataccaat atccttgtta cctgcatcct
       61 ttatgctcct gatgttcgat atcaccagga ggttgtccgt ataggttgtg gtggatatta
      121 cctccgactg gtaagtgtcc agccacattg aatccttatt gctatattta aacattttta
      181 ttgcataata tgacagaccg ttttcaccag ctctctgttt tacttcactg gaagtaatat
      241 accctccttt gttggtaact gcggcaatat ctgcaatatc cgcccttgtc cttatcctgt
      301 tttttaatac cccttctatt attctttcgc tttcagctct catatgaaaa tccgttaaat
      361 tttcatagtt tcttacacat tctttaaatt ccttgttatc cagcacatcc tttgcaatat
      421 catccagacc gtttaacacc accttaatat ttgtggcaat ttggcttata tgcatttcag
      481 attcttcccg ggcatggttc accatggcat ttaataccat attaaaagat acatacccca
      541 gtgccgatat tgatgtaata ataatgatga tgaaagatag gtttaccttc cagcgtgtat
      601 taatattgtt gaaaatgtgt tttgaaaccg taccgtccag tttgctacat cttttcagca
      661 taaacgcagt tcccccaccc gtttgctact ttttcatcaa ataaggaata tatatatata
      721 ttgccgttcc ttttccttcc tcactctcaa ttttcatagt tccgccaaac ttacctttga
      781 ttgtggagta tgacatatat aggcctaatc ctgttccatt cttcccctta gtagttatca
      841 tttccttaag cagcttcttt tttattttat ccggtattcc ttcgccatag tccttcacca
      901 cgatttctat ttcctggcct tttctgctta ttgacaggtc aatactgcca tctccccctt
      961 tatatgcttc tatagaatta gaaataaggt tattcaatac ttgaacaaga ttattgattt
     1021 cccccgggat aacagtgttt atatcgactt tcaagtccac attcatttta cagtggaatt
     1081 tcttaagctc atgattcata aggatgttta ccctttttaa cagctcttct actgtaaagc
     1141 ttacattgga agaatcgttc atgttgaccg tatgtccttt aacggtagaa atgatatcgg
     1201 acatgtagga acaatatggc tttatttttt caacccagtc cctcatctct gctgcaattt
     1261 catggtggtc ttgtgcagta acctcagaat ccccaattga gctctcatat tcatttataa
     1321 gatccataat ggcctccaag ccgccggata tcgacataat tggcgttttg aaattgtgag
     1381 ctattcctcc tatgagttgt cccaatgagg caagcctttc cctctccatg gctatggaat
     1441 gcctttccga gtatttttta tccagaaatg tgttatagag ctttgttata aagcctatca
     1501 gaataaaccc caccgcaaca catatcaacc ctataaaaaa caacctcatt ccggcatttg
     1561 taacactggt ggttatggat gcaaccgggg ttatttcgac aaccgtccag cctaaatctg
     1621 ttccttttat ctcttcaatg gtttgaaaag ttacaaggtg ctccacattt ccgatcactt
     1681 taatctcaca gttgctgccc tttcccaata tttcatcgat aaactcatat tccgcttttg
     1741 tgccgttttt ggttctgtct tcggggttta atacataatt gccgttacta cccactataa
     1801 acatttttcc attatctgaa agttttatgt ctgcaattat gttgtaaaga taggactcgc
     1861 taaaattgac aagcaatatt cccttgcttt taagacttga cggcgttaat attcctttca
     1921 ttagggaaaa cacctgtccg cttttggaat atgtaaatgt agaatcaata tctgtggtgt
     1981 atgtatccaa ccacaatgat ccccttttgc tttcaatgaa ttttttaact gcatagcttg
     2041 atttaatatc ccggttaaca ctcggcccca ttatccccga tgaaatataa tccccttgat
     2101 ttgatattac cgcaatatca gttatgtcgt ttcttgtttt aatacggtta tttaaaatct
     2161 cacatatttt tcgttccttt tctgcttttt cgtggacatc agtgatactg tcaaactcgc
     2221 ttatagtctc tgctattgtg ttatcctggg ataatgtcat ggcaatttca tcaaagccct
     2281 tcagaatagt ctcaatattc ctgcaggttt gcttgataag ttctgtggaa cttgccttgg
     2341 catggttcag catcgattcg gaaatcattc gaaaggaagc cagacccaca acaaatatac
     2401 acaaaaccac tataagtgca aaaccaatat tcagcttcca ggccgtgctg tttttttcct
     2461 gttctaaacc attaatcatg tcggatacta cctgcttacg tctaataaac atagacaaat
     2521 cctccacctt aagcctgaaa tttatattga tgtataataa aaaaactact ctgaactgtt
     2581 gaatcagatt gtgaaaatac tgtgccaata tattaaagaa gtactttgag atgaatctat
     2641 tctattttta ttctacataa agtataaaaa ttcctcctta tcagctccta aaaagatact
     2701 tctctttctt gaaaagggat gaggtcagcc ataagctgat tattacataa acaacggaag
     2761 aacccaaggt tataaaaaga taagtatagt tgatactgta gccaaacact gtcttcaaag
     2821 ccgaaagtgc attcaataca ggaacaaaaa acatatatac gggtatttct ttaggctgca
     2881 taaacatggt tgcatagccc ggaatcatta cccctatcat caaaaaggac aaatatgtct
     2941 gtgcttcttt aaaagacttt gcataagtgc tcaaagctat ttgcagtgcc gaaaatgtca
     3001 ttccgagagc catggcaata attattacca gaaacagggc caagggcggt attgaaactc
     3061 ctgttatact ggtactgctt cccattgaaa acgaggatgg gtttacaata tatccgagta
     3121 ttacgcctac catcgttgtt attacagtga taaatgaaaa cagggttacc gtcaaatact
     3181 tgcccacaag aagagatgag cgaccgggct ttgtggtaag caacggttct aaagtatttc
     3241 tctccttttc tcccgccact agatctgtgg caggtgcaat tccgcctgaa gacattaaca
     3301 gcacaataag taacggaaat attaacgcaa gcaagcttcc ggccgattct tcctcactgg
     3361 caatattgct ttcctcaatc acaacagggg taagcacatc cttgcttatt ccatacgagt
     3421 tcaacctttt ctcaacagtt tttctattaa aattatcaat agcctcccat ataaatccaa
     3481 gactgccttc ggacttggtt tgggatttat catatatcag ctttattaca aagggttttc
     3541 cctcatttaa cttggctgaa taatccactt caaaatccag tactactctg accttcgact
     3601 cactaatggc ttgtgtcgga ttatcgactt caatcaggct gatgttggga taatccttta
     3661 ttatctcatt tttgactata ttcttaattt cctcggtatt ggaagactcc gacaatgcta
     3721 tagtaatgtt ctcagttata tccctgttca gtctttcaac atttcctcct acgagcatat
     3781 tcattacagg tatcaaaatc attggcacga atatacttgt aattattgtc cttttatccc
     3841 gaattatatc tcttacttcc ttcttaaaaa caatccagac atgtttaagc ttcacttttt
     3901 ccacccacca atcttataaa tatgttctca aggtcttgat ctccgtactc ggacttaatt
     3961 ccatcaagag taccatctgc cacaagcttg cctttatgaa ttacaccgac gcggtcacac
     4021 agcttatcaa cctcgtccat gctatggctt gaaaatatca ctgtcttgcc ttcggcttta
     4081 caacttttaa taaattcatg tacatccctt attgcactga catccagacc ggatgtcggc
     4141 tcatcaaaga gcattatatc cgggtcgtga ataatggccc tggcaaaggc aaccttctgc
     4201 ctcattccct ttgaaagctt tcccgcccgt cttttaagat agtcctccat gttgaaaatc
     4261 cgggcaagct cctttattct ttctttaatt cgtgttctgt ccatatcatt cagctctccg
     4321 aaataagaga tattctcctc tgctgtcaac ctgtcataca gcccgctctc tcctccgaaa
     4381 agaataccta tgctgctcct taccttttcc ggttcattta taatatcaaa accagccatt
     4441 tttgcagtgc cggaagttgg agtcaacatg gtcgcaagca tcctgagggt tgtggttttt
     4501 cccgcaccgt tctcacctaa aaggccaaat atctctccct tatttacctt aaaactaatg
     4561 ccgtcaactg ccacaacttt atcaaaatgt ttcgataaac cctgaatatc aatcatataa
     4621 accacctttc ttttaagtaa gttattatcg tttcagtcca acgtgttcac tattgcctcg
     4681 acaaatttca aatcttcttt tgttgttatt tttatgttgt tgtagcttcc catcacaagt
     4741 ttggttttat aacccagtct ctctgcgaga actgtatcat cggttccggt aaatccatcc
     4801 tgtgccgccc tgtgatgggc actcattaaa atgtcatatt taaaagcctg tggagtctgc
     4861 acagcccata atttgctcct ttcaagggtt tcattaacat agtctccatc ctccgaggat
     4921 tttattgtat ccttaacagg tactgccacc gtggaaacac cgtgctcaag agctgcttct
     4981 atgcattctg caattatctt attattgata aacggtcttg ctccgtcgtg tataacaaca
     5041 acatccgttt ctttgtttaa ttgcagcaag ccgttataca cggaattctg cctctgtgct
     5101 cctcccgcaa ccaggagctt tacctttttc aagtcataag cctcaactat attctgtttg
     5161 acatagaata tgtcattatt gtttacaacc acaacaacat cgtctattag ctcacacttt
     5221 tcaaatacca gaagggttct ggcaagtaca ggtattccac acacctcaat atattgcttg
     5281 ttgatgtcca tattcatcct tgtccctttt ccggcagcgg caaccactgc actcacaaaa
     5341 aactctcttc cgattctcat agttttctcc cgaaataata ttatcatttc ttagaattat
     5401 accatatctt tacttaaaat agcacaaata aataggatag caaaggagta actaaataaa
     5461 tgtgaaaaaa aaattccaga cactaaagtt acgcaatttt ctctccatat ttttttcaca
     5521 taaataatat aagccaagaa caaaggtaaa gaaaaataac ttgcacctac cggcagtgaa
     5581 tacataataa acagtgaaat ttctaaaaaa gaaaaaaaga ctgttataaa acagccttta
     5641 tattgagtaa aagaatctca attatttatt agataaaaat ttgttaataa tactttcgat
     5701 ctccactggt ttcatatttt taacaagcac taattcgcta attaaaatct gttttgcatt
     5761 gttgagcatt ttccgttcac cggttgagag tcctttttcc ttatctctca acatcaaaca
     5821 ccgtactacc tctgccacat catatatatt accgctctta atcttaacca tgttctctct
     5881 atatctcttg ttccaattag atgatacact gacagttttg ttcttcagca tggaaaacac
     5941 cttgtctgca tcctcttcac taataacctc tcttatcccg ataccgtcca cattctttgt
     6001 cggaatcaat accttcattt ctccaattgg aattttaact acatagtaac tttgtttgtt
     6061 acctaatatt tccttttcct cgatagactc tataactccc gcaccatgca tgggatacac
     6121 aattttatca ccaatattat acatatacac tcctccacta aagcaaaaga acaaactcgt
     6181 ctaaaatatt ctatctttgt actaataaaa aaacggattc tctgtttaag ccgaaagtat
     6241 aatgccgtat atcatttatc tctaaacata aaaatgatac taaagaatct tgaaaaacca
     6301 ttgtcatagc atatctctac atctctagta tactacaaat gagtataatt gtcaaaatat
     6361 tttattttag catgagatta agatagtgtc aactgaaatt tttgtcttgt ttgtgtcaag
     6421 taaaagttgg caatgatctc ttcatcctgt tat
//
LOCUS       DAOOJC010000086         6446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_9923_length_6446_cov_3.732123, whole genome shotgun sequence.
ACCESSION   DAOOJC010000086 DAOOJC010000000
VERSION     DAOOJC010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9923_length_6446_cov_3.732123"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /gene="ugpC"
                     /locus_tag="PLA01_03650"
     CDS             <1..1005
                     /gene="ugpC"
                     /locus_tag="PLA01_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HPD00427.1"
                     /translation="GPSGCGKTTTLRMVAGLEEISEGELFIGDKLVNDVAPKDRDIAM
                     VFQNYALYPHMSVFDNMAFGLKLRKMPKDEIKRRVLEAAKILDIEHLLERRPKALSGG
                     QRQRVALGRAIVRDPKVFLMDEPLSNLDAKLRVQMRTEISKLHQRLQTTFIYVTHDQT
                     EALTMGTRIVVMKDGYIQQVDTPTNLYERPINMFVAGFIGSPQMNFVNARVERRGEEI
                     HLLFGKEDIKLPEGKAKKLDGSDYIGREVVIGIRPEDIHDEAVYLESMSNSIVNAKVE
                     VVEMLGSETLLYTVVGEVDFTARVNPRTKARAGDVVKLALDTNKIHIFDKETEKTIMN
                     "
     gene            1541..2626
                     /locus_tag="PLA01_03655"
     CDS             1541..2626
                     /locus_tag="PLA01_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPD00428.1"
                     /translation="MSIKLYQNLINQIKDTIESEIGIMDENGLILLCSNEEKVGQVNP
                     LIDEIMKTDDTFSVIGGQSFQKIYIKNKLEYIVFINSDDSNNSKYLRLISINIVNIKA
                     YYDEKYDKINFIKSVILDNILPGDITLRAKELHITSNVYRVAFLIKTEPTKDIYAHEI
                     IEGLFPRKAKDFVVILDDETTVLIKELNSNYDYKEVNKNAKIILDNLSTEGMIKSKIG
                     IGTVVDNIKDIGRSFKEAQTAILIGEIFESEKNIIDYNKLGIGRLIYQLPPTLCRLFL
                     NEVFKDGAFEALDTETMNTIVKFFENNLNVSETSRQLFVHRNTLVYRLDKIQKITGLD
                     LRLFDDAIIFKVAILVKKYLDSNRELV"
     gene            2751..3458
                     /gene="ftsE"
                     /locus_tag="PLA01_03660"
     CDS             2751..3458
                     /gene="ftsE"
                     /locus_tag="PLA01_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HPD00429.1"
                     /translation="MVEFKNVVKKYSNGTLALSNVNFCINKGEFAFLVGPSGSGKSTI
                     LKLILKEEDTTEGEVFVNGYDLSMLKRKEIPSFRRSLGVVFQDFRLLPNKTVYENVEF
                     AMQITEALPKEIRRQVPMSLALVGLSRKAKSYPNQLSGGEQQRVALARALVNNPSLII
                     ADEPTGNLDPETSWEIMKLIQEVNMRGTTVIVATHEKSIVDKMKKRVIAIEKGEIKRD
                     QEKGQYENEVKDSKICC"
     gene            3427..4311
                     /gene="ftsX"
                     /locus_tag="PLA01_03665"
     CDS             3427..4311
                     /gene="ftsX"
                     /locus_tag="PLA01_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPD00430.1"
                     /translation="MKLRTAKYVVKEGFVNTYRNVLMSLASMGVVTASLVILGFFWII
                     TANINHNTKFLKDQPEMQVFCNPEMDGHQVGMLEWTIGRDKRIKEYKVVDKKQAFEEA
                     KELLGDDKDVLEGLDESIMPVSFIIKLNNSSDYEEVVDKYNNYPGVDSVRYSQKAIDF
                     INKILRGLQIGSSLLITILVIIAVFIISNTIKLTVFARRKEISIMKYIGATDWFIRLP
                     FIVEGVIIGLIGAVASFIVLFIIYNIGGGWVENDFLMVKLVGMNDVGFQLILLTCVLG
                     ALVGASGSVLSIRKYLRV"
     gene            4364..5491
                     /locus_tag="PLA01_03670"
     CDS             4364..5491
                     /locus_tag="PLA01_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPD00431.1"
                     /translation="MKKVLLVVMVLTLMFSAVMIPAFGDTISDAQNQKKTVDSKINDI
                     SKQKKEQKNKLSSIKSEKDYLESEERKKTQEYNSLMQQIDELNKEIETVDEAIKESEE
                     KYNYQMELFKVRLKVMYQNSNVTYIESLSESESIVDFWNRLEIVSAISKKDKELIEDI
                     KAAKTDVEFKKQLAVEKKTAVKESADASLNKLNEIKATRSGLDREISDISSQLKKLEQ
                     QEDELLRKSNELANQIKSLQRSGSYAGGSMLWPTPSCSRISSYFGNRLHPILKVYKMH
                     NGIDISAGNGASIIAANKGVVIVAGWQSGYGNTVIVDHGGGISTLYAHCSKLLVKVGD
                     SVKAGDTIAKVGSTGLSTGPHLHFEVRKNGTPVNPLDYVKP"
     gene            5720..>6446
                     /locus_tag="PLA01_03675"
     CDS             5720..>6446
                     /locus_tag="PLA01_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPD00432.1"
                     /translation="MNKNVITGKVLPILIAALLSSTITAYGFLVWHYKNPNYVVMSEK
                     EAEKYKDSKGFDPQYAITFDKDNVDSVIINKFNEVKKLLDTYYYKNINQNQLLEGAIA
                     GMTDSLNDPYTVYFTKEQMKEFNEKTQGSYVGIGVSISMDNDGILTVIEAFEDSPAKE
                     VGILPGDKIVKVDDKDVTTIRDDNLIVSMIKGEENTKVKITVFRPSEGTYLDFDVIRK
                     EIKVVNINSEVLEGNIGYIRIIMF"
BASE COUNT         2193 a          957 c         1485 g         1811 t
ORIGIN      
        1 ggaccgtctg gatgtggaaa gactactaca ctgagaatgg ttgcaggatt ggaagaaatc
       61 agcgagggag aattgttcat aggtgacaaa cttgttaacg atgtagctcc gaaggacaga
      121 gacatagcaa tggttttcca aaactacgca ctgtatccgc atatgtctgt ttttgacaat
      181 atggcatttg gtttgaaact cagaaaaatg cccaaggatg agataaaaag aagagttttg
      241 gaggcagcaa aaatacttga tattgagcat ttgctggaaa gaaggccgaa ggcattgtcc
      301 ggcggacaga ggcagagggt tgctttaggt cgtgctattg ttcgtgatcc taaggtattc
      361 ttgatggatg agcctctttc aaaccttgat gcaaagctca gggttcagat gagaaccgag
      421 atcagtaagc tgcaccagag acttcagaca accttcatct acgttactca tgaccagacc
      481 gaagcgttga ccatgggtac aagaatcgta gtaatgaagg atggatatat tcaacaggtt
      541 gataccccga ctaaccttta tgaaagacct ataaacatgt tcgtagcagg atttatcgga
      601 agcccgcaga tgaactttgt aaatgcaaga gttgaaagac gcggggaaga gatccatttg
      661 ctgttcggca aggaagacat aaagcttccg gaaggaaagg caaagaagct cgatggaagc
      721 gattatattg gaagagaagt ggtaataggc attcgaccag aagatatcca tgacgaagct
      781 gtttatcttg aatcaatgtc aaacagcata gtaaatgcaa aggttgaagt tgtagaaatg
      841 cttggttcgg aaacattgtt gtataccgta gtaggagaag ttgattttac agcaagggtt
      901 aatccgagaa ccaaggcaag agctggtgat gttgttaagc ttgctcttga taccaataag
      961 attcatatct ttgacaaaga aactgaaaag actataatga attaaatatt tttaacggat
     1021 atggcaaagg ggaaagaata atcgttcttt cccctttgtt agggtgcatt aaatgagtgt
     1081 gtagaaaaat ataccagaaa cttaagttac gcaagtctag agcctgtaaa tactccgtgg
     1141 ggtcgagcaa aactcacttc gttcagacaa tgctctccct ttccacgtcg tataaacagg
     1201 ctctacgact tgcttcacaa tgtttctttc atatttttct tcacttaaat aatatacacc
     1261 ccctttgcaa tatacccgga acggatgcat attcttattt attgggattt cacgaagtga
     1321 aatgagcttt tgtttattgg gatttcacga agtgaaatga ccaaaaagcg aattggctcg
     1381 atgccgatga gcttttgttt gatgtttttt gttttttgtg tataatgaaa aaggagataa
     1441 ttataaaggg ttcgtttaca gtattgattg aatacccctt gaatcaatta cgaagaatac
     1501 ctaagtacaa tttattaatc gtgtgacggg ggtgatggag ttgtcaatta agctttatca
     1561 aaatctcata aaccagatta aggacaccat tgaatcggaa atcggtatta tggatgaaaa
     1621 cggtcttatt ttgttatgct caaatgagga gaaagtcggg caggtcaatc cgttgatcga
     1681 tgaaataatg aaaaccgatg atactttctc ggtaattggc ggacagtctt ttcaaaaaat
     1741 ttatataaaa aataagttgg aatatattgt atttataaat tcggatgatt caaataacag
     1801 taaatacctc aggcttatat ccatcaatat tgttaacatt aaggcgtatt atgacgaaaa
     1861 gtatgataag atcaatttta ttaagagtgt catacttgac aatatcttac ccggagatat
     1921 tacattaagg gccaaagaac tgcatattac aagcaatgta tacagggtgg catttttgat
     1981 aaagacagaa ccaacaaagg atatttatgc acacgagata attgaaggcc tttttccaag
     2041 aaaggctaaa gactttgttg taatattgga cgatgagaca actgttttaa taaaagagct
     2101 taattccaac tatgattaca aagaagtaaa taaaaatgca aagataatat tggataattt
     2161 atcaactgag ggaatgataa agtctaaaat tgggattgga actgttgttg acaatataaa
     2221 ggacatcggg cgttctttca aggaagcaca gacggctata ttaattgggg aaatatttga
     2281 aagcgaaaag aatataatag attacaacaa gcttggtatt ggcaggttga tttatcagct
     2341 tccgcctacg ctttgcagat tgtttttaaa tgaagtgttt aaagatggtg cctttgaagc
     2401 tttagacact gaaaccatga acacaatagt taaatttttt gaaaacaacc tgaatgtaag
     2461 tgaaacatcc cgacagcttt ttgttcacag aaatactctg gtgtacaggt tggataagat
     2521 tcagaagata acaggccttg atctgagact gtttgacgat gcaataatat ttaaggttgc
     2581 aatacttgta aaaaaatacc tcgacagcaa ccgggagctt gtatgacagt ttggggcaat
     2641 ttccgtatat ataattgcca aatgataata tttgcgataa aatattgcac ggaagataaa
     2701 attaaagtac cggattaaat ccgaaggtat tgcttttagg agatgttaat gtggtagagt
     2761 ttaagaatgt ggttaagaag tattcaaatg gaacattagc attaagcaat gttaactttt
     2821 gtataaacaa gggcgagttt gcttttttgg tgggaccgag cggttcgggc aaatctacga
     2881 ttttaaagct tatattaaaa gaggaagata ctaccgaagg tgaggtcttt gtaaacggat
     2941 atgacctttc catgctgaaa agaaaagaga ttcccagctt cagaaggagt ttgggagtcg
     3001 tatttcagga tttcaggctg cttcccaata aaactgttta cgagaatgta gaatttgcca
     3061 tgcagattac agaagcactt cccaaagaaa tcagaagaca ggtcccaatg tccttggcat
     3121 tggtgggact gagcaggaaa gcaaaatcct atcctaatca actctccgga ggagaacagc
     3181 agagagttgc tcttgcccgt gcactggtaa acaatccttc acttattatt gctgacgaac
     3241 caacaggaaa tcttgaccct gaaacatcat gggaaataat gaaactgatt caggaagtca
     3301 atatgagggg tacaactgtg attgttgcca cgcatgagaa gagcattgtt gataaaatga
     3361 aaaaacgtgt aattgctatc gaaaaaggag aaattaaaag agatcaggag aaaggacagt
     3421 acgaaaatga agttaaggac agcaaaatat gttgttaaag aggggttcgt caatacctat
     3481 agaaatgtgc tgatgtctct tgcttcaatg ggtgttgtta cagcatcctt ggtaatcctg
     3541 ggttttttct ggattatcac ggctaatata aatcataata ccaagttttt gaaagaccag
     3601 cctgaaatgc aagtgttttg caatccggaa atggatggac atcaggttgg aatgcttgaa
     3661 tggactatag gtcgtgacaa aaggataaag gaatataagg ttgtagataa aaagcaggca
     3721 tttgaagagg ccaaggaatt gctcggggac gacaaagatg ttttagaggg ccttgacgag
     3781 agtatcatgc cggtgtcttt cattattaaa ctaaacaact cgtcagatta tgaagaggtt
     3841 gtggataaat ataataacta tcccggcgtg gacagtgtca ggtattcaca aaaggctata
     3901 gattttatta ataaaatact tagaggcttg cagataggaa gctcactttt gataacaata
     3961 cttgtgataa tagctgtatt cataatttcc aacaccatta agttaacggt gtttgcacgg
     4021 cggaaagaaa tcagcataat gaagtacatc ggtgctaccg actggtttat acgattgccc
     4081 tttatcgttg aaggagtaat tattggatta ataggggcag ttgcaagttt tatagtattg
     4141 tttattattt ataatatagg aggtggatgg gtagagaatg attttctgat ggtaaaactt
     4201 gttggaatga acgatgtagg tttccaattg attttattga cctgcgtttt aggagctctg
     4261 gtgggtgcgt caggcagtgt tttatcaata aggaaatact taagagtata aaaaaacagt
     4321 aaactaagga taaaattctg gttaaggcaa gggggttaag tcagtgaaaa aggtattatt
     4381 ggtagttatg gtattaacct tgatgttttc tgcagtaatg attcctgctt tcggagatac
     4441 catttccgat gctcagaatc agaaaaaaac cgttgatagc aagataaatg acatatcgaa
     4501 gcaaaagaag gaacagaaga ataaattgag ttctataaaa agtgaaaaag actacttgga
     4561 atcggaagag aggaaaaaaa ctcaggagta taattctctg atgcaacaga ttgatgaact
     4621 gaataaggaa attgaaacgg ttgatgaagc tataaaggaa agcgaagaga aatataacta
     4681 tcagatggag ctgttcaagg tcagacttaa agtaatgtat caaaactcta atgttacata
     4741 cattgaatcg ttgtctgagt cggaaagcat tgttgatttc tggaacaggc ttgagatagt
     4801 ttctgctata agtaaaaaag acaaagaact tattgaggac atcaaggcag caaagacaga
     4861 tgtcgagttt aaaaagcagc ttgcagttga gaaaaagact gcggttaagg aaagtgcgga
     4921 tgcgtcttta aacaagttga acgaaattaa ggcgacaaga tccggtctcg acagagaaat
     4981 cagcgacata agttctcagt tgaagaagct tgagcagcag gaggatgagc tgttaagaaa
     5041 gtccaatgaa ctggccaatc aaataaaaag ccttcagcga agcggaagtt atgccggggg
     5101 gagtatgctt tggcctactc cgagctgttc aagaatttca tcctactttg gaaacaggct
     5161 tcatcctata cttaaagtgt acaaaatgca taatggtata gacatttcag cgggtaacgg
     5221 tgcttcgata attgccgcta ataagggagt agtgatagtg gcaggatggc agagcggtta
     5281 cgggaatacg gtgatagttg accatggtgg tgggatttct acgctatatg ctcactgcag
     5341 caagcttctt gtgaaagtgg gcgattcggt aaaagccggt gatacgattg cgaaggttgg
     5401 aagtaccgga ctttccacag gaccgcattt gcattttgag gttagaaaaa acggtacacc
     5461 ggttaacccg ttggattatg taaaaccata aaaaacagtt atatggaata tgttattaat
     5521 atcgccatat ctgaaattaa gctcgtacat tttatggtac gggcttaatt gttagcggga
     5581 tttcacgaag tgaaatgacc aaaaagcgat gggattaaaa tattatttaa agtgcaagca
     5641 tatgatataa cgaggcgata tgccttcggt agggtattga ttaagttcaa caaattttag
     5701 caaggggagt gttctcaaat tgaataaaaa tgtgattacc ggcaaagtat tacccatatt
     5761 gatagcagcg ttactctctt caacaatcac tgcttatggt tttttggttt ggcattataa
     5821 aaatccgaat tatgttgtaa tgtccgagaa ggaggccgag aagtataagg attcaaaggg
     5881 ctttgaccct caatatgcca taacctttga taaagacaat gtcgattctg tgattattaa
     5941 taagtttaat gaggtaaaaa aacttttgga cacttattat tataaaaata tcaatcagaa
     6001 tcagttgctt gagggggcta ttgccggaat gaccgattct ttgaatgacc cctacactgt
     6061 ttattttaca aaagaacaaa tgaaagaatt caacgaaaaa acccagggca gctatgttgg
     6121 aatcggtgtg tccataagca tggataatga tgggatactc acggttattg aggcttttga
     6181 ggattctcct gcaaaggaag taggtatttt gcccggagat aagatagtaa aggttgacga
     6241 taaagatgtt acaactataa gggatgacaa tttaattgtc agcatgatta agggtgagga
     6301 gaatacaaaa gtcaaaatta cagtattcag accttccgag ggaacttatc tggatttcga
     6361 tgttataagg aaggagatta aagttgtaaa cataaacagt gaagtgctgg aggggaatat
     6421 aggttatata agaataataa tgttcg
//
LOCUS       DAOOJC010000087         6410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_9967_length_6410_cov_3.327616, whole genome shotgun sequence.
ACCESSION   DAOOJC010000087 DAOOJC010000000
VERSION     DAOOJC010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9967_length_6410_cov_3.327616"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLA01_03680"
     CDS             <1..224
                     /locus_tag="PLA01_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HPD00433.1"
                     /translation="ADITAIIEEEKLKPEETRRFIDNAFRDGMLKTTGTAIDKIMPPV
                     SRFGGGRAAKKQGIIEKLMYFFEKYLGLV"
     gene            295..1191
                     /locus_tag="PLA01_03685"
     CDS             295..1191
                     /locus_tag="PLA01_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HPD00434.1"
                     /translation="MHKKQKSPFELLRNKAILAILDGDSQFGEIDDVEISMPYLSGPT
                     IVEISQLFGLSETYGWNGGALSRWNYLDNLLKHGIETNRINDILSYLFSKQQFTDKLK
                     RKTPDQIEEIYNYIVKTVIEQINGILYFGGNKLTIINQRFHIIPINSTIKVTTPQVKN
                     IDREYIKDISERAMLDVVNGSYDSAITKSRTLLEEVFCYVIENKNETPTDSGDIGRLY
                     KQVKGLYNMHTDKNLDRRINTLLSGLEKIVSSIAEMRNNNSDAHGVGSQRISISEHHA
                     RLLVNSAMTMAEFILSVGNNTK"
     gene            1557..2054
                     /gene="trmL"
                     /locus_tag="PLA01_03690"
     CDS             1557..2054
                     /gene="trmL"
                     /locus_tag="PLA01_03690"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HPD00435.1"
                     /translation="MGINIVLVEPEIPQNTGNIARTCAATGANLHLVKPLGFSVDDRY
                     LKRAGLDYWSDVKITYYDSFNELREKYKENTFFYATTKAQNSYTEVSYPEDCFIVFGK
                     ETAGLPEELLVRNKEWCIRIPMKSDIRSLNLSNSVAIIVYEALRQNNFIGFNTKGNLT
                     KYSWR"
     gene            2058..3179
                     /locus_tag="PLA01_03695"
     CDS             2058..3179
                     /locus_tag="PLA01_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPD00436.1"
                     /translation="MVNRERLVNEFLELVRIDSLSYKEREMADVLKAKLEGMGLSVEE
                     DGTGAVIGGNAGNIICRIPGKTDVPAILLMAHMDTVEPGIGKSPVVEGNIIKTDGTTV
                     LGGDDAAGIECILEAVRVLKENNLEHGDIYVVFTVAEECGLLGAKNLELKNINARYAF
                     VMDNGGDIGEVAVNAPSQNKIDVKVLGRASHAGLQPEKGISAVQIASEAIANMKLGRI
                     DEETTANIGIIKGGQATNIVCESVDIKAEARSRNVEKLKKQTEHMKNCFERSAQKYGG
                     EVVFNSEMLYPAFNISEDDEIIAILKEASEKTGIPLKLVSTGGGSDTNIINAKGIKAV
                     DVSVGMDKVHSQKEQISIDNMMKAVEFLIAIITSVKNFC"
     gene            3314..4831
                     /locus_tag="PLA01_03700"
     CDS             3314..4831
                     /locus_tag="PLA01_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPD00437.1"
                     /translation="MIQKTGLLNNSIKKLNEQYFSNWYKKAEIILYIIVCIVMGIIQS
                     INAEHDAMINVRGYISHIQSTIVILMAFRYGYFGLLVSSALIATETFFIIRECFVNFD
                     NYLLLGLTLKVFTMLIACFIAVLTHKQQVQNRKLEIMAITDELTGAYNQRFFHMILED
                     GIEKAQKNKCSVGLIMIDVDNFKMYNDIYGHDCGDEILKATTTILKKIVKEESYVFRY
                     GGDEFAVIFRNTDLQHMESMANNLREEFERLKKDYYRDDIYDKVTLSIGLSEYPNMSK
                     SKNELIYQADMALYHAKNLGKDKVHLYQDAILQLRKNISSDHQQLIGIFKGLLGTISA
                     KDKYTHGHCERVSAYGVMIGEALNMSLKEISIIQYAGLLHDIGKIEMPKNILNKKEEL
                     TEEEKNYLKQHPIYSENILEPLNEIELLTDYVRHHHERYDGKGYPDGLAGEEISLGAR
                     ILCVADSFDAMVSERPYSKSMTMEQAFKELEKNAGTQFDPEIVKIFIKVMKARAA"
     gene            5067..6113
                     /locus_tag="PLA01_03705"
     CDS             5067..6113
                     /locus_tag="PLA01_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HPD00438.1"
                     /translation="MNNLYYFPDRKKKAIYRFCKILSTQSFIPDRVVENKEIIENNHM
                     TFNSDAIVKSIGVERRHIAQNNEDDSDLLLKSARGCLRKCGILPDKLSRIIVNKFMGD
                     NLLPMTASKLQSKLGSKTAVHAFDIDGGTSSFIYSVDVASRFINSGDEYILISSGGIH
                     TKFASKSDPRVAFLFGDASASILLGYSQEKHILSCYLYSNHRYIDLAKAISPLSMTSK
                     LEDVMENGLMNTVLDSYKLDNWKIAEDFYREATAKVAENILEESGLGMKDIDLVLVTE
                     NNRKIWELTLETLGVGTEKSISLIRDYGNTMSAMLPLLIDYGLHSGRIEEGMNIMMIS
                     HGEGFSGGGLIYRV"
     gene            6151..>6410
                     /locus_tag="PLA01_03710"
     CDS             6151..>6410
                     /locus_tag="PLA01_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPD00439.1"
                     /translation="MDYDYIEIYNKTKDLIVDYLRVDESEITPNTDLAEDLCVDSIAL
                     AELGFRISEAFSIPMLDPKPEMLVMQNLVSLIMDNMKNGVSQ"
BASE COUNT         2199 a          930 c         1438 g         1843 t
ORIGIN      
        1 aagcggatat tacagcaatt atagaagaag aaaaattaaa gcctgaagaa acccgccgtt
       61 tcatcgacaa tgctttccga gacggaatgc ttaaaacaac cggtacagcc attgataaaa
      121 tcatgccacc tgtatcccgc tttggcggag gcagagctgc gaaaaagcaa ggaattatcg
      181 agaagctgat gtatttcttt gagaagtatt tagggttggt gtagcaaata atcaatacag
      241 cagattaaat aaattgatag gttactatag aataaatatt gtgaggtgaa atgattgcat
      301 aaaaagcaaa aatctccatt tgagctatta agaaataagg ctattctagc tattttagac
      361 ggtgattctc aatttggaga aatagatgat gtagagattt caatgcctta tttaagtggc
      421 ccgactatag tagaaatttc ccaattattt ggactgtctg aaacatacgg atggaatgga
      481 ggagcattaa gtagatggaa ttacttagat aaccttctta agcatggaat tgagactaac
      541 cgaattaatg atattctttc ctatctattt tcaaaacagc aatttacaga taaacttaaa
      601 aggaaaacac cagatcagat tgaagagatc tataattata tcgttaagac agtaatagaa
      661 cagattaatg gaatacttta ttttggtggc aataaactta ctatcatcaa tcaaagattt
      721 catattattc caataaattc tacgattaaa gtaactactc ctcaagtaaa aaacattgat
      781 cgagaataca ttaaagatat ttctgagagg gcaatgcttg atgtggtaaa tggaagttat
      841 gacagtgcaa tcactaaatc tagaacatta ttagaagaag tattttgtta tgttatcgaa
      901 aacaagaatg aaacgccaac agacagtgga gacataggta ggctttataa gcaagtaaaa
      961 gggttataca atatgcacac agataaaaac cttgatagac gaatcaatac actactttca
     1021 ggtttggaaa aaatcgtttc atctattgct gagatgagaa acaataatag tgatgcgcat
     1081 ggtgttggaa gtcaaagaat tagtatatct gagcatcatg ctagacttct tgtaaattct
     1141 gcaatgacta tggctgaatt tatactttca gtgggaaata atacaaaata ataaagagac
     1201 cagccacatc aagccattaa gtttaaatgt gactggtctt ttacttttac cccgcttgaa
     1261 gctgttcatt taagttttct tcaatcccta agtgaacgct tctttaaggg tctttttttt
     1321 atgaaccgag taagtacact gattttttat atattcaccg ccgatatgtg caaagtgaaa
     1381 attaataatg tttctttcat ttttttcaca ttaattatat taaacgctct ttaaataacc
     1441 cggttgagtg tgctaatata aaatttgaaa ataataaaca gaacccgttc agctaaggct
     1501 ggatgcggta gatattgcaa aaataatata ctttgagttg tgaaaaggag taggatatgg
     1561 gtataaatat tgtgcttgta gaaccggaaa tacctcagaa tacaggaaat attgcaagaa
     1621 catgtgcagc cacaggtgca aatttgcatc tggtcaagcc tttgggtttt tcggtggacg
     1681 atagatatct taaaagagca ggtctggact attggagtga tgtaaaaata acgtattatg
     1741 atagttttaa cgaattgagg gaaaaatata aagagaatac ttttttttat gccactacca
     1801 aggctcaaaa ttcatatacc gaagtaagtt atcctgagga ttgttttatt gtattcggga
     1861 aggaaacagc gggtttgccg gaagagctgt tggtaaggaa taaggaatgg tgcatcagaa
     1921 ttccgatgaa atcggacata agatcattga atttgtcaaa ttctgttgct ataattgttt
     1981 atgaggcttt acggcaaaac aactttatcg gatttaatac aaaaggaaac cttacaaaat
     2041 attcatggcg gtgaagtatg gttaatagag aaagattggt aaacgagttt ttggaactgg
     2101 tgagaattga cagcctttcc tataaggaga gggaaatggc ggatgtgctt aaagcaaaat
     2161 tggaaggtat gggtttatcg gttgaggaag acggaaccgg ggcagtaatc ggagggaatg
     2221 ctggtaatat aatatgcagg atacctggga agaccgatgt gccggctatt cttttgatgg
     2281 cacatatgga cacggtggag ccgggtatcg ggaaaagtcc tgtggttgaa ggaaacataa
     2341 taaaaactga cggaacaacg gtacttggtg gagatgatgc cgcggggata gagtgcatat
     2401 tggaagctgt cagagttctt aaggaaaaca atctggagca tggagatatt tatgttgtgt
     2461 ttaccgtggc agaggagtgt ggattgctgg gagcaaagaa cctcgaattg aaaaatatca
     2521 atgcccggta cgcttttgtt atggataacg gcggagatat tggcgaagtt gcagtaaatg
     2581 ctccgtcgca aaacaagatc gatgtaaaag tactggggcg ggcttctcac gcaggacttc
     2641 agcctgagaa ggggattagt gccgttcaga ttgcatcgga ggcaattgca aatatgaaac
     2701 tgggcagaat tgatgaagag actacagcaa acattgggat aattaagggc ggtcaggcaa
     2761 caaacatagt ttgtgaaagt gttgatataa aggccgaggc cagaagcaga aacgtggaaa
     2821 aactaaaaaa acaaacggag cacatgaaga actgctttga aagatccgca caaaaatacg
     2881 gtggagaggt tgtgtttaat tcggaaatgc tctatcctgc tttcaacatt agtgaggatg
     2941 atgaaattat agcgatttta aaagaagctt ccgagaaaac agggattcct cttaaattgg
     3001 tatccaccgg gggaggaagc gacacaaata taatcaatgc aaagggtata aaggctgtgg
     3061 atgtcagtgt cggaatggat aaggtgcaca gtcagaaaga gcagatatcc atagataata
     3121 tgatgaaagc tgtcgaattt ttaattgcaa taattacttc tgtaaagaac ttctgttaag
     3181 aattttttta aacctttgta acctatgtat ggaaatatta ataatactga agtataatat
     3241 attggagtat acctttgata gttaaaagtg taataagatt atagacatct attgtatggg
     3301 tggttggatg tatatgattc agaagacagg cttgttgaat aatagcatta aaaaattaaa
     3361 tgagcaatac ttcagtaact ggtataaaaa agctgaaatc attttataca ttatagtctg
     3421 cattgtaatg ggcattatac aaagcattaa tgcagagcat gatgctatga ttaatgttcg
     3481 gggttatatc agccacatac aatcaacgat tgtaatcctt atggcatttc gttatggtta
     3541 ttttgggctt ttggtatcgt ctgcattaat tgcaaccgag acatttttta taataaggga
     3601 gtgctttgtg aactttgata attacctatt gctggggttg actttgaagg tgttcaccat
     3661 gttgattgcg tgtttcattg cagttcttac acacaagcag caagttcaaa acagaaagct
     3721 tgagataatg gcaattaccg atgaattgac cggtgcatac aatcagaggt tttttcatat
     3781 gatacttgag gacggaatcg aaaaagctca gaaaaacaaa tgttccgttg gacttattat
     3841 gatagatgtc gacaacttta aaatgtataa tgacatatac gggcatgatt gtggagatga
     3901 aatattaaaa gcgaccacaa cgatattgaa aaaaatagtt aaagaagaaa gttacgtatt
     3961 cagatacggt ggggatgaat tcgcagttat cttcagaaac acagatttgc agcatatgga
     4021 gtctatggca aataatcttc gggaagagtt tgaaaggtta aaaaaggact actacaggga
     4081 tgacatatat gacaaggtta cattatctat tggtttgtcc gagtatccca acatgtcgaa
     4141 aagtaaaaat gagcttatct atcaagcaga tatggcatta tatcacgcaa agaatctggg
     4201 taaggataag gttcatctct atcaggatgc gatattgcag ctcagaaaaa atatcagttc
     4261 ggatcatcag cagcttatcg gaatatttaa gggcttgtta ggtactattt ctgcgaaaga
     4321 caaatatacc catggacatt gtgaacgggt ttccgcttac ggagtaatga taggagaagc
     4381 tctcaatatg agcttgaaag aaataagcat aattcagtat gccgggttat tgcacgatat
     4441 cgggaaaata gaaatgccca aaaacatact taacaagaaa gaggagctga cggaagaaga
     4501 gaaaaattat ttaaaacagc atccgatata tagtgagaat attcttgaac ctttgaatga
     4561 aatagagtta cttacggact atgtaagaca tcatcacgaa aggtatgacg gaaaaggcta
     4621 tccggacgga ctggcaggag aagagattag ccttggtgca aggatactgt gcgtagctga
     4681 ttcctttgat gcaatggtat ccgaacgtcc ttacagtaag agcatgacta tggaacaggc
     4741 attcaaagag cttgaaaaga atgccggaac ccaatttgat ccggaaattg tcaagatatt
     4801 tattaaggta atgaaagcaa gagcagcctg aaatatttgg aatacagaaa gttaattaca
     4861 ctcatggcgt tgccttccgt tttcttgtca tatcaaatat tatatacaaa aattatgccg
     4921 gaatattgca aaaaatataa gttaatgata taatatttgt aatattaata caacatataa
     4981 atttcttaga aataatgatt tgttttttcg tgcggagcag cttatttatg ttataagaca
     5041 cattttgacg gacttagaga ggttctatga acaacttata ttattttccg gatagaaaga
     5101 aaaaggcaat ttataggttt tgcaaaatac tatctacaca gtcttttatt cctgatagag
     5161 ttgttgaaaa taaagaaata attgaaaaca atcacatgac ttttaacagt gatgcaattg
     5221 taaagtctat tggtgtagaa agaaggcata ttgcacaaaa caatgaggat gacagcgacc
     5281 tgcttttaaa atcggcaagg ggatgcctca gaaaatgcgg tattttgccg gacaaactgt
     5341 ccagaattat tgtaaacaag ttcatgggag ataatttgtt gccgatgacg gcaagcaagc
     5401 tgcagagcaa gttgggaagc aaaactgcgg tacatgcctt cgatattgac gggggaactt
     5461 catcctttat ttattctgtt gatgtggctt caagatttat aaattcgggt gatgagtata
     5521 ttcttatttc atccggtggt attcacacaa aatttgcaag caagtcggat ccgagggttg
     5581 cctttttgtt tggggatgct tcagcttcga tacttttagg gtactcacag gaaaaacaca
     5641 tattatcgtg ttatttgtat tcaaatcaca ggtatattga tctggcaaaa gcaataagtc
     5701 ctttatctat gacatcaaag cttgaagacg taatggaaaa cggtttgatg aatacagtgc
     5761 tggatagcta taaattggat aactggaaaa ttgctgagga cttttacagg gaggcaaccg
     5821 cgaaggtggc cgaaaatatc cttgaagaaa gcggccttgg catgaaggat attgatttgg
     5881 tattagttac ggaaaacaat agaaagatat gggagttaac tttggagacc ttgggggttg
     5941 gcaccgaaaa aagcatttcc ttgataagag actatggaaa cactatgtcg gcaatgcttc
     6001 ctttgctcat tgattacggg ttgcattccg gaaggataga ggaggggatg aatattatga
     6061 tgatatccca tggagaggga ttcagcggtg gaggcttgat ctacagggtg taatcgtaac
     6121 cttgcatttt ccgatgggga gggtgggatg atggattatg attatattga aatctataac
     6181 aaaacaaaag atttaatagt tgattattta agagtggatg aaagtgaaat aacaccaaac
     6241 acagaccttg ctgaggatct ttgtgtggat tccatcgctc ttgctgagtt gggcttcagg
     6301 atttctgagg ctttttcaat accaatgctt gacccgaaac ccgagatgct tgtaatgcag
     6361 aatcttgtca gtttaataat ggacaatatg aagaacggtg tttcacaata
//
LOCUS       DAOOJC010000088         6405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_9978_length_6405_cov_3.550236, whole genome shotgun sequence.
ACCESSION   DAOOJC010000088 DAOOJC010000000
VERSION     DAOOJC010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9978_length_6405_cov_3.550236"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1762)
                     /gene="brxC"
                     /locus_tag="PLA01_03715"
     CDS             complement(<1..1762)
                     /gene="brxC"
                     /locus_tag="PLA01_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004059180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HPD00440.1"
                     /translation="MLLKDMFAKPIDRDIKGVIKVGQSDNENIRQELEEYVVTRELQK
                     HFSQFFSSYKKGINGYTDKMGVWISGFFGSGKSHFLKILSYLLKNTEVNGKKAIDYCI
                     DDKKIVDPMVLADMRLAASIAADTDVVLFNIDSKSEITGKQSKDAIVSVFLKVFNEMQ
                     GFCGSIPFLADLERALVEVGRYEEFKKAFEENYGKSWESSRHKFDFIQDNIVEVLVGI
                     GFMSESAARNWCEKATETYNISIENFAKLVKEYIDRKGPNHHVVFLVDEIGQYIGEDS
                     KLMLNLQTVTEDLGTACRGKAWVVVTSQQDIDAIAKDMGLRSNDFSKIQGRFDTRLSL
                     SSANVDEVIKKRILEKKPTAEQTLRLMYEQKATIIKNLIVFNDSAEKKLYSGDADFAS
                     VYPFVPYQFNLLASVLTSIRTHGASGKHLSEGERSMIALFKESAMKLMDRENGAIIPF
                     NVFYDALHQFLDHSHKGVIIRAHDNNIINPDKEAECFNVNVLKTLFMIKYVKEITSNV
                     ENITSLMIDDINTDRIELKKKVEEALKVLVNQTLVQKNGDIYTFLTDEEQEINREIDR
                     QNVEMAEIINKVSEMVFIDIL"
     gene            complement(1777..2355)
                     /locus_tag="PLA01_03720"
     CDS             complement(1777..2355)
                     /locus_tag="PLA01_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HPD00441.1"
                     /translation="MASLEERLDAVERMIKEANFRENKGLGNEVGYYVFDYPPEKELY
                     VRERIEYMITRNTRGTEGFELVVFDLYDIIIDLLEEEGFLEQCFKFEKKDGMERIIKA
                     VGNLLQFNDDENRVVKYIQDNTPKDAVVFLTGVGKCYPILRSHKVLNNLHQAIDRVPV
                     VMFFPGKYDGQELVLFSQIKDGNYYRAFRLVD"
     gene            complement(2355..2957)
                     /locus_tag="PLA01_03725"
     CDS             complement(2355..2957)
                     /locus_tag="PLA01_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HPD00442.1"
                     /translation="MVRKEYSAGMVKLSFWFLEFRKVIELLNSGKTINEIKEMNIKEN
                     IFAAPTMARAIQIFNTVSDRVMSLDESFYKLFEKSDIATQKIIAIIAVMNVDSLFFDF
                     VYEVFREKLIIGNYTLSDSDIRSFFKDKQLQSEKVAGWTDYTLKRLGMCYKTMLMEAG
                     IIDRATGDRRILKPIIDVALEKCMKDHGMELFIHALTGVR"
     gene            complement(2982..3212)
                     /locus_tag="PLA01_03730"
     CDS             complement(2982..3212)
                     /locus_tag="PLA01_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019189322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPD00443.1"
                     /translation="MNVCYNKLFKLLIDKGMKKTDLRLATGISPNTLSKLSNNEYVSM
                     EVLVKICRVLHCDVGDIMEVVITDSKDVIATK"
     gene            complement(3590..4054)
                     /locus_tag="PLA01_03735"
     CDS             complement(3590..4054)
                     /locus_tag="PLA01_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00444.1"
                     /translation="MEQVLNQILTELKGLNNKVDNLEKGQEQLIEKQGQFEKKLDQFE
                     KKQGQFEKKLDQFEKKQGQFEKKLDQFENKLGQFEKKQEQFAKTQEGFAERLDRIEKK
                     LDAVYEQTAELTEFRTETRESLNVINNSIRFLLHKETENEREIFLLKEKKAK"
     gene            complement(4297..5046)
                     /locus_tag="PLA01_03740"
     CDS             complement(4297..5046)
                     /locus_tag="PLA01_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPD00445.1"
                     /translation="MKLEKIIAVRTDKTVYRDGDKAIKVFSSNYSKANVLNESLNQAR
                     VEETGLNIPKLLEVTKIEDKWAIVTEFIEGKTLEQLMKDNPHKYDEYLNLFVDIQLEV
                     LSKKAPLLNKLKDKMHRKISQTGLDATTRYELHTRLGSMPEHDKVCHGDFNPSNIIIS
                     KDNVPYILDWSHATQGNASADAARTYLLFWLAGESDRADKYLNLFCKKSDTAKQYVQK
                     WISIVAASQLVKGKEAERELLLHWIDVVDYE"
     gene            complement(5046..>6405)
                     /locus_tag="PLA01_03745"
     CDS             complement(5046..>6405)
                     /locus_tag="PLA01_03745"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPD00446.1"
                     /translation="PSLTLGCGSWGGNSVSDNVGVKHLLNIKTVAERRENMLWFRTPE
                     KVYIKRGCLPVALDELKNVMDKKKVFIVTDTFLYNNGYTKPVTDKLDEMGIVHKTFFD
                     VAPDPSLACAKAGAAEMAAFQPDTIIALGGGSAMDAAKIMWVMYEHPEADFMDMAMRF
                     ADIRKRVYTFPKMGEKAYFIAIPTTAGTGSEVTPFAVITDEKTGVKYPLADYELLPDM
                     AIVDADMMMNAPKGLTSASGIDALTHSLEAYASMLATDYTDSLALRSIKMIFEYLPRA
                     YDNGSKDPVAREKMADAATMAGMAFANAFLGVCHSMAHKLGAFFHLPHGVANALMIDE
                     VIRFNAVEVPVKMGTFPQYGHPHTLARYAEIADYLGVKGKNNEEKLENLIKAIDELKE
                     KVGIKKTIKDYGIDEKDFLDRLDEMVEQAFDDQCTSANPRYPLMSEIKEMYLNAYYGG
                     AK"
BASE COUNT         1804 a         1496 c          986 g         2119 t
ORIGIN      
        1 ttaaaatatc aataaaaacc atctcggaaa ccttgtttat aatttcagcc atttcaacat
       61 tctgtctgtc aatttcacgg ttgatttcct gttcctcatc agtcaggaaa gtgtatatat
      121 caccgttttt ctgcactagg gtctggttta caagaacttt taaggcttcc tccaccttct
      181 ttttcaattc aatacggtct gtattaatat catctatcat caggcttgta atattctcaa
      241 cattggaagt aatctccttg acatacttaa tcatgaacaa ggtttttaat acgttcacgt
      301 tgaagcattc tgcctctttg tcgggattga taatattatt gtcatgtgca cggattataa
      361 cgcccttatg gctatgatct aaaaattggt gcagtgcatc ataaaacaca ttgaatggta
      421 ttatagcacc gttttctctg tccattagct tcatggccga ttccttgaat agtgcaatca
      481 tagaacgctc accttctgac aaatgcttac ctgatgctcc atgggttctg atagaagtca
      541 acacgcttgc caaaagatta aattggtaag gtacaaaggg ataaaccgaa gcaaaatctg
      601 catccccaga gtatagcttt ttctctgcac tatcgttgaa aacaatcaaa ttctttataa
      661 tagtcgcttt ttgctcatac ataagtctta atgtctgttc tgcagtaggt ttcttttcta
      721 aaatcctttt ctttataact tcatcaacat tagcagaaga caaagaaaga cgggtgtcaa
      781 aacgaccttg tattttggaa aaatcattac tgcgaagtcc catatcctta gctatcgcat
      841 caatatcctg ttggctagtg actactaccc atgctttgcc ccgacatgca gtacccaaat
      901 cttccgttac cgtttgaaga ttcagcatta atttagagtc ttctcctatg tactgcccga
      961 tttcatcaac taagaatacc acatgatgat tagggccttt acggtcaata tattccttca
     1021 ccagctttgc aaaattctca atgctgatgt tataggtctc agtcgctttc tcacaccagt
     1081 tgcgggctgc tgattcactc ataaagccta taccgacaag cacttcaaca atgttgtcct
     1141 gaataaaatc aaacttatga cgtgaggatt cccatgactt accgtagttt tcttcaaagg
     1201 cttttttaaa ttcttcataa cgcccaacct ctacaagagc acgctccaaa tcggcaagaa
     1261 acggaataga accacagaag ccctgcattt cattgaatac ctttaaaaat acagatacaa
     1321 tggcatcctt tgattgtttg cctgttattt cgctctttga atcaatatta aacaacacta
     1381 catccgtatc tgctgcaatg ctggcagcca acctcatatc agcaagtacc atcgggtcaa
     1441 caatcttttt atcatctata caataatcga tagctttttt accgtttacc tctgtgttct
     1501 taagtaaata ggacagaatt ttgaggaagt gagatttacc gcttccaaaa aagccagata
     1561 tccaaacccc cattttatcg gtatagccgt ttataccctt tttgtagctg gaaaagaact
     1621 gtgaaaaatg cttttgaagc tctctggtga caacatattc ttccagctct tgcctgatat
     1681 tttcattatc gctctgtcct accttgataa ctccttttat gtctctatca attggcttgg
     1741 caaacatatc tttgagtaac atagctttcc tcccctttag tccacaagcc gaaaggctct
     1801 gtagtaatta ccgtctttta tctgtgaaaa cagtactaat tcctgtccat catacttacc
     1861 tgggaaaaac attaccaccg gcacccggtc aatagcttga tgaaggttat tgagcacctt
     1921 atgagatctc aatataggat agcatttacc aacgcctgta agaaacacaa ccgcatcctt
     1981 tggtgtatta tcctgaatgt atttaacaac ccggttctca tcgtcattga attgcaatag
     2041 attgcccacc gcttttatta tacgttccat tccatctttc ttttcaaact taaaacattg
     2101 ttccaagaat ccttcctctt ctaaaaggtc aattataata tcatacaaat caaatacaac
     2161 gagctcaaaa ccttccgttc ccctggtatt tctggttatc atatattcaa ttctttccct
     2221 gacatacaat tctttttccg gcggataatc aaatacataa taaccgacct catttcccag
     2281 tcctttattt tcacggaaat tagcctcttt tatcatgcgt tccaccgcat ctagccgttc
     2341 ctccaaactc gccattacct cacccctgta agtgcatgga taaagagctc catgccatga
     2401 tctttcatac atttttctaa agcaacatct ataattggct ttaatattct cctgtctcct
     2461 gttgctctat caataatacc ggcttccatg agcatggtct tgtaacacat tcctagcctt
     2521 tttaaagtat agtcggtcca gccagccacc ttttcactct gcaattgctt gtccttaaaa
     2581 aaactacgaa tatcactgtc cgataatgta taattaccta taatcaactt ttcacgaaat
     2641 acctcatata caaaatcaaa aaacagactg tcaacattca taaccgctat aattgcaata
     2701 attttctgtg ttgcaatatc acttttctca aacagcttgt agaagctttc atcaagactc
     2761 attactcgat cggatactgt gttgaatatc tgaatagctc tagccattgt cggtgctgca
     2821 aatatattct ctttaatatt catctcttta atctcattta ttgtctttcc ggaattcaaa
     2881 agttctatta ctttcctaaa ctccaaaaac caaaatgaaa gcttaaccat tcctgcacta
     2941 tattcttttc ttaccaccta tattcctcca attaattttc tctatttagt agctattaca
     3001 tccttgctat cagttatcac tacttccata atatctccga catcacaatg gagtacccgg
     3061 catattttca ccagtacttc catagataca tattcattat ttgataactt tgacaatgta
     3121 ttaggactta ttccggtagc taaccgcaaa tcagtctttt tcattccctt atcaatcaat
     3181 aacttaaata gcttgttata gcatacgttc attgataaac tcctctgttt tttagttttt
     3241 aatagataac cgataaatat tatatcacat ttaatattac aataaccgta aaaaaatata
     3301 gattcatgcg aatctataac tagaaataat atatgaaata taaatttgtg aattattata
     3361 aaatttacac caatgcgaat aatttttgaa cattcgttat ctcttttttc tgagaccaaa
     3421 ttgtgaattg ttcaactcaa aaggtagtag gttctttcct ccaacaaaat aaattgaaat
     3481 ttatttttac caagattggg attcagtttt gatttatgaa cacaaagata aagaaatcac
     3541 taaaaaagaa gatcaaatta agcaaaataa ccgagattag aatactcggt tattttgcct
     3601 tcttctcttt caacaagaat atttctctct cgttttctgt ttctttatga agaagaaaac
     3661 gaatactgtt atttataaca tttaaacttt ctctggtttc agttcgaaac tctgtcaatt
     3721 cagcagtttg ctcatatact gcatctaatt tcttttctat ccgatccaga cgttctgcaa
     3781 atccttcctg agtttttgcg aactgttcct gcttcttttc aaactgcccc aacttatttt
     3841 caaactggtc cagcttcttt tcaaactgcc cctgcttttt ttcaaactgg tccagcttct
     3901 tttcaaactg cccctgcttt ttttcaaact ggtccagctt cttttcaaac tgcccctgct
     3961 tctctatgag ttgctcttga cccttttcaa ggttgtcaac tttattattg agccccttta
     4021 attctgtaag aatttgattt aaaacctgtt ccataatatt acctctatag aaatattaat
     4081 acacgactcg gtgctatata ttattatact atatttaccc tataaatagt caagttaatt
     4141 accataatat tcttttaaca ggggatcaaa gcaccttcgc tcaaatacac actttttttg
     4201 atgctgccta aattgaaaga ctcctcataa ggggagtctt tcaattaaag ggtataatct
     4261 ttctatgcat atagaaacgt acctctaaag aacctgctat tcataatcta caacatctat
     4321 ccaatgcagt aataattccc tttctgcctc cttgcctttt acaagctgcg atgcagctac
     4381 aattgaaatc cacttctgaa cgtactgttt tgcagtgtca ctctttttgc agaacaaatt
     4441 caaatactta tctgctctat ccgactcacc tgcaagccag aaaagaaggt atgtccttgc
     4501 agcatcggca ctggcattcc cctgtgtagc atgggaccag tccaggatat agggcacatt
     4561 atctttggag atgataatat tgctcgggtt aaaatcacca tggcacacct tgtcgtgttc
     4621 gggcatgctc ccaagccttg tatgaagctc atagcgtgtt gtagcatcaa gcccggtttg
     4681 agaaatcttt ctgtgcatct tatccttgag cttgttcaat aaaggtgcct ttttgcttaa
     4741 gacctccagt tggatatcca caaacaggtt caggtactca tcatacttgt gaggattatc
     4801 cttcataagc tgctctaatg tcttgccttc aatgaactcc gtcacaattg cccatttgtc
     4861 ctcaattttc gtaacctcca gaagtttggg aatattaagc ccggtttctt ccaccctggc
     4921 ctggtttaat gattcattca gcacatttgc ttttgaatag ttgctgctaa agacctttat
     4981 tgccttatcc ccatccctgt aaaccgtttt atccgttctt accgctataa tcttttcaag
     5041 cttcattatt ttgcacctcc gtaataagca ttaagataca tttctttgat ttcactcata
     5101 aggggatacc tcggatttgc actggtgcac tggtcatcaa aggcctgttc caccatttca
     5161 tccagtctgt ccaggaaatc cttttcatct atgccgtaat ccttaatagt cttctttatt
     5221 cccacctttt ctttgagctc atcaatagct ttaatcaagt tttcaagctt ttcttcatta
     5281 ttcttgccct ttacgccaag gtaatctgca atttcagcgt agcgtgccag agtatgggga
     5341 tgaccgtact gcgggaaagt acccattttt accggaacct ccactgcatt aaaccttatt
     5401 acctcatcta tcataagagc attggcaact ccatggggca agtggaagaa agcaccaagc
     5461 ttgtgtgcca ttgagtggca tactcccaag aacgcattgg caaaagccat acctgccatt
     5521 gttgcagcat cagccatttt ttctctcgca accggatcct tcgagccgtt gtcatacgcc
     5581 cggggaaggt attcaaatat catcttaatt gaacgaaggg ccaagctgtc ggtgtaatcg
     5641 gtagcaagca ttgaagcata tgcctccaac gaatgggtca gggcatctat accggatgca
     5701 ctggtaagcc ctttgggtgc attcatcatc atatcggcat ctacgatagc catatccggc
     5761 agcagttcat agtccgcaag gggatatttt accccggtct tttcatcggt tataaccgca
     5821 aaaggagtta cctccgagcc tgtacctgcc gtcgtgggaa ttgcaataaa gtatgccttc
     5881 tcacccatct tcgggaaggt gtaaaccctc tttctgatat ccgcaaatct cattgccata
     5941 tccataaaat cggcttccgg gtgttcatac attacccaca tgattttagc tgcatccata
     6001 gcagatccgc cgcccaatgc aattatggta tccggctgga aagctgccat ttcagccgca
     6061 ccggccttgg cacatgcaag ggacggatcg ggagccacat caaagaatgt cttgtgtaca
     6121 attcccattt cgtccagctt atccgttacc ggtttggtat agccgttgtt gtacaaaaac
     6181 gtatcagtta caataaatac tttctttttg tccattacat ttttaagctc atccaatgca
     6241 acaggcaggc aacctctttt aatatatacc ttttcaggcg ttctgaacca aagcatgttt
     6301 tctctcctct cggcaactgt tttaatgttt agcaaatgct taaccccgac attgtcggaa
     6361 accgaatttc ctccccacga accgcagccg agggtaaggg acgga
//
LOCUS       DAOOJC010000089         6402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_9984_length_6402_cov_4.253663, whole genome shotgun sequence.
ACCESSION   DAOOJC010000089 DAOOJC010000000
VERSION     DAOOJC010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9984_length_6402_cov_4.253663"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..1064
                     /locus_tag="PLA01_03750"
     CDS             312..1064
                     /locus_tag="PLA01_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00447.1"
                     /translation="MNLLNIPNIPKKRVRLALVDGRISSGLEKGFSDRGIEIIKTEAH
                     PGLYPAISCHPDAVFHHLGDNLMVYAPGTSMAVVAALEDMGFLLIEGEKPLTAKYPGS
                     VHYNAARVGNLAFHNTRYTDRILRENLCKRGVELVHVNQGYTKCAISVVDENSIITID
                     RGIAKAAERKGLDVLVIEEDGILLPGIKSGFIGGATGLIDEKKLAVAGNIYRLKSAMK
                     ILDFLSQKGIEHVSLSDEPVMDIGSIIPLLTD"
     gene            1305..2222
                     /gene="ytxC"
                     /locus_tag="PLA01_03755"
     CDS             1305..2222
                     /gene="ytxC"
                     /locus_tag="PLA01_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HPD00448.1"
                     /translation="MQFLCIGVNESAGRLIECLKNELNSNNRLCKKYRIKDVTDSEGS
                     TSIECHIEEDNEHSDICFNLYNEFVGNVSDALANYIIDEYEEKLILRIINSNYCYFNS
                     VEKKEIQRIALGIIKNEERNFLNSLFQIKRRNIIVKKLLEYFEHSNSVILDGFVNFRL
                     KDYVKDLEEIVDKAVDDFLMEREYKEFIRLLRYFVDIQEPKFNVIHIIAGYDNKYILL
                     DENKQEITNECLQEFMNDLPEGEINYDDLLVSSLITMAPRRLIIHGTKGFRNKELLET
                     IKNVFWGKLIVCSECELCIINCVRSENKS"
     gene            3048..3887
                     /locus_tag="PLA01_03760"
     CDS             3048..3887
                     /locus_tag="PLA01_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HPD00449.1"
                     /translation="MKVIGFVGPSGTGKSHRAVWVARERGIEYIIDDGLLIKGNQIIA
                     GTSAKKEKTKIGSIKCALFTNDDHANDVKSAVMDNNPESILILGTSDGMVEAIAKRLE
                     FPEVSEKIYIEEVATEFEIKQALSTRKEQGKHVIPVPTFEIKKDFSGYFLDPLQIFRR
                     KGKGRFQLVGEKSVVRPTFSYMGNYTISDYTIYQIVEYVATNIEGVNKVSRFRAENHT
                     EGIYIEMDLVLVYGYRIRPLLREVQRKVIDEVERLTALNIKRLNITAKSLVLDNQKTP
                     NVI"
     gene            complement(3930..4706)
                     /locus_tag="PLA01_03765"
     CDS             complement(3930..4706)
                     /locus_tag="PLA01_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HPD00450.1"
                     /translation="MKEKIYTIPVTDAFKTECECPMCVLEKKLEDENIEYILGPFLME
                     PEGRIQTNESGFCRRHFELLYNSQVNRLGLGLIVDTHMQEQNSELNKLYESKKNLLKK
                     DAEASLMKNITNKLSSKQTETQKFVDELINKLDNLDNSCTICSRISYTMDRYIDVILY
                     LYFKEEDFKAMFDRQNGFCLKHFRLLLEGTKKYLNSKETAIFVDDLISIQVRNLERIQ
                     QEVNWFTQKFDYRNNDAPWGNSKDALIRSIQKLVSYCSLK"
     gene            4916..5803
                     /locus_tag="PLA01_03770"
     CDS             4916..5803
                     /locus_tag="PLA01_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HPD00451.1"
                     /translation="MEYKGYKIMEERNRVIVENVNDFNPLHIFECGQCFRWLRQPDGS
                     YTGVAKGRVINVSYNDGRLELYNTGIEEFKALWFDYFDLGRDYSLVKEKVMKDEVMKK
                     AVSFGSGIRILKQDIWETLISFITSANNRIPMIMKTVNAISEAYGEEIEMDKKKYYAF
                     PKCSEISRSTAGKLELCGGGFRCKYILATSKMIDEGEMSLDALAFMETKDAREYLKRF
                     PGVGPKVADCTLLYSGTKYDVFPTDVWVKRVMEELYFKRETGFAEIQNFARDYFGEYA
                     GFAQQYLFYYARENKIGAK"
     gene            6028..6241
                     /gene="ssrS"
                     /locus_tag="PLA01_03775"
     ncRNA           6028..6241
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLA01_03775"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT         2076 a          981 c         1412 g         1933 t
ORIGIN      
        1 gaggtgcggt ttatttaaat aaccaccgga tacaaaagta accggcagct agtagctgac
       61 aaatcattgt ttcccgatca cacgctttta ttattaaaat agcttgtcgt caatatattt
      121 tacgcactgt ttgtctgaaa tagtatataa ataaatttaa tgaacaatat aatgttttgc
      181 ttagaaatta tgataaaatt attttctaaa gataatatat attatgaaca taaaagcaag
      241 aatgtgataa cttatgaaaa ttaattttat gtacatcaag ggagtaaaat aaaattatgg
      301 ccggggagaa aatgaactta ttgaatatac ccaatatacc caaaaaaaga gtacggcttg
      361 cactggtgga cgggagaatc agcagcggcc ttgaaaaggg cttttcggac aggggcattg
      421 agattataaa gaccgaggcc catccgggat tgtacccggc tatttcctgt catcccgatg
      481 ctgtgtttca tcacttgggt gataacctga tggtatatgc accgggaaca agcatggctg
      541 ttgttgctgc acttgaggat atgggatttt tattgattga gggagaaaaa ccccttacgg
      601 caaaatatcc cggaagtgtt cattataatg cggcgagggt gggaaacctt gcatttcaca
      661 atacccggta caccgacaga attctcaggg agaacttgtg caaacgcggc gtggaactgg
      721 tacatgtaaa tcaaggctac accaaatgtg caatatcggt tgttgatgag aactcgatta
      781 taactattga cagaggaatt gctaaggcag cggagagaaa gggcctcgat gtgctggtta
      841 tagaggagga cggcatactg ttgccgggaa taaaaagcgg ttttatcgga ggggccacag
      901 gccttattga tgaaaaaaag ttggcggttg caggaaatat atacaggctg aaatcagcaa
      961 tgaaaattct tgattttcta tctcaaaaag gcattgaaca tgtctcactt tctgatgaac
     1021 ctgtgatgga tattggctca attataccac ttctgaccga ctgacaaata ttttcaaaca
     1081 taatccttcg ttgtaactta ctatacttat cagcatataa tatactataa aaatttgaat
     1141 tattacgcag taaaagtgta tgagcaagga tatgctttgc ccggtgggta atttagttat
     1201 gaagaactgg aaattaagaa aattatgcca aaaattgtat atatcgctta ctgcatacaa
     1261 caaattgcta agacatatac tacgtttgaa gggatgtgaa gttgatgcag tttctttgta
     1321 ttggggttaa tgagagtgcc ggcagactaa tagaatgctt gaaaaatgaa cttaactcaa
     1381 ataacaggtt gtgcaaaaaa tatagaatta aagatgttac tgattcggaa ggctcaactt
     1441 caattgaatg ccatattgaa gaagacaatg aacattcaga tatatgtttc aatttataca
     1501 acgaatttgt gggaaatgta tctgatgctc tggcaaatta tataattgat gaatatgaag
     1561 aaaaattgat attgaggata ataaacagca actattgcta ttttaattcg gtggagaaga
     1621 aagaaataca aaggattgct ctcggaatta taaaaaatga agaaagaaat tttttgaaca
     1681 gtttgtttca gataaaaagg agaaatatca tagtaaaaaa gctgttggag tattttgagc
     1741 attccaacag tgttatactc gatggctttg tcaatttccg tttaaaagac tatgtgaagg
     1801 atctggaaga gatagttgac aaggctgtgg atgattttct tatggagagg gaatacaaag
     1861 aattcataag acttttgagg tattttgtag atatacagga accgaagttt aatgtgattc
     1921 atattattgc aggctacgac aacaagtata tattgctcga tgagaacaaa caggaaatca
     1981 caaacgagtg tcttcaggaa tttatgaatg atctccctga aggggaaatc aattatgatg
     2041 accttttagt gagttcatta atcaccatgg cacctagaag gcttattatc catggcacca
     2101 agggctttag aaacaaagaa cttttagaga caattaaaaa tgttttttgg ggaaagctta
     2161 ttgtgtgctc agaatgtgag ttgtgtataa ttaattgcgt caggagcgaa aataaaagtt
     2221 gaagttggaa gtattcgaca agaggtggga aagcccggta taaggggatc ccacttttct
     2281 ttatctttgt tcttgcggta caacaataaa aaataggact aattgcctat atcaattttt
     2341 atccatatat cttgacaata taaaatcaaa tgatacaata tattgtgtaa tacaatataa
     2401 ggtatatttc ttaaggttgc tgtacataat atagaggtat acaaatctga tagggtatta
     2461 ccgcatgctt gtgggtttat cccttgataa agcagtagtt tagtaagtcc acttgtaagt
     2521 taatgaggtt gcttttaaga aggttgcagg attgtaattc gctatgtaaa aaaataacta
     2581 taagttttaa ttctttgtac taatgatgaa aaaacttaaa agcaaaagac gagaaagaaa
     2641 aaaactaaag ataagtgtcc gagaaaggaa agagagtgat tactctcttt tctttaatta
     2701 gtgggaattc actaagtgaa atcctgccaa attatttagt gaagaaaagt gtggaagaaa
     2761 cattgtgaag caaattgtag cccccggaga ttttgttagc aggatttcac ggtggtgtat
     2821 atgcttggaa atatcggctt aaaactgcgg ttttattgta ataccctatg cttttgtgat
     2881 aaaattatat ctactgaaaa agcttagggg ctttgtagct gtttcggttt ttgaaggtat
     2941 cattctatga aacatatcat taaagaaaat ttttacagac gaaagatttt ctttgaactg
     3001 aagtgttata aagaagcaaa gaagtaagcg gtttatggag gtatatcttg aaggttattg
     3061 gatttgtagg acccagtgga accggtaaaa gccatcgggc ggtttgggtt gcgagggaac
     3121 ggggaataga atatataatt gatgacgggc ttcttataaa gggaaatcag ataattgccg
     3181 ggacatctgc caaaaaggaa aagaccaaaa taggttcaat taagtgtgct ctttttacaa
     3241 atgacgacca tgccaatgat gtaaaaagtg cagttatgga taataaccct gaatcgattc
     3301 ttattctggg tacatccgac ggaatggttg aagcaatagc caagcgtctg gaatttcctg
     3361 aggtaagcga aaaaatatat atagaagagg ttgcaacgga gtttgaaata aagcaggccc
     3421 tttcaacaag aaaggagcag ggaaagcatg ttattcctgt gcctaccttt gagataaaaa
     3481 aggatttttc aggatacttt ctcgatcctt tgcaaatatt tcgaaggaag ggaaagggaa
     3541 gattccagct tgtcggtgaa aaatctgttg tacggcctac ttttagctat atggggaact
     3601 atacaatttc cgactacaca atataccaga ttgtggagta tgttgcaaca aacatagagg
     3661 gtgtcaataa ggtgtcaaga ttcagggcgg agaaccatac cgagggcata tatattgaga
     3721 tggaccttgt tctggtgtat ggataccgca taagacctct tttgagagag gtccagagaa
     3781 aggttatcga cgaagttgaa aggcttacgg cattgaatat caaaaggctg aatattactg
     3841 caaaaagcct tgtacttgat aatcagaaaa cgccgaatgt tatttgaaaa tgccctaagc
     3901 cccggttaca ttttctatga gaataaagct tatttcaagc tgcaatagct caccagtttc
     3961 tgaatacttc tgatcagagc atcctttgaa ttgccccagg gggcatcatt gttgcggtag
     4021 tcaaactttt gggtaaacca gttgacttcc tgctggatgc gttccagatt ccgtacctgt
     4081 attgagatca ggtcgtctac aaatatggca gtttcttttg aattcaaata cttttttgtt
     4141 ccctccagaa gcagcctgaa atgtttcaga caaaatccat tttgccggtc aaacatggct
     4201 ttgaaatctt cttccttgaa gtacaaatag agtataacat ctatgtacct gtccattgta
     4261 tagctgattc tgctgcaaat ggtgcaggaa ttatcgagat tatccagctt gtttattaat
     4321 tcgtctacaa acttttgtgt ttctgtttgt tttgaggaaa gcttgttggt gatattcttc
     4381 attaaagatg cctcggcgtc tttcttgagc aggttttttt tgctttcata cagcttattg
     4441 agctcagaat tctgttcctg catatgggtg tcgacgataa gccccaaccc cagcctgttt
     4501 acctgtgagt tgtacaaaag ctcgaaatgc cttctgcaaa aaccgctctc gtttgtctgt
     4561 atccttcctt ccggttccat tagaaagggc ccgagtatgt attctatgtt ttcgtcctcc
     4621 agcttttttt caagcacgca catgggacat tcgcattcgg ttttaaaagc atccgttacg
     4681 gggattgtgt atattttttc tttcataagc gtttcctttc gaagtttaat ataatatata
     4741 agaggtatgc ttaaattata gcaaaaaaac agggtattat atataataaa ttaaatatcg
     4801 gactgtttgt ttatgctttt atcaatcaat caatcatatt attatttaca gctttttact
     4861 ttgcgattta tacacagtaa acagtccgaa aaagacggga cgggtgatta aatatatgga
     4921 atacaaagga tataaaatta tggaggaaag aaacagggta attgttgaaa atgtcaatga
     4981 ctttaatccg ttgcatatat ttgagtgcgg gcagtgtttc agatggctga ggcagcctga
     5041 cggaagctat acaggggtgg caaagggcag ggttataaat gtcagctata atgatggaag
     5101 acttgagctt tacaataccg gtattgagga gtttaaagcc ctatggtttg actactttga
     5161 tctgggaagg gactattcgc ttgtcaaaga gaaggtgatg aaggacgaag tgatgaagaa
     5221 ggcagtttct tttggctcgg gaataaggat tcttaagcag gatatttggg agactctcat
     5281 atccttcata acttccgcaa acaacaggat accgatgata atgaagactg taaatgcgat
     5341 ttctgaagca tatggcgagg aaattgaaat ggacaaaaag aagtattatg ctttcccaaa
     5401 atgcagtgag atttcccggt ctactgccgg gaaattggag ctttgcgggg gaggcttcag
     5461 atgcaagtac atactggcca cttccaagat gattgatgaa ggcgaaatga gtcttgatgc
     5521 cctggctttc atggagacaa aggatgcccg ggagtacctc aagaggtttc cgggagttgg
     5581 tccgaaggtt gcggactgta ccttgctgta cagtggtaca aaatatgatg tatttcccac
     5641 ggatgtttgg gtgaaaagag taatggagga gctatacttt aaaagagaaa cgggatttgc
     5701 cgaaatacaa aactttgccc gggactactt cggagagtat gccggatttg cccagcagta
     5761 tctgttttat tatgcccggg aaaacaaaat cggagcaaaa taatcgataa tcctgttgaa
     5821 caatcttgtg atatatttta acataattaa ctataatagt tttttgtgta tgcaatttgt
     5881 aaaattaaat gtggtcttga tttattaagc atttaaaagt ttaataataa tatactcaag
     5941 ttaatagctt taattgaata ctattttgtt tgtttttata aggcataggg tgatataatg
     6001 tttttgaggt tagtgaaagt tgttaagata agcctgaaat gttcggataa acctattatt
     6061 ttgaacctac atttgacata agggagttta gagtttaaag gcaagatgtc aggcctcaag
     6121 ttaattccgt tacaaatctc gggtttgtac aacggcggtg gaagacagaa gcttttaaca
     6181 ggacacccac ctagctaggg ctaggtgtca aaatataggt gaacggcatt ttgggtattt
     6241 aatataagaa aaggccccgg catttatatg ctgaggtctt tccttatatt aatcggtagt
     6301 acagggtaaa gagaattatt acattattgt tcttccggga atgacagcat caattatacc
     6361 gtaaatcaat gcacctacca gggcaccgat caatgttaca ct
//
LOCUS       DAOOJC010000090         6390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_10002_length_6390_cov_3.906393, whole genome shotgun sequence.
ACCESSION   DAOOJC010000090 DAOOJC010000000
VERSION     DAOOJC010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10002_length_6390_cov_3.906393"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1749
                     /gene="nadB"
                     /locus_tag="PLA01_03780"
     CDS             151..1749
                     /gene="nadB"
                     /locus_tag="PLA01_03780"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPD00452.1"
                     /translation="MDDYRYLVDFDTKDLIIEEHDIVIIGSGIAGVYTALEIPEKYDI
                     AILTKESIEISNSVLAQGGIAVSLDKGDSPELHFKDTIYAGAGLCNEETVWVLVKEAA
                     ENIDALCKFGVEFDRKSKNELCLSREGAHSKNRIIHAGDTTGKEVCDKLISIVRTRNN
                     VEIKERTFALDLITQKDVCKGVLAYDEDKDTYILYMANVVVCATGGFGQLYSNTTNPE
                     VATGDGVCLAYRAGAELMDLEFIQFHPTVLFHPENKSFLISEAVRGEGALLKNIHGER
                     FMPKYHELNELAPRDIVSRSIFSEMKKTNSDHVYLDITFKGKDYLEHRFPNIYKTCMS
                     YGIDMSKDFIPVAPAEHYCMGGIRTDVWGSTNIKGFFACGETACNGIHGANRLASNSL
                     LEGLVFGHKIGNKIEDILSSSKKGQESINIKSETARLVKDIDTEKIKQDIKRTMTKYV
                     GIARDKEGLEKAKQKVDSYYEMISDMKNTNIHHYEIQNVVLLSKLVIESALERKESRG
                     AHFRLDFQKTDDINWKRNIIKRRI"
     gene            1778..2611
                     /gene="nadC"
                     /locus_tag="PLA01_03785"
     CDS             1778..2611
                     /gene="nadC"
                     /locus_tag="PLA01_03785"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPD00453.1"
                     /translation="MKYITDMDRIIMNALKEDIPSGDITTDNIIDEASESEAVLISKA
                     EGVIAGLDVAKRVFMLLDDKIVFEKKVDEGQRVKYGDTIAVIKGNTRAILKAERTALN
                     LLQRLSGIATATRKLVDRIEGLHAKVVDTRKTTPGLRILEKYAVRVGGGYNHRFSLSD
                     GVLIKDNHIKAAGGIKNAIERVREKIPHTLKIEVETETIEQVLEAIDGKADIIMFDNM
                     GLDKMREAVKIINGKIITEASGNVTLDTIYDIASTGVDIISVGGLTHSVHSFDISMKI
                     S"
     gene            complement(2890..3993)
                     /locus_tag="PLA01_03790"
     CDS             complement(2890..3993)
                     /locus_tag="PLA01_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPD00454.1"
                     /translation="MIALILFISFVHFENIYPFLYHVFYDKDDLNIYISFINTQVRSV
                     SSEPSNNVYLKAVVKDSSGKPIPNISIDFLNDSGMGNIKPQASHTNHMGECIATYIPP
                     YYYDIGNGKASVDITAAIAGTNKAGQLQIDLFPIPVVFVHGYLEGPGVFDNLNEFLTS
                     KGFCCSSISYDSTRGVEDAAKELNIFLQQQKKDFMDKGILVNRFDLITHSMGGLVARY
                     YSGSTEYIKNDDINKIIFLSVPHKGSILASIAEEYFNDRSIKNLMPDDELFTNTFAGM
                     INGGLNNSVQVGNLLSQYDEVVSEENASLEEWGIKTEIFGVGDNSFTVDNLLSGNILH
                     APNHKGILNNKRVFDRVYEMLNSNLSCPSAVKK"
     gene            4282..4713
                     /locus_tag="PLA01_03795"
     CDS             4282..4713
                     /locus_tag="PLA01_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPD00455.1"
                     /translation="MKVKDKMTKSVAYVDPATNMVEVAQLMQKHNVGSIPVYDQGSVV
                     GIVTDRDIVVRNVAHGKSPKDTKVQDVMTSQVTTVSPDMEIDEVTKIMAQQQIRRVPV
                     VENNQIVGMLALGDIASDVRFDMEASEALSEISRPSKPFRI"
     gene            complement(4965..5348)
                     /locus_tag="PLA01_03800"
     CDS             complement(4965..5348)
                     /locus_tag="PLA01_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00456.1"
                     /translation="MQTAYVQSGTPVSPPAIIPPVPTGYPSGPSYVSTPPQIQQASQP
                     APDSDTMGPAPETVESIAYTQGYLKQHIGERVKVEFLIGTNMLIDREGTLVDVGASYI
                     VIQEAETDDLLLADMYSIKFVRFYY"
     gene            complement(5456..5899)
                     /locus_tag="PLA01_03805"
     CDS             complement(5456..5899)
                     /locus_tag="PLA01_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HPD00457.1"
                     /translation="MPMSTRELFARLIKCEAGGEGLDGMRAVATVVMNHVHVAYGEYL
                     RTGQGGLRKVIFQPRQFTCVMEKVYGEVNPQTIWATPPEQIHYDVADWALSGNKLPGI
                     GECLWYYNPFSANCSYLFPATGTGKFITRVNQHCFYVPTDLYAQT"
     gene            6155..6328
                     /locus_tag="PLA01_03810"
     CDS             6155..6328
                     /locus_tag="PLA01_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HPD00458.1"
                     /translation="MSRNRGMMSERLKEEIAKELGVYNTVASEGWGSVTSRDCGNIVK
                     KAIEMAERNLTNR"
BASE COUNT         2074 a         1117 c         1392 g         1807 t
ORIGIN      
        1 attaaccttg atgaagacat cagattaaag gcgaagaggg ctctggaccg tatgctgcaa
       61 atcggctagt acttttgagc tttcataaaa cacaatttca cgaaattatg ttgccataca
      121 tataatctat attagggagg aagtatccgt ttggatgatt atagatattt agtggatttt
      181 gatacaaagg atttaataat agaagaacat gatattgtca taataggcag tggcatagca
      241 ggagtctaca ctgcccttga aatacccgag aaatatgata ttgccatatt gacaaaggaa
      301 tctatagaga taagcaattc ggttcttgcc cagggaggca tagccgtttc tcttgacaag
      361 ggagattctc cggaactgca ttttaaggat acgatttatg ccggtgccgg tctttgtaat
      421 gaggaaaccg tatgggttct tgtaaaagag gccgcagaaa acatagatgc attatgcaag
      481 tttggagttg aatttgacag aaagagcaag aatgagcttt gcctttcaag agaaggggcc
      541 cacagcaaaa acaggataat acatgccggg gatacaacgg gtaaagaggt atgtgacaag
      601 ctgatatcga tagtaaggac gagaaataat gtagaaataa aggaaagaac ctttgcgtta
      661 gatcttataa cccaaaagga tgtctgcaag ggtgttttgg cttatgatga ggataaggac
      721 acttatattt tgtatatggc taatgtagta gtttgtgcca ccgggggttt cggacaattg
      781 tattcaaaca caacaaatcc cgaggtggcg accggagacg gagtttgcct tgcatacagg
      841 gccggggccg aattaatgga tttggagttt attcagtttc atccgacagt gctcttccat
      901 ccggaaaaca agagttttct tatttctgaa gccgtcaggg gagagggggc actattaaag
      961 aacatacatg gcgagaggtt tatgcctaaa taccatgaac ttaatgaact ggccccaagg
     1021 gatattgttt caaggtctat cttcagcgaa atgaagaaga ctaactccga tcatgtttat
     1081 cttgatataa cgtttaaagg aaaggattac ctggagcata gatttcccaa catttataaa
     1141 acctgcatga gctatggaat agatatgtca aaggatttta ttcccgtagc tccagcagaa
     1201 cactattgca tgggcggaat caggacggat gtttggggaa gtaccaatat aaagggcttt
     1261 tttgcatgcg gagaaactgc ttgcaatgga attcacggtg caaaccggct ggccagcaat
     1321 tctcttcttg aaggcttggt tttcgggcat aaaatcggta ataaaatcga agacattcta
     1381 agcagcagta aaaaaggaca ggagagtata aatataaagt cggaaaccgc caggcttgtc
     1441 aaggacatag acacagaaaa gataaagcag gacattaaaa gaaccatgac caaatatgtc
     1501 gggatagcca gagacaaaga agggcttgaa aaggcaaagc agaaggttga cagctattat
     1561 gagatgataa gtgacatgaa aaacaccaat atacatcact atgagattca aaacgttgta
     1621 cttctgtcaa agcttgtaat tgaatcggct cttgagcgta aagaaagccg tggtgcccat
     1681 ttcagactgg attttcaaaa aaccgacgat ataaactgga aaagaaatat aataaaaaga
     1741 agaatttagt acatactaat aaggatggtg cgagtaaatg aaatatataa cggatatgga
     1801 tagaataatt atgaatgcgt taaaggagga tattccttcc ggggacatta caacggataa
     1861 tattatagat gaggcatcgg aatcggaagc agtgctgatt tccaaagccg aaggcgtgat
     1921 agccgggctg gatgtagcaa aaagggtctt tatgctcctt gacgataaaa ttgtgtttga
     1981 aaagaaggtt gatgaaggtc aacgggtgaa atacggtgat accattgcgg taataaaggg
     2041 taatacaagg gcgatattaa aggcagagag gacagctctg aaccttcttc agaggctttc
     2101 gggaattgca actgccacaa gaaagcttgt ggacagaatt gaaggattgc atgctaaggt
     2161 ggtggatacc agaaagacaa ctcccggact caggatattg gaaaagtatg cagtgagggt
     2221 cggtggcgga tataatcaca ggtttagcct ctccgacggg gtattgataa aggataatca
     2281 tataaaggca gccggaggaa taaaaaatgc aattgaacgt gtgagagaaa aaatacccca
     2341 cacactgaag atagaagttg aaaccgagac tatagaacag gtgttggagg caatagatgg
     2401 aaaagcagat ataataatgt ttgataatat gggacttgac aaaatgagag aagctgttaa
     2461 aataataaat ggtaagatta taacggaggc atccggaaat gtaactttag atacaatata
     2521 cgatatagct agcacgggag tggatattat ttctgtcgga ggccttacac attcggtaca
     2581 ttcgttcgat attagtatga aaatcagtta gtgtagtttt attaagttta gtcaagttca
     2641 aaaataaaat tcttttttat gagcagtttt tcattattta agtaaagatg taatgacagc
     2701 tgtggataaa ccagcaaaat atccacagtt aattcacatt atttttgttg tcataattct
     2761 aagatgatag aaactgcatg aaacaagtgt ttgaggagta agtgtataag aaaagatatc
     2821 cacaagcctg tggatatagt acttttttta tatgcttttt ttatttatac cggttgttaa
     2881 atataaccat catttcttca cagcagaagg gcaggacaaa ttggaattga gcatttcgta
     2941 aacacggtca aagacccgtt tgttgtttaa tatgccctta tggttcggag catgaagaat
     3001 attaccgctc aacaaattat ccactgtaaa gctgttatcc ccaactccaa aaatttccgt
     3061 tttaatgccc cattcctcca ggcttgcgtt ttcttccgaa accacttcat catattggct
     3121 caataaattt ccgacttgaa ccgagttatt cagtccgccg tttatcatgc ctgcaaatgt
     3181 atttgtaaac aattcgtcat cgggcatcag attctttatt gaccggtcat taaaatattc
     3241 ttcggcaatg gatgcaagta tggaaccctt atgggggaca gaaaggaata tgattttgtt
     3301 aatatcgtca tttttaatat attccgtgct ccccgaatag tacctggcca ccaatccgcc
     3361 catgctgtga gtaattaagt caaacctgtt aaccagtatt cctttgtcca tgaaatcctt
     3421 tttttgctgc tgcaaaaaaa tattaagctc ctttgccgca tcctcgactc cccgggtgga
     3481 atcataactt atcgaactgc aacaaaatcc cttggatgta aggaattcat ttaaattgtc
     3541 aaatacaccg gggccttcta gataaccgtg aacaaaaacc acaggtatcg ggaaaagatc
     3601 aatttgcagc tggccggctt tatttgttcc tgctatagca gcggttatat cgactgatgc
     3661 tttaccgtta ccgatgtcat aataataagg aggaatatat gttgcgatgc attcgcccat
     3721 atgatttgta tgtgaagctt gaggctttat atttcccata ccgctgtcat ttaaaaaatc
     3781 tattgatata ttgggaatgg gtttacctga cgaatccttt accactgcct tcaaatacac
     3841 gttgttggaa ggctctgatg aaacggaacg aacctgtgtg tttataaaac tgatatatat
     3901 gtttaagtca tccttatcat aaaagacgtg gtacaaaaaa ggatatatat tttcaaaatg
     3961 aacaaaggaa ataaaaagta ttaacgctat cattatcaaa attatttttt tatgtattga
     4021 atgcataaaa tcaatccttc aagatatgat tatttttaat tatagcattt taagtgcata
     4081 ttataaacag caaacagaga gtttatatta gtaaatagtg gaagccgtat aaaaggttct
     4141 gcaactccct tctcaatgat actgtcatat tttccttcaa ttaaaaaatg catccagata
     4201 catcagtagc acaaatttca ttaattaaat actcttttaa ggagaaaata aaactgaaaa
     4261 aataattagg agatgatcaa tttgaaggtt aaagataaaa tgacaaaaag tgttgcatat
     4321 gtagaccctg ctacaaatat ggttgaagta gctcagctga tgcaaaaaca caacgtaggt
     4381 tccattccgg tgtatgatca gggttcggtt gtcggaattg tgaccgacag ggatattgta
     4441 gtaagaaatg tggcccacgg taaatctcca aaggacacta aagttcaaga tgtaatgaca
     4501 tcacaggtta caacggtttc tcccgatatg gaaattgacg aggttactaa gataatggca
     4561 caacaacaga taagaagggt tcctgtagtg gaaaacaacc agattgtcgg catgcttgca
     4621 ttaggggaca tagcatccga tgtgaggttt gatatggaag catcggaggc attaagtgag
     4681 atatccagac cgtcgaagcc ttttagaatc taatgcattg atgaggatag atataacaag
     4741 gaaagagttg aatgtcaaaa tcaactcttt ccttgtactc ctttcatggc ttcattaaaa
     4801 ggttctgcac ctttctatgc aatgtttcat ccatgttttg cttcacttcg gcaaaagctc
     4861 caggggcatc gagcccctat cgctttttgg tcatttcact tcgtgaaatc ccactaacaa
     4921 aagcatccta ttaatgatgc tttttattat ggaatatgaa ggtattaata atagaacctc
     4981 acgaatttga tagaatacat atctgccagt aaaaggtcat cggtttctgc ttcctggatt
     5041 acaatataac ttgcacctac gtctaccaag gtaccctctc tgtctataag catatttgtt
     5101 ccaatgagaa attccacttt aaccctctcg ccgatatgct gtttcagata tccctgggta
     5161 taggctatac tctctaccgt ttcgggagca gggcccattg tgtccgagtc aggtgccggt
     5221 tgactggcct gttgtatttg tggaggcgtt gaaacatatg aaggtcctga agggtagccc
     5281 gtaggtaccg gggggatgat tgccggcggt gaaactggtg taccgctttg cacatacgcg
     5341 gtttgcatgg gctgcatata ttggtatgca ggtgaatact ggttctggtt catataatta
     5401 ttcataatat cctccttaaa tgttgaattt attgcttatt tcacggcaca aagttttatg
     5461 tttgggcata aagatctgtt gggacataaa aacagtgctg attaaccctt gttataaact
     5521 tccctgtacc ggttgcgggg aataagtaac tgcagttggc actgaacgga ttataatacc
     5581 agaggcactc tcctattccc gggagtttat ttcccgaaag tgcccaatca gcaacgtcat
     5641 aatgaatctg ctccggtgga gttgcccata ttgtctgggg atttacttcg ccataaacct
     5701 tttccataac gcaggtgaac tgcctgggtt ggaatataac tttacgaagg cctccctggc
     5761 cagttcttaa atattctccg taagctacgt gcacgtggtt cattaccaca gttgcaaccg
     5821 ctctcattcc gtcaagccct tctcctcccg cttcgcattt tataagccgt gcgaacagct
     5881 ctcttgtaga cataggcata gttatcaccc taacttttaa tgatagtaaa cagtaataaa
     5941 attatgatgg attcgattcg aaggttaatt tttaacaagt acattgtgtt ggcctaatac
     6001 agtatattag caaagggcac ggttggtaac cgcattttta taatcgactg atgaaaaaat
     6061 tgcatcaaat tattatttaa aatttgaaga taataatatt gaacgcatta tttttggagc
     6121 aaaaacaatt tgctgcaaaa aggaggcaaa tacaatgagc aggaatagag gaatgatgtc
     6181 agaaaggctt aaggaagaaa tcgcaaagga acttggggta tacaacacag ttgcaagtga
     6241 aggctggggc tcggtaacct caagggactg cggtaatatc gtcaagaaag cgatagaaat
     6301 ggcagaaaga aacctcacaa accgatagta ttgccttaaa tagagtagag aaataattgc
     6361 taagaattat ttccctctcg ttgaaccgta
//
LOCUS       DAOOJC010000091         6363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_10040_length_6363_cov_3.328472, whole genome shotgun sequence.
ACCESSION   DAOOJC010000091 DAOOJC010000000
VERSION     DAOOJC010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10040_length_6363_cov_3.328472"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..955
                     /gene="speB"
                     /locus_tag="PLA01_03815"
     CDS             86..955
                     /gene="speB"
                     /locus_tag="PLA01_03815"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HPD00459.1"
                     /translation="MSLKDGNMTLPTKFMASTEYFGEADIVLVGVPMDFTCSFRPGTR
                     FGPQKIREVSIGIEEYSIYMDRDLTEFSFYDAGDLDLPIGNVNKSLEIIESAAEEVIK
                     DNKFPFFIGGEHLISVPVIKKVYEKYGDDLVVIHFDAHADLREGYLGCPNSHASAIRR
                     VIDFMPGKNIYQFGIRSGTKDEFEFARKNTNMFTFDVIEPLKQCIDKFKNRPLYVTLD
                     IDVLDPAYANGTGTPEPGGISSKELLQSIHLFKDHNIVGFDLVEVSPPYDPSDRTALI
                     AAKIIRDVIMMVG"
     gene            959..1522
                     /locus_tag="PLA01_03820"
     CDS             959..1522
                     /locus_tag="PLA01_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD00460.1"
                     /translation="MQTIKNSLGQSHDYIRLFVIEGDTVVDATCGNGNDTAFLAGLVG
                     EKGKVYSFDIQDRALCNAKKRIDELGLTKRVELIKDGHENMDKYVNMPVKAVMFNLGY
                     LPGGDHKIGTKPQTTIQAIKKAMNLLIKGGVIVIVIYYGGDSGFEEKERVLEFLTGID
                     QKEYIVMKNEFINQANCPPILVCIEKL"
     gene            complement(1526..2260)
                     /locus_tag="PLA01_03825"
     CDS             complement(1526..2260)
                     /locus_tag="PLA01_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPD00461.1"
                     /translation="MSKNTPTQSDSNIFCKVSINNDGNWIYGIITYLNLDKNIAEIFL
                     PARFFKSYLKEGSKVLVKAISDNNETLFSGSITRKVISIRKQAVTVQLDSIMSFENKR
                     NYERFYVNYPCKIREENSIAYMATLADISFKGGLVYTENLLEEGKNVIVDIYLNPSMT
                     LTFSGKIARKLKDKNRGFCYGIELTKIDEENSILLNEVIECLVNKREQIAYEWKVFKR
                     LKYAIYAISVLGIFLTVFSIFITEVL"
     gene            2635..4185
                     /locus_tag="PLA01_03830"
     CDS             2635..4185
                     /locus_tag="PLA01_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPD00462.1"
                     /translation="MDRADRIRLLHEKKAQLEQGGGAEKIAKQHESGKMTARERIEAL
                     FDENSFVEIDAFVETKSIDFDMQKKKVAGDGVVTGYGSVNGRLVFVSSEDFTVIGGSL
                     GEMHAKKITKVMDMAMKMGVPFININDSGGARIEEGIDALKGFGDIFFRNTLASGVIP
                     QISVIMGPCAGGAVYSPAITDFVFMVEKTSQMFITGPQVIKAVTGEDVTFEELGGADA
                     HNSISGVAHFKSSNEKECIEKIKRLIDFLPDNSLSDVPVYDTGDNLNRINENLLEIMP
                     DDSNKPYDMMEIITSVVDNGDFLEIQEHFAKNIIIGFGRMNGGTVGIIANQPKYSAGV
                     LDVNSSDKAARFVRFCDSFNIPVITFTDVPGYLPGVGQEHNGIIRHGAKLLYAFSEAT
                     VPKINVIVRKAYGGAYIAMNSKHLGADMVFAWPSAEIAVMGPDGAANIIFRKEIKEAN
                     DPVATRTERIEEYRNKFSNPYIAASRGYVDDVIDPASTRIRLISALEMLASKRDNRPS
                     KKHGNIPL"
     gene            4215..4385
                     /locus_tag="PLA01_03835"
     CDS             4215..4385
                     /locus_tag="PLA01_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium pump decarboxylase subunit gamma"
                     /protein_id="HPD00463.1"
                     /translation="MKDQLNEEIILAISAALAAYETRPDYKLVVKSFKRISQTSPVWS
                     TTGRLEGIGRSI"
     gene            4412..4816
                     /locus_tag="PLA01_03840"
     CDS             4412..4816
                     /locus_tag="PLA01_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPD00464.1"
                     /translation="MKKFLIKVNGSQYEVEVEEIRNGVASEPQVTLSQSAPKAAPAPT
                     PAPAPAAAPAPKADSSVPAGANVIKAPMPGTILKVNVNTGDAVKKGQVLLILEAMKME
                     NEILAPNDGTVASVNVSQGSSVNVGDVMISLK"
     gene            4876..6273
                     /locus_tag="PLA01_03845"
     CDS             4876..6273
                     /locus_tag="PLA01_03845"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HPD00465.1"
                     /translation="MAKVRITETVLRDAHQSLIATRMRIEEMLPVIEKLDDIGYNSLE
                     VWGGATFDACLRFLNEDPWERLRTIKKHCKKTKLQMLLRGQNLLGYKHYADDVVEYFV
                     QKAVANGMDIIRIFDALNDPRNIETAIKACKKEGGHAQAAVSYTISPVHNLDLFVKDA
                     KRLVDMGADSICIKDMAGLLVPYAAYDLVKALKQNIKVPIQLHTHYTSGVASMTYLKA
                     IEAGCDVVDCAISPMAMGTSQPPTEPLVASLKDTPYDTGLDLDKLSEIAEYFRPLKEE
                     YISKGLLDVKVMGVDVNTLKYQVPGGMLSNLVSQLKQSNALDKYEEVLKEVPRVREDF
                     GYPPLVTPSSQIVGTQAVLNVVTGERYKMVPKESKALVKGEYGKTPAPINSEVQKKIL
                     NGEEPITARPADFIKPELSNIREEMKEYLEQDEDILSYAMFPQVAKKFFEFRKAKKYK
                     IDPDMVDYENKVHPV"
BASE COUNT         2068 a         1134 c         1390 g         1771 t
ORIGIN      
        1 tacgtgcggt tcaacgagag ggaaataatt cttagcaatt atttctctac tctatgcaaa
       61 atatttatta aaggagtatg gcagaatgag cttgaaggat ggaaatatga cacttcctac
      121 aaagtttatg gcaagcaccg aatactttgg agaggccgat atagtgttgg taggcgttcc
      181 catggacttt acatgcagct tccggcccgg tacaaggttt ggacctcaaa aaatcaggga
      241 agtgtccata gggattgaag aatacagcat atacatggac agggatttga cggagttctc
      301 cttttatgat gcaggggatc tggatcttcc catcggtaac gtgaataaaa gccttgaaat
      361 aattgaaagt gccgcagaag aagtaatcaa ggataataaa tttccgttct ttattggagg
      421 cgagcatctt attagtgtac cggttataaa aaaggtatac gaaaaatacg gtgatgactt
      481 ggtggttatt cacttcgatg cccatgccga ccttcgggag gggtatcttg gttgtcccaa
      541 ttcccacgct tctgccataa gacgagttat tgatttcatg cccggcaaaa acatatatca
      601 gtttggcata aggtcaggca ccaaggatga atttgagttt gcccgcaaaa acaccaatat
      661 gtttactttt gatgtaatcg agccgttaaa acagtgtatt gacaagttta aaaaccggcc
      721 attgtatgta actttggata tagatgttct ggatcccgcg tatgccaacg gtacaggtac
      781 accggagccc ggaggaatca gttcaaagga attgcttcag tcaatacacc tttttaagga
      841 ccataatatt gtcggatttg acctggtgga agtttctccg ccgtacgatc cttcggacag
      901 aacagcttta attgctgcta aaataataag agacgttatt atgatggtgg gctgaattat
      961 gcagacaata aagaattctc tcggacaatc ccatgattat atcaggctgt ttgtaataga
     1021 gggcgatacg gtagtggacg ccacatgcgg taacggaaac gataccgcat ttctagccgg
     1081 tctggtgggg gaaaagggca aggtttacag tttcgatata caggataggg cattatgtaa
     1141 tgctaaaaaa agaatagacg aacttggtct tacaaaaagg gtcgaactga taaaagacgg
     1201 gcatgagaac atggataaat acgtaaatat gcccgttaaa gccgtgatgt tcaatctggg
     1261 ttacctgccc ggaggtgacc ataaaatcgg aacaaagccc cagacaacca tacaggcaat
     1321 aaagaaggct atgaatctcc ttattaaagg gggagtaatt gttattgtca tttattacgg
     1381 aggcgacagc ggctttgaag aaaaggaaag agttctggag tttttaaccg gcatagacca
     1441 aaaagagtat attgtaatga agaatgaatt cataaaccaa gcaaactgtc ctcccatatt
     1501 ggtttgtatt gaaaagcttt agtattcaca gtacttcagt tatgaaaatg gaaaatacag
     1561 tcaagaaaat gcccagtacg gatatagcat aaatagcata tttgagcctt ttaaacacct
     1621 tccattcgta ggcgatttgc tcccttttat taacaagaca ctcaattact tcgtttagca
     1681 gaatactgtt ttcttcatct attttggtaa gctcaattcc atagcagaat cccctgtttt
     1741 tgtccttaag ttttctggct attttgccgg aaaaggtcag agtcatgctt ggatttaaat
     1801 aaatgtccac gataacgttc ttgccttctt ccagcaggtt ttccgtataa accagtccac
     1861 ccttaaagct tatatctgca agtgttgcca tataggcaat actgttttct tcccggatct
     1921 tgcatgggta gttgacataa aatctctcgt aatttctttt attttcaaag ctcataatac
     1981 tgtcgagctg tacggtaact gcctgttttc ttattgaaat aacttttctt gtaatgcttc
     2041 cagaaaacag tgtttcgttg ttatcgctta ttgctttaac caatactttg cttccttctt
     2101 ttaaataact tttaaaaaat ctcgcaggca aaaatatctc agcaatattc ttatcaagat
     2161 tgagataggt tataattcca taaatccagt tcccgtcatt attaatcgaa actttgcaaa
     2221 aaatattcga atcgctttgc gtaggtgtat tttttgacat aaaaccaccc tgcctttaaa
     2281 aatatttatc tatttttatt tatcggataa aaaaagaaat ttctagatag caaattggca
     2341 tgtttctgcc tgaaaagata aaaaagattt gatgaaaata ttattatttt gtaaatttat
     2401 ttctttctta aatatcagtt atatgatata aaagaattgg gaaaattatt gcaatatacg
     2461 aaaaagtaag atataattta ttttaattat aacctggtaa taatatcata taataatgct
     2521 tgaaataatc agaaatcaag ttaataatta ggattgacag ataaatacag catactcaat
     2581 tgaacagcgg aactactttt tacaaacaat ttaaaaataa gaggtggaaa attgatggac
     2641 agagcagaca gaataagact tctccatgag aaaaaagccc aattggaaca gggtggggga
     2701 gcagaaaaaa ttgcaaaaca gcatgaatcc ggcaaaatga ctgcgaggga aagaatagaa
     2761 gctttatttg acgaaaatag ctttgttgag attgatgcgt ttgtcgaaac aaaaagcatt
     2821 gactttgaca tgcaaaagaa aaaggtcgct ggagacggag ttgtaacagg atacggttct
     2881 gttaacgggc gactggtgtt tgtttcatcc gaggatttta ctgtaatagg aggttccctg
     2941 ggagaaatgc atgccaaaaa gataaccaaa gtaatggaca tggcaatgaa aatgggtgta
     3001 ccttttatta acatcaatga ctccggtggt gcaagaattg aggaaggaat tgatgcacta
     3061 aagggttttg gagatatttt cttcaggaac actcttgcat caggtgtaat accacagatt
     3121 tcggtgatta tggggccatg tgccggcgga gcggtatatt ctccggcaat aaccgacttt
     3181 gttttcatgg tggaaaaaac cagtcagatg tttattaccg gtccccaggt aataaaggct
     3241 gttactggtg aagatgttac ttttgaagag cttggcggtg cagatgccca taattccata
     3301 agcggtgttg ctcatttcaa aagttcaaat gaaaaggaat gtattgaaaa aatcaagaga
     3361 ctcattgatt tccttcccga taacagtctt tccgacgttc ctgtttatga taccggagac
     3421 aacttgaacc gaatcaatga aaaccttttg gaaatcatgc ccgatgactc gaataagccc
     3481 tatgacatga tggaaattat tacgtccgtt gtagacaacg gtgatttcct tgaaatacag
     3541 gagcattttg ctaaaaacat cataataggc tttggcagaa tgaacggggg cacagtgggc
     3601 ataatagcaa atcaacctaa gtattccgca ggcgttctgg atgttaattc atcggataaa
     3661 gcagccaggt ttgtacgttt ctgcgattcc tttaatattc cggtaataac cttcactgac
     3721 gtaccggggt atttgcccgg tgtaggtcag gaacacaacg gaattatccg tcatggagca
     3781 aagctccttt atgctttctc ggaagctact gttcccaaaa ttaatgtcat tgtaagaaag
     3841 gcatatggag gggcatatat tgccatgaac agcaaacatc tcggagctga catggtattt
     3901 gcatggcctt cagctgaaat tgcggttatg ggtccggatg gtgcggccaa tataattttc
     3961 agaaaagaaa ttaaagaggc caatgatcct gtagctacaa gaactgagag aatcgaagaa
     4021 tacaggaata aattctccaa tccttatata gcggcttcaa gaggctatgt ggatgatgta
     4081 atagacccgg cttcaaccag aataagactg ataagtgccc ttgaaatgct tgcaagcaag
     4141 agggataaca ggccgtctaa aaagcatggg aatattcctt tataatatat attgtctctc
     4201 ttaaggagga tgctatgaaa gatcaattaa atgaagaaat aatcctggca atatcggctg
     4261 cattagcagc gtatgaaaca aggccggatt acaagcttgt agtaaaatct ttcaaaagaa
     4321 tatctcaaac atctccggta tggtccacca ccggaagatt ggaaggaatt ggaagaagta
     4381 tataatttaa aattaataag gaggattaaa catgaaaaag tttcttataa aagtaaatgg
     4441 aagtcagtat gaggtagaag ttgaggaaat cagaaacgga gttgcttcgg aaccgcaggt
     4501 caccctaagc cagtctgctc caaaggctgc accggcaccg acaccagccc cggctccggc
     4561 agctgcaccg gcaccaaaag cggactcgtc agtacctgcc ggtgctaatg tgattaaagc
     4621 tcctatgcca ggtacaattt taaaagttaa cgttaatacc ggtgatgccg taaagaaggg
     4681 tcaggtactt ctgatactcg aagcaatgaa aatggaaaat gagattctcg cacccaatga
     4741 cggtaccgtt gcttcagtta acgtatccca aggttcatct gtgaatgttg gagatgtaat
     4801 gatttcattg aaataatgaa acgttttata tgcagatagt gttgtaatat gttattaaag
     4861 aggaggtaaa aatagatggc taaggtaaga ataactgaga cggtactaag ggatgctcat
     4921 caatctctta ttgccaccag aatgcgaata gaagaaatgt taccggtaat agaaaagctt
     4981 gacgatattg gctataactc tttggaagta tggggcggtg caacctttga tgcctgcctg
     5041 agattcttaa acgaagaccc ctgggaaaga cttagaacca taaaaaaaca ctgcaagaaa
     5101 actaagcttc aaatgctcct taggggacag aacttactgg gatacaagca ctatgccgat
     5161 gatgtcgtcg agtattttgt gcaaaaggca gttgccaatg gaatggatat aataagaata
     5221 tttgatgcct taaacgaccc cagaaacatt gaaacagcta tcaaggcgtg taagaaagaa
     5281 ggcggacatg cacaggcagc agtttcgtat accataagtc ctgtacacaa tctggatctt
     5341 ttcgttaaag atgcaaaacg tcttgttgat atgggtgccg attccatatg tattaaggat
     5401 atggcaggat tattagttcc ttatgcggca tatgaccttg ttaaagcttt aaaacaaaac
     5461 attaaagtac ctatacagct tcacacccac tataccagcg gtgttgcttc catgacatat
     5521 ttgaaagcaa tagaagcagg ttgtgatgtt gttgactgtg ctatttctcc aatggctatg
     5581 ggaacctcac agcctccaac ggaaccgttg gttgcatcat taaaagacac accctatgac
     5641 acaggtcttg atctggataa actgagtgaa atcgccgaat acttcagacc gttaaaagaa
     5701 gagtatattt caaagggact tttggatgta aaggttatgg gagttgatgt aaatactctc
     5761 aaatatcaag tacccggcgg aatgctttca aaccttgtat ctcagttaaa gcaatccaat
     5821 gctcttgaca agtatgaaga ggttctgaag gaagttccga gggtaagaga agatttcgga
     5881 tatcctcctt tggttacacc gtcaagccag attgtaggta ctcaagctgt gctaaatgtt
     5941 gtaactggtg aacgttataa aatggttcca aaagagtcca aggctttggt taaaggtgaa
     6001 tacggcaaga caccggcacc tataaattcg gaagttcaaa agaagatttt aaacggtgag
     6061 gagccaatta cggccagacc tgccgacttt attaagcctg aacttagtaa catcagggaa
     6121 gaaatgaagg aatacttgga gcaggatgaa gacattcttt cttatgcaat gttcccgcag
     6181 gttgccaaga agttcttcga attcagaaag gcaaaaaagt ataaaataga tccggatatg
     6241 gttgactacg aaaacaaggt acatccggta taaatgggga gggcaaacgc cctccttctt
     6301 atataatcta atgctctcga aaactcgtat tgctttcgag gtctctattt caggagcaag
     6361 cta
//
LOCUS       DAOOJC010000092         6353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_10063_length_6353_cov_4.403144, whole genome shotgun sequence.
ACCESSION   DAOOJC010000092 DAOOJC010000000
VERSION     DAOOJC010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10063_length_6353_cov_4.403144"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1772)
                     /locus_tag="PLA01_03850"
     CDS             complement(<1..1772)
                     /locus_tag="PLA01_03850"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HPD00466.1"
                     /translation="MFKCLYFQFTTKLNTDPFLSSTNITMILSSFAVLLIITSLIAFI
                     FNKYRFIALFAFNLLLTVLLMADTNFFRYYYNLLTVPVFFQMDIKLLSSVDQSIMSLF
                     LIKDIVYLLDLPFMLMGLVLLHKNAEKLYFKKRIIRSVSLLVVGMVTFLSVFRCTDIN
                     TLAYNSNYSAKSLGVFYSHYYNTKLFIEESLLEDDILTDEDKTAIIEFYKNKSSKFTD
                     NRLNGIAKGKNLIVVQMEAMQQFVINRKIGGKEITPNLNKLIKECLYFDNIYYQVSGG
                     NTSDAEFLSNTSMYPVKEGSVYHRFAENTYYSLASIMKEQGYDTYSLHGFDRYFWNRD
                     EMYKSLQFDTFYSEENFVLDDFAGWDGQALSDSSFFRQSLEKIDTAKPFYSFFVTLSS
                     HHPFNYFEDFAFDVGEFEGTFIGNYLKAANYLDKCIGEFIDNLKQRGLYDNSLLVLYG
                     DHSAVPKVESDGLMQLLGIEYNNTDWTKLQKVPVIIHYPQQEESEVISTIGGQIDILP
                     TIANLMDFDVPYAFGKDLINTDENYAILRNGSVVTKDYIYLSDLKEVYDYTSGKPMDI
                     DLYTEEIKTFIDELNISDTIIYKNA"
     gene            complement(2099..3052)
                     /locus_tag="PLA01_03855"
     CDS             complement(2099..3052)
                     /locus_tag="PLA01_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HPD00467.1"
                     /translation="MDIRIIIIAVNFIVGTVLIIYGVNLMSTGLEKANTHRMKWCLEA
                     LTNNLPLALVTGTILTALVQSSTAITVITVGLVNSGLLNLTQAVGIIYGANIGTTITA
                     QLMSFKLPHAGYIIIGFGLLTAFRSKKNSVKNIGWAVTGLGLMFTGLNILNSGVPYIK
                     ESRIAIELFKAYGQNPLIGLILGMITTMLVHSSSATVGLTIVLYNAGLISFESALGLT
                     FGDNIGTCITAQTASIGTNISARRTAWAHTLYNVIGVAIAFIFLVPFSHLVKEITLYL
                     GQDKTRLVANAHTVFNILSALVFLPVTKYYVKFIQWVITEK"
     gene            3236..3574
                     /locus_tag="PLA01_03860"
     CDS             3236..3574
                     /locus_tag="PLA01_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtrH family sporulation protein"
                     /protein_id="HPD00468.1"
                     /translation="MSAFTSNIIYNFLISFGVIVGASLLAGIGALINDHPPLRCMFEI
                     ASSIKIWAVAVALGGTFSTFEILEKGLFRGEIRSIVKQAVYVVVAVIGANMGYGFIRL
                     IQKCGESMIK"
     gene            3571..4047
                     /locus_tag="PLA01_03865"
     CDS             3571..4047
                     /locus_tag="PLA01_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00469.1"
                     /translation="MKKLAYRLFICFITGLLGGILIGCTVMSTLVSYRIDRYHEEIMF
                     LKTVIADNDVKLKKLEESFDNVNKKKFLVSDIEVHLIYADGEENSFDKMELTNYIKDK
                     YKDLLWKEVRGIDTDLAVGLIDNDVLVIEGKSYELRVSRVLISDVFKIWVTVKNNQ"
     gene            4234..4761
                     /locus_tag="PLA01_03870"
     CDS             4234..4761
                     /locus_tag="PLA01_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPD00470.1"
                     /translation="MRTKEHNRDCYFNDLESFETIVLKYRENSINFAKRYINDIQICE
                     DIAQEAFADVYVNRDKYNPKISFKTWLFSIIHNKCVDYIRKNKELIIDSIEDKSREGN
                     EFDNLETKILLEHEIGKLKSNYRAIIQMVEVEGMTIREAAKVLQISEVHARVLNHRAK
                     KKLRKELMKGGFVYE"
     gene            4754..5059
                     /locus_tag="PLA01_03875"
     CDS             4754..5059
                     /locus_tag="PLA01_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00471.1"
                     /translation="MNDREFLDGVYRKINILERERMEMELIRKRQKRLFYRQVKMCLI
                     PFLSVAVLVIFLLLFEISMLETAVISASILLFMVWLEACDFEMENTDVGVRLNEYNY"
     gene            5043..5906
                     /locus_tag="PLA01_03880"
     CDS             5043..5906
                     /locus_tag="PLA01_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016298159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD00472.1"
                     /translation="MSIIINNLCKKYGNKVVLDNVNLQIDKGMFGLLGPNGAGKTTLM
                     RILTTLIPPTSGTIEVNGVQIHRRKEIREITGYLPQEFSLYPSFSVYEALDYLAVLSR
                     ITDNKMRKDIVNELLEKVNLRQYKKVKVKTLSGGMKRRLGIAQALLANPKVLIVDEPT
                     AGLDPEERIRFRNMLGSLSSERIVILSTHIVEDIESTCEKLAVLKEGKIVYAGTVGEL
                     LKSVEGYVWSGLIEPDKLNFVQDNFTLISSVRETEKFRVKILASEKPFEGAENILPSI
                     EDGYMKIIKGV"
     gene            5909..>6353
                     /locus_tag="PLA01_03885"
     CDS             5909..>6353
                     /locus_tag="PLA01_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00473.1"
                     /translation="MLLKLSVIEIKHQFKRITFYVFVLLTALFVFTQIGEFEMWSKHP
                     DEQEDIYLLADQMIGDPKQPDEMATQKGVYELLQRAVESKKIMVQGLIINKEKRLSEE
                     QIKLIKEVMQKISPEGKEIWTEEDLHLSGEEFEKIRLILGYSDNDP"
BASE COUNT         2081 a         1012 c         1326 g         1934 t
ORIGIN      
        1 gcatttttat atatgatggt gtcagaaata ttgagttcat caatgaacgt cttgatttct
       61 tcagtataca aatctatatc cattggcttg ccgctggtgt aatcataaac ttctttcaaa
      121 tcactgaggt aaatataatc ttttgttacc acggatccgt ttctgagaat ggcataattc
      181 tcatcggtat ttataaggtc ttttccaaaa gcgtaaggca catcgaaatc cattaagttt
      241 gcaatagtcg gaaggatatc gatttgtccg cctatagtac tgattacctc ggattcttcc
      301 tgctggggat aatgaattat aacaggaact ttttgaagct ttgtccaatc agtattgttg
      361 tattcgatac ccagcagttg catgagccca tcggattcaa cctttggaac tgcagagtgg
      421 tcgccgtaga gaactaaaag gctgttatca tataaacccc tttgcttgag attgtctata
      481 aactctccga tgcatttgtc cagatagttt gctgccttca ggtaattacc tatgaatgtt
      541 ccctcaaatt cgcccacatc gaaagcaaag tcttcgaaat agttaaaggg atgatggctg
      601 gacaatgtga cgaaaaagct gtaaaatggc tttgctgtgt ctattttttc aagggattgc
      661 ctgaagaagg atgaatcact gagtgcctgc ccgtcccatc ctgcaaagtc atccagtaca
      721 aaattttcct cgctgtaaaa ggtgtcaaac tgtaaagatt tatacatctc atctctgttc
      781 cagaagtatc tgtcaaagcc gtgaagagaa taggtgtcat aaccttgctc tttcattata
      841 cttgccaatg aataataggt attttcagca aatctgtgat ataccgaacc ttcttttaca
      901 ggatacattg atgtattgga taagaattcg gcatccgagg tattacctcc tgaaacctga
      961 tagtaaatat tgtcaaaata aaggcattct tttataagct tattaaggtt gggggtaatt
     1021 tccttgcctc caattttccg gtttataaca aactgttgca tagcttccat ttgcacgaca
     1081 ataaggtttt tgcccttggc aattccgtta agcctgttgt ctgtgaattt tgaactcttg
     1141 tttttgtaga actctattat tgcagtctta tcttcgtctg ttaaaatatc gtcttccaat
     1201 aggctttctt caataaaaag ctttgtatta tagtagtggg aataaaatac tccgaggctt
     1261 ttggctgagt agttgctgtt gtaagcaagg gtgtttatgt ccgtacacct gaagaccgaa
     1321 aggaaggtaa ccattcctac taccaataag cttaccgaac ggattattct tttcttgaaa
     1381 tacagctttt cagcattttt atgtagtaat acaaggccca ttagcataaa aggcaaatct
     1441 aaaaggtata ctatgtcttt aattaagaac aggctcatta tactctggtc tacggaactg
     1501 agcagcttga tatccatctg gaaaaacacg ggtacagtga gcagattata ataatacctg
     1561 aaaaagtttg tatcagccat taacaaaaca gtcagcaata aattaaaggc gaaaagagca
     1621 ataaacctat atttattaaa aataaaagca attaaggatg taattattag cagtactgca
     1681 aaagatgaaa gtatcatggt tatgtttgtt gacgaaagaa acggatctgt gttcagtttt
     1741 gtagtaaact gaaaatacaa gcacttaaac aaaatacaaa aaatgaacaa aagccaataa
     1801 agcatttctg cataattaaa acgttgccta acaatatttg ataagttttt catttcaaaa
     1861 ctaacctcgc tttaatgtga ttgttacgga ctatattatt tccaacttca taaatagttc
     1921 attttttact aattcgacca ttaatttaac ttctttatta tacatgattt tattgtaata
     1981 tgaaatacta aatttttaat aaacaaaatg ttaacaagtc ataaattttt gagaaatatt
     2041 tgaattcaca atatagtacc ggtgaaatta atatatgaat accggtcgta agagtttatt
     2101 atttctccgt tattacccac tgaatgaatt taacgtagta ttttgttacg ggaaggaaaa
     2161 caagggcact tagaatatta aatacagtat gggcattggc aacaagcctg gttttgtcct
     2221 gtcccaaata taaggtaatt tccttgacca ggtgggaaaa aggtacaagg aaaatgaaag
     2281 caatggctac cccaataaca ttatagaggg tatgtgccca tgcggttctc cttgccgaga
     2341 tgttggtgcc tatactggca gtctgtgccg tgatgcaagt tccgatgttg tcgccgaagg
     2401 tcaggcccaa ggcggactca aagcttataa gaccggcatt gtaaaggaca atggtgaggc
     2461 ctactgtggc actgctgctg tgaaccaaca tggtggttat catcccaagg ataagaccga
     2521 ttaacgggtt ttgcccatag gctttaaaga gttcgatggc tattcggctt tcctttatgt
     2581 aaggtactcc tgaattcagt atgttaagtc ctgtaaacat aagtccaagg ccggtcactg
     2641 cccatccgat gtttttgacc gaattttttt tcgatctgaa agcggtaaga agaccaaagc
     2701 cgataatgat ataacctgca tggggaagct tgaaggacat aagctgggca gttattgtag
     2761 ttccgatatt tgcaccgtaa attatgccta cggcctgagt gagattcaaa agccccgagt
     2821 ttacaagccc gacagttata accgttatgg ctgtactgct ttgaaccagt gccgtaagaa
     2881 ttgttcccgt tacaagggca aggggtaaat tgtttgtcaa ggcttcaagg caccatttca
     2941 ttctgtgggt gtttgctttt tccaatcctg tgctcattaa gttgacccca tatattatta
     3001 ataccgttcc aactatgaaa tttacagcga ttataataat tctgatatcc attttgcttt
     3061 atttttcctt atttaagaat gtatcttact tttaaacata agctttatcg tgcaaaaata
     3121 gaacaaccaa acatgaaaaa ctgcatagga attgaacgaa attttattag tataaagaat
     3181 aaaaaacaat catagtttaa ttgaaggaga tataatgaga aaaagggtgg acattttgtc
     3241 ggcttttaca agtaatatta tctacaattt tctaatatcc tttggcgtaa tagtgggggc
     3301 aagcctgtta gccgggattg gagcattgat aaacgatcat cctcctttaa gatgtatgtt
     3361 tgaaatagcg agctctataa aaatatgggc ggtagcggtt gctttgggcg gcaccttttc
     3421 aacctttgaa attcttgaaa aaggattgtt ccgcggagaa atcagatcga ttgttaagca
     3481 ggcggtatat gtggtagttg ctgttattgg ggccaatatg ggttatggct ttataaggct
     3541 tatccaaaaa tgtggggagt caatgataaa atgaagaaac ttgcatatag attgtttatc
     3601 tgctttatta cagggctttt aggaggaatt cttattggat gtaccgtgat gagtaccctt
     3661 gtcagctaca ggattgaccg gtatcatgag gaaataatgt ttcttaaaac cgttatagca
     3721 gataatgatg taaaattaaa aaagcttgaa gaatcctttg ataatgttaa taaaaagaag
     3781 tttcttgttt cagatataga ggttcacttg atttatgcgg atggcgagga aaacagtttt
     3841 gataaaatgg agctgacaaa ttatataaaa gataagtata aagacctttt gtggaaggag
     3901 gtaagaggta ttgataccga ccttgcggta gggcttatag ataatgatgt tttggtgatt
     3961 gaaggcaaaa gctatgaatt gagagtttcc agggtactta tatccgatgt attcaagata
     4021 tgggtaacgg taaagaataa ccaataattt cttttacttc attatttcac ttcatgaaac
     4081 aaatcaaaat aaattctttt tgagttgtaa cttttcaaac actaaatacg aataaataat
     4141 gaggaatgca ggttttaatg tattttactt taattatata taatatacaa tgggttttta
     4201 gtacctgacc ggtcaaagac aagggggtgt atgatgagga cgaaagagca taaccgggat
     4261 tgctatttta acgatctgga atcctttgag acaattgtac ttaagtaccg tgagaattca
     4321 attaattttg ccaaaagata tataaacgat attcaaattt gtgaggatat agcacaagaa
     4381 gcgtttgcag atgtgtatgt caatagagat aaatacaatc ccaagatatc ctttaagaca
     4441 tggttgtttt caatcataca taacaaatgt gtggattaca taagaaaaaa taaggaactg
     4501 ataatcgaca gtattgaaga taaatcccgg gaaggaaatg agtttgacaa tcttgaaaca
     4561 aaaatacttc tagaacatga aatcggtaaa cttaaatcaa attacagggc aataatccag
     4621 atggttgaag tcgaggggat gacaatccga gaggcggcaa aagttcttca aataagcgaa
     4681 gttcatgccc gggtattaaa tcacagggca aagaaaaaac ttcgcaagga attgatgaaa
     4741 ggaggatttg tgtatgaatg accgggagtt tttagatggg gtttacagga aaataaatat
     4801 tcttgaaaga gaacgcatgg aaatggaact tatccgaaag agacagaaga gactctttta
     4861 cagacaagta aagatgtgcc ttataccttt tttatcggtt gcagtacttg ttatattcct
     4921 attgttattc gaaatcagta tgttggagac tgctgttatc tcagcttcga tattgctgtt
     4981 catggtttgg ctggaggctt gtgactttga aatggaaaat actgatgtgg gagtgaggtt
     5041 aaatgagtat aattattaat aatctatgca aaaagtatgg caataaagtg gtgttggata
     5101 acgtaaattt gcagattgac aagggtatgt tcggactttt aggccctaac ggtgcaggta
     5161 aaaccacgtt aatgaggatt ttgacaactc ttattccccc tacttcagga acgatagaag
     5221 taaatggtgt tcagatacat agacgaaaag aaataagaga aataaccggt taccttcctc
     5281 aggagttttc cttgtatcct tctttttcgg tatatgaagc tttggattac cttgcggttt
     5341 tgtcccggat aacagacaat aagatgcgaa aagacattgt taacgagctt ttggaaaagg
     5401 tgaatttacg tcagtacaaa aaagtaaagg taaaaactct gtcgggaggt atgaaaaggc
     5461 gtttgggaat agctcaggca ctacttgcaa acccaaaggt attaattgtg gacgagccaa
     5521 cagccgggct ggatccggag gaacgtatac gctttcgcaa tatgcttggg agtttatctt
     5581 cagaacgtat agttatactg tctactcata tagtagaaga tattgagtcc acctgtgaaa
     5641 aactggcggt attaaaggaa ggaaaaattg tatatgcagg taccgtcgga gaattgctga
     5701 agtcggtcga aggatatgta tggagcggat tgatagagcc tgacaagcta aactttgttc
     5761 aggataattt tacgcttata tcatcggtaa gagaaaccga gaaattcagg gttaaaatat
     5821 tggcctccga aaagcccttt gaaggggccg aaaacatatt accgtctatt gaagacggtt
     5881 atatgaaaat catcaagggg gtgtaaatat gttattgaaa ctgtctgtta ttgaaataaa
     5941 acatcaattt aaaagaataa cgttttatgt atttgttttg ctgacagctc tattcgtatt
     6001 tactcaaatc ggagaattcg aaatgtggtc caaacatcct gatgaacagg aggacatata
     6061 tttattagca gatcaaatga tcggcgatcc caaacagcct gacgaaatgg caactcagaa
     6121 gggtgtatat gaattgctcc aaagagcggt tgaaagcaaa aaaataatgg ttcaagggtt
     6181 aataattaac aaggagaaaa gactttcgga ggaacagata aaactaatta aagaagtaat
     6241 gcaaaagata tctccggagg gtaaagaaat atggacagag gaggatctgc atctttccgg
     6301 tgaagagttt gagaaaatca gactgatcct agggtacagc gataatgatc caa
//
LOCUS       DAOOJC010000093         6260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_10244_length_6260_cov_4.017405, whole genome shotgun sequence.
ACCESSION   DAOOJC010000093 DAOOJC010000000
VERSION     DAOOJC010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10244_length_6260_cov_4.017405"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PLA01_03890"
     CDS             complement(<1..554)
                     /locus_tag="PLA01_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPD00474.1"
                     /translation="MAKILICDDEAGLRAVIKRYALFEGHKVTEAGDGIEAVELCRNN
                     AFDIIIMDIMMPELDGFSAVKEIRKFSDTPVIMLSARGEEYDKILGFEVGVDDYVVKP
                     FSSKEIMLRINAILRRVEKGSSAKKDAENHVIFEKDGFTADLTAYKVFIDGVQVEMAP
                     KEYDLLFFLIRNRNIAVPREKIME"
     gene            complement(603..>986)
                     /locus_tag="PLA01_03895"
     CDS             complement(603..>986)
                     /locus_tag="PLA01_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00475.1"
                     /translation="FPGGFGNIDMEAMRKAGEIIMQANGQITDEVKEKLKSIGLTEEQ
                     INQISSMTGGRFGGMGQNRGNNSVSDNREGMESKEQNRRFPGGQSTGGFLNRRTENNN
                     IIFMGMMSFVLFAAIIFAARFRKTY"
     assembly_gap    987..996
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<997..2507)
                     /locus_tag="PLA01_03900"
     CDS             complement(<997..2507)
                     /locus_tag="PLA01_03900"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPD00476.1"
                     /translation="MINSKGINVIVAIAVAFAVVLSAALVVAGNMQKASGSLKSEPEY
                     ANKVFGTDIISIDILADEEDWQEMLDNARSEQFIMVDVVINGTRFKNVGIRPKGNSSL
                     TQVASSDSDRYSFRLQFDEYIKDQTCFGLESMVLNNMLGDSTYMKEYVSYDLMREIGV
                     DAPYFGYADIKINGESHGLYLAVELYNDSYEQRVFGNTSGMLYNVKSMDMGNWDGNVP
                     GAPGGGMPSMFEGGMPGMFGNFGDRGWPGGSRGGGSLEYTDDNIESYSSIFNNVVGKG
                     KESDYQRVIQALKALSEGKELEKYFDVDKILRYLAAHTFVVNLDSYSSSMAQNYYIYE
                     RDGKLTVLPWDYNLAWGGFQNGDATSVINFPIDTPVSGVEMSSRPLIEKLFSNAEYLE
                     RYHSYLQQLIGKYLANGKFEGKIKSLDAVISEYVKNDATAFCTFDEYQNAVSAFITLG
                     NLRAQSVQGQLDGTVPSTTEGQKANPEKLISSGDLKLSVLGSMMGGRGGRDNT"
     gene            complement(2534..3205)
                     /locus_tag="PLA01_03905"
     CDS             complement(2534..3205)
                     /locus_tag="PLA01_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HPD00477.1"
                     /translation="MTFKDIFKTSFLEKAVEFSIVDVLTAMILAFAIGLFIFFVYKKT
                     FAGVMYSASFGVSIIAMTMITTLIILAVTSNIILSLGMVGALSIVRFRTAVKEPLDIA
                     FLFWAISAGIVIGAGLIPLGIIGSVLIGIVLLVFVNKKSSDTPYILVLKLENDKAESD
                     CMAKIKEKTKKSLIKAKTVSQNGIELTVEVRLLDMSATLLNELLAIKGVNNACLVSYN
                     GEYTA"
     gene            complement(3223..3900)
                     /locus_tag="PLA01_03910"
     CDS             complement(3223..3900)
                     /locus_tag="PLA01_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HPD00478.1"
                     /translation="MAETQGRHEIKHYINYSDVLQIRARLPFVAHPDKNAKEENSYRV
                     KSLYFDNYNDKALKEKIDGINGREKFRLRLYNDDPSFIRLEKKRKNNNICFKESCVIT
                     EEECNRLLDGDLDVLQENGNSLCLELYAKMYYQQLRPKNIVDYRREAYIYPMGNVRVT
                     LDYDIRTSYNIHDFLRSGPVLIPVSGVYILEVKYDNFLPEIIRGMVSLSGRRSTAFSK
                     YAITRIL"
     gene            complement(4081..>6260)
                     /locus_tag="PLA01_03915"
     CDS             complement(4081..>6260)
                     /locus_tag="PLA01_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPD00479.1"
                     /translation="GVAVFAAGAIILSTGTQNAQTKMLKSGLIAQETLLNADRDLYQA
                     YTAVQELVLMDSSIEETDNQIAFFNDNINTAKERVLQARKEMDATKKEWIEFKGAASN
                     LTAFELFERVNSGIDEWVQISRLSIENNAFDKGWKEAFNESRESLDEITELLSVAINE
                     NEAKYKSERTTVLINISIGLLIILAIITLFGYSIIRNISKPLGSVVEMVEEFEKGHLK
                     KRLNLNRKDEIGRLANTMDNYADYLQGQVVSSMDKIAEGNFDFEILIKDDKDEIGPAL
                     KKTVESIKGLVHEVSALTSAAIEGKLQVRGNEENFKGLYRDIISGINNTLNAAIEPIN
                     EAASVLGEVEKGNLQVSVKGDYKGEHAIIKEALNKTISNLKTYTNEISYVLNQMSEGN
                     LDIVVDKEFEGDFIEIKNSLNNILSVFNNVLNKMGEAALQVYAGSKQVADSSQILSQS
                     TTEQASSVQQLTAVMSEISEQTKRNAHNAKEASELTMAVKDDAVAGNERMNEMLKSMD
                     EISVASTNISKIIKVIDEIAFQTNILALNAAVEAARAGQHGKGFAVVAEEVRNLAGRS
                     AESAKETAVLIEKTVSKTENGMSIAKDTAEALSKIVSGVTRVTDYVSEISASSNDQAS
                     GITQVNQGVLQISQAIQMNSATSQQSAASSQELTSQADTLNEMVKRFKIRKNGYKKSG
                     YESIDEGILGLLEAASENHEVDSKEKYLNKKNIFTGDMNFGKY"
BASE COUNT         1686 a         1485 c          998 g         2081 t
ORIGIN      
        1 tcttccatta tcttttcccg tggaacagca atgtttctat tccttatcag aaaaaacaaa
       61 aggtcgtatt ccttaggtgc catctctacc tgcactccat caatgaacac cttgtatgcc
      121 gttaagtctg ccgtaaagcc gtccttttcg aaaatgacat gattctctgc atcctttttg
      181 gcagagctgc ctttttccac tctgcgtaaa atggcattaa tcctgagcat aatttctttt
      241 gaagaaaagg gtttgacaac ataatcatcc acaccaactt caaaacccaa aatcttatca
      301 tattcttcgc cccgggcaga aagcattatt accggtgtat ctgaaaattt gcgtatttcc
      361 tttacggcag aaaaaccgtc aagttcgggc atcataatat ccataatgat tatatcgaag
      421 gcattattac ggcaaagctc cactgcttca atgccgtccc cggcttccgt gaccttatga
      481 ccttcaaaca aggcataacg ctttataaca gcacgaagac ccgcttcgtc gtcgcatatt
      541 aatattttag ccattctata atcattcctt cctacttatt atgaatattc ttgcggcttt
      601 tactaatatg tttttctgaa ccttgctgca aatattatag cagcaaacag aacgaaagac
      661 atcatcccca tgaaaataat attattattt tctgttcttc tgtttaaaaa tcctcctgta
      721 ctctggccac cagggaatct tctgttttgt tcttttgatt ccatcccttc cctattatcg
      781 gaaacactat tgttacctct gttctgtccc atccctccga aacgtccgcc tgtcatcgaa
      841 gaaatctgat taatctgctc ctccgtcagg ccgattgatt tcagtttttc ctttacttca
      901 tccgtaattt gaccgttcgc ctgcattatt atttctccgg cctttctcat agcttccata
      961 tctatattac caaaacctcc ggggaannnn nnnnnnaaag tattatctct accacctctt
     1021 cctcccatca tactgcccag gacagacaac ttcaaatccc cggatgatat aagcttttca
     1081 ggatttgcct tttgtccttc ggttgtagag ggaactgtac cgtcaagttg accctgtaca
     1141 ctttgggctc gaagattgcc aagggtaata aatgctgaaa cagcgttttg gtactcatca
     1201 aaggtacaaa atgccgtagc atcgtttttc acgtattcgg aaattacggc atcaagggat
     1261 tttatttttc cctcaaactt tccgttggct aaatatttac caatgagctg ctgcaagtag
     1321 ctgtgatatc tttccaaata ctctgcgtta gaaaacagct tttctatcag aggccggctt
     1381 gacatttcaa cgccgcttac cggagtatca atgggaaaat taatcactga agttgcatca
     1441 ccgttttgaa aaccacccca tgcaaggtta tagtcccacg gcaaaacggt cagcttaccg
     1501 tcacgttcat aaatataata attttgtgcc atgctagaac tgtagctgtc aagattaaca
     1561 acaaaagtat gtgcggccag atacctcaaa atcttgtcga catcaaaata tttttccagt
     1621 tcttttccct cagaaagtgc tttaagggcc tgaataacac gctggtaatc gctctcttta
     1681 ccttttccca ctacattgtt aaaaatagag ctgtagcttt caatattgtc gtctgtatat
     1741 tcaaggctgc cacctcccct gctgccgccg ggccagcctc tgtctccgaa attaccgaac
     1801 atgccgggca ttcctccttc gaacatactg ggcattccac cgcccggtgc tcccggaacg
     1861 tttccatccc agtttcccat atccatgctt ttcacattat aaagcattcc agatgtatta
     1921 ccgaaaaccc gctgttcata actatcgtta tacaactcca ccgcaagata caacccatga
     1981 ctttcgccgt ttattttaat atccgcatat ccgaaatagg gtgcatcaac accgatttcc
     2041 cgcattaggt cgtaggaaac atattccttc atgtaagtgc tatccccaag catgttattg
     2101 agcaccatgc tctcaagccc gaagcacgtc tgatctttga tgtattcgtc aaactgcagc
     2161 cgaaaactat atctatcgct atcggagctt gccacttggg ttaagctgga gtttccctta
     2221 ggacggatac cgacattttt aaatctcgtt ccgttaataa ccacatcaac catgataaat
     2281 tgctcacttc tggcattgtc gagcatttct tgccaatcct cttcatcggc aagaatatca
     2341 atggaaatga tatcggtacc gaaaaccttg tttgcatatt ccggttcgga tttcaaactt
     2401 ccgcttgcct tttgcatatt gccggccacc actagagctg cacttaatac cacggcaaat
     2461 gcaacagcga tggcaacaat aacatttatt cctttactat taatcatttg cagtcacctc
     2521 aaatcctgca tgttcaggct gtgtattcgc cgttatagct tacaaggcat gcattgttca
     2581 cgccctttat ggcaagtaat tcattgagaa gtgttgccga catatctaaa agcctcactt
     2641 caacagtaag ctcaatgccg ttctgggaaa ctgtctttgc cttaattaag cttttcttgg
     2701 ttttttcctt tatttttgcc atgcaatcgc tttctgcttt atcgttttca agtttaagta
     2761 ccaagatgta tggagtatcg ctgctttttt tgtttacaaa cacaagaaga acaataccga
     2821 tcaaaaccga accgataata ccgagaggaa ttaaccctgc accgataacg attcctgccg
     2881 aaattgccca aaataaaaat gcaatgtcca gaggctcttt aactgctgtt ctgaaacgaa
     2941 ctatggacag tgcgcctacc ataccaaggg acaggataat atttgacgtt accgcaagaa
     3001 taataagggt tgtgatcatc gtcatagcta taattgaaac accaaaggat gccgaataca
     3061 taaccccggc aaaggttttt ttgtatacga aaaagatgaa aagcccgatt gcaaaggcca
     3121 gaatcattgc cgtcagaaca tccacaattg aaaattccac agctttttct aaaaaacttg
     3181 ttttaaaaat gtctttaaat gtcattgttt gtaaccccca atttataaaa ttcttgttat
     3241 tgcatatttg gaaaatgcag tacttcttct gccggaaaga gataccatac cgcggattat
     3301 ctccggcaaa aagttatcat atttcacttc aagaatataa actcccgaaa ccggtataag
     3361 tacaggtcca gatcttaaaa aatcatgaat attgtagctt gtccgaatat cataatccaa
     3421 ggtaaccctc acattcccca taggataaat gtatgcttcc cgcctataat ccacgatatt
     3481 tttcggccga agctgctgat aatacatttt tgcatacagc tccaagcaca gagaatttcc
     3541 gttttcttgt aatacatcaa ggtcgccgtc aagcaaccga ttgcattctt cttccgttat
     3601 gacgcagctt tccttaaagc aaatgttatt attctttctc tttttttcaa ggcggatgaa
     3661 ggatggatca tcgttataaa gacgtaaccg gaacttttcc ctcccgttta tgccatcgat
     3721 tttttctttc agagctttat cgttatagtt gtcgaaatag aggctcttaa cccggtagct
     3781 gttttcctct ttcgcatttt tatcaggatg tgctacgaag ggaagccttg ctcttatctg
     3841 taatacatcg gaatagttta tatagtgctt tatttcatgt ctgccttggg tttctgccat
     3901 tctctcactc ctttcctccg gaatgatttt attttaagca atgaaagtga aatgcaattg
     3961 tatgttatct gaactttatc tgaattttgg gtaagcaaaa aaccgtccct gttctatgga
     4021 cggtcatgtt ccaaattgaa aaggcatgaa gcaagtatta aatgaaatag ccgctatgtc
     4081 ttaatacttg ccgaaattca tatcaccagt aaatatattc tttttattta agtatttctc
     4141 ttttgaatcg acctcatggt tttcgctcgc agcttccagc aatcctaaaa tcccctcatc
     4201 aatgctttca tatcccgatt ttttatatcc attttttctt atcttaaacc ttttcaccat
     4261 ttcgttaagg gtatctgcct gacttgtcaa ttcctgactt gaagcagcac tttgctgcga
     4321 agttgccgaa ttcatctgaa tagcctgcga aatctgcaac acgccttgat ttacctgcgt
     4381 aatccccgat gcctgatcat ttgatgaagc cgatatttca ctaacatagt cagttactct
     4441 tgtaactccg ctaacaattt tagaaagtgc ttctgcagta tcttttgcaa tactcatacc
     4501 attttccgtt ttcgaaacgg ttttctcaat tagaaccgcc gtctctttgg ccgattctgc
     4561 acttctgccg gcaaggtttc tgacttcctc agccacaact gcaaaaccct ttccgtgctg
     4621 tcctgccctg gctgcttcaa cagcagcatt taatgcaaga atattggtct gaaatgctat
     4681 ttcatctatc actttaatta ttttagatat gtttgttgat gctacagata tttcatccat
     4741 tgatttcaac atttcattca tccgttcatt accggcaact gcatcatctt tcactgccat
     4801 agtaagctca ctcgcctctt tggcattgtg tgcattcctt tttgtttgtt cggatatttc
     4861 actcataact gccgttaatt gttgaactga acttgcttgt tctgtggtac tctgcgaaag
     4921 tatctgggat gaatccgcaa cttgtttcga acccgcatac acctgtaatg ctgcctctcc
     4981 catcttgttg agcacattat tgaatacaga taaaatatta ttgagagaat tttttatctc
     5041 aataaagtca ccttcgaatt ctttatccac cacaatgtct aaattacctt ctgacatttg
     5101 attcagcaca tagctgattt catttgtata agtttttaaa ttggagattg tcttgtttaa
     5161 tgcctccttt attattgcat gctcaccctt atagtctcct ttaacactaa cctgcaggtt
     5221 tcctttttct acttctccaa gtacgctggc tgcttcattt atcggctcta tcgcagcatt
     5281 gagcgtgtta tttattcctg aaataatatc tctgtacaat cctttgaaat tttcctcatt
     5341 tccccttact tgaagcttac cttcaattgc agccgaagtt aacgcactca cttcatgaac
     5401 aagtcctttt attgactcga ctgtcttttt taatgcagga cctatctcat ctttatcatc
     5461 ctttatcaaa atctcgaaat caaaattccc ttccgctatc ttatccattg aacttacaac
     5521 ctgcccttga agatagtcgg cataattatc cattgtatta gcaagccggc ctatttcatc
     5581 ttttctgttt aagttaagcc ttttcttaag atggcctttt tcaaattcct caaccatttc
     5641 tactaccgaa ccaaggggtt tggatatatt tctaattatc gaatatccaa acaaagtgat
     5701 tatagccaat ataatcagta atccaataga aatgtttatt aaaacggtag ttctctcact
     5761 cttgtacttt gcttcatttt catttatagc tactgaaagc aactctgtta tttcatccaa
     5821 actctcacgg gattcgttaa atgcctcttt ccaaccttta tcaaaagcat tgttttctat
     5881 agataacctt gagatctgaa cccattcgtc tataccggag tttactcttt caaataattc
     5941 aaatgctgta agattgcttg cagctccttt aaattctatc cattcctttt ttgtagcatc
     6001 catttccttt cttgcttgta aaaccctctc ctttgcagta ttaatgttgt cattaaaaaa
     6061 agcaatttga ttgtcagttt cttctattga gctatccata agaaccagct cttgaaccgc
     6121 tgtatatgcc tgatataggt ctctgtcagc atttaataat gtctcttgtg caataagtcc
     6181 ggatttcagc atttttgttt gtgcattctg agttccggta ctcaaaataa ttgcccccgc
     6241 tgcaaataca gcgacgccaa
//
LOCUS       DAOOJC010000094         6225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_10311_length_6225_cov_3.544895, whole genome shotgun sequence.
ACCESSION   DAOOJC010000094 DAOOJC010000000
VERSION     DAOOJC010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10311_length_6225_cov_3.544895"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1627
                     /locus_tag="PLA01_03920"
     CDS             134..1627
                     /locus_tag="PLA01_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00480.1"
                     /translation="MSIKKKKTVISDDKIIILDYLDYFTEKFLLLILLIVFGPILIQV
                     ISTGYLPIILILAAIFILAISFTLYERFKKNKSNIQEGYEYIIDKSSQTLIIKKTEYF
                     KFKKYEYSIKGNVKLKITDNFDNEENKPYYNLYISAGEKEILIQQAYNENFIKELVDE
                     IIRLLNIPHSIIEDPKGILFNESISLPIKGISDYDKTESKEVLEDEIIKNRHPKDSFL
                     NAGEEYDDAIKRKILLEKIDEINEKDKNHSTPLLEGECKAKVYDRKLIINVRRNYILF
                     FAVLALFLFLIYKFSLVETVILERTGSNVINCVIKYNFLGIEKLEAEKYILNDLKNFI
                     TKTDSEGDITYTFEYQNNKRFNASLFNANSEDVEAIESFLKDESEKKLIIQGKQHFSS
                     ILFNCFLALNALLLIISSIEILLKRKIRKMVIDGDGKKILVTFKSIIKNEEMEYAISI
                     LDSIEIKKISDKDYLYLKMLNRSLNVGQIKNKNNLYNIEMFINNLFS"
     gene            1899..3833
                     /locus_tag="PLA01_03925"
     CDS             1899..3833
                     /locus_tag="PLA01_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00481.1"
                     /translation="MVEKLKVPSEKILILLLNRTQSLEWRTRTVLEGSGAILRTSYYG
                     FIQGEIKTYYPIILKNCKGIVKKDIKPVFLTFEAAQFLVSIVLEWRRQNKGYFSGVTS
                     FSDRLAIDMTSNLVRAAISDIPYNEIGKRLYDALEKKEEIKKQVYSEADDILSAYRKR
                     CMELGIFDFGMAVELYNKYLITDNTYREQLFKRIDHLIVDNIEECVPTEVDFINFLLP
                     RLKTCLMAYNHEGGYGEAFGSNHEYMKQKLLGRIEMVELNKSFTCKDYMYEFSDMLFD
                     AIENGTFKSCKPGKEIERVMPFELRSEMLEKAGERIVSLIEDEGYSPSDIAVISYYAD
                     PVSEFVIARCLEKHGYEIKNLARKSRIIDNPFSQALITLAQLCHPSYGLVPGRDDVRA
                     LLRMVLKIDPVRSSVLAGEILAQRPFAEFPDIEFPGLVERVGYYNIEKYEHIRNWIKE
                     YREMEKPLDINEFFQKVFLEILISTEVSQKDILETRKLIDSAKSFVDVVLRFNRNANR
                     DFLEVVRRGIKSGESIFELEEKHSGNFVLMSTPVAYLAGSLKSKVIILLSLSSSNWTP
                     RSIKEITNIHVLTKTWDVNEIYTEEMEEKNQKHYLAVLIRSLFKRCGEKLITFESALS
                     ANGYENDGILADYFDEILSQ"
     gene            3950..6187
                     /locus_tag="PLA01_03930"
     CDS             3950..6187
                     /locus_tag="PLA01_03930"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPD00482.1"
                     /translation="MGLRKGQKELVERYRGGYCAIPAIPGGGKTHCLTLWVAEMISNG
                     LHKPGKILIVTYMNSAVNNFKKRISEELQKRGISTNKDYFVSTIHGLCLQIIKEKPDQ
                     IFANEEFDVVDGVVKNHIISAAVDEWKRHNERAFKLYLDIESLSAERIGDVYRNWHDK
                     LCGIMLSAISDFKIRGITPQIAKEMCRECGRDSIIFWASQIYEIYDRKLKMSGALDFD
                     DMLYNAKKMLQEDEHLLEKYRKKYSFVCEDEAQDSNYIQNEILKMIANGNLLRVGDSN
                     QAICGSFSSSDFTLFKSFCEHPDTTVYRITQSSRNTKDIIDLANYFVKYVRERHPVEE
                     CRESLLPQYIEPVDENDSRPNPRIEEYGIKAAVVSSWEDEARAVVNQAIQMMNKYPEK
                     TIAILVPTAWRMAFVLNCLEARRVPFEQLDNTSDEKNRTVRKLGRVIDFIAVPEVGEK
                     LWDMMRDCFLTDVYDKPDLDTSGEEPKKAGQKELLERFLKRYPVEKLLYPLGGEIEIS
                     EIPQELIKTRIWKDFLDQLETVRELLEFPNTIVEKLILFISEKLRFDREERAIAQKVA
                     GDVRFLIGGTSGWRLNDLALELLSPKNMFNYFAGIVWDLKGYEPRPGVVTLSTYHKSK
                     GLEWDIVFLTGLNNSDFPVLLEDRFIGEYWFLKEGYKNPQALLKKEMEKLWDRKTKGD
                     PVKETRLETISERARLLYVGITRAKEYLFLSSFEQNQGKRNEILPSKYILELRKFIEE
                     AEKKC"
BASE COUNT         2129 a          990 c         1396 g         1710 t
ORIGIN      
        1 tgtgacactt ggcaaaaaca ttgtaaagaa gggtttgttg catcgtatga tgcaacataa
       61 acctaaggat ataccgatat aagtttttcg tagaggttac catacatcta cctatcaaaa
      121 ggagggcgat gtcttgagta ttaaaaagaa aaaaacagta ataagtgatg ataaaatcat
      181 tatattagat tatttagatt attttactga aaagttttta ttgttaatat tactaattgt
      241 ttttggacct atactaattc aagtcatcag taccggatac cttccgatta ttcttattct
      301 ggccgccata tttattcttg ccatatcttt tacattgtat gaaagattca aaaagaataa
      361 atcgaatata caggaaggat atgaatatat tattgataaa tcttctcaaa cactgattat
      421 aaagaaaaca gagtacttta agtttaaaaa atatgaatat agcattaaag gaaatgtaaa
      481 gttgaaaatt actgataact tcgacaacga agaaaataaa ccctattata atttgtacat
      541 cagtgccggg gagaaggaaa tattaattca gcaagcttat aatgaaaatt tcatcaagga
      601 gttggttgat gaaataatac gtcttttaaa tattccccat tcaattattg aagatccaaa
      661 aggtatacta ttcaatgaat ccataagttt gcctataaaa ggcatttctg attatgacaa
      721 gacagaatca aaagaagtct tggaagatga gattataaaa aaccggcatc cgaaagacag
      781 ttttctaaat gcgggtgaag agtacgatga tgcgataaag agaaaaattt tattagaaaa
      841 aatagatgaa ataaatgaga aagacaaaaa tcattcgaca cctcttttag aaggagaatg
      901 taaagctaag gtatatgata gaaagctaat aataaatgtt aggagaaatt atatactttt
      961 ctttgcagtt ttagctttat ttctatttct aatttacaag ttttccttgg ttgaaaccgt
     1021 aatcttggag cggacaggtt caaatgtgat aaattgcgta attaaatata attttttagg
     1081 catagaaaag ctggaggctg aaaagtatat tctcaacgat ttaaagaact tcataacgaa
     1141 aactgacagc gagggagata tcacatatac attcgagtac cagaacaata aaaggtttaa
     1201 tgcatcatta tttaacgcta atagtgagga tgttgaagca atagaatctt tcctgaaaga
     1261 tgaatctgaa aaaaaactca ttatacaagg aaaacaacat ttttcaagta tattatttaa
     1321 ctgttttctg gcattaaatg cattgttatt gataatatca agtatagaga ttctattgaa
     1381 aagaaaaata cgaaaaatgg taatagatgg ggatggtaaa aaaatccttg taacctttaa
     1441 atccattata aaaaatgaag agatggagta tgccataagc attttggata gtattgaaat
     1501 taaaaagata agtgataaag attatttgta tttaaaaatg ctaaataggt ctttaaatgt
     1561 tgggcaaata aagaataaaa acaatttata taatatcgaa atgtttataa ataacctttt
     1621 cagttagatg ccgcaaacga agcagaattt cgggataata caggcatact gttctttgaa
     1681 agtattacat gaaccttgat aagactattg atgttttcat taattatccc acaatggtat
     1741 aatatactta aataccgatt atcggaggga tacatattat aatttccaat gacctcgcag
     1801 ataaaataat agaagcaccg gcagaggatt cgaagattat actcaaaggt cctacgggaa
     1861 gcggaaaatc aaccctgctt ctcgaaaggt ataagtatat ggttgaaaag ctgaaggtgc
     1921 ccagtgaaaa aatattgata cttctgttaa acagaaccca gtcccttgag tggagaacgc
     1981 ggactgtgct tgagggctcc ggtgctattt tgaggacctc ttattacggt tttatccagg
     2041 gagagataaa gacctattac cctattattc ttaaaaactg caagggaata gtaaaaaaag
     2101 acataaaacc tgtgttttta acctttgaag ccgcacagtt tttggtttcc atagttttgg
     2161 agtggcgtag gcagaataaa gggtatttct ccggggttac atcctttagc gacaggcttg
     2221 ccatagatat gacatccaat cttgtccggg cggctatttc ggatattccc tataacgaga
     2281 taggtaaaag gctctacgat gcactggaga agaaggaaga aataaaaaaa caggtgtaca
     2341 gtgaagcgga tgatatttta agtgcatacc gcaaaagatg tatggagctt ggcatatttg
     2401 acttcggcat ggctgtagag ctctacaata aataccttat cactgacaat acataccggg
     2461 agcagctgtt taagagaatt gaccacctga ttgttgacaa tattgaagaa tgtgttccga
     2521 cagaggtgga ctttataaat ttccttttgc cgcgtctaaa aacctgctta atggcataca
     2581 accacgaagg gggatatgga gaagccttcg gaagcaacca cgaatacatg aagcagaagc
     2641 ttttgggcag gattgagatg gtggaactaa ataaatcctt tacatgcaag gattatatgt
     2701 atgagttttc ggacatgctt tttgatgcaa tagaaaacgg gacatttaaa agttgtaagc
     2761 ccggaaagga aatagaaagg gttatgccct ttgagctaag aagtgagatg ctggaaaagg
     2821 cgggcgagag aatagtatcc cttattgagg atgaaggcta cagtccttcc gatatagcgg
     2881 tcatttccta ctacgcagat ccggtatctg agtttgttat agcacgatgc ctggaaaaac
     2941 acggctatga gataaaaaac cttgccagaa agagcagaat catagacaac cccttttccc
     3001 aggcacttat aaccctggca cagctctgcc atccgtctta tggtttggtt ccaggaagag
     3061 atgatgtccg ggcactacta cgaatggtgc ttaaaattga tccggtcaga agctctgtgc
     3121 ttgcgggaga aatacttgcc caaagacctt ttgcggagtt tcccgatata gaatttcccg
     3181 gccttgtgga aagggtggga tattataaca ttgaaaagta tgagcatata agaaattgga
     3241 taaaagaata cagggagatg gaaaaacccc tcgacataaa tgagtttttt cagaaggtgt
     3301 ttttggaaat ccttatttct accgaggtgt cacagaagga tatccttgag acgagaaaac
     3361 tcattgacag tgccaagtcc tttgttgacg tggtattgag gtttaacaga aatgccaaca
     3421 gagacttctt agaagtggtt agaaggggta taaaatctgg agaaagcatt tttgaactgg
     3481 aggaaaagca tagcggaaac tttgtgttga tgtcgacacc ggttgcttat cttgccggtt
     3541 cgttaaagag caaggttatc atactgttaa gcctcagcag cagtaactgg actccaagaa
     3601 gcataaagga aatcaccaat attcatgtgt tgaccaagac atgggacgta aatgagattt
     3661 ataccgagga gatggaagag aaaaaccaga agcattatct ggcagttctt ataaggtccc
     3721 tgtttaaaag gtgcggtgag aagctgataa cctttgaatc ggctttatcg gcaaacggat
     3781 acgagaatga cggtatcctg gctgactatt ttgatgagat actctcacag tagattaagg
     3841 tttataataa agggtgcaaa ttatatatgt gaagaaaaat gtggcgaaac ttgagcttct
     3901 gtaacacttt tcttcacacg tattcttaat aaattgattg gaggccttta tgggacttag
     3961 aaaaggacag aaagagcttg ttgaaaggta caggggaggt tactgtgcta ttcctgcaat
     4021 acctgggggc ggtaagaccc attgcctgac cttgtgggtt gcggaaatga taagcaatgg
     4081 tcttcataag ccgggcaaaa tacttattgt tacatatatg aacagtgctg ttaataattt
     4141 taagaagcgt atttccgaag aactccaaaa aagaggaatc agcaccaaca aggactattt
     4201 tgtgtctacc attcatgggt tatgtcttca gataataaag gaaaagcctg accaaatttt
     4261 tgcaaatgaa gaatttgatg ttgtggacgg tgtagtgaaa aaccacatta tatctgccgc
     4321 ggttgatgaa tggaaacggc ataatgaaag ggcattcaag ctgtatctgg atattgaaag
     4381 tttaagtgcc gaaaggatag gggatgtata ccggaattgg cacgataagc tttgcggcat
     4441 aatgctttct gccataagcg actttaaaat aaggggtatt accccccaaa tagcgaaaga
     4501 gatgtgccgt gagtgcggca gggactcaat tatcttttgg gcatctcaaa tttatgaaat
     4561 atacgacagg aagcttaaaa tgagcggggc cttggacttt gatgatatgc tttacaatgc
     4621 taagaaaatg cttcaggagg atgaacatct tctggagaaa taccgtaaaa agtacagctt
     4681 tgtatgtgag gatgaggccc aggattccaa ctatattcaa aatgaaatac ttaaaatgat
     4741 tgccaacgga aacctcctta gggtgggaga cagcaaccag gccatatgcg gaagctttag
     4801 cagcagcgac ttcacgctgt ttaaaagctt ttgtgaacat ccggacacta cggtgtacag
     4861 gataacccag tccagcagga atacaaagga tattatagat ctggccaatt atttcgtaaa
     4921 atacgtaaga gaaagacatc ccgtggaaga atgcagggaa agcttgcttc cccaatatat
     4981 tgaacctgtt gatgagaacg acagccgccc caacccacgg atagaggaat acggcataaa
     5041 ggccgctgtt gttagctcat gggaagatga ggcaagggct gtggtaaatc aggccataca
     5101 aatgatgaac aagtatcccg aaaaaaccat agccatactt gtacctactg catggaggat
     5161 ggcctttgtt ttaaactgtc tggaagccag aagggttccc tttgagcagt tggataacac
     5221 ctccgatgaa aagaacagga cggtaaggaa gctgggaagg gtaattgact ttatcgcagt
     5281 accggaagtg ggtgaaaagc tctgggatat gatgagggat tgttttttaa ccgatgtcta
     5341 cgataagccc gatttggaca caagcggaga agaaccgaaa aaagcaggtc agaaagagct
     5401 tttggaacgg tttttgaaaa gatacccggt ggaaaagcta ttgtaccctt tgggaggaga
     5461 aatagaaatt agcgagatac cgcaagaact cataaagaca aggatatgga aggatttttt
     5521 agatcagctt gagactgtca gggagctttt ggagtttccc aacaccattg tggaaaaact
     5581 tattttgttt atatcggaaa aacttcggtt cgacagggaa gagagggcaa tagcacagaa
     5641 ggttgccgga gatgtgaggt ttcttatagg agggacttcc ggctggagac ttaacgatct
     5701 tgcgttggag cttctaagcc ctaaaaacat gtttaattat tttgcgggaa ttgtatggga
     5761 tttaaaaggc tatgaaccaa gacccggggt tgttactctg tctacctacc acaaatccaa
     5821 agggcttgaa tgggatatag tattccttac gggacttaac aactctgact ttccggttct
     5881 tttagaggat aggtttatcg gcgagtactg gtttttaaaa gagggctaca agaaccccca
     5941 ggccttgcta aaaaaagaaa tggaaaagct gtgggaccga aaaacaaaag gtgatcccgt
     6001 aaaagagacc cggcttgaaa cgatttctga aagggcaagg ctgttgtatg ttggtatcac
     6061 cagggcgaaa gaatacctgt ttttgtcttc ctttgaacag aatcagggca aaagaaatga
     6121 gatacttccg tcaaaatata tcctggaatt aagaaaattt attgaggagg ctgagaagaa
     6181 atgttaagcg atttacgtta taccggatat ttcttgttta ctcag
//
LOCUS       DAOOJC010000095         6221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_10327_length_6221_cov_3.439183, whole genome shotgun sequence.
ACCESSION   DAOOJC010000095 DAOOJC010000000
VERSION     DAOOJC010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10327_length_6221_cov_3.439183"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2312
                     /locus_tag="PLA01_03935"
     CDS             <1..2312
                     /locus_tag="PLA01_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPD00483.1"
                     /translation="SNGDVAADVNADSLIDSTDLTIFKRYLLKKISEFPAGTTIGGGG
                     SPQIYQAEDAVFSKAVTETVNAGYTGSSYVNYDNEVGGYIEWTVNAQVSGAYELTFRY
                     ANGTSANRPMEISVNSNVVMSSLDFNSTSAWTTWHEQSIIVNLNQGGNKVRATAIVSE
                     GGPNVDYLQVAKSDGTPSTQKPTPTPTPTPTYGPTQTPKAGARQMEKLDRGVVAVKVN
                     NGVFISWRMFGTDPDNIAFNLYRNGTKVNSSPITGATNYVDSAGTTSSKYSVRPIING
                     QEQTASKEASVWAQNYLQIPIQPPSSAYDANDASCGDLDGDGEYEIVLKWEPKNAKDN
                     SQSGTTNNVILDAYKLNGTRLWRIDLGRNIRAGAHYTQFMVYDLDGDGKAEVACKTAD
                     GTKDGKGKVIGNANADYRNSSGYILSGPEYLTVFSGQTGEALSTVDYIPARGSVSAWG
                     DNYGNRVDRFRACIAYLDGQRPSLVMGRGYYTRMVFAAYDFRNGKLTHRWTFDSNTSG
                     NSSYAGQGNHNLAVGDVDGDGRDEIISGMACIDDNGKGLWNARLGHGDAMHFSDLDPN
                     RPGLEVWGCLESSGGAVLLDAKTGQQIFRWKASGDTGRCGAADIDPNTPGVEIWAAGS
                     SLFSCTGKNIGSAPSQKNFSIWWDGDECRELLDGTTITNRDGSGGGLSPSGVASNNGT
                     KSTPCLQADLLGDWREEVIWRTSDNRYLRIYTTTHTTQRRIYTLMHDPIYRLGVAWQN
                     VAYNQPPWSGFFIGAGMKEPPKPNIYLVP"
     gene            complement(2425..3375)
                     /locus_tag="PLA01_03940"
     CDS             complement(2425..3375)
                     /locus_tag="PLA01_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00484.1"
                     /translation="MKSELFSGLNDLKASNELKRKILLSIKNDSVPAKDKPNRSRLRI
                     ALVAGFVMLIIAGISVGYRLLETYRDSFQNENGSLVTQVKSSPSSNAKFEILDSPPQI
                     DCNFLMDYDPLSEGEVFRFADIIIFGQVKDFKWVKINSRNFIETLDTPNFYDYFRYQT
                     IATVKVIKAYKGDIEMDDTLEILLPGIVYGNKTVGNKIWAEDNTIAQNLEIDSRAFFF
                     ARDNTGKRIYKDTLGFDLEDICKYSTEWGEEFIIAEKDDRYIYSGMFKTLQSARDIYS
                     NIFEPSCTLGILENRSQSFECYIPTATFEEIEVMIEKYVK"
     gene            complement(3365..3862)
                     /locus_tag="PLA01_03945"
     CDS             complement(3365..3862)
                     /locus_tag="PLA01_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPD00485.1"
                     /translation="MTYGLSHTDDFILAILDKYTDMVFRLAFMYLRNHSDAEDATQEI
                     FLKLFRITPQFNNEDHIKAWLITVTSNYCKDILKSMWSKKVSLQHEMYLPIPDDNKRE
                     VIKQVMNLPQKYRDVIYLYYYEGYSSSEIAGLLSEKESTIRTRLKRGREKLKTKLKGV
                     FYDEE"
     gene            4082..4294
                     /locus_tag="PLA01_03950"
     CDS             4082..4294
                     /locus_tag="PLA01_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00486.1"
                     /translation="MGIKKLYQKRIEEFSGIVDRLDKASGLYSILRLVTFAVGTLLTI
                     LAYIYIGSIYGFIVMVLSVGIFVFLW"
     gene            4288..5886
                     /locus_tag="PLA01_03955"
     CDS             4288..5886
                     /locus_tag="PLA01_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPD00487.1"
                     /translation="MVKHQKIIDRAARYKRMVEINKRCIDRMEGSWTDFEDKGLEFAD
                     SEHIYAGDLDIFGHASLFQWINTAHTFFGRIALKNLFTSPSKDIEKIRKRQKAVKELS
                     DKIDFCQNLQCEGMGNIKASENPDKLVSYSEDNKKLFSEKHISQLFFILPELTIIFVI
                     LCRLDASVSIYIPLSLLTLQAVMSLIFYGRVSAVLRPVNKYKDRINVYRKMIEMIEKE
                     DFADEYLNELKSRFFLGKVSASAQIKGLERIVDAVDLGNGTIVAVILNLLLFWNVHCV
                     FALEKWKEKSGKSIKGWLETIGEFEALVSLSLVTQMNPEWVFPVISKNKVSFSAEKMG
                     HPLINEGNRVCNSINMDNRICIVTGSNMSGKTTLLRTVGINLVLAYAGTSVCAKEFMC
                     SVMDICTSMRITDDLNSGISTFYAELLRIKRIIDHSRKKSPMIFLIDEVFRGTNSLDR
                     VTGAKNVVLNLDRDWIVGMISTHDYELCKLGQKDGSRIINCHFTETYENNQIKFDYIL
                     RPGRSKTTNAKYLMKMVGIELLEQ"
     gene            5940..>6221
                     /locus_tag="PLA01_03960"
     CDS             5940..>6221
                     /locus_tag="PLA01_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00488.1"
                     /translation="MEKKKEIMEEDLYAPVKDYLTKQGYDVKGEVQNCDVSALKGETL
                     LVVEMKKTLNLDVILQATLRQRIADIVYIAVPKKSRVLFTKRWKDICHLL"
BASE COUNT         1919 a         1084 c         1408 g         1810 t
ORIGIN      
        1 tttcaaacgg ggatgtagca gcagatgtaa atgcggattc gctaattgac tcaacagatt
       61 taaccatatt taagagatat ctgttgaaaa agatatctga gtttcctgca gggacaacaa
      121 tagggggagg cggttctcca cagatatatc aggctgaaga tgcagtattt tcaaaggcag
      181 tgaccgaaac ggtcaatgcc ggttataccg gtagcagtta tgtaaactat gataatgaag
      241 tcggtggtta tatcgagtgg actgtaaatg ctcaggtttc aggtgcatac gagcttactt
      301 tcagatatgc aaacgggact agtgccaata gacctatgga aataagtgtt aactctaatg
      361 ttgttatgag cagccttgat tttaattcaa cttcagcatg gactacatgg catgagcagt
      421 ctattattgt taatctgaat caaggcggta ataaagtaag ggctactgca attgtttccg
      481 aaggaggacc gaatgtagac tatctacagg tagctaaatc cgatggaacg ccatcaacac
      541 aaaaaccgac acctacacca acacctacac cgacttatgg acctacacag acacctaaag
      601 caggtgcaag acagatggag aaacttgaca ggggagtagt tgccgtaaaa gtaaacaatg
      661 gtgtattcat cagctggcgt atgtttggaa ccgatcccga caatattgcg ttcaacttgt
      721 atcgcaacgg aactaaagtt aattcttcac ccattaccgg ggctacaaac tatgtggatt
      781 ctgccggaac cacaagttca aaatattctg ttcgtccgat tataaacggg caggagcaaa
      841 cagcatcgaa agaagcaagt gtatgggcac agaattactt gcagattccg attcagcctc
      901 catccagtgc ctatgatgct aacgacgcta gttgcggaga cctcgacggt gacggagaat
      961 atgaaattgt acttaaatgg gagcctaaaa atgcaaagga taattcacaa tccggtacaa
     1021 caaacaatgt aatcttagat gcttataaat taaacggtac tcgtttatgg agaatagacc
     1081 ttggaaggaa tatccgtgcc ggtgcacact acacccagtt tatggtttat gaccttgatg
     1141 gggacggaaa agcagaagtg gcttgtaaga ctgctgacgg aacaaaagac ggaaaaggaa
     1201 aagtgatagg aaatgcgaat gcagattacc gcaattccag tgggtacata ttgtcaggac
     1261 ccgagtacct cacagtattt agcggtcaga cgggagaggc tttgtcaacg gtggattata
     1321 ttcctgcaag aggaagtgtt tctgcatggg gtgacaatta cggaaacagg gtggaccgtt
     1381 tcagagcttg tatagcttat ctggacggac aacgtccgag tcttgttatg ggccggggat
     1441 actatacaag aatggtattt gcagcgtatg acttcagaaa tggaaaactt acacacagat
     1501 ggaccttcga cagcaatacc agcggtaact catcctatgc aggacagggt aaccacaacc
     1561 ttgctgttgg tgacgttgac ggagacggac gtgacgaaat tataagcggt atggcttgta
     1621 ttgacgacaa tggaaaaggc ttatggaacg ctcggttagg tcatggtgat gcaatgcatt
     1681 tcagtgatct tgatccgaac aggcccggtt tggaagtatg gggatgtctt gaatcatccg
     1741 gaggagcagt cttgttggat gcaaaaaccg gacaacaaat attccggtgg aaggcatccg
     1801 gggataccgg tagatgcgga gcagctgaca ttgatcccaa cactccggga gttgagatat
     1861 gggctgcggg ttcttcgctg ttcagttgta caggaaagaa cattggttca gcaccaagtc
     1921 aaaagaactt ttcgatttgg tgggacggcg atgaatgccg tgagctctta gacggtacta
     1981 cgataactaa ccgtgatggc agtggaggag gattatcgcc tagtggagtt gcttcaaaca
     2041 acggtacgaa atccacacca tgtttgcaag cggatttatt gggagactgg agagaggaag
     2101 taatctggag aaccagtgat aacagatatt tgagaatcta tacaactact catacgacac
     2161 aaagacgtat ttatacatta atgcatgatc ctatttacag actgggtgta gcatggcaaa
     2221 atgtagcata caatcagccg ccatggtcgg gcttctttat cggtgccggc atgaaagagc
     2281 ctccgaaacc gaatatttac cttgtaccgt aatcggatag gaaatgttga ttcgatgaag
     2341 gcaaaataaa tgtcaatgag tacgatttcc agttgataat ataaaaagta atggggaaag
     2401 tctaaaaggc ttttcccatt acttttactt aacgtatttt tctatcatga cttctatttc
     2461 ttcgaaagtg gcggttggaa tatagcactc aaatgattga ctgcggtttt ctagaattcc
     2521 tagagtgcat gacggctcga agatgtttga ataaatatcc ctggcacttt gcaatgtttt
     2581 gaacatgccg gaataaatat acctatcatc cttttcagca ataataaatt cttcgcccca
     2641 ttcggttgaa tatttgcata tgtcctccaa atcaaatccc aatgtatctt tataaattct
     2701 tttgccggtg ttatccctgg caaagaaaaa tgctcttgag tctatttcta aattctgtgc
     2761 aatggtattg tcttctgccc aaattttatt gccaacagtt ttatttccat atacaatgcc
     2821 cggaagcaga atttccaaag tatcatccat ttcgatatca cctttatatg cttttatgac
     2881 cttaaccgtt gcaattgtct gatatctaaa ataatcgtaa aagtttggcg tgtcgagggt
     2941 ttcgataaaa ttccggctat taattttcac ccatttaaag tctttcactt gtccgaagat
     3001 gataatatca gcaaagcgaa atacttcgcc ctcagaaaga gggtcataat ccataagaaa
     3061 attgcagtct atttgcggtg ggctgtctag tatttcaaat ttggcattag aagacggaga
     3121 acttttgacc tgtgtaacta aactgccatt ctcattttgg aatgaatctc tgtaagtttc
     3181 cagcaacctg tagccaaccg atataccggc tattataagc attacaaagc ctgcaaccaa
     3241 tgcaatccgt aaacgactgc ggttaggttt gtcttttgcc gggacggaat cattttttat
     3301 gctaagtaat attttacgct ttagctcatt acttgccttc aaatcattta atccggaaaa
     3361 caattcactc ttcatcataa aaaactcctt tcaatttggt cttgagcttt tctctgccgc
     3421 gtttgagccg cgttctgatt gtggactctt tttcagatag cagaccggct atctctgagg
     3481 atgaatatcc ttcataatag taaaggtaga ttacatctct gtatttttga ggcaggttca
     3541 tgacctgttt tattacttcc cgcttgttgt catcgggtat gggcaaatac atttcatgct
     3601 gcagtgaaac ctttttactc cacatgcttt taagtatatc tttgcagtag ttggaagtaa
     3661 cagtaatcaa ccaggctttg atatgatctt cgttgttaaa ctgcggagtt atccgaaaaa
     3721 gttttaagaa tatttcctgg gttgcatctt cggcatccga atgatttcta agatacataa
     3781 aagccagtct gaacaccata tccgtatatt tgtcaagtat ggccaaaata aagtcatcag
     3841 tgtgacttaa tccatatgtc attataaacc ccctttcata aagtacacga tttataacgg
     3901 gaaaatgttt cataatcggt aaaataatct taacattttt taaacattgt attaaggtaa
     3961 cttttgcaaa taatactaat gtggtgtaat attaatttag caatcagatg ctatacatct
     4021 catgcttttg tttatacaat cacatttgta atataaacaa tataaaatat cgggaggaag
     4081 tatgggtata aagaagttat atcagaaaag gattgaggag tttagcggta ttgtagacag
     4141 gcttgacaag gcctcgggtc tttatagcat attgcgtctt gtaacctttg cggtgggcac
     4201 actgctgaca atacttgcct atatatatat tggcagtatt tatggattta ttgttatggt
     4261 gctgtcggtt ggtatctttg tttttttgtg gtaaagcatc agaaaataat tgacagggca
     4321 gcaagatata aaagaatggt tgagattaac aaaaggtgta tcgatagaat ggaaggaagc
     4381 tggacggatt ttgaagataa gggcttggag ttcgccgaca gtgagcatat atatgccggt
     4441 gatttggata tatttggaca tgcatcgctt tttcaatgga taaatacggc acatactttt
     4501 ttcggacgga tagccttgaa gaatctgttt acatcacctt caaaagatat agagaaaata
     4561 aggaaaaggc agaaggctgt aaaagaactt tccgataaaa ttgatttttg ccagaacttg
     4621 caatgtgagg ggatgggtaa tatcaaggct tcagaaaatc ccgataagct tgtaagctat
     4681 tcggaggaca ataaaaagct gttttccgaa aaacatatat cacagttgtt ttttattttg
     4741 cctgaattga cgataatctt tgtgatcctg tgcaggcttg atgcttctgt ttcaatatat
     4801 atacctcttt cattactgac tctacaggca gttatgagcc ttatattcta tgggagggtg
     4861 agtgctgttt taagaccggt aaacaaatac aaggatagga taaacgttta ccgcaagatg
     4921 atcgaaatga ttgaaaagga agattttgcg gatgagtacc ttaatgaatt aaagtcacgg
     4981 ttttttttgg gaaaagtatc cgcatcagcc caaataaagg gattggagag aattgtggat
     5041 gcagtggact tgggaaatgg aactattgtt gcggttatct taaatttgtt gctgttctgg
     5101 aatgttcatt gtgtgtttgc cctggaaaaa tggaaggaaa agtccggtaa atcaataaag
     5161 ggctggcttg agactatcgg tgaatttgag gctctggtaa gtctttctct ggtaacacaa
     5221 atgaatcctg aatgggtttt tccggttatc tcaaaaaaca aagtttcttt tagtgctgaa
     5281 aaaatgggac atcccctgat aaatgagggt aatcgtgtat gcaacagcat taatatggat
     5341 aacaggattt gcatagttac agggtcgaat atgtccggta aaacaactct tttgaggacg
     5401 gtgggtatta atctcgtatt agcctatgcc ggtacgtcgg tgtgtgcaaa agagtttatg
     5461 tgttccgtga tggatatttg tacttcaatg aggataaccg acgatttaaa cagcggtata
     5521 tctacttttt atgcagagct tttgcgtatc aaaaggatta ttgaccactc caggaaaaaa
     5581 agtccgatga tatttcttat tgatgaggtc tttaggggaa ccaattccct tgacagggtt
     5641 acgggagcaa agaatgttgt attgaatctg gacagggatt ggattgttgg aatgatctca
     5701 acccacgatt atgagctgtg caagctgggg caaaaagacg gcagcaggat tataaactgc
     5761 cattttactg aaacctacga gaataatcag ataaaatttg actatatttt aaggcctggc
     5821 cgcagcaaaa ctacaaacgc aaagtatctt atgaagatgg tgggaataga gcttctcgag
     5881 caatgaaaag aatttatatt attatattaa tgaatacttt gaagggataa agatgtttga
     5941 tggaaaagaa aaaagaaata atggaagaag acctatatgc tcccgtgaag gattatctta
     6001 ccaaacaggg ttatgatgta aaaggtgagg tacaaaactg tgatgtatct gcactaaagg
     6061 gagaaacact tcttgtggtt gaaatgaaaa aaacgttgaa tcttgatgtc atattgcagg
     6121 caaccctcag gcaaaggatt gcggatattg tctatatagc ggttcctaaa aagagcaggg
     6181 ttttgtttac aaaacggtgg aaggatatct gtcacctttt g
//
LOCUS       DAOOJC010000096         6195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_10376_length_6195_cov_3.938599, whole genome shotgun sequence.
ACCESSION   DAOOJC010000096 DAOOJC010000000
VERSION     DAOOJC010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10376_length_6195_cov_3.938599"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2351)
                     /locus_tag="PLA01_03965"
     CDS             complement(<1..2351)
                     /locus_tag="PLA01_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPD00489.1"
                     /translation="MSNYFYPEERIAIVGMGVLMPDAENVNLFWNNILNKKVSIKAVP
                     DRIMNKRLYYDPKVLGRANKNDKSYTEVAAILDVEDYTSLSSKFKIPPAVAEHMDPNQ
                     HMVIYCVDQALQSLASGNINKERTAVIMGTGAPGHQYNNVVRRNFFVKLKDYLKNKSG
                     LDSFLKPSQMDKFFKELSEFALKDSIPITEDTAPGILQNIIAARITNLFGISGPSYTV
                     DAACASALAASINGVMGLLRREYDAVITGGADVTLDDGSFTVFSAINALSPDGSYPFD
                     ARANGFVMGLGGTALVLKRLEDAIRDGDRIYALISGYGQGSDGKGKHIAAPSENGQCR
                     VIKKAFEMAQYGADTVEYIEAHGTGTPVGDVAEINALKKAFKEMGVGKREYCGISSVK
                     SNIGHLRYAAGGAGLVKAAMALYERVLPPTAGIETVNPKLELEGSPFYILTDRKQWKE
                     STSHPRRANVSAFGFGGADYHIALEEFRPEFVKKIYAFPKDENTFAQSRIPCGNNSCT
                     EPKLPYLNNSCTEPKLPYVNNSLTRSEKESCPATCTTESEREAYPVSCPYEDCSLEVV
                     LFSGNSLDEINEGYLRFIKTLKEYSDFSQTVLFNNLWASPKKRMRLAICVRTVNELEE
                     RWNLFNKLVQQSGLINTQGLNIKGIYFGEGDALTSDKAAWMFPGQASQYPNMLKEIYE
                     GYPQIESLYTQVNALWKARYGNEIMPLIFGDDEEHLKKELINTRNTHPAVFLSDIAMF
                     KLLTESGVKADYMIGHSLGEIAALYAGGMLDLKSAVALAGERG"
     gene            complement(2494..3474)
                     /locus_tag="PLA01_03970"
     CDS             complement(2494..3474)
                     /locus_tag="PLA01_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPD00490.1"
                     /translation="MNEACSKFVDELAIKRILSEKLQKVTHRSKPCAFHGLWKTFDGL
                     EGCKLDFSHVKIVDNGEHAEHFEFESFEKKQNYIENNTVYLYYHPSKNALGNILFLHG
                     LFDDKMLNYAFLIRMLNELGLNVYFMVLPYHYVRKPKSSAFSGEFFVSADLERSHNAF
                     KQSVFDVEASLQLIKHYSRLPCLLVGYSMGGCIAFRYHILRGKPEGTFIINPVTDLLS
                     LIWENPLMATIKRDLEICGYDKNNVYFVFKELDPCENLNSDFSTGNIGVVYSVYDQII
                     GEEKNRIFIERVAKAGLQNILEYHSGHLNILRVPKLSKDIFDFFAKCNKC"
     gene            complement(3492..4982)
                     /locus_tag="PLA01_03975"
     CDS             complement(3492..4982)
                     /locus_tag="PLA01_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPD00491.1"
                     /translation="MNYCDLLFQTENKDLDREIIIDAENGKRINFGGLEEQVLKVAGF
                     LKAKGYKPGTVISTHIYNSAETVIAFLAIQYIGCVVCLIDPLFTAEEVPYYIQDSCSK
                     GIFTYLEKDQLEIVVKSGVEVINADEIEKICQATVPLSYKKLEMYSFKKDELAMLLYT
                     SGSTSTPKGVMLTTGCFYTFLDKNHKSMYRYEPEDRLLCFVPFSHGFGSISLLIPALA
                     YKAAIVFLKTFHPVKVVDTIIKEKITHIFGVPTHYQQLLRYNAINLIRGKLKAAFCSA
                     APLNCDVVYSWYEKTGVYLDEGYGMSESTTLISTRMSRLPEESGNVGYPPDGIIELQV
                     VDEKDRPVEDEVIGELRLRGEGLMLGYFNRPKETEERLKNGWLYTGDLGYRKGDGSFV
                     VCGRKTEFINVAGLKISPVEVETAINSHTDVIDSVAVGVFDETYGEIVKAFVIKKEES
                     ALTERELIKYLSKKLAKFKVPKYISYVAEFPRNNIGKIDRKALKNM"
     gene            complement(5078..6118)
                     /locus_tag="PLA01_03980"
     CDS             complement(5078..6118)
                     /locus_tag="PLA01_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HPD00492.1"
                     /translation="MNTEIRYPELKPLNLKRNSRIVSIGVALPDRVVTNQDIIDAYNL
                     IATDRAVQYSLGIKERRWAYVDQRLEDLMAKAARQCLDRAGIDIDRVDRVIYSRLLGD
                     YQIPASSINALRGLGARAGIPAFDISSACSGFLHAMDIAIRFIDSGDDYVLVIGGGIA
                     SRGIQILKKPDPSTVFLLGDAVVAMLIGYTEVRHFLSSYIMTNHFLYNTTYIPFGTSL
                     LKNDPAELDFDVFNMSVPDGKLILKGAVEHSRIVAEKLLSDTGLSLDEIDFFITSDQS
                     TKIWEAQLKALGVPFEKSLSLFYKYGNTVAAMSPLILDELISSGRLERGDLVMMMAHG
                     AGSSSAGMIFRY"
BASE COUNT         1803 a         1473 c         1038 g         1881 t
ORIGIN      
        1 atacccctct cgcctgccaa agccaccgca gattttaaat ccagcatacc accggcataa
       61 agagctgcaa tctcaccaag gctatggcct atcatatagt ctgcttttac cccggactcc
      121 gtcaaaagct taaacatggc aatatcactt aagaatacgg ccggatgtgt atttcttgtg
      181 ttaataagct ccttcttcag gtgctcttca tcatccccga aaatcagcgg cattatttca
      241 ttgccgtatc tggctttcca tagtgcatta acctgcgtat aaagagattc tatctgggga
      301 taaccctcat atatttcctt aagcatgttc gggtactgcg aagcctgacc cggaaacatc
      361 catgctgcct tatccgaagt cagtgcatct ccctccccaa agtatatacc ttttatgtta
      421 agcccttggg tgtttataag gccgctttgt tgtaccaact tattaaatag attccaccgc
      481 tcctccaatt catttacggt gcgaacacat atggcaagcc gcattctttt cttcggcgaa
      541 gcccaaaggt tgttaaacaa taccgtctgt gaaaagtcgg aatattcttt taaagtttta
      601 ataaacctta aatatccttc attgatctca tctaaagaat tacccgagaa aagtacaact
      661 tccagtgaac agtcttcata agggcaacta accggatacg cttctctctc gctttcggtg
      721 gtacaggtgg caggacacga ttctttctcg cttcttgtga gagagttgtt tacataaggt
      781 agctttggtt ctgtacatga attattcaga taaggtaatt tgggttccgt gcatgaatta
      841 ttcccacagg gtatccttga ctgtgcaaaa gtgttctcat ccttcggaaa ggcatatatc
      901 ttttttacaa actccggtct aaactcctca agggcaatat gataatccgc tcctccaaat
      961 ccaaaagcac ttacatttgc tcttctggga tgagatgtgc tctctttcca ttgttttcta
     1021 tccgtcagta tgtaaaaggg ggaaccctcc aactccagct tcgggttgac ggtctcaata
     1081 ccggcagtcg gcggtagaac cctttcgtac aatgccatag ctgcctttac caatcccgct
     1141 cctcccgctg catacctcaa atgaccgata ttcgacttta cagaacttat tccacagtat
     1201 tcccttttcc cgactcccat ctctttaaat gccttcttta atgcatttat ttcagcaacg
     1261 tcccccaccg gtgttccggt cccatgggct tctatgtatt ctacagtgtc tgcaccgtat
     1321 tgtgccattt caaaagcctt tttaattaca cggcactgtc cgttttcact gggtgcggct
     1381 atatgctttc ctttaccgtc gcttccctga ccgtatccgg atataagtgc atatattctg
     1441 tcaccgtccc ttattgcatc ctccagcctc ttcaaaacca gtgccgtccc tccaagcccc
     1501 attacaaaac cattggctcg agcatcgaaa ggataagatc catccgggga taaagcattt
     1561 atagcagaaa aaactgtaaa ggatccatca tccagagtga catccgcacc accggtaatc
     1621 actgcatcat attcccttct taaaagtccc ataacgccat ttatagaagc ggcaagggca
     1681 gatgcacatg cagcgtctac agtatatgac gggcctgaaa tcccaaagag gttggtaatt
     1741 ctggcagcta ttatgttttg gagtatgccc ggagcagtat cttcggtaat gggaatcgag
     1801 tcttttagtg caaattcgga aagctcctta aaaaacttat ccatttgtga aggtttgaga
     1861 aaagaatcca atccggattt gttcttcaaa taatctttca gcttaacaaa aaagttcctc
     1921 cggaccacat tgttgtattg gtgtccggga gcaccggtac ccattataac cgctgtacgt
     1981 tccttgttta tattaccgct tgccagcgat tgcaaagcct gatcaacaca gtatatcacc
     2041 atatgctggt tgggatccat atgctcggct actgccggag gaattttaaa tttgctgctc
     2101 aaggatgtgt aatcctcaac atcaagaatt gcagcaacct ctgtatagga tttgtcgttc
     2161 ttattggccc ttccaagaac cttcggatcg taatacagtc ttttattcat aatacgatcc
     2221 ggtactgctt ttatggaaac ttttttatta agaatattgt tccaaaataa gtttacattt
     2281 tccgcatcgg gcattaatac tcccattcca actattgcta tacgttcttc gggataaaag
     2341 tagttgctca tagtataacc tcctttttat agatttttga tttaagccca aaacacaacg
     2401 aagtacatca aacctattac aaggaccctt atgaccataa cctttccttt tattaaatca
     2461 aaacccaaag aattattagc gaatctgaca tagttaacat ttattacact tagcaaaaaa
     2521 atcaaaaata tcttttgaaa gtttaggtac tcttaaaatg tttaaatgtc ccgaatgata
     2581 ttccaatatg ttttgtagcc ctgcttttgc aactctttca ataaatattc tgttcttttc
     2641 ttctccaata atctgatcgt aaaccgagta tacaactcca atattgccgg tactaaaatc
     2701 cgaattcaaa ttttcacacg gatcgagttc cttaaaaaca aagtatacat tatttttatc
     2761 atagccgcaa atctcaagat ctctttttat tgtagccatc aaaggattct cccatatcag
     2821 cgacaataag tcggtaaccg gattaattat aaacgtccct tcaggctttc ctcttaaaat
     2881 atggtaccgg aaagctatac atcctcccat gctatatcct acaagcaaac acggcagccg
     2941 gctataatgc tttattagct gaagggatgc ttcgacatcg aaaactgact gtttaaaagc
     3001 gttatgtgat ctttcaagat cggcacttac aaaaaactcc ccgctaaaag cactgctttt
     3061 gggttttctt acataatgat agggaagtac catgaagtaa acatttagcc ccaattcatt
     3121 caacatcctg attagaaatg catagtttag cattttgtca tcgaacaatc cgtgcaaaaa
     3181 cagaatattg cccagtgcat ttttagaagg gtgataatat aggtatactg tattgttttc
     3241 gatataattc tgtttttttt caaaagactc aaattcgaaa tgctccgcat gttcaccgtt
     3301 atcaacaatt tttacatggg aaaaatcaag cttgcacccc tcaagtccgt caaaggtttt
     3361 ccaaagacca tgaaaagcac aaggcttgga gcggtgagtt actttttgaa gtttttcact
     3421 caaaatcctt tttatcgcca attcatccac gaacttcgaa catgcctcat tcataataac
     3481 accctctgtt gttacatatt ttttaatgct tttctatcta ttttaccaat attatttcgt
     3541 ggaaactcag caacataaga aatatatttg ggcaccttaa attttgcaag ctttttggat
     3601 aaatatttga taagttcccg ttccgttaat gctgactctt ccttttttat gacaaacgcc
     3661 tttacaatct ctccataagt ctcgtcaaaa actccaacag caacagagtc aataacatcc
     3721 gtgtgtgaat tgattgcggt ctcaacctca acaggagaaa tcttgagacc ggcaacatta
     3781 atgaattctg tttttcttcc gcagaccaca aaggaaccat ccccttttct gtatcccaga
     3841 tctcccgtgt agagccaacc gtttttcagt cgttcttcgg tttccttcgg acggttaaag
     3901 tatcccagca tcaacccctc tcctctcagt ctcaattccc cgattacctc atcttcaacc
     3961 ggtctgtcct tttcgtcaac aacctgaagt tcaatgatgc cgtcaggcgg atatcccaca
     4021 tttcccgatt cttccggaag tctggacatc cttgtggata taagtgtggt cgattcactc
     4081 atgccatatc cctcatcaag gtataccccg gttttttcgt accacgaata aacaacatca
     4141 cagtttaaag gtgctgccga acagaatgct gccttgagtt ttccgcgaat tagattgatg
     4201 gcattgtatc ttaaaagctg ctggtaatgg gtgggtacac cgaaaatatg ggtaattttc
     4261 tcttttatta tagtatcaac gacttttaca gggtgaaagg ttttcaagaa aacgatggcc
     4321 gccttgtagg ccaacgcggg aattaacaaa gaaatcgaac cgaatccgtg ggaaaaggga
     4381 acgaaacaaa gcagccggtc ctccggctca tacctataca ttgacttatg gtttttatca
     4441 agaaatgtgt aaaaacaacc ggtggtgagc attaccccct tgggagtaga agtggaaccc
     4501 gatgtatata aaagcatggc aagttcgtct tttttaaagc tatacatttc aagtttctta
     4561 taagataacg gaacagtcgc ctgacatatt ttttctatct catctgcatt tattacttca
     4621 acccctgact ttacgacaat ctccaattga tccttttcaa gatatgtaaa tatgcctttg
     4681 gaacaggaat cctgtatgta atagggtact tcctccgcag taaaaagagg atctataaga
     4741 cagacaacac aacctatgta ctgtattgcc aaaaaggcta taacagtctc agcactgttg
     4801 tatatatgcg ttgaaataac agtaccgggt ttatatccct tggctttaag aaaaccggca
     4861 acctttagta cttgttcctc aagacctcca aagttaattc tttttccgtt ttcggcatca
     4921 atgattattt ccctgtcaag gtctttgttt tcggtctgaa ataaaagatc acaataattc
     4981 attttattcc ctccccccaa tatccaataa gccatgatct ataaacaata aaacaagcct
     5041 aaatttgtaa cccggtttat aaatgcatgt tttaccttca gtacctgaaa atcatacccg
     5101 cactgctaga cccggcacca tgggccatca tcataaccag atcccctctt tctaacctac
     5161 cggaagaaat aagctcatcc aatatcaaag gactcattgc cgctacggta ttcccatatt
     5221 tataaaagag actgagactt ttttcaaagg gtacacccaa tgctttcagt tgagcttccc
     5281 atatttttgt agattgatcc gaggttataa aaaaatcaat ttcatccagg gaaagtcccg
     5341 tatcggataa aagtttttct gctactatcc gtgaatgttc cacagcacct tttaaaatca
     5401 acttcccgtc gggaacactc atattgaata catcaaaatc aagttcagca ggatcatttt
     5461 ttaacaagct tgtcccaaag ggaatatacg tagtattata caaaaaatga tttgtcataa
     5521 tataggagga taaaaaatgt ctgacctcgg tataaccaat aagcatggca accactgcat
     5581 cccccaacaa aaacaccgta ctggggtcgg gttttttcaa tatctgaata cctcttgaag
     5641 ctattcctcc cccaataaca agaacataat catcacctga atcaatgaac cgtatagcta
     5701 tgtccatggc gtgcaaaaaa ccactgcatg cagaagaaat atcaaaggcc ggtatccctg
     5761 ctcttgcccc caagcctctt aaggcgttga tagacgaagc cggaatctga tagtccccca
     5821 gaagccttga atatatgacc ctgtcaaccc tgtcaatatc aatacctgcc ctgtccaaac
     5881 attgcctggc agccttggcc atcaaatcct ccagtctctg gtcaacataa gcccatcttc
     5941 tttccttaat ccccaatgaa tactgaacag ccctgtctgt cgctattaga ttataagcat
     6001 caataatatc ctgatttgtc acaaccctat cgggaagtgc tacccctata gaaactatcc
     6061 ttgaatttct cttcaaattt aacggcttta actctggata tctaatctct gtattcataa
     6121 cctcacccta accataagta ctttatagta cttccaaatc aattaatctg aaaatataca
     6181 actaaatcta ttgtg
//
LOCUS       DAOOJC010000097         6100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_10548_length_6100_cov_3.840199, whole genome shotgun sequence.
ACCESSION   DAOOJC010000097 DAOOJC010000000
VERSION     DAOOJC010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10548_length_6100_cov_3.840199"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1844
                     /locus_tag="PLA01_03985"
     CDS             <1..1844
                     /locus_tag="PLA01_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HPD00493.1"
                     /translation="MSEDEWNSLVNNDGDNEAIQEFESKIEKISTIIEYLDRYDTRKK
                     GLFSSKRDISINDYSLVLKNQDKLLSVVEEINQYDRTMSDLKSEKNKYSNLILSLKPW
                     VFLDIPLDLTSTKASTVLIGVVPEIADANKMKQEIADEVPECHFEVIGEDKDQSYLLI
                     IYLTSKEEAVTKILKQYGFSKVTFKDLKGTAKHNISEASKRIDSIDKDIEDIQKKISD
                     YVIYKNDLEVLYDHMVIERDRKKILNNLVKTKKIFMLEGWLPEKLSEEVKSYIEKCCD
                     CYVDIIKPADDEEFPVLLANRDIGRSVESITNMYSVPNSREIDPNTVMAPFFILFFGL
                     MLSDGGYGALLAIGSGIILKMFKLDESMKRFMKLMLYCGISTMLWGFLFGGWFGIPNI
                     PALWFNPVDDPEMLLSFSLLFGVIHIFVALGMKAANLIREKKYLDVVFDVVFWYITFV
                     GFILFLLPYVPKINTDEVSDLVNAGKYILLIGVVLLVLTQGRNSKNIFAKLIGGVGSL
                     YDLIGFMSDVLSYSRLLALGLATSVIGSIVNQMALMLGFNNIFKIILIVAVLLAGHLI
                     NFAINALGAYVHSSRLQYIEFFGKFYKGGGTSFEPFKANTKYINLKH"
     gene            1857..2321
                     /locus_tag="PLA01_03990"
     CDS             1857..2321
                     /locus_tag="PLA01_03990"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF005124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HPD00494.1"
                     /translation="MNFFLNYGGSVLAVLGAAIGFALAGIGSSKGVGTAGEAGAGVLT
                     EEPGRFGAVMVLEALPSTQAIYAFVIAFLTLQKVELTMPLQNGLSLFVAALPVGVVGL
                     ISGIWQGKVATAGIHMVAKRPDGMGRGIVMALMVEMFAILGFIISILMLGKV"
     gene            2377..2973
                     /locus_tag="PLA01_03995"
     CDS             2377..2973
                     /locus_tag="PLA01_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HPD00495.1"
                     /translation="MAGVDKIKERILEEARSQAESNIKRAEEEASNIIASAKKEADAK
                     RAVILEKAKKEAQEVKKRLKSVAELEARKRKLQAKQELVEEAFKKTLEKLNSLSDKEY
                     EQIISQMLINSVESGSEEIILSPRDKKRLSPNFVEEINKKLVAKGIKGSLSLSKESRD
                     INGGFVLKSGDIETNSSFEALIRAKREEIEPEVIKVMF"
     gene            3011..4030
                     /locus_tag="PLA01_04000"
     CDS             3011..4030
                     /locus_tag="PLA01_04000"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HPD00496.1"
                     /translation="MTKQKKIKELDFNYAVARIRAIEKGLLDKTKFDRMIEAKTSDEA
                     LKVLLEAGYGRAGTELKSVFEYEKLLKDESKKVYELLNELAPGQDVINMFLLSNDYHN
                     VKVILKAEFSGQTETSIFIEPGFVPVEKLKLMIKDRNLKELPEIMKKAIEECIDTYNR
                     TGDPQLIDLILDKAQYSHMLNISGSFCNKFINELVLSLVDLTNIKIFLRVKNLNKTWD
                     FLQKILIPGGSIDIKVFFENMDAPLENFVQAIKYSRYGSLLENSIESINRTGSLSGFE
                     KLSDNFITSLVQKARYITFGIEPLIGYLMAKETEIKNARIIMVGKINNISNDIIRERL
                     REAYV"
     gene            4023..4343
                     /locus_tag="PLA01_04005"
     CDS             4023..4343
                     /locus_tag="PLA01_04005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HPD00497.1"
                     /translation="MYKIGVIGDKDSILGFKAIGMSVFPVTASNEAEEILDKIAREQY
                     AVIYITEQFAKDIVSAIDKYSDDRFPAIIPIPSNQGSLGIGMMGVKKNVERAVGADIL
                     FRDE"
     gene            4380..>6100
                     /locus_tag="PLA01_04010"
     CDS             4380..>6100
                     /locus_tag="PLA01_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPD00498.1"
                     /translation="MSQGTIVKVSGPLVIAEGMRDANMFDVVRVSEHRLIGEIIEMHG
                     DKASIQVYEETAGLGPGEPVVSAGAPLSVELGPGLIETIFDGIQRPLEAMRKMVGSNI
                     TRGIDVASLDRNKKWKFEPTVKKGAKVSSGDIIGKVQETSIVEHRIMVPYGVSGTVEE
                     IFDGSFTVEETVAKVRNDKNELVEIKMMQKWPVRVGRPYKEKLAPDSPLVTGQRVIDT
                     LFPLAKGGVAAVPGPFGSGKTVVQHQLAKWADAEIVIYIGCGERGNEMTDVLREFPEL
                     KDPRTGESLMKRTVLIANTSDMPVAAREASIYTGMTIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGRVVCLGSDGREGALTAIGAVSP
                     PGGDISEPVTQATLRIVKVFWGLDASLAYRRHFPAINWLTSYSLYLDRLDKWINENVA
                     RDWDSLRADTMKILQEESELEEIVRLVGVDALSPGDRLTLESAKSIREDYLHQNAFHD
                     VDTYTSLNKQYRMLKLILGFYYKGKRAIEAGVSIKDLFDLSVRERIGRAKYTPENQVD
                     SSFNEIE"
BASE COUNT         1931 a         1085 c         1444 g         1640 t
ORIGIN      
        1 acatgtctga agacgaatgg aacagcttgg ttaacaacga tggggacaat gaagcgatac
       61 aggaatttga atctaagatc gaaaaaattt ccaccatcat tgaatattta gaccgatatg
      121 atacccgaaa aaaggggtta ttctcttcaa aacgtgacat tagtataaat gactacagtc
      181 ttgttttgaa aaatcaggac aagcttttgt ccgtagtcga agagattaat caatatgacc
      241 ggaccatgtc cgatctgaag tcggaaaaaa acaaatattc aaacctgatt ctaagcctca
      301 aaccatgggt atttttggat attccactcg atttaacttc aacaaaggct tctacagttt
      361 taatcggcgt tgttcctgaa atagctgatg caaataaaat gaagcaggaa atagccgatg
      421 aagtacctga atgccatttt gaggtaatag gagaggacaa ggaccagagt tatcttctta
      481 ttatttattt gacatcaaaa gaagaggctg taaccaaaat attaaaacaa tacggtttct
      541 ccaaagtaac cttcaaggat ctaaaaggga cagccaaaca caatatcagt gaagcatcaa
      601 aaagaataga cagcattgat aaggatattg aggacattca aaaaaagatc tccgattatg
      661 taatctacaa gaatgatctg gaggttctat atgaccacat ggttattgag cgggacagaa
      721 aaaagattct taataacctg gtcaagacaa agaagatatt tatgctggaa ggatggcttc
      781 ctgagaaatt gtccgaggaa gtaaaaagct atatagaaaa atgctgtgat tgttatgtgg
      841 acattataaa accggctgat gacgaagagt ttcctgttct tcttgccaat cgcgatatcg
      901 gaagatcagt tgaatccata accaatatgt acagcgttcc aaactccaga gagattgacc
      961 ctaatacggt aatggctccg tttttcatat tgttcttcgg gcttatgctg agcgatggcg
     1021 gctacggtgc cttattggct ataggttccg gtattatact aaagatgttt aaacttgacg
     1081 aaagtatgaa gaggtttatg aagctcatgc tttactgcgg tatttccaca atgctgtggg
     1141 gttttctttt cggaggatgg ttcggtatac ccaacatacc ggcgttatgg ttcaatcctg
     1201 tggacgatcc ggaaatgctt ctgagctttt cattactctt tggtgtaatc catatatttg
     1261 ttgctctcgg aatgaaagct gccaacctta ttagggagaa aaaatatctg gacgtagtat
     1321 tcgatgtggt tttctggtac attacatttg taggatttat tctctttttg ctgccctacg
     1381 ttccaaaaat taataccgat gaggtgtcgg atcttgttaa tgccggaaag tacattcttc
     1441 ttatcggtgt agtgcttctg gttcttactc agggccgcaa cagcaaaaac attttcgcaa
     1501 aactgattgg cggagtaggt agcctttatg acctgatcgg attcatgagt gatgtattgt
     1561 catactcaag acttttagcc cttgggctgg caacctctgt tataggctct atagtcaatc
     1621 aaatggcact tatgcttgga tttaacaaca tatttaaaat aattttaatc gttgcagtat
     1681 tgttggcagg ccatttaatc aattttgcca tcaatgctct tggagcatat gttcactcca
     1741 gcaggctcca gtatattgaa tttttcggca agttctataa gggtggaggt acatcctttg
     1801 aaccctttaa agcaaataca aagtatatta atttaaaaca ttaggaggta atagttatga
     1861 atttttttct caattacggt ggtagtgttt tagcggtttt aggtgcagca ataggttttg
     1921 cccttgcagg tataggttcg tcaaagggcg taggtacagc cggtgaagct ggagcaggag
     1981 ttttaacgga agaacccgga aggttcggag ctgttatggt acttgaggct cttcccagta
     2041 cccaggcaat atatgcattc gttatagcct ttttgacctt gcaaaaagta gaattgacca
     2101 tgcctcttca aaacggtctt tccctgtttg ttgccgctct tcccgtaggt gttgtcggcc
     2161 tgatttcagg aatatggcaa ggtaaggttg caaccgcagg aatacacatg gttgctaaaa
     2221 gacctgacgg tatgggtaga ggtatcgtaa tggcccttat ggttgaaatg tttgcaatac
     2281 tcggattcat aatttcaata ttaatgctgg gtaaggtttg atttatcccc ggtgtagata
     2341 gaaacttcaa attatataaa agggagtgtt ccccctatgg caggagtaga taaaataaaa
     2401 gaaaggatac ttgaagaagc acgctcacag gcagaatcaa acattaagcg tgctgaggaa
     2461 gaagcatcca atattattgc atcagctaaa aaggaggctg atgccaaaag agctgttatt
     2521 cttgaaaaag caaagaaaga agctcaggag gtaaaaaaaa ggcttaagtc ggtagctgaa
     2581 ctcgaagcaa gaaagagaaa gcttcaggca aagcaggaat tggtagaaga ggcctttaag
     2641 aaaactcttg aaaaacttaa cagcttatct gacaaagaat atgaacaaat aatatcacaa
     2701 atgcttatta attctgtaga aagcggctcc gaagagatta tactttcccc tcgggacaaa
     2761 aaaaggcttt caccgaattt tgttgaagaa atcaataaga aactggttgc aaaaggaatc
     2821 aagggaagtc tttccctatc aaaagagtcc agagacataa acggaggctt tgttttaaaa
     2881 tccggtgata tcgaaacaaa cagctccttt gaggcattaa taagggcaaa aagagaagaa
     2941 atagaacctg aagttataaa agtgatgttc tgataatact tattatttgc cggaaaagga
     3001 gggatggaat ttgacaaagc aaaagaaaat caaggaattg gatttcaact acgcagtagc
     3061 aaggattcgt gcaattgaaa aaggactttt ggataaaacc aagtttgaca gaatgatcga
     3121 agcaaagaca tcggatgaag ctttaaaggt tcttttggaa gcgggctatg gtcgggcagg
     3181 cacggaatta aaaagcgtat tcgagtatga aaagctactg aaggatgaaa gcaaaaaggt
     3241 gtatgagctg ttaaacgagc tggctcccgg tcaagatgta ataaacatgt ttttgctgtc
     3301 caatgattat cataatgtaa aagttatctt aaaggctgaa ttttcaggac agacggaaac
     3361 ttcaatattt attgagcctg gttttgttcc tgttgaaaag cttaagctta tgataaagga
     3421 cagaaatttg aaagaattgc ctgaaattat gaaaaaggct attgaagaat gcattgacac
     3481 ctataacaga accggtgacc ctcagttaat agaccttatt ctggacaaag cccaatacag
     3541 tcatatgctt aacatatcgg gttctttttg caacaaattt ataaatgaat tggttttgag
     3601 ccttgttgac cttaccaaca taaagatttt tctcagggta aaaaacctta acaagacatg
     3661 ggattttctt caaaagattc ttatcccggg aggcagtatt gatataaagg tgttttttga
     3721 aaacatggat gcacctctcg aaaactttgt tcaagccata aagtattccc ggtatggctc
     3781 gttgcttgag aacagcatcg aaagcataaa tcgtaccgga agtctttcag gctttgaaaa
     3841 gctgtcggat aattttataa cctctttagt tcaaaaggca agatatataa ctttcggtat
     3901 cgagcctttg ataggatatc tcatggcaaa ggaaactgag attaaaaacg ccagaatcat
     3961 tatggtgggc aaaatcaata atatttctaa tgatatcatt agagaaaggc tgcgagaggc
     4021 ttatgtataa aataggagta attggagaca aggacagtat tttaggattt aaagcaatag
     4081 gtatgtcggt atttcccgtt actgcttcca acgaagctga agaaatattg gataaaatag
     4141 cccgggagca atatgcggta atatacatca cagagcagtt tgccaaggat atagtatctg
     4201 cgattgacaa atacagtgat gacagatttc ccgcaattat acccattccc agtaaccaag
     4261 gttctttagg catcggtatg atgggagtga aaaagaatgt ggaacgggcc gtgggagcag
     4321 acatactttt cagggatgaa tagcgtaagg ttaatatttt ttgaaggaag ggatgaagcg
     4381 tgagccaagg aacaatagtt aaggtttccg gtcctttggt aattgccgaa ggtatgcggg
     4441 atgcgaacat gtttgacgtt gtacgtgtaa gcgaacaccg cctgattggc gaaataatag
     4501 aaatgcatgg agacaaagct tctattcagg tttacgagga aacagccggc ttgggtcccg
     4561 gagaaccggt ggtttccgcc ggtgcaccct taagcgttga gctaggaccc ggacttattg
     4621 agactatctt tgacggtatt caaagacctc ttgaggctat gaggaaaatg gtgggaagca
     4681 atattacaag gggtattgat gttgcctccc tggaccggaa taagaagtgg aaatttgagc
     4741 ctacggtaaa aaaaggagca aaggttagtt ccggtgatat aatcggtaag gttcaggaaa
     4801 cctctatagt agaacataga ataatggttc cttacggagt cagcggtacc gtagaagaaa
     4861 tattcgatgg tagtttcacc gtcgaagaaa ctgttgcaaa ggttcgcaac gacaaaaacg
     4921 aactggtgga aataaaaatg atgcaaaaat ggccggtgcg tgtaggaagg ccctacaaag
     4981 aaaagctcgc cccggactca cctttggtta caggccagag ggttattgac actctcttcc
     5041 cccttgccaa gggtggagtt gctgctgtac cgggaccttt tggaagcgga aaaacagtgg
     5101 ttcagcatca gcttgcaaag tgggcagatg ctgaaatagt tatatatatc ggatgcggcg
     5161 aacgtggaaa cgagatgacc gacgttttga gggaattccc agagcttaaa gacccaagaa
     5221 ccggggagtc tcttatgaaa agaaccgttt tgatagcgaa tacatccgac atgcccgttg
     5281 cagcacgtga agcctccata tatacgggta tgaccatagc ggaatatttc agggatatgg
     5341 gctacagtgt ggctctgatg gcggactcca cctcccgttg ggcagaagcc ctaagggaaa
     5401 tgtccggccg tttggaggaa atgcccggtg aagagggtta tcccgcttat ctcggttcaa
     5461 gacttgccca gttctacgaa agagcaggaa gggttgtatg ccttggaagc gacggcagag
     5521 aaggtgccct gacagcaatc ggtgccgtat cgcctcccgg aggtgatata tccgagccgg
     5581 ttacccaggc tacactgaga atcgtaaagg tattctgggg cttggatgcc agccttgcct
     5641 atcggcgtca tttccctgca ataaactggc tgacaagcta ttctctgtat cttgacaggc
     5701 tggacaaatg gattaatgaa aatgttgcaa gggactggga ctctcttcgg gcagatacaa
     5761 tgaagatcct tcaggaagaa tcggaactgg aggaaattgt gcgtctggta ggtgtcgatg
     5821 ccctttcccc aggcgaccgt ttgacactgg agtcagccaa atccatccgt gaggattacc
     5881 ttcaccaaaa cgcattccac gatgtggata cttatacctc gttgaacaag cagtacagaa
     5941 tgctgaagct tatactggga ttctattata agggcaaaag ggctattgaa gcgggagtaa
     6001 gcatcaaaga cctgtttgac ctttccgtca gggaaagaat aggaagggca aaatataccc
     6061 ctgaaaatca ggttgacagc tcctttaatg aaattgaaaa
//
LOCUS       DAOOJC010000098         6019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_10712_length_6019_cov_3.362508, whole genome shotgun sequence.
ACCESSION   DAOOJC010000098 DAOOJC010000000
VERSION     DAOOJC010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10712_length_6019_cov_3.362508"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLA01_04015"
     CDS             <1..343
                     /locus_tag="PLA01_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPD00499.1"
                     /translation="AKKDIVVGHKAFGYLCKAYGLRQNAVEGLNSEFEPTPAKMAEIV
                     KFIRDNDIKVIFTDGMSNPKVANTVAGEVGARTLVLNTLGGLTDEDIKSGKDYFSVMR
                     ENLEALKKALE"
     gene            378..1157
                     /locus_tag="PLA01_04020"
     CDS             378..1157
                     /locus_tag="PLA01_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HPD00500.1"
                     /translation="MEKVIEVKDLCFGYGERLILENLNFSVKKGDFVGIIGPNGSGKS
                     TFVKLLLNNIKPLRGEIKILGQSIEKFNCWNKIGYVSQKANSFNTGFPATVEEVVRAN
                     LFSKVGLFKRVKNEHKEMVYDALKTVGMEGYKDKLIGNLSGGQQQRVFIARVLVSRPE
                     IMFLDEPTVGIDAQTEGALYCLLGKLNSEMGITIVMISHDIGAITVHANKIACMSNKK
                     IIINENSREFADSSLRDVYGYDVNLHAHRHLCENCCMKEGK"
     gene            1157..2020
                     /locus_tag="PLA01_04025"
     CDS             1157..2020
                     /locus_tag="PLA01_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPD00501.1"
                     /translation="MFGIFGEDFVLNLEFMQRAYLIGILIAVMAPAIGVIIVLRRLSM
                     IGDSLSHTSLAGVALGLISGFNPIIVATVFSVAAALGIEKIRRAFPKYSEISIAVILS
                     AGIGLAGVFSGFVKNGFSLTSYLFGSIVAISDFEFGLIITLGIVVIFTVILMFKELFF
                     ITFDEEAARLAGVPVKMINFIFMVLTAVTIAVSSKVVGVLVISSLLVLPSASAIQIAK
                     SFKQTMVYSVLYGMLSIVSGLTLSFYLNLVPGGTIVLISVVLLVLTLMYKNLVKGILL
                     KRVLNQEKTRV"
     gene            2157..4052
                     /gene="htpG"
                     /locus_tag="PLA01_04030"
     CDS             2157..4052
                     /gene="htpG"
                     /locus_tag="PLA01_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPD00502.1"
                     /translation="MNYENGTISINTENIFPIIKKWLYSEKDIFIRELVSNGSDAISK
                     LKKLAAIGEAEVGSDEKYYIKVVLDKEKKTIQIIDNGIGMTADEVKKYINQIAFSGVK
                     DFVEKYKDKTDEAQIIGHFGLGFYSAFMVSSKVQIDSLSYMKDAEAVRWVSTGGIEYE
                     MDRSDRNERGTTVTLYIAEDSEEFLENYKLREVLVKYFSFLPYEVFFEDANTVREDKD
                     KDKESKEPEEPKPINDTNPLWLKNPKDCTEEEYKDFYGKVFFDFNEPLFWIHLNMDYP
                     FNLKGILYFPKLRNEMDSFEGQIKLFYNQVFVADNIKEVIPEFLMLLKGTIDCPDLPL
                     NVSRSFLQNDGYVNKISNHITKKVADKLTSLFKDERENFNKYWDDINPFVKFGCIKEK
                     KFYERVKDIIIFKTIDGEYVTLKDYLEKNKEKHENKVFYVSDEKQQAQYIKMFRDNDL
                     NAVVLSSPIDNHFIQFMEMNESGVKFLRIDSDLSDSMKDNTGADENSLKEITENLEKV
                     FKENLGNDKLKIKVEALKVTTVPGMILLPEQTRRIQEMSRMYGGFNFGGLEGEEETLV
                     LNSNNGLIKTILDIKDKDDRKDDLKLICEHIYDLSTMSHKQLEPEAMTKFIERSNLML
                     SRFALAN"
     gene            complement(4296..5960)
                     /locus_tag="PLA01_04035"
     CDS             complement(4296..5960)
                     /locus_tag="PLA01_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPD00503.1"
                     /translation="MLEKYLSKVNFDSYEDFVENYNLIVPENFNFAYDVVDKIAEENP
                     NKTAMVWCDENGDEATFTFGQLKEYSNKAANFFKRIGVKKGDSVMLILKRRYEFWFAI
                     LALHKIGAVAIPATHLLTAKDIIYRNNAADIKMIVSVAEDEVIKHIEGSLEKSPTIKA
                     KALINSEKEGWFNFNSEIDKESSQFERPTGEDAPANDSMMLLYFTSGTTGMPKMVAHD
                     FTYPLGHIHTAKYWQNVIEDGLHLTVADTGWAKAAWGKIYGQWLSGSAVFVYDYEKFV
                     PHELLDVICKYGVTSFCAPPTIFRFFIKEDLTKYDFSKLKHCAVAGEPLNPEVYNQFY
                     KATGLKLMEGYGQTELTVTLGTFPWIEPKPGSMGKPAPGYDIDLLDENGNSCEVGEEG
                     QIVIRTDKRKPVGMFCGYYRDAELTNKVWHDGIYYTGDMAWRDEDGYYWFVGRADDVI
                     KSSGYRIGPFEVESALLEHPAVLECAITAVPDPIRGQVVKATVVLTKNYTPSDDLVKE
                     LQNHVKTVTAPYKYPRIIEFVKELPKTISGKIRRVEIRDKDKADKE"
BASE COUNT         1789 a         1066 c         1372 g         1792 t
ORIGIN      
        1 tgcgaaaaag gatattgtgg tgggacataa ggctttcgga tatctttgca aggcttatgg
       61 actcaggcag aatgctgttg aaggcttgaa ttccgagttt gagcctacac cggctaaaat
      121 ggccgaaata gtgaaattta taagggataa tgatataaag gtgatcttca ccgacggtat
      181 gtcaaacccc aaggtggcaa acaccgttgc cggagaggtg ggagcccgga ccttggtttt
      241 gaatacgttg ggagggctta ccgatgagga tattaaatcc gggaaagatt atttttcagt
      301 aatgagggaa aaccttgagg ccttgaaaaa ggctcttgag tgagtttgtc aaattccttg
      361 ctaaaggcgg atatcttatg gaaaaggtta ttgaagtaaa ggatttatgc tttggatatg
      421 gagagcggct catcctggaa aacctcaatt tttcagtgaa gaagggtgat tttgtaggta
      481 taatcggtcc caacggttca gggaaaagca cctttgttaa gctgctatta aataatatta
      541 agccccttcg gggtgaaata aaaatattgg gtcagagcat tgaaaagttt aactgctgga
      601 acaaaatagg gtatgtatcc cagaaggcaa attccttcaa tacaggtttt cctgcaacag
      661 tagaagaggt tgtgcgggct aatttgtttt caaaggtggg acttttcaag agggtaaaga
      721 atgagcacaa agagatggtt tatgatgcac ttaagactgt tggaatggaa gggtataagg
      781 acaagttgat cggcaattta tcgggaggcc agcagcaaag ggtgtttata gccagagtat
      841 tggtttcaag acctgaaata atgtttttgg atgagcctac tgtcggaata gatgctcaga
      901 cagaaggggc tctgtactgc cttttgggaa aactcaacag tgaaatggga ataacaatag
      961 ttatgataag ccatgatatt ggggcaatta ctgtgcatgc aaacaagatt gcctgtatga
     1021 gcaataaaaa aatcatcatc aatgaaaact caagggaatt tgcagacagc agccttagag
     1081 atgtatacgg ttatgatgta aatctgcatg cccacaggca tttatgtgaa aactgctgca
     1141 tgaaagaggg gaaataatgt tcgggatttt tggagaggat tttgttttaa acctggagtt
     1201 tatgcaaagg gcgtatctga ttggaatact tatagctgtt atggctccgg ccataggagt
     1261 tataatagtt ttgagaaggt tgtcgatgat aggggattcc ctttcccata cttctcttgc
     1321 cggtgtggcg ttaggcctta tttcgggctt taatcctata atagtagcaa cggttttttc
     1381 cgtggcagca gccttgggga tagaaaagat caggagggct tttccgaaat attcggagat
     1441 ttccattgcg gtaatactct ctgccggaat tggccttgcg ggtgtgtttt ccggttttgt
     1501 aaaaaacggt ttctcgttga catcctattt attcggaagc attgttgcaa taagcgattt
     1561 cgaattcgga ttgataatca ccctgggtat cgttgttatt ttcactgtta ttcttatgtt
     1621 caaggagctt ttctttatca cgtttgacga ggaggccgca agacttgccg gtgttccggt
     1681 caaaatgata aattttattt ttatggttct gactgcagtg accattgccg tatcgtcgaa
     1741 ggtggtgggg gtgctggtta tctcttcgct gctggttctt ccttccgcat cggccataca
     1801 aattgcaaag agctttaaac agaccatggt ttattctgta ttatacggaa tgctttcaat
     1861 agtatcggga cttacccttt ctttttacct gaacctggtt cccgggggca caatagtgct
     1921 gatcagcgtt gttcttttgg ttctgaccct gatgtataag aatcttgtta aaggcatttt
     1981 gttaaaaagg gtattaaacc aagaaaaaac gagggtttag aattatgaac aaggttttgt
     2041 tagcactcgc cgttgctgag tgctaaattc tccttgacaa gcttttgttc acaattataa
     2101 tatatgtata agttaagatt atattagaat taacggttta tataggaggg actagaatga
     2161 attacgaaaa tggaactata tccattaaca cagagaacat attccctatt ataaagaaat
     2221 ggctgtactc ggaaaaggat atttttatcc gggagctggt ttcaaatggc agtgatgcga
     2281 taagcaagtt aaaaaagctt gcagccattg gtgaagcgga ggttggcagt gacgaaaaat
     2341 attatattaa ggttgtgctg gacaaagaaa aaaagaccat acaaataata gacaacggta
     2401 ttggcatgac tgccgatgaa gtaaagaaat acataaacca gattgctttt tcgggggtaa
     2461 aggattttgt tgagaagtat aaggacaaga ccgatgaggc tcagataata gggcactttg
     2521 gactgggttt ttattctgcc tttatggttt caagcaaggt gcagattgac agtctttcct
     2581 acatgaagga tgccgaggct gtcaggtggg taagtacagg cggtattgag tacgagatgg
     2641 acaggtccga cagaaatgaa aggggaacca ctgtaaccct gtatatcgct gaagacagcg
     2701 aggagttcct tgaaaattat aaattgcggg aggttttggt taagtatttt tcattccttc
     2761 cctatgaagt gttttttgaa gacgcaaata cagtgaggga agataaggat aaggacaagg
     2821 aaagtaagga gcctgaggaa ccgaaaccaa taaacgatac aaatcctctg tggctcaaaa
     2881 atccgaagga ttgtaccgaa gaagaataca aggatttcta tggaaaggtg tttttcgatt
     2941 ttaatgagcc tttattctgg attcatctta acatggatta tccttttaat cttaagggaa
     3001 tactgtattt tccgaagctt agaaacgaga tggatagctt tgaagggcag attaagctgt
     3061 tttataacca ggtatttgtt gcagacaata tcaaggaggt tattcccgag tttttgatgc
     3121 tcttgaaggg taccattgac tgcccggatc ttccgcttaa tgtttcgaga agctttttgc
     3181 agaatgacgg ttatgtaaac aagatttcca accatataac caagaaggtg gcagacaagc
     3241 tgacttcatt attcaaggat gaaagggaaa acttcaacaa atactgggac gatataaatc
     3301 cctttgtaaa attcggatgc ataaaggaaa agaagtttta tgaaagggtt aaggacataa
     3361 ttatattcaa gactatagac ggagaatatg tgacattgaa ggattacctt gaaaagaata
     3421 aagaaaagca tgagaacaag gtgttctatg tatcggatga gaaacagcag gcccagtata
     3481 taaagatgtt cagagacaat gacttaaatg ctgttgtgct gtcatcaccg atagacaatc
     3541 attttattca gtttatggag atgaacgaaa gcggtgtcaa gttcctgaga atagattccg
     3601 atttgagcga cagcatgaag gacaataccg gggctgacga gaattcttta aaagagatta
     3661 cagagaattt ggaaaaggtc tttaaggaaa acctcggaaa tgacaagctg aaaatcaagg
     3721 tggaggcttt gaaggtaaca acagttcccg gaatgatcct gttgccggaa cagacaagaa
     3781 ggattcagga gatgagcagg atgtacggcg ggttcaattt cggcggattg gaaggggagg
     3841 aagaaaccct tgtcctaaac agcaacaacg gtttgattaa gaccattctt gatattaagg
     3901 acaaggatga taggaaagat gatttaaagc ttatctgtga acatatatac gacctttcaa
     3961 cgatgagtca taagcagctt gagcctgagg ccatgacgaa gttcattgag aggagtaatt
     4021 taatgctttc aaggtttgcc ttggcaaatt aagcaaaggg tggggtattg taaaatccaa
     4081 gcgaacaaaa gctccggggg catcgtgaag tgctcccgga gctttttggt cattttactt
     4141 tgtgaaatcc cgctaaagaa gtcctgcata gttatatagc ataactttgc aggacttctt
     4201 ttgtttccga tgtactaaac tattaggtga agaaaaatat gaaagaggct tgatttgcgt
     4261 aactcaagtt tcagaaatat ttttcttcac tttttttatt ctttatctgc tttatcctta
     4321 tctcttattt caactctcct tatttttccg ctgatggttt tgggaagctc ctttacaaat
     4381 tcaattattc taggatactt gtatggagcg gtaaccgttt taacatggtt ttgaagctct
     4441 tttacaagat catcgctggg tgtatagttt ttcgtaagca caaccgtggc tttaaccact
     4501 tgacccctga tcgggtccgg aacagcagta attgcacatt cgagtaccgc agggtgttct
     4561 aaaagtgcac tctctacctc aaagggtccg atacggtatc ccgagctttt tataacatcg
     4621 tcggcccttc cgacaaacca gtaataaccg tcttcatccc gccatgccat atcaccggta
     4681 tagtatattc cgtcatgcca taccttgttt gtcagttcag catcacggta atatccgcaa
     4741 aacattccca caggttttct cttatcggtc ctgataacta tctggccttc ttcaccgacc
     4801 tcacaggaat taccgttttc atccaataaa tcaatgtcat aaccgggagc aggcttgccc
     4861 atggaaccgg gcttcggttc tatccagggg aatgtaccca gggtgacggt tagctctgtc
     4921 tgaccatatc cctccatcag ctttaaacct gttgctttat aaaactgatt gtatacttca
     4981 ggattcaatg gttctcctgc aacagcacag tgcttaagtt tgctgaaatc atattttgtg
     5041 aggtcttctt ttataaagaa cctgaaaatt gtaggcggtg cacagaagga agtcactccg
     5101 tatttgcata taacatctaa aagttcgtgg ggaacaaatt tctcatagtc atatacaaaa
     5161 acagcactgc ccgaaagcca ctgaccgtat attttccccc atgcagcttt tgcccatcca
     5221 gtatcggcga cggttaaatg aagtccgtct tcaattacgt tttgccagta tttggcggtg
     5281 tgaatatgcc ccaaaggata tgtgaaatca tgggctacca ttttgggcat gccagtagta
     5341 ccggaggtaa aatagaggag catcatgctg tcattggcag gtgcatcctc accggtgggt
     5401 ctttcaaatt gggaagattc tttgtctatt tcactgttaa agttaaacca accttctttt
     5461 tcactattta tgagtgcttt agcttttatg gttggagact tttccagaga accttcaata
     5521 tgcttgatta cttcatcttc agccacgctg actatcattt ttatatctgc tgcattgttt
     5581 ctataaatta tatccttggc tgtcaaaaga tgagtagcag gtatggcaac agcacctatt
     5641 ttatggagtg caagaatagc aaaccagaac tcataacgcc tttttaaaat aagcattact
     5701 gaatcgcctt tttttactcc aatccttttg aaaaagttgg cagccttgtt actgtattct
     5761 ttaagctgac caaaagtaaa tgtagcctcg tccccgttct catcacacca caccattgct
     5821 gtcttattgg gattttcttc ggcaattttg tcgacaacat catacgcaaa attaaaattt
     5881 tccgggacaa ttaaattata gttttcaaca aagtcctcat aagaatcgaa attaactttt
     5941 gatagatatt tctctaacat tacactaaga cctccattaa ataattacag ccaaaaattt
     6001 ggcgactttt ccgtttaac
//
LOCUS       DAOOJC010000099         5974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_10816_length_5974_cov_3.571887, whole genome shotgun sequence.
ACCESSION   DAOOJC010000099 DAOOJC010000000
VERSION     DAOOJC010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10816_length_5974_cov_3.571887"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..308
                     /gene="hfq"
                     /locus_tag="PLA01_04040"
     CDS             63..308
                     /gene="hfq"
                     /locus_tag="PLA01_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HPD00504.1"
                     /translation="MVKNNINLQDVFLNQVRKEHIPVTIYLTNGFQLKGMVKGFDNFT
                     VVLDSEGRQQLIYKHAISTISPMKLVNLIFNDNRGEQ"
     gene            complement(506..1147)
                     /gene="lexA"
                     /locus_tag="PLA01_04045"
     CDS             complement(506..1147)
                     /gene="lexA"
                     /locus_tag="PLA01_04045"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPD00505.1"
                     /translation="MQNKVNEKQQKILDFLNEQIEKNGYPPSVREICSAVGFKSTSTV
                     HGYLEKLRKEGLINKAPSKPRALRVVDSNKNSKQAKTKEFYSGKELVEVPIVGKVTAG
                     QPILAVENIEDTFPLPLDFVQNSSAFMLRVRGDSMIEAGIFDNDYILIKQQSTANNGD
                     IVVALIEDEATVKTFYKENGYVRLQPENKYYEPIIVKDNLSILGKVIGVFRKM"
     gene            1331..1615
                     /locus_tag="PLA01_04050"
     CDS             1331..1615
                     /locus_tag="PLA01_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPD00506.1"
                     /translation="MKKRYVLKNKKRFFAFIVLMLVCVCTVINSTRTYGYKEPEYKTI
                     FVRRGDTLWGIAQRYNKRGDIRKYIYDVMKLNNLESCDIIEGMELKVIIE"
     gene            complement(1654..2466)
                     /locus_tag="PLA01_04055"
     CDS             complement(1654..2466)
                     /locus_tag="PLA01_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00507.1"
                     /translation="MNNDFEKQIENQLFAELGAGKIKTYSEMQKRKAELMNQNPSKRS
                     NIRLTTDKKLLFGILLLACTFLLVVYVIDPIAKLRLPVTQKVYNNHSATDDPVQTDTS
                     EKVFIIRNGSDIWYITPMADYKIAAKVKGKHRIFPFGDAVEAISKYDLALAWGQLIDP
                     KYDKYIKYSQSGRGYRFSCSANCPLSVSYITTHSANTHIIAANKQILKGLKYIKTNDI
                     IYMEGLLVNAEMYKENNSYTWETSLDRNDNSFGSGCEIMYVKRLEIGNKVYQ"
     gene            complement(2928..3785)
                     /locus_tag="PLA01_04060"
     CDS             complement(2928..3785)
                     /locus_tag="PLA01_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017353471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPD00508.1"
                     /translation="MEWPNRMRDAIDYIENNIENELDPEEIAKVACCSTFHFQRMFLM
                     LTGVSLKDYIRKRRLTLAAQEISVSDTKVIDLALKYGYDSPESFSKAFRRTHGCTPSA
                     ARKSGVKLKAFPRISFHLYLKGDKDMDYRIVERKEFKVIGKALTVSTKNGENFKRIPM
                     FWEECSKDGTCDKLCSLSNSDNLLGICIDFDHEKEEITYMIAVEDKGYSSDGFVTRVI
                     PEATWAVFSVVGPMPDAIQEVWKKIFQEWFPSTGYEHAQGPEMEVYLDGDADSDDYRS
                     EIWVPIIKK"
     gene            3971..4324
                     /locus_tag="PLA01_04065"
     CDS             3971..4324
                     /locus_tag="PLA01_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HPD00509.1"
                     /translation="MDKLYEDTLLLDFYGQLLTERQFEIMDMHFNNDYSLGEIAEQLN
                     ISRQGVYDNIKRGKALLKRMEEKLGLVEKFVKQKNKAQEILINIKSLSKNGICEEDII
                     TLKRIEEGIIQIIEG"
     gene            4429..5769
                     /gene="ffh"
                     /locus_tag="PLA01_04070"
     CDS             4429..5769
                     /gene="ffh"
                     /locus_tag="PLA01_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPD00510.1"
                     /translation="MVFEGLSNKLQEALKKLKGKGRVTEKDVKEMMREIKLALLEADV
                     NFKVVKDFISKVSERAVGQDVLESLTPGQQVIKIVHEELINLMGSSNSKITFASKPPT
                     VYMMVGLQGSGKTTTSGKLANLLRKQGKNPLLVACDVYRPAAIKQLQVVGGQLGVEVF
                     SMGDKTNPVDISKAAVEYAKSKQHDIVIIDTAGRLHINEELMEELQNIKTAVKPHEIL
                     LVVDSMTGQDAVNVAETFNDKLGVDGIILTKLDGDTRGGSALSVKAVTGKPIKFAGMG
                     EKLSDLEPFFPDRMASRILGMGDVLSLIEKAQEAFDEKKALELEKKMRTQQFTLDDFL
                     DQMQQIKKMGPLNQIMGMIPGLNANALKNMDFDEKKMSHVEAIIKSMTKKERNDPSII
                     NGSRRKRIAAGSGTTVQEVNRLLKDFEEMKKMMKMMTDMGKRGKKGFNKLRLPF"
     gene            5803..>5974
                     /gene="rpsP"
                     /locus_tag="PLA01_04075"
     CDS             5803..>5974
                     /gene="rpsP"
                     /locus_tag="PLA01_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPD00511.1"
                     /translation="MAVKIRLKRMGAKKKPFYRVVVADSRFPRDGRFIEEIGTYNPLT
                     NPSEINIDTEKAQ"
BASE COUNT         1875 a         1061 c         1224 g         1814 t
ORIGIN      
        1 cgtaattaaa tgtttgttaa aaaaataaag aagaaaaaga gagaaataga ggaggattat
       61 ctgtggttaa aaacaatatt aatttacaag acgttttttt gaatcaagta agaaaagaac
      121 atatcccggt tactatttat cttaccaacg gatttcaatt aaagggaatg gtaaagggtt
      181 tcgataactt taccgtagtt ttggatagcg aaggaagaca gcagcttatt tacaagcatg
      241 caatttcaac cataagccct atgaaactcg taaaccttat tttcaatgat aacagaggcg
      301 aacaataatc gctaaataaa gaaatcgata tcaggaagta tacttttata aagggtttta
      361 cttctttcaa atggcggctg agaaatttta agaggcttat gcaaagcata agcctcttat
      421 tagtggtatt tcacgaagtg aaatagcaaa aagcgaaagg gctcgatgcc cttgagcttt
      481 tgtagagtgg tatttcacac caaccttaca tttttctgaa tactcctatt acttttccca
      541 ggatggacag attgtctttt actataatgg gttcatagta tttattttcc ggctgcaatc
      601 tgacataacc gttttcttta taaaacgtct taaccgttgc ttcgtcttca atcagtgcta
      661 caacaatatc tccattgttt gctgtagact gttgctttat gagaatataa tcgttgtcaa
      721 agataccggc ttctatcata ctgtctcccc tgacccgaag catgaaggcg gaagagttct
      781 gaacaaagtc taaaggcagc ggaaaagtat cctcaatatt ttctaccgct aaaatgggct
      841 ggcctgccgt aaccttaccc acaataggaa cctccaccag ttcctttccc gaatagaatt
      901 cttttgtttt ggcctgtttg ctgtttttat tgctgtcaac aaccctgagg gccctcggtt
      961 ttgaaggagc cttgtttatc aagccttcct tccgcagctt ttctaaatag ccatgaaccg
     1021 ttgaggtgga cttgaatcca acagcactgc aaatctccct taccgaagga gggtagccgt
     1081 ttttttcaat ctgctcgttt aaaaaatcca gtatcttttg ttgcttttca tttactttat
     1141 tctgcatgtt tttcaccccg gaaaagtatt attctatatg ttacataaaa aaacacatac
     1201 atttattcat gttaaaagta ttataacatt attaaataaa aaaatcaaac ttacgttcgt
     1261 ttttttattg acaaagaaca tacgttcttg tataatactc atatagaaca ttagttcgag
     1321 ggggatttat atgaaaaaac gttatgttct caaaaataag aaacggtttt ttgcgtttat
     1381 tgttttaatg ctggtatgtg tatgcactgt tatcaattct acccgtactt acggatacaa
     1441 agagcctgag tataaaacca tatttgtaag aagaggggat actttatggg gaattgcaca
     1501 gaggtataac aagcggggag acattagaaa gtacatatat gatgttatga aattaaacaa
     1561 tcttgaaagc tgcgatataa ttgagggtat ggagcttaaa gtgattattg agtaatattt
     1621 ctatagccgt tttacagtgc aggcagggta atgttattga tatactttgt ttccgatttc
     1681 cagccttttt acatacatta tttcgcaccc gctgccaaaa gaattgtcgt ttcggtccaa
     1741 gctggtttcc caggtgtaag aattgttttc tttatacatt tccgcattca ccagaagccc
     1801 ttccatatag attatgtcat ttgtttttat gtacttcaac ccttttaaga tttgcttgtt
     1861 tgcagcaatt atgtgagtat ttgcagaatg ggtggttata taactcacac ttaagggaca
     1921 gttagcggag caggaaaagc ggtaccctct tcccgattgg gagtatttaa tatacttgtc
     1981 atacttcggg tcaatgagct ggccccatgc caaagccaga tcatatttgc ttattgcttc
     2041 tacagcatcc ccaaaaggaa atatacggtg tttacccttg acttttgcag caattttata
     2101 atcagccatt ggcgtaatat accaaatatc cgaaccattt cttataataa agactttttc
     2161 tgatgtgtca gtttgaacag ggtcatctgt tgcactatgg ttgttataga ccttttgggt
     2221 taccggtaat ctaagttttg caataggatc tattacataa accacaagca gaaaagtaca
     2281 agctaataac agaataccga ataatagttt cttatcggtt gttaaacgaa tattagacct
     2341 tttacttggg ttttgattca tcagttcggc ctttcgcttt tgcatttcac tgtaagtttt
     2401 tatttttcct gcacccagct cagcgaacaa ttggttttct atttgctttt caaaatcatt
     2461 attcaagaaa tcacctcatg gataggatat catataaaaa cccacaccat ctattataca
     2521 agaataattt ggatttttca cccgaaaatt tgcaattggg tatggtataa gcatgaatcc
     2581 atctgctgcc aatacgcttt ttgtctcaac tataatttat tgaaaaaaat attaaggata
     2641 ttcttcatcc agaatggtaa tatgcatttt ccggactgca ttacaaaacc aatgcagtcc
     2701 gggaggtgat aatggtaata attaacgtat tgaaatatgt gtaatttaat aacaggctaa
     2761 aaccagctta cttttttttc gtttaaaata aactaagaag aaactctgtt aaagcttata
     2821 cagggcttct ttttagtagg atttcacgaa gcgaaatgaa caaaaagcga tgggtgatcg
     2881 atgccccatc gcttttactt ctgaatttcg aattctcatg aattaaacta tttttttata
     2941 atcgggaccc atatttcgct gcgatagtca tcggaatcgg catcaccgtc aagatatact
     3001 tccatttcgg gaccctgtgc gtgttcgtag cctgtggacg ggaaccactc ctggaatatt
     3061 tttttccaca cttcctgtat tgcatcgggc attggaccta caactgaaaa cacagcccag
     3121 gtagcttccg ggatgactct ggtgacaaat ccgtccgatg aataaccttt gtcttccaca
     3181 gctatcatat acgtaatttc ttccttttcg tgatcaaaat caatgcatat acccagcaga
     3241 ttatcactgt ttgataagga acacagctta tcacatgtac cgtctttcga gcactcttcc
     3301 cagaacatcg gtattctttt gaagttttct ccattctttg ttgacactgt caacgccttg
     3361 ccaataacct tgaactcttt tctttccaca atcctgtaat ccatatcctt atctcctttc
     3421 aagtataagt ggaaggaaat gcgtgggaac gccttgagtt ttacaccgga tttgcgtgca
     3481 gcagaaggtg tgcacccatg ggttcttcga aatgctttgg aaaaggactc cggggagtca
     3541 taaccgtatt tgagggccag atcgataacc tttgtatcgg aaacagatat ctcctgggct
     3601 gccaatgtga gtcttcgttt tctgatgtaa tctttaagcg acacaccggt cagcattaaa
     3661 aacattctct gaaagtgaaa ggttgagcaa catgctacct ttgcaatttc ttccgggtcc
     3721 agttcgtttt caatattgtt ttcgatatag tcgattgcat ccctcattct gttaggccat
     3781 tccaaagcac tcatctcctt taatcatata gtatcacgga taaggatata atacctgtca
     3841 ttccttgctt tttaatgaca gatttatttt atcaattcta aaaatatttt tcaaaataac
     3901 ttgactaaaa caaaattatg acttaaaatt gggtgtaaag tatttttgct ttacaggtga
     3961 gaataatatt atggataagt tatatgaaga tactctttta ctggactttt acggacaact
     4021 gcttacagaa aggcagtttg agataatgga tatgcatttt aacaacgatt attcgttggg
     4081 cgaaattgca gagcagctga atattagccg gcagggtgtg tatgataata taaagagggg
     4141 caaagcactg ttaaaacgta tggaagaaaa gcttgggctt gtggagaagt ttgtaaagca
     4201 aaaaaacaaa gctcaggaaa tacttattaa tatcaaaagc ttgagcaaaa acggtatatg
     4261 tgaagaagat attataaccc taaagaggat tgaggaagga ataatacaga taatagaagg
     4321 ctgagtatat attatagtat ggctaaaagg catgctttac atgcctgagg ttggatgggc
     4381 aagaagttag tatacgataa ccttaataga tctgaggtgt cttaaaatat ggtgtttgaa
     4441 ggattatcga ataagcttca ggaagcgttg aagaagctta aaggtaaggg aagagtaact
     4501 gaaaaagatg ttaaagagat gatgcgggag ataaagcttg cacttttgga ggcggatgtt
     4561 aacttcaaag tggttaaaga ctttatttca aaggtgtctg aaagggctgt aggacaagat
     4621 gttttagaaa gcctgacacc ggggcagcag gtaataaaga tagtgcatga agagctcatt
     4681 aatctcatgg gctcgagcaa cagcaagatt acctttgctt caaagccgcc taccgtttat
     4741 atgatggttg ggcttcaggg ttccggtaag accacaacat caggaaaact ggccaatttg
     4801 ttaagaaaac agggtaagaa tcctctttta gttgcctgtg acgtttacag accggcggcg
     4861 ataaagcagc tccaagtagt gggaggacag ctcggtgttg aagtgttctc aatgggagac
     4921 aagacaaatc ccgttgacat atctaaagca gctgtagagt atgcaaagtc aaaacagcat
     4981 gacattgtta taatagatac ggcaggtcgt ctccatataa atgaagagct tatggaagag
     5041 cttcaaaaca taaagacagc tgtaaagcct catgaaattt tacttgttgt ggattccatg
     5101 accgggcagg atgccgtaaa tgtggcggaa acctttaacg ataagttggg tgtcgacggc
     5161 ataatcctca caaagcttga cggagatacc cgtggaggtt cggccctttc ggtaaaagcc
     5221 gttacgggaa aacccattaa gtttgccggt atgggtgaaa aactgagcga tcttgagccg
     5281 tttttccccg acaggatggc atcaagaatt ctgggtatgg gcgatgtgtt aagccttata
     5341 gagaaagccc aggaagcctt tgatgagaaa aaggcattgg aactggaaaa gaaaatgaga
     5401 acccagcaat tcacgctgga cgattttctt gaccagatgc agcagataaa gaagatggga
     5461 cctttaaacc agataatggg gatgataccc ggcttaaatg ccaatgccct taaaaatatg
     5521 gattttgatg aaaagaagat gtcccatgtt gaggctatta ttaagtccat gaccaagaag
     5581 gaaagaaatg atccttcaat aattaacgga agccgcagaa agagaattgc agccgggagc
     5641 ggtactaccg ttcaggaagt aaacaggctt ttaaaggatt ttgaagagat gaaaaagatg
     5701 atgaagatga tgactgatat gggtaaacgc ggcaagaaag ggtttaacaa gctaagattg
     5761 ccgttttaat aatgaattag catatggcag gaggtgataa aaatggcagt taagataaga
     5821 cttaagagaa tgggtgcaaa aaagaaacca ttttatagag ttgttgtagc agattctaga
     5881 tttcctcgtg acggtaggtt tattgaggag attggtactt ataatcctct cacaaacccc
     5941 agcgagatta acatcgatac agaaaaagct caga
//
LOCUS       DAOOJC010000100         5953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_10860_length_5953_cov_3.162767, whole genome shotgun sequence.
ACCESSION   DAOOJC010000100 DAOOJC010000000
VERSION     DAOOJC010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10860_length_5953_cov_3.162767"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLA01_04080"
     CDS             complement(<1..188)
                     /locus_tag="PLA01_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00512.1"
                     /translation="MSQMDYDRIQHKAGQAGMSMTAFITASALGKNITVVDGLDKVLA
                     ELKAIGKNLNQLTTLCNMG"
     gene            592..1542
                     /locus_tag="PLA01_04085"
     CDS             592..1542
                     /locus_tag="PLA01_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6076 domain-containing protein"
                     /protein_id="HPD00513.1"
                     /translation="MAYSFKVYISDGKEYFEYDNGQKTTKKSFVFSQSLMDLLYLDIW
                     FYGDLFEKMGKSVRSLYSSRDSQWSDEIKKGLDILARQHIYFEFLRLDWMDRLKKAEE
                     QEFQEIVDLLPYKKLTHIPSNIGTMQDQLLDLWRDVLDILSPDKPIQKKMVDYYNEKG
                     RDKLNTFQFQPQPMSFEVIDKKTFTEVLYPKDIYDIIDYFVRECIKREQRFRVCKNCG
                     KFFALTGHINTEYCDRPFDSEGRTCKEMGALRLWEKKKADTPALKAYSKEYKRRFAWI
                     KYGKISKEAFYEWSEEARKKRDLCVNGNITLEKFKAWLKD"
     gene            1694..2479
                     /locus_tag="PLA01_04090"
     CDS             1694..2479
                     /locus_tag="PLA01_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00514.1"
                     /translation="MIKKLIFISICLAGLSFLFNGCTANDKNNKEIQPIETKKLEAPI
                     TQNEFVDAFTFLNNELPEEQKFSSENVPKELYETGPVLSLYINGDTPYIVSWIDGGKI
                     LIFFKAENSKKTPLYMNIAPIKAVKLVGDQMENGIRLVEVTSYGASSSNQRENVKIFA
                     ITGNTVTTAWDYDTLKSYSHPVEGKNLFEFVIETSSFSYSPWYYTKPGYDEKDVMKII
                     VNEAKEVIDTPSEDLEKVLYSRKTTSQKIFEWDSKQMKFIEKR"
     gene            complement(2522..3262)
                     /locus_tag="PLA01_04095"
     CDS             complement(2522..3262)
                     /locus_tag="PLA01_04095"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HPD00515.1"
                     /translation="MGYTFKYASFPSDRKTAMTNAYNSIILPNEGYDVLSLTTFQDLS
                     NAYTSVKSNPSWSVVSDWTFTNFRTSTEIANEADVSVVARCLLGENSNVGSTAAERQK
                     AIRCSAKVIMNRAANGGYGGSDYRSVVLASGQFDAMRGQNTGATNPVFYVGAINYWAY
                     SMWVAHHMYSDMEPFNTDEIGSNYYFFYDTQAASGGSPGAFLKANGSPCTSVSDIISN
                     GVYYQNTHQQLTVKSAVASVIFFSYKTV"
     gene            complement(3973..5953)
                     /locus_tag="PLA01_04100"
                     /pseudo
     CDS             complement(3973..5953)
                     /locus_tag="PLA01_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518927.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT         1640 a         1221 c         1141 g         1951 t
ORIGIN      
        1 cccatattgc acagagtggt caactggttt aagtttttgc cgatggcttt tagttcagca
       61 agcaccttgt caagcccgtc caccactgtg atgtttttgc ctaaagccga tgcggtaata
      121 aaggctgtca tgctcatgcc cgcctgtccc gctttatgct ggatgcggtc ataatccatt
      181 tggctcatcc gcatggcaaa cattttttcc ttgctcatac gctcatcctt tcttttgctc
      241 ccctttaggg gctgcgggct tttgggggtt cgggggtatc ccccgaccag ctgtttgtat
      301 gggcggcggc gagagccagc cgtctataca aacaatatgc tggcctaccc tgaaagggta
      361 gttacgcgca cccccgacac ctgcgcagac gccccctttg cctgctgcag cagtcgcaat
      421 gattgcgctt gcaccgacct gctgatatac tgtaaacagt atagatatag actaatataa
      481 ttttaatatt ctatagtcta tattggtatt gtattccata cttgctttct tgtcaatact
      541 tatgtataat agatttaata ttagtcgata atctatgctg aggtggtaaa gatggcgtac
      601 agctttaaag tgtatatatc cgatggaaaa gaatattttg aatatgataa cgggcagaaa
      661 acaacaaaaa agagctttgt gttttcgcaa agcctgatgg atttgttgta tctggatatt
      721 tggttttacg gcgacttgtt tgaaaagatg gggaagtctg tccgcagtct gtattccagc
      781 agagacagcc aatggtctga tgaaataaaa aaaggtttgg acatcctcgc aaggcagcac
      841 atctattttg aattcctgcg cctggactgg atggacagac tgaaaaaggc ggaggaacaa
      901 gaatttcagg aaattgtaga tttgttgccc tacaagaagc ttacgcatat cccatccaac
      961 attggcacta tgcaggacca actcttggac ttatggcggg atgtgctgga catcctatcc
     1021 ccggataaac cgattcaaaa gaagatggtg gactattaca acgaaaaggg cagggacaaa
     1081 ctaaacacat ttcagttcca gccccaaccc atgagctttg aagtaattga taaaaagacc
     1141 tttaccgaag ttctgtatcc aaaggacatt tacgatatca ttgactactt cgtacgggaa
     1201 tgcatcaaac gggaacaacg cttccgtgtc tgtaaaaact gcgggaaatt ctttgccctg
     1261 accggacata tcaacacgga atactgcgac aggccttttg acagcgaagg gcgcacctgc
     1321 aaggaaatgg gggcactgcg cctatgggag aaaaagaaag ccgacactcc ggctttaaag
     1381 gcgtatagca aggaatacaa acggcgcttt gcatggatta agtatggcaa aatctcgaaa
     1441 gaagcttttt acgaatggtc agaagaagca aggaaaaaac gggatttatg tgtaaatggt
     1501 aatataacgt tggagaaatt taaggcatgg ctgaaggatt aagatttata tcattagtag
     1561 taagtaataa gtatgtttta ttcgtataaa cactaaaata taatacaggt aagctcgttt
     1621 atcagcggaa ctaaaaatgg gctattgtcg tctaatgtga tactaaaatc caatttatag
     1681 ggaggcaatc tttgtgatta aaaagctgat ttttatttct atatgtttgg ctggactatc
     1741 ttttttattt aacggctgca cagcaaatga taaaaataac aaagaaatcc aaccaattga
     1801 aactaaaaaa ttagaagctc ctattactca aaatgaattt gttgatgctt ttacttttct
     1861 taataatgaa ttgcccgaag aacagaaatt ttccagcgaa aatgtcccaa aagagctcta
     1921 cgaaacaggc cctgttcttt ctctttatat taatggtgat accccgtaca tagtgtcttg
     1981 gatagatggt ggtaagattt taattttttt caaagctgaa aactcaaaga aaacaccact
     2041 ctatatgaat atagcaccga taaaggcggt taagctagta ggagatcaga tggaaaatgg
     2101 tatacggctg gtagaagtaa catcatatgg tgccagcagt tctaaccaga gagagaacgt
     2161 aaagattttt gcaataaccg ggaataccgt aaccactgct tgggattatg atacattgaa
     2221 atcctattca caccctgtag aaggaaaaaa cctgtttgaa tttgtgattg aaaccagttc
     2281 cttcagttat tccccatggt attatactaa acctgggtac gatgaaaaag atgttatgaa
     2341 gataatagta aatgaagcca aagaagttat agatacgcct tctgaggatt tggaaaaggt
     2401 actttattcc agaaaaacaa cctctcaaaa aatatttgag tgggacagta aacaaatgaa
     2461 attcattgaa aaaaggtagc tcccgcgagc tacccttttt caatgaattg acctttttat
     2521 tctatacagt tttgtaactg aagaaaatga cactagcaac cgctgattta actgtaagct
     2581 gctggtgtgt gttttgataa tatacaccat ttgatataat atctgacaca ctggtgcacg
     2641 gggatccatt tgcctttaaa aatgcaccgg gggagccacc tgaggcagcc tgagtatcgt
     2701 agaagaagta ataatttgag ccgatttcat ctgtgttaaa cggttccatg tcgctgtaca
     2761 tatgatgagc aacccacatt gaataagccc aataattaat tgctccaaca taaaatacag
     2821 gattggttgc tcctgtattc tgacctctca tagcatcgaa ttggccagag gccagtacaa
     2881 cacttcgata gtcgcttcca ccatacccac catttgcagc tctgttcatg ataacttttg
     2941 ctgagcacct gatagccttt tgacgttctg ccgcagtact tcctacatta gagttttcgc
     3001 ccaaaaggca acgtgcaacg acagatacat cagcctcatt ggcaatttca gttgacgtcc
     3061 tgaaatttgt aaaagtccag tcagatacaa ctgaccaact cgggtttgat ttcacagaag
     3121 tataggcatt gctgaggtct tgaaaggttg ttaatgaaag aacatcataa ccctcgttag
     3181 gaagtatgat actgttgtat gcatttgtca ttgctgtttt tctatccgaa ggaaaggaag
     3241 catatttaaa agtataaccc ataaaaattc tcctctcata cttttgtttt tagattgttt
     3301 ttcatgcttt aggattttaa caggttattg caattatgat aaaaatagat attgcatcaa
     3361 ctgcaactat ggtatatcat attgagacag aggctcactg aatattcgtt tatttaaaaa
     3421 cttccgaggt tcgagatgag ggaatataaa ggagccttta atgttatttg gtgactttat
     3481 atatatcata gcatgttcac ctcctttcgt ttacaaggga atatagttaa ttgcgaaact
     3541 ctgaagtcct tgccaatgct gtatttttac tgctttatta taggattctc ctccgcaaaa
     3601 ctccaaagcc ctatattttt aagccatttg catgtggata actttactgg aaattgttta
     3661 taactttcgg ctttttatgt taggtttcaa aaatttgtaa ttctgtgtct cggaacccgc
     3721 attgtaccat taaaactttt tcaagttatc cacatttttc ggttttttag tttcgcaatt
     3781 acctaacaag ggaataatct tattcctttg ctatataaga caggagaaga gggaaaaatg
     3841 tttcataatt tcctaaattc ggttcatatt tcctactaag gtgggtaaaa agtctcatct
     3901 gttgtaggta taatttactt ttaactgaag gccacgttcc agaataagaa cgtggccttt
     3961 tataccgatt tgttactgtt tacaatgttt tttgtttgta cctcgtaaaa aacataatac
     4021 cggattgttg gcaacatagg tatataaatt gagtccgtca cctctgaagg tgtcttcctg
     4081 cataaacctt cctatgcttg ggttatagta tcttgctctt aaataatatt ggtcagtaac
     4141 tgaatcatgc agttctcccg agtacttgaa tctgttgtct actttttcaa cactgtccaa
     4201 tatattacca aaggcatcat agctgtagct gtttactact tctcctcttc cattgtaaga
     4261 gcggtaacat ctccgtgggc attgtggata taataatagg tatccttgtt actgtcctct
     4321 ttttccaata tcgtatgtcc ccgaactatt ctgtccttca atccccaatt ctcgtcaact
     4381 tcacatgcta tgtttcttcc attgtatatg tacctgttgt attttccgtt ttctatcgat
     4441 accgtcctta aattctcagc atcatatatg ttctccatat atgttccgtc aggattctgt
     4501 actcgtacca gtctattaaa tccgtcatag ctgtataatt tacttaattc gcctcctttc
     4561 tctcttatga ggtttcctct ttcatcatac tcatattccg ttgtcaaccc gttcttaatc
     4621 cgttgtaata atctattgca attatcatac tcatattgct ccaatatgtc tccaaattcc
     4681 ctcttaagtc tgttgccgtt ggcatcatag gtaaacctct cttttatcta gcctgggtac
     4741 attacctcct gtaagcggtt taaagcatcg tatctgtagg atgtgacatt ctcattttcc
     4801 tcttttagaa tctggttgcc gtttccgtca taggcatacc tgtacataat catctctttg
     4861 cccgacggat ccatatttga aagtcttgtt atcttcttat ccagattgta ttcatatttt
     4921 gcacttactc cgcttccata caatatccgg gctatggtgc tatctgtatt gtattcgtac
     4981 tttgcagaaa ttttatctcc attggatact gctgttaccc ttcccagcat gtcgtatgta
     5041 tattccactg gtgttccgct tacatcggtt agtctggata taagtccgtt tttattgtag
     5101 tcatattgca gtacctcttt gccattggtg gccttgctct tcagtcttcc tgccaaggtg
     5161 tatgtatacg tatgtgtcgt actgccgctt attgacgcag ccagacttcc gtcaatatta
     5221 tactcgtatt tttccaccaa tccttcttct tcccaacggc tggtaatatt attatctgcg
     5281 ttataggtgt aataggtact gtgtccgtct ttccctatcc tttgcactct tcttccctgc
     5341 ctgtcatatt tgaaggttat tttccttcct gccgggtcta ttatctcaga caggagattt
     5401 aagctgttgt atgtatacct ggtggtattt ccgttgccgt cggtggtgga tataaggttt
     5461 cctgcatagt cataatcaca ttttatgttg gtaccgtcag gctccgttac gtttattatt
     5521 cttccccaca gatccagagt gtaccttgta ctgtttccgt tgccgtcaac tattccggtt
     5581 atgttacctt ctgcatcata ggtgtactgc tggctctttc tgccttgaag ctttgcctcc
     5641 ggtgttgtta tttccagaag acgtcttgcc atgtcataac caaattccac tccattcctg
     5701 tcattgtcat tatcagccga tttatagccc attgagtcaa ttttcctgat tatttctccc
     5761 gctgtgttgt atatattcct ttctaccact atccccgctg catttgttat ctgcataagc
     5821 cggttcattg aatcataaag gtatgttgta cccaatccgt catcttcttc gggtacatag
     5881 ttttccggat ccacatattt tatgatattt cctgaagtgt catagaaaat cctggtggta
     5941 tttccgtcgg ggt
//
LOCUS       DAOOJC010000101         5909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_10958_length_5909_cov_3.833447, whole genome shotgun sequence.
ACCESSION   DAOOJC010000101 DAOOJC010000000
VERSION     DAOOJC010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10958_length_5909_cov_3.833447"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2069
                     /locus_tag="PLA01_04105"
     CDS             <1..2069
                     /locus_tag="PLA01_04105"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HPD00516.1"
                     /translation="YLKKHPPILTEILTEKLSERASDEEDKDTLQEKRDSKKWIFGKQ
                     RAFVLGASAIACVYALCITIPYMGLAAIGSNQPSMKAFANRDEAVNFFLRAKDYSSSY
                     SRELNKFSQFLYDFNKVFINIAIIANPNFSLELPSMGMKASSDFASGMGGQNTAGGTE
                     AVYSDGIYKGTSKTNVQVENVDEADIVKTDGEYVYYINGSNLFIIRSNPPEQMEIVYR
                     HNFLEGEPREMFLYNDYIGVIISEKRRTVVKTYNIKDRSNPVAERTLELDCGYLTSRM
                     INNHLYLVTTSYVRDIDYPEYSDTSANDSIVEMDYKDLFLMNNPSNDYVKMNVVAAFP
                     IDDAGSEAQVKAYIGGDGENVYVSTNYIYIADEIADTTVVNQPIRDFFDCLSTDDSLY
                     YEYQYATNIYRIEINDGTIGKFDSASVRGAVHNQFAMDEYNGYFRLTTQTGRWSSASS
                     NVFVLDENMKLCGSLKGLAPEEHIYASRFMGDRLYLVTFKQVDPLFVISLEDPRDPRV
                     LGKLKIPGYSEYIHPLDENHIIGFGKDAVDSVTGNFAWSQGVKIAIFDVTDVNNPKEK
                     FVEIIGDRGSDSPLLQNHKALMYMKNLELMAFPVSVVENYPVNDYYQYNVFQGAYVYN
                     VNSTEGFKLRGRITHLNQPLQDIWQSAQIDRIVYSDGVLYTLSGEKVKATKYTDMKDM
                     SEVPLR"
     gene            complement(2360..3445)
                     /locus_tag="PLA01_04110"
     CDS             complement(2360..3445)
                     /locus_tag="PLA01_04110"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HPD00517.1"
                     /translation="MKRLLFCFLFVCIAGSFFGFVEVREADSITSFLEKLFDLNGSKQ
                     VIVVSTNYEGDYKAAVDAYERKPEGWVKSFDTMKAVVGKNGFSTNKIEGDGKAPAGIF
                     RIGHAFGVPYGASITKLPYRKVTENDYWIDDITSAEYNTWVEYRGNPNDRWKSFERLN
                     ITPYKYSIVIEYNMNPVIAGKGSAIFLHIWKDSNSGTSGCIAVSEDNMLKLLKWLDPK
                     SNPVIIQGTGAMIKDMMNNADETVLYPVKVKVNGKEVDFDVYPRIVDGRTLVPVRAIF
                     EEMGAKVSWDEKEHTVTITRGSSKVVLKIGSKTAFIDNQEVELDVPASIINGRTLVPV
                     RFIAEGLRFNVGWDGGSRTVTIDGEEH"
     gene            complement(3488..4927)
                     /gene="miaB"
                     /locus_tag="PLA01_04115"
     CDS             complement(3488..4927)
                     /gene="miaB"
                     /locus_tag="PLA01_04115"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPD00518.1"
                     /translation="MDNNTKERKNIHVSTEEMTRQQQFIEEIKQLNYPKEMQTGKKKL
                     YYLATLGCQMNEHDSEKLAGMLSEMGYAESDNMNSSDLIIYNTCCVRENAEQKVYGHL
                     GPLKHLKRENPDLVIAICGCMMQQPEVVEHIKKTYRHVDLIFGTHNLYKFPELLFRAI
                     GSEKTVIDVWDSEGQIAEDIAIERKDGVKAWVTVMYGCNNFCSYCIVPYVRGRERSRN
                     LDNIVNEVMLLGRQGFKEITLLGQNVNSYGKDLGDDTDFAKLIREVDKVPGIERIRFM
                     TSHPKDLSDELIYAMRDCKKVCEHLHLPFQAGSSEILKKMNRKYSKEDYINLVEKVKE
                     SIPDITLSTDIIVGFPGETEEDFFDTLDIVERVKFDNAYTFLYSKRTGTPAARMENQV
                     PEDVKKERFQRLLELQNRISKEINDTFLGREVEVLVEGLSKNNDKVYTGRTRGNKIVN
                     FEGNKDMIGKLVNVRIDSVKTWSLEGTVI"
     gene            complement(4943..5809)
                     /locus_tag="PLA01_04120"
     CDS             complement(4943..5809)
                     /locus_tag="PLA01_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPD00519.1"
                     /translation="MDNNQKKLLKWLFETNAVRVCPQNKPFWYTSGTIGPYYINTHFL
                     YGSEDKANALLRLIDTEKVNELQCPEKVLAETLNNYESDDIYRGLIDELLAFIKNNID
                     IDEVDYVSGGERRDWFFSLIIAKFINKPHITIFKNLYSVITIDGKSEEITDLNGGKVL
                     HIADLITEASSYERAWIPAIKNNGGQIKWSVVVVDRKQGGKELLSSYGIESFAMVDID
                     IEFFKEVLDMGIINSAQFDMIEQYISDPKKSMADFLKKNPDFLEQALKADDKTKERAK
                     LCLEKDFYGLKK"
BASE COUNT         1672 a         1332 c         1060 g         1845 t
ORIGIN      
        1 attatctaaa aaagcatcct cccatattaa ctgaaatatt aactgaaaaa ttatctgaaa
       61 gagcatcgga tgaagaagat aaagacacgt tgcaggaaaa aagggactct aaaaaatgga
      121 tttttggaaa gcaacgggct tttgtattgg gagcatcggc aattgcatgc gtatatgccc
      181 tttgcatcac tattccatac atgggtttgg cggctatcgg ttcaaaccag ccttccatga
      241 aagccttcgc caacagagac gaagctgtaa acttcttttt acgagcaaag gattattctt
      301 catcttacag tagagaacta aataaatttt cacaatttct ttatgatttt aataaagtat
      361 ttattaacat tgcaataatt gcaaatccta acttttctct cgagctgccc agtatgggga
      421 tgaaagcaag ctctgatttt gcctcaggta tgggaggaca aaatacagca gggggaactg
      481 aagctgttta cagtgacgga atttataaag gcacctccaa aaccaatgtc caggtcgaga
      541 atgtggatga ggctgatatt gtaaaaaccg atggagagta tgtttattat atcaacggaa
      601 gcaatttatt catcatcagg tcaaatcctc ccgaacagat ggaaatagtg taccggcaca
      661 attttttgga aggcgaaccc agggaaatgt ttctatacaa cgattatata ggcgtaataa
      721 ttagtgaaaa acgcagaaca gtagtcaaaa catacaacat aaaggatcgc tccaatccgg
      781 tggctgaaag aactctggaa ttggattgcg gttatttgac ctcacgaatg ataaacaatc
      841 acctgtatct ggttacaaca tcatatgtaa gagacattga ttacccggaa tattccgata
      901 cttcagcaaa cgattccatt gttgaaatgg attataaaga cctgtttttg atgaacaacc
      961 ccagcaacga ttatgtcaaa atgaatgtag tcgctgcctt tcctatagat gatgccggaa
     1021 gtgaggctca ggtaaaggcg tatatcggcg gagacggtga gaatgtttat gtatctacta
     1081 attacattta tatcgctgat gaaatagccg atacaacagt tgtcaaccag cctataagag
     1141 actttttcga ctgtctttcg acggatgatt cgttatatta cgaataccaa tatgcaacca
     1201 atatatacag gatagaaata aatgacggaa ccatcggaaa atttgacagt gcttccgttc
     1261 gcggtgctgt acacaatcag tttgcaatgg atgagtacaa cggatatttc cggttaacca
     1321 ctcagaccgg cagatggagc agtgcctcga gcaatgtatt tgtcctggac gagaatatga
     1381 agctttgcgg ttctttaaag gggcttgctc cggaggagca tatttatgca tcaaggttca
     1441 tgggagacag actctatctg gttaccttta aacaggtaga tccccttttc gtaataagcc
     1501 ttgaagaccc gcgtgatccc agggttcttg gaaaactgaa aatacccggc tacagtgaat
     1561 acattcatcc cctcgatgaa aaccacatca taggtttcgg taaggatgct gtcgatagtg
     1621 taactggcaa ctttgcatgg tctcaagggg ttaaaattgc tatcttcgat gtcaccgatg
     1681 ttaacaatcc caaggagaag tttgttgaaa taataggaga caggggatcc gactcaccgc
     1741 ttcttcaaaa ccacaaggca ttgatgtaca tgaagaacct tgagcttatg gctttcccag
     1801 tatcggtagt agaaaactat cccgtgaatg attactacca atacaatgta tttcaaggtg
     1861 catatgtata taatgtaaac agtaccgaag gatttaaatt aaggggtagg attacgcatt
     1921 tgaaccagcc tttacaagat atttggcaaa gtgcccaaat tgacagaatt gtgtattccg
     1981 acggtgtatt atataccttg tcgggagaaa aggtaaaagc aacaaaatac acggatatga
     2041 aggatatgtc ggaggtgccg cttcgttaga ctgcggcggt tcggttaatt gaaaagatga
     2101 gcgtgcaata cctttgaaaa agggaaatat gtagtatccg tgaaaagggt gcatgtggta
     2161 tccgtgaaga aaagtattcc agaaacttaa gtttcgcatg tctagagcct gtaaatactc
     2221 ctcggggtcg agcaaaactc gcttcgcgta ggaggatgct ctccctttcc tcgtcgtatt
     2281 aacaggctct acaacttgct tcacaatgtt tcttccatat ttttcttcac ttaaaaacta
     2341 cgtgcccata gttaaaattc taatgctctt ccccatctat tgtaactgtc ctgctgcctc
     2401 cgtcccatcc aacattaaac cttagaccct ctgcaataaa ccgcacagga accagtgttc
     2461 ttccatttat aattgatgcg ggaacgtcaa gttccacctc ttgattatca ataaaagctg
     2521 ttttacttcc aatcttcagc acaactttcg aagaaccccg ggtgattgtg acggtatgtt
     2581 ccttctcatc ccatgatact ttagcaccca tttcctcaaa aatagctctt acaggtacaa
     2641 gggtccttcc gtcgactatc ctcgggtaca catcaaaatc cacttctttt ccgttaacct
     2701 tgacctttac agggtacaat accgtttcat cggcattgtt catcatatct tttatcatgg
     2761 caccggtacc ctgaataatt acgggattgc ttttggggtc aagccatttt aacaatttaa
     2821 gcatattatc ctcggatacg gcaatacaac cgctggtacc ggaatttgaa tccttccaga
     2881 tatgcaaaaa gattgcacta cctttgcccg caataaccgg attcatgttg tattctatta
     2941 ctattgaata cttgtagggc gttatattga gcctttcaaa agacttccac ctgtcattcg
     3001 ggtttcctct gtactccacc caggtattgt attctgcaga ggtaatgtca tcaatccaat
     3061 aatcgttttc tgtaaccttt ctgtagggaa gctttgtaat tgaggcaccg tacggtactc
     3121 cgaaggcatg tccaattctg aaaattccgg caggggcttt cccgtcgcct tcgatcttgt
     3181 ttgttgaaaa gccgtttttc cccacaactg ccttcattgt atcaaaggac ttcacccagc
     3241 cctcaggttt cctttcatag gcatcaacag cagccttata atcgccttca taatttgtag
     3301 aaaccactat tacctgcttt gacccgttta aatcaaacag tttctccaga aaggacgtta
     3361 tgctgtccgc ttccctaacc tcaacgaaac caaaaaatga gcccgctata catacaaata
     3421 aaaagcaaaa taaaagtctc ttcaatttaa acaattctcc ttttattttc tcttttttta
     3481 aatcgaatta tatgacggtg ccttccaaag accaggtctt tacactgtct attcttacat
     3541 tgacaagttt tccgatcatg tctttgtttc cttcgaaatt gacaatttta tttccccttg
     3601 tccttccggt ataaaccttg tcgttattct tgctcaatcc ctccacaagg acttcaactt
     3661 ctcttcctaa aaatgtatcg tttatctctt tgcttattct gttttgcagt tcaagaagcc
     3721 tttggaacct ctcttttttt acatcctccg gaacctggtt ttccatcctg gctgccggtg
     3781 tgcctgtcct tttggaataa aggaaggtgt aagcattatc aaactttacc ctctcaacaa
     3841 tatcgagggt gtcaaaaaag tcttcctccg tttcaccggg aaatccaact attatatctg
     3901 tggataacgt aatgtcggga atactctcct taaccttctc cacaagattt atataatcct
     3961 ctttggaata ttttctattc atctttttga gtatttcact gcttcctgcc tgaaagggca
     4021 ggtgcaaatg ctcgcacacc tttttacaat ccctcattgc ataaataagc tcatcggaca
     4081 aatccttcgg atgcgatgtc atgaaccgta tcctttcaat cccgggcacc ttgtccacct
     4141 cacgaatcag ctttgcaaag tcggtatcat ctcccaggtc ctttccgtag gagttcacat
     4201 tctgtcccag cagtgttatc tccttaaacc cctgacgtcc taacaacata acctcattca
     4261 caatgttgtc caggttccgg cttctttccc tgccgcggac ataaggtaca atacagtacg
     4321 aacaaaagtt gttgcagcca tacattaccg taacccaagc ttttactcca tcctttcttt
     4381 cgatggcaat gtcctcggct atctgcccct cgctgtccca cacatctatt acggttttct
     4441 ccgatccgat ggctctaaac agaagttccg ggaacttata caggttatgc gttccgaaaa
     4501 ttagatctac atgcctgtaa gttttcttta tgtgctccac aacttccggc tgctgcatca
     4561 tacagccgca tatcgctata acaaggtcgg gattctccct tttcaggtgc tttaaagggc
     4621 caagatgccc ataaaccttt tgctccgcat tttcccttac gcagcatgtg ttatatatta
     4681 tcaaatcact gctgttcata ttgtccgatt cggcataacc catttccgaa agcattcctg
     4741 caagcttttc ggaatcatgc tcgttcatct ggcaccccag ggtcgccaga taatacagct
     4801 tctttttacc ggtctgcatt tccttcggat agtttagctg ctttatctct tcaataaact
     4861 gctgctgtct ggtcatttcc tctgttgaaa catgtatgtt ttttctttcc ttagtgttgt
     4921 tatccaatta gctaacctcc aattatttct tcaatccgta aaaatccttt tccaagcaga
     4981 gctttgccct ctccttggtt ttgtcatcag cttttaaagc ttgctccaga aaatccggat
     5041 tcttcttcaa aaaatccgcc atggactttt tgggatcgga aatatactgc tcaatcatat
     5101 caaactgtgc actatttata atacccatat ccaaaacttc tttaaagaac tcaatatcta
     5161 tgtcaaccat ggcgaaggac tctattccat aagaagacaa cagttccttt cctccctgct
     5221 tcctgtctac aacaacaacg ctccacttaa tctgaccgcc gttattcttt atagcaggta
     5281 tccaggccct ctcgtaactt gatgcttcag tgataaggtc tgcaatatga agaacctttc
     5341 ccccgtttaa atcggttatt tcctcagatt tcccgtcaat tgttatcact gagtacaaat
     5401 ttttaaatat ggttatgtgc ggtttattaa tgaactttgc aataatcagc gagaaaaacc
     5461 agtctcttct ctctccaccc gaaacataat ccacctcgtc aatatcaata ttgtttttga
     5521 taaatgcgag caattcatct ataagtcccc tgtaaatatc gtcggattca taattgttca
     5581 atgtttctgc aagaactttt tccggacatt gcagttcatt tactttttcc gtgtcaatca
     5641 ggcgtaatag agcattggcc ttatcttcac tgccatatag aaaatgcgta tttatatagt
     5701 atgggcctat tgtacccgat gtataccaaa aaggcttgtt ctgcggacat actcttacag
     5761 catttgtttc aaaaagccac tttaataatt ttttttgatt attatccatt ttagtctcct
     5821 ccttctaatt ttttaaaata aatatttttt tttaaaatat taaacaattg ttttttttat
     5881 ggtataatat aaatgtaata atctataat
//
LOCUS       DAOOJC010000102         5896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_10986_length_5896_cov_4.212121, whole genome shotgun sequence.
ACCESSION   DAOOJC010000102 DAOOJC010000000
VERSION     DAOOJC010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10986_length_5896_cov_4.212121"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PLA01_04125"
     CDS             complement(<1..470)
                     /locus_tag="PLA01_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPD00520.1"
                     /translation="MKKVIFIIIVAVMLTFVLHSYATDESRLSYDSGNEIMLKNSRTI
                     IKQNLAVRKAFYQYNSSVQRTKGINTQWIDYETPFGKITIVYSPDIQVLFSKQTELLP
                     AQMKYYWNLTDSSRKMTEKALSLGFRDLYLGFMKADSDYQMSLKKLELQKRKYEA"
     gene            complement(600..1424)
                     /gene="proB"
                     /locus_tag="PLA01_04130"
     CDS             complement(600..1424)
                     /gene="proB"
                     /locus_tag="PLA01_04130"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPD00521.1"
                     /translation="MENSNLREDLKNSKRVVVKVGTSTLTYDNGEMNFARIERLVRAI
                     SDLKNQGRDMILVTSGAIGVGVGKLKLGSKPSTIREKQAVAAVGQCELMHIYGKFFQE
                     YGHTVGQILLTRDVVENETGKVNVVNTFEALLEMGIVPVVNENDSVAVDEIESGIHRV
                     FGDNDSLSAIVAKLVKADLLIILSDIDGFYDCDPRTNKDSKLISVIKEITPDVEKCAG
                     GVGTSRGTGGMITKLTAAKIATAAGVNMVIANGDNPKVIYDIIGGKTIGSLFAGRK"
     gene            complement(1732..2454)
                     /locus_tag="PLA01_04135"
     CDS             complement(1732..2454)
                     /locus_tag="PLA01_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HPD00522.1"
                     /translation="MYEERRYRNMFKGINLKFFDVCIKETDIRIGADRELRKEAYELA
                     LNCRRDIEEYIADHQRFLESLEPIDCSNDAPDIIKRMCEAAKKAGVGPMAAVAGAVSE
                     FVGMGLLKMCNEVIVENGGDIFMKSDVPRKVGVYAGKSRLSEKIALEIMPDKTPLGIC
                     TSSGTVGHSLSFGRADAVVIVSKDTFLSDAVATATGNVVKDEGYIEKGIEFASSIDGV
                     DGVLIIVGDSLGAWGDIKLVKL"
     gene            complement(2818..3225)
                     /locus_tag="PLA01_04140"
     CDS             complement(2818..3225)
                     /locus_tag="PLA01_04140"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HPD00523.1"
                     /translation="MKKIKVSLYFPAAEVTKPITYHLIKDYDLQINILNADISLNRQG
                     KLVMDMIGSEWNIESGLKYIEDQGIKYKLFTKTIIWQEEGCVHCGACTAVCPSGALQM
                     DKESWSLTFEKEKCMICELCVKACPLKVMCVSI"
     gene            complement(3212..4387)
                     /locus_tag="PLA01_04145"
     CDS             complement(3212..4387)
                     /locus_tag="PLA01_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HPD00524.1"
                     /translation="MGYKSFDEINEKIKCGKAVVATAEEIIDIVTEKGVKQAAKEVDV
                     VTTGTFGPMCSSGMFINFGHSEPPIRMTKVWLNDVSAYAGIAAVDAYIGATELSETRG
                     MEYGGAHVIEDLIAGKDIKLYAESYGTDCYPRKEITTYINKNNINQAYMFNPRNVYQN
                     YAVAANTTNRIIHTYMGTLLPNLGNVTYTTSGELSPLLNDPYYRTIGIGTRVFIGGAQ
                     GYVAWEGTQHNPGQKRGENGVPLSSAGTLALIGDIKQMDTRFIRAAVFEKYGVSMFVG
                     VGIPIPILDEDMLIMTCIKNSEILANIYDYGVQSRNKPVLGSVSYEELRSGSIEISGK
                     KVPTAPLSSLKKAREIAELLKQQITKGEFFITQPVASLPLENKANTLDIRGLENEKD"
     gene            4604..4990
                     /locus_tag="PLA01_04150"
     CDS             4604..4990
                     /locus_tag="PLA01_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00525.1"
                     /translation="MDKYKKLETITNGLNAANKIMTLQTSAINQRSRDSAYINPALLS
                     QILHVIAQYSPDKNKMLLTRSLEKTNRYSKAIRELKEEVLNIREKNRIYRDDVIKTLH
                     IIKPIVDPNRQTIIDKILKIQEILDS"
     gene            complement(4979..5329)
                     /locus_tag="PLA01_04155"
     CDS             complement(4979..5329)
                     /locus_tag="PLA01_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00526.1"
                     /translation="MGDTFLFNFFKTLILTGVVAIVFGFIWLVALYMLADKEVMQNRA
                     EHTTALGNALLVMHSIFEPGKRPQTEQVIWVKRRRTPVEKGVLGLTELNYDKVFIKGY
                     MPLKNKFFRKKSGV"
     gene            complement(5532..5607)
                     /locus_tag="PLA01_04160"
     tRNA            complement(5532..5607)
                     /locus_tag="PLA01_04160"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5572..5574),aa:Pro,seq:cgg)
     gene            complement(5701..>5896)
                     /locus_tag="PLA01_04165"
     CDS             complement(5701..>5896)
                     /locus_tag="PLA01_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HPD00527.1"
                     /translation="GRRDYRIMIVTSEFHLFRSKILARHNGFTAYGIPAPTNPYELPN
                     SFIREYFAVVKSMVFDIMLD"
BASE COUNT         1780 a         1396 c          898 g         1822 t
ORIGIN      
        1 gcttcatatt ttctcttctg aagttcaagc tttttaagac tcatttgata atcactgtca
       61 gccttcataa agcccagata caagtcccta aatcccagtg aaagtgcctt ctcggtcatt
      121 tttctgctgc tatcggtcaa attccagtaa tacttcattt gagccggcaa cagctccgtc
      181 tgttttgaaa aaagcacctg tatatccgga ctgtaaacaa tggtaatctt tccaaaagga
      241 gtttcataat caatccactg ggtatttatg cctttagtcc tctgaacaga actgttgtat
      301 tgataaaaag cctttctcac tgcaaggttt tgttttatta tggttctgct gtttttcaac
      361 ataatctcgt taccgctgtc ataagacaaa cggctttcat ccgttgcata cgagtgcaac
      421 acaaaggtta gcattacagc tacaattata ataaatatta cttttttcaa ggaataccct
      481 ccttccgttt cgaaaatggc atgctttgaa gttttattaa tatgtcactt ttttatatgt
      541 aattatttta tggaagaaca aactatccga caagggtatt gcaataacct gcaaccgatt
      601 tattttcttc ctgcgaacag cgaacctata gtcttacctc ctataatgtc ataaataacc
      661 tttggattat ctccatttgc aataaccata ttgactcctg ccgcagtcgc aatcttagca
      721 gctgtcagct tggtaatcat tccacctgta cctctcgaag ttcctacgcc tccggcacac
      781 ttttccacgt ccggcgttat ctcctttata acagaaataa gcttcgagtc tttatttgtt
      841 cttggatcac aatcgtaaaa gccgtcaata tccgagagaa ttataagcaa atcggctttt
      901 acaagctttg caacaatcgc agacaatgaa tcattgtccc caaacacgcg gtggattccc
      961 gattctattt catccactgc aacagaatcg ttttcattaa ccaccggtac aattcccatt
     1021 tccagcaaag cctcaaaagt attaacaaca ttgactttac cggtctcatt ttctaccaca
     1081 tcccgggtta ataaaatctg ccccaccgta tgaccatact cttgaaaaaa cttgccatat
     1141 atatgcataa gctcgcactg cccaacagca gctacagcct gtttttccct tattgtactg
     1201 ggcttggagc ccagctttag tttcccgacg ccaacaccta ttgcacccga tgtaaccaat
     1261 atcatatctc tgccctgatt tttaagatcc gaaattgctc tgacgagtct ttcaatcctg
     1321 gcaaagttca tctcaccatt gtcatatgta agagttgaag ttcctacctt caccaccact
     1381 ctttttgagt ttttcagatc ctcacgcaaa ttactgtttt ccattttctt tcccctaccc
     1441 attatttata aaaattttaa ttaatcactg tcctgttatc gaacgcagaa agtcaattat
     1501 attcccaacc atacattcaa taacaataga acaaacgatt ttatataatc cgaataaaat
     1561 atcggagaat atttctgcaa aacaaacata aaaaacatca ataagaatat tatacaacta
     1621 ataatatcaa atttcaaaat aaaaaccgga aaatcaaaga taattttaat ccttttaata
     1681 aatacaggtg tttgctttaa aaatgttaaa gtcaatgtaa aaaaaatagt cttaaagttt
     1741 aaccagtttt atatctcccc atgctcccaa actgtcccct actataatga gaacaccatc
     1801 aacaccgtca atggaagaag caaattctat ccccttttca atgtaaccct catccttgac
     1861 tacatttccg gtagcagttg caacagcatc agatagaaat gtatctttgg acacaattac
     1921 taccgcatca gccctgccaa aacttaaaga atgtcctact gttcccgaag atgtacatat
     1981 tccaaggggt gtcttgtcag gcataatttc cagtgctatc ttctcactca gccttgactt
     2041 tcctgcatat acgccgacct ttctcggaac gtcggacttc ataaaaatgt caccgccgtt
     2101 ttcgacaatt acctcattgc acatctttaa aagccccata cccacaaact cgcttaccgc
     2161 tccggcaact gctgccattg gccccactcc tgcctttttc gctgcctcac acattctctt
     2221 tataatatcc ggggcatcat tgctgcaatc aataggctca aggctttcaa gaaatctttg
     2281 gtgatctgct atatactctt caatatccct tctgcaattt aaagccagct catacgcttc
     2341 tttacgaagc tccctgtccg ctccgattcg tatatccgtc tccttaatgc acacatcaaa
     2401 gaatttcagg tttataccct taaacatgtt tctgtatcgt ctttcttcgt acatatttat
     2461 tatctcccat atatatccag catattcata aatgcaaatt atattttata gtctgatatc
     2521 ggcagccaac aacaatctga aaagccacct ccttcaaaat aaaaatcctt cacgattata
     2581 tccttcacaa cctttattat ttaagaggca tcgacaaact caagaaagac atacttgagc
     2641 ttaaaaactt cacaaccatt ataatttaag gggtatcctg cccgatatct caaggcctga
     2701 ttcccccaat aaacgtagct gacacaaatt ataacgtaaa gcttctttta ctcacttttt
     2761 gcaacactta agttaattcc cgccacttat aagaagtatg ggctgtcttc tgcctcatta
     2821 tatcgacacg cacatgacct taaggggaca tgccttcaca cacagctcgc aaatcatgca
     2881 cttttccttc tcaaaggtaa gactccagct ttctttatcc atttgaagtg ctcccgaagg
     2941 acatacagcc gtacatgcac cgcaatgaac acatccctct tcctgccaaa ttatggtctt
     3001 tgtaaagagc ttatacttta taccctggtc ctcaatatac ttaagtcccg attcaatatt
     3061 ccattcgctt cctatcatgt ccatcacaag cttcccctgc ctgttcaggc ttatatccgc
     3121 attcaaaata tttatctgaa gatcatagtc ctttatcaga tgatatgtga tgggtttggt
     3181 tacttcagct gcaggaaaat ataaagaaac cttaatcttt ttcattttct aaccccctta
     3241 tatcaagggt attagcctta ttctccaaag gcaatgatgc caccggttgt gttatgaaaa
     3301 actcaccctt cgtaatctgc tgtttcaaaa gctctgcaat ctctcttgcc tttttaaggc
     3361 ttgaaagcgg agctgtcggc acttttttcc cactgatttc aattgagccg ctgcgaagct
     3421 cctcgtaaga aaccgatccc aaaacaggct tgtttctcga ctgaactccg taatcatata
     3481 tattggcaag tatctcactg tttttaatac aggtcataat gagcatgtcc tcatcgagta
     3541 tcggtatcgg aatccccaca cccacaaaca tggacacccc gtatttttca aataccgctg
     3601 cccgtatgaa gcgggtatcc atttgcttaa tatctccaat cagtgccaat gtacccgctg
     3661 aagacagcgg aactccgttc tctcctcttt tctgtcctgg attgtgctgt gttccctccc
     3721 atgcaacata accctgggca ccgccaataa atactcttgt gccaatgcct attgttctgt
     3781 agtaagggtc atttaaaaga ggacttaatt cccccgaagt ggtatacgta acatttccga
     3841 gattcggaag caatgttccc atataagtat gaattatcct gtttgtggta ttggcggcaa
     3901 cagcatagtt ctgatataca tttcggggat taaacatata ggcttggtta atattgttct
     3961 tattaatata agtagttatc tcttttctag gatagcaatc ggtgccgtag ctctcggcat
     4021 aaagctttat atcctttccg gcaatcaggt cttcaattac gtgggcaccg ccgtattcca
     4081 ttccccttgt ctccgaaagc tccgttgctc caatatacgc atccacggca gcaatacctg
     4141 cataggcaga aacatcattg agccacacct ttgtcatcct tatcggcggc tccgaatgcc
     4201 cgaagtttat gaacatccct gaagaacaca taggaccgaa agtacctgta gtcacaacgt
     4261 caacctcttt tgctgcctgc tttacaccct tctcggtcac tatatcaatt atctcctcag
     4321 cagttgcaac aaccgctttt ccgcatttta tcttttcgtt aatctcatca aaacttttgt
     4381 accccatttt atcgcacctt cttacaatag tataattact attataggtg tttatcgaac
     4441 atatttcaat tgtctatttt aaatgggtac atcaaataaa tgtaaagaaa aatattccaa
     4501 aaactattat atattataat tgggacattt acagtattag taacgatatc gttatattta
     4561 acataatata ataccaggaa ttttttaaga tcaaggagta tgtatggata aatacaaaaa
     4621 gcttgaaacc attacaaacg gactaaatgc cgcaaacaaa atcatgacgt tgcaaacctc
     4681 tgcaattaat cagaggtcga gggattcagc atatattaat ccggcattac tcagccagat
     4741 tctgcatgta attgcacagt attcaccaga taaaaataaa atgctcttaa caaggagttt
     4801 agagaagacg aatcggtata gtaaggctat cagagaactt aaggaggagg ttctgaatat
     4861 cagggaaaaa aacaggattt atagagatga tgttattaaa accctacata taataaagcc
     4921 gattgtggat cccaaccggc agacaataat agataaaata ctgaaaatcc aagagatact
     4981 agactcctga tttctttctg aaaaacttat tttttaaagg catataccct tttataaaaa
     5041 ccttatcata gtttaattca gttaatccca gaactccttt ttccacagga gttcgtcttc
     5101 tttttaccca gattacctgt tcagtctgtg gccttttgcc cggttcaaat atggagtgca
     5161 taacaagaag ggcattgccc aacgcagttg tgtgctcagc ccggttttgc ataacttctt
     5221 tatccgctaa catatataat gctacaagcc atataaaacc aaatacaatc gccacaactc
     5281 cggttagtat caatgtttta aaaaaattaa ataaaaacgt atctcccatg ttatcccacc
     5341 taatcagata taatatgttt ttactcttag acttatttta acacaaatac tgtcaattct
     5401 gcaaggcaac tgaacaaagt gcttttaagt aataactcac ttaaagaaga gttaagaaaa
     5461 gtatattggc atcagtcaac tatgagtata taaatcaaaa actcccaaag gcacaaatcc
     5521 tttgagagtc atggtcggag tggcgggact cgaacccacg gcctcttggt cccgaaccaa
     5581 gcactctacc acctgagcca caccccgtta accacacaat atattttaac agcattattc
     5641 gatattagca agagtatttt tattcaaatt tgttatgttc aaaaaatatc ttctatattc
     5701 ctaatccagc attatatcga acaccatgga ttttacaacg gcaaagtact cccggataaa
     5761 tgagttgggc agttcatagg gatttgttgg agccggtatt ccataagctg tgaaaccgtt
     5821 atgccttgca agaattttcg accggaacaa atggaactca ctggttacga tcattatccg
     5881 atagtcccgt cttcct
//
LOCUS       DAOOJC010000103         5843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_11112_length_5843_cov_3.129233, whole genome shotgun sequence.
ACCESSION   DAOOJC010000103 DAOOJC010000000
VERSION     DAOOJC010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11112_length_5843_cov_3.129233"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLA01_04170"
     CDS             complement(<1..289)
                     /locus_tag="PLA01_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00528.1"
                     /translation="MKNRRKRTCGYAANAAAARALMSSEVNSKRYREYVEFIFNTLKI
                     SGPDSYEKLLDLMHKNEVYVEYLAEKIADREDSEELFRFVNAFINTKTARKS"
     gene            complement(397..810)
                     /locus_tag="PLA01_04175"
     CDS             complement(397..810)
                     /locus_tag="PLA01_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPD00529.1"
                     /translation="MTGYHIPTKEVRTYRLDKITQLRSNLEFDYYMEKGFDIKKHLEN
                     AVFMHADSHKIHVRLRCEMRILDDVIERFDNCRLDKDLTSENHFFATIPDTTMLGMKY
                     WALEFSSACEVLEPEELRLEVRNTLARALAWYDEK"
     gene            complement(849..1430)
                     /locus_tag="PLA01_04180"
     CDS             complement(849..1430)
                     /locus_tag="PLA01_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00530.1"
                     /translation="MGPNLMLPLYILEFMTEHTDETNALLPTEIVKGMKKRYGNEIVS
                     KTETVVENIEKLNLFFKSRYDGVKIIEAVNGIGNGRYKRNLRYFLSARKFDFSEILFL
                     HKLLLNSNAISQDAIQDMCSKLSDFLSYEQRKLLKQNIVTDGSMKTRNKAVFINLENI
                     LKSIELKENIEFTYNEYNLEKKLVPRVESILIS"
     gene            complement(1524..2423)
                     /locus_tag="PLA01_04185"
     CDS             complement(1524..2423)
                     /locus_tag="PLA01_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00531.1"
                     /translation="MITMNHIKTQCTAKGCKTCSSNLYLLAQHIYDSHMGDIIDILNS
                     FSYPERSQLLMYITGMMKRSIGEESDEVFLTDNSMSGDSSVFLLNDYINPYYPISREK
                     RQYSLYLHNKIKEMDKDVLKAIGLTDCSIEGVFLEAAFMRDYWYTDKEEFNRTLKEYI
                     EKKFDACIDGGSIGSAHPKNWQVDVPYPRWMMNAKPDIAVVVKKDNEYRLHFIECKYF
                     HGEGKYSGMEADSNEIAGYKTENQENIIEFLCDHLCLKYRDSSGMENPIQRGKVIKVQ
                     FVSEQYNKKYENYAVVEIKFLLK"
     gene            complement(3027..3632)
                     /locus_tag="PLA01_04190"
     CDS             complement(3027..3632)
                     /locus_tag="PLA01_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00532.1"
                     /translation="MKTKESGTYESKDGVQFITKDFIIEHKMSQYIKFEGLLKKIGED
                     KEFFDGKAKENKDKIFEWNPEYIEVYENTVYTIIRESTLESRFPYLYVAKLDLEKLYN
                     GNSPTFEINGKTYYGELVKRISTKEESVVMWHGQKLRPDIGSYKRDGVIQFTVPIKLT
                     DKTITRYVMVNPENDEVKIYDSLRSGAEVIYENGAFIKGLL"
     gene            complement(3629..4336)
                     /locus_tag="PLA01_04195"
     CDS             complement(3629..4336)
                     /locus_tag="PLA01_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00533.1"
                     /translation="MMKNVKILAIILAAVMLFTITGCSKDVNNSDEEEAAEAAVIDED
                     IDIPKDPDHAPVSRLEEEGGSIGLTATNYNITNWVKDDKKVKMLKKLNKQKKYVTSEH
                     NFIYKNYLYDMYDFVAAVPEVIKRINLDNPQEAETINITGLMQADGRHGSVYDWIFHD
                     GYVYLTNLGYEKPEDQHEDKYCVFVHRLNPENGKVDMDYYKVEVRYVVKNYIPDIIND
                     KLNITGLCQDIRDGLLT"
     gene            complement(4559..5650)
                     /locus_tag="PLA01_04200"
     CDS             complement(4559..5650)
                     /locus_tag="PLA01_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00534.1"
                     /translation="MAQKGRVKKVFPGGNTSRGFYSFYDYIIPEDAERIFVIKGGPGT
                     GKSSFMRTIGEDFLKLGYDVELHYCSSDNKSLDGVVIKKANVALLDGTAPHVVDPKHP
                     GAVGEILNLGEYWDESRLVKDKYYIINCNKQIKMRFESAYRYLGAAKEMQDDMEEIIG
                     EGVDRGKLNKLKTELRYELVDGVSAINTNAAVRHLFDSSITPEGLVDYVESIIQSNYI
                     CYYLKGGVGTVSTEILSFLADEYTMKGYGVELYHQPLNPDRLQTLVVEGLKVGITINP
                     KTEMKAYKIIDLDSIIDEDKFKDKAELLQKDAETYLRMLEEGVRRIKMAKLIHDDLEK
                     KYVTCMNFEAVTELRNRTVERIKGILRNK"
BASE COUNT         1623 a         1309 c          866 g         2045 t
ORIGIN      
        1 cactctttct cgcggttttg gtatttatga atgcatttac aaaacggaac agctcctcac
       61 tatcctccct gtcagcaatt ttctcagcta aatattctac gtacacttca ttcttatgca
      121 tcaaatccaa cagtttttcg tagctgtccg gaccgctgat ttttaaggtg ttgaaaatga
      181 actcgacata ttctctatat cttttgctgt ttacttccga agacatcagc gcccttgcag
      241 cagctgcatt tgctgcataa ccgcaggttc tttttcttct gttcttcatc atatttcacc
      301 cctttatctt gttaaggaat ttaatctgtg ctgtaataat aacaaaacaa gctcccgctt
      361 tttcgggagc ttgaattcat cattatatat tatattttac ttctcatcat accaagctaa
      421 ggctcttgcc agcgtattcc tgacctccaa acgaagttct tccggctcca gaacctcaca
      481 ggccgagctg aactcaaggg cccagtattt catacccagc attgttgtgt ccggtatggt
      541 tgcgaaaaag tggttttcac tggtaagatc cttatcgagc cggcagttat caaaacgttc
      601 aatcacatca tctaaaattc tcatctcgca tcgaagcctg acatgtatct tatggctgtc
      661 agcatgcata aatacagcgt tttcgaggtg ctttttaata tcaaaacctt tttccatgta
      721 ataatcaaat tcaagattgc ttctcaactg tgttatctta tccagcctat aggtacgtac
      781 ttctttagtt ggtatatgat atcccgtcaa aaagtagctt ccgtatgagc atgtgatatc
      841 atatgggctt acgatataag gatactttct acccttggga caagcttttt ctccagatta
      901 tattcgttat atgtaaattc aatgttttct tttaactcaa tggatttcag gatattctca
      961 aggttaataa atacagcttt attacgtgtt ttcatactgc catcagtaac tatattctgc
     1021 ttcagcagtt ttctctgctc ataacttaga aaatcagata attttgaaca catgtcttgt
     1081 attgcatcct gactgattgc gttagaattt aataatagtt tatgcaaaaa caatatttca
     1141 ctaaaatcaa actttcttgc ggaaaggaaa taccgtaaat tacgtttgta tcgtccattc
     1201 cctataccgt ttacagcttc aataatcttg acgccgtcat aacggctttt gaaaaaaaga
     1261 ttcaatttct caatgttttc aaccactgtt tcagttttgc tgactatctc atttccgtac
     1321 cttttcttca ttcctttcac tatttcagtg ggtaataatg cgtttgtttc gtctgtatgc
     1381 tctgtcataa actcaagaat atacaaaggt aacattagat ttggtcccat acaatacccc
     1441 ccaatgatta acttccgcca agaattacat atattatata tggtattaat acaagctaac
     1501 aagctgacag gccgatacct gatttactta agcagaaatt tgatctcaac aactgcatag
     1561 ttttcatatt tcttattata ttgctcggaa acaaactgaa cctttataac ctttcctctt
     1621 tgaatgggat tctccatccc cgaactgtcc ctatacttta ggcataaatg gtcacaaaga
     1681 aattctatga tattctcctg attttcagtc ttatatcccg caatttcatt actgtcagct
     1741 tccataccgc tgtacttccc ttccccgtga aagtacttgc attcaataaa atgcagccta
     1801 tactcattat ccttcttcac aaccacggca atatccggtt tggcattcat catccaccgc
     1861 ggatagggta catcaacctg ccaatttttc gggtgggctg aacctatact tcctccgtca
     1921 atacaggcat caaacttttt ctcaatatac tctttcaggg ttctgttaaa ttcctcctta
     1981 tcagtgtacc aatagtctct cataaaggcg gcttcaagga atactccctc aatggagcaa
     2041 tcggtgaggc ctatagcctt aagtacatcc ttgtccattt cctttatctt attgtgcaga
     2101 tatagcgaat actgcctttt ctccctggag atagggtaat aaggatttat gtaatcattt
     2161 agcagaaaaa ctgaactatc gccggacatg ctattatccg tcaggaatac ttcatcagat
     2221 tcttcaccaa tactcctctt catcattccg gtaatataca tgagaagctg tgatctttca
     2281 ggatagctga agctgtttaa aatatcaatg atatctccca tatgtgaatc atagatgtgt
     2341 tgtgcaagca gataaagatt cgatgaacag gttttgcagc ccttggctgt gcattgagtt
     2401 ttgatgtggt tcatagtaat catgctccta aaatcatact ttttgtcagt tactgttgca
     2461 gaatataatt ttccaagtaa agcaccaaat aagaaatcca ataaaatcct accaaatttt
     2521 ttgcttcatg tccaatactt acgggggtga aggtatttta cagaactgag gatgctttat
     2581 tcccataaat attggatttt ttgtataaca acaccaaaag tcattttcga agaattttgt
     2641 cgaataaaaa tgagaatttt taaaaggaat gtttttattg atatcgaatt caaaatcaac
     2701 aaagccagga ggtgcttgca tgaaattaat taacagtcgg ttacacaaat atataccata
     2761 tcgagaatgc agaacagatt aagaatttta tagaaagtaa gattcaaaag ccataaagta
     2821 tctatgacat cgaatgtact ctgtaactaa acaaaaggta atgtaaataa ttctgtgggt
     2881 gaatgaaaaa gttaactcct ttccggttta aatttgatta ttgcagaaag gagtttactt
     2941 tccattcacc cacagacttt cattaggtca ttgaatccac aatcttaatt cttgcttctc
     3001 taaatcaatg aagtgctctc actttatcat aataagccct ttataaaagc tccgttctca
     3061 tatatcactt ctgcaccaga ccgcagggag tcatatattt tcacttcatc attttctgga
     3121 ttaaccatta cgtatcttgt aattgttttg tccgtcaatt ttattggtac ggtgaattgt
     3181 ataactccgt ctcttttgta agaacctatg tctggtctta gtttttgacc atgccacata
     3241 accacactct cttcttttgt gcttattctc tttacaagtt ctccgtaata tgtttttccg
     3301 tttatttcaa atgtcgggga attaccgtta taaagtttct ctaaatccaa ttttgcaaca
     3361 taaagatatg gaaaacgaga ttctaatgtc gactctctta ttattgtata aacagtattt
     3421 tcgtatactt ctatatattc tggattccac tcgaatattt tatctttatt ttctttcgct
     3481 tttccatcaa aaaattcttt gtcttctcct attttcttca gtaatccttc aaatttaatg
     3541 tactgtgaca tcttatgttc tattataaaa tcctttgtaa tgaattgaac tccatctttt
     3601 gattcatatg ttcctgattc ctttgtcttc atgtcaataa accatcccgg atatcctggc
     3661 agagacctgt tatatttaat ttgtcattaa taatatctgg aatataattc tttaccacat
     3721 acctcacttc aaccttataa taatccatat ctacttttcc attttccgga tttaacctgt
     3781 gaacaaatac acaatactta tcttcatgct gatcttccgg cttttcatat cccagatttg
     3841 tgagatagac atatccatca tgaaatatcc agtcatatac actgccatgc ctcccatcgg
     3901 cttgcattaa ccctgtaata tttattgttt ctgcctcttg tggattatcc aaatttattc
     3961 tttttattac ttcgggaact gccgctacaa aatcatacat atcatataaa tagtttttat
     4021 aaatgaaatt atgctctgaa gtgacatatt tcttttgttt attgagtttt ttcagcattt
     4081 taactttctt atcatctttt acccaattcg tgatattgta atttgtagct gtaagaccga
     4141 tacttcctcc ctcttcttca agccttgaga caggagcatg atcagggtct tttggaatat
     4201 cgatatcttc atctattaca gcagcctcag ctgcttcctc ctcatcagaa ttattcacat
     4261 ccttagagca tccggtaatt gtaaacagca ttacagcagc taatattatt gccaagattt
     4321 ttacattttt catcatgtgt tttcctcctt gatatcattc taaacaatag tttacataag
     4381 tgttatactg ttccagacat ttatcttaat aatacaaaac ttgacatact ttagcaacta
     4441 tttacatcca tattatttat agtaccaatg tatttccaat aactactttg ccatcctcct
     4501 cttggagcaa accagaacct ccgggtatat caactccgga ggttctgatt tgtttaccct
     4561 atttatttct caatatcccc tttatcctct caaccgttct attcctcaat tccgtaactg
     4621 cctcaaaatt catacatgtc acatattttt tctccaggtc gtcatggata agctttgcca
     4681 tttttatcct cctgacgcct tcctccagca tccggaggta tgtttccgca tccttttgga
     4741 gaagctctgc cttatccttg aatttgtctt catcaataat agaatccaga tctattattt
     4801 tatacgcctt catctctgtt ttgggattta tggttattcc cactttcaga ccctcaacaa
     4861 ccaaggtctg cagtctgtca gggtttaaag gctgatggta taattcaacc ccatagccct
     4921 tcatggtata ttcatcagca agaaaagaga gtatctcagt agagactgtg cctactcccc
     4981 cttttaggta atagcagata tagttgctct gtattatgct ttcaacataa tccaccaaac
     5041 cttccggagt tattgaactg tcaaaaagat gcctgacagc tgcatttgta ttaatggcac
     5101 tgaccccatc caccaattcg tacctcagtt ccgttttaag cttattcagc ttccccctgt
     5161 ctactccctc tccgataatc tcctccatat cgtcctgcat ttcctttgct gcccccaaat
     5221 acctgtatgc actctcaaac cgcattttta tctgcttatt gcagttaata atgtagtatt
     5281 tatccttcac aagccgcgac tcgtcccaat actccccaag attcagtatt tcaccaaccg
     5341 cacccgggtg tttgggatct actacatgag gagcggttcc atccagcagt gcgacatttg
     5401 ccttttttat caccactccg tccagggatt tgttatctga ggagcaataa tgaagctcca
     5461 catcatatcc cagtttcaga aaatcctctc ctatagtcct catgaagctt gactttcctg
     5521 tcccggggcc tcctttgatt acaaatatcc tttctgcatc ctcgggtatg atatagtcat
     5581 agaatgagta gaatcccctg cttgtgttgc ctcccggaaa aaccttcttt accctgccct
     5641 tttgtgccat gcttatacct ccaaaagaaa aatcaaatta ctatatcata tgcaattcca
     5701 cagatttatg ccataaagtt tgtatatcat gggctaaggg gcaggctcct catcccaggc
     5761 acttatcgag ggtcgtttta tcagcagctc ccagcaggta tccgtttgct ccgatttcaa
     5821 atgcatcaag agcataatcc cgg
//
LOCUS       DAOOJC010000104         5838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_11123_length_5838_cov_4.136434, whole genome shotgun sequence.
ACCESSION   DAOOJC010000104 DAOOJC010000000
VERSION     DAOOJC010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11123_length_5838_cov_4.136434"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLA01_04205"
     CDS             <1..216
                     /locus_tag="PLA01_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00535.1"
                     /translation="WLVFISSNGSAIRKVSEISNIYKEFSPTRSKHFIRIDFTDGSSF
                     ANMCDSACDEIIELIKKEFPSITSRTK"
     gene            302..640
                     /locus_tag="PLA01_04210"
     CDS             302..640
                     /locus_tag="PLA01_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00536.1"
                     /translation="MLKSKMTKGISLCLAVMMLLLGVPLNGFAAVTDEISGQQEGSIP
                     VEVFTQSNDMEPEKKSEGLDDSSAYSSDSAPSGYVGDNTFLDVPQRRMMGMAKANAID
                     TQKKSSSHRR"
     gene            981..2780
                     /locus_tag="PLA01_04215"
     CDS             981..2780
                     /locus_tag="PLA01_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00537.1"
                     /translation="MTNNDSLTDSDLASYYCSHISFNEESLPPGTTVILRETRTNKPT
                     LIEYPLGKGRVIASGLTWEHNYNHWGRTINGVKVGNFAKAMDDMFSYAIRVSSIDVKE
                     LHLLKEWRLKKIAHSITVADGSSQSGEFLPIAGATVTIDGKKYVTDENGQVFYNENYG
                     LHTAVVSASGYYERKLMYNMQERTSRIFFLEKKVNDGLPYIIQTNGVRNYSGIYLDLR
                     DQALHYTENRTDVAALVIDGNWNGHGAGRYVIYQDPVVGEPGKYISSTTGVFTFSPGM
                     VFKPNRQVKLKMIAGDGKESRPINLKLVIDRAPSQYGGGNNGGGNGGGNNGGNNNAGL
                     KTGVDKFDWIGNHPVQSDNDIFTKILTTDMSISSELIPIEISSERDDDGTTIYKGVIG
                     FISGSATKDLLTGKGEGFAIESPWDEPKKQIKGYKTAGNPKAYFNNLKRKYGKDWKPS
                     KLSATFDVEVNACGYLELKVGQNGNIISSDGGIVIDASGDFTIGRTFMAGPVPVYFEI
                     KPGIGLEVNAGIEFYNADAGGLGFRPHFGSLTLEVPHITLEGGVGVRGVATVGLQGSG
                     SLNIEFEPGNKTNGSLEFGGAIRVKVIFVVDYK"
     gene            2826..>5838
                     /locus_tag="PLA01_04220"
     CDS             2826..>5838
                     /locus_tag="PLA01_04220"
                     /inference="COORDINATES: protein motif:HMM:NF015407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="X2-like carbohydrate binding domain-containing
                     protein"
                     /protein_id="HPD00538.1"
                     /translation="MYRLFAARSLSFEEAELSLASRDYLDDCTAWNGGENKFLKLSAN
                     SADTTIKTLQEGVMPDALPQLQKVGDKLVMLFLRDVPERMTGNHTQLVYSVFDGGVWS
                     EPKPVWDSETADFFFESVVDDGELYVVWQKLKSVPTKETPEDLLAEVAQNSEICFAKW
                     NDETNEFTSQSYLTDDDELDMYPNVAVRDDLVSVVWVTNDENDPTGESGNYIIKRKDI
                     INDAMGDVSELYSTTEFVTEMAAGYSDEELHILYAVLEEDAEKSNIYLLKGGASESLT
                     SGNESASLRFVNNVFLWQSEGAIYRYDPTTQVLAQIITGENGSASSSYKYASNGTKSA
                     IVWVDSEKNADTGKDSSVLKASIFADGEWSSPVVLLNNLDTNISYMDIELLENGEYAA
                     ILNTASFSEDQEVKTSLKFAAIYPHNDVELLYAAVDKANAQSNKQSLTIYLANNGDSV
                     VPSIELSVDANGNNYLYKTLDVSLSPGEQIVLTEDIDISSINSVTAADVTVNIDEDSE
                     PDNNRAQITLGHVDVSLSLDTYDLGDSVVFALTARNEANTVAHSAISIIEDSPEGIVL
                     DVKNIGEVGCDDSVQYLYQIDKTQVQFDEFNSKTYFFNLESLEEDWNTEDNQLFYVVS
                     QNPDETDVDPEGIMEEVKIVQPTSVDISVNKLHFDSVDSDSVQLSADIYPANSSINNI
                     LWSVEDPDIVYVDSEGLVTPLRPGTTTITSKVSDEVYDTITVTVADPSTLYKISAKAG
                     TGGTVSGAGTFASGTEVKLTAKPNTNYSFEGWYEDGKKIDGTGAAYTFKVSKDRILEA
                     RFIYDVSGGGGYTGGGDKSISAALDVSEFKFDKSSPTDITVKLSSGSYTFREIKNGSY
                     TLIPHQDYIVNGNMFTIKAAYLETLEDGQATLTFTMSGGNNPVLTVTVESGVSPVTEG
                     DGVLLNGKETNLKFNPDGSVTINEEDISELSKPVILTLPYKDGGNSHAGVLKKDGQDV
                     VMPFSVYKDNTMIILISEPGTYGVVNNKKNFN"
BASE COUNT         1839 a          989 c         1325 g         1685 t
ORIGIN      
        1 tggttggttt ttatctcttc taacggttct gcaatccgta aagtatccga gatatccaat
       61 atatataaag aattttcacc cacacgttcc aaacacttta tccggattga ttttactgat
      121 ggcagttcct ttgccaatat gtgtgattct gcttgtgatg aaatcattga attgattaag
      181 aaagagtttc cttcgattac atcccggaca aaataacaag tagaataatt aggaaaataa
      241 taattttttt tattatttaa taaatttaaa cttttttcaa agtactaggg gggtattatt
      301 tatgcttaaa tcaaaaatga ctaaagggat ctccttgtgc ctggcagtta tgatgctttt
      361 gcttggagtt ccactgaatg gatttgctgc tgtgactgac gagatttctg gtcagcaaga
      421 aggttctata ccggttgagg tatttacaca atcgaatgat atggagccgg aaaagaaatc
      481 ggaagggctt gatgattctt cagcttatag tagtgattcg gcaccgagcg gttatgttgg
      541 tgacaataca tttcttgatg taccgcagcg cagaatgatg ggtatggcaa aagcaaacgc
      601 aatagatacc caaaaaaaaa gttcttctca tagaagataa aaatccttgg gatagcaccg
      661 ctaatcaaat tgttttaagc ggcctgactg aatacaaaaa agtcacaaca gccggtttcc
      721 tgaatgaaga tttatcacag tacggtgtta ttgtttttgc aaatgaccaa ccttttgcaa
      781 cttatgaaaa ctatgcagaa tttaaagagt atttagaaat atttgcaagt attggtggag
      841 ttattgtctt tggtgcatgt gatgccggtt gggcaaatgg tcaattaaac gaagccattc
      901 caggcgatgt taccaagtct aatcattatg aatgttataa ctatattgct aatcccacgc
      961 atccgattgt taccggggca ttgacaaata acgattcatt gacggattct gaccttgctt
     1021 cctattactg tagtcatata tcgtttaatg aagagtcatt gcctcccggg acaactgtaa
     1081 ttcttaggga gacaagaacc aataaaccta cgttgataga atatcctctt ggaaagggac
     1141 gtgttattgc gtcgggtttg acttgggaac acaattacaa tcactggggt agaactatta
     1201 acggcgtgaa ggtcggcaat tttgccaagg caatggatga catgttttct tatgcgatta
     1261 gggtaagtag tattgatgtg aaagaattgc acttacttaa agaatggcgt cttaagaaaa
     1321 ttgcacactc aattaccgta gctgacggtt catctcaaag cggtgaattt ttaccgattg
     1381 ccggtgcaac agtgacaatc gacgggaaaa agtatgtaac tgatgaaaac ggtcaggtct
     1441 tttataatga gaattatgga cttcatacgg ccgttgtttc agcttcaggt tattacgaac
     1501 gcaaattaat gtataatatg caagaacgta catcccgtat cttctttttg gaaaaaaagg
     1561 ttaacgatgg actgccgtat attattcaga ctaatggtgt acgcaactat tccggaattt
     1621 atcttgatct gcgtgaccag gcattgcatt ataccgaaaa tagaactgat gtagctgctc
     1681 ttgttattga cggtaactgg aatggccatg gagccggcag gtatgtaatt tatcaagacc
     1741 ctgtggtggg tgaacccggt aagtatattt catcaactac cggagtattc acattttctc
     1801 ccggaatggt atttaaaccc aacaggcagg ttaagttgaa gatgatcgct ggcgacggaa
     1861 aagagtccag gccgattaat ttaaaacttg tcattgacag agcacctagt caatatggag
     1921 gaggaaataa cggcggagga aacggtggcg gaaataacgg cggaaataat aatgccggac
     1981 ttaaaacggg tgttgataaa tttgattgga ttgggaacca tcctgtgcaa tcggataacg
     2041 atatctttac taaaattttg acgaccgaca tgtctatttc cagtgaattg ataccgattg
     2101 agatatcatc tgaacgcgat gacgatggta ctactattta caaaggggtt ataggattta
     2161 tatccggatc ggcaaccaaa gatcttctta cgggcaaagg tgaaggtttt gctattgaat
     2221 caccttggga tgaacccaag aaacaaatca agggctacaa aactgccggt aaccccaagg
     2281 cgtattttaa taatctcaaa agaaagtacg gaaaagattg gaaaccatcg aagctgtctg
     2341 ccacatttga tgttgaagtc aatgcttgcg gttaccttga actgaaagta ggtcaaaatg
     2401 gaaatattat tagtagtgat ggtggtattg taattgacgc aagtggagat tttacaatag
     2461 ggcgtacttt tatggccggt ccggttccgg tttattttga aattaagccg ggaatcggct
     2521 tagaggtaaa cgccggtata gaattctata atgccgatgc cggtggactg ggttttagac
     2581 cgcattttgg cagtcttaca ttggaagttc cccatataac gcttgaaggg ggagtaggtg
     2641 ttcgtggtgt ggctaccgtt ggattgcaag gtagtggctc tcttaacata gagtttgaac
     2701 caggtaataa aactaatgga tcgcttgaat ttgggggagc aattcgtgta aaagtaatat
     2761 ttgtagttga ctataaatga gatttttgga atacaagtat taagttgtgg ccaaaagata
     2821 ataagatgta tagattattt gcagcgcgga gtctttcgtt tgaagaggcc gaattgtccc
     2881 ttgcatccag agattattta gatgactgta ctgcttggaa tggcggagaa aacaagtttt
     2941 tgaaattatc ggcgaattcc gctgatacga caataaaaac acttcaagaa ggtgttatgc
     3001 ccgacgcact tcctcaatta caaaaggtgg gagataaact tgtcatgctg tttttacgtg
     3061 acgtacccga acgcatgacg ggcaaccata ctcaattggt atattcggtg tttgacggcg
     3121 gtgtttggtc ggaaccgaag ccggtatggg acagcgaaac ggcagacttt ttctttgaaa
     3181 gtgtagttga tgacggcgaa ctatatgttg tttggcaaaa attaaagtca gttcctacaa
     3241 aagaaacacc ggaggattta ttagctgaag ttgcacaaaa ttctgagatc tgttttgcaa
     3301 agtggaatga cgaaacaaat gaatttacat ctcaatcata ccttacagat gacgatgaat
     3361 tggacatgta tcctaatgta gcggtaaggg atgatttagt gtcggttgtt tgggtgacca
     3421 atgatgaaaa tgatccgacc ggggagtccg ggaattacat aatcaagaga aaagatataa
     3481 taaatgatgc tatgggagat gtcagcgaac tttattctac aaccgaattt gttactgaga
     3541 tggctgcagg atactcggat gaagaactgc atatacttta tgctgttcta gaagaggatg
     3601 cggagaaatc caatatttat ttattaaaag ggggagctag tgaatccctc acctcgggaa
     3661 atgagtcagc atcactgcga tttgtaaaca atgtattcct ttggcagtca gaaggtgcta
     3721 tttaccggta cgacccaaca actcaagtac ttgcacaaat tattaccggt gaaaacggaa
     3781 gtgcgtcttc atcgtataaa tatgcaagca atggaacaaa gtccgcaata gtttgggtag
     3841 attctgaaaa gaatgccgat acaggtaaag acagttcagt gttaaaggca tcaatttttg
     3901 ccgatggaga atggagttca ccagttgttt tactgaataa tctcgataca aacatatcat
     3961 atatggatat agagctattg gaaaacggtg agtatgctgc tattttaaat acagcatctt
     4021 tttctgagga tcaagaggta aaaacatctc ttaaatttgc tgcgatttat cctcacaatg
     4081 atgttgaatt actatatgcg gcagtcgata aggcaaatgc acaaagcaat aagcaatcat
     4141 tgactatcta tctggccaat aacggcgatt cggtggttcc gtcgattgaa ttaagtgtag
     4201 atgctaacgg caataactat ttatacaaaa cattggatgt gtcgctaagc ccgggagagc
     4261 aaattgtatt aacggaggat atagatatat catctattaa cagtgtaaca gcagccgatg
     4321 taacggttaa tatcgatgaa gattctgaac cggacaataa cagagcacaa attactttag
     4381 ggcatgtgga cgtttcatta agcttagata cctatgacct tggagattct gttgtatttg
     4441 cattaacggc aagaaatgaa gcaaatacag tggcacattc tgctatcagt ataatagagg
     4501 attctcccga aggtatagtt ttagatgtaa aaaacattgg agaagttggc tgtgatgaca
     4561 gtgttcaata tttatatcaa atagacaaaa cgcaagtgca atttgatgaa ttcaattcta
     4621 agacctactt tttcaattta gaatcccttg aagaagattg gaacacagaa gataaccaac
     4681 tgttttatgt cgtctcccaa aatcctgatg aaacagatgt ggacccggaa gggataatgg
     4741 aagaggttaa aattgttcaa ccgacttcgg tagatatttc agtaaataag cttcattttg
     4801 attcggttga cagcgatagc gttcagctta gtgctgatat ttatccggca aattcttcaa
     4861 tcaataatat cctttggtca gttgaagatc cggatatcgt atatgttgat tcagaaggat
     4921 tggtaactcc gcttagaccc ggtactacaa ccattacatc aaaggtttcg gatgaagtat
     4981 atgatacaat aactgtgact gtagccgatc cttccacctt atataaaata tccgcaaagg
     5041 caggtacagg tggcacagtt tcaggagccg gtacctttgc atccgggaca gaagtaaaat
     5101 tgactgcaaa gccaaatacc aattattcat ttgaaggttg gtatgaagac ggcaaaaaaa
     5161 ttgatggaac gggtgctgcg tacacattca aagtttcaaa ggataggata cttgaagcgc
     5221 ggtttattta tgatgtatcc ggcggtggag gatatactgg cggaggtgat aaatctattt
     5281 cagctgcttt ggatgtcagc gagtttaagt tcgataaatc ttcacccacg gatattacgg
     5341 ttaagctttc ttcaggaagc tatacattcc gcgaaattaa gaacggaagt tatacactta
     5401 ttcctcacca ggattatatt gtgaacggaa atatgtttac tataaaagca gcatatttag
     5461 aaacccttga ggatggtcaa gccacactta catttactat gagcggagga aataacccgg
     5521 tgttgaccgt taccgttgag tccggagttt ccccggtaac cgaaggtgat ggagtgcttc
     5581 taaacggaaa agaaactaat ctcaaattta atccggatgg cagtgtcact ataaatgaag
     5641 aagacatcag cgaattatcc aaacccgtta ttcttacttt gccatacaaa gacggtggaa
     5701 attctcatgc gggcgttctt aaaaaagatg gacaggatgt tgtaatgcca ttctccgtat
     5761 acaaggacaa tacaatgatt atattgatat cggaaccggg gacttatggt gttgtaaaca
     5821 ataagaagaa ctttaacg
//
LOCUS       DAOOJC010000105         5835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_11128_length_5835_cov_4.226990, whole genome shotgun sequence.
ACCESSION   DAOOJC010000105 DAOOJC010000000
VERSION     DAOOJC010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11128_length_5835_cov_4.226990"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..910
                     /locus_tag="PLA01_04225"
     CDS             146..910
                     /locus_tag="PLA01_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HPD00539.1"
                     /translation="MKRMPGNWFYLLLILIILFTIGCMRLYYSKGASYAVIEKAFKAS
                     GANFVSSEFYVNAEVGDENQSFEDLSAMADDLAGDLNIEKNELYSRRLIENDSIDKIE
                     IIGTSEEGKRINICAQSERNADSRVKSYITVSVTVDSTKFEIEDIYGGLLKTLGKYKI
                     HPKVNTCITGYFEGKLDYAALNEVSKRVLEHAGAKKVNGITDSNLISVSAYSPGIDNT
                     ILENGKKVNMNLAIRYNSYEKRTYIWIAIPVITIEY"
     gene            979..2253
                     /gene="murA"
                     /locus_tag="PLA01_04230"
     CDS             979..2253
                     /gene="murA"
                     /locus_tag="PLA01_04230"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPD00540.1"
                     /translation="MPKLIVTEGKPLRGTVRVSGAKNSVLPIIAASILGDEESILEEI
                     PNLTDVNIMCDLLESFGVNVEFSENKSRLRIALKDIINTTAPYELVNKMRASILVMGP
                     LLARTGTAKISLPGGCAIGTRPVDLHLKGFAAMGAEINQGHGYIEARVNGRLRGSKIY
                     LDFPSVGATENIMMAAVLAEGQTIIENAAIEPEIVDLATYLTAMGADIKGAGTDTIKI
                     NGVHSLSGTNHAVIPDRIEAGTFMVAAAVTGGDVVVENIVPDHVKPITAKLREAGVEV
                     SEELSSIHVKAGTDIRPIDIKTHPYPGFPTDMQAQMTTLMTKANGTSMVIETIFENRF
                     MHLAELKRMGANVKIEGRSAIIEGKPKLTGAVVRATDLRAGAALILAGLIAEGVTEIT
                     EIEHVERGYVRIDQKLNALGANIKRVEDEVQE"
     gene            2692..3021
                     /locus_tag="PLA01_04235"
     CDS             2692..3021
                     /locus_tag="PLA01_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00541.1"
                     /translation="MKKLLGYFLLMIMIIVILPLIIVRGCSTVITDIVPEDDKREEQK
                     QEQKKEQTQAGKIKLKVYIKDKGTVEEMPLEEYIKGVWRLRCLLILSRKPLRHRRWLP
                     VHTHTGE"
     gene            2946..3755
                     /gene="spoIID"
                     /locus_tag="PLA01_04240"
     CDS             2946..3755
                     /gene="spoIID"
                     /locus_tag="PLA01_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HPD00542.1"
                     /translation="MPVDFEPEALKAQAVAARTYAYGRMKKIYTPSDDIHAGAHICTD
                     SGHCQAWIKKEDALNKWESSKASDNWKKIEKAVLDTEGQILLYENSVVNPVFHSNSGG
                     MTENSEDVWEGTVVPYLKSVVSEGEEASAGYEVETEFTKEDIVQKLKAQYPDIKVGKE
                     DLFDEMEVLERTDAGRVKSIKIGDKTLKGTEFRSLLGLRSTNFIIEDVDDSKLKITTK
                     GYGHGVGMSQWGANHRAKQGDDYEEILKYYYQGVYIDSIENMELILGTKEE"
     gene            3821..4732
                     /locus_tag="PLA01_04245"
     CDS             3821..4732
                     /locus_tag="PLA01_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPD00543.1"
                     /translation="MDFKKLFPDKKVTQKKILDFFDKKGFYIVLVLCITIIGVTAALV
                     TTHNITSSKNYGNDNIISPDMASGIMDEDESSTYLLEDEFDIGNAEEAAAKTSTETGE
                     PSDAAASPTPKPTEEPKKTELPKEQKATEPPKDEKKPEANTKNKTEAANAASNSENAK
                     QTFAMPVFGKVTLEFAMDKLVYSKTLEEWRAHSGVDLRADRGTPVKVVADGAVTEVKN
                     DPRFGVTVIVEHSNGIKTLYANLANADMVTPNQKVKQGEVIGSVGNTAVIESAEPPHL
                     HFEVIKDGKPVDPGAYLSLSSEILNDN"
     gene            complement(4900..5658)
                     /locus_tag="PLA01_04250"
     CDS             complement(4900..5658)
                     /locus_tag="PLA01_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00544.1"
                     /translation="MAIEKIDETFISYASDILADTNQGLTGTQIVKYCNIYAVDFGVK
                     IPITSPDFGKFGSIVPNKRTALYKNLREFNGQQQFVIIKELCELPQFESNSRVQELKK
                     RLIERFSRFSLSPLYLEKYQTTGWERVDRALEEMKLRLDTADNEEKFQAIGMIGRETL
                     ITIAQQVFDSEKHPTLDGVEASKTDVKRMLEAYLKIELAENSEKVIKFTKSAVDLANQ
                     LTHDRGATKRDASICLISVTAVASLIKSIQLTGK"
BASE COUNT         1936 a          999 c         1364 g         1536 t
ORIGIN      
        1 aaacagccga tttaaaacca tttaaattta aagcaaatct ttaagaaata ttggatgtat
       61 atcccattgc attaagggtt ataattctac tataactgtg cccttttcct acaagcaggc
      121 acataacggt agaaggggat tatacatgaa aagaatgccg ggaaattggt tttatttgct
      181 tttaatatta ataatactat ttacaatagg gtgtatgagg ctttattata gtaagggggc
      241 ttcttatgct gtcattgaaa aagcatttaa agcttccgga gctaattttg tcagcagcga
      301 attttacgtc aatgctgaag tgggggatga gaaccaaagc tttgaggatc tttccgcaat
      361 ggcggatgac cttgcggggg atttgaatat tgaaaaaaac gagttgtatt caagaagact
      421 tatagagaat gactctattg acaaaataga gataattgga acttctgaag aaggaaaaag
      481 aataaatatc tgtgcacaat cggaaagaaa cgccgatagc agagttaaat cctacataac
      541 ggtaagtgtt actgtggatt ctacgaagtt tgaaattgag gatatatatg gaggcttact
      601 caaaacctta ggcaaataca aaatccatcc aaaggtaaat acctgtatta cagggtattt
      661 tgaggggaaa ctggattatg ccgcattgaa tgaagtaagc aaacgggtac ttgaacacgc
      721 gggtgcgaaa aaggtaaacg gtataacgga tagtaatttg ataagtgttt cggcttattc
      781 tcccggtata gacaacacta tactggaaaa cgggaaaaag gttaacatga atctggcaat
      841 aagatataat tcatacgaga aaagaactta tatctggatt gcaataccgg ttataaccat
      901 tgagtactga gaaaatgtcc gtgaaaaagt acatacaaaa gacaaaaaac aataaaaaaa
      961 acgaaaggaa gaatcgatgt gcctaaacta attgtaaccg aaggaaagcc cttgagggga
     1021 acggtaagag taagcggtgc aaagaattcg gtgttgccca ttattgctgc ctctatttta
     1081 ggtgacgagg aaagcattct tgaagagata ccaaatctta ccgatgttaa tatcatgtgt
     1141 gatttgttgg agtcttttgg cgtaaatgtt gagttttcag aaaacaaatc cagacttcgc
     1201 attgctttga aggatataat aaatacaact gcaccctatg agctggttaa taaaatgagg
     1261 gcatctattc ttgttatggg acctctttta gccagaacgg gcacagccaa gatttcactc
     1321 cccggaggtt gtgcaatagg tacccggccg gtggatctgc accttaaagg ctttgctgca
     1381 atgggggcag aaataaatca gggacatggc tatatagaag caagagtaaa cggaagactt
     1441 agaggaagta aaatatatct ggattttccc agtgtcggtg ccaccgaaaa tattatgatg
     1501 gcagcagtat tggcagaggg gcagaccata attgaaaatg ctgcgataga gccggagatt
     1561 gtagaccttg ccacatattt gacagccatg ggagcggaca tcaagggagc agggaccgat
     1621 acgataaaga taaacggtgt tcatagttta agcggtacaa atcatgctgt gattccggac
     1681 aggattgagg caggaacctt tatggtggct gcggcggtaa ccggtggaga tgtggtggtg
     1741 gaaaacatcg tgccggatca tgtaaagcct ataacggcga agcttagaga agcaggagtt
     1801 gaggtttctg aagagctttc gagcatacat gtaaaggcag gcactgatat acggcctatt
     1861 gatatcaaga cccatcctta tccgggtttc ccaacggata tgcaggcaca gatgaccact
     1921 ttgatgacca aggccaacgg aaccagcatg gttattgaaa caatttttga aaacaggttt
     1981 atgcatctgg cggagctcaa aagaatgggt gccaatgtta aaattgaagg aagaagtgca
     2041 attattgagg gtaagcccaa gcttaccgga gcggtggtga gggctacgga tttgagggcc
     2101 ggtgccgcac ttattctggc cggacttata gctgagggtg ttacagagat tacggagata
     2161 gagcatgtag aaagaggata tgtgagaatc gaccaaaagc tcaacgccct aggtgcaaat
     2221 ataaaaagag ttgaggatga agtgcaggaa taacacagag gaatcagggg gatcacaggg
     2281 ggacacaggg ggacggttct tccgtgttaa aatagcatgt acatattaaa ggacgcagat
     2341 gatgcgtcct ttaattatga taatttcacg ggtaaagggg agggacaaaa tggatagagt
     2401 aatcagtaag tactatgttg ggttttaagg cgaaccggaa attcgcttga ttcggggtga
     2461 agaaaatgta ttttagacaa agatgcacaa aatgttttaa gcgaaatgtt ggaatattta
     2521 atagatgcca agtccggtaa caatactgct tggatagctt atgagtaagt tcctgtgacc
     2581 atttttcatt atctaaaaaa gaataaccga aagcttgcaa tcatatctat ttattaataa
     2641 caatacaatc gggataaaaa actactaaaa tcacaggaaa aggtacggac aatgaaaaag
     2701 cttttgggtt attttctttt aatgataatg ataattgtta tacttccgct gattattgtc
     2761 aggggctgca gtacggttat aaccgatatt gttcccgagg atgataagag ggaagaacaa
     2821 aagcaggaac aaaagaaaga gcaaacgcag gcaggaaaaa taaagctgaa ggtatatata
     2881 aaggataagg gtacagttga ggaaatgcct ttggaagaat atataaaggg tgtgtggcgg
     2941 ctgagatgcc tgttgatttt gagccggaag cccttaaggc acaggcggtg gctgcccgta
     3001 catacgcata cgggagaatg aaaaaaatat acactcccag cgacgatata catgccggtg
     3061 ctcatatatg taccgattca ggtcattgtc aggcatggat taaaaaagag gatgccctca
     3121 acaagtggga atcctccaaa gcctcggata attggaaaaa gattgagaag gctgtcttgg
     3181 atacggaggg acagattctc ttatatgaaa acagtgtcgt aaaccccgta ttccattcga
     3241 acagcggcgg gatgaccgag aattcggagg acgtatggga gggaacggtc gttccttatc
     3301 tcaaaagtgt tgtaagtgaa ggtgaggaag ccagtgcggg atatgaggtt gaaacggaat
     3361 ttacaaaaga ggatatagtt caaaaattaa aggcacaata tccggatatc aaagtaggca
     3421 aagaggacct ttttgatgaa atggaggttc ttgaacggac cgatgcggga agagtgaaaa
     3481 gcattaaaat aggagacaag acccttaagg gcacagagtt tagaagtctt ttagggctca
     3541 ggtcaacaaa tttcatcata gaagatgtgg atgactccaa gctcaaaata accaccaaag
     3601 gatacgggca cggtgtggga atgagtcaat ggggagcaaa ccaccgggct aaacagggag
     3661 atgactatga ggaaattcta aaatactatt atcagggcgt atatattgat tcgattgaga
     3721 atatggagct tatattgggt actaaagagg aataaatatt ttttacctct atgtatattg
     3781 ttgccatagg cggaaacaat aatatatgga ggtggaaaaa gtggatttta aaaaattgtt
     3841 tccagataag aaggttacgc aaaaaaagat tttggatttt tttgataaaa aaggatttta
     3901 tattgtttta gtattgtgta taaccattat tggagtcaca gcagcacttg ttacaaccca
     3961 taatattact tcatccaaga actacggcaa cgataatatc atttcaccgg atatggcaag
     4021 cggcataatg gatgaagacg aaagcagtac ttacttattg gaggatgagt ttgacattgg
     4081 taatgcagaa gaggcagcgg caaaaacatc aacggaaacc ggcgagccct ctgatgctgc
     4141 agcaagtcca actcccaaac cgacagaaga accgaagaag acggagttac ccaaagagca
     4201 aaaggctact gagccaccaa aggatgagaa aaagccggag gctaatacta aaaataagac
     4261 tgaggcagca aacgctgcaa gcaatagcga aaatgcaaaa cagacctttg caatgccggt
     4321 atttggaaaa gtgacccttg aatttgcaat ggacaagctg gtttattcaa agacattgga
     4381 ggaatggaga gcacacagcg gcgtagattt aagagcagac aggggaacac cggtaaaggt
     4441 tgttgccgac ggtgcggtaa ccgaggtcaa aaatgatcca aggtttggtg tgaccgtaat
     4501 agtggagcac tcaaacggta ttaagacttt atacgcaaac cttgccaatg cagatatggt
     4561 tactccgaac cagaaggtta agcagggaga agtaatagga agtgtcggaa acacagcggt
     4621 aattgagtcg gctgagcctc cgcacctgca ctttgaagtg ataaaggacg gcaaaccggt
     4681 ggatcccggt gcttacctct ccttatcttc agagatatta aacgataatt aataagctat
     4741 attgaaaaag gaagctgcct taaagaagcg ttcacttagg gattgaagaa aaacttaact
     4801 gaacagcttc aagcggggca aaaaagacca gtcacaatca agcgtatgtt cttgaagtga
     4861 ctggcctttt ctgttatatt tttgtctcta ggttatctat tattttccag ttaactgtat
     4921 tgatttaatg agagatgcta ctgctgtaac agatattaaa caaattgacg catctcgttt
     4981 tgtagcccct ctgtcatgag taagttgatt cgctaaatct accgctgatt ttgtaaactt
     5041 tatcactttt tctgagttct ctgcaagttc aattttcagg taggcttcaa gcattcgctt
     5101 tacatcagtt ttacttgctt caactccatc caatgttgga tgcttttcag aatcaaatac
     5161 ctgttgtgca attgttataa gtgtttctcg tccaatcata ccaattgctt gaaacttttc
     5221 ttcattgtca gctgtatcga gtcgcaattt catttcttca agcgctcgat cgacacgttc
     5281 ccatcctgta gtctgatact tttcaagata tagtggactt aatgaaaacc gtgaaaaacg
     5341 ctcaatcagc ctctttttta attcttgaac acggctattg ctttcaaatt gtggcaactc
     5401 acatagttct ttgataatga caaattgttg ctgaccattg aactcgcgta agtttttata
     5461 taatgctgtg cgtttatttg gaactatact accgaatttt ccgaaatcag ggcttgtaat
     5521 agggatttta acaccaaaat ccacagcata aatattacaa tatttcacaa tctgtgtccc
     5581 agttaaaccc tgattagtat ctgccaatat atctgaagca tatgaaataa aagtctcatc
     5641 aattttttct attgccatag ttatcctcct aatgctcttt acgaataaaa attaatccgg
     5701 tcttctacca catacaccct tcttacacca accctaaata cttctcaaag aataacatta
     5761 gtttttcgat aattccttgc tttttcgcgg ctctgcctcc gccaaagcga gatacaggtg
     5821 gcatgatttt atcaa
//
LOCUS       DAOOJC010000106         5822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_11152_length_5822_cov_3.694122, whole genome shotgun sequence.
ACCESSION   DAOOJC010000106 DAOOJC010000000
VERSION     DAOOJC010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11152_length_5822_cov_3.694122"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLA01_04255"
     CDS             <1..222
                     /locus_tag="PLA01_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPD00545.1"
                     /translation="GVPGWIVQVDDSKVSPMVKQMVSMTQDSTAWILWWDVFLEGEDA
                     EIHKNLVVELIAGQISPEDFAKEMQKLNE"
     gene            complement(295..1053)
                     /locus_tag="PLA01_04260"
     CDS             complement(295..1053)
                     /locus_tag="PLA01_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPD00546.1"
                     /translation="MPRKPRVKSSTDIYHVMLRGINGQVIFHDNKDYEKLIQTIKELK
                     EISKYEVYAYCLMTNHVHLLIKERNEDLGIVFRRIGAKYVYWYNRKYKRRGYLFQDRY
                     KSEAVESDEYLLTVLRYIHQNPVKAGIAKNVHSYPWSSYNEYITKSSLCDTDYITSLF
                     AEDKSKAILLFQQFNAQENNDKCLEYEEPQKIYDDEAIQMIIEISGVKEPHEIQILEP
                     DKRVKVVKELRSIGLSMRQIERLTGLSISTIRRL"
     gene            1595..4642
                     /locus_tag="PLA01_04265"
     CDS             1595..4642
                     /locus_tag="PLA01_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPD00547.1"
                     /translation="MRKKAILSMFLVLSLIMISIPKLQYPVMAALAELKGTDIYNGLR
                     GQSFNSGWKFNKGDVNNGQSIGYNDSNWLDVTLPHDWSIFNSFNQWSPAGDGGGYLDG
                     GIGWYRKTFTIPSDYSGKRVFIVFDGAYMNSQVWINGTLLGSRPYGYSSFQYDLTPYL
                     NMGGSNVIAVRLNNNQPTSRWYSGSGIYRNVWLTVLDPVHVDYCGMSITTPKVSSDSA
                     IASVSTKVLNQGNSAKTVSVKTTIMDANGNQVASDSSTAINISAGGTNTFSQNLTVTN
                     PYLWSPDSPYLYMVQTQVVVNGDVVDTYKSTMGFRNLNFSSTTGFSLNGVRIKIKGVC
                     LHHDLGALGAAVNYRAIERQLEIMKEMGCNAIRTSHNPPDPQVLEICDRLGLMVMDEA
                     FDCWETGKTTNDYHLYFKDWAQRDLQDMVKRDRNHPSIIMWSVGNEIPSASVETATKL
                     KNWVREIDTTRPVTWGCFAINMGEETYKKVASVLDLVGYNYFPFTYDGGHKEHPEWIM
                     FGSETSSAVRSRGVYKTPTNENILTGNDNQCSSYDNSVVAWGNSAESSYFEINKRDYM
                     LGEFVWTGFDYIGEPTPYKWPSKSSYFGIVDTCGFPKDIYYFYQSKWSDKPMVHILPH
                     WNWSNGKTIEVWAYSNCDSVELFLNGTSLGVKSMGYNGHVSWNVPWSPGTLRAKAVKG
                     GTVVYDEVVTAGNPAKIQLKPDRTSITADGKDLVFIETDIVDSNNVLVPTASNTVNFS
                     ISGPGVIVGVDNGNAASVEPYKANSRQAFSGKCLVIVQATNNSGTITVTANSNGLASD
                     SVTIEATGGTPEPTPVPRSAFTQIEAENYSSQSGIQTEDCSEGGTNVGFIENGDYVAY
                     KAVDFGNGAASFKARVASATSGGNIELRLDGINGPLVGTCTVTGTGGWQEWDYATCEV
                     TDLSGIHDLYLKFAGGEGYLFNINWFTFEEGNNTGLLGDVNLDGSIDSTDLQLVKRHI
                     LNKKPLEGIGLINADVNRDKSVDSTDVTLFKRYILRKIKSFDF"
     gene            4671..>5822
                     /locus_tag="PLA01_04270"
     CDS             4671..>5822
                     /locus_tag="PLA01_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HPD00548.1"
                     /translation="MRKIVLILLSILLIMTFNIYSCFADNPIVQTIYTADPAPMVYDG
                     RCYVYTTHDEDVIIDNFFTMNDWRCYSSEDMVNWTDHGCVLSFTDFSWSSGKAWAGQC
                     VERNGKFYFYVPLAKKSGGEAIGVAVSDSPTGPFVDALGKPLIDRGWGDIDPTVFIDD
                     DGQAYLYWGNPDLYYVKLNPDMISYSGGIVKVPLTTSGFGVRSKDDRPTTYEEGPWFY
                     KRNNLYYMVFAAGPISEHIGYSTSTSPTGPWTYRGVVMPTQGSSFTNHPGVIDYKGNS
                     YFFYHNGALPGGGGYHRSVCVEQFEYNLDGTIPKINMTKEGPAQIGTLNPYIRNEAET
                     ICWSSGIETEKCSEGGMNVGFIENGDYIKVKGVNFGSGAASFEVRVASAS"
BASE COUNT         1739 a         1075 c         1334 g         1674 t
ORIGIN      
        1 ggtgtgccgg gctggattgt gcaggtggac gattcaaaag tatcaccgat ggttaagcaa
       61 atggtgagta tgacacagga ttcaacggca tggattcttt ggtgggatgt gttcctggaa
      121 ggtgaagatg ccgaaataca taagaatctt gtggttgaat tgatcgccgg acaaatatca
      181 ccagaggatt ttgcaaagga aatgcaaaaa ctcaacgaat aacacgggag tatgacacga
      241 ggcgatgaca cggggggacg gtttttccga acacgagggg acggttctta cgtgtcataa
      301 tctccggatt gtactaatgc ttaaacctgt gagcctttct atttgtctca ttgaaagacc
      361 tatacttctc agttctttaa caactttaac tcttttatcc ggttcaagta tttggatttc
      421 gtgaggttct tttactccgc ttatttcgat tatcatttga atagcctcat cgtcatatat
      481 tttttgaggc tcttcatatt caaggcactt atcattgttt tcctgagcat tgaattgttg
      541 aaataacaaa attgctttgc ttttatcctc tgcgaaaaga ctggttatat aatctgtgtc
      601 acaaagactg ctttttgtaa tgtattcatt ataactactc cacggatagc tgtgtacatt
      661 tttggcaata cctgctttaa ccggattctg atgaatatat ctcaatactg ttaataaata
      721 ctcatcgctt tcaacagctt cgcttttata tctatcctga aaaaggtacc ctcttctctt
      781 atatttacgg ttgtaccaat aaacatattt ggcaccgatt cttctgaata ctattcctaa
      841 atcctcattc cgttccttta tgagcagatg tacgtggttt gtcataaggc aatatgcata
      901 gacttcgtat ttgcttattt cttttaattc tttaattgtt tgaattaatt tttcatagtc
      961 tttattatca tggaaaatga cttgaccgtt tattcctctg agcatcacat gataaatatc
     1021 ggtgctgctt tttactctcg gttttcttgg cataacaatc acctcaaata aaatatatct
     1081 gaaaaaaaag atacctttga cacggaagaa ccgtccccct gtgttaattt aaacttttct
     1141 gaaacggttt gctatacttt aacatagcga tattacctga aatcaaatag atgatatcac
     1201 ttgaattata atcccgtgca aatctcaata tatgcatgaa aataataaca taaaaggtta
     1261 aatactatag gctaattgta ccatagtatg taaatgatat tcaataaaac acaacaaaaa
     1321 aatatataac tcgtacaaag cgattaatta taaaattccg ttaaaattcc caacttaaaa
     1381 tggctagtat tttcgtaaaa gtaacacaag gggcaatcaa attaatttat atacatggat
     1441 aaagagctca gttaatccca gggtattttg tgctttcaaa ataagaatca atgttattta
     1501 agaattctat tcctgtgtcc cctgagaagt tgtgattaga acaataaatt tattaatttt
     1561 atctataaat tatttatatt aaggaggagg tagtatgaga aaaaaagcaa ttctaagcat
     1621 gttcttagta ttgtcactaa ttatgatcag cattccaaaa ctacaatatc cggtaatggc
     1681 tgctttggca gaattaaaag gaacggatat ttacaacgga ttgagaggtc aaagttttaa
     1741 ttcaggctgg aagtttaata agggagatgt gaacaatggc caaagtatag gctataatga
     1801 cagcaattgg ctggatgtca ctctgcctca cgattggagt atttttaatt cctttaacca
     1861 atggtcccct gcgggagacg gaggggggta tcttgacgga ggaatcgggt ggtacagaaa
     1921 aacctttaca ataccctcgg attattccgg caagagagtt ttcatagtat tcgacggggc
     1981 ttatatgaac agtcaggtat ggataaacgg tacattgctg gggagccgtc cgtatgggta
     2041 tagttccttt caatatgact tgactcctta cctcaatatg ggcggaagta acgtaattgc
     2101 agtcagactc aacaacaacc agcctacaag ccgatggtat tcgggaagcg gtatttatcg
     2161 taacgtatgg ctgacagttt tagacccggt tcatgtggat tattgcggaa tgtctataac
     2221 gactccaaag gtgagcagcg attctgctat agccagtgtc agcacaaagg ttttgaacca
     2281 gggcaattca gcaaaaacgg tttctgtgaa aacaacgatt atggatgcaa atggcaacca
     2341 ggttgcatcc gattcatcaa cagcaattaa tatatcggcc ggcggtacca atacattcag
     2401 ccagaacctc acggtaacca atccctattt atggtctccc gattccccgt atctttatat
     2461 ggttcaaacg caggtggttg taaatggaga cgtggtggat acctataagt cgaccatggg
     2521 attccgtaat cttaacttca gcagtaccac cggtttttct ttaaacggtg tcagaataaa
     2581 aataaaaggt gtgtgccttc atcatgactt gggtgcttta ggtgcggcag ttaactaccg
     2641 tgccattgaa agacagcttg agataatgaa agaaatggga tgtaatgcta tccgtacctc
     2701 acataatcct cccgatcccc aagtattgga gatatgtgac agattgggcc tgatggttat
     2761 ggatgaagcc tttgattgct gggaaaccgg aaaaacaacc aatgactatc atctgtattt
     2821 caaagactgg gcacaaaggg atcttcagga tatggttaaa agagaccgca accatccctc
     2881 gattattatg tggagtgtag gtaatgaaat tccctcggct tcagttgaaa ctgccactaa
     2941 gcttaaaaac tgggtaaggg aaattgatac cacccgtccg gtaacatggg gatgttttgc
     3001 aataaatatg ggagaggaaa cttataaaaa ggttgccagt gttcttgatt tggtcggata
     3061 caattatttt ccctttacgt atgacggagg acacaaggaa catcccgaat ggataatgtt
     3121 cggcagtgaa acaagctcgg cggtaagaag ccgtggtgtc tataaaactc ctaccaatga
     3181 aaatatactg accggcaatg acaaccaatg ctcttcttat gacaacagtg tagttgcctg
     3241 gggtaacagt gcagaatcgt catattttga aatcaataag cgggattaca tgttagggga
     3301 atttgtttgg acagggtttg actatatcgg tgaaccgaca ccttacaagt ggccttctaa
     3361 aagttcgtat ttcggaattg ttgacacatg tggtttcccc aaagatatct attatttcta
     3421 tcagagcaaa tggagtgaca agccaatggt gcatattctg cctcattgga actggtcgaa
     3481 cggaaaaacc atagaagttt gggcttatag caactgcgat tcggtggagc ttttcttaaa
     3541 cggtacttcc cttggggtaa agagtatggg atataacggc cacgtttcgt ggaatgttcc
     3601 ttggtctccg ggaacactga gagccaaagc tgtaaaaggc ggaacagtag tttatgacga
     3661 ggttgttaca gccggaaatc ccgcaaaaat tcagttaaaa ccggatcgga cgtctattac
     3721 ggccgatggc aaggatttgg tatttataga aaccgatatc gtggacagta acaatgttct
     3781 cgttccaaca gcaagcaata ctgtgaattt ctccatatcc ggacccggag taattgtcgg
     3841 agtcgacaat ggaaatgctg ctagtgtgga accttacaag gcaaacagca ggcaggcttt
     3901 tagcggcaag tgccttgtga ttgttcaggc aactaataac agtgggacta tcacggtaac
     3961 agccaactca aacggattgg catccgacag tgtgactatt gaggcaaccg gaggaacacc
     4021 tgaacctact cctgttccaa ggtctgcgtt tacacaaatc gaagcggaaa actacagctc
     4081 tcaatcggga atacaaactg aagactgcag cgaaggcggg acgaatgtag gctttattga
     4141 aaacggtgat tatgttgctt acaaggctgt tgatttcggc aacggagcgg caagtttcaa
     4201 ggccagagta gcaagtgcca caagtggagg taatattgag cttaggctgg atggcatcaa
     4261 cggtcctttg gtgggtacct gtacggttac cggcactgga ggttggcagg agtgggatta
     4321 tgcaacatgt gaggttacag acctgagcgg gatccatgac ctgtatttga aatttgcagg
     4381 tggtgaaggt tatttattta atattaactg gtttacattt gaagaaggaa ataataccgg
     4441 gcttttgggt gatgtaaacc ttgacggaag tattgactcc acagaccttc aattggtaaa
     4501 aaggcatata ctcaataaaa aaccgcttga aggaatcggt cttataaatg cagatgtaaa
     4561 cagggacaaa agtgtggact ctaccgatgt tacattattt aagagatata tattgagaaa
     4621 aataaagtct tttgattttt gagtccaatt taaggtgggg gtattgaaaa atgagaaaga
     4681 ttgttttaat actgttgtca atccttttga tcatgacttt taatatttat tcatgttttg
     4741 cggataaccc gatagtccag acaatctata ctgccgatcc cgctccgatg gtatatgacg
     4801 ggagatgtta cgtgtatacc acccacgacg aggatgttat tatagataac ttctttacca
     4861 tgaatgactg gaggtgctac tcctcagaag acatggtaaa ttggaccgat catggttgtg
     4921 tgctgtcctt cactgatttc agttggtcaa gcggtaaggc atgggcagga caatgtgtgg
     4981 aaagaaatgg caaattttat ttctatgttc ccttggcaaa gaaaagcggc ggagaggcga
     5041 ttggtgttgc agtatcggac agccccacag gcccgtttgt cgatgctttg ggcaagccct
     5101 tgatagaccg ggggtggggt gatatagacc ctaccgtgtt tattgatgat gacggacagg
     5161 cttacctata ctggggaaac cccgatcttt actatgtgaa gctaaatccg gacatgattt
     5221 cgtactcggg cggtattgtc aaagtgcctc ttaccacatc aggcttcgga gtgcgaagta
     5281 aagacgacag accgactacc tatgaggaag gcccgtggtt ttacaagcga aataatttat
     5341 attatatggt gttcgcagca ggtccgatat ccgaacatat tggatattca acgagcacga
     5401 gtcctactgg gccgtggacc tatcgcggcg ttgtaatgcc tactcaggga agcagcttca
     5461 ccaatcatcc cggggtaatt gattataaag ggaattccta cttcttctac cataatggtg
     5521 ctttgccggg aggaggaggc taccaccgtt ccgtttgtgt ggaacagttt gaatataatt
     5581 tagacggaac aattcccaag attaatatga ctaaagaagg gcctgcacag ataggcactt
     5641 tgaacccata tataagaaac gaagctgaaa ccatttgctg gagttcgggg atagagacgg
     5701 aaaaatgcag tgaaggcgga atgaatgtag gctttattga aaatggggat tacataaagg
     5761 ttaagggagt gaatttcggt tccggtgcgg catcctttga ggtgagagtg gcatcggcat
     5821 ca
//
LOCUS       DAOOJC010000107         5809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_11181_length_5809_cov_3.791449, whole genome shotgun sequence.
ACCESSION   DAOOJC010000107 DAOOJC010000000
VERSION     DAOOJC010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11181_length_5809_cov_3.791449"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..2385)
                     /locus_tag="PLA01_04275"
     CDS             complement(58..2385)
                     /locus_tag="PLA01_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPD00549.1"
                     /translation="MTSDSHPGCSVTVWASRPSGSEYPIGRTTVTITATDECGNQAVK
                     TFDVIVELSIIQIEVIKEIEPNITAIMVKYGTERENIPLPEKVTAVYHDGTRKEVGVT
                     WDDGTPLYNKNRPVTYKFTGELNIDPFREANPKNLKYQLYVTVFEPFYTLLDPIAAAY
                     RLDSPADIKTYISADRVSEVIDVVCGGDSLRQNIDYTVTYDDNKRMKVLIIKKDYLSR
                     FLQRDGDFVQFKVTFWGGLSDRLMVTAVEGYKPGDDATLQSLYIGGKPVSGFIRNKYN
                     YTENVAYGVESTRVAAVTWDVNARVVVNGKVLRSHELSEAIPLNVGSNTIEIDVIAQN
                     GTSTNRYTVTVNRAAPPSTNGNDGKDNDKTPDRQPQAVELPPVEIPTVEIPSATLGGL
                     KIIATVDAATGTVKAEFDTETLNNALEGTLTGDDGKKTLGVGLPKSDTESYNVELPAI
                     ALSNLQHGINIEITTGAAKITLPDNMIAGTDIENRGNAGVTIKNVDKSTLPEEVRNSV
                     GDKPVIQLVFTVDGEQKAWNNPNAPVTVSIPYTPTEEELNDPEHIVVWYIDGSGKVVP
                     VPNGHYDPVTGTVTFTTTHFSYFAVAYVRKTFGDLGSVEWARKPIEVMASKGIISGTG
                     LDAFSPTRSITRADYLVLLVKTLGLTTEFDNNFDDVSPDANYYEALGIAKKLGIAVGI
                     GNNRFNPEETISRQDMMVLTVRALEKAGKLQCSGVNELPDKFSDKGEIAGYAVDSVSA
                     LVKEGLIVGSDNRLNPRANTTRAEAAVFLYRIFNR"
     gene            complement(2375..4819)
                     /locus_tag="PLA01_04280"
     CDS             complement(2375..4819)
                     /locus_tag="PLA01_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HPD00550.1"
                     /translation="MRIYMSEKYFHAERFVIPKGCTEGGVRIEKNNSRKRKIELLKKI
                     VVLSLVMIMAWSMVVLPPKVQAVGINSLNIVNYGLNEMGNDASLLGDTDEIIDSTSDD
                     VYGGRVPLVDSVPDMVKSDVEKTTWVWGEDERDDERNNEYTVNEDRSQGPPRLASLRV
                     TNGQFFPAFDINFEIYTMDVPPEVTSVEITAIAVNPDDEVYVGTDTIGAGYRGRRTKT
                     EALICGRNEIPVTVMNKYNEAVVYTLIVNRAESEFDRLKNLKIWVDNEEVELSPAFDP
                     NVRVYSVNLGQGQDLKPVKFTAEPENPSSRVVLGWNPSQTQTDDLTESEGWTSKTKML
                     NPEEEHTIFFTVKPEESNNVGAYLVMINGTDSNNDLESLKVFTSDRKQEFALSQNFDP
                     DVTEYSIEVPEETKTICLEADASSPMAGVLVNGQQYDYYPVPLDYGTNHIKVTVVAPN
                     LSTKDYVLRIERKIPDELVQRLDSIRFYYEGQDIDFEPAFSNDCDSYKVNVPYKINGH
                     VTYSIIFEVTKPLSATDMQMRIKDKNSLKDADLTYLDHSTAYAVLDVNRVWVLYLETE
                     NGPQRRIEFYIKHEISKEAMLRDGDGLRLYASDCDPSENLLEGLYQPDTTEYTVSVKN
                     QVDKVTVSAIPNDEGAIISIRGEKGRNCEVPLEEGENIIVVYVMAYDSLTYLTYTITV
                     IRRPPDTNPPVLFLPEDMTVEPNLPDGAVISLPIYAYDEEDGYVPVTATADGVPISDI
                     NNAFFPIKTTEVKVEAEDSSGNRAEGTFYITVKHTTPPTIRVYDKRNDLEIGDSIVFA
                     TNLSDNVVLDLLYRDI"
     gene            complement(4934..>5809)
                     /locus_tag="PLA01_04285"
     CDS             complement(4934..>5809)
                     /locus_tag="PLA01_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00551.1"
                     /translation="KKSIEIGERYGTLNIPMVCYGELAAVFLREGDEARAEECTRRCL
                     TLSQQYGHRYYFQLKTHFGEVLKFALKRGISPEFVREMLVYGGYTTQRVYINTMGTFY
                     IAPAHDRCSCVKIRTQKSRELLAYLLEHREGVSRDRIFSDLWHDSEGDVTGMFHTRRG
                     EIRRAFESLGAKNPIVYKKGIYKLDMEEIVCDYDAFRKVACEFRKNPSPQNAQKVVDH
                     YTGRYLDDMEALWAESTRLICEDTFLQAAETLMESYRESGERIKIMELLHRCTGLGYQ
                     GHRVDAAKEGKKNKI"
BASE COUNT         1426 a         1465 c         1158 g         1760 t
ORIGIN      
        1 ctatgccgga tttaatataa gatgccccca ttgcttttgt ttcatttttc atggctgtta
       61 tctgttgaat atcctgtata ggaaaaccgc tgcttcagcc cgtgtggtat tggcacgagg
      121 gttcagccta ttatcggaac cgacaatcaa gccttccttg accagagcgg acacgctgtc
      181 caccgcgtag cctgctattt cacccttatc gctgaattta tccggcagct cgttaacacc
      241 cgaacattgc agcttcccgg ctttctccag tgctctgacc gtcagcacca tcatgtcctg
      301 acgggaaatg gtttcttcag ggttgaaccg gttgttgcct atgcctacgg caatgccaag
      361 ttttttagcg atgccgagag cttcataata gttggcatcc ggtgatacat cgtcaaagtt
      421 attgtcaaat tccgttgtga gccctaaagt cttgacaagc agtaccagat aatcggccct
      481 ggtgatactt ctggtgggag agaaggcatc caaccctgtt ccgctgatga tgcccttgga
      541 ggccataacc tcaatgggtt ttcttgccca ttccacactg cccaggtcac caaatgtttt
      601 acgcacgtat gccacggcaa aatagctgaa atgggtggtt gtaaaggtaa ctgttccggt
      661 taccggatcg tagtgcccgt tgggtacggg aaccaccttg ccgctgccgt caatgtacca
      721 aacgacaata tgctccggat cattcaactc ttcctctgtc ggtgtgtagg gtatcgatac
      781 cgtcaccggt gcattcgggt tgttccaagc tttctgctcg ccatcaaccg taaagaccag
      841 ttgaataacc ggcttatctc ctactgaatt tcgaacttcc tccggaaggg ttgatttatc
      901 aacattttta attgtaactc ctgcattacc ccggttttcg atatccgtac cggcaatcat
      961 gttgtccgga agtgtaattt tagccgcacc ggttgttatc tcaatattta tgccatgctg
     1021 cagattggaa agggctattg ccggaagttc cacattgtag ctttccgtat ccgatttagg
     1081 cagtccaacc cccaatgtct tttttccgtc atcacctgtc agtgtgcctt ccaatgcatt
     1141 atttagagtt tcggtatcaa actctgcttt tacagtacct gtagctgcat ccacagttgc
     1201 aatgatcttc aatccgccta aagttgctga cggaatttcc acagttggaa tttccactgg
     1261 aggaagttcc acagcctggg gttgacgatc cggggtttta tcattatcct tgccgtcatt
     1321 cccattggta cttggcggcg ccgccctgtt cacagtaact gtatatctgt tcgtggatgt
     1381 accgttctga gctataacgt caatctcaat cgtattgctt ccaacattta gcggaatggc
     1441 ttcggataat tcgtgacttc ttagaacctt tccgtttacc accactctgg catttacatc
     1501 ccacgttaca gctgctactc gggtgctttc tacgccataa gctacatttt cggtataatt
     1561 atacttgttg cgtataaagc ctgacacggg ttttcctccg atgtacagag actgtagtgt
     1621 ggcatcgtct ccgggtttat aaccttctac cgctgttacc atcagcctgt cgcttagacc
     1681 tccccagaag gtaactttaa attgcacaaa atcgccatct ctctgcaaaa agcgggagag
     1741 gtaatctttc ttaatgatca ggactttcat acgtttgtta tcatcataag taacggtgta
     1801 gtcgatattc tgtctaagac tatctcctcc acagaccaca tcgatcactt cactaactct
     1861 gtcggcactg atataggttt ttatatcggc aggactatct aatctatatg ctgctgcaat
     1921 cggatccagt aaagtataaa acggctcaaa taccgttaca tacaactggt atttcagatt
     1981 tttcggattt gcttctctaa agggatcaat atttaactcc ccggtaaatt tatatgttac
     2041 cggacgattc ttgttgtata acggtgtacc gtcatcccag gttacaccca cctcttttct
     2101 tgttccatca tgataaactg cagtcacctt ttccggcaag gggatatttt ccctttcagt
     2161 gccatatttc accataatgg cggtaatgtt cggctctatt tctttaataa cttcgatttg
     2221 aattatcgat aactctacga ttacatcaaa ggttttgaca gcctgattgc cgcactcatc
     2281 cgtggctgtt atcgtaacag tggtacgtcc gatgggatat tcagaacctg aaggccggga
     2341 tgcccataca gtaactgaac agccggggtg agagtcagat gtcacgatat aataagtcaa
     2401 gtaccacgtt atctgataaa tttgtagcga aaacaatact gtcacctatt tctaagtcat
     2461 tccttttgtc ataaacacgg attgtaggcg gtgtagtatg cttgacggtg atgtagaaag
     2521 tgccctctgc cctgttcccg gatgaatcct cagcctcaac cttgacttcc gttgtcttta
     2581 taggaaagaa agcattgtta atgtcactaa taggtacccc atcagcagta gcggttaccg
     2641 gaacataacc gtcctcttcg tcgtaggcat aaatcggcaa agagataacg gcaccgtccg
     2701 gtaaattagg ttctaccgtc atgtcttccg gcaggaacag tactggagga tttgtatcgg
     2761 gtggcctgcg aataacggta atggtgtagg ttaggtaagt caaactgtca taagccatca
     2821 cgtaaacaac tatgatgttt tctccttcct ccaacggtac ctcgcaattt cttccttttt
     2881 cacctctgat actgattatt gccccttcat cgttgggtat ggcagagacg gtaaccttat
     2941 ccacttgatt tttcacacta accgtatatt cggtggtgtc gggttggtat aagccttcca
     3001 gcaggttctc cgaaggatcg cagtcggaag cgtaaagtct gagcccgtct ccgtctctta
     3061 gcattgcttc tttggatatt tcatgtttaa tgtaaaactc tattctcctt tgtgggccat
     3121 tctcagtctc aaggtacaag acccatactc tatttacatc cagcacagca tatgcggttg
     3181 agtgatccaa gtaggtaaga tccgcatctt ttaaactgtt cttgtcctta attctcatct
     3241 gcatatcagt cgctgaaaga ggcttggtaa cctcaaaaat tatactatag gtaacatgtc
     3301 cgttaatttt ataaggcacg ttcactttat aactatcgca gtcattgctg aaggcaggtt
     3361 caaaatcgat atcctgcccc tcatagtaaa acctgatact atcaagtctc tggaccagtt
     3421 catcgggtat tttccgttct atcctgagaa cataatcttt ggtactgaga ttgggagcaa
     3481 caacggtgac ttttatatga tttgtgccgt aatccagcgg aacgggataa taatcatatt
     3541 gctgtccgtt taccagtacc cctgccattg ggctgcttgc atctgcttcc aggcaaatcg
     3601 tttttgtttc ctcgggaacc tcgatactgt attccgtgac gtcaggatca aagttttgag
     3661 aaagggcaaa ctcctgtttt ctgtccgatg taaaaacctt taaactctct aaatcgttat
     3721 tgctgtcggt accgtttatc attacaagat acgcacctac attgtttgat tcttcgggct
     3781 ttaccgtaaa gaatattgtg tgttcttctt ccggattcaa catcttcgtc ttgctggtcc
     3841 aaccctcact ttccgtcaag tcatcggtct gtgtctggga cggattccat cctaaaacta
     3901 ctcttgaaga cggattttcg ggttcagcag taaatttcac gggttttaag tcctgtccct
     3961 gtccaagatt aacagaatat accctcacat tggggtcaaa agccggtgac agctccacct
     4021 cctcgttgtc aacccatatc ttcaggtttt taagcctgtc aaactcggat tccgcacggt
     4081 taactatcaa agtgtaaaca acagcctcat tgtacttatt catcaccgta acgggtatct
     4141 cattgcgtcc acatattaat gcctcggttt ttgttcttct tcctctgtat cctgcaccta
     4201 ttgtgtctgt ccccacataa acttcatcat cggggttcac ggcgattgca gtaatctcca
     4261 cagaagtaac ttccggagga acatccatgg tatatatttc gaagtttatg tcaaaggccg
     4321 gaaaaaactg gccgttagtt actcttaggc ttgccagtct cggaggtccc tggctgcggt
     4381 cctcattaac ggtatattca ttattccttt cgtcatctct ctcatcttca ccccataccc
     4441 aagtggtctt ttccacatcg gatttgacca tatccggtac tgaatcaacc aatggaactc
     4501 tccctccgta tacatcgtcg gaagtgctgt caatgatttc atctgtatcc cctaacaggc
     4561 tcgcatcatt tcccatttcg ttcaacccat aattgacaat attcagcgag ttgataccaa
     4621 cagcctgcac ctttggtggg agaacaacca ttgaccacgc cataatcata accagagata
     4681 gtacaactat ttttttcagc aattcaattt ttcgtttccg agaattattt ttttcaatcc
     4741 tgacaccccc ctccgtacag cctttcggta taacaaaacg ttcagcatga aaatacttct
     4801 cagacatata aatcctcaca gaacaccctc cttttctaga aattttcaat ctgtaggtaa
     4861 aaaaaatgca ctcattgctg tgcccattat aaaaaaccgg ctaccaccaa aagtgccacc
     4921 aaaacaaaaa atcttaaatt ttattctttt ttccttcctt tgccgcatca acacgatgcc
     4981 cctgatagcc caaacccgta caacggtgca aaagctccat aatttttatc ctttcaccgg
     5041 attcccgata gctttccatc aaggtttccg ctgcctgaag aaatgtatcc tcacagatca
     5101 gtctggtact ttccgcccac aaggcttcca tgtcatccag ataacgtccg gtgtaatggt
     5161 caaccacctt ttgagcgttt tgaggcgaag gatttttccg aaattcgcag gccaccttcc
     5221 ggaaagcatc atagtcgcat acaatttcct ccatgtccaa cttgtatatg ccttttttat
     5281 aaacaatggg gttttttgct cctaagctct caaaggctcg acgaatttca ccccgtcggg
     5341 tgtggaacat gccggtaaca tctccttcac tgtcatgcca caagtcggaa aagatccgat
     5401 cacggcttac accttcccgg tgctccaaga gataggccag aagttccctg gatttctggg
     5461 tgcggatttt gacgcaggag caccggtcgt gagccggagc aatgtaaaaa gttcccatgg
     5521 tgtttatgta aacccgctgt gttgtgtaac cgccgtatac aagcatctcc cgcacgaatt
     5581 ccgggcttat tccccgcttc aatgcaaatt tcagcacttc cccgaaatga gtcttaagct
     5641 ggaaatagta gcggtggccg tattgctgtg acagtgtaag acacctccgg gtgcattcct
     5701 ctgccctcgc ctcgtctcct tcccggagaa atacagctgc aagctcacca tagcaaacca
     5761 taggtatatt cagggtaccg tacctttcgc cgatttcgat ggacttctt
//
LOCUS       DAOOJC010000108         5770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_11266_length_5770_cov_3.897638, whole genome shotgun sequence.
ACCESSION   DAOOJC010000108 DAOOJC010000000
VERSION     DAOOJC010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11266_length_5770_cov_3.897638"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..904
                     /gene="mreC"
                     /locus_tag="PLA01_04290"
     CDS             74..904
                     /gene="mreC"
                     /locus_tag="PLA01_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014522629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPD00552.1"
                     /translation="MLTVTVIVLIIMGLSSRKNSKLRYVSNAFTVVLTPFQELINYIG
                     NQINEASAYLKDIKAVNEENEALKVRIAELEDEVKELSGYKIKINELKEALNIKQQFS
                     DVEFLGANIIAKDAGNWFNIFTIDRGISDKISVDSTVITSNGLVGRVVSTDLISARVV
                     SIIDEDSTVSARIAKTGNLVFVRGDLKLKEQGLCRLENIFPDVDISVGDTIETSGLGG
                     IYPKGITIGTVKEIRRKTSDLNRYAIIEPAVDFKRLEEVFVLIGKDNIEKAGETEDEG
                     "
     gene            894..1409
                     /gene="mreD"
                     /locus_tag="PLA01_04295"
     CDS             894..1409
                     /gene="mreD"
                     /locus_tag="PLA01_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPD00553.1"
                     /translation="MRVKIVSFTVLIFFITLVQSTVLDNIRVFNVKPNLLMVFIVSAA
                     LLGSNVEGAVVGFFCGLAQDMISGGPIGFYALLGLYLGFGIGSINERLYRENVLVTIF
                     VTFLSTVVYEFTVYFLSSLTKGSIELVFPFTHVILPEALYNSVVSIVIFIVVLKIKRW
                     SEKVTKLSRRY"
     gene            1445..3580
                     /gene="mrdA"
                     /locus_tag="PLA01_04300"
     CDS             1445..3580
                     /gene="mrdA"
                     /locus_tag="PLA01_04300"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPD00554.1"
                     /translation="MGEKENKNNKINERFKVVVVGLTLAFVLILLQLINLQIIRGEYY
                     DEQSQMKLLSERDIVAPRGNIVDRNGVPIAINRIGYTVKIAKTGLNENEKSELILKLI
                     DVFEKNGDSYEQNLSNYLTFNPLAFGTKLKSQEALENWKKDMVMKKEDMELLGTPVDV
                     FKYFRKKFYIEDKYNDEEAYKIMIIKYDMLIKGYTATNPLLIAKDVSAQTVAQIEERH
                     HEFPGVIIDVVPQRKYVNASPVGHILGYIGVISGEEYQQYKDNGYGMNDMIGKNGIEK
                     TQEVQLRGINGKKRVEVDTNGRLTAELSSEPAIPGNDVVLTIDLRLQKAAMESLKQNI
                     ELIRSQADNKKNFGDACAGAAVAIDVNSGEVLALASYPTYDPSVFLAGSQDKEAQKTI
                     QDLIQGKGEYSWYNRAIQGRYAPGSTFKPLTGIAGLETGAITPQNSYITDKGTHIIGG
                     WTFKCMEYPTYGHGRIDLVKALATSCNIYFHELGVKTRIENIDLWAKRFGLGEYTGIE
                     LPSENKGIRANMTTKRELRNDDWRPADTAQTAIGQFDNAFTPIQLASYVSTLANGGKK
                     YKAHIVKEIRKHDGSIVLKSEPEYEDLQIKKETIELVHRGMVEVANAQDGTANKIFDD
                     LKPIIVAGKTGTPETGFEHLGQSSNGLFIAYAPADNPQIAVAVVIEHGVWGSYAAPVA
                     KDILKEYFGMNSDNLPQDGIVPDQAQLIR"
     gene            3693..4376
                     /gene="minC"
                     /locus_tag="PLA01_04305"
     CDS             3693..4376
                     /gene="minC"
                     /locus_tag="PLA01_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HPD00555.1"
                     /translation="MKDSSVIFKGSLDCLTIIMKEEEDFSTILESIEKKIESSGRFFK
                     GTTLSVKYRGKKLSTEEENKIFELLKNKSGANIKDFEEDTEEPIRVEKEVKPPLPHSK
                     IRMSTFYFKNLEEGITKFHRGTVRSGQSMKFDGNLVVIGDVNPGGEVAATGNVVVMGS
                     LRGVVHAGSNGNREALVVALNLQPTQLRIADVITRSPDEKETMGQFVPELAYIKGGTV
                     LIERYLPAR"
     gene            4702..5502
                     /gene="minD"
                     /locus_tag="PLA01_04310"
     CDS             4702..5502
                     /gene="minD"
                     /locus_tag="PLA01_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HPD00556.1"
                     /translation="MGEVIVITSGKGGVGKTTTTANIGTGLALQGKKVVLIDTDIGLR
                     NLDVVMGLENRIVYDIVDVIEGNCRLKQALIKDKRYEGLHLLPAAQTRDKSAIKPEEM
                     INLCGELKKEFDYILVDCPAGIEQGFKNAIAAADRAIVVTTPEVSAVRDADRIIGLLE
                     ANDLRDQKLLINRVRPDMVKRGDMMSIDDIIDILAIDLIGVVPDDEKIIISTNRGEPA
                     VTDNKSQAGEAYRGIARRIMGENVPILNLQSEDGFMFKIKKLFGLKVN"
     gene            5516..>5770
                     /gene="minE"
                     /locus_tag="PLA01_04315"
     CDS             5516..>5770
                     /gene="minE"
                     /locus_tag="PLA01_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HPD00557.1"
                     /translation="MLLDLSKLFGRSKNSKDLAKERLKLVLIHDRANVSPQFLEMVKG
                     EIIKVISNYMDVDEDSLDIQMTRTRSEEGDRVVPALVANIP"
BASE COUNT         1865 a          861 c         1432 g         1612 t
ORIGIN      
        1 atttctccta agaaactcag atagaaggga gataatcttg ttgcggcgga ttataaaaag
       61 caaaattttt attttgctga cggttacagt tattgttttg attataatgg ggttgtcttc
      121 gaggaaaaac agcaagctaa gatatgttag caatgccttt actgttgtgc ttactccctt
      181 tcaggaactc attaattaca ttggcaatca aataaatgag gcttcggcct atttaaagga
      241 tatcaaggcg gtaaatgagg aaaatgaggc cttgaaggta agaattgccg aattggaaga
      301 tgaagtaaag gagctttcag gttataaaat taaaataaat gagttgaaag aggcattgaa
      361 tataaagcag caattcagtg atgttgaatt tctgggagca aacattattg caaaggatgc
      421 cggcaactgg ttcaatatct ttacaataga caggggaata agcgacaaga ttagtgttga
      481 ctcaacagtt ataacgagta atggacttgt agggagggta gtaagcactg accttatttc
      541 tgcgagggtg gtatcgatta ttgatgaaga cagtaccgta agtgcaagaa ttgccaaaac
      601 cggcaatctg gtattcgtaa gaggcgattt gaagctaaaa gagcagggat tgtgcaggct
      661 ggaaaacata tttcccgatg tggatatatc cgttggcgat acaatagaga cttcgggact
      721 gggaggtata tatccaaagg gtattaccat aggaactgtc aaggaaatca ggcgtaaaac
      781 cagtgatttg aataggtatg caattatcga accggccgtg gattttaagc ggcttgagga
      841 agtatttgtt cttattggca aggataatat agaaaaagca ggcgaaacgg aagatgaggg
      901 ttaaaattgt atcttttacg gttttgatat tctttataac gctagttcag tcaacggttc
      961 tggataatat cagagtgttt aatgttaagc ccaatttgtt aatggttttt attgtttcgg
     1021 ccgcacttct cggcagcaac gtggaaggtg ctgtggtagg ttttttttgt ggcctggctc
     1081 aggatatgat ttcaggaggt cccataggat tttatgctct tttagggctc tatctcgggt
     1141 ttggaatagg ttctataaat gagaggctat acagggaaaa tgtgctggtc acaattttcg
     1201 ttaccttttt atctacggtg gtttatgagt ttacggtcta ttttttaagc agtttgacaa
     1261 aaggctctat agagttggtt tttcctttca cacatgtgat tttacctgaa gcactttaca
     1321 atagtgttgt ttcaattgtt atatttattg tggtattaaa aataaaaaga tggtctgaaa
     1381 aagtaacgaa gctatccaga cgttattaag gagcttattg cagctgttgg caggaggtgg
     1441 aaatgtgggg gagaaggaaa ataaaaataa taaaataaat gaaagattca aggtcgtagt
     1501 agttggtttg accctggctt ttgttttgat tcttcttcag ctaataaacc ttcagattat
     1561 tcgtggcgaa tactatgatg aacaatcgca gatgaagctg ctttcggaga gggatatagt
     1621 tgctcccagg ggaaatatag tagacagaaa cggcgtaccg attgcaataa acaggattgg
     1681 gtatacggtg aaaatagcaa aaaccggttt gaatgaaaac gaaaaaagtg agttgatatt
     1741 aaagcttatt gatgtttttg agaaaaacgg ggactcttac gaacaaaatc taagtaatta
     1801 tctgacattt aatcctttgg cattcggaac taaactcaaa tcgcaagagg ctttggaaaa
     1861 ctggaaaaaa gacatggtca tgaaaaagga agatatggag ttgcttggaa caccggttga
     1921 tgtctttaaa tattttagaa agaagttcta tatagaagat aagtataacg atgaagaggc
     1981 ctacaaaata atgattatca aatacgatat gcttatcaag ggatatactg caaccaatcc
     2041 tttacttatt gcaaaggatg tcagtgcaca aaccgttgca caaatcgaag aaaggcatca
     2101 tgaatttccg ggggtaatca ttgatgttgt gccccagagg aagtatgtaa atgcaagccc
     2161 ggtagggcat attctgggtt atattggcgt gataagcggt gaggagtatc agcagtataa
     2221 agacaacgga tatggcatga atgatatgat tggaaagaat ggtatagaaa agacccagga
     2281 agttcagtta agggggataa acgggaaaaa gagggttgag gttgatacaa acggaagact
     2341 tactgctgag cttagcagtg aaccggcaat tcccggaaac gatgtggtgc ttactataga
     2401 ccttcggctt cagaaagcag cgatggagtc gttgaagcag aatattgagt tgataaggtc
     2461 gcaggcagac aacaagaaaa attttggaga tgcctgtgcc ggtgcggcgg ttgctattga
     2521 tgtaaattcc ggagaggttc tggcactggc aagttatccg acctatgacc cgtctgtttt
     2581 tcttgcaggt tcacaggata aggaagcaca aaaaacaata caagacctga ttcaaggcaa
     2641 gggtgagtat tcatggtata acagggcaat acagggaaga tatgcacccg gttctacctt
     2701 caagcccctg acgggaattg caggacttga gacaggagct ataactcctc agaacagtta
     2761 tattaccgat aaaggaaccc atattatagg gggttggact tttaaatgta tggagtaccc
     2821 tacctatggg catgggagaa ttgaccttgt taaagccttg gctacttcgt gcaatatcta
     2881 ttttcatgaa ctgggagtta agaccaggat tgaaaacatt gatttatggg caaagcgttt
     2941 tggacttgga gagtacacgg ggattgaatt gccctcggaa aataagggca ttagggcgaa
     3001 catgactacc aaaagagagt tgagaaacga tgactggaga ccggctgaca cggcccaaac
     3061 tgctatagga cagtttgaca atgcatttac accgattcaa ctggcaagtt atgtaagcac
     3121 cttggcaaac ggagggaaaa agtataaagc ccatattgtc aaggaaatac gtaaacatga
     3181 cggctcgatt gtgcttaaaa gcgagcctga atatgaagat ttacagataa agaaagaaac
     3241 aattgagttg gttcacagag gcatggtgga agttgcaaat gctcaggatg gaactgccaa
     3301 caagattttt gacgatttga aacctattat tgtagcggga aaaaccggta cccctgagac
     3361 gggatttgaa catctgggac agtcttcaaa cggtttgttt attgcctatg ctccggctga
     3421 taatccacaa attgcagtgg cggtggtcat tgagcatggg gtatggggat catatgcagc
     3481 accggttgca aaggacattt taaaggagta ttttggcatg aacagcgaca acttgcctca
     3541 ggacggtata gttcccgatc aggcacagtt aatacgctga cgttgctgcc acagattgac
     3601 tgaaaaagtt ttagttttat ttaaactata taatgtgtaa gttcattcgg ctaaagctga
     3661 attaatcgac atttttattg ggggttataa aaatgaagga tagcagtgtt atttttaaag
     3721 gttctttaga ttgccttacc atcataatga aggaagaaga ggatttcagc actatacttg
     3781 agagtattga aaagaagata gaatcttccg ggaggttttt caagggcacc actctttctg
     3841 ttaagtacag agggaaaaag ctgtcaacag aagaggagaa caagattttt gagcttttga
     3901 agaacaaaag cggtgcaaac atcaaggact tcgaagagga taccgaagaa cctataaggg
     3961 ttgagaaaga agttaaaccg cctttgccgc attcaaagat aaggatgagt actttctatt
     4021 ttaagaacct tgaagaggga atcacaaaat ttcatagagg aacagttcgt tcaggtcaat
     4081 cgatgaagtt tgacggaaac ttggtggtta ttggtgacgt aaatcccgga ggtgaggttg
     4141 ctgcaaccgg aaatgttgtg gttatgggtt cgctgagggg agtggttcat gcaggttcca
     4201 acggaaacag ggaagctttg gtggtagctt tgaatttgca gcctacgcag cttagaatag
     4261 ccgatgttat tacgcggtca ccggacgaaa aagagactat gggccaattt gttccagaat
     4321 tggcttatat taaaggaggc acggttctaa tcgagagata tcttcctgca agataaggtt
     4381 tggtggtgct ggcataatgc aaatttacaa aattgcctgc taatgcttat tagagaaaaa
     4441 tgaagaattg acaaattatt aaaagaaata tatagtttaa ataagagttt tttttatgag
     4501 ttttttaata aatgagtctt cttatttgtt ggtatgtttg tttttgggaa atatgataaa
     4561 attctatgca agcttagtat aattcatgta ttttatcttg atagaaatag ttgattacat
     4621 ctgattttaa tacatacaat caagaacgag gattagataa gagtgtacgt tcttaattga
     4681 atataggtgg gaggtatatt aatgggtgag gttattgtaa taacttctgg caaaggtgga
     4741 gtaggcaaga caacaaccac agcaaatatt ggaacaggtt tggcacttca gggaaaaaaa
     4801 gtggttttaa ttgatactga tataggtctt agaaatttag atgttgtaat gggtctggag
     4861 aacaggattg tgtatgatat tgttgatgtt atcgagggta actgcagatt gaaacaggcc
     4921 ttgataaagg acaagcgtta tgagggactt catctccttc ctgctgccca gaccagggat
     4981 aagtccgcca ttaagcctga ggaaatgatt aacttatgtg gtgaattaaa aaaggagttt
     5041 gattatatac tagttgactg tcctgcgggt atagaacagg gctttaaaaa tgcaatagca
     5101 gcagcggaca gggcaatagt tgttactaca cccgaggttt cggcagtaag ggatgcagac
     5161 aggattatcg gccttttaga ggcaaacgac ttaagagacc aaaagctcct tataaacaga
     5221 gtcagaccgg acatggtaaa acgcggtgac atgatgtcca tcgatgatat tattgatatt
     5281 cttgctattg accttattgg agttgttccg gatgacgaaa aaataataat ttccacaaac
     5341 aggggagagc ctgccgtaac cgacaataag tctcaggcag gagaggcata tagaggaata
     5401 gcaagaagga ttatgggtga aaatgttccc attcttaacc tgcagtcaga agacggattt
     5461 atgtttaaaa taaaaaagct ttttggatta aaagtaaatt aataggagga gacttatgtt
     5521 gctagattta tcgaaattgt ttggtagatc gaaaaactcg aaagatcttg ccaaggaaag
     5581 acttaaacta gtgctaatcc acgacagagc aaacgtttct cctcagtttt tagagatggt
     5641 caaaggggaa atcatcaagg ttatttccaa ctatatggat gtggatgagg attctcttga
     5701 cattcaaatg accagaacta ggagtgaaga aggggatagg gttgtaccgg cattggttgc
     5761 aaacatccct
//
LOCUS       DAOOJC010000109         5749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_11313_length_5749_cov_3.691605, whole genome shotgun sequence.
ACCESSION   DAOOJC010000109 DAOOJC010000000
VERSION     DAOOJC010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11313_length_5749_cov_3.691605"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PLA01_04320"
     CDS             complement(<1..590)
                     /locus_tag="PLA01_04320"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPD00558.1"
                     /translation="MKDKILRKLKDAAGNYVSGEQLGIDLDVSRTAVWKHIKELKSEG
                     YVIESSSRKGYRLSSVPDIVNSSEVSDGLNTRIIGKNIHCLAQVDSTNNYAKRLAQEG
                     CEEGTVVIADCQTEGRGRMGRSWHSMGNKGIWMSMVLRPVVGPEEVQIITLAASIAVV
                     SAINDVLDIKAGIKWPNDVILDGKKVCGILTEMSME"
     gene            complement(703..1317)
                     /locus_tag="PLA01_04325"
     CDS             complement(703..1317)
                     /locus_tag="PLA01_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00559.1"
                     /translation="MVEVYYYIPAEEVENAIECGLKLSVWFDKEVRIGIDNKKCLSAL
                     LNPKDDMEKYQSANLRCVKLELYPRYCFVADRYLYEIGLNHPEVMKLYLDSVMPIGNY
                     TFGLYRLPECLVTSTVIGDQISLLDKRLDSPVLVDKSEELYINNIIEDYREENPDFND
                     TLLYYYYCNLVQKGKLVKVEEEGKDIVVFLDNEIGRAYTIKKPV"
     gene            complement(1438..1950)
                     /gene="folK"
                     /locus_tag="PLA01_04330"
     CDS             complement(1438..1950)
                     /gene="folK"
                     /locus_tag="PLA01_04330"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPD00560.1"
                     /translation="MNHKVYLSLGSNMGNRENYLISALESISKTKNVCLDNISSIYET
                     DPVGYTDQNRFLNMAVSIGTSLDPEALLTESQRIERLLERKREIRWGPRTIDIDMLLY
                     DDLTFETPKLTIPHPRMFERAFVLVPLKEICSDMELFGIIIDDAIKKCSDRDGIRLYK
                     KITICDFRRI"
     gene            complement(1940..2308)
                     /gene="folB"
                     /locus_tag="PLA01_04335"
     CDS             complement(1940..2308)
                     /gene="folB"
                     /locus_tag="PLA01_04335"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HPD00561.1"
                     /translation="MDKIVFNNIRLFGYHGVFHSEREKGQNFYIDVEAFLDLKEAGET
                     DDLEKTVDYSKIYEIIKNINENNKFRLIERFAHIISREILSTFKEIKRIIVRVRKPDA
                     PIDGDFSWVGVEIERDRNES"
     gene            complement(2332..3513)
                     /gene="folP"
                     /locus_tag="PLA01_04340"
     CDS             complement(2332..3513)
                     /gene="folP"
                     /locus_tag="PLA01_04340"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPD00562.1"
                     /translation="MVNARIIYIDNMHEAREEIRRIGVDASSIPWLSPKALFITMKLE
                     NISTYAANIIKQEMLGKGGDAAVNRGVVNFNVESSDVLLMGTYSQYKRLVYKLGMQNT
                     SLKKIADEIQRLLEGLEEGKPKYFECGKYRLPMGEKTYVMGILNVTPDSFSDGGQFIN
                     PESAVKRAKEMVEHGADIIDIGGESTRPGHQPVDVFEEINRVVPVIEILSKELNVPVS
                     IDTSKALVAEKAVAAGACIVNDVWGLQRDSSMSETISKNGAGVIMMHNSDSKEYYDIM
                     GDIIRFLRKSIEIAEEAGITRENMVVDPGIGFGKNLEHNLEVMRRLKELGTLNLPVLL
                     GTSRKSMIGNVLELPVNERLEGTAATITLGIANGVDIVRVHDVKEMTRVTRMTDAMVR
                     A"
     gene            complement(3580..4068)
                     /locus_tag="PLA01_04345"
     CDS             complement(3580..4068)
                     /locus_tag="PLA01_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPD00563.1"
                     /translation="MKIGVVSDTHIPSRARALPKALMEGLLGVDLIIHAGDLLKDYVL
                     YELEEIAPVEAVAGNNDDEYIRSMLPHKKIIKAGSFRIGITHGYGRGSNTLYAALATF
                     MRDNVDCVVFGHSHVPFNDRVNGILCVNPGSPTDKRSQEHYSYGIIKVEDSDISAEIY
                     YF"
     gene            complement(4074..5186)
                     /locus_tag="PLA01_04350"
     CDS             complement(4074..5186)
                     /locus_tag="PLA01_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HPD00564.1"
                     /translation="MNFKFKIGTKAFFGKGCIESNKAVFKEFGKKAFIVTGNNSAKAS
                     GALEDVVEALKECGVEYRIYDRVLNNPTLENVKEGGELAREFGADFIIGIGGGSPLDA
                     SKAVSVLACNSIEPVDLYKNIFENKPLPIIAIPTTAGTGSEITPYSILTRNDMKTKKS
                     FGNEDTFPAVAFLDARYTESMSRQTTVDTAMDAFTHALEGYLGKKSTPVSDILAVEAI
                     GIFGECIDSLLNNRLDFEVRERLLYMSMLGGMVISHTGTTIIHGLGYSLTYFKDIPHG
                     RANGYLVREYLKYNYEAAGEKINNVLKLLKVSSVDELGDKIGGLLPCDITLSDEEIEM
                     YAGLAMQQRSTSSNARTVVLEDLGEILRKVFKGEGN"
     gene            5394..>5749
                     /locus_tag="PLA01_04355"
     CDS             5394..>5749
                     /locus_tag="PLA01_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HPD00565.1"
                     /translation="MGKEIERKFIVEGDTYKELSQGTLYRQGYLCSDSIRTVRVRLCG
                     NKGYITVKGKTQGAARPEYEYEIPVDDANEMLENLCEKPLIEKIRYKYSFEGFIWEID
                     EFLGENDGLVIAEIELP"
BASE COUNT         1583 a         1459 c          928 g         1779 t
ORIGIN      
        1 atttccatac tcatttcagt cagtatgccg caaacctttt tcccatccaa tattacatca
       61 ttgggccatt taattccagc tttgatgtcc agcacgtcgt ttatagccga aacaaccgct
      121 attgaagcgg caagagtaat gatttgaact tcttccggac cgacaacagg acgcaaaacc
      181 atggacatcc atattccctt attacccatg gaatgccagc tcctgcccat tctgcccctt
      241 ccttcggtct gacaatcggc aataactacg gtaccttctt cgcagccttc ctgtgcaagc
      301 cttttggcat aattgtttgt cgaatccacc tgtgccaagc aatgaatatt tttgccgata
      361 attcttgtat taagaccgtc gctcacttcc gacgaattca ctatatccgg caccgagctt
      421 aacctatagc cttttctgga agaagattcg ataacataac cttcactttt taactcctta
      481 atgtgcttcc ataccgcagt tctggataca tccaagtcta tgcccagctg ctcaccggaa
      541 acataatttc ctgctgcatc cttcaatttt cgcaatattt tgtctttcac ctttatcctc
      601 tcaaccaaac caaatattgg tacttttagt aaaaatcatt tcataaaatt aaatttcacc
      661 ggtattacat gagtagtctt aaaaagacca agcctgctgc ttttaaacag gtttctttat
      721 tgtataggcc ctgcctatct cattgtcaag gaaaacaact atgtcctttc cttcctcctc
      781 aacctttaca agcttccctt tttgtaccaa attgcagtaa taataataca gcagtgtgtc
      841 attaaaatcg ggattttctt ctctgtagtc ctcaataatg ttgtttatat atagctcctc
      901 tgatttatcg accaagactg gggaatccag ccttttatcc agaaggctta tctggtcacc
      961 tataacagta ctggtaacaa ggcattccgg aagtctgtat aaaccgaagg tataattccc
     1021 gatcggcatc accgaatcaa ggtaaagctt cataacctca gggtgattga gccctatttc
     1081 gtaaagatac ctatccgcta caaaacaata cctggggtac aattctagct ttacacacct
     1141 caagttagca gactgatact tttccatatc atccttcgga tttaaaagtg ccgatagaca
     1201 ctttttattg tcaatcccta tccgtacttc cttatcaaac cacacggaca gcttcagacc
     1261 acattctata gcattttcaa cttcctcagc aggaatatag tagtaaacct ctaccataag
     1321 gtcacccccc catcggaacc ttcaattagt ctttcataaa atgccgtcaa ccttcagtat
     1381 ataataacaa caggaaacta caaccatcct tattttattg tatcattaat aattgtatca
     1441 gattctgcgg aaatcacaaa ttgtaatttt tttatacaac cttattccat ccctgtcact
     1501 gcactttttt attgcatcat caataattat accaaacaac tccatgtcac tacaaatttc
     1561 tttcaacggt acaagcacaa acgcccgctc aaacattcgg ggatggggaa tagtcagctt
     1621 tggagtttca aaggtcaaat catcataaag aagcatgtca atatcgattg ttcttggtcc
     1681 ccatcgtatt tcccgttttc tttccagaag cctctcaatc ctttgggact ccgtaagaag
     1741 tgcttccgga tccaaagaag taccgattga tacagccata tttaaaaagc ggttctgatc
     1801 ggtatagccc acaggatcag tttcataaat gcttgaaata ttgtcaaggc acacattttt
     1861 agtcttagaa atactttcaa gggcactaat caaataattc tctctgtttc ccatgtttga
     1921 gcccagagac aaataaacct tatgattcat ttctgtccct ctcaatctca acccctaccc
     1981 atgagaaatc tccgtcaata ggagcatccg gctttctgac gcggacaata attcgtttta
     2041 tttccttaaa ggtcgacagt atttcccggg aaattatatg ggcaaacctc tcaataagtc
     2101 gaaatttgtt gttttcattt atattcttaa ttatttcata tatcttcgaa taatccactg
     2161 tcttttccaa atcatcggtt tctccggctt ctttaagatc caaaaaagct tcaacatcaa
     2221 tatagaaatt ctgtcccttt tccctctccg aatgaaatac tccatggtaa ccgaataatc
     2281 ttatattatt aaatactatt ttatccacaa tactcctccc atgataaaat cctatgctct
     2341 caccatcgca tcggtcattc ttgtgacccg ggtcatctcc ttaacatcat gaaccctgac
     2401 aatatccact ccgttggcaa ttcctagagt aatggtcgct gcagtaccct caagcctttc
     2461 attgaccggc agttccagaa cattgcctat catggatttt cttgatgtcc caagaagtac
     2521 cggaagatta agggtaccta gctccttaag ccttctcatt acctccaggt tgtgctctaa
     2581 atttttaccg aatcctatac ccggatccac caccatgttt tctcttgtga taccggcctc
     2641 ttctgctatt tcaatgctct ttctcaagaa ccttatgatg tctcccatga tatcataata
     2701 ttctttgctg tcgctgttat gcatcattat tactccggca ccgtttttgg atattgtctc
     2761 cgacatggaa gaatcccttt gaagccccca aacatcatta acaatacatg caccggcagc
     2821 aacagccttt tctgccacca atgcctttga agtatctata gatacgggaa cattcaactc
     2881 ctttgagagt atttcaatta caggcaccac ccggtttatt tcctcaaaca catccaccgg
     2941 ctgatgtccg ggccttgttg actccccacc gatatctata atatcggcac cgtgctccac
     3001 catctcttta gcccgcttta cagcactctc cggattaatg aattgccctc cgtcagaaaa
     3061 ggaatccggt gtcacattta aaatccccat cacataggtt ttctccccca tcggaagtct
     3121 gtattttcca cactcgaagt atttcggttt tccttcttcc agcccttcta atagcctctg
     3181 tatttcatcg gcgatttttt taaggcttgt gttctgcatt ccaagcttat aaaccagtct
     3241 tttgtactgg ctgtatgtac ccatcaagag aacatcggaa ctttcgacat taaagttcac
     3301 cacgcctctg tttaccgcag catcgccgcc cttgcccagc atctcctgtt ttattatgtt
     3361 tgccgcatag gtgcttatgt tctcaagctt catggttata aacaacgcct tcggtgaaag
     3421 ccaaggtatg cttgatgcgt ccacacctat tctgcggatt tcctcgcggg cctcatgcat
     3481 attgtcaata taaataattc ttgcattaac catggggcta ctcacttcct cccgttagtt
     3541 atttttattt caagctaact tcgataaaac tgtttatagc taaaagtaat atatttctgc
     3601 actaatgtcc gaatcctcca cttttataat cccataggaa taatgctcct gtgacctttt
     3661 atcggtggga gaaccggggt tcacacataa tatgccgtta accctatcat taaaaggtac
     3721 atggctgtgt ccaaaaacca cacaatccac attatctctc ataaacgttg ccaatgccgc
     3781 atataaagta ttgcttcccc tcccataccc gtgagtaatc cctatcctga agctgccggc
     3841 ctttataatc ttcttatggg gcagcatgct ccgtatatat tcatcatcat tatttcccgc
     3901 caccgcttcg accggtgcta tctcctcaag ctcgtacagc acataatcct ttaaaaggtc
     3961 cccggcatga ataataaggt caacccccaa aagtccttcc ataagggcct tgggtagagc
     4021 ccttgctctg gaaggtatat gcgtatccga tacaactcct attttcaata agctcaattt
     4081 ccttcacctt taaaaacctt tctcaatatt tccccaagat cctccaaaac cactgtcctg
     4141 gcattggaag aagtacttct ctgctgcatt gcaaggcctg catacatctc aatttcttca
     4201 tcgctcaaag ttatgtcgca cggaagaagt ccgcctattt tatcacccag ctcgtcaacg
     4261 gatgacacct ttaaaagctt cagtacattg ttaatctttt ctcctgcagc ctcgtaatta
     4321 tactttaagt actccctgac aagataaccg ttggcccttc catggggaat atccttaaaa
     4381 tacgtaaggg aataacccaa tccgtgtatt attgtggtac cggtatggga aatgaccatt
     4441 cccccaagca tcgacatgta caaaagcctt tccctcactt caaaatcgag ccggttgttt
     4501 aaaaggctgt caatacattc cccgaatatt ccgatggcct caactgccag tatatcactg
     4561 accggagtgc ttttctttcc gagatagccc tcaagggcgt gggtaaatgc atccatggcc
     4621 gtatcaaccg ttgtttgccg ggacatgctt tcggtatacc gtgcatcaag gaatgccact
     4681 gccggaaatg tatcctcatt tccaaagctc ttttttgttt tcatgtcatt cctcgtaaga
     4741 atggaatatg gggttatttc gcttccggtt ccagctgttg tgggaattgc tataatcggc
     4801 agcggcttgt tttcaaatat atttttatac aaatccacag gctcaatact gttgcatgcc
     4861 aaaaccgata ccgcctttga cgcatccagc ggggagcctc cccctattcc aatgatgaaa
     4921 tcggcaccaa actcccttgc cagttcccct ccttccttta cgttttcaag agtagggttg
     4981 ttcaaaaccc tgtcataaat cctatattcg acaccacact ccttcagtgc ctcaacaaca
     5041 tcctcaaggg ctccgcttgc ttttgcggaa ttatttcccg taacaataaa agccttcttt
     5101 ccaaactcct taaacactgc cttattgctt tctatacacc cttttccgaa aaaagcctta
     5161 gtacctattt taaatttgaa gttcatatgc actcaattcc ccttcttatt tgtcattaac
     5221 aattattact tattataata taataaattc tatttttaaa ggcattgctc gaccccacgg
     5281 agtatttaca ggcggctgga cttgcgtaac ttaagtttct ggaatatttt tctccacatt
     5341 agattgatat gcgtggtata atatatgtac cccaaatata ggaggaatgc tctatgggaa
     5401 aagaaattga aagaaaattt attgttgaag gtgataccta taaagaacta tcccaaggaa
     5461 ctttataccg gcaaggctat ttatgctccg actctatacg cacagtcagg gtccggttat
     5521 gcggtaacaa gggatatata accgttaaag gaaaaactca gggtgcggca agaccggaat
     5581 atgagtatga aattcccgta gacgatgcaa atgaaatgct cgaaaaccta tgcgaaaaac
     5641 ccctcataga aaaaatcaga tataaatatt ctttcgaagg cttcatatgg gaaattgacg
     5701 aatttctcgg tgaaaacgat ggactggtta ttgccgaaat cgagcttcc
//
LOCUS       DAOOJC010000110         5744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_11320_length_5744_cov_3.808402, whole genome shotgun sequence.
ACCESSION   DAOOJC010000110 DAOOJC010000000
VERSION     DAOOJC010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11320_length_5744_cov_3.808402"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..901
                     /locus_tag="PLA01_04360"
     CDS             188..901
                     /locus_tag="PLA01_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD00566.1"
                     /translation="MLIIKRLLGQIRRAIQDYDMIQDGDRVAVGVSGGKDSLTLLVAL
                     RQIKNFLPRKFELEAITLTMGIGEFDTTPIKELCERIDVNYTVEETLIGKIVFEERKE
                     KNPCSLCANLRRGALHNAAKMLNCNKVALAHHRDDVIETLLLSTFYEGRLHTFSPVTY
                     LDRKDLYVIRPLIYTEEKLVKEFIKGNNITTVPNPCQMDGMSRRQYIKDLLANLRKEN
                     REIKNNIFGAIKRSGIDRW"
     gene            1085..3883
                     /locus_tag="PLA01_04365"
     CDS             1085..3883
                     /locus_tag="PLA01_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HPD00567.1"
                     /translation="MKYIRKSVSVLIIISLVIMLSLPGVNAGIIYESKTTEVVTSGVT
                     LETITRFADEGWQKINVLRVNLNNPNVKVDTLTNSESIRNLTNVKALAESGGAVAAIN
                     ASFFSWLSESGKGDPIGPIIQSGKVVYIDEEFNRYNNSMATLAIDNDKNVHYDYWKTS
                     ITLNSPYGGSANVSQYNKLSPSDYNDLTIWTPKWSKYSIGATEQYKDMVEMVVVGNTV
                     MEVRKNLPAVEIPQNGYVIMGRGDNGKFLYNKFQAGDEVNLTISTTIDWETIEMAATG
                     GAMLVKDGKIPSTFSHDASGASRRPRTAAGSSKSGKELILVTVDGDQSAVKGMTMKEL
                     ANLMLDLGAYNAINFDGGGSTTMVSRTPASNNLKVVSNVGGSLRSIATAIGIFSVCPP
                     SSLEGLIIDKEQSNVFINTPVKLSVRGYDTYFNPFAVDQSKIKWSVSGIEGSFEDSTF
                     HPKTSGFGTITASIDGVTSSTGIMVLDKPNRLILSDKELYLTVGSSRTLSVKATDSNG
                     YTSNVNPENITWTLEGDVGKLENNTFTATKTGVGYIKASWGDAQAYCAVSVVEETLHS
                     LDKFEKWNGLFSSAPANLPGSYELSNEQKKSGKYSGKLTYDFSYLEGTRAAYVVFDND
                     GINLGENTTELNMWVYNPRENSNWLRAEVVDAAGKKHLISFCNGMDWTGWRQVSASLR
                     DIDSPTRLTRVYLAQVNPVPEAGYIYIDDLGFKQHKDFPEIDKDTVPEDVFAPDAANK
                     VTELADDSIRFSVIANTIEKPQNMLQKLMNLRFAEGVNADDTIKDPQIISNIKSYDCF
                     DAGKSRFIVLDTSDKSLRTSSSGQWQWFSEKLESFSGDNLFIFMKNPPSTFSDSLEAN
                     LFKKLLVEKKEKTGKNIWVFYNGSTNTYTPENGIRYFSLAQVSTTGLTPENAIDAKYI
                     EVTATGTDVSYQYKSMFE"
     gene            complement(4103..4975)
                     /gene="galU"
                     /locus_tag="PLA01_04370"
     CDS             complement(4103..4975)
                     /gene="galU"
                     /locus_tag="PLA01_04370"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HPD00568.1"
                     /translation="MKVRKAIIPAAGLGTRFLPATKAQPKEMLPIVDKPTIQYIVEEA
                     IMSGIEDIIIISGRSKRAIEDHFDKSYELEQELKNKGKDQLLKEVQDISNLANIHYIR
                     QKEAKGLGHAIYCAKSFIGNEPFAVLLGDDIVDSEVPCIKQLIDVYNEYKTTVLGVQP
                     VSEEDVSKYGIVGGKPVDDRVYKVKDVVEKPDRENAPSNVAILGRYIITPQIFEHLEN
                     VKPGKGGEIQLTDALKSLIQHEAMYAYDFVGKRYDVGNKLGFLQATVEFALKREDLRD
                     DFEAYLKKLLGCEQ"
     gene            5139..>5744
                     /locus_tag="PLA01_04375"
     CDS             5139..>5744
                     /locus_tag="PLA01_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPD00569.1"
                     /translation="MFAGFIKKNTGVLKIIHEVYIRSKDDEVPALAAQLTYYFILSLF
                     PFLIFIITLVSYTPIASERALGSISEMLPPPVYNLVLNVIREVTLSPRKTFLSFGMIT
                     ALWASSNGMNAVIRGLNKAYDTKETRPYWKIRGLSVVSTAALALAIIISLGLLVFGET
                     MGEQLFEFTVFKTAWTALRLCFSFLFMIMVFSLLYMLVPNRR"
BASE COUNT         1713 a         1275 c         1203 g         1553 t
ORIGIN      
        1 attaacaggc tctacaactt gcttcacaat gtttcttcca catttttctt cacataaata
       61 atgtgcaccc gcacttcacc attatttgta acacaatatt aacttgatat ggcaaaacag
      121 ataacttata attatcgtga agagttgtgt gtgattatgt aaacccgttt ggaactgatg
      181 taaagaggtg ttaattatta agagactact gggacaaatc agaagagcaa tacaggacta
      241 cgatatgata caggacggtg acagggtggc tgttggggtt tccgggggaa aggacagtct
      301 gacacttctt gtggccctaa gacagataaa aaatttcctt ccgagaaaat tcgaactgga
      361 agcaataacc ctgaccatgg gaattgggga gtttgatacc actccgataa aagagctttg
      421 cgaaagaatt gacgtaaatt acaccgttga agaaaccctt atcggaaaaa tagtgtttga
      481 agaacgcaag gagaaaaatc cctgctccct ttgtgccaat ctgagacggg gtgccctgca
      541 caatgccgca aagatgctta actgcaacaa ggttgccctt gcccatcaca gggatgatgt
      601 tattgaaacc ctgctgttga gcacctttta tgagggaagg ctacacacct tttcaccggt
      661 gacttatctt gacagaaagg acctttacgt gataaggccc ctgatttata ctgaagaaaa
      721 acttgttaaa gaatttataa aagggaataa tataactaca gtgccaaatc cttgtcaaat
      781 ggacggcatg tccaggcggc agtatataaa ggatcttttg gcaaacttaa gaaaggaaaa
      841 ccgagaaata aaaaacaaca ttttcggtgc aattaaacgt tccggtatag acagatggta
      901 gccgggcagc atgtgtctga aagaccgttg ctggcttccg attgttgcag cacacaaaaa
      961 agggtaacct cggcaacagt tgtccaaaag accgttaaaa cccggacggt ttttggcagt
     1021 aaacttagct tataacatgc agcctgttcg acaggccgta aaaatattac aggaggatgt
     1081 cgttatgaag tacataagaa aaagcgtgtc ggttctgata ataatctccc ttgttataat
     1141 gttatcatta cccggcgtaa atgcaggaat tatctatgaa tctaaaacca cggaggttgt
     1201 aacctcaggt gtaactttag aaaccattac aaggtttgcc gacgagggat ggcaaaaaat
     1261 caatgtgtta agggtaaatc tgaacaaccc aaacgtcaag gttgacaccc tgaccaattc
     1321 agagtccata agaaatctga ccaatgtgaa agccctggct gaatccggcg gtgctgtggc
     1381 agccataaac gccagcttct tcagctggtt gagtgaatcc ggaaagggag atcccatcgg
     1441 tcctatcata caatccggaa aagtggtgta catcgatgag gagttcaaca gatacaacaa
     1501 ttccatggca actttggcaa tagacaacga taaaaatgta cattatgatt actggaaaac
     1561 ctcaatcaca ttaaattctc cctacggagg ctcggctaat gtttcacaat acaacaagct
     1621 tagtccgtca gactataacg acctgaccat atggacccct aaatggagta aatattctat
     1681 aggtgcgacg gagcaatata aagatatggt agaaatggtt gttgtcggca atactgtcat
     1741 ggaagtccgc aaaaaccttc ccgctgttga aatcccgcaa aacggttatg tgataatggg
     1801 tagaggtgat aacggtaaat tcctttacaa caaattccag gcaggagacg aagtaaatct
     1861 caccatctca acaaccattg actgggaaac tatagaaatg gcagctacag gcggtgccat
     1921 gctagtaaag gacggcaaaa ttccgtcaac tttttcccat gatgcctcgg gagcaagccg
     1981 ccgtcccaga actgccgccg gaagctccaa atccggtaaa gagctgatac tggttaccgt
     2041 tgacggtgac caatccgcag tcaaaggtat gaccatgaaa gaactggcaa acctgatgct
     2101 ggatctcggt gcctacaatg caataaactt tgatggcggc ggctcgacaa caatggtgtc
     2161 ccggacaccg gcaagcaata accttaaagt tgtaagcaat gtgggaggtt cgttgaggag
     2221 tattgctact gctataggta ttttttcagt atgcccgcct tcttcattgg aggggcttat
     2281 aattgacaag gaacaatcca atgtctttat aaacacacca gttaagcttt ctgtcagggg
     2341 ctatgacact tatttcaatc cattcgcggt ggaccagagc aagattaaat ggagtgtgtc
     2401 cggtattgaa ggaagctttg aagacagcac attccatcct aaaacttccg gctttggcac
     2461 aataactgcc tcaatagacg gcgtgacctc cagtaccgga ataatggttc tggacaaacc
     2521 aaaccggctt attctaagtg acaaagagct ttacctgacc gtcggatcat cccgcacctt
     2581 aagtgttaaa gctaccgaca gcaacggtta tacctcaaat gtcaaccctg aaaatatcac
     2641 ctggaccctt gagggagacg ttggaaagct tgaaaacaac accttcaccg ccaccaaaac
     2701 cggtgtaggc tatattaagg catcctgggg ggatgctcaa gcttactgtg ctgtatcggt
     2761 tgtggaagaa accctgcact ctttagacaa gtttgaaaaa tggaacggat tattttcatc
     2821 tgcacccgca aaccttcccg gaagctatga gttatccaac gaacaaaaaa agagcggaaa
     2881 atactcagga aaactgactt atgatttttc atatttagag ggaacaagag ctgcttacgt
     2941 ggtgtttgac aatgatggaa tcaaccttgg tgaaaatacc accgaattga acatgtgggt
     3001 ttacaacccc cgtgagaact ccaattggtt aagggctgag gtcgtcgatg ccgcaggcaa
     3061 aaaacatctc attagttttt gcaacggtat ggactggaca ggctggaggc aagtatctgc
     3121 ttcattgagg gacatcgatt cccccaccag gctgacccgg gtgtatcttg cacaagtaaa
     3181 ccctgttccc gaggcaggtt acatttatat agatgactta ggctttaagc agcataaaga
     3241 ctttcccgaa atcgacaaag acacggttcc tgaggatgtt ttcgctccgg acgcagccaa
     3301 caaagtaacc gaacttgccg acgattccat aaggttctcc gtaattgcca acaccatcga
     3361 aaaacctcag aacatgctgc aaaagcttat gaacctgcgt tttgcggaag gagtcaatgc
     3421 cgatgacaca ataaaggatc ctcaaataat ttcaaatata aagtcttacg actgttttga
     3481 tgcgggcaaa agccggttta tcgttctcga tacatcggac aaaagcctga gaacaagctc
     3541 ctccggacaa tggcaatggt tttcagaaaa actcgaatcc ttttccgggg ataatctctt
     3601 catatttatg aagaatcctc cgtcaacctt tagcgactca ttggaggcta atctattcaa
     3661 aaaattattg gttgagaaaa aggaaaaaac cggcaagaat atatgggtat tctataacgg
     3721 aagtacaaac acatacaccc cggaaaacgg aatccggtat ttcagcctgg cacaagtgag
     3781 tacaacgggt ctgacccccg aaaatgcaat agatgccaaa tatattgagg taactgcaac
     3841 gggcacagat gtaagctatc aatacaagtc aatgtttgaa taggctttca taatgtgaaa
     3901 taatattccg aaacttaaga tttgcaagaa cagagcctgt aaatacttcg cagggtccgg
     3961 caaaactcac ttcgtgagaa tggatgcccg cccttcgtgc gttgtattaa caggctcttc
     4021 gatttgctta cgccatattt cttcggtgtc tttctttact tttattaatt gcaccctgat
     4081 attccgggta cattaatccc ctttactgct cacatcccaa caatttcttc aaataagcct
     4141 caaaatcatc cctgaggtct tccctcttca aagcaaactc aacagtagcc tgcaggaatc
     4201 ccagcttatt tcccacatca taccgctttc cgacaaagtc atacgcgtac attgcttcat
     4261 gctgaataag gcttttaagt gcgtcggtta gctgaatctc tccgcccttg ccgggtttga
     4321 cattctcaag gtgctcaaat atttgcgggg ttataatgta tcgtccaaga atagctacat
     4381 ttgacggtgc attttctctg tccggctttt ccacaacatc cttaacctta tataccctgt
     4441 cgtccaccgg cttgccgcct actattccgt acttggacac atcctcctca gaaaccggct
     4501 gtactcccaa caccgttgtc ttgtactcat tgtaaacatc aatgagctgt ttgatgcacg
     4561 gaacctcaga gtctacaata tcgtcaccca acagcaccgc aaaaggctca ttgccgataa
     4621 aggattttgc acaataaatg gcatgtccca agcccttagc ttccttctgc cttatataat
     4681 gtatatttgc aagatttgaa atatcctgta cttcttttaa aagctgatct ttgcctttat
     4741 ttttaagttc ctgctccagt tcataagact tgtcaaaatg gtcttctatt gcccttttgc
     4801 ttcttcctga aatgattatt atgtcttcaa taccggacat aattgcttct tcgacaatgt
     4861 actgaatggt gggtttgtca acaataggaa gcatttcttt cggctgagcc tttgtagcag
     4921 gcaaaaatct ggtgcccaga cctgcagctg gaattatggc ttttctaact ttcaatctct
     4981 tcacaccctt cttaattgtt agcttgtact acttctattt taccagagaa aactgctctt
     5041 tccaaccggt tttgacatta ttaatattct aatgtacata attattattt tctggtagta
     5101 ttatatataa ggtatttgca ataaaaggtg ggaattgaat gttcgccgga tttattaaaa
     5161 aaaataccgg ggtgttaaaa ataatacatg aagtttacat aaggtcaaag gatgatgaag
     5221 tgcctgcatt ggcagcacag ctgacctatt atttcattct ttcccttttc ccttttttaa
     5281 ttttcataat cacactggta agctatactc ctattgcaag cgaaagggct ttgggcagta
     5341 tttccgaaat gctccctcca ccggtttata atctggtcct gaatgtgata agggaagtaa
     5401 ccctctcccc cagaaaaacc ttcctgtcct tcggtatgat tactgcatta tgggcttctt
     5461 ccaatggaat gaatgccgtc attagaggtc ttaacaaggc gtacgacacg aaagaaacac
     5521 gcccgtactg gaagataaga gggctttccg tggtatccac agccgccctt gccctggcca
     5581 ttataatctc attgggcctt ctggtttttg gcgaaaccat gggggagcaa ctgttcgagt
     5641 tcacggtttt taagacagcc tggacagcat tgagactctg tttttcattt cttttcatga
     5701 ttatggtgtt cagccttctt tacatgctcg tacccaaccg ccgc
//
LOCUS       DAOOJC010000111         5732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_11349_length_5732_cov_4.039105, whole genome shotgun sequence.
ACCESSION   DAOOJC010000111 DAOOJC010000000
VERSION     DAOOJC010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11349_length_5732_cov_4.039105"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PLA01_04380"
     CDS             complement(<1..452)
                     /locus_tag="PLA01_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPD00570.1"
                     /translation="MKLTGSQILIECLKEQGVDTIFGFPGGAVLNIYDALYKAKDEIR
                     HILTSHEQGAAHAADGYARATGKVGVCIATSGPGATNLVTGIATAYMDSVPMVAITGQ
                     VPTTLLGKDSFQEVDITGITMPITKHNFIVKEVEKLADIVRKAFYIAK"
     gene            complement(533..2194)
                     /gene="ilvD"
                     /locus_tag="PLA01_04385"
     CDS             complement(533..2194)
                     /gene="ilvD"
                     /locus_tag="PLA01_04385"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPD00571.1"
                     /translation="MKSDVVKKGIEKAPHRALFKAMGYTDEEIQRPLIGVVNAKNEII
                     PGHIHLDKIAEAVKAGIRMAGGTPVEFGAIGVCDGIAMGHVGMKYSLATRELIADSCE
                     AMGLAHSFDGMVFIPNCDKIVPGMLMAAARINVPSVVVSGGPMLSIQRNGESLDLNSV
                     FEAVGAYKAGKMTEEEVWEYEECACPGCGSCSGMFTANSMNCLTEVLGLGLPGNGTIP
                     AVYAERIRLAKKAGMKVMELVEKDIKPSDILTPKAFENALTVDMALGCSTNSILHLPA
                     IAHEVGVEINLDIVNEISSRVPNLCKLAPAGHHHVQDLYAAGGVQAVMKELSKKNLLH
                     LDLMTATGKTVGENIEKAEVKDREVIRSIDNSYSTSGGIAVLRGNLAPDGAVVKRSAV
                     APEMLVHSGPARVFDSEDEAINAIYNGKINKGDVVIIRYEGPKGGPGMREMLSPTSAI
                     AGMGLDKHVALITDGRFSGATRGASIGHVSPEAMEGGNIAIVREGDIINIDIPNGKLN
                     VEISDDEAEKRFKEWKAPEPRIKKGYLGRYAKLVTSASTGAVLKG"
     gene            complement(2623..3195)
                     /locus_tag="PLA01_04390"
     CDS             complement(2623..3195)
                     /locus_tag="PLA01_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00572.1"
                     /translation="MSNQLPFTISLLSSEYLSLLSNFRCGNEEIDRYIKEDALNDKES
                     GKGVTYLVLNNEKNKLIAYYTLAATSVIYSETKDITAKRNIEEVKIRGFPAIEIKMFA
                     VSKSYQNYTYYSKEFGPKILSDMILGAVIGDIYNYATERLGIRMIILYSTEEGFKFYK
                     RNNFEELESYIPLYSEYTKDCIPMYLRLFD"
     gene            complement(3188..3364)
                     /locus_tag="PLA01_04395"
     CDS             complement(3188..3364)
                     /locus_tag="PLA01_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00573.1"
                     /translation="MALLSRICDRPIVIDKDKSKQFLEDSKKNTIKPEFLSECKKLSS
                     MFKRENSGYIKDNV"
     gene            complement(3774..4493)
                     /locus_tag="PLA01_04400"
     CDS             complement(3774..4493)
                     /locus_tag="PLA01_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPD00574.1"
                     /translation="MNWIDIVVIAIIVGLGVIGLKKGLVYSLFKFVSFFLSAFLAIKL
                     YPLVAKVLAGTELYTSIKAAIFKRLMLQQQAAMPGLNESTQVTAKSVIEGLNLPGFVK
                     DMVANSVVKSIPNISELVNVSGILDSVSDVLAHVIINIISLVVLFIVIKIGLFIVESM
                     ISGVTDLPVLKQADKFGGFALGALEGVLVVYAAFAIMMMFNTVPLFQGFFDAVENSAL
                     AGVFYQNNFILDWMFPKNVIV"
     gene            complement(4512..>5732)
                     /locus_tag="PLA01_04405"
     CDS             complement(4512..>5732)
                     /locus_tag="PLA01_04405"
                     /inference="COORDINATES: protein motif:HMM:NF039834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HPD00575.1"
                     /translation="KRKAWSEDKVDNPEIRPTTQKPSSRMSILLFFILLVTVICTAAV
                     VYLNSKGIDIKEISIKDVIENRFFIGNKDSYEIECKEIEYGTNVQQEFVVHKDFIVRC
                     TKSGIEYFDKNGEEKWLFPVTLNKPYAKSSGKYLLVADLETKDIYVFSGKEKIWEDRL
                     GYSILSADINSNGYVTVVHRSDRNKSAVSVYNREGAFLFTKIKGEAFVISAGVSQDTK
                     NVFVNSVDYSGINANTGLDLYSISGGDIKGTKFENTILASLWFLNDATIAAAGDSEIF
                     LLRKDLEVKWRQTVSGEVMSSNIMKNKYAVFAFSNKENSGILNTDKSNVLIVDENGRK
                     VSDYKLSQDIVNISCGESLIAVNTGKNIYFIDVAGQLRGSVNSEKDIKNVQFFNDREA
                     LAVTRDGIIVIKMK"
BASE COUNT         1651 a         1408 c          866 g         1807 t
ORIGIN      
        1 tcttttgcta tatagaaggc ctttcgtaca atgtccgcaa gcttttctac ttctttcact
       61 ataaaattgt gcttggttat gggcatggtt atgccggtta tatcaacttc ctggaaggaa
      121 tccttgccga gaagtgttgt cggcacctgt cctgtgatcg caaccatcgg aacagaatcc
      181 atgtatgcgg ttgctattcc tgtgaccaga tttgtcgctc cgggtcccga tgtggcaatg
      241 cacaccccta ccttccccgt tgccctagca taaccgtctg ccgcatgagc cgccccctgc
      301 tcatgagatg tcagtatatg gcgtatttca tccttggcct tgtacagtgc gtcatatata
      361 ttcaataccg ctccgcccgg aaaaccgaaa attgtatcaa cgccttgctc cttaaggcat
      421 tctatcaata tttgtgaacc ggttaacttc attttcatta cctcccaatc atttctcaaa
      481 caggcatcca tccaacacca ttgcacccga tcattttaag atgaacaccc tgtcaaccct
      541 ttaagaccgc tccggtactt gctgaagtaa caagcttagc ataccttcca agatagccct
      601 tctttattct cggctccgga gccttccact ccttgaacct cttttctgcc tcatcgtcgg
      661 atatctccac attaagcttg ccgttaggta tatctatatt aattatgtcc ccttccctta
      721 caattgctat gttaccgcct tccattgcct caggagaaac atgtcctatg gaagcccccc
      781 tggtagcacc ggaaaaacgt ccgtcggtaa taagggcaac atgcttgtca aggcccattc
      841 ccgctatcgc agaagtaggt gaaagcatct ctctcatacc cgggccgccc tttgggccct
      901 catagcggat tattaccaca tcacctttat ttattttgcc gttatatatt gcatttatag
      961 cctcatcctc ggagtcaaac actctggccg gtccgctgtg aacaagcatt tccggtgcaa
     1021 cggctgaacg ctttacaaca gcaccgtccg gtgcaagatt gccccttaag accgcaatac
     1081 ctccggaggt actataagaa ttgtcaatgc ttcttataac ctctctgtcc ttaacctcag
     1141 ctttttcaat gttctctccc actgtctttc ctgtagcagt catcaaatcc aaatgcagaa
     1201 ggtttttctt tgacagctcc ttcattacag cctggacccc tccggcagca tataaatcct
     1261 gtacatggtg atgcccggcc ggtgccagct tgcaaaggtt gggcaccctg ctgcttattt
     1321 cgttgactat gtccaggttt atttcaactc ctacctcatg ggcaattgcc ggaaggtgta
     1381 aaattgagtt tgtggaacac cccagggcca tatcaacagt caaagcattc tcaaaagcct
     1441 taggtgtcaa aatatccgaa ggtttaatat ccttctccac caattccatt accttcatgc
     1501 ccgccttctt tgccaaacgt atcctctcgg cataaacggc aggaatcgtt ccattacccg
     1561 gcagaccaag ccccaaaacc tccgtaaggc agttcatcga attggcagta aacattcccg
     1621 aacaggaacc gcagcccggg caggcacatt cttcgtattc ccatacttct tcctcagtca
     1681 ttttacctgc cttataagct cctactgcct caaaaacact gttcaaatca aggctttccc
     1741 cattgcgttg aattgaaagc atgggtccac cgcttaccac caccgaagga acattgatcc
     1801 ttgcggcagc catcagcata cccggtacta ttttgtcgca gttggggata aataccatgc
     1861 cgtcaaaact gtgggccaat cccatagctt cacaggaatc ggcaatgagt tcccttgttg
     1921 ccagggagta tttcattccc acatgcccca tggctatacc gtcgcagact ccaattgccc
     1981 caaactcaac cggagttccg cctgccattc ttatgcccgc ctttaccgct tcggcgatct
     2041 tgtccaaatg tatatgtccc ggaattatct catttttagc gttaacaact ccaataagag
     2101 gtctttggat ttcttcgtcc gtatatccca tggccttaaa cagagctctg tgaggagcct
     2161 tctctatacc cttttttact acatcacttt tcatataacc ttcccctctt tccaaatata
     2221 acgatgttaa tattttatat ttcccaaaag gatacttttt acataattat ttaaatgcat
     2281 atattaataa aacctctact taaaacaata gccgggttcg cccggcttac ttattgtttt
     2341 tgcttattaa ttattttact actatagagc ttcaattgtc cagtgtttta aaaaaaaagc
     2401 acaaatcgtt aatttatttc actggaaaat acaattttcg ttaatcggtg atagccgaat
     2461 aaaaaacgaa atataaagga tcaagcaatt attaccactc acacagttat agaaactatg
     2521 aggtcactca atgcagttac ctcacactat tctacttcac aacacttatt gcataataaa
     2581 aacacaagta aaaacctgtg ttttaacatt agcaaaatat atttaatcaa ataaccttaa
     2641 atacattgga atacaatctt ttgtatattc actatataac ggtatgtagc tttccaattc
     2701 ttcaaaatta tttcttttat aaaatttaaa cccttcttct gtagaataca aaattatcat
     2761 acgaattccc agacgctctg ttgcataatt atatatatcg ccaattacag cacccaaaat
     2821 catatcacta agaattttag gaccaaattc tttactataa tatgtataat tctgatacga
     2881 tttgctaact gcaaacattt ttatttctat ggcaggaaag cctcttattt ttacttcttc
     2941 gatatttctt tttgctgtaa tatctttagt ttcagaatat attacagatg tagctgccaa
     3001 tgtataataa gcaatcaatt tattcttttc gttattaaga actagataag taacaccttt
     3061 acctgattcc ttatcattta aagcatcttc tttaatatat ctatcaattt cctcgttacc
     3121 acagcgaaaa ttacttaata gacttaaata ctcgctactc aacaaggaaa tagtaaacgg
     3181 taattgatta gacattgtcc tttatgtaac ctgaattttc tcttttaaac attgatgata
     3241 gttttttgca ttcagataag aactcaggtt taatagtatt ctttttgctg tcctctagaa
     3301 actgttttga tttatcttta tcaattacaa taggtctatc acaaattctt gatagcaatg
     3361 ccatattatt acctcctttt ctaaatacac ctttaatttt ccgagataat cgaaagccct
     3421 ttcgcataca gtacctgcct ttgattaaat tattcaacaa aatcttatgt ttaatcaaag
     3481 gtttttatac attttccaag aaattttcac tttaatattc tatacacgaa tagacataac
     3541 aatttgtcac atccctataa ctaattatac catagcaaat tatttacgtc aattttttcc
     3601 tatttactgt taatttaaag ccattttaaa caagtatcat gactaaaaaa accatataca
     3661 ataatgtgtg ttttgctcga ccccgaggag tatttacagg ctctggactt gcggaactta
     3721 agtttctgga acacttttct tcacatcttt ggtgcacact catggataat tgactatact
     3781 atgacattct tcgggaacat ccaatccaat ataaaattgt tttgataaaa aacacccgcc
     3841 aaggctgagt tttctacagc atcaaaaaat ccttggaata aaggcacggt gttaaacatc
     3901 atcattattg caaaggcagc atagactaca agcacccctt caagggcacc caaagcaaat
     3961 cctccgaatt tatccgcctg ctttaaaacc gggagatcgg taacaccact tatcatgctc
     4021 tcgacaataa acaaaccgat tttaatcaca atgaacaaaa caacaagact tataatatta
     4081 ataatcacat gggccaaaac atcacttacc gaatccaaaa tgcctgatac attaacaagc
     4141 tcggatatat ttggtatgct tttaactacg ctgtttgcaa ccatatcctt aacgaacccc
     4201 ggcagattga gcccttcaat caccgatttg gctgtaacct gtgtactttc attcaatccc
     4261 ggcattgcag cttgctgctg aagcatgagc cttttaaaaa tagctgcctt aatcgaggta
     4321 tacaattctg ttccggccaa aacctttgca accaaaggat agagcttaat agctaaaaat
     4381 gccgataaaa aaaacgatac aaacttgaaa agggaataaa ccagaccctt tttaagcccg
     4441 ataaccccta agcctacaat aattgctatc accacaatat caatccagtt catatttacc
     4501 cccttgtttt actacttcat ttttataact ataataccgt cccttgtgac tgccaaagcc
     4561 tctctgtcgt taaaaaactg tacattttta atatctttct cagagttaac acttcccctt
     4621 agctgtcctg ccacgtcaat aaaataaatg tttttaccgg tgttaaccgc aataaggctt
     4681 tcaccgcaag aaatattaac tatatcctga cttaatttat agtcggaaac cttccttcca
     4741 ttttcatcca caatcagcac attggattta tccgtgttaa gaatccccga attttcttta
     4801 ttgctgaatg caaacacagc atacttgttc ttcattatgt ttgaactcat gacttcaccg
     4861 ctaaccgtct gcctccactt tacctccagg tcctttctca gcaagaaaat ctctgaatca
     4921 cctgctgctg caatagtggc atcgtttaga aaccacaggg aagccagaat ggtattttca
     4981 aattttgtcc ctttgatgtc tcctcccgaa atggaatata agtccaatcc cgtattggcg
     5041 tttatgccgg aataatcaac ggaatttaca aaaacgtttt ttgtatcctg agacaccccc
     5101 gccgaaatca caaaggcctc accctttatt tttgtaaaca ggaatgctcc ctcccggtta
     5161 tacaccgaaa ccgccgattt attccggtca cttctatgca caaccgttac atatccgtta
     5221 ctattgatat cagcacttaa tatgctgtat cccagcctgt cttcccaaat tttctccttg
     5281 ccgctgaaaa cgtaaatatc cttggtctcc aaatccgcta caagaagata tttcccggaa
     5341 gatttggcat agggcttatt gagggtaacc gggaaaagcc acttttcttc accgttttta
     5401 tcaaaatact ctatccctga cttcgtacat ctgacgataa aatccttgtg aacaacaaat
     5461 tcctgctgca cattagtccc atattctatc tctttgcact caatctcata actgtcttta
     5521 ttgccgataa aaaaacggtt ttctatcacg tctttaatac ttatttcttt tatgtctata
     5581 cctttgctgt tgagataaac aactgcagcc gtacatatga ctgtaacaag aagtataaaa
     5641 aaaagaagta tactcatcct gctcgatggt ttttgtgttg ttggcctgat ctcagggtta
     5701 tccaccttat cctcactcca tgcttttctt tt
//
LOCUS       DAOOJC010000112         5718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_11377_length_5718_cov_3.685679, whole genome shotgun sequence.
ACCESSION   DAOOJC010000112 DAOOJC010000000
VERSION     DAOOJC010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11377_length_5718_cov_3.685679"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..1414
                     /locus_tag="PLA01_04410"
     CDS             8..1414
                     /locus_tag="PLA01_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD00576.1"
                     /translation="MNLKELVLNFEVLAVYRNLLEDKVIKKLYGLISYLDGDKPELDG
                     FIRLYNEFFFQLTVSNSTGTFKDYIIEKLIFDETPFSLLAEGVCFEDIDDHLIQAVSR
                     DLDSLYAISCLTSNEIKKLAVDRICSCDIERNVSDEFPDMTRKNRPPVLLVEKLPSWE
                     FQKKPDSNHKVFSIQYRKMIEILHSCSRWGEKVKELADFYYRNGSGMFARYGAFVWES
                     IEGKSCLRGIEVPDPIRLSDFIGYEQERLEVIENTEKFLKGLPANNVLLYGDRGTGKS
                     STVKAIANEYREMGLRIIEIPRKHLVDFPAVIRQIKGRKCRFIIFIDDLAFEDSEESY
                     TVLKSVLEGGVENRPDNVLIYATSNRRHLIKEKFSDRAGFRSDNPDDEIRAQDTMQEK
                     LSLSERFGITVIFSSPDKKRYLEIVEGLAVQRGIHIDRDRLQKEAMKWELMYNGRSAR
                     TAKQFVDWLEGNERLGNE"
     gene            complement(1424..2056)
                     /locus_tag="PLA01_04415"
     CDS             complement(1424..2056)
                     /locus_tag="PLA01_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00577.1"
                     /translation="MKDIDLAVSKIQAGEEKRSKKNIKPRDKEITAFHEAGHALVAKL
                     LSGVTVSKVTIIPTTKGAGGYTLFVPDEKMFSTKKDLLNDIAIFLGGRAAEEIVFGEN
                     EVTNGASDDLRKATQIAVDMVKNYGMSQTIGLVSFLDAGIDSPSGKNMNAVEQEVKDI
                     IEGVYKETKELLKNNSDCLRNIASLLLERETIYRKDIDNIVETTMFKRDE"
     gene            complement(2057..2707)
                     /locus_tag="PLA01_04420"
                     /pseudo
     CDS             complement(2057..2707)
                     /locus_tag="PLA01_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            3865..5073
                     /locus_tag="PLA01_04425"
     CDS             3865..5073
                     /locus_tag="PLA01_04425"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HPD00578.1"
                     /translation="MSKIKMRVPLVEIDGDEMTRILWKMIKENLLEPYIELNTKYYDL
                     GLVNRDRTDDQVTIDSANDIKKYGVGVKCATITPNAQRVEEYNLKKMWKSPNGTIRAI
                     LDGTVFRSPIIVNSIKPFVKPWKKPISIARHAYGDIYKNIEHYVDGPGKAEIVFTSES
                     GEVTRKTIHDFDGPGVIMGMHNTDKSIKSFARACFNYALDMNQDLWFSTKDTISKTYD
                     HRFKDIFQEIYDNEYKEKFEAKNLEYFYTLIDDVVARIVRSEGGMVWACKNYDGDVMS
                     DMVASAFGSLAMMTSVLVSPDGKYEYEAAHGTVTRHYYKHLKGEETSTNSMATLFAWT
                     GALRKRGELDGINELIDFANKLEKASIQTIENGVMTKDLAQLSEVPEKKIVNSEGFLL
                     EIRKTLESME"
     gene            5142..>5718
                     /gene="truA"
                     /locus_tag="PLA01_04430"
     CDS             5142..>5718
                     /gene="truA"
                     /locus_tag="PLA01_04430"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPD00579.1"
                     /translation="MRNIKMIIQYDGSRYYGWQRLGDTDKTIQGKIEGTLKRMTDEDI
                     EIVGAGRTDAGVHAINQVANFHTGCDKPIEYMLDFCNRYLPKDIVISDMCEAEERFHA
                     RYNAKAKKYLYRIWNEKYHNPFFGKYSYHISREMNIDLMRKAGNILVGEHDFTSFTTL
                     KSKKKSKVRKVYSIDIDKRDGMVEILFYGKGF"
BASE COUNT         1739 a          998 c         1324 g         1657 t
ORIGIN      
        1 gacggagatg aatttgaagg agttagtgct taatttcgaa gtattagctg tttatagaaa
       61 tcttttagaa gataaagtga taaagaaatt gtatggcttg attagttatt tggatggaga
      121 caaacctgaa cttgacgggt ttataaggtt atataatgag tttttctttc agttgactgt
      181 atcaaactca accggtacat ttaaggatta tataatcgag aagctgatat ttgacgaaac
      241 ccctttttca ctgttagcgg agggagtttg ctttgaagat attgatgatc atttgataca
      301 ggcggtttca agggatctgg acagccttta tgccatatca tgtttaactt ccaatgagat
      361 caagaaattg gcagtggata ggatctgcag ttgcgatata gaaaggaatg tatcagatga
      421 gtttcctgat atgacacgga agaaccgtcc ccctgtgttg cttgtggaaa agcttccgtc
      481 atgggagttt cagaaaaaac cggacagcaa tcataaagtt ttttcaattc aataccgaaa
      541 gatgattgaa atccttcatt cgtgcagccg gtggggggaa aaggtgaagg agctagccga
      601 tttttactac cgcaacggct cagggatgtt tgcccgttac ggtgcttttg tatgggagtc
      661 tatagaagga aagtcatgcc ttagaggaat cgaagttccc gatcccatcc ggctatcgga
      721 ttttatcggc tatgagcagg aacgccttga agtaatagag aacacggaaa agtttttgaa
      781 aggattgcct gcaaataatg ttttgctata tggagacagg ggaacgggga aatcgtcaac
      841 cgttaaggcc attgccaacg agtatcggga gatgggactt agaatcattg aaattccacg
      901 aaagcatctt gtggattttc cggcagtaat aaggcagatt aaaggaagaa agtgccgatt
      961 cattattttt atagatgatc tggcttttga ggatagtgaa gagagctata cggttcttaa
     1021 atcggtactg gagggaggag tggaaaacag accggacaat gttctgattt atgccacttc
     1081 caaccgtcgt catcttataa aggaaaaatt cagtgacaga gcaggcttca ggtccgacaa
     1141 tcccgacgat gaaatcaggg ctcaggatac tatgcaggaa aagctctccc tttcagaaag
     1201 gtttggtatc acggttattt tttcatcacc ggataaaaaa cggtatctgg agattgtcga
     1261 aggtcttgcg gtacaaagag gcatacacat tgacagggac aggctccaaa aagaggcgat
     1321 gaaatgggag cttatgtaca atggtcgctc tgcgaggact gcaaagcagt ttgttgattg
     1381 gctggaagga aatgagagat tgggtaacga gtaaaccgga agattactcg tctctcttga
     1441 acattgttgt ttcaacgatg ttatcgatgt cttttctgta tatggtttcc ctttcaagaa
     1501 gaagggaagc tatatttctg agacagtcgg aattgttttt caacagctcc tttgtttcct
     1561 tatagacgcc ttcgattata tctttgacct cctgctctac tgcattcata tttttaccgg
     1621 atggagaatc gattccggcg tccagaaaac tgaccagacc gatggtttga ctcattccgt
     1681 agttcttaac catatctacg gcaatctgag ttgcttttct aaggtcgtcg ctggctccat
     1741 ttgtaacctc gttttcaccg aatacaattt cttctgcagc tcttccgccc agaaatattg
     1801 cgatatcatt caaaagatct tttttggttg aaaacatttt ttcatcgggt acaaaaagag
     1861 tataaccacc ggcacccttg gtggttggaa taattgtcac tttggatacg gttacacctg
     1921 aaagaagctt tgccaccagt gcgtgaccgg cttcatgaaa tgcagttatt tccttatctc
     1981 tgggcttgat gtttttcttg cttctttttt cttcgccggc ttgtattttg ctgacagcaa
     2041 ggtctatatc cttcatggtg atgaaggggt gaccgtttct tatggcataa atcccggctt
     2101 cattcatcac attggccaga tcggcaccgg acatgtatac tgtcattcgg gcaatttctt
     2161 taaggtcgac atcacttgcc agcggtttgt tttttgcatg aagtgcaaga atggaggttc
     2221 tggcttcgac atccggcaag gctacctgaa tttgcctgtc aaatcttccc gaacggagga
     2281 gggcagggtc cagcatatca agcctgtttg ttgcagctat tactataata ccttcactgc
     2341 cggaaaaacc gtctatttcg acgagaatct gatttaaggt cctgtccttt tcgtctcctg
     2401 aacccgaact gttattgctg cttcttttgc ccaatgcatc tatttagtct atgaaaatta
     2461 tgcagggact gattttacgt gcgaactgga aaagttgcct tattcgtgaa gcaccaacgc
     2521 ccacaaattt ttcaacgaag tcggagcctg agacggctac aaaaggcacc ccggctgtac
     2581 cggctaaagc cttggccaga agtgtctttc ctgttcctgg aggaccgaaa aggacagtcc
     2641 ctttcggggg aataactcca tactttaaaa atttatcggg attggttaaa aaatcaacca
     2701 gttccatcat gttttgcttt gcttcttcat ttcctgccac atcttcaaag gttatattgc
     2761 atttttttat ggtgtcaaat tttgaatcct taccggaata ctgtttttta ataagtctgc
     2821 ccattaccaa gccgattaag gcaactgcga aaaccatggg caagacggcg gccagtattt
     2881 tgaccagcat attgggcaag gtttcaataa agggtttatc ctcaaaagct ataccctgct
     2941 caagaagata ttttttaatg tcagtggact gagggttgtc tgttataaac agtttattgt
     3001 ccttaaacct tccagtgatt tttttggcat tttgactgta gtcaatgatt gataaaacat
     3061 tacctgactc aatagcattt ttgaactccc ggtaggttat agctttaatg ctgctgtttt
     3121 ctgccggtgg tttaatagaa tttgcataga taaaaatagc tgccatgctg attagaaaaa
     3181 ggcatattat agacaggaag gtgagctgcc tcgtccgtga ggatttaaca gctctatagt
     3241 atgctggaat aagtactaaa aagaagtatc ggatgctggg aattagtttg ttgcgaatag
     3301 tgaggtatag tttagagaac aggggagaca ccaccttccg tcaaaactta cgaggtcgtt
     3361 gttatatatt tattttatca gagaagcttc caaatttcca tggaacataa ggactatctg
     3421 atgtctcata ctaaagtctc aattgtaggt agtatgacac agtacagtat gacaaattaa
     3481 aatgatgttg ataagatgaa aatgcatatt cgtcggtaga ttttgaagat gggttgtttg
     3541 aagctgatga ggataacaag atttttgcat ttttattgtt actttttcta caggggggat
     3601 atatgataaa aaagggtgta tgtacgacaa tccgatccaa tttataatta gaaaaatact
     3661 atgttaaatc tttacactcc atattttatc agacttaatt tacttttgtc tacagaggct
     3721 tcatgaattg ggtatgtaag tattacatcc acagttaaaa agaaccttga taatattatg
     3781 aaattaattt aaaataaaga ggagctttga taaaatccta acaaagaata ttaaaacaat
     3841 ttattataga aggagaagag atctatgagc aaaatcaaga tgagagttcc cttggtagaa
     3901 atagatgggg acgaaatgac aagaatatta tggaagatga taaaggaaaa tcttttggag
     3961 ccgtatattg agctcaatac caagtactat gatttgggcc tggtaaacag agacaggaca
     4021 gatgaccagg taactatcga ttcagctaat gacatcaaaa aatatggggt tggggtaaaa
     4081 tgtgctacaa ttacccctaa cgctcaaagg gtagaagagt acaacctcaa gaagatgtgg
     4141 aagagtccga acggtactat cagggcaatt ttagacggta cggttttccg ttcgccgata
     4201 attgtcaata gcattaaacc ctttgtcaag ccatggaaga agcctatctc catcgcaagg
     4261 catgcgtacg gcgatattta caagaatata gaacactatg tagatggccc gggaaaggct
     4321 gagattgtct tcacttcgga gagtggagag gttacaagaa agaccatcca tgattttgat
     4381 ggaccggggg taataatggg tatgcataat accgataaat ccataaagag ttttgcgaga
     4441 gcctgcttta attatgctct ggatatgaat caggatttgt ggttttcaac caaagacacc
     4501 atttctaaaa catatgacca caggtttaaa gatatattcc aggagattta tgacaatgaa
     4561 tataaagaga agtttgaagc caaaaacctt gaatatttct atactctgat agatgatgtg
     4621 gttgcccgta ttgtaaggtc ggaaggtggc atggtctggg catgcaagaa ctatgacggt
     4681 gatgttatgt ccgacatggt ggcttcagct tttggcagcc tggctatgat gacgtctgtt
     4741 ctggtttcac cggatgggaa atatgagtat gaggcagccc acggaacggt tacaaggcac
     4801 tattacaagc accttaaagg cgaggaaacc tctaccaact caatggcgac tttatttgca
     4861 tggacaggtg cgttgagaaa acgtggagaa ttggacggta taaatgagtt gattgacttt
     4921 gccaataaac tggaaaaggc ctcaattcag actatagaga atggtgtaat gacaaaggat
     4981 cttgcacagc tttcagaggt tccggaaaag aagatagtaa attccgaagg gttcttgctg
     5041 gaaatcagaa aaacccttga gagcatggaa taattattca cagttgcccg catgggcagc
     5101 aacatggtgg tggaatccca ttaaattgga gtgtccggat aatgagaaac ataaaaatga
     5161 taatacagta tgacggaagc agatattacg gttggcaaag gttgggagat accgataaga
     5221 caatacaggg caaaatagag ggcaccttaa aaagaatgac tgatgaagac attgaaattg
     5281 tgggagcagg caggacagat gcaggagttc atgcaataaa ccaggttgcc aacttccata
     5341 ctggatgcga caagcccatc gagtatatgc ttgacttttg caacaggtat ctgccgaagg
     5401 atattgtaat ttcagatatg tgtgaagcag aggaacgttt tcatgccaga tacaatgcaa
     5461 aggcaaagaa atacctttat agaatatgga acgaaaaata ccataatccg tttttcggga
     5521 aatactccta tcatatttcc cgggaaatga atatcgattt aatgagaaaa gcgggaaata
     5581 ttttggtggg agagcatgat tttacaagct ttacaacatt aaaaagcaaa aagaagtcca
     5641 aggtcaggaa ggtttattca attgacatcg ataagcggga tggtatggtg gaaatattgt
     5701 tttatggaaa aggatttc
//
LOCUS       DAOOJC010000113         5668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_11478_length_5668_cov_4.323356, whole genome shotgun sequence.
ACCESSION   DAOOJC010000113 DAOOJC010000000
VERSION     DAOOJC010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11478_length_5668_cov_4.323356"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..214
                     /locus_tag="PLA01_04435"
     CDS             17..214
                     /locus_tag="PLA01_04435"
                     /inference="COORDINATES: protein motif:HMM:NF025298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HPD00580.1"
                     /translation="MEKVGEYLENTWKKALSAEPPEEVKVKILPVYKGNSKRPERFII
                     EYNIGDDIFKKKNLKNASGGK"
     gene            216..683
                     /locus_tag="PLA01_04440"
     CDS             216..683
                     /locus_tag="PLA01_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HPD00581.1"
                     /translation="MEKKLNEIYGKIAGTLNETIPEDWDKVIMYGEVLEGASETYFNY
                     YPTGSNKSVYSHDIPHLFDISEDEYNQLWLRLIDYLEELHDIFIQNEQEPWTNLTFIL
                     ESNGTFKIDYDYTDLSDADDVERHMIWNYKYLGIIPQKERYKKIVEEYIKSLG"
     gene            complement(771..2045)
                     /gene="aroA"
                     /locus_tag="PLA01_04445"
     CDS             complement(771..2045)
                     /gene="aroA"
                     /locus_tag="PLA01_04445"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPD00582.1"
                     /translation="MLIEQRGSLRGEVKVPGDKSISHRAILFGSLAKGTTEIDGFMMG
                     EDCLSTIDCFRKMQVGIEILPNNKIRIQGNGLYGLKPPGTQLNAGRSGTALRLLLGVL
                     SGQPFSSVITRNESALRKPVGKVVDPLREMGANISGRENGNLCPLSITPSKLSGKTHS
                     ISILDTYIKSPLLIAGLYADGETTVIEAAKSRDHSELMLNYFGADIRVNGLEVTSHRV
                     ENLFAQQIQIPGDISIAAYFLTAGLIVPNSDIVIKDVGINPTRAGILEVYKSMGANIE
                     LLNQRTVNNERIADIRVVSSSLKGTTIEGDIIPRLIDEIPIITVAAAMAEGTTTIKSL
                     KGFKTKESGKLKKMLTELAKLGANVEETDDGAIIQGKGALKGTIVEGHNDAAITMSLC
                     IAGLAAAEETTIRKTQILDIVYPDFIPVLNRL"
     gene            complement(2153..3250)
                     /locus_tag="PLA01_04450"
     CDS             complement(2153..3250)
                     /locus_tag="PLA01_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HPD00583.1"
                     /translation="MQDKKIVIIGLGLIGGSIAKALKERLNITCITAVDNNESSIRQA
                     ISEGYIQEGFTELNDSVYNSDIIFLCTPVKYTLEYLTLLCGNVKESCILTDTASTKKE
                     IIDYINSLDNPPCFIGGHPMAGTEKAGFASSFSHLFENAYYILSPSKNSPKEALELLT
                     EIVEGIGAIPVKLDAKEHDIITATISHVPHVIASALVNLVKQSDSPERKMQVLAAGGF
                     KDITRIASSNPQIWESIILSNKQEVKETLNKFIKTISTFIEYIDNNNSGNIYSFFESA
                     KNYRNTFSNNRVGLINPQYELIVDVVDKPGIIGEIATLLGKNGINIKNINVSNSREFE
                     QGCLRITLPDSESEKAAYDMLSDRDYKVFKT"
     gene            complement(3240..4265)
                     /gene="aroF"
                     /locus_tag="PLA01_04455"
     CDS             complement(3240..4265)
                     /gene="aroF"
                     /locus_tag="PLA01_04455"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPD00584.1"
                     /translation="MIIVMKSNSNENDIQEISKVLGGLGLGVHISKGSEKTIIGVIGD
                     KRKLADVPFELMNGVERLVPIVESYKLASRTFQPEPSIVEVGGVKIGGKELVVMAGPC
                     AVESREQILTSAAAVKKAGAQFLRGGAFKPRTSPYAFQGLEEEGLKLLKEAKEATGLL
                     VISEVTSEWAVEIADKYVDMFQVGARNVQNFQLLREIGRSKKPVLFKRGPSTTIDEWL
                     NAAEYIMSEGNYNVVLCERGIRTFETATRNTLDISAVPVIKSLSHLPIIVDPSHAAGK
                     AQYILPLSKAAIAAGADGLIIEVHPNPKCALSDAAQQLPPEDFDNLCKDISKIADIIG
                     REFHYAG"
     gene            5231..>5668
                     /locus_tag="PLA01_04460"
     CDS             5231..>5668
                     /locus_tag="PLA01_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPD00585.1"
                     /translation="MKDLAAGRIRNVCLMAHGGAGKTTLAEAMLFNTGVLERFGRIED
                     GTTTSDYDPEEIKRKSSISTSICPCEWNNHKINVIDTPGYFDFVGEVKQGIRVADSAI
                     ILVSAKSGVAVGTEKAWDYASERKIPKMFFINKMDDENANFFGV"
BASE COUNT         1738 a         1077 c         1079 g         1774 t
ORIGIN      
        1 accttaacaa aggtgaatgg agaaagttgg agaatacttg gaaaatactt ggaaaaaagc
       61 tttaagtgca gaaccaccag aagaagtaaa agttaaaata ttaccggttt ataaagggaa
      121 ttcgaaacgt cctgaaagat ttataattga atataatata ggtgatgata tatttaaaaa
      181 gaaaaattta aaaaatgctt cagggggtaa atagcatgga aaaaaaatta aatgaaatat
      241 atggcaaaat tgcaggaact ttaaacgaaa ctatccctga agattgggat aaagttatta
      301 tgtatggtga agtattagaa ggtgctagtg aaacatattt taattattat cctacaggaa
      361 gtaataaatc agtatatagt catgatattc cacatttatt tgatataagt gaagacgaat
      421 ataatcaatt atggctacgg ttaatagatt atttagaaga actacatgat atatttatac
      481 aaaacgaaca agaaccatgg acaaatctga catttatatt agaaagtaat gggacattta
      541 aaatcgatta cgattatacg gatttatctg atgcagacga tgttgaacgt catatgattt
      601 ggaattataa atatttaggt attatacccc aaaaagaaag gtataaaaaa attgtcgaag
      661 aatatataaa aagcttaggc taactggata aaaatatgca aaataaaaca aaacgctcta
      721 aaatagctta gagcgttttg ttaaaagcaa aggattcttt cgaacaggaa ttagagcctg
      781 tttaaaacag gaataaaatc cggatatact atatcaagta tttgcgtctt ccttatggtg
      841 gtttcctctg cggcagcaag tcctgctata caaagggaca tcgttattgc tgcatcgttg
      901 tgtccctcca cgattgttcc cttaagtgca ccttttcctt gaattattgc tccgtcatcg
      961 gtttcttcca catttgctcc gagttttgca agttccgtca acattttctt gagtttgccg
     1021 gattcctttg tcttaaagcc tttaagactc ttgatggttg tagtgccctc agccatggcg
     1081 gcggcaacag tgatgatcgg tatttcatcg ataagccttg gaataatatc gccttcaata
     1141 gttgttccct taagggatga agatacaacc ctgatatccg caattctctc gttatttaca
     1201 gtgcgttggt tgagaagttc aatattggca cccatggatt tatacacttc caggataccg
     1261 gctctggtgg ggttaatgcc cacatcttta atcacaatgt cggaattcgg cactatcaga
     1321 cctgcggtta aaaagtatgc agcaattgaa atatctcctg gtatttgaat ctgctgggca
     1381 aatagatttt caaccctgtg ggaggttact tccagaccgt taaccctaat gtctgcacca
     1441 aagtaattta acattagctc agaatggtct cttgatttag ctgcttcaat tactgtggtt
     1501 tcaccgtcag cataaaggcc ggcaatcaaa agcggagatt tgatgtatgt atcgagaatc
     1561 gaaatgctgt gggtttttcc cgaaagtttt gagggcgtta ttgaaagggg acagagattt
     1621 ccgttttctc ttccgctgat gtttgcaccc atttccctca aagggtccac aaccttgcct
     1681 acaggttttc taagggccga ttcatttctt gttattactg aagaaaaggg ttgaccagaa
     1741 agcaccccta aaaggagcct tagggcagta ccggatcttc cggcgtttaa ttgagttccg
     1801 ggaggcttca gaccatagag tccattaccc tgaatcctaa ttttgttgtt gggaaggatc
     1861 tcaataccaa cctgcatttt cctgaaacaa tcaatggtac taagacagtc ctcacccatc
     1921 ataaatccgt caatttcagt agttcctttt gctagagagc cgaaaagtat agccctatgg
     1981 gaaatggatt tatctcccgg cacttttact tcgcctctta gtgaacctct ttgctcaatt
     2041 aacacactca attcccccat ttcataagta tatgttaagc cttttattgt gaaaacttat
     2101 ttcaaacaat tgcatgcaaa ctttaataaa cacacaaaca agctgctgac cgtcaagttt
     2161 taaaaacttt gtaatctctg tccgaaagca tgtcataggc tgctttctca ctttcagaat
     2221 cgggtaaggt tatcctcaga caaccctgct caaactcccg gctgtttgag acatttatgt
     2281 ttttgatgtt tataccgttt ttgccgagga gcgttgctat ttctccaatt attccgggtt
     2341 tgtcgactac gtcgacgatt aattcatatt gagggtttat taagcctacc ctgttgtttg
     2401 agaacgtgtt cctgtagttt ttggccgatt caaaaaagct atagatattg ccggaattat
     2461 tattatcaat atattcaata aatgtgctta ttgttttgat aaatttgttt aaagtttcct
     2521 taacctcttg tttgttgctc aaaataatgc tttcccatat ttgaggattg gatgaagcaa
     2581 tcctcgttat atccttaaaa cctcctgccg ctaaaacctg catctttctt tccggtgaat
     2641 ccgattgctt tacaaggttt actagggcag aagctatcac atggggaaca tgacttattg
     2701 ttgctgtaat aatatcgtgc tcttttgcat caagcttaac aggtattgct ccgattcctt
     2761 caacaatttc tgtcaaaagc tcaagggctt ccttcggaga atttttcgaa ggtgacagaa
     2821 tataatacgc attttcaaaa aggtgcgaaa agctggatgc aaaccctgcc ttttccgtgc
     2881 ctgccattgg atgtccccct ataaagcagg gaggattatc caatgaatta atataatcaa
     2941 taatctcttt tttcgtactt gccgtatctg tcagtataca gctttcttta acattgccgc
     3001 aaagcaaggt taaatactcc aaagtatatt taaccggtgt acaaaggaaa attatatcgg
     3061 aattatacac cgaatcattc aactctgtaa aaccttcttg tatgtatcct tcactaattg
     3121 cctggcggat actggattcg ttattatcca ctgcagttat acaggtaata ttcaaccgtt
     3181 cttttaaggc ctttgcaatt gaaccgccta taagaccaag accaataatg actattttct
     3241 tatcctgcat aatgaaactc tcttcctatt atgtcggcaa ttttactgat atctttacac
     3301 agattatcaa aatcctccgg cggcaattgc tgtgcagcat cggaaagtgc acatttggga
     3361 ttgggatgga cttctataat gagtccgtcg gcacccgctg caattgcagc ttttgaaaga
     3421 ggaagtatat actgtgcttt gcctgctgcg tgacttgggt caactattat gggaagatgg
     3481 ctcaaactct ttatcacagg tactgcactt atatccagag tgtttctggt agctgtttcg
     3541 aaggttctaa tacctctttc acaaagaaca acgttataat tgccttcgct catgatgtat
     3601 tcggcggcat ttaaccattc atctatagtg gttgatggac ctcttttgaa aagaacaggc
     3661 tttttggaac ggccaatctc tcttaaaagc tgaaagtttt gtacgtttct ggctcctacc
     3721 tggaacatgt caacatactt gtcggctatt tcgacagccc attcgctggt aacctcggaa
     3781 ataacaagaa gcccggttgc ttcctttgct tccttaagca gcttgagtcc ttcctcctca
     3841 agtccctgga aagcataagg agaagttctg ggtttgaagg ctcctcccct caaaaactgt
     3901 gcacccgctt ttttaacagc ggcagcggag gttaaaatct gctcgcggct ttcaacggca
     3961 caagggcctg ccattacaac aagttccttt ccgccgattt ttacgccacc cacttcaact
     4021 atgctgggct cgggctgaaa ggttctgctt gcaagcttat aggattcgac aatgggaacc
     4081 agcctctcaa caccgttcat aagctcaaaa ggcacatcgg caagttttct cttgtctccg
     4141 ataacaccaa ttatggtttt ttcggaacct ttggaaatgt gtaccccaag cccaaggcct
     4201 ccgagaacct ttgaaatttc ctgaatgtca ttttcgtttg aatttgattt cattacgata
     4261 atcatacaaa aatcccttcc tttctaacca tactaataat ttgagtcaaa agacttaccg
     4321 taatacaaaa atgctgcctg ccaaaacaat aatcgaccga tgctgcttca acttatgatg
     4381 taccgattat tgaaaaaaat atacccatac taccgtactg taaagcaatc aaccatgttt
     4441 gctttatatt aatgtacaat gataatacac aattgtcaac aaaaataact cttacgggtg
     4501 cattaaataa acaaaattga aagggcattg agcccccatc gctttttggt catttcactt
     4561 cgtgaaatcc cactatgtga agaataataa tatataccca actcttatac aataatatcg
     4621 gtcactctga ttaccttctc cgataaaaaa ttcattttat atttaatttt tcgccttcag
     4681 aataaaggtt attgctattt acatataatg ctaagtgtaa tataatgtgg tttggtatat
     4741 gtggttttat ttcgtccaag cagaaaacct gatttttgtg ctgggattcg gaaagggaaa
     4801 tatctcaata acaacggttt caatgatcga taagtttttt tgcttatggt gagaaaatat
     4861 acatattatc aaaatattgt gaaaaaatat tattatagct aggcaatata agacttacac
     4921 ttcttaatag gcagcaggta tcggttggcc ttttgagcgg tgggcatact tattgcctca
     4981 tagatacact gctgatgaaa gaaaatttat ttgcaaacga aaattttctt taaatttgaa
     5041 gtgttataaa agagggaatt acgaaaataa atcgaataaa taagtgtact ctatataaat
     5101 aactgtcatg tttattgcac ccggtagaca gcacggtcaa cacacaatat ttgttaaact
     5161 cctttatggt tttgaaagtt tatgatatga acttaattta agttcaataa atatacaagg
     5221 gggaattatt atgaaagact tggccgcggg gaggatacgt aacgtatgtc ttatggctca
     5281 tggaggggca ggtaagacaa ctttggctga agcaatgttg tttaatacgg gagtacttga
     5341 aaggtttgga agaattgaag acggtactac cacttcagat tacgatcctg aagaaatcaa
     5401 gagaaagagt tcgataagca cttctatttg tccatgtgaa tggaacaatc acaaaattaa
     5461 tgtaattgat acacccggat attttgactt tgttggagaa gtcaagcagg gaataagagt
     5521 tgcagacagt gcaattatac ttgtttctgc aaaaagcggt gttgctgtcg gtacagaaaa
     5581 agcatgggat tatgccagtg agagaaaaat tccgaaaatg ttctttataa acaaaatgga
     5641 cgatgagaat gccaactttt ttggggta
//
LOCUS       DAOOJC010000114         5555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_11771_length_5555_cov_3.623455, whole genome shotgun sequence.
ACCESSION   DAOOJC010000114 DAOOJC010000000
VERSION     DAOOJC010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11771_length_5555_cov_3.623455"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1673)
                     /locus_tag="PLA01_04465"
     CDS             complement(<1..1673)
                     /locus_tag="PLA01_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPD00586.1"
                     /translation="MKKKGLALLLLSVFIVVSCLSASFVQADASPSIELVFDSLQGKQ
                     GDILVATIKVNNVRNFAGYQVNIIYDRNVFKPVDPETGKDYGSATQLVGGTLLKNQKY
                     GPTPLAVHNLNTGVLNFSNTYIYLENYKKSGEPEESGIIGKIGFKVIEARNTRVKFAE
                     SATMPDSISGTYLFDWDGNTLSGYDVIQPERIDLGFDPYQTMEPTPTPDSTPTPPPTM
                     GGTPLIVDVVPELDDTNGEATTAVDTDKVKDALDEADSSGGIKNIEFTVQKVEGADAY
                     ITELPKEFFTKTDIEYKISISTEFGTLEVPSNMMSHPEMLKEIVQASDIQFVIKSIDR
                     EELSSELKALIGNRPVVDISIAVDGRKLAWNNPDAEFRVSIPYTPSSQELDNHEHIVV
                     LYINDAGAPVSVPSGRYDTAAGCVTFTTTHLSRYAVAYVHKTFTDIGSYSWAKKQIEV
                     LASKGVINGTSDTTYTPGADITRADFMILLVKALGLTASVDSNFDDISKNDYFYEYVG
                     IAKKLGITSGVGNNKFNPREKITRQDMMVLTTNALKIAGKISGTGTASDV"
     gene            complement(1964..3307)
                     /locus_tag="PLA01_04470"
     CDS             complement(1964..3307)
                     /locus_tag="PLA01_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00587.1"
                     /translation="MKKLLCFFVLTIMVMNCSFVFAQTSDEPELNGIAFNGSDLYVAV
                     GFEGVIFMSRDGINWSKIESDTNHTLNDVVWGMNRFIAVGDEGTILTSKNGLNWVQVE
                     SDTNLDLQSVAFNGEQLVAVGNSGTVLVSSNGTEWKRIRTATMESLMSVKWFNGKYMA
                     VGGGMLILSSDDGITWEEIMFDPSSTLAFMDVSWNGSSYAVVGDHISVFVSDDGKAWQ
                     QIDTNKLSNDTGIDYDKCLYSVVWGNDSFLAVGHTGSIISSKDGLEWSGNKQITRSTI
                     RNIIWTGDKYVAVGDQGAILLSYDSSAWEDLRKITSDLKEYNITIGQEQQLKVTLKYP
                     YGSEIDITEKVTFDVDNKDIAFIDENGILKPLGIGQATAKIRYDNKSIEVPIKVSEIM
                     GADEENQDNKVNEPEKPQGSNMGDVVLKVVIVLFILSALMGAIVLYYKKMERYKE"
     gene            complement(3446..3703)
                     /locus_tag="PLA01_04475"
     CDS             complement(3446..3703)
                     /locus_tag="PLA01_04475"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HPD00588.1"
                     /translation="MQPITPKDLGLSTQYAGLPTNLILDGVIIEDALHSVKLSKAWLY
                     HQLSRKNITDIGKIALAQLDTEGNLYVDLKGDEPYYIIPTK"
     gene            complement(3691..4155)
                     /locus_tag="PLA01_04480"
     CDS             complement(3691..4155)
                     /locus_tag="PLA01_04480"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HPD00589.1"
                     /translation="MSIISEVVLRSVISFFVLLLLVRLLGKQQVSQLTHFDYVVGITI
                     GSIASNLSVQTDDNFTATLAGLIVWTVLALLLAVSSMHSAWMRKIVIGKKTIVIENGK
                     ILDSNLNKIRMPLEQLFSELRTQGVFNVADVEFAMFEPSGKISVQKNHNCSP"
     gene            complement(4189..>5555)
                     /locus_tag="PLA01_04485"
     CDS             complement(4189..>5555)
                     /locus_tag="PLA01_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPD00590.1"
                     /translation="DKLILSMGAEPLKPKIEGIDSNKVFVLRNIPDTYKINEYIEAYK
                     PKSAVVVGGGFIGVEMAENLHERGIGVTLVEMVNQLIAPIDYDLACDVHNHIVSKGVK
                     LFLGNSVRSIADKGDSLDIALDSGNINADMVIMAVGVKPETQIAKEAGIAVNERGSII
                     VDEHMRTSETDIYAVGDAVEITDFVTGIKGFVPLAGPANKQGRIAADNVCGIDSIYTG
                     TQGSSIIKVFDLTVASTGINEKTAKHLGLNYDKSYTYSANHASYYPGAKGMSIKTIYE
                     KGTGKILGVQIVGYEGSDKRCDVFATAIRAGMTAFDLTRLELCYAPPYGSAKDPVNMA
                     GYVIENILTNKVKIFHWHDVNNLPRDGSVTLLDIRTVNEYNRGHIKGFVNIPLDSLRS
                     RLTEIDKTKPVYVTCQVGLRGYIAARILSQNGFDVYNLSGGYRLYNSIFGFDNSFFSG
                     NNMC"
BASE COUNT         1626 a         1301 c          936 g         1692 t
ORIGIN      
        1 acatcggagg ctgttcctgt acctgatatt tttcctgcta tcttaagtgc atttgttgta
       61 agaaccatca tatcctgcct tgttattttt tctctcgggt tgaatttgtt gttaccgact
      121 ccggaggtaa tcccaagctt cttggctatt cccacgtatt catagaagta atcgtttttc
      181 gatatatcat caaaattgga atcaacagaa gcagtcaatc caagggcctt taccaaaagg
      241 atcataaagt ccgccctcgt tatgtccgcc cccggtgtat atgtggtatc cgatgttccg
      301 ttaattacac ccttggaggc aagcacctca atttgcttct ttgcccaact gtaggaaccg
      361 atatcggtaa atgtcttatg aacataggct acagcatacc tgcttaaatg agttgtcgta
      421 aatgttacac agccggctgc agtatcatat ctgccactgg gcaccgaaac aggagctcct
      481 gcatcattaa tgtataacac aacaatgtgc tcgtgattgt caagctcctg tgaactgggt
      541 gtatacggta tggaaaccct aaactctgca tcaggattgt tccaagccaa ttttctaccg
      601 tcaaccgcta tgctgatgtc taccaccggt ctattgccaa ttaaagcctt gagctccgaa
      661 ctcaactctt ccctgtctat actttttata acaaactgaa tatccgaagc ctgtacaatt
      721 tccttcaaca tttcaggatg gctcatcata ttggaaggaa cctctaatgt tccaaattca
      781 gtagatatgg atattttgta ctcaatatcg gtttttgtaa aaaactcttt tggaagctca
      841 gtaatatatg catctgcacc ttcaaccttc tgaaccgtaa attctatatt tttaataccg
      901 cccgaactat ctgcttcatc cagtgcgtcc tttactttat cggtatcaac tgcagtcgtt
      961 gcttcgccgt ttgtgtcatc caactccggt actacatcaa ctatcaatgg agtacccccc
     1021 attgtgggag ggggagtcgg agtagaatct ggtgtaggcg tcggttccat ggtttgatat
     1081 gggtcaaagc ccaaatcaat tctttccggt tgaattacat catacccgct gagggtgttt
     1141 ccatcccaat caaacaaata cgtgcctgaa atcgaatccg gcatggttgc actttccgca
     1201 aacttaaccc gtgtgttgcg tgcctcaatt accttaaatc ctattttccc gattattccg
     1261 gattcttccg gttctcctga ttttttatag ttttccaaat aaatatatgt attgctgaag
     1321 ttaagtactc ccgtgtttaa attgtgaact gccaagggtg taggtccgta cttttgattc
     1381 ttcagcaaag ttccgccaac caattgtgtt gcagaaccat agtccttccc cgtttctggg
     1441 tctacaggct taaaaacatt tcggtcatat ataatgttaa cctgatatcc ggcaaaattc
     1501 cttacattat ttactttaat tgtcgcaacc aatatatctc cctgcttccc ttgcaagctg
     1561 tcaaatacca gttcaatact tggtgatgca tctgcctgta caaaactcgc tgaaaggcac
     1621 gatacaacaa taaaaaccga caacaataac aaagctaatc cttttttctt catggttttt
     1681 aaaacctcct aataaaatgt gtttaactct gcaaaaaaac ggcgaactac cttaacaaaa
     1741 gactgatagc tgaagattcc tcctaacaat atttcaaatc gtaaaaacac tgttaattca
     1801 tcttcaaaat acgacaaaca ctaacttata cataattaga ttatagcatt gcaagaatac
     1861 aatgtaaatc atgaaaatta taccctgtgt ggccaagaca tgaacccgtc ttttgtcaac
     1921 actcttacaa gagagtttta taaaatggcg gaaaaattag agtctattcc ttgtaccgtt
     1981 ccatcttctt gtaatataaa acaatcgcac ccataagagc tgaaagaata aacagcacta
     2041 taacaacttt cagaaccaca tcccccatat ttgaaccctg gggtttttcc ggttcattga
     2101 ctttattgtc ctgattttcc tcatctgcac ccattatttc acttaccttt atgggaacct
     2161 ctattgattt attgtcatac cgtatctttg ctgtcgcctg tccgataccc aagggcttta
     2221 gaataccgtt ttcgtcaata aaggctatat ctttgttatc aacatcgaaa gtcactttct
     2281 ctgttatatc aatttcgctt ccatagggat atttcagggt cactttaagt tgctgttcct
     2341 gccctattgt aatattgtat tccttcaaat ccgatgtaat ttttcttaga tcttcccaag
     2401 cagaactgtc ataggacaga agaatcgcac cttgatcccc caccgcaaca tacttgtccc
     2461 cggtccatat tatattccta atagtgcttc gtgttatctg cttattaccg gaccattcca
     2521 gaccatcttt agatgagatt atacttccgg tatgtcccac tgccaaaaaa ctgtcattac
     2581 cccatacaac actgtaaagg catttgtcat agtcaatacc ggtgtcattg cttaacttat
     2641 ttgtgtctat ctgctgccat gcctttccgt catcagaaac aaataccgaa atatgatctc
     2701 cgacaactgc ataactgctg ccgttccagg aaacatccat aaaggccaga gtagaagagg
     2761 ggtcaaacat tatctcttcc catgttatgc cgtcatcgga agacagaatg agcattccac
     2821 cgcccaccgc catatattta ccgttaaacc attttacact cataagagat tccattgtcg
     2881 ccgtccttat ccgcttccac tccgtaccgt tcgaagatac caatacagtt cccgaattac
     2941 cgacagcaac aagctgttca ccgttaaatg cgaccgattg aagatctaaa tttgtgtccg
     3001 attctacctg aacccagttt aatccattct ttgaagttaa tattgttcct tcgtcaccga
     3061 cggcaataaa ccggttcatt ccccaaacaa catcattaag ggtgtggttt gtatcagact
     3121 cgatttttga ccaattaatc ccgtccctcg acataaaaat aactccttcg aacccgactg
     3181 caacataaag gtccgaaccg ttaaaggcta taccgttgag ttccggttca tcggaggttt
     3241 gtgcaaatac aaaggaacaa ttcataacca taattgttag tacaaagaaa cataacagct
     3301 ttttcataga tcaactctcc ctaaagtatt cctcataatt ctattttaac atactttaga
     3361 ggcaaattat aaaaaaagtc gaaaaatata caatgaatta tatatattac aatttacaga
     3421 tttttgaacg tacaatagag tttatctatt ttgtcggaat tatataataa ggttcgtctc
     3481 cctttaaatc cacatagagg tttccttcag tatccagttg tgctaaggct attttgccga
     3541 tatcggttat gtttttcctg gacaattggt gatataacca ggctttggag agcttcacag
     3601 aatgcaaggc atcttctata attacaccgt ctaaaatgag gtttgtcggc aagccggcat
     3661 actgagttga aagccctaga tctttaggtg ttatgggctg caattgtgat ttttttgtac
     3721 gcttattttc ccactaggct caaacatggc aaattcaacg tcggcaacat taaatacccc
     3781 ttgggttctg agctcagaaa aaagctgttc caaaggcatg cggatcttat taagattgct
     3841 atccaatatt tttccatttt caataacaat agttttcttt cctataacga tttttctcat
     3901 ccacgcactg tgcattgaag aaacagccaa taataaagct aaaacggtcc agacgatcaa
     3961 tccggctaaa gtagcagtga aattatcatc tgtctggacc gacagattgg aagcaataga
     4021 acctattgta attccgacaa catagtcaaa atgggtaagc tgtgatacct gttgcttacc
     4081 taacaaacga acaagaagca acagaacgaa aaatgatata accgaacgaa ggactacttc
     4141 cgaaataatg gacataaaaa cctccaaaca agagaaataa tcatttattc aacacatatt
     4201 atttcccgaa aaaaatgaat tatcaaatcc gaaaatcgaa ttgtaaagcc tgtatccgcc
     4261 gctcaaatta tacacatcaa atccgttctg cgaaagaatc ctggctgcaa tatatccccg
     4321 gaggcccacc tgacaggtta catatacagg cttggtctta tcgatttccg tcaatctgct
     4381 tcttagagaa tccaagggta tattaacaaa tcctttaata tgacctctat tatattcatt
     4441 gactgtacgg atatcgagaa gtgtaacact tccgtcacgg ggaaggttat ttacatcatg
     4501 ccaatgaaat atctttacct tgtttgtaag tatgttttcg ataacatagc ctgccatatt
     4561 gacaggatcc ttggcagaac cgtatggcgg agcatagcac agttcaagcc gtgtcaagtc
     4621 aaaagcagtc attcctgcac gtatagccgt tgcaaataca tcgcatctct tatccgagcc
     4681 ttcgtatcct actatctgca ctcccaaaat cttgccggtt cccttctcat agatagtttt
     4741 tatggacata cctttagctc cgggataata gcttgcgtga ttggctgaat aagtatagga
     4801 tttatcataa ttaagaccca aatgctttgc agttttctca ttaattcctg tagaagcaac
     4861 ggttaagtca aacactttta ttatggatga accctgtgta ccggtgtata tgctgtcgat
     4921 accgcagaca ttgtcagcgg caatccttcc ctgtttgttc gcaggacctg ccaaaggaac
     4981 aaagcccttg attcccgtta caaaatcggt tatttccacc gcatcgccca cagcataaat
     5041 atctgtttcg ctggtccgca tatgttcatc aacaataatt gaaccccgct cattaacggc
     5101 tatacctgcc tcctttgcaa tttgggtttc gggtttgaca ccaacggcca taatcaccat
     5161 atcggcattg atattaccac tgtccaaggc aatatcgaga gaatctcctt tatcggcaat
     5221 ggagcgaaca gagttgccaa ggaaaagctt cacacccttg ctcacaatgt gattatgcac
     5281 atcacacgcc aaatcataat caataggtgc aataagctga ttcaccattt ccacaagggt
     5341 tacaccaatt cccctttcat gaagattctc agccatctca acaccgataa accctccacc
     5401 gacaaccact gcacttttag gtttgtaagc ctcaatatat tcatttattt tataagtatc
     5461 gggaatattt ctcagcacaa aaaccttatt tgagtctatt ccttcaattt taggcttcag
     5521 cggctccgca cccatcgaga gaataagctt gtcat
//
LOCUS       DAOOJC010000115         5548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_11788_length_5548_cov_3.960495, whole genome shotgun sequence.
ACCESSION   DAOOJC010000115 DAOOJC010000000
VERSION     DAOOJC010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11788_length_5548_cov_3.960495"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLA01_04490"
     CDS             complement(<1..232)
                     /locus_tag="PLA01_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucanase"
                     /protein_id="HPD00591.1"
                     /translation="MYKRVISLLTASLLTVSLLFVLPAIISPSSGVNAANVVTTPFEA
                     VFSDFDSSLWEKASWANGSVFNCVWKPSQVTFS"
     gene            553..1614
                     /locus_tag="PLA01_04495"
     CDS             553..1614
                     /locus_tag="PLA01_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPD00592.1"
                     /translation="MNSKDIAKIVGVSRSTVSRVINNYPDIPPATREKVLKAIKEYNY
                     YPNASARRLAGMKSSTLGLFIIDIKDNEKPHHVIEKNEDLLYGNSYFSPFINAFIDQS
                     NKAQYHVLVSTVYSSDELWKIQSAFCEKRIDGAVIIGSSKADYKKIVEIMDKNFIMVA
                     VDLVVEEENMDAVLSVNVNNYGGVSDAVEYFIELGHRDIGIITGDLSKFSGKERFDSF
                     KHVLSKHCLPLNNDFIAYGDFTEYSGYEGMKKILAYEKKPTALFVSNDTMAIGAYRAI
                     KERGLKIPEDISVIGFDNSYISQYMSPSLTSVNVSLPEMAKSSIDLLLESINSKKIKS
                     VHKIVNVEIIKRNSCKKIE"
     gene            1740..4244
                     /locus_tag="PLA01_04500"
     CDS             1740..4244
                     /locus_tag="PLA01_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HPD00593.1"
                     /translation="MKRFAKLICVLTLLAMALGMMQLALAGGSDNESLQQGQPKSGPK
                     SVSQSIEKRLENAIALYIGSSQALVNNEEKQVDSTNAKVMPYIKNDRTMVPVRFISEN
                     LGAQVKWDAQSSKVTVSLGDRVVVLTVGSKTMKVGDKSVDLDVAPELNEDRTYLPLRG
                     LVEGLGKKVFYDRGLIVIGDREDVFDVSGEKALIDEVISRVNNLPVVGTYENLEKMFE
                     KTVANYNKYNGPMPVWNTVQSMDGAESKGSAELAAQPASADEGRSAGGSDDYSTTNVQ
                     VQGVDEADVVKTDGEYIYQVNSNRIVVIKAYNTVTADAYSSPKMKIMSTLTFSDDNFT
                     PQELYIDGDYMVAIGMYYEQRPYIQETKSEEKMSILPYYPAKSTAKAIVFDIKNRADI
                     KKLREVEVEGHYISSRKIDNVLYLITNKDSGAYYENDKLKIQTPTFRDTAVSKDYAEI
                     GYPDIRYFPDSEATNFLNIAAFDITGNEEANIQTYLGAGQNIYASAENLYVAVTNYNY
                     AMLRNGLAADIAPRVNDITTTVYKFSLNGEKVTYLSKGDVPGTILNQYSMDENGGYFR
                     IATTMGQVWGTGQNISKNNVYILGDAMNIVGSIEDMAPGEQIYSVRFMGDRGYVVTFR
                     TVDPLFVIDLKEPSNPKILGSLKIPGYSDYLHPYDENHIIGFGKDTVAIKDNAYYLGM
                     KIALFDVTDVENPIQKFSETIGDRGTDSELLRNPKALLFSKNKNLLAFPVTVMEVRNK
                     NQNIDFPDYGEFAFQGAYVYNIDTENGFVLKGRITHMSEEEYRKAGNYYYNPNKYVER
                     ILYIGDTLYTLSPGMVKAHGLEDMEERDSLEIPENR"
     gene            4600..4782
                     /locus_tag="PLA01_04505"
     CDS             4600..4782
                     /locus_tag="PLA01_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00594.1"
                     /translation="MKVKDRNRLYRVWHTDKKTCSKFDGKEIEEVFATSVKEAKEKVL
                     QKYPAHRVTSAWLIEK"
     gene            4895..>5548
                     /locus_tag="PLA01_04510"
     CDS             4895..>5548
                     /locus_tag="PLA01_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPD00595.1"
                     /translation="MKIPIYDAVKNYSSSCPAAFHMPGHKLGKGLPSAFLESIYLLDL
                     TEIPGLDNLHYPKGVIEEAQALAAQAFGADRTFFLVNGSTCGIQAAIMTLCRPGDKII
                     IARDCHRSAIAGMMLSGATPVYIKPEFNGFFGIPSVVSVNEIEKALKENPDAVGVYIT
                     RPNYYGVCSDIKAIVELVHSYNKPVIVDEAHGAHLCFSSMLPPSSLEYGADICVQSAH
                     "
BASE COUNT         1831 a          938 c         1218 g         1561 t
ORIGIN      
        1 tggaaaatgt tacttgagaa ggcttccaaa cacaattaaa tactgaaccg ttcgcccaac
       61 ttgctttctc ccatagactg gagtcaaaat cggaaaatac tgcttcgaat ggtgtggtta
      121 ctacattagc tgcatttact ccagaagaag gagagataat tgccggcaga acaaacagta
      181 gcgaaaccgt taacaaggaa gccgttaata atgaaataac cctcttatac ataaatacct
      241 cccaataaaa aaaatataat ataattttac cgcagtgagg aattagaacc actgcgaaag
      301 taaaactcag taacacaaac aactaacacg tgttaattca acttaattat atcctatcgg
      361 tttttctttg tcaaccaaac ccaaacctag attaaaaaga atatcattct ataataatgt
      421 tacttataga ccttgagatt atttattgct aaatacataa acagtaaatt ctgcttgatt
      481 aattacaata tcaatgctat cctttacttg taagactttt ttatattttt tcggcattaa
      541 ggtggtttga tcatgaatag caaagatatt gcaaaaatcg ttggggtctc aagaagtact
      601 gtttcacgag ttataaataa ctatcctgat attccaccgg ctacacggga aaaggtatta
      661 aaggcgataa aagaatataa ctattatccc aatgcatcag ccagaaggct tgcgggaatg
      721 aaaagttcta cattgggact ttttattatt gatataaaag ataatgaaaa accccatcat
      781 gtaatagaaa aaaatgagga tcttttatat ggcaattcat acttttcacc ctttataaat
      841 gcttttattg accagtcaaa caaagcccag tatcatgttt tggtatcaac tgtctacagt
      901 tcggatgaat tgtggaagat tcaaagtgca ttttgcgaga aaagaattga cggtgcagta
      961 attataggaa gcagtaaagc tgattataaa aaaatagttg aaataatgga taaaaatttt
     1021 ataatggttg cagttgattt ggttgtggaa gaggaaaata tggatgcggt tttgtctgta
     1081 aatgtcaata actatggcgg tgtgtctgat gccgtagaat attttattga actagggcat
     1141 agggatatag gcattattac aggtgattta agcaagtttt ccggcaaaga aaggtttgat
     1201 agcttcaaac atgtactatc caagcattgc ctcccgctaa acaatgattt tattgcctat
     1261 ggtgatttta ctgaatacag cggatatgaa ggcatgaaaa aaatactggc atatgagaaa
     1321 aagccgacag ccttatttgt atccaacgat actatggcta tcggagccta tagggccata
     1381 aaggaacggg gactgaaaat tccggaggac atttccgtta taggattcga taattcatat
     1441 atatcccaat acatgtctcc ttctcttact tctgtcaatg tctcattgcc ggaaatggct
     1501 aaatcttcta ttgatttact ccttgaatct attaatagca aaaagataaa atctgtacac
     1561 aaaattgtga acgttgagat aattaaacgc aattcgtgca aaaagattga atgaatgtta
     1621 ttacaggata gagccgctgc aaatttttaa tattcacaag atatttatgg aactttttat
     1681 aacaaaatgc attctaaact aatataaaaa taaataatta ttttgaaagg agcattaaaa
     1741 tgaaaagatt cgcaaagctg atctgtgttt tgacacttct ggcaatggct ttaggaatga
     1801 tgcagctggc acttgccggt ggcagcgata atgagagcct gcagcagggt caacccaaga
     1861 gtgggcctaa gagtgtatcc caaagtatag agaagaggct tgagaatgct attgctttgt
     1921 atataggaag ttctcaggca cttgtaaata atgaggaaaa acaggtggat tctacaaatg
     1981 caaaagtgat gccctacata aaaaatgaca gaaccatggt acctgtgagg tttatatccg
     2041 aaaacctcgg tgcacaggtt aaatgggatg ctcaaagctc taaagtcacc gtttcattgg
     2101 gtgacagggt ggtggtgctt accgttggaa gcaaaacaat gaaggtaggg gacaaaagtg
     2161 ttgatcttga tgtagcaccc gaattgaatg aagacaggac atatctgccc ttgagaggtc
     2221 ttgtggaagg cttgggcaaa aaggtttttt acgacagggg acttatagtt atcggtgaca
     2281 gggaggatgt atttgacgta tccggggaaa aggctttgat tgacgaagtt atttcaaggg
     2341 taaacaacct tcctgtagtg ggaacttatg aaaaccttga aaagatgttt gaaaaaactg
     2401 tggcaaatta taataagtat aatggaccaa tgccggtgtg gaacactgta caaagcatgg
     2461 atggagcaga atctaaaggc tcagccgaac tcgctgcaca acccgcttcg gctgacgagg
     2521 gcagaagtgc aggcggctcc gatgactatt caactacaaa cgtacaggtt cagggagtgg
     2581 atgaagctga tgttgttaaa accgacggag aatatatata ccaggtaaac agtaatagaa
     2641 tagtagttat aaaggcgtac aataccgtaa ccgcagatgc ttattcatcc ccgaaaatga
     2701 aaataatgag caccttgacc ttttccgatg ataactttac accgcaggag ctctatatag
     2761 acggggatta tatggttgcc atcgggatgt attatgagca aaggccttac atccaggaaa
     2821 caaaatctga ggagaagatg agtatacttc cgtactatcc cgcaaaaagt actgcaaaag
     2881 caatagtatt tgatattaaa aaccgggctg atatcaaaaa gctaagagag gtagaggtgg
     2941 agggacatta tatttcttca cgaaagatag ataatgtgct ttatctcatt accaataaag
     3001 attccggtgc ctattatgaa aacgacaaac tcaagataca gaccccaacc tttagagata
     3061 ctgctgtttc gaaggactat gcagaaatcg gctaccccga tataagatat ttcccggatt
     3121 ctgaggcaac aaactttttg aacatagctg cttttgatat aacgggcaat gaagaagcca
     3181 atattcagac ctatcttggt gccggtcaga atatttatgc atcggctgag aacttgtatg
     3241 ttgctgtaac caattataat tacgccatgc tccgcaatgg attggcagct gatattgcac
     3301 caagggtgaa tgacataacc acaacggtat ataagttttc gctcaatgga gaaaaggtta
     3361 cataccttag caagggagat gtacctggaa ccatattgaa ccagtattcg atggatgaaa
     3421 acggcggata tttccgtatt gccacaacaa tggggcaggt atggggaacg ggacaaaata
     3481 tttccaaaaa caatgtttat atactgggcg atgccatgaa tattgtggga agcatagaag
     3541 atatggcacc gggcgagcag atttattctg tgaggtttat gggagaccgg ggctatgtgg
     3601 tcacattcag gacggttgac ccgttgtttg ttatagacct gaaggaacct tcaaacccga
     3661 aaatactcgg aagcttgaaa ataccgggct atagcgacta tttgcatccc tatgacgaga
     3721 atcatatcat tggtttcggt aaggatacgg tggcaattaa agacaatgcc tactacctgg
     3781 gcatgaaaat tgcacttttt gatgtgaccg atgtggaaaa tcccatacaa aagttctctg
     3841 aaacaatagg agaccggggc actgattcgg agcttctgag aaatcccaag gcacttttgt
     3901 tctcaaagaa caaaaatctg ctggcctttc ctgtgacggt catggaggtt cggaacaaga
     3961 accaaaacat tgatttcccc gattatggag aatttgcctt ccagggtgct tatgtctaca
     4021 atatcgatac tgaaaacggt tttgtattaa agggcaggat tacccatatg tccgaagaag
     4081 aatacagaaa agcaggaaat tactattaca atccaaataa gtatgttgaa aggattttat
     4141 atatcggtga taccctctat accttgtctc cgggcatggt taaggctcat gggcttgagg
     4201 acatggaaga gagagacagt ttggagattc cggaaaaccg ttagtcggta tgcaataaaa
     4261 cggcaatctt aacggtccga ctagtggttt ttatgtactc actgttttta ggtgcgatta
     4321 ttgataggag agagatattt taaaaaactt tagtcattta gaatatttct cttctttttt
     4381 tgttctgttt attttgataa aatatagaaa ctgataaata ttttacttta aatagtcata
     4441 ataaatgata taataattat gtccttgcat taaggctgat aatctcagtg gggaaagaga
     4501 gggtattttt ctttgctggc aaaaagctga aatgcgtttt gacatttatg aaggattgca
     4561 taaagtcaaa agtttattta tgtaaggcag gggaacaaaa tgaaagtgaa agacaggaat
     4621 aggttgtaca gggtctggca tacggacaag aagacctgtt caaagtttga tggcaaggaa
     4681 attgaggaag tatttgctac gagtgtaaaa gaagcaaagg agaaggtttt gcaaaagtat
     4741 cctgctcaca gggtaacatc ggcatggctt attgagaaat aatgcttctt aaatacagaa
     4801 atcaggcaaa cggatttcgc tacattgcta atcgaaaaat ggtgtaacga aattactgca
     4861 gttacttctt atggggtgag ttgttatcaa aaacttgaag atccctatat atgatgccgt
     4921 aaaaaattat tcctcttcat gcccggcagc ttttcatatg ccagggcata agcttgggaa
     4981 agggttgcca tcggcatttt tagagagtat atatttgcta gatttaacag aaatacccgg
     5041 actcgacaat cttcattatc caaagggcgt aatagaagaa gctcaggctc ttgcagcaca
     5101 agcctttggt gctgacagaa ccttttttct tgtaaacggt tcaacctgcg gaatacaggc
     5161 ggcaataatg actctttgca gaccgggaga caagattatt attgcaaggg attgccacag
     5221 gtctgctatt gcagggatga tgttatcggg agcaactccg gtttatatta agccggagtt
     5281 taacggtttt tttggcatac cttcagttgt ttcggttaat gaaattgaga aagcactaaa
     5341 ggaaaatccc gatgctgtcg gagtttacat aacaagaccc aactattatg gggtatgctc
     5401 ggatattaag gcaatagttg aactggttca ttcctataac aagccggtta tagtggatga
     5461 ggcccatggt gcccatttgt gtttttcaag tatgcttccc ccttcgtcac tggaatatgg
     5521 agcggatatt tgtgttcaga gtgcacac
//
LOCUS       DAOOJC010000116         5535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_11826_length_5535_cov_3.733029, whole genome shotgun sequence.
ACCESSION   DAOOJC010000116 DAOOJC010000000
VERSION     DAOOJC010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11826_length_5535_cov_3.733029"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PLA01_04515"
     CDS             <1..978
                     /locus_tag="PLA01_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00596.1"
                     /translation="VVTDTFIEMEPRGNTWKGNVSLSGGIFDSFSKEGVPGLEMNISI
                     GNLGVFETAVSDEYGNFTEEMILEIPESLSPDKDFFFVSGYNLAYNVDFKGTDIYYPS
                     RVEDSFFLWNVHWLRIILSLVIFASAIIIICILIKKKLLFSKSKVQTVLETGVGGIAN
                     DSYTGYDAIMENLPTGMCHIHFPQIAEDFPSVWGVEDELLVSFFDSEGNNGEFWCSFH
                     KKGDYNIRLCKDKGIPVFKDIRIVVYREEIITIGKDYLKGIYGTLPDISSVMTLREIR
                     DKIKSRIPEEKQGMLEGAFSILEKALYSNKDIVRNDYERFYVFINKLQS"
     gene            1006..2181
                     /locus_tag="PLA01_04520"
     CDS             1006..2181
                     /locus_tag="PLA01_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00597.1"
                     /translation="MQARGKVAADATFNKLMVYPIVLLLYIGITVFLFFIPLGPGYSI
                     RQSFYFLIYSIEALLLGIVLKHIVSYYLKGKWAEVISDSCFIMGIGFAVFLFFFYLLP
                     YGSVRAASIGVLILAASVEVFRISELFKGRVFEGSLIKAVSDLIAGITIYLMFGALCP
                     EGYLDIGLKISIGKIFFFSFFILAVFQMISLLDFVEDRRISRITLWFRTNHKVKFFAV
                     FLVLYLLIDLRRDIMGDAVLVSWIFIFIVLVIVFIVLTVKLSATVNRSPEQKLNKHLQ
                     KISFEKIKDITNISKCIDDFVRHGSKGGIIACLYYMAYKTGIPISLASNIIAPVVECK
                     DLEMPSLATRKNYLVIEERNRQNRLTVIEKVIYNLELYGRGNFYGYRADSNTMPKHN"
     gene            2138..3127
                     /locus_tag="PLA01_04525"
     CDS             2138..3127
                     /locus_tag="PLA01_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPD00598.1"
                     /translation="MDTEQIQTQCQNIIKEVGKVFVTDDDTILNKILCGFLAGGHILF
                     EDNPGLGKTLLVKVFARVTGCEWKRIQFTPDLMPSDILGTKIWRSNSSSFELEKGPIF
                     TNFLLADEINRAMPKTQSALLEAMEERQVSIEGTTYILKPPFTVMATQNPIEMEGTYP
                     LPEAQMDRFIMKLSLGYLESTQKECEVLKRRLEWGKDDPTNDVRNVVDMNQVLALQAE
                     VEKVFVDENILMYITEIVRSTRTNTNVRLGASPRGALALLKLARAHALICGRDYVIPD
                     DVKFLAVDALAHRVILHIEHIMEGRVPEAVVRSIVSKIDVPKSYTREKVGGLV"
     gene            3128..4453
                     /locus_tag="PLA01_04530"
     CDS             3128..4453
                     /locus_tag="PLA01_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPD00599.1"
                     /translation="MPKYISEMSIVFLLMLCGGFLLANNILIIVSVIPLCLLAFGYYL
                     KTSNDVIVKKTISKSRVAVDEGLEVNLKIWINSGIGPIEICDLVPAHFELVSGSNFCV
                     LWKGFEPLEHCLSYTIKCTTSGTYMLKTTLWKSRHVVCNYNIDGMYVNDLSVEVTPRL
                     LQLKKVRGMTAVSSVPLPEGALASMGMTTQEFKEIRQYYFGDPFKSINWKATSRNLLR
                     GNIWPVVNEFEKEGKKTVWIFHDTSRIMAFGSNIKNVKEYSIEAVYSLSEFYLGHNCS
                     VAFHTYGGNNVFIKQGSGKQQLYKILRELMKIKYSGPAVLSPRVHIKNLEESVFSCRE
                     FFVGLRPLFVIVTRFCTNNFNEILKGINTMSKYTMLRKGYLPSIMVVNIMGYGLMAEN
                     QYEILASNVLEAMNRVISNDIRKNCIWIDWDPTKESLTGALLKQVVAAK"
     gene            4450..5070
                     /locus_tag="PLA01_04535"
     CDS             4450..5070
                     /locus_tag="PLA01_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00600.1"
                     /translation="MKYWTLSLRIAFALVAYVMSFILLFYTPVYQEFVYMFSITKVFI
                     ITNIIALVIIGIGYKRVLIVESIVCTVFCAPSVLYHSKLVRQIFDIQPAQYLSIQITA
                     FMFLIIAMFLMAAARLERLEKGSEEMLSGGACEADVILIWSNSLKIYSAFFALVLLLC
                     IGLISLGFFAPHITASKQLLIIMMVTGITLIVGSVFFLYGKWVKKH"
     gene            5348..>5535
                     /locus_tag="PLA01_04540"
     CDS             5348..>5535
                     /locus_tag="PLA01_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00601.1"
                     /translation="MRVKTCLICYVLIVCMLISSISVAASGLNAGTDANQNSPVKSSY
                     SQTFPDIEGHWCKEYIEK"
BASE COUNT         1685 a          821 c         1272 g         1757 t
ORIGIN      
        1 gtggttacag atacttttat agaaatggaa ccccgaggta atacatggaa gggaaatgtc
       61 agcttgtcgg gaggaatttt tgacagcttt tcaaaagagg gcgttcccgg actcgaaatg
      121 aatatatcca ttggtaatct gggggtcttt gagacagcgg tttctgatga gtatggaaat
      181 tttactgaag aaatgatttt agagattccc gaaagcttgt cacccgataa ggatttcttt
      241 tttgtcagcg ggtataattt ggcatacaat gttgatttta aaggtacgga catttattat
      301 ccctccaggg tggaagacag tttttttttg tggaatgtgc actggttaag gattatattg
      361 tccctggtga tttttgcttc cgcgattatt ataatatgca tattgattaa aaagaagtta
      421 ttattctcta aaagtaaagt tcagacggtg ctggaaaccg gtgttggggg catcgccaat
      481 gattcgtata cgggttatga cgctattatg gaaaacctgc cgacaggcat gtgccatatt
      541 cattttccac aaattgctga ggatttcccg tctgtatggg gtgtggaaga cgagttatta
      601 gtgagctttt ttgatagtga agggaataat ggcgagtttt ggtgcagctt tcataaaaag
      661 ggagactata atattaggct ttgtaaggat aaaggcatac cggtatttaa agatataaga
      721 atagtagttt accgtgaaga aataattaca atcggaaagg actacctaaa aggcatttat
      781 ggaactcttc cggatattag ttcggtgatg acattaagag agattcgtga taagataaag
      841 agccgaatac ccgaggaaaa gcaagggatg cttgaggggg ctttttcgat attggagaag
      901 gctttgtaca gtaataaaga tattgtaaga aatgattatg aaaggtttta tgtatttatt
      961 aacaagttac agagttaacg aaatacaaat ttctggagga ataagatgca agcacgaggg
     1021 aaagtggctg cggatgcgac atttaataag ttaatggttt atcctatagt gcttttattg
     1081 tatattggaa taacagtatt tttatttttt atcccattag gtccggggta tagcatccgg
     1141 caatcttttt attttttgat atactccatt gaggcactcc ttttgggaat agttttaaaa
     1201 catatagtaa gctattactt gaagggtaaa tgggccgaag ttataagcga ttcctgcttc
     1261 ataatgggaa ttggctttgc tgtatttttg tttttcttct atttattgcc ctatggttca
     1321 gtaagggcgg cgagtattgg ggtactgata ttggcggcat cagtagaagt gttcagaatt
     1381 tcagaattat tcaaaggcag ggtgtttgag ggaagcttga taaaggctgt ttccgatctg
     1441 attgccggaa tcacaattta cttgatgttt ggtgcccttt gccccgaagg atatttggat
     1501 attggtctta aaatatcgat aggtaagatt tttttctttt ctttttttat tcttgctgtt
     1561 tttcaaatga tatcgttatt agactttgtg gaagaccgga gaatcagcag gattacttta
     1621 tggtttagaa ccaatcataa agtgaaattc tttgcggttt tcttggtttt atatttatta
     1681 attgatttaa ggagggatat tatgggagat gccgttttag tgtcgtggat attcattttt
     1741 attgtattgg tgattgtatt tattgttctg acggtcaagt tatcggcaac tgtaaataga
     1801 agtccggagc aaaagcttaa taagcatctt cagaaaattt ctttcgaaaa gataaaggat
     1861 ataaccaaca tttcgaaatg tattgatgac tttgtccgcc atggcagtaa aggaggaata
     1921 attgcctgcc tttactatat ggcatacaaa acggggattc caattagtct ggcaagtaac
     1981 attatagctc ctgtagtgga gtgtaaggat ttggaaatgc cttctctggc aacaaggaag
     2041 aactaccttg ttattgagga gagaaatcgt cagaacaggt tgacagttat agagaaagtt
     2101 atatataatt tagaattata tggaaggggt aatttctatg gatacagagc agattcaaac
     2161 acaatgccaa aacataatta aagaagtagg taaggtattt gttaccgatg atgatacaat
     2221 tcttaacaaa atattatgcg gatttttagc cgggggacac atactttttg aggacaatcc
     2281 cggccttgga aagaccttgc ttgtaaaagt atttgcgagg gttacaggct gcgaatggaa
     2341 acggattcag tttactccgg atcttatgcc ttcggatatt ttaggtacaa aaatatggag
     2401 aagtaattca tcttcctttg aattggaaaa ggggcctata ttcactaatt ttcttttggc
     2461 agatgaaatc aatagggcta tgcccaagac ccagtctgca ttacttgagg cgatggagga
     2521 aagacaggtc tcaattgagg gaactaccta tatattaaaa ccgccgttta ctgttatggc
     2581 tacgcaaaac cctatagaga tggaaggaac atatcctctt ccggaggcac agatggacag
     2641 gtttattatg aaattatcct tagggtattt agaaagcact caaaaggagt gcgaggtctt
     2701 aaaaaggaga cttgagtggg gaaaagacga tcccaccaat gatgtacgga atgtggtaga
     2761 tatgaatcag gttttggctc ttcaggcaga agttgaaaaa gtttttgtgg atgaaaatat
     2821 cctaatgtat attacggaaa ttgtacgcag caccagaaca aataccaatg tgaggttagg
     2881 ggcaagtcca aggggagcac tggcattatt gaagcttgca cgggcacatg cccttatttg
     2941 cggcagagat tacgtaatac ctgatgatgt caagttttta gctgttgacg ctctggctca
     3001 tcgtgtcata cttcatatag aacacataat ggaaggcagg gttcctgaag cagtggtaag
     3061 gagtatagta agcaaaatag atgttcccaa aagctatacc agggaaaagg taggagggct
     3121 tgtataaatg ccgaaatata tctccgaaat gagcattgta tttctattga tgctttgcgg
     3181 aggctttctt ttagccaaca acatactgat aattgtttct gttatccctt tatgcttgct
     3241 ggcgtttggt tattacctca agacttccaa tgatgtaatc gtaaaaaaga caatatcaaa
     3301 aagcagggta gctgttgacg aagggcttga ggtaaattta aaaatatgga taaattcagg
     3361 aattggaccc atagagatat gtgaccttgt tcctgctcac tttgagcttg tgagcggcag
     3421 taatttttgc gtattatgga agggttttga gcctttagag cactgcttga gctatactat
     3481 aaaatgtacc acatcgggaa cctatatgtt aaagacaact ttatggaaat cccggcatgt
     3541 tgtatgcaat tacaatatag atggaatgta tgtaaatgat ttatctgttg aggttactcc
     3601 aagactttta caactgaaaa aggtgagagg aatgactgcc gtaagcagcg taccgttgcc
     3661 ggagggggca ctggcttcca tgggaatgac aacgcaggag ttcaaagaaa tccggcaata
     3721 ctatttcgga gatcccttta aatcaataaa ctggaaggct acttcgagaa acctgttaag
     3781 aggcaatata tggcctgtag taaacgagtt tgagaaggaa gggaaaaaaa ccgtatggat
     3841 ttttcacgat acatcgagga taatggcgtt cgggtcgaac ataaaaaatg ttaaagagta
     3901 ttcaattgaa gccgtataca gcctgagtga gttttatctg ggacacaact gcagcgtagc
     3961 ctttcatact tatggcggta acaatgtttt tattaaacag ggatcgggaa aacagcagct
     4021 gtataagata cttagggagc tcatgaaaat caagtactcc gggcctgcag ttttaagtcc
     4081 gcgggtgcat ataaaaaatc ttgaggaaag tgtattttca tgtagggagt tttttgtcgg
     4141 tttaagacct ttgtttgtaa ttgtgacaag gttctgtaca aataatttta atgaaattct
     4201 taagggcata aatacaatgt caaaatacac tatgctccgg aaaggatatc ttccgagtat
     4261 tatggtagtg aatataatgg gatatggcct aatggctgaa aaccaatatg aaatactggc
     4321 atcaaatgtg cttgaggcta tgaatcgagt gatttcaaat gacatcagga agaattgtat
     4381 atggattgat tgggatccga caaaggaaag ccttacggga gcgttattaa aacaggtggt
     4441 ggcggcaaag tgaaatactg gactctgtct ctaaggattg catttgccct ggtggcttat
     4501 gttatgtcct ttatcctttt attctataca ccggtttatc aagaattcgt gtatatgttt
     4561 agcattacaa aagtatttat tattacaaat ataattgctc ttgttataat cggcataggt
     4621 tacaagaggg tattaatagt tgagagtatt gtgtgtacgg tgttttgtgc accttctgta
     4681 ctataccatt caaagctggt cagacagata tttgatatac agcctgccca gtatttgtct
     4741 atacaaatca ctgcttttat gtttttgata attgctatgt ttttaatggc agcagcaagg
     4801 cttgaaagac ttgaaaaagg atcggaggaa atgttatccg gcggtgcttg cgaggcggat
     4861 gtaattttaa tttggagcaa tagcctcaag atttattcag ccttttttgc attggtatta
     4921 ttattatgca tcggtcttat ttcccttggt ttctttgctc cgcatataac agcctcaaaa
     4981 cagctgctaa taataatgat ggtaacgggt attacactga ttgttggttc ggtgttcttc
     5041 ttatacggga aatgggtaaa aaagcattga atttttccac ggtctgttga caggttctgt
     5101 aacccgcttt acaatttttt catatgtact tatataaata gtgtgcacgt gttgcaaagg
     5161 acacgacact aaatttataa aaataattta attgatataa ttcagtcgtt gtaatataat
     5221 taataaaaat gtccatttgt aaagtattag taaatcagct gtataacagt agtgatttat
     5281 taatatgtag gttctattct atattattaa ttgattgtct ggatattaat gttttggggg
     5341 gacaataatg agagtaaaaa catgtctgat ttgttatgta ttgattgtct gcatgcttat
     5401 tagcagcatt tctgttgctg cttccggact taatgccggg accgatgcta atcaaaattc
     5461 accggtcaaa agcagctatt cacaaacctt tcccgatata gagggacact ggtgcaaaga
     5521 atacatagaa aagtt
//
LOCUS       DAOOJC010000117         5532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_11831_length_5532_cov_4.387986, whole genome shotgun sequence.
ACCESSION   DAOOJC010000117 DAOOJC010000000
VERSION     DAOOJC010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11831_length_5532_cov_4.387986"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..539)
                     /locus_tag="PLA01_04545"
     CDS             complement(126..539)
                     /locus_tag="PLA01_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HPD00602.1"
                     /translation="MYCRNCGNEMDGQAAICVKCGVAKNNGSNFCHNCGQGTNAGAQV
                     CVNCGVNLIAGGEQKSKMAAGLLGIFLGGFGIHRFYLGYTVIGIIQIAVTIGVAIITC
                     GVGSFVGPLWGLVEGILILCGSFITTDAKGNPLKD"
     gene            complement(645..2141)
                     /gene="hemZ"
                     /locus_tag="PLA01_04550"
     CDS             complement(645..2141)
                     /gene="hemZ"
                     /locus_tag="PLA01_04550"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HPD00603.1"
                     /translation="MRIYIRLEEHDFKYEIEDVIRLFFGDGEISIEYRDPGEDNPGFI
                     LYSGLESSLNIDGQYHIKTILKADGNIIFSENEIFTISSDDEYEVKKIKKREIKRQVY
                     RALSRYMAKKMPWGVLTGIRPAKIVHELMTKGFNREQIYSKLTDYYYVSGKKAELLYD
                     VAQKEKHILDSTGPDKVSIYIGIPFCPTRCLYCSFTSNTIKKYAHFINNYIEALKKEI
                     YAVGEILDSKGFTVQSIYLGGGTPTSIDASYLKELLTHIEKSFYMKETKEFTLEAGRP
                     DSISSEKLEIIKKSKVNRISINPQSMNDEVLRNIGRLHSAKDIVDAFKLARSLGFDNI
                     NMDLIAGLPGEKLEGFKNTLERVGELGPESITVHTMAIKRASRLNEDKDSYSLISGNE
                     VAKMVDAAYDFITRNGLEPYYLYRQKNMLGNLENIGYSKPGYECMYNIQIMEEKQTII
                     ALGAGAVTKVVFPENNRIERTFNVKSVEEYVDRIEEMIERKRSLIDYC"
     gene            complement(2181..2783)
                     /locus_tag="PLA01_04555"
     CDS             complement(2181..2783)
                     /locus_tag="PLA01_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD00604.1"
                     /translation="MILECIPYGLFGSNCYIVGDKGEAVVIDPGCDPNEIIRVAEANS
                     LSIKYIIITHGHIDHLVSVEELRKKTGAKVLIHKDDADKLTDPSLNDALGMGLGITGQ
                     KADRLLQDGDRLSVGGIDFEIIHTPGHSAGGICIKAEDKVFTGDTLFKMGIGRTDFID
                     GDLETLFNSIKTKLFTLVDSTVVYPGHGEPTTIGAEKRSF"
     gene            complement(2857..3306)
                     /gene="dtd"
                     /locus_tag="PLA01_04560"
     CDS             complement(2857..3306)
                     /gene="dtd"
                     /locus_tag="PLA01_04560"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPD00605.1"
                     /translation="MRAVVQRVTSSKVTVEGETIGEIQKGLSVLLGVGDDDTDRDIEY
                     LADKIVNLRIFEDENGKMNLSLKDVGGDLLVVSQFTLYGDCRKGKRPSFDKAAGAKKA
                     EEFYERFVKVCRNYDLKVETGKFQAEMMVEIYNNGPVTILLDSKKEF"
     gene            complement(3341..5518)
                     /locus_tag="PLA01_04565"
     CDS             complement(3341..5518)
                     /locus_tag="PLA01_04565"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPD00606.1"
                     /translation="MGDGYNRLIRKIKEYNPNCDFNLIEKAHNISLEAHQGQQRVSGD
                     PFIVHPMEVAIILADLELDSTAIVAGILHDTIEDTTFTYEEIKEEFGEEVASLVDGVT
                     KLGKIPYTSKEELQAENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMTPEKQIEKA
                     KETLEIYAPLAHRLGISKIKWELEDLCFRYLDSKGYYELVNKIAQKRREREAFIAKII
                     ETLKEKTTEIGIEAKIEGRPKHFYSIYRKMIQQNKTLDQIFDLFALRVIVHSVKDCYA
                     VLGLVHEIYKPLPGRFKDYIAMPKPNMYQSLHTTLIGHEGIPFEVQIRTEEMHKVAEV
                     GIAAHWRYKQGGTSDKESDNKLAWLRQLLEWQKEMRDANEFMETLKIDLFTDEVFVFT
                     PKGDVISLPTGSTPIDFAYSIHSAIGNKLSGAKVNNKLVPIEYVLKNGDIIEILTSSN
                     VQGPSRDWLKIVKSSQAKNKINQWFKKEKREENIIRGKEMIERELKKQAVTYNQLFKP
                     EWIEIILRKYTFGTLDDIYAAIGYGALTANKVISRLKEEYRKSVKLDEITEFVSEAVE
                     QKKKTASKSVPENGIIVKGIKNCLVRISRCCNPVPGDDIIGYITRGRGVSVHRKDCIN
                     ISNNIDGDNRLIEVSWYTSNNVAYKADITVMAHDRTALLMEITNVIGESKIPLKAMNA
                     RTTKDQIAIMNLTLEITNTEQLEKIVKKIRRVNGVFEVTRNRQ"
BASE COUNT         1495 a         1331 c          860 g         1846 t
ORIGIN      
        1 gggcctcaaa ccctcatcgt cttttggtca tttcacttcg tgaaatccaa ctaataaaaa
       61 agtccggatt aaaaacccgg acatataaat tcataatgtt gttaaaatac tgaactgttt
      121 aattgttagt ccttcaaagg attacctttg gcatccgttg ttataaagct tccgcaaagt
      181 attagaattc cctctactaa accccagaga ggtcctacaa aggaacctac tccacatgta
      241 ataattgcta caccaatagt gactgcaatt tgaattattc ctatcactgt ataacctaag
      301 taaaatctgt gtatcccaaa tccgccaaga aaaataccca gtagaccagc tgccatctta
      361 gatttttgtt caccgccagc aattaaattt acaccacaat tgacacaaac ttgggcacct
      421 gcatttgtcc cttgaccaca gttgtgacag aaattacttc cattgttctt tgccacacca
      481 cattttacac atatcgcagc ctgaccgtcc atttcattac cacaatttct acaatacata
      541 ttggccctcc ttgtatttag gataattaaa aaaatacatt tctctctctt atataataat
      601 acttttttat tcagatattt gcaatagtaa atacattcca tgatttaaca atagtcaatc
      661 aaactgcgtt ttctttcaat catctcctca atacggtcaa catactcctc aacgctctta
      721 acattaaaag tcctctctat ccggttattc tcaggaaaaa caaccttggt tacagctcct
      781 gcccctaaag ctattatcgt ctgtttttcc tccatgattt gaatattata catacactca
      841 taccccggtt tgctgtaacc tatgttttct agattgccga gcatgttttt ctgccggtat
      901 agataatagg gctctagacc gtttcttgtt ataaaatcat aagcggcatc caccatctta
      961 gcaacctcat ttccggatat caggctatag ctgtccttgt cttcgttaag ccttgatgcc
     1021 cgcttgattg ccattgtatg aaccgtaata ctctcgggac cgagttctcc gactctttcc
     1081 aaagtatttt taaagccctc aagtttctct cccggaaggc ctgctattaa atccatgtta
     1141 atattgtcaa atcctaaact ccttgcaagc ttgaaggcat caacgatgtc ctttgccgag
     1201 tgaagccttc cgatatttct caagacctca tcattcatgg attggggatt tatacttatc
     1261 ctgtttactt tgctcttttt tattatctcc agcttttcgc tgctgatcga gtccggacgg
     1321 ccggcttcca atgtaaactc ctttgtttcc ttcatataaa aagacttctc aatatgggta
     1381 aggagctctt taagataaga agcatcaata gaggtaggcg tacctcctcc cagataaata
     1441 ctctgaaccg taaacccctt tgaatcgaga atttcaccga cagcatatat ttcttttttt
     1501 aaagcctcaa tatagttatt tatgaaatgg gcatatttct ttatggtatt tgatgtgaaa
     1561 gagcagtaca aacatcttgt ggggcaaaaa ggtatcccga tataaatgct gaccttatcc
     1621 ggtcctgtac tgtccagtat gtgcttctcc ttttgagcaa cgtcatataa gagttctgcc
     1681 tttttcccgg atacataata ataatcggtt aattttgagt atatttgctc ccggttaaaa
     1741 cctttggtca ttaattcatg tactattttg gcgggtctta tacccgtcaa aacaccccaa
     1801 ggcatttttt ttgccatgta ccgtgacaaa gccctgtaaa cctgcctctt aatttccctc
     1861 ttttttatct tttttacctc atactcatca tccgacgaaa ttgtaaatat ctcattttca
     1921 ctaaatatta tattgccatc tgctttgagt attgttttta tatggtattg accgtcaata
     1981 tttaacgaac tctccaagcc ggaatacaaa ataaaacccg gattatcctc tccgggatcc
     2041 cgatattcta tgcttatttc tccatctcca aaaaacagcc ttattacatc ctctatctca
     2101 tatttaaaat catgttcttc aagtcttatg taaattctca tctcactccc accagccttg
     2161 cacagcttaa aaccttgtaa tcaaaagctt ctcttttcag ctcctattgt agtaggttca
     2221 ccatgtccgg gatatacaac agtgctgtca acgagtgtaa acagctttgt cttaattgag
     2281 ttgaaaagag tttccaaatc tccatcaata aaatcagttc ttccgatacc cattttaaaa
     2341 agggtatcac cggtaaaaac cttgtcttct gcttttatac aaataccacc tgcagaatgc
     2401 ccgggggtat ggattatttc aaagtcgatt cctccaacgc tcagcctgtc cccgtcttgc
     2461 aataacctat ctgctttctg acctgtaatg cccagtccca tgccaagggc atcgtttaaa
     2521 gaagggtcgg taagtttatc ggcatcatcc ttatgtatta gaacctttgc ccctgtcttt
     2581 ttacgcaatt cttccacgga aaccagatga tcgatatgtc catgggtaat tataatatat
     2641 tttatagaaa gactgtttgc ttctgccacc ctgattatct cattgggatc acacccggga
     2701 tcaataacaa ctgcctctcc tttgtcccca acaatataac agtttgaacc aaaaagtcca
     2761 tagggtatac attccagtat cataatatcc tccactcatt caaaagtaat agtatataaa
     2821 tataatccca aaaaccgtta ttaccttatt caaaacctaa aattccttct tgctgtccag
     2881 aaggatggta acaggtccgt tattatagat ctctaccatc atttcagcct gaaatttgcc
     2941 ggtctcaacc tttaaatcat aattcctgca aacctttaca aacctttcat aaaattcttc
     3001 cgccttcttg gctccggcag ccttgtcaaa gctgggtctt tttcctttcc tgcagtcgcc
     3061 gtaaagcgta aactgcgaca ctacaagaag gtcaccaccc acatccttta atgaaaggtt
     3121 catttttccg ttttcatcct caaaaatcct caggttcaca atcttgtctg caaggtattc
     3181 tatgtcccta tcagtgtcgt cgtcaccaac ccccaaaaga accgataaac ctttttggat
     3241 ttctcctatt gtctcgcctt caactgtaac cttagaagac gtcacccttt gtacaaccgc
     3301 tctcattaag atttcccctt tttaaatgtt tatctcctca ttattgcctg tttctggtga
     3361 cttcaaaaac cccgttaacc cttcttatct ttttaacaat tttctcaagc tgttctgtat
     3421 ttgttatctc caaagtcaag ttcataattg ctatctgatc cttggtagtc ctcgcattca
     3481 ttgccttaag cggaatcttg gattccccga taacattggt tatttccatc aaaagtgcag
     3541 ttctgtcatg ggccattact gtaatgtcag ccttgtatgc cacattgttt gaagtgtacc
     3601 agctgacctc aataagccgg ttatcaccat caatgttatt tgatatgttt atgcagtcct
     3661 tgcggtgtac cgacacaccc cttcctcttg tgatataacc tatgatatcg tcaccgggaa
     3721 cagggttgca acatctggag attctaacga gacagttttt aattccttta actataatac
     3781 cgttttcagg aacgcttttg gaagcagttt tcttcttttg ttcaaccgcc tcagacacaa
     3841 attctgtgat ttcatccagc ttgacacttt ttctgtattc ctctttcagc cgggaaataa
     3901 ctttgttggc agtcagtgca ccataaccga ttgcggcata tatatcatcc aaagtgccaa
     3961 aggtgtactt tcttaatata atctctatcc attcaggctt aaagagttga ttgtaggtaa
     4021 cagcctgctt tttcaactcc ctttcaatca tctctttgcc cctgataata ttttcttccc
     4081 ttttttcttt tttaaaccat tgattaattt tatttttggc ctgcgaactc ttaacgattt
     4141 tcaaccaatc acggcttggt ccttgtacat tggaagaggt cagtatttct ataatatctc
     4201 cgttctttaa gacatactcg atgggaacaa gcttgttgtt cacctttgca ccggacagct
     4261 tgtttccgat agcactatga atggaatagg caaaatctat cggggtcgag ccggtaggaa
     4321 gacttatcac atcccctttg ggagtgaaaa caaacacctc atcagtaaat agatctattt
     4381 tcaaggtctc cataaactca ttggcatccc gcatttcctt ctgccactcc aaaagctgcc
     4441 tcaaccacgc aagcttgttg tccgattctt tatcggaagt tccgccttgt ttatatcgcc
     4501 aatgagctgc aattcccact tcagctactt tatgcatttc ttccgtccta atctgtacct
     4561 caaagggaat cccttcatgc ccaattaaag tggtgtgaag ggactggtac atattaggct
     4621 ttggcatggc tatataatcc ttaaatctgc cgggcaaggg cttatatatt tcatgaacaa
     4681 gtccgagtac agcataacag tcctttacag aatgtacaat tacccttagt gcaaacaggt
     4741 caaatatttg atccaaggtt ttgttttgct gtatcatctt tctgtatata ctgtaaaaat
     4801 gcttgggccg gccctcaatt ttggcttcta ttccaatctc ggtagtcttc tctttaagag
     4861 tttcaattat tttggcaata aacgcttctc tctctcttct tttctgagct attttattta
     4921 ccaattcgta ataacccttt gagtctaaat acctgaaaca cagatcctca agctcccact
     4981 taatcttcga tataccaagc ctgtgggcaa gaggagcgta tatctccaag gtttcttttg
     5041 ctttttctat ttgcttttcg ggtgtcatat acttgagggt tctcatattg tgcaagcggt
     5101 cagccagctt tataagaata actctaatat cctttgccat tgcaagaaac attttcctta
     5161 aattctcggc ctgcagctct tcctttgagg tataaggaat cttacccagt ttggttactc
     5221 catccaccaa actggctact tcttctccaa actcctcctt aatctcctca taggtaaagg
     5281 tggtatcctc aatagtatca tggagtatgc ctgcaacaat agcagtactg tcaagctcca
     5341 aatcagccaa tataatggca acctccattg gatgcacaat gaaggggtcc ccggaaaccc
     5401 tctgctggcc ctgatgagcc tccagtgata tattgtgagc cttttcaata agattaaaat
     5461 cacagtttgg gttatattct ttaatttttc ttataagccg gttatagcca tcacccatta
     5521 agattccccc aa
//
LOCUS       DAOOJC010000118         5464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12006_length_5464_cov_3.804400, whole genome shotgun sequence.
ACCESSION   DAOOJC010000118 DAOOJC010000000
VERSION     DAOOJC010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12006_length_5464_cov_3.804400"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1199)
                     /locus_tag="PLA01_04570"
     CDS             complement(102..1199)
                     /locus_tag="PLA01_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00607.1"
                     /translation="MLGVVVLFKEAKDIFKVANIYMATIIGAGFASGQEIMQFFSAYY
                     EGGFFGILLAGALFAVIGYIVIVKVYTERIRNYEEFLFPTVGWVIGWIMEITVSLFMG
                     SVLCIMVAGAGSIISDKTGMPYQYAILAAAVLCMIAFLTDIKGIIVLSSFITPVLVLG
                     IIGIGLYIIISKDMSVFNIVSGFGTITHNWFVSSLLYVSYNSICAVVVLCSLLPHLKS
                     KRVAAAGGILGGVMLCFMAVIINIVLFIFYPNAASQEMPVLSIIDRYNSIAGEFYTVL
                     ILLAMFISGVTSGYGFIERISTKVKVSKKIIIPVICGFTIPLSGMGFSKLISLIYPVF
                     GYVGMFMVFAILLQGINMLNSAKKQRNTVFK"
     gene            complement(1324..2751)
                     /locus_tag="PLA01_04575"
     CDS             complement(1324..2751)
                     /locus_tag="PLA01_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HPD00608.1"
                     /translation="MKLYRNIVILVVVLVALIGAYLIVKPLVGTEEEVSTRIEVVEMD
                     SEKVVEVTIENSDGKFVFKKNEDQWVMISGGDFVIDTYRIDSIVSNVCDLYAYKIVEE
                     NPEDLGLYGLDKPVLVSVKTSEGETVEIEVGNLTATKQAYYMKKKGENTVYTIPDFAG
                     EVLSSRKTDLRNKYVLDVTSSEVTELVLTKDGKVTFNAVKVPDSGWTIKEPIESGVDM
                     IRLNNALEELVRAEVIDYIEEDAKDLSKYGLDEPSYIVKAAAGNLKVTLLLGDVKENY
                     KEVYGMFEGTNEVFVINPNTLGFLDITSVEIIDGFIYAPYIYDVTDIEVKMDGETINL
                     KIEKIEDTSSGGDEQTSGQDSEANFYVNGRNVNEKGKDGISKFKSYYQSLISITADDI
                     DPNALVEGEPEISITYKLNKAPGEVKVDLIPRDEYTYYAMKNDKYTGIVINRSGLDEE
                     AGPRKSYQNLIELLNSEDQATDRQE"
     gene            complement(2748..4148)
                     /locus_tag="PLA01_04580"
     CDS             complement(2748..4148)
                     /locus_tag="PLA01_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HPD00609.1"
                     /translation="MKNVKYSTLFLIMAVLVIGITIFINVLAATVDIKWDMTTNKLYS
                     IGEQTETILNNLQKEVEIIMLSDKEEIRTEQAGFILVEFLENYNKFDKVNVSFIDPDR
                     NPNIVTKLDESGVLTLNAHDIIIKCGSKSKKITLYDIFQQDNYGLMMFYGEQAVTGAI
                     KYVISDATPTIYFVDSNTARKLDSQYTIIKGSFENNNFLVKKINLTASDKIPEDCTIL
                     FFTAPNKDLSQNEKNIVSQYLKNGGNAVFLFDPISSSEKFKNFDSLLEEYTIALDYDR
                     VKENHPQMYVANRPYDIIPMLQACDVTNGLDLSQQTVIMPSSMSFKRLLNDKEPLTVM
                     PLLTTSDAAVGEPYGGGVTEEIAGPVDVGLIAQYDSINTTKIAVIGNAYFATDEIISY
                     YYPDSYSTLTLMARTVDWMYDKTNDVFVLPKSSNLDSIILTGLNASLIVVVTVIAFPI
                     LIVAAGIIIWLRRRHL"
     gene            complement(4155..4871)
                     /locus_tag="PLA01_04585"
     CDS             complement(4155..4871)
                     /locus_tag="PLA01_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPD00610.1"
                     /translation="MLSIYKRELRTYFYSPLAYILSGIFVVVFALNFLSQVISENGIS
                     RFIFGGQLYFASFFLVMLIPILTMRTFAEERRNGTEVLLMTSPVSVPQIVIGKYLAAL
                     TVFLTMTACSLIFPAIIMIKGDLFVSIAASGYIGFILLGMAFVAFGLFTSSITESQII
                     AAVLGCISLFVLLIMDQVKGITTGIIRTVLNSISVYEHYKEFVQGMISLQDVLFFLSV
                     SAMFLALIILVIEKRRWSQG"
     gene            complement(4875..>5464)
                     /locus_tag="PLA01_04590"
     CDS             complement(4875..>5464)
                     /locus_tag="PLA01_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HPD00611.1"
                     /translation="RVGVAQALIGNPEVLILDEPTVGLDPNQILEVRNVIKDLGKEHT
                     IILSTHIMQEVKAVCERVVIIDKGKIVAVDTPENLSKSISSSSRMVVTIAGPKKSVLD
                     AFKSIYGVKRADVLKKKDEDVFDYVVDSDKEIDVRKTIFFEFAKLGYPIMEFRTEEMD
                     LEEVFRELTTKNIKLNNEGLKEDASKGDEEEKEVE"
BASE COUNT         1656 a         1262 c          809 g         1737 t
ORIGIN      
        1 tcaaataaca atccgaattc ttttctatag acattgtacc ataaacagtg ttttttcatc
       61 aattattgtt ttgtgtcatt tatttagccc gaaaggttat cttacttgaa tacagtattt
      121 ctctgtttct ttgcagaatt taacatgttt ataccctgca gcaaaatcgc aaataccata
      181 aacatcccca catatccaaa caccggatat atcagagaaa taagctttga aaaacccata
      241 cccgataaag gaattgtaaa cccgcatata accggtatta ttattttttt actcacttta
      301 accttcgtac ttatcctctc aataaaacca taaccggaag taactccgga aataaacatt
      361 gcaagcagta ttagaacggt ataaaactct cccgctatgg agttgtatct gtcaataatg
      421 ctcaggacag gcatctcttg agatgctgca ttggggtaaa aaataaaaag gacaatattt
      481 ataattacag ccataaagca gagcatgact ccaccgagta ttcctcccgc agctgccacc
      541 cttttgcttt ttaagtgcgg taaaaggctg cacaacacta caactgcaca tatgctgtta
      601 tagcttacat agagtaagga tgaaacaaac caattgtgcg ttattgtgcc aaatccggaa
      661 actatattga ataccgacat gtctttactg attattatat ataacccgat accgataatt
      721 cccagtacta aaaccggcgt tataaacgag cttaaaacaa ttataccttt gatgtcggta
      781 aggaaagcta tcatgcacag aacagccgca gccagtatgg catattgata aggcatccct
      841 gttttatcag atattatgct ccctgcacca gcaaccataa tacacaagac tgagcccata
      901 aaaagcgata ccgttatttc cataatccac ccgataaccc accccactgt gggaaacaga
      961 aactcttcat aatttcttat tctttcagta tacactttaa caattactat atatccgata
     1021 accgcaaaca aagctcccgc caaaaggata ccgaaaaaac cgccttcata gtaagcagaa
     1081 aagaactgca tgatttcctg tcccgaagca aagcctgcac caattatagt tgccatatat
     1141 atatttgcta ccttgaatat gtcttttgcc tctttaaaca aaacaacaac tcctaacata
     1201 gaattatatc taattaattc tatgttggag ttgtcttaca aattacaagc atttgtctat
     1261 tgcagcagaa cttgctgctt tgtttttttt gttttatctc ccggtataca tttaccggtg
     1321 catttactct tgtctatcgg ttgcctgatc ctctgaattc agtagttcaa taaggttctg
     1381 ataggacttc cttggtcccg cttcttcgtc caaaccactc ctattaataa caattcctgt
     1441 gtatttgtca ttcttcattg cataataggt atattcatct ctcggtatga gatcaacctt
     1501 cacctcgccc ggtgctttgt tcagtttata ggtaatactg atttcaggtt caccctctac
     1561 caaagcgttc ggatctatat catcggccgt aatgctgata agtgattgat aatagctctt
     1621 aaatttagat atgccatcct tacccttttc attcacattt ctgccgttga catagaaatt
     1681 cgcttctgag tcttgcccgc tcgtttgctc atccccgcca ctacttgtat cttcaatttt
     1741 ctcgattttt aggttaattg tttctccatc cattttgact tctatatcgg tcacgtcgta
     1801 gatatagggt gcatatatga aaccgtctat tatttcaacc gatgttatat ccagaaagcc
     1861 gagggtattc gggtttatga caaacacctc atttgtgcct tcaaacattc cgtagacttc
     1921 tttataattc tccttaacat cccctaaaag cagggttacc ttcagatttc ctgcagcagc
     1981 cttaacaatg tatgaaggtt catcaagccc atacttgctc aggtccttag catcctcctc
     2041 aatatagtct attacttctg cccttacaag ctcctccaat gcattattca acctgatcat
     2101 atcaacaccg gattctattg gctcttttat agtccaaccg ctgtctggta ctttaactgc
     2161 attaaaggtt accttaccgt ctttagtcaa caccagttcc gttacctctg aggaagtcac
     2221 gtccaaaaca tacttgtttc tgagatccgt cttgcgtgat gacaatactt cgccggcaaa
     2281 gtcagggatt gtataaacag tgttttctcc ctttttcttc atatagtacg cttgcttagt
     2341 tgccgttaag ttacccacct caatttctac tgtctcaccc tccgatgttt taaccgaaac
     2401 taagaccggt ttatccagac cgtacaaccc aagatcctcg ggattctctt ccactatttt
     2461 ataagcataa agatcacaaa catttgaaac tatgctgtca atacgatacg tatcaatgac
     2521 aaaatctcca ccggaaatca taacccattg atcttcgttc tttttaaata caaacttccc
     2581 atcgctgttt tcaattgtca cctctactac tttttcgcta tccatttcta caacttcaat
     2641 tctcgtacta acttcttcct cagtacccac cagaggcttc acaataaggt atgcacctat
     2701 taaagcaacc agtacaacta caagaataac aatattcctg tataatttca tagatgcctc
     2761 ctcctcaacc aaataattat acctgcagca acaattagaa tcggaaatgc aataacagtg
     2821 acaactacaa ttagacttgc attaagtcct gtaagaataa ttgaatccaa attggagctt
     2881 ttgggaagaa caaacacatc atttgtctta tcatacatcc agtctacagt acgggccata
     2941 agcgtcaaag tactgtacga atcaggataa tagtatgata tgatctcgtc agtagcaaaa
     3001 taagcatttc caatcactgc aattttcgtg gtatttatac tatcatattg agcaataagc
     3061 cctacatcca ccggtcctgc gatttcctct gtcactcctc caccatacgg ttctccaaca
     3121 gcagcatcac ttgtagtcag aaggggcata acagttagag gctccttatc attgagaagt
     3181 cttttaaagc tcatggatga cggcattatt acagtctgct ggctcaaatc caatccgttg
     3241 gtaacatcac atgcttgaag cataggaatt atatcatacg gtctgtttgc tacatacatc
     3301 tgcggatggt tttcttttac tctgtcataa tctaatgcaa tagtatactc ctcaagaagg
     3361 ctgtcgaaat tcttgaattt ttccgatgaa cttattggat cgaataagaa cacggcattt
     3421 ccaccgtttt ttagatattg acttactata tttttctcat tctgagacaa atccttattg
     3481 ggtgctgtaa aaaacaaaat tgtacaatcc tccggaatct tatcagaggc tgtaagatta
     3541 attttcttta ctagaaaatt gttgttttca aaggaaccct taatgattgt atattgggaa
     3601 tcaagctttc ttgcagtgtt tgaatccaca aagtatattg tcggcgttgc atcgctgata
     3661 acatacttaa ttgctcccgt cactgcctgt tcaccgtaaa acatcatcaa tccgtagttg
     3721 tcttgttgga agatgtcata caaagtgatc ttcttacttt tagaaccgca ttttataata
     3781 atgtcatgtg cattaagcgt aagcacaccc gactcatcca gtttagtcac aatattcgga
     3841 ttcctgtccg gatctataaa gcttacattt actttgtcaa atttattata gttttccaaa
     3901 aactcaacca aaataaaacc tgcctgttcg gttcttattt cttctttgtc gctgagcatt
     3961 attatttcta cttctttttg aagattatta agaatagtct cggtctgttc tccaattgaa
     4021 tagagcttgt tcgtagtcat atcccattta atatctacgg ttgctgccaa aacatttata
     4081 aatatagtaa ttccaattac aagaacagcc attataagaa acaacgtact gtattttaca
     4141 ttcttcatca tttatcaccc ctgactccat cgcctttttt ctataaccaa aattatcaat
     4201 gcaagaaaca tcgctgatac acttaagaaa aacaaaacat cctgaagact aatcataccc
     4261 tgaacaaatt ccttgtaatg ttcatacacg gaaatcgaat ttaaaactgt tctaataatt
     4321 cccgttgtaa tgcctttgac ctgatccatg attaagagaa caaacaggct tatacatcct
     4381 aatactgctg caattatttg actttcggta atagaagacg taaataatcc aaatgccaca
     4441 aatgccatac ccaaaagtat aaatcctata taacccgatg ctgcaatgga aacaaataag
     4501 tctcctttta tcattattat cgcaggaaaa atcaaactgc atgcagtcat tgttaaaaac
     4561 accgttaatg ctgcaaggta tttgcctatt actatttgag gaacactaac cggtgatgtc
     4621 atcaataata cctcagttcc gtttcttctt tcttctgcaa aggttctcat agtcaaaatc
     4681 ggaatcaaca taacaagaaa gaaactcgca aagtaaagct gtcccccaaa aatgaacctc
     4741 gaaataccgt tctcggatat aacctgactt aaaaagttca atgcaaacac gacaacaaat
     4801 atacccgata gaatatatgc cagcggggag taaaaatagg tacgcagctc ccttttataa
     4861 atagacaaca taaattattc aacctccttc tcttcctcat cacccttcga agcatcttcc
     4921 ttcaagcctt cattattcaa ctttatgttt tttgttgtaa gctcacggaa tacttcctca
     4981 agatccattt cttcagtcct gaattccatt atcggataac ccagttttgc gaattcaaag
     5041 aatatggttt ttctgacatc aatctcctta tcggagtcca caacgtaatc aaagacatct
     5101 tcgtccttct ttttaagaac atcagcacgc ttgacaccat atatgctttt aaaggcatcc
     5161 agcactgatt ttttgggtcc tgctattgta actaccattc ttgacgaact ggaaatgctc
     5221 tttgaaagat tttccggcgt atctacagcc acaatcttac ccttgtctat tataacaaca
     5281 cgctcacaaa cagcctttac ttcctgcatt atatgggtac ttaatattat tgtatgctcc
     5341 tttccaagat ccttaataac gttcctgact tcaagaatct gattcgggtc aagacctaca
     5401 gtaggctcgt ccagtattaa cacctccgga ttgcctatga gggcctgggc aactcccacc
     5461 ctct
//
LOCUS       DAOOJC010000119         5446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12052_length_5446_cov_4.071786, whole genome shotgun sequence.
ACCESSION   DAOOJC010000119 DAOOJC010000000
VERSION     DAOOJC010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12052_length_5446_cov_4.071786"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PLA01_04595"
     CDS             complement(<1..849)
                     /locus_tag="PLA01_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD00612.1"
                     /translation="MSKRVIIAVTLLLLILLTSCSSAESYNNKGKNLLEKGEYEEAIN
                     YFDKALEKNPESERTYSNKGLALYKTGKIEEALECLDKSIKLNPQFPVAHKTKGNVLF
                     AVSKFEEALECYNKAIELDSKYAQCYSDRGVLYYKMNRLQPALEDLDKAMELGLKAGN
                     EYNTRGLILDKMKNYQEALEMFDRAIELEPDNSTVYSNKALTLWNAGDRSGAISCVNK
                     SIELDDKNAAAYANKGFILWKMQNNEEAYKCLFKATELNIGYWDAYNDLGLMLLEEGD
                     SRRAIEM"
     gene            complement(1041..2606)
                     /locus_tag="PLA01_04600"
     CDS             complement(1041..2606)
                     /locus_tag="PLA01_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPD00613.1"
                     /translation="MKFSEKAKFFLAGMITTAIIATSLTAYTMSMRKTITVDYKDIKI
                     VVDGKLIDPRDGNGRKVEPFIYEGTTYLPVRAVSEALGKYVRWDGDTYTVQILSNPIE
                     DNERNKYETVLDVWIMPNSQQPIEDFIKTASPFLQDNPDIGLNVSIIDWGSAWTRLME
                     ASLTGEEAPDITQLGTMWVATFSDRGLLADITEDISWDEFHPQTLGSTGIAREKGKFA
                     VPWFADTRALFYRIDACEKAGVDPTKDFQTWDSFKEALKKLNNVEINGKKLSALGMPG
                     KNDWNVVHNYAPWIYGAGGSFLNLDFTKSALSSQDTFDGIKFYSELAFEGLMDKEALE
                     KDTNSIETAFINGDYAISIMGPWNINTLEKNKNNNENALIDKIGVAILPKGPKGRFAY
                     LGGSSLAVVNSSDKKEEAVELLKWLTGKEAQVEYSKTTGNLPVVKSAYEDPWIKDNRL
                     RKVFADQMNFAIEYPSIPAWPFMESYLQMALSEVWDNVMQVNGEYSPDKTMEALKQSE
                     RQINMVIEEHNEF"
     gene            complement(2777..3967)
                     /locus_tag="PLA01_04605"
     CDS             complement(2777..3967)
                     /locus_tag="PLA01_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPD00614.1"
                     /translation="MLEIKGKYNTAKVFTDNVEGDAIAQIIELCNQEFAQDSIIRIMP
                     DTHAGAGCTIGTTMTIRDKIVPNLVGVDIGCGMETIKLKNKNIELSKLDKVIHEYVPA
                     GFDIRQKEHPYAKNIDLEKLICKKNVNINRAKLSIGTLGGGNHFIEVNKDKDGNLYLV
                     VHSGSRHLGKQVAKFYQELGYKELVTQSEIINELVAKMKEEGREKEIQKEIKKIKPPN
                     ISKHLAYVQGISFDNYLSDMKLVQKFAVLNRKAILDEIVRRMDFRIEEQFTTIHNYID
                     LDNMILRKGAISAREGEKVLIPINMRDGSLICIGKGNKDWNFSAPHGAGRLMSRSKAK
                     EVISLSEFKKSMEGIYSTTVNRSTIDESPMAYKPMDEIIENVRDTVEITDIIKPIYNF
                     KAAE"
     gene            complement(4262..5329)
                     /locus_tag="PLA01_04610"
     CDS             complement(4262..5329)
                     /locus_tag="PLA01_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HPD00615.1"
                     /translation="MQKKKPALLAAVLTFLIVCLTVNSALVFAASGVLKEGMSGNDVT
                     SLQQDLKTLGFFNEEPTGYFGNITKEAVIKLQAQQGLEKDGIAGPKTFSVIENLLNQR
                     TATRSSGGSSLKEGMSGNSVTSLQQDLKALGYLSVNPTGYFGDLTKEAVKKLQAKHGL
                     EQDGIAGPKTLSTIDRLMGRTGVTSTATRGNLGSKDYMISWYGNAENVFSIGATAQVY
                     DIRTGRTFNIKRSYGYNHADCETLTANDTKIMLEIYGGSWNWERRPIILTVNGRKLAA
                     SMAGMPHAGVDSAPANTYVKSRSGGYGGGDNLDSVKGNNMNGVFDVHFLNSKTHGTSR
                     VDSNHQQAVKEAASWAEKNGI"
BASE COUNT         1368 a         1335 c          900 g         1843 t
ORIGIN      
        1 catttcaata gcccttctgc tatctccctc ctcaagcagc attaaaccca aatcgttata
       61 ggcatcccaa tacccaatat tcaattcagt tgccttaaac aaacatttat aagcctcttc
      121 attattctgc atcttccaaa gaataaaccc tttgtttgca taagcagcag catttttgtc
      181 atccaattca attgacttgt tgacacaact tattgccccg cttctatccc cggcattcca
      241 cagggtcaat gccttattgc tatagacagt actgttgtcc ggttccaact caatggcacg
      301 gtcaaacatc tcaagagctt cctgataatt tttcattttg tccagaataa gacctcttgt
      361 gttatattca tttccggctt tcaagcctag ctccattgcc ttatccaaat cttccagagc
      421 cggctgaagc ctgttcattt tgtaatacag aactcctctg tcactatagc attgtgcgta
      481 cttggaatcc aattcgatag ctttgttgta acattccagt gcttcctcaa attttgaaac
      541 ggcaaaaagt acatttccct tggttttgtg agcaacagga aactgaggat tcagcttaat
      601 tgacttgtcc aggcactcca aagcttcctc gatcttcccc gttttgtaca gggccaggcc
      661 tttattactg tatgttctct ccgactcagg atttttttcc aatgccttat caaaatagtt
      721 tattgcctct tcgtattcac ctttttcaag aaggttctta cctttattgt tataggattc
      781 tgcactgctg caggatgtca aaagtatcag cagcaataag gtcacagcta taataaccct
      841 ttttgacatc aatttttgcc ccccaggtaa atgcatattt gcagtttata agctattgaa
      901 catatctatt attatagcag gattttcctt cagcatcaat tatatgggca ggagtgggtg
      961 tattaaaaaa agagccatat ttatggctct ctttttagta tgttttgtaa ataattaaca
     1021 ttattctgct tgtctaactt ctaaaattca ttatgttcct caataaccat atttatctgc
     1081 ctttcactct gttttaatgc ttccatggtt ttatcgggac tatattcacc gttaacttgc
     1141 ataacattat cccatacttc agacaatgcc atttgtaaat acgattccat gaaaggccat
     1201 gccggtatgg acggatattc tatcgcaaag ttcatttgat cggcaaatac ttttctcagc
     1261 ctgttgtctt ttatccatgg atcctcatat gctgatttga ccacaggcag gtttcctgtc
     1321 gttttactgt attctacttg ggcctcttta cctgtcagcc attttaaaag ttccacagct
     1381 tcctccttct tgtctgagct gttgacaact gcaagtgaac ttccaccaag atatgcaaat
     1441 ctaccctttg gaccttttgg aagaattgca actccaatct tgtctatgag tgcattttca
     1501 ttattgttct tatttttttc caatgtatta atattccatg gacccataat agaaattgca
     1561 taatcaccgt taataaatgc tgtttctatg ctattagtgt ctttttcaag agcctcttta
     1621 tccattaacc cctcaaacgc cagctctgaa tagaatttaa ttccatcgaa agtatcctgg
     1681 ctgctaagag ctgattttgt gaaatccaaa ttgagaaaac tccctcctgc cccgtaaatc
     1741 cacggtgcat aattgtgaac aacattccag tcgtttttac ccggcattcc cagagcagac
     1801 aatttcttgc cgttgatctc cacattgttc agttttttca aggcttcttt gaaggaatcc
     1861 caagtttgaa aatcttttgt cggatcaacc cccgccttct cgcatgcatc tatcctgtaa
     1921 aataacgctc ttgtatctgc aaaccaaggc acagcaaact ttcccttttc cctcgcaatt
     1981 ccggttgaac ccagtgtctg aggatgaaat tcatcccaac ttatatcttc cgtaatgtct
     2041 gccaaaagac ccctatcgga aaaagttgct acccacatgg ttccaagctg ggttatatcc
     2101 ggtgcttctt ctcccgtcaa agatgcctcc attaatctag tccacgcact tccccagtct
     2161 attatcgaaa cattcaaacc tatgtcagga ttatcctgca gaaaagggga tgcagtctta
     2221 ataaaatcct ctataggttg ctgagagttt ggcataatcc aaacatcaag aactgtttca
     2281 tacttattcc tttcattgtc ttcaattgga ttggataaaa tttgtacggt gtatgtatcc
     2341 ccatcccatc tgacatattt cccgagagct tcactcacag cccttacagg caagtatgtt
     2401 gttccctcat agataaacgg ttcaaccttc ctgccatttc catccctagg atcaatcaat
     2461 ttcccgtcaa cgacgatttt aatgtcctta taatcaacgg ttattgtttt tctcatagac
     2521 atggtgtaag ccgttagaga tgtagctatt attgctgttg taatcattcc tgcaagaaag
     2581 aattttgctt tttcagaaaa cttcatttgt atctcacctc aatttctcta ttatgttaat
     2641 atccacataa taaatatatc ataccaattt atccatttca aataaatact cccagacttc
     2701 acacttattt aatgcaccct gtcataataa acacagactt tctttaaact ttggatatac
     2761 accgacaaat ctatactcac tcggctgcct taaagttata tataggtttt ataatatctg
     2821 tgatttcaac ggtgtccctg acgttttcaa taatctcgtc cataggcttg taagccatgg
     2881 gagattcatc gatagtcgac ctgttgaccg ttgttgaata aatcccttcc atggattttt
     2941 tgaactcact gagggatatt acttccttcg cttttgacct gctcatcagc ctgcctgcac
     3001 catggggtgc agaaaaattc caatccttat tgcctttccc gatgcaaata aggctcccgt
     3061 ccctcatatt aatgggaatc aggactttct ccccttctcg ggctgaaatc gcacctttcc
     3121 gaagaatcat attgtcaagg tcgatatagt tatgtatagt ggtaaactgc tcctcaattc
     3181 tgaagtccat acgcctaaca atttcgtcca aaatcgcctt gcggtttagt accgcaaact
     3241 tttgcaccag tttcatatcg gaaagataat tgtcaaagga tataccctgg acataggcaa
     3301 gatgtttgct gatgtttgga ggcttaatct tctttatctc cttttgaatc tctttttccc
     3361 gtccttcttc cttcatcttt gccacaagct cattaataat ttcactttgt gtcaccagtt
     3421 ccttgtagcc aagttcttga tagaatttgg caacctgctt cccaagatgc cggcttcccg
     3481 aatgtacaac taggtataga ttaccatcct tgtccttgtt tacctctata aagtggttgc
     3541 cgccccccag cgttccgatg cttagcttcg cccggtttat attaacattc tttttgcata
     3601 tgagtttttc cagatcgatg tttttagcat agggatgctc cttttgccga atatcaaagc
     3661 ctgccgggac atattcatgg atgaccttat ccagcttgct cagctcaata tttttatttt
     3721 taagctttat ggtctccatt ccgcagccaa tgtccacacc caccagattg ggaacaattt
     3781 tatctctaat tgtcatggtt gttccaatgg tacatcctgc accggcatgg gtatccggca
     3841 taattcttat tatgctgtct tgtgcaaatt cctggttgca tagttctatt atctgtgcaa
     3901 tagcatctcc ttctacattg tcggtaaaga ccttagccgt attgtatttc cctttgattt
     3961 ctagcataac ccccaccgtc tttcagttta ctatttatct attctttttc ttatataatt
     4021 atttgcagca tacaacagca taagttattt ttcaaatact atcatatctt tttcccccgg
     4081 ttcaacatat acatctgctg caaaatggtt atcatcttca tctatggtaa ttttagtgtg
     4141 ttgatcattt cacttcgtga aatcccacta aaaaggagct acgcactaat tgatccgtgc
     4201 cgtagcccct taacctgcaa tatttgtcac ggaagaaccg tccccccgtg tcccccttgt
     4261 gtcagatgcc gttcttctca gcccaacttg cagcttcctt tacagcctgt tgatggtttg
     4321 aatccacacg gctggttcca tgggttttgc tgtttaagaa gtggacatca aaaacaccgt
     4381 tcatgttatt tccttttacg gaatcaaggt tatccccgcc tccgtaacct ccacttctgg
     4441 atttgacata tgtattggcc ggtgcactgt cgacacctgc atgaggcatt ccagccattg
     4501 atgcggctaa cttacgcccg tttactgtga gaattatcgg ccttctctcc cagttccaac
     4561 tgccgccgta tatttcaagc attatttttg tgtcgttggc ggttaaagtc tcacagtcag
     4621 catggttata gccatatgat ctctttatgt tgaatgtgcg tcctgtcctg atatcgtata
     4681 cctgtgcagt tgctcctata ctaaatacat tctcggcatt accgtaccat gaaatcatat
     4741 aatccttgct tccaagattt cccctcgttg cggttgatgt aacgcctgtc cttcccatga
     4801 gtctgtcaat ggttgataaa gttttaggtc ctgcaattcc atcctgttca agcccatgct
     4861 ttgcctgaag ttttttcacc gcttcttttg tcaggtctcc aaaataccct gtcgggttta
     4921 cacttagata tcccagtgcc ttaaggtcct gctgcaaaga tgtcacgctg tttccgctca
     4981 ttccctcttt taatgaggaa cctcccgaag accgggtggc agtcctttga ttaagcaggt
     5041 tttcaataac cgaaaaagtt ttcggccccg caattccatc cttctcgagt ccttgctggg
     5101 cttgaagctt aattaccgct tcttttgtta tgttgccgaa ataccctgtg ggctcttcgt
     5161 taaaaaaacc cagtgtctta aggtcctgtt gcagtgatgt aacgtcattt ccgctcatcc
     5221 cttccttcaa aacacccgaa gcagcaaaaa caagtgcaga attaacagtc aggcatacta
     5281 taaggaaagt cagaactgct gctagcaacg caggcttttt cttttgcatt tacattcgtc
     5341 ccccctcttc atcttggatt cagcttttac tatatccttc acaattgtaa cagtcgagta
     5401 aacccgggga ctttcacccc gagcttctca cagaaccgta cgtgat
//
LOCUS       DAOOJC010000120         5422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12113_length_5422_cov_3.343954, whole genome shotgun sequence.
ACCESSION   DAOOJC010000120 DAOOJC010000000
VERSION     DAOOJC010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12113_length_5422_cov_3.343954"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PLA01_04615"
     CDS             complement(<1..858)
                     /locus_tag="PLA01_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPD00616.1"
                     /translation="MKKYLSLILVASFLIALLTPWTTISAEPDFNYGEALQKAIMFYE
                     FQRSGELPPTIRNNWRGDSCLDDGKDVGLDLTGGWFDAGDHVKFNLPMAYTAAMLAWA
                     VYEEKEAFVKSGQLGYILDEIKWATDYFIKCHPEPDVYYYQVGDGDADHMWWGPAEVI
                     HMERPVFKVDLGSPGSTVSAETAAALAAASIVFKDTDSSYSNLCLKHAKELFSFADKT
                     RSDAGYTAASNFYKSHSGFYDELTWAATWIYLATNDESYLDKAESYIEFWSTEPQTDI
                     MSYKWGHCWD"
     gene            1070..4309
                     /locus_tag="PLA01_04620"
     CDS             1070..4309
                     /locus_tag="PLA01_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPD00617.1"
                     /translation="MNNFDIPDSIIYQYASSNETYKKGLLYNSNHRVLNFYFNPEKMA
                     VRAVVRGTHDYDVHIYFDNIGRVKQYECDCPAYYTYYGACKHVVAVMKKAQSEIKNYI
                     RKNNQNEAINDVFSFFDGLTSHNHKQEVQLEITYDFQRDYHNRYISSMEFRIGTGRLY
                     VLRNIKEFLAKVYDSQPFEFGKNFIFDPAKHAFLGANKEIIDILLEIYENEKLLEQRF
                     RYTYEASASLFSGKKLTLSTPYLLRIFKALGNTSFYAKILDSGVNTTSIVEDDLPLNF
                     SLSQQNNNILFSLQSDTIVTPLTEKGIYFYCGGKIYHPSEKQQKLYLPFISRFFDKRN
                     GGLYFPNTHAERFVSEVLPHVRKIGSLKMDPEIEKKLVNENLVSKIYFDKYRDGISAV
                     INFHYGENTVNPFSSFNKNYKSSDKIVLRDVESEKNIISLFEASEFVVDKDRLYLDDE
                     EKIYVFVNNILPKLQELSEVYYSDDFKNINVKYPSGFSGGIRLNQESNILEFSFEYED
                     ISHEELADIFSSIKEKKKYFRLKDGSFLALDTPEMERMSDLIDKLDITGKDLSKKVIE
                     LPKYRAMYLDSLLEESELMGVERDTNFIRMVQNIKQPGSLDFEIPEKLKNILRDYQKV
                     GYKWLKTLSAYGLSGILADDMGLGKTLQIISFVLSEKDSAKGPSLVVAPTSLIYNWQN
                     EVKKFVPELNVLVVSGLPSERKELLLQAGNADLVVTSYPLLRRDIGLYKDIKFQYCFL
                     DEAQHIKNPNTLNAKTVKQIKSEMNFALTGTPIENSITELWSIFDYVMPGYLLSHPKF
                     VKKFEVPIMKHNDKNALKDLSKHISPFILRRLKKDVLSELPDKIESKLTCEMTPEQKK
                     IYLAYLQKSKKEISLELESRGFEKSHIKILALLTRLRQICCHPSMFIENFKGESGKMQ
                     LLEEILSSAFDSGHRVLLFSQFTGMLEIIKQSLNKQGIEYFYLDGSTKAQDRLEMVDS
                     FNSGSKNLFLISLKAGGTGLNLTGADMVIHFDPWWNPAVEDQASDRAHRIGQKNVVQI
                     MKLITRGTIEDKIFELQQKKKEMIDSVIKPGETLLSKMTESEIRELFEL"
     gene            complement(4439..4957)
                     /locus_tag="PLA01_04625"
     CDS             complement(4439..4957)
                     /locus_tag="PLA01_04625"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPD00618.1"
                     /translation="MEKTNIVLIGMPGAGKSTIGVLLAKALKKPFIDTDLLIQQKYNM
                     FLQDIIDKYGIKRFLSVEEEIAVSMDIRNHVIATGGSIIYSDAALKHLKKEGFVVYIK
                     LRLDEIEKRISNISSRGIAKEKDKTLMDLYNERVPLYEKYADITVNCSDMDIEASVAE
                     IINRIKTTEKFF"
     gene            5159..5389
                     /locus_tag="PLA01_04630"
     CDS             5159..5389
                     /locus_tag="PLA01_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00619.1"
                     /translation="MDQNKNDVINGESLNIDATLEKLSRELKADETLILDIGNNVLGQ
                     TDTIYDTLVTKGYDVRKSFKNGRNQILVSKKH"
BASE COUNT         1768 a         1084 c         1008 g         1562 t
ORIGIN      
        1 atcccagcaa tgtccccatt tgtatgacat tatatcggtt tgaggctcag tactccaaaa
       61 ttcaatatag gattcagctt tatcaagata gctctcgtca ttagtcgcaa gataaatcca
      121 tgtagccgcc catgtcagct catcatagaa tccgctgtgg gatttgtaga aatttgatgc
      181 cgctgtataa cccgcatcac ttcttgtttt atctgcaaag ctaaacagtt cctttgcatg
      241 ctttaaacac agattggaat aactggaatc ggtgtcttta aaaactattg atgccgcagc
      301 caaagctgcc gccgtttctg cagataccgt tgaacccggt gaaccaagat ccactttgaa
      361 caccggcctt tccatgtgta taacctcggc aggaccccac cacatatggt cggcatctcc
      421 gtctcctact tgatagtagt atacatccgg ctcaggatga catttaataa aatagtcggt
      481 tgcccattta atctcatcca aaatatatcc cagttgtccg ctcttaacaa aagcttcctt
      541 ttcctcatat actgcccacg ccagcattgc agcagtatat gccatgggca ggttgaactt
      601 tacatggtca ccggcatcaa accaaccgcc cgtcaggtca agccccacgt ccttaccgtc
      661 gtcaagacag gaatcccctc tccaattgtt cctgatggta ggaggaagtt ctcccgaacg
      721 ctgaaattca tagaacatta tggccttttg caaagcctct ccataattaa aatcaggttc
      781 tgccgatatt gtagtccaag gagtcaacag tgcaatcaga aacgaagcaa ctaaaataag
      841 gcttaaatac tttttcattt tcttctatct cccctttata caatctttct ctgtatatat
      901 attaatgcgt aaatacacca tatttttaat gggtatggct tggaaatctc aaaaaaaatt
      961 atgtaatcca tagcataaaa acatttatat tttttgtttt aattatactg taattgttat
     1021 atacttatat gttgaacaat taagataaat ttgaggtgtt tatatgaatt tgaataattt
     1081 tgatattccg gatagtataa tataccagta tgcttcgtcc aacgagacct ataaaaaggg
     1141 cttattgtat aattctaatc acagggtact aaacttttat ttcaatccgg agaaaatggc
     1201 agtaagagca gtagtaagag gtacacacga ctatgatgtt catatatatt ttgataatat
     1261 cggcagagta aaacagtatg aatgtgattg tcccgcttac tacacttatt acggtgcctg
     1321 caagcatgtc gttgccgtaa tgaaaaaagc tcaaagcgag ataaaaaatt atatccgcaa
     1381 aaataatcag aatgaagcca taaacgatgt attttccttc tttgacggtt tgacaagtca
     1441 taaccataag caagaagtcc agttggaaat aacctatgat tttcaaaggg attatcataa
     1501 cagatacatc tcttcaatgg aatttcgaat cggtaccggc aggttatacg tattaagaaa
     1561 cataaaggag tttcttgcaa aagtgtatga cagccagccc ttcgagtttg gaaaaaactt
     1621 catctttgat cctgcaaaac atgctttttt gggtgctaat aaagaaataa tcgatatttt
     1681 actggaaatt tacgaaaatg aaaaactatt ggagcagagg ttccgttaca cttacgaagc
     1741 ctcagccagt ttgttctccg gaaaaaagct aacactgtcc accccttatt tgcttagaat
     1801 ttttaaagct ttagggaata cttcgttcta tgccaaaatc ctggatagtg gggtcaatac
     1861 tacttcaatt gttgaagatg accttcccct taatttttcg ctgtcacagc aaaacaataa
     1921 tatactgttt tccctgcaat cggatacaat tgtaacgccc cttacggaaa aaggcattta
     1981 tttttactgt ggcgggaaaa tataccatcc atctgaaaaa cagcaaaaat tataccttcc
     2041 gtttatttca aggttttttg acaaaagaaa cggtggctta tatttcccaa atacccatgc
     2101 agaacggttt gtatcggaag ttcttcccca tgtacgcaaa ataggttctc tcaagatgga
     2161 tcccgaaatt gagaaaaagc tggtcaatga aaaccttgtt tcaaagattt attttgacaa
     2221 atacagagac ggtatttccg ccgttattaa tttccattac ggcgaaaaca cggtaaaccc
     2281 cttttcaagt tttaataaaa actataaaag cagtgataaa attgtgctta gggatgttga
     2341 gtccgagaag aacattatta gcctttttga ggcatcggag tttgttgtgg ataaagacag
     2401 attatacctt gatgatgagg agaaaatata tgtgtttgtc aataacattc ttcccaagct
     2461 gcaggaattg tctgaggtat attattctga tgactttaaa aacataaacg taaagtaccc
     2521 ctccggcttt tcgggaggaa taaggcttaa tcaggaatca aacatactgg agttttcctt
     2581 tgaatatgaa gatatcagtc atgaagagct ggctgacatt ttttcctcca taaaggagaa
     2641 aaagaaatat ttcaggctca aagacggttc ttttttagct ttggataccc ctgaaatgga
     2701 aagaatgagt gaccttattg acaagcttga tattacggga aaggatctct cgaaaaaggt
     2761 tattgaactg cccaaataca gggcaatgta tctggacagc cttttagagg aatcagaact
     2821 tatgggggtt gaaagggaca ctaattttat cagaatggtt caaaatataa aacagcccgg
     2881 aagtttggac ttcgaaattc cggaaaaact gaaaaatatc ctgagggact accagaaagt
     2941 tggatataag tggcttaaga cactgtcggc atatggactg tccgggatac tggccgatga
     3001 catgggattg ggtaaaaccc ttcaaataat atcctttgtt ctctcagaaa aggacagtgc
     3061 caaagggcct tcccttgtcg tcgcacccac ttcccttata tacaactggc aaaacgaagt
     3121 taaaaagttt gttccggagc ttaatgttct ggttgtatcc ggattgcctt cagagagaaa
     3181 agaactgctc ctgcaagccg gaaatgccga cctggtggta acgtcctacc ctttattgag
     3241 aagggacata gggttataca aggatataaa gttccaatac tgcttccttg atgaagcaca
     3301 gcatataaag aaccccaaca cccttaatgc caaaaccgtc aaacagatta aatccgaaat
     3361 gaattttgct ctgacgggta cacccattga aaactccata acagagctgt ggtctatttt
     3421 tgattatgta atgcccgggt atcttctttc tcacccgaaa tttgttaaaa aatttgaagt
     3481 cccgataatg aagcataatg ataagaatgc cctgaaggac ttaagtaagc acataagccc
     3541 ctttattttg agaaggctta aaaaagatgt tttaagcgag cttcccgata aaatcgaaag
     3601 taaattgacc tgtgaaatga ccccggaaca aaagaaaatt tatcttgcat acctgcaaaa
     3661 atccaagaaa gaaatatctt tggagcttga gtccagaggt tttgagaaaa gccatataaa
     3721 aatacttgct cttctgacaa gattgcggca gatatgctgc cacccttcaa tgtttatcga
     3781 aaacttcaag ggtgaaagcg gaaaaatgca gcttttggaa gaaatactat ccagtgcttt
     3841 cgacagcggg cacagggttc tgttgttttc ccagtttacc ggtatgctcg aaataataaa
     3901 gcaatccctt aacaaacagg gaatagaata tttttacctt gacggctcca caaaagccca
     3961 agaccgtttg gaaatggtgg attctttcaa cagcggttca aaaaaccttt tcctgatttc
     4021 tttaaaggct ggtgggacag gcttaaacct taccggagcg gatatggtaa tacactttga
     4081 cccatggtgg aatcctgccg ttgaggacca ggcttctgac cgtgcccata gaatagggca
     4141 aaaaaatgta gtccagataa tgaagctcat aacccgggga acaatagagg ataaaatatt
     4201 tgagctgcag caaaagaaaa aggaaatgat tgactcggtt ataaagccgg gggagaccct
     4261 tctttcaaag atgaccgaaa gcgagataag agagctgttt gagctataga aaatgaaggt
     4321 cttagaaaaa aagaggttag cctttataaa aaagactaac ccatgtaaag ggggtcaaaa
     4381 tgtattttgt gaggtcattt atattattga cccaatcccc ggttcattat acatcttttt
     4441 aaaaaaattt ttctgttgtt ttaattctgt ttattatctc agccactgaa gcttcaatat
     4501 ccatatcgga gcagttgact gtgatgtcgg catatttttc gtaaagaggt accctctcat
     4561 tatatagatc catcaaagtc ttatctttct cctttgcaat tccccggctg gaaatattgc
     4621 ttatcctctt ttctatttca tctaacctta gctttatata aacaacaaaa ccttcttttt
     4681 ttaaatgctt caaagcagca tcgctgtaaa taatacttcc tccggtggca atcacatgat
     4741 ttcttatatc catagaaaca gcaatttcct cctcaacgga aagaaacctc tttattccat
     4801 acttatcaat tatatcctgc agaaacatgt tatacttttg ctgtatcagc aaatccgtat
     4861 ctataaaggg ctttttcaag gctttcgcca aaagaacccc tattgtactt ttacccgctc
     4921 ccggcatacc tatcagcaca atatttgttt tttccataaa acaatccact cccaaaaaag
     4981 atatgcattc ctttatataa tagcacacat ctgtcaataa tatatactgc cataactttt
     5041 acaatgctca aacaaaggat aatttcaaaa atatcaagcc gagaagtagg taagcctaat
     5101 taatcctaat cggcagcaat cctatttcac aagcaaaaag accgggaggt aataaaacat
     5161 ggatcagaac aaaaatgatg taataaatgg cgaatcccta aacatagatg ccaccttgga
     5221 aaaactgtca agagagctta aagccgatga aactctaatc cttgacattg gaaacaatgt
     5281 tcttgggcaa acggatacaa tatatgacac attggtaacg aagggctatg acgtgcgaaa
     5341 atccttcaag aacggcagaa accagattct cgtaagcaaa aagcattaac tccgcataat
     5401 agcacagggg gacggttctt cc
//
LOCUS       DAOOJC010000121         5408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12143_length_5408_cov_4.018868, whole genome shotgun sequence.
ACCESSION   DAOOJC010000121 DAOOJC010000000
VERSION     DAOOJC010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12143_length_5408_cov_4.018868"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PLA01_04635"
     CDS             complement(<1..842)
                     /locus_tag="PLA01_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD00620.1"
                     /translation="MNIGKIVQISGSVIDVEFEHGHLPKKREALAVTVNGEVRIMEVA
                     QHVGRDTVRCIMLNESENLARNMEVTATGSGVRVPVGKCTLGRIFNVLGEVIDGGHPI
                     SETETRWEIHRKAPLFERQHPSTEILETGIKVIDLLEPYPKGGKIGLFGGAGVGKTIL
                     IQELIHNVAMKHGGYSIFVGIGERSREGNDLWREMHESGVADKTALVFGEMNESPGVR
                     MRVGLSALTMAEYFRDCEQKDVLLFIDNIYRFVQAGSEVSTLLGRMPSAVGYQPTLAE
                     EMGD"
     gene            complement(839..1759)
                     /gene="atpG"
                     /locus_tag="PLA01_04640"
     CDS             complement(839..1759)
                     /gene="atpG"
                     /locus_tag="PLA01_04640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012993200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPD00621.1"
                     /translation="MILNAKEIKNRIKSIHDTRKITNAMYMIASAKMKKAKKELDSTR
                     PYSDALHSEIKRIFRTVENLESRYFYPADGSEAPDGTYGVLVITADRGLVGSYNKGVI
                     KEAQKLISEHPDTKLYVVGEYGRRFFSRQQMPVVKNFLYSAQNPTMNRAREISAILLD
                     LFDKGELDKIFVVYSDLKNSLTTKVISTRLLPFHRMQSTSFTKNKIEAEVSAPFEFYP
                     SVEAVVDNVVKCWLSGFIYSALVGSFCSEQSARMMAMSSANKNAEKIVESLILQYNQM
                     RQAAITQEITEVTSGAKAQKIKRKKEMLQP"
     gene            complement(1760..3256)
                     /gene="atpA"
                     /locus_tag="PLA01_04645"
     CDS             complement(1760..3256)
                     /gene="atpA"
                     /locus_tag="PLA01_04645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPD00622.1"
                     /translation="MIPLMKDTIKGWTPKVLEQEVGTVLSVGDGIATISGLSNAAYQE
                     IVIFSSGVRGMVLELKRDVTECILFDDDQSVVEGSTVRRTGRTAGMPVGDGFKGRVID
                     ALGNPIDGKEPIEANGYRPIESPAPGIIDRKPVNKPLETGILAIDSLFPIGYGQRELI
                     IGDRETGKTTIALDTILNQKGKNIICIYVAIGQKASSVARTVNTLRQHGAMEYTIVVN
                     ASASDPAPLQYIAPYAGCALGEYFMNKGQHVLIVYDDLTKHATSYRSLSLLLERSPGR
                     EAYPGDVFYLHSRLLERSAQLSDEKGGGSMTALPIVETQAGDISAYIPTNIISITDGQ
                     IFLESGLFFSGQRPAVNIGLSVSRVGGDAQTRAMKSAVGTLRLELAQYQEMEVFMQFA
                     SDLDDATRRQLDYGHSLMQVLRQEKHRPMKQHEQIIILVAALNHVMQGIPPAEIKGFK
                     YGLISYIEQQAPYIIRKIETGEFNEEIKEDIVRLAREYPSQVRDGGDE"
     gene            complement(3558..4037)
                     /locus_tag="PLA01_04650"
     CDS             complement(3558..4037)
                     /locus_tag="PLA01_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HPD00623.1"
                     /translation="MNIPLNIDWQQILLHLLNFSILTLGLYLLLYNPIKNFMKERSGY
                     YNKLDSDAQDKLKQADDLKISYEKHMGDAEATVKERKARAVQEAQEEADKLLQNAKEQ
                     AEKLLADAREAAQQERARILKEAQQEVAQMVISATEKLLTQSASGTLDQFLDAVKKE"
     gene            complement(4050..4499)
                     /locus_tag="PLA01_04655"
     CDS             complement(4050..4499)
                     /locus_tag="PLA01_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPD00624.1"
                     /translation="MTISNLVNVINDGTKKISNLILPHTRNTGKRKINNSGFRRMALF
                     CLLVFLIVISVSTFVLAAPAEEQAVAGQAMDSISIKAISAAVAIGLGAAAAAIAMGIA
                     ISKSSESIARQPEAEGSIRTTLLLGLVFIETAIIYVLVIAIMIIFVL"
     gene            complement(4499..5263)
                     /locus_tag="PLA01_04660"
     CDS             complement(4499..5263)
                     /locus_tag="PLA01_04660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPD00625.1"
                     /translation="MTNKPDEKYETVKEMMRDAVLHDANKISLFGIMDVNPGLISAVV
                     ISVILLTAALFIRLFAIPRFKYIPGKFQLILEEIVGLFDRFAKTNSPHRNGFLGAYIF
                     GAGMYIFFGTLFELFGFQAITTMGHSTTLPAPLSDINGAIALGCLSYLIILFGGISAN
                     GLRGLGTSLKDFSMPLSMSFRLFGSMLSGVLVTELVYYYLRLSFVLPAIVGILFTLLH
                     AVIQSYVLVMLTASSYGEVSEPRKKEIYGHTPAVRR"
BASE COUNT         1510 a         1284 c          938 g         1676 t
ORIGIN      
        1 aaatcaccca tttcctctgc taatgtaggc tgatatccta ctgcggaagg catacgtcct
       61 aacaaggtag aaacctcgct gcctgcctgt acaaagcggt aaatgttatc aataaataac
      121 agcacgtctt tttgctcaca atctctaaaa tactccgcca tcgtcaatgc agaaagtcct
      181 accctcattc taacaccggg ggattcattc atttctccga atactagtgc ggttttgtct
      241 gctactccgc tttcgtgcat ttctctccaa agatcatttc cttcacggct tcgttctcca
      301 atgccgacaa aaatagaata gcctccatgt ttcatggcaa cgttgtggat gagttcctga
      361 attaaaattg tcttgccgac accagcacct ccaaacagac caatttttcc gccttttggg
      421 taaggttcca gtaagtcgat aactttaatt cccgtttcta aaatttcagt cgatggatgc
      481 tgcctttcaa acaaaggtgc ttttctatga atttcccatc gggtttctgt ttctgaaata
      541 ggatgaccgc cgtcaataac ctctcccagt acattaaata tccgtcccaa ggtgcatttt
      601 cctaccggaa cacgaacacc actaccggtt gccgttacct ccatgttgcg tgcaagattt
      661 tcgctttcat tgagcataat acatcggact gtatctctcc ccacgtgttg tgccacttcc
      721 ataatacgca cttcaccgtt gacagtaaca gcaagggctt ctcttttttt aggtaaatgc
      781 ccatgctcaa attctacatc tataactgaa ccggatattt gtacaatttt ccctatattc
      841 acggctgtaa catctccttt ttacgcttta ttttctgtgc ttttgcacca gatgtcactt
      901 ccgttatttc ttgtgtgata gccgcctggc gcatctgatt atattgaagg atgagagact
      961 caacaatctt ttccgcattt ttgttcgccg agctcattgc catcatgcgg gcactttgct
     1021 cactgcaaaa actaccaacc agagcactgt aaataaaacc ggataaccag cattttacca
     1081 cgttatctac aacagcctca accgaaggat aaaattcaaa aggtgcagag acctctgcct
     1141 caatcttgtt ctttgtaaaa gatgtagact gcattcggtg aaaaggtaaa agtcgggtgg
     1201 atatcacttt tgtagtcaga ctgtttttga gatcactgta aactacaaaa attttgtcca
     1261 gttctccttt atcaaacaaa tccaaaagta tagcactaat ttctctggcc ctgttcattg
     1321 ttggattttg agccgaatat aaaaaatttt tcacaacagg catttgctgg cgtgaaaaga
     1381 atcgccgtcc atattcccca acaacataaa gtttggtgtc cggatgttca gatataagct
     1441 tttgggcttc tttgattaca cctttgttgt acgagcctac taatcccctg tctgcagtaa
     1501 ttaccaaaac accgtaagtt ccgtcggggg cttcacttcc atctgctggg tagaaatatc
     1561 tgctttccaa attctccaca gttcggaata ttcgttttat ttcactgtgc aatgcatcag
     1621 aatacggtct tgtagagtct aattcctttt ttgctttttt catcttagca gaagcaatca
     1681 tgtacatggc gtttgtaatt ttccttgtgt catgaatact tttaattcgg tttttgattt
     1741 ctttggcatt taaaatcatc tattcatcac cgccatcacg tacctgactt ggatattccc
     1801 ttgcaagcct cacaatatct tctttgattt cctcgttaaa ttctccggtt tcaattttcc
     1861 tgattatata cggtgcctgt tgttcaatat aactgatcaa tccatactta aatcccttga
     1921 tctctgcggg aggaataccc tgcattacat ggtttaatgc tgctaccagg ataataatct
     1981 gttcatgctg tttcattggc cgatgttttt cttgtcgcag cacctgcata agactatgac
     2041 cgtagtccag ttgccgtctt gtagcgtcat ccaaatcgct ggcaaactgc ataaagactt
     2101 ccatctcttg gtactgtgcc aactccaggc ggagggttcc tacagccgat ttcattgctc
     2161 ttgtctgtgc atcgccccct acacgggaaa cggaaagacc aatgtttaca gccggacgct
     2221 gaccggaaaa aaacaaaccg ctttccaaaa atatttgtcc gtctgtaata gagataatgt
     2281 tcgttggtat gtaagccgaa atatcacccg cctgtgtttc cacaatagga agtgcagtca
     2341 tactgcctcc gcccttttca tcggaaagct gtgccgaacg ctctaataaa cgggaatgaa
     2401 gataaaaaac atccccggga taggcttcac gccctggaga acgttcgagc aaaagactta
     2461 aagaacggta tgaagtagca tgtttcgtca aatcatcata aacaatcagt acatgctggc
     2521 ctttattcat aaagtattct cccaatgcac agcccgcata aggtgcaata tactgaaggg
     2581 gtgccggatc gcttgcagat gcattaacca caattgtata ttccattgca ccatgttgac
     2641 gaagggtatt caccgttcgt gcaacggaac ttgccttttg accgattgcc acataaatgc
     2701 aaataatatt tttgcccttt tgattaagta tagtatccag tgcaattgtg gtctttcctg
     2761 tctcacggtc accgataata agttcacgct gcccataacc aatgggaaac aaagagtcaa
     2821 ttgccaaaat tcctgtttcc aagggtttat taacaggttt gcggtcaata attccgggag
     2881 caggtgattc aattggacga tagccatttg cttcgattgg ttccttccca tcgataggat
     2941 tgcctaatgc atcaattaca cgtcccttaa agccatcacc cactggcatt cctgcggtcc
     3001 ttccagtcct tctcaccgtg ctgccttcaa caacgctttg gtcatcatca aatagtatac
     3061 actcagttac gtcacgtttt agttccagaa ccattcctcg aacaccggag gaaaaaatca
     3121 caatctcttg ataagcagca ttgctaagac cgcttatagt agcaatacca tcaccaaccg
     3181 acaataccgt tcctacttcc tgctccagta ctttcggggt ccagcctttt atagtatctt
     3241 tcattagggg aataatattg ccgttttcga ctgagagttg agttattttc tctttcagct
     3301 gctgtaacct tcctactaaa ctccaatcat atatagattc tagtttccaa ttgccagcag
     3361 tgctatcacc gtctaccgtt ccaatttcca ggcgaaagcc ttgagctaac tccccatcct
     3421 ctttccacaa aagcgtaata tctttattat atttattttt taaaaagctt atcagctgtt
     3481 gtctttgtgt atcgcttggg gcctcagcac tatacagtat cgcctcaacc cttatgtttt
     3541 tattgtctat cgcaatttca ctcctttttt acagcgtcta gaaattggtc aagcgttccc
     3601 gatgctgatt gggtaagtaa tttttccgta gcagaaatca ccatttgggc tacctcctgc
     3661 tgagcttctt ttaaaattct agcacgttcc tgctgtgcgg cttcccgggc atctgcaagt
     3721 aatttttcag cctgctcttt agcattttgc aatagtttgt cagcctcttc ctgtgcttcc
     3781 tgtacagcac gtgcctttct ttctttaacg gttgcttcgg catcccccat gtgcttctca
     3841 taggatatct tcaaatcgtc ggcttgcttt aatttatctt gtgcatcgct gtccagtttg
     3901 ttataatacc ctgatctttc tttcataaag ttcttaattg ggttatacag caacaaatac
     3961 aatcctaaag ttagaatcga aaagtttagt agatgtaaaa gtatctgctg ccaatcaatg
     4021 tttaaaggga tattcatcta tttcactccc tacagtacaa atataatcat aatggcaata
     4081 actaaaacat aaataatagc tgtttctatg aatacaagtc ctaaaagtaa agtagttcta
     4141 atacttcctt ctgcctcagg ctgacgtgca atactttctg atgattttga aattgcaatt
     4201 cccatagcta ttgcagcagc tgcagcacca agaccgatgg ccacagcagc agaaattgct
     4261 ttaatactta tagagtccat tgcttgcccg gccactgctt gttcttcagc aggagcagca
     4321 agtacaaaag tagaaaccga aattacaatc aaaaacacca aaagacagaa taaagccatt
     4381 cttcgaaaac ctgaattatt aattttcctt ttaccagtgt ttctggtatg gggcaatatt
     4441 aaatttgaaa ttttctttgt tccatcattt ataacattca caagattact aattgtcatt
     4501 acctcctaac agcaggcgta tgcccgtata tttctttttt tcgcggttcg gatacctctc
     4561 cgtaagaaga tgccgtaagc ataactaata catatgattg gattacagca tgcagcaaag
     4621 tgaataatat acctactata gccggcaaaa caaagctaag ccgcaaataa taataaacca
     4681 attcggttac caacacaccg ctgagcattg atccaaacag acgaaagctc atagacagag
     4741 gcatcgaaaa atctttcaat gaagttccca gccctctaag accatttgcc gatatacccc
     4801 cgaacaaaat aataagatac gataggcacc ctaaggcaat tgccccattt atgtccgata
     4861 acggtgccgg gagagtagtg gagtgaccca ttgtggttat tgcctgaaag ccaaacagct
     4921 caaacaaggt tccaaaaaag atatacatgc ctgcaccaaa aatataggca cccaaaaacc
     4981 cattacgatg cgggctgttt gttttggcaa atctatcaaa caaaccaact atctcttcca
     5041 atatcagttg gaactttccg ggaatatatt tgaatctggg aatggcaaac aatcggataa
     5101 acagtgctgc ggttaacaag attacggaaa taacaactgc tgatataagg cccggattaa
     5161 catccattat tccaaaaagg cttattttat ttgcatcatg cagtactgca tctcgcatca
     5221 tttctttaac agtttcatac ttttcatccg gtttgtttgt caatataact cccacaaaca
     5281 agactaatat aacacataac catccggcaa taatttttgc ctttttatta ttaatctgag
     5341 gtaacctccc ttcttttaaa actccttttc attttccgta aaaggacttg taaaaactat
     5401 cataattc
//
LOCUS       DAOOJC010000122         5378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12217_length_5378_cov_3.186925, whole genome shotgun sequence.
ACCESSION   DAOOJC010000122 DAOOJC010000000
VERSION     DAOOJC010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12217_length_5378_cov_3.186925"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..237)
                     /locus_tag="PLA01_04665"
     CDS             complement(49..237)
                     /locus_tag="PLA01_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00626.1"
                     /translation="MEMLFLKVLNMSITAGYVILAVLIVRLLLIRILYIKTVKSHWTT
                     KPKMVLRRIVYTYINKIN"
     gene            complement(240..602)
                     /locus_tag="PLA01_04670"
     CDS             complement(240..602)
                     /locus_tag="PLA01_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HPD00627.1"
                     /translation="MGDLKLCESDYRFMQVVWENEPVASGKLVTLCAGKLGWKKSTTY
                     TVLRKMCEKGFLKNEDTIVTSVIPMEKVQAYESEYFVERTFDGSLPKFLASFLGGKTL
                     SEKDVEEIKRLIDAHKEG"
     gene            complement(779..2107)
                     /locus_tag="PLA01_04675"
     CDS             complement(779..2107)
                     /locus_tag="PLA01_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD00628.1"
                     /translation="MKRKINSQLIMITAIAIISMLIFLTAVFNELFKRQVIEDLKGYA
                     DLLEEFYDFNESGLDYPDLKVKGVRITLVSSEGVVVYDTHEDTVSMSNHAKRPEVLRA
                     LEKGEGESVRRSETLMHNQFYYAKLLDNGKVLRVSKSAGNIWAVIRRALPLTIVIIIL
                     LFIMCAVLSDVLTKRLVEPIEVMAAHLNDNDMEPAYEELKPFADTIRKQHNEIMKAAN
                     IRQEFSANVSHELKTPLTSILGYAQLIENGMAKKDETLRFAGEIHRNANRLLTLINDI
                     IRLSELDGGAGEAEYADIDLFDIARACVDNLKATSREHKVTIKLRGTHQTIYGNRIMI
                     EELVYNLCENAIKYNVKYGEVYVTVNMKNGVVFLSVKDTGIGIPHEHQERIFERFYRV
                     DKSRSKLLGGTGLGLAIVKHIVMQHGARIELKSEEGKGSEFIVKFSSKKE"
     gene            complement(2091..2768)
                     /locus_tag="PLA01_04680"
     CDS             complement(2091..2768)
                     /locus_tag="PLA01_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPD00629.1"
                     /translation="MSLIYVLEDDENIREIEIYALKNSGYQVKGYTTVKDFYKALEDM
                     IPSLVILDIMLPDEDGIEVLKKLRSKSETKKVPVIMLTARTTEMDKVKGLENGADDYI
                     TKPFGVMEFVARVKAVLRRSEASYGMFLRLGAISVDNDKRVAYIKDKPCNLTYKEFEL
                     LKLFVCNAGIVLTRENIMNRIWETDYVGETRTVDMHVKTLRKKLGKFGDMIKTVRNVG
                     YKIEEED"
     gene            complement(2900..4681)
                     /locus_tag="PLA01_04685"
     CDS             complement(2900..4681)
                     /locus_tag="PLA01_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HPD00630.1"
                     /translation="MDIFGVLTLIGGLALFLYGMHVLGDGLTNLSGGKLESILAKLTS
                     NRLKGVLLGLGVTAVIQSSSATTVMVVGFVNSGIMKLSQAVGVIMGANVGTTVTPWIL
                     SLTGIESENVLIKMLKPSSFSPILAAIGVILLFSAKSDKKKQLGNILLGFSVLMFGME
                     MMSDAVSPLKDIPEFTSLLTMFSNPVLGMIVGASLTAIIQSSSASIGILQALCTTGVV
                     SYSTAIPVIMGQNVGTCITAILSSIGANKNAKRASGIHLYFNLIGIVLFMSLFYSINF
                     LSPFAFLNDSANQMGIAVIHTTFNVFATLVLFPFSSYLIKLSELTIRDDKKEDSGEKT
                     QDMPEALKALDARFLEMPGFAVTQCRRAVLEMAGITKEALERAIGLIWSYDEGAYKEV
                     EDKEKLVDEYEDIIGSYLVKLSTRHLMEEDSKGMSFLLHSINDLERISDHAVSIAKSA
                     KEITEKGKDFSKKALQELEVISQAVMQICSSTVDILDKEDNDIANSIQPLNEVIELIQ
                     IEIKERHIKRLKNGKCTIKKGFILTDIITSFQRVAAHCSNIALSMIQINQESLETHGY
                     AASIPKGEGSTYYNEFNKYKNKYVLSD"
     gene            complement(4870..>5378)
                     /locus_tag="PLA01_04690"
     CDS             complement(4870..>5378)
                     /locus_tag="PLA01_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030248 - cellulose binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose binding domain-containing protein"
                     /protein_id="HPD00631.1"
                     /translation="SGGLGGQNSTKNKVQSSKGTVSIQYANGNAGATSNSINPKFKIT
                     NNDTKPIKLSDVKIRYYYKIDGGNTQNFWCDWSSAGNSNVTGNFIKLSSPKTGADTYL
                     EVGFTDGAGTLEPGQSVEVQTRFSKDDWSNYNQTSHYSFNSSASDYIDWDKITLYVSG
                     KLVYGAEP"
BASE COUNT         1530 a         1249 c          849 g         1750 t
ORIGIN      
        1 taaattatac tatatagttt atgtcccaaa aagccaaata cattaatatt agttaatttt
       61 gttaatgtat gtatatacta tcctgcggag taccattttg ggcttggttg tccaatgtga
      121 tttcaccgtt tttatataca atatccgaat taaaagtaac cgtacgataa gcaccgcaag
      181 gatgacatag ccggctgtaa tgctcatgtt taataccttt aaaaacagca tctccatgtt
      241 catccctcct tatgggcatc aatcaagcgt tttatctctt cgacatcctt ttccgaaagg
      301 gttttaccgc caagaaacga tgcaagaaac tttggaagtg aaccgtcaaa agttctttca
      361 acaaagtact cgctctcgta cgcctgaacc ttttccatag gtatgacaga ggttacaatg
      421 gtatcctcat tttttaaaaa tcccttctcg cacatctttc gaagtacagt atatgtggtc
      481 gattttttcc aaccaagctt cccggcacac aaggttacaa gcttccccga tgccacaggc
      541 tcattctccc ataccacctg cataaaccga tagtcgcttt cgcaaagctt cagatctccc
      601 atccttatcc ctcccctgca gtctataata ataaaccaca tttttagttt atcactatag
      661 accaaattta tcaatagaaa accttctctt ttgggtagaa aatgtcttta gcattaattt
      721 ttcaacatga caggaagaca ttagattatc agagatttgt gggaaaatat ctttattatt
      781 attctttctt ggagctgaat ttaactataa actcactgcc ttttccttct tcgcttttaa
      841 gttcgatcct tgcaccatgc tgcattacaa tatgtttaac tattgcaagg ccaagtcctg
      901 tgccgccaag caattttgac ctgcttttat ccacacggta aaatctctca aaaatacgtt
      961 cctgatgttc atggggtatt ccgattcccg tgtcttttac agacaaaaag accaccccgt
     1021 ttttcatatt taccgtaaca taaacctctc catattttac attgtatttt atggcgtttt
     1081 cgcaaagatt ataaaccaat tcctctatca ttatccgatt tccatatata gtctgatgtg
     1141 tccccctaag cttaattgta accttgtgtt ctctgcttgt tgctttcaaa ttatcaacac
     1201 atgctcgggc aatgtcaaac aaatcaatat cggcatattc ggcttcccct gcccccccat
     1261 ccagctcaga aagtcttatg atatcgttga ttaatgtaag gaggcggttg gcattcctgt
     1321 gaatctctcc ggcaaacctg agcgtttcat ccttctttgc cattccgttt tcaatgagtt
     1381 gtgcataacc cagaattgaa gtcaacggtg ttttcaattc atgggatacg ttggctgaaa
     1441 actcctgtct tatattcgca gccttcataa tttcattgtg ctgttttctt atcgtgtctg
     1501 caaaaggttt taattcttca taggcaggtt ccatatcatt atcgtttaaa tgtgccgcca
     1561 taacttcaat gggttcaaca agtcttttcg taagaacatc cgataaaaca gcacacataa
     1621 taaacaatag gattatgatt actattgtga gaggaagagc ccttcttata acagcccata
     1681 tgtttccggc cgatttggat accctgagta ccttgccgtt gtcgagcagt tttgcataat
     1741 aaaactgatt atgcattaaa gtctctgatc ttcttacgga ttctccttcg cccttttcca
     1801 gagccctcaa aacttccggt cttttggcgt gattggacat tgaaacggtg tcttcatgag
     1861 tgtcatacac aactacgcct tcagaactca ccagtgttat tctaacgccc tttactttca
     1921 aatcaggata atcaagacca ctttcattaa aatcatagaa ttcttcaagc aagtccgcgt
     1981 atccttttaa atcttcgata acctgtcgct taaaaagttc attaaatact gcggtcaaaa
     2041 aaatcagcat tgaaataata gctattgcag ttatcataat aagctggcta ttaatcttcc
     2101 tcttcaattt tgtaccccac gtttctaacc gtttttatca tgtcgccaaa ctttccaagc
     2161 ttctttctta atgtctttac atgcatatca acagtcctcg tctcaccgac gtaatctgtt
     2221 tcccaaatcc tgttcatgat attttccctc gtaagtacga tacccgcatt gcacacaaaa
     2281 agcttaagca attcaaattc tttgtatgta aggttacaag gcttatcctt tatatatgcc
     2341 actctcttgt cattatccac agaaattgca ccaagtctta aaaacatacc gtatgatgct
     2401 tcactacgcc ttaaaactgc ctttactctg gcaacaaact ccatcacacc aaagggcttt
     2461 gtaatataat catcagcacc gttttccagg cccttaacct tatccatctc tgttgtcctg
     2521 gctgtaagca taatcaccgg tacttttttc gtttctgact ttgaacgaag tttctttaac
     2581 acttcaatcc cgtcctcatc aggcagcata atatcaagaa taaccaggga cggtatcata
     2641 tcctccagtg ccttgtaaaa atctttcact gtggtatatc ccttcacttg ataaccgctg
     2701 tttttaaggg catagatttc tatttcccgt atattctcat cgtcctccaa aacatatatc
     2761 aacgacatag cctttcccca ttcaaataaa tatcatctat attataccaa atcggatacg
     2821 ttaaatatat gcgaagaaaa ttgttccagc cttaaatagt atgcaccctt tacatgcctt
     2881 cttataataa actgtgggat caatctgaaa gaacatattt atttttgtac ttgttgaatt
     2941 cattgtaata cgtgctgccc tcccctttag ggattgatgc agcataccca tgggtttcaa
     3001 ggctttcctg atttatctgt atcatggaaa gtgctatatt gctgcagtga gcagccaccc
     3061 tttgaaaact tgtgataata tctgtaagga taaatccctt tttaatagta catttgccgt
     3121 ttttgagccg ctttatatgc ctttctttaa tctctatctg aataagttct attacttcat
     3181 tcaacggctg tatactattg gcaatgtcat tatcctcttt atcaagaata tcaactgtac
     3241 tgctgcatat ttgcataacg gcctgactta tgacctccag ttcttgcagg gctttctttg
     3301 aaaaatcctt gcctttttcg gttatctcct ttgctgattt ggctatcgat acggcatggt
     3361 ccgaaatacg ctctaagtca tttatggagt gcaaaagaaa tgacatgccc ttagaatctt
     3421 cttccataag atgccttgta ctgagcttaa ccaaatagct tccgataata tcctcatatt
     3481 catcaaccaa tttttccttg tcctcaacct ctttataagc accttcatca taactccata
     3541 taagaccgat tgccctttca agggcttcct ttgttatccc cgccatttcc agtaccgctc
     3601 ttctgcattg cgtgactgca aaccccggca tctccaaaaa tcttgcgtca agagctttga
     3661 gagcttcagg catatcctgt gtcttctccc ctgaatcttc ttttttatca tctcgtattg
     3721 tcagttcaga taactttata agatatgaac tgaacgggaa taacaccaat gtagcgaaaa
     3781 cattaaatgt agtatgaatt acagcaattc ccatttgatt tgcactgtcg tttaagaaag
     3841 caaagggtga caaaaagttg atggaataaa acaaagacat aaacaagacg attcctatca
     3901 agttaaaata caaatgaata ccggaagccc tcttggcatt cttattcgca cctattgatg
     3961 aaaggatcgc tgtaatacat gtaccaacat tctgtcccat aattaccggt attgcagttg
     4021 aataacttac cactccagtg gtacaaagag cctgcaaaat accgatagaa gccgacgaag
     4081 actgaattat ggccgtaaga ctcgctccaa ctatcatacc caacacagga tttgagaaca
     4141 ttgtcagcag tgatgtaaac tccggtatgt cttttagagg tgaaactgca tccgacatca
     4201 tctccattcc aaacataaga acggaaaaac ccaatagaat atttccgagt tgctttttct
     4261 tatcgctttt agctgaaaac aacagtatta ctcctattgc agccaatatc ggagaaaagg
     4321 aagaaggctt taacatctta attaaaacat tttcactttc aattcctgtg aggctaagta
     4381 tccatggtgt tacagtagtt ccaacattag cccccataat gactccaacc gcttgagaaa
     4441 gtttcatgat gcctgagttt acaaatccaa ccaccattac cgtggtcgct gatgatgatt
     4501 gaataactgc cgtaacccca agtcccaaca acacgccttt aagccggttt gatgtaagct
     4561 ttgcgagaat actctcaagc tttccccctg acagattggt aagcccgtcc cccaacacat
     4621 gcattccgta taggaaaaga gcaagtccac ctatcaacgt aaggacacca aaaatatcca
     4681 taaatccact ccccttgtaa cacttataca tattatataa aataaataaa ttgcattgat
     4741 aacatcgctc tgttacatta tcataataac acacccatgt aaatttcata ggacatctgc
     4801 gtaaaatata tgtaaaattt tctgttcggc ttttcttctt tccatttcaa tgttgatact
     4861 caactttttt caaggttctg caccataaac aagctttccg gaaacgtaca atgtaatttt
     4921 gtcccaatcg atatagtccg atgccgatga attaaaagaa taatggcttg tctggttata
     4981 atttgaccaa tcatccttgg aaaacctcgt ctgcacttca acgctttgtc ccggttcgag
     5041 ggtccccgct ccatcggtaa atccaacctc aagataggta tctgcacccg ttttgggaga
     5101 tgagagcttg ataaagttcc cggtaacatt cgaatttccc gcactgctcc aatcgcacca
     5161 gaaattttgt gtattgcctc catcaatttt atagtagtat ctgattttta catccgatag
     5221 ttttataggc tttgtatcgt tgtttgttat tttgaatttt gggttgatgc tgtttgatgt
     5281 agccccggca tttccattgg catactgtat cgaaaccgtg cctttactgc tctgtacttt
     5341 attctttgta ctgttttgac cgccaaggcc gccggatt
//
LOCUS       DAOOJC010000123         5362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12269_length_5362_cov_3.524402, whole genome shotgun sequence.
ACCESSION   DAOOJC010000123 DAOOJC010000000
VERSION     DAOOJC010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12269_length_5362_cov_3.524402"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..2535)
                     /locus_tag="PLA01_04695"
     CDS             complement(121..2535)
                     /locus_tag="PLA01_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPD00632.1"
                     /translation="MDKKDLFQLLKENILILDGATGTELQKRGMPQGVCPEMWAIENP
                     DVIVDTQKEYIQAGSNVVYTCTLGGSRIKLSEFGLGDRTVEINRKQALLSRQAVGENG
                     LVAGDIGPMGQFVRPFGDLPFEDAVNIFKEQVKGLLEGGVDFFVIETMMDIQEARAAL
                     IAVKESCDLPVCVSMTYDQGGRTLTGTDPVTALMILQNLGADVVGCNCSTGPGDMIKI
                     IRAMKPYAKVPLLAKPNAGLPKLVNGKTQFDMDVEEFGSYVKEFVEAGVNLLGGCCGT
                     SPLYIEQIRKNISGLKPIAPMPKKICAVASARKTVFVDADEPVVVVGERINPSGKKML
                     QEELRQGMTSLIKQFAREQVENGASVLDVNVGMPGIDEKKTMLRVVEELSVMTDAPLC
                     LDSSSPEVLEAALRVYPGRALINSISAERVKLEKLLPITAKYGAMFILLPLSDEGVPE
                     TSQERYEIVERVYNQAAGFGYTKNDFVVDGLVMTVSADQKAAIETLKVIDWCSNAFGC
                     GTIVGLSNVSFGMPERKWINSAFLAMAIGRGLTMAIMNPSGDTLMNIKMASDVLKIKD
                     KNSKKYIEYFAGQKEQKPAAHQKEETVSTVDRIYGAVVKGDRESVVQYIDIALKEGAE
                     PSHIVDKYLIPAITHVGDLYDKKEYFLPQLIQSAETMKTAFGIIEPLLTKDADGDGKK
                     KVRVVLATVKGDIHDIGKNIVGLMLRNYGFEVYDLGKDVAAGEIISKAKETKASIIGL
                     SALMTTTMLEMKEVVDLAKKEGLDAKIMIGGAVVNAEYAKEIGADGYSEDAYAAVKLA
                     GKLSQI"
     gene            complement(2528..3211)
                     /locus_tag="PLA01_04700"
     CDS             complement(2528..3211)
                     /locus_tag="PLA01_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HPD00633.1"
                     /translation="MEILIMMSGDFMALEQYGKLKYFEHIPAVPDNGMILTRLGYRKT
                     KTVLDEDYKKKLKANIDMGLMLCNTKGVFSRLSIIKRDDESVELENGAVLKSKSLAKL
                     LQNSMEVVLMAATVGKDIVDRIHDEVENNDAAFGLVLDAVASETADAALDWMVDFINK
                     LIRREGKKLTGMRYSPGYGDLPLTNQKIIFDILDLGRLGLRITERFMLIPEKSVIAIA
                     GVEGVESNG"
     gene            complement(3359..3940)
                     /locus_tag="PLA01_04705"
     CDS             complement(3359..3940)
                     /locus_tag="PLA01_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00634.1"
                     /translation="MGAVVELTMYYKDIIPFSRIINRIEDYGYRVILNEIEVIDDWSY
                     SNRQVLKFNEDTIDFDELMSDNKIILAHFTANEFKCGYYIFKADTDIYEMCIWLNTDG
                     ISFLDNDVIDERNKFVYDKIKNELIANIDSNKLLLIGIGVETLIKYDKSLQNIVDNSK
                     NVLMWIIPKDKKIRIHHNYKIEDKRDLLIYSLE"
     gene            complement(3975..4553)
                     /locus_tag="PLA01_04710"
     CDS             complement(3975..4553)
                     /locus_tag="PLA01_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00635.1"
                     /translation="MINIDNIGNLKKCRIWLEEFSVAKHNAVEVLSSALKASDSIVKN
                     DQSVAVELFAAPRYYALLGVEYIYKDTGKLEIYINTSEDSEKISDSLALPSDNVQSGI
                     PKEYAQTVLNTTIKVFRNLYSIPSGILTFSVGAYGNFGSNQVIFAKATGILIGLLVGK
                     LSNMELDELKNIVRNELNKPLTDFYYDDFKDK"
     gene            complement(4558..4836)
                     /locus_tag="PLA01_04715"
     CDS             complement(4558..4836)
                     /locus_tag="PLA01_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00636.1"
                     /translation="MKNCIVIEITNNDNEKIVKQLDLNDWYDQGIVEIDDEKYRKEHK
                     IVNIKGIQYDQNGRVEVSWKTYYDNLGRLIKTEEYDENGNLIKSEEIS"
     gene            complement(4868..>5362)
                     /locus_tag="PLA01_04720"
     CDS             complement(4868..>5362)
                     /locus_tag="PLA01_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00637.1"
                     /translation="IFKQGIFINDWKDNVYFRVKKGRNDGDPDDALQNYLMLPIYSPR
                     LITALDINEIRGIQYLPIQVIKFNGVCLDGFCIANMLNFIEAFDEEKSVFDRFSEDFP
                     NPNVRGKIAGVRKFVLKKKNLEGYDIIRLKEYKQRFFVSDKFKNIFQSNKFTGYSFRE
                     VELV"
BASE COUNT         1515 a         1264 c          785 g         1798 t
ORIGIN      
        1 tttttctttt gaaatgaaat gatgtagaaa ccacaacagc atcaagatgt tgttgtggtt
       61 tattatatca cacactatat attgtgtcaa taagaaataa ttattcagga aatattatat
      121 tcagatttgc gaaagcttac ctgcaagctt taccgcagca taggcatcct ccgaatatcc
      181 atcggcacct atttcttttg catactcggc attaaccact gctccgccga tcattatttt
      241 tgcatcaaga ccttcttttt tggcaagatc tacaacttcc ttcatctcaa gcattgttgt
      301 tgtcataagt gcagaaagtc caattattga ggcttttgtt tcctttgcct ttgatattat
      361 ttccccggca gctacatcct tgcccaggtc atacacctca aaaccatagt ttctgagcat
      421 cagccccact atattctttc ctatgtcgtg tatatccccc ttaaccgtag ccagaactac
      481 ccgaaccttt tttttgccat ccccatctgc atcttttgta agaaggggtt ctattattcc
      541 aaaggccgtt ttcatggttt cggcactttg aatcaattgg ggcagaaaat actccttttt
      601 gtcataaaga tcccccacat gagtaattgc aggaatcaaa tatttatcaa caatatggga
      661 aggctcggct ccttctttca atgcaatatc gatatattgc accacactct cccggtcgcc
      721 cttaacaacc gcaccataga ttctatcaac ggtgctgacc gtttcttcct tttggtgagc
      781 agcaggcttt tgttcctttt gacctgcaaa atactctatg tattttttgc tgtttttgtc
      841 ctttatcttt aaaacgtccg atgccatctt tatattcatc agagtatcac ccgacggatt
      901 cataattgcc atagtaaggc cccgtccgat ggccatggca agaaaggccg aattgatcca
      961 tttcctttcg ggcataccga aggacacatt tgaaagtccc actatcgttc cgcaaccgaa
     1021 agcattgctg caccagtcta tgacctttaa ggtttcaatg gctgccttct ggtcagcaga
     1081 tacggtcata acaagtccgt ccacaacaaa atcgtttttt gtataaccga atcctgcggc
     1141 ttggttatac accctctcga ctatctcata cctttcctgt gatgtctcag gaactccctc
     1201 atcgctcaaa ggcagaagaa taaacattgc tccgtacttt gcagttatcg gcagcagctt
     1261 ttccagctta accctttcgg cagatatgga atttataagt gcacgtccgg gatacaccct
     1321 cagtgccgcc tccaaaacct ctggtgagga cgaatcaagg caaagaggtg catcggtcat
     1381 gactgacagt tcctccacca ccctaagcat ggttttcttc tcatctattc ccggcattcc
     1441 aacgtttaca tccagcacgc tggccccatt ttccacctgt tcccgggcaa attgctttat
     1501 caaagacgtc atgccctgcc ttaattcctc ttgaagcatt tttttacctg atgggtttat
     1561 cctttctccc actaccacaa ccggttcatc tgcatctaca aaaaccgttt tgcgtgcaga
     1621 agcaacggca catatttttt tcggcattgg agctattggc tttaatccgc taatattctt
     1681 tcgaatctgt tcaatgtaca ggggcgacgt accgcagcat ccacccaaaa gatttactcc
     1741 ggcttcaaca aactctttaa catagcttcc gaattcttcg acatccatgt caaactgtgt
     1801 cttaccgttg accagcttgg gaagtccggc attgggcttt gctaaaagcg gaacctttgc
     1861 ataaggcttc atagcccgta ttatttttat catatcccca ggtccggtgg aacagttgca
     1921 tcccactaca tctgcaccaa ggttttgcag aatcatcagt gccgtcacag gatcggtacc
     1981 tgtaagggtt ctcccacctt gatcataagt catactaacg cataccggaa ggtcacagct
     2041 ctctttaacg gcaatcaatg ctgcccttgc ctcctgaata tccatcattg tctctattac
     2101 aaaaaagtca acaccgcctt ctaaaaggcc ttttacctgt tccttaaaaa tattgaccgc
     2161 atcttcaaag ggcaagtctc caaagggtct gacaaattga cccataggcc ctatatcgcc
     2221 ggcaacaagt ccattttctc cgacagcctg ccttgaaagc agtgcctgct ttctgtttat
     2281 ttcgaccgtc ctgtcaccca atccgaattc ggaaagtttt attctgctgc cccccaatgt
     2341 gcatgtatat acaacattag accctgcttg tatgtattct ttctgtgtat ccacgatcac
     2401 atctggattt tcaattgccc acatctccgg acaaactccc tggggcatcc ccctcttttg
     2461 aagctctgtc cctgtagcac cgtcaagtat caatatattt tcttttaata actgaaataa
     2521 atccttttta tccattagat tcaacccctt ccacacctgc aatggctata acagacttct
     2581 ccggaatcag cataaacctt tctgtaatcc taagtcccag cctgcctaga tccaatatgt
     2641 caaaaataat cttctggttt gtaagaggaa ggtctccata cccgggactg tatctcatcc
     2701 cggtaagttt tttcccttct cttctaatca atttatttat aaaatcaacc atccaatcaa
     2761 gggcagcatc cgcagtttcc gatgcaacgg catccaaaac aagcccaaat gcggcatcat
     2821 tgttttccac ctcatcatga atcctgtcca caatatcctt tcctaccgta gctgccatca
     2881 aaaccacttc catgctgttt tgaagaagct ttgcaaggct tttgctctta agaacggcac
     2941 cattttccag ttcaaccgac tcatcatccc gctttattat gctaagcctt gaaaaaaccc
     3001 cttttgtgtt gcacagcatc aatcccatat ctatgttggc ttttagtttc tttttataat
     3061 cctcatccaa aaccgttttg gttttcctgt agcccaatct tgtgagaatc ataccgttat
     3121 cgggaactgc cggtatgtgt tcaaaatatt ttaatttgcc atactgttcc aaagccataa
     3181 aatcaccgct catcattatc agtatttcca ttatatatga aagcaaaaaa aaatcaaccg
     3241 attaattaag tccataattg aaaagatgca atatgtaaaa tggaaattaa aatgaacaga
     3301 gcttttgtgt tccatgcatt tcttgcagct tattcactac attatccatt attctcatct
     3361 attctaggga atatatgagt aagtcccttt tgtcttctat tttgtaatta tgatgtatcc
     3421 ttattttttt atccttagga ataatccaca tcagtacatt tttgctgttg tcgactatgt
     3481 tttgtaatga cttgtcatac tttatcaggg tttctacacc aattccaatt aataatagct
     3541 tattgctgtc gatattggca attaactcat ttttaatctt gtcataaaca aatttattcc
     3601 tctcgtctat aacatcatta tctaaaaatg atattccatc cgtatttaac caaatacaca
     3661 tctcataaat atctgtgtct gctttgaaaa tataatagcc gcatttaaac tcatttgctg
     3721 taaaatgagc taatattatt ttgttatcag acatcagttc atcaaaatca atcgtatctt
     3781 cattaaattt taatacttga cggttagaat aggaccaatc atcaattacc tcaatttcat
     3841 ttaaaattac ccgatagcca taatcttcta ttcggttaat aattctgcta aaagggatga
     3901 tatctttata atacattgtt aattccacga cagctcccat ttttcaatct ctcctttttg
     3961 tgcagcattt cgtactattt atctttaaaa tcatcatagt aaaaatcagt caatggctta
     4021 tttaactcat ttctaacaat gtttttcaat tcatcaagtt ccatattact gagctttcca
     4081 acaagtaatc caatcaaaat acctgtggct tttgcaaaaa tgacttgatt agagccgaaa
     4141 ttgccataag caccaacact aaaggttaaa atcccggaag gtatactgta taaatttcta
     4201 aatactttta ttgttgtatt taaaacggtt tgggcatatt cctttggaat tcctgattga
     4261 acattgtcgg aaggaagtgc taatgaatca ctgatctttt cggaatcttc agaagtatta
     4321 atatagatct ccaatttgcc agtgtcttta tatatgtatt caacacccaa aagagcataa
     4381 tatcttggag cagcaaacag ctctactgca acagattggt catttttaac aatactatct
     4441 gaagctttca atgcagagct caatacttca acagcattat gttttgctac cgaaaactcc
     4501 tcaagccaaa ttctacattt ctttaaattt cctatgttat caatgtttat catcgtctta
     4561 acttatttcc tcagatttaa ttaaattacc gttctcatca tactcttcgg tttttattaa
     4621 acgtccaaga ttatcatagt atgttttcca tgacacttcc actcttccgt tttgatcata
     4681 ttgaattcct ttaatattta caattttgtg ctcttttcta tatttttcat catctatctc
     4741 aacaattcct tgatcatacc agtcatttag atctaactgt ttgacaatct tttcattatc
     4801 attatttgta atttcaataa caatacaatt tttcataacg taatctccct actctctata
     4861 ataaaaatca aaccaattcc acttctctaa aagaatatcc agtaaactta tttgactgaa
     4921 aaatattttt aaacttgtca gaaacaaaaa atctttgctt atactccttt aatcgaatta
     4981 tgtcatatcc ttcaagattt ttctttttca atacaaattt tcttacacct gctattttcc
     5041 ctctgacatt cgggttggga aaatcctcgc taaatcggtc aaaaacagac ttttcctcgt
     5101 caaaagcttc aataaaattg agcatattgg caatgcaaaa tccatctaag caaactccgt
     5161 taaatttaat aacttgtatc ggtaagtact gtattcctct gatttcattg atatcaagtg
     5221 ccgtgataag acgtggtgaa taaataggca gcatcaggta attttgaaga gcgtcatcag
     5281 gatccccatc atttcttccc ttttttacgc gaaaatatac attgtctttc caatcattga
     5341 taaatatacc ttgtttaaaa at
//
LOCUS       DAOOJC010000124         5338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12348_length_5338_cov_3.053379, whole genome shotgun sequence.
ACCESSION   DAOOJC010000124 DAOOJC010000000
VERSION     DAOOJC010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12348_length_5338_cov_3.053379"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLA01_04725"
     CDS             <1..416
                     /locus_tag="PLA01_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HPD00638.1"
                     /translation="LNFTKHLKFVKENGKYYIVCDYPEKPEGYDWHLGIKIFNKDGSY
                     ESYNHLTRVPGCQIPKEGSFKKEAIGISNISNVRSISISISLNHPQSGNTGLLDILFY
                     PDGSKKRCEFVPESGLYKNESYTDTFDFNKMFQWN"
     gene            complement(561..1682)
                     /locus_tag="PLA01_04730"
     CDS             complement(561..1682)
                     /locus_tag="PLA01_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HPD00639.1"
                     /translation="MRKRFSLIAVVVVLLNVLTATAFAATTETFYGGYSDSNYKGSIE
                     VDLTNISSKKNVILSETEAAEYFDDAETLDMIVDYGYEEYREITIKDIIEYYNDNGGV
                     PTYYADVAPVVVTAKLAMRTFYFSYKGDSVKYTFEPKYYSFGEYLYDFEDKQVYTQPP
                     EDWLYADGTTATLIEPGKYIFVVRDDGFVFPSSAGIFCVHIGDKLSEDPAPEELENPV
                     PEEPETQVPKDLNNVNVNPTTSKVLVNGKVVEFEAYNIDGYNYFKLRDIALAVNNTEK
                     NFEVTWDGVNNAINLISNKPYTPSGGELAKGDGTAKVAIPTTSKIYKDGKEIFLTAYN
                     INGNNYFKLRDITKAFDIGVTWDSATSTIGIDTSISYVE"
     gene            complement(1884..3020)
                     /locus_tag="PLA01_04735"
     CDS             complement(1884..3020)
                     /locus_tag="PLA01_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014522635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPD00640.1"
                     /translation="MNRYIVAFFLFSFLGWLWESIYCTIQECKWANRGFLYGPICPIY
                     GFGSLMGFFIYDSIKAGNLPNIEWWAILILGFVISMILEYPTSWALEKLFKARWWDYS
                     DLPLNINGRTSVPTSVAFGFGSILVMKVLIPWADSGINSMSEPLLNFLAIILVSIISI
                     DTTLTISSLTDFENYVASIDEEFQNHMTNIVDRVYGYRNSLYNKAIQRIAVFKLSGRK
                     NRIAKQIREKRFDDLIKDYYKSDEVRKMGESIQHGSTTTLEHCENVAWISYLINKKLH
                     LNADEKELIEAAMLHDISHHDWNNADVSRKIQNSDDSDVASDNTEGHFGIPEKEQEAI
                     RSYMWPLNITKIPKSKEALIVSLADKYCALIERIRLNKHFGLRH"
     gene            complement(3361..4476)
                     /gene="ablA"
                     /locus_tag="PLA01_04740"
     CDS             complement(3361..4476)
                     /gene="ablA"
                     /locus_tag="PLA01_04740"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HPD00641.1"
                     /translation="MEESIETSLEPEPYSTEWYDWKWQFRNRITTVEQLEKVIHLSEK
                     EKQDISFCLNNFRMAITPYYASLMDPVDPKCPIRMQAVPTINEINVLSYELADPLDED
                     RDSPVKNIVHRYPDRVLFLVTHMCSMYCRHCTRRRTVGEEDYAIGDKEIDAAVDYIAK
                     NKAIRDVLISGGDPLTLDDEKLEKIISKLRKIGHVEVIRIGTRVPVVLPMRITPELLA
                     MLKKYQPIWINTHFNHPKEITPASIKACTDIVDAGIPLGNQSVLLRGINDNTETMKEL
                     LLKLVKIRVRPYYLYQCDLSQGLGHFRTEVEKGIEIIKSLTGYISGYAVPKFVIDSPH
                     GGGKIPINPEYVVSADDGEIVMINYAGKVYTYPHIMY"
     gene            complement(4495..>5338)
                     /locus_tag="PLA01_04745"
     CDS             complement(4495..>5338)
                     /locus_tag="PLA01_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPD00642.1"
                     /translation="ELPVKLLENKPDIIFNIAEGICGRGREAQVPALLNFYKIPYTGS
                     DETTLCIALDKALTKRILSTYGIKTPKYRIITKDQNQLTGRFYFPAIVKPNAEGSSKG
                     ISDVAVVTNRRELEKLALKNISLYKQDMLVEEFISGREFTVGIFGNGSDIHVFSPMEI
                     CYVNQVNQYNIYSYNVKRNYKEFIRYECPANISKETEAEMTNAARKIYEALCCRDFAR
                     IDFLLSPQGKLYFIEINPLPGLAPGYSDFPMIAEFNGMDYNSLVWNVLKSALKRYGMK
                     SYID"
BASE COUNT         1586 a         1126 c          902 g         1724 t
ORIGIN      
        1 tgcttaactt tactaagcat cttaagtttg tgaaagaaaa tggaaagtac tatatagtat
       61 gtgactatcc ggaaaagcct gaaggctacg actggcattt aggaataaaa atatttaaca
      121 aggatggttc gtatgagtca tataatcatt taacccgtgt accaggctgt caaataccca
      181 aagaaggttc ctttaagaaa gaagctattg gtatcagtaa tataagcaat gttagatcta
      241 ttagtataag tatttcactt aatcacccac aatcaggcaa tacgggtttg ttggatatac
      301 tcttttaccc tgatggctca aaaaagagat gtgaatttgt accggaatcg ggattatata
      361 aaaatgagag ttacacagat acttttgact ttaacaaaat gtttcaatgg aactaatctt
      421 ctaaactcag attgaacttc aaattatcca aaatccttaa tcattcaaaa atcaaaaaag
      481 agttccccta tatcaattta ttaaagggaa ctctttacta ttataaagac cattctataa
      541 tggcttaatc aatttcactt ctattctaca tagcttatgc ttgtgtcaat tccgattgta
      601 ctggtcgcac tgtcccaagt tacgccaata tcaaaagctt tggttatatc tcttaacttg
      661 aaataattgt tgccgttgat gttataagca gtcaggaata tttctttgcc gtctttataa
      721 atcttcgaag tggtcggaat tgcaaccttt gctgtaccgt ctcccttagc cagctcaccg
      781 cctgacgggg tgtatggttt atttgagatt aggttgattg cattgtttac tccgtcccag
      841 gtaacttcaa aattcttctc tgtattgttt acggccagtg ctatatccct tagcttgaag
      901 tagttgtagc cgtctatgtt ataagcttca aactcgacaa ctttgccgtt taccaatacc
      961 ttagaagttg ttgggttaac gtttacatta tttaaatctt taggaacctg agtttcaggc
     1021 tcttcaggaa caggattttc aagttcttca ggagcaggat cttcagacag cttatcacct
     1081 atatgcacac aaaagatacc tgcagaacta ggaaaaacaa atccatcatc tctaacaaca
     1141 aaaatgtatt tacccggttc gattaaggta gcagttgtac catctgcata caaccaatct
     1201 tccggtggct gggtataaac ttgtttatct tcaaaatcat aaaggtattc accaaaggaa
     1261 taatatttgg gctcaaaggt atactttacg ctatctccct tatagctaaa ataaaaagtt
     1321 ctcattgcta attttgcagt aacaactacc ggtgcaacat ctgcataata tgtaggaaca
     1381 ccgccattgt cattgtaata ttctataata tctttgattg ttatctctct atactcttca
     1441 taaccataat caactatcat gtcaagggtt tcagcatcat caaaatactc tgctgcttcc
     1501 gtttcactca atataacatt tttctttgaa gagatgtttg tcaaatcaac ttcaatgctt
     1561 cctttgtaat tcgaatcgct ataccctcca taaaatgttt cagttgtagc tgcaaaggct
     1621 gttgctgtaa gtacattcaa aagtaccacg actactgcaa tcagtgaaaa acgttttctc
     1681 atttaatcct ctcctttatg gtttattggc aatattatgt agacaataat atttatcgac
     1741 atatatcgac ttattaataa ccataatatt atattatgta aatcaagaat gcttagagaa
     1801 ccctaaagaa agcacaaagg catgagtcac aaggtccgtc aggtccttca tgatcatgcc
     1861 ggtgatttgc ttggaataat attttaatgc cttaaaccga agtgtttatt taagcgaatc
     1921 ctttcaataa gagcacaata cttgtccgct aaagatacga tcaaagcctc tttgctttta
     1981 ggaattttcg taatattcag aggccacata taactgcgta ttgcctcctg ctctttttca
     2041 ggaataccga aatgtccttc tgtgttatca cttgcaacat cagaatcatc tgaattctgt
     2101 attttgcggg aaacatcggc attattccag tcatggtgag atatatcgtg aagcattgct
     2161 gcttcaataa gctccttttc atccgcgtta agatgcagtt ttttatttat aagataactg
     2221 atccatgcaa cattttcaca gtgttccagt gtagtggttg aaccatgctg aatagactca
     2281 cccatctttc ttacttcatc ggatttataa taatctttta tgagatcgtc aaaccgcttt
     2341 tcacgaattt gctttgctat tcggtttttg cgccccgaaa gcttaaaaac cgcaatacgc
     2401 tgaattgctt tattgtataa agagtttcga tagccgtaaa ccctgtcaac tatattagtc
     2461 atatgatttt gaaattcttc atctattgaa gcaacatagt tctcaaaatc cgtcagtgac
     2521 gatattgtta atgtagtatc tatcgatata attgaaacaa gtataattgc cagaaaattt
     2581 aataaaggct cagacataga attaattcca ctatccgccc agggtatcag aactttcatc
     2641 accagtattg aaccaaaacc aaaggcaaca gacgtaggca cgcttgtcct tccgttgatg
     2701 ttcagaggaa gatcgctata atcccaccat cttgccttaa acagtttttc aagagcccat
     2761 gatgtgggat attccaaaat catgcttata acaaagccaa gtataagtat cgcccaccat
     2821 tcaatattcg gcagattacc ggcttttatc gagtcatata tgaaaaagcc catcaatgac
     2881 ccaaatccgt agatcgggca aatcggtccg taaagaaaac cacggttagc ccacttacac
     2941 tcttgtatgg tgcaatatat actttcccaa agccagccga gaaaactgaa aaggaaaaag
     3001 gcgacaatat acctattcaa ttaatcactt ccttccgttt tttcatttta atacatccca
     3061 gaaaagttag atttgcttag atttataaat atactcaatc atcgacatat tatttaacag
     3121 cattatgtat attcaaaatt atatcagaaa tcacgatatt ttcaacgttc cgttttgcat
     3181 agttatgaat aaaaaagtgg gataaaagca agaaatccca gcataatcaa gagtatcctt
     3241 cttgctatgc cgggattaaa gcagtctttc agtgtctata tacttgtttt tgtagcatct
     3301 tccgttaaaa taaaaatttg aatattataa atttgccgct gttttaaatt ttacggcact
     3361 tcagtacata atatgcggat aggtgtatac ttttccggca taatttatca taacaatttc
     3421 accgtcatcg gcagaaacga catactccgg gtttatcgga atcttccctc cgccatgagg
     3481 tgaatcaatc acgaatttcg gaacggcata tccggaaata taaccggtca agctttttat
     3541 aatctcaatc cctttttcga cctcggtccg aaagtgtccc agcccttgac ttaaatcaca
     3601 ttgatataga taatacggcc tgacacgaat tttcaccaac tttaagagaa gctccttcat
     3661 ggtctccgta ttatcattga ttcccctaag aagaacgctt tggttaccca acggaatacc
     3721 tgcatccaca atatcggtgc aggcttttat ggaagcagga gtaatctctt tgggatggtt
     3781 aaaatgggta ttaatccaaa tgggctggta tttctttagc attgctaaaa gctcaggtgt
     3841 gatacgcata ggcagaacaa ccggcactct tgttcctatt cgaatgacct ccacatggcc
     3901 gatctttctt agcttagaga ttattttctc cagcttttca tcatccagtg tcagcggatc
     3961 tcctccggat ataagcacat cccttattgc cttgttcttt gctatataat ccactgctgc
     4021 atcgatctct ttatcaccga ttgcataatc ttcctctcct acggtccgtc tcctggtaca
     4081 atgccggcaa tacatggagc acatatgggt aactaaaaac agcaccctgt ccgggtagcg
     4141 gtgcacaatg tttttcaccg gactgtcccg gtcctcatcc agtggatctg ccagttcata
     4201 ggaaagtaca tttatttcgt ttatcgtcgg aactgcctgc atccggatag gacatttcgg
     4261 atccacagga tccatcaagg atgcatagta cggtgtgatc gccattcgaa aattatttaa
     4321 acaaaaggat atatcctgtt tttctttctc gcttaggtga attacctttt ccaactgttc
     4381 tacggttgtg attctgtttc taaactgcca tttccaatcg taccattcgg tactataagg
     4441 ctccggttcc agagatgttt caatgctttc ttccattttt attatccccc taaattaatc
     4501 tatataagat ttcatgccgt atcttttcag agcacttttc agcacattcc ataccaaaga
     4561 gttatagtcc attccgttga attccgctat catcggaaaa tcactataac ccggtgccaa
     4621 tccgggtaag ggattaatct caataaaata aagcttccct tgtggagaaa ggagaaaatc
     4681 gattctcgca aaatccctgc agcacaaagc ttcatagatt tttcttgcag catttgtcat
     4741 ctcagcttct gtttctttac taatattcgc agggcattca taccggataa attctttata
     4801 atttcttttt acgttatagc tataaatgtt atattgattg acctgattca cgtagcatat
     4861 ttccatcgga gaaaatacat gaatatcact gccgtttccg aatataccca cggtaaattc
     4921 acggccgctg atgaattcct caaccaacat gtcctgtttg tacaggctta tgttctttag
     4981 cgccagtttc tccagttctc tgcggttggt aaccaccgca acatcggata ttcctttgct
     5041 ggaaccttcc gcattgggct taacaatcgc agggaaataa aaccttccgg tcaattgatt
     5101 ttgatctttg gtaatgattc gatacttcgg tgttttaata ccgtacgtag aaaggattct
     5161 ctttgtcaat gccttgtcca gtgcaatgca caaggttgtt tcatcagatc ccgtataagg
     5221 gattttataa aaattaagca aagcaggcac ttgtgcttca cggcctcttc cgcaaattcc
     5281 ttccgctatg ttaaagataa tgtcgggctt attctccaaa agcttcaccg gcagttcc
//
LOCUS       DAOOJC010000125         5332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12360_length_5332_cov_3.795717, whole genome shotgun sequence.
ACCESSION   DAOOJC010000125 DAOOJC010000000
VERSION     DAOOJC010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12360_length_5332_cov_3.795717"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLA01_04750"
     CDS             <1..357
                     /locus_tag="PLA01_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00643.1"
                     /translation="LGCTWDGALERYLQWHPEIKKIVFALDNDYLAKDKDGCYRNWGQ
                     MAAMKWCDKYSEKGYEVAIHRPHLKDFNQDLIENRKGRTVAELDAMREDELKAEFEKD
                     AVDEPNEELDMEEVEP"
     gene            564..1064
                     /locus_tag="PLA01_04755"
     CDS             564..1064
                     /locus_tag="PLA01_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00644.1"
                     /translation="MKDKPKSNTPKTQKRSVVFSTDTLEQIEKLAIKKNVFASDIIRE
                     FVEKGLAVNGYEENIDMITAIIHQEIETSIKSLANRLAGMINRLTIISAAGYYANIAI
                     ISDLIDKDRYSSFEKIEKLARKRALVYANMKSADAIAVFMDDEEMKKAIAEVKGGTAA
                     YVDFDV"
     gene            1045..2280
                     /gene="mobP3"
                     /locus_tag="PLA01_04760"
     CDS             1045..2280
                     /gene="mobP3"
                     /locus_tag="PLA01_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP3 family relaxase"
                     /protein_id="HPD00645.1"
                     /translation="MSILMYKQRFRPPNYKKTPKCNYAHIRYIATRPGASKNEGMRHG
                     LFGKLYPGNLVEFETWQEIAREVRELSYKKVNIFRSVISFTPQTAAELSLKDYKAWED
                     YIEKHIAILAQKNGISLKNLSWVCAHHNEVSHPHIHVVFWDKNQKTMKNFVKPEVADS
                     IRIQLIKETFADKIADYCRAKENSKSALKETTDQLVKDFDDYMKSIYPKEYKYLKELV
                     GKIDEDDLAAIPLDGVLNGINLSPLSVRLFQLKDIMPKKGRLYYQLLPKEVKEAIDEL
                     IADLKQSVPYIKDLIDEYAEIKSKLAMLYDTDPENINKHKDKAVDEMDKLIANKVLGV
                     VKTILNKERELSNVEFTEARKIYYTEQMVYEILIMLEQNTSDLNMDYDDAEKAMSTEL
                     SKRAKKELYLNNKDKSMEK"
     gene            2373..3056
                     /locus_tag="PLA01_04765"
     CDS             2373..3056
                     /locus_tag="PLA01_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00646.1"
                     /translation="MVSKKQLYSELFKRLKKVQLTIKEPEISDVTAEIFNDTGGLVAV
                     ITADDRLIYSGCQCESANIIVEIASDLKKQFNLSSDLPVKPIGNLPNGYMKLYEVGDI
                     VMGCRLSPLIGYEYATRKKGMDYDKSEVYYYARQFLCFKEAQQDFAERCGFIEKQQKV
                     KRDNLFSNAELSLLLSCCIERVKLDAALTSEVEKAIGDVISKIEQILPAPPTDSEQDI
                     ESENVRCFR"
     gene            3053..3715
                     /locus_tag="PLA01_04770"
     CDS             3053..3715
                     /locus_tag="PLA01_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00647.1"
                     /translation="MRKFSNELYRAFLGRAYTLGYTVVEFETVGQPVEFYKGREYICS
                     LMPDGEIHYKDNTAVRDDVFRLSELFYSMKHAYDLYEKAENLPFDSVKNYKVLCEFGN
                     FLLAAMMDNNDQLRFVTWRYSYNRDSVAYGHYFDTDYDGARQDFAVRAGLIDEKKLFK
                     ENELVTLYEACIFRGRNDREISFDDEKRLMNVMNRIQENIPNLSLDCHEQNHEAESEL
                     DR"
     gene            3742..>5332
                     /locus_tag="PLA01_04775"
     CDS             3742..>5332
                     /locus_tag="PLA01_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPD00648.1"
                     /translation="MKNTLKRAMAIVTAIALILTLFGGAAYAAFKVFPDAANHWARET
                     IEKLSQEGIVNGYPDGTVRPDATITRSEFVSLLTYSLKLEGYTHTGPDTFDDTDKHWA
                     ESFIEALVKNKVIAKEDYGKDFKPDQPISRMEMIRMMVRSIGKADMAKIMTGDTPFVD
                     EGQITKEDKGYINVAEKYGLSYGYPDKTVRPMAYSTRAEAFAMILRRFAAMERISQEQ
                     ADNNKEQENTKPDEKSNRSNSGGGSASYPAAKVEISLPNYSHIDTPFEVKTTLSHVKN
                     LEWTIQKKDTESLATDVLEGTLNKDGGKICIKEAGDYKITATATNQGNRKYTFSKEIK
                     IYPVPDISIVLDDKTHIDKEVEVKAVVKDNKTVTWRLYKDGKQVKWSEYIEGNLNNDS
                     GSISFKESGKYSLVAAVIDETNREFTGKGDIEIYPVPQLTLNMPKTAHTDSTVNVTVE
                     SKNLGNLNIVWQLEKDGKQVSLKDCINGNLTNEGGNIQFKEKGQYTLRAVVIDITGRR
                     FEAAAPITVFPVASFAFSFFFG"
BASE COUNT         1844 a          898 c         1211 g         1379 t
ORIGIN      
        1 ctcggatgta cctgggatgg cgcccttgaa cgatatttgc agtggcatcc tgaaatcaaa
       61 aaaatagtat tcgccttgga caatgactac cttgctaaag ataaagacgg atgttataga
      121 aactggggtc agatggctgc aatgaaatgg tgtgataaat acagcgaaaa aggctacgaa
      181 gtagcaatcc acagaccgca tctcaaagac tttaaccagg atctgattga aaaccgtaaa
      241 ggaagaactg tagctgagct tgacgccatg cgtgaagatg aactgaaagc cgaatttgaa
      301 aaggatgccg ttgatgagcc gaatgaggaa cttgacatgg aggaggttga accatgaact
      361 ttcttttcat cagtatgaaa ccttcccttt tgggaagccc cgaacctaaa ggggaaaggg
      421 tttaatccct tcccttttga ggtaaggcag gaatagtggg gttcccaccc cacgagaaaa
      481 cagcttaccg gcagtgccgg taacggggtt cttgcaatat taacagtcac ccaatggggt
      541 gacaagcgga aaggaggagt attgtgaaag ataaacctaa aagcaacact ccaaaaactc
      601 agaaaaggtc agtggttttt tctaccgata ctttagaaca gattgaaaag ctggcaataa
      661 agaagaatgt atttgcttct gatattatca gagaatttgt ggagaaagga ttggcagtca
      721 atggatatga agaaaacata gatatgataa ccgccatcat tcatcaggaa attgaaactt
      781 caattaaatc actggctaac cgtcttgcag gaatgattaa cagacttaca attatttcag
      841 ccgcaggata ctatgcaaat atcgccatta tatcggactt gattgataaa gacagatact
      901 cttcttttga aaaaatcgaa aagctggcaa gaaaacgtgc tttagtttac gccaatatga
      961 aaagtgctga tgcaatcgca gtctttatgg atgatgaaga aatgaagaaa gcaattgcag
     1021 aagttaaggg aggaacagct gcttatgtcg attttgatgt ataagcagag atttcgtcct
     1081 ccaaattata aaaaaacacc caaatgcaat tatgcccata tccgatatat tgccactcgc
     1141 cctggagcat caaagaatga gggcatgcgt cacgggttgt ttggcaaact ttatcccggt
     1201 aacttggtag agtttgaaac ctggcaagag atagcaagag aagtgcggga gctgtcatat
     1261 aaaaaggtta atatatttag aagcgtcatt tcatttacgc cgcaaacagc agccgaattg
     1321 agtttgaaag attataaggc gtgggaggat tacattgaga agcatattgc gattcttgct
     1381 caaaaaaatg gcatcagtct taaaaatctg tcctgggttt gtgcacatca taatgaagtc
     1441 agtcatcctc atatccatgt agtcttctgg gacaaaaacc aaaagactat gaagaatttt
     1501 gttaagccgg aagtagccga cagcattcgt atacagctta taaaggaaac ctttgcagat
     1561 aaaattgcgg attattgcag agctaaggaa aacagtaaat cagcactgaa agaaactaca
     1621 gatcaactgg taaaagactt tgatgactat atgaaatcca tttaccctaa ggaatataaa
     1681 tacttaaaag aacttgtagg gaaaattgat gaagatgatt tagcagccat tccgcttgat
     1741 ggagtattga acggtatcaa tctatctcca ttatcggtcc gtctgtttca gcttaaagac
     1801 ataatgccga aaaagggaag gctttattat caactgcttc cgaaagaagt taaagaggca
     1861 attgatgaac tgatagctga tttaaaacaa agtgttccct acattaaaga tttaatagat
     1921 gaatatgcag agataaagtc aaagcttgcc atgctttatg atacagaccc tgaaaacatc
     1981 aacaagcaca aagataaggc agtagatgaa atggacaaac tgattgccaa taaagtgctt
     2041 ggtgttgtca aaacaatatt aaataaggag cgtgagcttt caaatgtaga atttacagaa
     2101 gccagaaaaa tttattatac cgaacagatg gtttatgaaa tcttgattat gctggaacaa
     2161 aacacatcag atttaaacat ggactatgat gatgcagaaa aagcaatgag tacagagctg
     2221 tcgaaaaggg cgaaaaaaga actatatctt aataacaagg acaagagtat ggagaaataa
     2281 gttttccata tcctttttga ttaacaggaa aggaaaatga taaaatgcct gactaaaaca
     2341 atatcaatat cctgaatgaa agggtgataa ttttggtttc aaaaaagcaa ttatacagcg
     2401 aactttttaa gcggttaaaa aaggtacagc taactattaa agaacctgaa atatccgatg
     2461 taacagcaga aatttttaac gataccggag gactggtagc cgttataacc gcagatgaca
     2521 ggctgatata tagtggatgc caatgtgaaa gtgccaatat tatagtggaa attgcttcgg
     2581 acttaaaaaa gcagtttaat ctttcctcag acttgcctgt taagccgata gggaatctgc
     2641 caaacgggta tatgaagctt tatgaagttg gagacattgt aatgggctgc cggttgtccc
     2701 ctctgattgg ttatgagtat gctacccgga aaaaaggaat ggactatgac aagtcggaag
     2761 tctattacta tgctcggcag tttttatgtt ttaaagaagc acaacaggat tttgcagaaa
     2821 gatgtggctt tatagaaaaa cagcaaaaag tcaaaaggga taacctgttt tccaatgccg
     2881 aactttcact tctgctatct tgctgtatcg aacgtgtaaa gttagatgct gcacttacat
     2941 cagaggtaga gaaagctata ggcgatgtta tctccaaaat tgagcagatt ttgcctgccc
     3001 caccgacaga tagtgaacag gatattgaaa gcgagaatgt gaggtgcttt cgatgagaaa
     3061 atttagtaac gaattgtatc gggcattttt aggacgagcg tacacacttg gatatactgt
     3121 ggttgaattt gaaactgtcg gccaacctgt ggaattttac aaaggcagag aatatatttg
     3181 ttctttgatg ccggatggtg aaattcatta taaagataac acagcagttc gtgatgatgt
     3241 attccgatta tcagaattat tttattccat gaaacatgct tacgatcttt atgaaaaagc
     3301 tgaaaatctg ccgtttgata gtgtgaaaaa ttacaaggtg ctttgtgagt ttggaaattt
     3361 tcttttggca gcaatgatgg ataataacga tcagttgagg ttcgtaacgt ggaggtattc
     3421 gtataaccgt gacagtgtag cttatggaca ctactttgac accgactatg acggagcaag
     3481 gcaggatttt gcagtacgtg ccggtttgat tgatgagaaa aaactgttca aagaaaatga
     3541 actggtaact ttatatgaag catgtatttt caggggcaga aatgacagag aaatcagctt
     3601 tgatgatgaa aagaggttga tgaatgtaat gaacaggatt caagaaaata ttccgaattt
     3661 gtcattggac tgccatgagc aaaatcatga agcagagagc gaattggata gataaattta
     3721 aataaacgga ggaaattgaa tatgaaaaat acattaaaac gggcgatggc aattgttact
     3781 gctatcgctc ttattttaac cctttttggc ggtgctgctt atgctgcttt taaagtattc
     3841 cccgatgcag cgaatcattg ggcaagagaa acaattgaaa agctatcaca ggaaggcatt
     3901 gtaaacggat atcctgacgg tacggttcgc ccggatgcga ctattacccg cagtgaattt
     3961 gtaagtttgc tgacatatag cttaaagttg gagggttata cccacaccgg ccccgatacc
     4021 tttgatgata cggacaaaca ttgggcagag agtttcattg aagctcttgt caaaaacaaa
     4081 gtaatcgcaa aagaagatta cggaaaagat tttaagcctg accagcctat cagccgtatg
     4141 gaaatgatcc gcatgatggt acgttccata ggcaaagcag atatggctaa gatcatgaca
     4201 ggagatacgc cttttgttga tgagggtcaa attactaaag aagataaggg atatatcaat
     4261 gtagcggaaa agtatggttt gtcctatggt tatcccgata agaccgtgcg tccgatggcg
     4321 tacagtacca gagcggaagc ctttgcgatg attctgcgtc gctttgcggc tatggagcgt
     4381 atctcacaag aacaggcaga caacaataaa gagcaggaaa acaccaagcc ggatgagaag
     4441 tcaaatcgca gtaatagcgg tggtggcagc gcaagctatc cggcggcaaa ggtagaaatc
     4501 tcactgccaa attatagcca tatcgataca ccgtttgagg taaagacaac tttaagccat
     4561 gtgaaaaatc ttgagtggac aattcaaaag aaagatacag agtcacttgc aacagatgtt
     4621 ttggaaggaa cactcaacaa ggatggcggt aagatttgca ttaaagaggc gggagattat
     4681 aaaattacgg ccacagcaac caatcagggc aacagaaaat acactttcag caaggaaatc
     4741 aaaatttatc ctgtaccaga tataagtata gttcttgacg acaagacgca tatcgataaa
     4801 gaggttgaag tcaaagcggt agtgaaagat aacaaaactg taacatggcg tctttacaag
     4861 gacggaaagc aagtaaaatg gagtgaatat attgagggca atctgaataa tgatagtggt
     4921 tctatcagct tcaaagaaag cggcaagtat tcccttgtgg ccgccgtcat tgatgaaacg
     4981 aaccgagaat ttacaggaaa aggcgatatt gagatctatc ctgtaccaca gttgacacta
     5041 aatatgccaa aaacagcaca taccgactca acggtaaacg taacagtaga aagcaagaat
     5101 ttaggaaatc ttaatattgt ctggcagctt gaaaaggatg ggaagcaggt tagtttaaag
     5161 gactgtatca atggaaatct taccaatgaa ggcggcaata ttcaattcaa agaaaaaggc
     5221 cagtatactc ttagagccgt tgttattgac ataacgggca gaagatttga agctgctgcg
     5281 cctattactg tttttcctgt ggcgagcttt gccttttcct tctttttcgg ta
//
LOCUS       DAOOJC010000126         5313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12416_length_5313_cov_3.180677, whole genome shotgun sequence.
ACCESSION   DAOOJC010000126 DAOOJC010000000
VERSION     DAOOJC010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12416_length_5313_cov_3.180677"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..299)
                     /locus_tag="PLA01_04780"
     CDS             complement(165..299)
                     /locus_tag="PLA01_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00649.1"
                     /translation="MSSLAQTIIYLVAAIAFLALSYFLMVRSANKKLESIKTKNKKRK
                     "
     gene            507..1577
                     /locus_tag="PLA01_04785"
     CDS             507..1577
                     /locus_tag="PLA01_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00650.1"
                     /translation="MRTTVILGAGFSKNSGLPVQKEIPALLIKENAEDNFENAVSGVL
                     RRFMEDVFCYGKEIYPQLDDLLTCLDISISSGHHLGIKYSTEHLWAIKRFLVYRVFSI
                     LEGSFRYSCGIDRLVRFFVQERPQDTGFLVLNWDTVLERYFAGINNDIAVDYCNNGRN
                     LTGDEDGTGKKKIKTIKVHGSSNWLYCDNCRTLLNDMDCSVKPIRKAGFRKEDFDLFD
                     EMKGIVNENELSIGTVCNICEDNVSAHIATQCYRRSFRKSTFPLVWSEAEKMLADSEK
                     WVFIGYSLPQADYEFKHLLKISEHKLAHKKSKGLSIDVVLLDSDNAIENYKCFFGDRV
                     KRIFNGGIEEYINYLSFFHKKP"
     gene            1628..2239
                     /gene="yihA"
                     /locus_tag="PLA01_04790"
     CDS             1628..2239
                     /gene="yihA"
                     /locus_tag="PLA01_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPD00651.1"
                     /translation="MELNININNAKHEITAVKTDQYPKSDKPEIAFVGRSNVGKSSLI
                     NSMLNRKNLARVAATPGKTRVINFYNVDNILYFVDLPGYGFAKVSKAEKESWGFVVET
                     YLKSRKQLKLVVLLVDIRHSPTDDDILMHEWLKSNGIPYLIVSTKLDKVPRSKTAERV
                     CDIRKVLGNCDNVKVIPFSSETKQGRDELWKEIKLQLGCEVNQ"
     gene            2366..3448
                     /gene="ytvI"
                     /locus_tag="PLA01_04795"
     CDS             2366..3448
                     /gene="ytvI"
                     /locus_tag="PLA01_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HPD00652.1"
                     /translation="MNQKVKFILKIILTLVGTFIGIILTLKLSVYVAPFIIAFVISSI
                     IEPLIRFLMKKAKFPRRLAAIVSLLLVLSVISVLMLLFFSRLYNEVVSLTNERPQYLI
                     EIYQNVSAFIRRSFDAYLNLPDDITSYITNMISNLSQSVSKVFDPFIKGLYNAAISIP
                     QVIIFILVTVLATYFFSSDRDRIYESIKHNVPEFILEKAISIKDNIFMAFFGYIRAQL
                     ILMSITFTELSIGFAIIGIRRFLLLALVISFIDAFPVLGTGGVLVPWAIYEFITRDFR
                     MGISLIILYVIVLVIRQMIEPKILGQQIGLHPLITLMAMYLGTQFYGIVGFILGPIIV
                     LLFKNTVSGFIKQKSIKEIINEFNNK"
     gene            complement(3630..4334)
                     /gene="sigK"
                     /locus_tag="PLA01_04800"
     CDS             complement(3630..4334)
                     /gene="sigK"
                     /locus_tag="PLA01_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HPD00653.1"
                     /translation="MLGLLFSSLIDIINNIFFLLGYISNVNSFPQPLTHEEEQYYLQR
                     LREGDEEARNILIERNLRLVAHIVKKYNSTSNECDDLISIGTIGLIKAIATFNHEKGT
                     RLVTYAARCIENQILMQIRSNKKMQSEVSLQDPIGIDRDGNEITLIDVIGNESESIVE
                     EVELKMQIKRLYNKMKDVLKKREKLVLELRYGLINGTSKTQREIAKMLGISRSYVSRI
                     EKKAIKKLGKELKPDA"
     gene            complement(4405..>5313)
                     /locus_tag="PLA01_04805"
     CDS             complement(4405..>5313)
                     /locus_tag="PLA01_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPD00654.1"
                     /translation="SKPDFDPNEVARYLESPDNELFNRAVASYNLGSIFKIIDLASAY
                     SKKIDPSMYYFCPGYVTLGDKEFRCSAYARGGHGLIDLHEAFASSCNPYFIELGIRIG
                     PDAIIDTAKKFGLGSVTGIDAQGISESPGNLPDPSKYLTYGDTANIAIGQGDVMATPL
                     QVADMVATIANGGIKNKVNIVDSIIDSEGNPVKIIRKNEGQRIIEKNVCDSIKQLMEE
                     VTVSGTGTRANLGEYGGAGGKTGSAETGQYINGSKVVHAWFAGYFPRLDPKYSVSVFI
                     ENGKSGGSIAAPIFEEIAKNILIMGY"
BASE COUNT         1615 a          894 c         1074 g         1730 t
ORIGIN      
        1 ttaagaatat acaggattta cttttggaat tatattactt catgcgatag ccctggtgat
       61 ggtatgaaaa gacctattgt atgctggttt gagggtacga aaacaaagcc taagaaacgt
      121 tatccttaag ctttattaat attattaagc ttgaataaat acaattattt tcttttttta
      181 ttctttgttt ttatactttc aagcttttta tttgcagaac gcaccattaa aaaatatgaa
      241 agagccagga aagcaattgc tgctactaaa taaataattg tttgtgccaa actagacata
      301 ttgtatctat cacctccact gataattgaa attttaccac acatcaaaat ttttatcaat
      361 agccctgttg cggcttgggt gtacgatttt taattatcac tgataaaatc cggatattta
      421 agaaaaacta tattagttgg aagaaagcaa atttaattta caatatgctt tcgatgtttt
      481 ataaaaagtt gacggtggag atttgagtga gaacaacagt aatccttggt gctggttttt
      541 ccaagaattc cggactccct gtgcagaaag aaatacctgc tttattgata aaagaaaatg
      601 ctgaagacaa ttttgaaaat gctgtatccg gtgtgttaag aaggtttatg gaggatgtat
      661 tttgttatgg gaaagagata tacccccaac tggatgattt gcttacatgc cttgatatat
      721 ccataagttc ggggcatcat ctgggaataa agtattctac tgaacaccta tgggctatta
      781 aaaggttttt ggtatatagg gtattttcca ttttggaagg aagcttcagg tattcctgcg
      841 gtatagacag gttagttaga ttcttcgttc aagagaggcc tcaggacaca ggttttttgg
      901 tactaaactg ggataccgtt ctggaaagat attttgccgg gattaataac gacattgctg
      961 ttgattattg caacaatggc agaaatttaa cgggtgatga agatggcaca ggaaaaaaga
     1021 aaataaaaac aattaaggtt catgggtcgt ctaactggct ttattgtgac aattgcagga
     1081 cattgttaaa tgatatggat tgcagtgtaa aaccgattag aaaggccgga ttcaggaaag
     1141 aagattttga tttgtttgat gaaatgaagg gcatcgtgaa tgaaaatgag ctttcaatag
     1201 gtacagtatg taatatatgc gaagataatg tatcggcaca tatagcaacc cagtgttata
     1261 gaagatcctt tagaaagagt acttttccgc tggtatggag cgaagccgaa aaaatgcttg
     1321 cggattccga aaagtgggtt ttcataggat attctttgcc ccaggcagac tatgaattca
     1381 agcatctgct taaaatatcc gagcacaagc ttgcacataa aaagagcaaa ggactttcta
     1441 ttgatgttgt tttgcttgac agtgacaatg caattgaaaa ttataagtgc ttttttggag
     1501 acagagtaaa aagaattttc aatggcggaa tagaagaata tatcaattac ttgtcttttt
     1561 ttcataaaaa gccttaatta tttatgaaaa taatgctata ataccattta ataaaactgt
     1621 aaggagaatg gaattgaata taaatattaa taatgctaaa catgagatta cagctgtaaa
     1681 aactgaccaa tacccaaaaa gtgataagcc ggagattgct tttgtgggga ggtctaatgt
     1741 gggtaaatcc tcactcatca attcaatgct aaacagaaaa aatttggcaa gagttgcagc
     1801 aactccaggg aaaacaaggg ttataaactt ttataatgtt gataatatat tatattttgt
     1861 ggatctgccc ggttatggct ttgcaaaggt atcaaaggcg gaaaaggaat cctggggctt
     1921 tgttgtggaa acctatttga agtcaagaaa acaacttaag ctggtggttt tgcttgtgga
     1981 catcaggcat tctccaactg atgatgacat acttatgcac gaatggctta aaagcaatgg
     2041 cataccttat ttgattgtat ccactaagct tgacaaggtt ccccgttcga aaacagcgga
     2101 aagggtctgt gacataagaa aggtactggg aaattgtgat aacgtaaaag tgataccttt
     2161 ttcatcggaa acgaagcagg ggagagacga gctttggaag gaaatcaagc tgcagctggg
     2221 gtgtgaagtc aatcaatagc cggacttatt ttgaaatatg ttgaaatttg catgtggttt
     2281 aaaggagaag tattgagtga tatagaaatt atattaatat gattatttaa tgtataatag
     2341 taactgagaa taagggtggg gttgaatgaa tcaaaaagtg aagtttattt tgaagattat
     2401 actgacgctt gtaggaacgt ttatcggtat aatattgacg ttgaagcttt cggtttatgt
     2461 cgctcccttt ataattgctt ttgttatttc ttccataatc gaaccattaa tcaggttttt
     2521 aatgaaaaag gccaaattcc ccaggaggct tgcggcaata gtttctttgc ttttggtttt
     2581 atcggttata tcagtgctga tgctgttatt tttttcaagg ctttataacg aagttgtgag
     2641 ccttacaaat gaacggccgc agtatttaat tgaaatatac cagaatgtat cggcattcat
     2701 aagaagaagt tttgatgctt atttaaatct tcccgacgat attacatcct atatcaccaa
     2761 catgatttca aacctttccc aatccgtatc gaaagtattt gatcccttta taaaaggtct
     2821 ttataatgca gcaatttcga ttcctcaggt aattatattt atacttgtta cggttctggc
     2881 aacctacttt ttttccagtg acagagacag aatatatgag tctattaagc ataatgttcc
     2941 ggagtttata ctcgaaaaag caataagcat aaaagataac atatttatgg cgttttttgg
     3001 ctatataagg gctcagctca ttttaatgtc tattacgttt acggaactgt ctatagggtt
     3061 tgctattata ggaataaggc gatttcttct gttggcattg gttataagct ttattgatgc
     3121 atttccagta ctgggcacag gcggtgtgtt ggttccatgg gcgatatatg agtttataac
     3181 cagagatttc agaatgggca tatcccttat tatattgtat gtgatagtac tggtaataag
     3241 gcagatgata gaaccgaaga tactcggaca acaaataggg ctgcatcctt taattacact
     3301 gatggcaatg tatttgggga cacagtttta tggaatagtg ggttttattt taggtcccat
     3361 tatcgtacta ttgtttaaga atacagtatc cggatttatt aagcaaaaaa gtatcaaaga
     3421 gataataaat gagttcaata ataaatagtg tgtatattat tttatttgag aagggaaaaa
     3481 tgtaatagac attgtgaagt aagccagaga gcctgttaat acaacaaagg aagggagagt
     3541 atacaacctt ctcgaagcaa gttctccctg gcacgcaagg agtatctaca ggctcaaact
     3601 tttaaagctt aatttagaaa tatatttctt tatgcatcgg gtttaagctc tttccccaat
     3661 tttttgattg cctttttttc aattcgtgaa acataggatc tggatatgcc cagcattttt
     3721 gctatttctc tttgggtttt gcttgtgccg ttaataagtc cgtaccgaag ctccagaacc
     3781 aacttttctc ttttttttaa tacatccttc attttgttat acaggcgttt aatctgcatc
     3841 tttagctcaa cttcttctac tattgattcc gactcatttc caataacatc aataagtgtt
     3901 atttcgtttc cgtcacggtc tatgcctatg ggatcctgaa gtgaaacttc gctctgcatt
     3961 tttttattgg atcggatctg catcaaaatt tggttttcaa tgcatcttgc tgcgtaggta
     4021 accagtcttg tgcctttttc gtggttgaag gtagcaatag cctttattag ccctatagtg
     4081 ccaatggaga taaggtcatc gcactcattt gaggtagagt tatatttctt gacaatatgt
     4141 gctaccagcc tcaggtttct ttcaataaga atatttcttg cttcttcatc tccttcccta
     4201 agacgctgta aatagtattg ctcctcttca tgggttaatg gctggggaaa ggaattaaca
     4261 ttagaaatat atcccaaaag aaagaatata ttattaatta tatctataag agaagaaaaa
     4321 agtaagccaa gcaagataag gcacctccat aaataggcta ccttataatt atatgctgca
     4381 cttggaatac tggtgcaagt actattaata acccattatc aaaatatttt ttgcaatttc
     4441 ttcgaaaatc ggggctgcta tactcccgcc gctttttccg ttttcaataa aaacagaaac
     4501 tgagtatttt ggatccaacc tgggaaaata tcccgcaaac catgcatgta caactttgct
     4561 tccgtttatg tactggcctg tttccgcact tccggttttt ccgccggcac cgccatactc
     4621 gccgagattg gctctggttc ctgtgccgct taccgttact tcctccataa gctgcttaat
     4681 gctgtcgcat acgttttttt cgataattct ttgaccttcg tttttcctta taattttaac
     4741 cgggtttccc tccgaatcga taatggaatc cacaatatta accttatttt ttatacctcc
     4801 gtttgcgatg gttgctacca tatcggcaac ctgtagggga gttgccataa catcaccctg
     4861 gcctatggct atgtttgcag tatccccgta agttaaatat ttcgaaggat ccgggaggtt
     4921 gcccggggac tcgctgattc cttgggcatc aatccctgtt acacttccaa gaccgaattt
     4981 tttcgctgta tctataatgg catcaggccc tatcctgatt cccaattcaa taaaatatgg
     5041 gttacaggag gaggcaaagg cttcatggag gtctattaaa ccgtgcccgc ccctggcata
     5101 ggccgaacac ctgaattctt tgtctcctaa ggttacatac ccggggcaga aatagtacat
     5161 agaggggtct atttttttac tgtaggcact tgcaagatca atgatcttga atatggaccc
     5221 gaggttgtag gaggcgacag cacggttaaa aagttcattg tcaggacttt caagatacct
     5281 ggcaacctca ttgggatcaa aatccggctt gct
//
LOCUS       DAOOJC010000127         5303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12445_length_5303_cov_3.487805, whole genome shotgun sequence.
ACCESSION   DAOOJC010000127 DAOOJC010000000
VERSION     DAOOJC010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12445_length_5303_cov_3.487805"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..363
                     /locus_tag="PLA01_04810"
     CDS             82..363
                     /locus_tag="PLA01_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00655.1"
                     /translation="MSRLERYRKIRIMRRKCTFVCMLSFFIMFSGILAVDKSINEIMN
                     REKGVNIVSIKPYKNDYYYIKVFNKYFLVNTKPVKDDIDRFKEWLSDKF"
     gene            509..2320
                     /gene="lepA"
                     /locus_tag="PLA01_04815"
     CDS             509..2320
                     /gene="lepA"
                     /locus_tag="PLA01_04815"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPD00656.1"
                     /translation="MASERQKKIRNFCIIAHIDHGKSTLADRLLEATGVLTEREMEDQ
                     ILDTMDIERERGITIKAQAVRMVYKADDGEEYVLNLIDTPGHVDFNYEVSRSLAACEG
                     AILVVDAAQGIEAQTLANVYLALEHNLEILPVINKIDLPSAQPDVVKKEIEDVIGLDC
                     SNAPLISAKNGINIDEVLKSVVNAVPCPKGDVDAPLRALIFDSYYDSYKGVIVYIRVV
                     EGKVRIGDEIRMMYTKKDFLVTEVGYMKPGGHVACSELLAGDVGYIAASIKNVKDTRV
                     GDTVTSVENPAKEPLPGYKKVNPMVFCGIYPADGSKYGDLRDALEKLQLNDASLTFEP
                     ESSTALGFGFRCGFLGLLHMEIIQERLEREYDFDMVTTAPSVIYKIIKTSGEMFYIDN
                     PTNLPPTAEIDSMEEPIVKATIMSPSEYVGNIMELSQERRGVYKDMSYIDEGRVMLTY
                     EMPLNEIIYDFFDALKSRTKGYASLDYELLGYKESELVKLDIMLNGEIVDALSFIVHK
                     DKAYARGRRIAEKLKDTIPRQQFEIPIQACIGGKIIARETVKAYRKDVLSKCYGGDIT
                     RKKKLLEKQKEGKKRMRQVGSVEVPQEAFMSVLKLDS"
     gene            2425..3525
                     /gene="hemW"
                     /locus_tag="PLA01_04820"
     CDS             2425..3525
                     /gene="hemW"
                     /locus_tag="PLA01_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPD00657.1"
                     /translation="MCRDNSIPAYFNAMKKEMALYADKLRGYSIDTVFIGGGTPSYVD
                     EHNIYEIIAYMKNMFDMEKCREITIETNPGTLSFDKLISYRAMEINRLSIGLQAWQNR
                     LLSMLGRIHTVEEFIDNYNLARKAGFDNINVDLIFGIPGQTLCDWVETIDCTASLMPS
                     HLSCYSLIIEEGTVFGTRFEKGELVPVEDELDRKMYWHTIEKLSYLGYKHYELSNFAR
                     EGYECAHNMIYWKEREYIGFGAGSHSYLNGRRFNNVYGIDEYIGILNRECLPLENPID
                     IAKEDEMSEFMMLGLRLIEGVSVKEFKERFDKDVFEVFGSQIESLLHRGLIEAGNGFI
                     KLTRLGLTLLIRHLWSSYDILGGKNKLLGLSK"
     gene            3625..4680
                     /gene="hrcA"
                     /locus_tag="PLA01_04825"
     CDS             3625..4680
                     /gene="hrcA"
                     /locus_tag="PLA01_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HPD00658.1"
                     /translation="MLLDDRKKRILQSIIDDYISTAEPVGSRTVARKHEFGLSSATIR
                     NEMADLEEMGFIKQPHTSAGRIPSDQGYRFYVDQLMKASQLTVEEILSIKSTMDVKIN
                     ELNQLLKQVSAVMSKITKYTSMAATPERKNSILKTIQVVPVERGRALVIVVTNSGAVK
                     NSLISISETVSPEFLMYVSNIFNEKLSGLTIDQINMPVIREIEILIGPSQDILMPILN
                     GVVDCINQIDSSEVYLEGTVNMLNYPEFSDVDKLKEFLGLMDKKELLGRLLNELSQSN
                     GNVTISIGEENKIEKMKDCSLVTTTYKVGNIVIGTIGVIGPTRMDYSKVLAAVNFMKN
                     RIVDEVEKLLGKSSDDP"
     gene            4729..>5303
                     /gene="grpE"
                     /locus_tag="PLA01_04830"
     CDS             4729..>5303
                     /gene="grpE"
                     /locus_tag="PLA01_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPD00659.1"
                     /translation="MKEKGKDKQIEKEQDKCQINSNEDVRKSQNDDISAGEELDNNRE
                     MEQLRNELEEKTKKCEEYLNMLQRTAAEFDNYKKRTAKEMGSIYNDAAVDVVNAFLPV
                     VDNIERAVQASSKEVDAASLKEGIDLVYRQFKEVMGKLGVEEIKSLGEKFDPNLHNAV
                     MHVEDPEYGESVIIEEFQKGYILKDKVIRYS"
BASE COUNT         1764 a          767 c         1299 g         1473 t
ORIGIN      
        1 aataataaat aagatttaat aaataagaat tatgtgggaa taatagataa caggattact
       61 taagataaat ggggtttgtt gatgagtaga ttggaaaggt atagaaaaat aagaattatg
      121 aggagaaagt gcacttttgt ttgtatgctt tcttttttta ttatgttttc gggaatactt
      181 gccgtggaca agtctatcaa tgagattatg aacagggaaa agggggtgaa tattgtttca
      241 ataaagccat acaaaaacga ttattattat ataaaagttt ttaacaagta ttttttggtg
      301 aacacaaaac ctgtaaaaga cgatatcgat aggtttaaag agtggctttc ggataaattc
      361 tagatgttca tgggtgtata ctacgttttt tttattgtgc aaaaaattct tctttttgtt
      421 tgattaatat gttataataa tttgctgaca aacatataaa ctgttagatt cagaaatata
      481 ctccctaagc ttgtaggagg atgaatagat ggcaagtgaa agacaaaaga agataaggaa
      541 tttttgtata attgcacata tcgatcatgg aaaatcaacc cttgcggata gacttttaga
      601 ggccacaggt gttctcacag aaagggaaat ggaggaccag attttagata ccatggatat
      661 tgaacgtgag cgggggatta ctatcaaggc tcaggccgta cgtatggtat ataaagccga
      721 tgacggagaa gaatatgttc taaaccttat agataccccg ggacatgtgg attttaatta
      781 tgaggtatcc agaagtcttg cagcgtgtga aggagcaata cttgtagttg atgcagcaca
      841 aggtatagaa gcacagacac tggctaatgt ctatcttgct ttggaacaca accttgaaat
      901 actgcccgtg attaacaaga ttgatcttcc cagtgcccag cctgatgtgg ttaaaaagga
      961 aatagaggat gtcatagggt tggattgttc aaatgctcct cttatttctg caaaaaacgg
     1021 tattaatatt gatgaggttc ttaagagtgt ggtaaacgcg gttccatgtc cgaagggtga
     1081 tgtggacgcc cctcttcgtg cccttatttt cgattcttat tatgacagct ataagggtgt
     1141 tattgtgtat ataagggtag tcgagggaaa ggtcaggata ggcgatgaaa taaggatgat
     1201 gtataccaaa aaggattttt tggttacaga ggtgggctat atgaagcccg gaggacatgt
     1261 tgcctgttct gagcttttgg ccggagatgt aggctatata gccgcaagca taaagaatgt
     1321 caaggacacc agggtgggcg atacggttac ttcggtggaa aatccagcaa aggagcctct
     1381 tcccggttat aagaaggtaa atcccatggt gttttgcggt atttatccag cagatgggtc
     1441 aaaatacggt gaccttcgcg atgcattgga aaagctgcaa ctcaatgatg cttcactgac
     1501 ctttgagccg gaatcttcca ctgctctggg ttttggattc agatgcggtt ttttgggact
     1561 tcttcatatg gaaataatac aggaacgttt agagagggaa tatgattttg acatggtaac
     1621 tacagcaccc agtgttatat ataaaataat aaagaccagc ggcgaaatgt tttatataga
     1681 caatcccacc aatttgccgc ccacagcgga gatagacagt atggaggagc ctattgtaaa
     1741 ggcaaccatt atgagcccgt cggagtatgt gggaaatatc atggagttgt ctcaggaaag
     1801 acgcggagta tacaaggaca tgtcatatat tgatgaaggc agggtaatgc tgacttatga
     1861 gatgcccctt aatgaaataa tatatgactt ctttgatgct ttaaaatcaa ggaccaaagg
     1921 gtacgcatct ttggattatg agctgcttgg atataaagaa tcggagcttg taaagctgga
     1981 tataatgtta aatggagaaa ttgtcgatgc tttgtcattt attgttcata aggacaaggc
     2041 atatgcaagg ggcaggagaa ttgcggagaa gcttaaggat acaattccaa ggcagcagtt
     2101 tgagataccg atacaagcgt gtattggcgg taagattatt gccagggaaa cagtcaaggc
     2161 atacagaaag gatgttctgt caaagtgcta tggaggagac ataacacgta agaaaaagct
     2221 tttggaaaaa caaaaagagg gtaaaaaacg tatgcgtcag gtgggaagtg tagaggttcc
     2281 ccaggaagca ttcatgagtg tattgaagct tgactcttaa agaagatggt gttggcaatg
     2341 tcgaatactg ataaaaaact gggaatatac attcacatac ccttttgtaa agcaaagtgc
     2401 ctttattgtg actttaattc ttttatgtgc agggataatt ctatccctgc atattttaat
     2461 gccatgaaaa aggaaatggc tttgtatgcc gacaagctca gaggatacag cattgatact
     2521 gtgtttatcg ggggaggaac accctcctat gtggatgaac ataatatata tgaaataatt
     2581 gcatatatga agaatatgtt cgatatggaa aagtgccgtg aaattactat tgagaccaac
     2641 ccgggcactc ttagctttga caagcttata tcatacaggg caatggaaat aaacaggctg
     2701 agcattggcc ttcaagcgtg gcagaaccgt cttttatcta tgctgggaag aattcacacg
     2761 gttgaagaat tcatcgataa ttataatctc gcacgaaagg ccgggtttga taacattaac
     2821 gtggatctga tttttgggat tcccggtcag accttatgcg attgggtgga aacaatagat
     2881 tgtaccgcaa gcttaatgcc gtctcattta tcatgttata gcctgataat agaggaaggc
     2941 acggtattcg gaacaagatt tgaaaaagga gagctggttc ctgtggaaga tgagcttgac
     3001 agaaagatgt attggcatac gatagaaaaa ctaagttatt tgggatataa gcattatgaa
     3061 ttatccaatt ttgcaagaga aggttatgaa tgtgcacaca atatgattta ttggaaagaa
     3121 cgggagtata ttggattcgg tgccgggtcc cactcatatt taaatggccg gaggtttaac
     3181 aatgtttatg gcattgatga gtacatagga atcttaaacc gggaatgttt gcccttggag
     3241 aatcctatag acattgcaaa agaggatgag atgtcggagt tcatgatgct gggattaaga
     3301 cttattgagg gagtaagtgt caaagagttc aaagaaaggt ttgacaaaga tgtgtttgag
     3361 gtgtttggga gccagataga aagcctattg catagggggt tgattgaagc cggtaatgga
     3421 ttcattaagc ttacacgatt gggactgacc ttgctaatca ggcatttatg gagttcgtat
     3481 gatatacttg gcgggaagaa taagcttttg ggtttaagca aataataatc cacaaaaata
     3541 tcttgacaaa agaaacctag ggtggtattt taagaataga attagcactc agcaaggtag
     3601 agtgctaaca aaggggtgat aatgatgtta ttggacgata gaaagaagag aatacttcag
     3661 tcaattatcg acgactacat cagtactgcg gaacccgttg gctcaaggac agttgcaaga
     3721 aaacatgagt ttggactcag ttcggcaacc attagaaacg aaatggctga tttggaggaa
     3781 atgggattta tcaaacagcc ccatacttct gcgggacgta taccatcgga ccagggatat
     3841 aggttttatg tagaccagct aatgaaagcc agtcaattga ctgttgaaga gatactaagt
     3901 atcaaatcca ctatggatgt gaaaataaat gagcttaatc aactgttaaa acaggtttcg
     3961 gctgtgatgt cgaagattac aaagtatact tctatggcag ctacacctga aagaaagaac
     4021 agcattttaa aaaccataca ggtggtgccg gttgaaaggg ggagggcact tgttattgta
     4081 gtaactaatt caggtgcagt taagaacagc cttattagta tttccgaaac ggtttcgccg
     4141 gagtttttga tgtacgtttc caatatattt aatgagaagc tgtcaggtct tacaatagat
     4201 cagataaaca tgcctgtaat tagggagata gagattttaa tagggccttc tcaggacatt
     4261 ttaatgccta ttttaaacgg cgttgtcgat tgtataaatc agatagacag ctcagaggtt
     4321 tatcttgaag gtactgtaaa catgttgaac tatcctgagt ttagcgatgt agacaagtta
     4381 aaagagtttc tgggccttat ggacaaaaag gagcttttag gcagattgct taatgagctg
     4441 tcccaaagta acggcaatgt taccattagt attggcgagg aaaacaagat tgaaaaaatg
     4501 aaggattgca gtctcgtcac gaccacttat aaagttggga atattgttat tggaacaata
     4561 ggagttatag gtccgacgag gatggattat tccaaggttt tagcagctgt aaatttcatg
     4621 aaaaacagga tagtggatga agttgagaaa ttgttgggaa aaagttcgga tgatccctaa
     4681 ataattgtaa agctaaaaaa atacatcgat gagggaggtt atataatttt gaaagagaag
     4741 ggaaaagaca aacaaattga aaaagagcag gataagtgtc aaatcaacag taacgaagat
     4801 gtaagaaaga gtcaaaacga tgatatttct gccggggagg aacttgataa taacagggaa
     4861 atggaacagc ttagaaatga gcttgaggaa aaaacaaaga agtgtgaaga atatttaaac
     4921 atgctccaga gaacggcagc agagtttgat aactataaga aaagaacagc aaaggaaatg
     4981 ggatccatat ataatgatgc tgctgttgat gtagttaatg ctttccttcc ggtggttgac
     5041 aacattgaaa gggctgtaca ggcgtcctca aaggaagttg atgctgcatc cctgaaagaa
     5101 gggattgatc tggtttaccg ccaattcaaa gaagtcatgg ggaaacttgg agtagaggag
     5161 attaagtctt tgggtgaaaa atttgaccca aacttacata atgccgtgat gcatgttgaa
     5221 gacccggaat atggtgaaag tgttattatt gaggaatttc aaaagggtta tatattaaag
     5281 gacaaggtaa taagatacag tat
//
LOCUS       DAOOJC010000128         5267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12530_length_5267_cov_4.442249, whole genome shotgun sequence.
ACCESSION   DAOOJC010000128 DAOOJC010000000
VERSION     DAOOJC010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12530_length_5267_cov_4.442249"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            462..1097
                     /gene="yfbR"
                     /locus_tag="PLA01_04835"
     CDS             462..1097
                     /gene="yfbR"
                     /locus_tag="PLA01_04835"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HPD00660.1"
                     /translation="MEKQNNIETTQKNIQTDSSSCSHFAALLFRMKYINRWSLMRNTE
                     SENIAEHSLEVAIIAHLLATIKNKYYNGNLDPNYVAVLAMFHDASEIITGDMPTPIKY
                     FNPELNEAYKNVEKIANQKLLSMLPDDLKDTYRKILFHEGNEEWALVKAADKLAAYIK
                     CIEEEKAGNKEFLKAKESIFNSIKEMNRPEVKHFMDLFMCSFSLTLDELDY"
     gene            1593..4445
                     /locus_tag="PLA01_04840"
     CDS             1593..4445
                     /locus_tag="PLA01_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPD00661.1"
                     /translation="MRKKPLIIKKVQVILLIICMTASILSVPLNVTAETEVNFAKALQ
                     LSLYFYDANKCGEGITGGRLEWRGDCHVEDKEIPLIPMTEDYFGTNLSQSFIDEYRDV
                     LDPDGNGTLDLSGGMHDAGDHVKFGLPGTYAASTVGWGYYEFRDAYEKVGEKDHVEDI
                     LRWFNDFYLKATFLDDDGKVIAYCYQVGEGNIDHNYWNPPELQNSSYLLDFARPAYFA
                     TVETPASDQSAGAAASLAINYLNFKDEDPEYAQRCLDTAIALYDFARENRELGYSGGF
                     YGSSYDHDELAWAAVWLNIATGNMEYIDHIVETDSAGYYTGYMQRIIKDTGNDWQNIW
                     VHCWDTVWGGVFAKLAPITNTDRDWYIFRWNLEYWSGIPHENSKDTTFLAQSPAGYSV
                     VNSYGSARYNTAAQLCALVYRKYTGREDFADWAKGQMEYIIGNNPMDRSYIVGYSENS
                     AKHPHHRAAHGSKTYSMLDPEEHRHTLWGALVGGPDLDDNHVDETTDYVYNEVAVDYN
                     AAFVGASAGLYHYYGEELGHKPIENFPPKEEDVEEFFLEAKVEQENKERTQVTVKIYN
                     DTCHPPRFIRGMMTRYFFNISELLDAGQTIDDMTVEIYYDENAASYDGPATLRGPIKY
                     DDSGTYYVELDWTGRTIYGAREIQFALVSSQDSNYNSNWDPANDYSREGLGKEYIKTD
                     KIPLYVDGEKVCGNEPLKVEPSPTPTLDPHITPSPTPEETPSLEVSYKIGDTESAIKD
                     IKASIRIKNTGKKPVDLSNVKIRYWFTKDSATGQEFACDYAHIGKGLVTGTFVDLETP
                     VENADNYLEIGFDEDAGILGAGANTGEIQFRIIKGNFEAYDQSNDYSYMPDAIEFTAN
                     PCITAYVNSVLAYGISPVEEDTPDIVYGDLNGDDEVNSTDLTLLKRRLLRKIDKFDVP
                     DTHADLNIDGSIDSSDLTILKRYLLRKITELPVITN"
     gene            complement(4601..>5267)
                     /locus_tag="PLA01_04845"
     CDS             complement(4601..>5267)
                     /locus_tag="PLA01_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPD00662.1"
                     /translation="DKDGNITVEQGTNFISSIPLTELIKILNPKPPEEVLKAAEELKY
                     RSLLTVDIIVNKKVVFPDNWIYIHSPEVKLGRIQNFKNWSDKMVPNKEKTSLGLEYFC
                     DENDELWNMTDEELFKLASEEVEKIKICKASEIEDYVIVRVPKAYPVYNYGYKTYLKT
                     IVDYTKKFNNLQFIGRYGMFKYNNMDHSMLTGVNAAENIINPDLNIDIWQVNTEEEYH
                     EEK"
BASE COUNT         1680 a          994 c         1038 g         1555 t
ORIGIN      
        1 cttacactgt tcacttttca aagtccatat ccgcctgtct gaagcatttg actgctttca
       61 tcaaggcgac ttttatatac tatcatgctg tacttacttt gtcaacacct tttttaagat
      121 tttactgatg gtattttata gcactaacaa acctttataa ataatatagg atgaatatta
      181 tttaagtgag gaaaaataca caagaaacgt tgtgaagcaa gttacagccg cttgttaata
      241 cgacgtgaaa aaggagagca tccgtttcga atagtgagtt ttgctccaca ccacggagta
      301 ttaacagtca gccggactat taaaacttaa gtttttggaa ttcttttcac ttgtttgaca
      361 ccccccctgt aaatctgaaa tacctgtaca gtatatatat aaagtgttaa actatttaat
      421 gaaaacacaa ctataaaatt attctatatg gagagagtct tatggaaaag caaaacaaca
      481 tcgaaacaac gcaaaagaac attcagacag attcaagctc atgctcccat tttgctgctt
      541 tattattcag gatgaaatac ataaaccgat ggagcctcat gagaaataca gaatcggaaa
      601 atattgcaga gcacagcctt gaggttgcga taatagccca cttgctggca accatcaaga
      661 acaagtatta caatggaaac ttggacccga attacgtggc tgttcttgca atgttccacg
      721 atgccagtga aataatcacc ggtgatatgc ccacaccgat caagtatttt aatcccgagc
      781 ttaatgaggc ttataaaaat gttgagaaaa ttgcaaacca aaaacttctg tccatgcttc
      841 ccgatgattt gaaagacact taccgaaaaa tactgtttca tgagggaaat gaggaatggg
      901 ctctggtaaa agcagcagac aagctcgctg catacattaa atgcattgag gaagaaaaag
      961 cgggtaataa ggaattttta aaagcaaagg aatctatttt caattctatt aaagaaatga
     1021 acagaccgga agtaaagcat ttcatggatc tgtttatgtg cagcttttca ctgaccctcg
     1081 atgaattgga ctactgattt attgaacatc ggggtgtgta tattatttat gtaaaaaaat
     1141 atggaatgga catttcttaa catttattta atgtacccct ttctaataat aggcgggctc
     1201 tatatccggt gcgtttaaat aaaaaataat ttattatttt tcaattttat tctcaaacga
     1261 cccccaaaaa tttatttttc tttcgtatta atataatatt gatgtataat aaagacagaa
     1321 agcaaatttt tcgttgcagt aaatgttcta attctgatag aatgcaataa atcctaacag
     1381 accaagagaa aaattttctt aaccctatat aaatatttat tctatgcgtt atatacattt
     1441 ggattttgaa tacttacaaa ttgtattcag acaaatttaa tgctgtgata taaattttgc
     1501 gggaggtgaa agcagaaatt aaattcatat acagcatatg gaatgttata tacaagctat
     1561 attgtaattt ttgaggggag agtgaatgta ttatgagaaa aaagccactt attattaaga
     1621 aggtgcaggt tatccttctg ataatatgta tgactgcttc cattttatct gtaccgctta
     1681 atgtaactgc agaaactgaa gttaattttg caaaggcact gcaattgtct ctttatttct
     1741 atgatgccaa taaatgcggt gagggtataa cgggtggaag gcttgagtgg agaggcgact
     1801 gccatgtgga ggacaaggag attcctctta ttccaatgac agaagattat tttgggacaa
     1861 acctttcaca aagctttatc gatgagtaca gagacgtatt ggatcctgac ggaaacggca
     1921 ccctggacct gagcggcgga atgcatgatg caggtgacca tgttaaattt ggtcttcccg
     1981 gcacctatgc agcttcaact gttggctggg gatactatga gtttagagat gcttatgaga
     2041 aagtcggaga aaaagaccat gtagaggata tattaagatg gtttaacgat ttttatctga
     2101 aggcaacatt tttagatgat gacggaaaag ttatcgctta ctgttaccag gtgggagagg
     2161 gaaatataga ccacaactac tggaatcccc cagagcttca aaattcctct tatcttctgg
     2221 actttgcaag acctgcgtat tttgccacag tagaaacacc tgcaagtgac cagtctgcag
     2281 gtgctgcagc atctcttgct atcaattatc tcaactttaa ggatgaagat cccgaatatg
     2341 cacagaggtg ccttgataca gctattgccc tttatgattt tgccagagaa aacagagagc
     2401 tgggctattc cggtggtttt tacggttcca gttatgacca cgatgagttg gcctgggcag
     2461 ctgtctggct caatatagca acaggaaaca tggaatatat tgatcatatt gtggaaaccg
     2521 acagtgctgg ttattacacc ggttatatgc agagaattat taaggacaca ggcaacgact
     2581 ggcaaaatat ttgggttcat tgctgggata ccgtttgggg aggagtattt gcaaagcttg
     2641 ctcctataac caacaccgat agagactggt acatattccg ctggaacctt gaatattggt
     2701 ccggtatacc tcatgagaat tccaaagaca ccactttcct tgcacaaagt cccgcaggct
     2761 acagcgtagt taactcatac ggttctgcaa ggtataacac ggcagcacag ctctgtgccc
     2821 ttgtttacag gaaatatacc ggcagggaag attttgcaga ttgggccaaa ggccaaatgg
     2881 aatatatcat aggcaataac cctatggata gaagctatat tgtaggatat tcggaaaact
     2941 ctgcaaagca tcctcatcac agagcagctc acggttctaa gacctatagc atgctggatc
     3001 ctgaagaaca ccgccatact ctctggggag ctttggtagg aggtcctgac ctcgatgaca
     3061 accatgtgga tgaaaccacc gattatgtgt ataacgaagt ggccgttgac tacaacgccg
     3121 cttttgtagg tgcctctgcc gggctttacc attattacgg cgaagaactc ggacacaaac
     3181 ccattgaaaa cttccctcca aaggaagaag atgttgaaga atttttcctg gaagcaaagg
     3241 ttgaacagga aaacaaggaa agaacccagg taacggtaaa aatttataac gatacctgtc
     3301 atccgccaag atttataaga ggaatgatga cccgttactt cttcaatatt tccgagctgc
     3361 tagatgccgg tcaaacgatt gacgacatga cggttgaaat atattatgat gaaaatgcgg
     3421 caagttatga cggtccggca acattaagag gaccaattaa atacgatgac agcggtacat
     3481 attatgtgga acttgattgg acaggccgca ccatatatgg agcccgcgaa attcagtttg
     3541 cgttggtttc cagccaggat tcaaattaca acagcaattg ggatcccgca aacgattaca
     3601 gcagagaagg attgggaaaa gagtacatca agacggataa aatacctcta tacgttgatg
     3661 gagaaaaagt ttgtgggaat gagcctttga aggtagagcc ttctcccaca cctactcttg
     3721 atccgcatat tacaccatcg cctactccgg aagaaacacc ttcccttgag gtctcgtaca
     3781 agatcgggga cacagaatcg gcaataaaag acatcaaggc ttccatcagg attaaaaata
     3841 cgggaaagaa gccggtagat ctgtcaaatg taaagatacg ctactggttt accaaggact
     3901 cagccacagg tcaagagttt gcctgtgatt atgctcatat aggtaaaggc ttggtaacag
     3961 gtacatttgt tgatcttgaa acccctgttg aaaatgcaga taattacctt gagattggtt
     4021 ttgacgaaga tgcaggtatt ttgggagcgg gtgccaatac cggcgaaatt cagttcagaa
     4081 tcattaaggg caactttgaa gcctacgatc aatcaaatga ttactcatac atgcccgatg
     4141 caatagaatt cacagcaaat ccttgcataa ctgcatatgt taattcggtt ctggcatacg
     4201 gtatttcgcc ggttgaagaa gatacacccg atattgtata cggagatctc aacggtgatg
     4261 atgaagtaaa ctcgacagat ttaaccctat taaagagacg tctgttaaga aaaatagata
     4321 aattcgatgt gcctgacact catgcagatt tgaacatcga cggcagtata gactcatctg
     4381 atttgaccat cttgaagaga tatcttttaa ggaaaataac cgaattacct gttataacga
     4441 attgacaatt ttgattatcc tctatttcag gctttagtga agcatttaaa ttgcttcgct
     4501 aaagccttat aaaattaaca agtctccctt catggttgat ctcggtatcg tccacaaagg
     4561 gagactttta tgataatgtt attcttcttt atcttctata ctatttctct tcgtgatatt
     4621 cttcctccgt attcacctgc caaatatcaa tgttcaaatc cggatttatg atattctctg
     4681 ctgcgttaac cccagtcaac attgaatggt ccatgttatt gtatttaaac atcccatatc
     4741 ttccaatgaa ctgtaagtta ttgaattttt ttgtataatc aactatagtt ttaagataag
     4801 tcttgtagcc ataattatag acaggatatg cctttggtac ccgcacaata acataatctt
     4861 caatttcaga tgctttacat attttaattt tttcaacctc ttcagatgca agcttaaaca
     4921 actcttcatc agtcatattc cataattcat cattctcatc gcaaaaatac tcaagaccta
     4981 aagaagtctt ttccttattc ggaaccattt tatcgctcca gttcttaaaa ttctgaatcc
     5041 ttccaagttt tacttcagga gaatgtatgt aaatccaatt atcaggaaaa actacttttt
     5101 tgttaacaat tatgtcgaca gttaaaagac ttctgtattt tagttcttcg gctgccttta
     5161 aaacctcttc aggcggcttt ggatttaaaa tttttatcaa ttcagttaat ggaatactgg
     5221 atataaaatt agttccttgt tcaactgtaa tattgccgtc tttatcg
//
LOCUS       DAOOJC010000129         5267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12531_length_5267_cov_3.358979, whole genome shotgun sequence.
ACCESSION   DAOOJC010000129 DAOOJC010000000
VERSION     DAOOJC010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12531_length_5267_cov_3.358979"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLA01_04850"
     CDS             <1..382
                     /locus_tag="PLA01_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HPD00663.1"
                     /translation="DKRTNIIGLAHAMLPDSLQINNRSNTAKFVDTAVDALIIEMEKL
                     GAVKSAIVAKLAGGAQMFAFSQSSDLMRIGYRNVVAAKEKLRQLGIPLVAEDTGGNYG
                     RTIELDSATGILLVKTIGYGTKRI"
     gene            398..808
                     /locus_tag="PLA01_04855"
     CDS             398..808
                     /locus_tag="PLA01_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00664.1"
                     /translation="MDYTSKIPFISGASVTIIVGILSYKSGYELKTICIRMTFSMVVF
                     FLIGIYLRSFIRRMKREVKQKKVMEEIKKNRELLVEAAKKNAQRKNSKINLTVGNDTQ
                     EDIHADEENLYDEEFKPLKVSNVTIADQDENSQQ"
     gene            878..1648
                     /locus_tag="PLA01_04860"
     CDS             878..1648
                     /locus_tag="PLA01_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HPD00665.1"
                     /translation="MSSANAKQLELWKQYSDTKSPAIKERLIVEYSNIVKYIAGRLSI
                     YFGSNVEYDDLIGYGIFGLIDAIEKFDLKKGVKFETYASLRIRGAIIDSIRELDWVPR
                     SLRQKNKELERAYSELENELGHSATDKEVAERLGISIQELNKLLGEVNLSSMVSLEEF
                     LEQNYEIGVSNTPGSVDEKPEGYLEMAELKEILVDAIGKLPEKEKMVVSLYYFEDLTL
                     KEISAIMKVSESRISQLHTKAILRLRGKLARHKSILRD"
     gene            1684..3111
                     /locus_tag="PLA01_04865"
     CDS             1684..3111
                     /locus_tag="PLA01_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPD00666.1"
                     /translation="MDNSGNLFEEKMKMERNIDSLISLEVSSDKMKASIIVTPPQDGR
                     NLSQKEIVEFLKRKGVVYGIIEPNIDYISKYSVYNERIEIAEGTPPTSGHNGRVEFHF
                     DLKREKRPVILDDGRVDFRNLNLVQTVKKDDVLCSLVPPSPGTPGKTVLNTEIPAING
                     KPAMLPTGKNVVVSEDGQSLIASIDGQVSYIDGKVSVFANYEVSGDVDNSTGNINFIG
                     NVIVRGNVLSGFSIEAGGNVEVMGVVECAFIKAGGDIILRRGMQGLGKGVLISGGDIV
                     ARYIENSNIQAKRDIKSEAIMHSNIICGNKLELSGKKGLLVGGNCKVGKEIVAKVIGS
                     YMNTPTEIEVGVDPSIKDHYKKVRDEMRQMEEDIKKAEQAIAMLKRMEMNGNLSPEKQ
                     EIMAKSVRTKIHFSNRIEELKGELIQMELKLQQEANGKVRAYDYIYPGTRVTIGTSSL
                     YVKENLQYCSLYRDGVDIKIGSIDK"
     gene            3223..3534
                     /locus_tag="PLA01_04870"
     CDS             3223..3534
                     /locus_tag="PLA01_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00667.1"
                     /translation="MSIKPIDFQVMIPKTAEVSKIQKDEQNKNVALEQQQTIVNKKKS
                     EDNVNLVHSQDETQNAKIDDEQERKQKENKQKKNRFKGRYGTKGERETDEGTGTIDIR
                     L"
     gene            3620..4195
                     /locus_tag="PLA01_04875"
     CDS             3620..4195
                     /locus_tag="PLA01_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00668.1"
                     /translation="MTGFYASIMFIGILLVLVSLVWIAYDKKKYLEDEKRMDEKKEEL
                     LKIISDADLMIDELNRISDYVVSEMDKKSKEVQNAIESMSEKVKSISNEKVKSISNEK
                     EKNKSKENNALIKDKTLGTGRLSKEVGYVFNAEPSIDKSYKKLNDKVIAVNSKHKEVL
                     ALAQRGLNETEIAKKLSMGKGEIQLILGVNK"
     gene            4271..4795
                     /locus_tag="PLA01_04880"
     CDS             4271..4795
                     /locus_tag="PLA01_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HPD00669.1"
                     /translation="MKSFYIKSVLLGIGIGIIFTSLVGIIYSAGMSPQMSKLEIMEKA
                     KQYGMVLGSDTIIAADDNKTDGTALEEEEPVPGYNNAVDTPKPIETEGLRENTEKPTD
                     EPAQEVLIKIDPGDTSKVVAEKLYEKGLIDSVDSFENFLKEKNLHGSIQVGDFVIAKG
                     TDKATIARIISEIR"
     gene            5013..>5267
                     /locus_tag="PLA01_04885"
     CDS             5013..>5267
                     /locus_tag="PLA01_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00670.1"
                     /translation="MKAYLITLIALLQTFLFSACTVSMVEPGPKNEVNSYIERVIKAD
                     KPLVLDLFCDSGNIEVYTWEKSEIKFEVTKRVRGVLKKEIL"
BASE COUNT         1882 a          687 c         1283 g         1415 t
ORIGIN      
        1 cgataagagg acaaatataa ttggattggc tcatgctatg ctgccggaca gtttacaaat
       61 aaataacagg tccaacactg ctaagtttgt agatactgct gttgatgcac ttattattga
      121 aatggaaaaa ctgggtgcag tcaaaagtgc aattgtagca aagcttgcag gaggggcaca
      181 gatgtttgcc ttttcccaat cttcggattt aatgaggata ggttacagaa atgtggttgc
      241 ggcaaaggaa aaactgagac aattaggtat tccattggtt gctgaggata ctggaggtaa
      301 ttatggcagg actattgaat tggattcagc cacaggcatt cttctcgtga agacgattgg
      361 atacggaact aagagaatat aaagagagga caaaaggatg gattatacca gcaaaatacc
      421 gtttattagt ggtgcttccg ttacaataat agtaggaata ttgagttata aaagcggcta
      481 tgaattaaaa acaatttgca taagaatgac attcagcatg gtggtatttt ttctaatcgg
      541 tatatacttg agaagcttta ttagaagaat gaaacgggaa gtaaaacaga aaaaggtaat
      601 ggaagaaata aaaaaaaatc gcgagctttt ggttgaagca gcgaagaaaa atgcacaaag
      661 aaaaaacagt aagataaatt tgacagtggg aaatgacact caagaggata tacatgccga
      721 tgaggaaaac ctttatgacg aagagtttaa gccgcttaaa gtgagcaatg ttactatagc
      781 ggatcaggat gaaaattcac aacagtaaat atcgcaattt ttctaagcag tttattagct
      841 tatatatatg aaaataaatg ggtaatgggg gattaatatg tcatctgcta acgctaaaca
      901 attggaatta tggaaacaat acagtgatac gaaaagccct gcaatcaagg aaagacttat
      961 tgttgagtat tccaatatcg tcaaatacat agcgggaagg cttagcatat attttggatc
     1021 caatgttgag tatgatgacc tcataggata tgggattttt ggtctgattg atgctattga
     1081 aaagtttgac ttgaaaaaag gtgtaaagtt tgaaacctat gcatcgttaa ggataagagg
     1141 ggcgattatc gatagtatcc gggaactgga ttgggtacca aggtccttaa gacagaagaa
     1201 taaagaactt gagagggctt attcagaatt ggagaatgaa ctcggacatt cggcaacgga
     1261 taaggaagtg gccgaaagac tgggcataag cattcaagag cttaacaagc tattgggaga
     1321 agttaattta tcctcgatgg tttctcttga agagtttctg gagcagaatt acgaaatagg
     1381 tgtttccaat acacccggaa gtgttgacga aaagcctgaa gggtatttgg aaatggcgga
     1441 gcttaaggaa atacttgtag atgcgatagg taagcttcct gagaaggaaa aaatggtggt
     1501 ttcgctgtat tattttgagg atttgacact aaaggagatc agtgcaatta tgaaagtatc
     1561 ggaatcacga atatcacagc ttcacaccaa agctattttg cggcttaggg gtaagcttgc
     1621 acgccataaa agcatcttga gggattgaca gtaatatatt gaattgaaag gaggggtagg
     1681 tttatggata attcgggtaa tctatttgaa gaaaagatga agatggaaag gaatattgat
     1741 agtttgattt ctttagaggt gtcaagcgac aagatgaaag catccattat tgtgacacct
     1801 ccacaggacg gaagaaattt aagccagaag gaaattgttg aatttttaaa aagaaaagga
     1861 gtggtctatg ggattattga gcctaatatt gattatattt caaagtattc tgtttataat
     1921 gagaggattg aaatagcaga gggtactcct cccacaagcg gtcacaatgg aagagttgag
     1981 ttccattttg atttgaagag ggaaaagaga ccggtaattt tagatgacgg aagggttgat
     2041 tttagaaacc tgaatcttgt acagactgta aaaaaggatg atgttttatg ctctcttgtg
     2101 ccaccctctc caggaactcc cggaaaaacc gtcttgaata cagaaatacc tgcgataaac
     2161 ggtaagcctg caatgttgcc gacggggaaa aatgtagtag tatccgaaga cgggcaaagt
     2221 cttattgcca gcatagatgg acaagttagc tatatagacg gcaaagtcag tgtttttgcc
     2281 aactacgaag tttccgggga tgtagataac tctacgggaa atattaattt tatcggtaat
     2341 gttattgtac ggggaaatgt gctctcgggt ttttcaattg aagccggggg taatgttgag
     2401 gttatgggtg ttgtggagtg tgcttttatt aaggctggtg gagacattat attgagacgg
     2461 ggaatgcagg gacttgggaa aggtgttttg attagtggcg gagacatagt tgcaagatat
     2521 attgaaaaca gcaatattca agcaaaaaga gatataaagt cggaagctat tatgcacagc
     2581 aatataatat gcgggaacaa gctggaactg tccggaaaga aaggtcttct tgtcggaggc
     2641 aactgtaagg ttggaaaaga gattgttgcg aaggttattg gttcatatat gaatacacca
     2701 acggaaattg aagtaggtgt ggatccttct ataaaagacc attacaaaaa agtacgggat
     2761 gaaatgcggc agatggagga agatattaaa aaggccgagc aagccatagc aatgctgaaa
     2821 aggatggaaa tgaacggtaa tttatctccg gaaaagcagg agataatggc aaaaagcgtt
     2881 aggaccaaga ttcatttttc caacaggatc gaggagttaa aaggagaatt aattcagatg
     2941 gagcttaagc ttcagcagga agcaaacgga aaggtaaggg catacgatta tatatatccg
     3001 ggaacgaggg tgactatagg tacgagttcg ttgtatgtca aagagaacct gcagtattgt
     3061 tctttataca gggatggtgt agatatcaag attggatcca ttgacaagtg atttatggaa
     3121 tgtttaaaag ctggatttca aagagtggga ggatcaaaaa accatcaaaa cgaggttatc
     3181 ttggtattat aattagaaag aataatgttt ggaagtgatg ttatgtcaat taaacccatt
     3241 gattttcaag tgatgattcc taagacagca gaggtatcaa aaattcagaa ggatgaacaa
     3301 aataaaaacg tcgctcttga acagcagcaa accatagtaa ataagaagaa atccgaggat
     3361 aatgtcaatc tggttcattc tcaggatgaa actcaaaatg cgaaaataga tgatgaacaa
     3421 gaaagaaagc agaaggaaaa taaacaaaaa aagaacaggt ttaaagggag gtatggaaca
     3481 aaaggagaac gtgaaacgga tgaagggaca ggcacaatag acatacgtct atagcaaaga
     3541 taaattctta tttagcaacg ggtagatata taaaaaagca gggagtttta ctattataga
     3601 taattatgga gtgatgaaaa tgacgggttt ttatgcaagt atcatgttta taggcatatt
     3661 gttggtactg gtttctctgg tatggattgc ttatgacaaa aagaagtact tggaagatga
     3721 gaaaagaatg gacgagaaga aagaagagct cttaaaaatc atttccgatg cagatcttat
     3781 gattgatgaa cttaacagga tttcagatta cgttgtttct gagatggata aaaagagcaa
     3841 ggaagtgcag aatgcgattg aaagcatgag tgagaaagta aagagtatat caaatgagaa
     3901 agtaaagagt atatcaaatg agaaagaaaa gaataaatca aaagaaaaca atgctttgat
     3961 aaaggataaa acattaggga ccggaagatt atcgaaagaa gtgggctatg tttttaatgc
     4021 tgaaccctct atagataaat cttataagaa gcttaacgat aaggtaattg ctgtgaacag
     4081 caagcataaa gaggttttag ccctggctca aagagggttg aatgaaactg aaattgcaaa
     4141 gaagttaagt atgggaaagg gtgaaataca gctcatactt ggtgtaaata aataatgctt
     4201 gctctatatg aaataatcat cttttttgat aatactatag gttatcacta ttatttattg
     4261 gagggagctt atgaagagtt tttatataaa aagtgttttg cttgggattg gaataggtat
     4321 tatttttaca tcattagtcg gtattatata ttctgcagga atgagccctc aaatgagcaa
     4381 gctggagata atggagaagg caaagcaata cggtatggta ttgggcagtg acacaataat
     4441 cgcggcagat gacaacaaaa cggacggtac agctttagag gaggaagaac ctgtacctgg
     4501 atataataac gctgttgata ctccaaagcc aatagaaact gagggtttaa gagaaaatac
     4561 ggaaaaaccc acggatgagc cggcacagga ggttttaatc aagatagacc caggggatac
     4621 atcaaaggta gtggctgaaa aactctatga aaaagggttg atagatagtg ttgattcctt
     4681 tgagaatttt cttaaagaaa agaaccttca tggatccata caggtgggag attttgtaat
     4741 agcgaaaggt accgataaag ctactatagc aagaattatt agcgaaattc gttagtatat
     4801 agtttaaagg gtttaacccc ggttcttttg accgtttttt aggacaggtt aaaagaatcg
     4861 gggttatttt attagtggga tttcacaaag tgaaatgacc ctcgatgcca tcagagcttt
     4921 tgcttagtca ggaaatattt gtagaaagtt ttaacactat gttattattt atcaatacag
     4981 agaaataata ctgatagaaa gaggtgaagg gtttgaaagc ttatcttatc acacttatag
     5041 ctttattgca gacatttttg ttttctgcct gcacggtttc aatggttgaa ccgggaccta
     5101 aaaatgaggt gaattcttat attgaaaggg tgattaaagc ggataagccc cttgttctcg
     5161 accttttctg cgattccgga aatatagagg tttacacctg ggaaaaatcc gagattaaat
     5221 ttgaagtaac aaagagggta aggggagttc tgaaaaaaga aatttta
//
LOCUS       DAOOJC010000130         5236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12632_length_5236_cov_3.515538, whole genome shotgun sequence.
ACCESSION   DAOOJC010000130 DAOOJC010000000
VERSION     DAOOJC010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12632_length_5236_cov_3.515538"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..614)
                     /gene="rplT"
                     /locus_tag="PLA01_04890"
     CDS             complement(261..614)
                     /gene="rplT"
                     /locus_tag="PLA01_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPD00671.1"
                     /translation="MSRVKGAVRTRARHKKIIKLAKGYFGAKSTNFRIANQAVMKSLV
                     YAYRDRRAKKRDFRQLWITRINAAARLNGLSYSRFMNGLKKTGITLNRKMLAEMAVND
                     EKAFAQLVEKVKSAK"
     gene            complement(630..827)
                     /gene="rpmI"
                     /locus_tag="PLA01_04895"
     CDS             complement(630..827)
                     /gene="rpmI"
                     /locus_tag="PLA01_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPD00672.1"
                     /translation="MPKIKTHSSSKKRFNLTATGKVKRSKAYKSHILTKKTTKRKRNL
                     RKSTIASDANASVIKVLIPYK"
     gene            complement(852..1346)
                     /gene="infC"
                     /locus_tag="PLA01_04900"
     CDS             complement(852..1346)
                     /gene="infC"
                     /locus_tag="PLA01_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPD00673.1"
                     /translation="MVNEQIRDKEVRVIDADGSMLEIMSSKDAQKLANSKNLDLVKIA
                     PQAVPPVCRIMDYGKYMFDLAKKEKEAKKKQRVINIKEVRLSSSIEDHDFNFKLKNAV
                     KFLKDGDKVKVSIKFRGREMNYTSLGQQILEKFAVAIEEFAVVERKPKLEGRNMFMIL
                     NPKQ"
     misc_feature    complement(1464..1599)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(1657..2202)
                     /locus_tag="PLA01_04905"
     CDS             complement(1657..2202)
                     /locus_tag="PLA01_04905"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPD00674.1"
                     /translation="MKAIVAVDQNWGIGYKGSLLQRIPEDMKFFKQMTLGKVVVMGRE
                     TFESLPGREPLKDRTNIVLSKNKNFANENLILCRSFEELTQELRKYNFDDVFVIGGES
                     VYTQLLPYCTEAYVTKINKSYIADKYFCNLDEEQNWEMVSMSDLRIYNDIQYKFTKYL
                     NGKAVDFFEPKDSDVNRKILM"
     gene            complement(2216..3046)
                     /gene="thyA"
                     /locus_tag="PLA01_04910"
     CDS             complement(2216..3046)
                     /gene="thyA"
                     /locus_tag="PLA01_04910"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPD00675.1"
                     /translation="MSKADDIFIAMCKDILENGISSEGEIVRAKWDDGTFAHTIKKFA
                     VVNRYDLSEEFPILTLRPTNLKAAIDELLWIWQKKSNNIKDLNSRIWDSWADEQGSIG
                     KAYGYQLGIKHKYREGEFDQVDRIIYDLKHNPYSRRIIANMYNHSDLQEMNLYPCAYS
                     ITFNVTGSKLNAILNQRSQDVLVANNWNVAQYAILVHMFAQVSNLEVGELVHVIADAH
                     IYDRHVPLITELIQRTPYPAPKLLINPDIKNFYDFKVEDFVLEGYQTGAQIKSIPVAV
                     "
     gene            complement(3162..5069)
                     /gene="thrS"
                     /locus_tag="PLA01_04915"
     CDS             complement(3162..5069)
                     /gene="thrS"
                     /locus_tag="PLA01_04915"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPD00676.1"
                     /translation="MIKVTLKDGSVKEYNKGVTIKSVAESISAGLARVALAGEVNGEV
                     KDLDYQLEEDCSLNLLTFDDEGGRHAYRHTSSHIMAQAVKRLYPEAKLAIGPSIENGF
                     YYDFDVEKPFSVEDLESIENEMNKIIKEDYKLERFTLPRDEAIKFMEEKQEPYKVELI
                     RDLPEGETISFYKQGDFVDLCAGPHVESTGKIKAYKLLSVAGAYWRGNEKNRMLQRIY
                     GTSFNKKSELDAYLTRLEEAKKRDHRKLGKELDLFDILEEGPGFPFFMPKGMVLRNTL
                     EEYWREEHKKAGYQEIRTPIILNEELWHRSGHWDHYKENMYFTKIDEGDFAIKPMNCP
                     GGMLVYKRKLHSYRDLPQRLAELGLVHRHELSGALHGLMRVRCFTQDDAHIFMTPEMI
                     KDEVVGVINLIDDFYKVFGFKYHIELSTRPENSMGSDEQWEQATNALKNALEARGLSY
                     KINEGDGAFYGPKIDFHLEDSIGRTWQCGTIQLDFQMPERFDLTYVGADGEKHRPVMI
                     HRVVFGSIERFIAILTEHYAGAFPVWLSPVQVRILPIVDKHHDYAEEIKTQLEKEGIR
                     VELDLRNEKIGYKIREAQLAKVPYMLVIGDKETETKTLAVRSRKEGDLGTMSPADFIA
                     RIINEIKNKEN"
BASE COUNT         1419 a         1241 c          778 g         1798 t
ORIGIN      
        1 ttaatagaga tattttcttt taaggcctcc tcgacaaacc ggataccctc aataaaaaac
       61 ataccgcttt cctcacggaa ttttttctgt tttaatgctt tgacttcttt aataaccgga
      121 ttttgagcac tgcttatgta tttcatgcac atctcctcaa aataaatttc gttgttattc
      181 tttggataaa taaataatac gtcttcttaa atcacaaaaa agagagagct cttttgagtt
      241 cccccttttt tatgaaaaaa ttattttgcc gacttaactt tttccactaa ctgagcaaaa
      301 gccttttcgt cattaacagc catctctgcg agcatttttc tgttaagagt tataccagtc
      361 ttcttaagcc cattcataaa tctgctatag ctcagaccgt tcaatcttgc tgcagcattt
      421 attctggtaa tccagagctg cctgaaatct cttttcttag cccttctatc cctgtaagcg
      481 taaaccaatg atttcattac agcctggttt gcaattctaa agtttgtact tttagctccg
      541 aaataaccct ttgccagctt tattattttc ttatgtcttg cacgcgttct taccgcacct
      601 ttaactcttg acatagccac actcctcctt tacttgtacg gaatcaacac tttgatcact
      661 gaagcattag catcggatgc aattgttgat ttcctcaagt ttcttttcct tttagtagtt
      721 ttctttgtca atatgtgact tttgtaggct tttgatcttt ttaccttacc tgttgctgtt
      781 aaattaaatc tctttttgga agagctgtgt gttttaatct tcggcataag atttcctcct
      841 taaattctat attactgctt aggattaaga atcataaaca tgttcctacc ttcaagctta
      901 ggttttcttt ccactactgc aaattcttca attgcaaccg caaatttctc aagtatctgt
      961 tgacctaacg aggtgtaatt catttctctt cctctaaact taatactaac tttaacctta
     1021 tcaccgtctt ttaaaaactt gacggcattt ttcaacttaa agttgaagtc atggtcttca
     1081 attgatgaag atagtctgac ctcttttata ttaatgaccc tctgtttctt ttttgcttct
     1141 ttttcctttt tggcaaggtc aaacatatac ttgccatagt ccataattct acatactgga
     1201 ggcactgctt gcggtgcaat cttaaccaaa tcaagatttt tggaatttgc aagcttctgt
     1261 gcatccttag aagacattat ttcaagcatg gaaccatcag catcaatcac ccttacttct
     1321 ttgtccctta tttgctcatt gaccattaat tcgtttttgt taataaaaaa cacctccggg
     1381 ttttttgaga ttttgcgtaa tttttaaaag ctttccgggc attcggccca tttacttaca
     1441 gcaagatttc gattcatgaa atcacgcata taaaaaggtg gacccagaaa tgatccacct
     1501 atctctcaac acataattaa caacatctgt agcttaagag tattaacctt gcaaaaacaa
     1561 gtccgcaagg tgagaagtgg attacttctg ctttcttttc gcttattcat aatatcaaat
     1621 gctttagtat ttgtcaagaa tttttattaa ataattttac atcagaatct ttcggtttac
     1681 atcagaatct ttcggttcaa aaaaatcaac cgctttgccg ttcaaatact tagtaaactt
     1741 atattgaata tcattatata tccttaaatc actcatcgac accatttccc agttttgttc
     1801 ctcatccagg ttacaaaaat acttgtctgc aatataagat ttattgattt ttgtaacata
     1861 agcctctgta caatacggca aaagctgcgt atataccgac tccccgccta ttacaaaaac
     1921 atcgtcaaaa ttatacttcc taagttcctg agtcagttct tcaaatgagc ggcacaaaat
     1981 tagattttca tttgcaaaat tcttattctt gctcagtaca atattagttc tatccttaag
     2041 aggctctctt cccggtaacg attcaaaggt ttctctgccc atcaccacta ctttgcctaa
     2101 agtcatctgc ttgaaaaact tcatatcttc gggtattctt tgcagtaagc tgcctttata
     2161 accgattccc caattttgat caactgcaac aatagctttc atcttattct cctttttaaa
     2221 cagctacagg aatactcttt atctgtgctc cggtctgata gccctccagt acaaagtcct
     2281 caactttaaa atcataaaaa ttctttatat caggattaat cagcagcttt ggtgccggat
     2341 aaggagtcct ctgtataagc tctgtaatta acggcacatg cctgtcatat atatgtgcat
     2401 ccgcaattac atgtacaagc tccccaacct caagattgct tacctgtgca aacatatgca
     2461 ctagaattgc atactgagcc acattccaat tatttgcaac caagacatct tgagaacgct
     2521 ggtttaaaat agcatttagt ttactgccgg taacattaaa agttatacta tatgcacaag
     2581 gatacaagtt catctcttgc aagtcactat ggttgtacat gttagcaata atccttctgc
     2641 tgtatggatt atgctttaaa tcatagataa tccggtctac ctggtcaaac tctccttcac
     2701 ggtacttgtg cttaattccc aattgataac cgtatgcctt gccgattgaa ccttgttcat
     2761 cagcccaact gtcccaaatg cggctattaa ggtcttttat attgtttgat tttttttgcc
     2821 atatccaaag caattcatca atagcagctt ttagatttgt cggcctcaaa gtaaggattg
     2881 gaaattcctc agacaaatca tatctgttaa caactgcgaa tttcttaatt gtatgggcga
     2941 aggttccgtc atcccactta gctctgacga tctcaccttc agaagatatt ccgttttcga
     3001 gtatatcctt acacattgca ataaaaatat catccgcttt actcatatta acttacctca
     3061 cttatcaccc attgtttatc aatagtttat attaagcaca tatatcactc tttgccatta
     3121 aacctgcctg atctgttgaa aacctaagtt gcgtattttt attagttttc cttattctta
     3181 atctcattaa taatccttgc aataaaatct gccggtgaca tagtgcccaa gtccccttcc
     3241 tttctggacc ttactgccaa ggttttagtc tcggtctcct tatcacctat tacaagcatg
     3301 taaggtacct ttgccagttg tgcctctctt attttatagc caatcttctc gttcctcaaa
     3361 tccagctcta ccctgattcc ttctttttct aactgtgtct tgatttcttc ggcataatca
     3421 tggtgtttat caactatggg cagtattcta acctgaaccg gtgaaagcca taccggaaaa
     3481 gcacctgcat agtgctctgt caaaatggca ataaaccttt ctatgctgcc gaaaacaacc
     3541 ctgtgaatca taacaggcct gtgcttttct ccatcagcgc cgacatatgt caaatcgaat
     3601 ctctccggca tctggaaatc caactgtatt gttccgcact gccaggtacg tccaatggaa
     3661 tcctccagat ggaaatctat cttcggcccg taaaaagccc cgtctccttc atttatcttg
     3721 tagctgaggc ctcttgcttc aagggcattc ttcaatgcat tggttgcttg ctcccactgt
     3781 tcgtcggaac ccattgaatt ttcaggtctt gttgaaagtt caatgtggta tttgaaaccg
     3841 aataccttat aaaagtcatc tataagattt attactccaa ctacctcatc cttaatcatt
     3901 tccggagtca taaaaatatg ggcatcatcc tgggtaaagc atctaaccct catcaagcca
     3961 tgtaaagctc cggaaagctc atgtctgtgc accagtccaa gctctgccag cctctggggt
     4021 aaatcccggt aagagtgaag ctttctttta taaacaagca taccaccagg gcagttcatc
     4081 ggtttaatag caaaatctcc ctcatctatc ttggtaaagt acatgttttc cttgtagtgg
     4141 tcccaatgtc ccgaacggtg ccagagctct tcatttaaaa taatcggagt ccttatttcc
     4201 tgataccctg cctttttgtg ctcttctctc cagtactcct ccaaggtatt tctcaaaacc
     4261 atacctttgg gcatgaagaa cggaaaaccg ggtccctctt ctaatatatc aaaaagatcc
     4321 agctcttttc cgagcttccg gtggtctctc ttctttgcct cctccagcct tgtaaggtaa
     4381 gcatccagtt cacttttttt attgaaggat gtaccataaa ttctttgaag catcctgttt
     4441 ttttcatttc ccctccaata agcaccggct accgataaca gcttgtatgc tttaatcttg
     4501 ccagtagatt ccacgtgagg tccggcacac aaatccacaa agtctccctg tttgtaaaaa
     4561 gatattgttt caccctccgg caaatcccga ataagctcca ccttgtatgg ttcctgtttc
     4621 tcttccataa atttgattgc ttcatccctc ggcagagtaa acctctcaag cttatagtct
     4681 tcctttatta ttttgttcat ttcattttcg atgctttcca aatcctcaac cgaaaaaggc
     4741 ttttccacat caaagtcata ataaaaaccg ttttctattg aaggccctat tgcaagcttt
     4801 gcttccgggt ataatctctt aacagcctgt gccattatat gagatgacgt atgcctgtat
     4861 gcatgccttc cgccttcgtc atcaaaggtc aaaaggttta aagagcagtc ctcctcaagc
     4921 tgataatcca gatccttcac ctcaccgttt acctctccgg caagagccac ccttgccaat
     4981 cctgcactta tgctttccgc cacactttta atggtgacac ctttattata ttccttaaca
     5041 ctaccgtcct ttaatgtaac tttaatcata gatatgcctc ctgtttataa taattctcga
     5101 aagatatttt atgggaatca ataaaaaaac ccccatcccc tgaaagggac gagagttatc
     5161 tcccgtggtt ccaccctaat tgtttactca ttatgactat aacgtaatca ccgtttcaac
     5221 tccgaggcgg tcttca
//
LOCUS       DAOOJC010000131         5229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12652_length_5229_cov_3.265559, whole genome shotgun sequence.
ACCESSION   DAOOJC010000131 DAOOJC010000000
VERSION     DAOOJC010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12652_length_5229_cov_3.265559"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1971)
                     /gene="aspS"
                     /locus_tag="PLA01_04920"
     CDS             complement(190..1971)
                     /gene="aspS"
                     /locus_tag="PLA01_04920"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPD00677.1"
                     /translation="MGESIRGLKRTHMCAGLGINDVGKNVTVMGWCHRTRNLGGVIFI
                     NIRDRSGIIQTVYNDESDPEIFEKASTVRGEYVVAVSGVVSNRAPEAINPEMATGEIE
                     IIAKELRILSSAETPPMYIEEDSDVNEATRLKYRYIDLRRPDMQRNLMLRHRVSKIAR
                     DYYDEKGFLEIETPMLTKSTPEGARDYLIPSRVHPGKFFALPQSPQLFKQLLMVSGFD
                     RYFQIVKCFRDEDLRADRQPEFTQIDLEMSFVNVDDVLSVNEEFIKRLFKEAINVDIE
                     IPLLRMPYKEAMERFGSDKPDIRFGLELVNVSDLVENCGFKVFSDAVNSDGSVRAINA
                     KGCGEKFKRKEIDALVELVKTYKAKGMAWIVVEENGLKSSITKFFSEDEMNAILQRVE
                     AQPGDLICFVADKDEVVFDSLGQLRLEIARKLGLTDSKEFKLLWVTEFPLLEYDEEEK
                     RYVAKHHPFTSPMDEDIELLDTDPGKVRAKAYDMVLNGNEIGGGSIRIHSQELQSKMF
                     KLLGFSDEDAWARFGFLLEAFKYGTPPHGGMAFGLDRLVMLMAGRSSIRDVIAFPKVQ
                     NASCLMSNAPDDVDPEQIKELHIKLDL"
     gene            complement(1995..3248)
                     /gene="hisS"
                     /locus_tag="PLA01_04925"
     CDS             complement(1995..3248)
                     /gene="hisS"
                     /locus_tag="PLA01_04925"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPD00678.1"
                     /translation="MLTQAPKGTKDILPSEIHKWHYIEKEIARICSDYGYKEIRIPVF
                     EHTELFQRGVGDTSDIVQKEMYTFSDKGGRSITLRPEGTAGVARSYIENGMASLPQPV
                     KLYYNITAYRYENVQKGRYREFHQFGVEAFGSYGHSIDVEIISMLRLLFDRLGIDQVK
                     LNINSIGCPVCRAEYNKKLMDYLRPNLDKMCPTCKTRFERNPLRIIDCKEQGCKKITA
                     GAPALIDHLCGECKTHFEGLKAGLDNLEIAYSVDKNIVRGLDYYTRTVFEFVSDNIGA
                     QGTVCGGGRYDGLIEECGGKPTPGIGFAIGIERLLMVMENQGIEFPQPGGIDIFIAAI
                     GDKAHKYAEKKVYELRKESLSAEKDLMGKSLKAQMKYADKLQAKYCIVLGDEEIESGK
                     AVLKNMKTGEEKAISLDTIIDRLKM"
     misc_binding    complement(3431..3671)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3797..4309)
                     /locus_tag="PLA01_04930"
     CDS             complement(3797..4309)
                     /locus_tag="PLA01_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00679.1"
                     /translation="MDTKEISEIPPEVPEIYFTVFDFSIDEVYYSVDEDIKKGASLKA
                     FTDANSYCWTEDLIEINENEECIVFFSKVKPMAEDVAKTYEIADYVFTNPVNLIFIKS
                     EDGYDVPKNLELFAKNGIEISEDELDINPDTMNQKEILRRQRRKNFLLVKDEKTLKEE
                     LKKIIEDVKG"
     gene            complement(4382..4570)
                     /locus_tag="PLA01_04935"
     CDS             complement(4382..4570)
                     /locus_tag="PLA01_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00680.1"
                     /translation="MFKKGLLIVAAIACTVSVLSAFDKQKVNSVNAENSTIVSNMQNL
                     KEVTPREEYIISQAKYAV"
     gene            complement(5047..5214)
                     /locus_tag="PLA01_04940"
     CDS             complement(5047..5214)
                     /locus_tag="PLA01_04940"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPD00681.1"
                     /translation="MSMGSAAAFMITGPSTKITNLGALKAILGLKQFIIYLLFVVAFS
                     VLTGTICNIVF"
BASE COUNT         1444 a         1268 c          773 g         1744 t
ORIGIN      
        1 tcgagccccc atcgcttttt ggtcatttca cttcgtgaaa tcccactaac aaaagagact
       61 ataagcaggt agacaatcaa tatatgcatt gtgcttatag tctctttata tttatatttg
      121 ttaattaata tttcttttaa gcatttaaaa agtatattat ttaaatgctt tcattattca
      181 aatcaaatat caaagatcca gttttatatg cagttcttta atctgctccg gatcgacatc
      241 gtccggtgca ttggacataa gacatgatgc attctgtacc tttggaaatg ctataacatc
      301 ccttatgctg cttcttccgg ccataagcat tacgagcctg tcaagcccaa aagccattcc
      361 cccgtgggga ggtgtaccat atttaaaggc ttcaagaagg aatccgaatc ttgcccacgc
      421 atcctcatct gaaaaaccta aaagtttaaa cattttagac tgcagttcct gactgtggat
      481 tctaatgctt ccgcctccga tttcgtttcc gttcagtacc atgtcatagg ctttagccct
      541 gaccttgccc ggatcggtat ccaacaattc gatatcttca tccatcggtg aagtaaaagg
      601 atggtgcttt gctacataac gtttttcctc ttcatcatat tcaagcaacg ggaactcagt
      661 aacccacaaa agcttaaact ccttactatc cgtgagtccc agttttcttg caatctccag
      721 tctcagctgt cctaaagaat cgaataccac ctcatccttg tctgcaacaa agcatatcag
      781 gtcaccgggc tgtgcttcaa ccctttgtaa aattgcattc atctcatcct cactgaagaa
      841 cttggttatg gaggacttaa gaccgttctc ttctacaact atccatgcca ttccctttgc
      901 cttgtatgtc ttaaccagtt ccaccagggc atctatttct ttacgcttga atttttcgcc
      961 gcatcccttt gcatttattg ccctgacgct tccgtcgcta ttaacggcat cggaaaacac
     1021 tttaaatccg cagttttcaa ccagatccga tacattgaca agctccagtc caaacctgat
     1081 atcgggcttg tcggatccaa acctttccat agcttcctta taaggcatcc ttaacaaagg
     1141 aatctcaata tcaacattta tagcttcctt gaacaatctc ttaataaatt cttcatttac
     1201 cgaaagcacg tcatcaacat ttacaaagga catctccaaa tctatctggg taaactccgg
     1261 ctgtctgtcc gccctaaggt cctcatccct gaagcacttg acaatttgga aatatctatc
     1321 gaaccccgaa accatcaaca actgcttaaa aagctgaggg gactggggca gtgcaaagaa
     1381 tttaccagga tgtactctgc tgggtataag gtaatccctt gcaccctcag gagtactctt
     1441 ggtaagcatc ggcgtctcta tttcgagaaa tcctttttca tcatagtaat ctctggcaat
     1501 ctttgaaacc ctgtgcctta acattaaatt cctctgcata tccggccttc tcaaatcaat
     1561 atacctgtac ttaagcctgg tggcctcatt tacatccgaa tcctcttcaa tatacatcgg
     1621 aggagtctca gcactgctga gtatcctgag ttcctttgca ataatttcaa tctctccggt
     1681 ggccatctcc ggatttattg cttcaggggc cctattcgaa accacaccgc ttactgccac
     1741 aacatattcg cccctgacag tactggcttt ttcgaaaatc tcaggatcac tctcatcgtt
     1801 gtacaccgtt tgtattatac cgcttctgtc tctgatgttt ataaatatta ctcctccaag
     1861 gtttcttgtt ctgtggcacc aacccatcac agtaacattt ttccctacat cattaatgcc
     1921 caacccggca cacatatgtg ttctctttaa tccacgaatt gattctccca tttatttaca
     1981 ctctcccttt tgaattacat ttttaaacgg tctattattg tgtcaaggct tattgccttc
     2041 tcttcaccgg ttttcatgtt tttaagcact gctttgccgg attcgatttc ttcatccccc
     2101 aaaacaatgc aatactttgc ctgaagcttg tcagcatact tcatctgagc cttaaggctt
     2161 tttcccatca agtccttctc ggcactaagc gattcttttc ttagctcata caccttcttt
     2221 tcggcatact tatgagcctt atcacctatg gctgcaataa atatatctat gcctcccggc
     2281 tgcggaaatt ctatcccctg gttttccata accattaaca gcctttcgat tcctattgca
     2341 aaaccgattc ccggtgtagg cttaccgccg cattcctcga taaggccatc ataccttcca
     2401 ccgccgcaaa cggtaccctg agcaccgata ttgtccgata caaactcaaa tacggttctg
     2461 gtataatagt caagtcctct tacaatgttt ttatcaacgc tatacgctat ttcaaggttg
     2521 tcaagcccag ccttcaagcc ctcaaagtgg gttttacact cgccgcaaag gtgatcaata
     2581 agagccggtg ctcccgcagt aatctttttg cagccttgtt ccttgcaatc aataatcctc
     2641 agcggattcc tttcaaacct tgttttgcat gtagggcaca ttttatccag attcggcctc
     2701 aggtagtcca tgagtttttt gttgtattct gccctacaga caggacatcc tatactgttt
     2761 atattaagct tgacctgatc tattcccaat ctgtcaaaca gcaatctcag catgcttata
     2821 atttcaacat caatggaatg tccataggag ccaaaagcct caaccccgaa ctggtgaaac
     2881 tccctatagc ggcctttttg cacattttca tacctgtagg cggtaatatt gtaatacagc
     2941 tttacaggct gcggcagcga tgccataccg ttctcaatat agcttcttgc aactcctgca
     3001 gtcccctcag gccgcaacgt tatactcctt ccacccttgt cggaaaatgt atacatttcc
     3061 ttttgcacaa tgtccgaagt atcaccaact ccccgttgaa acaactcggt atgttcaaat
     3121 acaggaatac ggatttcctt atagccataa tccgaacaaa tccgggctat ttccttttca
     3181 atataatgcc acttgtgaat ctcagacggt agtatatcct ttgttccttt aggtgcctgt
     3241 gttaacatca tatacctcca ttttttaaat attctcttat taatgctcct tgggcatcga
     3301 actcccattg ctttttggtc tcacttcgtg aaattcctca aaataaaaac catgaacatc
     3361 gaaccctttt gcatttcggt tctttcgcct agtgaaatcc cattaattaa agcctctcgt
     3421 ctgacaagac gtcagggacg agaggccttt attcccgcgg taccacccta ctaggcaaca
     3481 ctatcatgtg ttctgcccac tttacatcct tttaacgcaa gaacatgcgg gtcaatctag
     3541 tcgtataatt actttcaatc gaccggctca cgggggtctt tctccaacct gtttccttaa
     3601 aagtgcttcc agtcacggca ctttctccct taaaggcagt attggttact tctcccgttc
     3661 atagctttta aatacagtat ataaaattta atacaattat cgtcacatat tctaaagtta
     3721 aattattgct tttattttaa ttgttctggc gggaattgtc aatatcccat gaagttatta
     3781 tattatttgt atcctactac cccttaacat cttcaataat tttcttgagt tcttctttaa
     3841 gggttttttc atctttaaca agtaaaaaat tctttcgtct ttgtctcctt aatatctctt
     3901 tttgattcat tgtatcagga tttatatcca attcatcttc gcttatctca attccattct
     3961 ttgcaaatag ttctaaattc ttaggaacat cataaccgtc ttcgcttttg ataaatatta
     4021 gatttaccgg attagtaaaa acataatcag ctatctcata cgtttttgca acatcttcag
     4081 ccataggttt aacttttgaa aagaaaacta tgcactcttc attttcattt atttcaatca
     4141 aatcttccgt ccagcaatat gaattcgcat cagtaaaagc ttttaatgat gctcctttct
     4201 ttatatcttc gtctacacta taatatactt catctatact aaaatcaaaa acagtaaaat
     4261 atatttcggg aacctccggt ggaatttcag atatttcttt tgtatccata atgaattctt
     4321 tttttgattt tagagtaacc ttagctatta gatctgcata agatacggaa gttgcttcat
     4381 ctcatactgc atatttagct tgcgaaataa tatattcctc acgaggcgtt acttctttta
     4441 aattttgcat attacttact atcgtactat tttccgcatt aaccgaattt actttctgtt
     4501 tatcaaaagc agacaaaaca ctaacagtgc aagctattgc ggcaacaatc aataagcctt
     4561 ttttaaacat ctcataaacc ccccggattt tctattcaat agttacgagt tgtcttagtg
     4621 cagtacctat accaatttaa tataatctca aaactcttca gtcttgcagc acccatattt
     4681 taatgctatt ttataatact ttccaagata aatcatcata aaactgcgag ggatgtcaat
     4741 atcagacaat aaattatcct gtttagatat atttgattca attttcaatc cagaaagtta
     4801 ctaagactta tcgaataatt ataaaatttc tttttcaata ttagccaaaa atcctttacg
     4861 gcaagtatat attgaaacac tttgcagcaa gaggtaacca agtcactcta ttcttctttt
     4921 actttgcatt taatcccatc cattttataa tctgaaaaat cagtaatatt aataagtgcc
     4981 ttacaaagcc atcttaatat taccaacttt gaaattcctt acagccaata tgattgcatt
     5041 tttcatttaa aatacaatat tgcatattgt gcctgtaagg acagaaaatg caactacaaa
     5101 taataaatat ataatgaatt gtttcaagcc tagtatcgcc ttcaaggctc caagattcgt
     5161 tatttttgtc gatggtccgg ttatcataaa tgctgccgca gatcccatac tcatgccggc
     5221 atccagcca
//
LOCUS       DAOOJC010000132         5225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12663_length_5225_cov_3.979497, whole genome shotgun sequence.
ACCESSION   DAOOJC010000132 DAOOJC010000000
VERSION     DAOOJC010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12663_length_5225_cov_3.979497"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2313
                     /locus_tag="PLA01_04945"
     CDS             <1..2313
                     /locus_tag="PLA01_04945"
                     /inference="COORDINATES: protein motif:HMM:NF036757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00682.1"
                     /translation="DEAICKYFSTEEFSVMLPHIMSYVIPVALGDCYYGLGDYEEAEK
                     NYLAAADYKYINQRIEVPSLWIKLAQNVLAWGDMLYKEDMFQEALNVYRLVLEAPGSA
                     SVVNSESYLYKHNSLKITGSTVSKMLTEYEKTGNTGDINPQLAIVVLQVKERMIKLSG
                     GLDFRGIPLNLVPVWTFEYLQNVARYFAQQAIQAEREYIDFIETADNKKLTRQQLKQA
                     VDLAKAEVNLAKKQKDVAVSETKIYQEGLELAALRRQNMQVNKNAYSLMSWEKIRLDA
                     SNAWYSGPNTKIKGTDKKAYEFLFDNTLSMGTMSREYELGAMERQIAELRQAEEMAKA
                     QYSSAQARVSAAEQLERIAMLRETAAKENLIVFDNQTFTADVWTRMGNFMKNISDNYL
                     DMAINISRLMQKAYNFEFDAQQNSIKPNYSSKSINGLLASNALLLDIDSFTYDMINSV
                     KTKSIPIKQTLSLATRHPYLFETSFRTTGVMEFETYMEDFDMDYPGTYNRRIEAIEVI
                     VEGLIPSGGVKGFLTNCGISRYRTANFNTIKFRIQPKDTLVLSEYKVTGDSFVFSADP
                     AKLKLFEGAGICGSWKIEIPKFSNDLDYNYITDVKIVFYYKAFYNENLAQIVRNNLKT
                     LAGINKKSKILPLRYIFPDSYYYFLENGVFNFELDKSLFPYSESGFKISSLNLLAKNS
                     NGTGSKLTVLMKVPSGKVPLKIQTGSNVEITPTPIHPWVFLIGGDALGKYTIEILKED
                     NPSLVSDGKLQLESIEDLVLLLEYDYTPAL"
     gene            2480..>5225
                     /locus_tag="PLA01_04950"
     CDS             2480..>5225
                     /locus_tag="PLA01_04950"
                     /inference="COORDINATES: protein motif:HMM:NF023677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin TcdB middle/N-terminal domain-containing
                     protein"
                     /protein_id="HPD00683.1"
                     /translation="MSKTTGIAEQILSLPKGGGTVQGLGEKFKPDLHMGTGVYNIPLD
                     IPNGPNDIAPKLNLSYNTASGNGPFGMGYSLNLLAISRSTNKCIPNYTISDTLVLEGA
                     GKMLEISEGVFRVETDPYGWRIERYGEGFMLTDQNGIKYKLGTTEQSRLFKENNNRTE
                     TFRWLLEEIEDPLGYKAVFEYIRDGGNLYIKKISYSIYSIEFNYEKRNDSVINSRAGF
                     SIITNLRCSSIDLNICNSENPLIRKWILTYKESSPGKHSLLHKVELKGFDDKGNSESL
                     PVLTLDYTSFGSPQFTKFRGELSDVLPDSYDGGRRELVDFNGDGLPDLMEIRNGRAVV
                     WENKGNCLWGRPHSKDSFPLPVELEGQSVAFADMEGNGTADLLFMDRPLSGYYPLLPG
                     GGFGKPVFWRYSPHSKLSDTSTRLLDLNGDGITDLLCMGKDYYCLYYRDKEKGWIENP
                     VTLPKSLFPPVDINDDRVYFADMTGDGLQDIVKVDGSGVRFWPYLGNGIWGKEFKLKN
                     SVTIPNNFIPQRMFLADIDGDGCSDLIYVNSHRVLCWINTAGSQLSEPIEIFGTPLSD
                     ATNIRIADMKGTGTPGILWSYTAPGSRKTDYFYLDISGGNKPYLLNSIDNGMGLKTSI
                     SYSVSTLERQRDVFLGKPWNTFLPFPMQVVSSIVSADTITGVQTVNRYLYHDGHYEGS
                     DMEFTGFGSVEVIEEGDDSVPSMITRNHHHLGCDEKETLIPLPQNEKFQLKCLRGKLL
                     KTEVCGLDKDGKELLYYSTENKWDTKALNTADGREFTVPILVETRTMHFDKDAEVFRI
                     NSIENLEFDKYGNVLLQQQKAYNPLEPSGDKILNTRIFYAVSNEERFVNKPSRVIQTD
                     ESGKVFSLTLRYYDNMPEGLIGAKGLITNQEFLALDDETVQNIYSEDLPDFESLGYHR
                     K"
BASE COUNT         1641 a         1089 c         1123 g         1372 t
ORIGIN      
        1 gatgaggcaa tatgcaaata cttttctacg gaagagtttt cggttatgct cccccacatt
       61 atgtcctatg taataccggt tgcacttggg gattgctatt atggtttagg cgattatgaa
      121 gaggccgaaa agaattactt ggccgctgcc gactacaaat atataaacca aagaatcgaa
      181 gtaccttcat tatggataaa gctggctcaa aatgtattgg catggggaga tatgctttat
      241 aaagaagata tgttccaaga ggcactgaat gtttacaggc tggtactgga agcacccggt
      301 tctgcatctg tggtaaactc cgagtcatac ctgtataagc acaattcctt gaaaattacc
      361 ggcagtacag tatctaaaat gctcacggaa tacgaaaaga ccggcaacac cggcgatata
      421 aatcctcaat tggcaatcgt agttttgcag gtaaaagaaa gaatgataaa gcttagcggt
      481 gggttggatt tcagaggcat acccttaaat ctggttcctg tatggacttt cgagtatttg
      541 cagaatgtag ccagatactt tgctcaacag gcaatacagg ctgaaaggga atatatagat
      601 tttatcgaaa ccgccgataa taaaaagctt acaagacagc agttaaagca agccgttgac
      661 ctggcaaaag ccgaagtgaa tcttgcaaaa aagcaaaaag atgttgccgt cagtgaaaca
      721 aagatatatc aagaaggctt agaacttgcc gcattgagaa ggcagaatat gcaagtcaat
      781 aaaaatgcct actcactcat gtcctgggaa aagatacgtt tggatgcttc taatgcatgg
      841 tatagcggtc cgaataccaa aataaaaggt actgataaaa aagcgtacga atttcttttt
      901 gataataccc tcagtatggg caccatgtcc cgcgaatatg agttgggtgc aatggaaagg
      961 cagatagcag aattaagaca ggctgaagag atggcaaaag cacagtactc aagtgcacag
     1021 gccagggtgt cggcagcgga acagctggaa agaatagcca tgcttcgtga aacagctgca
     1081 aaagaaaact tgatagtttt tgacaatcaa accttcaccg ccgatgtatg gactagaatg
     1141 ggaaacttta tgaagaacat atcggacaat tacctcgata tggctatcaa catttcccgt
     1201 ttgatgcaga aggcctataa cttcgaattt gatgcccagc aaaactctat caaacccaac
     1261 tacagttcaa aatccataaa cggcttactt gcatcaaatg cactcttact ggatatagac
     1321 agcttcacct atgatatgat aaattcggta aaaacaaaga gtattcctat aaaacaaacc
     1381 ctgtcattgg ccacccggca tccctatctc tttgaaacct ccttccgcac taccggagtg
     1441 atggaattcg aaacctatat ggaagatttt gatatggatt atccgggtac ttacaataga
     1501 agaatagaag ccattgaggt catagttgaa ggattgattc catcgggcgg tgtaaaaggc
     1561 tttcttacca attgcgggat aagccgctac agaacagcaa atttcaacac tataaaattc
     1621 cgcatccaac cgaaagatac cttagttctg tccgaatata aagtaaccgg agattctttt
     1681 gtattcagtg ccgatccggc gaaattgaag ctctttgaag gagccggaat atgcgggagc
     1741 tggaaaatcg aaataccgaa attcagtaat gacctagatt ataattatat taccgatgta
     1801 aaaatagttt tttactataa ggccttttac aatgaaaatc ttgcacaaat tgtcaggaat
     1861 aatcttaaga cgctcgcggg gatcaataaa aaatcaaaaa tactgcctct tcgttatatt
     1921 ttccccgatt cctattatta tttcctggaa aatggagttt tcaatttcga actggataaa
     1981 agtctgtttc cgtacagtga atccggcttt aaaataagca gcctgaacct tttggcgaaa
     2041 aattcaaatg gtaccggttc caaactgact gtgctaatga aggtgccatc cggaaaagtt
     2101 ccgctaaaaa tccagacagg ttcaaacgtt gaaattacac ctactccaat acatccatgg
     2161 gtttttctta taggcggaga tgctttggga aaatatacca tcgagatcct caaggaagac
     2221 aatccgtcct tggtatccga cggcaaactt cagctggaaa gcattgaaga ccttgtgtta
     2281 cttttggaat acgactacac cccggcgtta taacgaggct ataaatgtcc cccacttttt
     2341 ataagtggtt gataccaatt agcttttctg gcggtgaata atcttttgcc tcgttgaaac
     2401 accgctaatg taagaaaatt tttctacaat cgaaaaattt tctttgaacc gaggtgttat
     2461 aattctagga ggtgtttaaa tgagtaaaac aacaggaatt gcagaacaaa ttctgtcact
     2521 gccaaaagga ggaggaacag tacagggtct tggtgagaaa tttaagccgg atcttcatat
     2581 gggtacaggg gtatataata taccccttga tatccccaat ggacctaacg atattgcccc
     2641 taaactgaac ctctcgtaca acacagcttc gggcaatggt cctttcggta tggggtattc
     2701 attaaatctt cttgccataa gccgctctac caataaatgc atccccaact acaccataag
     2761 tgataccctg gtcctagagg gtgccggaaa gatgttggaa atttcagagg gagtttttcg
     2821 cgttgaaacc gatccctatg gctggcgtat tgaacgctac ggcgaaggct ttatgctcac
     2881 ggaccaaaac ggtattaagt acaaacttgg aacaactgaa cagtccaggc tttttaagga
     2941 gaataacaac cgtaccgaaa ccttcaggtg gcttttggaa gaaatagagg atcccctcgg
     3001 atacaaagcc gttttcgaat atataaggga tggcggaaac ctttatatca aaaaaatctc
     3061 ctacagtatt tatagtattg aatttaatta tgagaagagg aacgattcgg tcataaattc
     3121 aagggccggt ttttcaataa taaccaatct cagatgcagc agtatcgacc ttaatatctg
     3181 taattcggaa aatcctctta taagaaaatg gattcttacc tataaggagt cttcccccgg
     3241 aaagcattcc ctcctccaca aagtagagtt aaaaggcttt gatgataagg gaaacagtga
     3301 aagtctgccg gttttgaccc tggactatac ctcctttggc tctcctcaat ttactaaatt
     3361 ccgcggcgag ctttcggatg ttcttccgga ttcatacgac ggcggaagaa gagaacttgt
     3421 tgacttcaac ggagacggtc ttcccgattt aatggaaatc aggaacggaa gagctgtggt
     3481 ttgggaaaat aaaggtaatt gcctctgggg tcggcctcat tccaaagaca gcttccccct
     3541 gcccgtagaa cttgaaggtc aaagcgtagc ctttgcagac atggaaggca acggtactgc
     3601 ggacttgcta tttatggatc gacccctctc gggttattat ccccttttac cgggaggagg
     3661 attcggaaaa ccggtattct ggagatactc cccccactca aaactcagcg ataccagtac
     3721 cagattattg gatttaaacg gtgacggtat aaccgatctt ttgtgcatgg gaaaagacta
     3781 ttattgcctc tattacaggg ataaagaaaa gggctggata gaaaaccccg tcacccttcc
     3841 gaaaagcctt ttccctccgg tggatataaa tgatgaccgt gtgtattttg ccgacatgac
     3901 gggggacggt ttgcaggata ttgtaaaagt agacggctcc ggcgtgagat tttggccata
     3961 ccttggaaac ggaatctggg gcaaagaatt caagctcaaa aactccgtta caattccgaa
     4021 taatttcatt ccccaaagaa tgtttcttgc cgacattgac ggtgacggtt gctccgactt
     4081 gatttacgtc aactcccacc gggttctgtg ttggattaac actgcaggct ctcaactgag
     4141 tgaacccatc gagatattcg gaactcccct ttcggatgct acaaatatac gtattgcaga
     4201 catgaagggt accggcaccc caggcatact gtggtcttat actgctcccg gaagcaggaa
     4261 aaccgattat ttctaccttg atatatcggg cggaaataag ccttatcttc taaacagtat
     4321 cgacaacgga atggggctca aaacatctat atcgtacagc gtctccacat tggaacgtca
     4381 aagagatgtt tttttgggca agccttggaa tacatttctg ccctttccca tgcaggttgt
     4441 ctcttctatc gtatctgccg ataccatcac cggggttcaa acagtaaacc gctatttgta
     4501 tcatgacggg cattacgaag gaagcgacat ggaatttaca ggtttcgggt cggtagaagt
     4561 aattgaggaa ggagacgatt ctgtcccgtc aatgattact agaaaccatc accatctggg
     4621 ttgtgatgaa aaggaaacct taatacctct tccccaaaat gaaaaattcc aattaaaatg
     4681 cttgcgggga aaactattaa agacagaggt ttgtggcctt gataaagacg gaaaagagct
     4741 cctttattac tcaaccgaaa acaagtggga tactaaagcc ttaaatactg ccgacggcag
     4801 ggagttcaca gttcccattc ttgttgagac ccgtacaatg cactttgaca aagatgccga
     4861 agttttcaga ataaattcta tcgaaaacct cgagtttgat aaatacggaa atgtactctt
     4921 acaacaacaa aaagcctata acccccttga accttcaggg gataaaatac tcaatacaag
     4981 gattttttac gctgtaagca atgaagaaag gtttgtcaac aaaccatcca gagtaattca
     5041 gaccgacgaa agcggaaaag ttttttcact gactctgcgg tattacgaca atatgcccga
     5101 aggacttatc ggtgccaagg gtcttattac caatcaagag tttctggcat tagatgacga
     5161 gaccgttcaa aatatttatt ccgaggacct tccggatttt gagtccctgg gctaccaccg
     5221 caaaa
//
LOCUS       DAOOJC010000133         5138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12911_length_5138_cov_4.066103, whole genome shotgun sequence.
ACCESSION   DAOOJC010000133 DAOOJC010000000
VERSION     DAOOJC010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12911_length_5138_cov_4.066103"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..2857)
                     /locus_tag="PLA01_04955"
     CDS             complement(101..2857)
                     /locus_tag="PLA01_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPD00684.1"
                     /translation="MKKALCKKVCLITVSLCMILTTICQNVAFLSAEAPVAQKVVETP
                     EQVDKQDAEITVEIPGIFDVSGITVDTGKVTNYSINGDEVTVSVTDGEPKREGPDTRH
                     IAVTESYDHDNFPAVFEYSRDGYTGSLPKVDSGYSADKKKYWAQYEGEITKDNCNFYS
                     YVITIDYILNVAPDLSLDLEGYTINSKLDVIGDIVDENIGDSLQIYYSIDVNNGVGTP
                     FLDTITSNGLKQDVSGHIDVPSNLSEGKHNIFFWAVDKKGLKSVPFKIAFTVDRTPPE
                     APILIPDKTEPTNTSVTVSVYYPDDAYIKEVKIGDGDWVPIGSITDNDELIVEENTTI
                     EARATDEAGNVSPEGKLVIDNIDKIPPEAPVLIPDKTEPTNTSVTVSVYYPDDADKKE
                     IKIGDGDWLPIDEVTDDGKITVEENTTIEARATDEAGNESPITTLVIDNIDKIPPGAP
                     TIKTSATETTGSSITATIEPGEDEGSGINRTEYCLKGATQKDWSTYEPDSVITITSKG
                     ETEIWARSIDNAGNVSDIAKKTVAIKSGSGGSGGSGGSGGNNNPGNQDNDKDKDNNKD
                     KDKEVTEPGNNVPGAGPVDLSIFVSADKSKYAEDDIITLELNYKNKSGISVSNVVVKA
                     EIPPYTTPVETANGTVSENYIEWKISSLNANSSAKIQYTLKVGQLDKAEVNSSVIASI
                     TSDGTLINKDDDESKTLFLLYSDRYKDNFHKKYIAGYEDNTFRPFNNITRAEVATIMA
                     NVLDIKGEITSSRSYTDLSESHWAYKNIMAVTEKGLFTGYLDGSFHPDSYITRAEFAT
                     VLANYLQLKNVEHDRLNFSDISNHWAKNFIEEIYRIRLIEGYIENGERLFKPDSNITR
                     SEAVTIINKMLFRGPLFGAEVPFNDVEEDYWAFGHIAESSIDHFYTRNEDESETIANK
                     EDEE"
     gene            complement(3192..3267)
                     /locus_tag="PLA01_04960"
     tRNA            complement(3192..3267)
                     /locus_tag="PLA01_04960"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3232..3234),aa:Lys,seq:ttt)
     gene            complement(3273..3349)
                     /locus_tag="PLA01_04965"
     tRNA            complement(3273..3349)
                     /locus_tag="PLA01_04965"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3314..3316),aa:Gln,seq:ttg)
     gene            complement(3358..3434)
                     /locus_tag="PLA01_04970"
     tRNA            complement(3358..3434)
                     /locus_tag="PLA01_04970"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3398..3400),aa:His,seq:gtg)
     gene            complement(3576..>5138)
                     /locus_tag="PLA01_04975"
     CDS             complement(3576..>5138)
                     /locus_tag="PLA01_04975"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPD00685.1"
                     /translation="FYEQRWFASGKNAVNNTVFLCGQEVPFGNDLLFEDAGENICFGV
                     ELCEDLWVPVPPSAYQTMAGALLIFNLSASNEVIGKYEYRKELVRQQSAKCLAGYVYT
                     SSGINESTTDVVFGGHALICEDGTLLSESKRFESDEQLIFSDIDSQRLMNDRRKNTSF
                     MELWNESSKSFRKVRVELPKYKTEGLMRYVAPHPFVPSNEKSRDERCSEIFSIQTAAL
                     QKRIKHTGLKHAVIGISGGLDSTLALLVTAKAIDNLGIPRKNIVAVTMPGFGTTDVTY
                     SNAMELMKSMKVDIREIDIKAACIQHFKDIGHDPAVHDVTYENVQARERTQILMDIAN
                     KEGGLVIGTGDLSELALGWCTYNGDHMSMYSVNCSIPKTLVRYLVRWIADNVADERSK
                     NVLIKILETPISPELLPPDEKGNIQQKTEDIIGPYELHDFFMYHMLRYGATPEKIAFL
                     AEKAFEGKYSKETIKKWLTVFIKRFFSQQFKRSCLPDGPKVGTISLSPRGDWRMPSDA
                     VASSWLSELEDL"
BASE COUNT         1412 a         1196 c          861 g         1669 t
ORIGIN      
        1 acccgtggct agactttcac tagctagatt agtgccatgc ctggcacaca attaaaggag
       61 agcttcaaaa atccgaagcc ctcctttgat ctcgtcaagc ttattcttcg tcttccttat
      121 ttgctatagt ctcactctca tcttcatttc ttgtgtagaa atggtctatt gagctctccg
      181 ctatatgtcc gaatgcccag tagtcttctt caacatcatt gaagggtact tctgccccaa
      241 acagcggtcc tctgaacaac attttgttta ttattgttac tgcttcgcta cgggttatat
      301 tcgaatcagg cttaaacaat ctttctccgt tttctatata accctctata agtcttattc
      361 tgtatatttc ttcaataaag tttttagccc agtgattact aatgtcagaa aagtttagcc
      421 tatcatgttc aacattctta agttgcagat aatttgcaag aactgtcgca aattctgccc
      481 tggtaatata gctgtcgggg tggaacgaac cgtcaagata ccctgtaaac aaacctttct
      541 ccgtaacagc cattatgttt ttgtatgccc aatgactctc ggataaatcc gtatatgacc
      601 tgctgctggt aatctctccc ttaatatcca acacattagc cataattgtt gcaacttcag
      661 ctcttgtgat gttattgaaa ggacggaacg tattgtcttc atagccggca atatacttct
      721 tatggaaatt atccttatac ctgtctgagt acagcaggaa taaagttttt gattcatcat
      781 catccttgtt tatcagcgtt ccatccgaag taatagaagc aataacagaa gaatttactt
      841 ctgccttatc caattgacca accttgaggg tgtattgtat tttagcagaa gagtttgcat
      901 taaggctact tattttccat tcaatgtaat tctcacttac cgtaccgttt gcagtctcta
      961 caggcgttgt ataaggcggt atttctgctt taacaacaac attgcttaca gaaatacctg
     1021 acttattttt atagtttagt tccaaggtta ttatatcgtc ctcggcatac tttgatttat
     1081 cggcactaac gaaaatcgac aaatccacag gacccgcacc gggtacgtta ttgccaggtt
     1141 ctgtaacttc tttgtcctta tctttgttgt tatctttatc cttatcatta tcctgattac
     1201 ccgggttatt attgccgcca gaacctccag agcctccaga accaccggaa ccggacttta
     1261 ttgcaacagt tttctttgca atatccgaca cgttcccagc attatcaatt gaccttgccc
     1321 aaatctctgt ttcacctttt gaagtaatag taataacgct gtcaggctca tatgtactcc
     1381 agtccttctg agttgcaccc tttagacaat actctgttct attaattcct gatccttcat
     1441 cctctcccgg ttcaattgtt gcagttattg aagatccggt agtctcggta gcacttgttt
     1501 taattgtcgg tgctcccggg ggaattttgt caatattgtc aattaccagt gttgttatcg
     1561 gtgattcatt gcctgcctca tctgttgctc tggcttcaat ggttgtattc tcctccaccg
     1621 ttattttgcc gtcatcggtt acctcatcta tcggaagcca atctccatca cctattttta
     1681 tttctttttt atctgcatcg tcaggataat atactgacac agttacactc gtatttgtgg
     1741 gttctgtttt atccggaatg agtaccggtg cttccggtgg aattttgtca atattgtcaa
     1801 ttaccaattt tccttccgga gatacattcc ctgcttcatc tgttgctctg gcttcaatgg
     1861 tcgtattctc ctccactatt aactcgtcat tatcggttat actgcctatc ggcacccaat
     1921 caccatcacc aatttttact tctttaatat atgcatcgtc aggataatac actgacacag
     1981 ttacacttgt atttgtgggt tctgttttat caggaataag tatcggtgct tccggtggcg
     2041 ttctgtcaac cgtaaaagcg attttaaaag gaacagactt taaacctttt ttatcaactg
     2101 cccagaagaa gatgttatgc ttaccttcac taagattcga aggaacatca atatgaccac
     2161 ttacatcctg ttttaatccg tttgaagtaa tagtgtctaa gaaaggagtt cctactccat
     2221 tattaacatc gattgaataa tagatctgta aagaatcacc aatgttttcg tcaacaatat
     2281 ctcctataac atcaagttta ctgtttattg tatagccctc taaatccagg gaaaggtcag
     2341 gagcaacatt taaaatatag tctattgtaa tcacataact atagaaatta caattatctt
     2401 ttgttatttc tccttcgtac tgtgcccaat acttcttttt atctgcactg taaccgctgt
     2461 cgactttcgg caaactgccg gtatacccat ctcgtgaata ttcaaataca gccgggaaat
     2521 tatcatgatc gtatgattct gttacggcta tatgcctcgt atcaggtcct tctctttttg
     2581 gttcaccatc agtaacactg acagttacct catcaccatt tatgctgtaa ttggtgactt
     2641 tccccgtgtc cacagtaata cctgaaacat cgaaaatacc ggggatttcc acagtaattt
     2701 cggcatcttg cttatctacc tgttcaggag tctctaccac tttctgtgca acgggagcct
     2761 ctgctgataa aaaagccaca ttttgacata tggttgtcaa aatcatacaa agacttactg
     2821 taattaaaca aactttctta cataatgcct tcttcatttt ctactctccc ttactattta
     2881 ttttttattg ttgaaaattg aaaatacggt ttcctgtatt taccccataa tataatatac
     2941 atttaagata tttttttcaa ctatatattg tgtaatatat gttaatattt aggatattta
     3001 tggaaaaaat atataaataa ttatgatatt aggcatatca aactaaaaac catttttcaa
     3061 tgcaattata aataggaaat taaacccgta tcttaggttc catctacatt tttttaattt
     3121 acacccatca acagtaagta catttccaga caataaaaaa cccttacaac cgttaacagt
     3181 cgtaaaggtt ttggtgagcc atcggggagt cgaaccccgg acaccatgat taaaagtcat
     3241 gtgctctgcc aactgagcta atggctcgaa tatggcaggg atggcaggac tcgaacctac
     3301 gaaatgccag agtcaaagtc tggtgcctta ccgacttggc tacatcccta taatttgtgg
     3361 ggtgaatagt gggagtcgaa cccacgacct ccagagccac aatctggcgc tctaaccaac
     3421 tgagctatat ccaccgaaaa tgtgccccaa aaaaggcaca aggatatttt aagacagata
     3481 ataataaatg tcaagcagtt tttttatttt tcctttacgt aattaatagc aataatacat
     3541 ctaaacaaat atgtctccaa agctttacaa tacacttata aatcttccag ttccgaaagc
     3601 catgaacttg ccacagcatc actgggcata cgccaatctc ccctcggcga aaggctgatc
     3661 gttcccacct tgggtccgtc cggcaaacat gaccttttaa actgttggct gaaaaacctt
     3721 tttataaata ctgtcagcca tttctttatc gtttccttgg aatatttgcc ctcaaaagct
     3781 ttctctgcaa gaaacgcaat cttttcaggt gttgccccat accttaacat gtgatacata
     3841 aaaaagtcat ggagttcgta agggcctatt atatcctcgg tcttctgctg gatatttccc
     3901 ttctcgtcag gaggaagaag ctccggagaa atgggagttt ccaatatttt aattaagaca
     3961 ttcttcgacc tctcatccgc cacattgtct gcaatccacc ttaccaaata ccttactagg
     4021 gtttttggta tactgcaatt caccgaatac atggacatat gatcaccgtt ataggtgcac
     4081 caccccaacg ccaattccga aaggtcaccg gtgcctatca caagtccgcc ttccttgttg
     4141 gcaatatcca tcaaaatctg agtgcgttcc cgggcctgaa cattttcata agttacgtca
     4201 tgtaccgccg ggtcatgtcc tatatcctta aaatgctgaa tacaggcagc cttaatatca
     4261 atttcccgta tatccacctt catagatttc attaactcca tggcattgga gtaagttacg
     4321 tcagttgtcc cgaatcccgg catagtaacc gcaacaatgt tctttctggg aataccgaga
     4381 ttatcaatgg cttttgcggt taccaaaagg gccagtgtgg aatcaaggcc tcctgaaata
     4441 ccaataacag catgcttaag tccggtatgc tttatcctct tttgcaatgc agcagtttga
     4501 atggaaaata tctcactaca tctttcatca cggctttttt cattggaagg tacaaaggga
     4561 tggggggcta catatctcat taatccttca gttttgtatt tcggaagctc taccctcact
     4621 tttctgaagc tcttggaact ttcattccac agctccataa agctggtatt ttttcttctg
     4681 tcattcatca acctctggct gtcaatatcc gaaaatataa gctgctcatc gctttcaaat
     4741 ctttttgact cgctcaaaag agttccgtcc tcgcagatca atgcatggcc tccgaaaaca
     4801 acatccgtag tcgattcatt tataccggaa gaagtgtaga cataacccgc caggcatttt
     4861 gccgactgct gccggaccag ttctttacgg tactcatact ttcctataac ttcattgctg
     4921 gcagaaagat taaaaattaa caatgccccg gccatcgtct gataagcact gggtggaacc
     4981 ggaacccata ggtcctcaca caattcaaca ccaaagcata tgttttcccc ggcatcttca
     5041 aaaagcaaat cattcccaaa gggaacttcc tgaccgcaca ggaacaccgt attgtttaca
     5101 gcatttttcc cggacgcaaa ccatctctgt tcataaaa
//
LOCUS       DAOOJC010000134         5127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12942_length_5127_cov_3.585568, whole genome shotgun sequence.
ACCESSION   DAOOJC010000134 DAOOJC010000000
VERSION     DAOOJC010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12942_length_5127_cov_3.585568"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="PLA01_04980"
     CDS             <1..1177
                     /locus_tag="PLA01_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPD00686.1"
                     /translation="LVAKITPENATNKEIVWTTSDDDIAKVSSTGMVTAVSAGTVTIT
                     AASKEDQKIKAVCTITVKETTVSDISLSQRTVTLAVGQSITLKVDFYPSSANNKGLDW
                     KTNNSSVAQVSSGGVVTAKAVGTAIVTVTSKANPSCKDTCTVYVEEALPIPTEPVYDP
                     GVIPGAGGGDVPIVSIDETPSPSAKPTPTPSGTSSPAVTQTPTQGNDKPGSLDSFTDI
                     KGHWAATFFEDLLQKGIVSGYPDKTLRPNAPITRAEAAVMVMKAAGFDISGNTALTCT
                     DKDTVPDWAKAYVATAMYKGVVKGYEDGSFRPSNRLTRQETVVLVLRAFGIEEAQDKS
                     LSFADSNTIPAWSAGYIKKAVELGIIKGYSDNTFGPTREITRAEVVTIISKCMQLKK"
     gene            1452..2210
                     /gene="sigI"
                     /locus_tag="PLA01_04985"
     CDS             1452..2210
                     /gene="sigI"
                     /locus_tag="PLA01_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-I factor"
                     /protein_id="HPD00687.1"
                     /translation="MDARKNKGTRKEKHMDESVYDAFIFTLKQIKEGNQQLRDEFIAE
                     YKPFILKVTSNALGKYIDTRNNDEFGIAMSAFNEAIDRFDPAKGYNFFLFAEQVIRRR
                     LIDYFRRNKDNKEYPFSYFEDDNDYYDESWISDSFTGFEDVEIQEEIDNFKKKLSEFG
                     ITFVDLVLNVPKHKDSRRLCIKIAQMLAEDDQLYRTLMRNKNIPRNELKKKAKVHGRT
                     IGNNRKYIIALCLILKSNLSLSKRYLEYTMEGGR"
     gene            2212..3705
                     /locus_tag="PLA01_04990"
     CDS             2212..3705
                     /locus_tag="PLA01_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor domain-containing protein"
                     /protein_id="HPD00688.1"
                     /translation="MNRTGIIYDIQNNRAVVLTSESEFVMVKKRRDMSIGQQVRFENR
                     DVFSPNRRLLLNAVALAGVAAVLIVTFLCYRFFLPGNNWNVYGYIGVDINPSVEFEIK
                     KNHRVLKMRPLNEEGKQLIRELDINDKKIEDAVSELIGKSIDMGFIESVDERIVLISG
                     ALKDEKSKESSGNDKEETRLYNLLNNIKSEIDNDRVEGHIILLGSQERKNALEKNIPM
                     GKYGLYLEAEELKAGITFEDIYDMSVAEMIEKLNKIEPQESADVSNSENSPIPTPKHD
                     IKTPKSEISTPPLSQKNDSVQEPTSIKTLTPNPTATHIDTRRPGTSELPTNTPVGTLE
                     PTQKSMPTPMKVPAKGSLRIQYYSKADKEIVQGIDYSFRMFNTTSSVIDMRNVKVRYY
                     FKEGVPINSMNSAIYFYSLGKEEDVHCKFYNLSGSDKANNYLEITFSSGSLSPDEILY
                     ITAMFYKNDWGEFDQSDDYSYNPMDTYVDWENMTAYINDVLVWGQEP"
     gene            3818..4312
                     /locus_tag="PLA01_04995"
     CDS             3818..4312
                     /locus_tag="PLA01_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HPD00689.1"
                     /translation="MSIFAQWNEIVEKERTKEENDKLWEEYLMKEKNMYEYILEHHDE
                     AVSGKLTELAQKFNVDAVSFVGFIDGINTSLKESIVLEDLKEDSDIKFDVDFEKLYYN
                     MLDAKAHWLYNLPQWDGVLTQEKRNEITKDYNKSHIAVSNKVGRNDPCTCGSGKKYKK
                     CCGA"
     gene            4297..4740
                     /gene="lspA"
                     /locus_tag="PLA01_05000"
     CDS             4297..4740
                     /gene="lspA"
                     /locus_tag="PLA01_05000"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPD00690.1"
                     /translation="MLWCLKPVFCYLFIAVLVVIDQVSKKYAASKLKGVIFPIRAGIF
                     RFIIVKNPGAALGFLRKKQSFLKIITLIMVIFLLTYTTLSLIYKEPVKDILSLALISG
                     GALGNFVDRIRHKYVIDFISLNIKRFPVFNMADVFIATGFVLYIV"
     gene            complement(4758..>5127)
                     /locus_tag="PLA01_05005"
     CDS             complement(4758..>5127)
                     /locus_tag="PLA01_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HPD00691.1"
                     /translation="TFILLGAIGIPVFSKGGAGLAYLSGPTGGFIIGFLIGAITISLL
                     KGKDCNILRTGVACAMGGIIVVYAIGIPWLCYERTGNFLSLPLLLANLAFIPGDILKV
                     CIASIVSVKVRKHLKYLLTE"
BASE COUNT         1744 a          833 c         1149 g         1401 t
ORIGIN      
        1 gcttgtggca aaaataacac cggagaatgc aacaaataaa gagatagtct ggacaacgag
       61 tgacgatgat attgccaagg tatcatcgac cggtatggtt acagccgttt cagcaggaac
      121 tgttacaata actgcagcaa gcaaggagga tcaaaagatc aaggctgtat gtaccataac
      181 tgtaaaagaa acaacggtaa gtgatataag tcttagccaa aggactgtta cccttgcagt
      241 tggtcaatca attactttga aggttgattt ttatccttct agtgccaata ataagggatt
      301 ggattggaaa acaaacaata gttctgtggc acaggttagc agcggaggag ttgttacagc
      361 aaaggctgta ggaactgcaa tagtaacggt aaccagtaag gcaaacccgt catgcaagga
      421 tacatgtacc gtttatgttg aagaggcact tcctataccc actgagcctg tttacgatcc
      481 cggtgttatt ccaggtgcag gaggcggaga tgttccgatc gtatctattg atgaaacacc
      541 gagtccaagt gctaagccta cgcctacacc gagcggtact tcatcacctg cagtgaccca
      601 aacaccaacg cagggtaacg ataagccggg cagccttgat tcctttacag atataaaggg
      661 acattgggca gcaacatttt ttgaagattt gttgcaaaaa ggaatagtaa gcggatatcc
      721 ggataaaact ctcagaccta atgcaccgat aaccagagca gaagctgctg taatggttat
      781 gaaggcagcg ggatttgata tatccggcaa cactgcactt acatgcaccg acaaagatac
      841 ggttccggat tgggcaaagg catatgtcgc aactgcgatg tataaaggag tggttaaagg
      901 atatgaagat ggttccttta gaccttccaa caggttgaca cgacaggaga cagtggttct
      961 ggtactgaga gcttttggaa tagaggaagc tcaggataag agcctatcct ttgccgattc
     1021 aaatacgata ccggcttggt ctgcaggtta tataaagaaa gcggttgagt tgggaataat
     1081 taagggctat agtgataata cctttggccc gacaagagag ataacccgtg cagaggttgt
     1141 taccatcatt tcaaagtgta tgcagcttaa gaagtaataa atctctgaaa agttaatatg
     1201 tattgtttaa tgaaaggagt ccggggatgt caacccgggc tcctttacct ttcttctcta
     1261 taatgatatg tacccttaat gaaggaaaag ggttggttcc tgtggaaaag aatatatcgg
     1321 tgaagtatga agggttattt ttcgatataa aaagtattaa agtttttttg aaattggaat
     1381 atacgatgat atcatgcaca taaaaaaatc aaacctgttc gaagttatgt accgaaggga
     1441 gaatgatcta gttggatgcc cggaaaaaca aaggtaccag aaaagaaaaa cacatggatg
     1501 aatcagtcta tgatgctttt atttttaccc tcaaacagat aaaggaaggg aatcaacagc
     1561 ttagagatga gtttatagcc gaatataagc cgtttatact aaaggtaaca tcaaatgcac
     1621 tgggcaagta tattgatact cgcaataatg acgagtttgg cattgccatg tctgcattta
     1681 atgaagctat tgaccggttt gaccctgcta aaggctataa tttttttctt tttgccgaac
     1741 aggttataag aaggcggttg attgactatt ttaggagaaa caaggataat aaggagtatc
     1801 cgttttcata ttttgaagac gataatgatt attacgatga aagctggata tccgattcat
     1861 ttaccgggtt tgaagacgtt gagatacagg aggaaattga taattttaaa aagaagctga
     1921 gtgaattcgg aataaccttc gttgatttgg tattaaacgt accaaaacac aaggattcaa
     1981 gaagactatg cattaagata gcccaaatgc tggcagaaga tgatcagcta tacaggacat
     2041 tgatgagaaa taagaacata ccgagaaatg agttgaagaa aaaagccaag gtacatggaa
     2101 gaaccatagg aaataataga aagtatatta tagctctttg tttgatttta aagagtaacc
     2161 tcagcctttc aaagagatat ttggaatata ctatggaggg gggaagataa catgaataga
     2221 acgggaatta tatatgatat tcagaataac agagctgtgg ttttaacatc tgaaagcgaa
     2281 tttgttatgg ttaaaaaacg ccgggacatg agcatcggac agcaggtgag gtttgaaaac
     2341 agggatgttt ttagccctaa cagaagatta cttttaaatg ctgtagctct tgccggtgtt
     2401 gcagcagttt tgattgtgac atttctatgc taccggtttt tccttccggg aaataattgg
     2461 aatgtatatg gttatatcgg tgttgacata aatcccagtg tggagtttga aatcaagaag
     2521 aaccatcggg ttttgaaaat gaggcctctc aatgaagaag ggaaacaact aatccgggaa
     2581 ttggatatca acgacaaaaa gattgaggat gccgtttccg aattaatagg aaaatctatt
     2641 gacatgggct ttattgaatc ggttgatgag agaatagtac ttatttccgg ggcactaaag
     2701 gatgaaaaga gtaaagaaag ctccggtaat gataaagagg aaaccagact gtataattta
     2761 ctgaacaata taaaaagcga gatagataat gacagagtag aagggcacat aattttgcta
     2821 ggctcacaag aaagaaagaa tgctttggaa aaaaacattc ccatggggaa atacggtttg
     2881 tacttagaag ctgaagaatt gaaggcaggc ataacctttg aagatatcta tgatatgagt
     2941 gtagctgaaa tgattgaaaa attgaataaa atcgaaccac aagagtcagc tgatgtttca
     3001 aattctgaaa attcaccgat accaacgccg aaacatgata taaaaacgcc gaaatctgag
     3061 atatcaactc caccgttatc gcaaaaaaat gattcagttc aggaaccgac atcaattaaa
     3121 acattgacac ctaatccaac tgcaacgcat attgacacaa gaaggcctgg aacttctgaa
     3181 ctgccgacga acacaccggt agggacattg gagccgaccc aaaaatcaat gccgactcca
     3241 atgaaagtac cagcaaaagg aagcttgaga atacagtatt atagcaaggc tgataaagaa
     3301 attgtccagg gaattgacta tagctttaga atgtttaaca ctacaagcag cgtaattgat
     3361 atgagaaatg taaaagtaag gtactatttt aaggagggtg tccctataaa cagcatgaac
     3421 agtgcaatat atttttacag cttgggaaaa gaagaggatg ttcactgcaa gttttataat
     3481 ttgtcaggtt ccgataaagc gaataattat ctggaaatta cttttagttc gggaagcctt
     3541 tcccctgatg agatacttta tataacggca atgttttata aaaatgattg gggtgaattt
     3601 gatcagagtg atgactattc gtataacccg atggatactt atgtggattg ggaaaacatg
     3661 accgcctata taaatgatgt actggtatgg ggacaggagc cttagtgctg cagttttttc
     3721 cttcaccttt attatttttt gcttggggtt ataattatta tgggaattgt gctataatat
     3781 aaaaataaaa caggataaaa ggagagattc agtacttatg agtatttttg cacaatggaa
     3841 cgagattgtt gaaaaggaaa gaacaaagga agaaaatgat aagctttggg aagaatattt
     3901 gatgaaagag aaaaatatgt atgagtacat actggaacat catgatgagg ctgtaagcgg
     3961 aaaacttact gagcttgcac aaaagtttaa tgtggatgcg gtttcatttg ttggttttat
     4021 agacggcatc aacaccagtc tcaaggaaag tattgtgctt gaagacttga aagaggattc
     4081 ggatataaag tttgatgtgg attttgagaa actttactat aacatgctgg atgcaaaagc
     4141 acactggctg tataaccttc ctcaatggga tggtgtactg actcaggaga aaaggaatga
     4201 aatcacaaag gattataaca aatcccatat agcagtcagc aacaaggtgg gaagaaacga
     4261 cccctgcaca tgtggcagcg gcaaaaagta taaaaaatgc tgtggtgctt aaaaccggtt
     4321 ttttgttatt tatttattgc tgtccttgtt gttattgacc aggtaagcaa aaaatatgct
     4381 gcttcgaaat taaaaggtgt tatttttcct atcagggcag ggattttcag gtttatcata
     4441 gtaaaaaatc ctggggctgc tctgggattt ttacgaaaga aacagtcttt cctgaaaatc
     4501 attaccctga ttatggttat ctttcttctt acatatacca ccctgtcact tatatataaa
     4561 gagcctgtaa aggacattct ttcgttggct ttaatatcgg gaggggcctt aggcaatttt
     4621 gttgatagga ttaggcataa atatgtaatt gatttcatca gcttgaatat aaaaagattc
     4681 cctgttttca acatggcgga tgtatttatt gcaactggtt ttgttctgta tattgtttag
     4741 agaagttatt gctgaagcta ttcagtaaga agatatttaa ggtgctttcg aacctttacg
     4801 cttactatgc ttgcaataca cacttttaat atatctccag ggataaaggc caaattggcc
     4861 aacaacagag gcaatgaaag gaaattgcct gtcctctcat aacaaagcca gggaatacct
     4921 attgcgtaaa ctacgattat accgcccatt gcacaggcaa cacctgttct caaaatattg
     4981 cagtcctttc ccttaagaag ggatattgta attgcaccta tgagaaaacc aataatgaat
     5041 ccgcctgtag gaccggataa atatgccaga cctgctccgc ctttactgaa cacaggaatg
     5101 cctatcgcac ccagcagaat aaaggtt
//
LOCUS       DAOOJC010000135         5119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12972_length_5119_cov_3.517378, whole genome shotgun sequence.
ACCESSION   DAOOJC010000135 DAOOJC010000000
VERSION     DAOOJC010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12972_length_5119_cov_3.517378"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLA01_05010"
     CDS             <1..220
                     /locus_tag="PLA01_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septation protein SpoVG family protein"
                     /protein_id="HPD00692.1"
                     /translation="NEFVVHDIKVIESQNGLFIAMPSRKAPDGEFRDIAHPINADTRG
                     KIQSAILDKYESISTNEAEGSQSETTEE"
     gene            701..2089
                     /gene="glmU"
                     /locus_tag="PLA01_05015"
     CDS             701..2089
                     /gene="glmU"
                     /locus_tag="PLA01_05015"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HPD00693.1"
                     /translation="MEQMMAVILAAGEGKRMKSKKAKVLHEICGMPLVKWVYQSARNA
                     GINDTVIVVGHKAQEVKEKLGDEILYAFQEKQLGTGHAVMQAQEHFADKEGYVIVLYG
                     DTPLITAETLKDAISFHKENNNSATVITADLDDPTGYGRIIRSGDGSVLRIVEHKDAS
                     DEEKNIKEVNSGMYCFNISDLREALGELDNNNSQGEYYLTDTIEILLNKGKNVKAFKI
                     KDSNEILGINDRIQLAKAGEIIRNRILQRFMENGVTIIDPASTFIDQEAEIGIDTVIY
                     PSTIIEGTTKIGEDCVIGPGSRIVSCEIADGVEVKNSVLLESSIDSGTKVGPFAYMRP
                     GSKIGKNVKIGDFVEVKKSIIGDKTKISHLTYVGDAEIGKGVNLGCGVVVVNYDGKKK
                     NKTVVGDNCFVGCNVNLISPVEVEDNAYIAAGSTITDLVPENALAIARSRQVIKENWV
                     VKKGMLKEDKKD"
     gene            2149..3111
                     /locus_tag="PLA01_05020"
     CDS             2149..3111
                     /locus_tag="PLA01_05020"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPD00694.1"
                     /translation="MNLHGKDIKIFAGNSNIELAKEIAEKMGLPLGMATVGKFSDGET
                     AININEVVRGSDVFIIQSTCPPVNDNLIELLIMIDAMKRASAGRITAVMPYFGYARQD
                     RKAKARDPISAKLVANLLTTAGADRVLTMDLHAPQLQGFFDIPLDHLLGGTLLAHYFL
                     EKFGDTTDVVVVSPDVGSVSRSRKFAQRLDVPLAIIDKRRPKANVSEIMNIIGDVNNK
                     RVILVDDIIDTSGTIVNAANALAEMGAKEVYACCTHGLLSGPAIERIKNSQMKELVTL
                     NTIPLTESKKIDKITALSVATVFAEAIERIYGDISISSLFTQKD"
     gene            3325..3906
                     /gene="pth"
                     /locus_tag="PLA01_05025"
     CDS             3325..3906
                     /gene="pth"
                     /locus_tag="PLA01_05025"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPD00695.1"
                     /translation="MEDLFIVIGLGNPGKRYENTRHNVGFDTVELLSRKHGILLTKLK
                     HKALLGDGKISGKRVILVKPQTYMNLSGESVREILEWYKVPVKNIIIVYDDIDLPVGK
                     LRLRPRGSAGTHNGMRSVIYQIESDEFPRIRIGVGGPPEGWDLADYVLSKFSAEDKKK
                     VEEAILNAADAVETILRSGIDMAMNRFNKNGTQ"
     gene            3930..>5119
                     /locus_tag="PLA01_05030"
     CDS             3930..>5119
                     /locus_tag="PLA01_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPD00696.1"
                     /translation="MGYFGVKHFLIGPLLEMNEYLSVLEDIKRGMPVTVTGPSESQKA
                     HMAYALCNHLGKKGVYIAYNELQARKIYEDFSCFFGRDVVLFTSREIMLHDVDAKSYD
                     SVYERIDTLYRIERGDYNFIVTSAEALCQKLVERELFVDSILSFSVGGRIDLEFTIQK
                     LISIGYERDTTVEGKGQFAVRGGIIDIFPVNSDSAIRIELFDDEIDSIRSFDLLTQRS
                     IENVEDVTVLPAKEVIYPYQRRDSILQKISNDLEEQIKGIKNHSRNKTAAQSNAADSK
                     SDIEKLQERIKFDIERFQQEYYFAGMDRYISYILENPFSVIDYIDSDVLTFVDEPNRF
                     ENRIEGFLEENYEMCKSLFEKGQLLKGSVEFFFDYEQLSNKLSGRKNLLYFCTLSADH
                     RQPG"
BASE COUNT         1698 a          771 c         1255 g         1395 t
ORIGIN      
        1 taatgaattt gttgttcatg acataaaggt tattgagagt caaaacggtt tattcattgc
       61 tatgcctagt agaaaagctc cagatggaga attcagggat attgctcatc ctatcaatgc
      121 tgataccagg ggcaaaatcc aaagtgcaat tttggataaa tatgagtcta tatctacaaa
      181 cgaagccgaa ggatcacaga gtgagacaac agaagaataa ttaattaatc caaaagaata
      241 cttagaaatg tttttcataa agctttttga atcgtgaaaa gtgttgcatt gcttttgcct
      301 tgtggctatt cattaaattg cggctggatc ggatgtttgc cggttgagtc atgaagaata
      361 tgccggagca gggtagaaag ggtttccttg tgtaccgtac aatatatttt tgttgtcgtc
      421 ggtaacaggg aggtcatgga cagatgctta cttttgacga ttttttatta tttttgcggg
      481 atttttttaa attaatttga tgttgaaaag ttactaaaaa aatgagaaaa tatatgtggc
      541 ttttagaagc agttatatgc aaactgatgt tggtataaca taacggatga atgcttcacc
      601 aataataaaa cctataaagg tatggacggg gtaattattt aagatcaggg gttggataag
      661 tgtttattct ttttgattga gctaaaggag ggaaaataat atggaacaaa tgatggcagt
      721 tattcttgcg gccggtgaag gaaaaagaat gaagtcaaaa aaggcaaagg tattgcatga
      781 aatttgtgga atgcctttgg ttaagtgggt atatcagtcg gcaaggaatg caggcataaa
      841 tgatacggtt attgttgtgg gacataaggc acaagaggta aaggagaagt tgggagacga
      901 aatcctttat gcttttcagg aaaaacagtt aggtacaggt catgcagtga tgcaggcaca
      961 ggaacatttt gcagataaag agggttatgt gattgtgtta tatggcgata ctccgcttat
     1021 taccgcagag actcttaagg atgcaatcag ttttcataag gaaaataaca attctgcaac
     1081 ggttataacc gcagaccttg atgatccgac aggatacgga aggattataa gaagcggtga
     1141 cggcagcgta ctccgaattg ttgaacacaa ggatgcatcg gatgaggaga aaaacataaa
     1201 agaagtcaat tcaggaatgt attgcttcaa tatcagtgac ctgcgagaag cacttggtga
     1261 gcttgacaat aacaacagtc agggagagta ttatctgacg gataccatag aaatattatt
     1321 aaataaagga aaaaacgtaa aagcatttaa gataaaggac agtaatgaga tactgggaat
     1381 aaatgataga atacaattgg cgaaagcagg cgaaataatt aggaacagaa tcttgcaaag
     1441 attcatggaa aacggtgtga caattattga tcctgcttca acttttattg atcaggaagc
     1501 cgaaattgga atcgatacgg ttatttatcc ttcaactatt attgagggaa caaccaaaat
     1561 cggagaagat tgtgtaatag gtcccggaag caggattgtg agttgtgaaa tagctgacgg
     1621 ggttgaggtc aaaaactctg ttttactgga aagctcaata gacagtggga caaaggttgg
     1681 accttttgca tatatgcgtc cgggaagcaa aatcggcaag aatgttaaaa tcggagactt
     1741 tgtagaggtt aaaaaatcaa taatcggaga taaaaccaag atatcgcacc ttacttatgt
     1801 tggtgatgca gaaataggca agggagtaaa ccttgggtgc ggggtggtag tggttaatta
     1861 tgacggaaag aaaaagaata aaacagtagt aggagacaac tgctttgtgg gctgtaatgt
     1921 aaacctgata tctcctgtgg aggtcgagga caatgcatat attgcagccg gttcaaccat
     1981 taccgacttg gtaccggaga atgctttggc tattgccaga agcaggcagg ttattaaaga
     2041 gaattgggtt gtaaaaaagg gtatgttgaa agaggataaa aaggattaac cgcataatat
     2101 taaattaaca taaaacagat aataatataa taagaagggg aacatgaaat gaatttacat
     2161 ggcaaggata ttaaaatttt tgcaggtaac tcaaatattg agttggctaa ggaaattgcg
     2221 gaaaagatgg gactcccgct gggaatggct actgtgggaa agtttagcga cggtgaaacc
     2281 gctattaata ttaatgaggt cgtcagggga tcggatgtgt ttattataca gtctacctgt
     2341 ccccccgtta atgacaacct gattgaactg ctgattatga tagatgcgat gaagagggct
     2401 tctgcaggaa gaatcaccgc cgtaatgccg tatttcggat atgcgaggca ggacagaaaa
     2461 gccaaagcaa gggatcccat ttcagcaaag ctggtagcaa atcttttaac aacagcagga
     2521 gcggacagag tacttacaat ggatcttcat gcacctcagt tgcagggctt ctttgacatt
     2581 ccgctggatc atttgctggg aggtacactt ttagcccatt acttcttaga aaagtttggg
     2641 gataccactg atgtggttgt tgtttccccg gatgtcggca gtgtttcgag atcaagaaaa
     2701 tttgcccaga ggctggatgt tcccctggct ataattgata aaagaaggcc taaggccaat
     2761 gtttcggaaa taatgaatat tattggggat gttaataata aaagagtaat tctagtggat
     2821 gacattattg acaccagcgg tacgattgtt aatgctgcca acgctctggc agaaatgggt
     2881 gcaaaagaag tgtacgcatg ttgtacccat ggtcttttgt ccgggcctgc aatagaaaga
     2941 attaaaaact ctcaaatgaa ggaacttgtt accttaaata caattccttt gactgagagc
     3001 aagaagatag ataaaattac tgctctttca gtagcaaccg tatttgccga agctattgag
     3061 aggatatatg gggatatctc tataagctct ctctttactc aaaaggatta attattgcct
     3121 gttagtggac aagtatagaa atttacatat atgccccctt aaattaatta agggggcata
     3181 ttaataatat taagcggtat ttcgcgaagt acagagaaag ataattgcat tatcggagca
     3241 gagtgcatta taaaaatgtg aagggaaaca ggaagaaact tcacaatgca caaaaaagac
     3301 agaaatacgg gaggggtgat ttgtctggag gatttattta ttgttatcgg cctgggcaat
     3361 ccgggtaaga gatatgaaaa tacaagacac aacgtaggct ttgatacggt tgaacttcta
     3421 tcaagaaagc atggtatact gcttaccaaa cttaaacata aagcacttct gggtgacggt
     3481 aaaatttcag ggaagagggt aatcctggtt aaaccgcaga cgtatatgaa cctcagcggg
     3541 gagagtgtcc gggaaatact tgaatggtat aaagttcccg taaaaaatat tataatagta
     3601 tatgatgaca tagacctgcc ggtaggtaaa ctcagattga ggccgagggg tagtgccggg
     3661 acccacaacg ggatgagatc ggtgatatac cagattgaat ccgatgaatt tcccaggata
     3721 aggattggtg tgggcggacc tcctgaagga tgggaccttg cggactatgt tttaagcaag
     3781 ttttccgctg aggataaaaa gaaggttgag gaagcaattt tgaatgcagc ggatgctgtt
     3841 gaaacaatac tcagatcggg tattgacatg gctatgaata gatttaacaa gaatggtaca
     3901 caatagcaaa gtcttatgag gttattgaaa tgggttattt tggagtaaaa cattttttaa
     3961 taggccctct tttggagatg aatgagtatt taagtgttct ggaagatata aaaagaggga
     4021 tgccggttac ggttacagga ccatcggagt ctcaaaaggc tcatatggca tatgctcttt
     4081 gtaatcatct tgggaaaaag ggtgtttaca tcgcttacaa cgagcttcag gccagaaaga
     4141 tatatgagga tttttcctgc tttttcggca gagatgtggt gctttttaca tcaagggaga
     4201 ttatgctcca tgatgtggat gccaaaagct atgattcggt atatgagaga attgatacat
     4261 tataccgtat tgagagaggg gactacaact ttatagtaac aagtgccgag gcgttatgcc
     4321 agaagcttgt tgaaagggag ctttttgttg acagcatact gagtttttct gtcggtggac
     4381 ggatagactt ggagtttaca attcaaaagc ttatatcgat agggtatgaa agagatacaa
     4441 ctgttgaggg gaaaggccaa tttgctgtaa ggggaggtat tattgacatt ttcccggtaa
     4501 acagcgattc ggcaattagg attgagcttt ttgacgatga aatagactcc ataagaagct
     4561 ttgatcttct aacccagaga tccatagaga atgtggagga tgttacggtc ttgccggcaa
     4621 aggaagtaat ctatccatac caacgcaggg actccattct gcagaagata tccaacgacc
     4681 ttgaagagca aatcaaagga ataaaaaatc atagcaggaa taagactgcg gcacaatcga
     4741 atgcagcaga cagcaaaagc gatattgaaa aacttcagga aagaataaaa ttcgatattg
     4801 agcggtttca gcaggagtac tattttgctg ggatggacag atacatttct tatatcctag
     4861 aaaacccctt ttcggtaatt gattacattg attccgatgt tttaaccttt gtggatgaac
     4921 ccaacaggtt tgaaaacagg attgaaggat ttcttgaaga aaactatgaa atgtgtaaaa
     4981 gcctttttga aaagggacag cttcttaaag ggagcgttga attctttttt gattatgaac
     5041 aactttcaaa caagctgagc gggagaaaaa atttattgta tttctgcacc ctatccgctg
     5101 accacaggca acccggcat
//
LOCUS       DAOOJC010000136         5119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12973_length_5119_cov_3.320103, whole genome shotgun sequence.
ACCESSION   DAOOJC010000136 DAOOJC010000000
VERSION     DAOOJC010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12973_length_5119_cov_3.320103"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2650)
                     /locus_tag="PLA01_05035"
     CDS             complement(<1..2650)
                     /locus_tag="PLA01_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPD00697.1"
                     /translation="MKVITKNKRIKVIVNGREMEVYDNLTILQALLQEDVHIPHLCYD
                     IRLERSNGNCGLCLVEVGEGEQKMDVKACQTPIREGMVITTNSPRLKSYRKIRLEQIL
                     SDHNADCVAPCVMTCPDNIDIQAYLHHAANGNFSAALQVIKDRNPFPVTCGRVCPHPC
                     EAQCRRNLVDSPVAINHVKRFIADWDMSQEEPWMPQKKDHTGKRIAIVGAGPSGLTCA
                     YYSAINGHDVTVFERQHYAGGMMRYGIPEYRLPKATLDKEIDIIKDLGVMIMTKKALG
                     THIRLEDLQKDFDAVYLAIGSWRATPMHIEGENLPGVWLGIQFLEQVTKQADVQLGDN
                     VAVIGGGNTAIDCARVAKRLGAKSVKLIYRRTRDEMPAEPYEVEEALHEGVEMLFLMA
                     PTNIVLENGKKKLQCIRMELGEPDRSGRRRPVPIEGTDFEIDADTIIGAIGQSTNTQF
                     LYNDLPVKLNKWGDIEINGKTSQTSEVNIFAGGDCVTGPATVIQAVAAGRRAAEAMDS
                     FLMKGYVQESHVDYSCSRGSLEDLPKWDFEHFPRLKRAKMPSIELKEREGNFNEVELG
                     LSEEAAREEAHRCLRCGCNKRYDCDLRTEASAHDIKFENPIHERPYIPIVDDHPFIIR
                     DHNKCISCGRCIAACAQVEGPDVLAFYMKQGRQLVGTRSGLPLQETDCVSCGQCVNAC
                     PCGALEAKSEKGKVFRAINDHSKTVVAFVAPAVRSVISSHYGIPFDKASGFMAGLLKN
                     IGFDKVFDFSFAADLTIVEETTEFLNRLDNKGVMPQFTSCCPGWVNFVERRYPELIPH
                     LSTCKSPQQMMGATVKNHYTKLAGLNKEDLYVVSIVPCIAKKYEAARPEFAPDGIRDV
                     DAVLISSDMIDMAELKRIDTSSIELQE"
     gene            3058..4203
                     /locus_tag="PLA01_05040"
     CDS             3058..4203
                     /locus_tag="PLA01_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00698.1"
                     /translation="MTITTFAASPDATLIVDGIEVQTFSSVQGAVDAIDSTGGNNFVI
                     EIAEGTVSDVVYILQIPDKNIVLRPKPGASVTFTNTITIDGNSNYYGSETLLIQGFNF
                     DFTSGAFENCIHFALVPSRAAMDPPPLSYPHNITINGCTFKGVYGTTVAVQSLRGGSR
                     NIAIMNCTATDIHSFAQLKAVAGYAFIQNCIVSNASEGGVNFYGTGDLTVDSCKFDVV
                     GYAVRSGQNDPTLAPILQGSVNINNSILNSNSSEFGTIVLRGESSNNINILHSILKNE
                     SAGGAVIENLYSDYEDEYDIDVVESDLTGEITNINLSTITTIDDPNVPNGPVSVTDEP
                     ENPFRDLLLLILSIPILVIILVILLLVLVILIVRLIIRFIRFLINLI"
     gene            4418..4546
                     /locus_tag="PLA01_05045"
     CDS             4418..4546
                     /locus_tag="PLA01_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00699.1"
                     /translation="MEEDRGFGCGVGNDFIWIIFIILIILCLCPGIFGGFGFGCKN"
     gene            complement(4732..4932)
                     /locus_tag="PLA01_05050"
     CDS             complement(4732..4932)
                     /locus_tag="PLA01_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00700.1"
                     /translation="MESAQRNKELITKFNEIQEEILRIGWNGIIQKYHPDMNCNVANS
                     AKVFKMYKSIYENMKKRVIVKT"
BASE COUNT         1454 a         1046 c         1056 g         1563 t
ORIGIN      
        1 attcctgaag ttcaatggac gatgtatcaa tacgcttaag ctcagccata tctatcatgt
       61 cggaagagat cagaaccgca tctacatcac ggattccatc gggtgcaaat tcaggacggg
      121 cagcctcgta ttttttagca atacaaggaa cgatggacac cacgtacaga tcttccttat
      181 tgagacctgc cagcttggtg taatgatttt tcacggtagc acccatcatc tgctgcggtg
      241 atttacaggt agacagatgg ggaatgagtt ccggataacg ccgttcaacg aagtttaccc
      301 atccggggca gcaggaagta aactgcggca taacaccctt attatctaaa cggttcagaa
      361 attcggtggt ttcctccaca atggttaagt cagcagcaaa ggaaaagtcg aagactttat
      421 cgaaaccgat attcttcaaa aggccagcca taaagccgga agccttatca aagggaatac
      481 cgtagtggga ggaaatgacg cttcgtacag cgggtgcgac aaaggcaacc actgtcttgg
      541 aatgatcatt gatggctctg aagactttgc ctttttcact cttcgcttcc agtgcaccgc
      601 agggacaagc atttacacat tgtccgcagc tgacacaatc ggtttcctga aggggaagac
      661 cacttctggt tcctaccaac tgacggccct gcttcatata aaaggcgagg acatcgggac
      721 cttcaacctg tgcacaggcg gcaatacaac ggccgcagga aatacatttg ttgtggtctc
      781 tgatgataaa tggatggtcg tctacaatag gaatataggg tctttcatga atcggatttt
      841 caaatttaat atcatgggca gatgcctcag tccttaaatc gcagtcatag cgtttgttac
      901 atccgcatct taagcagcgg tgggcttcct cacgagcagc ttcttcggaa aggcccagtt
      961 caacttcatt aaaattacct tctctttctt taagctcaat agaaggcatt ttagcacgct
     1021 tcagtctggg gaaatgctca aaatcccatt tcggtagatc ctccagggag cctctgctgc
     1081 aactgtaatc cacatggctc tcctgaacat atcccttcat gaggaagctg tccatagctt
     1141 cggcagcacg acggccggcg gctacagcct gaatgactgt tgcagggcct gtcacacagt
     1201 cccctccggc gaaaatattg acttctgagg tctgtgaggt cttaccgttt atctcaatat
     1261 ctccccattt gttcagctta acagggaggt cgttatacag gaattgtgta ttcgtacttt
     1321 gaccgattgc accgattatg gtatcggcat ctatttcaaa atcggtacct tcaatgggta
     1381 caggacggcg acggcctgaa cggtcaggtt ctccgagctc cattctgatg cattgcagct
     1441 tcttctttcc gttttctaaa acaatatttg taggtgccat taaaaagagc atctcaaccc
     1501 cttcatgaag tgcctcttca acttcataag gttcagcagg catttcgtca cgggttctgc
     1561 ggtagataag cttaaccgat ttagccccca atcttttggc aacgcgggca cagtcaatgg
     1621 cagtgttgcc tccgccgatg acagcaacat tgtcacccaa ctgaacatcc gcttgcttgg
     1681 tgacctgttc taaaaattgt ataccaagcc atacacccgg gagattttct ccttcaatat
     1741 gcatcggggt agcacgccat gaaccgatgg ccagatagac ggcatcaaaa tccttctgga
     1801 gatcttcaag acggatatga gtacccagag ccttttttgt cataatcatt acgccaaggt
     1861 cttttataat gtcgatttcc ttgtcaagag tcgccttcgg cagacgatac tcgggaatcc
     1921 cataacgcat catacctccg gcataatgct gacgttcaaa taccgtaacg tcgtggccgt
     1981 tgatggcact gtaatatgca cacgtaagac ctgacggtcc ggcacctaca atggcgatac
     2041 gcttcccggt atggtctttc ttttgaggca tccaaggctc ttcctgagac atatcccagt
     2101 cagcaatgaa acgctttacg tgattgatag ccacgggaga atcgaccaaa ttgcggcggc
     2161 attgggcctc acagggatgc gggcatacac ggccacaggt aacaggaaac ggattgcggt
     2221 ctttgattac ctgaagagcc gcactgaaat tgccgtttgc tgcatgatgc agataagcct
     2281 gaatatcgat attgtccgga caggtcatga cacaaggtgc gacacagtcc gcattgtgat
     2341 ccgataggat ctgctccagg cggatcttac ggtagctttt tagtctgggg ctgttggtgg
     2401 tgattaccat accctcccgg ataggtgtct gacacgcttt tacatccatt ttttgttcgc
     2461 cttcgcctac ctctaccagg cacaatccac agtttccgtt tgatcgctca agacggatat
     2521 catagcacag atggggaatg tgtacatcct cttggagcaa ggcctggagg atggtcagat
     2581 tatcatagac ctccatttcc cggccgttaa caataacttt aatccgtttg ttttttgtga
     2641 tgactttcat tttattaaac tccttcttat gatgtgtgat atttttcgtg atgaaacaac
     2701 aatagaaccg tatcaattat atcagaaata ttgttattta ccactacttc aacgtcataa
     2761 aatcgctttt tttaacaaaa aaatttagtt tttttgatac ttcctatacc tttcttgctt
     2821 tatcaaaggt aagcgtaatt cattttttca tcacttattt atgttaagct gtcgaaagga
     2881 atacggctga tgaaaatata gcacgctaag gtgaatcggt aataaggaac atgatgggtt
     2941 tagtcagcat attatattat ggaaactaaa taataaaccg ggaggttaat tatgaaaact
     3001 aagaatattt ttatttttgc atcaattata tgcttgtgtt tcctatgctt atgttcaatg
     3061 acaattacaa catttgcggc ttctcccgat gcgacactta tagtagacgg aattgaggta
     3121 caaacttttt catctgtaca aggggctgta gatgcgattg attctaccgg cggaaataac
     3181 tttgttattg aaatagcaga gggaacggtt agcgatgtag tttatatttt gcagataccg
     3241 gacaaaaata tagttttgcg accgaagcct ggggcatctg tcacatttac aaacacaatt
     3301 acgattgatg gaaactcaaa ttattacggt tcagaaactt tgctgattca aggctttaat
     3361 tttgatttta cctcaggagc tttcgagaac tgtattcatt ttgctttagt accatcaaga
     3421 gcagcaatgg atccaccacc attaagctat cctcataaca tcaccattaa cggttgtact
     3481 tttaaaggcg tttatggcac aacagtggcg gttcaaagtt tgcgtggcgg ttcaagaaat
     3541 attgcaataa tgaattgtac cgctaccgac attcacagtt ttgcacaatt aaaggctgta
     3601 gcgggttatg cattcattca aaactgtata gtaagcaatg cttctgaggg cggagtaaac
     3661 ttctatggaa ctggagactt aactgttgac agctgtaaat ttgatgtagt gggctatgcg
     3721 gttcgcagcg gacaaaatga tcctactttg gctcctatat tacagggctc tgtaaatatc
     3781 aataacagca tattaaattc aaattcatcg gaattcggta caattgtttt gagaggcgaa
     3841 agctctaata atatcaatat tttgcattca attttaaaaa acgagagtgc cggtggagcg
     3901 gtcatagaaa acttatattc ggattatgag gatgaatacg atattgatgt tgtagagtcg
     3961 gatctgacag gagaaattac caatattaat ctttcaacca taactaccat cgatgatcca
     4021 aacgtgccca atggacctgt ttccgtaact gatgaacccg aaaatccctt tagggactta
     4081 cttcttttaa tattatcaat tccgattttg gttataattt tggtaattct gctgctggtt
     4141 ttggttatct tgattgttag actcattatt agatttatta gattccttat caatctaatt
     4201 taataattaa atactcctga aatgaaacga tagcgaaaga tgcagttaaa ctaaataaag
     4261 atagcactca ccaaaaggtg ggtgctgttt ttataataaa tctttattga atctaaggta
     4321 tttttttgta tgataataaa tctaaatatt gtaacacttt ttgagcttta gaatattata
     4381 gtactgttga taaataaaaa tgaaagggtg gatttgtatg gaagaagata gaggttttgg
     4441 ctgtggggtt ggcaatgatt ttatatggat aatctttatt attttgatta tcctgtgtct
     4501 gtgtcccgga attttcggcg gttttggatt cggatgcaaa aactgatacc ttaataaatc
     4561 ttgctccagg cttgttttga aggagacctt taaacttaca tattgaacag cttgaaaaag
     4621 aaagaggaga cttttcttct ctttcttttt aatcagtggg atttcacaaa gtgaaatgac
     4681 caaaaacaaa tggttgaaca aagttcaacc atctaaatgc tgatattact attatgtttt
     4741 aacaataaca cgttttttca tattttcata tatgcttttg tacattttga agactttagc
     4801 agaatttgcc acgttacagt tcatatcagg gtgatacttt tgaattatgc cgttccaacc
     4861 aatccttagt atttcctcct gaatttcatt aaacttagta atcagctctt tgttgcgttg
     4921 tgctgattcc ataaaaactc ccccttaaaa aaatgcttta tataaatatc atctaaaacg
     4981 ttagttatgt agtttatttt attgggctga taattaaatt atagcactag ttttaattaa
     5041 tatcaagata gctgtgtgat ggtatcacgg taaaagcatg aacccatctc ctgccaatac
     5101 tctcacaaga gagtttttt
//
LOCUS       DAOOJC010000137         5116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_12978_length_5116_cov_3.882632, whole genome shotgun sequence.
ACCESSION   DAOOJC010000137 DAOOJC010000000
VERSION     DAOOJC010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12978_length_5116_cov_3.882632"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /gene="flgK"
                     /locus_tag="PLA01_05055"
     CDS             <1..1102
                     /gene="flgK"
                     /locus_tag="PLA01_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HPD00701.1"
                     /translation="SKESDSLTVRALVRQRAEALVYHVNHVGHQLDKLQNDLNYEVYM
                     RIGEVNDITSQIASLNKAIYSAEVTGDNANDFRDQRNLLIDRLSKLADFEATEMMDGQ
                     VDITLAGHYLVHKGEFMELYAGERNPGDIFYVPKLAGTNTEVSVKSGEIKALLDSRGD
                     SVNSLGSMDGGISALEDSTSIIPDLKRRLNAVINKMAEELNNLHRSGKTLSVPPEDGQ
                     DFFVPINPAYPVRFGNIQLNSNLIDLNNIVASKNGDSGDNSIALDIANLRNIQLIDDI
                     DRILSIDDAYQFIILGVGSGGAEAERMSQSQSHLVLSTDLQRNSIMGVSMDEEMSNMM
                     KFKFAYTASSRAINVIDEMIETIITKMGIVGR"
     gene            1109..2662
                     /gene="flgK"
                     /locus_tag="PLA01_05060"
     CDS             1109..2662
                     /gene="flgK"
                     /locus_tag="PLA01_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HPD00702.1"
                     /translation="MNPGFFGLEIAVRGLYTAQRNLDVINHNVNNINTPGYSRQIANQ
                     SASRPVALFNGTGMLGTGSDVTGINRIRDEYLDFKYWSENTTYGEWECKRALMADLEA
                     TFNEPSNSGFSTMLDEFFNSLQELAKDPGSEAVRALVKERGITLAKYFNNMATHMEEL
                     QVDLNHQINTKVNRINSLGSQIQQLNKQIYRSELDGNVANDLRDQRTLLIDELSKLVN
                     VDVHEVVAGKLPNGKEDKRMLITINGKPFVDHYDLTKLAVKQRADKLNPDEDVVNLYE
                     VMWEDGNSIQIRGGELKGLLDVRDGNEGENGSPLYKGIPFYIKKLNQFVRVFAQAFNE
                     GIIGGVDMEGHADGIGADNSTEIRFFTMLGTGNLPMSSSDFISNPDCYNELTAKNFCV
                     SSDIINDISKIATSDTPDQIGNIENINKLIKMRNNTHMFTEGAPEDFMKSIVSTLGID
                     SQQAVRMSANQNAMIRQVENQRLSESGVSMDEEMANMVKHQQAYNAAAIMINTMSEIY
                     DILINRVGL"
     gene            2716..3624
                     /gene="flgL"
                     /locus_tag="PLA01_05065"
     CDS             2716..3624
                     /gene="flgL"
                     /locus_tag="PLA01_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HPD00703.1"
                     /translation="MRITNSMLVNNMISNLGKNLSRMESYQQKLATGKKISVPSDDPV
                     VAARALKLRTDVSQIEQYKKNVKDAMSWLEVTESAIADVGDILQRARELAVQASNGAT
                     TPEDTKKICEEVKQLKTQLVNLGNATYAGRQIFSGFKTNQKLLNDDGSFAIDVDNTEG
                     IIYQIGVADSININVTGGDFFNSGNTATTGTFGTIFEDMNNFITALDSGDYAVIDETI
                     GKIDLQQNNLLRIRADIGARYNRLELTNNRLGNDGLNFTKLMSENEDIDQAENIMLLK
                     SEENVYRASLAGGARIIQPSLVDFLR"
     gene            3660..4238
                     /locus_tag="PLA01_05070"
     CDS             3660..4238
                     /locus_tag="PLA01_05070"
                     /inference="COORDINATES: protein motif:HMM:NF039791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6470 family protein"
                     /protein_id="HPD00704.1"
                     /translation="MGIRITQTYPKIGVDTVAADLNMKAHKPELEIHQNHSKLKIETE
                     FPKVEIDQYEAFASAGLKGPIDITHEIAQRIYQQVIEQIGKISEDGDIFAAIENGGNP
                     IADMAESDSVTEHEFDVDCIPKARPQVTVKGSQQIEAGREGEGVKNGVEFSITPGQIE
                     YNYIPAQVNIFLRQYGSVKIGYVQDNKVDVYI"
     gene            4275..4754
                     /gene="fliW"
                     /locus_tag="PLA01_05075"
     CDS             4275..4754
                     /gene="fliW"
                     /locus_tag="PLA01_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HPD00705.1"
                     /translation="MLLKTKHFDEIEIEEEKIINFKDGIPGFEEVKKYIVLYDGDENS
                     PFRWLQSVDDGQLAFAVADPFMIVNNYDIEIPQEAVDVLEIQSPEHVMILSILVVPED
                     FSKISMNLKAPVIINTKNKRGMQVVLDTDRYSVRHYIVEELRRREVNISAGSHKKEE"
     gene            4726..4941
                     /gene="csrA"
                     /locus_tag="PLA01_05080"
     CDS             4726..4941
                     /gene="csrA"
                     /locus_tag="PLA01_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HPD00706.1"
                     /translation="MLVLTRKKNESIVLNDNIEITIVDVQGDQVRVGINAPKSVSIYR
                     KEIFLEIQAENKKAADVKSVDLKNLLK"
BASE COUNT         1673 a          866 c         1272 g         1305 t
ORIGIN      
        1 ttcgaaggaa tcggacagtc ttacggtgag agcccttgtg aggcaaagag ccgaagcttt
       61 agtataccat gttaaccatg tcggccatca gctggacaag ctgcaaaatg acttgaacta
      121 tgaggtttac atgcgcatag gtgaggtcaa tgacataaca agtcagatag catccttaaa
      181 caaagcaata tattcagctg aggtaaccgg ggataatgcc aatgacttcc gggatcaaag
      241 gaatcttctt atagacaggc tttcaaagct tgcagatttt gaggcaaccg aaatgatgga
      301 cgggcaagtt gatataactc ttgccgggca ttatctggtg cataaaggtg agttcatgga
      361 gttatacgcc ggggaaagaa acccggggga tattttttat gttccaaagc ttgcggggac
      421 aaatactgag gtttcggtta agagcggtga aataaaagca cttttagact caaggggtga
      481 ctctgttaac tccctcggaa gcatggacgg cggaatcagt gctctagagg actccaccag
      541 tattatacct gaccttaaga gaagactcaa tgccgttatt aataaaatgg cagaagaatt
      601 aaataactta cataggagcg gaaagactct cagtgtccct ccggaagacg gacaggactt
      661 ttttgtgccg ataaatcctg catatcctgt ccgattcgga aatatacagc taaacagcaa
      721 tcttatagac ttaaacaaca ttgttgcatc gaaaaacggt gacagcgggg ataatagcat
      781 tgctcttgac attgcaaact taaggaatat ccaattaatc gatgatatcg acaggatatt
      841 gagcatagat gatgcttacc agttcataat attaggagtc ggcagtggag gagctgaggc
      901 agagagaatg agtcagagtc agagccatct tgttctttct accgacttgc agagaaactc
      961 cattatgggg gtttccatgg atgaggaaat gtccaacatg atgaagttta agtttgccta
     1021 tacagcatct tcaagggcaa taaatgttat agatgaaatg attgagacaa ttataaccaa
     1081 aatgggaata gtgggcaggt gatgaattgt gaatccaggc ttttttggat tggaaattgc
     1141 ggttaggggt ctttatacgg cacaaagaaa tcttgatgta ataaaccata atgttaacaa
     1201 tatcaatacc cccggctact caaggcagat tgccaatcag tcggcttcaa gaccggtagc
     1261 tctctttaat ggaaccggaa tgctgggtac gggttcggac gttaccggta tcaacagaat
     1321 aagggatgag tatctggatt ttaaatactg gagcgaaaac accacatatg gtgaatggga
     1381 atgcaaaagg gctttgatgg cggatctgga ggctaccttc aatgaaccgt cgaacagtgg
     1441 cttcagtacg atgctggatg agtttttcaa ctctcttcag gagcttgcca aggacccggg
     1501 aagtgaagcg gtaagagctt tggtaaaaga gagggggatt acccttgcaa agtatttcaa
     1561 caacatggca acccacatgg aagagcttca agtggatttg aaccatcaga ttaacacaaa
     1621 ggttaacagg atcaattcgt tgggctccca gattcaacag cttaacaagc agatataccg
     1681 ctcggagctt gacggaaatg ttgccaatga ccttagggac caaaggacgc tcttaataga
     1741 cgagctctca aaattggtca atgttgatgt gcatgaagtg gttgctggga agctccccaa
     1801 cggtaaagaa gacaagagaa tgctcataac aataaacggc aagccctttg ttgaccatta
     1861 tgatcttacc aagttggctg tgaagcaaag ggcggacaag ttaaaccccg atgaggatgt
     1921 ggtaaacctc tatgaagtga tgtgggaaga cgggaacagc attcaaataa gaggtggaga
     1981 gctaaaggga ttgttggatg tacgggacgg aaacgaggga gaaaacggaa gtccgctgta
     2041 taaaggcata cccttctaca taaagaagct gaaccagttt gtacgggtct ttgcccaggc
     2101 cttcaatgag gggataatag gcggtgtgga tatggaagga catgcggacg gtattggggc
     2161 tgacaactca acagaaataa ggttttttac catgctgggt acgggtaatt tgcctatgag
     2221 cagcagcgat ttcatcagta atcccgactg ctataatgaa cttacggcaa agaacttttg
     2281 tgtaagcagc gatataataa atgatataag taagattgct acctccgata ctccggatca
     2341 gatcggtaac atagagaaca taaacaaact tataaaaatg agaaacaaca cccatatgtt
     2401 taccgaaggg gcacctgagg attttatgaa atccatagtc tcgaccctgg ggatagattc
     2461 gcagcaggca gttaggatgt cggcaaacca gaatgccatg ataagacagg ttgaaaatca
     2521 gagactgtca gaatcgggag tatccatgga tgaggaaatg gcaaacatgg tcaaacacca
     2581 gcaggcgtat aatgctgctg caattatgat taatacgatg tccgaaattt atgacatatt
     2641 gataaacagg gtaggattat aagtgtagtt ttatagtatt gaacaacact tattagtaca
     2701 gtgtggaggg atgatatgag aataaccaac agtatgcttg ttaataacat gatatcaaac
     2761 ttgggcaaga atctgtcaag gatggaaagt tatcagcaga agcttgccac cgggaagaag
     2821 atttcggttc cgtctgatga ccctgttgtt gcggccaggg cattgaaact tcgtaccgat
     2881 gtgtcacaga tagagcagta taagaaaaat gtaaaggatg caatgtcgtg gctggaagta
     2941 acagaatcgg ccattgccga tgttggagat attctgcaaa gagcccggga attggcggta
     3001 caggcaagca atggggcaac cacaccggag gataccaaga aaatttgtga agaggtaaag
     3061 cagcttaaga cccagttggt caatctgggc aatgcaacct atgcaggaag acaaattttc
     3121 tcgggtttta aaaccaatca aaaactgtta aatgatgacg gttcctttgc cattgatgtt
     3181 gataatactg agggaataat ttatcagata ggtgttgcag atagtataaa cattaatgtg
     3241 acaggagggg acttttttaa cagtggcaat accgcgacaa ccggaacatt cggaacaatt
     3301 tttgaggata tgaataattt tataacggca ctggatagcg gtgattatgc tgtgattgat
     3361 gaaactatag gaaagattga cctgcagcaa aacaacctgc ttcgcataag ggctgacata
     3421 ggggccaggt acaacaggct ggaacttacc aacaacaggc ttggaaacga tgggcttaat
     3481 tttaccaagc taatgagtga aaatgaggat attgaccagg cggaaaacat catgctgcta
     3541 aagagtgagg aaaatgtata cagggcttct ttagcaggag gggccagaat aattcaaccg
     3601 tctcttgtgg atttcttaag gtaatataaa gtaataataa gtatagtgcg ggtggtttta
     3661 tgggtataag gataactcaa acctatccta aaatcggagt tgacacggtt gcggccgatc
     3721 ttaacatgaa ggctcataag cctgagcttg agattcacca gaaccattcg aagttaaaga
     3781 tagagacgga atttccaaag gtggaaattg accagtacga ggcgtttgcc agtgccggtt
     3841 tgaaaggccc cattgacatt actcatgaga ttgcacaaag aatttatcaa caggtaattg
     3901 agcagatagg gaaaatttct gaggacgggg atatctttgc ggctatagaa aacggaggaa
     3961 accccattgc agatatggca gagtcggatt cggttacaga gcatgagttt gatgttgact
     4021 gtatacccaa ggcaagaccg caggtaactg ttaagggatc acagcagatt gaagccggaa
     4081 gagaaggcga aggggtgaaa aatggtgtag agttttcgat tacccccgga cagattgaat
     4141 ataattacat ccctgcccag gtcaatattt ttctaaggca gtatggatcg gttaaaatcg
     4201 gttatgttca agacaacaag gttgatgtat atatataaac ataggaaggg aacttattaa
     4261 atggaggaga ttaaatgctt ttaaaaacaa agcactttga cgaaattgag attgaggaag
     4321 aaaagataat taattttaaa gacggaattc ccggttttga ggaagtaaaa aagtatatcg
     4381 tgctgtatga cggggatgag aattctcctt tcagatggct tcagtcggta gacgatggac
     4441 aattggcttt tgcagttgct gacccgttta tgattgtcaa caactatgac atagaaattc
     4501 cgcaggaagc cgtggatgtt cttgaaattc aaagtcctga gcatgttatg atattgtcta
     4561 ttcttgtagt accggaggat ttttctaaaa tcagtatgaa tttaaaagca ccggttatta
     4621 ttaatacaaa aaataagaga ggaatgcagg ttgtccttga cacggataga tattctgtga
     4681 gacactatat cgtagaagaa ctccgcagac gggaggtaaa tattagtgct ggttctcaca
     4741 agaaagaaga atgaatctat tgttttaaat gacaatattg aaattactat tgttgatgtt
     4801 cagggagatc aagtgcgtgt aggcattaat gctccaaaga gtgtttctat ttatagaaaa
     4861 gagatatttt tggaaataca ggctgagaac aaaaaggccg cagatgtaaa gtcggtagac
     4921 ctgaagaatt tattgaaata aaacaacaca agggaacgat tctcttgatt aaatatcatt
     4981 ccactgatac tgcatatcgg aggcatgatt tttttatgcc tacttttaga acaggggact
     5041 gttctcagtc taattctggc ggtttgaaat atacagtata ttttatcaat attatatttg
     5101 caggatttag aagaaa
//
LOCUS       DAOOJC010000138         5101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_13030_length_5101_cov_4.181728, whole genome shotgun sequence.
ACCESSION   DAOOJC010000138 DAOOJC010000000
VERSION     DAOOJC010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13030_length_5101_cov_4.181728"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="PLA01_05085"
     CDS             complement(<1..1370)
                     /locus_tag="PLA01_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbfB domain-containing protein"
                     /protein_id="HPD00707.1"
                     /translation="MKYQGVILKLTKNKAIVTTDDFQCYYIKRRPTIYVGKEVEFTSG
                     EILGRRTVLIKPLLSVACFLIVITSLLYFSGIINLNNVLFNPKVFAYVGVDINPSLEI
                     EIDDAGNVLNLVPLNDDAKALADKLKIGRINVSKAIDNIIDEVKKNSAVNESEKDCVL
                     ISSTLNRKKGENDKESQRKKEKLNSIVNSLKDNIQKSGKADVYIVQADIAEREAARIE
                     GISTGRYVLYNKYKDLGSGFSLEEAKKANVNDLIKSVLGNGSVKGKPEETPTVAPTPT
                     ATPIPTSTVTPTPISTPTPTSKQTPVPTATLTSKPTLTPTSTPTATPTPTPKPTPTPK
                     PTPAPKPTPKSTPVPKSTPTSTQKQTPTPTSTLKPTPTLTPTPTATPTPTPTPTVTSI
                     PTAKTTGSGFVRLESSNYRGYYIRVKSFTARIDPYVEPVEDSIFKIVPGLADPSCVSF
                     ESKNHPG"
     gene            complement(1472..2209)
                     /locus_tag="PLA01_05090"
     CDS             complement(1472..2209)
                     /locus_tag="PLA01_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPD00708.1"
                     /translation="MLFISAIVDYTSETATRIVEKIKDGDKYLKEKFIKDYIPFILNI
                     VSGFYSSKMVDLKSSDEYSIGLMAFNEAIEKFDISRSRSFLKFAEMVIKKRMIDYFRK
                     TSSISRKEIPFSYFSNNNESEFEKKLNMADLDQESGSYEFICEIRDFSKQLESFGLNI
                     VNLPDYMPKHKDSRQMCIDIARKIIENKDILNKLKTKKYIHMKELSKIIDVHPKTVER
                     NRAFIICLCILLDNDYANFKAYLNKIF"
     gene            complement(2362..4392)
                     /locus_tag="PLA01_05095"
     CDS             complement(2362..4392)
                     /locus_tag="PLA01_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPD00709.1"
                     /translation="MVKKTGRVWLIALIVCFLALLLPEKGFADYPIFSQRYTADPTAV
                     VYNGRLYVYCSHDSDVTPGQSTYNIPDITCISTDDLKNWTDHGEVFNAKRDSQWAGVS
                     WAPSIVYRNNKFYMYYGNGGNGIGVAVSDSPTGPFKDPLPKALITGNTSGVQPAPNMW
                     LFDPGVFIDDDGQAYMYFGGNGENNIRVIKLGSDMISTVGSAMSMSAPRFFEASFMHK
                     YKGKYYFTYASDFSQGASKIEYMMSDSPTSGFTYKGVVLPQPPDNYNNNNHHTIVEFK
                     GNWYCVYHNRTVAKQRGEDPVYQRNVCIDQLFYNSDGTIKQVVPTVDGLKQLKYVDPY
                     AENTAVTMHKESGIETEECSEGGRNVAFIENGDWIQIKGVDFGSGGATSFEARVASNT
                     EGGNIEVRLDGPTGTLVGTCKVEGTGGWQKWTTKSCSISGVTGVHDLFFRFTGGSGYL
                     FNFSWWKFDSDTIPTPTTPPTPSPTPVVERSAFSRIEAEEYNDINSSTMQIIGTAGGG
                     SGIGYIENGDYLVYKSIDFGNGATVFKALVATTGTPNIELRLNSPTGTLIGTLPVAST
                     GGFNAYEEQSCSVSKVTGKHDLYLKFSGAVNIDWFTFDEDGGAGRKGDVNGDGEIDST
                     DVTILKRYLLKKMSLSGENLSNADTNGDGEVNSTDLTLLKRYILGKIASFPV"
     gene            complement(4865..>5101)
                     /locus_tag="PLA01_05100"
     CDS             complement(4865..>5101)
                     /locus_tag="PLA01_05100"
                     /inference="COORDINATES: protein motif:HMM:NF022611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2947 family protein"
                     /protein_id="HPD00710.1"
                     /translation="VNFLQDNILISKEAIITFFWSESCSVETTWGVFLKYWTDFCYPS
                     DDSNVIIIHDYPLAVIYIEDKLWIVNRAEYFGSC"
BASE COUNT         1412 a         1073 c          863 g         1753 t
ORIGIN      
        1 ccaggatgat tcttcgattc aaaagaaaca caactcgggt ctgcaagtcc gggaactatc
       61 ttaaatatag aatcttcaac tggctccaca tacgggtcaa tacgtgcagt aaatgattta
      121 acccgtatat aatacccgcg ataattactg gattcaagtc taacaaaccc tgaacctgtg
      181 gttttagcgg ttggtattga tgttaccgtt ggtgttggcg ttggtgtcgg tgttgctgtt
      241 ggtgtaggtg tcaatgttgg tgttggtttt agtgttgacg ttggcgtcgg tgtttgtttt
      301 tgtgttgatg ttggcgtcga tttcggtact ggtgtcgatt ttggtgttgg ttttggtgcc
      361 ggtgtcggtt ttggtgtagg cgttggcttt ggtgttggtg tcggtgttgc cgttggtgtt
      421 gatgtaggtg tcagtgttgg tttggatgtc agtgttgccg ttggtaccgg tgtttgcttt
      481 gatgttggtg tcggtgttga tatcggtgtt ggcgttaccg ttgatgtcgg tataggtgtt
      541 gctgttggtg ttggtgctac cgttggtgtt tcttcaggct ttccttttac tgatccattc
      601 cctaatacac tctttatcaa atcattgaca ttagccttct tagcttcctc aagtgaaaaa
      661 ccgcttccta aatccttgta tttattatat aaaacatatc tgccggtaga tatcccttca
      721 attcgtgctg cctctctttc ggctatatcg gcttggacga tatatacatc ggctttgccg
      781 ctcttttgta tattatcctt cagtgaattc acaatagaat taagtttttc cttttttctc
      841 tgggattcct tgtcattttc cccttttttt ctgtttaatg tgcttgaaat caatacacaa
      901 tctttctcag actcatttac ggcactattc ttttttactt catctattat attatcaata
      961 gctttagaaa cattgattct gccgatcttt aatttatctg caagagcctt tgcatcatca
     1021 tttagcggaa ccaaattcaa aacatttccg gcatcatcaa tttcaatctc aaggctggga
     1081 tttatgtcga ctccgacata agcaaaaacc tttggattga ataagacatt atttaaatta
     1141 attattcccg aaaagtataa taaactggtt attacaataa gaaagcaagc aacacttaaa
     1201 agcggtttaa ttaaaaccgt ccgccttcca aggatttctc cactggtaaa ttcgacttcc
     1261 tttcccacat aaattgtagg tctcctcttg atatagtagc actgaaaatc atcagtagtt
     1321 actattgctt tgttttttgt cagttttaat ataacacctt ggtatttcat tgctatcctc
     1381 caaaaagtgc agattacata cgataaaccg tatgcctgcc aacttttaaa tttattcgtg
     1441 tttataagaa tttttatgct ctttcaatac attagaatat tttgtttaaa tatgctttga
     1501 agtttgcata atcattgtca agcagtatgc atagacatat aataaatgcc ctgtttcttt
     1561 caacagtctt cggatgcaca tcgattattt tcgaaagctc tttcatatgt atgtactttt
     1621 tcgtttttaa cttgtttaag atatccttgt tttcaattat ttttctggct atgtctatgc
     1681 acatctgtct tgagtccttg tgtttcggca tatagtcagg caaattgact atatttagac
     1741 caaagctctc cagctgcttc gaaaaatccc taatttcgca aataaattcg taactacctg
     1801 attcctgatc aagatcggcc atatttaatt ttttctcaaa ctcagattca ttattattgc
     1861 taaaatagga aaaaggaatt tctttcctgc taattgatga cgtttttcta aaatagtcta
     1921 tcatcctctt ttttatcacc atttctgcaa atttgaggaa gctcctgctt ctactaatgt
     1981 caaacttttc aattgcttca ttaaatgcca tcagcccaat gctatattca tcactgcttt
     2041 ttaaatccac catttttgag gaataaaagc ctgaaacaat atttaatata aatggaatat
     2101 agtcttttat gaacttttct tttaaatact tgtccccgtc cttgattttt tcaacaattc
     2161 gggttgccgt ttcactggta taatctacaa tagcagaaat aaatagcata tctttcttcc
     2221 ctcattaata atattccgaa aaccaaaata ttgattttaa gtttagcaca aaaatattgt
     2281 ctgataattc cgtattaata ataattatac gacaaaatct ttctttttgg gctgcaaatt
     2341 acataattaa tatcggaata attataccgg aaaagaagct attttcccta atatataccg
     2401 tttaagcaat gtaaggtcgg tagaatttac ttctccgtct ccgttagtgt cggcattgga
     2461 aagattttct cccgaaagag acatcttttt aagcaaatac ctctttaata tggtaacatc
     2521 tgtcgaatca atctctccgt cgccattcac atcacctttt ctacccgcac cgccatcttc
     2581 atcaaatgta aaccagtcaa tatttacagc acccgaaaat ttaagatata agtcatgttt
     2641 ccccgttact ttgctaacgc tacagctttg ctcctcgtat gcattaaagc caccggttga
     2701 tgccaccggt aatgtgccta taagagttcc ggtaggactg tttaacctca gttcgatatt
     2761 cggagtaccg gtagtagcaa ccaatgcttt aaacacagtt gccccgtttc cgaagtcaat
     2821 acttttgtaa accaggtaat caccgttttc aatatatcct ataccgcttc cgccaccggc
     2881 agtaccaatt atttgcattg tggatgaatt aatgtcatta tattcctcgg cctctattct
     2941 tgaaaatgca cttctttcaa ccacaggtgt cggtgaaggt gttggtggcg ttgtaggcgt
     3001 cggtattgta tcggaatcaa atttccacca actgaagtta aacaaatagc cgcttccgcc
     3061 ggtaaacctg aagaacaaat catgaacacc agttactcca ctgattgaac aggattttgt
     3121 tgtccatttc tgccaacctc cggttccttc taccttacag gttccaacca gtgtccctgt
     3181 aggaccgtcc aacctcactt caatatttcc accctccgta ttgcttgcca ctctggcctc
     3241 aaaacttgtt gcaccgccgc tgccgaaatc aacgccttta atttgaatcc aatccccgtt
     3301 ttctataaat gcaacattac gtcctccctc actgcattct tcggtttcaa ttcccgattc
     3361 tttatgcatg gttacggcag tgttttcagc gtaaggatcc acatatttta gctgtttcaa
     3421 gccatctacc gttggaacta cctgcttgat ggtaccgtca gagttgtaaa acaactgatc
     3481 aatacaaaca tttctttgat atacagggtc ttctccccgc tgttttgcca cagtcctgtt
     3541 atggtagacg caataccaat tgcccttaaa ctctacaata gtatgatggt tattattatt
     3601 atagttgtcg ggtggctgcg gcaatacaac tcctttatat gtaaaacctg aggtcgggct
     3661 atcactcatc atgtattcaa tcttagaggc accttgagag aaatcactgg cataggtaaa
     3721 ataatacttt cccttatact tgtgcataaa tgatgcctcg aaaaacctcg gtgccgacat
     3781 cgacatagct gaacctaccg tgcttatcat atcactgccc agcttaatga ctctgatatt
     3841 attttctccg tttccaccaa agtacatata agcctgtccg tcatcatcaa taaaaacacc
     3901 ggggtcaaaa agccacatat taggtgcagg ctgtactccc gaagtatttc cggttatcaa
     3961 agccttcgga agaggatctt tgaagggtcc ggtagggctg tcactcactg ccactcctat
     4021 cccgtttcct ccgttaccgt aatacatata aaacttattg ttacgataga caattgacgg
     4081 agcccaggaa actccggccc attgggaatc tctttttgca ttaaatactt ctccatggtc
     4141 ggtccagttt ttcaaatcat ccgtggatat acaagtaata tccggtatat tgtaggttga
     4201 ctgaccgggt gtgacatccg aatcatgaga acagtaaaca taaagtctgc cgttataaac
     4261 aactgcagtt gggtctgccg tataacgctg agaaaaaatc ggatagtccg caaaaccttt
     4321 ttccggtaac agcaatgcca aaaagcacac aattaatgct atcaaccata ccctgcctgt
     4381 ttttttcacc ataataccac tcccttgaaa ttataaaatt ttattttatc ttcagatttt
     4441 tgttcatttt ttattgtcca gccatatttc ttctattcac attatatgta cgaaggatat
     4501 gcttatttta agttgggaat ttcgacacga ttttttaaga aaatcaagca actagaaaac
     4561 aggattaata tcctgttttc tttgctctca cagaattgta agtacgtttt ttgtatacgg
     4621 ttcctgaaat tcgtatcatc gagcataaaa ggatgacagt accccgacat cgtatgtatt
     4681 aatatctatg ttcatacact atttttcaac tgttaagctt gagttaccaa tatagttgat
     4741 acatttctac tcttgacaaa gaggaacttg tttaaacgaa tcctgttccc ccaaatatta
     4801 catctttata ctttaacatt gtgtaatggg tcatcctcag caatgcaatg aaatgaaata
     4861 aaaatcaaca actaccaaaa tattcagctc gattaacaat ccaaagctta tcttcaatat
     4921 aaataactgc caatggatag tcatgtataa taatcacatt actatcatca ctcggatagc
     4981 aaaaatcagt ccaatactta aggaaaactc cccaagtagt ttctaccgaa cagctttcac
     5041 tccagaaaaa tgttattata gcttccttag atattaatat gttatcctgt agaaaattaa
     5101 c
//
LOCUS       DAOOJC010000139         5047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_13188_length_5047_cov_3.491987, whole genome shotgun sequence.
ACCESSION   DAOOJC010000139 DAOOJC010000000
VERSION     DAOOJC010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13188_length_5047_cov_3.491987"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLA01_05105"
     CDS             <1..357
                     /locus_tag="PLA01_05105"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HPD00711.1"
                     /translation="PTMSQELITQLKSGLIDTPSYMFLVASLERALTFVIHIGFSVLV
                     MLGIRRGKGLLYLLLAILLHMVLDAAVVIMSSYGVNAFIIEGFVGICTIASIVFLVMT
                     GNDAKDKEGEIGFINE"
     gene            875..2665
                     /locus_tag="PLA01_05110"
     CDS             875..2665
                     /locus_tag="PLA01_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPD00712.1"
                     /translation="MYKKTKTKVFLALTLVSVISLMSFIYAANAITEDGDKYEFEDGV
                     HDGAQIYTDYIGKNGDGDVFDLSGSTCSFLGQKGTSTSVNVEVDNAGLYEIIICYVQP
                     YDKNKKVQYLNVNGINQGEVSFPYTLKWRELSAGIVKLEKGANNIELEAYWGYTYFDY
                     LIVKPADDSITDLKVPKTLVNPNATQEAKALMCYLVDIYGQHILSGQQELCGSHNYEG
                     SEAEFTYIKQKTGEMPAVRGFDFMNYRGNGLMWDDLCAERVIEWYNEKNGIPTVCWHW
                     FSPGDIGKKADSSFYTESTTFSISKALTPGTEENIALLNDIDFMAGKLKQVQDAGVPI
                     LFRPLHEAEGGWFWWGAEGPEPCVKLYRLLYDKFTNEYGLNNLIWVWTSYDYETSAAW
                     YPGDDVVDIVGYDKYNAKDGLPNGSAISSTFYNLVKLTGGKKLVTMSENDTIPRVSNL
                     INEKAGWLYFCPWYGWWLTGEQNNPVDWLVEMYQSEYCITLDELPDLKTYPTTGFGTV
                     PTSTPTPYPTPTPTETPKIKHGDINGDGEIDSTDLTILKRYILRKINELSVSQEVADL
                     NGDGDVNSTDITLLKRYLLRKIKNFPVDEN"
     gene            2728..>5047
                     /locus_tag="PLA01_05115"
     CDS             2728..>5047
                     /locus_tag="PLA01_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPD00713.1"
                     /translation="MKKGRFFSKRIKAMVTSFVLLAGMIVPFNTTLNAAELEFNYAKA
                     LQYSIFFYDANMCGADVDENNLYDWRGDCHVYDAELLLDSINTNMSDGFISRNMSVLD
                     PDGNGTVDVSGGFHDAGDHVKFGMPEAYSGSTLGWGYYEFREQYEATGQDDHIETILR
                     YFNDYFMRCTFLDKDGNVVAFCYQVGDGDVDHAYWNSPEIDEMFRRGWFATEELPSTD
                     CVTAAAASLAANYMNFKDEDPEYALKNLDYAKALFAFAEKNPKEVNADGPKGYYGSTK
                     WQDDYCWAAAWLYLATQDDHYLDELFKYYDYYAPSCWTHCWNDVWTGTACLLAEIDDM
                     YDKDSDEFENRYRQAANKSPYEEIGFWNEIKKLIENWMYGKTVTISPGGYAFLNKWGS
                     ARYNTATQFVALVYDKHHGDTPSEYGEWAKSQMNYLMGDNPLNRCYIVGYSDISVKYP
                     HHRAASGLSKCEDPDPHRYVLYGALVGGPDDSDQHIDVTSDWVYNEVTIDYNAAFVGA
                     CAGLYRYFGDPSMQVTPDFPPPTGGGNDDEPGGTGYWVEAFGVDIVQSDGPKVTEITL
                     YVRSNVKKPSKKTLVRYYFDATGMSSLEPNKIELRQLYDQTAAETDYAAELTGPRHYK
                     DNIYYVEIFWDGFVIANSNKKYQFALGTYAWGNSWDPSDDWSYQGLKIEESNYTGIPE
                     KADNICVYDDGVLVGGTEPDGTTPATPTPAVTPEPTPTSGILYGDLNGDEEINSTDLT
                     LLKRYLLRKIDKFSVPEEAADLNGDKSINSTDL"
BASE COUNT         1597 a          834 c         1154 g         1462 t
ORIGIN      
        1 ccgacaatgt ctcaggagct aataacacaa ttaaagagtg gattgataga cacaccgtcg
       61 tatatgtttt tggttgccag tcttgaaaga gcacttacat ttgttattca tattggtttt
      121 tcagtgttgg taatgttggg tatacgaaga ggaaagggtt tattgtattt gcttttggca
      181 attctgttgc atatggtgct tgatgcagct gtggttatta tgagttctta cggggtaaat
      241 gcattcatta ttgaaggatt tgtcggtata tgtacaattg catcgattgt atttttagta
      301 atgacaggta atgatgcaaa ggataaagaa ggggagatag gatttattaa tgagtaaata
      361 tttttaaaaa tacagtattt cacttcgtga aatcccacta aaaagactct ggggacattg
      421 atatgctccc ggagcttttt gatattttac ttcgtttttt cacattaaca atgtaattat
      481 tgtgcttgat tcttaaaata catataaaaa agatgaaatg aacagactaa aatacaattt
      541 gcatttcgaa tatatataat atatatgcaa taggacaaat caattgtaat gttaagaaaa
      601 atatagtaag ataaatatga gaggtaaagt agcataatga ataataaaac gctaaattac
      661 agtagttgca gtaatgctat tcttttataa aatcagttga cacggttaat caattcaaaa
      721 ctcgttgtgc actattcttc atttcgattt cggttacttt actattttaa tataatagtt
      781 tatcccatcg ccgcaaaatg cttgtacaac attatttaga aaagcggtta ttaagtataa
      841 tattatttta tatttttcat agaggaggag ttatatgtat aagaaaacta aaacaaaagt
      901 tttcttagca ctgacattgg tatctgttat atccctgatg tcatttatct acgcagcaaa
      961 tgccataacg gaagatggcg acaaatatga atttgaagac ggcgtccatg acggagccca
     1021 aatttatacg gactatatcg ggaaaaacgg cgatggcgat gtgtttgacc ttagcggttc
     1081 aacctgcagc tttttaggtc aaaaggggac aagcacttct gttaatgttg aggttgataa
     1141 tgccggactt tatgaaatta ttatttgcta tgtacagccg tatgacaaaa acaaaaaggt
     1201 tcagtatctc aatgtaaacg gtatcaatca gggagaggtg agttttccat acactcttaa
     1261 atggagagag ctttcagcgg gtattgttaa gcttgaaaag ggagccaata acattgaact
     1321 ggaagcctat tggggatata cttactttga ctatttgatc gtaaaacctg ccgatgatag
     1381 tataacagat cttaaagttc ctaaaacatt ggtaaatcct aatgctaccc aagaggcaaa
     1441 agctcttatg tgttatcttg tggacattta cgggcagcat atactctcag gccagcaaga
     1501 gctttgcgga tctcataact atgaaggttc tgaagcggag tttacataca taaaacagaa
     1561 aaccggggaa atgcctgcag taagaggttt tgactttatg aattacagag ggaacggttt
     1621 aatgtgggat gatttgtgtg ccgaacgggt tattgagtgg tataacgaaa aaaacggcat
     1681 acctacagtt tgctggcact ggttttcgcc tggagacatc ggaaagaagg cggacagcag
     1741 cttttatacc gaaagtacaa ccttcagtat ttcaaaggct ttgactccgg gaacagaaga
     1801 aaacattgcc ttgcttaatg atattgattt tatggcagga aagctaaagc aggttcagga
     1861 tgctggagtt ccgatattgt tcagacctct tcatgaagca gagggaggtt ggttctggtg
     1921 gggagccgaa ggtccggagc cctgtgtcaa actatacaga ctattgtatg acaaatttac
     1981 aaatgaatac ggtcttaaca accttatatg ggtatggact tcttacgatt atgagacttc
     2041 agcggcatgg tatcccggtg atgatgtggt ggacattgta ggttatgata aatacaatgc
     2101 caaggatgga ttgcctaacg gaagtgcaat ttcctctaca ttttataatc tggttaaact
     2161 tacaggcgga aagaaattgg tcacgatgag tgaaaatgat acaattcccc gggtttcaaa
     2221 cctcataaac gaaaaggcag ggtggcttta tttctgccca tggtatggat ggtggctgac
     2281 cggcgaacag aacaaccccg ttgactggtt ggttgaaatg tatcagagtg agtactgtat
     2341 aaccttggac gaattgccgg accttaaaac ttatccaaca accggttttg gaacggtacc
     2401 gacatcaaca ccaactcctt atccgacacc aaccccaacg gaaacaccga aaatcaagca
     2461 tggtgatata aatggtgacg gagagataga ttccacggat ttgacaatat taaaaagata
     2521 tattctcaga aagattaatg aattgagtgt ttcccaagaa gtggcagatt tgaacggcga
     2581 tggagatgta aattccacgg atatcacttt attgaaaagg tatttgctga gaaaaataaa
     2641 aaactttcct gttgacgaaa actgaaatta attaagttaa agcattttat atttaatata
     2701 aatttattta agaagggaag ggataagatg aaaaagggta gatttttttc aaagagaatt
     2761 aaagctatgg ttacaagctt tgttctcctt gcagggatga ttgttccttt caatactact
     2821 cttaatgctg ctgagctgga atttaactac gcaaaagcat tgcagtattc aatatttttc
     2881 tatgatgcaa acatgtgtgg agccgatgtt gatgaaaata acctgtatga ctggagaggc
     2941 gactgccatg tttatgatgc ggaacttttg cttgactcga taaacaccaa catgtcagac
     3001 ggttttatca gtcgaaacat gagcgttctt gatcctgatg gaaacggcac agtcgatgtt
     3061 tcgggcggat ttcatgatgc gggtgaccat gttaaattcg gtatgccgga agcctattcc
     3121 ggatcaacat tgggatgggg atattatgag tttagagaac agtacgaagc aacgggacag
     3181 gatgatcata ttgagactat attgagatat tttaacgatt attttatgag atgtacattt
     3241 ttagataagg acggtaatgt agtagccttt tgttatcagg tgggtgatgg ggacgttgac
     3301 catgcttatt ggaattcccc ggagattgat gaaatgttca gaaggggatg gtttgctact
     3361 gaagaactgc cctcaacaga ctgtgtaact gcggcagcgg cttctcttgc ggcaaactat
     3421 atgaatttta aggatgaaga tcctgaatac gcactaaaga atcttgacta tgctaaagcc
     3481 ctgtttgcat ttgcagaaaa aaatcctaaa gaagttaatg cggacggacc taagggctat
     3541 tacggttcta caaaatggca ggatgactat tgctgggctg cggcatggct ttatttggcg
     3601 acacaggatg accattattt agacgaatta ttcaaatatt atgattatta tgcaccttcc
     3661 tgctggacac attgctggaa tgatgtttgg acaggtacgg catgtctgct ggctgaaata
     3721 gacgatatgt atgataagga tagtgatgaa ttcgaaaaca ggtatagaca agcggcgaat
     3781 aagagccctt atgaagaaat aggattctgg aatgaaatca aaaaactgat tgaaaactgg
     3841 atgtacggta aaaccgtcac tatttcacct ggaggatatg cattcctcaa caaatggggt
     3901 tctgcaagat ataacacagc tacacagttt gtagctcttg tatatgacaa acatcacggt
     3961 gatacaccgt cagaatatgg tgagtgggca aaatcacaga tgaattacct gatgggcgac
     4021 aatccgctta accgttgcta catcgtagga tacagcgata tttccgttaa atatccacac
     4081 catagagcag catccggttt atcgaagtgt gaggatcccg atccccacag atatgtttta
     4141 tatggtgctt tggtaggtgg cccggatgat agtgaccaac atattgatgt aacatcggat
     4201 tgggtttaca atgaagttac aatagactat aatgctgcct ttgtcggagc atgtgctggt
     4261 ctttacaggt atttcgggga tccttcaatg caggttactc cggattttcc tcctccaacg
     4321 ggtggtggaa acgatgatga gccgggaggc accggatatt gggtggaagc ctttggcgtt
     4381 gatatagtac agtctgacgg accgaaggtt acagaaataa ctctatatgt acgttctaat
     4441 gttaagaagc cttccaaaaa aaccttagta agatattatt ttgatgctac cggaatgtca
     4501 tctttggagc ctaataaaat cgaattgagg caattatatg accagactgc ggcagaaacc
     4561 gactatgctg ctgagcttac aggtccccgc cactataaag ataatattta ctatgtcgag
     4621 atattctggg acggatttgt aattgcaaat tccaataaaa agtaccagtt tgcattggga
     4681 acatatgcat ggggaaatag ttgggatcct tcagatgact ggagctacca ggggttaaaa
     4741 attgaagaaa gcaattatac cggaattccc gagaaagcag ataacatttg tgtttatgat
     4801 gacggcgttc ttgttggagg tacagaaccg gatgggacaa cccctgcaac tccaacaccg
     4861 gcagtaacac cggagccgac tcccacatca ggtattctat acggtgattt gaatggggat
     4921 gaggagataa attccacgga tctgacactg ctgaaaaggt atttgcttag aaaaatcgat
     4981 aaattcagtg ttccggaaga agcagcagat ttaaacggag acaaaagtat taattctacg
     5041 gacttga
//
LOCUS       DAOOJC010000140         5009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_13318_length_5009_cov_4.584578, whole genome shotgun sequence.
ACCESSION   DAOOJC010000140 DAOOJC010000000
VERSION     DAOOJC010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13318_length_5009_cov_4.584578"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            508..1833
                     /locus_tag="PLA01_05120"
     CDS             508..1833
                     /locus_tag="PLA01_05120"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HPD00714.1"
                     /translation="MPNYKHLTLDDRSFIHSSLNAGHSFRQIAHHLDKDPSTISKEVR
                     HRRIHLSAGAAGRTPNKCIHKKTCTISGLCDQPKCKHNFCRYCKRCNAFCSEFQEEHC
                     PSLSKPPYVCNGCKLIYGCVLTKYFYRALSANQAYQTKLVDCRSGLSYTEDELQYMDE
                     VITPLVKKKQSIHHICVTQADNILCSERTIYKLIEQGVLTVRNIDLPRKVRYRLRRQS
                     PTFKVDKSCRIGRTYQDFQNYLEQHPDTPIVQMDSVEGCKGGKVLLTIFFTQSNVMLM
                     YLRNHNTSQSVIDVFNSLYETLGRETFMRLFPVILTDNGSEFSNPRAIEYDASGQMRT
                     KLFYCDPSSPHQKPEIERNHEFIRMVLPKGKSFDELTQNDVDLLVCHINSLIRKKLND
                     RSPITTFSFFHGDLILPKLGLQAIPPDEVTLTPALLATSKEVNMHEKDL"
     gene            complement(2092..2625)
                     /gene="mog"
                     /locus_tag="PLA01_05125"
     CDS             complement(2092..2625)
                     /gene="mog"
                     /locus_tag="PLA01_05125"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HPD00715.1"
                     /translation="MEGFSVGIITVSDKGSRGERTDESGPAIEEIMKQIGGRIEKYVI
                     VPDEKRDIKEAIIDMSDMLGLNLILTTGGTGFSKRDVTPEATLEVVERYVPGIPEAMR
                     AKSLQVTPKAMLSRAQAGIRKQSLIINLPGSPKGVRENLEAIIPALKHGIEILTGQAS
                     ECGGKCLNEKVSSSLKD"
     gene            complement(2644..3189)
                     /gene="moaC"
                     /locus_tag="PLA01_05130"
     CDS             complement(2644..3189)
                     /gene="moaC"
                     /locus_tag="PLA01_05130"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HPD00716.1"
                     /translation="MNPKNKKEAQGVGEFTHINAKGYAKMVDVSEKEESVREAIARGS
                     VYMEKDTLKAIAEGRIRKGDVLSVAQVGGIMGAKSTPDIIPMCHNVSIYGADIEFKAD
                     YENSKIDIEAVVKTVGVTGVEMEALTAVSVAALTIYDMCKAIDKLMVISDIHLVHKRG
                     GKSGEFNFYDKLKEDISNTKC"
     gene            3409..>5009
                     /locus_tag="PLA01_05135"
     CDS             3409..>5009
                     /locus_tag="PLA01_05135"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPD00717.1"
                     /translation="MLKISSKSIMFKVLVMILAICTLESLLFTIMSERLTGSILENMT
                     IENSKKNAELYSEFIGSWLGERMREINIFANSPVIKTMDWNSIEPYLRQEVKEKLDVY
                     DHFMVAESNGNYSTTLRRNVGNVSDREYFKHVMEGKTVVSNPVISRSTGKPIAIIASP
                     ILDDNGKIKGVMAGAVNLMKLSKIIENLKYNYPNSYSYIVDKEGLIIAHPREELILQQ
                     NITKESDVITKNIAQASAEILTKDQGSNTYSFEGIVSMNYYHIIPNTDSWRLVIKIPV
                     NYWHTPMRYTSSRLILIGLAGLIIASIVGFFIAKSMSTPIIKLKEVFTRATAGDLTVR
                     SEISTNDEIGDAARSFNKMMDTISKLTYYDSLTLLPNKLHFDAILCREIKKSARNNTM
                     LALMIFDIDRFESINNALGHSVGDKLLKSLAEKISCFEDNNHVVARIGEDMFAVLFKN
                     FSDKEDVIKLSDEIRQIIRQPWVIDEHRFYITACVGIALYPKDGESGDSLFKNAFSAM
                     QRAKVYGRDNYNFYEPAINELLLEQ"
BASE COUNT         1484 a         1120 c          917 g         1488 t
ORIGIN      
        1 aattacaata ccctaaaaac cacttatcaa atattttttt ctcatttttt acagcgtaaa
       61 ttgcaaaagt aagttctacc cttacttagc aaactataga atttaatcat tcaaatccac
      121 atgtagaatt taatcgtgca attatataac acctcttttt tcctccagag cccgcccgag
      181 cttcagagaa aaagtttttt aagtgcggtt tacaatgagt gggtcccgta tgatagcctt
      241 ctggctagcc agctttctat gcccctcagc catacttcat ccaatacttc gggggcatgg
      301 ggagatggcc cgctggcaat ctgtcatatg ggggaggctc gttgtcaact ggattcttga
      361 aaaagttttt cacctcatta tttgccattc taaagtctta tattatctaa atttacaggg
      421 cttacgcttt catttaatgg tataaccttc tataattatg atggatgatg ttactcagca
      481 agccggtgta tgaaaggagc gctagctatg ccaaattata aacacttaac cctggatgac
      541 cgcagtttta ttcattccag tctgaatgcc ggtcattcct tccgtcagat tgcacatcat
      601 cttgacaagg atccttccac tatctccaag gaagtccgac atcgccgtat acacttgtca
      661 gcaggtgctg ccgggcgcac acctaacaag tgtattcata agaaaacatg caccataagc
      721 ggtttatgtg atcagcctaa atgtaaacat aacttctgcc gttactgtaa aagatgcaac
      781 gcattttgtt cagaattcca ggaagagcac tgtccctctc tttcgaagcc cccttatgtc
      841 tgtaacggtt gtaagctaat ctatgggtgc gttctgacca aatattttta tcgggctttg
      901 tctgccaacc aagcgtatca aaccaaactg gttgactgcc ggtcaggcct ttcatatacc
      961 gaagatgagc ttcagtatat ggacgaagta attacgccac tggtcaaaaa gaaacaatca
     1021 attcatcata tctgtgtgac ccaggcagat aacatcctat gcagtgaacg tactatctat
     1081 aagttaatcg aacagggtgt tttgactgtc cgaaatattg acctgccccg caaggtacgc
     1141 tatcgactca gacgccagag cccaacattc aaagtggata agagctgtcg tataggtcgt
     1201 acatatcagg acttccaaaa ctatcttgaa caacatcctg atactcccat tgttcaaatg
     1261 gattctgttg aaggatgtaa gggtggaaaa gtactactaa caatcttctt tactcaatca
     1321 aacgttatgc tgatgtacct tagaaatcac aacacatctc aatcggttat cgatgttttt
     1381 aactctcttt atgaaacgct tggcagggag acattcatgc ggttgttccc tgtcatttta
     1441 actgataacg gttcggaatt ctccaatcca cgtgctatcg aatatgatgc aagcgggcaa
     1501 atgcgtacaa agttgttcta ttgtgaccct tcctcaccac atcaaaagcc tgagattgag
     1561 cgtaaccatg aatttattcg catggttctg cctaaaggta agtctttcga tgaattgacc
     1621 caaaatgatg tggatctgtt agtctgtcat atcaattcct tgataagaaa aaagctgaac
     1681 gataggtccc ccataaccac attcagcttc ttccacggag atttgattct ccccaaatta
     1741 gggcttcagg ctatcccacc ggatgaggta acacttactc cggcattgct ggctacctca
     1801 aaggaggtga acatgcatga aaaagaccta tgacaatatc cgactgactc tcgatccaaa
     1861 gagctcaaac ttagatcttt aaaccaccct aatgcctgct tgaggaacat catccatttc
     1921 aattagtctt tttccatggg tagaacttag ctatgcgaaa aactgcactt ttttctacct
     1981 tctgtttatc atacacattt tttgcccaaa agccttaatt ctacttcaat tctatcacca
     2041 aatgacggtt aaaaacaggt cttacctata tacttatatt ttttgtggaa tttaatcctt
     2101 caaactagaa cttacttttt catttaagca ttttccgccg cactccgatg cctggcctgt
     2161 caatatttct atcccatgct tcaatgccgg aattatagct tccagattct ctctcacacc
     2221 ctttgggctg ccgggaagat ttataataag ggactgtttc ctgatgccag cctgtgccct
     2281 ggagagcatt gccttcggtg taacctgaag gctcttcgcc ctcattgcct cgggtatccc
     2341 cggtacatac ctttcaacta cttcaagagt tgcttcagga gttacgtctc tttttgaaaa
     2401 gcctgtacct ccggtagtca gtattaaatt caatccgagc atatcggaca tatctatgat
     2461 agcttccttt atatcccgct tttcatcagg aacaatcaca tatttttcta tgcggccgcc
     2521 tatctgcttc attatctcct cgatagcagg tcctgattca tctgttcttt cgccccttga
     2581 ccccttatcg cttactgtta tgattcctac actgaatccc tccattggat cctacctccc
     2641 gcactagcac ttcgtattac tgatatcttc tttaagcttg tcgtagaaat tgaattcccc
     2701 cgacttgccg cccctcttat gtacaagatg tatatcactt atcaccatta atttatcaat
     2761 ggccttgcac atatcatata tagtcagcgc cgcaacggat actgccgtca aggcctccat
     2821 ttcaactccc gtcactccga cagtcttcac aaccgcctca atatcaattt tggaattttc
     2881 ataatcagcc ttaaattcta tatctgcacc atatatgctg acattatggc acataggtat
     2941 tatatccggc gtagacttgg ctcccattat accgcctacc tgcgctacgg ataagacatc
     3001 tccttttctg atccttccct ctgcaatggc cttcagggta tctttttcca tataaactga
     3061 acctcttgca attgcctctc ttacactctc ttccttttca gaaacatcca ccattttggc
     3121 atagccctta gcatttatat gggtaaactc cccaacaccc tgggcttcct ttttattttt
     3181 ggggttcata caatcacctc gttatctcat attttagcac aaactcaaga aattaataag
     3241 gccaatgcta taatttatca aagaacctct atgaccgata ttaaaattaa aaagtatgtt
     3301 ttttaattaa cattatttat actatattaa tgtgccaatt tttgatataa tttggcatgg
     3361 atggtatttt accgtcattt tttcaacttt atcaaggagg acagcgggat gttgaagata
     3421 agcagcaaaa gtataatgtt caaagtgctg gtgatgatat tagccatatg tacgttggag
     3481 tctttacttt ttactataat gtcggaaaga ctcaccggca gcattttgga aaacatgaca
     3541 atcgaaaaca gtaaaaagaa tgcagagctg tactccgaat tcataggcag ctggctgggg
     3601 gaacgcatgc gggaaattaa tatatttgca aattctcccg ttataaaaac aatggattgg
     3661 aatagcatag aaccctacct gaggcaggaa gtcaaagaga aacttgatgt atatgatcat
     3721 tttatggttg cggagtcaaa cgggaattat agtacaactc ttagaagaaa tgttggaaat
     3781 gtaagtgacc gtgagtattt caagcatgtc atggaaggca aaactgtagt gtccaatccg
     3841 gttatctcca gatctaccgg aaagccgata gcaattattg catcacctat actggacgat
     3901 aacggaaaaa ttaaaggggt tatggcagga gcagtaaacc tcatgaagct ttcaaaaatt
     3961 attgaaaatc tcaaatataa ctatcccaac tcatactcat acattgtaga caaagaaggc
     4021 ctaatcattg cccatcccag ggaagaatta attctccagc aaaatataac aaaggaatca
     4081 gatgtcataa ctaaaaacat agctcaagct tcggcagaga tactcacaaa ggaccagggc
     4141 tccaatacat attcctttga gggtattgtc agcatgaatt attatcatat aatacccaat
     4201 accgacagtt ggaggctggt tatcaaaata ccggtaaact actggcatac ccctatgaga
     4261 tatacttcat cgcgtcttat actaattggg cttgcaggcc tgataatagc ttcaatagtc
     4321 ggattcttca ttgcaaaaag tatgtcaact ccaatcataa agcttaaaga agtatttaca
     4381 agggcaacag ccggagacct tacagtaagg tcggaaatca gtaccaatga tgaaatcgga
     4441 gatgccgcaa gaagctttaa taaaatgatg gataccataa gcaagctgac ctactacgat
     4501 tcacttaccc tgctccccaa taagcttcat tttgatgcaa tactgtgcag ggagattaag
     4561 aaatccgccc gtaataatac catgcttgct ttaatgatat ttgacataga caggtttgaa
     4621 agcattaaca atgctttggg ccattcggtg ggtgacaagc ttctgaaaag cctggcagaa
     4681 aaaatcagct gctttgaaga caataaccat gtagttgccc gcatcggaga ggatatgttc
     4741 gcagtattgt ttaagaattt tagcgataag gaagatgtta taaagctgtc ggatgaaatt
     4801 cggcaaatta tcagacagcc ctgggtaata gatgaacaca gattttatat tactgcctgt
     4861 gtagggattg ccttatatcc taaagacggg gaaagcggcg acagcctgtt taagaatgca
     4921 ttttcagcaa tgcagagagc taaggtatac ggccgggata attataattt ctacgaacct
     4981 gcaataaacg aactgcttct tgaacaaat
//
LOCUS       DAOOJC010000141         5001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_13341_length_5001_cov_3.429438, whole genome shotgun sequence.
ACCESSION   DAOOJC010000141 DAOOJC010000000
VERSION     DAOOJC010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13341_length_5001_cov_3.429438"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..2591)
                     /locus_tag="PLA01_05140"
     CDS             complement(63..2591)
                     /locus_tag="PLA01_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPD00718.1"
                     /translation="MSKRFIGKFKAIAFTAAFAFTSIFASPVSTEAVGSIPYKWDNVV
                     IGGGGGFVPGIIFSETEKDLIYARTDIGGAYRWDPSTGTWIPLLDHFQMDEYSYYGVE
                     SLAADPVDPNRVYIAAGMYTNDWLPNMGAILRSNDRGETWEKTQLPFKFGGNMPGRSM
                     GERLAVDPNDNSILYFGARCGKGLWRSTDYGVTWSEVKNFPNPGDYIYDPNNDYTKDI
                     IGVVWVAFDKSSSTRGNATKNIYVGVADKDESIYRSTDGGATWEAVPGQPTGLLPHHG
                     VVASNGMLIITYGDTCGPYDGGGKGQVWKYNTKTGEWTDISPLPYSSSDNRFCFAGLA
                     GDAQNHDVMLVSTMNAWWPDEFIFRTTDGGKTWKNIWEWGMYPERILHYEQDISKAPW
                     LDWGTEKTLPEINPKLGWMIGDIEIDPFNSDRMMYGTGATLYGTDNLTDWDKGGKVKI
                     EVKGMGIEECAVLGLISPPEGAPLVSAVGDIVGFYHEDLKVGPEKFHVSAYSTGTGID
                     YAELSPNFMALVSENESNAVKRISFSYDGGKNWFQASSEPGNSVGGGKVAVSADAKAV
                     IWTPKDSAPALTTDNGNSWTVCRGLGNGAKVASDRADGKKFYAFYNGKFYVSTDGGEN
                     FTATTASDLPELVNDIKAVPGKEGHVWMAARDGGLWKSADGGAVFTKLSNVSAANVVG
                     FGKAAPGQDYMAVYITGEIDNVLGFFRSDDAGKTWMRINDDEHGYGAVDTAITGDPRV
                     YGRVYVATNGRGIVYGEPDSDIPVPTPTHLPSIKVGDVNGDGSIDSTDLTLLKRYVLR
                     KIDDFEVEDDLWAADLDGDGDINSTDVTLMKRFILRKIKEFPKK"
     gene            complement(3006..3884)
                     /locus_tag="PLA01_05145"
     CDS             complement(3006..3884)
                     /locus_tag="PLA01_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPD00719.1"
                     /translation="MMEMNNGFECNNKDCASGGCSQESCPQKGSSSIKQDFIAKTNEL
                     NHINKVIGIVSGKGGVGKSLVTSTLAVLMKRKGYRVGVLDADITGPSIPKMFGVSKKA
                     EGSELGIHPQRTHNDIKVMSVNLMLKKEDSPVIWRGPIISGVVKQFWTDVIWGDIDYL
                     FIDMPPGTGDVPLTVFQSIPLDGVVIVTSPQDLVSLIVKKAYNMAKSMNKTVLGIVEN
                     MSWIKCPDCGREIELFGKSKIDDVARDLGVPVLGRVPIDPSVAELCDKGEIEKVNHDY
                     LNKAVECIENIGGSKG"
     gene            complement(4006..>5001)
                     /locus_tag="PLA01_05150"
     CDS             complement(4006..>5001)
                     /locus_tag="PLA01_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD00720.1"
                     /translation="EAIIDRQNRSKTMRKTPKRMGNSEARLHKQDAKQKQQKIDKAVN
                     SLKTRLEKLEVKERPRSIPRIKLDFSLTDPPKNKVVISSDGLTFGYGSKKIFDNVKFK
                     VYNGSKTALWGENGAGKTTLLNLIGCFSDESINVVPKAKLGYFRQGFENLECDKTVLE
                     NVMKDSIQSQSVARTILARLLISGEDVHKRVGVLSGGEKIKVSFAKLFVSDANVLLMD
                     EPTNYLDMQSIEALEDVLLEYEGTVLFVSHDRGFVNKVADRLLIVENREIIEFEGTPK
                     EYFDGRKKPVIMANSEAERIALQMKITEVVARLSEPGEDKEMLEEEYRRLIQQLK"
BASE COUNT         1307 a         1329 c          863 g         1502 t
ORIGIN      
        1 accaacttac agtatcaaat aattagctca gactgtacat tgctctgcat taattccaat
       61 agctattttt taggaaactc tttaattttt ctaagtataa atcttttcat taatgttaca
      121 tccgttgaat ttatgtcgcc atcgccatct aaatccgccg cccacaagtc atcttcgacc
      181 tcaaaatcat caatctttct taaaacatac ctcttcaaaa gtgtaaggtc tgttgagtca
      241 atgcttccgt ctccgttaac atcaccgacc ttgatactgg gtaaatgtgt aggggtcggt
      301 accggaatat ccgaatcagg ctcaccgtaa acaatacccc ttccgttggt agcaacataa
      361 acccggccat aaaccctggg atctcctgtt attgccgtat caacagcacc ataaccatgt
      421 tcgtcatcat taatacgcat ccacgtctta ccggcatcgt cggagcggaa gaatcccaaa
      481 acattgtcta tttcgccggt tatataaaca gccatgtaat cctgtcccgg tgcagctttt
      541 ccaaagccca ccacatttgc tgcagataca ttcgaaagct ttgtaaatac agcaccaccg
      601 tcagcagatt tccataatcc tccgtcccgg gcagccatcc aaacatgtcc ctcttttccc
      661 ggaactgcct tgatatcatt aaccaattca ggcaaatccg aagcagttgt tgcagtaaag
      721 ttttctcctc catcggtact tacataaaac tttccattat aaaaggcata aaatttcttt
      781 ccgtctgcac ggtcagatgc aacctttgcc ccgttaccaa ggcctctgca gacagtccag
      841 gaattgccgt tgtcggttgt tagtgccgga gcggaatcct tcggtgtcca gataactgct
      901 ttggcatcgg cagaaacagc aacctttccg ccgcccactg aatttcccgg ctcactggat
      961 gcctggaacc agttctttcc cccatcatag gagaaggaaa tacgctttac tgcatttgat
     1021 tcgttttccg aaaccaaagc cataaagttc ggtgaaagct cagcatagtc aatgcctgtt
     1081 cctgttgagt aagctgatac atgaaacttt tccgggccaa ctttcaaatc ctcatggtaa
     1141 aatccaacaa tatctcctac tgcactaaca agaggagctc cctccggcgg acttatcagg
     1201 cctaaaaccg cacattcctc aattcccatg ccttttactt caatttttac cttccctccc
     1261 ttgtcccagt cggtaaggtt gtcggtaccg tataatgtag caccagtacc gtacatcatg
     1321 cggtccgaat tgaacgggtc aatctctata tcgcctatca tccagcccaa tttgggattt
     1381 atttccggaa gtgttttctc tgtaccccaa tccaaccatg gtgctttgga aatatcctgt
     1441 tcgtaatgca gtatacgttc gggatacatt ccccattccc atatattttt ccatgtcttt
     1501 cctccatcgg tagtacggaa aataaactca tccggccacc aagcattcat ggtagacaca
     1561 agcataacat catgattctg tgcatcccct gccaaccctg caaagcaaaa acgattatct
     1621 gagctggaat acggaagggg actaatatcg gtccattcac ctgtcttggt attatacttc
     1681 catacctgcc cctttccgcc gccgtcataa ggaccgcagg tatcgccata ggttatgatc
     1741 agcatcccgt ttgatgccac aactccatga tgaggaagca atcctgtggg ttgtccggga
     1801 accgcttccc atgttgcacc tccgtcggta ctgcggtaaa tgctctcgtc cttgtcggcc
     1861 acccctacat atatgttttt ggtagcattg ccgcgggtac tgctgctttt atcaaaggcc
     1921 acccaaacca ctccaattat atctttggtg taatcattgt tcggatcata aatataatct
     1981 ccgggattag ggaaattctt tacctcagac catgttacac cataatcggt acttctccac
     2041 aaaccttttc cgcagcgggc accaaaatac agtatgctgt tgtcattagg gtcaaccgcc
     2101 aaacgttcgc ccatggaacg tccgggcata tttccgccaa acttgaaggg cagctgtgtt
     2161 ttttcccacg tttcgcccct gtcattggaa cgtaatatgg cacccatgtt cggaagccaa
     2221 tcgttagtat acattccggc tgctatataa acccgattcg gatctaccgg gtcggcagcc
     2281 aggctttcca ctccatagta gctgtactca tccatttgaa aatggtcaag taatggaatc
     2341 catgtcccag tagaagggtc ccaacggtaa gctcccccga tatctgtacg ggcgtaaatc
     2401 aaatcttttt ccgtttcgct gaatattatt cccggaacaa atcctccgcc tccgccaatc
     2461 accacattgt cccatttata cggaatactg ccgacggcct cggtagaaac aggggaagca
     2521 aatattgatg tgaatgcaaa tgctgctgta aacgcaatag ctttaaactt gccgataaac
     2581 cttttactca ttagccgatc ctccctataa aaaatataga aattttcctt tcttttcttt
     2641 ctctaacacc cttattcaaa gaaaatttta cgactgtaga aatttcttcc ctttgcggtg
     2701 tttttagcaa ggcggaagat atgccggtcg cctgtaagtc caatctgtat ctgccactta
     2761 taagagcaag ggacattttc gcctcgtaat aagaattcgc tttaaatggg ttctttaatg
     2821 tatcctcgtt tttgaacatt ctcttttgtc aaatcccgcg agcaaaatct ccgggagcat
     2881 caagcttcca tcgttttttg tcatttcact tcgtgaaata ccacgaaaaa accgttgatc
     2941 caggtattat cggaccaacg gttgttaaca gtcaatacat tgtcattata aaccactata
     3001 ggattttaac ctttactgcc tccaatattc tcaatacact ccacagcttt attgaggtaa
     3061 tcatgattca ccttttcaat ctctcctttg tcacaaagct ctgcaaccga aggatcaata
     3121 ggtactcttc ccagcaccgg cactcccaga tcccgggcaa catcatctat cttgcttttc
     3181 ccgaataatt cgatttctct tccgcaatcg ggacatttaa tccaactcat attttcaaca
     3241 atacccaata ctgtcttatt catggatttt gccatattat acgctttttt cacaataagt
     3301 gaaaccagat cctggggcga agtcacaata accacaccat ccaagggtat ggattgaaaa
     3361 acagtcaaag gcacatctcc ggtccccgga ggcatgtcaa taaacaggta gtcaatatcc
     3421 ccccatatca catcggtcca gaactgtttc acaacaccgg agattatggg tcctctccat
     3481 ataaccggag agtcttcctt tttaagcatc aggttgactg acattacctt aatatcatta
     3541 tgtgtacgtt gaggatggat tcccaactcg cttccttcgg cctttttgct gactccgaac
     3601 attttgggta tggatggacc ggtaatatcc gcatcaagca caccaaccct gtaacctttt
     3661 cgtttcatca atacggccaa cgttgacgtt acaagggatt tgccaactcc accctttccg
     3721 cttactattc ctatcacctt attgatatgg ttaagttcat tggtctttgc aataaaatcc
     3781 tgtttaattg aactgcttcc tttttgagga cagctttctt gggaacaccc gccggaagca
     3841 cagtctttgt tattgcactc gaaaccgtta ttcatttcca tcatctccta tgtgttgttt
     3901 ttttatgtcc taaaatagta tataccaact attgtcctgt tataaaccct tacaagactg
     3961 aacttcttcg cccctttaat tatgaatatg caataaaaca gatttctact tcaattgctg
     4021 tatcaatctc cggtactcct cctcaagcat ttccttgtct tcaccgggtt ccgaaagcct
     4081 tgccactacc tctgtaattt tcatttgcaa agcaatcctt tctgcctcac tattagccat
     4141 gataaccggc ttcttcctgc catcaaaata ctcctttgga gttccttcaa attcaattat
     4201 ttcccggttt tcaacaatga gcagcctgtc ggcaacttta ttaacaaatc ccctgtcatg
     4261 agaaacaaac aaaacagttc cctcatactc taaaagaaca tcctccaagg cttctatgga
     4321 ctgcatatca agataatttg tgggttcatc catcagaaga acattggcat cggacacaaa
     4381 aagctttgca aaagatactt ttatcttttc acctccgctt aacacaccaa ccctcttgtg
     4441 tacgtcctca cccgaaataa gaagccttgc caatattgtt cgtgccactg actgggactg
     4501 tatactgtcc ttcattacat tctccaatac ggttttgtcg cattccaggt tttcaaaacc
     4561 ctgccggaaa taacccagtt ttgctttagg aacaacatta atactctcat ccgaaaaaca
     4621 gccaatcagg ttaagcagtg tggtcttccc tgccccgttt tccccccaca aagcggtttt
     4681 cgatccgtta taaaccttga acttcacgtt gtcaaatatc ttcttgctgc catagccaaa
     4741 ggtcaaacca tcggaagaaa ttacaacttt gttcttcggc ggatctgtca aagagaaatc
     4801 cagcttaatc cttggaatcg atcttggcct ttctttaacc tccagctttt cgagccgggt
     4861 cttcagacta ttaactgctt tgtctatctt ttgctgtttc tgtttcgcat cctgcttatg
     4921 aagccttgcc tcggaatttc ccatccgttt tggggtcttc ctcatggttt tggacctgtt
     4981 ctgcctgtca attattgctt c
//
LOCUS       DAOOJC010000142         4989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_13380_length_4989_cov_3.455817, whole genome shotgun sequence.
ACCESSION   DAOOJC010000142 DAOOJC010000000
VERSION     DAOOJC010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13380_length_4989_cov_3.455817"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PLA01_05155"
     CDS             <1..453
                     /locus_tag="PLA01_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HPD00721.1"
                     /translation="NYITRAETIKILNKVIPYLYNNKGSYAAEEINGNVLINSEGVVL
                     ANTVVNGDLFLAPGIKDGDVTLDGVNVKGVVYVNGGGSDSVHLINTKLNRIIVNKKGD
                     EEVRIVTSGSSSIGSIVVKSGVKLEEKDLDADGFNEVIVDSGLSAGRK"
     gene            477..2894
                     /locus_tag="PLA01_05160"
     CDS             477..2894
                     /locus_tag="PLA01_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HPD00722.1"
                     /translation="MEVQAEEALLETKEAKMKLKVLAQKVKINGKEIEKSSNNYIVAG
                     ELASIEIDEPKPSSVPGADNTPEPSGVPAENSNSGQKNTPKKSSSPKPTVTPVPTISP
                     SAPKEEWHLVWSDEFDGSEINMDNWSYDDPTNGRWNSEIQSYTQNNAFIEDGSLIIEA
                     RKEDITEPSGGTYNYSSAKMITKGKQSWKYGKFEIRAKMPTGQGIWPAIWMMPEDEPF
                     YGIWPKCGEIDIMELLGHIPNKLHGTLHFGEPHKESQGTYFLPEGQSFGDDYHVYAIE
                     WEPGEIRWYIDGELYHIENDWYSRDQYLADDYTYPAPFDQNFFLIMNISVGGSWPGYP
                     DETTVFPQQMAVDYVRVYQKDEYPHREKPVKEEGTARKPLEDGNYIYNGGFDVDDPEA
                     TGVEGVSNTSYWTFLQGPGGVSAVNVDEGVMHVQVEKGGTTDYSVQLLQAPVHLEKGA
                     KYKASFDMKAQNTREVKLKIGGDGDRGWKDYAAIAPFEVSTEMTAYEFEFTMKDDTDV
                     KARFEFNMGLDDNDIWIDNVKLIKTEDAPEIDPSTVVRPPLLSGNYIYNGTFDQGENR
                     MGFWNFAVDGSAKAAYYIGSEVNERRFETRIQKGGKSKDAIRLVQPGINIEKGKSYKV
                     SFEACAEAQRSIDVEIASNLNGSSIFSTTFEIGKENKIYEFEFTMNEESDKNGELRFN
                     LGGNNIDVYIDNVVMKKTSSDEVEGNLLLNGVFNSLLSWKYEAYDPGSALFDNPDEQF
                     RADISSVGTAGWNVQLYQDNVPLEEGQTYEVSFSAKSTVDRKVLIQLQEVDVWTQYFL
                     SGSSNYR"
     gene            2848..3531
                     /locus_tag="PLA01_05165"
     CDS             2848..3531
                     /locus_tag="PLA01_05165"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HPD00723.1"
                     /translation="MSGHNTSYQEAQITGDLQTYTYEFTMDKPTDSASRFSIALGSPD
                     DGKTTYDPHEVIIDNVVVRKVVPVISLIQNGTFENDKEHWLEYWGGSWEDDPAGAGNG
                     TATGSCIVTAGELEVNITKVAVKEYTPQIKQENLLLEKDATYILSFKARSLDARTIKA
                     DILDSVYNWYGGSTFDLTTEDEVYTFTFTPTKDISDGVLTINFGTIPGKTSVATTIYL
                     DDITIIKQK"
     gene            3861..4424
                     /gene="ahpC"
                     /locus_tag="PLA01_05170"
                     /pseudo
     CDS             3861..4424
                     /gene="ahpC"
                     /locus_tag="PLA01_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
     gene            4437..>4989
                     /locus_tag="PLA01_05175"
     CDS             4437..>4989
                     /locus_tag="PLA01_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPD00724.1"
                     /translation="MLLDVEIKQQLEQYLALLENDIVIKVSAGNDKVSTDMVVLIDEI
                     ARLSSKIHVEKAELDRTPSFAISRLNEDTGIVFAGIPLGHEFTSLILALLQVGGRAPK
                     VDETNIQRIEQLKGEYRFETYISLSCHNCPDVVQALNIMSVVNPSITHTMIDGAVFKE
                     EAESKDVMAVPAIYLNGKFFDNGR"
BASE COUNT         1705 a          815 c         1136 g         1333 t
ORIGIN      
        1 aactacataa cccgtgcaga gacaattaaa attttaaata aagtaattcc ttatttatat
       61 aataacaaag gcagctatgc agctgaagaa ataaacggaa acgtactgat taattctgaa
      121 ggggttgttt tggcaaacac agtagtaaat ggtgatttat ttcttgcccc gggaattaaa
      181 gatggcgatg taacccttga cggtgtaaat gtgaagggag tagtttatgt aaatggtgga
      241 ggaagcgata gtgtacattt aataaatacc aaattaaaca ggattattgt taataaaaag
      301 ggtgatgagg aagttagaat tgtaacttca ggaagtagtt ctattggaag tattgttgtt
      361 aaatccggtg taaaacttga agaaaaggat ttggacgccg atggatttaa tgaagttatt
      421 gttgactcag ggctttcagc cggcagaaaa tagtatttgt cggcgatttt gaacaggtgg
      481 aggttcaagc agaagaggcc ttgctggaaa ctaaagaagc aaaaatgaaa ttaaaagtat
      541 tagcccaaaa ggttaaaata aatgggaaag aaattgaaaa atcatcaaat aattatattg
      601 tagccgggga attagcatca attgaaatag atgagccgaa accttccagc gtaccgggtg
      661 cagataatac cccggaacct tccggtgtac cggctgaaaa tagtaactcg ggacaaaaaa
      721 atacaccgaa aaaatcaagc agcccaaaac caaccgttac tcctgtacct accataagtc
      781 ccagtgctcc gaaagaagag tggcatctgg tttggagtga cgagtttgac ggctcggaaa
      841 taaacatgga taattggagc tatgatgatc caaccaacgg aagatggaat agtgaaatac
      901 aatcctacac acaaaataat gcatttatcg aagacggttc actgattatt gaagccagga
      961 aagaagatat tacggagccc agcggtggaa cctataatta ttcatcggca aaaatgatta
     1021 ccaaaggcaa acaatcatgg aaatacggta agtttgaaat aagggcaaaa atgcctaccg
     1081 gacaaggaat ttggccggca atttggatga tgccggaaga tgaacccttc tacggaattt
     1141 ggcccaaatg cggtgagata gatattatgg aacttctggg gcacataccc aacaagcttc
     1201 atggaacact tcattttgga gaaccccaca aggaatccca gggaacatat ttcttgccgg
     1261 aaggacagag cttcggtgat gactatcatg tttatgcaat tgaatgggaa ccgggtgaaa
     1321 ttcgctggta tatagacggt gagttatacc acattgaaaa tgactggtac tcaagagacc
     1381 agtatctcgc tgatgattac acttatcctg caccttttga tcagaatttc tttttgatta
     1441 tgaacatatc cgtaggagga agttggccgg gatatcctga cgaaacaacg gttttcccgc
     1501 aacaaatggc tgtggattat gtgagagtgt atcaaaagga tgaatatcct catagagaaa
     1561 agcctgtcaa agaagaaggg acggcaagga aacctcttga ggacggcaat tatatctata
     1621 acggcggttt tgatgtagat gatcctgagg caaccggtgt tgaaggagtt tccaatacat
     1681 cttactggac attcttacag ggacctggcg gagtttcagc cgtaaatgta gatgaaggtg
     1741 taatgcatgt acaggtcgaa aaaggaggaa caaccgacta cagtgtacaa ttgcttcaag
     1801 caccggttca tcttgaaaaa ggtgcaaaat ataaagcatc tttcgatatg aaagcccaaa
     1861 acacaagaga ggtaaaactt aaaataggag gagacggcga caggggctgg aaagattatg
     1921 cagctattgc accctttgag gtttccacag agatgactgc ctatgagttt gagtttacca
     1981 tgaaagacga tacggatgtt aaggcacggt ttgagtttaa tatgggtctg gacgacaatg
     2041 atatctggat tgacaatgtc aagctgatta agacagaaga cgcaccggag atagatcctt
     2101 caacagtagt aagacctccc cttctttcag gaaattatat atacaacggc accttcgatc
     2161 aaggcgaaaa cagaatggga ttctggaact ttgctgtaga tggcagtgca aaggctgcat
     2221 actatattgg aagtgaagtt aatgagcgta gatttgaaac aagaatacaa aaaggcggaa
     2281 aatccaaaga tgccattaga ttggtacagc cgggaattaa tattgaaaag ggcaaatcct
     2341 acaaggtaag tttcgaagca tgtgccgaag cacaaagaag cattgatgtg gaaattgcaa
     2401 gcaatctaaa tggtagcagc attttttcaa ccacttttga aatcggcaag gaaaataaga
     2461 tatacgaatt tgaatttacc atgaatgaag aatcggataa gaacggggaa ctcaggttca
     2521 atttgggtgg aaataacatt gatgtctata ttgataatgt tgttatgaag aaaacaagtt
     2581 ctgatgaagt tgaaggaaat ttacttctaa atggcgtatt taacagcttg ttaagctgga
     2641 aatatgaagc atatgatccc ggatcggcac tatttgataa tcctgatgaa cagtttaggg
     2701 cagatattag ttctgtgggt actgccggtt ggaatgtaca attgtatcag gataatgtgc
     2761 cgctggaaga aggacagacc tatgaagttt cctttagtgc aaaatcaaca gttgacagaa
     2821 aggtattaat tcagttgcaa gaagtagatg tctggacaca atacttccta tcaggaagct
     2881 caaattacag gtgatcttca gacatataca tatgaattta caatggataa gccgactgat
     2941 tcggcatcaa ggtttagtat tgcactggga agtcccgatg acggtaagac cacttatgat
     3001 ccacatgagg taataattga caatgttgtg gttagaaagg ttgtgccggt tatttcgtta
     3061 atacaaaacg gaacctttga aaatgataaa gagcattggt tggaatactg gggcggaagc
     3121 tgggaagatg accctgcagg tgccgggaat ggtactgcaa caggttcttg catagtaacc
     3181 gcaggagagc ttgaagttaa tataaccaag gtagctgtca aagaatatac acctcaaatc
     3241 aaacaggaaa acttattgct ggaaaaggat gcaacttata ttttatcctt taaagcaagg
     3301 tcacttgatg caagaactat caaagcagat attttggatt ctgtttataa ctggtatggc
     3361 ggaagtacct ttgacttaac aacggaggat gaagtctata cattcacatt tactcctact
     3421 aaggatatta gtgatggtgt tctgactata aatttcggca ccataccggg taagacttca
     3481 gtagcgacta ctatctatct cgatgatata acgataataa aacagaaata aaacagggga
     3541 cggttttact tctgatgtcc ccaattaaag aattatagat tatatgttaa aggcattatt
     3601 tgttatcata aaaataatgc ctttctttta aaaccattat taccgcaaat aaattggaaa
     3661 atgctttgta tatgcaggtt ttagactatt ggaagtatta ccgataaata tgcaaaactc
     3721 tgaaatactc cgatattaca aaaacacaat aaaattttaa ttaaaagtgg gtagtatcat
     3781 aaaaaagtgg gtatattttt ataaagtcaa ttatataaaa aaactcagat acaaaaaata
     3841 aacactagga ggtacttatt atgtcattga ttggaactga agttaaacca tttacctcag
     3901 aagcttatca taacggagag tttataacgg taacggagca agattttaaa ggaaagtgga
     3961 gtgttgtatg cttctaccct gcggatttta cttttgtttg ccctactgaa cttgaagact
     4021 tacaaaacaa ttatgaaaaa ttaaaagcat tgggagcaga agtctattct gtttccaccg
     4081 atacacattt cactcataag gcatggcacg atacatcgga ggctataaag aaaattactt
     4141 atataatgat tggagaccca tcgcataatc tttctagaaa ctttgatgtt cttgacgaaa
     4201 caactggatt ggcagataga ggtactttca ttatagaccc tgatggtgtt atttaatcgg
     4261 ttgaaatatc ggcgggtaat atcggacgtg atgccgatgt tctggttgat aaaataaagg
     4321 ctgctcagta tgttcgaaat cattccggtg aagtgtgccc ggctaaatgg aaagaaggcg
     4381 gttcaacact taaaccaagt attgatctgg tcggaaagat ataagaggta acagacatgc
     4441 ttctggatgt agaaatcaaa cagcaactgg aacagtattt ggcgttgctt gaaaatgata
     4501 tcgtcattaa ggttagtgcc gggaatgata aagtgtccac cgacatggtc gtactgatag
     4561 atgagattgc tagactttcg tcaaagatac atgttgaaaa agcggaattg gacagaactc
     4621 cgagcttcgc aatcagccgc ttgaatgagg atacaggaat tgtttttgcc ggtattccct
     4681 taggacatga gtttacttcc ttaattttgg ctctcctcca agtaggagga agagccccta
     4741 aggtagatga aaccaatatc caacggattg agcagttaaa gggagagtat cgttttgaaa
     4801 cctacattag cctaagctgt cataattgcc ccgatgtagt tcaagcactc aatataatga
     4861 gtgtggtcaa tcccagtatt acccatacca tgattgatgg ggctgtcttt aaagaagagg
     4921 ctgaaagtaa ggatgttatg gcagtgcctg cgatatacct taatggcaaa ttctttgata
     4981 acggacgct
//
LOCUS       DAOOJC010000143         4977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_13411_length_4977_cov_4.081065, whole genome shotgun sequence.
ACCESSION   DAOOJC010000143 DAOOJC010000000
VERSION     DAOOJC010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13411_length_4977_cov_4.081065"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            511..3759
                     /locus_tag="PLA01_05180"
     CDS             511..3759
                     /locus_tag="PLA01_05180"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPD00725.1"
                     /translation="MKKIVSWCIVLTIAFGLMFTPNSNYPVYAGGLNDSIFEDIVGHW
                     AQEYIEKFYGRKWVVGYEDGLFRPDRYVTRAEFTAMVVHIFKGEIKPGDSVFSDVIEN
                     DWFYDVVNYANSEGLIKGYEDGTFRPRNNMSRQDAAVLVSNLFDVDFFTGAQEYKFTD
                     EDTFPEYSYQSIKNLASHEIVKGYPDGTFRPSNLITRAEAVRMLDVVLKYIEIPSPEI
                     PEAPPVTPSPDTTSTSTPTPTPTPGNTSNGNKGVSKSTPKATPVPEILDIDLKPVKVN
                     IADTMTDPQTLQISGRAIVEIENGGNDPVKKTFEVTIFEDTNLSGGYDKNDNILGVKK
                     VRDSVYGASINVSVEVKGTVLFRDNPLYAFVDSRDVVVETNEQNNQLISNAGCENKPS
                     VGSFDPVLEWKWDTSEVFPNSINVLMTPVIMDMDGDTIPDVVFGSTESTGGNQVEVGI
                     LRAISGDSGKEIFSVTDSVYRINVAFSIAAADIDIDGKPEIIACDTTGQTLIAFENDG
                     TFKWRSPQLQSINWGAPSIADIDNDGVPEIIIGNQVLNSDGTIRWTGTGGKGGKAFGS
                     SGPLSFAVNIDMDDELEIICGNTVYKADGSILWQNNGLYDGHNAVANFDDDLYPEIVL
                     VGNGYLWLLEHDGEVKWGPVTIPGGGRGGPPTVADFDNDGKVEIGVAAAARYTVFNDD
                     GTVLWSSVTQDKSSNVTGSSVFDFEGDGSYEVVYRDELKLRIYSGMDGTILFEIPMSS
                     CTWYEYPLVADVDSDGNAEIIVVANNNCGYGIQRGVYVFGDANDTWVNTCKVWNQHAY
                     SITNINSDGTIPSKSINNWEIFNNFRCNQSIDALACVDLTMSYLRITPTDDSYTLTAR
                     IGNAGTLYAPKGVNVSFYSSPLTAEEQNSDETRELIATAIMENQINPGEFKDISVVLQ
                     RQLSGNHVIYAVVDDGSGHGMVREIDETNNTTKCEFSFDSGSYTPTPTSTPMPTPTDE
                     SVQIKDIDPAEAFDLIKNTLDEEIVILDVRTLQEFEEGHIENAINADVNSDLFNDMIS
                     SLDKNKVYLVYCASGTRSIKAANMMKEKGFHEIYNLNGGITRWKNDGFQVTE"
     gene            complement(4299..>4977)
                     /locus_tag="PLA01_05185"
     CDS             complement(4299..>4977)
                     /locus_tag="PLA01_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003522420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00726.1"
                     /translation="TDFSYCFSKCSNLTEIPEALFANNINVAHFDYCFSGCSNLTGLA
                     PSLWLRTNVTKYNTCFYGCTKLSNYEDIPYDWKGKYEEPTKIEMTLGSFMTSGPNFVM
                     TGSVINSFIRFKTKEVVSVDLIIQQDTYTAKIIASDHDMVNLQGIILNGEPNVVVICN
                     FPYIDENRNLGIDLKISNQSGKMVTISLADKNNAVRITDRDGSVISGYSESENVMIVN
                     KTKSGTN"
BASE COUNT         1600 a          805 c         1020 g         1552 t
ORIGIN      
        1 tctttataca actttcccaa tccgttttca atatttaatt tatcgctaaa aaatgggcaa
       61 cctgctaaat attcgcattg catagaactc actcctgtct cggcttgaaa tattacataa
      121 tgtatatcgg aaaaataaag gcaattatta gaatgaacaa ctaatttttt tctatttcat
      181 aatgcatact tctactggca caaagtttac tgagcacaaa atatatgaat cttatgtagt
      241 aagaccattt tgacgtttag tgtagcttta taacaaatta tctcttgcaa atatatgtga
      301 aaagtaaaat tgaaaaaaca attgtaagtg aatcttaata tgggttataa ttaaagccat
      361 aatgctgtcg ttcttcttca gtatcaaact cagttttgtt ttgaaaaaaa agatttcttt
      421 gttttcatga aaaatgatga gatctattat atatattttg tttagatgtg gtgtaataag
      481 tttacactaa agtttagggg agtgaaatgt atgaagaaaa ttgtttcatg gtgtattgtc
      541 ctgacaatag cttttggatt aatgtttaca ccaaattcca attatcctgt atatgcaggc
      601 ggcttaaatg acagtatttt tgaagatatt gttggccatt gggcacagga atatattgaa
      661 aagttttacg ggagaaaatg ggttgtcggg tatgaggatg gtttatttcg gcctgataga
      721 tatgtaaccc gtgctgaatt tacagcaatg gttgtacata tatttaaagg ggagatcaaa
      781 cctggtgaca gtgttttttc cgatgtaatt gaaaatgatt ggttttatga tgttgtaaat
      841 tatgctaatt cagaagggct tataaaggga tatgaagatg gtactttcag accgcgaaat
      901 aatatgagca ggcaggatgc ggcggtactt gtttcaaatc tctttgatgt tgatttcttt
      961 accggtgccc aagagtataa atttacagat gaagacacat ttccggaata ctcatatcag
     1021 agtataaaaa accttgcatc ccatgaaatt gtcaagggat atcctgacgg gactttcaga
     1081 ccttctaacc ttataacaag ggctgaagca gtcagaatgc ttgatgtggt tttgaaatat
     1141 attgaaatac cttcacctga aattcctgag gctccacctg ttactccaag ccccgatact
     1201 acttctacct ctacacctac tcctacacct actcccggca atacaagtaa tggtaacaaa
     1261 ggagtatcca aatcaacacc caaagcaacg ccggtgccag agatactgga tattgatctt
     1321 aaacctgtga aggttaatat tgctgataca atgacggatc ctcagactct tcagataagc
     1381 ggaagggcaa tagttgaaat tgaaaatggc ggtaacgatc ctgttaaaaa aacgtttgaa
     1441 gtgacaatat ttgaggatac aaatcttagc ggtggatatg ataaaaacga taatatatta
     1501 ggagtaaaaa aagtacgtga ttctgtgtat ggagcaagta taaacgtatc tgtggaggtc
     1561 aagggaacgg tactgtttag ggacaatcca ctgtatgctt ttgttgacag cagagatgtt
     1621 gttgttgaaa caaatgagca aaacaaccag cttatcagca atgcgggctg tgagaataag
     1681 ccttcggtag gaagctttga ccctgttctt gaatggaaat gggatacttc tgaagtgttc
     1741 ccaaattcaa taaacgtttt gatgactccg gttattatgg atatggacgg tgatactatt
     1801 cctgatgtgg tgtttggatc taccgaaagt actggaggaa atcaagtgga agttggtata
     1861 ttaagggcaa taagtggaga cagcggaaaa gagatcttta gcgtgacgga tagtgtttat
     1921 agaatcaatg ttgcattttc aatagctgct gcggatatag atattgacgg aaagcctgaa
     1981 ataattgctt gcgacaccac gggccaaaca ctgattgctt ttgaaaatga cggaaccttc
     2041 aaatggagaa gccctcagct tcaaagtatc aattggggag caccgtctat tgcagatatt
     2101 gataacgatg gagtacctga aataataatt ggtaatcaag tattaaacag tgacggaact
     2161 atccgatgga caggaacggg aggaaaggga ggaaaggcgt ttggttccag tggtccgctc
     2221 tctttcgctg taaatatcga tatggatgac gagcttgaaa taatttgcgg aaatacagta
     2281 tacaaagccg atggaagcat tttgtggcaa aataatggat tatatgacgg acataacgca
     2341 gtggcgaatt ttgatgatga tctttatcct gaaatcgtat tggtaggaaa cggatatctc
     2401 tggcttttgg agcatgacgg agaagtcaag tggggacctg tgactattcc tggaggcgga
     2461 cgtggaggcc cgcctacggt tgcggatttt gataatgacg gaaaggtaga aataggggtt
     2521 gctgcagctg caagatatac cgtatttaat gatgatggaa ctgttctttg gtcttcggtt
     2581 actcaggata aaagttcaaa tgtaacagga tcatcggtgt ttgattttga gggtgacggt
     2641 tcatatgaag tagtatacag agatgagctg aagctgagaa tatacagtgg aatggacgga
     2701 actattctgt ttgaaattcc catgagctca tgtacatggt acgaataccc ccttgtagct
     2761 gatgtagaca gtgacggaaa tgcagagatc attgttgttg caaataacaa ctgcggctat
     2821 ggtatacaaa gaggagttta tgtattcgga gatgccaacg atacctgggt taatacttgt
     2881 aaggtctgga atcagcatgc ttatagcata acaaacatta acagcgatgg aaccatacct
     2941 tctaagtcca ttaataattg ggaaatattt aataacttca gatgcaacca gtcgatagat
     3001 gctcttgcat gtgttgacct gacaatgtca tatttgcgga ttactccaac cgatgattct
     3061 tatacactta ctgcacgtat tggaaacgcc gggacattgt atgctccgaa gggcgtaaat
     3121 gtttcctttt atagcagtcc tctgactgct gaggagcaga attcagatga aacgcgagaa
     3181 ttaattgcta ctgctataat ggaaaatcag ataaatccag gtgagtttaa ggatatatcg
     3241 gtagttttac aaagacagtt gtcaggaaat catgttattt atgcagtggt tgatgatgga
     3301 tccggacacg gaatggtgcg agagattgac gagacaaata atacaacaaa gtgtgaattt
     3361 tcgtttgata gcggctctta tacaccgaca ccaacttcaa caccaatgcc gacacctact
     3421 gatgaatctg tccagatcaa ggacatagat ccggctgagg catttgattt aattaaaaat
     3481 acattagacg aagaaattgt aatacttgat gtacgtacat tgcaagagtt tgaagagggg
     3541 catatagaga atgcaatcaa cgcagatgtg aattcggatt tgtttaacga catgattagt
     3601 tccttggata aaaataaagt atacttggta tattgtgctt ccggtacacg aagtataaaa
     3661 gctgcaaaca tgatgaagga aaagggcttt cacgaaatat acaatttgaa tggtggtata
     3721 acaagatgga agaacgacgg ttttcaggtt acagaatgac accgaaatgt agttgatatc
     3781 agtactatat taatagccat acaggttttc agtctgtatg gctatatgtt tttattcatt
     3841 tgttgcttgc ccggcctctt cattctgcat atcataaaac cggcattctg tccggctatt
     3901 ttgatgatat actctcaaaa tagaagtcaa agaatagcat ttttaatttt taccttcttc
     3961 ccatatattg ataatttcgg gcaattgact gcttatagca tgtttaactg catctaaatt
     4021 cacaaattat ttttatggaa tgaccatgtt taaagttaat agtttctgaa aattatttgt
     4081 tgtatttatc atcgttttta tgatacagaa atacagaatt atttctttat aaaaattatt
     4141 gaaggataca caaacaagca tcagctcttt gtatatcctt caaatgaaat taataatgca
     4201 tcaatcctct ttattgtatg agttatatta gttaataaac aatgtttacc gataaaattt
     4261 gattacagct tacattttca ttctatgcta tagctttatt agttggttcc tgacttcgtc
     4321 ttgtttacaa tcatcacatt ttcactttca ctatagccac ttataacaga accatctctg
     4381 tcggttatac gaacagcgtt atttttatcg gctaacgaaa tagtaaccat tttcccggat
     4441 tgattcgata tcttcaggtc aattcctaaa tttctatttt catctatata tgggaaatta
     4501 catattacta ctacattggg ttctccattt aaaataatcc cctgaagatt aaccatatca
     4561 tgatcagaag ctataatctt agcagtatat gtatcttgtt gaataattag atcaacactg
     4621 acgacttcct tagtcttaaa ccttataaaa ctatttatga cggagccagt cattacaaaa
     4681 tttggaccag atgtcataaa actcccaagt gtcatttcga ttttagtagg ttcttcatat
     4741 ttgcctttcc aatcatatgg aatatcttca tagttggata gtttagtaca gccataaaaa
     4801 caagtgttgt actttgttac atttgtacgc aaccataagg acggagctaa gcctgttaga
     4861 ttactgcatc cagaaaaaca ataatcaaaa tgtgctacat tgatattatt agcaaataag
     4921 gcttccggaa tttctgttag gttgctgcat ttagaaaaac aataactaaa atctgtt
//
LOCUS       DAOOJC010000144         4928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_13564_length_4928_cov_3.220193, whole genome shotgun sequence.
ACCESSION   DAOOJC010000144 DAOOJC010000000
VERSION     DAOOJC010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13564_length_4928_cov_3.220193"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..2492)
                     /locus_tag="PLA01_05190"
     CDS             complement(57..2492)
                     /locus_tag="PLA01_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPD00727.1"
                     /translation="MKKIVSLLCILVLLTGVLGAFSVNAAPAAEEFQASGAGILNANA
                     DGIMRDISALDFVKEMRIGWNLGNTMDAPTETAWGNPRTTKSMIDKVKEMGFNAVRVP
                     VTWNTHIGPAPNYTIDQTWLNRIEEIVNYVLDNNMYVILNVHHDDWIIPTYANEEQSK
                     EKLTKLWEQIANRFKNYSDYLVFETMNEPRAIGSPSEWTGGTYENRDVINKFNLAVVN
                     TIRSSGGNNTERFIMVPTNAATGLDVALNDLIIPNNDSRVIVSIHAYSPYFFAMDIKG
                     TSNWGSDYDKTSLSNELDAIYNRFVKTGRAVIIGEFGTIDKNNLSSRVAHAEHYAKEA
                     AARGITVFWWDNGYFGPGNSDSYAILNRSGLSWYYPEIAEALVRGAGGKQNTPTPTTK
                     PSPTPTHTPSPTKSPTNDFIYGDIDGSGQVDSTDLTILKRYVLHKLFTFPYDKGELAA
                     DVNADNSIDSTDITLMKRYILRRIDKFPADDTIIPDNPGFLYNGRFDFSDPQGPKCAW
                     SGSNVELNFSGTEASVTLKSTGENWFQAIVDGNVLQPFMVSSTSTVKLVTGLSNGNHH
                     LVLWKRTEASQGEVQILGFDFGQGKLLAPPAPLERKIEFIGDSITCAYGNEGLSKDQP
                     FTPKNENSYLSYAAITARSLNASANIIAWSGIGLTMNYGGSGGPLMPDRYPFTLPQSG
                     VQWDSKNYVPQVVVINLGTNDFSTTPADKTKFITAYKELVAKVRTNYPDSHIFCCVGP
                     MLWGSGLDSCRSYVKEVISDFNSRGDSKVYFVEFPQQDPSNGYGEDWHPSLITHELMA
                     DQLTSEIKDKLGW"
     gene            complement(2879..4270)
                     /locus_tag="PLA01_05195"
     CDS             complement(2879..4270)
                     /locus_tag="PLA01_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HPD00728.1"
                     /translation="MLLTVILSSFSINTVNGAGYPYGVSSAAENQSAAEQLLASEWQS
                     WKDTYVTSSGAGGFKRVQRDPATNFDTVSEGMGYGLILAVSFNEQQLFNDLFGYVKKY
                     YNSNGLMGWHIDASGNIVGTGGKDAATDADEDIALALVFAHKKWGSSGAVDYENEAKT
                     LINNIFNHMVERGTYVLKPGDTWGGSNITNPSYFSPAWYRVFAEFTGNQEWIKVADKC
                     YDIVNNIKKYNNNTGLVPDWCTADGTPASGMGYDFYYDAIRYGWRTAIDYSWYGTSQA
                     KENCDAITNFFKKAGVSQIKDGYTITGNTTGQWHTATFTSCAAAGAMTSDLSTAIEFY
                     NECVKVKDSGSYHYFGNSLRMMILLYITGNFQNLMSGTSPTSTPTTTPTPTPTSTSSY
                     NFKYGDVDGSGQVDSTDLTILKRFILHKLNSFPYDKGHLAADVNADNSIDSTDVTLMK
                     RYILRKINKFPAE"
BASE COUNT         1456 a          985 c          887 g         1600 t
ORIGIN      
        1 aaaaagacgt atacattata ttaaaaaggc ctccacttat cgtgaaggcc aaatttttac
       61 catccaagct tgtcctttat ttctgaagta agctggtctg ccatcaattc atgggtaatg
      121 aggcttgggt gccaatcttc tccataaccg ttgctggggt cttgttgagg aaactctaca
      181 aaataaacct ttgaatctcc tcttgagtta aaatcactta taacttcttt aacataactg
      241 cggcatgaat ctaaaccact tccccaaagc attggtccaa cacagcagaa tatgtgggaa
      301 tccggatagt ttgtacgtac ctttgcaaca agctctttat aagccgttat gaactttgtt
      361 ttatcggctg gtgttgttga aaaatcgttt gtgcctaggt taataactac cacttgaggg
      421 acgtagtttt tagaatccca ctgaactccg ctctgtggaa gagtaaaggg ataacggtca
      481 ggcataagag gaccaccgga accaccgtag ttcatagtaa gacctattcc cgaccaggca
      541 attatatttg cacttgcatt caatgaacgt gcagtaattg cggcgtaaga caaataactg
      601 ttttcatttt taggtgtaaa aggctgatct ttgctcaatc cttcattccc ataagcacat
      661 gtgatggaat ctccaatgaa ttctattttt ctttcaagag gagcaggggg tgctagaagt
      721 tttccctgac cgaaatcgaa tccgaggatc tgaacttcac cctgagaggc ttctgtacgt
      781 ttccataaaa caagatggtg gttcccgttt gaaagaccgg ttacaagttt aacggtcgat
      841 gtgctgctga ccataaaagg ctgtaaaaca ttaccgtcta caatagcttg aaaccagttt
      901 tcaccggtag atttgagggt tacactggct tcggttcctg aaaaattcag ctcgacattg
      961 cttccggacc aagcacattt tggaccctgt gggtctgaaa aatcaaacct tccattatat
     1021 aaaaaccctg gattgtctgg tataatagta tcatctgcag ggaatttgtc aattcttctt
     1081 aatatatatc ttttcattaa tgttatgtct gtggaatcta tactgttatc tgcattaaca
     1141 tcggcggcta attcgccttt atcgtaaggg aaagtaaaca atttgtgaag tacatatctt
     1201 tttaatattg tcaaatctgt agaatccacc tgtccgcttc cgtctatatc accatatata
     1261 aaatcatttg ttggtgattt agtaggtgat ggcgtatgtg ttggagtagg tgatggcttt
     1321 gttgttggag tgggtgtgtt ttgttttccg ccggcacctc ttacaagagc ctcggcaatt
     1381 tcaggataat accatgaaag accggatctg tttaatatgg cataggaatc tgaattgcca
     1441 ggtccaaaat aaccgttatc ccaccagaaa acagtgattc ctctggcggc tgcttcttta
     1501 gcatagtgct ctgcatgggc tactctcgaa gaaagattat tcttatcgat tgttccgaat
     1561 tctcctataa tcacagctct gccggttttt acaaacctgt tataaattgc atcaagctca
     1621 ttggaaaggg aggtcttgtc gtaatcactg ccccaattgg aagttccttt aatgtccata
     1681 gcaaagaaat atggtgagta agcatgaatt gatacaatta ccctactgtc attattcgga
     1741 ataatcaggt catttaatgc aacatccaga ccggttgcag cattggttgg aaccataata
     1801 aatctttcgg tattatttcc tccagaactt ctaatggtat tgacaactgc caggttaaat
     1861 ttatttatta catcgcggtt ttcatatgtt cccccggtcc attcggacgg tgaaccgatt
     1921 gctcttggct cattcattgt ttcaaaaacc aagtaatcgc tgtagttctt aaaacgattt
     1981 gcgatttgct cccagagttt tgtgagcttc tctttacttt gttcttcatt agcatatgta
     2041 gggattatcc aatcgtcatg atgaacattt aaaataacat acatattatt gtcaagaacg
     2101 taatttacta tttcttctat cctgttaagc catgtttgat cgattgtata gttaggagca
     2161 ggcccaatgt gggtattcca ggttacaggt actctcacgg cattaaaccc catttctttt
     2221 actttatcta tcattgattt agtggtccgg gggtttcccc atgcggtttc ggtaggggca
     2281 tccatggtgt ttcccaaatt ccaaccgatt cgcatttctt tgacgaaatc caatgccgaa
     2341 atgtcccgca tgattccatc tgcgtttgca tttaaaattc cggctccgga tgcttgaaat
     2401 tcttctgccg caggtgcggc attaaccgaa aacgcaccta gtacacctgt caatagaaca
     2461 agtatacaca ataaagaaac tatttttttc aattcaattc actcctccac aaaattataa
     2521 tattacacta ataataccta agtctctaaa taccctgcct ccatccggga aatcatagaa
     2581 atcctgatgc ttacgttcct gaacatgatt tctgctcctt cctcctccca taaagaaaaa
     2641 aataaaaagc acttttttat aatataaaat acgcaaattt tctttttttt aaggtatgca
     2701 aataaaagat agtaaggtaa aaaataatat tattttattg agaaaatgta atttgtcatt
     2761 tcacttcgtg aaattccact aaacaaagga ccttcacatt ctatgcgaag gtcttaaaac
     2821 gatgtactta cattaagata atgaaaggcg tgatgccttg gtgaatcaaa attgagattt
     2881 attctgcagg gaacttgttg atttttctta atatatatct tttcatcaat gttacgtctg
     2941 tggaatctat actgttgtct gcattaacat cagcggctaa atggccttta tcgtaaggga
     3001 aagaattcaa tttatgaagt ataaatcttt ttaatattgt caaatctgtt gaatccactt
     3061 gtccacttcc gtctacatca ccgtatttaa aattatacga tgaagttgat gtaggagtag
     3121 gagtgggtgt agtagtagga gtcgaagttg gggatgtgcc ggacatcaaa ttctggaaat
     3181 taccggttat gtacagtaat atcatcattc tcaatgaatt tccaaaataa tgataactgc
     3241 cactgtcttt cacttttacg cattcattgt agaattctat tgcagttgag agatccgatg
     3301 tcattgcacc tgctgcggca caagaagtga acgtagcagt atgccactgg cctgtagtat
     3361 taccggttat tgtgtatccg tccttaattt gggatactcc agctttctta aagaagtttg
     3421 taatagcatc acaattttct tttgcttggg atgttccata ccaactgtaa tcaatagctg
     3481 ttctccatcc ataacgaata gcatcataat aaaagtcata acccataccg gaagcaggag
     3541 tcccgtcggc tgtacaccaa tccggtacta atcctgtatt gttgttatat ttttttatat
     3601 tattcacaat atcatagcat ttgtctgcaa cctttatcca ttcctgattc ccggtaaatt
     3661 cagcaaaaac tctgtaccaa gccggtgaaa agtaagaagg attggttatg ttagaaccgc
     3721 cccatgtatc accgggcttt aatacatagg ttccccgctc aaccatatgg ttgaaaatgt
     3781 tgttaataag tgtcttcgct tcgttttcgt agtctacggc accgctggaa ccccactttt
     3841 tatgggcaaa aaccaaagcc aatgcaatat cttcatctgc atctgtcgca gcgtccttgc
     3901 ctccggttcc gacaatatta ccacttgcat ctatatgcca tcccataagt ccgttggaat
     3961 tatagtattt tttaacatat ccgaataaat cattgaaaag ttgctgttca ttaaaagata
     4021 cagctagaat caagccatat cccataccct ctgaaacggt atcaaagttt gttgcagggt
     4081 ctctttgcac tcttttgaag ccccctgctc cacttgaagt gacataagta tctttccatg
     4141 actgccactc tgatgcaagc aattgttctg ctgcagactg attctctgct gcagagctaa
     4201 cgccgtacgg atatcctgca ccattaacag tattaatgga aaaagagcta agtataaccg
     4261 tcaataacac aagtgtacac gataaagaaa ctattttttt caaattcaat tcactcctcc
     4321 acaaaataaa tgattctttg cccaaccttt acttaacgac tagtaaaagt cggtacttta
     4381 gtacaactaa ataaaccgcc ataaattaat taagtttatg atatagtggt ttataagctt
     4441 ggacaacgtt tactgttcga atattatgtt gtccggtatt atgggttgtt tataatgtaa
     4501 aatatactaa ttacgttcag atatcgaata accgccccct tttcaaaaca taataagctg
     4561 tgttgctttg cctgagttta tgagcactgc ctaactgttc ctcctccttc catgaaacaa
     4621 tagcctaata aaaatacttt atatatagta aattcgtaag acaccaatta tttagggtgg
     4681 tatcttacga aactaattat gatcatactg aaaatctatg tttatatcga aataaaaatg
     4741 tcataatgta acaggttata tttcacgtgc tttgaaatgg cgaattgagc cataggacta
     4801 atttcccgta aaaaggatta ataaaaaacg gttatttagt cctatttaat tccgatataa
     4861 agaatagtat aattaaagaa taactctaga ttttttgagt tatataggat tttctgtttt
     4921 atgtcaaa
//
LOCUS       DAOOJC010000145         4924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_13580_length_4924_cov_4.293284, whole genome shotgun sequence.
ACCESSION   DAOOJC010000145 DAOOJC010000000
VERSION     DAOOJC010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13580_length_4924_cov_4.293284"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLA01_05200"
     CDS             complement(<1..410)
                     /locus_tag="PLA01_05200"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513828.1"
                     /note="catalyzes the formation of L-citrulline from
                     carbamoyl phosphate and L-ornithine in arginine
                     biosynthesis and degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPD00729.1"
                     /translation="MKHLISLHDLSVEEIESILKLAEKLKRQLKEGVPHQLLKGKTLG
                     MIFTKSSTRTRVSFEVGLYQLGGYPLFLSSNDIQLGRGESIYDTAQVLSRYIDGIMIR
                     TFAHSDVEDLAKYGNIPVINGLTDLMHPCQILADL"
     gene            complement(560..3763)
                     /gene="carB"
                     /locus_tag="PLA01_05205"
     CDS             complement(560..3763)
                     /gene="carB"
                     /locus_tag="PLA01_05205"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPD00730.1"
                     /translation="MPKIEKIKKVLVIGSGPIIIGQAAEFDYSGTQACKALREEGIEV
                     VLVNSNPATIMTDTQSADRVYIEPITLDVLKKIIKEEKPNGILASLGGQTGLNMAVEL
                     AEDGILDEMGIELLGTSLESIKRAEDRELFKKTMQEIGEKVPLSTIVTDLESGVKFAE
                     EVGFPIIIRPAYTLGGTGGGIAHDMEEFKYICGKGLKLSMIHQVLLEQSVAGWKEIEY
                     EVMRDGADNCIIICNMENFDPVGVHTGDSIVVAPCQTLSHVEAKMLRKASIKIIRALN
                     IKGGCNIQFALNPNSFEYVVIEVNPRVSRSSALASKATGYPIARVTSKIAIGLNLDEI
                     KNSVTQNTYACFEPSIDYVVTKVPRWPFDKFINADRSLGTQMKATGEVMAIGRTFEES
                     ILKAIDSLDIKMNYQLGLSLFDNISTGELLDFIKVARDERIFALCKALQKGVSAEEIS
                     AITKIDIFFIKKLQKIVNLAEEIKNSGIAWLDYDLFARAKKMGFGDSYIANLVNVPLE
                     TVLDLRKKYAIRPAYRIVDTCAGEFKAVTPYYYSTYGENDDVVVSGKKKVIVIGSGPI
                     RIGQGIEFDYCSVHSVKTLRELGIESIIINNNPETVSTDFDTSDKLYFEPLTKECVLD
                     IINKEKPLGIIVQFGGQTSINLAGTLASEGVNILGTSVESIDIAEDRDRFMNLLEELN
                     IPLPQGDTAFSIDEAIEIAKKIGYPVLVRPSYVLGGRAMEVVYNDETLKEYMNRAVYI
                     STKHPVLIDKYIDGKEVEVDGICDGKDVLIPGIMEHIERAGIHSGDSISIYPPQTLDE
                     QIKNTIVDYTTRLAKALKIVGLFNIQFVIDRSNKVYVIEVNPRASRTIPIMSKVTGIP
                     MVDVATKFILGYKMKDLGYTPGLYKESEYVAVKAPVFSFSKLTTVDTFLGPEMKSTGE
                     VMGVSKDFHIALFKALVASGIKIPSGGSVLLSIADRDKRECVEIAQALSDLGFNLMAS
                     EGTYTNLSVLGIEVEMVPDDKMIELIKSNNISLVINTPTMGKIPGRLGFILRRTSIEY
                     NIPCITSLDTARSMISILEHMISGEDTEICTLDRYSK"
     gene            complement(3798..4856)
                     /gene="carA"
                     /locus_tag="PLA01_05210"
     CDS             complement(3798..4856)
                     /gene="carA"
                     /locus_tag="PLA01_05210"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPD00731.1"
                     /translation="MKSVLVLEDGTYFSGEAFGEAGETVGEIVFNTSMTGYQEIITDP
                     SYRGQIVAMTYPLVGNCGFNQYDTESSGSHVQGFIVKELSDTPTNWRFEVTPDEYFKK
                     NGIVGIKGIDTRTLTQHLRDKGSMYGIISTESGNIDALLERLMKKKQEKRSLVAEVTT
                     KAPVHKPGNGKRVVVIDFGVKNSIIKSLEKNNCDIYILPATSSAMEILDHKPDGILLS
                     NGPGNPAELLSIKATVQELIGKKPILGIGLGFQILGLALGGKTGKLKFGHHGCNYPVK
                     DHRTGRCHITSQSHNYVLENSFGDDVIVTQTNINDNTVEGLRHKKYPIIGVQYHPQTI
                     TEDDTSYVFYDFYKMIEE"
BASE COUNT         1262 a         1137 c          984 g         1541 t
ORIGIN      
        1 aggtctgcga gtatctggca tgggtgcata agatccgtaa gaccgtttat aaccgggatg
       61 ttgccgtatt ttgcaagatc ctccacatcg ctgtgggcaa aggtcctgat cattattccg
      121 tctatatatc ttgacagaac ctgagctgta tcataaatgg actcaccgcg tcctagctgt
      181 atatcgtttg agcttaggaa caggggatat cctcccagtt ggtataatcc cacttcaaaa
      241 gatacacggg ttctggtaga tgactttgta aaaatcattc caagggtttt accctttaac
      301 aattggtggg gaactccttc tttaagctgt cttttgagtt tttcggcaag cttaagaata
      361 gactcgattt cctcaaccga taaatcatga agacttatta aatgcttcat aaaaaacgcc
      421 tccaaatcta ataaattata tacttttaac ttcaacagaa acggtataat ctatgctgta
      481 attacatccc catcaggctc taagccaagc ctgcagaata ataatgcagt tggcattcat
      541 cgcccaaaaa ctacagaacc tacttggaat atctgtcaag agtgcatatt tcggtatctt
      601 ccccggagat catatgttcc agtatcgaga tcattgatct tgcagtgtca agagaagtta
      661 tacacggaat attatattca attgatgtcc gccttagaat aaatcccagt cttcccggta
      721 ttttacccat ggttggcgta tttattacca aagatatgtt attgctctta atcagttcaa
      781 tcattttatc atccggaacc atttccacct cgattccaag aaccgaaagg tttgtgtagg
      841 tgccctcaga ggccatcagg ttgaaaccga ggtctgaaag ggcctgggcg atttcaacac
      901 attcccgttt atcccggtct gctatggaca acagtacact tccgccggag ggtattttaa
      961 tgccggaagc aacaagtgct ttgaagagag ctatatgaaa gtcctttgat actcccatta
     1021 cttctccggt ggatttcatt tccggcccta agaatgtatc gactgtagtg agctttgaaa
     1081 aagagaagac cggagctttt acagcaacat attctgattc tttgtaaaga ccgggggtat
     1141 atccaagatc cttcattttg tatccgagga taaactttgt agcaacatcc accataggta
     1201 ttccggtgac tttgctcatt atcggaatag tgcggcttgc ccggggatta acctcgatga
     1261 catagacctt gttgctgcgg tcaatgacaa actgaatatt aaacagtccc actattttaa
     1321 gggctttagc cagccttgta gtgtaatcca caatcgtgtt ttttatctgt tcatcaagag
     1381 tttggggcgg atatatcgaa atgctgtctc ccgagtggat tccagccctt tcaatatgct
     1441 ccatgatgcc tggaattaaa acatctttgc cgtcgcagat gccgtccacc tcaacttcct
     1501 ttccgtcaat atatttgtca ataaggacag gatgctttgt tgagatatag acagcccggt
     1561 tcatatactc tttaagagtc tcgtcgttat aaaccacttc cattgctctt ccgcccagta
     1621 catatgacgg tcttaccaga acagggtatc ctattttctt tgctatctca atggcctcgt
     1681 caatggagaa ggcggtatct ccctggggga gaggaatatt tagttcctcc agaaggttca
     1741 taaaccggtc cctgtcctct gcaatatcga tactttccac cgaagtacca aggatgttta
     1801 cgccctcact ggcaagtgtt cccgcaaggt tgatggatgt ctggcctccg aattgtacaa
     1861 ttattcctaa aggcttttcc ttgtttataa tatcaagaac acactccttg gtaaggggct
     1921 cgaaatacaa tttatccgat gtgtcaaaat ccgtacttac cgtttccggg ttattgttta
     1981 taattatcga ctcaattccc agttcccgta aagtcttgac cgagtgaaca ctgcagtagt
     2041 caaactcaat accctggcct attcttatag gaccggagcc tattactata accttctttt
     2101 tgccggaaac aacgacatcg tcgttttctc cataggttga atagtagtag ggggttactg
     2161 ccttaaactc accggcacag gtatcgacaa ttctgtatgc cggacggatt gcgtattttt
     2221 ttctgaggtc taaaaccgtt tcaagaggca catttacaag gtttgcaatg tatgaatccc
     2281 caaaccccat tttctttgct ctggcaaaaa ggtcgtagtc aagccaggca atgcctgagt
     2341 ttttgatttc ctcagccaga ttaacgattt tttgaagctt ctttataaag aaaatatcaa
     2401 ttttcgtaat tgcacttatt tcttctgcgg acactccttt ctggagtgcc ttgcaaagtg
     2461 caaaaatacg ttcatccctt gcaactttta taaaatccag cagttctccc gtggatatgt
     2521 tgtcaaaaag gctcagtccc agttggtaat tcattttaat gtccaaagag tcaatagcct
     2581 tcagtatgga ttcttcaaag gttcttccaa tggccattac ttcaccggtg gccttcatct
     2641 gagtgcctaa tgacctgtcg gcgtttatga acttgtcgaa gggccaacgg ggaacttttg
     2701 taaccacata atctatggaa ggctcaaaac atgcataggt gttttgagtt accgagtttt
     2761 taatctcgtc aaggttaaga cctattgcaa ttttagacgt cacacgggca ataggatatc
     2821 ccgtagcctt ggaagcaaga gcactggagc ggcttaccct tggattgact tcaattacca
     2881 cgtattcaaa gctgttggga tttaaggcaa actgtatgtt acaaccacct tttatattaa
     2941 gagcacgaat tatctttatg gaagcctttc tcagcatctt ggcttcaaca tgtgaaagcg
     3001 tttggcaagg ggcaacaaca atgctgtcac cggtatgaac tccgacaggg tcaaagttct
     3061 ccatattgca tattataata cagttgtcgg caccgtccct catgacctcg tattcgattt
     3121 ctttccatcc tgccacgctt tgctccagca aaacctgatg tatcatactg agcttcagac
     3181 ccttgccgca gatgtattta aactcctcca tgtcatgggc gataccgccg ccggtgcctc
     3241 ctaaggtgta tgccgggcgg attatgatag ggaagcccac ttcttctgca aactttacgc
     3301 cgctttcaag gtcggttaca atggtactta agggaacctt ttcaccgatt tcctgcatgg
     3361 ttttcttgaa cagttcccgg tcttctgccc tttttatcga ttctaaggat gtgcccaaaa
     3421 gctcaattcc catttcgtcc aaaatgccgt cctcagcaag ctcaacagcc atgttcaagc
     3481 cggtctgacc gccaagggaa gctaagattc cattgggttt ttcctcttta attatctttt
     3541 ttaaaacatc cagggttatc ggttcaatat ataccctgtc tgccgattgg gtgtcggtca
     3601 ttatggttgc tggattgctg tttacaagaa cgacttcgat tccttcctct ctcaatgctt
     3661 tgcatgcctg cgttcccgag tagtcaaact cagcagcctg accgataata ataggtcccg
     3721 atccgatgac aagaaccttc tttatttttt ctatttttgg catctgaacc tccgtattgc
     3781 tattatatag attttgatta ttcctcaatc attttgtaaa aatcataaaa aacgtaggaa
     3841 gtatcgtcct cggtaattgt ctggggatga tactgtacgc cgattatagg atatttctta
     3901 tgccttagtc cttccacggt attgtcgttg atgtttgtct gggtaacaat cacatcgtct
     3961 ccaaagctgt tttccaaaac ataattatga ctctgcgatg ttatatggca tcttccggtc
     4021 ctatggtctt taaccggata attgcagccg tggtgcccga atttcagctt gccggttttg
     4081 ccgcctaacg ctaagcccaa gatttgaaac ccaaggccga ttcccaagat tggctttttg
     4141 cctataagct cctgtacggt ggcctttatt gacaggagtt ctgccgggtt gccgggaccg
     4201 tttgaaagaa gtatgccatc gggtttatgg tccaaaatct ccatggctga cgatgtggcc
     4261 ggaagaatgt atatatcgca gttattcttt tctaaagatt tgattatact gttttttact
     4321 ccaaagtcta ttaccacaac gcgtttccca ttgcccggtt tatgtacggg tgcttttgtc
     4381 gtgacctcgg ccaccaaact tcttttttcc tgcttctttt tcattagcct ttcaagaaga
     4441 gcatcaatat ttccggattc ggtagatatt attccgtaca tgcttccctt gtctctgagg
     4501 tgctgggtaa gagtacgggt atcaatacct ttgattccta caataccgtt tttcttgaaa
     4561 tactcatcgg gagttacctc aaaacgccag ttggtcggtg tatcggaaag ctccttaacg
     4621 ataaaccctt ggacatgact tccggacgac tcagtgtcat attggttgaa accgcagttt
     4681 ccaaccaatg ggtaagtcat ggctactatc tgacctctgt aggaggggtc ggttattatt
     4741 tcctgataac cggtcatgct tgtattgaat acaatctctc ctactgtttc tccggcctca
     4801 ccgaaggcct cacctgaaaa atacgttcca tcctccaaaa ctaatactga tttcatgttt
     4861 ctatctcctc aaatattaat attctcgaaa tatattatct aatacgttga ccatgccgtc
     4921 aatg
//
LOCUS       DAOOJC010000146         4915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_13610_length_4915_cov_2.820988, whole genome shotgun sequence.
ACCESSION   DAOOJC010000146 DAOOJC010000000
VERSION     DAOOJC010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13610_length_4915_cov_2.820988"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1367)
                     /locus_tag="PLA01_05215"
     CDS             complement(<1..>1367)
                     /locus_tag="PLA01_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPD00732.1"
                     /translation="LKDMIEQSSSLFADKNAFHIKSKGDKYEGIKYIDFKNDIDAFGT
                     ALLELNLKDSFIAVIGENRYEWCVTYLSVINGVGVIVPLDKELPVSELENLLNGSGAR
                     SIVYSGRLFHDKLKQISSKLSNIKYYINMDTEEHESNSFLSFKALIKTGRELLSSGVK
                     DYINSEIDENALGALIYTSGTTDYAKGVMLSHKNICSNIMSVCSSLYIDSSDSILSIL
                     PLHHTYECTAGFLTMIYNGVTISFNEGLKYISKNLQETKPTILILVPLILENMYKKIW
                     TQAEKKKSLKIKLKVGLLISNFLYNTFKIDIRKKLFKQILDNVGGRLRLVISGAAAID
                     PNVSKGLSDFGINIRQGYGLTEASPIVSVNRDKEFRDDSAGLPLPGLDVQINNPDHEG
                     LGEIIVKGESVMLGYYNNDAATKAVLKDGWLFTGDLACMDEKGFIYITGRQKNVIVTK
                     NGKNI"
     assembly_gap    1368..1377
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1633..2931)
                     /locus_tag="PLA01_05220"
     CDS             complement(1633..2931)
                     /locus_tag="PLA01_05220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HPD00733.1"
                     /translation="MKKLLLIVTIIIIVFSNAVYINAQPLDINARAYILIDSKTGQVL
                     AEHNPDFKTYPASTTKIMTGILAIELGDLDQVMTVNQSAIDDIGPGGMHIGLLAGEQL
                     ELRYLLDALLVRSANETAYVIAENLCSTRQEFYDLMNERARELGATNTNFVNPCGIDN
                     GKKGENHLTTARDLAIIARYGMTLPYFREVVKKTLITIPPTNKHDEEVIVGSTNKLLL
                     YASSKYKSDYYTRITGIKTGYTDRALNNLVSSAENDEGIELISVILGVENYDMVFDYS
                     KELLEYGFKNYSMQQAVAPNSYIKSVPVANAAGNNNLDILASPEGLKCLLPNNASKAD
                     YEVEEHINSDITAPVKKGDVLGYLEIKRNGVSLGKVNAVASRDMEILMPSPTPAGILE
                     QTINSSVLKKVTLGTLIFFIMFMLLRFTLRKISRSINSKR"
     gene            3119..3877
                     /locus_tag="PLA01_05225"
     CDS             3119..3877
                     /locus_tag="PLA01_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPD00734.1"
                     /translation="MLRDFFNQEINMKKSVVALMILGLVVTTSVTGYLLANQNSGIII
                     SKDDSNSRNNMTQAADSNAENNGGNEINEGIDRQDEEPVEEIKVYVVGEVNNPGVVTL
                     KKGQIISDAIELAGGPTEKADIENINMVYELEENVMLRVWSKEESMKDGISKDNQAAE
                     NDGNKGISIVKDSAGVVVEEKDNKDGKVSKININTASENELLSLPGVGPSTAAKIISY
                     REQNGEFKKIEDIMNVSGIGEAKFNQMKDFITVK"
     gene            4140..>4915
                     /locus_tag="PLA01_05230"
     CDS             4140..>4915
                     /locus_tag="PLA01_05230"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPD00735.1"
                     /translation="MRKILVLVTTLLAVVLLLSGCNVPWGSDNGDSGGGQPNNGNGGQ
                     DNKTEVTLYFPSKDNSRVVEEKRKIAIDGGAIIKSCVEALIQGPENGELRKAFPDGTK
                     LLSANTVGDVAVVNFSKEYENTNGLDELLLRVTLVNTLTEIEGVEKVRIMVVGQELIG
                     PSGEAFGDMARIPLDSEGKPKMNDETTVVVYFSDADSEKVVAEERTVELTEGDRIEKI
                     IFEELKKGPENGGLEPVIPDGTKLLSVDTKDGVCTLNLSK"
BASE COUNT         1506 a          834 c         1067 g         1498 t
ORIGIN      
        1 aatgttcttg ccgtttttag tgactattac attcttttga cggccggtga tataaataaa
       61 gcctttttca tccatgcagg caaggtctcc tgtgaaaagc catccgtctt tcaaaactgc
      121 tttagttgca gcatcgttat tgtaatagcc aagcattaca ctttcacctt ttacaatgat
      181 ttcaccgaga ccctcatgat cgggattgtt gatttgtaca tcaagtcccg gaaggggaag
      241 tccggctgaa tcatccctga attccttgtc cctgttgact gaaacaatcg gagaagcctc
      301 cgtaagacca tagccttgac gaatattgat gccgaaatcc gaaagtccct tggacacatt
      361 tggatcaata gctgctgctc cggaaattac aagcctcaac cggcctccga cattatcgag
      421 gatttgcttg aataacttct tgcgtatatc gattttaaag gtgttgtaaa gaaaattgct
      481 gattaaaagt cctactttta gctttatttt aagactcttc tttttttctg cctgagtcca
      541 tattttcttg tacatgtttt ccaatatgag gggtaccagg atgagtatag tcggcttggt
      601 ttcctgaaga tttttggaga tgtatttaag accttcatta aaggaaattg ttactccgtt
      661 gtatatcata gtgagaaaac cagcggtgca ttcataggtg tgatgaagag ggagtattga
      721 gagtattgag tcgctgctgt cgatgtaaag ggaggaacaa acactcatta tatttgaaca
      781 aatatttttg tgagacagca taacaccttt cgcataatcg gtggtaccgg aagtatatat
      841 taatgcaccc aatgcatttt cgtcaatttc ggagttaata tagtctttta ctccggagct
      901 taatagctct ctgcctgttt ttattaatgc cttaaaggat aagaagctgt tgctttcgtg
      961 ttcttcagtg tccatgttta tatagtattt aatattgctc aatttggatg atatctgctt
     1021 tagtttgtca tgaaaaagtc ttcctgagta aacgatggaa cgggcaccgg aaccgttcag
     1081 aaggttttcc agctctgaaa cgggtagttc tttgtctaac ggcacaatca ctcctactcc
     1141 gtttatgacg gaaagatacg taacacacca ttcatatctg ttttcgccaa taaccgctat
     1201 aaagctgtct tttaagttta attccaagag agcggttcca aaagcgtcaa tatcgttttt
     1261 gaaatcgata tatttgattc cttcatactt atcgccctta ctctttatat gaaatgcgtt
     1321 tttgtctgca aacagagatg agctttgttc aatcatgtcc tttaagtnnn nnnnnnnatc
     1381 ggtcttacgt caaaaatcgg cgtaaatttc ataacaacac tccttcaagg gacaagcctt
     1441 aattatttct taattctaaa ttatactata taaattatac atcaaaatat gtttattgta
     1501 atagtgaatt taaaaatagt ttgactcatt gcattgttta tgattaaacg ataacagcta
     1561 cacataaaaa ttgtcgcaat ataccggaat atgttttccg tatttaaaga ctaaaagttt
     1621 tgctgtgtat atttatcttt tagaattaat actccttgat attttgcgga gggtaaacct
     1681 caacagcata aacattatga aaaatattaa cgtacccaga gtgacttttt tcaaaacaga
     1741 gctgtttatt gtctgttcaa gtatgcctgc aggagtcggt gaaggcatta atatttccat
     1801 atctcttgaa gcaacggcat tgaccttgcc cagtgaaacg ccgtttcgtt taatttccaa
     1861 atatccgagg acatcgcctt tttttacagg ggcagtgata tcactgttaa tatgctcttc
     1921 aacttcataa tcagctttac tggcattatt gggcaataaa catttaagcc cctccggtga
     1981 agctaatata tccaaattat tgttccctgc ggcattggca acaggaacgg attttatata
     2041 ggagtttggt gccacagcct gctgcatgga atagtttttg aagccgtatt ccaaaagctc
     2101 tttggaataa tcaaacacca tatcgtaatt ctcaacaccg agtattacgg aaatcaactc
     2161 aattccctcg tcgttttccg cagaagagac aagattgttc aatgctctgt ccgtataccc
     2221 tgttttaatg cctgtaattc ttgtataata atcggatttg tattttgagc ttgcgtacag
     2281 caaaagcttg ttggttgaac cgacaataac ctcttcatca tgcttgtttg taggcggaat
     2341 tgtgatgaga gtttttttta ctacttccct gaagtagggc aatgtcatgc cgtagcgtgc
     2401 aattatcgca aggtctcttg cagtagtaag atggttttct cccttctttc cgttgtcaat
     2461 tccgcaagga ttcacgaaat ttgtattggt agcaccaagt tctctggctc tttcattcat
     2521 taaatcataa aattcctggc gagtggagca gaggttttcg gcgataacat aggctgtctc
     2581 gtttgccgat cttaccaaaa gggcgtccag caaatacctt agctccaatt gctctcccgc
     2641 aagcaaaccg atgtgcattc ctccgggacc tatgtcatcg attgcagatt gatttactgt
     2701 cattacctga tcgaggtctc ccaactcgat cgctaaaata ccggtcatga ttttggtggt
     2761 actggcagga taggttttga aatcgggatt gtgttccgcc aatacctggc ctgtttttga
     2821 atctataaga atatatgctc ttgcatttat gtccaggggt tgtgcattta tataaacagc
     2881 atttgaaaac actataataa ttatagtaac gattaacagt agttttttca ttggttcctc
     2941 cagctttata tgatgattat ataataataa aaatagatgt tttgatgttt aattctaagt
     3001 ataacaaaaa gttgcgaaaa atgtagcttt gaattaaaaa aaacctgaag gtatattaag
     3061 gaagtatgca tttacattta tgtgtaaatt cattattata gatattggag gggaaattat
     3121 gttaagggat ttttttaatc aggaaatcaa tatgaaaaaa agtgtcgttg cacttatgat
     3181 attgggattg gttgtaacta cttcggttac ggggtatctt ttagcgaatc aaaacagtgg
     3241 tataattata agcaaggatg attcgaattc caggaataac atgacacagg ctgcagacag
     3301 caacgcggaa aacaatggcg gtaatgagat aaatgaaggt atagaccggc aggatgaaga
     3361 gccggtggaa gagataaagg tttatgttgt gggagaggta aataaccccg gagttgtcac
     3421 tcttaagaag ggtcagataa taagtgatgc gattgaactt gccggaggtc cgaccgaaaa
     3481 agccgatata gagaacatca atatggtata tgagcttgaa gagaatgtta tgctcagggt
     3541 atggtcaaaa gaggaaagta tgaaagacgg aatatcaaag gataaccagg ctgcagaaaa
     3601 tgacggcaat aaaggaataa gtattgttaa ggacagtgca ggggtggtgg tcgaagaaaa
     3661 agacaataaa gacggtaaag tctccaagat aaatataaac acggcttcgg aaaatgaact
     3721 tttgagcctt ccgggtgttg gaccttccac tgctgccaag atcatatctt atagggagca
     3781 aaacggtgag tttaagaaaa tagaagacat aatgaacgtt tcgggaatag gggaggctaa
     3841 atttaatcaa atgaaggatt ttatcacggt taaatgagtg ggatagcgtc acaatggaat
     3901 aaaatgtatt agacgaagga aatgcggttg gagtataatt ataagacata atcattgtat
     3961 gagtttattt caatgctaaa gacaaaggag gtgacttatc agactgcagt acaatattgg
     4021 atatcgtata agtttttttg gcaaaatcaa cagggctaca gctgttgaac gaatttaaaa
     4081 gaaaaagctt gtattgcttt ttatgtccaa tgaaataaac cggtctgata agaacattta
     4141 tgagaaaaat tttggttctg gtaacaacat tgctggcagt tgtgttatta ctaagcgggt
     4201 gtaatgttcc atggggttcg gataacggtg attccggcgg aggtcagcca aacaacggaa
     4261 acggtggaca agacaataaa acagaagtga ctctgtactt tcccagtaaa gataacagca
     4321 gggtagtgga ggaaaaaaga aaaatagcta tagatggcgg tgcgataata aaaagttgtg
     4381 ttgaggcttt aattcagggg ccggaaaacg gtgaactcag aaaagctttt cccgacggaa
     4441 caaagctttt aagtgccaac acagttggag atgttgccgt tgtcaacttt tctaaggaat
     4501 atgaaaatac gaacgggctg gatgagcttt tattgagggt tactctggta aataccctta
     4561 cagaaattga aggtgttgaa aaggtcagaa ttatggttgt cgggcaggag cttattgggc
     4621 caagcggtga ggctttcggt gatatggcaa ggatacctct ggattccgag gggaagccaa
     4681 aaatgaacga tgaaacgact gtggtggttt atttcagcga tgccgattcc gaaaaagttg
     4741 ttgcagagga aagaactgtg gaactaacag agggtgacag aattgaaaaa attatttttg
     4801 aagagcttaa aaagggtcct gagaatgggg gattggagcc tgtaattccc gacggaacta
     4861 aactgctttc agttgataca aaggacggag tttgtacgtt gaatctttcc aaaga
//
LOCUS       DAOOJC010000147         4898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_13665_length_4898_cov_3.915342, whole genome shotgun sequence.
ACCESSION   DAOOJC010000147 DAOOJC010000000
VERSION     DAOOJC010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13665_length_4898_cov_3.915342"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1061
                     /locus_tag="PLA01_05235"
     CDS             111..1061
                     /locus_tag="PLA01_05235"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HPD00736.1"
                     /translation="MKLFLAPIQGMTIACYRNAFARHFGNTDAYYAPFIGLSETKKIS
                     NLLLKDLLPEHNDNSINVVPQLLGNDGVLFKKFATVICAMGYREINWNIGCPFPMVTG
                     RKRGAGILPYPDMVRKFLDTACSDSSYNISVKMRLGLKDPEEGFRVMEVLNEFPLCKV
                     IIHARTGMQMYTGQVDLDTFKALASSCRHEVTYNGDIFTYDDYLRIKSCFPEVNNIML
                     GRGALYDPFLASVIKGSNISSESKICIIRDFHDDIFKYYQTRLSGTKHLSDKMKEFWD
                     YLHVHICTDKRLLKKIKKCHDSLQYIQLVNQLFDSAQWIE"
     gene            complement(1153..3342)
                     /locus_tag="PLA01_05240"
     CDS             complement(1153..3342)
                     /locus_tag="PLA01_05240"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPD00737.1"
                     /translation="MKKKYSLWSLLVILVFLICSSYVFAENTMEQPESSDGIMNSNNL
                     SNTGHINLENEYINVAINPNGRFTIGTNEGNPEIATDNGKKLLYGWQGGDTSYTTVRL
                     DGTNYYYGGENIATPPHNIDEEYRNSSVAKYGNVYVRQDIRLVDGGFSGINDTVEIKY
                     IVTNNDTVPHDAGIRIMLDTMLGSNDSAPFRVPGIGDIVTTTTFLGDKIPDYWQAMDS
                     LVEPTVVSQGTNVKYQQNPPDKFVLGRWRVLYDNIWNFNYSGTNGDSAVATYWEPKEL
                     APGETMEYVTYYGISKFTIASDKPLVVIVSGNTSLSATETGYVPNPFTITAYITNNTD
                     DTIKNVKAKIILPDEIVPYTGTDIDIAVGDMAAGSHRQVSWQLFAGQIPEQKTLEYKV
                     AVWGDDTESTEVERQITIPASISDIFDDGSDKSKYKVLRELGNDFEQDITSRIHLKPE
                     IKNVRIMPGNQEGVYELYVDEYFADTSKGASPFFYWASTKGYFEDKKIDNYRKVIFHC
                     DEGTSGQKVSILLKMGDGLGNVVYKKLYVDGGGVISSGTLEASFTNQVTSVKSWDTVK
                     IRYNISYTDSEGQPVPGTTVSLYYSLDNQAEWKPVAMDLVGVNGYNWIVPDLSSSNAR
                     IKLVATNGSVRKEVISQPFTITPDIYVKGKVADRNGNPIVNAKVSIGGRYTYTDVNGS
                     YTINRLLPGVHSLKVEKEGMDFEKSQYDNINLSYLNRNTTKNFYEKQ"
     gene            complement(3370..>4898)
                     /locus_tag="PLA01_05245"
     CDS             complement(3370..>4898)
                     /locus_tag="PLA01_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPD00738.1"
                     /translation="KSCIFTYHLPTQNSEAPKIAYNLRLFDGVFEFGNGRTGNNIYDL
                     EPTNNLSSTQDTIIAQNPNWRDSNIWGVFTDEREHAVRKAIESWYVIANTMVPNEFYR
                     VKSVDNDKIPKEIVEYIVNSLKRGDVLYVGIWALVGGHALLAYAQESDEELNTVTLSI
                     YDCNTPYSKDNDDWKDCKIVLTRNEGLNTYTATYIPWEESHPITEFCVMKTSELLSYM
                     YENSTYIEKVTYDADEDSYTLTTRLPVVDTRIQNYGNEDSPVCVVERINDTNFVIRPV
                     NGLGRRLRECGFQVEFNIQIHDGLTEWVTVFDHPETQLFTDVSISDWYYEYIKEVVKK
                     GYMQGTGNKRFDPDLVVTRAQIITIALNMAGIDYNNHPALGESGDPFTDVASGSWYYN
                     AVKVAKKLGYVSGYGDGSFGPNDPVQRQHVATMLVKILDLFKEPPEGYYDLPMFSDER
                     DISGGQLKDVKKIAYNRGWEGFYTLDSETPEFRPKLNITRAEFAKIIYKLTSLDEYMY
                     "
BASE COUNT         1363 a         1113 c          858 g         1564 t
ORIGIN      
        1 ttgacaaacg gaattgcttc atctccttca taatatattg tacaatgttt attgtaaaat
       61 atataacagt gtgttctaaa ttacctgact ttttttgggg gagtgggttc atgaagcttt
      121 ttttagcacc aatacagggt atgacaatag catgttacag gaatgcattt gcacggcatt
      181 tcggcaatac cgatgcatat tacgcccctt ttatcgggct ttcagaaaca aagaaaataa
      241 gcaatttact tttaaaagac cttttaccgg agcataacga caattcaatt aatgtcgttc
      301 cacagcttct cggtaatgac ggtgttctct ttaagaagtt tgccacggtt atttgtgcca
      361 tgggataccg tgaaatcaat tggaacattg gctgtccttt tcccatggtg acagggcgaa
      421 aaagaggggc cggaatattg ccatacccgg atatggtaag aaagtttctc gataccgcat
      481 gctccgacag ctcttacaat atctcggtaa aaatgaggct ggggctcaag gatcctgaag
      541 aagggtttcg tgtcatggaa gtgttaaatg aatttcccct gtgcaaagta ataattcatg
      601 cccgtaccgg aatgcaaatg tatacggggc aggtggacct ggataccttt aaagcccttg
      661 ccagctcatg cagacacgag gtaacctata atggtgacat ctttacttat gacgattatc
      721 ttcgcataaa atcctgcttt cccgaagtta ataatataat gctgggcaga ggggctttat
      781 atgacccttt tcttgcatcc gtgattaaag gaagcaacat atcctctgaa agtaaaattt
      841 gcataattcg tgattttcac gatgatattt ttaaatatta ccaaacccgg ctttcaggaa
      901 ccaagcatct gtcagataaa atgaaggagt tctgggatta tttacacgtt catatatgca
      961 ctgacaaaag gttgctgaaa aaaatcaaaa aatgccatga ctcattgcaa tatatacaac
     1021 tggtcaatca actgtttgat tctgcacaat ggattgagta acgtcgtttg ctgtatctga
     1081 aaattcggtt atataaaaaa ctcactgtgc tatttagctg taacacagtg agttttaata
     1141 accttttttt acttattgct tctcataaaa gttctttgtg gtattgcggt taagatagct
     1201 taggttaata ttatcatatt gtgacttttc gaagtccatt ccttcttttt ctactttgag
     1261 agaatgcacc cccggcaaca aacggttaat tgtataactg ccgtttacat cagtataagt
     1321 atatctgccg ccaatactta ctttggcatt gacaatcgga ttcccgttgc ggtctgcaac
     1381 tttccccttt acatatatat ccggtgtaat cgtaaagggt tgggatatta cttccttcct
     1441 tacgctgcca ttggtcgcaa caagcttaat tcttgcattt gaagatgata aatccggaac
     1501 tatccagttg tatccgttga cccctaccaa atccattgca acgggcttcc attcagcctg
     1561 attatccaat gaataataaa gacttacggt agttcccggt acaggctggc cttccgaatc
     1621 cgtataggaa atattatacc tgattttcac ggtatcccat gactttaccg atgtaacctg
     1681 atttgtgaaa cttgcctcaa gggttccgga ggaaattacc ccgccgccgt ctacatacaa
     1741 tttcttgtaa acaacattcc ccagtccgtc gcccatttta agcaatatgc tcaccttctg
     1801 cccggaagtc ccttcatcac agtgaaagat tactttccta tagttgtcaa tttttttatc
     1861 ttcaaaatat cccttcgttg acgcccagta aaagaaaggt gacgcaccct ttgaagtatc
     1921 cgcgaaatat tcatccacat acaactcata tactccctct tgattacccg gcattatcct
     1981 cacatttttg atttccggtt ttagatgaat tctcgaagta atatcctgtt caaaatcatt
     2041 ccctagctcc cgcaatactt tgtattttga tttatcgctc ccatcgtcaa atatatcact
     2101 tattgaagcc ggaattgtta tttgcctttc aacctcagtg gactccgtat catctcccca
     2161 aactgcaact ttatattcca aagttttttg ctcgggtatt tgtcctgcaa acagctgcca
     2221 cgaaacctgc ctgtgactcc ctgctgccat atccccaacg gctatatcaa tatctgttcc
     2281 cgtatatggt actatttcat ccggcagaat gattttggcc ttcacatttt ttatagtatc
     2341 atccgtgtta ttggttatat atgccgtaat agtaaaaggg ttcggaacat aacccgtttc
     2401 ggttgcacta agactggtat tgccgcttac gataacaaca agcggtttat cactggcaat
     2461 ggtaaactta ctgataccat aatatgttac gtattccatt gtctcgcccg gtgccagttc
     2521 cttaggctcc caataagtcg caacggcact gtctccgttt gtgccggaat aattgaaatt
     2581 ccaaatgtta tcatataata cacgccacct tccaagcaca aatttatccg gcgggttttg
     2641 ctggtatttt acattggtgc cctgggaaac caccgtcggt tcaacaagac tgtccattgc
     2701 ctgccaataa tccggaattt tatcgcccaa aaatgtagtt gttgtcacta tatcccctat
     2761 gccgggaact ctgaaaggtg cactgtcatt gcttccaagc atcgtatcca gcatgattct
     2821 gattcccgca tcatggggaa cagtatcatt gtttgtaaca atgtacttga tttcaacagt
     2881 atcattaatg cccgaaaaac ccccatcaac caaacgtatg tcctgccgca cataaacatt
     2941 gccgtacttc gcaacggatg aattccggta ctcctcatca atattgtgcg gaggagttgc
     3001 aatattttct ccgccataat agtaattggt accgtcaagc ctgacggtgg tgtatgacgt
     3061 atcgccgccc tgccaaccat ataagagctt cttgccgtta tcggttgcaa tttccgggtt
     3121 gccttcattg gttccaattg taaaccttcc gtttggattt atagctacat taatatattc
     3181 attttccaaa tttatatgcc cagtatttga caaattattg ctattcatta tgccatctga
     3241 agattcgggt tgctccatag tattttcagc aaatacgtaa gaactgcaaa ttaaaaatac
     3301 taaaatcacc aacaaactcc acaaactata cttttttttc aacttcaata cccccttttc
     3361 tttctccgat taatacatat attcatccaa tgaagtaagc ttatatatga tttttgcgaa
     3421 ttctgccctg gtaatattca acttcggcct gaattcagga gtttcagaat ccaaggtata
     3481 aaaaccttcc cagccacggt tatacgctat ttttttcaca tcttttaatt gtccgccgga
     3541 aatatcccgt tcatcggaaa acattggcaa atcataatac ccttccggcg gttctttaaa
     3601 caagtccagg attttaacca acattgttgc cacatgctgt ctttgaacag gatcattcgg
     3661 accgaaactg ccgtcaccat atccgcttac atatcctaat ttcttagcaa cttttaccgc
     3721 attataatac cacgatcccg aagccacatc agtaaaagga tctccagact ctccaagggc
     3781 cggatgattg ttgtagtcaa ttccagccat atttaaagca attgtaatta tctgtgccct
     3841 tgtcacaact aaatcaggat caaaccgttt gttacctgtg ccttgcatat agcctttttt
     3901 aactacctct tttatgtatt cataatacca atccgatatt gaaacatccg taaaaagctg
     3961 agtttccggg tggtcaaaaa cagtaaccca ctctgtgaga ccgtcatgga tttgaatgtt
     4021 aaactctacc tgaaaaccgc attctctcag ccttcttcca agcccgttca ccggccttat
     4081 tacaaagttt gtatcgttta ttctttcaac aacacaaacc gggctgtctt catttccgta
     4141 attttgtatg cgggtatcga caacaggcaa ccttgtggtc aaggtatacg aatcttcgtc
     4201 cgcatcataa gttacttttt caatgtatgt ggaattctcg tacatatagc ttagtaattc
     4261 actggttttc ataacacaaa attccgttat gggatgcgac tcctcccacg gtatataagt
     4321 ggcggtgtag gtattaagtc cttcatttct tgtcaagaca attttacaat ccttccagtc
     4381 atcattgtct ttgctgtagg gagtattgca atcatatatg gacagtgtaa cggtattgag
     4441 ttcttcatcg gattcctgag catatgccag cagggcatgt cctcctacta aagcccatat
     4501 accgacataa agcacatccc cgcgtttcaa cgaattaaca atgtactcaa ctatttcttt
     4561 gggtatttta tcattgtcaa cactttttac tcgataaaac tcattcggaa ccattgtatt
     4621 agctataaca taccaacttt caatagcttt tctcacagca tgttcccttt catccgtgaa
     4681 tacaccccag atattggaat cccgccaatt gggattctgt gcgataatgg tatcctgtgt
     4741 tgagctcaaa ttatttgtag gttccagatc ataaatattg ttgccggttc gtccattacc
     4801 gaactcaaac accccgtcaa acaatctcag attataagct attttaggtg cttcgctatt
     4861 ttgtgtgggt aaatgatatg taaaaataca acttttat
//
LOCUS       DAOOJC010000148         4897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_13669_length_4897_cov_3.925651, whole genome shotgun sequence.
ACCESSION   DAOOJC010000148 DAOOJC010000000
VERSION     DAOOJC010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13669_length_4897_cov_3.925651"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1621
                     /locus_tag="PLA01_05250"
     CDS             <1..1621
                     /locus_tag="PLA01_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD00739.1"
                     /translation="SSLSRFEEALENYKKAFELEPDESLCANNIAYTFNCLEKYEDAL
                     KYSEKAIKLNKNNDSAYINKGYALEGLGKTSEALDCYNKSAEINPSNPICFYNKGNLL
                     ESLGKSEKAIDCFDKALELDPNDLNALNSKGDSLNSLGEFEKAIDCFEQAIEVDPSYV
                     DAYTNKIYCLYYQKKFSKCIEFSNQALDLFQDNQDIPWYIGDCYSALLDSDKAIEYYK
                     KALEINPESTSLIVSICWEYYSLQDYDKAREYLGKAKDISPNDEGVLYLESQLKKQDL
                     PEAERIVDFVKENYLYIDKVNNFEKLAEQFKAKNKVNIDDISQFIESVRLRDDMFTFF
                     IYGDEYDLMKQEETVSQVESKVLESNIHYIKIKSFTESVSWEFKDIIDRIESHEDKVL
                     VIDLRDNTGGVTSSSTDILNYLLPECTTSYIVYRNGSMYPYYSDKSFIKFKKILVLVN
                     EYSASSSEILTLGLKKYLNNVVIIGHTTVGKGVGQLVYEDKAKKYMIYLVSFYWNVME
                     ENIMEKRITPDVYVKGSDDSSYMKEIKRQAS"
     gene            complement(1997..3304)
                     /locus_tag="PLA01_05255"
     CDS             complement(1997..3304)
                     /locus_tag="PLA01_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD00740.1"
                     /translation="MLAIENSMELRDVDIIKEWNTLKEAIDKLPGIRPITVESWNRCK
                     RSNINPSNPQFVFLSKEELKQKLKENDFLIEAARPYMEFLSLSLTDIPHMVSLLDSES
                     WIIDIRGPVDELGGKDKGLCLDACRKEEYVGNNGGGTAISIKKPVFIYGVEHFSSIYK
                     SFSSLGVPIFRDSEVIGAINVSVLNEYAHPGRLTIAMLFVDAIEKELKKKARIRKNEE
                     PSKKMLSAAELMAIAVHDLKNPLASIRGLGQLGMMTSASKRERSYFERIIKQVDSLNN
                     SVVDLLSIFKPEKPVRVNPDLIITEIIEEMKPACDISNITLVLKTDNKSDINLHVKVF
                     RRAMENLIKNAAQALPDGGKIEIHINDKEDRILISIRDNGPGIPEQIKKSLFQPFVFR
                     RKDGTGLGLYMVQHAITEVHGGEIWFESELGKGTVFYISLPKE"
     gene            3571..4446
                     /locus_tag="PLA01_05260"
     CDS             3571..4446
                     /locus_tag="PLA01_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HPD00741.1"
                     /translation="MTKPVLNIKHAYKLKLVTLITAFVLFILCFSVFVHASQFIYGDI
                     DGSGSVDSTDLTLIKRYILRTITSFWYEKGAEAADVSGDGIIDSTDLTLIKRYLLRKI
                     DKFPAQTNQPTPSPTKPEPTPTATAKKGNVTYTLVTVSNPTEDQKDAYQRIKNAMDKA
                     VDYYNTYTTITKELRVYYEPSVSTADGNINGTIRFGSNRSYMNYITAMHEIAHTVGVG
                     TSSKWRSLMVNGVYTGSNAASVLREVTGDLNAVLKGDGSHFWPYGLNYTSEVKSEADL
                     INHCKIVDAMKKDGL"
BASE COUNT         1557 a         1039 c          846 g         1455 t
ORIGIN      
        1 ttcgagcctc agcaggttcg aagaggcact ggaaaactac aaaaaagcat ttgaactcga
       61 acctgatgaa agtctttgcg caaacaacat agcttatacc tttaattgcc ttgaaaaata
      121 cgaagatgcc cttaaataca gtgaaaaagc cattaaactt aacaaaaata atgattctgc
      181 atatatcaat aagggctatg ccctcgaagg cttggggaaa acttccgaag ccctcgattg
      241 ttataacaaa tcggcagaaa taaatccttc caatcccatt tgtttttata acaaaggaaa
      301 tttgctcgaa tccctcggaa agtccgaaaa agcaatagac tgctttgata aagcactgga
      361 attggatccc aatgatttga atgctctaaa ttcaaagggt gattccctga actcccttgg
      421 cgaattcgaa aaagcaatag attgctttga gcaggctatt gaagtagacc cttcctatgt
      481 tgatgcttat accaataaga tttattgtct gtattaccaa aaaaaattct cgaagtgcat
      541 tgagttttcc aaccaagctt tggatctttt tcaggataac caggacatac cttggtatat
      601 cggggattgc tactcggcac ttctcgactc cgataaagct attgaatact ataaaaaagc
      661 tctggaaata aatccagaat caacaagcct cattgtaagt atctgttggg aatactattc
      721 ccttcaggac tatgataagg ccagggaata tttgggcaag gcaaaagata tctctcccaa
      781 tgatgaaggc gtactttatc tggaatccca gcttaaaaag caagatctcc ccgaagctga
      841 aagaatagtg gactttgtta aagaaaacta cttgtatatt gataaagtaa acaactttga
      901 aaagctggca gaacaattta aagcaaagaa taaagttaat atagatgata tatcccaatt
      961 catcgaaagc gtaagactca gggacgacat gttcaccttc ttcatttatg gagatgagta
     1021 tgatttgatg aagcaagaag aaactgtttc tcaggttgaa tctaaagtac ttgaatcaaa
     1081 catacattac ataaaaatca agtcctttac cgaaagtgtg agttgggagt tcaaggacat
     1141 aattgacaga atcgagagcc acgaagacaa ggttctggta atcgatctta gagataacac
     1201 cgggggtgtt acttcctcat ccaccgatat tctgaattac ctgctgccgg aatgtaccac
     1261 cagttacata gtttatagga acggctccat gtatccttac tactcggaca agagcttcat
     1321 aaaattcaaa aaaatactgg ttcttgtaaa tgagtactcc gcaagcagct ccgaaatact
     1381 gacattaggt ctcaaaaagt acttaaacaa cgttgtcata atcggtcaca ccaccgtagg
     1441 taaaggagtt gggcaactgg tctatgaaga caaggcaaag aaatatatga tatacctcgt
     1501 aagtttctac tggaatgtca tggaggagaa cataatggaa aaaagaatta ctccggatgt
     1561 gtatgttaaa ggctccgatg attcttcgta catgaaggaa ataaaacgcc aggcttcata
     1621 aatagttttc cttcaaagat ccatgctttg gggacagcaa aagcgatggg ggcatcgatc
     1681 ccccatcgct ttttggtcat ttcacatcgt gaaattccac taataaaaaa agagatccaa
     1741 ttctacggac ctctctttta ccaaccttca taccaaacta tattacccgg cttaattatt
     1801 tgtacgcctt ttccacgtgc ccatattact ttgagcaagt ataataattt tcccactgtt
     1861 atagttattc gttaaacaat atcacaattg aggggtaaaa tacaaaaatt ttattttttt
     1921 cactctcaaa acatctttaa tatactatat acataggttt aacaatagtt tttaatgatt
     1981 cagaagaatt tttatctcat tctttgggca aagaaatata aaataccgtt ccttttccta
     2041 actcgctttc gaaccagatc tctcctccat gtacctcagt aattgcgtgc tgaaccatat
     2101 aaagccccag tcccgttcca tccttcctgc gaaatacaaa aggctgaaac aatgattttt
     2161 ttatttgttc cggtattccg gggccgttat ctcttataga tatgagaatc cggtcctctt
     2221 tatcgtttat gtgtatttct attttcccgc cgtccggtaa cgcctgggct gcgttcttga
     2281 tcaaattttc catagccctt ctaaacactt tgacatgaag gtttatatcc gatttattat
     2341 cagtttttaa aaccagtgta atattgctaa tatcacaggc aggcttcatt tcctctataa
     2401 tttccgtaat aatcaaatcg ggattaaccc ggacaggctt ttcgggctta aatatgctca
     2461 gcaaatcaac aacagaattg ttcaatgaat caacctgctt tattatcctt tcaaaatagc
     2521 tgcgttccct ttttgacgca gatgtcatca tacctagctg tcccaaccct cttatgctgg
     2581 ccagagggtt tttgaggtca tgtaccgcta ttgccatcag ctctgccgca gaaagcattt
     2641 ttttcgacgg ctcctcattt ttgcggattc tcgctttctt tttcagttct ttttctatag
     2701 catccacgaa taacattgct attgtcagcc gtcccggatg agcatactca ttcaacaccg
     2761 aaacattgat ggccccaatc acttcagaat ctctaaaaat cggcacccct aaggaggaaa
     2821 aagacttata tatgcttgaa aaatgctcga caccatatat gaaaacaggt ttttttatag
     2881 atattgccgt tcctccaccg ttattcccca catattcctc tttccggcat gcatctaaac
     2941 ataatccttt atctttcccg cccagttcgt caacaggccc ccgaatatct ataatccaac
     3001 tctcactgtc aagcaatgaa accatgtgag gtatatcggt caggctaagt gaaagaaatt
     3061 ccatatatgg ccgtgccgcc tcgattaaaa aatcattttc cttcaatttc tgcttcaact
     3121 cctcttttga caggaataca aactgtggat ttgaaggatt gatattcgat ctcttgcatc
     3181 gattccacga ctccactgtt ataggccgta tacctggcag cttatcaata gcctctttaa
     3241 gggtattcca ttccttaatt atgtctacat ctctcagttc catggaattt tcaatcgcca
     3301 gcatgttact gtactctccc tctcatagtt tttacacagc agttttttat tcgtcataga
     3361 atataaatat cctttatgtt taccgttatt tccgattatt tcccacaaaa aagtataaat
     3421 tcctttagat ttaacagcct tcagaccatg aaggcgtatt aaaaaagtat taaagaaata
     3481 tgaagtatgc aggagataaa taaaaatttt gcattttcgc cctcaaaaat actattatcc
     3541 acgtatatat atgacaagga ggcgaattgt atgaccaaac cggtattgaa tataaagcat
     3601 gcctacaagc tgaagcttgt gacactgatt actgctttcg tacttttcat cttgtgtttt
     3661 tcggtatttg tccatgcttc acagtttatt tatggagata ttgacggcag cggcagtgtg
     3721 gattctacag atcttacatt gattaagagg tatatattaa gaactattac ctccttctgg
     3781 tacgaaaagg gtgcagaagc cgccgatgtg agtggagacg gcataattga ttcaacagat
     3841 cttacattga taaaaagata ccttttacgg aaaatagata aatttcctgc ccagacaaat
     3901 cagcctacgc cttcaccaac caagccggaa ccgacaccca ctgctactgc taaaaaagga
     3961 aatgttacat acacactggt aacggtttca aatcccaccg aggatcaaaa agatgcatac
     4021 cagagaatca aaaatgctat ggacaaagca gtggattact acaacaccta taccacaatc
     4081 acgaaagaac tcagagtata ttatgagcct tcggtttcaa ccgctgacgg caacatcaac
     4141 ggcaccataa gattcggttc caaccgaagc tacatgaact atattacggc catgcatgaa
     4201 attgctcata cagtaggagt tggaacttcc agcaagtggc gaagcttgat ggtcaacggc
     4261 gtttacaccg gcagtaacgc tgcttctgta ctaagagagg ttacaggaga cctcaatgct
     4321 gtgcttaaag gtgacggttc tcatttctgg ccgtacggtc tcaattacac cagcgaggta
     4381 aaatctgaag ccgaccttat aaaccactgc aaaatagttg atgccatgaa aaaggacgga
     4441 ctataaattt attacttcct ctaaataatt ctatagcaaa gggcatgggt tatacgctcc
     4501 cgtgcccttt aaaaaaatta atggaacaaa aaagatctca cccgtgggta tcataaaatc
     4561 tttgtaataa aaaaaaatcg tctattaatc atgcctgttt tgcacaatta ataaactaca
     4621 aagctaattc tagttatatt ttactgtttg cggtgtgata aggcaataat aattaaagcg
     4681 tataaaatat cataaaagct atgctaaaaa tgtaaacatt tctctttttt attaaagttg
     4741 aaatctaccg aaccgtttat tatactccct caaaacttaa accttaatat attattgcct
     4801 tttctaccgt cgcaaatttc cctctcctac cattcgcaaa atctcaatgg tattgagccc
     4861 gttcgctctt tggccatttc acttagtgaa atccctc
//
LOCUS       DAOOJC010000149         4853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_13814_length_4853_cov_4.871613, whole genome shotgun sequence.
ACCESSION   DAOOJC010000149 DAOOJC010000000
VERSION     DAOOJC010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13814_length_4853_cov_4.871613"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /gene="yyaC"
                     /locus_tag="PLA01_05265"
     CDS             complement(<1..502)
                     /gene="yyaC"
                     /locus_tag="PLA01_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HPD00742.1"
                     /translation="MLVDTVCKQVYVDINSDWAFKKFTDALYTLISSSLKNGYDSIVF
                     ICIGTDRSTGDSLGPLIGYKLNDIEYKNVYVYGSLEEPVHAKNLDGVMERISSSYDRP
                     LVVAIDACLGRMDHVGYITLGQGSIKPGSGVNKDLAPVGDMHITGIVNFGGFMDYLIL
                     QNTRLSI"
     gene            complement(623..826)
                     /locus_tag="PLA01_05270"
     CDS             complement(623..826)
                     /locus_tag="PLA01_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00743.1"
                     /translation="MNDDILKNIVKTKLSLIDTFLGLLPGNIKSHVEDAKKSILEAAC
                     EYLQTGVEEKKDNEKGITKISIE"
     gene            complement(819..1991)
                     /locus_tag="PLA01_05275"
     CDS             complement(819..1991)
                     /locus_tag="PLA01_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HPD00744.1"
                     /translation="MRIILYTGKGGVGKTSIAAASAVKCAEKGLKTLVLSTDAAHSLG
                     DSLDIKLSCEPVKIQTNLWAQEIDSIHEVEHGWGKVQNYLTQLFTSKTIKDITTEELT
                     VFPGMEDLLSLLRILKYYKEEAYDVVIVDCAPTGETLALLSFPDMLRWWMDKLFPFKR
                     KALKLIKPIAEPILGLPMPSDNVMGEIENIYNQLDEMKQILSDRDVTSIRIVVNPEKM
                     VIKEAQRSFTYLNIYNYNVDAIVVNRVFPDVISDDYFKMWKDIQKKYKEMITDSFSPV
                     PIHNAPLFEQEIVGLDILRRLGNEVFGDEDPSRIYYNQRTQCVEKENNEYVLSIYMPF
                     TDKEDISLNQKADELIVRVGSIKRNITLPRTLLNYTVKGAKFDNEILKIRFGDSNE"
     gene            complement(2155..2637)
                     /locus_tag="PLA01_05280"
     CDS             complement(2155..2637)
                     /locus_tag="PLA01_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPD00745.1"
                     /translation="MDYTEILNYFIDNIKKLFYPEEWLNLDLSFSKSEIFVILVVDRQ
                     GEITMSKIADYINSPMSTATGIVDRLVKKGYLKRERSESDRRVVAIKLTDEGKSLIDE
                     FKKTAFKYIKLIYDSLTDEERVLVMKIFTRIVDIISSNESAEDSEDEDQNKLKKIDIE
                     "
     gene            complement(2776..3918)
                     /locus_tag="PLA01_05285"
     CDS             complement(2776..3918)
                     /locus_tag="PLA01_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HPD00746.1"
                     /translation="MKRYCFGIICIIVFASILAGIIYNFSSKQHVNLPAAASSIFLSE
                     NNKYGDKEQTLLGFIQNHLVGSFGEVATNTCPENGNTGTLSESIGILMYYSLINDDRP
                     MFERVYRYFEKELLTEGFHIKWRSGEDVTCNASIDDLRIIGVLFGAYEKWGDEKYFQV
                     SLTLQQKLYDFQVKGGSLYQLYDWKYNSTKASTPLCYLDLKTLKKLMYYNKDWEEVYD
                     RSLAIVSGGRIEGTPFYFKYYDYKSGEYQFDEEYNDNRGICLLYTLYTALNMAEEYFP
                     TDELLNWIKDEMDNRNKLYAWYDPYSKEPVSDLESTAVYALASIYARSSGDGVLSERL
                     LDRMLEFMVDDLNSKYYGGFGNCETGEFYSFDNLTALWALTLSGDK"
     gene            complement(4139..>4853)
                     /locus_tag="PLA01_05290"
     CDS             complement(4139..>4853)
                     /locus_tag="PLA01_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPD00747.1"
                     /translation="ELRYVIKPEYIVYPKGGVTVGEAADGNEKDEAKLVYAVKEGKPT
                     TFDANTGVILNYDGEPYKEQKPLVYTDIANHYAKSQIETLAENGIGLEGPELKPDEKI
                     KQKDFMLLLSQVVNDGYQFWGKSALRTDKETEDLYEILIREGIIKKDEINPEAPLTRE
                     DSVKFIIRALKYDKVADLSDIFNCSFKDKDEIDPALIGYVVIAKGLKIVNGSGDYFRP
                     KDELKKADALIIIYNYLGV"
BASE COUNT         1377 a         1129 c          744 g         1603 t
ORIGIN      
        1 ctatactcag gcgggtgttt tgtaaaatga gatagtccat aaacccgcca aaattaacaa
       61 tgcccgtaat atgcatatcg ccgacaggag caagatcttt atttacaccg gagcccggtt
      121 tgatggaacc ctgtccaagt gtaatatagc ccacgtgatc catcctgccg aggcatgcat
      181 caatcgccac aacgaggggt ctgtcatagc tgcttgagat tcgttccata accccatcca
      241 gattctttgc atgaacaggt tcctccaggc ttccataaac gtagacattt ttatattcaa
      301 tgtcattgag cttgtatccg attaagggac caaggctgtc tcccgttgac ctgtcagttc
      361 ctatgcaaat aaacactatc gaatcgtaac cgttcttcaa agaactgctt atcagtgtgt
      421 ataaagcatc cgtaaacttc ttgaatgccc aatcgctgtt tatatcaacg taaacctgct
      481 tacaaactgt atccacaagc atattcaatc tcccagctcc ataataactg caatataata
      541 ttattattgc cgtatacagt tatattatta ctctttttaa taattttgcc cgcaaaaaga
      601 gtacggagcc ggaattaaag ttctactcaa tggatatttt cgttatgccc ttttcattat
      661 ctttcttttc ctctactccg gtttgaaggt attcacacgc agcttcaagt atactttttt
      721 tcgcatcttc aacatgtgat tttatatttc cgggcaaaag acccaaaaat gtatctatca
      781 atgaaagctt ggttttaact atgtttttta aaatatcatc attcattaga atcaccgaac
      841 cttattttta atatctcgtt atcaaacttt gctcccttta cggtataatt taaaagcgtc
      901 ctcggtaacg tgatattcct ctttatgctt cccaccctca caatcaattc atctgccttc
      961 tgattaaggg atatatcttc tttatcagta aaaggcatgt aaatcgacag cacatattcg
     1021 ttgttttcct tttctacaca ctgtgtcctt tggttgtaat agatccggga tggatcctca
     1081 tcgccaaaca cctcattccc aagtcttctt aaaatgtcaa gaccgactat ttcctgttca
     1141 aacagaggtg cattgtgaat cggtacagga gaaaagctgt ctgttatcat ttccttatat
     1201 tttttctgaa tatctttcca cattttaaaa tagtcatcgg atataacatc cggaaatacc
     1261 ctgttgacaa caatagcatc cacattatag ttgtatatgt tcaggtaggt aaagctcctt
     1321 tgtgcttcct taataaccat cttttcagga tttacaacaa ttcttatgct tgttacatct
     1381 ctgtccgaca gtatttgctt catttcatca agttgattat aaatgttttc tatttcgccc
     1441 ataacattgt ccgacggcat cggaagtcca agaataggct ccgctatcgg cttaatcaac
     1501 ttcaatgctt tccttttgaa aggaaaaagc ttatccatcc accacctgag catatccgga
     1561 aagctcagaa gtgccaaagt ctcacccgtt ggtgcacagt caactataac aacatcatag
     1621 gcttcctctt tgtagtactt gagtattctc aagaggctaa gaaggtcttc catgcccgga
     1681 aatacggtga gttcctctgt agtaatatcc ttaatcgttt tggatgtaaa taattgtgta
     1741 aggtaattct gcactttgcc ccatccgtgt tccacctcat gtatagaatc gatttcctga
     1801 gcccaaagat ttgtctgaat tttcacaggt tcgcacgaca gcttgatgtc aagggaatcc
     1861 ccaaggctgt gagcggcatc tgtacttagc accaatgttt ttaagccttt ctcagcacat
     1921 ttcactgcac tggcagctgc gatacttgtc ttacccactc cgccttttcc tgtataaaga
     1981 attattctca taaaacccct cctgaaattt cgtttaataa atattacgaa tgtcgaaata
     2041 ttttctatat ataaatccaa acaccatcga aaatcctcaa tagtattcgg acaattctta
     2101 taaccaatac ttttttcaca cttgtatata aactaaggag tgcacatcag cagtttattc
     2161 aatatcaatt tttttaagct tgttttggtc ttcgtcctca ctgtcctctg ctgattcatt
     2221 gctgcttatt atatcaacaa tccgggtaaa tatcttcata accaagacac gttcttcatc
     2281 agtcaacgaa tcgtaaatca attttatata tttaaacgca gtttttttaa attcatcaat
     2341 cagcgacttc ccttcatcgg taagctttat tgccacaacc cttctatcgc tttcactgcg
     2401 ttcacgcttc aagtagcctt ttttaacaag ccggtccact attcctgtag cggtactcat
     2461 tggggaattt atataatccg caattttgct cattgtaatt tccccctgtc tgtctacaac
     2521 cagaattaca aagatttctg attttgaaaa agacaaatcc aggttgagcc actcttcggg
     2581 atagaataac tttttgatat tatcaataaa atagttaagt atttcagtgt aatccaaaat
     2641 ctatttcctc ctatgaatat ttcgatattc gtaatattct aatttaataa taatacattt
     2701 tcaatcaatt gtcaacggta tataagcaaa acatcccgat atgatgaata tatctgcatc
     2761 cataattatt tctcattact tatcaccgct taaagtcaat gcccataaag cggtaaggtt
     2821 gtcaaaagaa taaaattcac cggtttcaca attgccgaaa ccaccgtagt acttggaatt
     2881 taaatcatcc accataaact caagcatcct gtccaaaagc ctttcactca ataccccatc
     2941 ccctgacgac cgggcataaa tcgaagccaa tgcatagaca gcagtgctct ccaaatccga
     3001 gacaggctct ttgctgtacg gatcatacca ggcatatagc ttattcctgt tgtccatctc
     3061 atctttaatc cagttcaaaa gctcatcggt tggaaaatat tcttctgcca tgttaagtgc
     3121 ggtatacagc gtgtacaaaa ggcaaatccc ccgattatcg ttatactcct cgtcaaattg
     3181 gtattcaccg gacttgtaat cgtaatactt aaaataaaat ggagtacctt caatccttcc
     3241 tcctgacaca atagccaaac ttctgtcgta tacctcttcc caatccttgt tataatacat
     3301 taattttttc aatgtcttaa ggtccagata gcataaaggt gttgaagcct ttgtactgtt
     3361 atatttccaa tcgtacaact gatacaggct gccgcctttc acctgaaaat cgtatagctt
     3421 ttgctgcagg gtcaatgaca cctgaaaata cttttcatct ccccattttt catacgcccc
     3481 gaacagcaca ccgattattc tcaaatcatc tatcgaagca ttacaggtca catcttctcc
     3541 cgatctccat ttaatgtgaa agccttccgt aagaagctcc ttctcgaaat accggtacac
     3601 cctttcaaac atgggcctat cgtcatttat cagactgtaa tacattagaa ttcctatgga
     3661 ttccgacagg gtacccgtat tcccgttttc cggacaggtg ttggttgcaa cctcaccaaa
     3721 ggagccgacc agatggttct gtataaaccc cagaagtgtt tgttctttat ctccgtattt
     3781 gttgttttca gataaaaata tgctcgatgc cgctgcaggc aagttcacat gctgtttact
     3841 gctaaagtta taaataatgc ctgcaagaat actcgcaaaa actataatgc aaattatacc
     3901 aaaacagtac cttttcattt tttcgcccct tgtaaaaaat gtatgagtac ttatcctcaa
     3961 atattcttta tttccaacaa aggtgaagaa tactgctcta aaagcttatg ttttccacat
     4021 tatccttcac tttgcacctg ttgacggcga gttcatcaaa acaaaaaaat gggggcttga
     4081 tgcccccaaa gtttttttca ccaaaatctt tgaattgtat gtaaaaatgt taaagatact
     4141 acactccaag atagttgtaa attataatta atgcatctgc cttcttaagc tcgtctttgg
     4201 gtctgaagta gtctccgctg ccgtttacta ttttcagacc ttttgcaata acaacatacc
     4261 cgataagggc cggatctatc tcatccttgt ccttgaagct gcagttgaaa atgtcactta
     4321 aatccgcaac cttatcatac tttaacgctc ttataataaa cttcacactg tcttcccttg
     4381 tcaaaggtgc ttcaggattt atttcatcct ttttaattat tccttctctt ataagaattt
     4441 catataagtc ttccgtttct ttatcagtcc ttaaggcact cttaccccag aattgatatc
     4501 cgtcgtttac tacctgcgag agaagcagca tgaaatcctt ttgcttaatc ttttcatcgg
     4561 gtttcaattc cggaccttcc agtccgattc cgttttctgc caaagtctca atctgacttt
     4621 ttgcgtagtg gtttgcaata tctgtgtata ccaagggctt ttgttcttta tagggttctc
     4681 cgtcataatt caaaataact ccggtattgg catcaaaagt tgtaggctta ccttctttaa
     4741 cagcatatac aagctttgct tcgtcctttt cattgccgtc cgctgcttcg ccaaccgtaa
     4801 cccctccctt gggataaaca atatactccg gttttataac atatctaagc tcc
//
LOCUS       DAOOJC010000150         4832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_13887_length_4832_cov_3.437513, whole genome shotgun sequence.
ACCESSION   DAOOJC010000150 DAOOJC010000000
VERSION     DAOOJC010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13887_length_4832_cov_3.437513"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PLA01_05295"
     CDS             complement(<1..900)
                     /locus_tag="PLA01_05295"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPD00748.1"
                     /translation="MKIDGFKLFLSIVLLLLISSQIIYAAPVMAFSKNEAIYLIYKPI
                     ISFVENKFMYVFVFILLLFVVMHIYMGYLRKKISGMHSDLTKNNKWFKIILSSLGEVV
                     ITTDEKGIVTFSNLETQKLLGMYEDEIQGKELDVLFAKFCDSEGKVYRIPVEEVVKNG
                     LVVSLEGNIRFDNSDDKKLLAGTVAPIRNDSGTIIGTVIALKDITELKKKDEILYNME
                     YYDSLTGLPNKSLFSDRLNMALAQARRYKEMCAVIVLDLDNFKAINDTLGHSVGDELL
                     KQVAQKIKSILREVDTVARIGGDE"
     gene            complement(1352..2971)
                     /locus_tag="PLA01_05300"
     CDS             complement(1352..2971)
                     /locus_tag="PLA01_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPD00749.1"
                     /translation="MKKKGSIILLFAFLITFLYYNAPFSPLMPVSAADPNNDDWLHVE
                     GNRIVDMYGNEVWLTGCNWFGFNTGTNVFDGVWSCNMREAIQGMADRGINLLRIPIST
                     ELVHQWSRGVYPSANVNDFVNPELDGMNSLELFDFAFQCCKEVGIKIMVDIHSPQTDA
                     MGHNYPVWYDGSFTTEVWISSLEWLAERYKNDDTLLALDIKNEPHGKPGETLMAKWDG
                     STDVNNWKHAAETCAKRIMKINPNLLIVVEGVEVYPKPGYDYTAVDEWGKESKYYFNW
                     WGGNLRGVKDYPINLGEHQDQLVYSPHDYGPLVWRQSWFEKVFDASTLYNDCWRDNWA
                     YIHEGNIAPLLIGEWGGFMDGGDNEKWMNALRDFMIANRISHTFWCYNANSGDTGGLV
                     YYDFITWDEEKYALLKPALWQTADGKFIGLDHQIPLGSNGITVSEYYGGTIPTQRPTD
                     KPTPTPTPKPTEALESTPTETNPIEVLRGDVNVDNEVNSTDLTLLKRFVLKKIEEFPD
                     PLGYQAADVNDDGNIDSTDITMLKRILLRKI"
     gene            complement(3095..4798)
                     /locus_tag="PLA01_05305"
     CDS             complement(3095..4798)
                     /locus_tag="PLA01_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030248 - cellulose binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose binding domain-containing protein"
                     /protein_id="HPD00750.1"
                     /translation="MKGKKSIIVTETGLFEEINTREGMFIGQKIIYSQQDIIQNRRID
                     INYIYPVIASIAAVFVLLFSYFKLFYSDAPFAYVDIDINPSIEFEVDNKGIILREQPL
                     NQEAIEIMNGVTYEDKHLEDAIIDLIKKSKEYGYLKEDEGKDIILISAAIDIRKKIND
                     NDNRIINELVNNLRSDFIRLNQDIDIRFVEVSADLRKRAAENEVSMGKYLIYEKVLQE
                     GKYISLEMLAQTSLEELLNEYDVDLYGFTEPANTFQPSPSGNIYEATPKEEEKTTPTE
                     KKEPLPTPTEKIKLTPTPTEIIKSVPTPTKEILPTPKEKTEPTPTLTKEYEPTPTPTK
                     RYESTPTPTKEYEPTPTPTKKYEQTPTPTKRYEPTPTPTKKYETTPTPTKRYEPTPTP
                     TEKIKSTPTPTKISKPTPTQASGGKQGVKVQFYSNNEVSPATTIFLRIRVENTGDTAL
                     DLSDIKLRYYYTIDGYSDQVFTCDWSSVGAHNVTGRFVKISPSRSGADTFLEIGFGNN
                     SGMLEPGSSIDMNIRYTKSDNTEYNHEDDYSFKSTNYVYEDWDKITAYISGVLKWGRE
                     P"
BASE COUNT         1392 a         1072 c          758 g         1610 t
ORIGIN      
        1 ctcatccccg ccaatccttg caaccgtatc tacttctctt aaaatactct taatcttttg
       61 agctacctgc tttaaaagct catcaccgac agagtggcct aaagtatcat taattgcctt
      121 aaaattatcc agatcaagta ctattacagc acacatttcc ttatagcggc gtgcttgtgc
      181 caaagccata tttaaccggt ctgaaaacag gctcttgttt ggaagtcctg taagagaatc
      241 ataatactcc atattgtaaa gaatttcatc ctttttcttt agttcagtta tatccttaag
      301 tgcaataaca gtgcctataa tagttcccga gtcgtttcta attggggcta cagtacctgc
      361 cagcaacttt ttatcatccg aattgtcaaa cctgatattg ccttcaaggc ttaccaccaa
      421 tccatttttt acaacttcct caacgggaat cctgtacacc tttccctcag aatcacagaa
      481 tttcgcaaaa agcacgtcaa gttccttacc ttgaatctca tcttcataca ttcccaaaag
      541 tttctgtgtc tccagattgc taaatgtgac aattcccttt tcatccgttg ttattactac
      601 ttctcccaaa cttgacaata taatcttaaa ccatttatta ttctttgtaa gatcgctatg
      661 catgccggat atcttttttc ttaagtaccc catatagata tgcataacca caaaaagaag
      721 caatataaag acaaaaacat acataaactt attttccaca aaacttataa taggcttata
      781 tataagatat atagcttcat ttttagaaaa agccatcacc ggagctgcat atattatttg
      841 actgctaatc aacaaaagca atactataga taaaaacaac ttaaacccat cgattttcat
      901 cttattcctc cgattaagac tatgtgttca aagatcattc aaaaacaagt gataaatgct
      961 ataagaataa tatcggaaaa attaacacat taatctagac aagtcaattt acataagaac
     1021 acaagatact attatcatat attactatta tatataattt ttaatacact tttcaactcc
     1081 tatcagactt caaactatca tataactata gaaatagtgc tatggtccta atactaaaca
     1141 attaccaact ataattaatt aataccaact ttatgctctc caaaacatga aaaacaaaaa
     1201 tttcgtaaaa tgcaaagaat acttaacaat tgtgcccatc atcagtgagt acaacaaacc
     1261 gatgcatttt ctaaggtaac aaaaaactcc tccacaaaaa gcggagaagt tttattgtca
     1321 aattcaagtc aatcttaaca attactgtaa actaaatttt tctaagtagt attctcttaa
     1381 gcattgttat atctgttgag tcgatattgc catcatcgtt gacatctgcc gcctgatatc
     1441 ctaaggggtc gggaaattct tcaatcttct taagtacaaa cctctttaac aatgtcagat
     1501 ctgttgaatt tacttcattg tcaacgttta catctcccct taatacttct attggatttg
     1561 tttcagtcgg cgtagattcc aaagcttcag tcggtttcgg agtcggagtc ggcgtcggct
     1621 tgtccgtcgg cctttgagtc ggtatagtac ctccataata ttccgatacg gtaataccat
     1681 ttgaacctaa agggatctga tggtcgagtc ctataaactt tccatcggcc gtctgccata
     1741 atgccggctt taaaagagca tatttttctt catcccatgt tataaaatca taataaacca
     1801 gcccgccggt atcaccggaa ttggcattat aacaccagaa agtgtgggat atcctgttag
     1861 cgatcataaa atcacgcaat gcattcatcc acttttcatt gtctccgcca tccataaatc
     1921 caccccattc accgataaga agcggagcaa tatttccttc atgtatatat gcccagttat
     1981 ctctccagca atcattatac agggtacttg cgtcgaatac tttttcaaac cacgactgtc
     2041 tccaaaccaa tgggccgtaa tcatgaggcg aatataccag ttggtcctgg tgttcaccga
     2101 ggtttatggg ataatcctta acacctctta agttgccgcc ccaccagttg aaatagtatt
     2161 tgctctcttt accccattcg tctacggctg tataatcgta accgggtttc ggatatacct
     2221 caaccccttc taccacaatg agaagattgg gatttatctt cataatcctc tttgcacatg
     2281 tctctgcggc atgcttccag ttattaacat cagtagaacc gtcccacttg gccatgagtg
     2341 tttcaccggg cttaccatgg ggttcgtttt tgatgtccag tgccagaagg gtgtcatcat
     2401 ttttatacct ttcagccaac cactcaaggc tggaaatcca aacctcggtt gtgaatgatc
     2461 catcatacca aacaggataa ttatggccca ttgcgtccgt ttgaggacta tgaatgtcta
     2521 ccattatttt gattcccact tccttgcagc actggaacgc aaaatcaaac aattcaagac
     2581 tgttcatacc atcaagctcg ggattaacaa aatcatttac atttgcagaa ggatatactc
     2641 cccgtgacca ctgatgcaca agctcagtcg atataggtat tctcaataaa ttgatacccc
     2701 ggtctgccat gccctgaata gcctctctca tattacaact ccacactccg tcaaatacat
     2761 tggtaccggt attgaaacca aaccaattgc agccggttaa ccaaacttca ttgccgtaca
     2821 tatccactat cctattaccc tcgacatgaa gccaatcatc attattggga tctgcagcgg
     2881 aaaccggcat cagtggtgaa aaaggtgcgt tgtaatacaa aaatgtaatt agaaatgcaa
     2941 ataagagaat tatcgaacct tttttcttca ttaatattac ctccctaaaa acttcaaaaa
     3001 atctcacact atttgtattc tctctattct cctgaaaatc aaaaacccca ctatctcctc
     3061 cttcacaaga atttgacctg tatttttaag taaactaagg ctctcttccc catttaagaa
     3121 caccggatat atatgccgta atcttgtccc aatcctcgta aacataattc gtggatttga
     3181 acgaataatc atcttcatga ttatattccg tattatcaga ttttgtgtac cgtatattca
     3241 tgtctatgct gcttcccggt tcaagcatgc cggaattatt gccgaatcct atttcaagga
     3301 aagtatcggc acccgaacgg gacggactta tcttcacaaa cctgccggta acgttatgag
     3361 ccccgacaga agaccagtca caggtgaaaa cctgatcact gtatccatct atagtataat
     3421 aataccgcaa ctttatatcc gaaagatcca aagcagtatc cccggtattt tcaaccctaa
     3481 tcctcaagaa aatcgttgtg gctggagata cttcattgtt gctgtaaaat tgtactttaa
     3541 ctccctgctt cccaccggat gcttgagttg gtgtcggttt tgatatcttc gtcggtgttg
     3601 gtgtagattt tatcttttct gtcggtgttg gtgtaggttc gtatctcttc gtcggtgttg
     3661 gtgtcgtctc atatttcttt gtcggcgttg gcgtaggttc gtatctcttc gtcggtgttg
     3721 gtgtctgctc atatttcttt gtcggtgttg gcgtaggttc gtattccttt gtcggtgttg
     3781 gtgtcgattc gtatctcttt gttggtgttg gcgtaggttc gtattccttt gtcagtgttg
     3841 gtgtcggttc tgttttctcc tttggtgttg gcaaaatttc ttttgtcggt gttggcaccg
     3901 attttattat ctcagtaggt gtcggtgtta gctttatttt ttccgttgga gttggcaatg
     3961 gttccttttt ttccgttgga gttgtcttct cttcctcctt cggtgttgct tcataaatat
     4021 tgccggaagg tgaaggttgg aaagtattag caggttcagt aaatccatat aaatccacat
     4081 cgtattcgtt aagcaattct tctaaagaag tctgtgcaag catttcaaga gatatgtatt
     4141 ttccttcctg caagaccttt tcataaataa gatatttacc catagagact tcattttccg
     4201 cagctctttt tcttaagtcc gcagaaacct caacaaatct tatatctata tcctgattca
     4261 gcctaataaa atctgatctt aaattgttaa caagttcatt tataatccta ttatcattat
     4321 catttatttt tttacgaatg tcaattgccg ctgaaatcaa tataatatcc tttccttcat
     4381 cctcttttaa atagccatat tcctttgact tttttattaa atctataatt gcatcttcca
     4441 gatgcttatc ctcgtatgtt acaccgttca taatctctat tgcttcctga tttagcggtt
     4501 gctccctcag aataatacct ttattatcaa cctcaaattc tatactggga ttaatgtcaa
     4561 tatcaacata tgcaaaagga gcatcgctat agaaaagttt aaaatatgaa aacaaaagta
     4621 caaatacagc agctatgctt gcaattactg gatatatgta attgatatca attcttcgat
     4681 tttgtattat atcctgctga ctgtatataa tcttttgtcc tataaacatt ccttcccgtg
     4741 tatttatttc ctcaaacaaa cctgtttcag tcacaattat ggatttcttt cctttaattc
     4801 tggggttgct tctactgcaa ccaacagttt aa
//
LOCUS       DAOOJC010000151         4829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_13899_length_4829_cov_3.882698, whole genome shotgun sequence.
ACCESSION   DAOOJC010000151 DAOOJC010000000
VERSION     DAOOJC010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13899_length_4829_cov_3.882698"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLA01_05310"
     CDS             complement(<1..497)
                     /locus_tag="PLA01_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HPD00751.1"
                     /translation="MAMGFGSYEIARSGMFVNERALFVTGHNISNVNTPGFSRQQAMI
                     KSASYQDYGGFQLGLGADIQQLRQIRHQFLDNIYRDESTLLGYWETRNKTFQEIQTIL
                     GDPMEEGLQNVLNQFWDSWQELSKEPDSLTVRALVRQRAEALVYHINHVGQQLDKLQN
                     DLNYEV"
     gene            complement(517..1020)
                     /locus_tag="PLA01_05315"
     CDS             complement(517..1020)
                     /locus_tag="PLA01_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HPD00752.1"
                     /translation="MNNRLINDLIDVIEKELKIYEDLLEISKNKTDIIVKGKVKELES
                     ITELEQSLIVQIGKLESVREGLVDKICSDIGKDSSTNMTITELIGYAEAPQAERLNSC
                     KEKMTSALNEIKDINDINAKLIKSSIDFIDFSINIMSNINSDGNNYSDNGQVNDSEKK
                     TYFDVKL"
     gene            complement(1141..1434)
                     /gene="flgM"
                     /locus_tag="PLA01_05320"
     CDS             complement(1141..1434)
                     /gene="flgM"
                     /locus_tag="PLA01_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HPD00753.1"
                     /translation="MKVWDGIPKVGAVYDGPKSVNKTEKTSAAASKRDVVSISSQAKD
                     IQTAMKALKDIPDIRKDKVEPILKKFEAGNYEVKEEDIADKILKSIAENKSME"
     gene            complement(1625..2047)
                     /locus_tag="PLA01_05325"
     CDS             complement(1625..2047)
                     /locus_tag="PLA01_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPD00754.1"
                     /translation="MPDILNCKRCGKIYSYIGGAYLCNDCKRQDEEDFKRVKDYLYDH
                     PKASIYEVSKELDIGVERIKTYLRQGRLEIIGDDGNMILECELCGKSINTGRFCEKCA
                     SSLSKEIRSTVEQIKNPKTENKDVKSTGYMRYLHKDGK"
     gene            complement(2137..2802)
                     /locus_tag="PLA01_05330"
     CDS             complement(2137..2802)
                     /locus_tag="PLA01_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPD00755.1"
                     /translation="MLKRLRNLIFPPKCIFCNKIFDSNTDIYICKDCYSAIEFKEGGF
                     IKSGTGHDYFDSIVCVCEYTGIVKDAIKRYKFSNKPAYYRTFSGLLLSKIKEMTDYRL
                     FDTIISVPLHPIRERARGYNQSFLISMELSRQLGITEGSNLLSRTRNTHSQSLLQRKD
                     RLINVKGAFMVNDASKIKGKTVLLVDDVLTTGTTLNECSRVLKQAGAGKIIVAVVASG
                     RRF"
     gene            complement(2955..>4829)
                     /locus_tag="PLA01_05335"
     CDS             complement(2955..>4829)
                     /locus_tag="PLA01_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HPD00756.1"
                     /translation="AEIKGINPQKAIKIGQVFEEQRELRDVVLFFQEYGISPTYSAKI
                     YRAFGSDTIEQIRENPYRLSDEIFGISFKTADRIAKSLGIDPESKYRISSGIRYVLSQ
                     AAVDGHTFIDEEKLKEYTSQLLDVGIDNIDDVLISLVLKKSVYAEKDEGVPRVYLSSL
                     YNAELGVCRKLMELSNIKFNGNLDDFEERIREVQTKEGIILAHKQKEAIMEALINGVL
                     VITGGPGTGKTTIIKSIIALLEAEGYEFALAAPTGRAAKRMSEATGYEAKTIHRLLEI
                     GYTTDEDELVFARTEANPLEADVIIIDEMSMVDIVLMNHLLKAVPVGARLILVGDVDQ
                     LPSVGPGNVLRDIIKSEVIKTVKLSEIFRQAEESMIVVNAHKINRGEKPILNTRDKDF
                     FFVSRNSYSDTVRTIVDLCQRRIPDAYGFDPMKHIQVLTPMRKGPVGVGSLNIELQKV
                     LNPEDKNKKQKVFRDYIFREGDRVMQIKNNYNLKWEKPGDWRQSGTGVFNGDMGIIQE
                     IDDEDQRVVVCFDDERLVEYDFTILDELEPAFAVTIHKSQGSEFPVVILPVFPGPKVL
                     MTRNLLYTAITRAREMVILVGARDFLDEMIENDRETKRNSDLSGKLRKCILGIGTHHL
                     "
BASE COUNT         1240 a         1198 c          784 g         1607 t
ORIGIN      
        1 acctcatagt tcaagtcatt ttgcagcttg tccagctgct gtccgacatg gttaatatgg
       61 taaaccagag cttcggctct ttgtctcaca agggctctca ccgtaagact gtccggttcc
      121 ttcgaaagct cctgccatga gtcccaaaac tggtttaata cattttgcag tccttcttcc
      181 atgggatcgc caagaattgt ctgaatttcc tgaaaggttt tgtttcttgt ttcccagtat
      241 cccaacaggg tgctttcatc cctgtatata ttatccagaa actggtgccg aatctgtctt
      301 agttgttgga tatccgcccc aagtccaagc tgaaacccgc cataatcctg atatgaagct
      361 gatttaatca tcgcctgctg ccgggaaaag ccaggggtgt ttacattgga aatattatgc
      421 cccgtcacaa acaaggcccg ttcattcaca aacattcccg accttgcaat ttcgtagctg
      481 ccaaaaccca ttgccattta accgcctcca aatatcctac aatttaacat caaaatatgt
      541 cttcttttcg gaatcattta cctgtccgtt gtcgctgtag ttgtttccat cggaattaat
      601 attggacata atatttatcg aaaaatctat aaaatcaatt gagcttttta ttaatttcgc
      661 attgatgtca ttaatatcct taatttcatt aagtgcagaa gtcattttct ctttacatga
      721 gtttaatctt tcagcctggg gtgcctcagc atatccaatc aactcagtaa tcgtcatatt
      781 tgttgacgag tctttgccga tatcggagca tattttatca accagacctt ctcttacaga
      841 ttcgagtttt ccgatttgga caataagtga ctgttcgagc tctgtgatac tctccaattc
      901 ctttaccttc cctttaacaa taatatcggt tttgtttttg gagatctcca gtaagtcctc
      961 atatattttt aattcctttt caataacatc tattaaatca tttatcagcc ggttattcat
     1021 atattgctcc ctcactttta aggattaaat ttcactgctt tatttgcctg taataaattt
     1081 attgcgggaa aatttcggaa ttgtcctgaa aattaacccg caaatacact ttttataaaa
     1141 tcattccatg cttttatttt ctgctattga ctttaatatc ttatccgcta tatcctcctc
     1201 cttcacttca taattacctg cctcaaactt tttaagtatt ggttctacct tgtccttgcg
     1261 tatatccggt atgtccttta atgccttcat tgccgtctgg atatccttag cctgacttga
     1321 gatagacacc acatccctct ttgatgctgc tgcacttgtt ttttccgtct tgtttacact
     1381 tttgggtccg tcataaacag cacctacctt tgggattccg tcccaaactt tcataactac
     1441 cactcacctt ctttaaaaca tatatattaa cactttgcag tattaactta tcattggttt
     1501 ctcttaaaat ccttccctca ctaattatat cgtcatttaa ttaattaaat taacaaatat
     1561 ttatattttt ccttaaaact ttggctgcct acattttgcc ggtagacagc ccctgtttta
     1621 agtactactt tccgtcttta tgcaagtatc tcatatatcc ggttgatttc acgtccttat
     1681 tctcagtttt cggatttttt atttgttcga ctgttgatct tatttccttg ctgagtgaac
     1741 tggcacattt ctcacaaaaa cgtcctgtgt ttatggactt gccgcacaac tcgcactcaa
     1801 gaatcatgtt gccgtcatca ccaataattt caagccttcc ctgccttaaa taagtcttaa
     1861 ttctctccac acctatatcc agttccttag atacctcata aattgaggcc ttggggtgat
     1921 catacaaata atctttaacc ctcttgaaat cctcctcatc ctgtctttta caatcattgc
     1981 aaaggtacgc tcccccaata tagctgtaaa ttttaccgca acgcttacaa tttaatatat
     2041 ccggcatatt gatacctcct atcataacgc cagcatagca taactataat actccaacgt
     2101 ctggcaatac cataatattt attttcgagc acttcattaa aaccttctgc ctgatgcaac
     2161 tacggcgact ataatttttc cggcccctgc ctgcttcagc actctgctgc actcattcaa
     2221 tgtagtaccg gttgtcaaga cgtcgtctac caaaagaact gttttgcctt ttattttaga
     2281 agcgtcgttt accatgaatg caccttttac attaatcagt ctgtcctttc tttgcaacaa
     2341 gctctggctg tgtgtgtttc ttgttcttga caaaagattc gatccctctg ttatccccaa
     2401 ttgcctggat agctccatgg atatcaggaa agactggttg taaccccgtg ccctttctct
     2461 gattggatga aggggtacac ttataattgt atcaaacaag cggtaatcag tcatttcctt
     2521 tattttcgag agcagcagtc cggaaaaagt cctatagtat gccggtttgt tcgaaaactt
     2581 gtaccttttt attgcatcct tcacaatccc cgtatactcg cacacacaca caatgctgtc
     2641 aaaataatca tgcccggtgc cggattttat aaagccgcct tccttaaatt caattgccga
     2701 ataacagtcc ttacatatat atatatcggt atttgaatca aaaattttat tacaaaaaat
     2761 acattttgga ggaaatatca aattacgaag acgtttcaac atactccttc accaacttag
     2821 cctaattatt ataacaaggc agccagctat cttgtgcctc gtttaaacac tattgatgta
     2881 ataaaatttt actgcaaatg aaaaattatc ttcgatttga aatgttaaaa cgaggctata
     2941 tatgcccaca tcacttacaa gtgatgggta ccgattccca agatgcattt tctcagcttc
     3001 cccgacaggt ccgaattcct tttggtttcc cggtcatttt caatcatctc gtcaagaaaa
     3061 tcccgggcac cgacaagtat aaccatttcc cgagccctcg ttatagcggt gtataaaagg
     3121 tttctggtca tgagaacctt gggtccggga aatacgggaa gtatcaccac aggaaactca
     3181 cttccctggc tcttgtgaat agtaactgcg aaagccggct ccagctcgtc caaaattgta
     3241 aaatcgtatt ccacaagcct ttcatcgtca aagcatacca ccactctttg gtcctcatcg
     3301 tctatttcct gaattattcc catatcaccg ttgaatactc cggtgccgct ttgtctccag
     3361 tccccaggct tttcccattt gaggttgtaa ttatttttaa tttgcatcac ccggtcacct
     3421 tcacggaaga tgtaatccct gaaaaccttc tgtttcttgt tcttgtcctc gggattcaag
     3481 accttctgca gctctatatt gaggctgccg accccgaccg gtccctttcg catcggtgta
     3541 agcacctgaa tatgcttcat ggggtcaaat ccataagcat cgggtatcct tctttgacat
     3601 aagtccacaa ttgtcctgac ggtatcgcta taggagtttc gggatacaaa gaaaaaatcc
     3661 ttgtccctgg tgtttaatat aggtttttcc ccccggttaa ttttatgtgc atttacaacg
     3721 atcatgcttt cttcagcctg ccggaatatt tccgacagct tcaccgtctt tatgacttca
     3781 cttttaatta tgtcccgaag cacatttccc ggtccaaccg acggcaactg gtccacatcc
     3841 cccaccagta taagcctggc cccaacaggc actgccttaa gaaggtgatt cattagaact
     3901 atgtccacca tggacatctc atcaataatt attacatccg cctcaagggg atttgcctct
     3961 gtccgtgcaa acacaagctc atcttcatcc gttgtatacc cgatttccaa cagcctgtga
     4021 atagtctttg cttcatatcc tgttgcctcg gacattctct ttgcagccct gccggtgggt
     4081 gccgccagtg caaattcata tccctcggcc tcaagaaggg caatgatact ttttattatg
     4141 gtggtcttgc ccgtaccggg tccgccggtt atcacaagaa ctccgtttat caatgcttcc
     4201 atgatggctt ccttctgctt gtgtgcaagt ataatacctt cctttgtctg aacctccctg
     4261 attctttctt caaagtcatc cagattgccg ttgaacttga tatttgaaag ctccataagc
     4321 ttcctgcaaa cacccagctc cgcattatat aacgaactca aatataccct tggaacgcct
     4381 tcatcctttt ccgcataaac cgacttttta agcacaagag atatcaaaac atcatctata
     4441 ttatctatac cgacgtctaa tagctgggag gtatattcct taagcttttc ttcgtcaata
     4501 aaagtgtgtc cgtccactgc cgcctgggac agcacgtacc taatcccact ggatattcta
     4561 tatttggatt cggggtcaat tcccaagctt tttgcgattc tgtcggcggt tttaaagctt
     4621 atgccgaaaa tctcatcgga aagcctatag gggttctccc ttatctgttc aatggtatcc
     4681 gagccaaaag ccctgtaaat tttagcagag taggtgggac tgattccgta ttcctgaaag
     4741 aacagtacaa catcccgaag ttccctttgc tcttcaaaca cttgtcctat ttttatagcc
     4801 ttttgtgggt taataccctt tatttcggc
//
LOCUS       DAOOJC010000152         4790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14020_length_4790_cov_3.498838, whole genome shotgun sequence.
ACCESSION   DAOOJC010000152 DAOOJC010000000
VERSION     DAOOJC010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14020_length_4790_cov_3.498838"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..351)
                     /locus_tag="PLA01_05340"
     tRNA            complement(276..351)
                     /locus_tag="PLA01_05340"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(316..318),aa:Lys,seq:ttt)
     gene            complement(473..1744)
                     /locus_tag="PLA01_05345"
     CDS             complement(473..1744)
                     /locus_tag="PLA01_05345"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPD00757.1"
                     /translation="MATRVVIGTQWGDEGKGKYIDMLARTSDLVVRFSGGNNAGHTIV
                     VDGEKYALHLIPSGILHEGKTCIIGNGVVVDPAVLLEEIRDLNERGISTEKLLVSDRA
                     HVIMPYHRVLDELQEKFRGKDSIGTTKRGIGPCYCDKADRSGIRMCDLIDEEFFISKV
                     KDNLKVKNLIIEKVYGGQGLDEDQVISEYLEYGRQLKKYVADVNTIIFNAVEQNKEIL
                     FEGAQATLLDLDFGTYPFVTSSNPIAGGVCVGAGVGPVCINEVYGVLKAYTSRVGAGP
                     FPTEQDNEIGDKIRELGWEYGTTTGRPRRCGWLDTVMIRYAARVNGLTGLAINHVDTI
                     GKLPKIKLCVAYKKDGQETTSFPCSLKELAKCQPVYEEFDGWDMDISNVRTYNDLPEN
                     AKRYLNRIEELVGVKIKMIGVGKEREQTIVV"
     gene            complement(2079..2282)
                     /locus_tag="PLA01_05350"
     CDS             complement(2079..2282)
                     /locus_tag="PLA01_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HPD00758.1"
                     /translation="MGKITKDMIISDVLQMDRGTAPIFFNNGMHCLGCPSSSGESIED
                     ACAVHGLDADKLVKELNDYFDKK"
     gene            complement(2391..2951)
                     /locus_tag="PLA01_05355"
     CDS             complement(2391..2951)
                     /locus_tag="PLA01_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HPD00759.1"
                     /translation="MKYTLEAPFDDEKIKMLKAGDTVRINGIIYTARDAAHKKMISLI
                     NEGKSLPFDIRNQIIYYVGPCPARPGEVIGSAGPTTSSRMDAYAPKLIELGLKGMIGK
                     GLRSKAVVDAMKTYNAVYLGAIGGAGALIARSIVAQDIIAFPELGTEAIRKLTVKDFP
                     VVVIIDSNGDDLYRTGRDKYRALDQV"
     gene            complement(2981..3826)
                     /locus_tag="PLA01_05360"
     CDS             complement(2981..3826)
                     /locus_tag="PLA01_05360"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPD00760.1"
                     /translation="MRTVCVDDIIEAVERLCQEANYNLNDDIKNGLEKGARQEVSESG
                     KEILTHIIENADIARRERMAICQDTGMAVVFLDIGQDVHIAGGSLTDAINEGVRRGYD
                     KGYLRKSVVKDPVFERVNTMDNTPAVIHYNIVDGDRIKITVAPKGFGSENMSELKMLT
                     PSEGIDGVKRFIIETVDKAGPNPCPPVIVGVGIGGTMEKAALLAKKALLRPIDERSCE
                     PRIKELELELLESINRLGIGPAGLGGRVTALGVNVEVFPTHIAGLPVAVNISCHVTRH
                     SQTVI"
     gene            4105..4497
                     /locus_tag="PLA01_05365"
     CDS             4105..4497
                     /locus_tag="PLA01_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPD00761.1"
                     /translation="MSSPQNILVCVTQQITCERLILKAAEYRNELKGELFVIHVAKNE
                     WNFLDNIKEGEALEYLFKISKSVGANLSVLKSDNIVQTIVDFAKENKITHIVMGKSPN
                     DHKENNFYTELKSMLDDIEIHIIPQVET"
     gene            4689..>4790
                     /locus_tag="PLA01_05370"
     CDS             4689..>4790
                     /locus_tag="PLA01_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPD00762.1"
                     /translation="MNKTELVNSIASNSGLSKKNSEAALNALVSSVED"
BASE COUNT         1395 a         1137 c          725 g         1533 t
ORIGIN      
        1 aacccctgtt accgccgtga aagggcgatg tcttaaccac ttgaccaatg ggccatatta
       61 ttcaaagttt tatgtcagct aaccgacaaa gtatatttta actacatcca aataaaaagt
      121 caagcaaaat atttaaaatt ttaataagtt ttttttggtt ttttatgcac tcaattacta
      181 ccccctcaaa agtgggtata taaataattt cacttcactt aatcccacta aaaaaggcct
      241 cacacagcta tcgcttgcaa ggtctttcgc ctttttggtg agccatcggg gagtcgaacc
      301 ccggacacca tgattaaaag tcatgtgctc tgccaactga gctaatggct catattcaat
      361 tatgtgtatg catcacacaa ttatatatat tacaacagaa caaggtgctc tgtcaacaca
      421 atttttataa aaaaagactc aggaatcatg ttcttctccc tgagtcttgt tcttatacca
      481 ctatggtctg ttccctttcc ttacccacac cgatcatttt aatttttaca cctaccagct
      541 cttctattct gtttaaatat ctctttgcgt tctccggaag gtcattatac gtccgaacat
      601 tagatatatc catatcccat ccgtcaaact cctcataaac aggctggcat ttggcaagct
      661 ctttcagact gcaaggaaag cttgtcgtct cctgaccgtc cttcttatat gcaacacaaa
      721 gctttatttt gggtaatttc cctatcgtat cgacatggtt tattgccagt cctgtaagtc
      781 cgtttactct ggcagcataa cgtatcataa cggtatccag ccagccacag cgtctcggtc
      841 ttcctgttgt ggttccatac tcccagccaa gttctcttat tttatctcct atttcgttgt
      901 cttgttctgt cggaaagggg cctgcaccca ctctcgatgt ataagcctta agtaccccat
      961 atacctcatt gatacatacc ggtcccactc ctgcaccgac acaaactcca cctgcaatag
     1021 gattagagga tgttacaaag ggatatgttc caaaatcaag atccagtaat gtcgcctgtg
     1081 caccttcgaa caatatttct ttgttctgct ctactgcatt aaatataatg gtgtttacat
     1141 ccgctacgta ctttttaagc tgtcttccat actccaaata ttcggatata acctgatcct
     1201 catccagccc ttggcctcca tacacctttt caataataag gtttttgacc ttaaggttgt
     1261 ctttcacctt gctgataaaa aactcctcat caataaggtc acacattctg ataccggatc
     1321 tgtccgcttt atcacaatag cacgggccaa ttcctctctt tgtggttcca atcgaatcct
     1381 tgcctctgaa tttctcctga agctcatcta atactctatg gtatggcatt ataacatgtg
     1441 ccctgtcact cacaagtaac ttttcagtgc ttattccccg ctcattaagg tccctgatct
     1501 cctcaagtaa aacagcagga tcaactacta caccgtttcc aataatacat gtctttccct
     1561 catgtaaaat gcctgagggt ataagatgca gtgcatactt ttctccatcg actactatcg
     1621 tatgtccggc attattacca ccggaaaaac gcactactaa atcggaggtt ctggccaaca
     1681 tatcaatata tttgcctttg ccctcatcgc cccattgtgt accgattaca actctggtag
     1741 ccattctacc attccccctt tttcggaagt cagaccacaa acaattattt taatggtact
     1801 gcatatccac ttattatttc gaacctgaac aacataatta tgcgggttca ataaacattt
     1861 tccatgatat ataatatcaa aaaaaaacaa taaacacaat aaaaaatccg aacatttgca
     1921 ttaaaaaagc ataaaatagt tcggactttt tggtgatgca attcgtaaca tgaccaaaca
     1981 agcacctttg ccaatgtttc tggccaaatt atctttgcaa accagaaata aaacacattg
     2041 tttgcaaaaa accttcaccg tattaatcga taacccaact actttttatc aaaataatcg
     2101 tttagctctt taacaagctt atctgcatcc aacccatgga ccgcacaagc atcttctata
     2161 ctttctcccg atgatgacgg gcaacccaag caatgcattc cgttgttgaa aaatataggt
     2221 gcagtacctc tgtccatttg caatacatca gatataatca tgtctttcgt aatctttccc
     2281 atttctgtcc ctcctgatca ttatatattt taatcatttt actttgcgta catatattat
     2341 acactatcat cttataaaaa tatacacatt tctttcatat gaacatacag tcaaacctgg
     2401 tctaaagccc ggtatttatc ccttcccgtg cgatataagt catctccgtt gctatcaatt
     2461 attacaacga cagggaaatc cttgacggta agtttacgta ttgcctctgt tcccaattcg
     2521 ggaaaagcaa ttatatcctg tgcaactatt gaacgtgcaa ttaatgcccc tgctcctcca
     2581 atcgcaccaa ggtatactgc attgtacgtc ttcatcgcat ctacaacagc cttgcttctc
     2641 agccccttcc ctatcatacc ttttaatcca agctctatca gcttgggtgc ataagcatcc
     2701 attctgctgc tggtcgtagg tccggccgaa ccgattactt ctccgggtct ggccgggcaa
     2761 ggtcccacat aataaataat ctgatttctt atatcaaaag ggagtgattt cccttcgttt
     2821 ataaggctta tcatcttctt atgtgctgca tccctggctg tatatataat cccgtttatt
     2881 ctcacggtat cccctgcttt gagcattttt atcttttcgt catcaaaagg agcctccaat
     2941 gtatatttca tatttaccac ccttcttaaa taagtaacaa ttaaataacg gtttgtgaat
     3001 gtctcgtcac atgacagctt atatttacag caactggtag gcctgcaatg tgcgtcggaa
     3061 aaacctcaac attgacgcca agggctgtaa ccctgcctcc aagaccggcc ggtcctatcc
     3121 ctaacctgtt tatactttcg agcaattcca attccaattc ttttatacgg ggttcacagc
     3181 ttctttcatc aataggcctc agcaaagcct ttttggctaa caaagccgct ttttccatag
     3241 ttccccctat gccaacaccg acaattacgg gaggacaagg gttggggccc gctttatcaa
     3301 ctgtctcgat tataaacctc ttgactccgt caatcccctc tgaaggcgtg agcatcttaa
     3361 gctcactcat gttttcactg ccgaatccct ttggggccac tgtgattttt attctgtctc
     3421 cgtcaactat attataatga atcacagccg gtgtattgtc catagtgtta accctttcaa
     3481 aaacagggtc ctttacaacg gattttctga gatatccttt atcatagcct cttcttaccc
     3541 cttcattgat tgcatccgta aggcttcctc cggcaatatg cacatcctgc ccgatatcca
     3601 agaaaaccac cgccatacct gtgtcctggc aaattgccat gcgttctcta cgggcaatat
     3661 cagcattttc tataatatgg gtcaaaatct cttttccact ttcagatacc tcttgccggg
     3721 cccctttttc aagcccattt tttatgtcat catttaaatt gtaatttgct tcttgacaaa
     3781 gccgttccac ggcttcaata atatcatcaa cacaaacagt tctcataaca ccctccagat
     3841 aaatttaacc accatgcctt ctgttctctc taatcaagac aacattgctt tctttttaat
     3901 tctagtatac cgctgtgtat ttttcaagaa agattaattt ttattattta aatttatcca
     3961 taggactttt ttacgtaact ttggtataat aaatatatgg ctacgtaaca atagcctttt
     4021 ttggcaatgt tatgcgtttt attgctcttt gaaaattctc agcaccatta attctcgtga
     4081 cttcagagga aaggggtttt tattttgagt tctcctcaga acattttggt ttgtgtaaca
     4141 cagcaaatta cctgtgaaag attaatacta aaagcagcgg aatataggaa cgagttaaaa
     4201 ggcgaactct ttgtaataca cgttgcaaaa aacgaatgga actttttaga caacataaaa
     4261 gaaggtgaag ccctggaata tttatttaag atatcaaagt cggtaggtgc caatctatcg
     4321 gtattaaagt ccgataacat agtacaaact attgttgatt ttgcaaaaga gaataaaata
     4381 acacatattg ttatgggaaa atcccccaac gaccacaaag aaaataattt ttatactgaa
     4441 cttaaaagta tgctggatga tatagaaatc catatcatac ctcaggtcga aacatgacgt
     4501 atataaccaa atcagcttat ttcaaactaa tggccaaata ttatacacct aaaaatattt
     4561 ttatgcaagc aaaattcatt tttttaaaaa aaatttgctt aaaaccacta aattgttcgt
     4621 aattacttga ctttacacga ttttcattat aaaataatgt cgaattccat attaaggagg
     4681 ttgtgtaaat gaataaaact gaattagtaa attccattgc ttccaactca ggacttagca
     4741 aaaagaacag cgaagctgct cttaatgcac tggtttcttc tgtagaagac
//
LOCUS       DAOOJC010000153         4782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14055_length_4782_cov_3.272900, whole genome shotgun sequence.
ACCESSION   DAOOJC010000153 DAOOJC010000000
VERSION     DAOOJC010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14055_length_4782_cov_3.272900"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PLA01_05375"
     CDS             <1..589
                     /locus_tag="PLA01_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HPD00763.1"
                     /translation="SKGVGVYFVTQDPCDLPDEVLGQLGNRIQHALRAFTPKHQKMVK
                     AAAQTFRTNPDVDIETAITELATGEALVSCLDEHGSPGIVERAFILPPQSQMGTIDDT
                     TRKDIISSSLIYGKYDKMVDNESAYEVLQAKGRQAAKVEEQKSAKWQSSVKTSQKRTS
                     NKKSILEKAFGSAATSIGRELGRSLIRGILGSLKK"
     gene            783..1616
                     /locus_tag="PLA01_05380"
     CDS             783..1616
                     /locus_tag="PLA01_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HPD00764.1"
                     /translation="MNRKRRYKLVAVMLILFCIGLITSYDVGAVTAKAYITVSVNDKC
                     IKMDSLPYLKDDRTFVSIRFVAQALGAEIGWVDEKKMVVINDGYNYIELFIGSNEYYV
                     NSEKRTMDVAPEISNGRTMVPLRFVSENLRCSVDWNEMTYTVLINKEGLEVPEEYVYN
                     RSYTDEDLMWLARIVTVEARGATIEGKLAVANVVLNRVKSPAFPNTVHDVIFQSGQFP
                     PAHKAGFKELVPPKDCIIAAKMALEGENNIGTCLYFNNRPFTSRSGSFYKKIDGEYFY
                     N"
     gene            complement(1740..3851)
                     /locus_tag="PLA01_05385"
     CDS             complement(1740..3851)
                     /locus_tag="PLA01_05385"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPD00765.1"
                     /translation="MKHADKKELILEGLNCASCAAKIETKVKEIDGVKNASLNFVTKK
                     LTLEFNNVLMSEDIVKKASMIAKSVESDIEVIEQEGKKTEQDSGFNKSEVIALSIGIL
                     LYVTAFIISFPFWIEFFLYLTSYLLIGQDVLVKAVRNIFKGKLMDENFLMTIATVGAF
                     SIREFSEGASVMLFYKVGELLQDMAVNRSRKSITDLMNIRPDYANLKSGENIRKVSPE
                     EVNIGNIIVVKPGERIPLDGRIIEGRSTVDVSALTGESVPKDVETGDEVLSGTVNKSG
                     LLSIEVTKKYADSTVSKILDLVQNASSQKAPTENFITKFARVYTPIVVFTSAALAIIP
                     PLVVPGASFSEWLYRSLIFLVISCPCALVISIPLGYFGGIGAASRHGILIKGSNYLEA
                     LNSVNTIVFDKTGTLTKGVFEVTELNPSNGFSKESLLEYAAIAERFSNHPIALSITKA
                     FGKGLDFYETENFEEIPGQGIRTMYQGKEILAGNEMLMNNSGIKISDTNGSGTTIHVA
                     VDGKYAGNISISDEIKTDSKQALDELKKIGVQKLIMLTGDNDNQARKIGRQLGFDEVY
                     AELLPHQKVEKIEIIAGQKALKDKLAFVGDGINDAPVLARSDIGVAMGGLGSDAAIEA
                     ADIVLMTDEPSKLVSAIKIARKTKGIIWQNIIFAFAVKIIVLSLGARGMATMWEAVFA
                     DVGVAVLAVLNAMRLIRVKNL"
     gene            complement(3875..4240)
                     /locus_tag="PLA01_05390"
     CDS             complement(3875..4240)
                     /locus_tag="PLA01_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPD00766.1"
                     /translation="MHENYGSDKCDCNIIHEDVVNMVRENMPKEEHLYDLAELFKVFG
                     DTTRIKILYALFASEMCVCDIASLINMTQSAVSHQLRVLKQARLVKYRKDGKVVYYSL
                     DDEHIKQIFDQGLMHITEN"
     gene            4602..>4782
                     /locus_tag="PLA01_05395"
     CDS             4602..>4782
                     /locus_tag="PLA01_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPD00767.1"
                     /translation="MRKVVIFFLAFCIIVGLCANPSFSAGTYSTKYGDVNGDDLIDST
                     DLTILKRYLLKKLSEF"
BASE COUNT         1412 a          903 c          951 g         1516 t
ORIGIN      
        1 ttcaaaaggg gtcggagtat attttgttac tcaggatcct tgcgatttgc cggatgaagt
       61 actcggtcag ttgggaaacc gtattcagca tgctcttcgt gcctttacac caaagcatca
      121 gaaaatggtt aaagctgcag cacagacttt ccgtacaaat cctgatgtgg atattgagac
      181 agctattact gagcttgcta ccggagaagc cttggtgtca tgtcttgatg agcacggaag
      241 ccccggtata gtggagcggg catttattct gccgcctcaa agtcagatgg gtactattga
      301 tgatacaacc cggaaggata ttatttcatc ttcacttatt tatggaaagt acgataaaat
      361 ggtggataat gagtctgcct acgaagtgct ccaggctaaa ggaagacagg cggcaaaggt
      421 tgaagaacag aaaagtgcaa agtggcaaag cagtgttaaa acatcacaaa aacgtacttc
      481 caacaaaaaa tctatcttgg aaaaggcatt tggttctgct gcaacatcaa tcggaagaga
      541 gcttggaaga agcttgatac gtggcattct cggttctctt aaaaagtgat gccgtttcgg
      601 tcaaggtatt ttactatatg gaaaaaatta catatggata tatatgggta tatgcaatta
      661 tgacttacaa ttgaatttat tttatgataa aattgtactg gtgttatgtg gcagtgtcgg
      721 ttgtgagaaa tgaggctgct gcgtatgcca tataaattta agtattggat ggtgtttgtc
      781 atatgaatag gaagagaagg tataagcttg tagcagttat gcttatactg ttttgtatcg
      841 gcttaatcac tagttatgat gtaggagccg taactgcaaa agcttacata actgtatcag
      901 taaacgacaa atgcataaaa atggacagct tgccatatct taaagacgac agaacctttg
      961 tttcgatacg ttttgttgct caagccttag gagctgagat aggatgggta gacgaaaaaa
     1021 agatggttgt aataaatgat ggctataatt atatcgaact ctttataggt agcaatgagt
     1081 actatgtaaa ttcagagaag cggacaatgg atgttgcccc ggaaataagc aatgggcgga
     1141 caatggttcc gttgaggttt gtttctgaaa acctaagatg ctctgtggac tggaatgaga
     1201 tgacctatac ggtattgata aacaaagaag gtctggaggt tcctgaggaa tatgtttaca
     1261 ataggtctta taccgatgaa gatttgatgt ggcttgcaag aatagttacc gttgaagcca
     1321 ggggagcgac tatagaagga aagcttgctg ttgcaaatgt tgtattaaac agggttaaaa
     1381 gtcctgcgtt tcccaacacg gtgcatgatg taattttcca gagcggacaa tttccacctg
     1441 cccacaaggc tgggtttaag gaattggtac ctccgaagga ttgcattatt gcagcaaaaa
     1501 tggctttaga gggtgaaaat aatattggaa catgccttta ctttaataac aggccgttca
     1561 catcaagaag tggtagtttt tacaaaaaaa tagacggcga gtatttttac aattgagtca
     1621 gtttgaataa aaagaaatgt taagagaaga aaaatgctgt agagacttag gtttcttcag
     1681 catttttctt tactcagaca atatatgtct aatgatatta ttcgaaatat attttacaat
     1741 taaagattct tgaccctgat tagtctcatg gcatttaata ctgccaatac tgcaactcca
     1801 acatctgcaa aaactgcttc ccacatcgta gccatacctc tggctccaag ggacaggact
     1861 attattttta cggcaaaggc aaaaataatg ttctgccaga ttataccttt ggtttttcgg
     1921 gcaattttga tagcactgac aagctttgaa ggctcatcgg tcatcaacac aatatctgct
     1981 gcttcaatgg ctgcatccga tcccaaacct cccattgcca ctccaatatc cgatcttgcc
     2041 aataccggtg cgtcatttat tccgtctcct acgaaggcaa gcttatcttt aagagctttt
     2101 tgaccggcta taatttcgat cttttccacc ttctgatggg gaagaagttc agcatatacc
     2161 tcatcaaaac ccaattgccg gccgattttt cgggcttgat tatcattatc tcctgtaagc
     2221 attatcaact tttgtacacc tatctttttc agttcgtcta atgcctgctt cgagtcagtc
     2281 ttaatttcat cggatatcga tatattacct gcatattttc catccacagc tacatgaatt
     2341 gttgtgccgg agccattagt atctgagatt ttgatgccgg aattattcat cagcatttca
     2401 ttccctgcca gtatttcttt gccctgatac atggtcctta ttccctgccc cggtatttct
     2461 tcgaagtttt ccgtttcata gaagtccaaa ccttttccga aagccttggt tatagatagt
     2521 gcaataggat gattggaaaa tctctctgct attgcagcgt attccagcag gctttctttt
     2581 gaaaacccat tcgatgggtt caattcagtt acctcaaata caccctttgt aagcgtgcct
     2641 gttttgtcga acactatagt attcacacta ttcaaagctt caaggtaatt gctgcccttt
     2701 atgagtattc catgccttga agccgctccg attcctccaa aatacccgag gggtatagag
     2761 ataacgagag cacatggaca cgaaatcacg agaaatataa gggacctgta gagccattcg
     2821 gaaaaagatg caccgggaac cacaaggggc ggaattatgg ctaaagctgc tgaagtaaat
     2881 accacaatgg gggtataaac ccgtgcaaat ttagtgataa agttttctgt aggagccttt
     2941 tgactgcttg cattttgtac aagatccaat atttttgaca cggttgagtc ggcatatttt
     3001 tttgtgacct caatactcaa aagtccgctt ttgtttaccg tacccgacag aacttcatcc
     3061 cctgtctcca catccttcgg tacggactcg ccggttaatg cggatacatc aacagtggat
     3121 cttccttcaa taatccggcc gtccaaaggt attctttctc ccggcttgac cacaataata
     3181 ttaccaatat tgacttcttc gggagatact ttcctgatgt tttctccgga tttaagattg
     3241 gcgtagtcag ggcgtatatt cataagatcc gtaatggact ttcttgatcg gtttacggcc
     3301 atatcctgga gaagttcgcc aaccttataa aaaagcataa cggatgcacc ctcagagaat
     3361 tccctaatgg aaaacgcacc gaccgtagca atggtcatta aaaagttttc gtccataagc
     3421 ttacctttaa aaatattacg cacagccttt accaatacat cctggccaat caataaataa
     3481 ctggtaagat acaggaagaa ttcgatccaa aagggaaaag atattataaa tgcggtgaca
     3541 tacaacaaga tgccgatgct aagagctatg acttctgatt tattgaatcc gctgtcttgt
     3601 tctgtttttt tcccttcctg ctcaattact tcaatatccg attctacact cttggctatc
     3661 attgatgctt tcttcactat gtcttctgac attagcacgt tgttaaactc taaagtaagc
     3721 ttttttgtta caaaatttaa agatgcattt tttactccat ctatttcttt aaccttggtt
     3781 tcgatttttg ctgcacagct tgcacaattc aggccttcga gtattagttc ttttttatct
     3841 gcatgtttca ttttaaaccc ctgccttgtt aaattcaatt ttctgttata tgcatgagtc
     3901 cctgatcgaa tatctgttta atatgctcat catcaagaga gtagtatacg actttaccgt
     3961 cttttctata ctttacaagc cttgcctgtt ttagaaccct gagttggtgt gataccgccg
     4021 attgtgtcat gttgataagg gatgcaatat cacaaacgca catttctgag gcaaacaatg
     4081 cataaagtat ttttattctg gtggtgtcgc caaagacctt gaaaagctct gcaagatcgt
     4141 aaaggtgttc ctccttgggc atattttcac gcaccatgtt taccacatct tcatgaatta
     4201 tgttacagtc acatttatcg gatccgtaat tctcatgcat attaattcct cctcatttat
     4261 ctgaattcta gaactctacc tgttattagg tggagttggt attaaataat gcaaatgaat
     4321 tattgaacat catatgcaca tatgaataat tgttcatata ttacaatgtc attatacgcc
     4381 tattgtattg aagatgtcaa taattttttt tgagtaagag tattatttta tttatgtgaa
     4441 taaaaacatg gaagaaacat aggttactgg aatgacttta tttacactta ttcagaattt
     4501 ttatgacaga aaaaattatt tttttttata cccctaaaaa aagaatagaa gacgtatttt
     4561 aaagtaaaag gtataataga attaagggag gaattaatgg tatgagaaag gtagtgattt
     4621 tttttctggc tttttgcatt attgttggat tatgtgccaa tccaagcttt tcagccggca
     4681 catattcaac aaaatatggg gatgtgaatg gggatgactt aattgattcg acggatctaa
     4741 ccatattaaa gaggtatttg ctgaaaaagt tatctgagtt tc
//
LOCUS       DAOOJC010000154         4731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14230_length_4731_cov_3.471343, whole genome shotgun sequence.
ACCESSION   DAOOJC010000154 DAOOJC010000000
VERSION     DAOOJC010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14230_length_4731_cov_3.471343"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /gene="pgeF"
                     /locus_tag="PLA01_05400"
     CDS             <1..709
                     /gene="pgeF"
                     /locus_tag="PLA01_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HPD00768.1"
                     /translation="GVSKSPYESLNMAFNKRDERENVEENYRRVAKALGIDIWDMVFS
                     NQVHDNRIRVVTEEDRGKGIIKHSDIIGFDGLATNKRKVALVTFYADCVPVFLFDPVK
                     TVISIAHSGWRGTVKEIARVAVRKMKDAFSCDPKDILAAIGPSIGSCCFEVGDEVYEE
                     FGSSIGWSTKYCERKNGKWYFDLPAIIKNSLSNEGVKGENILLSGICTKCNKDMFFSH
                     RGDNGKTGTLAGIMMIN"
     gene            848..2521
                     /locus_tag="PLA01_05405"
     CDS             848..2521
                     /locus_tag="PLA01_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HPD00769.1"
                     /translation="MKKRTLSVVLFILVSLIISGCSVDLNSLLGDNTDNDYSFLNNDA
                     DDILDTGPVKSGILNLFATAPDTLNPILTANAFVKDYSCFVYESLVKLNEKQKPVPVL
                     AERWESSEGGKVWTFYLKDNIYWHDNIRFSAEDVEFTAGTIVNAGANSPYAASFKNVV
                     SFSAVDSKTFKVEMDSPNSFTPELMTFPIIPKHYYLGEDIMNTEKNNRPIGTGPYKFA
                     EYKENEFVKLQSNENWWNNTDTDEDGLTLPYIQEINIKIFGKDSSVINAFQSKEVDVI
                     IADTKNWIGYSGRSDITLKKYLSNRFEFIAFNLSNKILKEKEVRTAIAYTVDRGKLVN
                     DIMPGEAVVSDLPVIPDTWLHDTNVISYERDIEKAKQILEDSGWKEKNGVLYKRINGV
                     NTPLTLELLVNTDNERRLEVAEEIKDQLKEAGIVLNIKSVTWEDELNKVKSGKFDMAF
                     IGCTVPSIPDISFLYSSAEIGTGINIAGYENESVDNYLSQILRETDSSRKKAYFFNMK
                     EAINEDMPYLGLYFFYDAAIYNKRIRGEFSPCIWDKYDNFTKWYIPDVE"
     gene            2625..3131
                     /gene="lspA"
                     /locus_tag="PLA01_05410"
     CDS             2625..3131
                     /gene="lspA"
                     /locus_tag="PLA01_05410"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPD00770.1"
                     /translation="MVWIIIIVLILALDQITKFIVSSNFELGDSTTIIDNFFHIVHWR
                     NTGAAWGIMQNGKYVLVPVTFLLVLLMAYFMYKYNNKLLRVALSMVIGGALGNLIDRV
                     FRSGGVVDFLDFQFGSYHFPSFNIADSFVVVGTIIMAYYLFFIHKDKEESSSKKPVDE
                     KTTEQREN"
     gene            3209..4123
                     /locus_tag="PLA01_05415"
     CDS             3209..4123
                     /locus_tag="PLA01_05415"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPD00771.1"
                     /translation="MKEIKLVCEDKGTRVDLWLSERLEEYSRSYIKKMIDDEMLLVNG
                     EHVKSNYKLKSGDEVLLRIPDPKPLDVCAEDIEVPILYEDEDIVVVDKPRGMVVHPAA
                     GNYSGTLVNALIGYCGDNLSDINGVIRPGIVHRIDRDTSGVLVVAKTNTAHERLSEKL
                     KVHDIERVYVALVYGVIAENQGKIDAPIGRHPTDRKKMAVNAKNGRSAVTHFRVLERF
                     KDATYIEVTLETGRTHQIRVHMSYIGYPIIGDTVYGRKNDRYDINGQALHAKELGFIH
                     PTREKYMEFESPLPEYFENLLEELRQNR"
     gene            4381..>4731
                     /locus_tag="PLA01_05420"
     CDS             4381..>4731
                     /locus_tag="PLA01_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00772.1"
                     /translation="MKEIEQAYLYAVSKRKKFVIILFACAICIAILALLEKIKESDMF
                     SIVVISCITIFLILLGILVINSPNIKGSFRSSRVYKSLENLGYADEFINTIDYEIQNQ
                     LRINYHDDVYHIGLF"
BASE COUNT         1547 a          705 c         1096 g         1383 t
ORIGIN      
        1 aggggtaagc aaaagcccct atgagtcgtt gaacatggca ttcaacaaga gggatgaaag
       61 agaaaatgtg gaagaaaact atagaagagt ggcaaaggca ctgggtattg acatttggga
      121 tatggttttt tcaaatcagg tacatgacaa cagaattcgg gtggtaacgg aggaggacag
      181 aggcaaaggt attataaaac atagtgatat aataggcttt gatgggcttg ctacaaataa
      241 aagaaaggtg gcactggtta ccttttatgc cgactgtgtg cctgtttttc tgttcgatcc
      301 ggtcaaaacc gtaatctcaa ttgctcattc tggctggcgt ggtacagtga aggaaatagc
      361 aagagtagct gtcaggaaaa tgaaggatgc tttttcctgc gatccgaagg atatattggc
      421 tgcaataggg ccgtcaatag gcagttgctg ttttgaggtg ggggatgagg tatatgaaga
      481 gtttggatca agcatcggct ggagcacaaa atactgtgaa agaaagaatg ggaaatggta
      541 ttttgatctt cctgcgataa taaaaaacag tctttcgaat gaaggagtca aaggtgagaa
      601 cattctttta agcggcatat gtaccaaatg caacaaagac atgttttttt ctcaccgtgg
      661 cgataacggc aaaacaggaa ctcttgcagg tattatgatg attaattgaa tattgccttg
      721 gcaccggaag aattctgccc agggctgtta attgagggaa ttgttaaagg cagttgctat
      781 aatacaaagg aaaaggtttt atcaatactt atgtattgat aaaatataaa ttagggagag
      841 attcactttg aaaaagagaa cattatcggt tgttttgttt atacttgtga gtttaataat
      901 ttcaggctgc agcgtggatt taaacagtct tttgggtgat aatacggata acgattacag
      961 ctttttaaac aacgatgccg atgatatatt ggacaccggt ccggtaaaaa gcggtattct
     1021 aaacctgttt gccaccgctc ccgacacact taatccaata cttacggcca atgcttttgt
     1081 taaggattat tcttgttttg tatatgaaag tcttgttaaa ctgaatgaaa agcaaaagcc
     1141 tgtaccagtt ttggcagaaa gatgggagtc gtcagaaggc ggaaaggtgt ggactttcta
     1201 tttaaaggac aatatttatt ggcatgataa tatacgcttt tcggcggagg atgtggaatt
     1261 tacagcgggt accatagtga atgccggtgc aaacagccct tatgccgcca gctttaaaaa
     1321 tgttgtaagc ttttcggctg ttgactccaa aaccttcaag gttgaaatgg acagtcccaa
     1381 ttcctttact ccagagctta tgacttttcc cataattccc aaacactatt accttggaga
     1441 agacattatg aacacggaga aaaataacag gcccattgga acgggaccgt ataaatttgc
     1501 ggagtataaa gagaatgagt ttgttaagct tcaaagcaac gaaaactggt ggaataacac
     1561 tgatacggat gaggacgggc ttacccttcc atatattcag gaaattaata taaaaatatt
     1621 cggaaaggac agctctgtaa taaacgcttt tcaatccaag gaagttgacg taataattgc
     1681 cgacacgaag aattggatag gttacagcgg aagatcggat ataaccctta aaaagtactt
     1741 gagcaacagg tttgagttta ttgccttcaa cctgtcaaac aagatactga aagaaaagga
     1801 agttagaacg gcgatagcct atactgttga ccggggtaag cttgtgaatg atatcatgcc
     1861 cggtgaggct gtggtgtcgg acttgcctgt aattccggat acctggctgc atgatacgaa
     1921 cgtcatatcc tatgaaaggg atattgaaaa agcgaaacaa atccttgaag acagtgggtg
     1981 gaaggagaaa aacggtgttt tatacaagag aataaacggc gttaacactc cccttacgct
     2041 ggaattattg gtaaatacgg ataatgaacg caggcttgag gtggcagagg aaatcaaaga
     2101 ccagctcaaa gaagcaggaa tagttctgaa tatcaaaagt gtaacatggg aggatgaact
     2161 taataaagtc aaatccggta agtttgacat ggcttttata gggtgcaccg tgccctcaat
     2221 accggacata tcctttttgt actcatcggc tgaaatcgga acaggaataa atattgccgg
     2281 ttacgaaaat gagagtgttg acaattattt gtctcagatt ttgcgggaga ctgactcatc
     2341 aaggaagaaa gcatattttt tcaacatgaa ggaggcgata aacgaggata tgccgtatct
     2401 gggattgtat ttcttttatg atgcggccat atataataaa agaatacgag gagagttcag
     2461 tccttgtata tgggataagt atgataattt cacaaaatgg tatatacctg atgtggaata
     2521 gattatgagt acatgtaatt aatgtgaaga taattcataa tatttcttgt gtgataaaaa
     2581 gagtataata ttattgataa aataaaagcg aaaggtaaat tgatatggtg tggataataa
     2641 taattgtttt gattttagca ttggatcaaa taaccaagtt tattgtgtca agtaattttg
     2701 aattaggcga tagtactaca attatagata atttttttca tattgttcat tggaggaaca
     2761 cgggtgctgc atggggaatc atgcaaaacg gcaagtatgt actggtaccg gttacgtttt
     2821 tgctggtgct gttaatggca tactttatgt ataaatacaa caacaagcta ttaagggttg
     2881 ccctcagcat ggttattgga ggagcattgg ggaaccttat tgacagggtg ttcagatccg
     2941 gtggtgttgt ggattttctt gatttccagt tcggcagtta tcattttccg tcatttaaca
     3001 ttgcggattc ttttgttgtg gtgggaacaa taataatggc ttattatttg ttttttatac
     3061 ataaggataa agaggaatca agttctaaaa agccagttga cgaaaaaaca acggagcaga
     3121 gggaaaatta gaacctttac agaaggtgat ttagcggtgc caaattttat aatagacatg
     3181 tatattgtta tggagaagta ataaagttat gaaggaaata aaattagtat gtgaagataa
     3241 aggaacaagg gttgatttat ggctttccga aaggcttgag gaatattcac ggtcttatat
     3301 aaaaaagatg atagacgatg aaatgcttct ggtaaacgga gagcatgtca aatccaatta
     3361 taagctaaaa tccggggatg aagtgctctt gaggataccc gacccgaaac ccttggatgt
     3421 ttgtgcggaa gatatagagg taccaatatt atatgaggat gaggatattg tggtggttga
     3481 caagccaagg ggtatggtgg ttcatccggc tgcaggcaat tattcgggaa ctctggtaaa
     3541 tgcacttatt ggttattgcg gtgataacct gtcggatata aacggcgtca taaggcccgg
     3601 tattgtccat agaattgacc gggacacctc cggtgtattg gttgtggcaa aaaccaacac
     3661 tgcccatgaa agattgtcgg agaagctcaa ggttcacgat atagaaaggg tttacgttgc
     3721 attagtctac ggcgttattg cggagaacca gggtaaaata gatgccccca tcggaagaca
     3781 ccctaccgac agaaaaaaga tggctgtcaa tgccaaaaac ggcagatctg ccgtaactca
     3841 ttttcgtgtg cttgaaagat tcaaggatgc aacttatatt gaggtcactt tagagaccgg
     3901 aaggacacat cagataaggg ttcacatgtc ctatatagga taccccatta ttggcgatac
     3961 tgtttacgga cgaaaaaacg accggtatga cattaacggg caggcacttc atgcgaaaga
     4021 gcttggtttt atacatccta cccgcgaaaa atatatggag tttgagtcgc ccttgccgga
     4081 atattttgaa aatctattgg aggaattgcg gcaaaaccga taagaaattt aaaatgattg
     4141 aatcattttt atttatctgt ttaattaagc aataatattc ctaatatatt gacattctaa
     4201 tttacataaa gtacaatcaa tataggtgct aaaagtaact aatgatgaag attaatataa
     4261 ataaagatat acataatttg tgttaataac agattgtgta cagagataat ggttgccttg
     4321 cacaataaat tctgggcgaa tcattataaa ggtgtttttt tgtatttggg gagtggtaaa
     4381 atgaaagaaa ttgagcaggc atatctttat gctgtttcaa aaagaaaaaa gtttgttata
     4441 atactgtttg cttgtgctat ttgtatagct atacttgctt tgcttgaaaa gattaaagaa
     4501 agcgatatgt tttcaatagt ggttatttct tgtataacca tttttttaat acttttgggg
     4561 atacttgtta ttaattctcc aaatataaag ggttccttta gatcttcgcg agtatataaa
     4621 tccttagaaa atttgggata tgcagatgaa tttattaaca ctattgatta tgaaattcaa
     4681 aaccagttga gaattaatta tcatgatgac gtttatcaca ttggtctttt t
//
LOCUS       DAOOJC010000155         4724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14252_length_4724_cov_3.820090, whole genome shotgun sequence.
ACCESSION   DAOOJC010000155 DAOOJC010000000
VERSION     DAOOJC010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14252_length_4724_cov_3.820090"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1883
                     /locus_tag="PLA01_05425"
     CDS             <1..1883
                     /locus_tag="PLA01_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPD00773.1"
                     /translation="PVPRITAKKRNINVGILILPAPAWDSINNKAVNDSNPDFSIMEL
                     LNRNINKPMDWELVNPHPPAFDKSEYKDIMHLNSSQISGIDNNSYSDFCAYFKDLKSV
                     AKAQTDFEADLTYQGTVGYTENINKSKFFKYWEVSLDDPNLTPEKIAQDYDILFQTSH
                     GEITYSKDVQDKVKAFLNSGGQLWWENCRGLVIESGDGFTEEVEFVSLNPGHNYKYPQ
                     IPVLDSEGKMHPLFDNIYTIDSEKATRVWAPGIYNKNSEISMLGDGEDWLNDDNRYID
                     NLQPDDNVILKIKNTDTGEILPNMAVRNVINDDAPAGRIVITTSDIGCGISKFADRGG
                     GKAVEDYKFCYNLFGWMSKISVSFDETTGNEWDGSNEFSVEATFTNHGAKTQVYDVTH
                     LYDSKSWNLVPTNDFENYKYDYPWIKSIDGMGYPNKIELKPNQSEVVTYKFNIKRTDL
                     HYYEFTVKAAESGVKYTRDTMETLYRLNNVRVEKPVFSGRQVNGTEARFDVTINAPKE
                     PDNDLRTENYELNLKIKKDGGFIDPQTVVNYVELKTDGNTPPLENYKYQYSVDKKGVL
                     YLKVIVEDTLITKPSEKIKFSIGLNNLSGGSYDVTGKIEVIDPVSRKRLAFSDEAIYK
                     IK"
     gene            2172..3071
                     /locus_tag="PLA01_05430"
     CDS             2172..3071
                     /locus_tag="PLA01_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPD00774.1"
                     /translation="MYKGKNALRRKRRLMFARIRTLVILVLAVWGVISFFQNSGEPKN
                     EDKPVGSHIDAVEDLNSINVGAYGQDINTDMEGYGYVPKTNSQNSESGFENSATGNEG
                     TDNQTSETVYIDDWRLILVNADNPLPDDYTFELSSLDETRKFDSRAIDELKMLVERCR
                     RETGGSIWAQSTYRDRNSQKTVFEQKVREYINSGKSREEAEELTATSISRPGTSEHET
                     GLAVDFNYAKTSFENTSVFKWLMEHAHEYGFILRYPDGKQSITKVKYEPWHFRYVGKE
                     HAQVIKSKEFCLEEYIDYLKSRE"
     gene            3140..3787
                     /locus_tag="PLA01_05435"
     CDS             3140..3787
                     /locus_tag="PLA01_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPD00775.1"
                     /translation="MVLWGVFISAFLIGFSGAMMPGPMLGITIDGSLKKGWTAGPLVV
                     LGHGILELSFITVMIFGLKDFFSNPIVAGIIGLFGGAFLAWMGYGMVRSGINKSVSLE
                     NQGTGNSSGIRNLVLAGLIVSATNPYFILWWASTGMESIRQAYTLGLMGIVFFFMGHL
                     LSDFSWYIMISVAFSRGKKLVSDAVYRWIIIFLGISIIAFSVYFMCSGWKMLRGL"
     gene            complement(3934..>4724)
                     /locus_tag="PLA01_05440"
     CDS             complement(3934..>4724)
                     /locus_tag="PLA01_05440"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPD00776.1"
                     /translation="TKPVYKNKKNLIGRIFTFKDITSYQKLFDEISVKNEELSAMNEE
                     LISLNEQLKEYSQSVEQLTLEKERNRFSRELHDTMGHTLTLLIAMLKATRINYSKVPS
                     KLSENLNDAIEIAQNGMNELRMAIAGLDSQTINKNNLYNSLKELTDRFSNLGVCIDLS
                     IHDDLNETLYDRKQLTSIIYRICREALTNSIRHGQAKSISIIINLASERIKLHIFDDG
                     KGCCKIAKNKGLTGMEERINSVGGAITFGSGEDGGFNINAVLPI"
BASE COUNT         1470 a          813 c         1035 g         1406 t
ORIGIN      
        1 taccggtacc gaggattacg gcaaagaaaa gaaacattaa tgtcggaatt ctaattttgc
       61 cggcacccgc gtgggattca ataaacaata aggcagttaa tgattcaaat cctgatttct
      121 ctataatgga gcttctaaac aggaatatta acaagcctat ggattgggag ctggtaaatc
      181 ctcatcctcc tgcatttgac aagagtgaat acaaggatat aatgcatctc aattcatccc
      241 aaatttctgg aattgataac aacagctaca gcgatttttg tgcatatttt aaagacctta
      301 agagtgtagc aaaggctcaa actgattttg aagcagattt aacctatcag ggaacagtgg
      361 gttataccga gaatataaat aagtctaagt tcttcaaata ctgggaggtt tcgctggatg
      421 atccgaacct caccccggaa aaaatagccc aggactatga catcttgttc cagacatccc
      481 atggtgaaat aacatactcg aaggatgtac aggacaaggt taaggcattc ttaaattcag
      541 gaggccagct gtggtgggaa aactgccgag gtcttgtaat agagtccgga gacggcttta
      601 ctgaagaggt tgagtttgtt tccctaaatc ccggccacaa ctataagtat cctcagattc
      661 ctgttttgga cagcgaaggt aaaatgcatc cgttgtttga caatatatat acgattgact
      721 ctgaaaaggc cacccgtgta tgggctccgg gcatatataa caaaaacagt gagatatcaa
      781 tgctgggaga cggcgaggat tggctcaacg atgataacag gtatattgac aacttgcagc
      841 cggatgataa tgtaattctc aagattaaga ataccgatac gggggaaata cttcccaaca
      901 tggcggtaag aaatgtaatc aatgatgatg cacctgcggg aagaattgtt ataactacaa
      961 gcgatattgg ctgcggtata tcaaagtttg cggatcgcgg cggcggaaag gctgtagaag
     1021 actataaatt ctgttacaac ctgtttgggt ggatgtcaaa gataagtgta agctttgacg
     1081 aaactaccgg aaatgaatgg gacggaagca atgagttttc tgtagaagct acctttacaa
     1141 accatggtgc taaaacccag gtatatgatg taacgcatct atatgattct aagtcatgga
     1201 atctggtacc taccaatgat tttgaaaact acaagtatga ttatccatgg ataaagagca
     1261 ttgatggaat gggttatccg aacaagattg agcttaaacc taaccagtcc gaagttgtta
     1321 cttataaatt caacattaag agaacagatc ttcactacta tgagtttacc gtgaaggcag
     1381 cggagtccgg tgtgaaatat acccgtgaca ctatggagac tctgtacagg ctcaataatg
     1441 taagagtaga aaaaccggta ttttcaggtc ggcaggttaa cggcacagaa gcacgtttcg
     1501 atgttacgat aaatgcaccg aaagaacccg ataatgacct gagaaccgaa aactatgagc
     1561 ttaaccttaa gatcaaaaag gatggaggct ttattgaccc gcaaaccgtt gtaaactatg
     1621 ttgagctgaa gaccgacggt aacacgcctc cgttagaaaa ctataaatat cagtactcgg
     1681 ttgacaaaaa gggagttttg tatctgaagg ttattgttga ggacacactt attacaaagc
     1741 cgagtgaaaa aataaagttc agtattggct taaacaacct tagcggcgga agctacgatg
     1801 ttacagggaa aatagaggta attgacccgg tttcccgtaa aagacttgca ttctccgatg
     1861 aagcaatata caagataaaa taagaatgct gtaaaagatg tttatggccc cgtgtataca
     1921 ccttagtgta cgcggggctt tataaactta ttttgatttt tcaggaggca catcgaatct
     1981 catgaatatt gcttttatac tacttttctt taatggttca attattataa aataacactt
     2041 ttaaaattac taaataagtg ataaaatctt taaatgggta gaaataaagc gttcggagca
     2101 tacgtattta atctaacggt tatgatggat taacacatat acatgttgag aggttgtaaa
     2161 gggagagaga aatgtacaaa ggaaaaaatg ctttaagaag aaaaagaagg ctgatgtttg
     2221 ctcgaatcag gacattggtt atacttgttt tggcagtatg gggtgtcatt agttttttcc
     2281 aaaattccgg agagccaaaa aatgaagata agcctgtcgg ttcgcatatt gatgcggtgg
     2341 aggatttaaa tagcattaat gtaggggcgt atggtcagga tattaatacg gacatggaag
     2401 ggtacggcta tgtccccaaa accaatagcc aaaattctga atccggtttt gaaaattccg
     2461 caacagggaa tgaaggcaca gataaccaaa cttcggaaac ggtttacatt gatgactggc
     2521 gtttaatatt ggtgaatgcc gataatccgt tgcccgatga ttacactttt gagctatcca
     2581 gcttagatga gacaagaaaa tttgattcaa gggccataga tgaattaaaa atgcttgttg
     2641 agcgttgcag acgtgagacc ggaggcagca tatgggcaca gtccacatat agggacagga
     2701 attctcaaaa aacagtattt gaacaaaaag ttcgggaata tattaattcg ggcaaatcga
     2761 gagaagaggc agaggaactc acagccactt ccatttccag acccgggaca agcgagcacg
     2821 aaaccggtct ggctgttgat ttcaattatg ccaagactag ttttgagaat acaagtgttt
     2881 ttaaatggct tatggaacat gcccacgaat atgggtttat tttaaggtat cccgatggca
     2941 aacagtcaat aacaaaggtt aaatatgaac cgtggcattt cagatacgtg ggaaaagagc
     3001 atgcacaagt tatcaaatca aaggaatttt gtttggagga gtatatcgat taccttaaaa
     3061 gccgggaata gtcaaagtca gaaacagttt gataagcata aacggaaatt ggccgtaaaa
     3121 attataaagg acgattaaca tggttttgtg gggagttttt attagtgctt ttttaatagg
     3181 cttttcgggt gcgatgatgc ctggacctat gttggggatt acaattgacg gaagtctcaa
     3241 aaaaggttgg acagcaggtc ctttggtagt attgggtcat ggaatcctgg aactgtcatt
     3301 tattaccgtc atgatctttg gactcaaaga ttttttttcc aacccgatag ttgcagggat
     3361 tattggattg ttcggaggcg cttttcttgc ctggatgggc tatggaatgg taaggtcggg
     3421 aataaataaa tccgtttctt tggagaatca agggacagga aacagctcgg gaataagaaa
     3481 ccttgtattg gcagggttaa ttgtcagtgc caccaatcct tactttattc tttggtgggc
     3541 gtcaacgggg atggaatcaa tacgtcaggc ttatacatta gggttgatgg gcattgtttt
     3601 tttctttatg gggcatctct tgtcggattt ctcatggtat atcatgatat ctgtggcatt
     3661 ttccagagga aagaaactgg ttagtgatgc tgtatatcgc tggattatca tttttttagg
     3721 tatatctatt atagccttct cggtttattt tatgtgcagc ggatggaaga tgctccgggg
     3781 actttaatgt ccatttcttt tattgactat catatatcat acaattaaat tgaaagtgct
     3841 gttatggaat acggcaatgt ttaaaagata tatgacacta tagatttata ttcggaaaga
     3901 aaacaatggc ttggtatata atttttgcat ccgctatatc ggcaggacag catttatatt
     3961 aaaccctcca tcctcgccgg agccaaaagt tattgccccg ccgacggaat tgattctttc
     4021 ctccattccg gttaagcctt tgtttttggc aattttacag catcctttgc cgtcatcgaa
     4081 tatatgcaat tttattcttt cgcttgctaa atttatgatt atggaaatgc ttttggcttg
     4141 tccatgccgt attgagttcg ttaaggcttc ccggcatatt ctatatatta tacttgtcag
     4201 ttgttttcga tcatataatg tttcattcaa atcatcatga atggataaat cgatacaaac
     4261 tcccaggtta ctgaatctat ccgtcaattc tttcaatgaa ttatacagat tgtttttatt
     4321 tatagtctgg gaatccaatc cggcaattgc cattcttaac tcgttcattc cattctgggc
     4381 aatttcaatt gcatcattaa gattttcact caacttggag ggaactttgg aataattaat
     4441 gcgggttgct ttcaacatag caatcagaag ggtcaatgta tgtcccatag tatcatgaag
     4501 ctcccttgaa aatctgtttc tttccttttc aagagttaac tgctctacac tttgtgaata
     4561 ctcttttagc tgctcgttca atgaaataag ctcctcattc atggcggaca attcttcatt
     4621 tttgacactt atctcatcaa aaagtttctg ataacttgtg atatctttaa atgtgaaaat
     4681 cctgccgatt agattttttt tatttttata taccggtttt gtgt
//
LOCUS       DAOOJC010000156         4712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14297_length_4712_cov_4.237492, whole genome shotgun sequence.
ACCESSION   DAOOJC010000156 DAOOJC010000000
VERSION     DAOOJC010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14297_length_4712_cov_4.237492"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2619)
                     /locus_tag="PLA01_05445"
     CDS             complement(<1..2619)
                     /locus_tag="PLA01_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPD00777.1"
                     /translation="MRKLQRFVALVLSFALCMQLVTFSVYYFEGGQTVFAAESFIYGD
                     LNGDNSLDSTDLTLLKRYLLRKISDFPVEDDLKSADLDGDGEINSTDLTLMKRKILRK
                     LEYFPVEKPTQTPTPTQMPTLPPTPTHPPTLTPTPIPTPTIEVPTVSDYLRPFVNIVT
                     SAQTVQTGSEVTITVYSEDDTEVVKTNLEINGNKHDIDSKGTAVFKPTTAGIYEVKAI
                     SEDAYGNVGVATAQIVCIDSSDKTFPQASLTEPADWSAVTGKTTIIGTASDENIVKYV
                     LEYSEKSKNEYVKIAEGNFNVKEGELGELDTSRMKNGLYDLRLTVFDKGGNTRSHAIT
                     VLVQSKLKKGVTSLSFEDMTIPAGRLPLTVIRSYSSNDKSTGDFGKGWTINTGIRLYK
                     SCTDGDNWKINKGVVYSIEETKPHLIIVTYPDGTMDKFQMMLDPNPFQPTSVYQGRMI
                     YKPVGETTSSLEPISSNICYAADIGFGFGVYSDLFEGTKYNPTRYRLTTKEGDVLVFN
                     ASGMLESYSDSNKNTVKVTSGGLTHSNKKSLKFTKDSKGRITKITCNEGNSYEYTYDK
                     YGDLVSVKDPLGNVTKYIYDENHDLIDVIDPRGIRITRNEYDSYGRLKAVYDAGGNKA
                     TYEFNDAAGIEIITDRLGNKTILEYDENENLVKLTDALGNSTTFTYDKDGRMLTRSDP
                     LNSYANIYYNHKGEIVGEIKDTGSSGSNGSVDEEIDESRRGKKDSLGNTVEFTFNSQG
                     KILTAKDIAGNITTNIYDAAGNLTQTIDPLSYSTSIGYDGKGNVTSITDQLGKKVQYN
                     YDDKGNLISQIDDTGIIMNFGYDENGRCVSKTVTLNTPEGVKTVTSRDIYDSMGRVVQ
                     SIDGKGGISKVEYNSIG"
     gene            complement(2949..4631)
                     /locus_tag="PLA01_05450"
     CDS             complement(2949..4631)
                     /locus_tag="PLA01_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6382 domain-containing protein"
                     /protein_id="HPD00778.1"
                     /translation="MDHVYLEKFEFGYESDASGSFLIVNADADEKVLTYQIEMIKNNP
                     NKNILPVSLIQKDSKYQFRYSITSRLTLSQYLKRNRLKRDEFINIFYDITKTILDAKV
                     YLLSDKAFIINKDYIYINPDSMEVALVYLPVNFDININDNLKNFTINFIMYGANIEES
                     SNDNFIQRILYLLKSDTFNILEFNNLLRELKAKRGIAQMDIHQYSDEGLSGVGNQSPD
                     VLEKEPFIQGREIVKPSVERPESKKPVITKPETAKSKAQKSLIPKPQIPKPQIPRPQT
                     PDSRIREARSNALIPNTNKDDNKSHPKGLKIKMRYKTSVIVIGGILQGIILICSIALL
                     TSGVMDSLENDMAINIIGVGILAGAASYLVWKTLLVEKNKVESISEIKESIIKESIAV
                     DKRQANEVNAKPIIMSDMKDKPSVEDKKPVIGREQSRAINLFEASDRTVIIQPKDYNK
                     TVILNSKERAFPYLEGKNSSAKEKIIINKPSFIIGRLPGKADYISKNNAVGKVHAEII
                     SRDGRYFVKDLNSLNGTFINAKRLTGNMEYEIKNNDTITFANSEYVFIVPGV"
BASE COUNT         1250 a          986 c          803 g         1673 t
ORIGIN      
        1 tcctatggag ttatactcca cttttgatat tccgccttta ccgtcaatgc tctgtaccac
       61 ccggcccatg ctgtcatata tatctcggct ggttacggtc ttgacccctt caggtgtatt
      121 caatgttacg gttttgctta cacatcttcc gttttcatca tatccgaagt tcatgattat
      181 tcctgtatcg tcaatctgac tgatcaggtt tcctttatcg tcatagttat actggacctt
      241 tttccccaac tgatcggtaa tacttgtaac atttcctttt ccgtcatacc ctatactggt
      301 agagtatgaa agcgggtcaa tggtctgtgt aagattaccg gctgcatcgt aaatatttgt
      361 agtaatattt ccggcaatat cttttgcggt aagaattttc ccctgactat tgaaggtaaa
      421 ttctactgta tttcccaagg aatccttctt acctcttctg ctttcatcaa tctcttcatc
      481 aacagagccg tttgaaccgg aagaaccggt atcctttatt tctcctacta tttctccctt
      541 atgattataa taaatgtttg cataagaatt cagagggtcg cttctggtca gcattctacc
      601 gtctttgtca taggtgaatg tagtgctgtt acccaatgca tccgttaatt taacaagatt
      661 ttcattttca tcatactcca atattgtctt gtttcccaag cgatcggtta ttatttcaat
      721 tcccgctgcg tcgttaaact catatgtggc tttatttcct cctgcatcat agactgcctt
      781 aagtcttccg tatgagtcgt actcattcct tgtaatcctt atacctcttg gatcaataac
      841 atcaataaga tcatggtttt catcgtatat atattttgtt acatttccta aaggatcttt
      901 taccgataca agatccccgt atttgtcata tgtgtattca taactgttac cttcattaca
      961 ggtaattttt gttatacgac ctttgctatc ctttgtaaac ttaaggcttt tcttatttga
     1021 atgagtcaat ccaccggatg ttacctttac agtatttttg ttgctgtcac tatagctttc
     1081 aagcattccc gacgcattaa acaccaatac gtctccctct tttgtggtaa gacggtaacg
     1141 ggtcggatta tatttcgtac cttcaaacaa gtcggaataa acaccgaagc caaagcctat
     1201 gtctgcagca tagcatatat tgctggatat aggttccaac gaagatgtgg tttctcccac
     1261 aggcttatag atcatcctgc cctgataaac cgatgtgggt tggaacgggt tgggatcaag
     1321 catcatttgg aacttatcca tcgttccgtc aggatatgtt actattatca aatgaggttt
     1381 tgtttcctca atagaatata ctaccccttt gtttatcttc cagttatcgc cgtcggtaca
     1441 actcttatat aaacgtattc cggtgttaat tgtccatcct tttccaaagt cacctgttga
     1501 cttgtcatta ctgctgtatg aacggattac ggtcaagggc aatctgcctg caggtattgt
     1561 catatcttca aaactcagac ttgttacgcc ctttttgagc ttgctttgaa ccaataccgt
     1621 tatcgcatgg gatcttgtat taccgccttt atcaaacaca gtaagcctta aatcataaag
     1681 accgtttttc attcgtgaag tatccagttc tccaagctct ccctctttta cattgaagtt
     1741 tccttcagca atcttgacat attcattttt tgacttttcg gagtattcaa gcacatattt
     1801 tacaatattt tcatcacttg ctgttccgat tatcgttgtt ttaccggtaa cggcagacca
     1861 atcggccggc tctgtcaatg atgcttgcgg aaatgtctta tccgaagaat ctatgcaaac
     1921 tatctgtgca gttgccacac ccacgttacc gtaagcatcc tctgatattg cttttacttc
     1981 atatatacct gccgtggtgg gtttaaatac tgccgttcct ttgctgtcaa tatcatgttt
     2041 attcccgttg atttcaaggt ttgttttcac aacctctgtg tcatcctctg aataaacggt
     2101 aattgttacc tcacttcctg tttgcaccgt ctgagcactt gtaacaatgt ttacaaacgg
     2161 ccttaagtaa tcggaaaccg taggaacctc aattgtaggc gtaggtatag gagttggggt
     2221 caaggttggg gggtgtgtag gtgttggggg aagtgttggc atttgtgttg gtgttggtgt
     2281 ttgagtcggc ttttctacag gaaaatactc caattttctc agtatctttc ttttcatcaa
     2341 tgtaaggtct gtactgttta tttctccgtc cccatctaaa tcagcggact ttaaatcatc
     2401 ttccacagga aaatctgaaa tctttctcaa tagatatctt ttaagcaaag ttaaatccgt
     2461 gctgtccaat gaattgtcac catttaaatc accatatata aagctctctg cagcaaaaac
     2521 cgtttggccc ccttcaaaat agtaaaccga aaaggttaca agctgcatac ataaagcaaa
     2581 agacaaaacc aaggccacaa atctttgtag tttcctcata acactaccat ccccttttgt
     2641 aaaaatagat aaaaaacaga atatgtatag ctggtatttt aacaattttt acaactttgt
     2701 gcaatcccaa aaagcaaaat tttagaatga taatatattt tttaaatttg ccggcagcaa
     2761 tcaggtgtaa attatttaaa tgaaaaaacg tgaaagaacg gtataaaaaa agtatagagc
     2821 ctgctaatgc aacgtggaga aagggagagc atattctcac gaagtgagtt ctgcccgacc
     2881 ccacgaagta tttacaggct ctgtactttc gaaattaaat ttccggaaca cttatcttca
     2941 aatttgtttt atacacccgg aactataaac acgtattcac tgttggcaaa ggtgattgtg
     3001 tcgttgtttt ttatctcata ctccatatta ccggtaagtc ttttagcatt tatgaaagta
     3061 ccgttgagtg aattcaaatc tttaacaaaa tagcgtccat ccctggatat tatctcggca
     3121 tgcactttac cgacagcatt gttcttgctt atgtaatccg ccttacccgg cagccttccg
     3181 ataataaagc tgggcttgtt aataattatc ttttccttgg cactgctgtt ttttccttcg
     3241 aggtaaggaa aagctctttc cttcgaattc aaaatcacgg ttttattata atctttcggc
     3301 tgaattataa ctgttctgtc agaagcttca aataaattaa ttgcacggga ttgttccctc
     3361 ccaatcacag gctttttatc ctcaaccgaa ggtttgtctt tcatatcgga catgattatg
     3421 ggttttgcat tgacctcatt tgcctgtcgt ttgtcaactg caatggattc ttttattatg
     3481 gattctttta tttcagaaat gctttcaact ttattctttt caaccagtag tgttttccat
     3541 accagataac ttgcagcccc ggccagtata ccgacaccga taatatttat tgccatatca
     3601 ttttccaatg aatccataac tccggatgta agcagtgcga ttgagcaaat caaaataatt
     3661 ccctgaagta tgccgccaat aactataaca ctggttttat acctcatttt gattttcagt
     3721 ccttttgggt ggcttttatt atcatcttta tttgtattgg gaatcaaagc atttgacctt
     3781 gcttcccgta ttcgcgaatc tggcgtttga ggtcttggaa tttgcggctt gggtatttgc
     3841 ggcttaggta ttagtgattt ctgggctttt gattttgctg tttctggttt tgttattacc
     3901 ggctttttgc tttcaggtct ttccacactg ggttttacta tctcacgccc ttgtataaac
     3961 ggttctttct ccaaaacatc cggggattga tttcccacac cgctcagtcc ttcatcagaa
     4021 tactgatgta tgtccatctg tgctattcca cgttttgcct tcaattccct caataaattg
     4081 ttaaactcca gaatattaaa ggtatccgat ttcaataaat ataagatcct ctggataaag
     4141 ttatcattac tgctttcttc aatattggct ccgtacatta taaagtttat tgtaaagttc
     4201 ttaaggttgt cgtttatatt tatatcaaag tttacaggaa ggtaaaccag agcaacctcc
     4261 atactgtccg gatttatata aatatagtcc ttatttatta taaatgcctt atccgataac
     4321 aggtaaacct tagcatcaag gattgttttg gtaatatcgt aaaaaatatt tataaactca
     4381 tcccttttca gtctatttcg tttaagatac tgagataagg tcaatctgga tgtaatgcta
     4441 taacggaact ggtattttga gtctttttgt atcaggctaa caggcagaat attcttattg
     4501 ggattgtttt taatcatttc aatctgatat gtaagtactt tttcatccgc atcagcattt
     4561 actataagaa aacttcctga tgcatcactt tcataaccga actcaaactt ttcgaggtaa
     4621 acatgatcca tagatttttc ctccttgaat taccaagaaa taatggcgct tcgctatttt
     4681 gtgtgggtaa acacactaat tagttaatta at
//
LOCUS       DAOOJC010000157         4708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14314_length_4708_cov_3.250806, whole genome shotgun sequence.
ACCESSION   DAOOJC010000157 DAOOJC010000000
VERSION     DAOOJC010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14314_length_4708_cov_3.250806"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLA01_05455"
     CDS             <1..406
                     /locus_tag="PLA01_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPD00779.1"
                     /translation="EGVDQRVLDKIVDEEISIGDYVLTGGELPAMVLVDAVSRLIPGV
                     LSSEESYSQESHYDGLLEYPQYTRPYEFKGRKVPDVLISGHHANITRWRRQQSLKRTY
                     LKRPDLLERLNLEKEDRELLKEALEQNCENIT"
     gene            571..912
                     /gene="rplS"
                     /locus_tag="PLA01_05460"
     CDS             571..912
                     /gene="rplS"
                     /locus_tag="PLA01_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPD00780.1"
                     /translation="MDIIKAIEQEQLKTDLPDLQIGDHVKVHLKVKEGNRERLQVFEG
                     NIIGMQGSGLKETFTVRRISYGVGVERVLPLHSPKIDHIDIVRRGKVRRAKLYYLRGR
                     VGKAAKVEVKL"
     gene            1096..1779
                     /gene="lepB"
                     /locus_tag="PLA01_05465"
     CDS             1096..1779
                     /gene="lepB"
                     /locus_tag="PLA01_05465"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPD00781.1"
                     /translation="MEYKKESFNEKDRHEKLEKTIETDVKAVKPNYLKEACKWLLLII
                     AAMFVALILRTYVFEWVVVDGKSMENTLHDEEVLFVSKINYRFNEPKRGDIIIIQISE
                     GNWDYLYFAKNFRALTTILPSQGEVNYIKRIVGLPGEKIELVDGYVYIDGVKQNEPYI
                     KGMTYGQDFDIPKYVPEDCVFVMGDNRECSKDSRQIGFIKLNQIKGKAVFRIKPFGKF
                     GSIYDYDNN"
     gene            1794..2687
                     /gene="ylqF"
                     /locus_tag="PLA01_05470"
     CDS             1794..2687
                     /gene="ylqF"
                     /locus_tag="PLA01_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HPD00782.1"
                     /translation="MNIQWFPGHMAKTRRILTDNHKLVDVVIELLDARIPLSSKNPEI
                     DRILNNKPRVVALNKADLADEKVSKQWSSWYNSQGYANIFIDSLKGTGIKELKRKMQE
                     LMKEKFERDRQRGRIFRPIRTMIVGIPNVGKSSLINRLAGKSIAVTGDKPGVTRGKQW
                     INVNNEIQLLDTPGILWPKFEDKRVGLNLAITGAIKDDILDIQELASVLLEILSVDYP
                     QYIKERYKLDTLEGKKGYELLEEAGRKRGCLVSGGQVDLTRISSIILDELRGGKIGRI
                     TLEKPGEDINRDGEEDDIKTD"
     gene            2656..3429
                     /locus_tag="PLA01_05475"
     CDS             2656..3429
                     /locus_tag="PLA01_05475"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPD00783.1"
                     /translation="MEKKMTLKQIELSVKELDIDEALEKLYLYKEEYGDKINSLIERY
                     GKKKLRQEQELNRYRAMCSYEDEAYQRGYEIIAGIDEAGRGPLAGPVVAAAGILRKGA
                     FIEGLNDSKKLTPQKRDELYDVIMKNALGIGVGVVDEKVIDEINILNATKEAMKKAVK
                     ELVLTPDILLIDSVELKDVKIPQVSIIKGDAKSVSIAAASIIAKVTRDRFIEKMDSKY
                     PEYGFAKHKGYGTKEHIEAIKKYGICPIHRMSFTKNFVG"
     gene            3471..>4708
                     /locus_tag="PLA01_05480"
     CDS             3471..>4708
                     /locus_tag="PLA01_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00784.1"
                     /translation="MRIEGSFDLLNSAGVSDIMSRLDVGDVVRAKVMEIAGNELMLKL
                     FDGTAVKATMMSSVDIKQGEIVEFVIKNKSENRLFLETLKNNGQKILSLEDDIKSKLL
                     ELGLKPDKRNIEAATELKNNNAPLDRETITKVLDAVVKFKDLSCDKAAFLVSKGISPG
                     EKAVQSLNQLAFGTKIEASLTELMDKLIGIDDKELLSRIGKSLESVDLKYEPVNPDSM
                     DLSPDTANQKSESLSTNLEKLPPEKQIESQIEAFGNKLEGFGVDKNLISQIKQKIFEF
                     TGNNNKGSIEPERLIEYLESNVPDFEALYDSNPKEIGKAIHNLMKELYPKEYIKGYQK
                     VYPKDESADKGASFENKPDVKLDTDADRIRRSFEKMFVRVSEDTKDLDVKKVYKSMYE
                     KLDTIAKALEASKVPGKGDIL"
BASE COUNT         1612 a          720 c         1174 g         1202 t
ORIGIN      
        1 tgagggcgtg gatcagcgtg tccttgacaa gatcgttgac gaggagatat ccatagggga
       61 ttatgtgctt accgggggcg agcttccggc aatggtgctt gttgatgcag tcagcaggct
      121 tattcccggt gttttatcca gtgaggagtc ttattcccag gaatcccact atgacgggct
      181 gctggagtat ccccaatata caaggcccta cgagtttaaa ggcaggaagg ttcccgatgt
      241 gctcatatcc ggtcatcatg ccaatataac ccggtggaga aggcagcaat cccttaagag
      301 aacctatctg aaaagaccgg atttgttaga aaggctgaac ctcgaaaaag aggacaggga
      361 gctattaaaa gaagcattgg agcagaattg cgaaaatatt acctaaaacc taagtttcaa
      421 aaagtttgga gcctgtctat acatataaaa agataataaa atggaaaaaa ggcatttaca
      481 actaggtgag tctatggtat aattttagct tgtggaatac gggcggtccg ctgttatata
      541 caagaacgtc tatgatatag gaggagaaaa atggatatta ttaaagcaat tgagcaggaa
      601 cagttaaaaa ccgatttgcc tgatttacaa attggagacc acgttaaggt acatctaaag
      661 gtaaaagagg gcaacaggga aagacttcag gtttttgaag gaaacataat cggaatgcag
      721 ggttccggac tcaaggaaac atttactgta agaaggattt cctatggtgt aggagtagag
      781 agggttcttc cccttcattc tccaaaaatc gatcatatcg atatcgtaag aagaggtaaa
      841 gtaagaaggg caaagcttta ttatctgcgt ggcagagtcg gtaaagctgc aaaagttgaa
      901 gttaaactgt aaagtaaaaa caaaggggga atttagcccc cttgtttttt gcacgttttt
      961 tcgaagtcaa actgaaaact gttatttctt ttcttcataa aaaaatagtg gtataattag
     1021 ttatataaaa gagaatcatt tataaccgaa agaggttata agtggagtga gaaatatatt
     1081 taagggttgt aggttatgga gtataaaaaa gagtctttta atgagaagga caggcatgaa
     1141 aagctggaga agacgattga aaccgatgtg aaagcggtaa agccgaatta tctgaaggaa
     1201 gcttgtaaat ggttgctgtt aataattgca gctatgtttg tagctctgat attaagaact
     1261 tatgtatttg aatgggtggt tgttgacggt aagtcgatgg aaaacaccct ccatgacgaa
     1321 gaggttttgt ttgtaagtaa aattaattac aggtttaatg agccaaaaag aggagatatt
     1381 atcattatac agatttctga agggaattgg gattatttat atttcgcaaa gaatttccgt
     1441 gcattgacta ctattcttcc aagtcaaggc gaagtaaatt atataaagag gatagtgggg
     1501 ctgccgggtg agaaaattga gcttgtggat ggatatgtgt atatagatgg ggtaaaacaa
     1561 aatgaaccgt atattaaagg tatgacctac ggtcaagatt ttgatattcc aaagtatgta
     1621 cccgaggatt gcgtttttgt aatgggcgat aacagagaat gcagcaagga cagcagacag
     1681 ataggtttta ttaagttaaa ccagataaag ggtaaagcag tctttagaat caagcccttt
     1741 ggcaaatttg gaagcatcta cgattacgac aataattaaa actggaggaa aaaatgaata
     1801 tacaatggtt tccgggtcat atggccaaga ccagaagaat tttaacggat aatcataaat
     1861 tggttgatgt ggtgatagag cttttggatg ctagaatacc acttagcagc aagaatccag
     1921 agatcgacag aattttaaat aacaaaccca gagtagtggc tttaaacaag gccgatttgg
     1981 ctgatgagaa agtttcaaag cagtggagta gttggtataa ctcacagggt tatgccaata
     2041 tttttataga ctccttaaaa ggcacaggaa taaaggaact caaaagaaaa atgcaggagc
     2101 ttatgaagga aaagtttgaa agagaccggc agaggggaag gattttcagg ccgatcagga
     2161 ccatgattgt cggcattccc aacgtcggca agtcgtctct tattaacagg cttgccggta
     2221 agagcattgc cgtcaccggc gacaaacctg gcgttacccg gggtaagcaa tggataaatg
     2281 taaacaatga gatacagctg ctcgatacgc cggggattct gtggcccaaa ttcgaagata
     2341 agagggttgg tttgaacctt gcaataacag gagccataaa ggacgatatt ctggatattc
     2401 aggagctggc ttcggttctt ttggagattc tttctgtgga ttatcctcaa tacattaaag
     2461 aaagatacaa attggataca ttggaaggga aaaaaggcta cgagctttta gaggaagcgg
     2521 gaaggaagag aggctgtctg gtatcgggag gacaagtcga tcttaccaga atttcctcca
     2581 ttattctcga tgagcttcga ggcggcaaaa tcggcaggat aacactggaa aaaccggggg
     2641 aggacattaa tagagatgga gaagaagatg acattaaaac agattgagtt gtcggttaag
     2701 gaacttgaca ttgatgaagc attagaaaag ctttatttat ataaagagga gtatggcgat
     2761 aaaatcaact cgcttattga acggtatggg aagaaaaagc tcaggcagga acaagagtta
     2821 aaccggtacc gtgccatgtg ttcctatgaa gatgaagcat atcaaagggg ttatgaaata
     2881 atcgcgggta ttgacgaagc gggaagaggc cccctggcag gtcctgtcgt tgctgccgcg
     2941 ggtattcttc gaaagggtgc ttttatagaa ggactcaacg actcaaaaaa gctgactccc
     3001 caaaagaggg acgagctcta tgacgttatc atgaaaaacg cattgggtat cggagttgga
     3061 gttgtggatg agaaggtaat cgatgaaata aacatcctca atgctacaaa agaagcaatg
     3121 aagaaggcgg tgaaggaact tgttttaacc ccggacattc ttctgattga ttcggtagaa
     3181 ctcaaagatg taaaaatacc tcaggtttca ataataaaag gtgatgcaaa aagcgtatcg
     3241 attgccgcgg catcaattat tgcaaaggta acgagggacc gtttcataga aaaaatggat
     3301 tcaaagtacc cggagtatgg ttttgcaaaa cacaagggat acggaacgaa agagcatata
     3361 gaagctataa agaaatacgg tatttgtccg atacatagaa tgagctttac aaaaaatttc
     3421 gttggctaga atcaattttc agtttatggg aaaaattggg ggcatgaaaa ttgaggattg
     3481 aagggtcttt tgacttactt aatagtgcag gtgtttcgga tataatgtca cggcttgatg
     3541 taggagacgt tgtcagggca aaggtaatgg agattgccgg aaacgagctt atgttaaagc
     3601 tgtttgacgg cacggctgtt aaggccacaa tgatgtcttc tgtagatatt aagcagggtg
     3661 agatcgtgga gtttgtcata aagaacaaat cggaaaaccg gttgtttctg gagacattaa
     3721 aaaataacgg tcaaaagatt ctgagccttg aggatgatat aaaaagcaag ctattggagc
     3781 ttggtttaaa gccggataaa agaaatattg aagctgcaac ggaacttaaa aacaacaacg
     3841 ctccgttgga cagggaaaca ataacgaagg ttttggatgc agtggttaaa tttaaggatt
     3901 tatcctgcga caaggcagcg tttttggtat cgaagggaat cagtcccggg gaaaaagccg
     3961 tacaaagttt gaatcaactc gcttttggca caaaaataga agccagtttg acggaactta
     4021 tggataagct tatcggaatt gatgataaag aattgctttc ccgtattggc aaaagccttg
     4081 aatctgttga tttaaagtat gagcctgtta atcccgactc tatggattta agtcctgaca
     4141 ccgctaatca aaagagtgaa tctttgagta caaatcttga gaaactaccg ccggaaaaac
     4201 aaatagagtc tcaaatagaa gccttcggaa acaaactgga aggttttggc gtcgataaaa
     4261 atctgatttc ccaaataaaa cagaagattt ttgagtttac aggcaataac aacaaaggaa
     4321 gcattgagcc ggagaggctt attgagtatt tagaatccaa tgtaccggac tttgaggcac
     4381 tctatgattc caacccaaag gaaataggaa aagccatcca taatttaatg aaagaattat
     4441 atccgaagga atatataaag ggatatcaaa aagtatatcc aaaggatgaa agtgcagata
     4501 agggtgcttc ttttgaaaat aaacccgatg taaagcttga cactgatgcc gataggatac
     4561 gcaggtcttt tgaaaaaatg tttgtacggg tcagtgaaga tacgaaggat ttggatgtaa
     4621 aaaaggtcta taaaagcatg tatgaaaagc tggacactat agcaaaggcc cttgaggcat
     4681 cgaaggttcc ggggaaggga gacattct
//
LOCUS       DAOOJC010000158         4704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14331_length_4704_cov_4.055711, whole genome shotgun sequence.
ACCESSION   DAOOJC010000158 DAOOJC010000000
VERSION     DAOOJC010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14331_length_4704_cov_4.055711"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2761)
                     /locus_tag="PLA01_05485"
     CDS             complement(<1..2761)
                     /locus_tag="PLA01_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3846 domain-containing protein"
                     /protein_id="HPD00785.1"
                     /translation="MEGTDESKINEKLLKVLVVEQGKAPYVTEIRNGYKELQQQVGGA
                     FTTIEIAEGIDAVCADDVDFDSTPINRIVNGQPIFGTFLVARVNYNTGEYVSLTDADI
                     DKYFKQFEAPLIEITDLLQEFEDVKIRQEEKASEPNFQEIGFYRMMPVGQVSLPTAKT
                     AIQDNPIYSQIEDISKAHKVETIEAEPMQSKQMNLFDLTPKGSEEQLIEIVLLEGSVM
                     QDGKKRIYDFAMTNPTGSAFSAFLKAEYGVGGHSVNKQGIGFENHNSTGIEFDWADEN
                     GEKHKTNITWLRAAIVIRKLIDEGRYLDLQEVNIDQDEKFNSLKNELEEVKAELIEIE
                     KKWESGAIFSYKQSEYWQDLEDLKDELEKELAEIEQYHTPDEKDSEEKESKPQEQTAK
                     DIPLSVEAKSGQSRHTKLNFRYSEDYNLYPNGAKTKYKNNIEAIKLLKRIENEKRLAN
                     PEEQIILARYVGWGGLANAFSDTASGWENEHQELKVLLDEKEYEDARNSTITAYYTEP
                     ELVKCIYNAFERFGFKGGRDRKILDPAMGTGNFFSVLPEAIADTPLYGVEIDSITGRI
                     AKQLYQKANIYVQGYETTNFEDNSFDVVLGNIPFNNIKLYDKRYADEDFLVHDYFIAK
                     SLDLVKPGGIIGFITSKGTMDKRDTAVREYIAQRADLIGAVRLPNNAFKALAGTEVTA
                     DILFFKKLDRPRKVDKYSLPDWVFTNTRKTDYININQYFHEHPDMVLGEMRHSRNMYG
                     NEEGTACIAPEGQDLYAELDKAINKLHATFTAEADKPLEAAEEESVNEVTELDAPKGT
                     KNYTYVVLDEKIYYCEHNKLIPQDYTGKRAERIKGLCEIRGALLNVIAVQTRDYTPDE
                     LQKAQKKLNSVYDRFVDRCGFINDKSNIAVFSDDDQFPLLRSIEDQSEDKQSWVKAPI
                     FYKATI"
     gene            complement(2887..3249)
                     /locus_tag="PLA01_05490"
     CDS             complement(2887..3249)
                     /locus_tag="PLA01_05490"
                     /inference="COORDINATES: protein motif:HMM:NF025561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HPD00786.1"
                     /translation="MSKIDVSYTEIDGVNIPNIQISNDSKDDRPLGKYGRMAMEYLKE
                     QNPQRYMILKMDGTLMATMHRVQEEAVEKIEHITQQMLKSDPMPNTEDIMEKTRHMNS
                     LRSAAEEIVLNELIYQVR"
     gene            complement(3994..>4704)
                     /locus_tag="PLA01_05495"
     CDS             complement(3994..>4704)
                     /locus_tag="PLA01_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HPD00787.1"
                     /translation="PCIVKDSLSDEEALMYVVETNVIQRSFSDMLPSEKAAVLALRHS
                     KMFSQGKRNDIINELKRLENPEYKGIDETSAPVGQKLTSREKIANEYGLSKNTVARLL
                     RVDKLIVGLKSRVDCDEISLRCAVDLSYLADSEQEEVERVLSENEFKVDMKKTEMLRS
                     YSANSKLNEKTTYQILSGQINRKPKSSSAPSIKIKHKVYSKFFSPDTKVSEIEKVVEE
                     ALELYFQTHPDRKEANTA"
BASE COUNT         1189 a         1072 c          817 g         1626 t
ORIGIN      
        1 taatggttgc tttataaaat attggtgctt taacccatga ctgtttatcc tcgctctgat
       61 cctcaataga acgtaagagc ggaaattggt cgtcatccga aaatactgca atattggatt
      121 tgtcattgat aaacccacat cggtcaacga atctatcata tacgctgtta agttttttct
      181 gcgctttttg aagttcatca ggcgtgtaat cccttgtctg tacagcaata acatttaaaa
      241 gcgcacctct gatttcacaa agccctttaa tcctttcagc tcgtttccct gtataatcct
      301 gtggtatcaa cttgttatgc tcacaataat atattttttc atccaacacc acataggtat
      361 agtttttagt tcctttagga gcatccagct cagttacttc attcactgat tcttcctcag
      421 cggcttctaa cggtttatca gcttctgcgg taaaggttgc gtggagctta ttaatggcct
      481 tatcaagttc tgcatataaa tcttgccctt ccggtgcaat acaggctgta ccctcctcat
      541 ttccatacat attcctgctg tgtctcattt cacccaaaac catgtcaggg tgctcatgga
      601 aatattgatt gatgttgatg tagtctgttt ttctcgtatt ggtaaaaacc cagtccggca
      661 aagaatactt atcaaccttt cttggacgat caagcttttt aaaaaataaa atatcggccg
      721 taacttccgt ccccgcaagt gctttaaatg cattgttcgg caaccggaca gcaccaatca
      781 ggtcggctct ttgtgctata tattccctta ccgcagtatc tcttttgtcc attgttcctt
      841 tacttgtgat gaaaccaatg ataccgcccg gttttacaag atccagggat ttggcaataa
      901 aataatcgtg aaccagaaaa tcctcatcag catatctctt gtcgtacagc tttatattat
      961 tgaatggaat attcccaagg accacatcaa aagaattatc ctcaaagttt gtggtttcat
     1021 acccctgcac ataaatattg gctttttgat atagttgctt tgcaattcgt ccggtgatac
     1081 tgtcaatctc aacaccgtaa agaggcgtgt cggcaattgc ttcgggaagg actgagaaga
     1141 agtttcccgt ccccatcgct gggtcaagaa tctttctatc cctgccacct ttaaaaccga
     1201 acctctcaaa ggcattatag atgcatttta ccagttccgg ctcggtataa taagccgtga
     1261 tagtggaatt tctggcatcc tcatattctt tttcatcaag taagactttt agttcctgat
     1321 gctcattttc ccatcctgat gcagtatcag agaaggcatt agcaaggcca ccccatccca
     1381 cataacgtgc aaggataatt tgttcttcgg gattagcaag gcgcttttca ttttcaatcc
     1441 gctttaagag tttgattgct tcaatgttat ttttatattt ggtttttgct ccgtttggat
     1501 ataggttata atcctcggaa taacggaaat tcaacttggt atgacgggac tgtccagact
     1561 ttgcttcgac agataaggga atatccttag ccgtttgctc ctgcggtttg ctttcttttt
     1621 cttccgaatc cttttcatcc ggtgtatggt attgttctat ttctgccagt tccttttcaa
     1681 gttcatcttt caagtcttca aggtcctgcc aatactccga ctgtttgtaa gaaaaaattg
     1741 ctcccgattc ccacttcttc tcaatctcaa tcaactcagc ctttacttct tcaagctcgt
     1801 ttttaagcga gttaaacttt tcatcttggt ctatatttac ttcctgtaaa tccaaatacc
     1861 gtccttcatc aatcagtttt cggataacta tagcagctct cagccatgtt atattggttt
     1921 tatgcttttc tccgttttca tcagcccagt caaattcaat gccagtgctg ttgtggtttt
     1981 caaatccaat cccttgttta tttacagaat gacctccaac cccatattca gctttcaaaa
     2041 acgcactaaa tgctgagcct gtcggattag tcattgcaaa atcataaatc cgctttttac
     2101 catcttgcat tacactacct tcaagaagca caatttcaat aagctgctcc tcgctgcctt
     2161 ttggtgtcag gtcaaacagg ttcatttgtt tactctgcat tggctctgct tcaatggttt
     2221 caactttatg agcttttgaa atatcttcaa tttgagaata gatggggtta tcctgaattg
     2281 ctgttttagc tgtaggtaga gatacttgac ctaccggcat cattctataa aagcctattt
     2341 cctgaaagtt tggctcgctt gccttttcct cttgccttat ttttacatcc tcgaattctt
     2401 gcagtagatc agtaatttca atcaatggag cttcaaattg cttaaaatac ttatcgatat
     2461 ccgcatcggt caaagaaaca tattcacctg tgttataatt cactcttgca actaaaaatg
     2521 ttccaaaaat cggttgtccg tttactatgc ggttaatagg agtgctgtca aagtccacat
     2581 catcagcaca taccgcatca ataccttctg caatttcaat agtagtaaaa gcacctccga
     2641 cttgttgttg aagctcctta tatccattac gtatttcagt aacatagggc gctttaccct
     2701 gttctaccac caaaacttta agtaattttt catttatctt tgactcatca gttccctcca
     2761 tttcttgaag ttcatcaggg atattataag ctgcaattgt aacattttca ggcataaaaa
     2821 aagagccatc cgttaatgac tcattctttt cggatagctc tacatttgga atataaggtt
     2881 cactgcttat ctcacttggt aaatcagttc gttcagaacg atttcctccg ctgctgatct
     2941 caggctgttc atgtgtctgg ttttctccat gatgtcctcg gtgttcggca tcggatcgga
     3001 cttcaacatc tgctgtgtga tgtgctctat tttctccact gcttcctctt gtacccggtg
     3061 catggtcgcc atcagagtgc catccatttt caaaatcata tatcgctgtg gattttgttc
     3121 tttgagatac tccattgcca tcctgccgta tttccccagt ggcctgtcgt ccttgctgtc
     3181 gttcgagatt tggatattgg gaatattcac tccgtctatc tctgtgtaac tcacgtctat
     3241 tttgctcatt ttctgctctc ctttctttct cgatgatttt tatgttcctc tcaatttcga
     3301 tcaagattcc tttagaaata tcggtgacag tatagccgag ttttgcaatt aaaggaatgg
     3361 tattaaagtg agcaactgta gccatcccgt atcctaattc catttcttca tcagttaatc
     3421 cgcatctttt ccctacaaag taacaaacac tttcgctgat aagttcttga agctgcattt
     3481 ccagtccgtc ttgcggaagg tcttcaaaca gatgattccc aaagtcgttc tgaaagtttt
     3541 gcaggtaagt gtccaggttt tccataacag cttcataaga gattctgcga aggcagtctg
     3601 caaaacctga tgtctttatg ttatgcccat atgaaagacg cagctcaata cgcttcttca
     3661 tatcttcatc aagctgccag cttgttgcaa tcacatctct gccattggtc tgtgaaacat
     3721 caaacaagtg cttgatagta agtcttgcgt tttcatattc gcatacagca atacctgtcg
     3781 cacccttgtt gacatatctt ccaaccttat tccaaactac tgtaggtgca agcattgtaa
     3841 catttgggtt ttgagcatat acaagcagtg cattatcaaa actgtatttg aagaatttag
     3901 atgcaaactt aaggtaatct cgccaggcaa tctcgctccg ggtaatatgg ggagcgattg
     3961 tctcatagat cgtttttaac ctttcatctt ttctcaagca gtgttagcct cctttctgtc
     4021 agggtgggtt tggaaatata gttcaagtgc ttcttccacc accttttcaa tttcacttac
     4081 cttggtatca ggcgaaaaaa acttggagta aaccttatgt ttgattttaa tcgatggagc
     4141 tgatgatgat tttggttttc tgtttatttg acctgataga atctgatatg tagttttctc
     4201 attaagtttg ctatttgctg aatagctcct aagcatttca gtctttttca tatccacttt
     4261 gaactcgttt tcagaaagaa ccctttcaac ttcttcttgc tccgaatcag caaggtatga
     4321 aagatcaacc gcacatcgca gggaaatttc atcacaatca acccggctct tcaagccgac
     4381 aatcagttta tcaaccctca gtaagcgggc tactgtattc tttgaaagtc catattcatt
     4441 tgcgattttc tccctgcttg ttaacttctg ccccactggg gcagaagttt cgtcaatccc
     4501 tttatattca ggattttcga gcctttttaa ctcgttgatg atatcatttc ttttgccctg
     4561 agagaacatt ttagagtgcc tgagtgcaag tacagccgct ttttccgatg gaagcatatc
     4621 tgaaaatgat cgctgaatta catttgtttc taccacatac atgagggctt cttcatcaga
     4681 caagctgtcc tttacaatac aagg
//
LOCUS       DAOOJC010000159         4695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14364_length_4695_cov_3.360991, whole genome shotgun sequence.
ACCESSION   DAOOJC010000159 DAOOJC010000000
VERSION     DAOOJC010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14364_length_4695_cov_3.360991"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="PLA01_05500"
     CDS             complement(<1..110)
                     /locus_tag="PLA01_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose pyrophosphatase"
                     /protein_id="HPD00788.1"
                     /translation="MLGGNIVNKQGLTEEQFLTAYDASKFDRPSVTVDMLV"
     gene            complement(342..2951)
                     /gene="clpB"
                     /locus_tag="PLA01_05505"
     CDS             complement(342..2951)
                     /gene="clpB"
                     /locus_tag="PLA01_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPD00789.1"
                     /translation="MNIDKFTEKAQAAISATQDVAVRMGHQHVDGEHIHFALVTQEDG
                     LIPKLIGYMGLNVQMYAKDVETELEKLPKVSGSGASGIYATRRFNEILIHAEDEAKRF
                     KDEYTGVEHIFMALLKEKDTPSQKIFKRYGITLEKFMAGLGKVRSNQRITSKNPEETY
                     DALKRFGRDLVELARLGKLDPVIGRDAEIRRAIRILSRRTKNNPVLIGEPGVGKTAVV
                     EGLAQRILKGDVPEGLKDRTIFALDMGALIAGAKYRGEFEERLKAVLNDVNKSDGRII
                     LFIDELHNIVGAGKAEGTMDAGNILKPMLARGELHCIGATTLDEYRKYIEKDAALERR
                     FQPIVVDQPTVEDTISILRGLKERFEIHHGVRITDSAIIACSVLSNRYISDRFLPDKA
                     IDLMDEAAAMIRTEIDSMPTELDEIQRKIMQLEIERQALKKEEDEISRDRLLTLEKEI
                     SSLKEKSDRMKAQWEMEKENIKREKELKSQIEEVKRQIEEAERTYDLDKLAILKHGKL
                     PELQKSLEEAKQRKKTSENTLLKEEVTEEEIAEIVSRWTGIPVTKLVENEKDKLLNLE
                     GILHKRVIGQDEAVNSVSNAVLRARSGLKDPRKPIGSFIFLGPTGVGKTELAKALSEA
                     LFDSEDNVVRIDMSEYMEKHSVARLIGAPPGYVGYEEGGQLTEAVRRKPYCVILFDEI
                     EKAHPEVFNVLLQLLDDGRLTDSQGRTVDFKNTVVIMTSNIGSQHLLSGIKNNSEIDE
                     NTRNVVMGELNRHFKPEFLNRVDEIVMFKSLKIEEIIKIIDLVLKDIQKRLNDRRIRL
                     NASKTAKQHMAENAYDPVFGARPVKRYVQKHIETAIAKKIIAGEITEDADINIDVEGG
                     KLTVVAKKSKSGC"
     gene            complement(2974..3906)
                     /locus_tag="PLA01_05510"
     CDS             complement(2974..3906)
                     /locus_tag="PLA01_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPD00790.1"
                     /translation="MQYKDYYKILELDKNASSEKIKKKYRKLAKKYHPDANPGNKQAE
                     EKFKEINEAYEVLGDEEKRKKYDTFGSAYNFQNGYDFDPSQYGFGNNVRYEYRTSSSN
                     SYSDFFNMFFGGDGFDFDSIFNFTGGTNRRVRTPQKGEDIEAELEVTPEEGFRGVEKK
                     ISIRSREGVKSLSFKIPKGVKDGERIRLRGQGESGFHGGEKGDLLISVRLKSSERFQL
                     EGSDLVMVMNVMPWDAALGGEATVDTIDGKIVVKIPKGIQTDNRIRVAGKGYIEKTGR
                     RGDLYIRVRIMNPDYITREMKGLYEKLKTASQGR"
     gene            complement(4196..>4695)
                     /locus_tag="PLA01_05515"
     CDS             complement(4196..>4695)
                     /locus_tag="PLA01_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPD00791.1"
                     /translation="SKSKTIREIIFMEVSFMFGLVPFGRRGIGRRNGDLFDIDNFFED
                     FFNEPTFPVFLGNGGNMRVDISENEKEYVLEAELPGIRKEDVSIEVNDDRLTISANRD
                     ERVEEKRDNYIRKERRASRMTRSFSVDNVDTDKITAKHDNGVLTLILPKKEQSRPGGK
                     KIDIS"
BASE COUNT         1136 a         1232 c          731 g         1596 t
ORIGIN      
        1 accagcatat caaccgttac tgacggtcta tcaaatttgc ttgcatcata agccgttaaa
       61 aactgctcct ctgtcaggcc ctgcttgtta actatatttc cacccaacat tatcacccca
      121 tcactgatat cttctgtttg atattatcac tttattaata tcattataat gtttgtagac
      181 gcatttgtca agggttttta cctcagcaaa atcttcgaca aataccatga tatttttcat
      241 ttgatgtacg aaaatttatc tattaactta aaaatttcct tgaaccgtgg tgtaataaaa
      301 aacacatgaa aaatacactg ctgcattctt catgtattta attaacaccc agactttgat
      361 tttttagcaa caaccgtaag ttttccacct tccacatcta tatttatatc tgcgtcttct
      421 gttatttctc cggcaataat ttttttagct attgccgttt caatgtgctt ttgaacatac
      481 ctctttacag gtcttgcacc aaacacggga tcatacgcat tttcagccat gtgctgcttt
      541 gccgttttcg aagcattgag cctgattctt ctgtcgttga gccttttttg aatatccttt
      601 aaaacaagat caatgatctt aataatctcc tctattttca aagacttgaa catgacaatc
      661 tcatctacac ggtttaaaaa ctccggctta aaatgtctat taagttctcc cattacaaca
      721 tttcttgtat tttcatcaat ctcactgttg ttttttattc cgctcaaaag atgttggctg
      781 ccgatgttcg aggtcattat aacaacagtg tttttgaaat ccacggtcct tccctggctg
      841 tcggtcagcc ttccgtcatc caaaagctgc aataacacat tgaaaacctc cggatgtgcc
      901 ttttcaatct catcaaagag tattacacaa tagggttttc tcctcaccgc ttcggtaagc
      961 tgcccgcctt cttcataacc cacataacct ggaggtgcac caataagcct tgcaaccgaa
     1021 tgcttttcca tatactccga catatcgatt ctaaccacat tgtcctcgct gtcaaaaaga
     1081 gcctcggata atgcctttgc cagctcggtc ttaccgactc ccgtaggtcc aaggaatata
     1141 aacgaaccaa tgggctttct cgggtcctta agtccggatc ttgccctcaa aacagcattg
     1201 cttactgagt ttaccgcttc atcctgcccg ataacccgct tatgcaatat accctcaagg
     1261 tttaaaagct tatccttttc attttccaca agctttgtta ccggaatacc tgtccatcgt
     1321 gatacaattt cagcaatctc ttcttcagta acctcttctt ttaaaagagt attttcactt
     1381 gtctttttgc gttgctttgc ttcctccagg ctcttttgca gctccggaag ctttccgtgc
     1441 tttaaaatag ccagtttatc aaggtcatac gtcctctcgg cctcttcaat ttgtcttttg
     1501 acctcttcta tctgagactt cagttctttt tcccgcttta tgttctcctt ttccatctcc
     1561 cattgagcct tcattctatc ggatttttcc ttcaaggatg aaatttcctt ttccaaggtc
     1621 aaaagcctgt cccgggatat ttcgtcttcc tcctttttca gagcctgcct ttcaatttca
     1681 agctgcatta tctttctctg aatttcatcc aattctgtgg gcatactgtc aatttcagtc
     1741 cttatcatag cagctgcttc atccatcaaa tcaatagcct tatccggcag aaagcggtca
     1801 ctgatatacc tgttggacag cacactgcat gcaataatgg cactgtcggt tatcctaacc
     1861 ccatgatgta tttcaaaccg ctcctttaat cccctcaaaa tcgagatggt atcctccacc
     1921 gtaggctgat ccactacaat gggctgaaac cttctctcaa gagcggcatc cttttctata
     1981 tattttctgt actcatctaa ggtagtggct ccaatgcagt gaagttcgcc ccttgctagc
     2041 ataggcttca atatattgcc ggcatccatg gtaccctctg cttttcctgc ccccacaata
     2101 ttatggagct catctataaa caaaattatt cgtccgtcgg acttgttaac atcatttaaa
     2161 acggccttga gcctttcttc aaactcaccc cggtattttg ctccggcaat caatgccccc
     2221 atgtcaaggg caaaaatggt cctgtccttt agaccctccg gcacgtctcc cttaaggatt
     2281 ctttgagcca agccctccac cactgcggtt tttcccactc caggttctcc tatgagcacc
     2341 ggattgttct tggttcttcg ggataatatg cgtatggcac gccttatttc cgcatccctg
     2401 cctattacag gatcaagctt gccaagcctt gcaagctcca ccagatcccg tccgaacctt
     2461 tttaatgcat catacgtctc ttcagggttt ttagatgtaa tcctctggtt gcttcgtacc
     2521 tttccgaggc cggccataaa cttttccaaa gtaattccgt aacgtttgaa tatcttttgg
     2581 gaaggtgtgt ccttttcttt taataatgcc ataaaaatat gctcaacacc ggtgtactcg
     2641 tctttaaaac gctttgcctc atcttcggca tgaatcaaaa tctcattaaa acgcctcgta
     2701 gcataaatac cggatgctcc gctcccggac accttcggaa gtttttccaa ttctgtctca
     2761 acatcctttg catacatctg gacatttagt cccatataac cgatcaattt gggaataagt
     2821 ccgtcttcct gggtcaccag ggcaaaatgg atatgttccc cgtcaacatg ctggtgcccc
     2881 atcctcaccg cgacatcctg agttgctgaa attgctgcct gtgccttctc agtaaattta
     2941 tctatgttca ttgccatccc tcctctctgt gttttatctc ccttgtgaag ccgtctttag
     3001 cttttcataa agccctttca tctcacgggt tatataatcc ggattcataa tcctgaccct
     3061 gatatataaa tcgcctcttc tccctgtctt ttcaatatat ccttttcccg caacccgtat
     3121 cctgttgtcg gtttgaattc ctttaggtat tttaaccaca atctttccgt ctatggtatc
     3181 aactgttgcc tctcctccta aggctgcatc ccagggcatt acattcataa ccattaccag
     3241 gtcgcttccc tcaagctgga aacgctctga ggatttcagc ctgacggaaa tgagcagatc
     3301 gcccttttca cctccatgaa aacccgactc accctgtcct ctgagtctga ttctttcccc
     3361 gtcctttaca cccttgggaa ttttaaagga caggctctta acaccctctc tgctccttat
     3421 ggatatcttc ttttccactc ctcgaaagcc ttcctcggga gttacttcca gttcagcttc
     3481 aatgtcttct cctttttgag gtgttctcac tcgcctgttg gtcccaccgg taaaattaaa
     3541 tatactgtcg aaatcaaagc cgtctccgcc aaaaaacatg ttaaaaaagt cactgtagct
     3601 gtttgaactg ctggttctat attcatacct tacattattg ccgaacccat actgtgaagg
     3661 atcgaaatcg tatccgttct ggaagttgta cgcacttcca aaagtatcgt atttctttct
     3721 cttctcctca tctcccaaaa cctcgtaggc ttcattgatt tccttgaatt tttcttctgc
     3781 ctgcttgttt ccgggatttg catcaggatg gtatttttta gccagctttc tgtatttctt
     3841 cttaatcttc tcagaagaag cattcttatc aagctcaaga attttgtaat aatccttgta
     3901 ctgcataaag cacctccctt gcaaaagttt tatataccag atatctatgc tgtttcaaat
     3961 ccataatata ctattgtgct tgccttgaga aggggagaaa ctactgtgtt ttttctttca
     4021 aaaaaaatgc tgaacaaaat ctccgggggt atcaagcccc catcgctttt tggtcatttc
     4081 acttcgtgaa atcccactaa ataatgtctg cttcccttgc aaattctact tcttgcaagg
     4141 gaagcttgtg gattaatgat gacattacaa ctttacgtca tatcatattt ccatattatg
     4201 aaatatctat tttctttcct cccggtctgc tctgttcttt cttcggaagt atcagagtca
     4261 gaacaccgtt gtcatgcttt gcagtaatct tgtcggtatc aacattgtca acagaaaatg
     4321 accttgtcat cctgctggct ctccgctctt ttctgatgta attgtctctt ttttcttcaa
     4381 ctctttcgtc ccggtttgca ctaatggtca gcctgtcatc atttacttca atactgacgt
     4441 cctcttttct gattcccggc aactcagcct caagtacgta ttctttttca ttttcactga
     4501 tatccaccct catattgcca ccgtttccta agaatacagg aaatgtcggt tcattaaaaa
     4561 agtcctcaaa aaagttgtca atgtcaaaca gatctccatt tcttcttcct attcctcttc
     4621 ttccaaaagg tactaatccg aacataaacg acacctccat aaatataatt tccctgattg
     4681 tctttgattt tgacc
//
LOCUS       DAOOJC010000160         4686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14397_length_4686_cov_4.182898, whole genome shotgun sequence.
ACCESSION   DAOOJC010000160 DAOOJC010000000
VERSION     DAOOJC010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14397_length_4686_cov_4.182898"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..305)
                     /locus_tag="PLA01_05520"
     CDS             complement(132..305)
                     /locus_tag="PLA01_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00792.1"
                     /translation="MFIAALTETVIGAGKTVEFEEELDMESYKDVIGKAKYLKAYIVG
                     TSEDCEIEKTVTR"
     gene            complement(368..2602)
                     /gene="pcrA"
                     /locus_tag="PLA01_05525"
     CDS             complement(368..2602)
                     /gene="pcrA"
                     /locus_tag="PLA01_05525"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HPD00793.1"
                     /translation="MDLLEGLNKEQREAVLHGDGPLLILAGAGSGKTRVLTHRIAYLI
                     KEKNVHPASILAITFTNKAAREMRERIDRLVENNSDYIWVSTFHSMCVRMLRRDIDKI
                     DYDKSFVIFDYADQQTVIKDCLKELNLNDKNYPPKSMLEMVGRAKDELITPDIYSKMY
                     AQDFRMSKVAEVYQLYQKKLKQNNALDFDDIIMLTIKLLLDNPQVADYYQRKFRYILV
                     DEYQDTNTAQYSLISILAQGYKNLCVVGDDDQSIYGWRGANIRNILDFEKEFKDAKVI
                     KLEQNYRSTQTILDAANHVIKNNLGRKNKSLWTDKKGGDKIKYLECSNEHEEAFLAAS
                     EIKRLKTEKNRPYKDFAVLYRVNAMSRVIEEMFMREGISYKIFGGLKFYDRKEIKDVL
                     AYLRVIQNPADNISLKRIINEPKRGIGNVTIETAEQLANKRGVSIFTIISSAQDIPEL
                     SRSWAKLEKFVSLINSFRAQSLVMTASEMIEEVIERTGILSGYEQENTVEAQTRIENI
                     KELISVAMEFENENEEKSLMDFLAHVSLVSDVDTMDEDSDYVTLMTLHSAKGLEFPVV
                     FMVGMEEGIFPGYRSMTSETELEEERRLCYVGITRAMESLYMTSTFSRTLFGNTTFNR
                     VSRFLKEIPEEYFEANANESEAKTVNNSTAMGQKPGHKNGVAAGGFDPTVSFNTTSFK
                     TPQNKGGTVLLNVGDQVRHKMFGEGIITHKEKDGDDFVLEIHFKGKGMKRLMAGYAKL
                     EKIN"
     gene            complement(2730..3263)
                     /gene="raiA"
                     /locus_tag="PLA01_05530"
     CDS             complement(2730..3263)
                     /gene="raiA"
                     /locus_tag="PLA01_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPD00794.1"
                     /translation="MKFIVSGKNIVVTEALRERAIKKVGKLDKYFQRDTEAQITMSVQ
                     KNRHIVEVTILFDGGLLRAEEDNTDMYASIDKVVDSLERQIRRNKTKLEKKFHDGGLR
                     LENVKFDEKDVDEEHKFEIVRTKRFAIKPMTVEEAVLQMNLLGHEFFMFSNAETQEAN
                     VVYRRKDGNYGLIEPEF"
BASE COUNT         1294 a         1101 c          674 g         1617 t
ORIGIN      
        1 cccgtggcta gactttcact agctagatta gtgccatgcc tggcacacaa ttaaggctgt
       61 accaaaaatc attccggcac agccttaatc aaacattctt atgtaaaacc taaatatcct
      121 ctgttttatt tctacctcgt aaccgtcttt tctatctcac aatcctcact tgttcctaca
      181 atataagcct tcaaatactt tgcctttcca ataacatctt tatagctttc catgtcaagc
      241 tcttcttcga actccactgt ctttcctgca cctatcaccg tctcggtcag tgccgctata
      301 aacaaccaaa aggctgttat ctttctcttc tttcagtggt aaatcaggtt cgagtgaaat
      361 gaaaatatta atttatcttt tccagcttag cataaccagc catcagtctc ttcataccct
      421 ttcccttaaa gtgtatctca agaacaaagt catctccgtc cttttccttg tgggttatga
      481 tcccttcacc aaacatcttg tgccttacct gatctcccac gttaagcaat actgtaccgc
      541 ccttattttg aggagtctta aaactcgttg tattaaagct tacagtcgga tcaaagcctc
      601 cggcagccac cccgttttta tgccccggct tttgccccat tgctgtacta ttatttactg
      661 tctttgcctc gctttcattt gcattggctt caaaatactc ctccggaatt tctttcaaaa
      721 accttgaaac cctgttgaaa gttgtatttc caaacagtgt acggctgaaa gtgcttgtca
      781 tgtaaagaga ctccatggct ctcgtaatac ccacatagca tagccttctt tcctcctcaa
      841 gctccgtttc gctggtcatt gacctgtatc ccgggaaaat cccttcttcc atgcccacca
      901 taaaaacaac gggaaactca agtccctttg cactgtgaag ggtcataagt gtcacataat
      961 cactgtcctc atccatggta tccacatccg aaaccagaga tacatgagcc agaaaatcca
     1021 taagactctt ttcttcattc tcgttttcaa attccatggc aacggatatc aattctttaa
     1081 tgttttctat cctggtctgt gcctccacag tattttcctg ctcatagccg cttagaattc
     1141 cggttctctc aattacctcc tcaatcattt ccgaggcggt cattactaga ctctgagccc
     1201 gaaagctgtt tataagcgat acaaacttct ccagcttggc ccaggaccgg ctaagctcag
     1261 gaatatcctg ggccgatgaa attattgtaa atatgcttac ccctctttta ttggccaatt
     1321 gctctgcagt ctctatagta acatttccaa tccctctttt tggctcattg ataattcgct
     1381 tgaggcttat gttgtctgcc gggttctgaa taacacgaag ataggccaga acatccttta
     1441 tttcttttct gtcatagaat ttgagtcctc caaatatctt atagctaatc ccttccctca
     1501 taaacatttc ttctataaca cgggacatgg cattaaccct gtacagtacg gcaaaatcct
     1561 tatatggcct gtttttctct gtcttgagcc tctttatctc acttgcagct aaaaaagctt
     1621 cctcgtgctc gttggagcac tcaagatact taattttgtc gccgcctttc ttatccgtcc
     1681 acaggctctt gttttttcgt cccaggttgt tctttataac atggttggcg gcatcaagaa
     1741 ttgtctgggt tgaacggtag ttttgctcaa gctttattac ctttgcatcc ttaaactcct
     1801 tttcaaagtc taaaatattt ctaatgttgg cacccctcca tccgtaaatg gactggtcat
     1861 catcacccac aacgcaaagg tttttgtatc cctgtgccaa aatacttatg agcgaatatt
     1921 gggcggtatt ggtgtcctga tactcgtcca ccaggatata gcgaaatttt ctctgataat
     1981 aatctgccac ctggggattg tccaacaaaa gctttattgt aagcattata atatcatcaa
     2041 agtccagtgc attattctgt ttaagctttt tctgatagag ttgataaacc tcggcaacct
     2101 tactcatcct gaagtcctgt gcatacattt ttgaatatat gtcaggagtt atcagctcat
     2161 cctttgctct tcctaccatt tcaagcattg acttaggagg gtagttctta tcattgagat
     2221 taagttcctt aaggcagtcc tttataacgg tctgctgatc cgcataatca aatataacaa
     2281 agctcttgtc ataatcaatc ttgtctatat ccctcctcag catcctcaca cacatggagt
     2341 ggaaggtgct gacccaaata tagtcactat tattctcaac aagcctgtct atcctttccc
     2401 tcatttctct tgcagccttg ttggtaaagg ttattgccag tatacttgcc ggatgcacgt
     2461 ttttctcctt gatcaggtat gctattctat gggttagaac ccttgtcttt ccactgcctg
     2521 ctccagccaa aataagaagc ggtccgtcgc catgcaatac ggcttccctc tgttctttgt
     2581 ttaagccttc cagtaaatcc attaaatatc ttcctctcac ttggtgaaat tccatcaatt
     2641 aaaatgccga gactttagtc tcggcatttt aattatagta gaaaactcct ttgatatcaa
     2701 atatataatt aacgcttaga atccaataac taaaactcag gttcaattaa gccgtaattt
     2761 ccgtcttttc ttctgtaaac aacatttgct tcctgcgttt ccgcatttga aaacataaag
     2821 aactcatgtc cgagcaaatt catttgcaaa acagcttctt caacggtcat tggctttatg
     2881 gcaaacctct ttgtcctcac aatttcaaac ttatgctctt catcaacatc tttttcatcg
     2941 aacttaacat tttcaagtct aagacctcca tcgtggaatt tcttttccag cttggtcttg
     3001 tttcttctta tttgcctttc gagactgtct acaaccttgt caatagaagc atacatgtcc
     3061 gtgttatcct cttcggccct aagtagtcct ccatcaaaaa gaattgtaac ctccactata
     3121 tgccggttct tctgcacact catggttatt tgagcctctg tatccctttg aaaatatttg
     3181 tcaagtttgc cgactttctt aatggccctt tcccttaaag cttcagttac aacaatgttt
     3241 tttccgctaa ctatgaattt catatatgcc cacagcccct ttcagtcaac gacaaatatt
     3301 gcttaataaa ccctagtgca atacctgtct ttcttttcat taatattata ccccaaaacc
     3361 ctttgtgtaa aacactcctt tgtctgaaaa cctcaaaaaa tacccacctc caatttttca
     3421 ttaattaaaa tttaagagca aaagatttcc agacaactga caactaaaaa ttggggataa
     3481 tatataatat aataattcta catcaagtaa aatattcctt ctgtaatcgg tacctatttt
     3541 gttctattac ataaattcta aaaccccttt atataaaggt ttattacaat tcacaaacca
     3601 taaaacgcgt ctttcaatat aaaaaataac attaaaaaga caaaggcaaa acatgtagat
     3661 tgtttttctc ccggcaaggg ttaaaaagct ttacaatccg cacagaaatt atacccgtac
     3721 cattttccac aattatccca catttgcccc acaaagcacc tgttttgtgc acataaattc
     3781 tgtggataat gtggacaatt ctgtgaataa ctaatatagg cttgttttac ctgtggataa
     3841 ccctgttgtt atcttagtaa ttcgtatctt ttatgttaac tgactcttca cgagattttt
     3901 ctccgattta attaaacttt atgggaaatg cctataaaca ttaccttata ggattttatt
     3961 tccaaagtca tctttgtttt ttggtttggt atggcaaaaa aagaatagaa acgccctaat
     4021 tatgtaacac aaactaattg ttgtgttgct atcttattta tcaaaaattc gtcttcaatt
     4081 ctttattttt ttgttgaatt tgtatattgt tttagtccct cacatttgcg ataataagag
     4141 tgtaaacaga aaagaaaatt attgggtgtg atgcataaag atacaccaac tacaaaaaaa
     4201 taaatacacc tattaaaaag caatacaacg aatttattat ttaaaacaga cgaaacaata
     4261 tattttttat actacatctc attcattctt tattatatac tttgtactac tctttacatc
     4321 tgacacgaac tcaacataat cgactttact ttgcaaattc gggccaaaag atatcattac
     4381 accctttcct ttatatattg ttacgatttt gctaactacc gaaacttatg gtttcggttt
     4441 ttttattttc tttttgcggc atcgagccct gctactctac ttttcgacca tttcaattcg
     4501 tgaaatctta ttaaaaaagc gaaaggagta ttatctcctc tcgctcttta ttactacact
     4561 aacttaatat attctttagt cctctttcta ttactctgtc aaacgttttt caagagcttc
     4621 aagctgtgct ttcaattctt tcggaagctt ttccccgaat ttagcataat gctctttaat
     4681 ggattc
//
LOCUS       DAOOJC010000161         4663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14511_length_4663_cov_3.283420, whole genome shotgun sequence.
ACCESSION   DAOOJC010000161 DAOOJC010000000
VERSION     DAOOJC010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14511_length_4663_cov_3.283420"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1345)
                     /locus_tag="PLA01_05535"
     CDS             complement(50..1345)
                     /locus_tag="PLA01_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPD00795.1"
                     /translation="MKILIKNADIITCNASNDVIKNAFLGIKDGYIDFIDTTGEALKG
                     FNADKIIDADNKLVMPGLVNAHTHSGMTILRNFANDLALEEWLFNNIFPVEAKLTPED
                     IYWGTLLGIAEMIKSGTTTFADMYLYMEQVARAVYETGIRANLCRSPLKDGDGSVEDS
                     IRCFDYFREWNNGANGRMKVYIEVHSVYLYDEDTLRTSAKIAKELNTGIHIHVQETVK
                     ECEDSIKKYGMSPAQICCETGIFDVPVIAAHCVHLSERDMDIIKKKGVNVIHNPTSNL
                     KLGSGIAKINEMLDSGINVALGTDGAASNNNLNMMEEMHLASILHKGMHRDPLLINAP
                     GVLRMATLNGAKALGFEGKIGAIEKGMKADIILIDTDKPHLCPLNDPVAAVVYSAQGS
                     DVDTVIIDGVVVMEKGELKTIDEEKVKFMVRETAKRVLK"
     gene            complement(1424..2668)
                     /locus_tag="PLA01_05540"
     CDS             complement(1424..2668)
                     /locus_tag="PLA01_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPD00796.1"
                     /translation="MKSVIRDISLAKSGKQKINWVRKNMPLLRSLEEEFSKTKPFKGI
                     RVTVSVHLEAKTAYLAKVLAIGGGEVSVTGSNPLSTQDDVAAALADDGLNVYAWHGAT
                     DEEYKEHLNLALGYKPNIIIDDGGDLVNLLHTSRSELLPHVMGGCEETTTGVIRLKAM
                     EREGVLKFPMVAVNNAYCKYLFDNRYGTGQSVWDGINRTTNLIVAGKNVVVVGYGWCG
                     KGIAMRAKGFGADVIVCEVDPIKAAEAVMDGYKVMPMEEAAKLGDIFITVTGCIRVIH
                     RDHFKLMKDGAILCNAGHFDVEVCMTELEEMAVSKVEQRKNIMGYQMEDGRWINVLAE
                     GRLVNLAAGDGHPAEIMDMSFALQALSAEYVLKNYKDLGIRVLDVPEEIDRRVAVMKL
                     ESWGVKIDELTEEQKKHLDSWC"
     gene            complement(2672..3499)
                     /locus_tag="PLA01_05545"
     CDS             complement(2672..3499)
                     /locus_tag="PLA01_05545"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPD00797.1"
                     /translation="MDNIYAKVKETAGFIKERIKEIPQIAIILGSGLGPLADEIEGKM
                     EIDYKDIPNFPLTTVEGHAGKFVYGYLGNKKVLAMKGRFHHYEGYDVSQIVFPIRTFK
                     VLGVDNLIVTNASGGINKAFKPGDLMIIKDHISFLAPSPLRGRNINEFGLRFPDMSKA
                     YSPELVELCKKSAVKTGVDVKEGVYAFAQGPMYETPAEIRALGILGADAVGMSTVPEV
                     IAARHANINVLGISCITNMAAGILDQPLCHEEVMRTAKEAENNFTRLVKQVISDWEV"
     gene            complement(3727..>4663)
                     /locus_tag="PLA01_05550"
     CDS             complement(3727..>4663)
                     /locus_tag="PLA01_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPD00798.1"
                     /translation="SSLGILLLGASGNYYYIFSVMLVYSFFQTPMVPISETITLEHIS
                     SRGWKYGPIRLAGTLGYAIMPVLAGALIKNNTNNMFFLCFVLGIMTFLSVFMLPKIKG
                     HQSSGEKRSLWELFKNRELMLLLGFTIVTQITIGYYNSFFSIYYKEMGADNRILGWAT
                     FISCMSEIIFLVIGDKIIKRFGIKLTMICAAFVAVIRWLLMGMVNNVYFILALQFLHG
                     FTFIVIAYSMALYINEKVQDELKASGQALNSAVGTGFTRIIGSIAGGALSDYIGIRNV
                     FVLSSVAVLISIIVFVPVFVAISRKSGIKNSRGAY"
BASE COUNT         1331 a         1198 c          740 g         1394 t
ORIGIN      
        1 ttaaaatatt aattccaaaa agcatttttg tcaagagcaa tgcatttttt tacttaagaa
       61 ccctctttgc tgtttctctg accataaact taaccttttc ctcgtctata gtcttcagtt
      121 ctcccttttc cataacaaca acaccgtcaa ttataacggt atcgacatcg gagccctggg
      181 cagaatatac cacagctgca acaggatcat ttaacgggca gagatgaggc ttatccgtat
      241 ctataaggat tatgtccgcc ttcattcctt tttcaattgc cccaatttta ccttcaaacc
      301 ccaatgcttt cgcaccgttt aatgttgcca tcctcaacac gccgggagca tttatcagca
      361 aaggatccct gtgcatccct ttgtgtagta ttgatgcaag gtgcatttct tccatcatat
      421 tgaggttgtt attgcttgca gcaccgtccg tcccaagagc cacatttata cccgaatcca
      481 gcatctcatt aattttagct attccgctgc caagcttcag attgctggta ggattgtgaa
      541 tcacatttac acccttcttt ttaataatgt ccatatccct ttccgacaaa tgaacacagt
      601 gggctgcaat aaccggaaca tcgaaaattc cggtctcaca gcaaatttgg gccgggctca
      661 ttccatactt tttaatgctg tcctcgcact ccttcaccgt ctcctgaaca tgtatatgaa
      721 taccggtatt caattcttta gcaatctttg ccgaggttct caaagtatcc tcatcataga
      781 ggtaaaccga atgaacctca atgtaaacct tcatccttcc attggcaccg ttattccatt
      841 ccctgaaata atcaaaacac cggatagaat cttcaacact tccgtctcca tctttaaggg
      901 gacttctgca taaatttgcc cttatgcctg tctcgtaaac agcccttgcc acctgctcca
      961 tataaaggta catatcggca aatgtggtag tgcccgactt aatcatttcc gctattccca
     1021 gcaatgtacc ccaataaata tcctcgggag tgagtttggc ttcgacagga aatatattat
     1081 taaaaagcca ttcttccagg gcaaggtcat tggcaaaatt ccttaatata gtcattccgc
     1141 tatgggtgtg agcatttacc agtccgggca tcaccagttt attatcggca tcgatgattt
     1201 tatcagcatt aaagcccttt aaagcttccc ctgtggtatc aataaaatcg atatacccgt
     1261 cctttattcc taaaaaagca tttttaatca cgtcatttga agcattgcaa gtaattatat
     1321 ccgcattttt aataagtatt ttcactgtca aagtctcctc cgcttaataa aaaatccatg
     1381 ggtttttcta taacatcttt ggacatgtag ttacatattc gcatcagcac cagctatcca
     1441 ggtgtttttt ctgctcttcg gtgagctcgt caatcttaac cccccaggac tcaagcttca
     1501 tcacggcaac tcttctgtca atttcctcag gcacatccaa caccctgatt cccaaatctt
     1561 tatagttctt taacacatac tcggcactta aagcctgaag ggcaaagctc atatccataa
     1621 tttccgctgg atggccgtct cctgccgcaa ggttgacaag tcttccctca gcaagaacgt
     1681 ttatccacct gccatcctcc atttgatatc ccattatgtt tttacgctgc tctacttttg
     1741 aaaccgccat ttcttccaac tctgtcatgc atacctccac atcgaaatgt ccggcattgc
     1801 acaatatggc accgtccttc ataagcttga aatgatcgcg atgtattacc ctgatgcaac
     1861 cggttacggt aatgaagata tcccccagct ttgctgcctc ttccataggc attaccttat
     1921 atccgtccat aacagcctcg gccgccttta taggatcaac ttcacacaca ataacatcgg
     1981 caccgaatcc ctttgccctc atggcaatac ccttgccgca ccatccatat cctacaacaa
     2041 caacgttctt gcctgcgact atgagattgg tggttctgtt tattccgtcc cataccgact
     2101 gaccggtacc gtatctgtta tcgaacaaat acttgcagta agcattattt accgcaacca
     2161 tggggaactt taaaacgccc tctctctcca tagcctttaa ccttataacc ccggttgtcg
     2221 tctcttcaca tcctcccatt acatggggta aaagctccga ccttgaagtg tgcaacaggt
     2281 taaccaagtc tccgccgtca tcaataatga tattgggctt gtaccccaaa gcaagattca
     2341 gatgctcctt atattcctcg tcagtggcac catgccaggc ataaacatta agtccgtcat
     2401 ccgcaagggc agcagcaaca tcatcctgag tggacaacgg attgcttcct gttacagaaa
     2461 cctctccacc cccaatagca agaacctttg ccaaatatgc cgtcttagcc tcaagatgaa
     2521 ccgatacggt tactcttata cctttaaagg gcttagtttt tgaaaactct tcttcaaggc
     2581 ttcttaaaag gggcatattc tttcttaccc aattgatttt ctgctttccg gactttgcaa
     2641 gacttatatc acgaattaca cttttcatca cttatacctc ccaatcggat atcacctgtt
     2701 ttactagacg cgtaaaatta ttctccgcct ctttggccgt tctcataacc tcttcatggc
     2761 acaggggctg gtcaagtatt cccgcggcca tattggttat gcatgaaatt cccagcacat
     2821 taatatttgc atgccttgcg gcaataactt ccggcacggt ggacataccg acagcatcgg
     2881 cacccagaat acccagtgct cttatctccg caggagtctc atacatgggg ccttgagcaa
     2941 aagcatatac cccctcctta acatccaccc ctgtctttac ggcagacttt ttgcacaatt
     3001 caacaagctc cggactgtat gccttgctca tatccgggaa cctcaaccca aactcgttta
     3061 tatttctgcc tcttaacgga gaaggtgcta agaaactgat atgatctttt ataatcatca
     3121 aatcccccgg tttaaaagct ttatttattc cgccagatgc attggtaaca attagattgt
     3181 caactcccag cactttaaac gtccttatgg gaaacacaat ctgagaaaca tcgtatccct
     3241 catagtggtg aaagcgtccc ttcatggcaa gcaccttttt gtttcccaga taaccgtaca
     3301 caaacttacc ggcatgtcct tcaacagtgg tcaaaggaaa attcggtata tccttataat
     3361 caatctccat cttgccctca atttcatctg ccagtggacc aagtcccgat cccaggataa
     3421 ttgcaatttg gggaatttcc ttaattcttt ctttgataaa cccggccgtc tccttcactt
     3481 ttgcgtagat gttgtccata tcttacctcc cgctggtaaa tttatattat aatgcacatt
     3541 attattctag atattattcc atcaattatt gtaattaatt atcataacat ttattatatt
     3601 aacacatctt accttccgga aacaagacaa actacgtcct tagggtgcat taaataaacg
     3661 taaagaaaat gtctctataa aacttaagtt tcttttgcat ttttctttac ataaataatg
     3721 tgcaccctaa tatgcacccc ttgaattttt aataccggac tttcttgaaa tcgccacaaa
     3781 aacaggtaca aaaacaatta ttgaaataag aacggcaaca gagctcagta caaaaacatt
     3841 cctgattcca atataatcac tgagtgcacc tccggcaata cttccgataa ttcttgtgaa
     3901 tcccgttccg acagcggaat tcagtgcttg tccggaagcc ttcaattcgt cctgcacctt
     3961 ttcatttata tacaatgcca tagaatatgc aattacgata aatgtgaatc cgtgaagaaa
     4021 ttgaagtgcc aatataaaat aaacgttgtt aaccattccc ataagcagcc atctaattac
     4081 tgcaacaaag gctgcacaaa tcatggttag cttaatccca aaccttttta ttatcttgtc
     4141 tccgattact aaaaaaatta tttcactcat gcaggaaata aatgttgccc atccaaggat
     4201 acggttatct gcacccattt ccttataata tatcgaaaaa aacgaattgt aataacctat
     4261 tgtaatctgg gtaacaatag taaaacccaa aagcagcatc agttcccggt tcttaaaaag
     4321 ctcccaaagg gatctctttt cccccgacga ctgatgtccc tttatcttcg gcagcataaa
     4381 gactgacaaa aacgtcataa tccctaatac aaaacataaa aaaaacatgt tgttggtatt
     4441 gttctttatc aacgcaccgg ccagaaccgg cattatagca tatcccaaag ttccggcaag
     4501 acgtatggga ccatacttcc agcctcttga agatatatgc tctaaagtaa tggtttcact
     4561 tatcggaacc attggggttt gaaaaaaaga ataaacaagc attacggaaa aaatataata
     4621 ataattgcct gaagccccta gaagcaatat tcccaagctg ctc
//
LOCUS       DAOOJC010000162         4653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14544_length_4653_cov_3.479339, whole genome shotgun sequence.
ACCESSION   DAOOJC010000162 DAOOJC010000000
VERSION     DAOOJC010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14544_length_4653_cov_3.479339"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PLA01_05555"
     CDS             <1..499
                     /locus_tag="PLA01_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPD00799.1"
                     /translation="HTRNLKLQPLDYYFEKALKERLEIVSLVEEIEVKQKNLEVLTKA
                     RANEIYTEIRKEYADVSRELETLEVKLEQAKYDVENNIKSAYIDVKREGYNLRNMQET
                     LNMQKRSYEMLKNQYEHGFISKITVDEVALGIEELQSNLDIVIYSYNTKIMKLENASG
                     LGPAY"
     gene            515..1603
                     /locus_tag="PLA01_05560"
     CDS             515..1603
                     /locus_tag="PLA01_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPD00800.1"
                     /translation="MKKKLSILFLTLPVVLALTTALAVEANLINSINGFQKFAVENSR
                     QATIDDLQIKTLESALEDVKHDADFQPVPGTKIDMLNNGIKKEVAPLEAETNLEYAKR
                     SKEENAKKLKLDIYKAALDKILAQKQLETEASKLEIIKGKYSMFEARYKEGKITENDL
                     NDVKFALDTKEIDRDKAQKNLEIADLELKRLLNVELNTEEVVVEDKLDLEEWRPINIE
                     EAVKKAIENDIEIYKKSEDLKAREKTMEITEKYYKEGNFSYDDNKVNLETARIDLEDA
                     KINLEVEVRNKYNSLLTAKDNLELAAQWKRIQDKKLETAQVKYDQGLLSMEALLDQKE
                     KCLDAEYQRLVAIHDFNILKAELEALCE"
     gene            1642..2382
                     /locus_tag="PLA01_05565"
     CDS             1642..2382
                     /locus_tag="PLA01_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD00801.1"
                     /translation="MIIDIKNLDKVYKTGKIEVSALKKVNLTVEKGELLSVMGPSGSG
                     KSTLMNILGCLDRATSGSYILDGMEISKLNEVELARIRNLKIGFVFQSFNLLPKMTAL
                     KNVELPMVYARISAKERKKRAEYALERVGLKDRMHHKPNEMSGGQKQRVAIARALVND
                     PAIILADEPTGNLDSFSGEEIMAVFQGLNREGVTIVLVTHEPDIAQHTNRIVSFRDGR
                     LMTDIRVQQPIDANDVMKKLRVEMEAVT"
     gene            2379..3644
                     /locus_tag="PLA01_05570"
     CDS             2379..3644
                     /locus_tag="PLA01_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPD00802.1"
                     /translation="MKKVITVIVILAVIGGLIGLSYFISNNMSEVFSQGTVYSVKAAE
                     IGKDSISSSITASGIVEEVESFDIYVDNPTKVNKLLVKKYQKVSKGQQLMELDIESLE
                     NELDKLQINKKVQELSKNSPTIDAEIKRAESAVTSAEKALSDCEENYEKSKKLFEAQA
                     ISKNDLDMAEKAVTDAKTALDNAKVAYDAAVKNRNVDRDVKEENLNATILGIAELEKR
                     IKNAKESMICPVDGVIVEMNIQEGAYTSNVQPIFKIVNLDKLQVKAEINEYSIKDVKE
                     GQKVVITGDAIDENIEVLGVVESIFPVAKKNMSSGGEEVVVEVVISIDNINAALKPGL
                     SVDCKIMTLEKQDIVVVPIGALKIDKDENEYVLKIDKEKGVMVRQDVKLGIISDMMAE
                     VLDGLAEGDLIVDDPQSFHKDGSKVKILE"
     gene            3664..>4653
                     /locus_tag="PLA01_05575"
     CDS             3664..>4653
                     /locus_tag="PLA01_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD00803.1"
                     /translation="MSFLESIAQALESLRANKLRSILTMLGVIMGVFSIITIMAIGNA
                     TEEYIDSQFERIGANLLQIGYKNITVDRGEWLQIEDIATIKKAAPEIRNVSTIIQNMG
                     SLRMDTKTRDALVYGTTAQYKDFTPMEMAAGRFLSDFDVSSRTKVVVVDEYFAKRYFN
                     KVDVVGEIVKFKTPSGSNYNLKIIGVTKAENDLMAGMLDNENMPTYIYMPITTVQQMY
                     NNNKRLDSIMVSLDNDVDYTDVGNRIVRALELSKGKEDIYMSYSTQDTQKIFSSIIGV
                     ISGVLLVIAVITLIVGGIGIINILLVSVTERIREIGIRKALGAQKKDIVLQFIT"
BASE COUNT         1621 a          683 c         1153 g         1196 t
ORIGIN      
        1 acataccaga aatttaaaac tgcagcctct ggattactat tttgagaagg ccttgaaaga
       61 gagacttgag attgtcagtt tggttgagga gattgaggta aaacagaaga accttgaggt
      121 tctgacaaaa gccagggcaa atgagattta tacagaaata aggaaagagt acgccgatgt
      181 ttcaagagaa cttgaaaccc ttgaagtaaa gcttgaacag gccaagtatg atgtggaaaa
      241 caatattaag tctgcataca tagatgtaaa aagagaggga tataatttaa ggaatatgca
      301 ggagacattg aatatgcaga aaaggagcta tgaaatgctt aaaaaccagt atgaacacgg
      361 tttcatatct aaaattacag tcgatgaagt ggcactggga atagaagaat tacaaagtaa
      421 ccttgacatt gtgatttaca gttacaatac aaaaatcatg aaactggaga acgcgtcagg
      481 tctcggaccg gcgtattaga aagggtggtg tggcatgaag aaaaaattat caatattgtt
      541 tttgaccctt ccggtggttt tagctcttac aacagctctg gcagttgaag ccaatctaat
      601 aaatagtatt aacggttttc agaagtttgc ggtggaaaac agcagacagg ctacgattga
      661 cgatttgcag ataaagactt tagagagtgc tttggaggat gtaaagcatg atgcggattt
      721 tcaacctgtt cccggaacta aaatagacat gctcaataac ggaatcaaaa aagaggttgc
      781 acctttggag gcggagacga accttgagta tgcaaaaaga agcaaagaag agaatgctaa
      841 gaaactaaag cttgatattt ataaagcggc ccttgacaag atccttgctc aaaagcagct
      901 ggagacagaa gcctccaagc tggagattat aaagggaaaa tactcaatgt ttgaagcccg
      961 gtataaggaa ggaaagatta ctgaaaatga tctcaacgac gtaaaatttg ctctggatac
     1021 caaagaaatt gacagggata aagcacaaaa gaatctggaa attgcagacc ttgaactcaa
     1081 aagattattg aatgttgaac ttaacacaga agaggttgtt gtggaagata aattggattt
     1141 ggaagagtgg agacccataa atattgaaga agcggttaag aaggcaatag agaacgatat
     1201 cgaaatctat aaaaagagcg aagacctcaa agccagagaa aaaaccatgg agattacaga
     1261 aaagtactat aaagaaggca acttttcata tgatgataac aaggtaaacc ttgaaacagc
     1321 aagaatcgac ttggaggatg cgaaaataaa cttagaggtc gaggttcgga acaaatacaa
     1381 tagtcttctt actgccaagg ataatctgga actggccgca cagtggaaaa gaatccagga
     1441 taaaaagctt gagactgctc aggttaaata cgatcaagga ttattaagta tggaggcttt
     1501 acttgatcaa aaggagaaat gccttgatgc agaataccag aggttagttg ccatacatga
     1561 ttttaatatt ctgaaggctg agttggaagc attgtgtgaa taagcaccaa aaaagttctt
     1621 gtgaaaaggt atgtgatttg aatgataata gatatcaaga atttggataa ggtatacaag
     1681 accggaaaga ttgaggttag tgccctaaag aaggtaaacc tgaccgtaga aaagggcgag
     1741 ctgctatcgg ttatgggtcc ttccggctcg ggaaaatcaa ctcttatgaa catattgggt
     1801 tgccttgaca gggcaacttc aggcagttat atccttgacg ggatggaaat atcaaagctg
     1861 aatgaggttg aactggcaag gattcgaaac ttaaaaatag ggtttgtatt tcaatccttt
     1921 aaccttcttc cgaaaatgac ggcacttaaa aatgttgaac ttcctatggt atatgcccgg
     1981 ataagtgcca aggaaagaaa aaaaagggct gaatatgcct tggaaagggt aggccttaag
     2041 gacaggatgc atcacaagcc taatgagatg tccggcggtc agaagcagag ggtggctatt
     2101 gcaagggctc ttgtaaatga cccggctatt attttggcag atgaaccaac gggtaatttg
     2161 gacagctttt cgggcgaaga aattatggct gtgtttcaag ggcttaacag ggaaggggta
     2221 acaatagttt tagttactca tgagcctgat atagctcagc acaccaacag gattgtaagc
     2281 tttcgtgacg gaagattgat gactgatatc agggtgcagc agcctattga tgcaaatgat
     2341 gttatgaaaa aactcagggt tgaaatggag gcagtaacat gaaaaaagtg ataactgtaa
     2401 tagttatttt agctgtaatt ggcgggctta taggcttaag ctattttatc agcaacaata
     2461 tgagtgaggt gttcagtcag gggactgtat acagcgtaaa ggcggcggaa atcgggaagg
     2521 atagtatttc ttcctccata acagcatcgg gaattgtgga agaggttgaa tcctttgaca
     2581 tttatgttga caaccctacc aaggtaaaca agcttctggt taagaaatac cagaaggtat
     2641 caaaaggtca gcaattgatg gagctcgata ttgagtctct cgaaaatgaa ttggataagt
     2701 tgcaaataaa caagaaggtt caagaactgt ccaagaactc tccaactata gatgccgaga
     2761 taaaaagggc agagtctgct gtcacgagtg cagagaaggc actaagtgat tgtgaggaga
     2821 attatgagaa aagcaagaaa ctctttgagg cacaggctat atcgaaaaat gatctcgata
     2881 tggcagaaaa agctgtaacg gatgcaaaga cagcattgga taatgcaaaa gttgcttatg
     2941 atgctgctgt taagaaccgg aatgttgaca gggatgtaaa ggaagagaac ctcaatgcaa
     3001 ctatacttgg tattgctgag cttgaaaaaa gaatcaagaa tgctaaagag tccatgattt
     3061 gtcctgttga tggagtaatt gttgagatga acattcagga aggggcttat accagcaatg
     3121 ttcagccgat atttaagatc gttaatctgg ataagcttca ggttaaggcc gaaataaatg
     3181 aatacagcat taaggacgta aaagaaggtc aaaaggttgt tattactggt gatgctattg
     3241 atgagaatat tgaggtttta ggcgtcgtgg aaagcatatt ccctgtagcc aagaaaaaca
     3301 tgtcctccgg cggtgaagag gtagtggtgg aggttgttat ctcaatagac aatataaatg
     3361 ccgcactgaa gccgggatta tcggtggact gtaagataat gactttagaa aagcaagata
     3421 ttgttgtcgt tcccataggt gctttaaaga tcgacaagga tgagaatgaa tatgtgttga
     3481 agatcgataa ggaaaagggt gttatggttc gtcaggacgt gaaactgggt attatttcgg
     3541 atatgatggc tgaggttttg gacggacttg cggaaggcga cttaattgta gatgatcctc
     3601 aatcattcca caaggatgga tctaaagtaa aaatattaga ataatgaaag aggtggtttt
     3661 cagatgagct ttttagagag tattgcacag gctcttgaaa gcctcagggc aaataagtta
     3721 agatccattc ttaccatgct gggcgttatt atgggagtgt tttcgattat tacaataatg
     3781 gcaatcggaa atgctactga ggaatatatt gacagccagt ttgaaagaat cggtgccaat
     3841 ctgcttcaga ttggatataa gaatataact gttgatagag gagagtggct tcagattgaa
     3901 gatattgcga caatcaaaaa ggctgcaccg gaaataagga atgtttcaac gattattcag
     3961 aatatgggtt cattaaggat ggatacaaaa accagagatg ctttggttta cggaacaaca
     4021 gcacagtaca aggattttac tccaatggaa atggcggcag gcagattttt aagtgatttt
     4081 gatgtttctt caaggaccaa ggtggtagtg gttgatgagt attttgccaa aaggtatttc
     4141 aacaaggtgg atgtcgtagg tgagattgtc aagtttaaaa cgccttcagg atccaattat
     4201 aacctgaaga ttattggggt gactaaggca gaaaatgatt tgatggcagg tatgctggac
     4261 aatgaaaaca tgcccacata tatttatatg ccaattacca ctgttcagca aatgtataat
     4321 aacaacaaaa ggcttgactc aataatggtc agtttggata atgatgtgga ttacactgat
     4381 gttgggaaca ggatagtcag agctctagaa cttagcaagg gaaaagaaga catttatatg
     4441 tcatatagca ctcaggacac tcaaaagata ttctccagta tcattggagt aatatcagga
     4501 gttttgcttg ttatagcggt aataaccctt attgtcgggg gaataggtat tattaatatt
     4561 ttgctggtgt cggtaactga gagaataaga gaaatcggca taaggaaggc tttgggggct
     4621 caaaaaaaag acatagtcct tcagtttatt aca
//
LOCUS       DAOOJC010000163         4610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14699_length_4610_cov_3.789682, whole genome shotgun sequence.
ACCESSION   DAOOJC010000163 DAOOJC010000000
VERSION     DAOOJC010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14699_length_4610_cov_3.789682"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PLA01_05580"
     CDS             <1..617
                     /locus_tag="PLA01_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003375556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPD00804.1"
                     /translation="IVELKPDLVIASSIFQKEVLEKLEELNIKVAVLQGEESFEGAYD
                     VIGKVGILLNSKDEAKKLINDMKEKVELVKNSVKELDKPSVYYVIGYGEFGDYTAGRD
                     TFIAHIIEMAGGINVADDVDGWKYNIESLLEKDPDMLICSRYFDSKQGIMNTAGYKEL
                     TAVKNGRVFEIDNNMLDRQGPRLADGLLDMAKIIHPEAFGETES"
     gene            619..1665
                     /locus_tag="PLA01_05585"
     CDS             619..1665
                     /locus_tag="PLA01_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HPD00805.1"
                     /translation="MSFIDKKKKYRLLFIAGLLLLLFLIVISATIGAAHISLMDAFRI
                     MAKKLPFVGSLLGEWSASETHELIVLNVRLPRILAAAVIGIGLSVVGAAYQSMFVNPM
                     ADPYVLGVSSGAALGASIAIVLGAERLAGGFGLVTAAAFIFALLTVFIVFSIARTGAR
                     ISSTHLLLAGVAVSFFATSLISVLMVLNRDRITKITYWMMGSVAYITWKQVLILIPVI
                     ILGTVVICFFARDLNIIVTGEEGAKSLGVEVEAVKKVLLVVCSIVVAACVSVSGVIGF
                     VGLIIPHTIRLIAGSDNRVVLPFSAVGGGIFLIICDTIARMPTSEIPVGVLTSMFGAP
                     YFISILLRNKRKVV"
     gene            1667..2461
                     /locus_tag="PLA01_05590"
     CDS             1667..2461
                     /locus_tag="PLA01_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD00806.1"
                     /translation="MGQQSVSYPINVKNINVSFGSINALKNISLCMEKGRFYGILGPN
                     GSGKTTLLKSIAKNLKPQRGAVFINGADIGKMKAKELSKELAYMYQNTDTQCDYSVTD
                     VVLMGRNPYLRRFQTEGSMDREIAMKAMKATNIWQLKDRRINEISGGERQRAFIARAL
                     VQDTDIVLLDEPVSHLDLQHQIEVLDIARKMARKQRTVVAVLHDLNLASTYCDCLILL
                     KEGAVIAQGEPEKVLTEEIIEEVYGIKLYIVRGVNNNRPYIIPFKK"
     gene            2772..3266
                     /locus_tag="PLA01_05595"
     CDS             2772..3266
                     /locus_tag="PLA01_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPD00807.1"
                     /translation="MSENKTLKNLMAAFAGESQANRKYNAYANKAEKEGKNNIAKLFR
                     VTADAETIHALKEYELAGKIGSTEDNLKDAIAGETYEYEEMYKEFLNVAEEEGNKAAA
                     LAFKFAMKAEEVHAKLYKEALENIDSAEEVTYYLCPVCGNIEKFVPDKCSICGVPGSK
                     FIKY"
     gene            3447..3767
                     /locus_tag="PLA01_05600"
     CDS             3447..3767
                     /locus_tag="PLA01_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPD00808.1"
                     /translation="MGVIKYNKLVRDRIPEIIEKSGKKAIVEELGNQEYKKCLDIKLG
                     EELQEYLAEDSVDELADLVEVVYPILKYKGIDIGEFESIRKRKAEERGAFDKRLMLKE
                     VVEG"
     gene            3780..>4610
                     /locus_tag="PLA01_05605"
     CDS             3780..>4610
                     /locus_tag="PLA01_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPD00809.1"
                     /translation="MENEVRYTSSLASVAEDLFVEIFCDVFGPEKTQYLSVQHPFVDI
                     YGNRRFIDFALESEELRIAIEIDGEKFHNPEKVSRNKYYDDLLKQNSLIYYNWKVYRW
                     VYNQLKNQKEKVKDELLTFISEMSQFRMLEDYLPKQKGKIIELKDHQQSAIENLRKMR
                     DGGESIALLYHATGTGKTVTAVSGAKDFGERTLFLAHTRELVSQAKKTFEDIWPDKKS
                     GMYVAEQKDDSSYIVCASIQSIAQNLERFKPDDFGYIIIDECHHSVADTYRKILGYFK
                     P"
BASE COUNT         1456 a          706 c         1164 g         1284 t
ORIGIN      
        1 aaattgtgga attaaagccc gatttggtta ttgcttcttc gatttttcag aaggaggtat
       61 tggaaaagct tgaggaatta aacattaagg tagctgtttt gcagggcgaa gagagctttg
      121 aaggtgccta tgatgttatt gggaaggtag ggattttgct gaattcaaaa gacgaagcga
      181 aaaaattaat aaacgatatg aaggaaaagg tcgaacttgt aaaaaacagc gtaaaggaac
      241 tggataaacc tagtgtttac tatgttatag gttatggtga gttcggggat tatactgccg
      301 gaagagacac ctttattgcc catattatcg aaatggccgg tggaatcaat gtcgctgacg
      361 atgtggatgg ctggaagtat aatattgaaa gtcttttaga gaaggaccct gacatgctta
      421 tatgctccag gtacttcgat tcaaagcagg gcattatgaa taccgcgggt tataaagagc
      481 ttacagctgt aaaaaacgga agggtttttg aaattgacaa caatatgttg gacaggcagg
      541 gaccacggct tgccgacgga ctgcttgata tggcaaaaat aatccaccct gaagcctttg
      601 gtgaaacgga gagttaatat gtcctttatc gacaaaaaga aaaaatacag gcttttgttt
      661 atagccggtt tattgctttt gctgttttta atagtcattt cggcaacaat aggagctgcc
      721 catatatcgc tgatggatgc tttcaggata atggctaaaa agctgccctt tgttggcagc
      781 cttttggggg aatggtcagc cagcgaaacc cacgagctta ttgtacttaa tgtaaggctt
      841 ccccggattt tggctgctgc tgttatcgga ataggacttt ccgttgttgg agcagcatat
      901 cagagtatgt ttgtaaatcc catggccgat ccttatgtct tgggagtatc gtcgggtgct
      961 gcactgggag cttctattgc cattgttctg ggtgctgaac ggttggcggg aggatttgga
     1021 ctggtgacgg ccgctgcatt tatttttgca ttgctaacgg tgtttatagt tttcagtata
     1081 gcaagaaccg gagcacggat ttccagtacc caccttcttt tggcgggtgt agcggtgagt
     1141 ttttttgcca catccttgat atccgtcctc atggttttaa acagggacag gataacaaag
     1201 ataacgtact ggatgatggg gagcgtggca tacattacct ggaaacaggt tttaatattg
     1261 attcccgtaa ttatattggg cacagttgtg atttgtttct ttgcaaggga tttgaacata
     1321 atagttaccg gagaagaagg ggcaaaaagc cttggggttg aggttgaagc agtcaaaaaa
     1381 gtactgctgg tggtatgctc cattgttgtt gcagcatgtg tttccgtgag cggggttatt
     1441 ggctttgtag gccttataat tccgcataca atccggttga ttgccggctc ggacaacagg
     1501 gttgtgctgc cgttttccgc tgtgggaggc ggaatatttc tgattatatg cgacactatt
     1561 gccagaatgc cgacatcgga gatcccggtg ggagtgttga catccatgtt cggtgcaccg
     1621 tattttatct caattttgtt gagaaacaag aggaaggtgg tttgatttgg gacagcaaag
     1681 tgtatcgtat ccgattaatg ttaaaaatat taatgtgtcc tttggaagca ttaacgcatt
     1741 gaagaatata tccttgtgta tggaaaaagg caggttctat ggcattttag gaccaaatgg
     1801 ctccggtaag accactcttt taaagagcat tgccaaaaat cttaagcccc aaaggggtgc
     1861 ggtttttatc aacggtgcgg atataggtaa aatgaaggca aaggagcttt caaaggagct
     1921 tgcgtatatg tatcagaaca ctgataccca atgcgattat tctgttacgg atgtggtgct
     1981 tatgggcaga aatccgtatt tgagaagatt tcagaccgaa ggttccatgg acagggaaat
     2041 tgcaatgaag gcaatgaaag ctacaaatat ttggcagctc aaagacagga ggattaatga
     2101 aataagcggg ggagagaggc agagggcatt tattgcacgg gctctggtgc aggatacgga
     2161 catagtttta ttggacgaac cggtttccca cctggacctg cagcaccaga tcgaggtttt
     2221 ggatattgcc cgaaagatgg cacgaaagca aaggactgtt gttgcggttt tgcatgatct
     2281 caaccttgca tccacctatt gcgactgttt gattttgcta aaggaaggag ccgtcattgc
     2341 ccagggagag cccgagaagg ttctgacgga ggagattatt gaagaggtgt atggcataaa
     2401 gctatatatt gtaagagggg taaacaataa cagaccctat ataattccgt tcaaaaagta
     2461 ggttgctaca aaaatttgta aatatatcgg aaatataagg tataaataat agaaatatat
     2521 gcaacaatta acaataaggc aaactgattc aaattctgtc cctcagggga ctattttttt
     2581 tattgcatag ttttttagtt actcaatgtt aattttcttt acacttgttc cgggaaatat
     2641 atgaagggta aaaggaaggt taattaaaag atggtaaatt aatctggtta atggctttat
     2701 attgtggtat tataatttat gattatgatt tgggtattat ttaaaaataa aaatattgga
     2761 ggtagacaga aatgagtgag aacaaaacct taaaaaatct gatggcggca tttgcaggcg
     2821 aatcccaggc aaacagaaaa tataatgctt atgcaaataa ggctgaaaaa gaaggtaaaa
     2881 acaatattgc aaaattgttc agggttactg ctgatgcaga aacaattcac gcccttaagg
     2941 aatatgaact ggcaggtaaa atcggttcca ccgaagataa tttgaaggat gccatagcag
     3001 gtgaaacata cgaatatgaa gagatgtata aagaattctt aaatgtggct gaagaagaag
     3061 gaaataaagc agcagctttg gcatttaagt ttgcaatgaa ggctgaagag gttcatgcta
     3121 aattgtataa ggaagcattg gaaaatatag attctgcgga agaggtaacc tattatctgt
     3181 gtcctgtatg cggcaatata gaaaagtttg ttccggataa gtgcagtatc tgcggagtgc
     3241 ctggaagcaa attcattaaa tactaaaagt tatgccggga gagacggcat tgaggaaaac
     3301 aaatggtgct tatactgcgg tgtaggtacc attttgcata ttatgagttg atgccatggt
     3361 gttttatttt acaggaatat gctgtataat gccattatac gtgataataa acatgcagga
     3421 ttaatagatt cgggagattg agaaaaatgg gcgtaatcaa gtataataag cttgtaagag
     3481 acagaattcc ggagataatt gaaaagagcg gtaagaaggc aattgttgaa gagcttggaa
     3541 atcaagaata taagaaatgc ctggatatca aactgggtga agagctgcag gaatatctcg
     3601 cggaagacag tgtggacgaa ttagcagatt tggtggaagt tgtttatccc atattgaaat
     3661 ataaaggcat tgatattggt gagtttgaaa gtataaggaa aagaaaggcc gaagaacggg
     3721 gagcctttga taagaggctg atgcttaagg aagttgtcga aggataggag tgcacgctaa
     3781 tggaaaatga agtaagatat acttccagtt tggcaagtgt tgctgaagat ttatttgttg
     3841 aaatattctg tgatgttttc gggccggaaa aaacacaata tctttcggtt cagcaccctt
     3901 ttgtagatat atacggcaat aggagattta ttgattttgc tcttgaaagt gaagaattga
     3961 gaattgcaat agaaattgac ggagaaaaat ttcacaatcc cgaaaaggtt tccaggaata
     4021 aatactatga cgatctgctg aagcagaaca gcttgatata ttacaattgg aaagtataca
     4081 gatgggtata taaccaattg aaaaatcaaa aggaaaaggt aaaggacgag ttgttgacct
     4141 ttatcagtga aatgtcccag ttcaggatgc tggaggacta cctgccgaaa cagaagggaa
     4201 aaataattga attaaaagac caccagcaat ctgcaattga aaacctcaga aaaatgaggg
     4261 atgggggaga aagcatcgct cttttgtatc atgcaacagg tacgggaaaa accgttactg
     4321 cagtcagtgg tgcgaaggac tttggagaaa gaactctttt tctggcccat acccgtgagc
     4381 ttgtgtccca ggcaaagaaa acctttgaag atatttggcc cgataaaaaa agcggtatgt
     4441 atgtggcaga gcaaaaggat gacagttctt acatagtgtg tgcaagcata caaagcattg
     4501 cacagaatct tgaacggttc aaacccgacg attttggcta catcattatt gatgaatgcc
     4561 atcacagtgt tgcagatacg tacagaaaga tcctgggata tttcaaaccg
//
LOCUS       DAOOJC010000164         4604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14727_length_4604_cov_4.062871, whole genome shotgun sequence.
ACCESSION   DAOOJC010000164 DAOOJC010000000
VERSION     DAOOJC010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14727_length_4604_cov_4.062871"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..253)
                     /locus_tag="PLA01_05610"
                     /pseudo
     CDS             complement(1..253)
                     /locus_tag="PLA01_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(651..1664)
                     /locus_tag="PLA01_05615"
     CDS             complement(651..1664)
                     /locus_tag="PLA01_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPD00810.1"
                     /translation="MSFFRNPEIKTSLFVFCILTLLSTGLGFVFDYKVGIYTLAVCLI
                     FSLGHIIITYRRYSKISGLSREIDSILHGKDSMNLDEYSEGELAILGTEIQKLTIRLR
                     EQNGALQRDKTYLADSIADISHQIRTTLTSINILISLLSKTNLSEERRLEIVREIEML
                     LIRIDWLISALLKMSRLDAGTVKLQRKTVSVAELIKQSVAPIAIPMELREQQIEIRMK
                     GDETYEGDFAWSVEAIGNILKNCMEHMQKGGRIVIEASENPVFTEIVISDNGPGIDKE
                     DLPHLFERFYKGKNSGDQSIGIGLALARMIITSQNGTVRAGNNSDRGARFTVRFYKGE
                     TKK"
     gene            complement(1666..2337)
                     /locus_tag="PLA01_05620"
     CDS             complement(1666..2337)
                     /locus_tag="PLA01_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPD00811.1"
                     /translation="MPKILVVEDDKSIVTSLTEYLCNEGFEIDSVNGQAEAIEKISLA
                     KYDLVLLDISLCDGNGFAVCAAVKANTDTPVIFLTASDDEYSVVTGLDMGADDYIGKP
                     FRPRELLSRIRNVLRRNGKTGSVIEIDNICVDTVKGTVTKNGKEVFLSALEYRLLLVF
                     LNNKGAILSRNRLLEEIWSVAGEFVNDNTLTVYIKRLRDKIEDDPQNPTVIKTVRGLG
                     YKVGE"
     gene            complement(2510..4528)
                     /locus_tag="PLA01_05625"
     CDS             complement(2510..4528)
                     /locus_tag="PLA01_05625"
                     /inference="COORDINATES: protein motif:HMM:NF015387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPD00812.1"
                     /translation="MRIRNKLIMLLIVTLLLTCLVGMQSSAANYNVSVDISTKYQTLE
                     GFGAAIAWYNNWLTGHPNKNELYNTLFYDMGLDILRLRNQYRYNNNFAYDDAEIVKMA
                     KILNPDLKVFLASWSPPEDLKRDGVMNGGTLKKENGSFVYDKFADYWYDSLIAYKEKG
                     IVPDYISIQNEPDYEDQNWETCILKPTEDQNYPSYGKALDAVYNKIKSLPDMPKILAP
                     EAAGIGFNTVQNYTAGMDLSKVYGIAHHLYNGGDGSNPDSFNSIFKTLAETYPDKPLF
                     QTEYDYGTPFTTAQLIHNSLVVEGVSGYFYWDLIWENAQRPLVFIENPFNPNGWTTEK
                     GYKISDFYYTVQHYAKFTDPGYKRVEATCDSNDIKVTAFVSLDESKLTMIFINNASSQ
                     NTVSLDLNGFSYSESAIYRTLENGSEKFSLAGSLSGNTVTLPAKSVVTVALGFDDSAT
                     PPPLPTPTPPPTSRSAFELIQAEDYNSMYGISREVISEDGNKNISYIENDDYACYEGI
                     DFGTGATGFEARVSSNTSGGKIEIRLDGPNTQTIATISVPGTGDWNNYVDISAEVSGL
                     TGKHDLYLYFSGGGDYLFNIDSFRFTGGTVPTPTPAGKIGDLNGDDSVDSTDITLMKR
                     YLLRKISDFPVDDDLYAADVNGDDEINSTDLTLLKRYVLRKIDKFPKQ"
BASE COUNT         1309 a         1048 c          831 g         1416 t
ORIGIN      
        1 tgcgtctgaa aattgccagt ttttcatcgc tctgggcata aacatcctgc ccgtccatat
       61 acaccttgcc cccggttggc ttatccaccc cgcccaatat atgcaaaagt gtggacttgc
      121 ctgagccgga cggtccgata accgctataa actcaccttt ttgaacctaa aaggatacac
      181 cgtccagtgc cctgaccatg ttttctcccc tgccataggt ttttgtcaaa ttctcaaccc
      241 tcaatagttc cataaatcaa atccatctcc catttctagt ctatgtcata attatatcct
      301 tttaaggtga catgacagtg acttaaaaat tacaaaaatg tcacctgata aaattattat
      361 gtggggggtg agatagtgtc gcttcttaag tcgctgcttt taacatctac ccttcaatgg
      421 ttgtatatta ttttagtgaa gaaaaatgtg gaagaaacat tgtgaagcaa tattttactt
      481 cacatttgtt taattcccct tcaaccgtaa ataagcttcc agctgatttt attatacagg
      541 cagaaaaggt cttcggactt ggtgaaggcg taaaaccaca taagaacact tcaatcgtat
      601 gattcattga gttgatattg tgtaatgtat gcaacagaaa aaggttaaga ttacttttta
      661 gtttctcctt tgtaaaacct cactgtaaat cttgcacccc tgtcactgtt gtttcctgcc
      721 ctgaccgtgc cgttttgact ggttattatc atccgggcaa gagcaagtcc tatgccaata
      781 ctttgatcgc cggaattctt ccctttataa aacctctcaa aaaggtgcgg caaatcctct
      841 ttatctattc cgggtccgtt gtcgctaatg acaatctcgg taaagacagg gttttcactg
      901 gcttctatta ctattcttcc acccttttgc atgtgttcca tacagttttt taatatgttt
      961 ccaatagcct caactgacca cgcaaagtca ccctcatagg tttcgtcacc cttcattcga
     1021 atttcaattt gctgttctct aagctccatt ggtatagcta taggtgcaac agactgcttg
     1081 attaattccg caaccgaaac ggtttttcgc tggagcttga ctgtcccggc atcaagcctt
     1141 gacattttaa gaagtgccga tatcagccag tctatacgga taagcaacat ctcaatctcc
     1201 ctgacaattt ccagccttcg ttcttcagaa agatttgttt tcgataaaag ggagatcaat
     1261 atgtttattg acgtaagagt agttctaatc tgatgggata tatcagcgat ggaatccgca
     1321 aggtatgtct tgtccctttg taatgctccg ttctgttcac gcaagcgaat tgtcagcttt
     1381 tgtatctccg tccccaaaat tgcaagctca ccctcagagt attcgtccaa attcatagaa
     1441 tcctttccat gcaaaatgct gtcaatttca cggctcaagc ctgatatttt cgagtacctg
     1501 cggtatgtta taataatatg acccaagctg aatataagac acactgccaa agtatagatt
     1561 ccgactttat aatcgaaaac aaagcctaaa cccgttgaca gcaacgtcag aatacaaaaa
     1621 acaaaaaggc tggtttttat ttccggattt ctgaaaaagc tcatcctatt caccaacctt
     1681 atatccaagc ccgcggacgg ttttaataac tgtcggattc tgcgggtcat cctcgatttt
     1741 atcccgtaag cgttttatat atacagtaag ggtattgtca tttacaaact cgcctgcaac
     1801 gctccatatc tcctctaaaa gcctgtttcg cgacagaata gcccccttat tgtttaagaa
     1861 aacaagcagc agcctatact caagggccga aagaaaaacc tctttaccgt tttttgtgac
     1921 agtaccttta accgtgtcca cgcaaatatt atcaatctct ataaccgatc cggtttttcc
     1981 gttccttcta aggacattgc gtattcgcga taaaagctcc cttgggcgaa agggctttcc
     2041 tatatagtca tctgccccca tatcaagacc ggtaacaaca ctgtattcat cgtccgaagc
     2101 cgtcagaaaa ataaccggag tatcggtgtt tgctttaacg gcagcacaaa ctgcaaagcc
     2161 atttccgtca cataaggaaa tatccagaag aaccaaatca tatttggcca agcttatttt
     2221 ttcaattgcc tctgcctggc cattgactga atctatctca aacccctcat tgcacaaata
     2281 ttcagttaaa ctggtcacaa tgcttttgtc gtcttcaaca acaagaatct tcggcataca
     2341 gtttatctcc atcttattgc taaacagatt atgttaagat tatcaataca aatattaatg
     2401 tattgaaaaa ttgctacaat aaataacggc aaaaacatta tagcattttt cttaactgtt
     2461 ttccatctat acaaataggt ttgatgcata tttcaaaatt ctataaccat tattgtttcg
     2521 gaaacttatc tattttacgc agcacgtacc tttttaacag ggtgagatcc gtggagttta
     2581 tttcatcatc accgttcaca tcagcagcat ataaatcatc atccacagga aaatcactta
     2641 tctttcgaag cagatacctc ttcataagtg taatgtccgt cgaatcaaca ctgtcatctc
     2701 cgtttaaatc cccaatcttt cctgcaggcg taggtgtcgg taccgttccg ccggtaaatc
     2761 tgaatgagtc gatattaaac aggtaatctc ctcctccgct aaaatacagg tataaatcat
     2821 gcttgcccgt aagaccgctg acttcggctg atatatcaac ataattgttc caatcccctg
     2881 taccgggaac ggaaattgtt gcaattgttt gtgtattggg tccgtccagc cttatttcaa
     2941 tttttcctcc gctggtatta ctggaaaccc gtgcctcaaa tccggttgct ccggttccaa
     3001 agtctatgcc ctcatagcaa gcatagtcat cgttctcaat gtagcttatg tttttgtttc
     3061 catcctcact aataacctct ctgctaatac catacatgct gttgtaatct tcggcttgaa
     3121 taagttcaaa tgcacttctt gatgtcggag gcggtgtagg tgtcggcaac ggcggaggtg
     3181 tcgcactatc atcaaatccc agggcaaccg taaccacgga ttttgccgga agagtgacag
     3241 tattgccgga aaggcttcct gccagagaga acttctcgct tccgttttcc aaagtgcggt
     3301 aaattgcgga ttcgctatag gaaaatccat tcaaatctaa agaaacagtg ttttgggatg
     3361 aagcattatt tataaaaatc attgtcagct tgctctcgtc aagggaaaca aaagcagtga
     3421 cttttatgtc gttcgagtca caggttgctt ctaccctctt atatcccgga tcggtaaatt
     3481 tcgcataatg ttgaacagta tagtagaagt cggaaatttt gtagcctttt tcggttgtcc
     3541 agccattagg attaaaggga ttttcgatga aaaccaaggg tctctgagca ttttcccaaa
     3601 tgagatccca gtaaaaatac ccgcttaccc cttccactac aagggagtta tgaatcaact
     3661 gagcggttgt aaaaggcgta ccgtaatcat attctgtttg gaataacggc ttgtcgggat
     3721 aagtttcagc aagggttttg aaaatagaat taaaactgtc gggattgctg ccgtctccgc
     3781 cgttatacaa gtgatgtgca ataccgtaaa ccttcgaaag atccatcccg gcagtgtaat
     3841 tctgaactgt gttaaaaccg attcctgctg cttccggagc aagaatctta ggcatatccg
     3901 gcaatgattt tattttatta taaacagcat ccaaggcctt gccataggaa ggataattct
     3961 gatcctccgt aggctttaaa atacaagttt cccagttttg atcctcataa tccggctcgt
     4021 tctgaatgct gatataatcc ggtactattc ctttttcttt ataagctata agtgagtcgt
     4081 accaataatc tgcaaacttg tcataaacga aggaaccgtt ttcttttttt aaggtaccgc
     4141 cgttcataac gccgtcccgc ttaagatcct ccggtggaga ccatgatgcg agaaaaacct
     4201 taaggtccgg attaaggatt tttgccatct taacaatttc tgcatcatca taagcaaaat
     4261 tgttgttgta cctgtactgg ttcctcaaac gcagtatgtc caatcccatg tcataaaaaa
     4321 gagtgttgta aagctcgttt ttgttgggat ggcctgtcag ccagttatta taccaggcaa
     4381 tagctgcacc aaagccttcg agtgtctggt atttggtact gatatcaacc gatacgttgt
     4441 agttggctgc actcgattgc atacctacaa gacaggtcaa taaaagcgtt acaataagaa
     4501 gcataatcag tttgtttcta attctcatca aagcaccctc cctataaaat ttatattaat
     4561 agtcttgata aatcaaaacc attaaagaca caataataat caag
//
LOCUS       DAOOJC010000165         4603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14732_length_4603_cov_3.407432, whole genome shotgun sequence.
ACCESSION   DAOOJC010000165 DAOOJC010000000
VERSION     DAOOJC010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14732_length_4603_cov_3.407432"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1351)
                     /locus_tag="PLA01_05630"
     CDS             complement(131..1351)
                     /locus_tag="PLA01_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HPD00813.1"
                     /translation="MKKIMGLLKNKNGSLTLEAAISLPIFMCVFLTIAFFMRVVYIQN
                     NVQYALNGAANELATYSYLYSVSGFQELNDSISGEMNKYGERASNRTKQLLEAFDAMG
                     DNLQNAVDSVEGGLKTGDLKKLENLEELYNQSKISAGTVKNVLNDVKNDPKMEFVSIA
                     SLFLGAGYENIKGELSEPLIKFFMAKYIDNKIINSGGIPGAYIEVKNGDDPLKAFDFK
                     SRLFTDNKSIDIVVRYEIKTALPINIIPEIPMEQRVTVRGWLDGDASVLGSDEGSSDE
                     ESIWDKAPFVYGKFITEEELKKYPSRYPDSGDAYEVRSINLDCSTYKDIDKARSSLKS
                     GINTFISKTMNNKAINSRTFIIVIPEGTLTEEVKTMFEEMKSEALKSDPPVKVVYKEG
                     YGRQKLGEKTDDQV"
     gene            complement(1398..1904)
                     /locus_tag="PLA01_05635"
     CDS             complement(1398..1904)
                     /locus_tag="PLA01_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00814.1"
                     /translation="MSKLKPVFIVFLCLLLLPACGKKEPDRSNAQAFLEYAIHAADAT
                     NFLETGNYAGVMLNLKREYKDDIVNPYTKGDTLAVFNRTRAMFKNNKTSLTKYFSVDC
                     LVIDDYVTTLKDLDTSEVVYCLNAESKGMVLAFFLMDGIYIYEVDENGEKKNEIKHFF
                     EKKSKETD"
     gene            complement(2009..>4603)
                     /locus_tag="PLA01_05640"
     CDS             complement(2009..>4603)
                     /locus_tag="PLA01_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014522643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5702 domain-containing protein"
                     /protein_id="HPD00815.1"
                     /translation="KDVEDKIEEYEDEIEELKDEIERKKKEAQENEKDKEGSEIGDSA
                     NASEGGMTEEPAKADTSEETLRISELTALIEDLEDSISGLKSDIENNENDLKTKKTQI
                     TQIETALLNYLDKTVNAAQKSKSSLIGIRDESKQTVKMIDGIGEELKGETNEFSNTVR
                     VDLGSKKEKISTEDLNPKIEQVEHNISILKDLQSCIHNRKLDELGLSDFGGQVPDIDE
                     IRDRLNIGLIMDKIREYNGKANGIPIDYYEDKGIISDGPKDGEKDPRDAIKDFIKNGG
                     PKDQKEIKENEKKMPEDVPSQKGYEGTSLEEIARDKEYIDSVLKIIASQDYYTAQRLS
                     GDIKDLTNIDINDASFSGSDTSFSKNGLSIVSQFSDMFVDGLYNLRDEIFIDEYIMGN
                     FTNYTTDMEKDLDLRGRLMKERPVFFDANNADVEYVLWGSENESTNLLAVKAQITLVR
                     FALNTIAIYTDPTKYNTALEVATVVAGWTAIGVPIVHTLVMLAWAMAESLFDTYFLLK
                     GESIPIFKTRNTWITDLDGLTKTITDEFINQTKEHAKLAIDNAIDYTENEVESFLDKA
                     STTLWDYIDSKIDLLVDKAFVNIENPFSEETYSADNIFEDVEDTLVVKVDGDLGEIMK
                     KIGSDAHDLLVERMGEVEKDTLESFFPDEIRGVDYTDVFYQYRGRNFKEAIAQLSYIV
                     SQGEALPGLSVEETSKKINSLIFNTLQDAKNKVKFSIKEKIMGYKSGLIESFKESLQK
                     TADKGKEEVNKLIDSLGNTEDSKAMKTNLKASFLSMKYTDYLRLFLLLVDSDKKVERV
                     ADLIQMNMRKASGKDSFKMSDCSTYMRVESEVSIKYLFLSKPFIPEALRTEDGKRIKL
                     DTVLYKGY"
BASE COUNT         1247 a         1089 c          661 g         1606 t
ORIGIN      
        1 taaaagttgt atttttacat atcatttacc cacacaaaat agcgaagcac cgaaataata
       61 tattattctt ttacttcatt cattattttt cgaaaatgca gcttgcacct acttatcaaa
      121 caatacagac tcatacctga tcatctgttt tctctccaag cttttgtctt ccatatcctt
      181 ctttatatac aacttttaca ggaggatcac tctttaacgc ctcgctcttc atttcttcaa
      241 acattgtttt tacctcttct gtcaaagtgc cttccggtat tactattatg aaggttcttg
      301 aattgattgc cttgttattc attgtcttgg atataaatgt attaataccc gatttcaaag
      361 aacttcttgc cttatcaata tctttataag tgctgcaatc cagattaata ctccttacct
      421 cataggcatc gccggaatcg ggatatcttg acggatactt cttcagctcc tcctcagtta
      481 tgaatttacc ataaacaaaa ggtgctttat cccagatgga ttcctcatcg gatgacccct
      541 catcggaacc taataccgat gcatccccat ccagccatcc ccttaccgtt accctctgct
      601 ccataggaat ttcgggaata atgtttatgg gaagtgccgt ctttatttca tatcgtacca
      661 caatatctat ggatttatta tcagtgaaca acctgctttt aaaatcaaaa gcctttaaag
      721 ggtcatcccc attttttacc tcaatataag cccctgggat gccaccgcta ttaataattt
      781 tattatctat gtattttgcc ataaagaact ttataagggg ttctgaaagc tcacctttta
      841 tattctcgta tcccgcaccg agaaataatg atgcaatgct gacaaactcc atttttgggt
      901 catttttaac gtcatttaat acgtttttaa cggtacccgc agatatcttg ctttgattat
      961 acagttcctc cagattttct aacttcttta gatctccggt tttaaggcct ccttccaccg
     1021 aatccacagc attttgaagg ttgtcaccca tggcatcaaa agcctctaat agctgttttg
     1081 tgcgattgga agccctttct ccatatttat tcatttcacc cgaaatagaa tcgttcaatt
     1141 cctggaaacc ggaaactgaa tatagatagc tataggtcgc aagctcgttg gcagcaccgt
     1201 ttaaagcata ctgaacatta ttctgtatat acaccactct cataaaaaat gctatggtta
     1261 aaaaaacgca catgaatatt ggcaacgaaa tagctgcttc caaggttaat gatccatttt
     1321 tgtttttcag taatcccatt atttttttca ctgctctcac ccactgcaaa ctaactgaat
     1381 ttacatccaa ttacggttta atccgtttct ttgcttttct tttcaaaaaa atgctttatt
     1441 tcatttttct tttcaccgtt ttcgtcaact tcataaatat atatgccatc catcaaaaag
     1501 aacgccaaca ccattccctt gctttcggca ttcaggcaat aaacaacttc ggaggtatcc
     1561 aagtctttca gggtagtcac ataatcgtct atcacaaggc aatccacact aaaatacttg
     1621 gttaatgaag ttttattgtt tttaaacata gccctggttc tgttgaaaac agccagtgta
     1681 tcacctttag tgtaaggatt tacaatatca tccttgtatt cgcgttttaa gttcagcata
     1741 acccctgcat agttccctgt ttcaagaaaa ttcgttgcat ccgcagcatg tattgcatac
     1801 tccaaaaaag cctgggcatt gctcctgtca ggttctttct tcccgcatgc cggtaacaac
     1861 agcaaacaca aaaaaacaat aaacaccggt ttcaatttac tcatctttaa gagttccttt
     1921 caacaaagtc atttataatg taaaacacaa tttacaagtc accttcacct ttttagaatc
     1981 tttcaacaac gttttctata aaaacttgct aatacccctt atacagtaca gtatcaagct
     2041 taatccgttt tccgtcctcg gttctcaaag cttccggaat gaatggtttc gataaaaaca
     2101 aatactttat ggaaacctcc gactcaaccc gcatgtatgt actgcagtcc gacattttaa
     2161 aagaatcctt tcccgatgct ttcctcatat tcatctgaat taagtccgct accctttcaa
     2221 ccttcttatc actgtcaaca agaagcaaaa acagcctcaa atagtcggta tacttcattg
     2281 aaaggaaaga agcctttagg ttggtcttca ttgcttttga atcctcagta tttcccaaag
     2341 aatctataag cttgttaacc tcctcttttc ccttatctgc cgtcttttga agactttctt
     2401 taaacgattc tatcagcccg gacttgtatc ccattatttt ctccttgata ctgaacttga
     2461 ccttattctt tgcatcctga agggtattaa aaatcaggct gtttattttc ttactggtct
     2521 cttcaacact taaacccggc agagcttctc cctgcgaaac tatgtaagat aattgggcaa
     2581 tagcttcttt aaaattcctt cccctgtatt ggtagaaaac atcggtatag tcaacgcctc
     2641 tgatttcatc cggaaagaag ctttccaatg tgtccttttc aacttccccc attctctcaa
     2701 ccaacaagtc atgagcatcg cttccgattt ttttcattat ttctcccaaa tcaccgtcaa
     2761 cctttactac taaagtgtcc tccacatctt caaaaatgtt atccgccgaa taagtttctt
     2821 cgctaaaggg attctcaatg ttgacaaaag ccttgtccac caacaaatct attttagaat
     2881 caatataatc ccaaagggtc gtactggcct tatccaagaa actctcgact tcattttcgg
     2941 tatagtcaat tgcattatcg attgctaatt ttgcatgttc cttggtttga ttgataaatt
     3001 catctgtaat ggttttagtt agaccgtcaa gatcggttat ccatgtgttt cttgtcttaa
     3061 atatcggtat actttctcct tttaaaagaa agtatgtatc aaacaaggat tccgccatag
     3121 cccaggccag cattaccaat gtatgtacaa taggcacacc tatagcagtc caccccgcaa
     3181 caaccgttgc aacctctagt gcagtattat atttcgttgg atcggtatat atcgctatgg
     3241 tattcaaagc aaacctcaca agggttatct gtgccttaac ggccaacaag tttgtggact
     3301 cattctcact tccccacaaa acatattcga catctgcatt atttgcatca aaaaacacag
     3361 gtctttcttt cattagccgc ccacgcaaat ccaaatcctt ttccatatcc gttgtataat
     3421 tggtaaaatt gcccattatg tactcgtcta tgaaaatttc atccctcaaa ttataaagcc
     3481 catctacaaa catatcggaa aattgggaaa caatacttaa tccgtttttc gaaaagctgg
     3541 tatccgatcc tgaaaaagaa gcatcattaa tgtcaatatt tgttaagtct tttatatctc
     3601 ccgataatct ctgagcagtg taataatcct gtgatgcaat aatcttaaga accgagtcaa
     3661 tgtattcctt gtcccgggca atttcttcaa gggaggtccc ctcatatcct ttttgggaag
     3721 ggacatcttc aggcattttc ttttcgttct cttttatttc cttttggtct ttcggtccac
     3781 cgttcttaat aaagtcctta atggcatccc tcgggtcctt ctcgccatcc ttcggaccgt
     3841 cagagatgat tcctttatcc tcatagtaat caatgggaat gccattagcc ttaccgttgt
     3901 attctcttat tttgtccatt atcaaaccaa tattaagcct gtcacgtatt tcatcaatat
     3961 ccggaacctg tccgccaaaa tcgcttaatc caagctcatc taatttccta ttatgtatac
     4021 aactctgcaa atccttcaat atactgatat tatgctcaac ttgttcgatt ttgggattca
     4081 agtcttctgt cgatattttt tcttttttgc ttccgaggtc cacccttacg gtgtttgaga
     4141 actcgttggt ttcacctttg agttcttcgc cgattccgtc aatcattttt acggtctgtt
     4201 ttgattcatc tcttataccg ataagtgaac tcttagactt ttgggctgca ttaacagtct
     4261 tgtccaaata atttaacaat gcggtttcta tctgtgtgat ctgtgtcttt ttcgttttta
     4321 ggtcattttc attattttca atatccgatt ttaaaccgga tattgaatct tccaaatctt
     4381 caattaatgc cgtaagctca ctgattctga gggtttcttc agaagtatcc gctttcgcag
     4441 gctcctccgt catcccgcct tctgaagcat tggcactgtc tccaatttcc gaaccttctt
     4501 tatctttttc attttcctgc gcttcttttt tctttctttc gatttcgtcc ttcagctcct
     4561 caatttcatc ttcgtattct tcaattttat cttcaacgtc ctt
//
LOCUS       DAOOJC010000166         4600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14749_length_4600_cov_2.801320, whole genome shotgun sequence.
ACCESSION   DAOOJC010000166 DAOOJC010000000
VERSION     DAOOJC010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14749_length_4600_cov_2.801320"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="PLA01_05645"
     CDS             complement(<1..1243)
                     /locus_tag="PLA01_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPD00816.1"
                     /translation="MKRIGLYVLIAALLAGIIPISVLAEEPKFNYVDAFAKSILFYEA
                     NWCGPDAGNNRIKWRGPCHIEDGKDVGLDLTGGFHDCGDHVKFGLPQCASASTLAWAY
                     YEFSDVFIEKGQDEYMLNILKHFCDYFMKCYPEKNKFYYQIGDGDVDHQYWGPPELQT
                     YDRPAYYVATPENPGSDVAGDTAAALALMYLNYKDRDLEYAEKCLTYAKDIYEFGMTY
                     RGNSKGQSYYLPRDYLDELMWGSVWLYVATGEQEYMDNVDKLMVEKRIGDDAGNSFND
                     NWTQCWDYVLTGVFIKLATLSAKPIYKEIAEDHLDYWQNRLKSSPGGIKFLDSWGVCK
                     YPAAESMVQLVYYKYTGDKSCLDFAKSQIDYILGDNPNKMSYVVGFGDNYPKFPHHRA
                     ASGRLEGQPADETKNDPQRHIL"
     gene            complement(1352..4237)
                     /locus_tag="PLA01_05650"
     CDS             complement(1352..4237)
                     /locus_tag="PLA01_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPD00817.1"
                     /translation="MINAPKKRILKQKNRCKRLITALIVATQLLTTSILYAEAPPETF
                     TPAENWEAYDYFNFAEALQKSLYFYDAEKCGAEAGYDQGGRLEWRGSCHVVDERIPIS
                     NTSLSEAFLEKYRDIIDPDGDGTVDVHGGFHDAGDHVRFGLPQSYSAGTLGWGFYEFR
                     ESFKAIGEEEHMIEILRYFTDTFLRCSFMDEEGNLVAFCYMVGEGDEDHCYWGPPELY
                     PEEFLKSRPADFATFDAPGSDVCASTAAALCTSYLNFMDEDPEYAEKCLTVAIAMYDF
                     AVKYRGLHDGDGYYTSDYDEDELAWAAVWLYECTGDMKYINDITAVDETGNYVGYMKR
                     IIPETFKQNIWYNSWVHCWDAVWGGTFLKLNELFPENELFDFIARWNVEFLSGEKCPH
                     EDPNDHNYYKTSPAGYTMINGWGSARYNTAAQLSALVYMKNNPDRTDFGEWAKGAMEY
                     LMGRNPMGYSYIVGYGYEKGLPFVKHPHHRAAHGSKTNSMNDPEEHRHILWGALAGGP
                     DITDYHIDSTTEYAYNEVTIDYNAAFVGALAGLYNYYGQGHEPIPNFPPLEPRTDDYF
                     CEAKIVRETKDSTQVLLRIHNESSQPPHYETGMMARYFFNISELIENDQSIEDVIFAI
                     EYDEQISMQQDPITYRGPFKWDDAGTYYFEFDWSGSKIYGDRELQISFRVKQDLNYMT
                     HWDSSNDYSRKGLTSEFEVCKNVPVYLDGVKAYGEEPPKLSPTPTPTKDPDETPDINA
                     SISVSYKCGAKDGTKNTIRASINIKNTGTTPVNLSDIKVRYWFTSDGNERNIFVCDYA
                     ACGTENVKGTVKKIENPVPGADSYCEISFSEDAGKIAPGGSTGTIPFRIDGAADYDQT
                     DDYSYDSNMSDDLGDNTKITAYIKDKLKYGVEPVTTIDILLGDLNYDGEVNSTDCTIL
                     KRYILRKIDIGSDPNFLTAADVNRDGSVDSTDLTLIKRYILRKIKSF"
BASE COUNT         1267 a         1048 c          803 g         1482 t
ORIGIN      
        1 ataaaatatg cctttgcgga tcgttctttg tttcatcggc cggctgtcct tcaagtcttc
       61 cgcttgcagc tctgtgatgc gggaatttgg gataattgtc tccaaaacca accacatagg
      121 acatcttatt gggattatct ccaaggatat agtcaatttg gctctttgca aaatcaaggc
      181 agctcttgtc cccggtatac ttatagtaca ccagttgaac catgctctct gcagcaggat
      241 atttgcaaac accccagcta tccaggaact ttatgcctcc gggagaagat tttagcctgt
      301 tctgccagta atccaaatgg tcttcggcaa tttctttata tataggctta gcggagagag
      361 tggcaagctt tataaataca ccggtcaaaa catagtccca gcattgcgtc caattgtcgt
      421 taaaggaatt gccggcatca tcgccaatcc ttttttcaac catcagctta tccacattgt
      481 ccatgtattc ttgctctcct gtggcaacat aaagccatac ggagccccac attagttcat
      541 caagataatc cctgggaaga tagtaacttt gccctttact atttcctcta taggtcatac
      601 cgaactcata aatatccttt gcataagtaa ggcatttttc tgcatattcc aagtccctgt
      661 ctttgtaatt taaatacata agtgccaatg ccgctgccgt atccccggca acatccgaac
      721 ccggattttc cggtgtggcc acataatatg caggtctgtc ataggtctga agctcaggtg
      781 gtccccagta ctggtggtcc acatctccgt cacctatttg atagtaaaac ttattctttt
      841 ccggatagca tttcatgaag taatcgcaaa aatgctttaa aatgtttagc atatactcat
      901 cttgtccctt ttctataaac acgtctgaga attcatagta agcccaggca agtgttgatg
      961 ctgaagcaca ttgaggcaat ccgaacttaa cgtggtctcc gcagtcatgg aagcctcccg
     1021 tcaaatcgag tcccacatcc ttgccgtcct caatatgaca cggaccacgc cattttatcc
     1081 tgttgtttcc tgcgtcagga ccgcaccagt tggcctcgta aaacagaatt gatttggcaa
     1141 acgcatctac ataattaaat tttggttctt cggccagaac cgatattgga attattcctg
     1201 ccagaagagc ggcaatcaaa acatataaac ctattctttt cattatttat tcccccaatt
     1261 aacattctca gtgtaaattc cattacactt ttaatcagta aattccctat aaatgacccc
     1321 tctttatcta taccttaatc tgcctgtttt tctaaaaaga ttttatttta cgaaggatat
     1381 aacgttttat taatgtaaga tccgttgaat cgacacttcc atccctgtta acatctgcgg
     1441 ctgttaaaaa gttaggatcg gatcctatgt caattttcct aagtatatac ctttttaaaa
     1501 ttgtacagtc tgtagagtta acttcaccgt catagttcag gtcacctaat aaaatatcaa
     1561 ttgttgtaac aggctcaacc ccatatttga gtttatcttt tatataagca gtaatcttgg
     1621 tgttatcacc taaatcatcc gacatattgg aatcatagga ataatcatct gtctggtcat
     1681 aatcagctgc accatcaatt ctgaaaggta ttgttcctgt gcttcctcca ggtgcaatct
     1741 tacctgcgtc ctcgctaaag gagatttcac aatacgaatc ggcacccggg acggggtttt
     1801 ctatcttttt gacagtaccc tttacatttt ccgttccaca agccgcataa tcgcacacaa
     1861 agatatttcg ctcatttccg tcacttgtaa accagtatcg aaccttgata tccgataaat
     1921 tcacgggagt agttccggta ttcttaatat ttattgaagc tcttatagta tttttcgtac
     1981 catctttagc tccgcacttg tatgatacac tgattgaagc attaatatcc ggagtttcat
     2041 caggatcctt tgttggagtc ggtgtcggag agagttttgg cggttcttcc ccgtaagctt
     2101 ttacaccatc taaatatacg ggcacattct tgcatacttc aaattcactt gtaaggcctt
     2161 ttctgctata gtcattgctg gaatcccaat gagtcatata attcaaatcc tgtttaactc
     2221 taaaggaaat ctgaagctcc ctgtctccat aaattttact tccgctccag tcaaattcaa
     2281 aatagtatgt tcctgcatca tcccatttaa aaggtcctct gtatgtaatg ggatcctgct
     2341 gcatggaaat ctgttcatca tattcaatag caaatatcac atcttctatg ctttgatcgt
     2401 tttcaataag ctcacttata ttgaagaagt atcttgccat cattcctgtc tcataatgag
     2461 gaggctggct ggattcatta tgaattctta aaagaacttg cgtgctgtct ttagtttcac
     2521 ggacaatttt tgcttcacag aaataatcgt cggttctcgg ttctaagggc gggaaattgg
     2581 gaatgggttc atgcccctgt ccgtaataat tatacagtcc tgccagtgct cctacaaagg
     2641 cagcattata gtcgattgta acctcattat aagcatactc ggtagttgaa tcaatgtgat
     2701 aatcagttat atcggggcct cctgcaagtg ctccccacaa aatgtgacgg tgctcttcag
     2761 ggtcgttcat actgtttgtc tttgagccgt gagccgctct gtggtgcgga tgcttgacaa
     2821 aaggcagacc tttctcatag ccgtaaccga ctatgtacga ataacccata gggtttcttc
     2881 ccataaggta ttccattgca ccctttgccc actcaccgaa atctgtccta tccggattgt
     2941 ttttcatgta aacaagagca gataattgtg cagctgtatt gtatctggca gatccccaac
     3001 cgtttatcat tgtgtaccct gcaggggatg ttttataata attatgatca ttgggatcct
     3061 cgtgcgggca tttttcgccg gacaaaaact caacattcca tcttgcgata aagtcgaaca
     3121 attcattttc agggaacagc tcgttcagtt ttaagaaggt tcctccccat acggcatccc
     3181 aacaatgaac ccaagaatta taccagatat tctgtttaaa ggtttcgggt attatcctct
     3241 tcatatatcc tacataattg ccggtctcat caacagcagt tatgtcattt atgtatttca
     3301 tgtctccggt gcactcatag agccaaacag cagcccatgc cagttcatct tcgtcataat
     3361 ccgaggtgta gtaaccgtca ccgtcatgca aacctctgta cttgactgca aaatcataca
     3421 ttgctatggc aactgtaaga catttttcag catattccgg atcttcatcc atgaaattca
     3481 aatatgatgt gcaaagtgcc gctgccgtac tagcacaaac atcgcttccg ggagcatcaa
     3541 aagttgcaaa atccgccggc ctgcttttta agaattcttc agggtataat tccggaggcc
     3601 cccaatagca atggtcttcg tctccttcac ctaccatgta acaaaaagca acaagattac
     3661 cttcctcgtc cataaatgaa caacgtagaa atgtatctgt aaagtatctt aaaatctcaa
     3721 tcatgtgctc ttcttccccg attgccttaa aggattctct gaattcatag aatccccacc
     3781 ccaaagtacc tgctgaatag ctttgaggaa gaccgaaacg gacatggtcg cctgcatcat
     3841 ggaaccctcc gtgtacgtct acagttccgt ctccatcagg gtcaattata tctctgtact
     3901 tttctagaaa agcttcagaa agggatgtgt tggaaatagg aattctctca tccactacat
     3961 gacaggatcc tctccactca agccttccgc cttggtcgta acctgcctct gctccgcatt
     4021 tttcagcgtc ataaaaatac agcgatttct gcaaggcttc ggcaaagtta aagtaatcat
     4081 aagcctccca gttttcagca ggtgtaaagg tttcaggcgg tgcttctgca tataatatac
     4141 tcgtcgtcag tagctgtgtt gcaacaatga gtgctgttat aagcctctta cacctgtttt
     4201 tttgttttaa aattcttttt ttaggtgcat taatcatttc acaaattccc ccccatgtac
     4261 aatatttata tatatttata aaagtaagaa tagatatact ataataaaac gtatattatc
     4321 ataaattcga cataaatatt ccgattatca actattatta taatatattc gaagaggaaa
     4381 aagaatttct gtgggtattt gataaaaaat tatgaatata tatactttag attagataac
     4441 taaccaaaaa atgtatattt tgcgggataa tttttggaac ctttgtttta tgtcccctgt
     4501 tttatgcttc aaaaaaagat tctgtttcca tatcaccact tattgttatg tattctttct
     4561 tttaaattga tttccttttg ttctgctatt tttgcatctt
//
LOCUS       DAOOJC010000167         4597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14763_length_4597_cov_3.537649, whole genome shotgun sequence.
ACCESSION   DAOOJC010000167 DAOOJC010000000
VERSION     DAOOJC010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14763_length_4597_cov_3.537649"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLA01_05655"
     CDS             <1..219
                     /locus_tag="PLA01_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPD00818.1"
                     /translation="APTVTAVNSIGSGDAMVAGFAVGLVENKSPEECLKLAMACGVSN
                     TQFVEIGVVDKDNVEVQLDNIEIEILGS"
     gene            596..2752
                     /locus_tag="PLA01_05660"
     CDS             596..2752
                     /locus_tag="PLA01_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPD00819.1"
                     /translation="MSDIISTLIKEFNLKLFQVQNTVKLIDDGNTIPFIARYRKEMTG
                     ELNDQVLRELYERLIYLRNLEARRQEVKHLIEEQGKLTEEITMSLMKAVTLQEIEDIY
                     RPFRPKRRTKATVAREKGLEPLAILLFSQDKKEGSVTDFAQPFVNPEKGVNSVEEALN
                     GAMDIIAEDISDDAEIRKDIRNMFMKQGIVVSKAKTEEDSVYRMYYDFSEPVPKIAGH
                     RVLAINRGEKEEFLKVKIDVPSERIIEYLKIRLIKKPRSITSEYVERALADSYERLIS
                     PSVEREVRNALTEMAQEKAMKVFSTNLKNLLLQPPVKGKTVLGLDPAYRTGCKIAVVD
                     DTGKVLDTTVIYPTPPQNKVEESKKIIKYLIEKHNVDIISIGNGTASKESEIFVADLL
                     KEIDRKVYYMVVSESGASVYSASKLGAEEFPNFDVALRSAVSIARRLQDPLAELVKID
                     PKAIGVGQYQHDMNQKVLGETLGGVVEDCVNSVGVDLNTASPSLLSYVSGINSVIAKN
                     IVEFREANGRFTERKELKKVKKLGEKAYEQCAGFLRIPEGKNILDNTSVHPESYDAAK
                     SLLDILGYNLEDVKEKSLSDLKKQVEEKGITNIADRLGIGVPTLRDIINELLKPGRDP
                     RDELPKPMLLTDVLNIEDLRPGMVLSGTVRNVADFGAFIDIGVHQDGLVHISEMSDKY
                     VRSPMDVVSVGDIVKVKILDVDVARKRISMTMREID"
     gene            2813..3679
                     /locus_tag="PLA01_05665"
     CDS             2813..3679
                     /locus_tag="PLA01_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00820.1"
                     /translation="MIRVVGSVRKLVKRPDIVLAFSLATFVFCFIEYILVFPVITGIS
                     SIGGGNLMDNIMHFIQFTIGLFLNPRYLLYGLGGVLFAGLMLGFVSSGCLYMLNNYLA
                     KRKKTKLEFFTGVKKHFLKLSIVSFAQVLSSILFSVFMMVVSVPAVAVTRSFLGGNTD
                     LLALTVLFDIITLLVLFFGFMFFRIYMFSWFPAVLNFTDRHFSIGKYAADTYFWSIVM
                     RLMFLDVVFILFEYAMVYFNSAIPMDGAISVLRIVLLLFANWIFKTLFVIVLAVVFFS
                     RFLAFKEQISQE"
     gene            complement(3757..4461)
                     /gene="sleB"
                     /locus_tag="PLA01_05670"
     CDS             complement(3757..4461)
                     /gene="sleB"
                     /locus_tag="PLA01_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic enzyme"
                     /protein_id="HPD00821.1"
                     /translation="MHKRIIFLTVLAIAIVTLLGTSIMFRDEISDLYGYSRAALSRYG
                     STGQEVKDIQYKLAIWKYYNGKIDGIYGYKTYSAVRKFQIKNGLKVDGVAGPETLAAL
                     GLPTGTAQTAAAQPTGNKDLNLLAHLVYGEARGEPYMGQVAVAAVVLNRTRDSRFPKT
                     IAGVIYQPGAFDAVADGQINLAPDATAYKAARDALNGWDPSGGAIYYYNPNTATSKWI
                     WSRPIVKVIGKHNFAK"
BASE COUNT         1400 a          765 c         1071 g         1361 t
ORIGIN      
        1 gcacccactg taactgctgt taattccata ggctccggag atgcgatggt tgccggcttt
       61 gccgtgggac ttgtcgaaaa caaatcgccg gaagagtgcc ttaagttggc aatggcttgt
      121 ggtgtgagca atacacaatt tgttgaaatt ggtgtggttg ataaagataa tgttgaggtg
      181 cagttagaca atattgaaat agaaatactc ggctcataaa caaagaaatc tcttaaccga
      241 aggaaaacgt ccaaattcag gtaaacagct aataatgcaa tcttaaatgg ctttggatgg
      301 aaactgtata aagaaataac cgtattttta aaaaacagat ataaaacaga tataattcat
      361 attaagatat gggttatatc tgttttattg ttagtgggat ttcacgaagt gaaatgacca
      421 atttaaagat gccaactcaa cgctaaatcg ctcttgattg aagcattggt ttaactatat
      481 aataaattag taataaagaa ttaatctcaa tcataaataa attaatcagc aaaggtttgt
      541 ggggaaaagt actgtcggtt tttaatatgg atttaatact atatggagga atgaaatgtc
      601 ggatataatt tcaacactga taaaagaatt taacctgaag ctttttcaag tacaaaacac
      661 tgtaaaactt attgatgacg ggaacaccat accgtttatt gccagataca gaaaggaaat
      721 gacaggcgaa ctgaatgacc aggtactaag agagctttat gagagactca tatatttacg
      781 aaacctcgag gcaaggaggc aagaggtaaa acatctcatt gaagagcagg ggaagcttac
      841 agaagaaatt accatgagcc ttatgaaggc tgttaccctt caggagattg aagacatata
      901 caggcctttc agaccaaaga gaagaacgaa ggcaaccgtt gcaagagaaa aaggcctcga
      961 acctctggca atacttcttt tcagtcagga caagaaagaa ggcagtgtaa cggattttgc
     1021 acaacccttt gtcaatccgg aaaaaggggt taattcggtt gaagaggcat taaatggggc
     1081 aatggatata atagccgagg atatatcgga cgatgcggaa attagaaaag acataagaaa
     1141 tatgttcatg aaacagggca ttgttgtgtc aaaggcaaaa acggaagaag attcggtcta
     1201 cagaatgtat tatgattttt ccgagccggt accaaagata gccgggcata gagtgcttgc
     1261 aataaacagg ggagagaagg aggaatttct aaaggttaaa attgacgttc cttccgaaag
     1321 aataattgaa tatctaaaaa tccggctgat aaaaaaaccc cggtcaataa cctcggaata
     1381 tgttgagaga gcactggcag attcatacga gaggttaata tccccatctg ttgagaggga
     1441 agtcaggaat gcacttacag aaatggcaca ggagaaagca atgaaggttt tttccaccaa
     1501 tcttaaaaac cttttgcttc agccccctgt aaagggaaag actgttttgg ggcttgaccc
     1561 ggcatacagg acggggtgca agattgcggt tgtggatgat accgggaagg tgttggatac
     1621 caccgttatt taccccacac ctccgcagaa taaggttgag gaatcgaaaa agattataaa
     1681 gtatctcata gaaaagcaca atgttgatat aatttcaata ggcaacggaa cagcttcgaa
     1741 agaatcggag atttttgttg ccgatctctt gaaggagatt gaccggaagg tatattatat
     1801 ggtggtaagc gaatcgggtg cgtcggttta ttctgcttca aagcttggtg cagaagaatt
     1861 ccccaacttt gacgtggcac tgagaagtgc cgtatccata gcaagaaggc ttcaagaccc
     1921 attggcggag ctggttaaaa ttgatcctaa ggcgatagga gtgggacagt accagcacga
     1981 tatgaaccaa aaggttttag gagagacttt aggcggtgtg gtggaagact gtgtaaacag
     2041 tgttggggtt gatcttaata cggcatcacc ttcgctttta tcctatgttt ccggaataaa
     2101 ctccgtaata gccaaaaata ttgtggaatt cagagaagcc aacggcagat ttacagaaag
     2161 gaaggagctt aaaaaagtaa aaaaacttgg ggaaaaggct tatgaacagt gtgccggctt
     2221 tctcagaata ccggaaggga aaaatattct tgataatact tcggtacatc ctgagtctta
     2281 tgatgcggca aaaagccttt tggatatttt gggatacaac cttgaggatg tgaaggagaa
     2341 aagcctttcg gaccttaaga aacaagttga ggaaaaaggg attacgaata ttgccgaccg
     2401 gctggggata ggggtaccta ctcttcggga tataattaat gaactgttaa agcccgggcg
     2461 ggatccgagg gatgagcttc ccaagcctat gcttttgact gatgtgctca atatagagga
     2521 tttgagaccg ggtatggtgt tatcgggtac tgtcagaaat gttgccgatt ttggtgcatt
     2581 tatcgatata ggagttcacc aggacggact ggttcatata tctgaaatga gcgataaata
     2641 tgtaagaagc ccaatggatg ttgtgtcggt gggagatatt gtaaaggtaa aaattctcga
     2701 tgtggatgtg gcaagaaaaa gaatttcaat gaccatgagg gaaattgatt gattaaattg
     2761 tcaataatta tacttgagct gtagtgataa agttttgaag gaaggttgca acatgattag
     2821 agtggtgggc agtgtcagaa agcttgtaaa aagaccagat atagttctgg cttttagtct
     2881 ggctacattt gttttttgct ttattgaata tatattggta tttcctgtaa ttaccggaat
     2941 atcctctatt ggcggcggaa atctaatgga taacatcatg catttcattc aatttacaat
     3001 aggccttttt ctaaatccca gatatctgct gtatggcctg ggaggagtct tgtttgctgg
     3061 tttgatgctg ggatttgttt cgtcgggatg tttatatatg ttaaataatt atcttgcgaa
     3121 aaggaagaaa accaagttgg agttttttac gggcgtgaag aagcattttt taaagctttc
     3181 aatagtatct tttgcacagg ttctgtccag tattttattt tctgtgttta tgatggtggt
     3241 atcggtacct gctgtagccg ttaccagatc atttcttggc ggaaataccg atcttctggc
     3301 actaaccgtt ttgtttgata taatcacttt gctggtgctg ttcttcgggt ttatgttttt
     3361 taggatatac atgttttcct ggtttccggc agtattaaat tttactgaca gacatttctc
     3421 cattggcaaa tacgcggcag atacctactt ttggagcatt gttatgcggc ttatgtttct
     3481 ggatgttgtt tttatattgt ttgagtatgc aatggtatat ttcaattcgg caattccaat
     3541 ggatggagca ataagcgttt taaggattgt attattgctt tttgcaaact ggattttcaa
     3601 aactctgttt gttattgtat tggcagtagt attcttttca aggtttctgg cgtttaaaga
     3661 gcaaataagc caagaatgag tcttggctga cggttctttg cctcaatgag tcaaaagaac
     3721 cgcttaccga aacataaacc tttccgaaag actcagtcat tttgcaaagt tgtgtttgcc
     3781 tatgactttt acgattggtc tggaccaaat ccatttgctg gttgccgtat tgggattata
     3841 atagtatatg gctccccctg aggggtccca tccgtttagg gcatctcttg cagccttgta
     3901 agcggtggca tccggagcaa ggttgatttg tccgtcagct acggcatcga aggctccggg
     3961 ttgatagata accccagcta tggttttggg aaatctgctg tccctcgttc tgttgagaac
     4021 cactgccgca actgctacct gacccatata aggttcaccg cgagcttctc cgtaaactaa
     4081 gtgtgccaaa aggttcaggt ctttattgcc tgttggctga gctgcggctg tttgagctgt
     4141 tccggtgggg agaccaagag ctgccaatgt ttcaggtcct gcaaccccat ctacctttaa
     4201 accgtttttg atttgaaatt tgcgaactgc cgaataggtt ttatagccat atatgccatc
     4261 aattttgcca ttgtaatact tccatattgc cagcttgtac tgaatatctt taacttcctg
     4321 tccggtagag ccataacggg ataatgcagc ccggctgtat ccgtataggt cggatatttc
     4381 atccctgaac ataattgatg tacccagaag tgttactatg gctatagcca gtacagttaa
     4441 aaaaataatt cttttatgca aagcaattct ccttttcaaa aaaatattaa taaatatttt
     4501 ttatcagaca aagttatttt gtctattttt gattcaaatt attattagtg gaataaatta
     4561 atatttttat aaaggatttt gtgtaaaaat ggcgaat
//
LOCUS       DAOOJC010000168         4578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14824_length_4578_cov_3.428477, whole genome shotgun sequence.
ACCESSION   DAOOJC010000168 DAOOJC010000000
VERSION     DAOOJC010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14824_length_4578_cov_3.428477"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            487..1620
                     /locus_tag="PLA01_05675"
     CDS             487..1620
                     /locus_tag="PLA01_05675"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPD00822.1"
                     /translation="MEDRKRVLVIFGGQSSEHEVSRISATSILKNINKEKYEVLTMGI
                     TKDGKWLPYNGDIDKIPSGEWEQIALSENVKSIGTKNSLLDAMVVCNDTDGNIDKSGK
                     AQVSGKKIDVVFPVLHGCNGEDGTIQGLFELASVPYVGCGVLGSALGMDKIYAKIMFE
                     KAGIPQADYLYFTRKEISRDVDNVVSRIEEKFAYPIFVKPSNAGSSVGVVKAHDKKEL
                     EEALNFAARYDRKVLVEEYINGREVECAVLGNDDPEASTVGEVIPGNEFYDYRAKYID
                     NSSKIKIPADLPEETIEKIRGYAVKAFKALDCCGLARVDFFVHKESGRVYINEINTMP
                     GFTSISMYPMLWEASGVPYGELIERLINLAVERYEDNSREIEG"
     gene            1729..2535
                     /gene="murI"
                     /locus_tag="PLA01_05680"
     CDS             1729..2535
                     /gene="murI"
                     /locus_tag="PLA01_05680"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPD00823.1"
                     /translation="MDNRPIGVFDSGLGGLTVLKEIRNLLPTESIVYFGDSGRAPYGT
                     KSKETVIKYTFQNIRFLLNQDIKMIVIACNTASACSIDLVKHSFDIPVIEVVGPGAVS
                     AVRETVNKKVGVIGTVATVNSGVYEKAISRLDPSIEIFQKACPMFVPLVEEGWWENDI
                     AQRIAEEYLLSLKEKGIDSLVLGCTHYPLLHKTISKVMGEGVRLVSSALEVAKVVKEV
                     INKNNAARDEKIGPVYRFYTSDSVEKFESLGNSILNEKIHSAEKIDIERY"
     gene            2647..3114
                     /locus_tag="PLA01_05685"
     CDS             2647..3114
                     /locus_tag="PLA01_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HPD00824.1"
                     /translation="MNKNVIISVKGIQTSANNDKNILELVTEGKYYKKGNAYYVTYKE
                     SEVTGMEGTTTTLKISDGVVTLMRFGSVNTQFIFEQGQKHMSYYDTQYGTFTMMVTAR
                     LVSIDVDDSGGEIKVDYKLEIDNNKSGENDLHMFIREVGQLNDKCCGDYKVSN"
     gene            3077..>4578
                     /gene="argS"
                     /locus_tag="PLA01_05690"
     CDS             3077..>4578
                     /gene="argS"
                     /locus_tag="PLA01_05690"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPD00825.1"
                     /translation="MTNVVETIKYQIEGIIKNSVQRASENGEIPELPVEELTIEVPKD
                     AGHGDFSTNIAMQAAKVAKKPPRQVADIIIKNMDLNNTYIDRVETAGPGFINFFLNND
                     WLYDALKFIQTKGEDYGRIDLGKGQRVMVEFVSANPTGPLHMGNARGGALGDCIASVL
                     ERAGYDVTREYYVNDAGNQIEKFGISLEARYIQLLKGEDAIEFPEDGYQGEDIIERVK
                     EYIEENGDKLLNVDSEERRKELVKYVLPKNVEAIRNGLESYGIKFDVWFSEQSLYDSG
                     EVQETLDYLEKNGYTTKKDGAVWFKATELGADKDEVLVRNNGIPTYFASDIAYHRNKF
                     LKRGFDRVINLLGADHHGHAGRMKCAVKALGIDPDKLDVVLFQLVRLYRNGEIARMSK
                     RTGKAISLTDLLEEVGRDAARFFFNTKASGNHLDFDLDLAVEESKDNPVFYVQYAHAR
                     ICSMLSLLESEGFKVPDINSVQLSLLEAPEEIELMKKLAAYPEEIRISAQT"
BASE COUNT         1446 a          689 c         1173 g         1270 t
ORIGIN      
        1 ccactaaatg tgaagaaaaa ataatataca cctgtttaat gcacccgtgg acatcaccct
       61 tcttttatgg tataataaaa gtggtagtat atttgtttta ttcctttcgt tattgtgttt
      121 aggtctatat caggtagtgt aaaggtaatc tttgcaagtc gtttaaaggc tgtattgacg
      181 ctttctcgtg ttaattgtat tctgtaggtg tttgttttcg caaatataat tattgatatt
      241 tatggatatt taagtaacat gtatattttg acgataatct tgaagttgcc gtacttaaat
      301 agtaaaatga gaaatatata tttactgtat agtttgtgcc tttaaacagc taattttaat
      361 tatatttttg gatagggact tttgggggca caagcttaat aatgtattta taattataaa
      421 ataccggaaa ccgggtatgt tagagaacat cagggttttg tatcaaataa taaggggaga
      481 gaacctatgg aagacaggaa aagagtactg gttatattcg gcggacagtc gtcggaacat
      541 gaggtttcac gtatatcagc tacatccata ctaaagaaca taaacaaaga aaagtatgaa
      601 gtattaacga tgggaattac aaaagacggg aaatggcttc cttataatgg agacattgat
      661 aaaattcctt ccggtgagtg ggaacaaatt gcattaagcg aaaacgtaaa gagtatcggt
      721 accaaaaaca gtctgttgga tgcgatggtt gtttgtaatg ataccgatgg aaatattgat
      781 aaatcgggaa aagctcaggt tagcgggaaa aagattgatg tggttttccc ggtactccat
      841 ggctgcaatg gcgaagatgg cactattcag ggactttttg agcttgcttc ggtgccttat
      901 gtgggctgcg gtgttttggg ttctgcgttg ggaatggata agatatatgc caagataatg
      961 tttgaaaaag cagggatacc tcaggcggac tacctttatt ttacaagaaa agaaataagc
     1021 cgggatgttg acaatgttgt ttcgagaata gaagaaaagt ttgcataccc gatatttgtt
     1081 aagccttcaa atgccggttc ttcagtgggt gtagtgaagg ctcacgacaa gaaggaattg
     1141 gaagaggcat taaatttcgc tgcccggtac gacagaaaag tactggttga agagtatatc
     1201 aacggcaggg aagtggaatg tgctgttttg ggaaatgatg acccagaggc atccacagtg
     1261 ggtgaagtaa ttccgggaaa tgagttttat gattacaggg caaaatatat tgacaacagc
     1321 tcaaaaataa agatacctgc tgatttgcct gaagagacta ttgagaaaat cagggggtat
     1381 gcagtaaagg cctttaaggc tctggactgc tgtggcttgg cgagggttga ctttttcgtt
     1441 cataaggaaa gcggcagggt ttatataaat gaaatcaaca ctatgcccgg ttttacaagc
     1501 atcagtatgt atcccatgct ctgggaggca tccggggtac cttatggaga gcttattgaa
     1561 aggttgataa atcttgcagt tgagagatat gaagataatt cgagggaaat tgaaggatag
     1621 agattttggt tttgcaaagc agtaatcagg atttcgtact tggcgaaggt gcgttataga
     1681 ttaatataat tatgcagttt agtcgtatta tatttggggg aaaatactat ggataacaga
     1741 ccgattggcg tttttgattc aggattgggc ggacttaccg ttttaaagga gattagaaat
     1801 ttgctgccga cagagagcat agtttatttt ggagacagcg gaagagcacc atatggaacg
     1861 aaatcaaagg aaaccgttat caaatataca tttcagaaca tacggtttct tttgaatcaa
     1921 gatataaaga tgattgtaat tgcatgcaac accgcaagtg cttgcagtat tgatttggtg
     1981 aagcacagct ttgacattcc ggtgatagag gtggtagggc ccggagcggt gagtgctgtg
     2041 agggaaacag tgaacaagaa ggttggggta ataggaacag tagcaacggt aaacagcgga
     2101 gtttacgaaa aggcgataag ccgcttggat ccttcgatag agatattcca gaaggcatgt
     2161 cccatgtttg taccccttgt tgaggagggc tggtgggaga atgatattgc acaaagaatt
     2221 gctgaggaat atttgctctc attgaaggag aagggtatag acagccttgt tttagggtgt
     2281 acccactatc ctcttttgca taaaaccatc tcaaaggtga tgggagaggg cgtcaggttg
     2341 gttagttcgg cattggaggt tgccaaagtt gttaaggagg tgataaataa gaataacgcc
     2401 gcaagggatg aaaagatcgg acctgtgtac aggttttaca ccagtgacag tgtcgaaaag
     2461 tttgaatctc taggaaattc cattttaaac gaaaaaatac attctgctga aaaaattgac
     2521 attgagagat attagtaaca cttataaccg gttgaatcat ggaagagctt tatgtactaa
     2581 gaaagcagga aggtatttaa attattagta ggggcagtat gcccttataa aaatggaggc
     2641 ggtcttatga ataagaatgt tattatatcg gtaaaaggga ttcaaacttc tgcaaacaat
     2701 gataagaata tattggaact ggtgacggaa ggtaagtatt ataaaaaggg caatgcttat
     2761 tatgtgacct ataaagaaag tgaagtaaca ggcatggaag gtactacaac cactttaaag
     2821 atttctgacg gagtagtgac tctaatgaga tttggttcgg ttaacacgca gttcattttt
     2881 gagcagggac aaaaacacat gtcatattac gatactcagt acggaacttt cacaatgatg
     2941 gttactgccc gcttagtgtc aatagacgtt gacgacagtg gcggagaaat aaaggttgat
     3001 tataagcttg aaatagataa taataaaagt ggtgaaaatg atttgcatat gtttattaga
     3061 gaggtagggc agttgaatga caaatgttgt ggagactata aagtatcaaa ttgaggggat
     3121 tattaaaaac tcagtacaaa gggcatcgga gaacggagaa attccggagc ttccggtgga
     3181 agaacttact attgaagtcc caaaggatgc cggacatggc gatttttcaa ccaatattgc
     3241 catgcaggcg gcaaaagttg caaaaaaacc acccagacag gttgcggaca ttattattaa
     3301 aaatatggat ttgaataata catatatcga cagggttgag acggccggac ctggatttat
     3361 taattttttc cttaacaatg actggctgta tgatgccttg aaatttattc aaactaaagg
     3421 agaggactat ggcagaatag atttgggcaa gggacaaaga gtaatggttg agtttgtcag
     3481 tgccaatccc accggtcctc tccatatggg caatgcccgg ggtggtgcgt tgggtgactg
     3541 tattgccagc gttttggaga gggccggcta tgatgttacc agggaatact atgtaaatga
     3601 tgccggcaac cagattgaga agtttggtat ttctcttgaa gccagatata ttcagctttt
     3661 aaagggtgag gatgcaatag aatttcccga ggacggctat caaggtgagg acattattga
     3721 gcgggttaag gagtatattg aagaaaacgg ggacaagctt cttaatgtgg acagtgagga
     3781 aagacgcaag gaacttgtta agtatgttct tcccaaaaat gtcgaagcaa tccgaaatgg
     3841 gcttgaaagc tacggaatca agttcgacgt atggttttcc gaacaatccc tgtatgatag
     3901 cggtgaagtt caggagaccc tggattatct tgagaaaaac ggttatacaa ccaaaaagga
     3961 cggggctgta tggtttaagg ccacggagtt gggagccgac aaggacgagg tattggtaag
     4021 gaataacggt attcctacat actttgcttc cgatattgca tatcacagga acaaattttt
     4081 gaaacgcggt tttgaccggg taataaacct tttaggtgcc gaccaccacg gacatgccgg
     4141 caggatgaaa tgtgccgtaa aagctctggg aattgacccg gacaagcttg atgttgtttt
     4201 gttccagttg gtcagacttt acagaaacgg cgagatagcc aggatgtcca aaagaactgg
     4261 taaggcaata tcgttgacgg atttattgga agaggtcgga agggatgccg caagattttt
     4321 ctttaacacc aaggcatcgg gcaatcacct tgattttgat ctggatttgg cagtggagga
     4381 gtctaaggac aaccctgtat tttatgttca gtatgcccat gcaagaatat gcagcatgct
     4441 gagtttattg gaaagtgaag ggttcaaagt accggatata aactccgtgc aattgtcttt
     4501 actggaggct cccgaagaga ttgaacttat gaaaaagctg gcggcctatc cggaggaaat
     4561 aaggatttct gcccagac
//
LOCUS       DAOOJC010000169         4576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14829_length_4576_cov_3.825923, whole genome shotgun sequence.
ACCESSION   DAOOJC010000169 DAOOJC010000000
VERSION     DAOOJC010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14829_length_4576_cov_3.825923"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="PLA01_05695"
     CDS             complement(<1..1583)
                     /locus_tag="PLA01_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD00826.1"
                     /translation="MWYDNFLSIYKAGISHEFVLYFNIRDVVDNYRNIERYLYEEFIK
                     QRNFSIVAFYDISKGLTFLDAGMEKEFNKITANVAVGLKHALPSKLFPYIDIALKNTK
                     MMLFIDHTEKMIPSGDVASMSLEERMALIWLCEWSNNAKISTVGSTIVMLANTIAEIN
                     KEVFRSSYRVEPILIELPDEEARKEYINYLIKDNNIKMDISADEFAKLSSGLSKKAIK
                     DIKLKAEAENVAVSFEFIKEKKHSVLQKEYGDVLEFIYPEISFEDIGGMDRAKKYLLK
                     NIVEPIKKGDLRRVPMGILLCGPSGTGKTLLVNALAKSSGFNCVKIDMSRILGQYVGE
                     SEKNFKKCLLGAQSQQPVIVFVDEIDTAFRRGDNTDSGVSRNIFSEFLQFTSNTNNRG
                     KVVFIAATNRPDLLDAALKRAGRFDKKIPILLPEESERAEIFKIMIKKYGFETDIEDF
                     APFAAKTANYTGAEIETVVRKAYEIANEDDVDGCVLTVDILEEAISRCKPSTQQVEFM
                     TKLAIKECDDKDLLPERYR"
     gene            1834..2040
                     /locus_tag="PLA01_05700"
     CDS             1834..2040
                     /locus_tag="PLA01_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HPD00827.1"
                     /translation="METATFNVPTLSCSACSRKIEEGVKEIKGIMNVSSDLKSQHISV
                     DYNPSEITPQEIKKHISAMGYEII"
     gene            complement(2154..2834)
                     /locus_tag="PLA01_05705"
     CDS             complement(2154..2834)
                     /locus_tag="PLA01_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPD00828.1"
                     /translation="MAGKLSKVNLNDYKPLREVVFNTLREAIISGELKPGERLMEVKL
                     AEKMGVSRTPVREAIRKLELEGLVSMLPRKGAHVAELSAKDIMDVLEVRATLDGMATS
                     LAAERITDEEIKDLSHIQVQFESYTYKENLQGSIKKDVEFHELIYRASRNERLIQISS
                     NLREQIQRFRVIYMKDYSNPKDLIKEHQCILEAISRHDVEGAKRYAQIHIDNQEKSII
                     KALKDMDK"
     gene            complement(2841..3695)
                     /gene="ispE"
                     /locus_tag="PLA01_05710"
     CDS             complement(2841..3695)
                     /gene="ispE"
                     /locus_tag="PLA01_05710"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPD00829.1"
                     /translation="MSLKAYAKINLSIDITGKRPDGYHDVKMIMQTIALHDIVTLEVR
                     DSGIEIECNRPGIPTGADNIAYKAASLLMKEYNIDRGVCINITKNIPAAAGLAGGSAD
                     AAAVLKGMNSLFSLDLGEDELMAAGKQVGADVPFCIKGGTMLAQGIGEVLTELDSFSK
                     VDIVLVKPGIDVSTAWVYKNLDLCRVSFRPDTNTLIKAISNKDRVFVAQNMKNVLESV
                     TIEEYKVIKDIKEELIRLGALGSMMSGSGPSVFGIFEDRAKAEYAYERLKGEEWDCFL
                     TETLCEER"
     gene            complement(4054..>4576)
                     /locus_tag="PLA01_05715"
     CDS             complement(4054..>4576)
                     /locus_tag="PLA01_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPD00830.1"
                     /translation="FNVLCKPSLFEYSCKNGYVDLQGAVKSSILYVANNTEQPVFSYN
                     SELPFSQRIEVDNIKPGARCDVDMDIEHCNYSMLSANEVEIRIVLNINVRVLDPVDIS
                     VIADINESPVEERSLKKYPSITIYFAQPGDNLWRIAKKYCTTVEDICRANEINQDDAI
                     EIGQQIIVPRKIS"
BASE COUNT         1267 a         1087 c          715 g         1507 t
ORIGIN      
        1 tcccggtatc tttccggtaa aaggtccttg tcgtcacact ctttaattgc cagcttcgtc
       61 ataaactcca cttgctgtgt actgggcttg catcttgaaa tggcctcttc taaaatatcc
      121 accgtcaaaa cgcatccgtc cacatcatcc tcatttgcaa tttcgtaggc ctttcgcacg
      181 acagtttcaa tttccgcccc ggtatagttc gcggttttag ccgcaaaagg tgcaaaatcc
      241 tctatatctg tctcaaaacc gtatttttta atcattatct taaatatttc cgctctttca
      301 ctttcttcag gaagcaatat cggtattttc ttatcaaacc ttcccgcccg tttaagtgcc
      361 gcatctaaaa ggtcaggcct gtttgtagcc gcgataaaaa caactttccc cctattattt
      421 gtattactgg tgaactgtaa aaattcactg aaaatgtttc tgctcactcc actgtctgtg
      481 ttatcccctc ttctgaaagc tgtgtctatc tcgtcaacaa acactataac aggctgttgc
      541 gactgtgcac ctaaaagaca ctttttaaaa ttcttctcac tctcacccac atattgacct
      601 aaaatccgcg acatatcaat ctttacacag ttaaaaccgc tggattttgc aagggcattg
      661 acaagaagag ttttgcccgt tcccgacggt ccgcataaaa gtattcccat cggcaccctt
      721 ctcaaatctc ccttttttat cggttctaca atattcttaa ggaggtattt tttggcccta
      781 tccattcctc ctatatcctc aaagcttatt tcaggatata taaactccaa aacatctcca
      841 tattcttttt ggagcactga gtgtttcttc tcctttataa attcaaagct tacagccaca
      901 ttctcagctt cagcctttag ctttatgtct ttaatagcct tcttgctaag tcccgatgac
      961 agcttggcaa actcgtctgc actgatgtcc atttttatat tattgtcttt tataaggtag
     1021 tttatatact cctttcgtgc ttcttcatcg ggaagttcaa taagaatcgg ctctaccctg
     1081 tatgaagatc tgaacacctc tttgtttatt tcggcaatag tgtttgccag cataactatc
     1141 gtacttccta ctgtggatat tttggcattg ttagaccatt cacacagcca tataagtgcc
     1201 attctttctt caagggacat ggaagccaca tcgccgcttg gaatcatctt ctccgtatgg
     1261 tcaataaaaa gcatcatctt tgtattcttt aatgcaatgt ctatatacgg aaacaacttt
     1321 gacggaaggg cgtgctttaa tcccaccgcc acatttgctg ttattttatt aaactccttt
     1381 tccatccccg catccaagaa tgtcaaacct ttggaaatat catagaaggc tacgatggaa
     1441 aagttcctct gtttaataaa ctcttcgtag aggtatcttt caatattcct gtagttatcc
     1501 acaacatccc ttatattaaa gtataataca aactcatggg atatccccgc tttatatatg
     1561 cttaagaaat tgtcatacca catttacggc tttttgcctc ccgatacata aatttttgtt
     1621 tcatcgatat aattgcaata ctatataaac tgcaaaacat atatcttaac atgttatttc
     1681 tattttatca caagatacat ccaaatcaat agaaatatat acacaaaggt acgggaatca
     1741 aaatgaatca atttaagaat aataattttt acttttgaaa tgctaatata tagtcgaagg
     1801 tttataaaat gaaaccggtt aaggaggaat acaatggaaa cagcaacttt taatgttcca
     1861 acactatcat gcagtgcctg ctcacgcaag attgaggaag gtgtaaaaga aataaagggt
     1921 attatgaatg tatcttccga cctcaaaagc cagcatatta gcgtggatta caatccttcg
     1981 gagattaccc cgcaggaaat aaagaagcac atatccgcga tgggttatga aataatttag
     2041 aaaatgcacc gaaacagaat ttttaagtac ggcacctttg gtataatcta ctcatatacc
     2101 agggtgcctc ccgaaaacat ctcatgtccg acacgaattg tgattcccat aggctactta
     2161 tccatgtcct ttaacgcctt tataattgat ttctcctgat tgtctatatg gatttgggca
     2221 tacctcttgg ccccttccac atcatgcctt gaaatagctt caagaatgca ttgatgctcc
     2281 tttataagat cctttgggtt gctgtaatcc ttcatatata tcactctgaa tctttgtatc
     2341 tgctccctga gattactgga aatctggata agcctttcgt tcctggaagc cctgtaaatc
     2401 aactcgtgaa actccacatc ctttttaatg gacccctgca aattttcctt ataggtatag
     2461 ctctcaaact gtacctgtat atggctcaaa tcctttatct cttcatcggt tattctttca
     2521 gctgcaagac tggtggccat tccgtcgaga gtagccctga cctccaaaac atccataata
     2581 tctttagccg ataactctgc aacatgtgct cctttacgcg gaagcatgct aaccagccct
     2641 tcaagctcca gcttcctgat tgcttccctt acaggggtgc ggctgactcc cattttttct
     2701 gccagcttaa cctccatcaa cctttccccc ggttttaact ctccggaaat aattgcttcc
     2761 ctaagagtat taaaaactac ttccctcaag ggtttataat cattgagatt tacctttgaa
     2821 agctttccag ccattttgtc tcatctctcc tcacataacg tttctgttaa aaaacaatcc
     2881 cattcttcgc cctttaatct ctcataggca tactctgcct ttgcacggtc ttcaaatatc
     2941 ccaaacacgg aaggaccgct cccgctcatc atgcttccca aagccccaag ccttatgagc
     3001 tcttctttta tatcctttat aactttgtat tcttctattg taacagactc cagcacgttt
     3061 ttcatgtttt gtgccacaaa taccctatcc ttgttggaaa tcgcttttat cagcgtgttt
     3121 gtgtccggtc tgaaagaaac cctgcacagg tccagatttt tgtataccca tgctgtagat
     3181 acatcaattc caggcttaac caatacaata tcaacctttg aaaaggaatc caattcggtg
     3241 agcacttccc ctatgccctg tgcaagcatg gttcctccct ttatacagaa aggaacatcg
     3301 gcaccaactt gtttgccggc agccatgagt tcatcttcac caagatccaa tgaaaacagt
     3361 gaattcatac ctttgagcac agcagcagca tcggcacttc ctcccgccaa acctgcagca
     3421 gcaggaatgt tctttgtgat gttaatgcaa acacccctgt cgatattata ctccttcata
     3481 aggaggcttg ctgctttata tgcaatatta tcggctccag tcggtatacc gggcctattg
     3541 cactcaatct caataccact gtccctgact tcaagggtaa ctatgtcatg cagggctatg
     3601 gtctgcataa tcatctttac atcatgataa ccatccggtc tctttccggt tatgtcaata
     3661 gatagattta ttttagcata tgccttcaag ctgatcgatt ccattttaca cccttttctt
     3721 aataatttgc cgattatttt atgaaatatt tttttattaa ttatatacaa tacaaaattc
     3781 tttttcaaca atggtaaatt acaaaaagtc tggaggttgc cggtctaaac tttgataaac
     3841 tggttaactc aacagatatt acaatattga acacggagta aatacctggg cagaatttac
     3901 ttgccttcaa tgattgtttg cattatacag gataaactaa aggacttgaa aataaaaatt
     3961 caagtccttt agtaatcaaa caggctctgt accttcgaat cttaagtttt gaatattttt
     4021 ctttacattt tcaattcacc cgtaattatg tacttaactt attttccttg gaactataat
     4081 ttgctgcccg atttcaattg catcatcctg attaatctca ttagccctgc atatgtcttc
     4141 caccgttgtg caatatttct ttgcaattct ccacaggtta tctccaggtt gtgcaaagta
     4201 tattgtaata ctgggatact tcttgaggct tctttcttca acagggcttt catttatgtc
     4261 tgcaataacg gaaatgtcca ccggatctaa aactcggaca tttatgttta acacaattct
     4321 tatctcaact tcatttgccg aaagcatgct gtagttgcaa tgctcaatat ccatatccac
     4381 gtcgcacctt gcaccgggtt ttatgttgtc tacctctatt ctctgactga agggtaattc
     4441 actgttataa gaaaacaccg gctgttcagt gttgtttgcc acataaagga tactgctttt
     4501 aacagctccc tgcaaatcca cataaccgtt tttgcagcta tattcaaaca ggctgggctt
     4561 gcacagaaca ttaaac
//
LOCUS       DAOOJC010000170         4571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14852_length_4571_cov_3.791851, whole genome shotgun sequence.
ACCESSION   DAOOJC010000170 DAOOJC010000000
VERSION     DAOOJC010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14852_length_4571_cov_3.791851"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2691
                     /locus_tag="PLA01_05720"
     CDS             <1..2691
                     /locus_tag="PLA01_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPD00831.1"
                     /translation="GWTRKSYWLFKDRQWDLYSGIQDTKDELSVLKWRYHVRQLQRDL
                     KYFSVTRMMYLPGSDKYNSITENIDKLWIDGIFDEWTQRAVLQFKDAAKAGARFDPIR
                     RELVFPYIDNPTFKGKLDGVVDEETKNEIERWHDYVHNILNIDSVVVEDCSIPSVKLT
                     GLKIVAPMLACSALVKEQGNVNILLACPIKSDNNEITADDIRENLNCFMFLHEPADKN
                     RPFLCDDYVPNKCREDKYTVVEVAEADESFGLFYKGTKNPEVFKTLAQNIDTWYEEEV
                     IYSVTENAENKEIVRRKIWDIYDKKYPGLLEKLGEEGLKLWKVVIKLSGNVKPGFHLL
                     KIKDGNDLKPHPVRVFPSEKEEYKVIHLTDLHIASRYDELFDCLGDSLPHYNNPNDRL
                     REFIEKARHIMPDLIIITGDAVDNANNHRPYDTQKGKHVFKSVVDMDSNWRRLHYILT
                     TEPGIDVPVYISLGNHDFKHNPSSIQHRCKDLNISQEEAENYTYDTVDSNSTGAVSKW
                     RFSKSLGDTLYADENAAQYYFENFCPFTDFSFNIENLNFILMNSGPDKKIFISDYSSA
                     QQWDETWAYVREILSGDAPAPTSQGFGERQLSWLKKVIEDKGNYTNIFCMHSNVINPS
                     VTGLMLKENALQDDLDDDRQTPGEMLVGMGGIIRLEGWGCEDIIDREGWLTESARNKL
                     RNTAGDPPRRWMPMSAGDLEYIREKIKIIDNIIEDLKMGKELNHSELYVLPEEKYPQA
                     MRDSWYPALMEELESLKNKLNSLLEIDYSVSSIDKGRSELLKLFENGSLKIALTGHLH
                     KNMEFRCERKDGRVKWYSGNYSDYDECLDYFNQQGDGLVLSTACSGFLGTEDTYDPKN
                     NEYGKKHEKAWYRMLSISSNGQIRGFKKIPLGDKNDAGLY"
     gene            2733..3428
                     /locus_tag="PLA01_05725"
     CDS             2733..3428
                     /locus_tag="PLA01_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00832.1"
                     /translation="MGKDLDKSSVDIDNIDISVPTVDLEKDSKPLDRAVIKINPIEYR
                     IKKFGLFKYTAALRNLTIAYNGDIQYKIKECIDYYKNNTDNKEALIYKSVGSSAASMT
                     TCALISDQDGNFFVCSLNEDIWFDDVKTPDDYSSIYAGLTVVETGGRKYLPNMDNEEG
                     YYKVNYIVTSDLYVLVAVPNRLDLTEGNANYYALRGNSAYSQDIVTSLIFEAKSGELE
                     KYVTSKFYKGEVL"
     gene            3425..>4571
                     /locus_tag="PLA01_05730"
     CDS             3425..>4571
                     /locus_tag="PLA01_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00833.1"
                     /translation="MKTVRIPDDALRQYYNDSAEFSKIIEYFKKSGIKHEVNIDALTG
                     YKFIQYGDNDRSMYIVKADEGVYLPESRYKFFPKELLSEKRVITIGFENNHIKPGQSV
                     TLYIDTPRSIYNVKVFLENIKTKERKQLYNIIPEMLYRGKINVYWIADNDIKLGEYRA
                     VLIWSEYESRRKESVSSVSVLKVITDTGIRLGKYFLSNGSDHIYIYTRKVLKEELYHD
                     EKSKEEAFKKLVLFIMENSKREESYAVIETAASGSNKRNMLRLMTINHNFSIKDLHKD
                     GNVKNLLPPSPQELAELESENRKIESIIIGNYVQLCLGLDEIGKYVYKCADIISENYN
                     LDDEVFFKFKFSNKIKEYSFSIESIKGAHTNRYILPFEFSQYIQIKLL"
BASE COUNT         1571 a          695 c         1040 g         1265 t
ORIGIN      
        1 ggatggacaa gaaaaagcta ttggcttttt aaggaccggc aatgggattt gtattccggt
       61 attcaggata cgaaggatga actatcggtg ttgaagtggc ggtaccatgt aaggcagctt
      121 caaagagatt taaagtactt tagtgttaca agaatgatgt atttgccggg aagcgacaaa
      181 tacaattcga taactgaaaa tattgataaa ctatggattg acggtatttt tgatgaatgg
      241 acacagcgtg cggtgcttca atttaaagat gctgcaaaag ctggggcaag gtttgatccc
      301 ataagacggg agctggtttt cccgtatata gacaatccca ccttcaaagg taaactggat
      361 ggggttgtgg atgaggaaac aaagaatgaa attgaaagat ggcatgatta tgtacataat
      421 atattaaata tagacagtgt tgttgttgag gactgctcta ttccgtctgt caaattgacg
      481 gggttaaaaa tagtagctcc aatgttagct tgctcagccc tggtaaaaga acaggggaat
      541 gtaaacattt tgctggcgtg tcccattaaa tctgacaaca acgagattac cgctgatgat
      601 ataagggaaa atctcaattg ctttatgttc ttgcatgagc ctgcggataa aaacaggcct
      661 tttctatgcg atgattatgt tccgaataaa tgcagggaag ataaatatac ggttgttgaa
      721 gtagcagaag cagatgagtc ttttggtctg ttttataaag gtaccaaaaa tcctgaggtt
      781 tttaagactt tagcacaaaa tatagatact tggtatgagg aagaggtaat ttattcggtt
      841 actgagaatg cggaaaataa agaaattgta aggagaaaaa tatgggatat ttatgataaa
      901 aaatatccgg ggcttcttga aaagctagga gaagaagggc tgaagctgtg gaaggtggtt
      961 ataaaattaa gcggaaatgt caagcccggt ttccatctgt tgaaaataaa ggacggaaat
     1021 gatttgaagc cacatcctgt aagggttttt ccttctgaaa aagaagaata taaagtaata
     1081 catcttaccg atttacatat agcttcaagg tatgacgaat tattcgattg ccttggcgac
     1141 agtttgccgc attacaataa ccctaacgac aggctcaggg aatttattga aaaagccagg
     1201 catataatgc ccgatttaat tataattacc ggggacgcag tagacaatgc aaataaccac
     1261 agaccctatg atactcagaa aggaaaacat gttttcaaat ctgtggtgga catggacagc
     1321 aactggagaa ggctgcatta tatacttacg acagaaccgg ggatcgatgt accggtatac
     1381 atatccctgg gcaatcatga ttttaaacac aatccttctt caatacagca tcgctgcaag
     1441 gatttgaaca tatcacagga agaggctgaa aactatactt atgatacagt agattccaat
     1501 agcactgggg cagtttctaa gtggcggttt tcaaaaagcc tcggagatac attatatgcc
     1561 gatgaaaatg cagcacagta ttattttgag aatttctgcc cttttacgga cttttctttt
     1621 aatattgaaa accttaattt cattttgatg aattcaggac ccgataaaaa aatctttata
     1681 agtgattatt ctagtgcaca gcaatgggat gaaacctggg cttatgtccg cgagatttta
     1741 tcgggagatg ctcccgcacc gacatcacaa gggtttggcg aacggcagct ttcatggcta
     1801 aaaaaggtga tagaagataa gggaaattat accaatattt tttgcatgca cagcaatgta
     1861 atcaatccct cggtgacggg acttatgctg aaggaaaatg ctctgcagga tgatttggat
     1921 gatgacagac agacaccggg ggaaatgctc gtcgggatgg gtggaattat acggcttgaa
     1981 ggatggggct gtgaagatat aattgacagg gaagggtggc taaccgagtc tgcccgaaac
     2041 aaactgagaa atacggcagg tgatccgcca agacggtgga tgcctatgtc agcaggagat
     2101 ttggagtata tccgtgaaaa aataaaaatt attgataata ttatcgaaga ccttaaaatg
     2161 gggaaagaac tgaaccacag tgagttgtac gtattgccgg aagaaaagta tccccaggca
     2221 atgagggatt cgtggtatcc agctctgatg gaggagcttg aaagccttaa gaacaagctt
     2281 aattcccttt tggagattga ctacagtgtc agttccatag ataagggcag aagtgagcta
     2341 ttaaaattat ttgaaaacgg aagccttaag attgccctta ccggacattt gcataaaaac
     2401 atggaattta gatgtgaaag aaaagacggc agagttaagt ggtattcggg aaactattcc
     2461 gattatgatg aatgcctgga ctactttaat cagcagggtg acggactggt actttccact
     2521 gcttgttccg gatttctcgg aaccgaggat acctatgacc caaaaaataa tgaatacggc
     2581 aagaagcatg aaaaagcttg gtacaggatg ctgtcaataa gcagcaacgg gcaaataaga
     2641 gggtttaaaa aaataccctt aggggataaa aatgatgcgg gactgtatta acaagaggat
     2701 atcacttctg ttatttgatg ggggttataa ttatgggcaa ggatttggat aaaagcagtg
     2761 tagacattga taacatcgat ataagcgttc caacggtaga tttggaaaaa gactcaaagc
     2821 ctctggacag ggcagttata aaaataaatc cgatagaata ccgtattaaa aagtttgggc
     2881 tgttcaaata cactgctgcg ttaagaaatc ttaccatcgc atacaatggg gatattcaat
     2941 acaaaattaa agaatgcatt gattattata aaaacaatac cgacaataaa gaagctttga
     3001 tttacaagtc cgtaggttct tctgcagctt caatgaccac ttgtgcactt atttcggatc
     3061 aggatggcaa cttctttgtc tgttctttaa atgaggacat atggtttgac gatgtaaaaa
     3121 ccccggatga ttattccagt atttacgcag gattaaccgt tgtcgagacc ggaggaagaa
     3181 aatacttgcc caatatggat aatgaagagg ggtattataa agtaaattat attgttacat
     3241 ccgacttata tgtgcttgta gcggttccca accgcctgga cttgacagaa ggcaatgcaa
     3301 actattatgc attaagggga aacagtgcct attctcagga tattgtcaca tcgcttatat
     3361 ttgaagcgaa atccggagaa cttgaaaagt atgtgacgtc aaaattttat aagggtgaag
     3421 tattatgaag actgttagga tacccgatga tgctttaaga caatactata atgattcggc
     3481 agagttttct aagataattg aatattttaa aaaatccggg ataaagcatg aagtaaatat
     3541 agacgccctg acagggtata agtttattca atacggtgat aacgatcgaa gtatgtatat
     3601 agttaaggct gatgaaggag tctatctgcc ggagagtaga tataaatttt ttcctaagga
     3661 attacttagc gaaaaaaggg tcataacaat tggttttgag aataaccata ttaaacccgg
     3721 acagagtgta actctataca ttgatactcc acgttcaata tataatgtaa aagtgttttt
     3781 ggaaaatatt aaaacaaaag aaaggaaaca gctatataat ataattccgg aaatgcttta
     3841 taggggaaag attaatgttt attggattgc tgataatgac ataaaactgg gggaatatag
     3901 ggcagtatta atatggtctg agtatgaaag caggcgaaaa gaatctgttt catccgtgtc
     3961 tgtattaaag gttataacag atacaggcat aagactggga aaatattttt tatcaaacgg
     4021 atcagatcat atttatatat atactagaaa ggttttgaaa gaggagctgt accatgatga
     4081 aaaaagtaag gaggaagcat tcaaaaagct agtcctgttt ataatggaga acagcaagag
     4141 ggaggaatcc tatgcagtaa ttgagactgc agcttccggc tcaaacaaga ggaacatgct
     4201 caggctaatg acaattaatc acaattttag tattaaggat ttacataaag acggcaatgt
     4261 aaaaaatttg ctgcctccat cgccgcagga gttggctgaa ttggaatcgg aaaatagaaa
     4321 aatagaaagt ataataatcg gcaattatgt acagttatgt cttggtttgg atgaaatcgg
     4381 gaaatatgtt tacaaatgtg cagatattat ttctgaaaac tataacttgg acgatgaggt
     4441 tttttttaag tttaaatttt ctaataaaat aaaagagtat tctttttcta tcgaatcaat
     4501 taaaggggca catacaaacc gatatattct tccgtttgag ttttcgcaat atatacaaat
     4561 taaattgctg c
//
LOCUS       DAOOJC010000171         4569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14859_length_4569_cov_3.245680, whole genome shotgun sequence.
ACCESSION   DAOOJC010000171 DAOOJC010000000
VERSION     DAOOJC010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14859_length_4569_cov_3.245680"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..698)
                     /locus_tag="PLA01_05735"
     CDS             complement(273..698)
                     /locus_tag="PLA01_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00834.1"
                     /translation="MIYPRSGLRIRSSKGVHSEVRRACIEFGKWLRKTNNFPIRVVIY
                     LKKEYRIRNMEGELVTATFFAPFEKNVEPYIRIATGDYEELYEKKGKDSALVAYLYSI
                     AHEIVHYKQWIKDSDFDEKRAIVESKRMIDAYSGTREHP"
     gene            complement(724..1182)
                     /locus_tag="PLA01_05740"
     CDS             complement(724..1182)
                     /locus_tag="PLA01_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00835.1"
                     /translation="MTRNLGENPAELLIGNIFAYCGNNPVIYTDVSGMWFGIDDAFTG
                     PVDEIIVLGVLAILATCGVEWASDAQDEIWDVITDAWDSVKSLSSYIKDWISEARQGP
                     VGKAGRKKQGREVNEKKRRDDDWIPRSNKDPNRPMKKHTPGRDYRIRKKR"
     gene            complement(1237..1740)
                     /locus_tag="PLA01_05745"
     CDS             complement(1237..1740)
                     /locus_tag="PLA01_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HPD00836.1"
                     /translation="MDKTIIYNYDVGGNITSKTEYAYTKNQNPGTPIRTINYTYDTGK
                     RYEKTANAVTTRYHLVGDRVTYETNGTDSIYYTYDANGSLLSMNLNGNEYYYIRNLQG
                     DITGIIDSTGEQVVSYTYDTWGKLISMTGPLASTVGEKNPYRYRGYRYDRETGLYYLQ
                     SRYYNPE"
     gene            1877..2896
                     /locus_tag="PLA01_05750"
     CDS             1877..2896
                     /locus_tag="PLA01_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2600 family protein"
                     /protein_id="HPD00837.1"
                     /translation="MNNRCIYGLRLMIKYINSKYPKLYKIVLDYKLFHQEEIDSEKCI
                     GAAFGILYPGANPENTINFIASFHAIDLYVYSLCASFHKKDVFLFSTIYNSLRDAVDP
                     KRKISVEQIDCLQKGDVQNITRLIEQCRIHINTLPSYSVVIGQLKKFIQMYSDFRIYS
                     LFDKDTREKKIETWAQRYHDENVGISKWEMSAASNSTLLILVLFACAHDPHLTGEEVD
                     NICSSYFPWICGLQVLLRDFVNASEDLQQGKPNLTYYYKNLKTCEERISVFIERALES
                     CNNLKYPHFHRTIVEGVIAIYLSNPKAFYGMNRLASMSLIKKGGSNANTYYNLCRFLR
                     SSGKL"
     gene            complement(2920..3450)
                     /locus_tag="PLA01_05755"
     CDS             complement(2920..3450)
                     /locus_tag="PLA01_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HPD00838.1"
                     /translation="MNIVKELSIYAIPAIFIIILCVGMYKEVKVYDVFIEGAKEGITT
                     VFKIIPSLVGLMVAVAVFRASGALNLLIFAVKPVTNFLRIPPDALPMVFLRPISGSAA
                     LAMLSDIFKVHGPDSFVGRVASTLMGSTETIFYTLAVYFGAVGVKKIRYTLAAALIAD
                     LAGVIAAVWICTLVFG"
     gene            complement(3447..4067)
                     /locus_tag="PLA01_05760"
     CDS             complement(3447..4067)
                     /locus_tag="PLA01_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPD00839.1"
                     /translation="MLNYIWFVMLVLGIAVGIMNGRVDEVTQAVVNSSQNAIELGIGL
                     LGIMCLWTGIMNIAERSGIIKKISKAIRPVMSFLFPGIPKEHPAGGAIVMNLVANFLG
                     LGNAATPLGLKAMNELQKLNPDKDVATDEMSMFLVLNTACLQFIPATVIALRAAAGSK
                     NPTEVIGTIWVATLCAATAGVVAAKIFSSLLGNKPRHAKRTGRGKS"
BASE COUNT         1383 a         1006 c          745 g         1435 t
ORIGIN      
        1 tatataaagc attataaacg taaatgctcc aatttattat tgccttccat gtttgccatt
       61 aatactaata tacatatata ttggagattt ttcaaggaaa ttcacaccac aggcatcaat
      121 aagccatttt tcctctttta attttaagat ctctttactc tttgtttcaa gactacataa
      181 aaagtccact tttacagcgt tttctacatg tacttttatc cctgataaga tattaattca
      241 agtaactaca ttaaatacta tccttcattt tctcaaggat gttctcttgt tcctgaatat
      301 gcatcaatca ttctcttact ttcaacaatt gctcttttct catcaaaatc ggaatcttta
      361 atccattgtt tataatgtac aatttcatgt gctatagaat ataagtaagc tactaatgca
      421 ctatccttac ctttcttttc atataattct tcatagtccc cagtggcaat ccttatataa
      481 ggctcaacat ttttttcaaa tggagcaaaa aatgttgctg taactaattc accctccata
      541 tttctaattc gatactcttt tttaaggtat ataacgactc taatcggaaa gttatttgtt
      601 ttccttaacc acttaccaaa ctcaatgcat gcccttctca cttctgaatg tacacccttt
      661 gaacttctta ttcttaaacc acttctagga taaatcatgt tgatcgtcct ttcacaaata
      721 actttaacgt ttttttctta tacggtaatc acgccctgga gtatgttttt tcattggacg
      781 attaggatct ttattagaac gcggaatcca gtcatcatcc cttctctttt tttcattcac
      841 ttctcgacct tgtttttttc ttcctgcttt tccaacaggg ccttgtctag cttctgatat
      901 ccaatctttt atatagcttg ataaactctt aactgaatcc catgcatctg tgattacgtc
      961 ccagatttca tcttgtgcat cagaagccca ctcaacccca catgtagcta atattgctaa
     1021 aacacctaaa acaataattt catcaacagg acctgtaaag gcgtcatcaa tgccaaacca
     1081 cattccactt acatctgtat atataaccgg attattacca caataggcaa atatatttcc
     1141 tatcagcagc tctgcaggat tctcacctag gtttcttgtc acttcttctg ctagtgtctt
     1201 gagtacttcc gctgcatccg cattaataaa cctgccctat tctgggttat aatacctact
     1261 ctgcaaataa tacaaacctg tctctctgtc gtacctgtaa cctctatacc tgtaaggatt
     1321 cttttcccct accgtacttg ccaatggacc cgtcatgctt attaacttac cccatgtatc
     1381 ataagtataa cttaccacct gctctcctgt gctgtcaata attcctgtaa tgtctccctg
     1441 taaattccgg atatagtagt actcatttcc attcaggttc atactcaaaa ggcttccgtt
     1501 tgcatcatag gtataataaa tactgtctgt accgtttgtt tcataggtta ctctgtcacc
     1561 gactaaatga taccttgtag taacagcgtt tgctgtcttt tcatatcttt tgccggtatc
     1621 atatgtatag tttatagttc ttatcggagt ccctggattt tggtttttcg tataagcata
     1681 ctctgtcttg cttgttatgt ttccacctac atcgtaattg tatatgattg ttttgtccaa
     1741 tacctgatat ttgacaacga ttatcctgaa atacacaaaa aaattagaat aagacagtaa
     1801 acgaatttgt aaaagcatag tatatcacag actgtttatg cataaaatgg gaagaataca
     1861 ataaaggagg gattttatga acaaccggtg catatacggc ctgagattaa tgataaagta
     1921 cataaacagc aagtatccga agttgtataa gatagttttg gactataaat tatttcatca
     1981 agaagagatt gacagtgaaa aatgcatagg agcggccttt ggcatacttt atcccggtgc
     2041 gaaccctgaa aacaccataa acttcatagc ctccttccac gccattgatc tatacgtata
     2101 ttctctctgt gccagtttcc acaaaaagga cgtatttctt ttcagcacga tttataattc
     2161 cttgagagat gcggtggatc ccaaaagaaa aatctcggtg gaacaaattg attgcttgca
     2221 aaagggagac gtccaaaaca tcacccggct tatagaacag tgcaggatac atattaatac
     2281 gctgccctcc tatagtgttg tcataggcca gctgaaaaag tttatccaga tgtactcgga
     2341 ctttcggata tattctcttt tcgacaaaga tacaagagaa aagaaaattg aaacctgggc
     2401 tcaaaggtat catgacgaaa acgtagggat atccaaatgg gagatgagtg ccgcttcgaa
     2461 ttccacactg cttatattgg ttctctttgc ctgtgcccat gaccctcacc ttacagggga
     2521 agaggtcgat aacatatgct cttcctattt cccatggatt tgcggccttc aggttctgct
     2581 ccgcgacttt gtgaacgcca gcgaagacct tcaacaggga aaacccaatc tcacatacta
     2641 ctacaagaac ttaaaaacgt gcgaggaaag aatttccgta tttatcgaac gggccttgga
     2701 aagctgtaac aatctgaaat accctcattt tcaccgaacg attgttgaag gagtcatcgc
     2761 catatacttg tcaaacccca aagccttcta cggaatgaac aggttggcca gtatgagtct
     2821 gataaaaaag gggggttcaa acgcaaatac gtattacaat ctgtgcagat ttttaaggtc
     2881 ttccggaaag ctttaattca tcattcagaa aaataattat cagccaaaaa ccagggtgca
     2941 tatccacact gccgcaatta cacccgccag atctgctata agggccgctg ccagggtata
     3001 cctgatcttt tttacaccca cggctccaaa gtatactgcc agagtataaa atatggtttc
     3061 ggtagaaccc atcaaggttg atgccacgcg gccgacaaaa gaatccgggc cgtgcacctt
     3121 gaatatgtcg gacagcatcg ccagggctgc actccctgaa atagggcgga ggaaaaccat
     3181 gggaagtgca tccggaggaa tccttaaaaa attagtaacg ggtttaaccg caaaaatcag
     3241 aagattcaat gcacccgacg ccctgaacac tgccaccgca accataagtc ccaccagcga
     3301 aggaataatc ttaaatacag tagttatccc ctctttggca ccctctataa aaacatcata
     3361 aaccttcact tctttataca tacccacaca taatatgatt ataaaaattg caggtatggc
     3421 atatatcgaa agttctttta ctatattcat gacttccccc gtccggtcct ttttgcatgc
     3481 cttggtttat tgccgagtaa agacgaaaaa attttggcag caaccacccc tgcagtagca
     3541 gcacacaggg tagccaccca tatggtaccg attacttccg tagggttctt tgagcctgcg
     3601 gcggctctca gtgcaataac cgttgcaggt ataaactgca aacaggcagt atttaacacc
     3661 agaaacatgc tcatctcatc ggtagccaca tccttgtcgg gattaagctt ctgtaactcg
     3721 ttcatggcct tcagccccaa gggggttgcc gcattgccaa gccccaaaaa attggcaaca
     3781 aggttcatta caatagctcc accggcagga tgttccttcg gtatgcctgg aaacaaaaaa
     3841 ctcatgaccg gccttatagc tttcgaaatc ttcttaataa tgccacttct ctctgcaatg
     3901 ttcatgatcc cggtccaaag acacataatt ccaagaaggc ctatcccaag ctcaatcgca
     3961 ttctgagagg aattcaccac cgcctgggtt acttcatcca cccttccgtt cattatcccc
     4021 acagcaatgc ccaagaccag cattacaaac caaatgtaat ttaacacagc aaacctcaca
     4081 cttttaggga gcacattatt atatagaaga aaaatatata agaagcttta gtttctgaaa
     4141 ttttttcttc ttactttatt tgacgcaccc tgcttttaat tgtacatacc taataatgta
     4201 tatgaggata cttattataa tatgaaaaat caaatttttg aataaatggt tttaatgtgg
     4261 gtatatatta tgtaagcaaa gaaaaaagga ggtaaattaa aaattttggc gaatttcagt
     4321 atatgtgccg ggtatagagt ttaccagtaa tatattggat ggatagccct tttatccttg
     4381 tatttttaaa aaacactagt ttattgacca attgacttgt attttagtta gagtctatca
     4441 aatgcaagtt aatagtaact taataaaaaa ccgtgttata ttattgtatt aattccataa
     4501 tttattattg tattaaattg aattttttaa aaataattgt tgactattat tggcatttat
     4561 ggtatcatg
//
LOCUS       DAOOJC010000172         4565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14871_length_4565_cov_3.585809, whole genome shotgun sequence.
ACCESSION   DAOOJC010000172 DAOOJC010000000
VERSION     DAOOJC010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14871_length_4565_cov_3.585809"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..703
                     /locus_tag="PLA01_05765"
     CDS             344..703
                     /locus_tag="PLA01_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HPD00840.1"
                     /translation="MEKYKLFDAEYKFVSIIWDNEPINSTELVKLCADKLGWKKSTTY
                     TVLKKLCERGVLKNKDATVTSLVKREDVQKYESRAVLEKNFDGSLPKFLTAFLSGRKL
                     SEKEAEELKRIIEEAVK"
     gene            956..1213
                     /locus_tag="PLA01_05770"
     CDS             956..1213
                     /locus_tag="PLA01_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00841.1"
                     /translation="MVKSGINSAVTASLPASVPSTSMNVIQICITVFSFVWIFGVVVL
                     IFYSIFSCLKIRRKLRTATIVKDNVFETDAVKRDGTRKQTL"
     gene            1219..1410
                     /locus_tag="PLA01_05775"
     CDS             1219..1410
                     /locus_tag="PLA01_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00842.1"
                     /translation="MQIDFIRITFEDGTVVEEEVSMDKGYIVIADTEAGIKNIEVFNK
                     QGELQSDLSETIYWEQTWF"
     gene            1570..>4565
                     /locus_tag="PLA01_05780"
     CDS             1570..>4565
                     /locus_tag="PLA01_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPD00843.1"
                     /translation="MRLKRAISLLLIVCIIFAFNPSLFASRGAYDRNGFRLVPVDSDS
                     TGIAVDSEFLLETEKDFTLEEVKDAFSIDGEPDPIIEELDPNFFSIRLSRPLLENTVY
                     TFRMSTDIETTWVFQTQTEFKILGSLPGNESVGVPVNTGIEIYFSHSDFSNLEDYFEI
                     TPYVRGRFEVHDKTAVFVPNGLTEETVYTVRIKKGIKIEGTERAIKDDYVFSFETKTK
                     STSDTTEPTIYINYNRQITDFLPEEKPQLPFSYYIMNAGASYKEVILYTGVYAFKDFE
                     SFYEAVCVKNNIPQWAQASYSKNYVPVDGLEKVLEFKQILNEGGRMSGNYKIEIPQNL
                     PEGYYIVESLCEGKRAQTFIQVTNIGTYITKSDTKTLVWLNDLNSGEPLGGAAISFSN
                     VNDSFYTDKKGIATFDTELINYVSNETYSWKTQLMSVKTADGRKSLLDTTSYVYNYDY
                     YYGDYYYYNYNSSNNKYWRYFFTDRDMYKPDDTVNVWGFIKNRYEDEEIDYLTLEIYQ
                     SWYYGSGRIPIIKENISVKDNFFEGQVELPNLPKNYYTLLLKKGNTTISNSVIRVEDY
                     VKPSYKMQIEKDKQAVFIGDKVNFKLNAAFFEGTGVSNLNVNYTIGTAALTGSSITQN
                     TVTDTTGNLDIEYIPEVTKDGLQGITSVGINARALLPESGEITAYNWVRLFINDIDVS
                     LKGEINNGKGTINAKVHEIVLDRLNNGTAQNNNDYLGDPVNSKRIVGTIYKNTWLKEE
                     SGTYYDYINKVTRKTYRYWVETTPIDSISMTTSSNGEASYTFNAPKIEDTYYTAVLSC
                     IDNSGRSMKFEIYIGEKINYSGSKNTIFALNSDKGSYRIGEDVKLDFTFSNENLPDGD
                     YIYILSQNGIRSYDTSDKSSYTFEFKDEYMPNVQATAVYFSGKGYVSRSMNIKYDMSE
                     KNLVIKAETDKSSYKPGERVTLKVYVTDIYGNPVKACVNASIVDEAFFSLDEQYVNVL
                     ERLFENVSSGVYFEAVSHDIKENIYRG"
BASE COUNT         1511 a          727 c          975 g         1352 t
ORIGIN      
        1 aagcaaaagg ataaagacca tacagacagt aggaagtatg agggacctta aattgattac
       61 atttatttgt ttcaatgaaa tagccagcat taccggtact atgattataa ttgctacaaa
      121 gtatggagtc atatgctatc cccgtaattt ataacttgcc tcttggttta ttctatttgg
      181 ttgttatgaa aaatagaact tggtttaata cataaaaata cagtttaatt tgttgatggt
      241 attgaatttt ttgcgattaa tttgagtttc gctattgaca aatcaggtct aaaacgatag
      301 actttaaccg ggtctattaa aataaaccta gggaggcaaa tatatggaaa aatataagct
      361 gtttgatgcg gaatacaagt ttgtcagcat tatctgggac aacgaaccta tcaattccac
      421 tgaacttgta aagctgtgtg cagataaact tgggtggaaa aaatcaacta cttatactgt
      481 attaaaaaag ctctgtgaac gcggtgtttt gaaaaacaag gatgcgacag tcacttcact
      541 ggtaaagcgt gaagatgtgc aaaaatatga aagcagggct gtattggaaa aaaactttga
      601 cggttcactt ccgaaatttc tgaccgcctt tttgagcggc cgcaagcttt ctgaaaagga
      661 agccgaggag cttaagcgga ttatagagga ggcggttaaa tgagtgtact tcaaaattta
      721 tttatatcta tttttaaaat gagtattact gcatcctatg tggctattgg tgttattttt
      781 atacgtatgt ttctaaaaaa atgccgaaga ttttttccta tattctttgg gttctggtgt
      841 ttttccgcct tttatgcccc ttttctttta attcggcttt gagtttttta aacttggtaa
      901 accttaattc aaagcatgga agtgggtatc tgagtttata ccgcaaaatc cgggcatggt
      961 taagtccggt attaacagtg ctgtaactgc ttctttgccc gcatccgttc cgtcaacaag
     1021 catgaatgtt atacagatat gtataacggt tttcagtttt gtttggattt ttggtgttgt
     1081 tgtgctgata ttttacagca ttttttcctg cctgaaaatc agaagaaagc ttcggactgc
     1141 gacaattgta aaagacaatg tctttgaaac cgatgccgta aaaagagatg ggacccgaaa
     1201 acagaccttg taaacgatgt gcaaattgac tttatcagga ttacttttga agacggaacc
     1261 gtagttgagg aagaggtttc catggataag ggatatatag ttattgccga taccgaggca
     1321 ggtataaaaa atattgaagt ttttaacaag cagggagaac tccaaagcga tttgagtgag
     1381 accatttatt gggaacaaac ttggttttaa tagatggaaa aaaagtccta tcataaaaaa
     1441 ggacgaaaag gattcagatt gaagtaaaaa aatatgtaca taaagccgga aaaaggaaaa
     1501 atattgtttc caattcacca tattgtatta tataattata atgtaagaaa ataatcgggg
     1561 gggagtagaa tgaggcttaa aagagcaatt tctttattat tgattgtgtg cataattttt
     1621 gcattcaatc cgtcgctttt tgcatcacgg ggggcttatg acaggaacgg ctttcgtctt
     1681 gtaccggttg acagtgattc gaccggcatt gcggttgaca gcgaattttt attggagaca
     1741 gaaaaggatt ttactttaga ggaggtaaaa gacgcttttt caattgacgg tgagcctgac
     1801 ccaattatcg aagagcttga cccgaacttt ttcagcatta ggctttcaag accgcttttg
     1861 gagaacaccg tttatacctt cagaatgagt actgatattg agacaacatg ggtttttcaa
     1921 acgcagacag agtttaaaat attgggttct ctgcccggaa acgaatcggt gggagtcccg
     1981 gtaaacacag gaatagaaat atatttcagc cactcggatt tcagcaattt agaggattat
     2041 tttgaaatta ctccttatgt tcgcggaagg tttgaggtac acgataaaac agctgtgttt
     2101 gtaccaaatg ggctgacaga agagacggtc tacactgtta gaattaaaaa aggaattaag
     2161 atcgaaggta ccgaaagagc aataaaagat gattatgtat tttcctttga aacaaaaaca
     2221 aaaagcactt ctgatactac tgagcccaca atatatatta attataaccg gcagataaca
     2281 gatttcttac ctgaggaaaa acctcagtta cctttcagct attacataat gaatgcagga
     2341 gcaagttata aagaagtaat cctttatact ggggtttatg cttttaagga tttcgagtct
     2401 ttttatgaag cagtgtgcgt taaaaacaat attcctcaat gggcacaagc tagctattcc
     2461 aagaattacg ttcctgttga cgggcttgaa aaagttttgg aatttaagca gattttgaac
     2521 gaaggcggac ggatgtcggg aaactataag attgagattc ctcaaaacct tccggaggga
     2581 tattatattg tggaaagcct ctgtgagggt aaaagagctc agacctttat tcaggttacc
     2641 aatataggta catatataac aaagagtgac accaaaaccc tggtatggtt aaatgacttg
     2701 aattcgggag aacctttggg tggtgctgct attagctttt ccaatgttaa tgactccttc
     2761 tatacagata aaaaaggaat agcaaccttt gacactgagc taattaatta tgtcagcaat
     2821 gagacttaca gctggaaaac ccagcttatg tctgtgaaga ctgctgatgg cagaaagtct
     2881 cttttagata ctacatcgta cgtttataac tatgattact attatggaga ctactattac
     2941 tataattata atagtagcaa taacaaatat tggagatatt tctttaccga cagggatatg
     3001 tacaaacccg atgataccgt taatgtttgg ggatttatta aaaacaggta tgaggatgaa
     3061 gaaattgatt atctgacgtt ggagatttat cagtcgtggt actacgggtc cggtcgtatt
     3121 cccattatta aagaaaacat aagtgtgaag gacaacttct ttgaggggca ggtagaactt
     3181 ccgaatttac cgaaaaatta ttacacccta ttgctcaaga aaggcaatac cactatttcc
     3241 aacagtgtta tccgggtaga ggattatgtg aagccttcat acaaaatgca gattgaaaag
     3301 gataagcagg cagtgtttat cggggataag gttaatttca aactcaatgc ggcatttttc
     3361 gaaggaaccg gagtatcaaa tttgaatgtg aattatacca ttggtacagc tgctcttacc
     3421 ggaagttcca ttacccaaaa tactgttacg gataccactg ggaatctgga tatagagtat
     3481 atacctgagg ttaccaaaga cggtttgcag ggaataacct cagtcggcat taatgccaga
     3541 gcccttcttc cggaatcggg agaaataact gcttataatt gggtgaggct atttataaac
     3601 gatatagatg taagccttaa gggagaaata aataatggaa aaggaactat aaacgccaag
     3661 gttcatgaaa ttgtccttga ccgccttaac aacggaacgg cacagaataa taacgattat
     3721 ttgggagatc ccgtaaattc caagaggatc gtcgggacaa tatataagaa tacctggctt
     3781 aaagaagagt cgggtactta ctatgactat ataaacaagg ttacccgcaa aacgtacaga
     3841 tattgggtcg agacaacacc gattgacagc atttcgatga caacatcctc caacggagaa
     3901 gcttcttata cctttaatgc accgaagatt gaagatactt actatactgc agtattatca
     3961 tgtattgaca attcgggaag gagtatgaag tttgaaatat atataggaga aaagattaac
     4021 tatagcggtt ctaaaaacac catatttgct cttaatagtg ataaaggcag ctacagaata
     4081 ggtgaagatg tcaaacttga ttttaccttc tcaaatgaga atcttcccga cggtgactat
     4141 atttacatac tgtcgcaaaa cggaatcaga agctatgata catcggacaa gtcaagctat
     4201 acctttgagt ttaaagacga atacatgccc aatgttcaag ctacggcagt atattttagc
     4261 ggtaaaggct atgtgtcaag aagtatgaat attaaatatg acatgagcga aaaaaatctt
     4321 gttattaaag ccgaaaccga taaaagttct tacaagccgg gcgaaagggt tactttaaaa
     4381 gtttatgtca cggatattta cggaaatcct gtgaaggctt gcgtaaatgc aagtatagtt
     4441 gatgaagcct ttttcagtct ggatgagcag tacgttaatg tgcttgaaag attgtttgaa
     4501 aatgtttctt cgggagtata ttttgaagcg gtgtcccatg atataaaaga gaatatttac
     4561 cgagg
//
LOCUS       DAOOJC010000173         4563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14883_length_4563_cov_2.979592, whole genome shotgun sequence.
ACCESSION   DAOOJC010000173 DAOOJC010000000
VERSION     DAOOJC010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14883_length_4563_cov_2.979592"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1003
                     /locus_tag="PLA01_05785"
     CDS             26..1003
                     /locus_tag="PLA01_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPD00844.1"
                     /translation="MESLIEVSLEFDRIEHAMNLFGNFDENINIVEDAFNVKIISRDN
                     EVRVVGCSDAVYKAQTVLQRLISLAAQGDTITKQNVSYLVQLADEDQLDRVKDFRADC
                     ICVTARGKQIKSKTHGQKMYVDAIKENDIVFGIGPAGTGKTFLAVAMAVTAFRNKEIN
                     RIVLTRPAVEAGEKLGFLPGDLQNKVDPYLRPLYDALYEMMGFETYHKYLEKGMIEVA
                     PLAYMRGRTLDDSFIILDEAQNTTPEQMKMFLTRIGFGSKAVINGDVTQVDLPGDKKS
                     GLKEVINVLKDIKGVSFVYLSDKDVVRHELVQRIIQAYERYDKVAKNHF"
     gene            975..3260
                     /locus_tag="PLA01_05790"
     CDS             975..3260
                     /locus_tag="PLA01_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPD00845.1"
                     /translation="MIRLRKIIFKDNGLKGDKNKEKNRESKPDNSFWRGYFKDKHIQR
                     VFIVTVTVILAFLIVLNGATPKRYRLTLGEKSNYDIHAPRDIVNTVKTEENARKAAAE
                     VTPDMKEIRNASIEVINSVVSLKSLIEKAQKDYKASIASIGTSSGDVYKELAQKELEK
                     ASAALQESINKLGITLRNAQTEYLISETSIDDISNFEGVLRGLVSDVMKKDITDDNLK
                     DRITELKNGVEDSDLRQELKDVAIAVSNYVLKPNRTINEELTEAKRNAAYNDPKNVEI
                     IKKGQRILSVDDTVTEDKLKVLEDLNLLETKSKFDFALAGGILVVLVLLFLLLILYMN
                     NFCKKVFYNRSHLILMSIVIILTLLAAMAVHEYSTLIIPIFIATMLISILLDLRLALV
                     VNIVLTIAISLMIGSDDKFVYMALITGSFSAFIVSKANQRNKLSFAGTVVAALNVLVI
                     TAINIINKSAWDVSLREGIIVFINGIASMLVTNWILPFMESTFNIITPLRLLELANPN
                     QPLLKKLLMEAPGTYHHSLMVGNLAEAGTEAIGGNALLARVGAYFHDIGKLKRPSFFI
                     ENQMGDNPHDKMTPNLSALVIISHARDGYEMAKKYKIPLAIRDIILQHHGTTLVAYFY
                     HKAKKCEKMDNVAEANFRYDGVKPASREAAVVMLADSVEAAVRSMTDKTEGKIEGLIR
                     KIIKDKLDDGQLDHCSLTLKDLDTIAKAFVKTFGGFFHEREEYPALKDTANRLKSNDS
                     EDYNEAVEKTVSEKQEIKGEK"
     gene            3261..3746
                     /gene="ybeY"
                     /locus_tag="PLA01_05795"
     CDS             3261..3746
                     /gene="ybeY"
                     /locus_tag="PLA01_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPD00846.1"
                     /translation="MEIFIENLQDKIEVTDEILKNMDKAARICLENENFTLPYEISVI
                     LIDNDRIKEINNEQRGIDKPTDVLSFPIVDMYEGEIKSSDGDFDMDEGRIVLGDILIS
                     LEKVKEQSIEYGHSFERELIFLLTHGVFHLLGYDHDTEERERRMLERQNAVLKSLNLE
                     R"
     gene            3755..4456
                     /locus_tag="PLA01_05800"
     CDS             3755..4456
                     /locus_tag="PLA01_05800"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HPD00847.1"
                     /translation="MKSRNIIDSFNYAIKGIIHAVKSERNMKIHIAFGIAVFLLSLFF
                     DLTKTEFMIICLTVAMVLISEMFNTAIEVVVDLIEEKYNLKAKIIKDVSAGAVLVSAF
                     FAVIIGYFIFFERASTGLETGIERIKQYPMHVIVIILAITVLVVVVLKAFSRKGTPLR
                     GGMPSGHAAIASALSTAVALWSVDIKITVLCVVLSSLVIQSRLEAKIHSFLEVFFGAV
                     IGFVITLLLFRIFLT"
BASE COUNT         1419 a          733 c         1116 g         1295 t
ORIGIN      
        1 aaacatacag attggagggg aatatttgga aagtcttata gaggtatccc tggaatttga
       61 cagaatcgag catgcgatga acctctttgg caattttgac gagaatatca atatagttga
      121 ggatgctttc aatgtaaaga ttatttccag ggataatgag gtcagggttg tcggttgcag
      181 tgatgcagta tataaagcac aaacggttct tcagcggctt attagtttgg cagcccaggg
      241 agatacgata acaaaacaga atgtaagtta tcttgttcag ttggctgatg aggaccaatt
      301 agacagggtt aaggattttc gggccgactg tatttgtgtg acagcaaggg gaaagcagat
      361 taagtccaag acccatgggc agaaaatgta cgttgatgca ataaaggaga acgatatagt
      421 attcggaatc ggtccggcag gtaccggaaa gacgttttta gctgttgcca tggcggtgac
      481 ggctttcaga aacaaggaga ttaacaggat tgttcttacc cggcctgctg ttgaggcggg
      541 agagaagctg gggtttttac ccggtgactt gcagaacaaa gtagatcctt atttaaggcc
      601 gttatacgat gcattgtatg agatgatggg atttgaaacc taccacaagt atcttgaaaa
      661 gggaatgatt gaagtggcac cccttgccta tatgagggga aggacactgg atgattcttt
      721 tattatactt gatgaagccc agaatactac cccggagcaa atgaagatgt ttttgacacg
      781 aataggtttc ggttcaaagg cggtaattaa cggtgatgtg actcaggtag accttcccgg
      841 ggataaaaaa tccgggttaa aagaggttat taacgtatta aaggatataa agggagtttc
      901 atttgtttat ctgtctgata aggatgttgt aaggcacgaa ctggttcaaa gaatcataca
      961 ggcatatgaa aggtatgata aggttgcgaa aaatcatttt taaggacaat ggcttgaaag
     1021 gtgacaaaaa caaggaaaag aacagggaaa gcaaaccgga taacagcttc tggaggggat
     1081 attttaagga caagcacatc cagagggtgt ttattgttac cgtgacggtt atccttgcct
     1141 ttcttattgt tttaaacggt gcaactccaa agagataccg gcttacattg ggggaaaagt
     1201 ccaattacga cattcatgca cccagggaca tagtaaatac cgtcaagacg gaggaaaatg
     1261 ctagaaaagc agcagcggaa gtgactcccg atatgaagga gataagaaat gcatcgatag
     1321 aggttataaa cagcgttgtc agcttgaagt ccttgattga aaaggctcaa aaggattata
     1381 aggcttctat cgcttctatt gggacaagtt ccggtgatgt atataaggag cttgcccaaa
     1441 aagagcttga aaaagcttca gcagctcttc aagagagtat aaacaagctt ggaattaccc
     1501 ttagaaatgc tcagactgag taccttatat ctgagacaag cattgatgac atcagtaatt
     1561 ttgaaggtgt tctcagaggg ctggtaagcg atgttatgaa gaaggatatt actgatgaca
     1621 accttaagga tagaattaca gagcttaaaa atggtgttga ggacagcgat ttgaggcagg
     1681 agctcaagga tgttgccata gctgtctcaa attacgtctt gaagcctaac agaactatta
     1741 atgaggagct tacggaagcc aagagaaacg ctgcttacaa tgaccctaaa aacgtagaga
     1801 ttattaagaa aggacaaagg atattaagtg tcgacgatac agtcactgag gacaagctta
     1861 aggtattgga agatctgaat ctgcttgaga caaaaagcaa atttgatttt gcccttgccg
     1921 gaggaatact tgtggtattg gtgctgttat tcctccttct tatactgtat atgaacaatt
     1981 tttgcaagaa ggtgttttat aacagaagtc acctgatact tatgtctatt gtcattatac
     2041 tgacgctttt ggctgcgatg gcagtacatg agtattctac tttgataata ccgatattta
     2101 tagccacaat gcttatttca attttacttg atttaaggct tgcccttgtg gtaaatatag
     2161 tattgaccat agcaatatca ttgatgattg gcagtgatga caaatttgtg tatatggctt
     2221 tgataaccgg ctccttttct gcttttatcg tttcaaaggc caaccaaaga aataagctgt
     2281 cctttgcagg aaccgttgtt gcagcactta atgtgctcgt tatcacagcc attaatatta
     2341 tcaacaagtc tgcatgggat gtatcgttaa gagaaggtat tattgttttt ataaacggta
     2401 ttgcatcaat gcttgttacc aattggatac ttccgtttat ggaaagtacc tttaacataa
     2461 tcacaccctt aaggcttttg gagcttgcca atccgaacca gccgcttctt aaaaagctgc
     2521 ttatggaagc cccgggaact tatcatcaca gtctgatggt ggggaacctt gcagaggcgg
     2581 ggacggaagc tattggcggg aatgcattac tggcaagggt tggggcatat tttcacgata
     2641 tagggaagct caaaagacct agctttttta tcgaaaacca gatgggggat aatcctcatg
     2701 acaagatgac tccaaacctc agtgcccttg taattatttc ccatgcccgt gatgggtatg
     2761 aaatggccaa aaagtataaa attcctctgg cgattcggga tataatatta cagcaccatg
     2821 gaaccacctt ggtggcttat ttctaccata aagcaaaaaa atgtgaaaag atggataatg
     2881 tcgctgaagc aaatttcagg tatgacggtg tcaagccggc atcgagggaa gctgcggtgg
     2941 taatgctggc cgattctgtg gaggcggcgg taaggtctat gaccgacaaa accgaaggaa
     3001 aaattgaagg gttgataaga aagataataa aggataaatt ggatgacggt cagttggatc
     3061 attgcagttt aactcttaag gatcttgata ccattgcaaa ggcttttgta aaaacctttg
     3121 gaggtttctt ccatgaaaga gaagagtatc cggctttgaa ggatacggca aacaggctta
     3181 aatccaatga ttctgaagat tataacgagg ctgttgaaaa aacggtgtca gaaaaacagg
     3241 agataaaggg agaaaaatag atggaaatat ttattgaaaa tttacaggat aaaatagaag
     3301 taacggatga aatcttaaag aatatggaca aggccgcaag gatttgcctt gaaaatgaga
     3361 actttacttt accttatgaa ataagtgtga ttcttattga caatgacagg attaaagaaa
     3421 tcaataacga gcaaagagga attgacaaac ctactgatgt tttatccttt cctattgtag
     3481 atatgtacga aggggagata aaatccagtg acggtgattt tgacatggac gaagggcgta
     3541 ttgtgttggg ggacatacta atctcgctgg aaaaggtgaa ggagcaatcc attgagtacg
     3601 gacattcctt tgaaagggag ctgattttcc tcttgaccca tggcgttttt cacctgctgg
     3661 gttatgatca tgacactgag gaaagagaaa gaaggatgct ggaaagacag aatgccgttt
     3721 taaagagtct gaaccttgaa aggtaatggg gcttatgaaa agcaggaata tcattgacag
     3781 cttcaactat gcaattaaag gcataattca cgcagttaaa agcgagcgta atatgaaaat
     3841 ccatattgca tttggaatag ccgtgttctt gttaagtctg ttttttgatc ttaccaagac
     3901 ggagtttatg attatttgtc ttacggtggc aatggtttta atatctgaaa tgtttaatac
     3961 ggctattgaa gtggtagtag atctgataga agaaaagtac aatctcaagg ctaaaattat
     4021 caaggatgtt tctgccggtg ccgttttggt ttctgctttt tttgctgtca ttataggata
     4081 ttttatattt ttcgaaagag caagtaccgg gttggaaacg ggtattgaac ggattaagca
     4141 gtatcccatg catgttattg ttattatact tgccattacc gtattggtag tggtggtttt
     4201 aaaggcgttt tcccgaaagg gtacgccgct gaggggcgga atgcccagcg ggcatgcggc
     4261 aattgcatcg gctttatcca cggcggttgc tttgtggtcc gttgatatta aaattacagt
     4321 gttgtgtgtg gttttatcga gccttgtgat tcaaagcagg cttgaagcca aaatacactc
     4381 gtttttggaa gtgttctttg gtgcagtgat tggttttgta attacattac tgctgtttcg
     4441 aatttttctg acataaatta cggcttgtac cggaggtgga agaaatggac gataaagaat
     4501 tattgcttct tgcagaagaa ataaaaaaga actcctattc cccttattcc aattttaggg
     4561 tgg
//
LOCUS       DAOOJC010000174         4546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_14947_length_4546_cov_3.735026, whole genome shotgun sequence.
ACCESSION   DAOOJC010000174 DAOOJC010000000
VERSION     DAOOJC010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14947_length_4546_cov_3.735026"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PLA01_05805"
     CDS             complement(<1..474)
                     /locus_tag="PLA01_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HPD00848.1"
                     /translation="MSLSRNLSITLICLILGVMLSWQYKSIQNNKKMASYQSGTLYTL
                     QEELLAEKTKNENLRARNQELEDQVSKYINAEGNNKKIEESLRDEIQRCRTLAGLNDV
                     VGPGVEIVIDDGEYSSVTDNYLLVFINEVRAAEAQAISINGERIVAMTEIKMTNDR"
     gene            complement(474..1184)
                     /locus_tag="PLA01_05810"
     CDS             complement(474..1184)
                     /locus_tag="PLA01_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HPD00849.1"
                     /translation="MKRKDIRFILMILFMLLGIVISVQFRSVLDANKDKPSIVYQIES
                     LRDQLNTEKIIGSELREQIDENMKKEEEYIKSIMNHSNDELIEEWKDARFIAGLSDVK
                     GNGVVIKISDAAKFEEGYIDDYVVHDTDLVVVLNELKKAGAQAISINNERIISTTEII
                     CAGPTVRVNRSRYAVPFEIKAIGNPKKLYEAIVNSPIAMEFREYGKVFEVREAKNIVI
                     PKFSGDLNNLVKDLEVVE"
     gene            1517..1834
                     /locus_tag="PLA01_05815"
     CDS             1517..1834
                     /locus_tag="PLA01_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00850.1"
                     /translation="MFYQIRYQTGEIEDVIKEMKNGKIPCMDVDNMAEFEWVVNKLKE
                     HGIYRISSIPLDRKARDMIKEPEFEFRAAFSAHEDGKGEPMYIDFYFEPIVEEDYDPI
                     FGD"
     gene            complement(1991..4453)
                     /locus_tag="PLA01_05820"
     CDS             complement(1991..4453)
                     /locus_tag="PLA01_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HPD00851.1"
                     /translation="MKAVIMAGGEGTRLRPLTCNRPKPMVPIVNKPVMEHIIELLKKY
                     ELTDVAVTLQYLPDMIKDYFGDGKEFGINLRYYVEDKPMGTAGSVKNAEKFLDDTFIV
                     ISGDALTDIDLKKAIDFHFSKGSIATLVLKKVDIPLEYGVVVTDENGRITRFLEKPSW
                     GEVFSDTVNTGIYILSPEVLRYFNKNEIFDFSKDLFPILLRENKPMYGYITDEYWCDI
                     GDLTAYSQAHKDVLDNKVKVTITGNKVRDGLWIGEGTVIEDNVTIEEPCIIGANTRIK
                     AGSVIGSYSVLGDNNIIGERSGIKRSIIWKNNVLDSNVQLRGTVICSKVNIKENVSTF
                     ENAVIGDDTVISSNVLVKPDIKIWPNKMVESGTEVSSNLVWGSKFIRSIFGFRGLAGE
                     INVDITPEYASKLGAAYGATFKGKGKIGISSDDSAPAKMLKMSFMSGLMSAGLKVLDF
                     GILHLPVFRSAVRFYGADGGIHIGTSSKDNSKLNVDFIDKNGSNIERGIERKIENAFQ
                     REDFSRCEGDAIDDIEVIPDYTGFYLRNILNNTKSKNMEYRVLLNSPSEYILETVESL
                     LTSLGCTVEKTVLDLQNAKAMSRGNDSGEMNYLISTARMGNFDMGVSIEDTSEKMMLV
                     DNNGRVISDDMFTALISLIMFKTVEGGTIVVPISASSVIEKIAELNNGRVIRTKTSPQ
                     DLMNKILGSEVKDNVYDQFALHFDAIAGLVKILDFMKSNNYKLSDLVDMIPQFYVSEK
                     EVDCSWNAKGKVIRQIIQENDNESIETLEGVKIFKDGGWVLVLPDAEQPVCRIKSESH
                     SAEFAEELTNFYVNRVKEISQR"
BASE COUNT         1259 a         1052 c          683 g         1552 t
ORIGIN      
        1 tctgtcattt gtcatcttaa tctcagtcat agccacaatg cgttccccgt ttatcgatat
       61 ggcttgagcc tccgccgcac gaacttcatt tatgaagacc agcaaataat tgtctgttac
      121 actgctgtac tccccatcat caataactat ctccactccc ggtcccacaa catcgttcaa
      181 acccgcaaga gtcctgcacc tttgaatttc gtcccttaag ctctcttcaa tctttttatt
      241 gtttccttca gcattgatat atttcgatac ctgatcctcc aattcttgat ttctggccct
      301 cagattctca ttttttgttt tttccgccaa gagctcctcc tgcaatgtgt acaacgttcc
      361 tgattgatat gaggccatct ttttattgtt ctggatgctt ttatattgcc atgacagcat
      421 aacccccaat atcaaacata ttaaagtaat ggacaaattt cttgaaagac tcattattca
      481 actacctcca gatctttaac taaattattc aaatctccac tgaacttcgg tataacaatg
      541 tttttagctt cccttacctc aaataccttt ccgtattctc tgaattccat ggcaatcgga
      601 ctgttgacaa ttgcctcgta gagttttttt gggttaccta ttgcctttat ttcaaaagga
      661 acagcatacc tgctcctgtt tactcgtacg gtaggtcctg cacatattat ctcggtagtg
      721 gaaattatcc tttcattatt gatcgaaata gcctgtgcac ctgctttttt caattcattg
      781 agcactacaa ccaaatctgt gtcatgaaca acatagtcat cgatatagcc ctcttcaaat
      841 ttagctgcat cactgatttt tataaccact ccatttccct tgacatctga aagacccgca
      901 atgaaccttg catctttcca ctcctctata agctcatcat tgctgtggtt cataatactt
      961 ttgatgtatt cctcttcttt tttcatgttc tcatcaatct gttcccttag ctcactacca
     1021 atgattttct cagtattgag ttggtccctt aagctttcaa tctgataaac gattgaaggc
     1081 ttatctttgt tggcatccaa aacactcctg aactgaaccg atataacaat tcccaaaagc
     1141 ataaacaaaa tcattaggat gaatcttata tctttcctct tcataatcaa gcttccttta
     1201 ttcataatag attcaaactg ctacttcgtg aaaatctgca aattcattaa atcgtataaa
     1261 ttaaaatacc ttgtaattta attctacaat aaaaaaatac tatttcaagt acatttatat
     1321 aaatgtttta tggatgctct ccctatcctt gtcgtattca caggctctgt acctgcgaaa
     1381 ctcaagttcc ttccatattt ttcttaacat gatttttatg tacatcgtat ataagttgct
     1441 tttataactc aattatgtgg tataaaatat atagtgaatt ttaatattcc gaatctattt
     1501 acctggaggt tattatatgt tttaccaaat cagatatcag accggagaaa ttgaagatgt
     1561 cataaaagaa atgaaaaacg gaaaaatacc ctgtatggat gtagataata tggctgaatt
     1621 cgaatgggtt gtgaataagc ttaaagagca tggtatttac cgcatttcaa gcattccttt
     1681 agacagaaaa gcaagagaca tgataaagga gcccgaattt gagtttcgtg ccgccttttc
     1741 agcccatgag gatgggaaag gagagcccat gtacattgat ttctattttg aacctatcgt
     1801 tgaagaggat tatgacccta ttttcgggga ttgacttggc aagaccgcaa tatttcaagt
     1861 ttgatttgag gttatgacct tattttatgg gattgcccct cttttttatc gggataattt
     1921 tactaattga gagggaaatt gaggggcaac atgccccacc tatttttctt aaacctgtcc
     1981 gattcaataa ttatctttgg ctaatctcct taaccctgtt tacataaaag tttgtaagtt
     2041 cttctgcgaa ctccgcagag tggctctcac tttttatccg gcacaccggt tgctccgcat
     2101 caggcaaaac cagtacccag cctccgtctt taaatatttt aacaccctca agggtttcaa
     2161 tgctttcatt atcgttttcc tgaataatct gccttattac cttgcctttc gcattccatg
     2221 agcagtcaac ctctttttca ctgacataga attgaggtat catatcaaca aggtcagata
     2281 gcttataatt attggatttc atgaaatcca gtatctttac caatccggca attgcgtcaa
     2341 aatgcagtgc aaactggtca tagacattgt cttttacttc gctgcctaaa attttattca
     2401 tcaggtcctg aggagatgtc tttgtcctta ttacccttcc attgtttagc tctgctattt
     2461 tttcaataac agaactagca gaaattggca ctacaatggt tcctccttcc acagtcttaa
     2521 acattatcaa agaaatgagg gcagtaaaca tatcatcgga aataacccgt ccgttgttgt
     2581 ctaccagcat cattttctcc gaagtgtctt caatggaaac acccatgtca aaattcccca
     2641 tccttgcggt gcttattaag tagttcattt cacccgaatc atttcctctg ctcatggctt
     2701 ttgcattctg caaatcgaga actgtttttt ccacagtaca cccaaggctt gtcaaaaggc
     2761 tttccaccgt ttcaagaatg tattcggaag gagaatttaa tagaaccctg tattccatat
     2821 tcttcgattt tgtattgttt aatatattcc ttaaataaaa cccggtataa tcgggaatta
     2881 cttcaatatc atcaatggca tctccttcac acctgctgaa atcctccctc tgaaaagcat
     2941 tctcaatctt tctttctata cctctttcta tattgcttcc gtttttgtct ataaagtcta
     3001 cgttaagctt ggaattgtcc ttgcttgagg taccgatatg aatacctccg tctgcaccgt
     3061 aaaaccttac cgccgaccgg aaaacaggca aatgcaggat tccgaaatca agaaccttca
     3121 gtcctgcaga catcaaaccg gacataaagg acatttttag cattttagcc ggtgccgaat
     3181 cgtcggagct tataccgatt tttcccttgc ctttaaatgt agctccatag gccgcaccaa
     3241 gctttgacgc atattccggg gttatatcca catttatttc tccagccaaa cccctaaatc
     3301 cgaatataga gcggatgaat tttgaacccc atacaaggtt tgagcttact tcagttccgc
     3361 tctctaccat tttattgggc catatcttaa tatcgggttt taccagcaca ttactgctaa
     3421 taaccgtatc atctccaatt actgcatttt caaaggttga gacattctcc ttgatattaa
     3481 ccttgctgca tatgacagtc ccccggagtt gtacattgct gtcaagaaca ttgtttttcc
     3541 atattatgct tctttttata ccgcttcttt caccgataat gttgttatcc cccaagacag
     3601 aataacttcc gataacagaa ccggctttta tccttgtgtt tgctccaatt atgcaaggct
     3661 cctctatagt gacattatcc tctataactg tcccttctcc aatccacaga ccgtctctta
     3721 ctttgtttcc tgtaattgtt actttcacct tgttgtccaa cacatcctta tgggcctggc
     3781 tatacgctgt caagtctcct atgtcgcacc agtactcatc ggttatatat ccatacatcg
     3841 gtttattctc cctgagaagt atgggaaaaa ggtccttgct gaaatcgaat atttcatttt
     3901 tgttaaaata cctcaaaacc tcaggagaca atatataaat tcctgtattt acagtatcac
     3961 tgaaaacctc tccccagctc ggtttttcca aaaacctcgt tatcctgcca ttctcatcgg
     4021 tcacaaccac tccgtattcc aatggtatgt caaccttttt caaaacaagt gtggcaatag
     4081 agcccttact gaaatgaaaa tctatagcct tttttaggtc aatatccgta agggcatcac
     4141 cgctaattac tataaatgtg tcgtcaagaa acttttctgc attttttacg cttcctgccg
     4201 tccccatcgg tttgtcttca acataatatc ttaagtttat accaaactcc ttaccatcac
     4261 caaaatagtc ttttatcata tccggtaagt actgcagcgt cacagcaaca tctgtaagct
     4321 cgtatttttt aagcaattca ataatatgtt ccataacagg cttgtttaca ataggtacca
     4381 taggtttcgg tctattgcac gtaagaggtc ttagtctggt accttctcct cccgccatga
     4441 taactgcttt catctaattc atcctttctt agaattcttg cataaacttc aaaaaactat
     4501 ttataatagt tataattaaa aacaaatttt taacatgtgg tattat
//
LOCUS       DAOOJC010000175         4513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15083_length_4513_cov_3.895693, whole genome shotgun sequence.
ACCESSION   DAOOJC010000175 DAOOJC010000000
VERSION     DAOOJC010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15083_length_4513_cov_3.895693"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PLA01_05825"
     CDS             complement(<1..379)
                     /locus_tag="PLA01_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00852.1"
                     /translation="MSTTYSVVFALIILNVVIVLLLILKANPLDLFRYFYAWLTGRLA
                     KINTKKTTERIKRKRIEKEKKGIISRYNNMVENLIEDYHLPLTLEGFNTVLTFLIIVA
                     VIFIFIFVKNVLLSILVSISLTVA"
     gene            complement(363..1808)
                     /locus_tag="PLA01_05830"
     CDS             complement(363..1808)
                     /locus_tag="PLA01_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPD00853.1"
                     /translation="MLTRSSVSEKQKKSQYYSASGSDAKYMAMSFENAVKQCQAYINK
                     VATHYYRQVEDVKTKREMTERYIIEYVDTEKPDVEGFSELLALKEALIEEITQYGPIT
                     DAMEDPEIDEIRANGPDQIFVERYGKSYRWDKSFADREHMEKVITKLIRASKVRLTPK
                     NPMVNARTIEGSRVNATHASISPYGNPAFVIRKFKKARLSAEEMIKEQSFSTNMYKLL
                     TLIPRANLSWLTVGPTGSGKTTLNEMLVKYIDPMCRIITIENPSELRLVRREGDTPTG
                     RIINDVLQYESISEDDADSSSATMENLLINAMRQSPHWIGPGELRAPREFTTALRAAQ
                     TGHYIFTTIHAEGDQEAIFRFLTAYLSASNEPAELALRNICAAIKFIVFQERLADGTR
                     KVTSISEVRGAKGLTPIIEQIYKFECIDVEEDPETGKVKRIVGKHKRVGKISQALEQK
                     MLKSGIKRSRFEFLTREVSPDEEEVYDVDNI"
     gene            complement(1843..2667)
                     /locus_tag="PLA01_05835"
     CDS             complement(1843..2667)
                     /locus_tag="PLA01_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPD00854.1"
                     /translation="MDSTRKLDIIGELGKLSPKEKLFEELESKKILENVIGFIPACDS
                     ADVPILVSNLGVYLAEMGYSICILDADVFYPCVYKLFGCESKPRGRGLLSVLRSDKMD
                     FRDEILKTKYKNVYIMSSSFMDPIEDYLDIRQRDFERVLLTLKDMFDLVLVSIPNNPP
                     LETCYVSVKNCDMGFVVWNERVDCALNTSKLMAFYSSINIGVAKFSNIIINNAVGLRF
                     DRDVIKEMNLKLVTELPFVPAAVDHSLMGEVYIAESTIINKRYKKSLEKLAQLIAN"
     gene            complement(2646..3518)
                     /locus_tag="PLA01_05840"
     CDS             complement(2646..3518)
                     /locus_tag="PLA01_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgA"
                     /protein_id="HPD00855.1"
                     /translation="MKINSIKDIGTGRRFPKRIFAVLISITIIVVAFVVLNNADNAAK
                     DTVDIVRIRPSDGIPANTLVTEDMIEKYPIIRKEYDEGMITYDKKDEVLNKYTLYYLR
                     SKSPVYSDQLTTEKPLKNEWLYELDDKNEVLTLPYNYLNCGGDILTPGDIVRIRVAYT
                     ETVTGSTDDYPGFSAQEEQRKLEVLFDRITVRDLLNSKGHSIYEVYKEVLKLGEEQRQ
                     KVMKSSEFMENILPRALILEGTKEQADKYAKFSDDGEARFTITILSRKDNKNIMDQLP
                     TIEKEVESWIQQGN"
     gene            complement(3593..>4513)
                     /locus_tag="PLA01_05845"
     CDS             complement(3593..>4513)
                     /locus_tag="PLA01_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00856.1"
                     /translation="FESTMLLGNNVDRSSKEFPGMMDQGKTVYPNFDNKPVNLSLTST
                     NINRFIAVTGHRGSGVTSTAANLAVEASVQGLKTIIIDFDIDYKAMNLYFSKFGEEVN
                     LNPELYNSLIKCLIKPESYEVNSCRINGNLLLTTLAYSIDNSDKMMQLINSKRLITLA
                     SVLKTRFNLVILDIPIDILREYDDLLIHLDSVALCINNSLHSVINTVCSMSKFERSSL
                     GILALKSKVIVTNYNERNLYDKKPLTSKVTCDILSELSGDVFSQNPECVGEIPYFPDF
                     DQQIDSGKKLCTVNDGFKGVYVNILNGLFK"
BASE COUNT         1244 a         1043 c          724 g         1502 t
ORIGIN      
        1 ctgcaaccgt taatgaaatt gataccagta ttgacagcaa tacatttttt acaaatataa
       61 atatgaaaat tacagcaact attatcaaaa aagtcaaaac agtattaaag ccttccaatg
      121 ttagagggag atgatagtcc tctatcaaat tctccaccat attgttatac ctgctgataa
      181 ttcctttctt ttctttctct atcctttttc tcttaattct ttctgtagtt ttcttcgtgt
      241 taatctttgc aagccttcca gtcaaccaag cgtaaaaata tctgaataaa tccagaggat
      301 tagcttttaa tataagaagt aatacaatga ccacatttaa tataattaaa gcaaacacta
      361 cactatatgt tgtcgacatc atatacctcc tcctcatcag gacttacttc tctagttaaa
      421 aattcaaacc ggctcctttt aataccggat tttaacattt tttgttccag agcttgagat
      481 atcttgccca ctcttttatg ctttcccact atccttttta cctttccggt ctcaggatct
      541 tcctcaacat caatacattc aaatttataa atctgttcaa taatcggagt cagtcccttt
      601 gcacctctta cctcagatat ggaagttact tttcttgttc cgtccgccag tctttcctga
      661 aagactataa actttattgc agcacaaata ttccgaagag caagctctgc cggctcattt
      721 gaagcactca ggtacgctgt cagaaacctg aaaattgctt cctggtcacc ttctgcatgt
      781 atggtggtaa aaatataatg gcctgtctga gctgcacgaa gtgctgtcgt aaattccctt
      841 ggtgccctca actcaccggg acctatccaa tggggagact gccgcatagc attaatcaac
      901 aggttttcca tagtggcgga gctgctgtct gcatcgtctt ccgatatcga ttcatattga
      961 agtacatcat ttattattct gccggtggga gtatccccct ctcgtctgac caaacgcaat
     1021 tcggacggat tctcaatagt aataattcgg cacatgggat ctatatattt tacaagcatt
     1081 tcattgagcg ttgtctttcc gcttccggta gggccgacag tcaaccatga caaatttgcc
     1141 cgtggaatta atgttaatag cttgtacatg tttgttgaaa aagactgttc ttttatcatt
     1201 tcttctgccg aaagccttgc tttcttgaac tttcttatta caaaagcagg attcccgtaa
     1261 ggcgaaatag aagcatgggt ggcatttacc cttgatccct caatcgttct tgcattaacc
     1321 atgggatttt tgggggtaag ccttactttt gaggctctta tcagtttggt tataaccttt
     1381 tccatgtgtt ctctgtcggc aaaggacttg tcccatctat agctttttcc gtacctttcc
     1441 acaaatatct gatccggacc gtttgccctt atttcatcaa tttccggatc ttccattgca
     1501 tcggtaatag gcccgtattg cgtaatttct tcaattagtg cttccttcaa cgccaacagt
     1561 tcactaaaac cttcgacatc gggtttttcc gtatcaacat actctattat gtaacgctcg
     1621 gtcatctcac gttttgtctt tacatcttcc acctgtctgt aatagtgggt agcaaccttg
     1681 ttaatatatg cctgacattg ttttaccgca ttttcaaacg acatggccat atattttgca
     1741 tcgctgccgg atgcagaata gtactgggat tttttctgtt tttcactgac actcgaccgt
     1801 gtgagcattc tctaccacct catttatatc tgaatatgat aatcagtttg caattaattg
     1861 agccagcttt tcaagagatt ttttatacct tttgtttata attgtacttt cagctatata
     1921 aacttccccc ataagagaat gatctacagc tgcaggcaca aaaggcaact cagtaaccaa
     1981 ttttaaattc atttctttaa ttacgtccct gtcaaatctc aaacccacag cattatttat
     2041 aataatattt gaaaatttgg ccaccccgat gttaatcgag ctgtaaaacg ccataagttt
     2101 gcttgtgttc aaagcacaat ccacccgttc gttccatacg acaaacccca tatcacagtt
     2161 ttttaccgat acataacagg tttcaagcgg cggattgttc ggtatcgaaa ccaaaaccaa
     2221 atcaaacatg tctttaagtg taagcaaaac cctttcaaaa tctctctgcc taatatccag
     2281 ataatcttct ataggatcca taaagctcga tgacataata taaacgtttt tatactttgt
     2341 ttttaatatt tcatccctaa agtccatctt gtcgcttcta aggacgctca gcagccctct
     2401 tcccctcggt tttgattcac aaccgaataa cttgtataca cagggataaa atacatcagc
     2461 atccaaaata catatgctat aacccatctc tgccagatat acccctaaat ttgaaaccag
     2521 gatgggaaca tccgcagaat cacaagccgg aatgaatcca atcacatttt ccagtatctt
     2581 tttgctttcc agttcttcga atagtttttc ttttggagat aattttccca actccccgat
     2641 tatgtctagt ttccttgttg aatccatgat tcaacctcct tttctattgt gggcagttga
     2701 tccattatat ttttattgtc ctttctgctc aaaatagtta tggtaaaccg tgcttctccg
     2761 tcatcactga attttgcata tttatctgcc tgttcctttg tcccttcgag aattaatgcc
     2821 cttggaagta tattctccat aaattcactg ctcttcataa ctttttgcct ttgttcttcg
     2881 cctagcttta aaacttcctt atacacttca tagattgaat gccccttgct gttaagcaaa
     2941 tcccgcacag taattctatc aaacagtacc tccagctttc tttgctcctc ctgggcggaa
     3001 aaaccgggat aatcatcggt gcttcccgta accgtctcag tatatgccac ccttattctg
     3061 acgatatcac cgggagtaag aatatctcca ccgcaattga ggtaattata gggaagtgtc
     3121 agtacctcat ttttatcatc aagttcgtat aaccactcgt ttttcaaagg tttctctgtg
     3181 gtcaattgat ctgaatacac gggtgattta cttcgcaaat aataaagagt atacttgttc
     3241 aagacttcat cctttttgtc atatgtaatc atgccttcat cgtactcttt acgtattatg
     3301 ggatatttct caatcatgtc ttcagtcacc agagtgtttg ccggaatacc gtccgacggt
     3361 cttatcctga ctatatcgac agtgtctttt gccgcattgt ctgcgttgtt tagtaccaca
     3421 aaggccacaa caattatagt tatgctgata agaactgcaa aaattctttt cgggaagcga
     3481 cggcccgtac caatatcctt tatactgttt attttcatgc taattccccc tgttctatga
     3541 tactgtcatt ggccggttgc aatcattggc atatgtaaag actcaaccgg aatcacttga
     3601 acaaaccgtt caatatattg acataaaccc ctttaaatcc gtcattaacc gtgcataatt
     3661 ttttacctga atcaatctgc tgatcaaaat ccgggaagta aggaatttct cccacacact
     3721 caggattctg gctgaataca tcaccgctca gttccgataa aatatcacaa gtcacctttg
     3781 atgtcagagg ttttttgtca taaaggtttc tttcattgta attcgttact atgaccttcg
     3841 attttaaggc caaaatgccc agagaactcc tttcaaactt gctcatactg catacggtat
     3901 ttataacaga atgaagactg ttattgatac acagggccac tgaatccaag tgtatcaata
     3961 aatcgtcata ctccctcagg atgtcaatgg gaatatccaa tataacaagg ttaaatcttg
     4021 tcttcagaac cgatgccaag gtaatgagcc gctttgaatt aataagctgc atcattttat
     4081 cgctgttgtc aatggaatag gccagagtcg tcaatagcag atttccgttg attctacagg
     4141 agttaacctc ataggactca ggctttataa ggcactttat cagcgagtta tagagctccg
     4201 ggttaagatt tacctcttcc ccgaatttgc tgaaatacaa attcatagcc ttgtaatcaa
     4261 tgtcaaagtc aattattatt gtcttcaacc cctgaacact ggcctccact gccaaatttg
     4321 ccgcagtact ggtaactccg gatcctctgt gccctgtcac tgcaataaaa cggttaatgt
     4381 ttgtggatgt caaagataaa ttaaccggct tattatcaaa attcggataa acagtcttgc
     4441 cttgatccat catccccgga aattcttttg agcttctgtc aacattattc cccagcaaca
     4501 tcgttgattc aaa
//
LOCUS       DAOOJC010000176         4491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15168_length_4491_cov_4.147430, whole genome shotgun sequence.
ACCESSION   DAOOJC010000176 DAOOJC010000000
VERSION     DAOOJC010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15168_length_4491_cov_4.147430"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            391..3039
                     /locus_tag="PLA01_05850"
     CDS             391..3039
                     /locus_tag="PLA01_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HPD00857.1"
                     /translation="MKKLVIGILAHVDAGKTTLSESMLYLSGKIRKLGRVDNKDAYLD
                     TNELERARGITIFSKQAVFEIDDTLITLLDTPGHVDFSAEMERTLQVLDYAILVISGA
                     DGVQGHTKTLWHLLDIHKIPVFLFVNKMDQKGTDKHKLIKEIKERLGDGCIEFGAAEE
                     ESFYEQLAMCDEIMMEEYLEKGRTGTRQISAAIKERKVFPCFFGSALKLEGIEKFMEG
                     IVKYADIPRYSDEFGAKIFKISRDEQGNRLTHMKLTGGKLKVRDVLTDGIWKEKVNQI
                     RICSGEKFEAVSEIDAGSVVAVTGLSKTRPGDGLGAEKASKPPILEPVLLYQIILPEG
                     YDPRVMIPKLRQIEEEEPELHIVWDEQLQEIQVQVMGEVQIEILQSIIKRRFGVEVAF
                     DSGRIVYKETIANTVEGVGHFEPLGHYAEVHLLLEPGERGSGLVFGVECNEDDLSKNW
                     QRLILTHLNEKTHKGVLTGSAITDMKITLVSGKAHNKHTEGGDFREATYRAVRQGLKE
                     AESVLLEPYYSFWLEVPEKMVGRAMTDIEKMHGTCEITQIKGETAVLTGSAPVVTMRN
                     YQKEVIAYTKGLGRLFCNLKGYEPCHNTQEVMELIGYDSERDVENPAGSVFCTHGSGF
                     LVSWDKVKEYMHVESYFQKKDDLSSSQDIRINSSKEMSISLEEIDRIINKTFYSNQGR
                     KSVWKRRKTAAESYYKPDANVKKEKETKEEYLLVDGYNIIYAWPELKKLADGNMDAAK
                     NKLLDSLSNYQWIRGCRIIVVFDAYRVEGHREEVLDYYNIHVVYTREAQTADQYIEKF
                     AYDNKKNYNITVATSDGLQQIIVRGEGCSLLSARELKAEIEASNERMKQEFKERNRRK
                     PNFLADALSQESKQQMEDLIKDEEDK"
     gene            complement(3042..3701)
                     /locus_tag="PLA01_05855"
     CDS             complement(3042..3701)
                     /locus_tag="PLA01_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPD00858.1"
                     /translation="MKKYLEILKKVKLFKGIEESNLMALLSCLSGKLQHYEKNEIVFM
                     SGESTNSFGIVLAGEIQIVQEDYYGNRSILAKTGVGNLFGESFACAQTKNLLVSVIAT
                     TESDLLFIDCRRLASPCANSCTFHSRLIQNMLHITAMKNISLMQKIEFITKRTTREKL
                     LAYLSSEAKKAGSNCFSIPFNRQELADYLSVDRSAMSAELGKLRDDGILKFNKNYFEL
                     L"
     gene            3830..>4491
                     /locus_tag="PLA01_05860"
     CDS             3830..>4491
                     /locus_tag="PLA01_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPD00859.1"
                     /translation="MIRKIIKIDEDKCTGCGLCAEACHEGAIGMVNGKAKLLRDDYCD
                     GLGDCLPVCPTGAITFEEREAAEYDEAAVEKNKLQKQMGNLPCGCPGTKMKSIHRENE
                     LKPVNTDVNTNTSQLMQWPVQIKLVPVNAPYFEDANLLIAADCTAYAYGDFHNRFIKN
                     RITLIGCPKLDDGDYSEKLTAIIKNNNIKSVTVVRMEVPCCGGIENAVKNALLNSGKF
                     IP"
BASE COUNT         1461 a          780 c         1096 g         1154 t
ORIGIN      
        1 tacacccctt aaatactttt cttttctcta tttttgtctg caagaaagaa accatgctgt
       61 tgaaaaacag atgcgaaatg gcacatgaaa tcacacattt tattgttatt gcaaatcgca
      121 cagcttatct gataattaaa ttttatcata ttcaaaatat aatgtcaatt cgcacttaat
      181 gcctgggtgg aagccttttt aatttacaaa acaagccctg ctatagtata ataagcttgc
      241 attgcatcgg tttgtcccat taatcgggaa ggttttagaa aaatccttgc atatcgtttt
      301 ctataaaata ctcgtatgta ataataactt gatattttga aaagagaagt tcaaatcgat
      361 gagaaacaag ctagaaatga gaagaagtat atgaaaaaat tggttatcgg aatactggca
      421 catgtggatg ccggtaagac aacattatca gagagtatgc tttatttgag cggcaaaata
      481 agaaaactgg gaagagtgga caataaagat gcctatttgg ataccaacga actggaaaga
      541 gctaggggta tcaccatttt ttctaagcaa gccgtttttg aaatagacga caccctaatt
      601 acactactgg atactccggg acatgtggat ttttccgctg aaatggagag gacccttcag
      661 gtgctggatt atgccatttt agtaataagc ggtgcggacg gagtgcaggg acatacaaaa
      721 accctgtggc acctccttga cattcataag ataccggtgt ttttgtttgt caataaaatg
      781 gatcagaagg ggacggacaa gcacaagtta attaaagaga taaaagagcg actgggggac
      841 ggctgcatcg aattcggagc agctgaggaa gaaagctttt acgagcaact ggccatgtgt
      901 gatgagataa tgatggaaga gtatctggaa aaaggacgta caggtacacg gcagattagt
      961 gcggctatta aggagcgtaa ggtatttccg tgctttttcg gttcggcctt aaaactggaa
     1021 ggaattgaga aatttatgga gggtatcgta aagtatgcag atatcccccg ctattccgat
     1081 gaattcggag caaaaatatt caaaatatca agggacgaac agggcaaccg tctcacgcat
     1141 atgaagctga cgggcggaaa actcaaggtg agagatgtat tgaccgacgg aatatggaaa
     1201 gagaaggtaa accaaatccg tatatgttca ggagagaaat ttgaggcagt aagtgagata
     1261 gatgcgggtt ctgtagttgc ggtaacggga cttagcaaga ccagaccggg agacggcctg
     1321 ggggctgaga aagcttcaaa gccacccata ttggaaccgg tgctgctcta tcaaataata
     1381 cttccggaag gctatgaccc aagggtaatg attccaaagc tgcgtcagat tgaagaggaa
     1441 gaaccggagc ttcatattgt atgggatgaa cagttacagg agatccaggt acaggttatg
     1501 ggcgaggtgc agattgaaat tctgcaaagc attataaaaa ggcgttttgg tgttgaggtg
     1561 gccttcgact ccggaaggat tgtgtataaa gagaccattg ccaatacagt tgaaggagta
     1621 ggacactttg aaccgttagg gcattatgcc gaggttcacc tgcttttgga gccgggtgag
     1681 aggggaagcg gtcttgtgtt cggggtggaa tgcaatgagg atgacttgag caaaaactgg
     1741 cagagactta tattgaccca tcttaatgag aaaacccata agggggtttt gacagggtca
     1801 gcgattacag atatgaaaat tacattagta tcgggaaaag cacacaataa gcatacggaa
     1861 ggaggcgatt tccgggaagc tacctaccgt gcggtacgcc aaggtctaaa ggaggcggag
     1921 tccgtattgc tggagcctta ttactccttt tggctggaag taccggagaa aatggtggga
     1981 agggctatga ctgatattga gaaaatgcac ggtacatgtg aaattactca gataaagggc
     2041 gaaaccgcag ttcttacggg cagtgcccct gtggttacca tgagaaatta tcagaaagaa
     2101 gtaattgcct ataccaaagg tctcggcagg ttgttttgca acctgaaagg gtatgaaccg
     2161 tgccataata cacaggaggt tatggaactg ataggatatg attcggaaag ggatgtggag
     2221 aatcctgcag ggtctgtgtt ttgtacccat ggttccggct ttttggtgag ttgggataag
     2281 gttaaggaat atatgcatgt cgagagctat tttcagaaaa aagatgattt gagttcatca
     2341 caggatatcc ggatcaattc ttcgaaagaa atgtcaataa gtttggaaga gattgaccgg
     2401 attataaata agactttcta ctcaaaccag ggaaggaagt ctgtctggaa aagacggaaa
     2461 acagcggcag aaagctatta taaacctgat gccaatgtca aaaaagaaaa ggaaacaaag
     2521 gaagagtacc ttcttgtgga tggatacaat atcatctatg catggcctga actgaaaaaa
     2581 ttagcagatg ggaatatgga tgctgcaaaa aataaattgc ttgattcttt aagtaattat
     2641 cagtggattc gcggatgccg gatcattgtt gtgtttgatg cttaccgtgt tgaggggcac
     2701 cgtgaggaag tattggatta ttataatatt catgtggttt atacaagaga ggcacagact
     2761 gcggatcagt atatcgaaaa gtttgcctat gacaataaga aaaactataa tataacggtt
     2821 gccacatcgg acggtttgca gcagataatt gtgaggggag aaggctgttc cctgttgtct
     2881 gcaagagaac tgaaggcaga aatcgaagca tccaatgaaa gaatgaaaca ggagtttaag
     2941 gagagaaaca gaaggaagcc caacttcttg gctgatgcat tgtctcagga atcaaagcag
     3001 caaatggaag acctgataaa agatgaggag gataagtagt gtcataataa ttcaaaatag
     3061 tttttgttaa actttagaat tccatcatct cttagcttgc caagctccgc cgacatcgca
     3121 ctccggtcca cggacaagta atcagcaagt tcctggcggt taaacggaat ggaaaagcaa
     3181 ttgcttccgg ctttttttgc ctcggaggaa agataggcca acagcttttc ccgggtggta
     3241 cgttttgtaa taaactcaat cttttgcatc aaggatatgt ttttcattgc agtaatatgt
     3301 aacatattct gaatcaagcg gctgtgaaag gtacacgaat ttgcacatgg agaggcaagc
     3361 ctccggcagt cgataaaaag gagatcgctt tcggttgttg caattacact gaccagaagg
     3421 ttttttgtct gggcacaggc aaaggattct ccaaacagat tgcctacacc tgttttggcc
     3481 agaatacttc tgttcccgta atagtcttct tgaacaattt gaatctctcc tgccaggaca
     3541 atgccaaagc tattggtgct ttcaccgctc ataaaaacaa tctcattttt ttcataatgt
     3601 tgaagctttc ccgacaagca ggacaataac gccataagat ttgattcttc aataccttta
     3661 aataatttaa ccttttttaa aatttccaaa tattttttca taaaatcctc tttttgttgt
     3721 atttccaaca gatttattaa tttcagtata gtaaacttaa gctataaagt caataaagca
     3781 aataaagaag gtgcaatatg cacccgataa aagaaaggat ggtaataata tgataagaaa
     3841 aataattaaa attgatgaag ataaatgcac aggctgcgga ttgtgtgcag aagcttgcca
     3901 tgaaggagcc ataggtatgg taaacggtaa ggcgaaactt cttagggatg attattgtga
     3961 cgggttggga gactgcctgc cggtttgtcc aacaggagcc atcacctttg aggaacgcga
     4021 agcggcagag tatgatgaag ctgccgtaga aaaaaacaag ctgcaaaaac agatgggtaa
     4081 tctgccatgc gggtgccctg gtaccaagat gaaatccata cacagggaaa atgagctcaa
     4141 gccggttaac accgatgtca atacaaacac aagccagctt atgcaatggc ctgtacaaat
     4201 caagcttgta ccggtcaatg ccccctattt tgaagatgca aacctgttga ttgctgccga
     4261 ctgtacggca tatgcctacg gagattttca caacaggttt ataaagaaca gaataacctt
     4321 gatcggctgc ccaaaactgg atgatggaga ttacagcgaa aagctgacgg caatcattaa
     4381 aaacaataac atcaaaagtg tcacagttgt gagaatggag gttccatgct gcggcggaat
     4441 cgagaatgca gtgaaaaacg cactattaaa cagtggaaaa ttcataccgt g
//
LOCUS       DAOOJC010000177         4471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15234_length_4471_cov_3.870924, whole genome shotgun sequence.
ACCESSION   DAOOJC010000177 DAOOJC010000000
VERSION     DAOOJC010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15234_length_4471_cov_3.870924"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="PLA01_05865"
     CDS             complement(<1..1124)
                     /locus_tag="PLA01_05865"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPD00860.1"
                     /translation="MEGEILKGKYRIERLLGQGAMGRVYLCRNIEMGNLWAVKHIYTR
                     DQKVHLLAEVEILKKLNHVSLPKIIDVMQDESGVYIVESFIEGTNLRDKLKKEGPFDE
                     ETVVEWAKQLCEALKYLHNMKPNPIIYRDMKPSNVIITDDNRAIIVDFGISKEHKGEN
                     TKDTVVAGTPDYAAPEQLIAGGSTDQRTDIYSLGVTMYQLLTGELPKYEAQSLRECRG
                     DISPEMDFIVQKCIKKNLSERYQSVEELKKDLDNIRDLKIISLKESFRNRVIISLSLV
                     MTIISLLMVYLGFSNLYRERNAFIDINPKSIVLSAQQQGDLIIEKNYPDGSKSNIKVS
                     ELYWNSSDTEIAVVKDGKIYAVNQGECVITGEYDDKFFKI"
     gene            complement(1152..1505)
                     /locus_tag="PLA01_05870"
     CDS             complement(1152..1505)
                     /locus_tag="PLA01_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00861.1"
                     /translation="MGNEYIYISIGLLTLVINIILVTAYIKLNYFNKKHRLSNGDNRV
                     EKKPKVQVKPNIADTMDVNDRNLSYGFDASQRSSIDQAHGQNNYADPGKYTVVLPQNP
                     SEGTQVLGNNDKENQ"
     gene            complement(1545..4421)
                     /locus_tag="PLA01_05875"
     CDS             complement(1545..4421)
                     /locus_tag="PLA01_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPD00862.1"
                     /translation="MRKIFAFFLTVAIILNSILVVSFAATYKTITISPGQSWEFVNKT
                     MTVAGISQKSSGKFDSAAYDASGSVSFVYYREEGSVYAAEGGRTVVTVPSSSRAIQFE
                     YDSEKLTVRQASAPAVRAFTLNPGSNYEFTNSNSKDERLRQAGEQPVDFVEYGSDGST
                     DYISVGEDGVMFVPTKGRLAVSVPANSSAVTLYAANDHFNKYMTYQEVSEPAVYKLEM
                     KPGETYEIKNNGAKDQYLRSKNKELYDYVMYKPNNSVYSSNIYYSGGEVIPAEYRLVL
                     TLLPTANKDVFYMPYKEYKANSISITKSSDPAVYKVDVQPSESMAFTNTGSSDIVVYA
                     SLDDYYEAAVYKEDKSLYYAYESERGIQSLSPGQRIVVTVPNIMDMVTFYMAYDVYKN
                     SLKIESSDEPALHKIVVLPGQSYEFKNIGQNNIRIYQSGQDKYKYESYSKSGSLLSSE
                     NSYSGNNSVFPGERLVVSVPSGSKAIAFYSGFEAYKNCIDPSIQPHIGFSISKQLEKA
                     ESKDEVLNVLTGSFALIPEDQKTSDKVKEFSVTFAEEAIKKISNEEISISDNEVEIDS
                     SLVKKQVEKAKEALPDVEKVLEKSGIEKNRDIRPSIKINVKPEKEDALDLVIGKDLSK
                     NVADIYSVDFSAGDVEISLETSGLEEELEEMNKLTISIDEEEQESAMSMQNIVLASSS
                     TDIGPISAVAANSSSKVYKIDFKSGNGSKVDTLNNNVGLKIPAASDQGDYNCVYMVDS
                     SGKTEAVGGLYNSDTKTLSVKTKKSGDYYVVENKKSFDDIKNKDATMIKAIEVMAAKG
                     IINGRDNGKFEPDAPINRSEFATLIVRAIYKYNSNAKANFSDVEMKAWFYAFIASAKN
                     EGIINGYPDNTFKPQNVINKQEIIAICSSVLNKEKGFYYPKDPDKFIDFADASSIPAW
                     AKNAVAMANREGIIVKRRDGKFVGDQPFTRGDAAVILYRLFNKL"
BASE COUNT         1174 a         1015 c          736 g         1546 t
ORIGIN      
        1 tcaattttaa aaaacttatc gtcatactca cccgttatta cacattcgcc ctggtttact
       61 gcgtatatct ttccgtcttt tacaacagcg atctctgtat ccgagctgtt ccaatagagc
      121 tcggatactt taatattgct cttagaaccg tcggggtagt tcttttcaat gatcagatct
      181 ccctgctgct gggccgagag cactatggat ttgggattta tgtcaataaa ggcatttctc
      241 tccctataca aattcgaaaa tccaaggtat accataagca gcgaaattat cgtcattaca
      301 agacttaagg atataataac cctattcctg aaactctctt tgagggatat tattttcaaa
      361 tcccttatat tgtcaagatc cttttttagc tcttcaacac tctgatacct ctctgagagg
      421 ttctttttta tacacttttg aacaataaag tccatttcgg gagaaatatc gcctctacac
      481 tccctgaggc tttgtgcctc gtacttgggc agttctcccg tcaaaagctg atacatggtg
      541 acacccaggc tataaatatc cgtcctttgg tcagtactgc cgcctgcaat cagctgttcg
      601 ggggcagcat aatcgggagt acctgcaaca accgtatcct tcgtgttttc acctttatgt
      661 tccttagaaa ttccaaaatc cacgataatt gcccggttgt catcggttat aattacgttg
      721 ctgggcttca tgtcgcggta tattatagga ttgggtttca tattgtgcag atatttcaaa
      781 gcctcacaaa gctgttttgc ccattcaaca acggtctctt catcgaaggg cccttccttt
      841 ttcagcttat ctctcaaatt ggtgccttcg atgaagcttt ccactatgta aactccgctc
      901 tcatcttgca ttacgtcaat tattttagga agacttacgt ggttcagctt ttttagaatc
      961 tccacctcag caagcaaatg tactttttgg tccctggtgt aaatatgctt aaccgcccac
     1021 aggtttccca tttctatatt cctgcacagg tatacccttc ccattgcccc ctgtccaaga
     1081 aggcgttcta tcctgtattt gccctttaga atttcacctt ccattttgtt ccctttccct
     1141 ttaagcacct attattgatt ttctttgtcg ttatttccta aaacctgtgt tccttccgat
     1201 gggttttgag gtagaactac agtgtatttg cccggatccg cataattatt ctggccatga
     1261 gcttgatcta ttgaactcct ttggcttgca tcaaatccgt aactaagatt tcgatcatta
     1321 acatccattg tatcggcaat attgggtttg acctgtacct ttggtttctt ctcaacccta
     1381 ttgtcaccgt ttgaaagcct gtgcttttta ttaaaataat tcagttttat ataagcagta
     1441 acaaggataa tgttaattac caatgttaaa agtccaatag atatatatat atactcatta
     1501 cccatttcgt tctacctccg tctttttaac cgtcaatttt tatattatag cttgttaaac
     1561 aacctataga gaattactgc agcatctcct ctggtaaagg gctgatcacc cacaaatttt
     1621 ccgtcccgtc tctttactat tattccttcc ctattggcca ttgcaacagc atttttcgcc
     1681 catgcaggaa tactgctcgc atcggcaaag tcaataaatt tatcaggatc cttcggatag
     1741 taaaaacctt tttccttgtt aagaacagaa gaacatattg ctataatttc ctgtttattt
     1801 attacgtttt ggggcttgaa ggtattatcc gggtaaccgt ttataattcc ctcattcttt
     1861 gcagatgcta tgaaggcata aaaccatgct ttcatttcaa catcgctgaa gttggctttc
     1921 gcattgctgt tatacttata aatggctctt actatcaatg tggcaaattc acttctgttg
     1981 atgggagcgt ccggttcaaa tttgccgtta tcccttccgt ttattattcc ctttgctgcc
     2041 ataacttcaa tagcttttat catggtagca tctttatttt tgatatcatc aaagcttttc
     2101 ttattttcaa caacgtaata atctccgctc tttttagtct tgacactcaa tgtcttggta
     2161 tcggaattat aaagtcctcc cacagcttcg gttttaccgc tggaatccac catatagaca
     2221 cagttgtaat cgccttgatc actggctgca gggatcttca atccaacgtt gttattaaga
     2281 gtatctacct tactaccgtt tccagatttg aaatcaatct tgtatacttt agaacttgaa
     2341 tttgctgcca cagctgatat aggacctata tcagtactgc ttgaagccaa gacaatgttc
     2401 tgcatggaca ttgctgattc ttgctcttct tcatctattg aaattgtcag tttgttcatc
     2461 tcttcaagct cttcctcaag gccggatgtt tccaaagaaa tttcaacatc cccggcactg
     2521 aaatcaacgc tgtatatatc tgccacattt ttggaaagat ctttgcctat aaccagatcc
     2581 aaggcatctt ctttttcagg cttaacatta atctttatag aaggcctgat atccctgttc
     2641 ttctcaatac cgcttttctc cagcactttt tccacgtcag gcaaagcttc ttttgccttt
     2701 tccacctgtt tttttactaa ggaactatct atctctacct cattatccga aattgaaatt
     2761 tcttcattgg atattttttt gatagcttct tcggcaaaag ttaccgaaaa ctcttttacc
     2821 ttgtcgctgg ttttttgatc ctccggtatc aatgcaaaag aaccggtcaa aacattcaga
     2881 acctcatcct tgctctctgc tttctccaac tgtttagata tggaaaatcc tatatgaggc
     2941 tgtatgctgg gatcaataca atttttatag gcctcaaaac cggaataaaa ggctatagct
     3001 ttagaacctg acggaacact tacaaccaat ctttcaccgg gaaatacgct attatttccg
     3061 gaatatgaat tttcgctgct taaaagtgat ccgcttttac tgtaactctc gtacttgtat
     3121 ttatcttgac ctgactgata aattcttata ttattctgtc ctatattttt aaattcatag
     3181 ctctgtccgg gtaaaacaac aattttatgt aatgcaggct catcgctgga ttctattttc
     3241 agtgaatttt tataaacatc ataagccatg taaaatgtaa ccatgtccat gatattcggt
     3301 accgttacca caattctctg acccggagaa aggctctgaa tgcctctttc ggattcatat
     3361 gcataatata atgatttatc ctccttgtat accgcagctt cataataatc gtccaaggat
     3421 gcataaacta caatatccga tgaacctgta tttgtaaacg ccatgctttc cgaaggttga
     3481 acgtccactt tatatacggc aggatcactg cttttggtta tggatattga attagccttg
     3541 tattctttat agggcatata gaaaacatct ttgtttgctg tcggaagcag tgttagcacc
     3601 aagcggtatt cggccggaat tacttctcct cccgaatagt aaatattgct cgaatatacg
     3661 gaattattcg gtttatacat tacataatca tataattcct tgttcttaga tcttaaatat
     3721 tggtcctttg ctccgttgtt ttttatttca taggtttctc ccggtttcat ttccaatttg
     3781 taaactgcag gctcactgac ttcctggtaa gtcatatact tattaaaatg atcattggct
     3841 gcatataatg ttactgcaga agagttggca ggaaccgaca ctgccaagcg gccctttgta
     3901 ggaacaaaca ttactccgtc ctcaccaacg gatatataat ctgtacttcc gtcacttccg
     3961 tattcaacaa aatccaccgg ttgttcccca gcctgtctta accgttcgtc ctttgaattg
     4021 gaattggtaa attcataatt tgaacccgga ttaagggtaa aagctctaac cgcaggagca
     4081 gaagcctggc ggactgtaag cttttcactg tcgtattcaa attggatggc acgggaagaa
     4141 ctcggcacag taactacagt acggccgcct tctgcagcgt aaacagagcc ttcttcccta
     4201 taatagacaa aactgacact cccgctggca tcataagcag cagaatcaaa tttaccggag
     4261 cttttctgag aaataccggc tactgtcatg gtcttattta caaactccca actttgtccc
     4321 gggctgattg ttattgtttt atatgtcgcc gcaaaactaa caaccaatat ggaattaagt
     4381 attattgcta cagtgagaaa aaatgcaaaa atttttctca aaataatcac tcctttttgt
     4441 ttttcatttt tcttttctca tactttcaat g
//
LOCUS       DAOOJC010000178         4452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15330_length_4452_cov_4.058904, whole genome shotgun sequence.
ACCESSION   DAOOJC010000178 DAOOJC010000000
VERSION     DAOOJC010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15330_length_4452_cov_4.058904"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /gene="metF"
                     /locus_tag="PLA01_05880"
     CDS             complement(<1..867)
                     /gene="metF"
                     /locus_tag="PLA01_05880"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HPD00863.1"
                     /translation="MNLADLFKKKKPVISFEIFPPKLDTPLESIYDTLEQFKELKPDF
                     ISVTYGAGGSAKDRTVEIASKIKNEYHIESMAHFTCVGHSKAEIDSLLENLHANNLEN
                     ILALRGDPPANQPDFDFSKNVYKYASELIGHIRNKSNFFCIAAAAYVEGHSDSKRLKD
                     DLIHLKQKVETGVDFLVTQLFFDNRLFYDFLDKTSAMGINCPITPGIMPIFKADQIKR
                     IASLCGASFPAKLVVLMDKYGDNPEDMRKAGIEYASTQIRDLIDNGVDGIHLLTMNRP
                     KSSRTILENIGLL"
     gene            1477..1815
                     /locus_tag="PLA01_05885"
     CDS             1477..1815
                     /locus_tag="PLA01_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00864.1"
                     /translation="MKLLLIVLNKTEKLDELLEKMMDKGITGATIFNSTGMARELAKH
                     SEDFPIFGTLRYLMDPERKESKTIFMVLKDEKVEVVKAVVREVIGDLSQPDTAVMFTL
                     PVLSAEGVEF"
     gene            1816..2988
                     /locus_tag="PLA01_05890"
     CDS             1816..2988
                     /locus_tag="PLA01_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPD00865.1"
                     /translation="MTISVLFDLALLLFSGLIFGRVVKLIKLPNVTGYLIAGLIIGPY
                     CLKLLSADLVTDLEIISEMALAFIAFSIGAEFKLSYLKEVGLTPVIIAVFEALTASAL
                     VTVVLSLFGFDLEIALLLGAIAAATAPAATIMVVKQYKAKGPVTQTLLSVVALDDAVA
                     LMAFGFAMAIVNTMQHPEQSSVLMSVVSPVIEIVGSIAVGFVLGMVFTIPLRFFKKDS
                     NRTIITVGFVFLGSSIATMLGLSSLLLCMCMGAMLVNVTKAGGSVFKLAENITPPLFL
                     MFFVVSGAELNVNILPQIGVIGILYVVFRVVGKALGAYIGAVIMKAPDVVRKFVGLTL
                     IPQAGVAIGLSLIAAQTLPEYGQKIRAVILCGTLIYELVGPVVTKIGLKKAGEIKD"
     gene            3055..3873
                     /gene="nagB"
                     /locus_tag="PLA01_05895"
     CDS             3055..3873
                     /gene="nagB"
                     /locus_tag="PLA01_05895"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HPD00866.1"
                     /translation="MDIIIRENYDEMSRYAAEVIAGFVKNKPDCVLGLATGGTPIGTY
                     KELIRMHKEGLDFSQVKTFNLDEYLGIGIDLSKPYEKDQSYARFMYEELFKNINIKKE
                     NIHIPDGMTKEPDKFCRWYEEEIKKAGGIDLQLLGIGGDGHWAFNEPGSSLGSRTRVQ
                     ALTKQTLDDNYESFYKKAGIKREDMPHFAITMGIGTILEARNILMIANGKKKAGVVAK
                     CLEGPVTSQITASAIQLHSGGITVVLDKDAASELKNLDHYIHVEKLKRSMGTVM"
     regulatory      4044..4201
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4316..>4452
                     /locus_tag="PLA01_05900"
     CDS             4316..>4452
                     /locus_tag="PLA01_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibD"
                     /protein_id="HPD00867.1"
                     /translation="MKGDQFLCNDEKFMERAIELAKMGWGRTNPNPLVGAVLVKDGRI
                     IA"
BASE COUNT         1312 a          720 c         1034 g         1386 t
ORIGIN      
        1 caacaatcca atgttttcaa gaatggttct gcttgacttt ggacggttca ttgttaaaag
       61 gtgtattccg tcaacaccgt tatcgattaa atcgcgtatt tgagtacttg catattcaat
      121 tccggcttta cgcatgtctt caggattgtc cccgtactta tccattaata caacaagctt
      181 tgcgggaaaa gatgcaccgc atagggacgc aatacgctta atctggtctg ctttaaatat
      241 gggcattata ccgggagtaa ttggacaatt tatacccatt gcagaagttt tgtcgagaaa
      301 atcataaaaa agcctgttgt caaagaacaa ctgggttacc agaaaatcaa caccggtttc
      361 caccttttgc ttaaggtgaa taaggtcgtc cttaaggcgt ttgctgtcac tatgtccttc
      421 cacatatgca gctgcggcta tgcaaaagaa attgcttttg tttctgatat gcccgatgag
      481 ttcactggca tatttatata catttttgct gaagtcaaaa tccggttggt ttgccggagg
      541 atctcccctt aaagcaagaa tgttttcaag attattggca tgaaggtttt caagaaggga
      601 gtctatttct gcttttgaat gtccaacgca agtaaaatgt gccatacttt ctatgtgata
      661 ttcatttttt attttagaag ctatttcgac ggttctatct tttgcacttc ctccggcacc
      721 gtatgtaaca ctgatgaaat ccggttttaa ttctttaaat tgttctagag tgtcgtatat
      781 ggactctaga ggggtatcaa gcttgggagg aaatatttca aaagatatga cgggtttctt
      841 ttttttaaac aaatcggcta gattcatttt gacctcctga accaaataat aaaattattg
      901 ggttgatatc gttttgcata aagatattat agcacaggtg ttaagacagt caagtttaca
      961 taaacgatgg gtgaattgaa tagatgtaaa gaaaaatatt tcagaaactt gagttacgca
     1021 agtccagggc ctgtaaatac tccgtggggt cgagcaaaac tcacttcgtt cagacaatgc
     1081 tctccctttc cacgtcgtat taacaggctc tacaacttgc ttcacaatgt ttcttccata
     1141 tttttcttca cttaaataat attcactcaa aaagcaatgt gtgaattgaa tagaggaata
     1201 aaaaatacta taaattttat ttttattgtg ctaaaataaa tatcggttta aaaaaataat
     1261 ttgcaaatta ctgtaatttg acccggtaac ttaatagcaa tgtaattagt atcctctgct
     1321 tgtaataagt agaggatata gatttgcctt taaaggcggg gagtatatct ccctactcat
     1381 tgaaacacct cggttcaaag aaaatttttc tgcagacgaa aacttttctt taaatcgagg
     1441 tgttaaaaac agcaattttg aatggagtga ttgtgtatga agttattgtt aattgtactt
     1501 aacaaaacgg aaaagctgga tgaactactg gaaaaaatga tggataaggg gattacgggg
     1561 gcaacaattt ttaacagcac aggtatggca agagaactgg caaagcatag tgaagacttt
     1621 cccatatttg gcacgctccg atatttaatg gatcccgaaa ggaaagaaag caaaacgatt
     1681 tttatggtac ttaaggatga gaaggttgaa gtggtaaaag ctgttgttag ggaagtaatt
     1741 ggagatttgt ctcagccgga tacggcagtg atgtttactt tgccggtgct ttccgctgag
     1801 ggggttgaat tttaaatgac aataagtgtt ttatttgatt tggcactatt actgttttca
     1861 ggtctaatct tcggccgggt agttaagctt attaagctcc caaatgtgac aggctatctg
     1921 attgccggtc taattatcgg accgtactgt ttaaaattgc tttctgctga tcttgttaca
     1981 gatcttgaaa ttatatccga aatggccctg gctttcattg ctttttctat tggagcggag
     2041 tttaagctgt cttatttgaa agaggtgggg ttgacaccgg ttattattgc tgtctttgaa
     2101 gccttaacag cttcagcctt ggtaacggta gtattgtccc tttttggttt tgaccttgag
     2161 atagcattgc ttttaggtgc tattgcggct gctaccgcac cggctgcaac tataatggtg
     2221 gtaaagcagt ataaggcaaa aggaccggtg acgcaaacgc tgcttagcgt ggttgcactt
     2281 gatgacgctg tagcactgat ggcttttggt tttgccatgg caatagttaa taccatgcag
     2341 catcctgaac agagttcggt gttaatgtct gttgtttcac cggttatcga aattgttggg
     2401 tcaatcgctg tgggatttgt acttggtatg gttttcacca ttcctcttcg ctttttcaaa
     2461 aaagattcca acaggacaat catcactgtg ggctttgttt ttttaggttc ttccattgcg
     2521 acaatgctgg gattgtcttc tcttctatta tgcatgtgca tgggagcgat gcttgtcaat
     2581 gttacaaaag cgggaggttc tgttttcaaa cttgctgaaa acattactcc tccgttattt
     2641 ttgatgtttt ttgttgtttc cggagctgaa ctcaatgtta acattttgcc ccaaattgga
     2701 gtcataggaa tcctttatgt ggttttcagg gtggtaggaa aagccttggg agcttatatc
     2761 ggtgcagtaa taatgaaagc tcctgacgtg gtaagaaaat tcgtcggatt aacgctgatt
     2821 ccacaggccg gtgtagctat cgggctttca ctgattgcag cacagaccct tccggaatac
     2881 ggacaaaaaa ttcgtgccgt aatactgtgc ggtaccctga tttatgaatt ggttggacca
     2941 gttgtaacaa aaatcggatt gaaaaaggca ggggaaatta aggattgaat tatttattaa
     3001 aggtatttaa ataaaaatga aatatataaa attaataatg aggagaagat ggatatggat
     3061 attatcatta gagaaaatta tgatgagatg agtaggtatg ccgcagaggt tatagccggt
     3121 tttgttaaaa acaaacccga ttgtgtactg ggtttggcaa cgggaggcac accgataggc
     3181 acttataagg aacttataag aatgcataag gaaggactgg atttttcaca ggttaagacc
     3241 ttcaatcttg acgagtatct tggtattgga atcgatttaa gtaaacccta tgaaaaggat
     3301 cagagctatg caaggtttat gtatgaggag ttatttaaga acatcaatat taaaaaagaa
     3361 aacatacata tacccgacgg tatgaccaag gagcctgata agttctgcag gtggtatgaa
     3421 gaggaaataa aaaaggccgg aggtattgat ttgcagctat tgggaatagg aggagacggg
     3481 cactgggcat ttaatgaacc ggggtcttcg ctgggttcga ggacgagggt tcaggcattg
     3541 accaagcaga cccttgacga taattacgaa agcttctata aaaaggccgg cataaagagg
     3601 gaggatatgc ctcatttcgc tattacaatg ggaatcggaa ctatccttga ggcaagaaac
     3661 atactgatga tcgcaaacgg aaaaaagaag gccggtgtag tggctaaatg cctggagggt
     3721 cctgtaactt ctcagattac agcatcggca atacagcttc acagtggagg aattactgtg
     3781 gtgctggata aggatgcggc ctcggaactt aaaaatctgg atcattatat tcatgtggaa
     3841 aaactgaaaa gaagcatggg gacggtgatg taacggtctc ttatggttaa ttttagtcaa
     3901 aatatttttt ggatgagttt tcttggaatt ttgtggttag aatcttcaaa atattgcttg
     3961 actgaaaatg taggctgtga tatatttaac ttaatttaga aaaggttaaa tgtattgaag
     4021 ttttacaaat aaatattgct tagatttatc ttcagggata ggtgagattc cttaccggcg
     4081 gtagacagac agtatctgtg agcccgcgag ccattaggca gatccggtca aagtccggag
     4141 ccgacagtat gatgtattcc gtttaatggg gtacattgag tctggatgga agaagataag
     4201 gttttgttca attatttgaa taaggctccc tgggaataaa tctcaaggga gttttttggt
     4261 ggatttttgc ggaaacagac aaacagaaat ttcggtgtgc tttaattttt gcgggatgaa
     4321 gggtgatcag tttttgtgta acgatgagaa gtttatggag agggccattg aattggcaaa
     4381 gatgggatgg ggaagaacca atcctaaccc tttggtggga gcggtccttg ttaaagacgg
     4441 aagaataatt gc
//
LOCUS       DAOOJC010000179         4452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15332_length_4452_cov_3.203093, whole genome shotgun sequence.
ACCESSION   DAOOJC010000179 DAOOJC010000000
VERSION     DAOOJC010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15332_length_4452_cov_3.203093"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLA01_05905"
     CDS             <1..324
                     /locus_tag="PLA01_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPD00868.1"
                     /translation="IAWAADKGIISGYGDGLFRPDDLITREQMAVMLRNYMQYKGISL
                     GAKEYVEFADDDKISSWAKDAVADMKRYGLIAGVGDNLYAPQDTANRASVGQIIMNFI
                     YAYIG"
     gene            444..584
                     /gene="scfA"
                     /locus_tag="PLA01_05910"
     CDS             444..584
                     /gene="scfA"
                     /locus_tag="PLA01_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HPD00869.1"
                     /translation="MKHIKVLNGSTLKDSLKKGGCGECQTSCQSACKTSCTVANQSCE
                     KK"
     gene            800..2146
                     /gene="scfB"
                     /locus_tag="PLA01_05915"
     CDS             800..2146
                     /gene="scfB"
                     /locus_tag="PLA01_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HPD00870.1"
                     /translation="MIHKFSLMGTNIVVDVNSGAVHVVDDISYDVLDYYQDYTAGEIK
                     EKLSLKYDAHDIDEALEELDSLKADGLLYFKDPYQDYISKNDRESVVKALCLHICHDC
                     NLRCKYCFASTGNFGGERTMMSTEVGKKAIDFLIEKSKNRRNLEVDFFGGEPLMNFDV
                     VKEIVEYARGKEKEHGKNFRFTLTTNGLLLNEENIKYINENMQNVVLSIDGRKEVNDK
                     MRCRVDGTGCYDSIIPKFKHVAQLRNQDNYYVRGTFTRENLDFSEDVIHLADEGFEQI
                     SVEPVVAAKGSGFDLREEDLPKLFDEYEKLAQEYVRRGREGKGFNFFHFMIDLTQGPC
                     IIKRLSGCGSGHEYLAITPEGDLYPCHQFVGDSKFKMGNVFDGALDTDIQKYFKNSNV
                     YTRKDCGSCWAKFYCSGGCAANAYQFNNDIDIPYRVGCELEKKRVECALWIKAQNI"
     gene            2171..2836
                     /locus_tag="PLA01_05920"
     CDS             2171..2836
                     /locus_tag="PLA01_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPD00871.1"
                     /translation="MGLITLKDIRHNEEVVSFLEIGDKQLAALGYTEHSFRHVGLVSK
                     TAGEILEKLGFDEREVELARIAGYLHDIGNAVNRVDHAHSGAILAYNILTKMGMSYAE
                     AAEIMLAIGNHDENTGAAVSNISAALVIADKSDVHRTRVRNNDIITFDIHDRVNYAVE
                     SSNVFIDREKNLVVLELCIDTKICPVMDYFEIFLVRMAMNRRAAEFLGLKFQLIINGT
                     YLL"
     gene            2968..4305
                     /gene="secD"
                     /locus_tag="PLA01_05925"
     CDS             2968..4305
                     /gene="secD"
                     /locus_tag="PLA01_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPD00872.1"
                     /translation="MKARSGIKCFLVIAVVAIVTAVVIAGFSVPGISFTMKRAYDQIR
                     YGIDIRGGVSAVLTAPEGVEPTDEQLDTVKGIIDKRLEAKQVFDKNVNVDRVNKRILV
                     EVPYKKDATSLNPYETMADLGAMAMLSFREVDEDKIDLETGAYIMTDRVVVQGSEIVE
                     ARAETNPQTQLPYVRLIFSDEGASKFEEATGRLVGKRIAIYLDEYMINAPIVNKQISG
                     KDNAVIELGVYERNQAMKEAKELAAVISAGALPFNLEAKDINAISPIIGESALTISVI
                     AGIVALILIWLFMIMFYRLPGFISCISLLGQMTGLILVIALTGMSLTLPGIAGIILTI
                     GMSVDANVIIFERIKEELRNGKTVQSSVDSGFKSALTAVFDGNITTLITAAVLYFIGT
                     GAVQSFAFTLGIGIILNFLTAVYATRIMLNTVSQIQGLRKPWLFGAKGGAANV"
BASE COUNT         1381 a          695 c         1123 g         1253 t
ORIGIN      
        1 atagcatggg ctgccgacaa aggcattatt tccggttatg gtgacggttt gttcagacct
       61 gacgatttaa ttacccgtga gcaaatggcg gtcatgctgc gtaattacat gcagtataaa
      121 gggattagtt tgggtgcaaa ggagtatgtt gaatttgccg atgatgataa aatcagttct
      181 tgggcaaagg atgcagttgc cgatatgaaa cggtacggat tgattgcagg cgtgggcgat
      241 aatttatatg ctcctcagga cactgcaaac agggcgagtg taggacagat aattatgaac
      301 tttatctatg catatatagg ataaaggcca cctggacaca atatttgcct attgaacggg
      361 aaagaaattt tatattaatc tttgcattgt aactgtttta tgctataata catcctgtac
      421 attacactat ggagggaaaa taaatgaaac atattaaggt tttaaacggt tcaacattaa
      481 aagatagctt gaaaaaaggc ggatgcggtg aatgtcagac ttcttgccag tcagcatgca
      541 agacttcctg tactgtagcc aatcaaagct gcgaaaagaa ataatccttt tacataatgg
      601 tattttgcca tagcagttta agctgtgctt tttataaagc aagaagatgg attttgtaat
      661 tcatatgata ttaatgaagt gaaaagtgaa aggaaatttc gcttttcact tttttagtag
      721 tatggtaatc aaaaacggga gcacagcaaa gggataattt caagaggatt catgtaaaag
      781 aggggaaagt ggaacagcaa tgatacataa gttttcgtta atgggtacca atattgttgt
      841 tgatgtcaac agtggtgctg tacatgttgt ggatgatata tcctatgatg tattggatta
      901 ttatcaggat tatactgccg gagaaattaa agaaaaatta tctttaaagt atgatgccca
      961 tgatatcgat gaggctttag aagagcttga cagcctgaaa gccgatggtc ttctttattt
     1021 taaagaccct tatcaggatt acatttcaaa aaacgatagg gaatctgtgg taaaggcgtt
     1081 gtgtcttcat atctgtcacg actgcaatct aagatgcaaa tactgttttg cctccaccgg
     1141 aaattttggc ggtgagagaa caatgatgag cacggaggtt ggaaaaaagg ccattgattt
     1201 tctcattgaa aagtcaaaaa accgccgaaa tcttgaggtg gatttttttg gcggggaacc
     1261 cctgatgaat tttgacgtgg taaaggaaat tgtggaatat gcccgaggca aagagaagga
     1321 acacggtaag aatttcaggt ttacattgac gacgaacggc ttgttattaa atgaagaaaa
     1381 tataaaatat ataaatgaga atatgcagaa tgttgttctc agtattgacg gccgcaaaga
     1441 agttaatgat aaaatgcgct gcagggttga cggtaccgga tgttatgaca gcataattcc
     1501 gaagttcaag catgtggcac agctgcgaaa ccaggacaac tactatgtga ggggaacctt
     1561 tacaagagaa aatttggatt tttcggagga tgttattcat ctggcggatg aggggtttga
     1621 gcagatatct gtagaaccgg tggtagcagc caagggcagc ggcttcgatc tgagggagga
     1681 agaccttccg aagctgtttg acgaatatga gaagcttgcc caagagtatg ttagaagagg
     1741 gagagaggga aaagggttta actttttcca ttttatgata gacctgaccc agggaccgtg
     1801 cataattaag aggctgagtg gatgcggctc gggacatgaa tacctcgcaa ttactcctga
     1861 gggagactta tacccctgcc accaatttgt gggcgacagt aagtttaaaa tgggcaatgt
     1921 atttgacggt gctttagata ctgatatcca aaaatatttt aagaattcca atgtatacac
     1981 cagaaaggac tgcggcagct gctgggcaaa attttattgc agcggcggat gtgcggcaaa
     2041 tgcataccag tttaacaatg acattgacat tccctacagg gttggctgtg aactggagaa
     2101 gaagagagtt gaatgtgcat tgtggataaa ggcacagaat atttagtttt catatagtgg
     2161 gagggctttt atgggactta taacgttaaa ggatataagg cacaacgaag aagtggtttc
     2221 ctttctggaa ataggtgata agcagcttgc tgctttggga tataccgaac actccttcag
     2281 gcatgtgggg cttgtatcga aaacagcagg agagattctc gaaaagctgg ggtttgacga
     2341 gagagaggtg gagcttgcca gaattgcggg ctatcttcat gatatcggaa atgcggtaaa
     2401 ccgggtggat catgcccatt caggggctat tttggcttac aatattttga cgaagatggg
     2461 tatgagctat gctgaggctg cggagattat gctggccatc ggcaatcacg acgagaacac
     2521 cggggcggcc gtaagcaaca tttcggcggc tttggttatt gcggacaagt ccgatgttca
     2581 cagaacccgg gtcagaaaca atgatatcat aacctttgat attcacgaca gggtaaacta
     2641 tgcggtcgaa agctccaatg tgttcattga ccgggaaaaa aaccttgttg tactggagct
     2701 ttgtattgat acaaaaattt gtcctgtcat ggattatttt gagatatttt tggtcagaat
     2761 ggctatgaac agacgggcgg cagagttttt aggacttaaa ttccaactca ttattaatgg
     2821 aacatattta ttgtagataa gctttttgaa ccgcggaaag attactgagc gggtgtttaa
     2881 taagaaaaaa tagtatattg acgtcaattt attatagtta tataatatcc acatgtggac
     2941 aagattgaaa aagggggaat tttacagatg aaagcacgca gcgggattaa atgcttcctg
     3001 gttattgcag tagttgctat tgtgactgcc gttgtaatag ctggtttttc agttccggga
     3061 ataagtttta caatgaaaag ggcgtatgac caaataaggt atggtatcga cataaggggt
     3121 ggagtaagtg cagttcttac agcacccgaa ggtgtggaac ctacggatga acagttggat
     3181 accgtaaagg gaataataga taaaagattg gaagcaaaac aggtttttga taaaaatgta
     3241 aatgtagata gagttaacaa aaggattttg gtagaagttc catataagaa agacgccaca
     3301 agccttaatc cttacgagac aatggcggat ttgggtgcta tggctatgtt gtctttcaga
     3361 gaggttgatg aggacaagat tgatcttgaa accggggctt atatcatgac cgacagggta
     3421 gtggttcagg gcagtgaaat agtagaagcc agagcggaga caaatcctca aacccagtta
     3481 ccttacgtaa ggctgatatt tagcgatgag ggagccagta agtttgagga agcaaccgga
     3541 agacttgttg gaaagaggat tgccatatat cttgatgaat atatgataaa tgcaccgatt
     3601 gtcaataaac agatatccgg taaagataat gcggttattg aactgggagt gtacgaacgc
     3661 aaccaggcta tgaaagaagc aaaagagctg gcagcagtta tcagtgccgg ggcgttgcct
     3721 tttaaccttg aggcaaaaga tatcaatgcc attagtccaa tcatcggaga aagtgcactt
     3781 acaataagtg taattgccgg aatagttgca ttgattctta tctggctgtt tatgattatg
     3841 ttttacagac ttcccggttt tatttcatgc atatcccttt tggggcagat gacgggtttg
     3901 atactggtga ttgcattaac tggtatgtcc cttactcttc ctggtatagc gggtataatt
     3961 cttaccatag gtatgagtgt tgacgcaaat gttataatat tcgaaagaat taaagaagag
     4021 cttagaaacg gaaagactgt ccagtcttcc gtcgattccg gatttaaatc ggcattaact
     4081 gcggttttcg acggtaacat aaccacattg attaccgctg cagtgctcta ttttatcgga
     4141 accggtgcag tacagtcctt tgccttcacg cttggtatag gtataatatt aaacttttta
     4201 actgctgttt atgctacaag aattatgcta aatacggtat cacaaataca gggattaaga
     4261 aagccgtggt tgtttggtgc taaaggaggt gctgcaaatg tttgattttg tctcaaagaa
     4321 aaaatataca atgatattgt ccgctttgat tatattggca ggtattgtag caatggtagt
     4381 tagaggaggt cttaaccttg atgtgcagtt ccgtggagga actcgaatag agattgaaat
     4441 ggagaacggg ga
//
LOCUS       DAOOJC010000180         4447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15354_length_4447_cov_2.882058, whole genome shotgun sequence.
ACCESSION   DAOOJC010000180 DAOOJC010000000
VERSION     DAOOJC010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15354_length_4447_cov_2.882058"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="PLA01_05930"
     CDS             <1..1154
                     /locus_tag="PLA01_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00873.1"
                     /translation="ENDISYMIKDSITIAEIGFKVLRMPIDVQSTFSINADECKINSF
                     SCKLIGGIEFCSNIDYKDNAINNSFVPDNNQKYEETVTDKSVSSKANTRKSKLNYILG
                     LSLLVSVFILSALAFNSIRKKKTPIIPNSNTATISQSPKGQEAPTPLNTIAPAVEASI
                     PPSAAPTLTKNPASTEPTQSDFPGQFHTPYITETPSTPINTVLTPTKTPIPTPTHTPV
                     PTNTPMSSNILAPVKITELLYPANWAVAGKSNAVIGIESGQISSRNALSINYSITGNG
                     NASIKTRENSIEFPEGRNKLVFAYRCLSQNSLRDSITIIINYSDNKTERINLGSSEEI
                     SSLKNWATKEITLNGISKVNSIEIFIGNLFNQSTETNEGVLMLSDFYMY"
     gene            complement(1158..4178)
                     /locus_tag="PLA01_05935"
     CDS             complement(1158..4178)
                     /locus_tag="PLA01_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPD00874.1"
                     /translation="MTRKIISSLIVIALFLTIILAAVSNEVYGEIKWLKVSAGDSYSF
                     ALDETGDLWAWGSNCYGQLGDGTTENSSRPALVMKGVMEVVGGQYHSFAVKKDGTLLV
                     FGEMFTMQGYGKSKGEPVAIMDKVKTAAIGRNFWIVVKEDGSLWSWGDNRYGQLGDGT
                     NNNRTVPKKVMDGVEKVTTGLNHTLALKKDGSLWMWGSGVNQPEKVLSDVVEIASGAN
                     HNLAIKEDGSLWAWGSNTSGQLGDGSNRNYYVSPIEIMKGVKKVAAGYDYSLAVKEDG
                     SLWTWGGNNYGQLGNGTTEKVKKPEKITDGVIMITAGVNHSLAIKKDGSLWAWGSNSQ
                     GQLGREEAYRVLPSQIMQGVVKVASGGGGWGYSLVIRSDKSLWACGYNMNGQLGDGTT
                     DNKNKHVRIIDSGVKKVSANGHSFAINEDGDLWAWGNNSYGQLGDGTNIDKSRPIFIM
                     SEVVEVSAGTSHTLAVKADGSLWAWGSNEMGQLGDGTTIQSIYPKKIMDSGVKKAIAG
                     NKFSLAVKEDGSLWAWGWNAYGQLGDGTKEDSHYPKKIIGEGVVNAHAGFSHSLAIKE
                     DGSLWAWGRNRCGQLGDGTTTDRKVPVEIEQIYNVVDVVAGTDYTFALKADGSLWGWG
                     YNSNGQLGIGTKATIEYLPVKVMEGVINISSSGGSTFAVKSDKSLWAWGSNSFGQLGD
                     GNAYKELPQRVVFDSEPTKPTATTNITPSPSIPAPTASNTPKVTTVPEPTTSPDATKT
                     PVVTKTPGHEETPKPPDIPIYPTPSKNTGNSTDNKDNTPLPPTPTQTMQAIQELPEII
                     EPTPDNPGVGFSDINKHWAQESIIKLLERDLIVGYEDNTIRPDQYITRNEAIVLLAKL
                     LKLEPRDDIALAFSDKDRIPEWVKPYLKISQNYGLIVGFEDNSFRGQNNLTRAQASVV
                     FLKVLSLTGGIDKSISEISGIPGAYKINSAIVEFKDFTKSHWSYAYLDMAFSIELIKG
                     YSDKTIRPDNSITRAEFFTLAAKVLELSETKRVR"
BASE COUNT         1217 a         1141 c          709 g         1380 t
ORIGIN      
        1 gtgagaatga tatctcttat atgataaagg acagtatcac aattgcagaa ataggtttta
       61 aagtgttaag aatgccaatt gatgttcaaa gcactttttc tattaatgca gatgaatgca
      121 aaatcaacag cttttcctgt aaactcatag gaggaataga attctgctcc aatatagact
      181 acaaggacaa tgcaataaat aattcttttg taccagataa taatcaaaag tatgaggaaa
      241 ccgtcacgga caaatccgtc agcagtaagg caaatacaag aaaaagcaaa ttaaactaca
      301 tattagggct ttccctctta gtatccgtat ttattttatc tgcactggct tttaattcaa
      361 ttagaaagaa aaaaacacct ataattccga actcaaacac agctacaata tcacaatctc
      421 caaaaggtca agaagcccca actccattaa acactatagc acccgcagtt gaagcttcaa
      481 ttccaccgtc cgccgctccg acattaacaa aaaatccggc atccacggag ccaactcaaa
      541 gtgattttcc cggtcaattc catacacctt acataacaga gacgccttca acacctatca
      601 atacggtttt gacccctaca aaaacaccaa tacctactcc aacacataca cccgtaccta
      661 caaacacccc tatgtcttcg aatatactcg cacctgtaaa aattacagaa ttactgtatc
      721 ctgcaaattg ggctgttgcc ggaaagagca atgcagtaat cggcattgaa agcggacaaa
      781 taagctctag gaatgcactg tccataaact atagtataac cggtaacgga aatgcctcca
      841 taaaaacaag agaaaactct atagagtttc ctgaaggccg taacaaactg gtatttgcat
      901 atagatgctt atctcaaaac agtcttaggg acagtatcac aattatcatt aactactctg
      961 acaataaaac ggagcgaata aatcttggca gttcggaaga aatatcaagc ctcaagaatt
     1021 gggctaccaa agaaatcact ctcaacggca tcagtaaagt caactcaata gaaatattca
     1081 tcggtaattt atttaaccaa agcactgaaa caaacgaagg tgtactcatg ctttccgact
     1141 tttatatgta ttaatattta ccttacccgt ttagtctcag agagctccag tacctttgca
     1201 gccagagtga aaaactcagc tctcgttata ctgttgtccg gtctgatggt tttatccgaa
     1261 taccctttta tcaactctat tgaaaatgcc atatccagat atgcataaga ccagtgggat
     1321 tttgtaaaat ccttgaactc aactatggca ctatttattt tgtaggcccc aggtattcca
     1381 gaaatctccg aaatactttt atctatacct ccggtgaggg ataagacctt taagaagaca
     1441 actgaggcct gtgctcttgt aagattattc tgtcctctga aactgttatc ctcaaatccc
     1501 acaattaagc catagttctg agaaatcttc aagtaaggct taacccattc cggtatcctg
     1561 tccttgtcac taaaggccag agcaatatcg tctctcggct ccaattttaa tagtttggcc
     1621 aataatacta tagcttcatt tcgtgttata tattggtccg gtcttatcgt gttgtcttca
     1681 taacctacta ttaaatcccg ctcaagcagc ttgataatcg actcttgtgc ccaatgttta
     1741 tttatgtcgc tgaaaccaac tccaggatta tccggtgtag gttcaattat ctccggtaac
     1801 tcctgtatcg cctgcattgt ctgagttggt gtcggcggca atggggtatt gtctttgtta
     1861 tctgttgaat ttcccgtatt ctttgacggt gtcgggtaaa tcggtatatc cggaggcttt
     1921 ggagtttctt catgtccagg agttttcgta acaaccggtg tctttgtggc atctggtgaa
     1981 gtcgtaggtt ccggcaccgt tgtgaccttc ggtgtattcg aagcagttgg agcaggtata
     2041 ctaggggatg gtgtaatatt cgttgttgct gtcggttttg tcggttcgga atcaaatacc
     2101 actctttgag gaagctcttt gtaagcattc ccatcaccca actgtccaaa agagttgctg
     2161 ccccacgccc ataagctttt atcgctcttt accgcaaaag tacttcctcc acttgaagaa
     2221 atgtttatta cgccctccat tacctttacc ggtaaatatt caatcgttgc tttcgtccca
     2281 attcccaact gaccgttgct gttgtacccc cagccccata agcttccatc cgctttcagt
     2341 gcaaaggtgt aatccgttcc tgctaccaca tcaaccacat tatatatttg ttctatttct
     2401 acaggtacct ttctgtccgt tgtcgttccg tctcccaact gtccgcatct gttcctcccc
     2461 catgcccaca ggctcccgtc ttcctttatt gcaagactgt gagaaaatcc cgcgtgggca
     2521 tttaccactc cttccccgat tattttcttg gggtaatggc tgtcttcttt tgttccgtca
     2581 cccaattgac cataggcatt ccatccccac gcccataagc tcccgtcttc tttcactgcc
     2641 aaactgaatt tgttcccggc aatggctttt ttaactccac tgtccattat ttttttggga
     2701 taaatactct gtatagttgt tccatcccct agttgtccca tttcattact tccccatgcc
     2761 caaaggcttc catccgcttt cacagcgagg gtatgggaag ttcctgccga gacttcaacc
     2821 acttcactca taataaatat aggcctgctc ttatctatgt ttgtcccgtc tcctaactgt
     2881 ccataagagt tattccccca tgcccacagg tccccatctt catttattgc aaaactatgt
     2941 ccgttagccg atactttctt aaccccactg tcaattatac gtacatgttt gtttttatta
     3001 tcagtagtac cgtctccaag ctgcccattc atattgtaac cgcatgccca caaactttta
     3061 tcgcttctta tcacaagact gtatccccat cctcctcctc cggatgctac tttaactact
     3121 ccctgcatta tttgacttgg cagtacccta tacgcttctt ctcttcccaa ctgtccctga
     3181 gaattgcttc cccatgccca caaactaccg tcctttttta ttgcaaggct gtggtttaca
     3241 ccggcagtaa tcattattac tccatctgtt attttttcag gcttcttcac cttttctgtc
     3301 gttccgtttc ccaattgtcc gtagttattg ccaccccatg tccacaaact tccatcctct
     3361 tttaccgcaa gactgtagtc atatcctgcc gcaactttct taaccccttt cattatctct
     3421 atcggactta cgtaatagtt tctgttcgac ccatctccca attgtccgga agtgttgctg
     3481 ccccatgccc acaggctgcc atcttctttt attgcaagat tgtgatttgc acctgacgca
     3541 atttcaacaa catcggataa tactttttcc ggttggttta caccacttcc ccacatccat
     3601 aaactgccgt cttttttcag tgcaagggtg tggttcaacc cggtcgtcac tttctcaact
     3661 ccatccatca ctttttttgg tacagttcta ttattatttg tcccgtctcc caactgtccg
     3721 tatctgttat ctccccatga ccacaggctc ccgtcttcct tgacaacaat ccagaaattc
     3781 cttccgatag ctgcggtttt tactttgtcc attatcgcaa caggttctcc cttgcttttc
     3841 ccataaccct gcatagtaaa catttcgcca aataccaata gggttccgtc tttctttacg
     3901 gcaaaactat ggtactgtcc tccgacaacc tccataactc ccttcataac cagagccggc
     3961 cgggagctgt tttcggttgt gccgtctccc aactgtccat agcagttact tccccatgcc
     4021 cacaaatctc ccgtttcatc caaggcaaag gaataggaat caccagcaga aaccttcagc
     4081 cattttattt ccccatatac ctcattagaa actgccgcta atataattgt caagaaaaga
     4141 gcaatgacaa ttaaactcga aattatcttc cttgtcattc gaatcctccc caaaatcagt
     4201 atttatatag gctctataaa actgcaataa cataaaagcc tataattctg cgatttatag
     4261 cattaaagac atttcaaact gcaaaatgta aaataagttt tgcattgtcc atatacataa
     4321 aaattatatc acatacagga tgcttccaca gccatctaaa catattaatt taataaattt
     4381 attgaaaata taaaaaatag gcggctgcaa ctttcttttc aatgcttttt cacttttctt
     4441 cataaaa
//
LOCUS       DAOOJC010000181         4440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15380_length_4440_cov_3.741847, whole genome shotgun sequence.
ACCESSION   DAOOJC010000181 DAOOJC010000000
VERSION     DAOOJC010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15380_length_4440_cov_3.741847"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLA01_05940"
     CDS             <1..330
                     /locus_tag="PLA01_05940"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPD00875.1"
                     /translation="VSVGATATAENFLNIKTISIILLGLAAFSIGTASGVLFGKLMYY
                     MTGGKVNPLIGSAGVSAVPMAARVSQKVGQEANPSNFLLMHAMGPNVAGVIGSAIAAG
                     VLLSIFR"
     gene            complement(490..1383)
                     /locus_tag="PLA01_05945"
     CDS             complement(490..1383)
                     /locus_tag="PLA01_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPD00876.1"
                     /translation="MINGFKVVDGHVHTFMSDEVSLKIRTSFNKVYSIEFENPGTGTV
                     DDVLKNMKGLGIDFTVMANFAPAKILDKNNRWTLDASKQHPALIPLVSFHPDMEGDFE
                     RLLEEYIEEGARGVKLHPMAQGFDPRNEKLKGMYGVCNEIAFPVVFHCGRVANARLNE
                     YSDVDTIEPIIKMYRNIPFILTHMADGSVKDVLRLSQQYENVFFDTSIVITGFPPILQ
                     TNEPSWVEDDIVVEVVEKVGAERLVFGSDYPWGSPGHDLKRFLSMQISDEQKRLILGQ
                     NAVRLFVKKDYMLRNLDTASF"
     gene            1635..2843
                     /locus_tag="PLA01_05950"
     CDS             1635..2843
                     /locus_tag="PLA01_05950"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HPD00877.1"
                     /translation="MKYVLILGDGMADYPIQQLDNKTPLQYAKKNNIDFLAQHSEVGM
                     VKTIPDGIPPGSDAANLSVMGYNPREYYTGRSPLEAVSMGINLSDTDVAIRCNLVTLS
                     EDEDYTQKTMIDYSSDEISTEEARELINDVNRHLKSQDINFYPGISYRHCMVWNNGLT
                     GLNLTPPHDILEKKITDYLPKGEITKLLLDMMVKSYDILKDHPVNKARISRGLRPANS
                     IWLWGEGKKPLLSKYYDRYKINGSVISAVDLIKGIGILAGLKNVDVEGATGNIHTNFI
                     GKAQAALKELESGQDFVYLHFEAPDECGHRFEIENKVKSIEIIDEQVVGTILKGLEKY
                     EDYKLLILPDHPTPLSLRTHTPDPVPYLLYQKSKPKKSGVTGYDEVQAASTNIFFNEG
                     YKLMDHFILD"
     gene            complement(3031..>4440)
                     /locus_tag="PLA01_05955"
     CDS             complement(3031..>4440)
                     /locus_tag="PLA01_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HPD00878.1"
                     /translation="RKSIFSAIHATVSGAGFERKGIEELKRDVSRGEIDLLIIKDLSR
                     LGRNNAKTLLFLDFLEEKGVRVITFDGRYDSIRDNDTVGIDTWYNERYVRDISRKIRT
                     NLRFKIQKGEYIGNAPYGYEKSQHEKNKLVVNKEQAETVKRIYRLYREGFGYSYIAVK
                     LNQDGVKSPSMGLWNAVGVMRILSNRVYMGDTVQGVSEKISFKSKKTRRLPQSCWVVT
                     ENTHEPIIPKEEFYEVQRIRDEKRNKAGPHKGVLHVFRGMMFCGACGSIMFARKRQGR
                     PMSYICSGYGKNGKSACTSHHIRERELCSIVLNDLEELLNDSEATEQILRKILQDRGT
                     EDWRTVYKRLYKQLEAKQKQQEILYQDRLDEKISDSLFLRMNQQLESKISFIKKEMET
                     LETRKTESCNTVGIIQQFRDSLKTNGISNEIAKIMINRIVVFDKGDTYDESRWRLNLT
                     EEEKECIKLYGAVFIEYNF"
BASE COUNT         1262 a         1011 c          817 g         1350 t
ORIGIN      
        1 gtatcggtag gtgctaccgc cacagcagaa aacttcctca atataaaaac cattagcatt
       61 atacttcttg gtcttgctgc cttctcaata ggcacagcaa gtggtgtgct ttttggtaaa
      121 cttatgtatt atatgaccgg cggaaaagta aatcccctta tcggatcagc cggggtatca
      181 gcggttccta tggccgcaag ggtttcccaa aaggtcggtc aggaagcaaa cccctccaac
      241 ttcctattaa tgcatgccat gggaccgaat gtggcgggag ttatcggctc tgcgattgca
      301 gccggtgttc ttttgagcat cttcagataa catatggcag tgttttttaa caaaacgtac
      361 ttctaataat tagaatgata aatgtagaat ttcgataaaa agagaagagg tcttaaactc
      421 ttctcttttt atttgttatt tatcaaacct cacccgacat ttcttttcaa aagcatttca
      481 caacagtttt taaaaggagg cagtatcaag atttcttaac atgtaatctt tcttcacaaa
      541 cagcctcacg gcattttgac ctagaatcag tcgcttttgt tcgtcactta tctgcatgct
      601 taagaacctc tttagatcat gtccgggact gccccacgga taatcggagc cgaataccag
      661 cctttctgcc ccaacttttt cgactacctc cacaacaata tcatcctcaa cccaactggg
      721 ctcattggtt tggaggattg gagggaatcc ggttataaca attgacgtat caaagaagac
      781 gttttcatat tgctgcgaaa gcctcaagac atccttcaca ctgccatccg ccatatgggt
      841 aagaataaaa ggaatgtttc tgtacatctt gataatcggc tcaatagtat ccacatcgga
      901 atactcgtta agccttgcat ttgctaccct cccgcaatgg aaaactaccg gaaaagcaat
      961 ttcattgcaa accccataca tgcctttcag tttttcattc ctaggatcaa atccctgagc
     1021 catgggatgc agcttgacac ccctcgcccc ttcttctata tactcttcaa gcaggcgttc
     1081 aaagtctccc tccatatcgg gatgaaaact aaccagcggt ataagggccg gatgttgttt
     1141 cgatgcatcg agggtccacc tgttgttttt atccagtatt ttggccggtg caaagttcgc
     1201 cataacggtg aagtctatgc caagaccttt catattcttt aatacatcgt caacggttcc
     1261 cgttccggga ttttcaaact ctatactgta tactttgttg aaagatgtcc gtattttcaa
     1321 agaaacctca tcggacataa aggtatgtac atgtccgtct acaaccttaa aaccgtttat
     1381 catttcataa attacctctt ctcctatcaa aatataactc ttggtaatta accgtgtcat
     1441 acttatgtgc acaagtgtga aggaaaatat tgcagcagcg gcactaaaga atattataaa
     1501 tcattacctc cgccttatca attaaatgac gtgaagaaag aaattctttg cacacatttt
     1561 aaagtctgtt ataataaatt tgcatcgttt cactttgtgg aatgacacta taaatacaaa
     1621 aggaactgat taatatgaaa tacgtgttga ttttaggaga cggaatggca gattatccga
     1681 tccaacagct tgataacaaa actcctcttc aatatgccaa aaagaacaat attgattttc
     1741 ttgcacaaca ttcagaagtc ggcatggtca aaaccatacc cgacggaatt cctccgggaa
     1801 gcgacgcagc caatctctcc gttatgggat acaatcccag ggaatattat accgggcgtt
     1861 cgcccctcga agcagtcagc atgggtataa acctgtcgga taccgatgtt gccataaggt
     1921 gcaacttggt aacactgtcg gaggatgagg attacactca aaaaaccatg atcgactaca
     1981 gctctgatga aatctccact gaggaagcaa gggaacttat taatgatgta aacaggcact
     2041 taaaaagcca agacatcaat ttttacccgg gaatcagcta ccgtcattgc atggtgtgga
     2101 ataacggttt gacaggacta aaccttaccc ctccccatga catacttgaa aagaaaatta
     2161 cagattacct accgaagggg gaaatcacca aattattatt ggatatgatg gttaagagct
     2221 atgacatttt aaaagaccac cccgtaaaca aagccagaat aagtagaggg ctcaggccag
     2281 ccaattccat atggctctgg ggagaaggta aaaagcccct tttatcaaaa tactatgaca
     2341 ggtataaaat taacggctcg gttatttctg ccgttgacct cataaaggga ataggaatat
     2401 tggcaggact taaaaatgta gatgtggaag gtgccaccgg caacattcac accaatttta
     2461 tcggtaaagc acaggcagcc ttgaaggaat tggaatccgg tcaggacttt gtgtatctcc
     2521 attttgaagc accggatgaa tgcgggcaca ggtttgagat cgaaaacaag gtgaagtcca
     2581 ttgaaataat cgatgagcag gttgtgggaa caattttaaa gggtcttgaa aaatatgagg
     2641 attataaact tcttattctc ccggatcatc ctacaccgct gtctttaagg actcataccc
     2701 cggacccggt cccgtacctt ctgtatcaaa aatccaagcc caaaaagtct ggtgtcacgg
     2761 gatatgacga ggtacaggct gcaagtacga atattttctt caatgaggga tacaaactaa
     2821 tggaccattt tatattggat taggcttaac cgctgataac ttataaagta gtcctatatt
     2881 ggtgcataat tatcggtgat gcgaatttta tgatttaaga ggagaaaaat atgaaaaaac
     2941 attgtggaac aaggcttagc tgcccgatat ttttctcttc ttaaatttca taaaaacgta
     3001 cattttgcag cagcattcag ttcacaggat ttaaaaattg tattctataa aaactgctcc
     3061 ataaagtttt atacattctt tctcttcctc tgttaaattg agcctccatc ttgattcatc
     3121 atatgtatcg cctttgtcaa aaactacaat tctgtttatc attatttttg caatttcatt
     3181 gctaatgccg tttgttttaa gactgtccct gaactgttga ataatcccga cggtattaca
     3241 ggactcagtc tttcttgttt ccagggtttc catctctttt ttgatgaatg atatcttgct
     3301 ttccaactgt tgattcattc ttaagaaaag gctgtcggat attttttcgt caagcctatc
     3361 ctggtataaa atttcctgtt gcttttgctt tgcctccaac tgcttataaa gccgtttata
     3421 aactgtcctc cagtcttccg ttcccctgtc ctgtaaaatc tttcttaata tctgttccgt
     3481 tgcctcactg tcatttaaga gctcttcaag gtcgtttaaa acaatactgc ataattcccg
     3541 ttcccggatg tgatggctgg tacatgcgga ttttccgttt ttgccgtagc cgctgcaaat
     3601 ataactcatc ggccttccct gccttttcct tgcaaacata atacttccac aggccccgca
     3661 aaacatcata cccctgaata catgcaaaac gcctttgtgc ggtccggctt tgttcctttt
     3721 ttcatccctt atcctttgaa cctcataaaa ctcctctttt ggaataatcg gctcatgagt
     3781 gttctcggtt actacccagc agctttgggg aagacgccgt gtttttttac tcttaaagct
     3841 gattttttca cttaccccct gaacagtatc tcccatatat accctgttgg aaagaatcct
     3901 cattacccct accgcgttcc acagacccat tgacggagac tttaccccgt cttgatttaa
     3961 tttgacggct atgtaactat agccaaagcc ctccctgtaa agtctataaa ttctttttac
     4021 agtctcggcc tgttctttgt ttacaacaag cttgtttttt tcatgctgtg atttctcgta
     4081 gccatagggt gcattgccta tgtattcacc cttttgaatc ttaaaccgca ggttggttct
     4141 gatttttctc gaaatatcgc gaacataccg ctcgttgtac catgtgtcaa tacccacagt
     4201 gtcattgtct ctaatactgt catacctccc gtcaaaagta ataacccgaa ctcccttttc
     4261 ctccaaaaaa tcaaggaaca agagggtttt tgcgttattt cttccgagcc gggacaggtc
     4321 tttgattatt aaaagatcta tctcacccct tgacacatct cttttaagct cttcaattcc
     4381 ctttctctca aaaccggctc cggaaaccgt cgcgtgtata gcggaaaata tgctctttcg
//
LOCUS       DAOOJC010000182         4438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15387_length_4438_cov_4.004563, whole genome shotgun sequence.
ACCESSION   DAOOJC010000182 DAOOJC010000000
VERSION     DAOOJC010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15387_length_4438_cov_4.004563"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2193)
                     /locus_tag="PLA01_05960"
     CDS             complement(<1..2193)
                     /locus_tag="PLA01_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HPD00879.1"
                     /translation="MEHSVANFGTIIDNEEGTVKFVFSDDIELGFINLDGEFANIKAT
                     INETEKIGLSPIELVEEKFIDANLNPMIVNSNGGGVNVKEIEKTPTPEPTLSPTPTIE
                     PTQEPTPEPTTEPTETIDADYEKPVVTLEMSSTVVNVGSEVKIRATAVDNMKVKSVSA
                     QCDGVPVDLDENGMGTFKADTAGMFEINVVAVDAQGNEGYVKKDLFVKGKSDNKYPEV
                     SIEGPVQDSKVTSAVDIIGTVYDENLVKYVLEYSEKGKNQYIKFAEGNTSVNNGVLGR
                     LDPTVMRNGQYDIRITAYDSGGYTYSITATYMLEGEKKVGNFSIFFEDLAVPMAGLPI
                     SVTRTYDSRNKQKGDFGIGWTMALKDIKIDESYIPGKYWVQEEIGDKLSRRYNLYETK
                     EHIITVTFPSGRVDKFGIEVNPNFQKYEPLWETTVKFVAKEGRNSKLEALDVSNECIL
                     NGDGLVTSDIEYYNPDRYKLTTEDGTVYIINQNSGVEKITDANGNTVTFTKDGIVHSA
                     GKKVTFKRDNQGRITEITDPAGSSVKYEYDYYGDLVKVTDQEGNCVRFTYNSSHGLID
                     IIDPRGVKVARNEYDDNGRIIAHIDAEGNRIEYDRDLEGKQEIIKDRLGNVTVTSYDD
                     KGNVLLKTDPMGNTTSYTYDQRGNMLTETDPLGNTTTYTYDERGNITSITDPLGNKTE
                     YTYTEFGQRETRKDALGQIETKKYDSRGNITEKVDNKGNKTKYTFDNKG"
     gene            complement(2217..>4438)
                     /locus_tag="PLA01_05965"
     CDS             complement(2217..>4438)
                     /locus_tag="PLA01_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPD00880.1"
                     /translation="IEVPEETIPAVPQTPTPVPTATAKSTPTLQPTNNTSGSGTSGVI
                     IPGGKSDPVYRTYTSSEDFNEGQLNNVTGRIKDQLVLSDRDTDQSTKLENVYGKTQYP
                     LYIEVTQSVAKSVLMPSNDTVEVNVGLLGVGDPLVIERDPIDLVFAIDDSGSMEWGNT
                     DDVVENPNRLDHAKEASKNVVELLQSFDRAAVVEFAGSAWIQQDLTGDKAVLKQSIDN
                     TPVSPWDGTAIGLALSKSIEILENKSDAGRQKAILLLSDGGDNRWSSSEILQQAKKAK
                     EQGIRIYCVGLGSGVDQDLLKNISNTTEAGYSFSPTMEELEEMMFEAGKQIFDTAGRN
                     LVLETTIEDYVAADGTSPAPSKVIENSDGSKTYQWSYESLIIGGKKNISISFECNELP
                     DVSRVLLMKDTKLSYQDKDSRVVEVYTDDVLIPVSRYSDEGSWSTVFDSKRGETKWGS
                     IWWNGKIYNDGALKVKVSSSKDGQSFSNRVSVSNYGSFDIPNGRYVKIEVEFKRSSDG
                     FSPELFDITIAEKGYKIPSYKNAAPVIEEVLPVSGTVGKPSWLVSRVSDDCIPDGKMT
                     FSWEAVGKNAGKVGIKDAGSFIAKAVFSEPGKYSIELKAKDGGKVSQSVVEAVVTGTP
                     IPTMSPTPTPTKVPTPTMSPTPTPTKAPTPTPTLSPTPEPTPEPTPEPTPEPTPTPTH
                     SPTPSPTPTLSTLKVNVGTVEGEANEEIIIPVSFEGASKSGIKSCEFTLSFDPDVLK"
BASE COUNT         1068 a         1130 c          705 g         1535 t
ORIGIN      
        1 ccctttgttg tcaaatgtat atttggtttt atttccttta ttatcaacct tttctgttat
       61 atttcctctg gaatcatatt ttttcgtttc tatttgtccc aatgcgtctt ttctagtttc
      121 tctctgtcca aattcagtat aagtatattc cgtcttattt cccaaaggat ctgtaatact
      181 tgtgatattg cctctttcat cataggtata agttgtagta ttcccaagag gatcggtttc
      241 tgtaagcatg ttacctcttt ggtcataagt ataactcgtt gtatttccca taggatctgt
      301 ttttaagagg acattaccct tgtcatcata tgatgtaacc gttacattac ccaacctatc
      361 ctttattatc tcctgctttc cttccaagtc acggtcatac tcaatcctgt tgccctcagc
      421 atcaatatga gcaattatcc ttccgttatc atcgtactcg ttgcgggcaa ccttcacacc
      481 cctcgggtca ataatgtcaa ttaaaccatg gctcgaattg tatgtaaatc ttacacaatt
      541 accctcttga tcggtaacct taacaagatc gccataatag tcatattcat attttacact
      601 gctgcctgca ggatctgtta tctccgttat tcttccctgg ttgtcccttt taaaggtcac
      661 ttttttcccg gcagaatgta caataccgtc ctttgtaaat gtaacagtat tgccatttgc
      721 atctgtaatt ttttccactc cgctgttctg gtttattatg taaacagttc cgtcctcggt
      781 tgtaagcttg tatcggtcag ggttatagta ttcaatatct gaagtaacca gaccatcgcc
      841 attcaaaata cactcatttg aaacatcaag tgcttccagc tttgaatttc ttccttcctt
      901 agccacaaat tttacagtag tttcccataa cggttcatat ttctgaaagt ttggatttac
      961 ttcaatacca aatttatcaa ccctcccact gggaaatgta acagttatta tatgctcctt
     1021 tgtttcatat aagttatacc ttctgcttag tttatccccg atttcttctt gtacccagta
     1081 cttacccgga atataacttt catcgatctt aatatctttt aaagccattg tccagccaat
     1141 tccaaagtca cccttttgtt tatttcggct gtcataggtc cgggtaacgc ttatcggcaa
     1201 acctgccatg ggaaccgcaa gatcttcgaa gaatatagag aaatttccaa ccttcttctc
     1261 tccttcaagc atatatgtag cagtaatact atatgtatat cctcctgaat catatgcagt
     1321 aatccttatg tcatactggc cgtttcgcat cacagtggga tccagtctgc cgagtactcc
     1381 gttattgacc gaagtatttc cctcggcaaa tttaatatat tggtttttcc ctttttccga
     1441 atactccaaa acatatttta caaggttttc atcatataca gtaccgatga tatccactgc
     1501 agatgttacc tttgaatcct gaaccgggcc ttcaatcgat acctccggat atttattgtc
     1561 acttttcccc ttcacaaaca aatctttttt tacataacct tcattaccct gtgcatcaac
     1621 tgcaaccaca ttaatctcaa acattcctgc tgtatctgct ttaaaagtgc ccattccatt
     1681 ttcatccaaa tcaacaggta ctccatcaca ttgtgcactt acagacttta ctttcatgtt
     1741 gtctactgct gtagcacgta tttttacctc actgcccaca tttacaacgg ttgaactcat
     1801 ttccaatgtt acaacaggct tctcatagtc agcatcaata gtctctgtcg gttctgtcgt
     1861 tggctccggt gtaggttcct gtgtaggttc tatagttggt gtcggtgata acgtcggctc
     1921 cggtgttggg gttttctcta tttcttttac atttacaccg ccaccatttg agttaacaat
     1981 catcggattt aaattggcat caataaactt ttcttcaacc aattctatcg ggctcaatcc
     2041 tattttttca gtctcattta tcgttgcttt tatatttgca aactcaccgt ctaggtttat
     2101 aaagcccaat tctatatcat ccgagaatac aaattttaca gttccttctt cattgtcaat
     2161 aattgtaccg aaatttgcaa cagaatgttc gattatattc cctgcaataa cctcttctac
     2221 tttaaaacat ccggatcaaa agacaatgta aattcacagc tttttatccc gctttttgaa
     2281 gcaccttcaa aagatacggg aattattatc tcctcatttg cttctccctc aacagttcca
     2341 acattcactt tcagagtact cagtgtaggt gtcggtgatg gagtcggtga atgcgttggt
     2401 gtcggtgtcg gttccggtgt tggttccggc gttggttccg gcgttggttc cggcgtcggc
     2461 gacaatgtcg gtgttggcgt cggtgctttc gtcggtgttg gtgttggtga cattgtcggt
     2521 gtcggcactt tcgtcggtgt tggtgtcggt gacattgtcg gtatcggcgt tcccgtcaca
     2581 acagcctcaa caactgactg agaaaccttt cctccgtcct ttgcctttaa ctcaatagaa
     2641 tacttgcccg gctcactaaa cacagcctta gctataaagc ttccggcatc ctttatacct
     2701 actttccctg catttttacc gacagcttcc cagctaaagg tcattttacc gtcaggaata
     2761 caatcatcac ttaccctact taccaaccat gagggttttc ccactgttcc cgaaaccggc
     2821 aatacctctt caattactgg tgccgcattt ttatacgacg gaattttata tcccttttcg
     2881 gctattgtta tatcaaacaa ttccggactg aatccgtcgc ttgaacgctt gaattcaacc
     2941 tcaatcttaa cataccttcc gttgggtatg tcaaaactgc cgtaatttga cacactcacc
     3001 cgattactaa agctttgccc gtctttactg cttgaaacct taaccttcaa tgctccgtca
     3061 ttgtatattt ttccgttcca ccaaatgctt ccccacttcg tttcgccacg tttgctgtca
     3121 aatacagtac tccagcttcc ttcatcgcta tatctgctta ccggaatcaa tacatcatcc
     3181 gtataaacct caaccactct gctgtcttta tcttggtagc tgagctttgt atccttcatc
     3241 aataaaaccc ttgatacatc aggcaattca ttgcactcaa aagatattga tatattcttt
     3301 ttccctccga taatgaggct ctcataactc cattgatatg ttttgctccc gtcactattt
     3361 tcaattacct ttgaaggagc aggactggtt ccatctgctg caacataatc ctctattgtc
     3421 gtttccaaca ctaaatttct ccctgcagta tcaaaaatct gttttcccgc ttcaaacatc
     3481 atttcttcca gttcttccat ggtcgggctg aaagaatatc ctgcctctgt agtgtttgaa
     3541 atattcttca ataaatcctg gtccactccg cttccaagtc ctacacaata tattcttatg
     3601 ccttgctctt ttgccttttt cgcttgctgc aatatttcgg aagaactcca cctattgtct
     3661 ccgccatccg aaagcaataa aattgccttc tgccgtccgg catcactttt attttccaaa
     3721 atctctatcg acttgctcaa cgccaaaccg atagcagttc catcccatgg agataccgga
     3781 gtattgtcta tgctttgctt taaaacagct ttatcaccgg tcaaatcctg ctgtatccac
     3841 gcacttccgg caaactcaac taccgctgcc ctatcaaagg actgcaataa ctctacaaca
     3901 ttttttgatg cttcttttgc atggtccaga cgattgggat tttccaccac atcgtcagta
     3961 tttccccact ccatgcttcc cgagtcatct attgcaaata ccaggtctat aggatccctt
     4021 tcaattacca acggatcgcc aacacctaat aaccctacat tcacttctac cgtatcgttt
     4081 gacggcatta ataccgattt tgccacgctc tgagtaacct ctatgtataa aggatactga
     4141 gttttaccgt atacattctc caattttgtg ctttggtctg tatctctgtc cgaaagcacc
     4201 aactgatcct ttattcttcc ggtcacatta tttaactgtc cctcattgaa atcttccgag
     4261 cttgtatatg tacgataaac aggatctgac tttcctcccg gtattatcac ccctgacgta
     4321 ccgcttccac tcgtgttatt tgtcggctgc aaagtcggtg tggacttcgc cgtagccgtc
     4381 ggaaccggag tcggtgtctg cggtaccgcc ggtattgtct cttccggaac ttctatat
//
LOCUS       DAOOJC010000183         4423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15449_length_4423_cov_3.580128, whole genome shotgun sequence.
ACCESSION   DAOOJC010000183 DAOOJC010000000
VERSION     DAOOJC010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15449_length_4423_cov_3.580128"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /gene="metA"
                     /locus_tag="PLA01_05970"
     CDS             complement(<1..732)
                     /gene="metA"
                     /locus_tag="PLA01_05970"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HPD00881.1"
                     /translation="MPIKIPDSLPAKETLNNENIFVMDEQRALHQDIRPLRIAILNLM
                     PTKITTETQLLRLISNTPIQIDIELLHPKTHISKNTPLEHLTKFYKTFDEVKDEKFDG
                     LIITGAPVEQMEFEEVNYWNELKKIMEWSLTNVYSTFHICWGAQAALYYHYGIKKYPL
                     QEKMFGVFEHRICKPNTMLLRGFDERFYAPHSRHTEVRREDIEKVKEIDILSDSEQAG
                     VYIMKTDGGRQIFVTGHSEYDRFTLK"
     gene            complement(918..2225)
                     /locus_tag="PLA01_05975"
                     /pseudo
     CDS             complement(918..2225)
                     /locus_tag="PLA01_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
     gene            complement(2289..2528)
                     /locus_tag="PLA01_05980"
     CDS             complement(2289..2528)
                     /locus_tag="PLA01_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPD00882.1"
                     /translation="MNEKYISLNFLPLGKTAKVKFLTSDGIARRRMLDLGLISDTVVE
                     ALQKSPSGDPTAYHIRGAVIALRSEEASKIMVEIN"
     gene            2803..3291
                     /gene="mntR"
                     /locus_tag="PLA01_05985"
     CDS             2803..3291
                     /gene="mntR"
                     /locus_tag="PLA01_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HPD00883.1"
                     /translation="MKNNEFHTVRGYQLQKKESKTLTSAMEDYLEMIYRNVREGGYMR
                     INTLAELLNVRPSSATKMVQKLAMLGFLDYQRYGIISLTEKGKELGRFLLERHNIIER
                     FLKSIGIGENLLKETELIEHNVSANTLRSINQLNCFFKKHPEIIEKFEKYKMENNDDC
                     NL"
     gene            3857..>4423
                     /locus_tag="PLA01_05990"
     CDS             3857..>4423
                     /locus_tag="PLA01_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD00884.1"
                     /translation="MLVLEAGNIKKYYRDKLVIEFDDLKVYSGDRIGIVGQNGSGKTT
                     LLNILSRDIEPDQGFVKQYCDISYIRQFSDEGIKDADPKALKEFDLSHKAHQKAFSGG
                     EETRIKIVNAFSNESLLLFADEPTANLDFKGIDLLKQKLSEVETFLLVSHDRTLLDSL
                     CNKILEVCDSKIKIYTGNYSSYKEQKDIE"
BASE COUNT         1303 a          788 c          923 g         1409 t
ORIGIN      
        1 ttttaaggta aacctgtcat actcggaatg accggtcaca aaaatctgcc ttccgccatc
       61 agtcttcata atgtatacgc ctgcttgttc cgaatctgaa agtatatcaa tctcctttac
      121 cttctcgata tcttcgcgac gtacctccgt atgccttgaa tgaggagcat aaaagcgttc
      181 gtcaaaacct ctcagcaaca ttgtgttggg cttgcaaatg cgatgctcaa aaacgccaaa
      241 cattttttcc tgcaagggat atttctttat gccgtaatgg tagtacagtg ctgcctgtgc
      301 accccagcag atatggaaag tggaatacac gttggtaaga ctccactcca taattttttt
      361 aagctcattc cagtaattta cttcttcaaa ttccatttgc tcaactggtg caccggtaat
      421 tatcagaccg tcgaattttt catcctttac ttcatcaaag gtcttataaa actttgtcag
      481 gtgctctaag ggagtatttt tcgatatatg ggtctttgga tgcaaaagct caatatctat
      541 ctgaatcggc gtattactga tcaagcgaag aagctgtgtc tctgtagtga tttttgtcgg
      601 cattaaattc agtattgcga ttcttaaggg cctgatgtcc tggtgcagag ccctttgttc
      661 gtccattaca aatatattct cattgttaag cgtttctttc gccggtaaac tatcgggtat
      721 tttaattggc attaatagaa cctcccatac atttgttatc ctatatattt actcgtataa
      781 tttgaaatta tagcatattt gaaaattttt ttacacatca ttttcgatta tcaatgaaag
      841 tgctttgata tttatgtact tacaggctta attgttaagt gcagaaaaat gtgcaaaaaa
      901 tacattatcg gtttaaccat gcacatggtc agtgcctgct ttccgtgggc acaggctttt
      961 ttaaagaaat tgtcgagatt aaaggcgact cggggcaaat accccaagtc ttccagcagc
     1021 gtaaacaggg gaaagaatat agccatggga gggagcataa ccgatacaac ccatgccaca
     1081 gttctatata cacctgtaac caacaggctg tgaatccatt ccggggtatt cagccacagg
     1141 aagaagccgg ttagttgttc ctcgacccaa aagagaagtt tagaaagcat ttccgatgga
     1201 atattggccc ccacaatggt tagccagaaa ataccggcca gcagcataag cataatggga
     1261 atcccaaagg tccttgaagt aaggaacctg tctatttttc tgtcaaaggc gttatattgc
     1321 tttctgtcaa aggtgactgt tttcttgcat atttcctccg caaaggttat gagctttgag
     1381 acaattctgt ccctgaattg ttcggtttcg ataccgtttt gggacaggta tgattttgct
     1441 gcttcgaggg attctgtgag tttggcatca tcgagaagat tgaagccgag gtacttgttt
     1501 aacgattcga gcagggttga atccccgtct aacagcttta atgcgaccca tcggctgttt
     1561 attttatttt gcattttgtc aggtaatgaa tcctgaaccc tgtttattgc tttttcaatt
     1621 gtatcctcat atgcaatttc aattggtttt gtggcgataa ttccgttggc gacgttatag
     1681 acagcatcca ttaattcaga gagcccttta tttttcctgg ctgctgttgg aattacaggt
     1741 actccaagta tttctgacaa cttattataa tcaatattga tttttttcct ttctgcctca
     1801 tcgatgaggt ttacacaaac cacaacctta tcggttattt ccatggtttg cagaaccaga
     1861 ttaaggttcc gctcgagaca tgttgcatcg gttacgacaa cagtggtgtc aggctggcca
     1921 aagcatacaa aatctcttgc cacctgctcc tcaactgaat tggccataag tgaataggtg
     1981 cctggtatgt ctaccatgat aaaatttata tctttatgcc ggtattttcc ctgagcattg
     2041 gagacagttt ttcctggcca gtttcccgta tgctggttta gacctgttag actgttgaat
     2101 acagtactct tgcccacatt tgggtttcct gcaagtgcaa caaccttgtc atcgggtgcg
     2161 gttaattgaa ttttcagttc attcaccaga atacccattc cggtggattg gcttgtaaga
     2221 cccattcttg agatcctcct tattgtttgc ggatgattta taagcttttc aatatattat
     2281 aaattccatt agtttatttc caccatgatt ttgcttgcct cttcagaacg taatgctata
     2341 accgcaccac ggatgtgata agctgtcggg tcccctgagg gacttttttg caaggcttca
     2401 acaacggtat ccgaaatcag tccaaggtct agcattctcc tgcgggctat tccgtctgat
     2461 gtgaggaatt ttacttttgc tgtttttcct aaaggcaaaa agtttaaaga tatatatttt
     2521 tcattcatat agaaagcctc ctgttatatt atgtacaccg gttgtaatac ataagtgcaa
     2581 agcaaatact aagtttgcag aaaccttttc ttgtcttggc tgcgtttcaa tgaggcggga
     2641 gatatgttcg ccgccttaaa accccaatcg aaatacgcca cgttatttta acgtattgag
     2701 tggagagaaa ttttgtttct caagactaat atatgaaaaa taaaatgtta gtgttacgaa
     2761 tataattaac taattatata ttttatggag acagtgaaaa tgatgaagaa taatgaattt
     2821 catacagtaa ggggttatca attgcaaaag aaggaaagca aaacgcttac atcggctatg
     2881 gaggattatc ttgaaatgat ttatagaaac gttcgggagg gagggtatat gcgaataaac
     2941 accctcgcgg agcttctgaa tgtaagacct tcatctgcga cgaagatggt tcaaaagctt
     3001 gcaatgctcg gatttctgga ctatcaaagg tacggaatta ttagccttac ggaaaaagga
     3061 aaggagttgg ggagatttct tctagagagg cataatatta ttgagagatt tctaaaaagt
     3121 atcggaatcg gtgaaaacct gttaaaagag acggaattga ttgaacataa tgtaagtgcc
     3181 aacacactca ggagcattaa tcaattgaat tgttttttta aaaagcatcc tgaaattatt
     3241 gaaaagtttg aaaaatataa gatggaaaat aacgatgact gtaatttatg ataaatctat
     3301 aaggtgtata ttatttatgt gaagaaaaac atggaggaaa cattgcgaag taagttgcag
     3361 ggcctgttaa tactactgtt tgatgcaccc aattataggt taaatattgc tttgtgggat
     3421 attataatgt aaaatataga taaatctaac gaaagggata ggtatttaaa agatgaggat
     3481 tggttaattc agcagagaat tgaatagtac aggagttgta ttattagtgt agcggagcct
     3541 ttgagctttg ttttgtgctg gattgacctc cgtttagagg gatttaacaa agtgaaataa
     3601 taaatttaaa gtcctatatc cctgaagttt gtcagaaaac ttggaatata gccttattag
     3661 ctgataaaat tgatgtcact tggtttttaa ttattgatta ttaatatttt tagcttgtct
     3721 aggcttgtta tattcatatc acgaggttat tgtctttccg gcacattgtt atagcggttt
     3781 catcaactga aacgggcaaa gcaaaaaggt gctttgatgc tttgagctat attggatgtg
     3841 agaggagagt ttttttatgc ttgttttaga ggcaggaaac ataaaaaagt attatagaga
     3901 caagctcgtt attgagtttg acgatttaaa ggtttattcg ggagacagaa taggtattgt
     3961 tggtcaaaac ggttcaggga aaacgaccct gctgaacata ttgtctaggg atattgagcc
     4021 ggatcagggg tttgtaaagc agtattgcga catatcctac attcgccagt tttccgatga
     4081 gggaataaaa gatgcggatc caaaagccct caaggagttt gatttgagtc acaaagccca
     4141 tcagaaggca ttcagcggtg gtgaagagac aagaataaaa attgtaaatg ccttctcaaa
     4201 tgaaagcttg cttctgtttg ccgatgaacc taccgcaaac cttgacttta aaggtataga
     4261 cctgttgaag caaaagctgt cggaggtgga aacttttttg ctggtgagcc acgacagaac
     4321 gttgcttgac agcctttgca ataaaatcct tgaagtttgt gattcaaaga taaaaattta
     4381 caccggcaat tattcttctt ataaggaaca gaaggatata gaa
//
LOCUS       DAOOJC010000184         4418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15476_length_4418_cov_3.926656, whole genome shotgun sequence.
ACCESSION   DAOOJC010000184 DAOOJC010000000
VERSION     DAOOJC010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15476_length_4418_cov_3.926656"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLA01_05995"
     CDS             <1..188
                     /locus_tag="PLA01_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HPD00885.1"
                     /translation="SSNSFVATGAKISGSVIWDNVHVESGASVVNSVVMSGCRIDKNS
                     EKLNCVLTENYSCPIAE"
     gene            268..1443
                     /locus_tag="PLA01_06000"
     CDS             268..1443
                     /locus_tag="PLA01_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPD00886.1"
                     /translation="MVSDRIRNVAFLSTYPPRECGLATFTQDLVRELDKVEILNKPGV
                     IAVSNNDYTYSGRVLMELWQHDRESYKNVADAVNNSNIELLVIEHEYGIFGGEAGEYI
                     LDLVGNLRIPFVTTLHTVLPEPSEKQREVLRLLGEKSIKVVTMAKNTKPVLERVYDIA
                     PSKIEEIHHGVPYKTLESREELKKMHGLSGRSIVSTFGLISPGKGLEYGIEAIAKAAK
                     EHKDILYLILGQTHPCVKKESGEVYREKLVKMVDRLGIGEHVRFVDRYLSKDEIIKYL
                     QMSDIYMTPYLGKDQAVSGTLAYAVGYGKVIVSTPYSYAKEMLSEGRGLLAEFEDADS
                     LARHIIYILNNPKAKKEMEERTLSVGRTMMWEEVANHYARFFIDILEGMKPQGNMVL"
     gene            1440..2471
                     /locus_tag="PLA01_06005"
     CDS             1440..2471
                     /locus_tag="PLA01_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD00887.1"
                     /translation="MMEKLYSNITFKDDHIFRLTDDTGMLQHSKYSLPDPNHGYTADD
                     NARALIMALMLYEKYKEKKYLDLVYRYASFLLYAQNESGWFKNFMGYDRKWLEERGSE
                     DCFGRCMWALGYALSNDNTPKGVKQGLSYILKNALPNVAGLNWLRSKAYSIIGLAGLE
                     DKDAASLVFQIAQSLCRRYDEHRDGDWKWFENVVTYCNSVLPWSLIAAYRVTGEKRFW
                     DVAEESLEFLESITFKDGYFKPVGCDGWLSKGEKPAEFDEQPVEACEVVLTYMEAYEA
                     THNEQYLQKAKKCYAWYEGANSKGISLIDSETGGCYDGLTPKGVNLNMGAESLVSYII
                     SYMKISEVV"
     gene            2773..3288
                     /locus_tag="PLA01_06010"
     CDS             2773..3288
                     /locus_tag="PLA01_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00888.1"
                     /translation="MKAELFNGIVGYLEGISNIVSFYGVCYAHSYKFIRYDNVNDINE
                     CVEKFLNDSPIKEHRVKIDSVWELDDWKNDLFENCCDWFFRVFSMRNFEVSIEDEYGN
                     TMPAQKNKKSNRVFLGLLNRMEEFFGNDELKVYKLYLDPAWVDEFAWEQYFFQKGNEF
                     YVLSLFQEGLV"
     gene            3653..>4418
                     /locus_tag="PLA01_06015"
     CDS             3653..>4418
                     /locus_tag="PLA01_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00889.1"
                     /translation="MKIKNAKNTFIKRLLPIVSTAVLIVGMTAGCKLDTKKANADNSN
                     KQTESDTAGKQKDNQKDNQKEIMEQFQELLENASLIEIARFLDGNVSQVSSDNAVKMV
                     DEFEKRQKEFLPELDRKFVENEDIQRKMAEVFLQGFDISKIDSIEDEKVQELLKETKE
                     SGYKVETAEGMFFPVINYEVYKKYSDYVTDDLREYIDIMAVESNNTPAKDAALVISWE
                     EMLSRALKQEKFIAGYPDSIRIDDVKGLYKKYLTYTL"
BASE COUNT         1466 a          651 c         1050 g         1251 t
ORIGIN      
        1 taagttcaaa ttcttttgta gcaacgggtg caaagatctc aggaagtgta atctgggata
       61 atgttcatgt tgaaagcggt gcatcggttg taaactcagt ggtcatgtcc ggttgcagga
      121 ttgataaaaa cagtgagaag ttaaattgtg tattaactga aaattacagc tgccctatag
      181 ctgaataaat ttaaaatggg tgtttttcta acagaaagga acagttttaa aaaatgcata
      241 ataataaatg ggataggagg attcggtatg gtttcagata ggataaggaa tgttgcgttt
      301 ttaagcactt atcctccgag ggaatgtggc cttgctacat ttacacagga tctggtaaga
      361 gaacttgata aggttgagat cttaaacaaa cctggggtaa ttgctgtgag caataatgat
      421 tatacctaca gtggcagagt tttgatggaa ctttggcagc atgacagaga aagctataaa
      481 aacgtagcgg acgccgtaaa caattcgaat atagagcttc ttgttattga acatgagtat
      541 ggaatattcg gaggagaggc aggcgagtac atacttgacc ttgttggtaa ccttagaata
      601 ccctttgtta caacactcca taccgttctt cccgagccat ccgaaaagca aagggaagtc
      661 ttgaggctgc ttggagaaaa aagcataaag gttgttacca tggcaaaaaa cacaaagcct
      721 gttcttgaaa gagtctatga tatagcacct tcaaagattg aagagataca tcatggagtg
      781 ccatataaga ctctcgaatc ccgggaagag ctaaagaaaa tgcatggcct ttcgggccga
      841 agtattgtca gtacctttgg acttataagc ccgggaaaag gactggagta tggaatagaa
      901 gccatagcaa aagcagccaa ggagcataag gatattcttt atctgattct tgggcagaca
      961 cacccctgtg tgaaaaagga atcgggcgaa gtatacaggg aaaagctcgt aaaaatggtc
     1021 gacaggcttg gaatcggcga acatgtccgg tttgttgaca ggtacctttc caaggatgaa
     1081 atcataaaat atcttcaaat gtcagacatt tatatgacac cgtatctggg aaaagaccag
     1141 gcggtcagcg gaactcttgc ttatgctgtg ggatacggaa aggtcatagt atcaactcca
     1201 tacagctatg caaaagaaat gctttcggag ggaaggggac ttttggcaga gtttgaggat
     1261 gccgattcat tggcaaggca tatcatatat attctgaata atcctaaagc aaaaaaagaa
     1321 atggaagaga gaactttgag tgtcggcaga acaatgatgt gggaagaggt tgcaaatcat
     1381 tatgcccgat tctttataga tattcttgaa ggaatgaagc ctcagggcaa tatggtgtta
     1441 tgatggaaaa gctttattcc aatataactt ttaaagatga ccatattttc cggcttaccg
     1501 atgataccgg aatgctgcag cactcaaaat attcacttcc cgacccgaat catggatata
     1561 ctgcggatga caatgcaagg gcattgatta tggcactgat gctgtatgaa aaatacaagg
     1621 agaaaaagta tcttgacctt gtttaccgct atgcttcatt tttattgtat gcacaaaacg
     1681 aaagcggttg gtttaaaaac tttatgggat atgacagaaa atggcttgag gagagaggct
     1741 cggaggattg ctttggaaga tgcatgtggg cactgggata tgccctctcc aatgataata
     1801 ctcctaaagg agttaagcag ggcctttcgt acatattaaa aaatgcactg cctaatgtag
     1861 ccggactaaa ttggttgcgg tcaaaggcat attcaatcat aggtcttgcc ggtcttgaag
     1921 ataaggacgc agcgagtctt gtttttcaaa tcgcccaatc cctttgccgc cggtatgacg
     1981 agcacaggga cggggattgg aaatggtttg agaatgttgt tacatattgc aatagtgttc
     2041 ttccatggtc tcttatagcg gcttacaggg ttactggtga aaagcggttt tgggatgtgg
     2101 ccgaggaaag tctcgagttt cttgaaagta taacatttaa agacggatat tttaagccgg
     2161 tgggatgtga cggatggctg tcaaagggag aaaagcctgc cgaattcgat gagcaacccg
     2221 tagaggcatg tgaggtggtt ttaacataca tggaagccta tgaagcgaca cataatgagc
     2281 agtatttgca aaaggccaag aaatgctatg catggtatga gggtgcaaac tctaagggaa
     2341 tcagccttat tgatagtgaa accggagggt gctatgacgg actgactcca aaaggagtaa
     2401 acctgaatat gggagctgaa agccttgttt catatattat atcttatatg aaaatatctg
     2461 aagtcgtata attcaaagta tctggattta tattttttac ctcactcagt gaaagaggta
     2521 tattcatcga taataaaggg cggtatttcc ccgatatatt ccgtaacaag atgggtgaaa
     2581 gaggtatatt catcaataat aaagcagggc ttttcaagtg tatacaatcc atatggcttg
     2641 actgatgtat ctaagagtag taaactaaaa ttagctatta ttgacataca tatatataaa
     2701 ttataataac ataatggctt gttgtttggc tttttgtata attttcaaat aattttgcgg
     2761 gaggatttgt acatgaaagc tgaattattt aatgggatag ttggatactt agaggggata
     2821 tccaacattg tgagttttta cggagtgtgc tacgcacata gttacaaatt tatcaggtat
     2881 gataatgtaa atgacattaa cgagtgtgta gagaagtttt taaatgatag tccgattaaa
     2941 gaacacaggg ttaagattga cagcgtatgg gaattggatg actggaaaaa tgacctgttt
     3001 gaaaactgtt gtgactggtt tttcagagta ttcagcatga ggaacttcga ggtttccatt
     3061 gaggatgaat acggtaacac catgcctgct caaaagaaca aaaagagcaa ccgtgtattc
     3121 ttgggtttgt taaaccgaat ggaagagttc tttggaaatg atgaactaaa ggtgtataag
     3181 ctgtacttgg atcccgcctg ggttgatgag tttgcatggg agcagtattt tttccaaaag
     3241 ggaaacgagt tctatgtact tagtctcttt caggaagggt tagtctaaaa ggaatcacaa
     3301 aaaaatgagg ggtatatatt tattcggtta ttgttctcaa aaaggtttga tattaacact
     3361 tttgagaatt tttttatttg cagtacaaca atcaagaaaa aaatcaacat tttctaaagt
     3421 attattgcgg gtgtatatta ttcaagtcaa gaaaaatatg gaagaaacta gcatgactta
     3481 agttctcttc tatatttttc ttcaaactaa atgcacccta ttacttctta tgtaataaaa
     3541 gtataatgaa acattgtgga ggggttttgc gtctattaat aatatacgca ataattatat
     3601 gttgtcttta ttcaatgcgt aaatacatag tattttatag gagtggtttt gtgtgaagat
     3661 aaaaaatgcg aaaaacacct ttatcaagag attgctgccg attgtgtcta cagcggttct
     3721 tattgtgggt atgacggcgg gatgcaagct tgataccaaa aaagccaatg cagataactc
     3781 aaataaacaa actgaatcag atactgccgg aaagcagaag gacaatcaga aagacaatca
     3841 gaaggaaata atggagcaat tccaggaact gctggaaaat gcttcattga ttgaaatagc
     3901 tagatttttg gatggtaatg tctctcaggt ttcaagcgat aatgcagtaa agatggttga
     3961 tgaatttgaa aaaagacaga aggaattttt gcctgagctc gacagaaaat ttgtggagaa
     4021 tgaggatatc caaaggaaga tggccgaggt atttttgcag gggtttgata taagtaaaat
     4081 agacagcatt gaggatgaaa aggtacagga gctgttgaag gaaacaaagg aaagcggtta
     4141 taaagttgaa acggcggaag gaatgttttt ccctgttata aattatgagg tttataaaaa
     4201 gtacagtgat tatgtaactg acgacttaag ggaatatatt gatataatgg ctgtggaatc
     4261 aaataatact ccggctaagg atgcagccct tgttataagt tgggaagaaa tgctaagcag
     4321 ggcattgaaa caagaaaagt ttatagccgg gtatccggat tcaataagaa ttgatgatgt
     4381 aaaagggttg tataaaaaat atttgacata tacattgt
//
LOCUS       DAOOJC010000185         4394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15567_length_4394_cov_3.429131, whole genome shotgun sequence.
ACCESSION   DAOOJC010000185 DAOOJC010000000
VERSION     DAOOJC010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15567_length_4394_cov_3.429131"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="PLA01_06020"
     CDS             <1..1045
                     /locus_tag="PLA01_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HPD00890.1"
                     /translation="SGCTGWKPNQAKYAYSRDLASGWSSLYNLGNSTTYNSQPAYIVA
                     VQGTEETSYLYTGDRWAGAWGGKVNDSQYVWLPLEFVSDTKLELPYYDSITIDAAKGK
                     IYENFNNPPLYKIMNKNSGKLLDVQDGSMENAAQIVQWSDNGSLSQQWYIVDVGNGYK
                     RIVNAKSKKTLDVMNDSADNGGILIQYTSTGGYNQQWKFADAGDGYYKIVSRSSGKLV
                     DVYKWSTEDGATIQQWADAEGSNQHWKLIPVENTEPTPTPTVKPTPTATLKPTPTDAK
                     AIVLGDVNGDLSVDSTDITLMKRYMLRKISQFPDSNGKIAADVNGDSSVDSTDLTLVK
                     RYVLKKIDKFPAQ"
     assembly_gap    1117..1126
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1127..2191
                     /locus_tag="PLA01_06025"
     CDS             <1127..2191
                     /locus_tag="PLA01_06025"
                     /inference="COORDINATES: protein motif:HMM:NF021498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2220 family protein"
                     /protein_id="HPD00891.1"
                     /translation="VVRRFKMENCVYKIIENQNKKVIETADLLFVVERQFGGEVFELK
                     GGYRAFAAVIDSFCDKGLISPMKASGLNSRNPSLYKKYRVLINTRKQLSEEHMQELQS
                     LHPKLDKGFYFKNPDEYRKDRDYVLMLSDYLLDSNQNNTLKYRCTMNERSFEIFNNEK
                     FLESKGNVLLKRLRLSLEDLNCYKTHEAFFYILFEPKERLNILIIENKDTFYSALKYL
                     QRTSDREIYGVKLDMLIYGEGKKIVKSFSFINEVFVGTSIDRVYYFGDLDFEGIGIYN
                     SLASKYEKYLIVPHTALYKEMLYSAKSPPKLRTGQAEFFLDRFLMYFDEESRDRISQI
                     LYSGHYIPQEAVNFGRKTNC"
     gene            2169..3746
                     /locus_tag="PLA01_06030"
     CDS             2169..3746
                     /locus_tag="PLA01_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00892.1"
                     /translation="MDERQIVRGTVIDCLKDFRQRMINLAPFIHLSQKLSGFQKYTEV
                     DMVSVGFSVMLFILENMLIGREECSIEDIADFLQTLLKRCYDINMTQEESREMAFYIR
                     DSIAGSGGEVFEFKFRNLETNKDESILIKLIDTSYYEIKKSARYKLTDQGMELLFKTR
                     EIYSEFRINVTQMYLKQQIEKGVFSGALQTVNELNLQVRQLRERLESLLLNIRQNVLS
                     INFEELKKLFSRIKEQFEVERREFRNIKRILKEQRDNLEKISYEDLEEKELRNLEQIK
                     ILTERLNITSSEHDRLFNEKLDIVGEYLKTIEIRLRQGVSEFIDFEKSIFDLLISKDL
                     NPAPLKNMLSPVITNSASIKVFNPFKALEPQRIKAETQENERESLDIEAELEVKEKEE
                     REARERRDRKVMVYLEAIVLEAYHEGESFLSAAMKRLDEDTYNSAGFDFDFYSLIVML
                     HQNKLIDFKDLEDILEGMVRDTSSYNINIFYLFDRIFTKRPELKGLKSLILISTNKKL
                     KFKNGNEITDLVIKGVR"
     gene            3746..4394
                     /locus_tag="PLA01_06035"
                     /pseudo
     CDS             3746..4394
                     /locus_tag="PLA01_06035"
                     /inference="COORDINATES: protein motif:HMM:NF039975.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6063 family protein"
BASE COUNT         1490 a          709 c          979 g         1206 t
ORIGIN      
        1 ttccggctgt actggatgga aacccaatca ggcaaaatac gcatattcca gggatctggc
       61 cagcggctgg tcttcgcttt acaatctcgg caattccacc acatacaatt cacaaccggc
      121 gtatattgtt gcggttcagg gcacggagga aacatcctat ctctacacag gggacaggtg
      181 ggccggtgca tggggaggaa aggttaatga ctctcaatat gtatggctgc ccttggaatt
      241 tgtgtcggac acaaaactcg aactgcccta ttacgactca ataacgattg atgccgccaa
      301 aggtaagatt tatgagaatt ttaacaatcc cccattatat aagataatga ataaaaacag
      361 cggtaagtta cttgacgtac aggacggttc tatggaaaat gcagctcaaa ttgttcaatg
      421 gagtgataat ggctccttaa gtcagcagtg gtatattgtg gatgtgggaa acgggtacaa
      481 aaggattgtt aatgctaaaa gtaaaaaaac tctggatgta atgaatgatt ctgccgataa
      541 tggtggtatc cttatacaat acaccagcac aggtggctat aaccagcagt ggaagtttgc
      601 tgacgcaggg gatggctatt ataagattgt cagccgcagc agcggaaagt tggtggatgt
      661 ctataaatgg tcaactgagg atggagcaac tatccagcag tgggctgatg ccgaaggttc
      721 aaatcagcat tggaagctta ttccggtaga aaataccgaa cctactccta caccgacggt
      781 aaaacctacg cctactgcaa cattaaagcc cacacctaca gatgccaagg ctatagtttt
      841 aggagacgta aatggtgatt taagtgttga ttcaacagat ataaccctaa tgaaaagata
      901 tatgctgaga aaaatttcgc agtttcctga cagtaacgga aaaatagctg cagatgttaa
      961 tggggattca tcggttgatt cgacagacct tacacttgta aaaagatatg tgcttaaaaa
     1021 gatagacaag tttcccgctc aataaaacag ttggcagaag ggtgcatgtt gtaattttat
     1081 ccagaatact atataattat cgtgcaataa aaatttnnnn nnnnnngttg tcagaaggtt
     1141 caagatggag aattgcgttt ataaaatcat agaaaaccaa aacaagaagg ttattgaaac
     1201 tgctgattta ctttttgtgg tagagagaca attcggggga gaggtttttg agttaaaagg
     1261 aggatacaga gcctttgccg ctgttattga cagcttttgt gataaaggct tgatttcacc
     1321 gatgaaagca tcggggctca atagcagaaa cccttctttg tacaaaaagt acagagtttt
     1381 aataaatacc agaaaacagc tcagtgaaga gcatatgcaa gagcttcaat cccttcatcc
     1441 aaaactcgat aaaggttttt attttaaaaa tcccgatgag tacaggaagg acagggacta
     1501 tgtgctcatg ttatcagatt accttttaga cagcaaccaa aacaacacgc taaaatacag
     1561 gtgtacaatg aacgaaagat cctttgagat attcaacaat gaaaagttcc ttgaatcaaa
     1621 ggggaatgtc ttgctgaaaa ggctcagact ttctttggag gacttaaact gttataaaac
     1681 tcatgaggcc tttttttata tactgtttga accgaaagaa aggcttaata tattaattat
     1741 tgaaaacaaa gacacctttt attccgcatt aaaatacctt cagagaacgt ccgacaggga
     1801 aatatacggg gttaagttgg acatgcttat ttacggtgaa ggaaagaaaa ttgtaaaaag
     1861 cttcagcttt ataaatgagg tttttgtggg tacatccata gacagggtgt attatttcgg
     1921 tgatcttgat tttgaaggaa taggaatata caattcactg gcatcgaagt atgaaaagta
     1981 tttgatagtg ccgcatacag ccctttataa agaaatgctt tacagtgcca aatcacctcc
     2041 gaagctgaga acaggccagg cggagttctt tcttgaccgg tttctcatgt attttgatga
     2101 ggaaagcaga gacaggatat ctcaaatact gtatagcggt cactacattc cacaggaggc
     2161 ggtgaatttt ggacgaaaga caaattgtta gaggtacggt catagattgc ctgaaggact
     2221 ttcggcaaag aatgattaac ctggcaccct ttattcattt gtcacaaaag ctttcagggt
     2281 ttcaaaaata tactgaggta gacatggttt ccgtaggatt ttccgtaatg ctttttattt
     2341 tggagaatat gcttatagga cgcgaggagt gcagcattga ggatattgcg gattttttgc
     2401 agaccctttt aaaaagatgc tatgatatca atatgaccca ggaagaatcc agggaaatgg
     2461 ctttttatat aagggattca atagccggaa gcggtgggga ggtctttgaa ttcaagttta
     2521 gaaaccttga aaccaataaa gatgaaagca ttttgataaa gcttattgat acctcatatt
     2581 acgagataaa aaaatctgcg aggtacaagc tcaccgacca gggtatggag cttcttttca
     2641 agacgcggga gatctattcc gagttcagga taaatgtaac gcagatgtat ctaaagcaac
     2701 agattgaaaa gggggttttc tccggtgcct tacagacggt aaacgagctg aaccttcagg
     2761 tcaggcagct tagagagagg cttgagagcc ttcttttgaa tataaggcag aatgttttga
     2821 gtattaattt tgaggagcta aaaaagcttt tctctaggat aaaggagcag tttgaggttg
     2881 aaaggcgtga gtttcgcaac ataaagagga ttctcaaaga acaaagagat aacctggaaa
     2941 aaatcagcta tgaagatttg gaagaaaagg agttaaggaa ccttgaacaa ataaaaatac
     3001 tcaccgaaag acttaacata acatcttcag agcacgacag gctttttaac gagaagcttg
     3061 acatagtggg cgaatattta aaaaccatag aaataaggct tcgtcaaggt gtcagtgagt
     3121 ttattgattt tgaaaaaagt atttttgatt tgcttatctc gaaggattta aatccagcac
     3181 cattaaaaaa tatgttgtcc ccagttatta ccaatagtgc ctctattaag gttttcaatc
     3241 cctttaaggc cttagaaccc cagaggataa aggcagaaac ccaggaaaat gaacgggaaa
     3301 gtctagatat agaggcagaa ttggaagtaa aagaaaagga agagcgggaa gcacgggaaa
     3361 gacgggacag aaaggtaatg gtctatcttg aagcaatagt tcttgaggct taccacgaag
     3421 gagaatcctt tcttagtgcc gctatgaaaa gacttgatga ggatacatat aattcggcag
     3481 gttttgattt tgatttttac agcctgattg taatgctgca tcaaaacaag cttattgatt
     3541 ttaaggattt agaggatata ctcgaaggga tggtaaggga tacttcatcg tataatataa
     3601 atatttttta tcttttcgat cggatattta ctaaaagacc ggaacttaaa ggcttaaaat
     3661 ctctgatttt gatatcgacg aataaaaaat taaagtttaa gaacgggaat gaaataacgg
     3721 atcttgtgat taagggggta aggtgatgga aaatctatcg ccggattcgg ttagaaaggc
     3781 attttcactg tttattaaat taattgaaaa atcaaaaata acaaaaaagg ataatcccga
     3841 gttgttttca tcctatgacg atccggagat ttcatatatc atgagaattt ttgaggaaga
     3901 gtcaggaata atggtactgc attcttcgga taccctctat tttacgccgg gtatagataa
     3961 taaatttttg ggttttacaa acgaagaact gaggaacaaa atgggtttta caaataataa
     4021 ttctttcgat tataataaag ttttttaacg gtgaaaacta caataacaag gttcgtacca
     4081 tgctgaaggt ggaggaactg gaagcatata taacttctaa actgcaagcg gtggaagcca
     4141 tggaaaatca ggaagagatc gatgagagtt tggattttaa ttttaaaaac atttccaagc
     4201 tctggctgga tttgccgacc tatgatgaaa aaattaaaaa ctatggccag tcaaaaggca
     4261 ccaggataag ctttatatat agaactctca aatttttaca ggatcagggt ctggtgaatg
     4321 tcgacaatga cagtgagata tataccaccg ataagatgga taccattatt ataagttact
     4381 atcctgaaag cact
//
LOCUS       DAOOJC010000186         4371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15664_length_4371_cov_3.766219, whole genome shotgun sequence.
ACCESSION   DAOOJC010000186 DAOOJC010000000
VERSION     DAOOJC010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15664_length_4371_cov_3.766219"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PLA01_06040"
     CDS             <1..861
                     /locus_tag="PLA01_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPD00893.1"
                     /translation="SRSSVFMLKPLGSDSIKKIIKNALRDKERGLGNLEIEIDDKALD
                     YLAYICDGDARIALNSLEIAVLSSPIDKDGKLKIDVTLIEECVQRKAARYDKSGEGHY
                     DNISAFIKSMRGSNPDAAVFYLARALDAGEDPMFMARRIMICASEDVGMANPAALQVA
                     VAAAQAVHMVGMPEARIILSQAAVMVATSPKSNSCYVAIDKALNDVRNKRTGEVPMHL
                     RNAVTSGMKDLGYGKGYKYAHDYAGNYVEQEYLPEEMKGTVYYEPSGNGYELKIRDWL
                     LKKRNKAKES"
     gene            complement(1074..2102)
                     /gene="mtnA"
                     /locus_tag="PLA01_06045"
     CDS             complement(1074..2102)
                     /gene="mtnA"
                     /locus_tag="PLA01_06045"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPD00894.1"
                     /translation="MKPLEYCNGVLKLIDQTLLPTEHKTVELNNYVQVADAIKNMIVR
                     GAPAIGVTAAYGLAIAAKAINTDSKNEFFEQLKKVCDLIKSTRPTAVNLFWAVDRVYC
                     KAVSNRDKDIEEIKELIENEACLMEKEDVEANRSIGRYGNELIKEGSTILTHCNAGAL
                     ATCDYGTALGIIRAAHESGKNIRVFADETRPYLQGARLTAWELMQDNIPVTLICDNMA
                     GHFMKESLIDCVIVGADRIALNGDTANKIGTYSVAVLAKENNIPFYVAAPVSTIDFSI
                     ETGEQIPIEERNPVEITHIKGIRIAPEGVNVRNPAFDVTPNKYISAIVTDKGIIYPPY
                     YENIIKYR"
     gene            complement(2201..3007)
                     /locus_tag="PLA01_06050"
     CDS             complement(2201..3007)
                     /locus_tag="PLA01_06050"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HPD00895.1"
                     /translation="MNSKADIGVFGGSGFYSFLKDVEEIEIETPYGKPSDKIALATYE
                     GKRIAFLPRHGKNHQLPPHMIPYRANLFAMKKLGVKKILAPTASGSLQANIKPGDFVV
                     CDQFVDRTTGRKDTFYDGPVTKHISSAHPYCPKLRKIAIEAGQKLGITIHEKGTVVVI
                     QGPRFSTVAESRWFNKMGWDVINMTQYPEAYLARELGICYANISLITDYDAGLEGRDD
                     IAPVTEEAVLKVFEENNEKVKKMLFEIIKRIDLSDEHCTCNNVDLNGLDL"
     gene            3231..3398
                     /locus_tag="PLA01_06055"
     CDS             3231..3398
                     /locus_tag="PLA01_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00896.1"
                     /translation="MDNDIKDLEMFKYFLECYFHVDISYSELDDVIREFKQSELSKYS
                     AKLLEEAMLLK"
     gene            3474..4109
                     /locus_tag="PLA01_06060"
     CDS             3474..4109
                     /locus_tag="PLA01_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPD00897.1"
                     /translation="MEKREHYRELLLRQKNELENTINMMKAHDISEQNMEAADELSNY
                     DNHPAELGTAVFQAEMNNTLKVHEEHLLKEINDALTRIDKGDYGKCELCSKDIAEERL
                     EVLPYTRLCMDCESSKEITMDTLEWQRPVEELVWDAPLGRKYLNKQEDDEFEGMDQLN
                     DLVKFGSSDSPQDMGGYEDYEEFYTNEVDKQGVVDSMDNISNEEYKKQLPD"
BASE COUNT         1271 a          794 c          962 g         1344 t
ORIGIN      
        1 tcaaggtctt cggtttttat gctaaagccc cttggcagtg attcaataaa aaagattatt
       61 aaaaatgccc tccgggacaa ggagcggggg cttggaaatt tagagataga gattgatgat
      121 aaggctctgg actatctggc gtatatatgt gacggagacg cgagaattgc tttaaactct
      181 ttggaaattg ctgttttgag ttcacctatt gataaggacg gcaaacttaa aattgatgtg
      241 acgttgattg aggagtgtgt gcagaggaaa gccgcaaggt acgataaatc cggagaggga
      301 cattatgaca acataagtgc gtttataaaa tccatgaggg gaagtaatcc ggatgcagca
      361 gtgttctatc ttgcaagggc tttggatgcc ggggaggacc ctatgtttat ggcacggaga
      421 ataatgatat gtgcctcgga ggatgtggga atggcaaacc cggctgcgtt gcaggttgcg
      481 gttgcagcgg cacaggccgt tcatatggtg ggcatgcccg aggctcgaat aatattatct
      541 caggctgccg ttatggtggc aaccagtcca aagtcgaact cgtgctatgt ggcaatagat
      601 aaagctttaa atgatgtgag aaacaagcga accggtgaag tgccgatgca tttgagaaac
      661 gctgttacat caggaatgaa ggacctgggc tatggaaagg gctataaata tgcccatgat
      721 tatgcgggca actatgtcga gcaggaatac cttccggaag agatgaaggg aaccgtctat
      781 tatgagccct cgggaaacgg ttatgaattg aagataaggg attggctttt aaagaaaagg
      841 aataaagcta aggaaagcta aaaccgatga taaacgagtg atgaacgata aaacgggtaa
      901 tgaaaacaaa atttattgcg taaaattctg taaaaaagcc tcaagcttaa ttgattatta
      961 accttgaggc tttttagtgg gatttcacga tacccccgga gcttttgttc ggtatatcat
     1021 taacaattgc ctgagccatg catttttata gggcaaactg ttaattgttt ctcttaacgg
     1081 tactttataa tattttcata ataaggcgga taaatgattc ccttgtcggt aacaatggcc
     1141 gagatgtatt tatttggtgt aacatcgaat gcaggatttc ttacatttac accttccgga
     1201 gcaattctta tacctttaat gtgggtaatt tccacggggt ttctttcttc tatgggtatt
     1261 tgctctccgg tttcaatgga aaaatctata gttgatacgg gagctgcgac ataaaagggt
     1321 atgttgtttt cctttgctag aactgcaacg gaataggtac cgattttgtt tgccgtgtct
     1381 ccgtttagtg ctatcctgtc ggcacccact attacacaat caattaaact ctctttcata
     1441 aaatgccctg ccatattgtc acagatcagt gtaacaggaa tattatcctg catcaactcc
     1501 caggctgtaa gccttgctcc ctgcaaataa ggcctggtct catccgcaaa cacacggatg
     1561 ttttttcccg attcgtgggc agcacggata attccaaggg ctgtaccgta atcgcatgtg
     1621 gccagggcac cggcattaca atgggtaagt atggttgaac cttcctttat cagttcgttt
     1681 ccgtaccgtc cgatactcct gttggcctca acatcttctt tttccatcag gcacgcttcg
     1741 ttttcaatta actcttttat ttcttctata tccttgtccc tgttggatac agccttgcag
     1801 taaaccctgt caacggccca gaacaggttt actgcagttg gacgggtgct ctttatcaga
     1861 tcgcatactt ttttcagttg ttcaaagaac tcatttttag aatcagtatt tattgccttt
     1921 gcagctattg ccaacccata tgcggcagtt actcctattg caggagcacc tcttacaatc
     1981 atgtttttta tcgcatcggc gacttgaaca tagttattga gctccacagt cttatgctct
     2041 gtgggaagca aggtctggtc aatcagcttt aatactccgt tacaatattc caatggcttc
     2101 attaaaatct ccttttttaa aactttttat ctcaagtgga ggcatgctcc gttccactgg
     2161 cttttaaaca ctgttcaaac catggttaac tttacggaaa ctaaaggtct aatccgttta
     2221 agtcaacatt attgcatgta cagtgttcat cactcaagtc aattctcttg attatttcaa
     2281 agagcatttt cttaaccttt tcgttgtttt cttcaaacac tttgagtaca gcctcttcgg
     2341 taacgggggc aatatcgtca cgaccctcca aaccggcatc ataatccgta attaaagaaa
     2401 tgtttgcata gcatataccc agttctctgg ccagataggc ttcaggatac tgagtcatat
     2461 tgataacatc ccagcccatc ttgttaaacc atctgctttc tgccacagtt gaaaaccttg
     2521 gaccctgtat gactaccaca gttccttttt cgtggatggt aatacccagc ttttgacctg
     2581 cctcaatggc gatttttcta agcttggggc agtaaggatg ggctgagctt atgtgctttg
     2641 ttaccggacc gtcataaaaa gtatctttac gtcctgttgt tcgatcaaca aactggtcgc
     2701 aaacaacaaa gtcgccgggt tttatatttg cctgaagact gccggaggct gtgggggcca
     2761 gtatcttttt tactccgagt tttttcattg caaagaggtt agccctgtat ggtatcatgt
     2821 gaggcggcag ttgatgattt ttgccgtgac gggggagaaa agctattctt tttccctcat
     2881 atgttgccag tgcaatttta tcactgggtt ttccataggg tgtttcaatt tcgatttctt
     2941 cgacgtcttt tagaaacgaa taaaatcccg atccgccaaa aacgccgata tcagctttac
     3001 tgttcattgt aaacctccct tttgtaagtg attttcatta atatacttat tatattataa
     3061 aacctgggat tttcaataat tgaattatat atttactttt atatgattta aatatataat
     3121 gcctttttgt gagggaatat aatcaaatta aggcaataga aacacaaatt acaaaaagat
     3181 tcattaatct tgagaaagtg ctattttgtt attattaagg gagaactgcg atggataatg
     3241 atattaaaga tttagagatg tttaaatatt tccttgaatg ttattttcat gttgacataa
     3301 gttattctga attagacgat gtaatacggg aattcaaaca aagtgaatta tcaaagtatt
     3361 ctgctaaact gctggaagaa gcgatgttgt taaaataatt tttccatcag aaaccttcta
     3421 taaaaaacga ttatttgtgt aaatctaaag ttgaagaaag gaaggtaaac tgcatggaaa
     3481 aaagagaaca ttacagggaa cttctattaa gacagaaaaa tgaattggaa aatacaataa
     3541 atatgatgaa agcccatgat atttctgagc agaatatgga agctgcagat gagctgtcaa
     3601 actatgacaa ccatcctgcg gagttgggga cagcggtttt tcaggcagaa atgaataata
     3661 ccctcaaggt tcatgaggaa catttgctca aagaaataaa tgatgcatta accaggattg
     3721 ataagggaga ctacggaaaa tgcgagctgt gctcgaagga tatagcggag gaaaggcttg
     3781 aggttttgcc ttatacaaga ctgtgtatgg attgtgaaag cagtaaggag attacaatgg
     3841 ataccttgga atggcaaaga cctgtagaag agctggtgtg ggatgcaccc ttgggaagga
     3901 agtatctgaa taaacaggaa gatgatgagt ttgagggtat ggatcaactt aacgatttgg
     3961 taaagttcgg ttcatccgat tctccccagg atatgggagg gtatgaggac tatgaggagt
     4021 tttatacaaa tgaggttgat aaacagggcg tggtagacag tatggataac atttctaatg
     4081 aggagtataa aaagcagctg cccgattgaa tcaaatagag ttcaccgctg tatgcacgtt
     4141 tcagctttca tgcttttggt ttaagtttat tatttcaagg cttcagtgcg aggtgtaatc
     4201 ctcttctgaa gcctgatgtt cagtattatt tgagtgattt ttgtaggata gtcatattat
     4261 ttgtgcataa aataatttta ctgtagtttt tcaatacccc ccagacaaca agttatctag
     4321 ttgtttgtaa aatgctgtaa cccttgaaac caaagggttt tagcagtata t
//
LOCUS       DAOOJC010000187         4368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15677_length_4368_cov_3.555530, whole genome shotgun sequence.
ACCESSION   DAOOJC010000187 DAOOJC010000000
VERSION     DAOOJC010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15677_length_4368_cov_3.555530"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="PLA01_06065"
     CDS             <1..1149
                     /locus_tag="PLA01_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPD00898.1"
                     /translation="ESYDRLTANCSSEAPQIELDDEDDAAIYFSSGTTGFPKAILHNH
                     RSLMSACYTEQKHHGQTREDNFLCIPPLYHTGAKMHWFGSLLACSKAVLLRGIKPEWI
                     LRTVSEEKITIVWLLVPWAQDILDAIDRGDVKLEDYNLSQWRLMHIGAQPVPPSLIHR
                     WKKYFPNHQYDTNYGLSESIGPGCVHLGVENIHKVGAIGKPGFNWEAKIVDEKGCEVA
                     QGEVGELIVKGPGVMKCYYNDPQATAVVLKDGWLYTGDMAKMDEDGFIYLVDRKKDVI
                     ISGGENIYPVQVEDFLRSHDAIKDAAVIGLPDTRLGEIAAAIIELKPGFTCTEEDIIT
                     FCASMPRYKRPRKIIFDKVPRNPTGKIEKPRLREKYGAASLVAAQTQR"
     gene            1429..2094
                     /locus_tag="PLA01_06070"
     CDS             1429..2094
                     /locus_tag="PLA01_06070"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HPD00899.1"
                     /translation="MWALIAVPLVAILMVLVSCYLQRETVLMKETLKARIMAVFPAEP
                     LYIAFLAVSSIMGIITFGIRYYNHSNFIWAAKLTTLVILLAPISYIDFKTRKIPNFLL
                     LAGLSARVVFYIIEFFADNKAITGIITSDIKGLLLGGGIFLIGAVAAKNSIGMGDVKM
                     FAVIGLFCGYTKTMTVIMLSLFLCFITAVILLLMKRKKIKDSVPLAPFVFAGTFLAMI
                     LAG"
     gene            2425..2904
                     /locus_tag="PLA01_06075"
     CDS             2425..2904
                     /locus_tag="PLA01_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HPD00900.1"
                     /translation="MKIPTTLKHKPVIVSENYENVDGRYSYDTDAKGLSLGLAQWNDR
                     GKVDISAKVWRYTGEKWSRQSEELPLHRVLDLAILVCRTRQHFQDAYRFEKLYDTKNP
                     VIDRVGLQGDAMTVAVCTDNEKIDEDIKLFRQALSDDDELIGERLSTLSRILKEMGY"
     gene            2907..3257
                     /locus_tag="PLA01_06080"
     CDS             2907..3257
                     /locus_tag="PLA01_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPD00901.1"
                     /translation="MDRKKELKEMYKNMKPDMGVFIIRSNSDNRYFIQATPNLKGKIN
                     SVKFQLEGGVFVNRVLQREWNEKGESAFSIEILEKLEYDKDESKTDYSEELELLKIIW
                     EERLSQKGMEAYGK"
     gene            3302..4288
                     /gene="dcm"
                     /locus_tag="PLA01_06085"
     CDS             3302..4288
                     /gene="dcm"
                     /locus_tag="PLA01_06085"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HPD00902.1"
                     /translation="MNRLYNDLTFIDLFAGIGGFRIALESYGAKCVFSSEIDKYACKT
                     YEANFGEMPSGDITKIPAREIPRHDILCAGFPCQAFSISGKQLGFEDARGTLFFDIAR
                     IADFHKPKILFLENVKNLVYHNKGKTFSHMCDILDELGYKVFHKILNAGDFKVPQNRE
                     RVYIVCFRKDLGVDEFVFPKPLKEYIYLIDVLLPDSETHQYVIERDDIVITGKPDENC
                     RPGTIRIGTINKGGQGERIYSPYGHAVTLSAYGGGAAAKTGAYLVNGRVRKLAPRECC
                     LVQGFPRDYKIPVSDSQAYKLFGNSVAIPVLKAIFKNIISLDCMKGYNVKNR"
BASE COUNT         1357 a          721 c         1035 g         1255 t
ORIGIN      
        1 gaaagctatg accgtctgac tgcaaattgc tcttctgaag ctccgcaaat cgagcttgac
       61 gatgaggatg atgcggcaat atatttttca tcggggacca cgggttttcc aaaggcaatt
      121 ttgcacaacc atagaagctt aatgtctgcg tgttacaccg aacaaaaaca tcatggtcaa
      181 actcgcgaag ataatttttt gtgcattccg ccgctatacc ataccggtgc caaaatgcac
      241 tggtttggaa gcttgctggc atgcagcaag gctgtgttgc tccgcggaat taaaccggaa
      301 tggattctaa gaacggtttc cgaagaaaag attaccatcg tatggcttct ggtaccgtgg
      361 gcacaagata ttcttgatgc catcgacaga ggagatgtga agctggaaga ctacaatctt
      421 tctcagtgga gattgatgca tattggggca caaccggttc ctcccagttt gattcaccgt
      481 tggaaaaagt attttccaaa ccatcagtat gataccaact atgggctcag cgagtccatc
      541 ggtcctggat gtgtgcattt aggtgttgag aatatccata aagtcggtgc tatcggaaag
      601 ccgggattta actgggaagc taagattgtt gatgaaaagg gctgtgaagt tgcccaaggc
      661 gaagtgggtg aattgattgt caaggggccg ggcgtaatga aatgttacta taacgaccca
      721 caggccactg cggtagtctt aaaggatggt tggctatata ccggtgatat ggcaaaaatg
      781 gatgaagacg ggtttatata tttagtggat aggaaaaaag atgtgattat tagtggcggt
      841 gaaaacatat atccggtaca agttgaagat ttcctccgtt cccatgatgc gattaaggat
      901 gctgcagtaa taggattacc tgatacgcgg cttggtgaaa tagcagctgc aattatagag
      961 ctgaaaccgg gttttacttg cactgaagag gatataataa cgttctgtgc ttcgatgccg
     1021 cgttataaac gtccgcgaaa aattatattt gataaagttc cgcgtaatcc taccggaaag
     1081 attgaaaaac cgcgtttgag agaaaaatat ggtgcggcaa gtttggtcgc tgctcaaacc
     1141 caaaggtaat aacaggtatg ttatttcggg atgaaagtcc ctttgattta ataaccacag
     1201 agatttttat aatagatgca ctgtcaatca aacggtgtat ctttttttct gtataacgag
     1261 gtgataaatg tcccctgcaa tcgaaaaatt atctttgaac cgaggtgtta taaaatgtga
     1321 ttattgtcga tatctattaa gaggaaaaaa tgtagtattg ctggaccaaa attaaaaatc
     1381 gacagtgagt ttgccgggcc aaagcttgaa actggaggtg aatagtgaat gtgggcatta
     1441 attgcagttc ctttggtagc tatattaatg gttttggtga gctgttacct gcaaagggaa
     1501 acagtattaa tgaaagaaac attaaaagcc aggataatgg ctgtcttccc ggcagaacct
     1561 ttatacatag catttttggc ggtttcctct atcatgggga ttataacctt tggaatccgg
     1621 tattataacc attcaaattt catatgggct gcaaagctga caacattggt tattctgctt
     1681 gcacccatat cgtatattga ttttaaaacc aggaaaattc cgaatttttt gctgcttgca
     1741 ggtttgtctg ccagggtggt gttttatata atagagtttt ttgcagacaa taaagcaata
     1801 accggcataa taacttcaga tattaaaggt ttgttgcttg ggggagggat ttttctgata
     1861 ggggctgtgg ctgcgaagaa cagcatcggt atgggggatg taaaaatgtt tgccgtgatc
     1921 ggtctttttt gcggctacac caaaacaatg acagtaataa tgttgagctt gtttttatgc
     1981 tttattacag ctgttatttt gcttcttatg aaaagaaaaa aaataaagga ttcagtgccc
     2041 ttagctccgt ttgtatttgc cggaacgttt ttggcaatga ttttagccgg ttaggaagcc
     2101 tttccttgtt gaattaacag gttttcaact gcttttcaat attccttccg tattatttct
     2161 atttaatcat agttcactaa caggttattc aattgctttt caatattcct tccatattat
     2221 ttctatatta atcatagtcc gctcttatta tttagaaaaa agttagctga atatttggct
     2281 ttcttgttga catattaaaa aaacaagagt aaaatataat tagtaattta gtaatttagt
     2341 aaataagtaa gcggacatgg gagtaacgta gatttttttt aatacgtctc atgtcaaaag
     2401 agaggaaggg atatgaatat gaatatgaag ataccgacaa ccttaaagca taaacctgta
     2461 attgtgtctg agaactatga aaacgttgac ggaagatatt cttatgacac tgatgcaaag
     2521 ggactttcgt tggggctggc tcaatggaat gaccggggaa aggttgatat ttcagcaaag
     2581 gtatggagat acaccggcga aaaatggtcc agacagtctg aagagctgcc gctgcatcga
     2641 gtgctggatt tggcgattct tgtttgcaga accaggcaac actttcagga tgcctatagg
     2701 tttgaaaagc tgtatgacac taaaaatcct gttattgaca gggtgggttt gcagggagat
     2761 gccatgacag tggctgtgtg taccgataat gaaaaaatag atgaagacat taaattattc
     2821 agacaggccc taagtgatga tgatgagctt atcggtgaac gccttagcac cctgtcgaga
     2881 atattgaaag aaatggggta ctgattttgg atagaaaaaa ggaactaaaa gaaatgtaca
     2941 agaatatgaa accggatatg ggagtattta tcatccggtc aaattctgat aacaggtatt
     3001 tcattcaagc cactccaaac ttgaaaggaa agattaacag tgttaaattt caattggagg
     3061 gcggcgtttt tgtaaacaga gtgcttcaga gggagtggaa tgaaaaggga gaatccgctt
     3121 tttcaattga aatacttgaa aaacttgagt atgataaaga tgaatcaaaa accgattata
     3181 gcgaagaact ggaactttta aagataatct gggaagaaag gttatctcaa aaaggcatgg
     3241 aggcttatgg aaaataataa agtgtctgca gagctatgta aaaccttatg gggtgtagag
     3301 aatgaataga ctttataatg acttaacctt tatagactta ttcgccggaa taggaggctt
     3361 tagaatcgca ttggaatctt acggtgccaa atgtgtgttt tcttctgaaa tagacaagta
     3421 tgcatgcaag acctatgagg ctaattttgg agagatgccc agcggtgata ttacaaaaat
     3481 tcctgcccgg gaaatacccc gccacgacat actgtgtgca ggcttcccat gtcaagcctt
     3541 cagcatcagc ggcaagcagt tggggtttga agatgcaagg ggcactttgt tttttgatat
     3601 tgcccgtatt gcagattttc ataagccgaa aatactattt ttggagaacg taaaaaattt
     3661 ggtttaccac aataaaggca agaccttcag ccacatgtgc gacatattgg atgagttggg
     3721 atacaaggtt tttcacaaaa tactgaacgc aggggacttt aaagttcccc aaaacaggga
     3781 gagggtatat attgtatgtt tcagaaaaga cctgggagtc gatgagtttg ttttccccaa
     3841 acctttgaag gaatatatat accttattga tgtactgtta cccgacagtg aaacccatca
     3901 atatgtgata gaacgggatg atatcgttat taccggcaag ccggacgaaa attgcagacc
     3961 gggaaccata aggatcggca cgataaataa aggcgggcag ggagaaagga tatacagtcc
     4021 ctacggtcat gctgtaacct tgtccgctta tggcggaggt gcagctgcca aaaccggtgc
     4081 ttacttggta aacggacgtg tgcgtaagtt agcaccgaga gaatgctgtt tggtgcaggg
     4141 ctttccccgg gattataaga ttccagtatc cgatagtcag gcctataagc tgtttggaaa
     4201 cagtgttgcc atacctgtat taaaagccat ttttaaaaat attatcagct tggattgtat
     4261 gaaagggtat aatgtaaaaa acagataaac cggaaagaaa ctggtgaaag tcagtgaaat
     4321 agagattttg gatcgaaatt gaattaatgc agccgagttt aaacttgg
//
LOCUS       DAOOJC010000188         4364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15695_length_4364_cov_3.342307, whole genome shotgun sequence.
ACCESSION   DAOOJC010000188 DAOOJC010000000
VERSION     DAOOJC010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15695_length_4364_cov_3.342307"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PLA01_06090"
     CDS             complement(<1..482)
                     /locus_tag="PLA01_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00903.1"
                     /translation="MKYLENAFKFLGKNLLLSIPLIIMVVIPSVIQGSGNSLVLTDQI
                     NELQASYISGSIEYSDFLIMLFDLIRPLLILMAIASIVSLVMSFLIAPATYGMVNKAL
                     REGKADLTDFFPQMGKNILKFIIYNIANLVLWLIILIISGAIAALLVFLVIGLSNVSP
                     P"
     gene            complement(560..>4364)
                     /locus_tag="PLA01_06095"
     CDS             complement(560..>4364)
                     /locus_tag="PLA01_06095"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HPD00904.1"
                     /translation="LSNGSYSVMITDKGTGYSRNKHLDVTRWREDISQDNYGEFFYIR
                     NVKSNEAWSAAYAPFGRKPDEYKITFTSGKARFLRRDGNINTQTEMVVSAGDNAEIRR
                     ITLTNNGTEECVLEVTSYFELVLAPHGADIAHPAFSNLFIRTEFLPEHNCIIASRRPR
                     TENGKTLWAVSKMLVEGEAVGGLQYETDRMQFIGRGRDVSNPVALEPARPLSNTCGAV
                     LDPVVSFRQMVRVEPGKLVKVCFITAVTETREDAVEIAAKYSSAHVIKDEISMAITRS
                     RVEARYLNLVTEEIELFQDLISHILFIGPHHRKRQDCIKNNKKGQSALWSYGISGDIP
                     IVLVMLDKTEDIDIVQEVLKAHEYWKIKNFKIDLVILNEEENSYMNPLNGLLYDVLAG
                     SHAHDMINKPGGVFVLKGSNMPPEDIALICAVSRIILKGNAGDLREQMKYIKTSGLSE
                     LKSFKEEPVHYETRLNKDTDLVFFNGLGGFKQDGREYVIYLEYGHNTPLPWINVISNQ
                     KFGFIVTESGAGYTWFENSRENKLTPWSNDPVSDTPGEILYILDDHTAESWSVTPLPI
                     REGEPYRIRHGFGYTVFEHVSHGIGQEMTQFVPVEDSVKISILKLVNQTDKPRSLSMT
                     YYIRPVLGVSDQYTAAQINTEINSRGVVLIRNNYNDEFPGRVAFIDSSIKDRTVTCDR
                     KEFFGMGSIKDPEAIKRVSLSGTAGAGFDPCAAMSVNVSLEPGEEKELIFLLGAGKNA
                     EETEHLSKKYRKLEEAKKALEEVKKFWEEKLRAVQFHTQNTAMDILLNGWLMYQVISS
                     RLWTRSAFYQSGGAYGFRDQLQDSMAVTHVWPEVTRAQILLHSKHQFIEGDVQHWWHE
                     EKYKGTRTRFSDDLLWMPYVTEEYIRVTGDYAILEEETPFLEDEPLKEFEDEAYRIPR
                     VSGTVSTLYDHCIRAINRSLRFGEHGIPLIGSGDWNDGMNTVGNKGKGESVWLGWFLY
                     SILKGFAPLCERMGDTELAKSYLETADKIALAIEENAWDGSWYRRAYFDNGVPLGSIQ
                     NSECKIDSLAQTWAVISEGGNKDRIVEAMNALENYLVKRDEGLIQLLTPPFDQSDLEP
                     GYIKSYVPGVRENGGQYTHAAAWVVMAFAKMGDGDKALELFDLLNPINHSRTAIEYSR
                     YKVEPYVLAADVYSVPPHTGRGGWTWYTGSAGWMYRVGFEYILGFKKQGEFLVIDPCL
                     PKTWSGFDIKYRYKDAVYLIEVKNPDGVNRGVKKVMVDGAECTDKRISLVSDGKNHKA
                     EVYLG"
BASE COUNT         1154 a         1131 c          759 g         1320 t
ORIGIN      
        1 ggaggtgata cattacttaa tccaataact aaaaatacta gtaatgccgc aatagcccca
       61 gaaataataa gaataattaa ccacaaaact aaatttgcta tgttataaat tataaacttc
      121 aaaatatttt ttcccatttg aggaaaaaaa tcggttaaat cggctttacc ctctctaagt
      181 gctttattta ccataccata agttgccggt gctataagaa aactcatcac taatgataca
      241 attgaagcaa tcgccattaa tattaataac ggtctgatta aatcaaacag catgataaga
      301 aaatcagaat attctatact ccctgatata tagctcgctt gcagctcatt aatttgatcg
      361 gtcaacacca aactgtttcc actaccttga ataaccgacg gtataactac cataataatt
      421 aaaggtattg acagcaacag attttttccc aaaaatttga atgcattttc taaatacttc
      481 ataattatca ccccttaatc aatatactgc taaaaactta tttctattat agttttttct
      541 attgtttttg tctacaaaat catcccaaat aaacctctgc cttatgattc tttccgtcgc
      601 tgaccagcga aatcctttta tcagtgcatt ccgctccgtc aaccatgacc ttttttactc
      661 cacggtttac accatcggga tttttaactt caatcaaata cactgcatcc ttatacctgt
      721 atttaatatc aaacccggac caggtcttag gtaagcacgg atcaataaca agaaactctc
      781 cttgcttttt gaagcccaaa atatattcga atcctacccg atacatccac ccagccgatc
      841 ctgtgtacca ggtccagccg ccccttccag tgtgaggagg caccgagtat acatctgccg
      901 ccaatacata cggttcaacc ttataccttg aatattcaat agctgttctt gagtgattta
      961 tgggatttaa aaggtcaaaa agctccaggg ctttatcccc atcccccatt ttagcaaaag
     1021 ccataaccac ccaggcagct gcatgggtat attgtcctcc gttttcacgg acacccggaa
     1081 catagctttt gatatacccc ggttctaaat cactttgatc gaaaggaggc gtcaaaagct
     1141 gtatcaggcc ttcatcacgc ttaaccagat aattttccag ggcattcatc gcctcaacaa
     1201 ttctgtcctt gtttcctcct tcggatatta ctgcccatgt ctgtgccaaa gagtctatct
     1261 tacactcgct gttttgtatc gaaccgaggg gtactccgtt gtcaaagtat gccctcctgt
     1321 accagcttcc gtcccaggca ttttcttcta ttgccaaagc tattttatcc gccgtctcaa
     1381 gatagctttt cgccagttcc gtatccccca ttctttcaca aagcggagca aagcccttga
     1441 gtattgaata aaggaaccag ccaagccata cactttctcc cttccctttg tttccaacgg
     1501 tattcattcc gtcattccag tctccggagc ctataagcgg tattccgtgt tctccgaacc
     1561 tgagtgaacg gttaatagcc ctgatacagt ggtcatacag ggttgaaacg gtcccggaca
     1621 ccctcggtat acggtaagcc tcatcctcaa actcttttaa aggctcatcc tctaaaaatg
     1681 gtgtctcttc ctcaagaatt gcataatctc ctgtaactct tatatattct tccgttacat
     1741 agggcatcca cagaaggtca tcggaaaacc ttgtccttgt gcccttatac ttctcctcat
     1801 gccaccagtg ctgaacatca ccttctataa actggtgctt cgaatgaagc aatatttgag
     1861 ctctggtaac ctcaggccac acgtgggtaa cggccataga gtcctgaagc tggtctctga
     1921 acccataggc accaccggat tgatagaatg ccgaacgggt ccagagccgg ctggatatca
     1981 cctgatacat cagccagccg tttaaaagaa tatccattgc ggtattctgg gtatggaact
     2041 gtactgccct cagcttctcc tcccagaact tcttcacttc ctccagggcc ttctttgcct
     2101 cttctagctt tcggtatttc tttgacaaat gttccgtttc ttccgcattt ttccctgcac
     2161 ctaaaaggaa aatcaactcc ttttcttcac cgggttcaag actgacattt acgctcatgg
     2221 ccgcacaggg atcaaagcct gcccccgctg taccggaaag agaaacccgc tttattgctt
     2281 ccggatcttt aatactgccc attccgaaaa actcttttct gtcacaggtt acggttctgt
     2341 cctttattga agagtcaata aaggcaaccc ttcccgggaa ctcatcatta tagttgtttc
     2401 ttatcaaaac cactccccgg gaattgattt cggtattaat ttgagccgca gtatactggt
     2461 cactgacacc gagaaccgga cgaatatagt aagtcatgct caagcttctc ggtttgtcgg
     2521 tttggttcac aagctttaaa atgcttattt ttacactatc ctcaaccgga acgaactgtg
     2581 tcatttcctg tccgatgccg tggcttacat gctcaaatac cgtatagccg aaaccgtgtc
     2641 ttattctata aggttctccc tcccttatgg gaagcggagt cacagaccat gattctgctg
     2701 tatgatcgtc aagaatatat agtatttctc cgggagtatc acttacaggg tcattggacc
     2761 atggagtaag tttattttca cggctgttct caaaccatgt gtatccggct ccggattcgg
     2821 taacaataaa cccgaacttt tggttggaaa tcacatttat ccatggcagc ggcgtattgt
     2881 gaccgtattc gagataaatt acatactccc tgccgtcctg tttgaaacct cccagtccgt
     2941 tgaaaaatac cagatccgta tctttgttaa gcctggtctc atagtgcaca ggctcttctt
     3001 taaagctttt taactcagat aaacctgagg ttttaatata cttcatctgt tccctcaaat
     3061 ccccggcatt tccttttaat atgatccggg aaacagcaca aatcaaagca atgtcttcgg
     3121 gcggcatatt gcttccctta agaacaaata ctccccccgg cttgtttatc atgtcatggg
     3181 catggcttcc cgccaacaca tcataaagca agccgtttag cgggttcata taactgtttt
     3241 cttcttcatt cagaatcacc aggtcaatct tgaagttttt aattttccaa tactcatggg
     3301 ccttcaatac ctcctgcact atgtcaatat cctctgtttt gtccagcata acaagcacaa
     3361 tgggtatgtc tcccgatata ccgtatgacc acagagccga ctgccccttt ttgttgtttt
     3421 ttatacaatc ctgccttttc ctgtggtggg gaccgataaa cagtatgtgg gaaatcaggt
     3481 cctggaacag ctcaatttct tcagtaacaa ggttcaaata ccttgcctcc acacggctcc
     3541 tcgttatggc catgctgatc tcgtctttta ttacatgtgc agaagaatac ttcgcagcaa
     3601 tctcgacagc atcctcacga gtttctgtaa cagctgtaat aaagcacact tttactaatt
     3661 ttccgggctc tacccgaacc atctgtctga agctcacaac aggatccagc actgcaccgc
     3721 aggtattgga aagaggtctt gcaggctcta atgcaacggg atttgacaca tctcttcccc
     3781 tgcctataaa ctgcatccgg tctgtttcat actgcagtcc tcccactgct tccccctcta
     3841 ccaacatctt gcttactgcc caaagggttt taccgttctc agttcttggc cttctgctgg
     3901 caataatgca attgtgttca ggcaggaact ctgttcttat gaacaaattg ctgaaagcag
     3961 ggtgtgcaat atcggctccg tgaggggcca aaaccaattc aaaatagctg gtaacctcaa
     4021 gaacacactc ttccgttccg ttatttgtca aagtaatcct tcttatttcc gcattatccc
     4081 cggcactaac aaccatctcc gtctgtgtgt ttatattgcc gtcccttctc aaaaaccgtg
     4141 cttttcccga tgtaaaggta attttatact catcgggctt tctgccgaag ggggcataag
     4201 cagccgacca tgcttcgtta cttttaacat tccttatata gaaaaattct ccataattgt
     4261 cctggctgat atcttccctc catcgtgtca catccagatg cttgtttcta ctgtaacccg
     4321 ttcctttatc ggtaatcatt accgaatagc tgccgttgga aaga
//
LOCUS       DAOOJC010000189         4346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15788_length_4346_cov_3.257516, whole genome shotgun sequence.
ACCESSION   DAOOJC010000189 DAOOJC010000000
VERSION     DAOOJC010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15788_length_4346_cov_3.257516"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PLA01_06100"
     CDS             <1..232
                     /locus_tag="PLA01_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPD00905.1"
                     /translation="IGGGDIKLMASCGFVLGAPLGAMQSVISLVLAMIAGLCIRLSSE
                     SKKLNSITLPLAPFFCVGGIFSYLALLLSAIN"
     gene            254..1066
                     /locus_tag="PLA01_06105"
     CDS             254..1066
                     /locus_tag="PLA01_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HPD00906.1"
                     /translation="MNLSIFKNRTVIGLVCIFLSLVICFGLTPLFNSGLRAQTEIVRV
                     SSTVKNGEVLTADKLEMVKVGAYNLPDNVIKSKNSAVGKYVNADLQKGDYILASKLSE
                     KPLAEFEYLNDLDGTKVAMSITIKSFAAGLSGKLEAGDIVTLISANYDNLGKATIPPE
                     LQYVKVLAVTAETGDDKEYVPQKVSNGENEEKKLPATITLLVNTEQAKVLADLEQKGN
                     VHATLVYRGTNENANKFLAEQDKLFEKKEDTPNTVTNVKQNEHKEEGNVDAE"
     gene            1056..1808
                     /locus_tag="PLA01_06110"
     CDS             1056..1808
                     /locus_tag="PLA01_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD00907.1"
                     /translation="MPNKGKQILTVWGSPGSGKTVTALKLAKELADQKKNVLLLLCDD
                     ICPALPTIFKSKTPIDVSIGEVLTAPNITQEVILRNCVSFDKNPYISLLGYKAGENPF
                     SYAEYKKDRAVDLLVLLRHIADYVVIDCSSMLTESIISTVALEVADEVLRLGSCDLKS
                     VSYFRSVLPLLSEPKFNTDKHIKVLSNVRPSQDGEEYKNAFGGVSYTLPYVQAIDEQN
                     STLTLFESIPGKAAKIYDPVIKSIVKAVFGDE"
     assembly_gap    1845..1944
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1956..3308
                     /locus_tag="PLA01_06115"
     CDS             1956..3308
                     /locus_tag="PLA01_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF/VirB11 family protein"
                     /protein_id="HPD00908.1"
                     /translation="MSRNIGLFYNVAKNKKGFSEVLKEIQEYLSSKYATMIANNPAEQ
                     KQQITAYIAKYLTDYRLGVEGMDAEELVERLYTEMAEFSFLTQYLFATNIEEININSW
                     KDVKITYSDGRVLPSSEKFNSPEHAVDVVRRLLHKSGMILDNSQPGVVGHLSNKIRIT
                     VLGNPVTDADKGVAASIRIVNPQKLTRNDFIKNNTATAQMLDFLSACLRYGLSMCVTG
                     STGSGKTTLMSWLLSTVPDEKRIFTIENGCREFDLVKEDEKGNVINNVVHTVTRYSED
                     PKQNIDQEKLLEYALTCNPDVICVGEMKSAEAFAAQEAARTGHAVITTTHANSCTATY
                     HRMVTLCTQKYDINDKTLYNLVTEAFPLIMFVKKLEDNSRKVMEITECEICEDGTRKI
                     HTLFRYHITENTVVDGKVQIKGEYQKVGNISRSLQKRLLENGMPVSLLEKITEGADVA
                     "
     gene            3305..4234
                     /locus_tag="PLA01_06120"
     CDS             3305..4234
                     /locus_tag="PLA01_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00909.1"
                     /translation="MTLLILIAFAGLIAGFFLILNLSPFEFAESVVRLFQNRKTPIGK
                     RIEAIKNPKPVKGIRKTVRDAKEVLSLMGKEGKFGAICALSFFLMVIGLFASVLMDNF
                     LMIPVASIGLALVPFWYLIFTSHSYKKQMNAEMETALSIITTSYLRNESIITAVQENI
                     GYLNPPISNVFQSFLTQAKMINVNVKQALSDMKPKLNHYVFSEWVDAMIACQEDKTLK
                     TTLSPIISKLSDMRIVSAELDYLMYEPLKEFITMALLLVANIPLIYFLNRDWYSVLVN
                     TAFGKGILAICAFAILVSFAAVIRLTKPIEYKR"
BASE COUNT         1333 a          800 c          926 g         1187 t
ORIGIN      
        1 aattggcggt ggtgatataa agttgatggc ttcttgtggt tttgtgctgg gtgcacccct
       61 aggggctatg caaagcgtca tttctcttgt tcttgctatg atagcaggac tatgtataag
      121 actttcgagt gaaagtaaaa agctaaactc aatcacacta cctcttgcac cttttttctg
      181 tgtaggaggt attttttcat acttggcact tttgctgagt gcaatcaatt aaaaaacagg
      241 aggactattg agtatgaatt tgagtatttt taaaaaccga acagtcatcg gactggtatg
      301 tattttcctt tctcttgtca tctgctttgg gcttactcct ttgtttaaca gtggattgcg
      361 tgcacaaacg gaaattgtca gagtatcatc aacagtgaag aatggtgaag tgctaactgc
      421 tgacaagctg gaaatggtga aagtgggggc atataacctt ccggacaacg ttattaagag
      481 taaaaatagt gcagtcggga aatatgtaaa tgctgacttg caaaaaggtg actatatcct
      541 tgcttcaaaa ctatctgaaa agccccttgc cgagtttgaa tatttgaacg atttggacgg
      601 gacaaaggtt gctatgtcca ttaccatcaa gtcttttgct gccggcctgt caggcaaatt
      661 ggaagcaggt gatatcgtaa cccttatatc tgcaaattat gataacctgg gaaaagcaac
      721 cattccacct gaactccaat atgttaaagt cctggcagta accgcagaaa caggcgatga
      781 taaggaatat gtaccgcaaa aagtaagcaa cggtgaaaac gaagaaaaga aattaccggc
      841 gacgataact ttgcttgtca atacagaaca ggccaaggta cttgcagacc ttgagcagaa
      901 aggaaatgtt catgccacct tggtttaccg tggtactaac gaaaatgcca acaagttttt
      961 agctgaacag gacaaactct ttgagaaaaa ggaggatacc ccaaacacag ttacaaacgt
     1021 caaacagaac gagcacaaag aggagggtaa cgttgatgcc gaataaagga aaacaaattt
     1081 taacagtatg ggggagtcca ggaagtggca aaacggttac tgccttaaaa ctggcaaaag
     1141 agcttgcgga tcaaaagaaa aatgtgctgc tcctgctgtg tgatgatatc tgccctgcac
     1201 tgcctacaat ttttaaatcc aaaacgccga ttgatgtttc aatcggtgaa gtattaacag
     1261 ctcctaacat tacgcaggaa gtaattttaa gaaattgtgt ttcctttgat aaaaatccgt
     1321 atatcagttt acttgggtac aaggccggag aaaatccgtt ttcctatgct gagtacaaga
     1381 aagaccgggc agtggatctg ttggtccttc ttcgccatat tgctgattat gtggttatag
     1441 attgttccag tatgctgact gaaagtatta tttcaacagt agcattggaa gtggctgatg
     1501 aagtattaag gcttggaagc tgtgatttaa aaagcgtgtc ctatttcagg tccgtattgc
     1561 cactgcttag tgagcctaaa ttcaatacag acaaacatat taaagtactt tcaaatgtaa
     1621 ggccgtcgca ggatggcgaa gaatataaga atgcttttgg cggtgtttca tatactttac
     1681 cctatgtaca ggccattgat gaacaaaatt ctactctcac actttttgaa agtatacctg
     1741 ggaaagctgc aaaaatttat gacccggtca taaagtcaat tgtaaaggcg gtgtttggcg
     1801 atgagtagaa atatcggcct gttctataat gtggctaaaa acaannnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnggtgtt tggcgatgag tagaaatatc ggcctgttct
     1981 ataatgtggc taaaaacaaa aagggattct ctgaggttct aaaagaaata caggaatact
     2041 tatcaagtaa atatgccacc atgattgcaa ataacccggc agaacagaaa caacaaataa
     2101 cagcatatat cgcaaaatac cttacagatt acagattggg cgtagaaggc atggatgcag
     2161 aagaactcgt tgaacgcctg tacacagaga tggcagagtt ctctttccta acccaatatc
     2221 tttttgcaac caatattgag gagattaata tcaacagttg gaaagatgtc aagataactt
     2281 attcagacgg aagggttctc ccttcctctg aaaagtttaa cagtccggaa cacgccgttg
     2341 acgtggtccg cagattactt cacaaatccg gtatgatact tgataattca cagcccggtg
     2401 tggtggggca tctttccaac aaaatccgta ttactgtttt aggaaaccct gttactgatg
     2461 ctgataaggg cgtggctgct tcaatccgta ttgttaatcc tcagaagctg acgagaaatg
     2521 attttataaa aaataatacg gctactgcac agatgttgga ttttttaagc gcctgtttga
     2581 gatatgggct gtcaatgtgc gtaaccggca gtaccggttc aggcaaaacc actctgatga
     2641 gctggctttt atccaccgtt cccgatgaaa aacgtatctt taccattgag aatggctgcc
     2701 gtgaatttga ccttgtaaaa gaggatgaaa agggcaacgt catcaacaat gtggtgcata
     2761 ccgtcacccg atattcggaa gatcccaagc agaatattga ccaggaaaaa ctcctggaat
     2821 atgcccttac ttgcaaccct gatgttatct gtgtcggcga gatgaaatca gcagaagctt
     2881 ttgcagcaca ggaagcagct cgaaccggcc atgccgttat cactaccacc catgccaaca
     2941 gttgtaccgc cacataccat cgtatggtga cactgtgtac ccagaaatat gatatcaatg
     3001 ataaaacctt atataacctt gtgacagaag cctttccgct gatcatgttt gttaagaagt
     3061 tagaggataa cagccgtaaa gttatggaaa taacagagtg tgagatttgc gaagacggga
     3121 cacgcaaaat tcacactctt tttcgttacc atatcactga gaatacggtt gtagacggca
     3181 aggtgcagat taagggtgaa tatcagaagg tcggcaatat atcacgtagc ctgcaaaagc
     3241 ggcttcttga aaacggtatg cctgtgagtc tgcttgaaaa aatcacggaa ggagctgatg
     3301 tagcatgaca ttgcttattt taatcgcctt tgcaggtctg attgcaggat tctttctgat
     3361 tttaaattta tcgccttttg agtttgccga aagtgtagta aggctatttc agaaccgcaa
     3421 aacaccgatt ggaaagagaa ttgaagcaat taaaaatcca aagcctgtta aaggaatcag
     3481 aaaaaccgta agagatgcca aagaggtttt atccctcatg ggcaaggaag gaaaatttgg
     3541 agcaatatgt gcgttatcct tttttctcat ggtaattggg ttatttgctt ctgtcctcat
     3601 ggataacttt ttaatgattc ccgttgcttc aattgggttg gcacttgtgc ctttctggta
     3661 tttgattttt acttctcatt cctacaaaaa gcaaatgaat gcagaaatgg aaacagctct
     3721 gtcaatcatc accacaagct atttgagaaa tgagagtatc attactgctg ttcaggagaa
     3781 tatcggctat ctgaatccac ccatctccaa tgtctttcaa tcattcctga cacaggcgaa
     3841 aatgattaat gttaatgtga agcaggctct ttccgatatg aaaccaaagc tgaaccatta
     3901 tgtttttagc gagtgggtag atgccatgat tgcctgccag gaggacaaaa cattaaaaac
     3961 tacattgtcg ccaatcatat caaagctttc tgatatgcgg attgtttcag cagaactgga
     4021 ttatctgatg tatgaaccat tgaaagaatt tatcacaatg gctctgcttt tagtggcaaa
     4081 tatcccgctt atttatttcc taaataggga ttggtattca gtgcttgtga atacggcatt
     4141 tggtaaaggg atactggcaa tatgtgcctt tgcaatactc gtttcctttg ctgcagtaat
     4201 caggcttacc aagccaattg agtataaaag gtagtgtcaa atcaaagcca cagatcttta
     4261 atgcaaatat aagcaagaaa ggagttttcg ccatgttaca agataaaaca attaaacaga
     4321 aagattatga aagagcttcc caactt
//
LOCUS       DAOOJC010000190         4344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15798_length_4344_cov_3.848916, whole genome shotgun sequence.
ACCESSION   DAOOJC010000190 DAOOJC010000000
VERSION     DAOOJC010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15798_length_4344_cov_3.848916"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PLA01_06125"
     CDS             complement(<1..1005)
                     /locus_tag="PLA01_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPD00910.1"
                     /translation="MLFSRDIGIDLGTANTLVHVKGKGIIVREPSVVAINKKNGEILA
                     VGDSAKDMIGRTPGNIVAIRPMKDGVIADFEVTQSMLKYFIKRAMSKGVFGKPRVVIC
                     VPSGVTEVEKRAVEEATINAGAKEAYLIEEPMAAAIGANLPVEEPSGSMVVDIGGGTS
                     EVAVISLGGIVASKSLRIAGDELDDSIVHYIKKEYNLMIGERTAEEIKVTIGAAYKMV
                     KEENMEIRGRDLITGLPKNITITASEVMEALKDPIYAIVDSIKITLEKTPPELAADIM
                     DRGIMLTGGGALLKGLDRLIRDETGMPVNIAENPLDCVVMGSGKVLEEIETLKKVLIS
                     P"
     gene            complement(1131..1745)
                     /locus_tag="PLA01_06130"
     CDS             complement(1131..1745)
                     /locus_tag="PLA01_06130"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPD00911.1"
                     /translation="MYYEVITIQIVLASASPRRSELLKQIGLDFIIVPSKIDEGNNDN
                     LSPAELVQYLAYQKAMDVAEQMAQTGHNNRYLVIGADTVVVKDGIIGKPADRHEAFCI
                     LSRLRGGWHEVFTGIALIDTRDFKYIKGFETTRVKMKEMSDDTIFAYIDSQEPMDKAG
                     AYGVQGKGAVLVERVEGCYFNVVGLPLPRLAELLKEFGVCVFKH"
     gene            complement(1963..2901)
                     /locus_tag="PLA01_06135"
     CDS             complement(1963..2901)
                     /locus_tag="PLA01_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00912.1"
                     /translation="MKKNTGMGETAQNEGTSTEIQYNQEKDFYRGIGGELRMKAEGLF
                     KKAKEKGISIEDLSIDVLKEKGVEFPGVGNVELPSYLVKIRGKDISSGQVIVDGKQID
                     YYNRYQKYIAQKIEGKNAIKDERGSKVIDNRKPVIKNNPEFALTEWEMFEIAKELIDD
                     KEFGLEKTITGACDRIIRKLMGENDWLYPEEARLLDEEFSTVQSRLVSEQEKKQASSN
                     KTPVKKATQRQINYFKQRLKSMDMDPGDPAVLNKILMKVGVEQKDIDDLSVGEMSKII
                     DSLNSVVPEIKDDMQKIDVSAIDETGTGGDKGIVKQ"
     gene            3288..3740
                     /locus_tag="PLA01_06140"
     CDS             3288..3740
                     /locus_tag="PLA01_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPD00913.1"
                     /translation="MLKLALKYALFAGISTLSNLTTQHLVQMLLSSLGMEKDFVLDIF
                     GVFSIKVILLIAIFFGTLVALVIKYVLDKRFIFNFETKSNSENTGKFLLYSFMGIFTT
                     LIYWVFELSFDFLFDYESAKYIGALIGLSMGYVIKYQLDKRFVFISKR"
     gene            3846..>4344
                     /locus_tag="PLA01_06145"
     CDS             3846..>4344
                     /locus_tag="PLA01_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HPD00914.1"
                     /translation="MKIEKINDNMLKVTISIDDLEERNIDIDTWSYNSPATQKLFGDM
                     MEQAEIQFGFCVSGSEICVEAAPDTDEGFVIIITKLDEDEEFESIEKYIKNRFKKADL
                     KSKKKSSGICSTILIYCFNNFDDLCLLSQRLIRIYTGESTLYKLKGTYYLLLTKNSWS
                     VDNTFP"
BASE COUNT         1208 a          968 c          665 g         1503 t
ORIGIN      
        1 aggagaaatt agcaccttct tcaacgtttc tatttcttca agaacctttc cggaccccat
       61 aaccacacaa tccaacggat tctcagcaat attcacaggc atgcctgtct catccctgat
      121 aagcctgtcc agacctttta acagtgctcc tccaccggta agcattattc ctctgtccat
      181 aatatcagct gcaagttccg gcggtgtctt ttcaagagtt atttttatcg aatctactat
      241 agcataaatt gggtctttta atgcttccat aacttccgaa gctgttatgg ttatattctt
      301 gggaagtcct gttataaggt ctcttcctct tatctccatg ttctcttcct tgaccatttt
      361 atatgctgcc cctatggtaa cctttatttc ttcggcagtc ctttctccta tcattaaatt
      421 atattctttt ttaatgtagt gtacaataga atcatcaagt tcatccccgg caatcctcaa
      481 agatttactg gcaactatgc ccccaaggga tataactgca acctcactgg tccccccgcc
      541 tatatcgaca accatgcttc ccgatggctc ctccaccgga agattcgctc ctatcgcagc
      601 tgccataggc tcttctataa ggtatgcctc cttggcacct gcattaatgg ttgcttcctc
      661 cacagcccgt ttttcgactt cagtaacacc cgacggaacg caaataacaa ccctgggctt
      721 cccaaataca ccttttgaca tggctctttt gataaaatac ttgagcatac tttgagttac
      781 ttcaaagtct gcaataactc catccttcat tggccttatt gcaacaatat tcccaggagt
      841 ccttccaatc atatccttcg cagaatcgcc aactgcaagt atctcaccgt tttttttgtt
      901 tattgcaacc actgaaggtt ctctgactat aatgcccttt ccctttacat gaaccaatgt
      961 attagctgtt cccaaatcaa ttcctatgtc ccttgaaaat aacatttatc ttctcctccc
     1021 gaataatcta aattccagga ttccacctta tgaaaggcag aattcctgcc tccaactcca
     1081 gcggggtcga atactctccg aagcccctat gttcagctta tccaactagt ctaatgctta
     1141 aaaacacaaa ctccaaattc cttaagcaat tcagccagcc gtggcagggg taaacctaca
     1201 acattgaaat agcagccttc aactctctct accagcactg ctcccttacc ctgtacacca
     1261 taggcccctg ctttatccat aggctcttgc gaatctatat aggcaaaaat agtatcgtca
     1321 gacatttctt tcatcttgac ccgtgttgtc tcaaaaccct tgatgtattt aaaatcccgt
     1381 gtatcaataa gagctatacc tgtaaatact tcatgccagc ctcctctcag gcggcttaat
     1441 atgcaaaagg cttcgtgcct gtcagcaggc tttcctatta ttccgtcctt tactactacc
     1501 gtatccgcac caataacaag atacctgtta ttgtgcccgg tttgagccat ttgttcagca
     1561 acatccattg ctttttgata tgcaagatat tgtacaagct cagccggact taaattatca
     1621 ttattgcctt catctatttt cgatggaaca attataaaat caagacctat ttgttttaaa
     1681 agctcagatc ttcttggtga tgcggatgcc aaaacaatct gtatggtaat cacctcgtag
     1741 tacatactaa aacagtctat atgagatatg attgaagcat taaacttcaa acatacacta
     1801 ttataatcat ataaatcgct ttatttcaac aaaatatgaa tttttttgat tactttttcg
     1861 ttacaattat actacataat ataaacaagt taaatagaaa tttagtccaa cttgaatata
     1921 ttaatattaa ttattttaat taccagtcaa catataataa ttctactgtt tcacgattcc
     1981 tttatcccca ccggttcctg tttcatctat tgctgacaca tcaattttct gcatgtcatc
     2041 cttgatttca ggtactacgc tgtttaagct gtcaataatc ttactcattt ctcctacgct
     2101 caggtcatcg atatcttttt gttcaactcc cactttcatg agaatcttgt ttaacacggc
     2161 cggatcgccg ggatccatat ccatactctt cagtctttgt ttaaagtaat tgatctgcct
     2221 ttgtgttgcc ttctttaccg gtgtcttatt cgaacttgcc tgttttttct cctgctcgct
     2281 gaccagacgg ctttggacag tactaaactc ctcgtccaaa agtctcgcct cttcagggta
     2341 aagccagtcg ttttctccca taagctttct aattattctg tcacatgcac cggtaatagt
     2401 tttttcaagg ccgaattcct tatcatcaat aagttcttta gctatttcaa acatctccca
     2461 ttctgtaagt gcaaattcgg ggttattctt tatcacaggt ttcctgttat ctataacctt
     2521 acttcccctt tcatccttaa ttgcgttctt accctcaatc ttttgtgcaa tatacttttg
     2581 gtatctgtta taatagtcta tctgttttcc gtcaacaata acctgtccgc tggaaatatc
     2641 ctttcctctt attttaacca ggtatgaagg caattcaaca tttccgacac cgggaaattc
     2701 tacccctttt tccttcaata catcaatcga caagtcctca atggaaatcc ccttctcttt
     2761 tgctttctta aaaagtccct ccgccttcat tctcaactcc ccaccgatgc ctctatagaa
     2821 atctttttcc tgattatatt gaatttcggt tgacgtaccc tcattttgag ctgtttcgcc
     2881 catacctgta ttctttttca tgtttccatc tccttacagc aagaacgttg ttaatcggtc
     2941 ttattctatt gactattgaa tttaatagct atttatattt tgattattat tatttgatta
     3001 aatgcaatta tcaaaacagc tttttttttc attaaaaaga aagatttcta cagttttcat
     3061 tttttatgta tcggtattgt ctgtgtcgta cgggggcacc ttaatgcata gaaaaatgtt
     3121 tcagaaactt acgtttcgca tgtccagagc ctgtaaataa tgtttcttct acatttttca
     3181 ttacagtaat aatttatttc acacatccga tatataattt gacaaatgtg ccggcacact
     3241 ctacaatcat attattattt attatcaatg gatgggcggg atatcacttg ttgaaattgg
     3301 cactgaaata tgctctcttt gcaggcatat ccacattgag taatttaact actcagcact
     3361 tggttcaaat gcttctttcg tctcttggaa tggagaaaga ttttgttctt gacatatttg
     3421 gagttttcag cataaaggtc atactcctta ttgctatttt cttcggaaca ttagtagctt
     3481 tggtaattaa gtatgtattg gacaaaaggt ttatattcaa tttcgagaca aaaagcaatt
     3541 ccgaaaacac aggaaaattt ttgctttatt cgtttatggg aatatttaca acacttatat
     3601 actgggtttt tgagctctcc tttgactttc tctttgacta tgaatccgca aagtatatcg
     3661 gtgcattgat tggactttcg atgggttatg tcataaagta ccagctggat aagcggtttg
     3721 tttttatcag caaacgatga actatattta accgtataga aatattttat acttctcttt
     3781 tttgaataat agaagtataa tatagataag gcatacaatt atgtaaagaa ttcatggtga
     3841 tacttatgaa aattgagaag attaatgata atatgcttaa agttacaatc tcaatagatg
     3901 atctcgaaga aagaaatatt gatatagata catggagcta taattccccc gcaacgcaga
     3961 agttgtttgg ggatatgatg gaacaggccg aaatacagtt cggtttctgc gtttcaggtt
     4021 ctgaaatttg tgttgaggca gcaccggaca cggatgaagg atttgtcatt ataattacca
     4081 aattagacga ggacgaggaa ttcgaatcta ttgagaaata tattaagaac aggtttaaaa
     4141 aagccgattt aaagtccaaa aagaagagct ccggtatttg ttccaccata ttgatatatt
     4201 gctttaataa ttttgatgat ttgtgtcttc tcagtcaaag actgatccgt atttataccg
     4261 gtgaaagcac tttatacaag ctaaagggaa cgtattattt actcttgacc aaaaactcct
     4321 ggtcagtgga taatacgttc cctt
//
LOCUS       DAOOJC010000191         4344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15799_length_4344_cov_3.602705, whole genome shotgun sequence.
ACCESSION   DAOOJC010000191 DAOOJC010000000
VERSION     DAOOJC010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15799_length_4344_cov_3.602705"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..1296
                     /locus_tag="PLA01_06150"
     CDS             295..1296
                     /locus_tag="PLA01_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPD00915.1"
                     /translation="MKVLFIGGTGIISEAASKQAVKQGIDLYLLNRGQNTEFIPEGAK
                     LVKADIRDKASVNKALKGHTFDAVVDWIAFVPEHVKTDVELFRDKTRHYIFISSASVY
                     QKPLSHYIVTESTPLANPYWQYSRDKIACEEFLMNEYRNTGFPATIVRPSFTYGLKMI
                     PAALNSWNNPWSIVDRMHKGKKIIVHGDGSSLWTMTHNTDFARGLIGLLYNNQSIGHA
                     FHITSDEVLNWNQIYMAIGNAAGVKPEIIHIPSEFIARFSPDATGSLLGDKAVSMVFD
                     NTKIKRFVPGFNATVSFSEGIKETIKWFESHPQKCTVDEEWGYVMDTIISRYEKAIE"
     gene            complement(1332..1877)
                     /gene="lepB"
                     /locus_tag="PLA01_06155"
     CDS             complement(1332..1877)
                     /gene="lepB"
                     /locus_tag="PLA01_06155"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPD00916.1"
                     /translation="MDNLESTGNKKQEIIKEIISWALYILGAVVVALFLTRFVIVNAF
                     VPTGSMETTIMPNDRLIASRIHYLIGNPKRGDIAVFRFPDDEEVLYVKRVIGLPGETV
                     EIKDGTVYIDGTPLEEPYLKEKPIGDYGPYKIPEDSYFMLGDNRNNSKDSRKWNNKFV
                     KKEKILGKALFRYFPSIKALW"
     gene            complement(2097..3026)
                     /gene="fba"
                     /locus_tag="PLA01_06160"
     CDS             complement(2097..3026)
                     /gene="fba"
                     /locus_tag="PLA01_06160"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HPD00917.1"
                     /translation="MPLVSSTEMFKKAYEGKYAIGAFNVNNMEIIQGITEAAKEVNAP
                     LILQVSAGARKYANHTYLMKLIEAAIAETGLPICVHLDHGDTFELCKSCIDGGFTSVM
                     IDGSHHPFEENVKLTKQVVDYAHSKGVVVEGELGRLAGIEDAVNVSEKDAAFTDPDQV
                     QEFVERTGVDSLAIAIGTSHGAFKFKGEPKLRFDILEEVEKRLPGFPIVLHGASSVIP
                     EYVDMINNNGGNMPGAKGVPEDMLRKAASMAVCKINIDSDLRLAMTGTIRKYFAENPS
                     HFDPRQYLGPARSAIKELVKHKIINVLGCDGKA"
     gene            3272..>4344
                     /locus_tag="PLA01_06165"
     CDS             3272..>4344
                     /locus_tag="PLA01_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPD00918.1"
                     /translation="MSRLEGACIFGQSGGPTSVINASAAGVIQEALKQDCITAVYGAA
                     HGIKGILEENFYDMSKEDPYELDLLKTTPSSALGSVRYKLKSAEDDETDYKRLVEVFK
                     KYNIRYFFYNGGNDSMDTCNKVSKYMQKVGYECRVMGVPKTIDNDLWGTDHCPGYGSA
                     AKYIATSTMEVYHDARVYDTGMITILEIMGRNAGWLTAASALAAYKGNGPDLIYLPEL
                     PFDMDKFLEDTMRIYKENGKVIIAVSEGIKDKDGKYISEYGSDMAKTKDSFGHAQLGG
                     LAATLANIVKEKTGAKVRGIEFSLLQRCAAHVGSLTDVNEAYLAGQTAVKLAVEGKTD
                     YMVAFERAEGKEYKCNIKLINLT"
BASE COUNT         1367 a          890 c          804 g         1283 t
ORIGIN      
        1 attacaattg caacgatcaa tattttcttt tttcctttca taaataaaaa cctcctaaca
       61 gctccacctt tgacaataaa attaaatcaa actatagaag aaagagctca gctctgataa
      121 aatacagtta acaacacaga gtaaacccta tatatgtatt ttaaccgata ccctgttaga
      181 acacaatatt aaaggcaact atatatacca atcgatttag tcacaaaatt cagtttggga
      241 acaacaatgc aaattgtatg aacggcaaat aaacaaaggg agaggaatga taaaatgaaa
      301 gtattgttca taggcggaac aggtattatt agtgaggcag cctccaaaca agctgttaaa
      361 caaggtattg atctatacct tttaaacagg gggcaaaaca ccgaatttat acccgaaggt
      421 gcaaaacttg ttaaagccga tatccgggat aaagcatctg tcaataaggc tttaaagggg
      481 catacctttg atgcagtcgt tgactggata gctttcgtcc ccgagcatgt aaaaaccgac
      541 gtcgaacttt ttagggacaa aactaggcat tatatattca taagctcggc atcagtctac
      601 caaaaaccct tgtcccatta tattgttacc gagtcaacac ctctggctaa tccctattgg
      661 caatactccc gggacaaaat tgcatgtgaa gaatttctta tgaatgagta tagaaacacc
      721 ggttttccgg caaccattgt tcgcccttct tttacatacg gacttaaaat gatacctgcc
      781 gcactgaaca gctggaacaa tccatggtcg attgtggacc gcatgcacaa gggtaaaaaa
      841 atcattgttc acggcgacgg ctcatccctt tggacaatga cacacaatac agactttgcc
      901 agagggctta tcggtcttct atataacaat caatccatcg gtcacgcttt tcacatcaca
      961 tccgatgaag ttttaaactg gaatcagatt tacatggcaa ttggaaacgc agccggtgtt
     1021 aaacctgaaa ttatacacat accttctgaa ttcattgccc ggttttcgcc agatgcaaca
     1081 ggaagccttt taggagataa agcagtaagc atggtatttg acaatactaa aataaagcgt
     1141 tttgtaccgg gttttaatgc cacagtatcc ttttctgaag gtatcaaaga gacaatcaaa
     1201 tggtttgaaa gccatcctca aaaatgtacc gttgacgagg aatggggcta cgttatggac
     1261 acaataataa gtagatacga aaaagccata gaataaatat taatgatatc agaataataa
     1321 aggaaattat tctaccatag agctttaata ctgggaaagt atcgaaaaag agccttaccc
     1381 agaatttttt ctttttttac gaacttgtta ttccactttc tagaatcttt ggagttgttt
     1441 ctgttatcac ccaacataaa atagctgtct tccggaatct tatacggtcc ataatcccct
     1501 atcggttttt ccttaaggta gggctcctca aggggggtac catcaatgta tactgtaccg
     1561 tccttaatct ccacagtctc acccggaagt ccaattactc ttttcacata aagaacctct
     1621 tcgtcatccg ggaatctaaa tacggcaata tctccccttt tgggatttcc gattagataa
     1681 tgtatcctgc ttgcaataag cctgtcgttt ggcataattg tggtctccat tgatccggta
     1741 ggaacaaagg cattaacaat aacgaacctt gttaaaaaca atgcaacaac caccgcacct
     1801 aatatataca gtgcccaact gattatctct tttattatct cctgcttttt atttccggta
     1861 gattccaagt tatccatatt acctttcctc ctttacattt ttgataagcc gacagcattt
     1921 atataccttc aacaatatca agtcgaaata actttctgaa aaaaaaatta tcccaccaac
     1981 aaaagctccg ggggtatcga acccccatcg ctttttggtc atttcacttc gtgaaatccc
     2041 actaataaaa agtgaaggtc aattaactga cctccacaga tttatcagac ttcatcttaa
     2101 gcttttccgt cgcagcctaa aacgtttatt atcttatgct taaccagttc cttaattgct
     2161 gaccttgctg gccctaaata ctgtcttggg tcgaagtgtg aaggattttc tgcaaagtat
     2221 tttctgattg ttcctgtcat agcaagtctt aagtctgagt ctatgtttat cttacaaaca
     2281 gccatagaag cagcttttct aagcatatct tcaggaactc cctttgctcc gggcatgttt
     2341 ccgccgttat tgttaatcat atcaacatat tcaggtataa cggacgatgc accgtgaaga
     2401 actatcggga aaccaggaag tctcttctct acttcttcca aaatatcgaa tctcagtttt
     2461 ggttctcctt taaacttaaa tgcaccatgg cttgtaccta tggctatagc aagagaatca
     2521 acgccggttc tttccacaaa ttcttgtacc tgatcgggat cggtaaatgc ggcatccttc
     2581 tcagaaacat taactgcatc ttcaatacct gcgagtcttc cgagctctcc ttcaacaact
     2641 acgccttttg aatgagcgta atcaacaacc tgctttgtaa gctttacgtt ctcttcaaaa
     2701 ggatgatgtg aaccgtcaat cataacggat gtaaatcctc cgtcaataca ggatttacaa
     2761 agctcaaatg tatcaccgtg atccaagtgc acacagattg gaagtccagt ttctgctatg
     2821 gcagcttcaa taagcttcat aagatatgta tggtttgcat actttcttgc acctgctgaa
     2881 acctggagaa tcaatggggc attgacttcc tttgcagctt cggtaatacc ctgaattatc
     2941 tccatgttgt taacattaaa tgcaccaatg gcatatttac cctcataggc tttcttaaac
     3001 atttcagtac ttgatactaa tggcatgttt tcaactcctt atatttaata tatgtgttag
     3061 ttattataac cactcttgtt aagtttttat cataatcatt ttatcagatt catattgaaa
     3121 atcaaggtta aaataaaaaa taataaatat ttttgattca agtacaattg catttcaaat
     3181 tcaattatgt tatattttta tttggttgtg tttaggtaaa tttcaaaatt gaacacatat
     3241 actttacata tttcattaag gaggttttag tatgagccgt ttggaaggtg catgcatatt
     3301 cgggcagtca ggcggtccga catcagttat taacgccagt gcagcaggcg ttattcaaga
     3361 agcattaaag caggattgta ttacggctgt ttatggagca gcccacggta ttaagggtat
     3421 tcttgaagaa aatttttatg atatgagcaa ggaagatccc tacgaattgg atttattgaa
     3481 aactactcct tcttctgcat tgggctctgt aagatacaag ctcaaaagtg ccgaagatga
     3541 tgaaaccgat tacaagagac ttgtagaggt attcaaaaag tataatatca ggtatttctt
     3601 ctataatggc ggaaacgact caatggatac ctgcaacaag gttagcaaat acatgcaaaa
     3661 ggttggttac gaatgccggg ttatgggtgt tcccaagaca attgacaatg acctttgggg
     3721 aacggatcac tgccccggat acggtagtgc ggcaaaatat attgctacat ctaccatgga
     3781 agtttaccac gatgcaaggg tttatgacac cggaatgata accattcttg agataatggg
     3841 aagaaacgcc ggctggctga cagctgcttc agctctggcc gcttataaag gaaacggtcc
     3901 ggatcttatc tatttgccag agttaccctt tgatatggat aaattcctcg aagacacaat
     3961 gaggatttac aaggaaaacg gcaaggttat tattgctgta tcggaaggta ttaaagacaa
     4021 ggacggaaaa tatatttccg agtatggttc cgacatggcc aaaacaaagg attccttcgg
     4081 ccatgcccag ttgggcggtc ttgctgcaac tttagcaaat atcgttaaag aaaaaaccgg
     4141 agcaaaggtt cgcggtatag agttcagtct tcttcaaagg tgtgctgctc atgtaggttc
     4201 cttaaccgat gtcaatgaag cctaccttgc aggtcaaaca gctgtaaaat tagcagttga
     4261 aggaaaaacc gactacatgg ttgcctttga aagagcagaa ggtaaagaat acaaatgcaa
     4321 tatcaagctt ataaacctga caga
//
LOCUS       DAOOJC010000192         4335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15844_length_4335_cov_3.875000, whole genome shotgun sequence.
ACCESSION   DAOOJC010000192 DAOOJC010000000
VERSION     DAOOJC010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15844_length_4335_cov_3.875000"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PLA01_06170"
     CDS             complement(<1..985)
                     /locus_tag="PLA01_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPD00919.1"
                     /translation="MLKELCEKYGVNEEQVRALDIDRNIALKAGAGSGKTRVLTQRFV
                     RCLLENPTLTLDNIAAVTFTKKAAAEMKDRIRKELSRRISSTGDPSEKKRLFDLKMQI
                     TNSNIDTIHGFCAKILRENFAFAGLDPNFGIMEEVDKSIIISEIAQEVIQSFVEEEKN
                     EAVVRFLAQYYSAGFFFGRLEQGIVSAFDAMREKGMDVRSFCREIKTVDINSQDPTEV
                     LERVALVLIGRLQDEYGAYKQKKNLLDFNDLEILTERLLGNEGIGKSYFERFKTIMVD
                     EFQDINPLQKKFLDHLTKKQGMIPEGRLFIVGDHKQSIYGFRGSDYRVFEEA"
     gene            complement(986..4147)
                     /locus_tag="PLA01_06175"
     CDS             complement(986..4147)
                     /locus_tag="PLA01_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPD00920.1"
                     /translation="MKILYYDYFDRNRRGELVKKCIGFMNEGLKAFYILPSREAMFDV
                     RRLFAKEAGNVFNCHVIGFDDLERLVLEEFPGNFKIIGNMEERLILKHVLRELAPGSF
                     FDKVKNKPGFLELLIRTVRKLKRQCVSPEEFSTRTAGLEGNLLVKCQAFYSIYSGYER
                     IKTEKRLMDVDDISIKAANACLKAKLFYNTAVIVVDGFFNIDPVNIKLLENIQKGFPG
                     ISFYANVPYKNENNEQFLKNEVLGDFMKMGFETAEDETDSAAMQNPCFGPIARYVYSS
                     GGEIKADAENFGIFNSPCMDHEIRTFAGTAKELIRNQELKPSDIAVITADTENYRQKM
                     ISIFEEYEIPLCVDRKTRLGSIPVIKDVMALWRLGLADEYPAAFVSVVTSKYMMPSEV
                     LLHEGFTAERMLKLAEEISKRKDKESCFDAFFDEYSNAVENEPAKEAMRKYITAVCTF
                     VKILQEKPLDAVAGFEGFLEGIQIKTNLSYLSREGLIDEKMWLRDIEAVEELIKFFRR
                     LGRAYGEYRLEPKDVEPEGLIRDILDMAGGIEIESTAKETDGVRFFPPDLSRGQVYDT
                     VFVLGVNEGIFPAAGSEAKLFDIAETKRLYDAGIDLGRNEWETEREKLRFNACLAAAS
                     RRLYLSFRTADEDGNVMMASPFIDEAVSVLDKEDREKIVGKPVSMRERVSFKGKISST
                     GEAVKAVNTNLRNSSRIDCGIINPEVQKRLKYPAHAATIEFSREMGKRFDAYDGKLEH
                     PVLAQQDISYGFSVSQLNSYSRCPFTYFAQRVLDIAVDDDIVKEALDIGSFYHEVLSD
                     YHRENQNPCVPDMEKLDAVFYNKVDWLAFDSVPIVLKNLIIEDLLMILRNFITHDAQN
                     MSRYYKATGCLLKPVMLEESFKMKLGEDKGILKGIADRVDLETDSKGGYTGRFIIYDY
                     KKGGIKGIKECIEGKDFQLPLYSRAFNAVLKERFRIENPQCLAILYYSIEKLEWNGII
                     RKDIKKALFEGRKGTRSTPDWANMEVVLSWAEDEAVKVIDNIRKGCFMVPKNCPASSV
                     FGCLYSGMCRYDRTRLARKEGVQ"
BASE COUNT         1141 a         1115 c          756 g         1323 t
ORIGIN      
        1 aagcttcctc aaataccctg taatccgaac ctctgaaccc gtaaatcgac tgtttgtggt
       61 cacccacaat aaacagcctc ccctcgggta tcatcccctg ctttttggtc aggtggtcca
      121 gaaacttttt ttgcaggggg tttatatcct gaaactcatc caccataatg gttttaaacc
      181 gctcaaaata gctttttcca atcccttcgt ttccgaggag tctttcggtc aggatctcca
      241 ggtcattaaa atccagcaag tttttcttct gcttgtatgc accgtattca tcctgcagcc
      301 ttccgataag taccaaggca actctctcaa gcacttccgt tggatcttga ctatttatat
      361 caacggtttt tatttcgcga caaaagcttc ttacatccat tcccttttcc ctcatggcat
      421 caaaggctga tacaatccct tgttcgagcc tcccgaagaa aaaacctgcc gaatagtatt
      481 gggccaaaaa ccgtacaacg gcctcattct tttcctcctc aacaaaagac tgtatcacct
      541 cttgtgcaat ttccgaaatg attatgcttt tatccacttc ttccataatg ccgaagttag
      601 ggtccaggcc ggcaaaggca aaattttccc gcagaatttt ggcacaaaag ccatggatgg
      661 tatcaatatt actgtttgta atctgcatct tcaggtcaaa aagcctcttt ttctcagaag
      721 ggtcaccggt acttgaaatt ctgcgggaaa gctcctttcg gatcctgtcc ttcatttcgg
      781 ctgcagcctt tttagtaaaa gtaacggcag ctatattatc caaagtcaat gtagggtttt
      841 ctaaaaggca ccgtacaaac cgctgggtaa gcacccgggt ttttcccgac cctgctcctg
      901 ccttaagagc tatattcctg tcaatatcaa gggcccttac ctgttcctcg ttcactccat
      961 atttttcgca aagttctttt aacatttact gtacaccctc tttccgtgca agtcttgtac
     1021 ggtcatatct gcacatcccg ctgtaaagac agccaaacac actcgatgcc gggcaattct
     1081 tcggcaccat gaagcaaccc tttcttatgt tgtcaataac tttaacagct tcatcctccg
     1141 cccacgaaag cactacttcc atattggccc agtccggtgt gcttcttgta ccctttcttc
     1201 cctcaaacaa cgctttttta atgtcttttc ggattattcc gttccactcc agcttttcaa
     1261 tactgtaata gagaattgcc aaacactgtg gattctcaat ccgaaaccgt tctttcaaaa
     1321 cagcattaaa cgccctgctg taaagcggca attgaaagtc tttgccttct atgcactcct
     1381 taataccttt gatgccgcct ttcttgtaat cgtaaataat aaatcttccg gtatagccgc
     1441 cctttgaatc cgtttccagg tccaccctgt cggcaatacc ttttaatata cccttatctt
     1501 ctcccagctt cattttaaag gattcctcaa gcataaccgg ctttaacaaa cagcccgtag
     1561 ccttataata tctggacatg ttttgagcat cgtgggttat aaagtttctg agaatcatta
     1621 aaagatcttc tataatcaaa ttcttaagga caatcggtac cgaatcgaag gccagccagt
     1681 ccaccttatt gtaaaaaaca gcatcaagtt tttccatatc tggtacgcaa gggttttgat
     1741 tttcccgatg atagtcactt aacacctcat gataaaaact gcctatgtca agagcttctt
     1801 ttacaatgtc atcgtcaacc gcaatgtcca gaaccctctg ggcaaaatac gtaaagggac
     1861 accgtgaata actgttgagc tgtgatacgg aaaaaccgta gcttatgtcc tgctgtgcaa
     1921 gaaccgggtg ctcaagtttg ccgtcgtagg catcaaaccg ttttcccatc tctcttgaaa
     1981 attctatggt tgcagcatgt gcgggatatt tcagcctctt ttgtacctcg ggattaataa
     2041 taccgcaatc aatccgggaa ctgtttctca agtttgtatt caccgcttta accgcttcac
     2101 cggtgcttga tattttaccc ttaaagctca cccgttccct catgctgacc ggtttcccca
     2161 cgattttttc cctgtcttcc ttgtccaaaa ccgaaaccgc ttcatcaata aaaggagaag
     2221 ccatcatcac attcccgtcc tcatccgcag tcctgaagga caaatatagc ctgcgggaag
     2281 ccgctgccaa gcatgcatta aacctgagct tttcccgctc tgtttcccat tcgttcctgc
     2341 ccaaatcaat tcccgcatca taaagccttt ttgtttcggc aatgtcaaaa agttttgcct
     2401 cactgcctgc cgccgggaat atgccctcat tgacccccaa tacaaaaaca gtatcataaa
     2461 cctgtcccct ggataggtcc ggcgggaaaa acctgacccc gtcggtttcc tttgcagtgc
     2521 tctctatttc aatgccgcct gccatatcaa gtatatcccg aattaagccc tccggctcca
     2581 catcctttgg ctcaaggcgg tattctccat atgcacggcc caggcggcgg aaaaatttta
     2641 taagctcctc tacggcttcg atatcccgaa gccacatttt ttcatcaatc agcccttccc
     2701 ttgagaggta cgaaaggttt gttttgattt gaatcccctc cagaaatcct tcaaagcctg
     2761 ctacagcatc caagggtttt tcctgcagaa tttttacaaa agtgcaaacc gcagtgatgt
     2821 actttctcat ggcttctttt gccggttcat tctccacagc attggaatac tcatcgaaaa
     2881 aagcatcgaa gcagctttct ttatcctttc ttttcgatat ttcttctgca agctttagca
     2941 ttctttcggc tgtaaaacct tcgtgcaaaa gcacttcaga gggcatcatg tacttagaag
     3001 tcaccaccga aacaaaagcc gcaggatact cgtccgcaag tccaagcctc cataaagcca
     3061 taacatcctt tatcaccggt atggaaccca gccttgtctt tctgtcgaca caaaggggaa
     3121 tttcgtattc ttcaaaaatt gagatcatct tctgcctgta attttccgta tccgcagtaa
     3181 taaccgctat atccgaaggt ttgagctctt gatttcgtat cagctctttt gctgttcctg
     3241 caaaagttcg gatttcatga tccatacagg ggctgttgaa tattccgaag ttttcggcat
     3301 cggcttttat ttctccccca ctcgaataaa cataccgggc tatcggccca aaacaagggt
     3361 tttgcattgc agcagaatct gtttcgtcct ctgccgtttc aaagcccatc ttcataaaat
     3421 ctcctaaaac ttcattctta agaaactgtt cgttgttttc gtttttatag ggtacattgg
     3481 cataaaaaga aatgccggga aaaccctttt gtatgttctc caagagcttt atattcaccg
     3541 ggtcaatgtt aaaaaacccg tcaactacaa taactgccgt gttataaaag agctttgctt
     3601 tcaaacacgc atttgcggct tttatggaga tgtcatcaac atccataagg cgtttttctg
     3661 tttttatcct ctcatagccg gaataaatac tgtaaaaagc ttggcatttc acaagcaaat
     3721 tcccttcaag ccccgccgtt cttgttgaaa attcctccgg ggaaacacac tgacgtttga
     3781 gctttcttac ggtacggatt aaaagctcca aaaaacccgg cttgttcttt actttatcga
     3841 aaaaggagcc tggggcaagc tctcttaata catgcttcaa tataagcctt tcttccatgt
     3901 tgccgattat tttgaaattg ccggggaatt cttcaagtac cagtctttca agatcgtcaa
     3961 aaccgataac atggcaattg aaaacgttgc cggcttcttt tgcaaaaagg cgtcttacat
     4021 caaacattgc ctccctcgaa ggcaggatat aaaaggcctt caagccttca ttcatgaacc
     4081 cgatacattt tttcaccagc tctccccgtc tgtttcggtc aaagtaatca tagtacagta
     4141 ttttcataat gcttcactcc aataaaagtt gtctctttaa cattttatca aatttataag
     4201 aaaaatacct ttttttattt caccctacaa atttgggtgt atattattta agtgaggtaa
     4261 aatgcggaag atacattgtg aagcaagttg cagccgactg ttaatacggc atggaaaagg
     4321 agaatattgt atttt
//
LOCUS       DAOOJC010000193         4330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15865_length_4330_cov_3.925380, whole genome shotgun sequence.
ACCESSION   DAOOJC010000193 DAOOJC010000000
VERSION     DAOOJC010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15865_length_4330_cov_3.925380"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..438)
                     /locus_tag="PLA01_06180"
     CDS             complement(124..438)
                     /locus_tag="PLA01_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HPD00921.1"
                     /translation="MRRIGNLSAKVLTSVLAGIMVIGITVAGASAASDTPEITPPQVL
                     KGDINSDGEVNSTDLQYAKRYLLRKIGDGHINFENADMNNDGVIDSTDFSLLKRKILR
                     LI"
     gene            complement(558..1505)
                     /locus_tag="PLA01_06185"
     CDS             complement(558..1505)
                     /locus_tag="PLA01_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPD00922.1"
                     /translation="MYKVRNFAAKMLAVLLVSIAVIGATVTESPAASKTIKIMPVGDS
                     CTAGMGGGNMGSYRTDLYKLLTDAGLSIDFVGSQKGGPSTLPDRDNEGHSGWTIPQIA
                     SNINGWLNTYNPDVVFLWIGGNDIFQTGSINSTGLSNLIDQIFTAKPNITLFVSDYYP
                     WPEIVLPYNAVIPGIVQQKANEGKNVHFVKLSDIQFDRYSDISGDNLHLSETGYTKIA
                     NLWYKYTIDILKSLAGDVSPTPSPTPTPTPTNPPQVLKGDVDSDGEVNSTDLQYLKRV
                     VLRKISIDVINIENADMNDDGEINSTDITLLKRKILRLI"
     gene            1695..2390
                     /locus_tag="PLA01_06190"
     CDS             1695..2390
                     /locus_tag="PLA01_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPD00923.1"
                     /translation="MKILLIEDEIRLCEALGYTLKKNNYIVDTAYDGITGQHMAETGM
                     YNVIILDRMLPGKEGLSVLIELRSQGIATPVIILTAKDSIKNRIEGLDSGADDYLTKP
                     FSKSELLARVRALARRQTETIVNEKINIGNMCFDSLKGEIRTKGHNIKLTSKESQILE
                     LLIKNKNMVISKEQLFERIWGFQSDVELNNIEVYLSYLRKKLARSDCGIVIETIRARG
                     YCLKEVLQYVPQA"
     gene            2374..3585
                     /locus_tag="PLA01_06195"
     CDS             2374..3585
                     /locus_tag="PLA01_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD00924.1"
                     /translation="MFRKLKLKFILTNVVTLTVVLLMIFSGMYFSMRGFLKFQADMIL
                     YTIAEEESLNPNFDPALVRFFFTKVNATGEIIGHLSVITVSTEDMGALKEEVLKRGTA
                     TGKISQGKYKFKFLKVPKEYGFIIVFLDYSVEEIMYKPLITVSTFIALLSIALVFVIS
                     LFLANRSIRPIKTSWEKQAVFIADASHELRTPLAVVNSNLEIVMDNEDKSVGSQKKWL
                     SNIQCELERMKKLIDDLLFLARADADGEMPKEHFDLSELLHKIYDTFTPLFQKKNLEL
                     LLNSPHNIIVCGNEFRIKQLITILLDNAIKYTGEGGKVELTLQTGASTVQLSVKDTGE
                     GIAKEHLGKIFDRFYRVDKSRSRNYGGAGLGLAIAQCIVNEHRGTINVISEVSKGTEF
                     KISLPIENRKQ"
     gene            3776..>4330
                     /locus_tag="PLA01_06200"
     CDS             3776..>4330
                     /locus_tag="PLA01_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HPD00925.1"
                     /translation="MISKYTLPIISKILNLSPVDSRDDIDAYDITILKYNGEPAALAQ
                     TMFFKLCAEGYLEPTYVYRELLFKRTDKSPNNLLPIEQSFLAYFNTPGNLNGLVSRQR
                     NLSILNGFIKELKKRLTYMGFIKSTPKSIIENTIRMALYGIMISLGLTKLFLGISNNK
                     PVKYLLIELIIVSVIFFYVTILSKT"
BASE COUNT         1397 a          808 c          806 g         1319 t
ORIGIN      
        1 cccgtggcta gactttcact agctagatta gtgccatgcc tggcacacag taaaagagta
       61 ccgggaaaag ctatcctccc gatactcttt aagttaagca gttaaaacat taccttatga
      121 aatctatatt agtctaagaa tctttctttt cagcaacgaa aaatctgtag aatctattac
      181 accgtcattg ttcatatctg cattttcaaa atttatatga ccatctccta tttttctcag
      241 taaatacctt ttagcatatt gaagatctgt cgaatttacc tcaccatccg aattaatatc
      301 accttttaaa acttgaggtg gggttatttc cggcgtatca gatgctgccg atgcccctgc
      361 gactgttatc ccgataacca taatgcccgc caacacagat gtcaacactt ttgccgataa
      421 attaccaatt cttcgcatat aattacctcc ctctgaaaaa aaaatatatt tttttatttt
      481 cttaatttta ttataccttt gtgtgagtat ttcatctcac aaaaccatat ttattttaca
      541 taatctcaat aaaaggttta tattaatctt aaaatcttcc ttttcagcaa cgtgatatct
      601 gtagagttta tttcgccgtc atcgttcata tctgcatttt caatattaat tacatctata
      661 gatatctttc ttagtacaac cctttttaaa tattgaagat ctgtcgaatt aacctcacca
      721 tccgaatcaa catcaccttt tagaacttga ggcggatttg tcggagtcgg tgtaggagtt
      781 ggactaggtg tcggagatac gtcgcctgct aaagatttca gtatatcgat tgtatactta
      841 taccacagat ttgcaatttt tgtataccct gtttcgctca aatggagatt gtcacctgaa
      901 atatctgaat atctgtcaaa ttgtatgtcg ctgagcttta caaaatgtac attcttgccc
      961 tcattggctt tttgctgaac tattcccgga attaccgcat tatatggcag aacaatttca
     1021 ggccatggat aataatccga tacaaacaat gttatattag gcttggcagt aaaaatctga
     1081 tctatcaagt tgctaagacc tgttgagttt atacttccgg tttgaaatat atcatttccg
     1141 ccgatccata aaaataccac atccgggttg taggtattaa gccagccgtt gatattgctc
     1201 gctatctgag ggatggtcca cccggagtgg ccttcattat ctctatcggg cagagtactc
     1261 ggtcccccct tttgtgatcc gacaaagtca atactcaaac cggcatctgt taaaagcttg
     1321 tataaatcgg tacggtacga tcccatattt cctcctccca tacccgcggt gcaggagtct
     1381 cccactggca taatctttat tgtttttgat gctgctggtg attccgtaac tgttgcccct
     1441 ataaccgcaa tgctgaccaa cagaactgct aacattttag ctgcaaaatt tctcactttg
     1501 tacatgcaat ctcctccctt gtataaactt atataatatg ttccattatc attctagata
     1561 attaatctta atcttttctt aaaaaagtca tttaaatcaa actttttacc aaatccaaaa
     1621 gataaggcaa taaaaaatca atctgtttcg aacttataat atacaaaata tataacaaac
     1681 agggtgatat ggaaatgaag atattgctga ttgaagatga aatacgtctt tgtgaagcac
     1741 ttgggtatac tttaaagaag aataattaca tagtcgatac ggcttatgac ggaataacag
     1801 gccagcatat ggctgaaaca ggtatgtaca atgttatcat ccttgacaga atgttacccg
     1861 gaaaggaagg actgagtgtc ctcatagaat taagaagcca gggaattgca acacctgtta
     1921 ttattctgac tgcgaaagat tcaataaaaa acagaattga aggtcttgac agcggagcgg
     1981 atgattattt gacaaagcct ttctcaaaga gtgagctgct tgcaagagtg cgggctctgg
     2041 caagaaggca aacggaaaca atagtgaatg aaaaaataaa cattggaaac atgtgctttg
     2101 attccttaaa gggagaaata agaacaaaag gtcataacat aaaacttaca tcaaaagaat
     2161 ctcaaattct tgaattactc atcaaaaaca aaaacatggt gatttcaaaa gaacagctct
     2221 ttgagagaat ttggggattt cagtcggatg tagaacttaa caatattgaa gtatatctct
     2281 cataccttag aaaaaagctt gcaagatccg attgcggaat agtaattgag accatccggg
     2341 caagaggtta ttgcctcaag gaggtattgc aatatgttcc gcaagcttaa gctcaagttt
     2401 atcctgacca atgttgtcac tttaaccgtt gtacttctca tgattttttc cgggatgtac
     2461 ttttcaatga ggggttttct gaaatttcag gcagatatga tactgtatac cattgctgag
     2521 gaagagtctt taaatccaaa tttcgaccct gccctggtca gattcttttt taccaaggta
     2581 aacgctacgg gagaaattat cgggcattta tccgttatta ccgtatcaac ggaagacatg
     2641 ggggcactaa aagaagaggt cctgaaaagg ggaaccgcca ccgggaaaat ttcgcagggc
     2701 aagtacaaat tcaaattctt aaaggttccc aaggagtatg ggtttataat cgtatttctt
     2761 gattacagtg tggaggaaat aatgtataaa ccgcttatta cggtaagcac tttcattgct
     2821 ctgttatcca tagcactggt tttcgttata agtctttttc tcgcgaacag atctattcgg
     2881 cctattaaaa catcctggga aaaacaagct gtttttattg cagatgcatc ccacgaactg
     2941 aggactcccc ttgccgtggt aaattccaat ttggaaatag ttatggataa cgaagataag
     3001 tccgtcggca gtcagaaaaa atggctaagc aacatccaat gcgaactgga acgcatgaaa
     3061 aagctgattg acgatttatt gtttcttgca agagcagatg ctgacggtga aatgcctaaa
     3121 gagcattttg atttaagtga actgctgcac aaaatttatg atacttttac acctcttttc
     3181 caaaagaaga atctggaatt gcttttgaat tccccacata atatcattgt ctgcggcaat
     3241 gaatttcgaa taaagcagct cataacaata ttattggaca acgcaataaa atataccggt
     3301 gagggaggaa aagtggaatt aacactgcaa accggtgcta gtacggttca actgtctgtg
     3361 aaagataccg gtgagggaat agctaaagaa catcttggca aaattttcga caggttttat
     3421 agagttgaca aatcccggtc aagaaattat ggaggtgcgg gattgggcct agctatagcc
     3481 cagtgcatag taaatgaaca taggggaacc attaatgtta taagtgaggt atcgaaagga
     3541 acagagttta aaatatccct gcctattgag aacaggaagc aataatgata gaatttaaat
     3601 gtgagcataa ggcaccgata gagcaagtaa atttatcatt atcaaaagac gaaaatcggc
     3661 tgtgctcaaa agcatcaagt tgaaaggcag gggataagtg ttgttggaat ttattcgaag
     3721 tataccgggg caaacgtttc tgtcaataca tttagtttat tgtgccatat taattttgat
     3781 atcaaaatat acccttccaa taataagtaa aatattaaac ctgtcgccgg ttgattccag
     3841 ggacgatatt gacgcctatg atatcacaat actgaaatat aacggtgaac ctgcagcttt
     3901 agcacagaca atgtttttca agctatgtgc cgaggggtat cttgagccaa catatgtcta
     3961 tagagagttg ctttttaaaa gaacggataa aagtccaaat aatcttttac ctatagaaca
     4021 atcctttctt gcatatttta atacgccggg caatcttaat ggcttggtat cccgtcaaag
     4081 aaacctcagt attttaaacg gttttatcaa ggaattaaaa aaaaggctta catatatggg
     4141 atttattaaa tctactccca agtcaataat tgaaaacaca atccgtatgg ctttgtacgg
     4201 tatcatgatt tcattgggat taacaaagct gtttttagga ataagcaaca acaaacctgt
     4261 taaatatctg ttgattgaac tgataatagt aagcgttata ttcttttatg ttaccatact
     4321 ttctaagacc
//
LOCUS       DAOOJC010000194         4329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_15872_length_4329_cov_3.006083, whole genome shotgun sequence.
ACCESSION   DAOOJC010000194 DAOOJC010000000
VERSION     DAOOJC010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15872_length_4329_cov_3.006083"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..751
                     /locus_tag="PLA01_06205"
     CDS             53..751
                     /locus_tag="PLA01_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPD00926.1"
                     /translation="MDIKLANWMPIESEDYVLSAKDKDEIFKRGAVISIKHPKIFEPV
                     LTVIQKIEGHVLYFRIPEDFLKCNVLKGDAVSCHVMQGDYEYIIDGMIAEFDITYPRL
                     VQLLIKRIRRFKNNRKTKRYLVNFQSFFTPYGIDKKIYAIIKNISLSGVCAVFREEVE
                     TESLINVFVSASIEKTMALEFNAKINRLVERDFYNEYGLEIVEIDDINKDRLDRLIYR
                     LECDEAEFVSSNLK"
     gene            921..1094
                     /locus_tag="PLA01_06210"
     CDS             921..1094
                     /locus_tag="PLA01_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00927.1"
                     /translation="MVIYAVVDRVEKGYAVLICNDCGLEISIPVKDGEKEYAKGDKIS
                     LVLGKDGILRNVG"
     gene            1152..3836
                     /gene="polA"
                     /locus_tag="PLA01_06215"
     CDS             1152..3836
                     /gene="polA"
                     /locus_tag="PLA01_06215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPD00928.1"
                     /translation="MSDKKLMVIDGNSILNRAYYGLNQLLTTSDGTYTNGIYGFINVM
                     QRYIEEENPQYICVAFDLKAPTFRHAMYEGYKAKRKGMPEELRVQVPLLKEVLDAMSI
                     KRLEKEGYEADDILGSISLCAENNGVEVILVTGDRDAFQLVGPSTKLKLPRTKGGKTI
                     TEEFDYKRIVEVYGIEPRQFIDVKALMGDASDNIPGVPGIGEKTALDLIKEFNSLDNL
                     YDSLDSVTKKSVREKLETYKEQAYMSRDLATIDRNVPSLCDFEELKRREIDREKTYEI
                     FKRLEFKSFIEKFGLIDAHHGDSFGADIQIAKSNDELEELKDSISASKKAYIYYLLDR
                     TGKFSKQLTALAVSSGEDRVWYVDFVNYIDEDKFLSRFKEILENPGIEKYGHDFKNFI
                     VYLNTKGIELQGLAFDTMIGAYIINPSRETYTLSEIASEFLGLSVESVEMLSGKGKNF
                     TLFREMPVLNLSKVVGNYPCVIDRVSQKIDDMLDENGQKDLYFNIELPLVKTLADMEY
                     NGFKVNVHGLVEFSRELDVKIEEIKSRIFSLAGEEFNINSPKQLGVILFEKLGLPVVK
                     KTKTGYSTDAEVLEELSDRHEVIGEILQFRQLMKLKSTYAEGLLSVINPETGRIHSSF
                     NQTVTVTGRISSTEPNLQNIPIKIEMGRKIRKVFIPSSDDFQLLDADYSQIELRVLAH
                     ITGDENMINAFLNNEDIHASTAAKVFDIPVEEVTSTMRSRAKAVNFGIIYGIGDFSLA
                     KDLGITRKEARKYIDGYLDRYPKVKQYMHDIVEKGRTQGFVTTMFNRRRYLPELKSRN
                     FNIRSFGERVALNTPIQGSAADIIKIAMVKVHEELKKRQLKSRLILQVHDELIIEVFK
                     DEKEEVVKILKDGMENAVSLKVPLVVEVKSGNNWYETK"
     gene            3880..>4329
                     /gene="coaE"
                     /locus_tag="PLA01_06220"
     CDS             3880..>4329
                     /gene="coaE"
                     /locus_tag="PLA01_06220"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520919.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPD00929.1"
                     /translation="MKTIGVTGGIGSGKSTVSKILSDLGAEVIDADAIYRCIAKKGNE
                     VYREIVESFGDEILDVNGEIDRKKLAAIAFKDEDGIRTLNSVTHKHVARIIIEKLNEF
                     KNREKVVVIDAAIPVEHGFLDVADQVWVVLADKENRIKRVMKRSGLTY"
BASE COUNT         1460 a          632 c         1052 g         1185 t
ORIGIN      
        1 ttttaatttt tactctcaga tccaactcaa taaagccgaa ggagggaata tcgtggatat
       61 caagctagct aactggatgc ccatagaaag tgaagactat gttttatctg caaaagacaa
      121 ggacgaaatc tttaaacggg gggctgttat cagcatcaag catccgaaaa tctttgaacc
      181 cgttttaact gtgatacaaa agatagaagg gcacgtattg tatttcagaa tacccgagga
      241 tttcttaaaa tgtaatgttc taaagggtga tgccgtatct tgtcatgtta tgcagggaga
      301 ttatgaatac attatagatg ggatgattgc tgagtttgat atcacttatc ccaggcttgt
      361 acagttgctg ataaaaagaa taagaagatt taagaacaat agaaagacta aaagatacct
      421 tgtaaacttt cagtcattct ttactcccta tgggattgat aaaaaaatat acgccataat
      481 aaaaaacatc agtctatcgg gtgtatgtgc agtttttcgg gaagaggttg aaactgagag
      541 cctgattaat gtttttgtca gtgcttcaat tgaaaaaacc atggctctcg agtttaatgc
      601 gaaaataaac aggctggtgg aaagagattt ttataacgaa tatggtttgg agattgttga
      661 gattgatgat ataaataagg acaggctcga tagactcatt tatcgtctgg agtgcgacga
      721 agcagagttt gtttccagta atttaaaata ataattaaga aattcaaaaa cagctgttta
      781 cggctgtttt ttttttgtgt acaatattta aggagaatag tttgtgtact tatccgacag
      841 gataaaaaaa acatatatta ataaagactt taccttatga aaggtgaagt ctcggaaaat
      901 ttgacaaagg tgatgaaaag atggttatat atgcagtagt agacagagtt gagaagggat
      961 atgcagtttt gatatgtaac gattgcggtc ttgagattag tattcctgtt aaagatggag
     1021 aaaaggaata cgcaaaagga gataagattt cattggttct aggcaaggac ggtatcttga
     1081 gaaatgtcgg gtaaataact cgagcgagaa aacttattaa ttttagtgaa acaataagcg
     1141 agaggttaat aatgagtgat aaaaaactta tggttataga cggaaacagc atacttaaca
     1201 gggcatatta cgggctgaat cagcttttga caacatctga cggaacctat accaatggaa
     1261 tttacggctt tataaatgtt atgcaacggt atattgaaga agaaaatccc cagtacatat
     1321 gtgttgcctt tgacttgaaa gccccaacct tcagacatgc catgtatgaa ggatacaagg
     1381 caaaaagaaa aggcatgccg gaagaactca gggttcaggt tcctctttta aaggaagtct
     1441 tggatgcaat gagcataaag aggcttgaaa aggagggcta tgaggctgat gacatactgg
     1501 gttccatctc tttgtgtgct gaaaataacg gagttgaagt tatcctggtt accggagaca
     1561 gggatgcatt tcaactggtg ggaccgtcaa caaagctgaa gcttcccaga acaaaaggcg
     1621 gaaagaccat tacggaggag tttgattaca aacggattgt tgaggtatac ggaatagaac
     1681 cccggcaatt tattgatgta aaggcattaa tgggtgatgc ctccgataat attcccggcg
     1741 ttccgggtat cggtgaaaaa acggctttag atctcattaa agagtttaat agccttgata
     1801 atttatatga ctctttagat tcggtgacca aaaagagcgt gagggaaaag cttgaaactt
     1861 ataaggaaca ggcttatatg agcagggacc ttgccacaat tgacagaaat gtaccgtctc
     1921 tttgcgattt cgaagagctc aaaagaaggg aaattgacag ggagaagact tatgagatat
     1981 ttaaaagact tgagtttaag agctttatag aaaagtttgg gttaatcgat gcacatcatg
     2041 gggacagttt tggagctgac attcaaattg caaaaagcaa tgatgagctt gaagaattga
     2101 aagattcaat atctgcgtct aaaaaggcat atatatacta tttacttgac agaacaggaa
     2161 agtttagcaa acaacttacc gccttggcag tttcgtctgg agaagaccgg gtgtggtatg
     2221 ttgattttgt caattacatt gatgaagata aattcttaag ccggtttaag gagattcttg
     2281 agaatcccgg tatagaaaaa tacggccatg atttcaaaaa ctttatagtt tatctgaata
     2341 ccaaaggaat agagcttcaa ggcctggctt ttgacaccat gataggtgca tatataataa
     2401 atccttcaag ggaaacatat actttatcag aaatagcttc agagtttctc ggcttaagcg
     2461 tggaatcggt tgaaatgctt tcaggaaagg gtaagaattt cactttattc agggaaatgc
     2521 cggttttgaa cctttcaaag gttgtgggaa attatccctg tgttatagac cgggttagtc
     2581 agaagataga cgatatgctt gatgagaacg gtcagaagga cctttatttc aatatagagc
     2641 ttcccctggt taagacactg gcagacatgg agtacaacgg atttaaggtt aatgttcacg
     2701 gattggtgga gttttcgagg gagttggacg taaagattga ggaaataaag agcaggatat
     2761 tctctctggc aggtgaagag tttaatatta attcgccgaa gcaactggga gttatccttt
     2821 ttgaaaagct ggggcttccg gttgtaaaga aaacaaaaac cgggtattct accgatgcgg
     2881 aggttttaga ggagctttcc gaccgccatg aagtgattgg ggagatactt caattcaggc
     2941 agctaatgaa gctgaagagc acttatgccg aagggttgct gtcggtaata aaccctgaaa
     3001 cgggcaggat acattccagc ttcaaccaaa cggtgacagt aaccggaagg ataagcagca
     3061 ccgaacccaa ccttcagaat attcctataa agattgagat gggaaggaag ataaggaagg
     3121 tgtttatacc ttcaagcgat gatttccagc tccttgatgc cgattattcg caaattgagc
     3181 tcagggtttt agcccacata accggggatg aaaacatgat aaatgctttt ttaaacaatg
     3241 aggatataca tgcatccaca gcggcgaagg tctttgatat acccgttgaa gaggttacat
     3301 caaccatgag gtcgcgggct aaggccgtta atttcggtat tatttatggg ataggtgatt
     3361 ttagtcttgc aaaggatttg ggcataacaa gaaaagaagc gagaaaatat atagacggat
     3421 atcttgacag gtatccgaag gtaaaacagt atatgcatga tatagtagag aagggaagaa
     3481 cccaaggttt tgtaaccact atgttcaata gaagaaggta tcttccagag ttgaaatcgc
     3541 gaaacttcaa tatcagatcc tttggagagc gtgttgcttt aaatacacct atacagggaa
     3601 gtgctgcaga tataattaaa attgcaatgg tgaaggttca tgaagaactg aaaaagagac
     3661 aattgaaatc ccggttgata ttgcaggtgc acgatgaact gataatagaa gtgtttaagg
     3721 atgaaaagga agaagttgtt aagattctga aggatggcat ggagaatgct gtcagcctga
     3781 aggttccgtt ggttgtcgag gttaaatcgg gcaataattg gtatgagacc aagtgaataa
     3841 cttttatata aaataaaaaa gtaaaaagga aggttgcatg tgaaaacaat aggagttacc
     3901 ggaggaatag ggagcggtaa gagtactgtg tcaaagattt tgtccgactt gggggcagaa
     3961 gtaattgatg ctgatgccat atatcggtgt atcgctaaaa aagggaatga agtctatagg
     4021 gagattgtgg aatctttcgg tgatgaaata cttgatgtta acggtgagat tgacaggaaa
     4081 aagcttgcag ccatagcttt taaagatgaa gacgggatca ggacattaaa cagtgttacc
     4141 cataagcatg tagcccggat aattattgaa aaacttaatg aatttaagaa cagggaaaag
     4201 gttgttgtta tagatgctgc aataccggta gagcatggtt ttcttgatgt tgccgatcag
     4261 gtatgggttg tattggcaga caaggaaaac aggattaaga gggttatgaa aagaagcggt
     4321 ttaacctat
//
LOCUS       DAOOJC010000195         4295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16043_length_4295_cov_4.811792, whole genome shotgun sequence.
ACCESSION   DAOOJC010000195 DAOOJC010000000
VERSION     DAOOJC010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16043_length_4295_cov_4.811792"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..733)
                     /locus_tag="PLA01_06225"
     CDS             complement(8..733)
                     /locus_tag="PLA01_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD00930.1"
                     /translation="METVLKVIGITKKYNNGRGIFDINLDIQRGDIFGFLGPNGAGKT
                     TAMKIMTGLMSADSGDVEIFGHSITKEFEKAMEKVGCIIETAESYAYLSAYENLKLFS
                     RFYDDVDNNRIDEVLELTGILKYKKEKVKNFSLGMKQRLGIASAILSRPELVILDEPL
                     NGLDVQGMIEIRQLIKMLAETDKTTFFISSHLIHDVELTCDRIGILYNGRLVNVDSRE
                     NILRNYASLENCYVSEVGKNGNV"
     gene            complement(962..2215)
                     /locus_tag="PLA01_06230"
     CDS             complement(962..2215)
                     /locus_tag="PLA01_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00931.1"
                     /translation="MRPKKRLMIILSFCLGAALFMSTAFADVLSKEGYDQFKDAIKNT
                     ATRIIEDYDSYTSEVAISVKDNDEVLMSNTLVSKMDSANGKSETISTEEYGKGKKKSY
                     YTYEDTTSSIRKDSDQSVYYVSEYQIEREVGNTHNPFEDEEFEDIEKIFDALIGSLKN
                     HVIVEENNDGTKEFSGTISDTQIPALMNAITSFCFKQAVSGSEFDDNVSIPYLKDDVF
                     IDSISGSASVNMDGIIDNLFASFILSGRDEDGVEHELTFEAVMKLYNFNSTAVTKPDL
                     TGKEVEKDIIKNIPGINISNKYLGKWKNDILIDENDSYVKIGERFVEITAIDDQYVHG
                     RYYEVYKEEYAEYADEGNKEFEFKIDTNEDMSRFEGVYTSDDSVSGYIWFDDGYVELI
                     IDYNSYRSDKPYKNFNRQFNKVFEE"
     gene            complement(2345..3949)
                     /locus_tag="PLA01_06235"
     CDS             complement(2345..3949)
                     /locus_tag="PLA01_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HPD00932.1"
                     /translation="MKKQSLTKGFAVLSAAGMITKVLSVLYIPFLLAIIGEEGNGIYA
                     AAYQVYVFIYVIANSGIPVAISKSVSELTAVGNYKDAVRTFRIARFFLIVIGLVLTIL
                     MFVLAKPLASMINADKSALAIAVLSPTLFFTALASAYKGYFQGMSNMTPTAVSQVVEQ
                     IFNVIFTITFAAVLIKISLEASCAGATVGTSLGALFSAIVLIIMYNGKQKTNVLAEHK
                     STVRRHTYRELAEKVIYYSIPITICVGAQYAGNLIDVANIRGRLSAAGYTIEMVSVMH
                     SYLSKYQQIMNAPISIVSALAAAVLPSISAAAAEGNFKLMEEKTKQAFRLCLLIVFPS
                     AVGLSVLSQPVYSLLKYGEGAYLMRYGSVVLILMSIVQIQSSILQGAGKMYRATINII
                     LGIVLKLVLNYLLISNPNINIMGVVVSSIAGFGLSVVLNTITIRKQARIKTNIVNQSI
                     KPFISSVVMGAAVWIVYGGVYRVLGFMGSSYVANAVAALISVFAGMIIYFLVMIKVHG
                     ITNDDLQVLPERIKRVIPRQVLERVR"
BASE COUNT         1281 a          967 c          661 g         1386 t
ORIGIN      
        1 tccagcttta aacgttccca tttttgccta cctcgcttac ataacagttt tccagtgaag
       61 cataatttct taggatattt tccctactat caacattgac aagccttccg ttgtagagta
      121 ttccaatcct gtcacatgta agctccacat catgaataag gtgactggaa ataaagaaag
      181 tagttttatc ggtctctgcc agcatcttta tcagctgacg aatttctatc atcccctgga
      241 catcaagtcc gtttaaaggt tcatccagta ttaccagttc gggccttgac agaatcgcgg
      301 aggcaattcc aagcctctgc ttcatgccca gcgaaaaatt ctttaccttt tcttttttat
      361 attttaaaat gcctgtcagt tcaagaactt catcaattct attattgtcc acatcatcgt
      421 aaaaccttga gaaaagtttt aaattctcat aggccgaaag atatgcatag gactcggcag
      481 tctcaattat acagcctacc ttctccatgg ccttttcgaa ttctttggta atactgtggc
      541 caaaaatctc tacatctcca ctgtcagctg acataagccc ggtcattatc ttcatcgccg
      601 tagtctttcc cgcaccgttc gggccaagaa aaccaaagat atctcccctt tgtatatcca
      661 ggtttatatc gaaaataccc ctgccgttat tgtatttttt tgtgattcca attactttaa
      721 gtactgtttc cattaatgtt cctccaacta tataataaag ctaaaaaaat catactcccc
      781 gctattttgc tattcagcca agtttttggg ttaaatcgtt atcaatctat aaaatgctaa
      841 aatacacttt atatgttccg accttgattc tcaaagttaa tatctataca ggataccggt
      901 ataaagccgg cagcagcgaa ctctgccacc ggctctaaag cagtcactgt accaattgta
      961 tttactcctc aaaaaccttg ttaaactgac ggttgaaatt tttgtaaggt ttatccgacc
     1021 tgtatgaatt gtagtcaata atcagttcaa catatccgtc gtcaaaccaa atatatcctg
     1081 atactgaatc atctgaagta taaacgcctt caaatctcga catatcctca tttgtatcta
     1141 ttttaaactc aaattcttta ttaccttcgt cagcatattc tgcatactct tccttatata
     1201 cttcataata tcttccatga acatactgat catctatagc tgtgatttca acaaaccttt
     1261 ctccgatctt aacataactg tcattttcat caatcaaaat gtcattcttc catttaccca
     1321 agtatttatt tgatatattg atccccggaa tgttctttat tatatctttt tctacttctt
     1381 ttccggtaag gtcaggtttg gttaccgcag tagagttaaa gttgtagagc ttcattactg
     1441 cttcgaaagt caattcgtgc tcaactccgt cttcgtccct tccggacaat ataaaggaag
     1501 caaaaagatt atcgattatt ccgtccatgt tcacactggc acttccgctt atggaatcaa
     1561 taaatacgtc gtctttaaga taaggtatgg atacattatc gtcaaactcc gatcctgata
     1621 ctgcctgttt gaagcaaaac gaagttattg cattcatcaa cgcaggaatc tgtgtatcac
     1681 ttatagttcc ggaaaactcc ttagtgccgt cgttattctc ttcaactata acatgatttt
     1741 tcaggcttcc aatcagagca tcaaatatct tttcaatgtc ttcaaattct tcatcctcaa
     1801 aggggttgtg agtattacca acctctcttt caatttgata ttcagacacg taataaacac
     1861 tctgatctga gtcttttcta atactgctgg tagtatcctc ataggtatag taacttttct
     1921 tctttccttt tccatactct tcagtactga tggtttccga tttgccattt gccgaatcca
     1981 tctttgatac taatgtattc gacatcagta cttcatcatt gtctttgacg gaaatagcta
     2041 cctcactggt atagctgtca taatcctcaa taattctcgt agctgtgttt tttatcgcat
     2101 ccttgaactg atcatacccc tccttggata gaacatccgc aaaagctgta ctcataaaca
     2161 atgcagcacc caaacagaag cttaaaatta tcatcaatct cttttttggt ctcattccac
     2221 tctctccttt ttgtaggttg atataacaaa atcaaaggaa acatttcctt acttactatt
     2281 ttacacaata cctataaaat tcaaataaat aatatataaa caaattataa atctttataa
     2341 actgttacct tactctctcc aatacctgcc ttggtataac tctcttaatc cgctccggta
     2401 aaacctgcaa atcgtcattt gtgattccat gtacctttat catcacaagg aaataaataa
     2461 tcattccggc aaaaacagat attaatgcgg ctacagcatt agcaacatag ctgctgccca
     2521 taaaaccaag tacccggtag actccgccgt acacaatcca caccgctgct cccataacaa
     2581 ctgatgaaat aaagggcttt attgactgat ttactatatt tgtttttatc cgtgcctgct
     2641 ttcttatagt tatggtgtta agaacaactg acaggccaaa ccctgcaata ctgctgacca
     2701 ctactcccat aatatttata ttgggattgc tgataagaag atagtttaaa accagtttca
     2761 aaacaatccc cagtattata tttattgttg ccctgtacat ttttccggct ccctgtaaaa
     2821 tcgatgactg tatttgcaca attgacatca ggattaaaac cacagaaccg taacgcatga
     2881 gatatgcccc ttcgccatac ttcagcaaag agtataccgg ctgactcaac accgacagtc
     2941 ccactgccga tggaaacact ataagcaagc agagtctgaa agcttgcttt gttttttcct
     3001 ccatcagttt aaaattgcct tccgcagcag ctgcggaaat ggacggaagt accgctgccg
     3061 ccagtgcgga aactatagaa atcggtgcat tcattatttg ctggtatttg gacaaatagc
     3121 tgtgcattac tgaaaccatt tcgattgtat atcctgctgc cgacagcctt ccccttatat
     3181 tggcaacatc aatcagattt cctgcatatt gggctcccac acagatggtt atcggtatgc
     3241 tataatatat gactttttct gcgagctccc tgtaggtatg ccttctaact gtgcttttat
     3301 gctcagccag tacatttgtt ttttgttttc cattgtacat aataattaag actattgcag
     3361 aaaacagtgc ccccagggaa gtaccgacag ttgctccggc acaggaagcc tccagactta
     3421 tttttattag aacggctgca aaagtaatag taaaaataac attaaatatc tgttctacca
     3481 cttgggatac agctgtcggt gtcatgttgc tcatgccttg aaaataacct ttgtacgcgg
     3541 acgccaaagc agtaaaaaac agagtcggag acaatacagc aatagcaagg gcagatttat
     3601 cggcattaat catactggcc aacggctttg ccaatacaaa catcaatatt gttaaaacaa
     3661 gaccgattac aatcaagaaa aaccgtgcta ttctaaatgt tctcactgcg tctttgtaat
     3721 tacctacagc cgtaagctcg gatacggact ttgaaatagc caccggaata cccgaattgg
     3781 ctattacata gataaacaca taaacctggt acgcggcagc gtaaattcca tttccctctt
     3841 caccgataat agccagtaga aaaggtatat acaatacgga caatactttt gttatcatac
     3901 cggctgccga taatactgca aaaccttttg tcaatgattg tttcttcata aattctccta
     3961 tgctttataa aaggcggtac catacccttt ttgaatgttt tcgcaccacc gtactttaat
     4021 ctatattcta atataatgat atatcttatt caataccttt aatttggctg gagaatatag
     4081 caaaacctca acgacaatcg aagctttttt ctaccggcat tgcctgtttt ttttgataaa
     4141 attgctgtta tattacggat tataagtttg attgttggtg aacaacctta tactataaaa
     4201 agtatgccga aagacaaaag gaatctgttt atatatacgc tatataaaaa taaaggattg
     4261 tcaaaaagta tgatcgagag ggctactgga atatc
//
LOCUS       DAOOJC010000196         4295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16046_length_4295_cov_3.266274, whole genome shotgun sequence.
ACCESSION   DAOOJC010000196 DAOOJC010000000
VERSION     DAOOJC010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16046_length_4295_cov_3.266274"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLA01_06240"
     CDS             complement(<1..357)
                     /locus_tag="PLA01_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00933.1"
                     /translation="MSYELGMQAVNLEMPDIVPRTEYSYQLAYELLHAVTGIAVNKDS
                     DDDTKFNAITAFERAWDISLFWATGIGSNIFGDKRTTMGHAGFEEGYGDYRDNKHEAF
                     TDIEEIYNLDFYETYGT"
     gene            532..1452
                     /locus_tag="PLA01_06245"
     CDS             532..1452
                     /locus_tag="PLA01_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HPD00934.1"
                     /translation="MTTLIAGLIISIALLSVIVYFNSKKDSKKKFRANCIVAALPLII
                     AFFIASIAVIPANSVGVQYSPFKGVLEETLPEGWHFKGVFDNIYIISTEVQTSTLTEI
                     TGQTKDSQYVEMVIDVKYKVSPEKAYEVFKQYRTLENVAVSLVSPTVQRSIETISIQY
                     NIIEILGEKRNELYAGIEEELTKRFAINGIQFVSINFTDTDAGAEIEKAIQDEAIAKK
                     RVETAEQERLRVEIEAQQRVIEASANKEKAEIEAQIKLIEAQAEAEANRLIAESITQE
                     LLQKMEMEARLKWGWVTVQGGSVITDIREK"
     gene            1495..1959
                     /locus_tag="PLA01_06250"
     CDS             1495..1959
                     /locus_tag="PLA01_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00935.1"
                     /translation="MEFGWINVFGAGIVLFLMIPNIVYAVKNKNEKNVCTDKYMNITE
                     QIGRYACIILMWLPLLVWKFGFASVFEMLLYIIGNGVLLIAYWSVFALYLKKKNKNRA
                     LILAILPACIFFFSGLLLRHWLLVGFSVIFAIGHIYVTLKQFSPTTTVQNDK"
     gene            1949..2461
                     /locus_tag="PLA01_06255"
     CDS             1949..2461
                     /locus_tag="PLA01_06255"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HPD00936.1"
                     /translation="MTNRKMTKHQTVTHPLQPLFNDDSKILILGSFPSVKTREYGFFY
                     GHPKNRFWPLIERIFDVKLSTDIEERRTFLLQHHIAVYDVIYQCDIIGSGDSSIQNVI
                     PSDLSRIFKEADIKKVFCNGATAYHYYQKYQEQKTNAKAIQLPSTSPANARYSMDDLY
                     NQWKVINYIL"
     gene            2658..3062
                     /locus_tag="PLA01_06260"
     CDS             2658..3062
                     /locus_tag="PLA01_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009537151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HPD00937.1"
                     /translation="MKYVYPAIFTVEEDGGYSVNFPDFESCYTCGDTLAESLAMAEDV
                     LAFTLYKLETEQALLPAPGKLQGLSLGENEFANYIACDTMEYRKRKNSKAVKKTLTIP
                     SWLNEEGIARGINFSQVLQDALMEKIEKYTKT"
     gene            3147..>4295
                     /locus_tag="PLA01_06265"
     CDS             3147..>4295
                     /locus_tag="PLA01_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009535117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HPD00938.1"
                     /translation="MKTYLPRIIDKLLKERLEAKGAVLLEGPKWCGKTTSAKEIAASF
                     ISIDQPEMTKQYQSMAELSPLQLLEGETPRLIDEWQIAPNLWNAVRYEVDQRDEFGQF
                     ILTGSAVPAEIDKSMHTGTGRISRLYMRPMSLYESLDSSGKVSISDLFASREISAKSD
                     TDLEEIAFLICRGGWPKSVGLPENPALYQARDYFEAVVSTDISRVDSVRRDKEKAKRI
                     LRSYARNVGTKTSLETIRQDVLANHAGTLDPVTLYSYRDALTKIFVIEDSPAWNPNLR
                     SKVAIRTTDTRYFVDPSIAAAALELGPVDLLNDLSTMGLIFENLCVRDLRIYADYLDG
                     SIYQYRDSNGLECDAVIHLRNGSYGLIEIKLGGDSLIEEGVNNLNKLAS"
BASE COUNT         1417 a          801 c          907 g         1170 t
ORIGIN      
        1 tgttccgtat gtttcataaa aatccagatt atatatttcc tcaatatcgg taaaggcctc
       61 atgcttatta tccctgtaat ccccgtaacc ttcttcaaat cccgcatgcc ccatagtggt
      121 ccttttatct ccgaagatat tactgcctat tccggttgcc cagaaaagtg atatatccca
      181 tgctctttca aaggcggtaa ttgcattgaa ctttgtatca tcatcggaat ccttattgac
      241 ggctattccc gtaaccgcat gtaaaagttc gtatgccaac tgataagaat attcggttct
      301 tgggactata tcgggcattt ccaggttaac agcctgcatt cctaactcat atgacataca
      361 aaatcctcca taacctattc tatcattatt actgatgttt acaaggtata agcatttgac
      421 ttgataaggg taaagatata atattttatg gaaagactga tgtttataag ttacttaaaa
      481 gtaccgacac ttatcagtaa caaacgatat aaaagaaaag gagtatgtga catgacaaca
      541 ttgatagcag gactgataat ctcaatagct ttattaagcg tcatagttta tttcaacagc
      601 aaaaaggaca gcaaaaagaa attccgtgca aactgtatag ttgccgcact tccgctcata
      661 atagcatttt tcattgcctc aatagcggta attccggcaa actcggtagg tgtacaatac
      721 agcccgttca aaggagttct ggaggaaaca cttcccgaag gatggcattt taaaggcgta
      781 ttcgacaata tatatataat cagtaccgaa gtgcagacca gcaccctcac tgaaattacc
      841 ggtcagacaa aagacagcca gtacgtcgaa atggttattg acgttaagta taaggtttct
      901 ccggaaaaag cctatgaagt gtttaaacaa tacagaactt tggagaatgt cgcagtaagt
      961 ctggtatcac cgaccgtaca aagaagtatt gaaaccattt ccatacaata caacattatc
     1021 gaaatattgg gagaaaagcg aaatgaactt tacgcaggca tcgaagagga acttacaaaa
     1081 cgttttgcaa taaacggtat acagttcgta tccattaact ttaccgatac cgatgccgga
     1141 gcggaaattg aaaaagctat tcaggatgaa gccatcgcca aaaaacgtgt ggaaacggcg
     1201 gaacaggaac gtctgcgtgt ggaaatagaa gcccagcaaa gagtaataga ggcatcggca
     1261 aacaaggaaa aggcggaaat agaggcccag attaaactga ttgaagcaca ggccgaagcc
     1321 gaggccaaca gacttatagc cgaatccata acacaggaac tgttgcaaaa aatggaaatg
     1381 gaagcccgtc ttaaatgggg atgggtaacc gttcagggcg gttcggtaat aacggatata
     1441 agggagaagt aataatccgc tattcaagaa tcggaatttg ttaaggagaa ttgtatggag
     1501 ttcggatgga ttaatgtgtt cggagcgggt attgtgcttt ttcttatgat accgaacatt
     1561 gtttatgccg taaaaaacaa aaatgagaaa aatgtttgta ccgataaata tatgaacata
     1621 acggagcaga taggaagata tgcttgcatt atcctgatgt ggctgcctct tcttgtttgg
     1681 aaattcggat ttgcaagtgt atttgaaatg ctcctttata tcataggtaa cggcgtactg
     1741 ctgattgcat actggtcggt atttgcacta tacttaaaaa agaagaacaa aaaccgtgca
     1801 cttatcttag ccatattgcc cgcatgcatc tttttcttta gcggactgtt attgcgacat
     1861 tggctgcttg tgggtttttc cgttatattt gcaatcggac acatttatgt aacattgaaa
     1921 cagtttagtc cgacaactac cgtacaaaat gacaaataga aaaatgacca aacaccaaac
     1981 cgttactcat ccgttacaac ctctttttaa tgacgactca aaaatcttaa tcttgggttc
     2041 ctttccctcc gtaaaaacca gagaatacgg cttcttttac ggacacccga agaatcgttt
     2101 ctggcctttg atcgagagga tttttgatgt gaaactgagt acggatattg aagaaagaag
     2161 aacctttctt ttgcaacatc atattgccgt ctatgatgtc atttatcaat gtgacattat
     2221 agggtcaggt gattccagta tccaaaatgt aatcccctcg gatttaagcc gaatctttaa
     2281 agaggcggat ataaagaaag ttttctgtaa cggagcaacc gcttatcact actatcagaa
     2341 atatcaggag caaaaaacta atgctaaagc tattcaactt ccctctacca gccctgccaa
     2401 tgcaagatat tccatggatg atttgtataa tcaatggaag gtaataaatt atatactttg
     2461 acatacgtat taatacgtgt tattatatag ttgccgaaag gaatgtgcct gtgaaagtat
     2521 ctgaactcat taaaaaactg aaggcaagta agaagtgtca tctcatcgaa cacggtgcaa
     2581 gacacgacat gtggcatagc gacataaccg gaaaaaactt ccctgttcca aggcataata
     2641 gttaggagat taattctatg aaatatgtat atccggcaat attcacagtt gaagaagatg
     2701 gaggctattc cgtaaacttt ccggactttg aaagctgtta cacatgtggc gatacattag
     2761 cggaaagcct tgctatggcg gaagatgttc ttgcctttac actctacaaa ctggaaacgg
     2821 aacaagcttt gcttcctgct cccggtaaac ttcaagggtt aagtctcgga gaaaacgagt
     2881 tcgccaacta tatcgcatgc gatacaatgg agtaccgaaa acgaaagaat agcaaagctg
     2941 tcaaaaaaac actcacaatc ccttcatggc taaacgaaga aggaattgca agaggtatca
     3001 atttttctca agttttgcag gatgctctaa tggagaaaat agaaaaatat acaaaaactt
     3061 gaatagaaat ccctcaaaca cttgaataaa acttcctcaa acacttgaat atgtgctata
     3121 atgtccttgt ttagtgaggt aggcttatga agacatattt accgaggatt attgataaat
     3181 tattaaaaga aaggttggag gctaaaggag ctgttctatt ggagggacct aaatggtgcg
     3241 gtaagactac cagtgctaaa gaaatagcag ccagctttat atcaatagac cagcctgaaa
     3301 tgaccaagca gtatcagtca atggctgaac tctctccgtt gcagcttttg gaaggagaaa
     3361 ctccgaggtt aatagacgaa tggcaaatag cacctaattt gtggaatgca gtaagatatg
     3421 aggtggatca aagagatgag ttcggtcaat tcattttgac aggttcagct gttcccgcag
     3481 aaatagataa gtctatgcat acagggaccg gcaggatttc aagactatat atgcgcccca
     3541 tgagtttata tgaatctttg gattcaagcg gtaaagtatc tattagcgac ttgtttgcaa
     3601 gtcgggaaat atccgctaaa agcgatacag acttggagga aatagcattt ttaatatgca
     3661 ggggcggttg gcctaaatct gtcggattac ctgaaaatcc ggctctttac caagccagag
     3721 actactttga agctgtcgtt tcaaccgaca taagcagagt ggactcggta agaagagata
     3781 aggaaaaagc aaaaagaata ttgcggtcat atgcaagaaa tgtgggaacc aaaacctctt
     3841 tagagactat aaggcaggat gtattggcaa atcacgcagg caccttggac cctgtgacac
     3901 tctactcata tagggacgca ttaacaaaga tatttgttat tgaggactca cctgcatgga
     3961 atccgaattt aaggtctaaa gtagctatca ggacaactga tacaaggtat tttgttgatc
     4021 cttcaatagc tgctgctgca ttggaactgg gacctgttga tttattgaac gatttaagta
     4081 ccatggggct tatttttgaa aacttatgtg tccgcgattt acggatatat gcagattact
     4141 tggatggcag catttatcag tacagagata gcaacggact ggaatgcgat gcggttattc
     4201 acttaagaaa cggctcttac ggtctcattg aaataaagtt gggcggtgac agtttgattg
     4261 aggaaggtgt caataaccta aacaaattgg cttca
//
LOCUS       DAOOJC010000197         4293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16053_length_4293_cov_3.585890, whole genome shotgun sequence.
ACCESSION   DAOOJC010000197 DAOOJC010000000
VERSION     DAOOJC010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16053_length_4293_cov_3.585890"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3361
                     /locus_tag="PLA01_06270"
     CDS             <1..3361
                     /locus_tag="PLA01_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPD00939.1"
                     /translation="IEWDDVWSTGGRIDYKLYISENSGFANTPPIYIKRGQIGENGPV
                     KVNEESGKLEYIHTARDPGRVYYIRIEPDVADSELKKSQYSRTVVVSSFILVKTTKVS
                     TDDSGAVWKLEWSPVVTGLGESEIKITYHIYRGEVDSSALPQYMAAVDGTSFFVTLPS
                     GNVENYFIIRALVTRDGQDVYEGIRIESEQIIVTEQEVAFYPAVPEIVDEFTDSGGDI
                     IVSYEEMLEPHSATVMWRVPLIGDGQIDEDVVYDIWLIQDPDQIDNPPQESLIASDIK
                     IGSNDYILNYNNILGYSYTVSNLTANSTYYFKIVAKRQFLEYVDDILQNVIYSSDPAI
                     KVIVTPPEGPIDQPNVPARPPLKVKEYSDGSDSVTQDSVIIQLKNMWYEKYNFEEDRW
                     EYIRTEKLHEADVPPYVPVSEPGPDPEPDTAPIPIVVDDVYYRKVSYDAGVKIEVGCI
                     EYIEGMTYDGLKDIAANWVNGFSVEPNDPLENPQLNPDKNKHNIDIPLAGLEPNTAYV
                     IWVRASRPELNLVSEPSDPIIITTDPVIIPPAEKPVVPSFNYSLSGDIYIDLGWEFKP
                     GYSYYIRYGIEDNINSAIGSVKVGMEDLYNSMYYRVGGLKPGTLYYFWLQAESSSGGE
                     AIKSEWSDSYLVSTLALIPPDSPKGFGIKNSSDAVTKNSITFEWIQEENLKYILEISK
                     ALDFKESIEYDAGYVSEYTVNGLLSNHRYFARLYAYDPVKNMKSGSTHIISVRTIGSA
                     DDYDSDVDIEDVITGEYVDKAETVEKGEWNVKIVGVNADRFIEQVIKDNKLDVTIDLG
                     NPPAAYDKLRILASDKVFKSLTTLSENLTIKTEKISLVIRPGTITTESFNPFANRASG
                     IDYEICISYPDKVGSGVKNMAFKTEVARIEISVVNGGNLLPVKSLLKPLMVLAEYDDE
                     YSEGKTSGFMYDKGASAWNELDTAYIYNYDTGRGTVVYETLKTGDMVVAETGYDYYDD
                     IYYHNYESAINKVASLYRLKSIPGRLFEPDNYATLGSTVKIMFDVLEYEYGSDFMRQA
                     ARAGLIGPENVDGADRNCTVGEAYKMLIRLFELKTGSTLDARSKSEFFEINGLELTRD
                     NRKTVPVNEPIKRGEVLALVEKILVYIGELE"
     gene            3625..4272
                     /locus_tag="PLA01_06275"
     CDS             3625..4272
                     /locus_tag="PLA01_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPD00940.1"
                     /translation="MHIGYETRDGAKNEAYLDYDMSGAVPPIRIKLTNINDRDWVPFN
                     DIKGIEQEEYIIHLHDAGIVNGMPDGSFKPDSKITRAEFMVLMVKALNIEGTAENVQG
                     FTDIEGHWAKDIILIASKYGLVSGYGDRTMRPDNPISLAEVSSVISRAFEFKTAKNGI
                     YQKLKKGKWYSSSVKKMFDVGILSVNDGIYEDFSEEGLINRADCSMMMSRALSTY"
BASE COUNT         1344 a          799 c         1075 g         1075 t
ORIGIN      
        1 aatagaatgg gatgatgtat ggagcaccgg aggaagaata gactacaagc tatatatatc
       61 cgaaaacagc ggctttgcca atactccccc gatttacatt aaaagggggc agatagggga
      121 aaacggccct gtaaaagtga atgaagaatc gggaaagctt gaatatatcc atacagctag
      181 agacccggga agggtttatt atatcaggat agagccggat gttgcggaca gtgagttaaa
      241 aaagagccag tatagcagaa ccgtggtggt aagcagcttc atcctggtga aaacgaccaa
      301 ggtttccacc gacgattccg gtgcggtctg gaagctggag tggagtccgg ttgtgacagg
      361 attgggagaa agtgaaatta agattaccta ccacatttac aggggtgaag tggactcttc
      421 agccctgccc cagtatatgg cggcagtgga cggtacaagc ttctttgtta cgcttccttc
      481 cggtaatgtc gaaaactatt ttatcataag agccctggtg acaagagacg gacaggatgt
      541 gtatgaggga atcagaattg aatcggagca gattattgta acggaacagg aagtggcctt
      601 ctacccggcg gtaccggaga ttgtggatga atttaccgat tctggcggag acattattgt
      661 aagctatgaa gaaatgcttg aaccccatag tgccacggtt atgtggaggg taccgttgat
      721 tggggacggg cagattgatg aagatgttgt atatgacata tggctgatac aagacccgga
      781 tcaaattgac aatccgcctc aggagtcatt gattgcctcg gatatcaaaa tcggaagtaa
      841 tgattatata cttaattata ataatatatt gggttatagc tatacggttt ccaaccttac
      901 ggccaattct acatactact ttaaaatagt tgcaaagagg caatttttgg aatatgttga
      961 tgatattctt cagaacgtca tttacagttc ggaccctgcg atcaaggtta ttgttacacc
     1021 gccggagggt cccattgacc agcccaatgt gcctgcaaga ccgcctctta aggtgaaaga
     1081 gtacagtgac gggagtgact ccgttactca ggactctgtt atcattcagc ttaaaaacat
     1141 gtggtatgaa aagtataact tcgaagaaga ccggtgggaa tacattagaa ccgaaaaact
     1201 ccacgaggcg gatgtgcctc cttatgttcc cgtatctgag cccggtcctg acccggagcc
     1261 ggatacggct cccatcccta tagtggttga tgacgtttac tacaggaagg tgtcctatga
     1321 cgccggtgta aaaatagaag tcgggtgtat agaatacatc gagggaatga cctatgacgg
     1381 gcttaaagac attgccgcca actgggtaaa cggtttttcc gttgagccca atgacccgtt
     1441 ggaaaaccct cagctcaatc ccgataaaaa caagcacaac atcgatattc cgctggcagg
     1501 tttagagcct aatacagcat acgttatctg ggtgagggcg tcaagaccgg aattgaacct
     1561 tgtatccgag ccttcggatc caataataat caccactgat cccgttatta tacctcccgc
     1621 ggaaaagccg gtagttccgt ctttcaacta cagtctttcg ggggatatat acatagattt
     1681 aggctgggag ttcaagccgg ggtacagcta ttatataaga tacggaattg aagacaatat
     1741 caattcagcc ataggaagtg tcaaggtcgg gatggaagac ctttacaact ccatgtatta
     1801 cagggttggg ggtttaaaac ccggtactct ttactatttc tggctgcagg ctgagtcctc
     1861 aagcgggggc gaagccataa aatctgaatg gagcgattcc tatcttgtaa gtactttagc
     1921 ccttatcccg ccggacagcc ccaagggatt cggtataaaa aacagcagcg atgccgttac
     1981 aaaaaatagc attacctttg aatggataca ggaggaaaac cttaagtata tccttgagat
     2041 atcaaaggcc ttggacttta aggaatctat agaatacgat gcgggatacg tttcggagta
     2101 tactgtgaac ggattattgt caaaccaccg ctactttgca agactctatg cctatgaccc
     2161 tgtgaagaat atgaaatccg gctcaaccca cattatatcg gtgaggacta taggcagtgc
     2221 tgatgattat gattcggatg ttgacattga agacgtaata accggtgaat atgtggataa
     2281 ggcagaaacc gtagagaagg gagagtggaa cgttaaaata gtgggtgtga acgccgacag
     2341 gtttatagaa caggtcataa aggacaataa actggatgta accatagacc tcggcaatcc
     2401 gccggcagcc tatgacaaat tgcggatact ggcttccgac aaggtgttta aatccttaac
     2461 caccctttcg gaaaacctga ccataaaaac ggaaaagatc tcccttgtta taaggccggg
     2521 aacgataacc actgaaagtt tcaatccctt tgccaataga gcctccggga tagactatga
     2581 gatatgcatt tcctatcctg ataaagtggg ttcgggtgtt aagaacatgg cgtttaagac
     2641 cgaagtggca cggattgaaa taagcgtggt taacggtgga aatcttttac cggtaaaatc
     2701 cctcttaaaa cctcttatgg tattggcgga atatgatgat gaatacagtg aaggcaagac
     2761 atcgggattt atgtacgaca agggagcctc agcatggaat gagcttgata cggcgtatat
     2821 atacaattac gacaccggca gggggactgt tgtctatgaa actctgaaga ccggggacat
     2881 ggttgtagcc gaaacgggct atgactatta tgacgatata tactaccaca attatgagag
     2941 tgcgattaat aaagtcgctt ccttataccg tttaaaaagt ataccgggaa gactttttga
     3001 accggataac tatgcaaccc ttggaagcac ggtgaaaata atgtttgatg ttttagaata
     3061 tgaatacggc agcgatttca tgaggcaagc ggcaagagcc ggactgatag gccctgagaa
     3121 tgttgacgga gcagacagaa attgtactgt gggcgaagca tataaaatgt tgataaggct
     3181 gtttgagctt aagaccggaa gcactcttga tgcccggagc aaatccgaat tcttcgaaat
     3241 aaacggtttg gagcttacaa gggacaatag aaagaccgtg ccggtcaatg aacccataaa
     3301 aagaggagag gtattggcac tcgttgaaaa gattctggta tatattggag aattggaata
     3361 aatatctgga ttatttttta aaatttagtc ctcaaaaaag aggatttggg gtgtatgaca
     3421 atgaatataa acaatataaa agtatgcata ataaccatag tgctgatttt tttcattaac
     3481 atgcaggatg cttgtgccca tggtgtatta tacgaactta agcaaacaag ttagaaaaaa
     3541 gtaagggtta cattaaagct ttcggaggaa gatcaaaaca gaggtattgc tatcacacac
     3601 cattatctgg aaaacgggaa agtattgcat attggctatg aaaccaggga tggagcaaaa
     3661 aatgaggcgt atctggacta cgacatgtcc ggagcagttc ctcccatcag aataaagctt
     3721 acgaacatta acgacaggga ctgggtacct ttcaatgaca ttaaggggat agagcaagag
     3781 gagtacatta tccatcttca tgatgcaggg attgtgaatg gaatgccgga cggttccttt
     3841 aaacccgaca gtaaaattac cagagcggaa tttatggttt tgatggtaaa agcattaaat
     3901 attgaaggaa ctgcagagaa cgtgcaaggt tttaccgata ttgaaggaca ctgggcgaag
     3961 gatataattt taattgcatc aaaatacgga ttggtttcag gttatgggga taggacaatg
     4021 agacccgata accccatcag cttggcggaa gtcagttcgg ttatttcacg ggcatttgag
     4081 tttaaaactg ccaaaaacgg aatatatcaa aagctcaaga agggcaaatg gtacagcagt
     4141 tcggtcaaga agatgtttga tgtcggaatt ctatcggtaa acgatggcat atacgaagat
     4201 ttcagcgagg aagggttgat aaacagggca gattgctcga tgatgatgag ccgggctctt
     4261 tcaacgtatt aataacgaga gtttatagcc tga
//
LOCUS       DAOOJC010000198         4276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16131_length_4276_cov_3.271737, whole genome shotgun sequence.
ACCESSION   DAOOJC010000198 DAOOJC010000000
VERSION     DAOOJC010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16131_length_4276_cov_3.271737"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLA01_06280"
     CDS             complement(<1..695)
                     /locus_tag="PLA01_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00941.1"
                     /translation="MPIKLKAYVAIIILLGTAFFVFTLNQAYEVDVVGLIVFTLLSII
                     AESLVIVTRGQRALSVGFVIALAAIMLFGVPEAAWITSLGIMLRIIRHEGKIFHPFNF
                     PIEKTLFNGANIALSAGLAGLCYEYLGGTPGVIDLNSSIAAIFAAITAMIVYILVNAA
                     IMSILMHLITGENFFSNFLNNIAWVIKDYIAMAPLAILMAIAYISYKIAGILIIFGPL
                     LFARYSFKMYIDM"
     assembly_gap    752..761
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(917..1549)
                     /locus_tag="PLA01_06285"
     CDS             complement(917..1549)
                     /locus_tag="PLA01_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00942.1"
                     /translation="MSNNKTILQNDIQSLICKLPEIINCSVICDKEGTIEEIHVLASL
                     GRNIKQMVRDIQSAISAKFNISVDYKKISIAQINEAEFKETRVRIEGIQVKNVDNAIE
                     AEVTLSYDDKVYKGRSSKVKSKSNRVKAVAEATLAALEACIGISGVLYLEGLESLKVS
                     DKEVYVSLVGFSKDNVDEYYSGSSIISLDENESAVKAVLSAVNRKVNTVA"
     gene            complement(1775..2305)
                     /gene="raiA"
                     /locus_tag="PLA01_06290"
     CDS             complement(1775..2305)
                     /gene="raiA"
                     /locus_tag="PLA01_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003491944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPD00943.1"
                     /translation="MRTTVSGKNIDITNALKERVEKKLSKFERYFGPDTEAHATLSVE
                     KNRHIIEVTIPFNGVILRGEEATGDMYTSIDNVVEKLERQMRRQKTKLEKRIKDGNIR
                     FENWAVPSSELEDEEVKIVRTKRFAMKPMPVEEAVLQMELLGHSFFVFSNADTEEVNV
                     VYKRKDGNYGLIEPEF"
     gene            complement(2837..3124)
                     /locus_tag="PLA01_06295"
     CDS             complement(2837..3124)
                     /locus_tag="PLA01_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HPD00944.1"
                     /translation="MFEDKTLICKDCGNEFTFTAGEQEFYAEKGFQNEPARCKDCRMA
                     RKNASGRNGQKREVVMYDTVCADCGKETQVPFKPTNARPVYCKECFDAKRQ"
     gene            complement(3539..4015)
                     /gene="flgN"
                     /locus_tag="PLA01_06300"
     CDS             complement(3539..4015)
                     /gene="flgN"
                     /locus_tag="PLA01_06300"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HPD00945.1"
                     /translation="MVDNTIVELMSLLEKKKKLFDSIMEITLEQKKELDNNETDKMQS
                     LVGRKQSVIDSIDEIDRSFSQGINTLKKHLNVQALEEVDFTKYPELKGLKLKVEEIMS
                     KAQEIMLIEMSNKEKLMSIMNGMKKEINQIKAGKKSIKAYEKPIINNDGIYIDKKK"
     gene            complement(4090..>4276)
                     /locus_tag="PLA01_06305"
     CDS             complement(4090..>4276)
                     /locus_tag="PLA01_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HPD00946.1"
                     /translation="DMQYEISEELFSLYEYMNRRLLEANIKKDKQILEEVTGYAEELR
                     DTWAQAMKLAKQQDAVR"
BASE COUNT         1179 a          972 c          664 g         1451 t
ORIGIN      
        1 ctcatatcaa tatacatttt gaaggaatat cttgcaaaaa gcagcggccc gaagattatc
       61 aaaatacctg caatcttata gctaatataa gctattgcca taagaatagc taaaggagcc
      121 attgcaatgt aatccttaat tacccatgca atattattta ggaaattgga aaaaaagttt
      181 tcgccggtga taagatgcat aagaattgac attattgcgg cattaacaag tatatatact
      241 atcatcgctg ttatagccgc aaatatcgcg gctatcgatg agttcagatc tattactcct
      301 ggagttcccc ccaagtactc atagcacaat cctgccaagc cagctgataa tgcaatatta
      361 gccccgttaa ataatgtctt ttctattgga aagttaaagg gatgaaatat ttttccttca
      421 tgccttatta ttctaagcat tattcccaaa ctagttatcc aggcagcttc aggcacacca
      481 aatagcatta ttgcagcaag tgcaattaca aacccaacag acagcgccct ctgcccccgg
      541 gtaactataa ccagtgattc agcaataata gataataggg taaaaacaat cagacccacc
      601 acatcaacct cgtaggcctg atttaatgta aatacaaaaa atgctgttcc taataatatt
      661 ataattgcta cgtatgcttt aagcttgata ggcatctatt cacctcataa aatggccggt
      721 atatgttata ctacccttaa aaccagctct gnnnnnnnnn ngctcctccg gctaatacca
      781 atgtgaaaat agccataaga ataccaagta tctttttcat tgtttgccct cctttaatca
      841 aggctctgcc tcaccgctat tcaagacagg caaactctcc gcttaaataa tataccggcc
      901 atattttacc ctttgtttat gctaccgtgt tcacttttct gtttactgct gatagtacag
      961 ctttaactgc agactcgttc tcgtcgagac tgattatgct gcttcctgaa tagtattcat
     1021 caacattgtc tttagaaaaa cccactaaag acacatatac ctccttgtca gagactttaa
     1081 ggctttccag cccctccaaa tacaacactc cgcttattcc gatacatgcc tccaaggctg
     1141 ccagtgttgc ttcagcaact gccttgaccc tgttggactt ggatttgact ttgctgctcc
     1201 ttcctttata aactttgtca tcgtaggaaa gcgtcacttc agcttctatt gcattgtcca
     1261 cgttcttaac ctgaatacct tcaatcctga ctcttgtttc cttgaactcg gcctcattga
     1321 tctgggcaat gcttattttt ttataatcca cagatatatt gaatttcgca cttattgccg
     1381 attgtatatc ccttaccatc tgcttgatat tcctgcctaa cgaagccaaa acatggatct
     1441 cttcaattgt gccttcctta tcacatatga cgctgcagtt tattatttca ggaagcttgc
     1501 atataagtga ttggatatca ttttgcagaa tggttttatt attgctcaaa gggaactcct
     1561 cctaaagtta tttgtgccgc gggaaacatt ggacatgcga tataatagtt tctataaata
     1621 cttttttatt ccttcctctt tatacaaatt agtcaaattg cactatcgaa tgggcaattt
     1681 gaactataca tataataact gctacaaaat aaataccata cctccgtgca ttagttccta
     1741 aaataaaaaa cagccgattt actcggctgt ttcatcagaa ttccggttca atcaaaccgt
     1801 agtttccgtc ctttctctta tatacaacat tgacttcttc cgtatctgca ttggaaaaca
     1861 caaagaagct gtgtcccagc aactccattt gcagcactgc ctcttcaacg ggcataggtt
     1921 tcatggcaaa tctttttgtc cttacaattt ttacttcctc atcctcaagc tctgacgaag
     1981 ggacggccca gttctcgaat ctgatgttgc catctttgat tctcttttcc agctttgtct
     2041 tttggcgtct catctgcctt tccaattttt ctacaacgtt atctatagaa gtatacatat
     2101 ctccggtagc ctcttctcct ctcaggataa ctccattaaa aggaattgtg acctcaatga
     2161 tgtgcctgtt cttttcaacg cttagagtag catgggcttc agtatcaggg ccgaaatatc
     2221 tttcaaattt ggaaagcttc ttttctaccc tttccttaag agcattggtg atatcaatgt
     2281 tttttccgct gactgtagta cgcataaaca catctccttt cgcaactatt atgctaccgt
     2341 ttatacatat tttattctac atatgagaaa ataatccttc tcattttcac aactttcaaa
     2401 caattatatt cagcggccgg aaagagcaaa attacagcat tttagccgca aaagcaagca
     2461 taattgaaac cataaagtta tgtaaagcga acaagagtat aaattaaagg gaacctgccc
     2521 tcgcccctca tatttcttag ttaatcacat tatgcacaaa gttatgcaca tcttttttat
     2581 gttatctgtg cataatgttg ataagtctgt tgataacttg aatctattaa ctttctattg
     2641 ttgaaaaaat gtgaattgct ttgtgggtga aaaatgtcga gcgaacaaag tgtaaaatat
     2701 tattttgatt tatcttgtcg gttggtggat atcaaaatcc ctgtgcaagt gctttgctgt
     2761 cgtaaaatgc tgtataatgt gcgggacaag ttatgcacag agggtataaa aaaaccgcct
     2821 tgataggcgg ttttaattac tgtctttttg cgtcaaagca ctctttgcaa tataccggtc
     2881 ttgcatttgt aggtttgaat ggaacctggg tttctttgcc gcagtctgca catactgtat
     2941 catacattac gacttctctt ttctgtccat ttcttccgct ggcattcttt ctggccatcc
     3001 tgcagtcttt gcatcttgcc ggttcgttct ggaaaccctt ctctgcatag aattcctgtt
     3061 ctcctgctgt gaatgtgaac tcatttccgc agtctttgca gattaacgtt ttgtcttcga
     3121 acatgtaaaa atccctctct tttcttctcc aggttggtgt tgggtaaaaa gaactttctg
     3181 ggatcattac tgccgcgaaa aacgttcttg aaccgcccac tcataccttt aaatatttat
     3241 ggcaatactc tagctgacct ggtctttgac cccacactcg ccgtcaggat gtttcgctac
     3301 taggaatcaa cccctcacta cagcacctct caataattaa ctattggacg gacttatttc
     3361 taagccgcac catgctcaca gaagcttctt ctgcttacgt ggatcgtttt gcctgcgact
     3421 ggcatcgatt ttcaaccaca gacctagata ttacaccaca ttctatatta tataatggaa
     3481 ttcttgattt aacaagtact tttgaaaaca attaggaatt aaatatcaga aaataccgtc
     3541 atttcttttt atctatatat attccatcat tgtttataat tggtttttca tatgccttta
     3601 tggatttctt cccggcctta atctgattta tctctttttt cattccattc attatggaca
     3661 taagcttctc cttgttggac atttctatga gcattatctc ctgtgccttt gacattattt
     3721 cttcaacctt aagctttaag cctttcaatt ccggatattt tgtaaaatca acctcttcca
     3781 atgcctgaac atttaagtgt ttcttcagtg tattaatccc ttgggaaaaa gacctgtcga
     3841 tttcatcgat actgtctata acagattgct tccgaccgac aaggctctgc attttatctg
     3901 tctcattatt atccagttcc tttttctgct ccagagttat ttccattatg ctgtcaaaca
     3961 gcttcttctt tttctcaagt aaactcatga gctcaacaat agtattatca accatctcaa
     4021 taaatcctcc aaaaaatcat agggattggc atccacgcag gaatgcctat ccctattatt
     4081 atataatatt taacgaacag catcctgctg ctttgccagc ttcattgcct gagcccatgt
     4141 atctctcagc tcttcagcat agcctgttac ttcttctaaa atctgtttgt ctttttttat
     4201 atttgcttcc agcagtctcc tgttcatata ctcgtacaat gaaaacagtt cctctgaaat
     4261 ttcatattgc atatcc
//
LOCUS       DAOOJC010000199         4250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16256_length_4250_cov_2.751609, whole genome shotgun sequence.
ACCESSION   DAOOJC010000199 DAOOJC010000000
VERSION     DAOOJC010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16256_length_4250_cov_2.751609"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PLA01_06310"
     CDS             complement(<1..554)
                     /locus_tag="PLA01_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HPD00947.1"
                     /translation="MLSKVKSIGLMGINGYTVMVETDISSGLPAFDMVGLGDTAVKES
                     KERVRAAIKNAGLDFPIKRITVNLAPADKRKEGSAFDLPIALGILSATGQINGSNIEK
                     YAFLGELSLDGGIKPVKGILPMVISAREDGIERIVLPVENADEAAVVKGISIFPAANI
                     IDVVNHLNGESEIKRHEVDVDAFF"
     gene            956..1375
                     /locus_tag="PLA01_06315"
     CDS             956..1375
                     /locus_tag="PLA01_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00948.1"
                     /translation="MLLFTRNRKKRNKPAMEEKPQKFDCAIEPSATNRPSSWDSVGFS
                     AHISNHIGETVTLFVESGGMSGTGFTGVLLYANEVYVKLLVSIGPAPFCPLGNPCILS
                     FSHSIKRLNPCVNPMRSMGAIAIIPVNRIVSFVHNGI"
     gene            complement(1459..1899)
                     /locus_tag="PLA01_06320"
     CDS             complement(1459..1899)
                     /locus_tag="PLA01_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00949.1"
                     /translation="MANLGAFSGGSFSALLSDYIGETVTIFTKSGGQSGAGFTGVILA
                     VNDCIVRLLTRIGPPPGCALGNACTGFNVGHGYGRGYMPSSGGCGVADGYSGGYGYGT
                     GQILPASENGAITAGGWNGYPVYTVGSVTDIPIDSIASFVHNAI"
     gene            complement(2013..3866)
                     /locus_tag="PLA01_06325"
     CDS             complement(2013..3866)
                     /locus_tag="PLA01_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HPD00950.1"
                     /translation="MNEVLEKNLVLLKKHQPGVYYKLERHIKGEYEPLDTSVEKILLA
                     RQNDLVINVMVRCGQKNYVLCDHEDPINEAYAWIDKYIDASNRADIVFGMGMAFHLEV
                     LLTSFPNKKVVVVEPNIDLFYQIICIRNLEPVIKKAEILVDESMGNILGRINSLLWDT
                     EEGGIQVEPFEVYAEMFPDTWDELRSRFIKHAESFTVDIATRRYFGDLWVHNNIKNLN
                     RIRGASNADGLIGKFKGIPGILVSAGPSLQKNAHLLKGLEDKCVIMAAGTAVRILEDF
                     GIAPHFMVGIDAGDKEGEIHANVKNKDICFIYSNQVSTLSVDSYDGPKFVMNYPVDIY
                     TAKFFEYSNIKSGFFLSGPSVANTCFDILFKMGCNPIIIVGQDLAFTSGSRYAGEAPG
                     TVIGDSDDSKSREYVSVKDIYGNDVYTTRAFLAMRNWFEGYFEKAIAHTEIINATEGG
                     INISHAVNDTLENALKSCNLSSCGIAGRIGAVYRENIFADSIVSKIDEYKTYIRKEIE
                     KLEQISSHQLELVDYLHKDIYHPSKNSSKFVKVIDSISNLSNKVMESPIYPSLLKNLI
                     EIDFFLIKSEVDRANKVLTKYEDIKNVYINAILSQNQKLTGSLNKIKEFLE"
     gene            complement(3904..>4250)
                     /gene="nrdR"
                     /locus_tag="PLA01_06330"
     CDS             complement(3904..>4250)
                     /gene="nrdR"
                     /locus_tag="PLA01_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPD00951.1"
                     /translation="FTTYEKVESLPLMVIKKDKSRQPYNREKLLNGLLRACEKRPVSI
                     DDLEKLVEDVESHIYNSLQREVTSKVIGELVMAKLKNLDEVAYVRFASVYRQFKDINT
                     FVDELHKLLKEK"
BASE COUNT         1242 a          994 c          707 g         1307 t
ORIGIN      
        1 ttgaaaaatg catcgacatc aacttcatgc ctcttaattt cggattctcc gtttaagtgg
       61 ttcacaacat ctatgatatt tgctgcagga aatatgctta tgccctttac aaccgcagct
      121 tcatcagcat tctctaccgg cagcactatt ctttcaatcc cgtcttccct tgcacttatg
      181 accatgggaa gtatgccctt tacaggtttt atccccccat ccagagacaa ctcccctaaa
      241 aacgcatact tttcaatatt gctgccgttt atctgcccgg ttgccgataa tatgcccaaa
      301 gcaatcggca aatcaaaggc agacccttcc tttcttttgt cggcaggagc aagatttacg
      361 gtaatcctct taatagggaa atctaaccct gcatttttta tagctgccct gacccgctcc
      421 ttggactcct ttaccgccgt atctccgagt cctaccatgt caaaagcagg cagtccgctg
      481 cttatgtcgg tttccaccat tacagtataa ccgtttatac ccataagtcc aatgctcttt
      541 accttcgata gcacaatttg ccacaacctt cctcaacatg tacagaataa taggtaataa
      601 attattctta cagtgtaaat aatttattac caacatattt ctacataaat gtgccatatc
      661 ctctatatat tcaatatgct ttgcctaaat tattttaaat cgctatgttt ctttcaccct
      721 ttttctcttt gcaccctaaa caaaagagca cttaaagcaa atgtaattaa aaatctgctc
      781 cgtttcaata aaaacagtaa catttacgga ctttatagct atgatatatt atagcaatgt
      841 cttgccgcta ttataacgaa gcaatagatg tcccgcactt ctttaaagct gcggcaaatg
      901 caataaatat caagaaatat atctcctttt aataaaatta cgggaggaat gttaaatgct
      961 tttattcaca aggaaccgta agaagagaaa taaacccgca atggaagaaa aaccgcagaa
     1021 atttgattgt gcaatagaac cttcagccac aaacagacct tcctcatggg acagtgttgg
     1081 cttttccgcc catatatcaa accacatagg tgaaaccgta accctgttcg ttgagtcggg
     1141 aggcatgtca ggaacaggtt ttaccggtgt actgttatac gccaatgaag tttacgtaaa
     1201 actgctggtc tccattggtc ctgccccgtt ttgtcccttg ggtaaccctt gcatccttag
     1261 tttttcacac agtattaaaa ggcttaatcc ctgtgtaaat ccgatgagaa gtatgggagc
     1321 aattgccata ataccggtta atagaattgt ttcctttgtc cataacggca tttaaatccg
     1381 ggtgtttcca gcgagtacat aaaaaaccgg gaaggttccc ggttttttta tcacaaatca
     1441 atgggaattc aacttatgct aaatagcatt atggacaaag gatgctatgc tgtctatcgg
     1501 tatatctgtt accgacccta ccgtatatac cgggtagccg ttccatccgc cggctgttat
     1561 tgcaccgttc tcacttgccg gaagtatttg accggttcca taaccataac ctccggaata
     1621 tccatcggca acaccgcatc ctccactaga gggcatatag cctcttccgt atccatgacc
     1681 aacgttaaat ccggtacatg cattacctaa agcacagccc ggcggtggac cgattctggt
     1741 tagcaatctg acaatacaat cattaactgc aagaataacg ccggtaaaac ctgctcctga
     1801 ttgaccgcca ctttttgtaa atatcgtaac agtttcacca atatagtccg aaagcaatgc
     1861 tgaaaagctt cctcctgaaa aagctcccaa gtttgccatt acaactttcc cctttcgctt
     1921 cttatacttt tctttcctta tatatcttat gtcaactgta atccgaatgt tacaggatgg
     1981 ataatattta agtaaagaaa aatatggagg aattattcta aaaactcttt tattttgtta
     2041 aggcttccgg taagcttctg attctgactt agtatggcat ttatatatac attctttata
     2101 tcctcgtatt ttgtcaatac cttgttagcc ctgtctactt ctgactttat aaggaaaaaa
     2161 tcaatctcaa tcaaattttt cagaagagaa ggataaatgg gagactccat caccttattg
     2221 gacaaattac ttatagagtc aataaccttg acaaactttg agctgttttt ggacggatga
     2281 tatatgtctt tatgaaggta atccaccagt tccaattgat ggctggaaat ctgttcaagc
     2341 ttctcaattt ctttgcggat atatgttttg tattcatcga ttttggaaac gatgctgtca
     2401 gcaaaaatgt tctccctgta cactgccccg attcggccgg caatgccaca agaactcaaa
     2461 ttacagcttt taagggcatt ttcaagagta tcgttaacag catgggaaat gtttattccc
     2521 ccttcagttg cgtttattat ttcagtatga gcgatagcct tttcaaaata gccttcaaac
     2581 cagtttctca tcgcaagaaa agcccgggtg gtatagacat cattcccgta aatatccttt
     2641 acagatacat actcacggct ttttgagtca tcagaatcgc ctataaccgt tcccggtgct
     2701 tcaccggcat acctgcttcc ggaggtaaaa gccagatcct gcccaacaat gataatggga
     2761 ttacagccca ttttgaacag aatatcaaag catgtattgg caacagacgg tccgctgagg
     2821 aaaaagccgg atttgatgtt cgagtattca aaaaacttag cggtataaat atcaacagga
     2881 tagttcataa caaatttagg tccgtcatag ctgtcgacag aaagtgttga aacctggttt
     2941 gaatagataa aacagatatc cttgtttttt acattggcat gtatctcgcc ttctttatcc
     3001 cccgcatcta tacccaccat aaaatgcgga gcaataccaa agtcctctaa aatccttact
     3061 gcggttccag ccgccattat aacacatttg tcctcaagcc cttttaagag atgagcattc
     3121 ttttgaagtg acggtcctgc agataccaga ataccgggaa tacccttaaa ctttccgata
     3181 agaccgtcag cattggaggc cccccttatt cggttgagat tctttatgtt gttatgaacc
     3241 cagagatctc caaaatacct tctggttgca atatccaccg taaaactctc ggcatgctta
     3301 ataaacctgc ttctaagctc gtcccatgta tcgggaaaca tctctgcata tacttcgaaa
     3361 ggttctacct gaatacctcc ctcttcggta tcccacagca atgagtttat cctgccgaga
     3421 atgttgccca tactttcatc aacaaggatt tctgcttttt taatcaccgg ttcaagattt
     3481 cttatacaga taatttgata aaacaaatca atattaggct ctacaacaac cacttttttg
     3541 ttcggaaagc ttgtaagaag gacttccaaa tgaaaggcca tacccatacc gaagacgata
     3601 tccgccctgt ttgaagcatc aatgtatttg tcaatccaag catatgcctc gttaatgggg
     3661 tcctcatggt cacataaaac ataattcttc tgtccgcacc ttaccataac atttattaca
     3721 agatcattct gacgtgccaa aagaattttt tccaccgaag tgtctaaggg ttcgtactct
     3781 cctttaatat gccgttcaag cttataatat acccccggtt ggtgtttttt aagaagcacc
     3841 agattttttt ccaaaacctc attcatgcta tatcactcat ttcggaataa ttggattgaa
     3901 taactacttt tctttaagaa gcttatgcaa ctcatccaca aatgtattaa tatccttaaa
     3961 ctgcctgtat accgatgcaa accggacata agcaacttca tcaagatttt tcagcttagc
     4021 cataaccaat tctcctatga cctttgaggt tacttccctc tgcaatgaat tataaatatg
     4081 gctttcaaca tcctccacaa gcttctctaa atcatcaatg gaaacaggtc ttttttcaca
     4141 cgctcgtaaa agcccgttaa gaagcttttc cctgttatag ggctgtcttg acttatcctt
     4201 ttttataacc ataaggggca aactctcaac cttctcatag gtagtaaatc
//
LOCUS       DAOOJC010000200         4245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16276_length_4245_cov_3.057995, whole genome shotgun sequence.
ACCESSION   DAOOJC010000200 DAOOJC010000000
VERSION     DAOOJC010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16276_length_4245_cov_3.057995"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1284)
                     /locus_tag="PLA01_06335"
     CDS             complement(94..1284)
                     /locus_tag="PLA01_06335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD00952.1"
                     /translation="MNILFVNANLYGHINPTLGLVKKLTEKGNQVSYFCSESFSGQVT
                     KMGAKWIGYSNRLDLFLKEYRPTDRHPFFMLMEYMLLYDEAVLPEILGIIEEGQYDMI
                     ICDSYFGGACFLKQIVKIPVVCSHSSFAMSKAPVPERMLAPGFHPQLDHCYRIIKRIC
                     EGYNIEEPSLEQVFTSKGDLNIVYTTQDFNGDDGVHEPEYLFAGPSIDRQQEAEDLDL
                     SVIGDRKLIYISLGSVNTAYLDFYKTCISAFSNTDYYVCMSIGKKCEVSQLGEFPQNF
                     LVKGYLPQLEILKRADVFITHAGFNSVNEALYFGVPMLALPQVNDQHKVAQRLVSMGL
                     GMSEDIKELSPDILRLKTEELIMDRKIKGNCMTISREMRNLTEFEDTVGKLEKFVHTW
                     KERR"
     gene            complement(1326..1622)
                     /locus_tag="PLA01_06340"
     CDS             complement(1326..1622)
                     /locus_tag="PLA01_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00953.1"
                     /translation="MKTKFFSLSLMAYLVIGFLWLTSGVKFRFVVVLGAFMIAANLIC
                     ETLLSIINTVDIVDAVYGTVGIILAFIYSCCVNSNGLIQINLANIENILTTISE"
     gene            complement(1623..2060)
                     /locus_tag="PLA01_06345"
     CDS             complement(1623..2060)
                     /locus_tag="PLA01_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00954.1"
                     /translation="MLFRKQYGRIRRIRNTHPIILLPLVIVIIISISACSVPKGSIVI
                     LENPNGTGVTMDFKEWNSKSKCELSLNKGDVLQIEVDREYGEIALTVSGKNGSEPYTG
                     NNLKSGLFTVTVSETDKYDIRITGKDATGKVTVKNVENVGENH"
     gene            complement(2030..3235)
                     /locus_tag="PLA01_06350"
     CDS             complement(2030..3235)
                     /locus_tag="PLA01_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPD00955.1"
                     /translation="MIQTILFFILTVLAVFTFAVLLIRTAYCAIMYRMKKISLKHIIR
                     LRKTMIFFAVTMILNVGLITFSQLTASTPRIVDENGNTPPNSIAELRELELNGRKQWI
                     SLRGWDKNAPVLLFLAGGPGGTQMAAVRHELAELEKHFVVVNWDQPGSGKSYYAEKTK
                     NITAQTYIQDGHALTEYLKERFSQKKIYLVGESWGSALGIFLVSEYPESYHALIGTGQ
                     MIDFAETERMDYAKAMEIAQSKGDTALIKRLIANGEPPYYGDDVTWKSAVYLNYLSAY
                     MASNPKIHNSGYNTFRDIGSSEYGLLDKINFFRGIINTYNHVYQQLYTIDMRTDYTKL
                     DVPVYFFLGRYDVNAPTVLAEEYMQVLDAPDKKIVWFEHSGHSPWINEPEKFVKEVLS
                     CFSGSNTEE"
     gene            complement(3249..3800)
                     /locus_tag="PLA01_06355"
     CDS             complement(3249..3800)
                     /locus_tag="PLA01_06355"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD00956.1"
                     /translation="MKYKPTHLEIFLTRIVFFLCGKPVYRTFADRLPLDGGEQVLDFG
                     CGMGTVAYYAAKKLTHGHLTCLDISERWLNACRKTLRGYRNISFMLWESLQLTNESFD
                     VAYCHFVLHDISESELERVIPKLAGSLKPGGVLVFREPFNETNKISLIKRLIEQNRLI
                     SKDSRITDIPVMGNALECVYIKK"
     gene            complement(3994..>4245)
                     /locus_tag="PLA01_06360"
     CDS             complement(3994..>4245)
                     /locus_tag="PLA01_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD00957.1"
                     /translation="NSGTKSLVYDQDFSDDGIGIPDHLMSNIFKPFVRADDSRNSKTG
                     GSGLGLSIAKRIAEAHGGDLTLLPDKNKGSTFRITIPAI"
BASE COUNT         1210 a          998 c          751 g         1286 t
ORIGIN      
        1 tgtcactgta cttttttatg tcatagccgc tacttggtga cagattcaca atacccaaaa
       61 tagcatatat ggatttattc tttaatgcca ttcctatctc ctctccttcc aagtatgaac
      121 aaatttttca agcttcccta cagtgtcttc aaactcagtt aaattcctca tctcacgaga
      181 aattgtcatg caatttccct tgatttttct atccattata agttcttctg tctttaatct
      241 caatatatct ggtgacaatt cctttatgtc ttctgacatt ccaagcccca tagaaacaag
      301 acgctgggcc actttatgct ggtcattgac ctgaggtaac gcaagcatag gaactccaaa
      361 gtatagagct tcgttcacgc tgttaaaacc tgcatgagta ataaatacat cagcccgctt
      421 taaaatctcc agctgcggaa gataaccctt taccagaaaa ttctgtggaa attcccctaa
      481 ttgtgagact tcacacttct ttccgatgga catgcataca taataatccg tattactaaa
      541 tgccgagata catgtcttat aaaaatctaa ataagctgta ttcacacttc caagcgatat
      601 atatatgagc tttctgtctc ctatcacgga aagatctaaa tcttctgctt cctgttgccg
      661 gtcaatggac ggaccggcaa ataaatattc cggctcatgt accccatcat ctccgttaaa
      721 atcttgagtt gtgtatacaa tattcaaatc tcctttactt gtaaaaacct gttctaatga
      781 aggttcctca atattatagc cttcacaaat tcgttttatt atccgatagc aatgatccaa
      841 ttgaggatgg aagcctggtg caagcatcct ctccggcacc ggtgctttac tcatcgcaaa
      901 agaggaatgg gaacacacaa ccggaatttt aacaatctgt tttaaaaaac atgctcctcc
      961 gaaataagaa tcacaaataa tcatgtcata ctgaccttct tcaataatac ctaaaatctc
     1021 cggcagcact gcttcatcat ataaaagcat gtactccatc aacatgaaaa acggatgtct
     1081 gtctgtaggc cggtattctt tcagaaacag atccaacctg ttgctatatc caatccattt
     1141 tgcccccatc tttgttacct gtcccgaaaa ggattcagag caaaaatagc tgacctggtt
     1201 tcctttctct gtaagtttct taaccaatcc aagggtaggg ttaatatgcc catacaaatt
     1261 tgcatttaca aacaaaatgt tcattttctt ctccttatat cactctgata tagttacata
     1321 atatatcact ccgatatagt tgtcaatatg ttttcgatat tagctaaatt gatttgaatc
     1381 aaaccgttac tattaacgca acaggaatat ataaatgcca gaatgattcc aactgttcca
     1441 tatacggcat ctactatatc tacagtattt ataatgctca ataatgtctc acaaattaaa
     1501 ttagccgcaa tcataaatgc acccaaaaca actacaaaac gaaatttcac tccggatgtc
     1561 agccacaaaa aacctattac aagataagcc ataagactta atgaaaaaaa ttttgttttc
     1621 actcaatggt tttcgccaac gttctccaca tttttgaccg taacctttcc agttgcatct
     1681 ttaccggtaa ttcttatgtc atatttgtcc gtctcagata ctgttacagt gaataaacct
     1741 gacttaaggt tgtttcccgt ataaggttca ctgccgtttt tgccgctgac cgtaagagcg
     1801 atttctccgt attcacggtc aacttcaatc tgcagcacat cccccttgtt aagggataat
     1861 tcacacttgc tttttgagtt ccactcctta aaatccattg tgactcctgt tccattcgga
     1921 ttctcaagaa ttacaatgct gccctttggc acggagcaag ccgagatcga tataataatg
     1981 acaatcacca gaggcagaag aataatcggg tgcgtattcc gaatcctcct tattcttccg
     2041 tattgcttcc tgaaaagcat gacaatacct cctttacaaa tttttccggt tcgttgatcc
     2101 atggactatg accggaatgc tcaaaccata caattttttt atcgggtgca tccaaaacct
     2161 gcatatattc ctcggccagc actgtgggtg cattgacatc atatcggccc aggaagaaat
     2221 agacaggcac atcaagttta gtgtaatccg ttctcatatc gatggtataa agctgttggt
     2281 acacatggtt atatgtgttg atgatgccac ggaaaaagtt gattttgtca agcagtccat
     2341 attcggagga accgatgtcc cgaaaggtgt tgtagcccga attgtgaatt ttagggttgc
     2401 ttgccatata agcactgaga tagttcaggt acactgcact tttccatgtt acgtcatctc
     2461 cgtaataggg tggttcaccg tttgctataa gccgctttat gagagcagta tcgcctttgg
     2521 actgtgctat ttccatggcc tttgcgtaat ccatccgctc ggtttcggca aagtctatca
     2581 tttggcctgt gccgatcaat gcgtgatacg actccgggta ctcagagaca aggaaaatcc
     2641 ccaaagcact gccccatgat tcacccacca gatagatttt tttctgcgaa aagcgttcct
     2701 ttaaatactc cgtcaaagcg tgaccatcct gaatataggt ttgggctgta atgtttttcg
     2761 ttttctctgc ataataggac tttccggagc cgggctgatc ccagttgaca acgacaaaat
     2821 gcttctcaag ctccgcaagc tcatgccgca ccgctgccat ctgcgtaccg ccgggacctc
     2881 ctgccaggaa tagcaaaacg ggtgcgtttt tgtcccaacc tctcagactg atccactgct
     2941 tccttccgtt cagctcaagt tctctaagct ctgcaatgct gtttgggggt gtgtttccgt
     3001 tttcatcaac aatacgtgga gtggaagcgg taagctgaga aaaggtaatt aatcctacat
     3061 tcaatatcat tgtcaccgcg aagaatatca ttgtttttct caggcgaata atatgtttga
     3121 ggctgatctt tttcatcctg tacataatgg cacaataagc cgtcctaatc aagagtactg
     3181 caaacgtaaa gactgccaac acagttaata tgaaaaatag tattgtctga atcataagta
     3241 cacctccatt attttttgat atatacacat tccagggcat tccccatcac cggaatatcg
     3301 gtgatgcggc tgtccttgga aattagcctg ttttgctcta tcagacgttt gataaggcta
     3361 attttattcg tttcattaaa cggttcgcgg aaaaccaata caccaccggg tttaagagac
     3421 ccagcaagtt tagggatgac tctttccaat tcactttccg aaatatcgtg cagtacaaag
     3481 tggcagtacg ctacatcaaa gctttcattt gtgagctgca aagattccca taacataaaa
     3541 ctgatattcc tataaccgcg aagagttttg cggcaggcat tgagccatcg ctcggaaata
     3601 tccagacagg tcaaatgccc gtgggtcagc ttttttgcgg cgtagtaagc aaccgttccc
     3661 atgccgcacc cgaaatcaag cacctgctct ccgccatcaa ggggcagccg gtccgcgaat
     3721 gtccggtaga caggcttacc gcaaaggaaa aatacaatcc ttgtcagaaa aatctctaaa
     3781 tgggtcggtt tatacttcat tactcatcac ctcagcatat ccaatcatga ctctagtttt
     3841 tgcatttgct ttgttcttgt gtcataacaa ccccccttaa aataaaatca gctgtatttc
     3901 aggctcatta ttatcataac aatcgaacct gtaatagctg atatgaatct taaatttacc
     3961 ttaaataatc tgctccgttt tatataattt cctttaaatc gcaggaatcg taatccggaa
     4021 agtgctgccc ttattcttat cgggaagcag agtcaaatct cctccgtgag cctcggctat
     4081 tcttttcgct atagaaagcc ccaatccgct gccgccggtt ttggagttgc gggaatcatc
     4141 tgcccggaca aaaggcttga atatgttgct cataagatga tccggtatac cgatgccatc
     4201 gtcactaaaa tcctgatcgt aaactaaact ttttgtgcca gaatt
//
LOCUS       DAOOJC010000201         4242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16291_length_4242_cov_3.149272, whole genome shotgun sequence.
ACCESSION   DAOOJC010000201 DAOOJC010000000
VERSION     DAOOJC010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16291_length_4242_cov_3.149272"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..909
                     /locus_tag="PLA01_06365"
     CDS             274..909
                     /locus_tag="PLA01_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPD00958.1"
                     /translation="MVSILMVDDHKAVLCGTKMLLESHGMQVTTCHSGIDALEILKKH
                     SFDVMIYDLKMPDMNGLELTKKTLEIYPDATIIILSGENIAENFDILVNSGVSGIIDK
                     SCSDNQLITGIYMAMEKMMILPLDLVRKLAINKMSVNQSTEKLEEPLTEIELEILQHA
                     AAGKTNKEIADELQMVQRNVEYHLSHVYKKMDVPSRVYAIRKGIKLNLING"
     gene            complement(917..2911)
                     /locus_tag="PLA01_06370"
     CDS             complement(917..2911)
                     /locus_tag="PLA01_06370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HPD00959.1"
                     /translation="MKTEKTGLSCDSTVKRIEKAIILRLRTKMNLLFSAIIIIIGTTL
                     GYSIYVASIDLIVESIGIKAKNIVENVANNIDMDELQKVTERTLQIEEEKGDVQEILD
                     MPEYQKLRQQLWDFKKFYRMKFLYIMTKTKTGESIYVIDALPLDYKGNDVSLPGEVEF
                     NTYEYLEAAIDEGEAFIGELTKDRRWGANLASYVPLTISEGDTIGVLGIDIEAEDIYE
                     LMAQTRNSIGLYTAVAVIVVLFISWLTIGVVTRRLRKLTSKVQKVREGDYSVEFDTNE
                     RDEIGELEVAFDELVGVFNDSNIRINNSLIDLSKSHKVEELDQKIISNIKNALSIESV
                     DILEFDRENINSHKTKLNLPYPYIQKYLRENVKCLKIGNLVKINEGFLTLVGIVEDRY
                     RILFVDGQNVSLSQRDKLSIKLLSRYIAVFYENLTLIEELSNKIMDFQNGGDAPSWMS
                     KVFLQISEKERKRLASDLHDEILQSIIKMRRDVSNFLENRSVGKDDVLNQLDRMEEEF
                     DDVILMIRETCNELLPSFLSEKGIVRAIDRLIERVSLKTDIAINFEAFNVDSRLEYEE
                     VLTVYRVVQELINNAIAHSKGTEVDLMLRQDMEKFVIYYNDNGVGMDLNAKIDTNKHL
                     GLSGIKERIRLLNGSVTVNSELGKGLEFSCQFPTKKIIAC"
     gene            complement(2912..3679)
                     /locus_tag="PLA01_06375"
     CDS             complement(2912..3679)
                     /locus_tag="PLA01_06375"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPD00960.1"
                     /translation="MILVMDVGNTNTVLGVYDGKKLINHWRMSTDRGKTSDEMGMFLL
                     SLFNYEKIDIEKVEAVMIASVVPPIMYSIEHAIRKYFQKTPMVVGPGIKTGINIMYEN
                     PREVGADRIVNAVAAFEIYGGPVIIVDFGTATTFCAISSRCEYIGGVICPGMQISSEA
                     LFEKTAKLPRIDLSKPKTVIGRNTVSSMQSGMVYGYVGQVDYIVKRMKQEMKESNIKV
                     IATGGMARLIASESETIDETNGLLTLEGLRIIYERNK"
     gene            complement(3697..4188)
                     /locus_tag="PLA01_06380"
     CDS             complement(3697..4188)
                     /locus_tag="PLA01_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00961.1"
                     /translation="MVDNNNGNNGSMSNINGNFDGNDNNGGLNNRNKRKKGFYKKNGS
                     EAASKDNSRGDFSNKPGNILKGNRSNNKTGKQNKDGASTSRSSYSGKDTSHQRDNNFH
                     KGNSFKRDSGQSKNTGSGYSYRDSGRSKTEETVEDIKSDIVRIEKEIMLEIKEIRGLK
                     LGV"
BASE COUNT         1186 a         1000 c          660 g         1396 t
ORIGIN      
        1 tagtgaaatc cctccgataa tagccccagg ggtgccaaca accatcgttt cgggtttatc
       61 tccctttaca aataaaaata tagcatactt ttttctacgt gtcaccgaaa tatttccatt
      121 atattccgac aaaaacgagc acttgctttt ttcgttctct tttcggaatc catcactttt
      181 cttcggtgca gttacggtat tttttcggtg tccaacaacg aaatatatgc tataataggc
      241 atatcgaaaa cttttgttgg attgagggat ttaatggtaa gtatcttaat ggtagatgat
      301 cataaagctg ttttatgcgg cacaaaaatg ttattggaga gtcatggcat gcaagtaaca
      361 acatgtcatt ccggtataga cgccctggag atattgaaga agcattcttt cgatgtaatg
      421 atatacgatc tgaaaatgcc ggacatgaat ggactggagt taactaaaaa aacactggag
      481 atttaccccg atgccactat tattatactg tccggtgaga acatagctga aaactttgac
      541 atccttgtta attcgggtgt cagcggaata attgataaat cctgcagtga taatcaattg
      601 attactggca tatatatggc aatggaaaaa atgatgatac tccccctcga tctcgtcaga
      661 aaactagcca taaacaaaat gtcggtaaat cagagtacag aaaaattaga agaacccctg
      721 actgaaattg aattggaaat tcttcaacat gctgccgcgg gtaaaacaaa caaagaaata
      781 gccgatgaac ttcaaatggt gcagcgtaac gtcgaatacc atttgagcca tgtctataaa
      841 aagatggatg taccttcccg tgtttatgcc attcggaagg gtatcaagct taatctgata
      901 aatggctgaa atattatcag caggcaataa tctttttagt aggaaactga caggaaaact
      961 caagtccttt tcccagctcg gagtttacgg taacacttcc gttgagtaac cggattctct
     1021 ctttaatgcc gcttaaccct aaatgcttgt tagtatctat ttttgcattc aaatccattc
     1081 ctacgccatt gtcattgtaa tatataacaa atttctccat atcctgccga agcattaagt
     1141 ccacttcagt acccttcgaa tgagctatag cgttatttat caactcctga acaaccctgt
     1201 agaccgtcag cacctcttca tattccagcc ttgaatcaac attaaaagct tcaaaattaa
     1261 tagcaatatc agttttaagg gacacccttt caattaaccg gtcaatagcc ctgacgatac
     1321 ctttttcgga aaggaaggaa ggcagcagct cattacaggt ttcacgaatc atcaatatta
     1381 catcgtcaaa ctcctcttcc attctatcca attggttcaa aacatcatct ttgccaaccg
     1441 accgattctc aagaaagtta ctgacatccc ttctcatttt tattatgctt tgaagtatct
     1501 catcatgcaa atccgaagcc agtctttttc tttctttttc ggaaatttgc aagaatactt
     1561 tgctcatcca cgaaggagca tctcctccgt tctggaaatc cataatcttg ttggacaact
     1621 cttcaatcaa agtgagattc tcgtagaaaa ctgcaatata ccttgaaagc aatttaattg
     1681 acagcttatc cctttgactc aaacttacat tttgtccgtc aacaaacagt atcctgtacc
     1741 tatcctccac aattccaacc agagtcaaaa atccttcatt gattttaacc aaattgccaa
     1801 ttttaagaca ttttacattt tcccttaaat acttctgtat gtaggggtaa ggcaggttta
     1861 actttgtctt gtgcgagtta atgttttctc tgtcaaattc taaaatgtcc accgattcaa
     1921 tcgaaagagc attctttata ttgcttataa tcttttgatc cagctcctcc accttgtgtg
     1981 actttgagag atctataagg ctgttgttta ttcgaatatt gctgtcgtta aacacaccca
     2041 ccagttcatc aaaagctacc tccaactcgc cgatttcatc cctttcattt gtatcaaact
     2101 caaccgaata atctccttca cgcacttttt gtactttact tgtcaacttt ctcaagcgtc
     2161 gggtaactac tcctatagtc aaccacgata taaaaagaac aacaattacc gcaacagcag
     2221 tatataaacc tatactattt ctggtttgtg ccataagctc atatatatcc tctgcctcaa
     2281 tgtcgattcc gaggactcca atcgtgtcac cttcagagat tgttaacggc acatatgagg
     2341 caaggtttgc tccccaccgg cgatctttcg tgagttcacc aatgaaagct tccccttcgt
     2401 ctatcgcagc ttctaaatac tcatatgtat tgaattcaac ttctcccgga agcgaaacat
     2461 cattcccttt ataatccaga ggcaacgcat ctataacata tatgctttcc cctgtctttg
     2521 tttttgtcat aatataaaga aatttcatcc tataaaactt tttaaaatcc cataactgct
     2581 gtcttaactt ctggtattcc ggcatgtcaa gaatctcctg aacatcccct ttttcttcct
     2641 caatttgaag agtccgctcc gttaccttct gcagctcatc catgtcaata ttattcgcca
     2701 cattttctac tatattttta gcctttatac ctatagactc tacgattaaa tcaatagagg
     2761 caacataaat agaatatccc aaggttgttc cgataataat tattattgct gaaaacaata
     2821 gattcatttt tgtgcgtaac ctcaatatta tcgccttctc tatcctttta acagtactgt
     2881 cacaagacaa accagtcttc tccgttttca cctacttgtt tctctcataa ataattctaa
     2941 gcccttccag ggtcagaagt ccgttggttt cgtcaatggt ctcggattca ctggcaataa
     3001 gtcttgccat tccgcctgta gcaattacct ttatattgct ttccttcatt tcctgtttca
     3061 ttcgctttac aatataatca acctgcccga catatccgta taccatcccc gactgcatac
     3121 tggaaacggt gttgcgcccg ataacagttt tgggctttga aagatctatt ctcgggagtt
     3181 tcgcagtttt ttcgaacaat gcttccgacg atatctgcat tccgggacag ataactcccc
     3241 caatgtattc acaccgcgat gaaatagcac aaaaagtagt tgcagtgccg aaatcgacaa
     3301 ttataacagg ccctccatat atttcaaatg ccgccacagc attgacaatc ctgtctgcac
     3361 caacctctct ggggttttca tacattatat taatgccggt tttaatccct ggcccgacta
     3421 ccatcggtgt cttttgaaaa tactttctaa tggcatgctc tatagagtac attatgggtg
     3481 gaactactga agctatcatt actgcttcta ctttttcaat atcaatcttt tcataattga
     3541 aaaggctaag cagaaacatt cccatctcat ccgaagtttt tccccggtcg gtactcattc
     3601 tccaatgatt tataagcttc tttccgtcat atacacccaa tacagtgttg gtatttccaa
     3661 catccattac cagtatcatt ttctcctccc caatgtctaa actccaagct tcaatcctct
     3721 tatttctttt atttcaagca ttatttcctt ctcaatcctt actatatccg atttaatatc
     3781 ttcaacagtt tcttcagtct ttgacctgcc ggaatcccga tatgaataac cgctgcctgt
     3841 atttttcgac tgtccgctgt ctctcttaaa gctatttccc ttatgaaagt tattgtccct
     3901 ttggtgactt gtgtccttgc cggaatagga gcttctcgat gttgaagcac cgtccttgtt
     3961 ctgcttgccg gtcttattgt tggacctgtt tccttttaat atgtttccgg gtttatttga
     4021 gaaatcccct ctgctgttgt ccttacttgc cgcttcgctt ccgttcttct tgtaaaaacc
     4081 ctttttcctt ttattcctgt tgtttaaccc gccgttatta tcattcccgt caaaattacc
     4141 gttgatgttg ctcatgctgc cattgtttcc gttgttatta tcaaccacag ataatccccc
     4201 ttcgaaatag tatatcctcg tcatcaggta tcaaagcaga aa
//
LOCUS       DAOOJC010000202         4234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16332_length_4234_cov_3.790141, whole genome shotgun sequence.
ACCESSION   DAOOJC010000202 DAOOJC010000000
VERSION     DAOOJC010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16332_length_4234_cov_3.790141"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLA01_06385"
     CDS             <1..580
                     /locus_tag="PLA01_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPD00962.1"
                     /translation="DALGYDSVHLVGASMGGMIAQTVAIEYPHRVRSLTSIMSTTGDP
                     SVGQPDYSVLANLGSPPYDDRQAYIDWQIKSRKALGSPKYPLDEKVTAEVAGLAWDRN
                     HDPLGMMRQAVAVIKSGDRTEKLRSMKIPTLVIHGENDKMIDISGGRAVAAAIPGAEL
                     ATFEGMGHELPKPLWSAFAAKIANLIHRAEPS"
     gene            701..1567
                     /locus_tag="PLA01_06390"
     CDS             701..1567
                     /locus_tag="PLA01_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPD00963.1"
                     /translation="MKKLFILTVAILMLCSFILTGCNSGGGSSNGKSSKAKVIDIPLT
                     EEEYAFGVDKSQPELLSKVNEFISKIKSDGTLEEISNKYFGEGEPAAVASATEDSSKD
                     QLIVATNAAFEPFEYTKGDKYYGIDMEIAALLAEHLGKELVIKNMDFDAVCLSVGQGK
                     ADIAMSGLTIKEDRKEYVTFSDKYYDASQKLIVKASDNTFDNCKTAADVEAILSELKS
                     DTKIGVQTGTTAQFYVEGDEDWGFEGYNVKCVGYKSGSLAVQDMLNGNINYVIIDEAP
                     AQSIVKAINELN"
     gene            1636..2346
                     /locus_tag="PLA01_06395"
     CDS             1636..2346
                     /locus_tag="PLA01_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPD00964.1"
                     /translation="MGNFDKKLMKFWEIFYENNGYTKVLVGLKNTLFIAIFGLLLGII
                     IGTIIAIIKVVPKYKKLPQILNALCDFYVGLFRGTPVVVQLLVAYYVILPLLGMNISA
                     LTVCVLVFGLNSGAYVSEIMRGGIMSVDPGQMEAGRALGLSYRVTMLKVVVPQAVKNI
                     LPTLGNEFIALIKETSVVSFVGAADLYVAFNYIGTNSYEFMVPYLVMALIYIVLVMII
                     ASLIKVMERRLRKSDRRN"
     gene            2330..3154
                     /locus_tag="PLA01_06400"
     CDS             2330..3154
                     /locus_tag="PLA01_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HPD00965.1"
                     /translation="MIDVINLKKRYGSLEVLKGITISIKEGEKVAIIGPSGSGKSTFL
                     RCLNCMEDPTSGSVIFEGVDIADMKVDINVYRRDMGMVFQQFNLFNNKTVIENIMLAP
                     VYIGIHDLKVKKYKNFFIKLRNTFAKQKKPLYEINTTREKIKIDVRENAMRLLKRIGL
                     EDKADVYPSTLSGGQKQRIAIIRALAMNPKVMLFDEPTSALDPEMVGEVLDLIKQLAE
                     EGMTLVIVTHEMGFAREIATRVLFMDEGRILEENTPEQIFSNPQNPRTKEFLSKVL"
     gene            3373..3690
                     /locus_tag="PLA01_06405"
     CDS             3373..3690
                     /locus_tag="PLA01_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPD00966.1"
                     /translation="MDNNLPLTEAIFYILLAVRKPNHGYGIIQEVSEVTGGRLVLGPG
                     TLYGAINSLLSKRWIMLYSEDKTSRKKKEYLITKDGKEIFFEEVKRLEELVQNAKLME
                     ESV"
     gene            3687..4187
                     /locus_tag="PLA01_06410"
     CDS             3687..4187
                     /locus_tag="PLA01_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HPD00967.1"
                     /translation="MKKFRLYYDKDVEQDWLQKMSLNGWALENFFLGVYTFVPCKPGE
                     YIYQIDILDNWGGDKEDFAAFMEDSGVEVVSQWYRWVYLRKKADDGPFEMYTDIDSKI
                     DQYSRIKNFFQVVLIIEVICLFMEIVAAIKTGNPLFWLLTIIIGFITLAILRVVWGSQ
                     WKIDPM"
BASE COUNT         1360 a          683 c          927 g         1264 t
ORIGIN      
        1 ggatgcactt gggtatgaca gtgtacactt agttggtgca tcaatgggcg gaatgatagc
       61 tcaaacagta gctatagagt atccccacag agtgaggtca cttacttcca taatgtcaac
      121 tactggtgat ccttctgtag gacagcctga ctattcggtg ttggcaaacc tgggttcacc
      181 accatatgat gaccgacaag cctatataga ttggcagatt aaatcacgca aagctctcgg
      241 ttctcctaaa tatcctttag atgaaaaagt tactgccgaa gtcgcaggtc ttgcctggga
      301 caggaatcat gatccgctgg gtatgatgcg gcaagctgtt gcagttataa aatccggtga
      361 cagaacggaa aaacttcgtt ctatgaagat tccgactctt gttatccacg gtgaaaatga
      421 taaaatgatt gatataagcg gaggacgtgc tgttgctgcc gctattccgg gtgctgagct
      481 tgccacattt gaaggaatgg ggcatgaact gccaaaacct ttatggtcag cgtttgcagc
      541 aaaaattgca aatctgattc atagggcaga gccctcatga tatgttatac ctttaaccca
      601 aacactctat gttgacaaaa acgccgggat acgataattt atatttaaat aacaaatgct
      661 aaaaatataa gtttataaag attttaagga gaatgttgtt atgaaaaaac tatttatttt
      721 aactgttgca attttgatgc tttgcagttt tattcttaca ggctgtaatt cgggcggcgg
      781 aagttcaaac ggtaaaagtt cgaaagctaa agtcattgat attccgctga ctgaagaaga
      841 atacgctttt ggagtggata aaagccaacc ggaactgctt tcgaaggtaa atgaatttat
      901 atcgaaaatt aaatccgatg gaaccttaga ggaaataagc aacaagtatt tcggagaggg
      961 agaacccgca gctgttgcat cggccacaga agacagcagc aaagatcagc ttatcgtagc
     1021 tacaaatgcg gcatttgagc cctttgaata tactaaaggc gataagtact acggtattga
     1081 catggaaatt gctgcacttc ttgcagagca ccttggaaaa gagctggtaa tcaaaaacat
     1141 ggattttgat gctgtttgcc tttctgtagg tcagggaaaa gccgacattg cgatgtctgg
     1201 tcttacaata aaagaggaca gaaaagagta tgttaccttc tccgacaaat attacgatgc
     1261 ttcccaaaag cttatcgtta aggcaagcga caataccttt gacaattgta aaactgcagc
     1321 tgatgtagaa gccatcctgt cggaactgaa aagcgatacg aaaattggtg tgcagacggg
     1381 tacaaccgcc caattctatg ttgaaggcga tgaagattgg ggctttgaag gttataatgt
     1441 taaatgcgta ggttacaaat caggttctct ggctgttcag gatatgctta acggaaacat
     1501 caactatgtc attattgatg aggcccctgc tcaaagtata gtgaaagcaa ttaacgaact
     1561 taactgaatt aaataaagtt catacaatac tccgcaaaat attttgcggg tattgtatta
     1621 tataggggat taattatggg aaattttgat aaaaaattaa tgaagttctg ggaaattttt
     1681 tatgaaaaca acggttacac aaaagtcctt gtgggactta aaaataccct ttttattgca
     1741 attttcggac ttttattggg aattattatc ggtacgataa ttgctattat taaagtggtg
     1801 cccaaatata aaaaattgcc ccaaatatta aatgccctat gtgattttta tgttggatta
     1861 ttcagaggta caccggttgt tgtgcagctt ttggtcgcct attatgtaat tttgcccttg
     1921 cttggcatga atatatctgc acttactgtc tgtgtattgg tattcggtct caatagcggt
     1981 gcttacgttt ccgaaataat gagaggcgga attatgtctg tagatcccgg acagatggag
     2041 gcaggccggg ctcttggcct cagttatcgc gtcacaatgc tgaaagtagt tgttcctcag
     2101 gctgtcaaaa atatacttcc tacactggga aatgaattca tagccttgat taaggaaact
     2161 tccgttgtta gttttgtcgg tgctgccgac ttgtatgtcg catttaatta tattggcaca
     2221 aacagttatg aatttatggt tccatacctt gttatggcac ttatttatat tgtattggtt
     2281 atgataatag catcgctcat aaaagtgatg gaaaggaggt tgaggaagag tgatagacgt
     2341 aattaatcta aagaaaagat atggcagcct tgaagtttta aaaggcatta caattagcat
     2401 caaagagggt gaaaaggttg ccatcatagg tccgtcggga agcggtaaaa gcacttttct
     2461 tcgttgcctc aattgtatgg aggaccctac gagtggatcg gttatatttg aaggtgtaga
     2521 tattgcagat atgaaggttg atattaacgt ttaccggcgt gatatgggta tggtttttca
     2581 gcaattcaac ctgttcaaca ataagactgt cattgaaaat attatgcttg ccccggtata
     2641 tataggaata catgatctta aggtaaaaaa gtataagaat ttctttataa aacttagaaa
     2701 tacttttgcc aaacaaaaaa agcctctgta tgaaatcaat actactcgtg aaaagataaa
     2761 gatagatgta agggaaaatg ctatgcggtt gcttaaaaga atcggacttg aagacaaagc
     2821 tgatgtttac ccgtcgactc tttccggggg acagaagcaa agaatagcaa taattcgtgc
     2881 ccttgcaatg aaccccaaag tcatgctgtt tgatgagcct acttcggcgt tagaccccga
     2941 gatggtcggg gaggttcttg accttatcaa gcagcttgca gaagagggta tgactttggt
     3001 tatcgtaact catgaaatgg gttttgcaag agaaatagct accagggttt tgtttatgga
     3061 tgaaggaaga attctggagg aaaacactcc cgaacagatc ttttcaaacc ctcaaaaccc
     3121 cagaacaaag gaatttttat cgaaggtatt ataatactta cagggtattc tatttattta
     3181 ccggttaaaa ccttacatca tcatttcctg tttttaaaag aactaatctt acctttctcc
     3241 atgtgactga aatgaataag tcatatatta agacttattt atttcagaaa ttttactttt
     3301 tcatattgac aaatatatcg tcagatgata tatttaatat atcgagacac gatagatagc
     3361 gaggtgatat atttggacaa taatttacca ttgactgaag caatatttta tattctgctt
     3421 gcggtaagaa aacccaatca tggatatgga attattcaag aagtaagtga ggttaccggc
     3481 ggtagattgg tactcggtcc cggcacctta tacggtgcta taaattcttt gctgtccaaa
     3541 agatggatta tgttatacag cgaagataag acttctagaa aaaagaagga atatttgata
     3601 acaaaggacg gtaaagaaat attttttgaa gaagtaaaac gactggaaga gcttgttcaa
     3661 aatgcaaaat taatggagga gtctgtatga agaagtttag attatattat gataaggatg
     3721 tagaacagga ttggctgcaa aaaatgtcat taaacggatg ggcgttagaa aactttttct
     3781 taggtgtata tacattcgtt ccctgtaaac cgggtgaata catctatcaa attgatatac
     3841 tggataattg gggcggagat aaagaagatt ttgctgcatt tatggaggat agcggtgttg
     3901 aagtggtttc acaatggtat agatgggttt acctcagaaa aaaagctgac gatggtccct
     3961 ttgagatgta tacggatata gactctaaaa tagaccagta cagtagaata aagaattttt
     4021 ttcaagttgt attgataata gaagttatat gtttatttat ggagattgtt gcagccataa
     4081 aaacaggaaa ccctttattt tggcttttga caataatcat aggctttatt actctggcaa
     4141 ttcttcgagt agtatgggga agccagtgga aaatagatcc tatgtaatgc tttgtactat
     4201 aatagcctag tgcataaaaa accagagtaa ttcc
//
LOCUS       DAOOJC010000203         4232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16344_length_4232_cov_3.593967, whole genome shotgun sequence.
ACCESSION   DAOOJC010000203 DAOOJC010000000
VERSION     DAOOJC010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16344_length_4232_cov_3.593967"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1858
                     /locus_tag="PLA01_06415"
     CDS             134..1858
                     /locus_tag="PLA01_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD00968.1"
                     /translation="MKKLLVYLKGYIKESILGPLFKLLEASFELLVPIVIKCIVDIGI
                     GQANSGYVVKMCLLLVLLGIVGMICSITAQYFAAKAAVGFTTKLRRALFEHIGQLSYS
                     EIDTLGTSSMITHMTSDMNQVQSGLNLALRLLMRSPFIVFGAMIMAFTVDAHAALTFV
                     VAIPALSAVVFAIMLLSIPLYKKVQQQLNKVLNSTRENLTGVRVIRAFRLEDKEIAEF
                     DKRNEELTSIQKFVGGISALMNPLAYVTINLAIIWLIHTGAIRVSTGLLTQGAVLALY
                     NYMSQILVELIKFANLIISITKGFASANRISAVFDMKSSLKEKDVPLRNLHSEYAVEF
                     RNVGLRYKNSRDESLTNISFCVRRGEVVGIIGGTGSGKSSLINLIPRFYDATKGEVIV
                     DGINVKDYPIRKLRDKIGIVQQKAVLFKGSIRDNMHWGNKDATDEEIMEAITIAQAGE
                     IVAQKKEGLDFIIEQGGKNLSGGQRQRFTIARALVKKPEILILDDSASALDFATDAAL
                     RKALREMPGNTTVFIVSQRTSSIQHADIILVLDDGQITGIGKHEELLEKCEVYREIYD
                     SQFKKEAK"
     gene            1860..3620
                     /locus_tag="PLA01_06420"
     CDS             1860..3620
                     /locus_tag="PLA01_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD00969.1"
                     /translation="MKKQKVQINNLRKVLKYIKEYRILMLISIALAAVTVALTLYVPI
                     LIGRAIDCIVGPGAVDFDKVTEILFKIGFTIAITAVSQWLMNQINNKITYHVVQDVRK
                     KAFRKIEVLPLSYIDAHSHGEIVSRIISDVDQFADGLLMGFSQLFTGVVTILATFIFM
                     LTINVQITFIVVILTPLSLFVADFIAKRTYSMFKLQSETRGEQTSFIDEMIGNSKIVQ
                     AFSYQEEALKRFDEINERLKKHSLRAVFFSSLTNPCTRFINNLVYAAVALAGGLAVIG
                     GNTEGVMTVGGLSIFLSYANQYTKPFNEISGVITELQNALACAGRVFELIEEQPQIPD
                     SNNAVILEKANGNISMENVAFSYVTERPLIRDFNLKVKPGQRVAIVGPTGCGKTTVIN
                     LLMRFYDVNSGSIKVEGIDIRNITRKSLRENYGMVLQDTWLKSGTIRENITIGKPEAT
                     DEEIIAAAKAAHAHSFIKRLPNGYDTVIGEDGGSLSQGQKQLISIARVMLCLPPILIL
                     DEATSSIDTRTEIKIQEAFAKLMQGRTSFIVAHRLSTIREADIILVMKDGDIIEQGTH
                     EELFSQKGFYANLYNSQFAL"
     gene            complement(3761..>4232)
                     /locus_tag="PLA01_06425"
     CDS             complement(3761..>4232)
                     /locus_tag="PLA01_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00970.1"
                     /translation="EDLQNIELLPLDIAQDNEIIWGTHPIVDTMILEEKDIDLPFLIE
                     PLKGDKKNKVKITWGTAIVELDAQEVPELLKYKTDTYGVSLTMNFNYLLSKHGYRSDS
                     YTDASKYIHIAELKRKLAEYLGEDENFDMDDFAERFGGLTRKQYIKLAYERFKK"
BASE COUNT         1226 a          878 c          866 g         1262 t
ORIGIN      
        1 agatttttag ggtgtattaa ataaatgtga agaaaatgta caccagattt ttttcttaaa
       61 cgtgcagcac agtaaaaatc atgctacaat atattagttg tatgcaatag ataccctttt
      121 tggaggacgt tccgtgaaaa agctattggt ctatttaaaa ggatatataa aagaaagtat
      181 attaggtccg ctgttcaaac tgctggaagc atcctttgag cttttagtgc ccattgttat
      241 caaatgtatc gttgacattg gtattggaca agccaacagt ggatatgtcg ttaagatgtg
      301 tctgttgctg gtacttttgg gtattgtggg aatgatatgt tcgataactg cccaatactt
      361 tgcggcaaaa gcagcagtgg gttttacaac aaagcttcgc cgggcactgt ttgaacatat
      421 cgggcaatta tcctattccg aaattgacac cctcggtaca tccagtatga ttacccatat
      481 gaccagcgat atgaatcagg tgcaatccgg cttgaacctg gccttgcgtc tgttgatgcg
      541 ttcccccttc attgtgtttg gggccatgat tatggccttt accgtagatg cccatgcagc
      601 acttaccttt gtagtggcaa ttccggcact ttctgcagtt gtttttgcca taatgctatt
      661 atccattccc ctgtataaaa aggtacagca gcagttgaac aaggtattga actcgacgag
      721 ggaaaacctt acaggtgttc gtgtaatcag agcttttcgc ctggaagata aagaaatagc
      781 cgagtttgac aaacgcaatg aagaactgac ctcaatacag aaatttgtag gcggaatttc
      841 tgcactgatg aatcctttgg cctatgttac tattaacctt gcaattattt ggcttataca
      901 taccggtgct atacgggtat ctacaggcct gcttacacaa ggtgctgttc ttgcacttta
      961 caattacatg tcccagattc ttgttgaact aatcaagttt gcaaacttaa tcatcagcat
     1021 tacaaagggt tttgccagtg ctaaccgaat cagtgctgtt ttcgatatga aatcaagcct
     1081 taaggaaaaa gatgtgccgc ttagaaatct tcactctgaa tatgctgtag aattccgtaa
     1141 tgtcgggtta cgatacaaaa attcccggga tgaatcattg acaaatataa gcttctgcgt
     1201 ccgccgcggt gaggtagtcg gaataatcgg cggcacaggt tcaggtaaat cctctcttat
     1261 taatttgatt ccaaggtttt atgatgccac caaaggagaa gtaattgtag acggtattaa
     1321 tgttaaggat taccccataa gaaagctgcg ggacaaaatt ggtattgtgc aacaaaaggc
     1381 tgtattgttc aaaggcagca tacgggataa tatgcactgg ggtaataagg atgcaaccga
     1441 tgaagagatt atggaagcca ttacaatagc ccaagccggt gaaattgttg ctcaaaaaaa
     1501 agaagggctg gactttatta tcgaacaagg cggcaaaaat ctttccggtg gccagcgtca
     1561 acgctttacc atagcccgtg cccttgtaaa gaagcctgag attttaatcc ttgacgacag
     1621 tgcttcagcc ttagactttg caacagacgc agcacttcgc aaggctctgc gtgaaatgcc
     1681 gggaaacacg accgttttca ttgtttcaca acgaacctca tccattcagc atgccgatat
     1741 aatccttgtc cttgatgatg ggcaaataac tggtatcggt aagcatgagg aactgcttga
     1801 aaaatgcgag gtatatcgtg aaatttatga ctcacagttc aagaaggagg caaaatgata
     1861 tgaaaaagca gaaagttcaa attaataacc tccgaaaggt cctcaaatat atcaaggaat
     1921 accgtattct catgttgatt tccattgctc tggctgctgt tacggtagca ctgacccttt
     1981 atgtgcccat tctcataggc agagccattg attgcatcgt tggacctggg gctgtagact
     2041 ttgacaaagt gaccgaaatt ctttttaaaa tcggttttac tattgctatt actgctgttt
     2101 cccaatggct gatgaatcaa ataaacaaca aaattactta ccatgtggtg caggatgttc
     2161 gtaaaaaagc ttttcggaaa attgaagttc tgcccctttc gtatattgac gcccactccc
     2221 acggcgaaat cgtgagccgt attatcagcg atgttgacca gtttgccgat ggacttctga
     2281 tgggattttc acagctattc acaggtgttg tcacaatttt ggccacattt atcttcatgc
     2341 tcaccatcaa tgtacagatt acttttattg tagttattct tacacctctt tctctatttg
     2401 ttgcagactt cattgcaaag cgtacctatt ccatgttcaa gctgcagtcg gaaacccgtg
     2461 gagagcagac ctcgttcatt gacgaaatga taggcaattc aaaaattgtc caggcctttt
     2521 cctatcagga agaagcactc aaaaggtttg acgagattaa tgaacgattg aaaaagcact
     2581 cccttcgggc tgttttcttt tcatccctta ccaatccctg cacccgtttc ataaacaatc
     2641 tcgtatatgc agcagttgcc cttgcgggcg ggcttgcggt catcgggggt aatacagaag
     2701 gtgtaatgac ggtgggtggg ctttccatct tcctcagcta tgccaaccaa tacacaaaac
     2761 cttttaatga aatctccgga gtaatcaccg agcttcaaaa tgcccttgct tgtgcggggc
     2821 gtgtgtttga gttgattgaa gaacagcctc aaattcccga tagcaacaat gcagttattt
     2881 tagaaaaggc caatggaaat atctcaatgg aaaatgtagc tttttcctat gttaccgagc
     2941 gtcccttgat tcgtgatttc aatctgaaag taaaacccgg tcagcgtgta gctattgtgg
     3001 ggccgaccgg ctgcggtaaa actactgtta ttaatctcct gatgcgtttt tacgatgtca
     3061 attccggaag tataaaagtt gaaggcatag atattcgtaa cattacccga aagagtttaa
     3121 gagaaaacta cggcatggta ttgcaggaca catggctgaa atccggtact attcgggaaa
     3181 atattacaat aggcaaaccc gaagcgacgg atgaagagat cattgctgcg gcaaaggctg
     3241 cccatgccca cagctttatc aaacgcctac ccaatggtta tgatactgtc atcggtgaag
     3301 acggcggaag tctttcccag ggacaaaaac agctaatcag cattgcccgg gttatgcttt
     3361 gtttgccacc gatactcatt ttggacgaag caacatcttc catcgatacc cgtactgaaa
     3421 tcaaaatcca ggaagctttt gcaaaactta tgcaaggacg gaccagtttt attgttgccc
     3481 accgtctgtc caccattcgg gaagcagata tcattctcgt tatgaaagat ggggatatta
     3541 ttgagcaggg cactcatgag gaactgttct cgcagaaagg cttttatgct aatttgtata
     3601 acagtcaatt tgccctttaa attaaaagta gccttacttt tatggtaatt cacgagtaag
     3661 gctacgtttc acaatatctt ataaaaatac tccaaaagca aacgccaacc ttaagtatat
     3721 tggcatattc tcataagctt taaagcttgt tcttctgtcc ttatttctta aatctttcat
     3781 acgcaagttt tatatactgc tttctggtta gtcctccaaa cctttcggca aaatcgtcca
     3841 tatcaaaatt ttcatcctca ccgagatatt ctgccaattt tctttttaac tccgcaatat
     3901 gtatgtattt agaggcatcg gtatatgaat ccgatctata accgtgtttt gaaagcaaat
     3961 agttaaaatt catagttaaa ctcacaccat aggtatctgt tttgtatttt aataattcag
     4021 gcacctcttg agcatccaat tctactattg cagttcccca agtaattttc actttatttt
     4081 tcttatcacc ctttaatggt tcaatcaaaa aaggaagatc aatgtccttt tcttctaaga
     4141 tcatggtatc tacaatcggg tgagttcccc aaataatttc attatcctgt gcaatatcca
     4201 aaggaagcag ttcaatattc tgcaagtctt cc
//
LOCUS       DAOOJC010000204         4224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16382_length_4224_cov_4.349964, whole genome shotgun sequence.
ACCESSION   DAOOJC010000204 DAOOJC010000000
VERSION     DAOOJC010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16382_length_4224_cov_4.349964"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1263)
                     /locus_tag="PLA01_06430"
     CDS             complement(124..1263)
                     /locus_tag="PLA01_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD00971.1"
                     /translation="MIDFKKELQNYPPIDIDKLSATNPDMPDSMKNSIILYNKALEKF
                     RVGSEDIAIIELRKAISLNPDFYEAYNLLGVFYIYTKEYDNAAQVFKKVIDAEKNSII
                     ALNYLKKIDSSYEPQVKKQTKEKKEKGNAAKDRGKTVKVEEKAPLSLNKPARKENYKS
                     KIVLGTFIGFIFGVLLVFVPSFKFYSREDNTEKINQLTAEKEAAVLKIGEYEKKYDEL
                     DNKYNGLSTQFEEVSKQADYYLSVAKILNIEKLISENKISEAADEILLLKNKNFQGVE
                     KDRFDKLVKDILPRAAQSEYNAGRELYNKKKYQEALDRFIKSKVYYEDWKYSVNNLFY
                     CGICYQELNNNTMAVKVFEELIEKYPSSTYAQQSQNRITQIQNNQ"
     gene            complement(1374..2654)
                     /locus_tag="PLA01_06435"
     CDS             complement(1374..2654)
                     /locus_tag="PLA01_06435"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPD00972.1"
                     /translation="MDYDRAIEYIHGTLKFGVKLGLENIKALLNLMDNPHKKLKYVHV
                     AGTNGKGSTVAFISSILIESGYRVGIYTSPYIQRFTERIRVNNDEISKQDLARIASFV
                     KEKVEVMVSRGLNHPTEFEIVTAIAFQYFFERNCDIVVLEVGLGGRFDSTNVIDNPLV
                     SVITTISYDHIDILGNTLSEIAFEKAGIIKDGSDVVLYPQARDAERVFEEVCRERNAK
                     LHKVSFDTVKILGFSIEGQEFDYKLLKSLKIGLLGEHQIKNAVTAIETVSVLSEIGYD
                     IGEDSIRKGLISAQWPGRLEVLCKKPVLIIDGAHNLEGVQALVKFLKEYLPQKKIVFI
                     IGILKDKDYISIIEEVVPFAEHIIAVTPQNKRALPARELLEFIKPYCNNISASDTIEE
                     AVKKSFEITPEDGLICAFGSLYYIGEVRSIFSLS"
     gene            complement(2731..>4224)
                     /locus_tag="PLA01_06440"
     CDS             complement(2731..>4224)
                     /locus_tag="PLA01_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HPD00973.1"
                     /translation="VRKDHNDSVYKTERGKFNAVIKQVVECHEKGQPVLIGTISIEKS
                     EMLSLVLKKRGIPHQVLNAKYHEKEAEIIAQAGKFGAVTIATNMAGRGTDIVLGGNAE
                     YMAKQEMRKKGFSEELINQATGFNDTFDAEVLNSRKVFRELYEEFKKITNTEREKVVE
                     AGGLFIIGTERHESRRIDNQLRGRSGRQGDPGESRFYISLEDDLMRLFGSDRLMGMVN
                     ALGLEEDEPIERKMLSNAIETAQKRVEGKNFAIRKNVLEYDDVMNKQREVIYSQRRKV
                     LDGENLKDNIIKMIEGIAEGIVNLYCGESPHPDDWDWEGMRSYAEKSYVTEGTLNFSM
                     DQMERMTKASLKESILENMLKRYEEKEQEFGSELMRELERVVLLRIVDQKWMDHIDDM
                     DQLQHGIRLRAFGQKDPVIEYKFEGFEMFEEMNRNIQADVVKVILNTHIDRNRVPQRE
                     KVAEPVTESYGDDGVKKPVVKKQKVGRNDLCPCGSGKKYKKCCGIDE"
BASE COUNT         1122 a         1038 c          619 g         1445 t
ORIGIN      
        1 tccaccgcct cccaacacaa ggttcgccct tattcggttt ctttccatgt ggtaatgcct
       61 cacatttgaa aataactatg ttatatatat taatcaaatt atacaaatag tatttttttt
      121 gagctactgg ttgttttgaa tctgtgtaat gcggttttgt gattgctgtg cataggtact
      181 ggacggatat ttttcaataa gctcttcaaa tacctttacc gccatggtgt tgttattaag
      241 ctcttgataa catattccgc aataaaacag gttattgacc gaatacttcc aatcttcgta
      301 ataaaccttt gacttaataa acctgtccaa agcttcctgg tatttctttt tattgtatag
      361 ctctctgccc gcattatact cactctgagc agctctcggc aaaatatctt tcacaagctt
      421 atcaaatctg tctttttcca ccccttgaaa gtttttattc ttcaagagca aaatctcatc
      481 agccgcttca ctgattttat tctcggaaat aagcttttca atatttaata tcttcgcaac
      541 gctcaaataa tagtcagcct gcttgcttac ttcctcgaac tgggtgctta gcccgttata
      601 cttattatcc aactcgtcgt attttttctc atattcgcct atctttaaaa cagcggcttc
      661 cttctccgca gtcaactggt ttattttttc cgtattatcc tctcttgagt aaaacttaaa
      721 gcttggtaca aaaacaagca aaaccccaaa aataaatcct ataaaagtgc ccaacactat
      781 tttggacttg tagttttctt ttctcgccgg cttattcaag gaaagcggag ctttttcttc
      841 aactttaacc gtctttcccc tgtcttttgc agcattgccc ttttcctttt tttcttttgt
      901 ctgtttcttc acctgaggct catagcttga atcaattttc ttcaaatagt taagggctat
      961 tatgctgttc ttctcagcat ctataacttt cttaaaaacc tgtgcggcat tatcgtattc
     1021 cttagtgtaa atatagaaaa ctcctaaaag attataggct tcataaaaat cgggatttag
     1081 agaaatggct tttctcaact caattatagc aatatcctcg ctgcctacgc ggaacttctc
     1141 taaagcctta ttgtacaata tgattgagtt tttcatactg tcaggcatat ccgggttggt
     1201 cgcggataat ttgtcaatgt caatcggcgg ataattttgg agttccttct taaaatcaat
     1261 cattaattcg cctcctctca caatttatct ggcacagtgc ttctcatata tttactcaat
     1321 gaaaatcctg cgaattacca cattgattcg acatattgtg cagtgaactt tatttatgat
     1381 aatgaaaata tgctccttac ctcacctata taatataatg aaccgaaagc acaaatcaag
     1441 ccatcttccg gagttatttc aaaacttttt tttactgcct cttcgattgt atcacttgcc
     1501 gatatattat tacaataagg tttaataaat tccaacagtt ctctggccgg caaagctctt
     1561 ttattttgcg gtgtaacggc tattatatgc tccgcaaaag gcacaacttc ttcaattatc
     1621 gatatatagt ccttgtcctt taatataccg ataataaata ctattttttt ctgcggtaaa
     1681 tattccttca agaacttaac caatgcctgc acaccttcca aattgtgagc cccgtcaatt
     1741 attaaaaccg gttttttgca caacacctca agccttccgg gccattgggc actgattaat
     1801 ccttttctta ttgagtcctc accgatatca tatcctattt ccgaaagaac agataccgtt
     1861 tctatagcag tcactgcgtt ttttatctga tgttctccca acagacctat ttttaaagac
     1921 ttcaacaact tatagtcaaa ttcctgccct tcaatggaaa atcctaatat tttaactgta
     1981 tcaaaggaaa ccttgtggag tttagcattt ctttccctgc aaacctcttc aaaaaccctt
     2041 tcggcatccc tggcctgggg atataacacc acatcggacc catcctttat tattcctgct
     2101 ttctcaaaag caatttcaga gagggtattc ccaagtatat cgatgtggtc atagcttata
     2161 gttgttatta ccgagaccag cggattatct ataacattgg tggaatcaaa cctgcctcca
     2221 agtcccacct ccagaaccac aatatcacaa ttcctttcaa agaaatactg aaaagcaatt
     2281 gcagttacta tctcgaactc cgtcggatgg ttaagccccc tcgagaccat aacctccacc
     2341 ttctctttca caaacgatgc tatcctggcc aggtcctgct tcgaaatctc atcgttatta
     2401 acccggatcc tttctgtaaa cctttgtatg tagggcgagg tatatattcc gaccctatag
     2461 cccgattcaa tcagtatgct gcttatgaaa gcaactgtcg aaccttttcc gttcgtaccc
     2521 gcgacatgta catatttaag ttttttatgc ggattgtcca tcaaatttaa aagggctttt
     2581 atgttttcaa gtcccaattt tactccaaac tttaatgtac cgtgtatata ttcaatcgcc
     2641 cggtcataat ccataacaac atacctcccc aaaattgcaa aaaggggtta taaaaacccc
     2701 taaaaaagat ccaatatatt caataaactg ctattcatct attccgcaac actttttata
     2761 cttctttccg cttccgcagg ggcaaagatc gtttcgtccc accttctgct ttttaacaac
     2821 gggtttcttg acaccgtcat ccccatagga ttctgtcaca ggttccgcca ctttctcacg
     2881 ctgcggaaca cggttgcggt caatatgggt atttaaaatt actttcacta cgtccgcctg
     2941 gatattccgg ttcatttcct caaacatctc aaagccttca aatttgtatt caataacagg
     3001 atctttctgt ccaaaagccc tgagccgtat accatgctgc agttgatcca tatcgtcaat
     3061 atgatccatc catttttggt cgacaatcct taaaagtaca actctttcca actctctcat
     3121 aagttcggaa ccaaactcct gttccttctc ctcatacctt ttaagcatat tctcaaggat
     3181 tgattctttt aatgacgctt ttgtcattct ctccatttga tccattgaga aattaagggt
     3241 tccttcagtc acataagact tttcagcata agaccgcatg ccttcccaat cccagtcgtc
     3301 aggatgtggg ctttcaccgc agtataaatt aacaatacct tctgcaattc cttcgatcat
     3361 ttttattata ttatctttga ggttctcacc atccaaaacc tttcttcttt ggctatatat
     3421 tacttctctc tgtttattca taacatcatc gtattcgaga acgtttttcc ttatggcaaa
     3481 gttcttgcct tcaaccctct tttgtgcagt ctcgatagca ttactcaaca ttttacgttc
     3541 aataggctca tcttcttcca aacccaatgc atttaccatg cccatcagcc tgtcggatcc
     3601 aaaaagcctc ataaggtcat cctcaaggga tatatagaat ctggactccc ctggatctcc
     3661 ctgtctgccg gaacggcctc ttaactggtt gtcaatacgt ctggattcat gtctttcagt
     3721 acctataata aacagtccac cagcctcaac aaccttctcc ctttcggtat ttgtaatttt
     3781 tttaaactct tcataaagtt cccggaacac ttttctcgaa ttcagtacct ctgcatcaaa
     3841 ggtatcgtta aaccctgtag cttgattaat gagttcttcg ctaaagccct tctttctcat
     3901 ctcctgtttt gccatatact cagcattacc acccaaaaca atgtccgttc cccggccggc
     3961 catgttggtc gcaatggtaa ccgcaccgaa ttttcctgcc tgggcaataa tctctgcctc
     4021 tttttcatgg tatttcgcat tcagaacctg gtggggaatc cctcgttttt ttagtactaa
     4081 acttagcatc tcagactttt caatggatat cgtacctata agaaccggct gccctttttc
     4141 gtggcactca acaacttgtt ttataacagc attaaacttt cccctctcgg ttttatatac
     4201 actgtcgtta tggtccttcc ttac
//
LOCUS       DAOOJC010000205         4190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16541_length_4190_cov_3.623216, whole genome shotgun sequence.
ACCESSION   DAOOJC010000205 DAOOJC010000000
VERSION     DAOOJC010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16541_length_4190_cov_3.623216"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLA01_06445"
     CDS             complement(<1..182)
                     /locus_tag="PLA01_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPD00974.1"
                     /translation="MSHYYTQNPDTPHDIGSIGYEVNGIKLQLTTDSGVFSKNKVDYG
                     SNLLIQSLPPAAGNIL"
     gene            530..1381
                     /locus_tag="PLA01_06450"
     CDS             530..1381
                     /locus_tag="PLA01_06450"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HPD00975.1"
                     /translation="MSVTVKNLSYVYMKGTAFEKKALDDVSLNIETGEFVGIIGHTGS
                     GKSTLVQHFNGLLKPTAGSVFINGEELKGNRAKELKRKVGIVFQYPEHQLFEETVYKD
                     IAFGLVKRGESKDVIDEKVKKTAKALGLSEELLEKSPFELSGGQKRRAAMAGILVLQP
                     EILVLDEPAAGLDPKGRDEIFGLISGLHKNLKMTVVLVSHSMEDIAKYVDSVIVMNRG
                     RIEMSGPTRSIFSKTDRLEEMGLAVPQITYLLKRLKERIPDINDSIISVSEARKELEK
                     YLRKADG"
     gene            1386..2195
                     /locus_tag="PLA01_06455"
     CDS             1386..2195
                     /locus_tag="PLA01_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPD00976.1"
                     /translation="MFRDITLGQYYPGNSPMHRADPRVKIILTFILMTTIMIAKSYTA
                     FLMIAGLILATVIFCRIPFGYTVKGLKPIMPVVVFAALVNIFTTEGTLLYKFRFIHIT
                     YEGLDTAAKMSLRIVTLIAGTSIMLLTTTPLSLTDGIERLLYPLSRIGLPVHEFAMMM
                     NIALRFIPTLLEETDKVMKAQTSRGVDFETGGIIERGKSFIPVLVPLFVSALRRADEL
                     AVAMEARCYRGGKGRTRMRQLKLSKADLQITFFLAAFEFVLLYVQYFKMPV"
     gene            2347..3297
                     /locus_tag="PLA01_06460"
     CDS             2347..3297
                     /locus_tag="PLA01_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPD00977.1"
                     /translation="MYSLGIDLGGTNIAVGLVNEEGKIIHKDSVPTLRERPYPLIIKD
                     MAMLALKVTEDAGVSIDRISGIGVGSPGTPNCKEGILVYNNNLNFRNVPIRSEIQKYI
                     NLPVYLDNDANCAALAESVAGAAKGAQISVTITLGTGIGGGVVIDGKIYSGFNFAGGE
                     LGHTVIMVDGEQCSCGRKGCWEAYASATALIRQTKKAAQENPNSLINKLIDGDLSKID
                     AKTAFDAAKQGDRTGELVVEQYIKYISEGLINMINIFMPEVLVIGGGVCKEGEYLLKP
                     LRELIKQGVYSKEDIPQTQLRTAQMGNDAGIVGAAMLARE"
     gene            3315..4049
                     /gene="truA"
                     /locus_tag="PLA01_06465"
     CDS             3315..4049
                     /gene="truA"
                     /locus_tag="PLA01_06465"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPD00978.1"
                     /translation="MRNIKLTIEYDGTNYHGWQTQNNARTVQDVIEKAIEGLTGEKVA
                     LIGSSRTDYGVHALGQVANFATNSTIPGDRFCYALNRILPEDIVIRESCEVGLDFHAR
                     FQAKGKKYRYIIYNSRFPSAILRNRAYHVSYNLDIDEMKKAALSFLGTHDFSTFKASG
                     SSVKTSVRTITSVSLEKNGDVLEFEICGNGFLYNMVRIIVGTLVDVGMGRIKADDISD
                     IINGKDRKKAGRTAPAHGLYLVEVYY"
BASE COUNT         1352 a          675 c         1039 g         1124 t
ORIGIN      
        1 tcaagtatgt ttccggcagc gggaggaagg gactggatca ataaattgct gccgtaatcc
       61 actttgttct ttgagaacac accggagtct gtagtcagtt gcagctttat gccattgact
      121 tcataaccga tgctaccaat atcgtgaggt gtatccgggt tctgggtgta ataatggctc
      181 atttttaagt cacctttcaa atataaattt catagtatcg taccacgtac ttattatacc
      241 catcaaggcc tattcaatca atacttaaaa gatgagataa atgtgaagat aaatatggaa
      301 aaagcattaa gtctctggaa caattcactt cacaaatata acaattatgc agaaacaata
      361 aaatcttgag gtgtatcgag tccacatcga ttttggtcat ttcgctctgt gaaattctag
      421 ctacaatatt ttagcattag cttgaagtca aactaaaaaa agtgtataaa taaatatgac
      481 acaaattgag caatctattg tgcaaaagac cgggattgat ggagggaaaa tgtctgtaac
      541 ggtaaagaat ctttcctatg tttatatgaa ggggacggct tttgagaaaa aggccttgga
      601 cgatgtaagc ttaaatatag agactgggga gtttgtcgga ataattgggc acaccggatc
      661 gggaaaatca accctggtcc agcattttaa cggtctttta aagcccaccg cgggaagtgt
      721 ctttattaac ggtgaagagt tgaagggaaa cagggcaaag gaactgaagc gaaaggttgg
      781 aattgtgttt caatatccgg agcatcaatt gtttgaagaa acggtttaca aagacatagc
      841 ttttggtctt gttaaaaggg gcgaaagcaa ggatgtaata gatgaaaagg ttaaaaagac
      901 agcaaaagcc ttgggccttt ctgaagaatt actggagaaa tcaccctttg aattgtcggg
      961 aggacaaaag agaagggcgg caatggcagg gatactggtg cttcaaccgg aaatacttgt
     1021 tttggatgaa cctgcagccg gactggaccc aaaaggacgg gatgaaatat tcggtttgat
     1081 atccgggctg cataaaaacc ttaaaatgac agttgttctg gtatcccata gcatggagga
     1141 tatagcaaag tatgttgaca gtgtaatagt aatgaataga ggaagaatag aaatgagcgg
     1201 tccgacccgg agcattttca gcaagacgga cagacttgaa gagatgggac ttgctgttcc
     1261 ccagataacc taccttctga aaaggcttaa ggaaaggatt ccggacataa atgacagtat
     1321 catatcggtt agtgaggcaa gaaaagaatt ggaaaagtac ttaagaaagg ctgatgggta
     1381 agtaaatgtt tagggatata acactgggac agtattatcc cgggaattca ccaatgcaca
     1441 gggcagaccc gagggtaaaa attattctga catttatatt gatgacaaca ataatgattg
     1501 caaaatcata tacagccttt ttgatgattg caggcttaat cttagcaacc gtaattttct
     1561 gcagaattcc ttttggttat accgtaaaag gtcttaaacc gataatgccg gtggttgttt
     1621 ttgcagcatt ggttaatatt tttacaacag aaggaacttt actgtataag ttcaggttta
     1681 ttcatattac ttatgaaggc ctggatacgg cagcaaaaat gagcttaagg attgttaccc
     1741 ttattgcagg cacatctatt atgctgttga ccaccacacc attgtccctc acggatggaa
     1801 tagaaaggct tctctatccg ttaagccgga taggacttcc ggtacatgaa tttgctatga
     1861 tgatgaatat tgcattaagg tttattccga ctcttttgga ggagacagat aaagtaatga
     1921 aggcacagac atcgcggggg gtggactttg aaaccggcgg aattattgag aggggaaaga
     1981 gttttattcc ggttttggtg cctttgtttg taagtgcgtt aagaagagcc gacgagcttg
     2041 cagtggcgat ggaggccaga tgctacaggg gtggaaaagg aagaacaaga atgagacaat
     2101 tgaagctgtc aaaggcagat ttacaaatta catttttttt agctgcattt gaatttgttt
     2161 tgttgtatgt acaatatttt aaaatgcctg tataatatat gttgaacatg ttgcagcaaa
     2221 gcggcagcat caataattgt aaacggataa gtaggacgaa taaatgtttt tcacggaaca
     2281 tatacaataa gataaattgt atggttcacg cgtataaaat atattaaatg aatataggag
     2341 gataaaatgt atagcttggg aattgatctg ggtggaacca atatagccgt agggttggta
     2401 aatgaggaag gtaaaattat acataaggac agtgttccta ccctcaggga aaggccgtat
     2461 cctttgatta tcaaggatat ggcgatgctt gcactgaaag taaccgagga tgccggtgtt
     2521 agcattgaca ggatttcagg gataggagtt ggaagccctg gaacaccaaa ttgtaaggaa
     2581 ggcattcttg tttacaacaa caatttgaat ttccgtaatg ttcctataag gtcagaaatt
     2641 caaaagtata taaatttacc ggtttatctt gacaatgatg caaattgtgc agcattggca
     2701 gagagtgttg ccggagctgc aaagggtgca caaatttcgg ttaccattac tctgggcaca
     2761 ggtattggcg gaggagttgt aattgacggg aagatttaca gcggctttaa ctttgcggga
     2821 ggagaactcg gacataccgt tataatggtg gacggcgagc agtgttcttg cggaagaaag
     2881 ggctgctggg aagcttatgc ttccgcaact gcactcataa ggcagactaa gaaagcagca
     2941 caggagaatc cgaattccct tataaataag cttattgacg gagatctctc caaaatcgat
     3001 gccaaaactg cttttgatgc ggcaaaacag ggtgacagga ccggtgaatt ggtggttgaa
     3061 cagtatataa aatacatttc agagggacta ataaacatga tcaatatttt catgcctgag
     3121 gtattggtaa taggcggagg agtatgtaag gagggcgaat accttctcaa gcccttaagg
     3181 gagctgataa aacagggcgt ttacagcaag gaggatatac ctcagacaca gctcaggaca
     3241 gcacagatgg gaaatgatgc cggaattgtg ggcgctgcaa tgcttgcaag ggaataaatt
     3301 gagaataaag tattatgaga aatataaagt tgactattga atatgacgga acgaattatc
     3361 acggttggca aacgcagaat aatgccagga ctgttcagga tgtcattgaa aaggcaatag
     3421 agggtttgac gggagaaaaa gttgctctta taggatcaag caggacagat tatggagttc
     3481 atgccttggg acaggtagca aattttgcaa caaactcaac catcccgggc gataggtttt
     3541 gttatgccct caacaggata cttcccgaag atattgtaat acgggaatcg tgtgaagttg
     3601 gtttggactt tcatgcgagg tttcaggcaa agggtaaaaa atacaggtat attatataca
     3661 actccagatt cccgtcggca atactaagga accgagctta ccatgtttca tacaatctgg
     3721 acattgatga gatgaaaaag gctgcattaa gctttctggg aactcatgac ttctctacat
     3781 tcaaggccag cggcagcagc gttaaaacat cggttcgaac catcacaagt gtttcccttg
     3841 aaaagaatgg tgatgtgttg gagtttgaga tatgcggaaa cggttttttg tacaatatgg
     3901 tgcgaattat tgtgggtacc ttggtggatg tcggaatggg aaggataaaa gcagatgaca
     3961 tatcggatat aataaacggc aaagacagga aaaaagccgg acgcacagct ccggcacatg
     4021 ggttatacct tgtggaagtg tactattaga tgggtgctaa atgaagaaaa aatatggagg
     4081 gaacaaaact ggtgtttctg aaatgttttt tcttcatttt tatcaatgca cttatttagt
     4141 gggatttcac gaagtgaaat gaccaaaaag cgatgggggt tcgatgcccc
//
LOCUS       DAOOJC010000206         4177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16612_length_4177_cov_3.573751, whole genome shotgun sequence.
ACCESSION   DAOOJC010000206 DAOOJC010000000
VERSION     DAOOJC010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16612_length_4177_cov_3.573751"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1857)
                     /locus_tag="PLA01_06470"
     CDS             complement(<1..1857)
                     /locus_tag="PLA01_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD00979.1"
                     /translation="MNIDKLDIIAFGKLNNLILELSDGLNVICGANEAGKTTIQWFIR
                     GMLYSLKGGRSGRSGELPPLKRFKPWRGNDYMGSIAYSLDDGSHFTAERDFNESTVKV
                     YDSMYNDITASFEQSREKGPLFALKHLGINESCFERTVFIGQMDTKIDAAGSKELLDR
                     LSNIRESGAEDVSYKRAREALTEALTFHVGTERTTTRPLDVVNQKLDGLNLRRRGIIE
                     ERERLLEAYEELNRLLRLKKSLEERKQALVYANQAIDLRKNIESLKKQRKELSLILEE
                     TDKYEREKSLISERIEECNETIRRYEYYSRYINDDPAHMEILFGRLKEAEEKSKKLLG
                     KIAELREEIGDLASLPEEMMGLKSIDINDFIWYRLIKTGIFITASTAVLACIAAAFFP
                     SWVIKIAAIGVFVLLSSLSVYMWRRKAYYENLALRLNELKSSMDVLEKEISENEQYIN
                     EIKSSMVERLRACNIIAHDGDEVRKEHVETFKQELGMYGRALEDVELKKEKYGDIERY
                     LQSIYDRASSAYGIEFCDRDAIKKACYDISRKTDRMYEDIEVCTYRIKALYSTEELSK
                     EYDELSEKVLDADIINAQSYIDREINHVDSDINETMLEISKVRALTEAASSKE"
     gene            complement(1930..3138)
                     /locus_tag="PLA01_06475"
     CDS             complement(1930..3138)
                     /locus_tag="PLA01_06475"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HPD00980.1"
                     /translation="MRETKEGLETDFELEVTLGMNSLKFLHFADLHLDTPFSSLGDGL
                     KIQKQRRQDLYDVFDRIIDLAKREDADIILISGDLYEHYYSKNSSIHYINRKFEEIPG
                     IRVFIVPGNHDPYTQNSCYKNFEWSKNVFILSGNNPKIYIEDLNTCIYGAGFGCFHEK
                     DGVLEDIGSVDKKRINLMLIHGTLDMNFKENRYNPISSKELASYGMDYVALGHFHNRF
                     EKIAENTPVYNPGSPEPLGFDEEGEHGVFIGRIVFVSEKEKKLDVRFERISKRQYRTL
                     EIKSDCFSGDEQIIDMINSNFEGQEEGNNLINVILRGYTAQGYRVNVKNIEGILKDRF
                     FYVTIKDETVPRYDYEELMGDPGLMGLFVRKMLSMIENTNSEKEKHLLMKAMQYGVEA
                     LEQGKVEVLF"
     gene            complement(3107..3892)
                     /locus_tag="PLA01_06480"
     CDS             complement(3107..3892)
                     /locus_tag="PLA01_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00981.1"
                     /translation="MKIKLLLNSIFYYKNIGILVVLLGIGHMVREYFGIPAFYIACAQ
                     VVLYLGLVLISFTSKKFQDRFLQKMKQRNIKRLNNKCIKLASEATRYTNAAYYRKLSS
                     IMKDKDEVLRSYNKDKSNYLKGKITEQTLNLVVSYINLLKDFCIRFREASKVNINPIM
                     ERITQNNRRLNFINDPKVYEDIKRIIEMDERMIERHKEERSDLERVDARLEYIKSTIG
                     MLRHQINSGLDSAETAESVENILNEAVALENVLNERNKRRLRN"
     gene            complement(3949..>4177)
                     /locus_tag="PLA01_06485"
     CDS             complement(3949..>4177)
                     /locus_tag="PLA01_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD00982.1"
                     /translation="IARANAIESLGGDNTELKIKKLDLNSSRERARARAEAMLAGEQG
                     FEVKEKADSIDANENTDNLEVKEKTDNKVNS"
BASE COUNT         1103 a         1045 c          634 g         1395 t
ORIGIN      
        1 ttcctttgaa gaagcagcct cggttaaagc ccgcaccttg cttatctcta gcatggtttc
       61 atttatatcc gaatccacat ggttaatttc tctgtcaata tagctttgtg catttataat
      121 atccgcatcc agcacttttt ccgagagctc atcatattct tttgataatt cctctgtgct
      181 gtaaagggct ttaattctgt atgtacatac ttcaatgtcc tcatacatgc ggtccgtttt
      241 tctgcttata tcataacaag cttttttaat tgcatccctg tcacagaatt caattccata
      301 cgcagaagat gccctgtcat aaatggattg caaataacgc tctatatccc catacttctc
      361 ttttttaagc tccacatcct caagtgctct gccgtacatt ccaagctcct gcttgaaggt
      421 ttcaacatgc tcttttctta cctcatcccc atcgtgagca attatattgc acgccctaag
      481 cctttcaacc attgaagatt taatctcatt gatgtactgc tcattttcac ttatctcctt
      541 ttccagaacg tccatgctgc tttttaattc gttcagcctt agagccaggt tttcgtagta
      601 agcctttctc ctccacatgt aaacggataa agacgacaac aggacaaaaa cgccaatcgc
      661 tgcaattttt atcacccacg acgggaaaaa tgcagcagct atacaggcta agacggctgt
      721 agatgcagtg atgaaaatac ctgtttttat taatctatac caaataaaat catttatgtc
      781 aatacttttt aagcccatca tctcttccgg aagagatgcc aaatcaccaa tctcttccct
      841 caattctgca attttcccca aaagcttctt acttttctct tcagcctctt taagcctgcc
      901 gaacaaaatc tccatatgag caggatcatc gttaatatac cgggaataat actcataacg
      961 ccttatcgtt tcattacatt cttcaatgcg ttcggatata aggctttttt ccctctcgta
     1021 tttatctgtt tcttccaaga ttaaagatag ctcttttctt tgttttttta atgactctat
     1081 gtttttcctc aagtcaatag cttgatttgc ataaaccagt gcctgcttcc tctcctcaag
     1141 agactttttt aaacgaagga ggcggttcaa ttcttcataa gcctctaaaa gcctttctct
     1201 ttcctcaata atccctctcc ttctaagatt taacccgtcc agcttttgat ttacaacatc
     1261 aaggggccgc gttgtcgtcc tctctgttcc cacgtggaaa gtcaatgcct ccgtcaatgc
     1321 ttcccgggct cttttatacg aaacatcctc cgcaccgctc tcccgaatat tggaaagcct
     1381 gtccaaaagc tctttgctgc ctgcagcatc aatttttgtg tccatctgcc cgatgaaaac
     1441 agttctctca aaacaggatt cattaatgcc cagatgcttt agtgcaaata aaggcccttt
     1501 ctccctgctc tgttcgaagg aagcggttat atcattgtac atggaatcgt aaaccttcac
     1561 cgtactttca ttaaaatccc tctccgccgt aaaatgcgaa ccgtcatcca ggctgtatgc
     1621 aatgcttccc atgtaatcat taccacgcca gggcttgaac ctcttaaggg gtgggagctc
     1681 accactcctg ccgctccttc ctccctttag agaataaagc atacctctta taaaccactg
     1741 tatggtggtt tttcctgcct catttgcccc gcagatgaca ttaagaccgt cggagagctc
     1801 cagtattaag ttgttaagct ttccaaacgc tattatatca agcttgtcaa tattcatacc
     1861 ttctcctgga tggcattata ataaatataa taaccttact tctcagtgct ttatcctaat
     1921 ttcattccct tagaataata cctcgacctt gccctgttcc aaggcctcca caccatactg
     1981 catcgccttc ataagcaaat gtttttcctt ttcgctattg gtattttcta tcattgaaag
     2041 catctttctt acaaacagtc ccataaggcc cggatccccc ataagctcct catagtcata
     2101 ccggggaacg gtttcgtcct ttatggtcac atagaagaac ctgtctttta aaatgccctc
     2161 tatgtttttg acattaacgc ggtatccctg tgcggtatac ccccttaaaa tcacattaat
     2221 aaggttattg ccctcctcct gcccttcaaa gtttgagtta atcatatcta taatctgctc
     2281 atcacccgaa aaacagtcgg attttatttc caatgtacga tactgcctct ttgaaatcct
     2341 ttcaaacctt acatccagct tcttttcctt ttccgaaaca aagacaattc ttccgataaa
     2401 taccccatgc tctccctctt cgtcaaatcc cagaggttcc ggactccccg gattataaac
     2461 aggggtattt tcggcgattt tctcaaaacg gttgtgaaaa tgccccagtg ccacataatc
     2521 catgccatag gatgcaagct ccttgctgct tataggattg taccggtttt ctttaaaatt
     2581 catatccaaa gttccgtgaa tcaacatcag attaatgcgt ttcttatcaa cagaacctat
     2641 gtcttctaaa acaccgtctt tttcatgaaa acagccaaaa cctgcaccgt atatgcatgt
     2701 gttgagatcc tcaatatata ttttggggtt gttcccagaa agaataaaaa cgttcttact
     2761 ccactcaaaa ttcttgtagc aggagttttg ggtataggga tcatgatttc ccggcacaat
     2821 aaacaccctt atgccgggaa tctcttcaaa tttcctgttt atataatgaa tagatgagtt
     2881 ttttgaataa taatgctcat agaggtcacc gcttattaaa attatgtccg catcttccct
     2941 tttggcaaga tcgattattc tgtcaaaaac atcgtaaagg tcctgtctcc tctgcttttg
     3001 tatctttaat ccatcaccca aagaactaaa gggagtatca agatgcaaat cagcaaagtg
     3061 cagaaatttt aatgaattca tgcctaaagt cacctcaagc tcgaaatcag tttctaagcc
     3121 ttcttttgtt tctctcattc aaaacattct caagggctac agcctcattt aatatattct
     3181 caacgctttc tgcggtttct gccgaatcaa ggcctgagtt tatctgatgc cttagcatgc
     3241 ctatggtgct ctttatatat tccagccttg cgtccactct ctcaagatcg cttctctctt
     3301 ctttgtgtct ttcaatcatc ctctcatcca tctcaataat cctttttatg tcctcataca
     3361 ccttagggtc attgataaag ttaagcctcc gattgttttg agttatcctc tccatgatag
     3421 gattgatatt gaccttgctg gcctcccgga atcttataca aaagtccttg agcagattta
     3481 tatatgaaac caccaggttc aaagtctgtt cagttatttt gccctttaaa taattggatt
     3541 tatctttgtt ataggatctt aagacctcat ccttgtcctt cattatggaa ctaagcttcc
     3601 tgtagtatgc tgcattggta tacctggtag cttccgatgc caatttaata catttattgt
     3661 tcaacctttt tatattgcgt tgtttcatct tctgaaggaa acggtcctga aattttttac
     3721 ttgtaaagct tatgagcaca agtcccaaat acaggacaac ctgggcacag gcaatataaa
     3781 aagcaggaat accaaagtat tcccgaacca tatgtccaat tcccaataaa accaccaata
     3841 ttccaatgtt tttataataa aatattgaat ttaataaaag ctttatcttc atatctcacc
     3901 ttttgcccga atgttatttt gcagcatccg ccagtatacc ggacaatttt atgaatttac
     3961 cttattatcg gtcttctcct taacttcaag gttatctgta ttctcattag cgtcaatact
     4021 atctgccttc tcctttactt caaatccctg ctctccagca agcatggcct ctgctctggc
     4081 acgggccctc tctcttgatg aattcaaatc gagcttctta atcttgagct cggtattatc
     4141 cccaccgagg ctttcaatag cattagctct tgcaatt
//
LOCUS       DAOOJC010000207         4168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16660_length_4168_cov_3.436664, whole genome shotgun sequence.
ACCESSION   DAOOJC010000207 DAOOJC010000000
VERSION     DAOOJC010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16660_length_4168_cov_3.436664"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..942)
                     /locus_tag="PLA01_06490"
     CDS             complement(49..942)
                     /locus_tag="PLA01_06490"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPD00983.1"
                     /translation="MIFLTFNDKLFVITHILVLIQTVFTTTYILLKAKKAPVTYSLLV
                     YMFLLILWIFFRFPECMSTSTEELLITVRFTLFPIFFSGVSWFIFSLFYTEKITAKNK
                     KLIIFLVSPVAICYLPALTNRFFYLIIVRKTVEDPANTEWGLFFWIFYVINTVYVFGG
                     IFLVLKKSINQYNILKKQIIVLVFSVLIPCLANIIGFVKVCNFPFDITPLSFSISLTL
                     LSISIFKFKFLDIYPFAMKEVFNSMKESVIITDSYNNIIDYNRSFLDHFDKFVPVEKD
                     QNIKYLLQSLEQNTRSIMELE"
     gene            1250..>2727
                     /locus_tag="PLA01_06495"
     CDS             1250..>2727
                     /locus_tag="PLA01_06495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HPD00984.1"
                     /translation="MFKKMGRKVCVFALAGIMTLNIGMQTTFAYSNIPAKLSLENTAN
                     LLEKNADKEKEKYVKGELIVKYKSTKTDKGKVKDKIKKSLKKSLKKKSVVPQEKEELS
                     SVIDVLEVDENEDIDSLAKSISEDPDVEFAQPNYIYYASAIPQDERYSEQWSLKNPST
                     GFDINAEKAWDITRGSSNVVVAVLDTGIAIGHPDLAGNIYVNPDEIIDNDDNDGNDKD
                     DDLNGWDFVNDDNTVYDLNDKTHGTHVAGTIAASANNIGVSGVAPNVKILPLKVLGTD
                     GRGSTSGLVKAIEYAASKGAEIVNMSLGGPDYDPALIDIMNRNPQTLFVVAAGNDHCD
                     LSKVPTYPASHDLPNMLVVASVDPNGSMSSYSNYGDAVDVAAPGRDILSTVASYENEN
                     IEYSYGLLDGTSMAAPHVTGVAALVKSRYPDADAAEIINRIKGSASINEDIKNWVPGG
                     LMLDAYGSINNLGSIDFTVSKASENEIYLEWMPVPGAASYKCS"
     assembly_gap    2728..2737
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2738..>4168
                     /locus_tag="PLA01_06500"
     CDS             <2738..>4168
                     /locus_tag="PLA01_06500"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HPD00985.1"
                     /translation="SVGYDGSFITINTYLTAYSLEAGKTYAFRVAALDAWGNEIAESR
                     FMKSTLSEGTGTGLKANYFNDAGMNELKVKRNENIDFNWGSGSPDASINNENFSVIWN
                     GEIEPRYSEDYTLYVQSSGACKVWVDGNLIINRATDDSSMVELSGNLNLNAGEHYPIQ
                     VEYYETNGDASIKLLWSSASQDKEVVPASRLYETSIISGIWSRTDESEYGSAFNNTFA
                     MVEGGKLYVLSCEVLGDAYIEVYDELQNKFVRLTQMKAKLINGATAIVSPYFGATSLN
                     GKIYTFISWEDGLNDNAPYAAVQEYDIATGTVTNKNFVYGLALPLFDTAITVNGQIYV
                     FGGSNNSNVYVYDPEADTWTAVAQMPDNRDCYAIAQCDGKVYLMGGQIGTENLSSVYM
                     FDPQNNSWTRKADMIDPLTYGCAAEKNGKILVYGGYAGYTERSRFTRNGYAYDPANDE
                     WSTIPSLISPRGHFGMENLGGRIFAV"
BASE COUNT         1430 a          661 c          903 g         1164 t
ORIGIN      
        1 gtgaaaaaca gaatttaata tgatattatg aaaaaagcaa atacctatct attcaagttc
       61 cataatgctt ctagtgtttt gttccaacga ttgcaacagg tattttatgt tctggtcttt
      121 ttcaacaggt acgaacttgt caaaatggtc cagaaacgag cggttatagt caataatatt
      181 attatagctg tcagtaatta ttactgactc cttcatactg ttgaacactt ccttcattgc
      241 aaaaggatat atatccagaa atttgaactt gaatatcgat atggacagaa gtgtcagaga
      301 aattgaaaaa cttaaggggg taatatcaaa agggaaatta caaactttaa caaagcctat
      361 tatattagca aggcagggta ttaatactga aaacaccagc actattatct gcttttttag
      421 aatattgtat tggtttatag actttttaag tactaagaaa attcccccga aaacataaac
      481 agtatttatg acataaaata tccagaaaaa taaaccccat tcggtattgg cggggtcttc
      541 aacagttttt ctgacaataa tcaagtaaaa gaacctgttg gttaaagcgg gcagataaca
      601 gattgcaact ggagagacaa gaaagataat taatttctta ttttttgcag ttattttttc
      661 tgtataaaac agtgagaaaa taaaccagga aaccccgctg aaaaaaatag gaaaaagagt
      721 aaatctcacg gttatgagca gttcctccgt tgaggtggac atgcactcag ggaaacggaa
      781 aaaaatccac aggatcaaca aaaacatata tacaagcaaa ctataagtta caggtgcttt
      841 ttttgctttt aggagtatat aagtagttgt aaaaacggtt tgtatcaaaa ccaatatatg
      901 tgttataaca aacaatttgt cattaaaagt caaaaaaatc atacaatgtt ccccaataaa
      961 gatgtcactt tgcaatgtat aattgcttaa attttatcat atttttgtct aagtttaaat
     1021 tgatatcttt caaaaaatag ttactttggt aatatgaata ttactaaagt aacaaattgg
     1081 caataaatac cattactttc gttatcttga agacggtttg aaattaatgt aatctaaagg
     1141 caagataatc tttccagttg tgataaaacc cgggtcaaag ttaatttatc acaatcttaa
     1201 ttatctggta atggataagc ttataaaaat attaaaggag gcagctttta tgtttaaaaa
     1261 aatggggaga aaagtgtgcg tatttgcttt agccggtatt atgactttaa atataggtat
     1321 gcaaacaacc tttgcatact cgaacatccc ggcaaagctg agtcttgaaa acaccgcaaa
     1381 tctattggag aaaaatgcag acaaagaaaa ggaaaagtat gtgaagggtg agttgattgt
     1441 aaagtataag agtacaaaga ctgacaaagg aaaagttaaa gacaaaatta aaaaatcatt
     1501 gaaaaaatca ttgaaaaaga aatcggttgt gcctcaggag aaggaagagt taagcagtgt
     1561 aattgatgtt ttagaggttg atgaaaacga agatatagac agtctggcaa aaagcatcag
     1621 cgaggatcct gatgtggagt ttgcccagcc gaactatatc tattatgcct ctgccatacc
     1681 tcaggatgaa aggtattcgg aacagtggag cttaaaaaat ccatcaacag ggttcgacat
     1741 taatgccgaa aaggcctggg atattaccag aggaagcagc aatgttgtgg tagcagtttt
     1801 agatacggga atagcaatag ggcatccgga tcttgcaggc aatatatatg taaatcctga
     1861 tgagataatt gacaacgatg ataatgacgg aaatgataaa gatgacgact tgaacggttg
     1921 ggattttgtc aatgatgaca atactgttta tgacttgaat gataaaacac atggcacaca
     1981 cgttgcggga actatagctg caagcgctaa taatattggt gtgtcgggtg ttgcaccaaa
     2041 tgtaaaaata ctccctctta aagtacttgg tacagatggc agaggaagta catcaggttt
     2101 agtaaaggct atagaatatg cggcatcaaa aggtgctgaa atagtaaaca tgagccttgg
     2161 ggggccggat tatgacccgg cactgataga cataatgaat agaaatcctc aaacattatt
     2221 tgttgtagca gcgggaaatg atcactgtga cctgtctaaa gtacctacat accctgccag
     2281 tcatgatctt cccaatatgc tcgtggttgc cagtgttgac ccgaacggtt caatgtcaag
     2341 ctattcaaac tacggcgatg cagtagatgt tgcagcaccc gggagagata tattaagcac
     2401 tgtagcatcc tatgaaaatg agaatattga atacagttat ggattactcg atggtacttc
     2461 catggctgca cctcatgtaa cgggtgtggc ggcgcttgtc aagtccagat atccggatgc
     2521 ggacgcagca gaaattataa acaggataaa gggctcggct tcaataaatg aagatatcaa
     2581 gaactgggtt ccgggcggtt tgatgctgga tgcgtacggt tcgataaata accttggaag
     2641 tattgatttt actgtatcaa aagctagtga gaatgaaatt taccttgaat ggatgcctgt
     2701 accgggagcg gcttcataca agtgttcnnn nnnnnnngtt cagttggcta cgatggttct
     2761 tttattacaa tcaacacata tctgactgca tattcccttg aagcaggtaa gacatacgca
     2821 ttcagagtag cagcactgga tgcttggggc aatgaaattg ctgaaagcag gtttatgaaa
     2881 agtactctca gcgaaggaac aggaacaggt ttaaaagcca attattttaa cgatgcagga
     2941 atgaatgagc tgaaagtaaa aagaaatgaa aacattgatt tcaactgggg aagcggctca
     3001 ccggacgcgt caataaataa tgagaacttt tctgtaatct ggaatggtga aattgaaccc
     3061 aggtattccg aagattatac tttatatgtg caatcttcag gagcatgcaa agtatgggtt
     3121 gatggcaatc tgataataaa cagggcaacc gatgattctt caatggttga gttgtccgga
     3181 aatttgaatc ttaatgccgg agagcattat ccgatacagg tggagtatta cgaaaccaat
     3241 ggggacgcct ccattaagct gttatggtca agtgcttccc aggataaaga agttgttcct
     3301 gcatccagac tatatgagac atccattata agcggaattt ggtcaagaac agatgaatcg
     3361 gaatatggca gtgcatttaa caatacattt gccatggttg aaggcggtaa actgtatgtg
     3421 ctttcgtgcg aagttttagg tgatgcctat atagaagtct atgatgaact ccagaataaa
     3481 tttgtaagat taacacaaat gaaagcaaaa ctcataaatg gcgctactgc gattgtcagt
     3541 ccttactttg gagctacgtc gttgaacgga aaaatataca catttatatc ttgggaagac
     3601 ggattaaatg ataatgctcc ttatgcagcg gttcaggagt atgatattgc aaccggcaca
     3661 gtgacaaata aaaacttcgt ctatggtttg gctttaccgt tgtttgatac cgcaattact
     3721 gtaaatggtc aaatatatgt ttttggaggc agcaacaata gtaatgtgta tgtatacgat
     3781 ccggaagcgg atacatggac tgctgtggct caaatgcccg ataacagaga ttgttatgca
     3841 atagctcaat gtgacggtaa agtgtatttg atgggtggac aaattggcac agaaaattta
     3901 agcagtgttt acatgtttga cccacagaac aattcatgga cccggaaagc tgatatgatt
     3961 gatcctttaa cgtatggctg cgcggctgaa aagaatggaa aaattcttgt ttacggtggt
     4021 tatgctggtt atactgaaag aagcaggttt acaagaaatg gatatgcata tgaccctgca
     4081 aatgatgaat ggtcaaccat tccaagtcta atttctccaa gaggacactt tggaatggag
     4141 aaccttggcg gaagaatctt tgcagtgt
//
LOCUS       DAOOJC010000208         4154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16726_length_4154_cov_3.669676, whole genome shotgun sequence.
ACCESSION   DAOOJC010000208 DAOOJC010000000
VERSION     DAOOJC010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16726_length_4154_cov_3.669676"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1269)
                     /gene="iadA"
                     /locus_tag="PLA01_06505"
     CDS             complement(106..1269)
                     /gene="iadA"
                     /locus_tag="PLA01_06505"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HPD00986.1"
                     /translation="MGGKLFVLLKNGICYNPELIGKKDILMVKNSIYKIEDNIDDKAF
                     CDIMTVDCSDKIICPGFIDQHIHIIGGGGEGGPASRIPEIMLSEIVSAGITTVVGVLG
                     FDSITRSLKGLLAKARALEEEGLNTFIYTGSYEVPTPTITGSVMSDVSLIDKVVGVGE
                     IAISDHRSSHPSQDSLKKIANEARIGGLLGKKAGVVHIHVGDGKDGLEPLNELAENSD
                     FPLDMFVPTHLNRNKPLFYQALEFAKRGGNIDFTAGQDTGTGYTVPDALKVCIDSGIG
                     LERVTVSSDANGSVPGNSADESGVGKAVQLFDDIRNSILNNKLDMSEVLKTVTVNVAK
                     ILKIYPVKGTIKQGSDADVLVLNKNSLDIDKVFINGEQFVEKGMVLRKGMYEK"
     gene            1614..2258
                     /gene="lexA"
                     /locus_tag="PLA01_06510"
     CDS             1614..2258
                     /gene="lexA"
                     /locus_tag="PLA01_06510"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPD00987.1"
                     /translation="MVKVSSDFNFLTSKQKKVYSVIEQFIKQRGIPPTVREIGEMVGE
                     KTPGAVQGILNRLEQKGVIKREVGMARSIQLVSDNSQYESHVYLPKIKKVTQRNVDDL
                     LNIYNIEKYFPLPSTLFEEGQDCLIIKCPDNSLFESGVKYDDILIIDRQQEIKDGDII
                     LVLYEGHTLLRFYSKHENPDFVVLKADSNLLEKETFNRSEVTIIGKLVGKYTQY"
     gene            complement(2472..3560)
                     /gene="leuB"
                     /locus_tag="PLA01_06515"
     CDS             complement(2472..3560)
                     /gene="leuB"
                     /locus_tag="PLA01_06515"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPD00988.1"
                     /translation="MAGYNIAVLPGDGIGPEVIEQAVQVINAVGEKFGHGFVIKEGLL
                     GGCAIDSTGEPLPQKTLDLCKSCDAVLLGAVGGEKWDSLPGNKRPEAGLLGIRAGLGL
                     YANLRPAVIYSALKGASPLRPDIVEDGVDILVVRELTGGIYFGDKGRVQTEKMGEAAY
                     DTEKYSVYEVERIARLAFETAMKRKKKLMSVDKANVLESSRLWRAVVTRVAKDYPQVE
                     LSHMYVDNAAMQLVRNPRQFDVIVTSNMFGDILSDEASMITGSIGMLPSASLGEGTMG
                     LYEPIHGSAPDIAGQDKANPIATILSVAMMMKYSFGLDAVSDTIEDAVVRVLEKGCRT
                     ADISSPQTTHVVGTKEMGRLIISEIKDN"
     gene            complement(3748..>4154)
                     /locus_tag="PLA01_06520"
     CDS             complement(3748..>4154)
                     /locus_tag="PLA01_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPD00989.1"
                     /translation="LLVMELFVLISRALKMEWESSIKNKSPKIKELIQVSVSYIHNNY
                     ERDISLKDIAQYVFLSTSYFTRAFKEEMGISPISYLLKLRIERAKELLKETDKRISDI
                     ALSVGFSNQQRFNDIFKKYVKLTPLQYRKKQD"
BASE COUNT         1223 a          940 c          679 g         1312 t
ORIGIN      
        1 attcatattc ataaagattt cacatttatt taagtgaaga aaaatgtgga aggaatattt
       61 ttctttacat ttatttaggt gaagaaaaaa atggagggaa ccaacttact tttcgtacat
      121 gcctttcctc aacaccatcc ccttttcaac aaactgctca ccgttaataa aaaccttgtc
      181 aatatccagg ctgtttttat tcaacacaag cacatccgca tcactgcctt gctttatagt
      241 gcctttcacc ggatatattt taagaatttt tgcaacgttc accgtaacgg ttttcaacac
      301 ctcagacatg tccaatttat tatttaaaat gctgtttctt atatcatcaa aaagctgtac
      361 tgcctttcct actcccgatt catcggcact attcccggga acactgccgt tcgcatccga
      421 tgatacggta acccgctcca accctatacc tgaatcaata cataccttca gtgcatccgg
      481 aacagtatac cccgtgcctg tatcttgacc tgccgtaaaa tcgatatttc ctcccctttt
      541 cgcaaactcc aaagcctggt aaaacagggg cttgtttctg ttcaagtggg tgggtacaaa
      601 catatccaaa ggaaaatccg aattctctgc caactcattg agcggttcaa gaccgtcttt
      661 tccatccccc acatgtatat gaacaactcc agcctttttc cccagtaatc ccccaattct
      721 tgcctcgtta gctatttttt taaggctatc ctgtgacgga tgggaagacc tgtgatcgga
      781 gatggctatt tcccccactc caacaacctt gtcaatcagt gatacatcgg acatgacact
      841 gccggtaata gttggagtcg gaacctcgta gcttcccgta tatataaagg tgtttagtcc
      901 ctcttcctcc aatgccctcg cctttgccaa gagtccttta agacttcttg ttatgctgtc
      961 aaaccccaaa acaccaacaa ctgttgtgat tccggcactg acaatttcag acagcataat
     1021 ctccggtatt ctgcttgccg gtcccccttc tcctcctccg ccaataatgt ggatgtgctg
     1081 gtcaataaaa ccgggacata ttattttgtc actgcaatct accgtcataa tatcacaaaa
     1141 ggctttatca tctatgttat cttcaatttt atagattgaa tttttaacca tcaatatgtc
     1201 tttctttccg ataagctcag gattatagca aataccgttt ttcaacagca caaacagttt
     1261 accgcccatg ttaatatatt cctttcgaaa aatccaagca caaaaagtat ttaaaggaga
     1321 tctaataaaa aatataatgt gcagttatat ataaaaaata ctgcaagatt ccgaaaattt
     1381 aagaattttg attaattttt tgtgaattta cgaacagttg tatgctgcta tttacgtatt
     1441 attaacagta ttactattaa ttaaagttga tatattgcat tttatttgct aaactaattt
     1501 taatgtttta tgtaaatcaa atttaaacat tgttttttta atttaaatgt tataggatat
     1561 aaaaaaagtc tgctgcctta ttaatgcaga ctactaaata taaatgtaaa aggatggtta
     1621 aagtgtcttc agattttaat ttcttaacct caaagcaaaa aaaagtttat tcagtaatcg
     1681 aacaattcat caagcaaagg ggtatacctc ctacagtcag ggaaattgga gaaatggtgg
     1741 gagaaaagac cccaggtgcg gtacagggta tactgaatcg tttggaacaa aagggtgtca
     1801 ttaagagaga agttggtatg gcaagatcca tacagcttgt ttcagacaac tctcaatacg
     1861 aatctcatgt atatttaccg aaaatcaaga aagtaaccca acgaaacgtg gatgatctgc
     1921 ttaacattta caacatcgag aaatatttcc cgcttccttc aactcttttc gaagaaggcc
     1981 aggactgttt aataatcaag tgtcctgaca acagtctttt cgaaagtgga gtaaagtacg
     2041 atgatattct cataatagac agacagcaag agatcaaaga cggagatatc attctggtgt
     2101 tgtatgaagg ccacacttta ttaaggtttt actccaagca tgaaaatcct gatttcgttg
     2161 tcttaaaagc cgacagtaac ttacttgaaa aagaaacttt caacaggagc gaagttacaa
     2221 taatcggaaa gcttgtaggt aaatatactc aatactaata gtctgataaa gaatatatcc
     2281 agctaaagct gaatactggg ttttcaatgg gggatccaat cccccattga agtcagaaag
     2341 agaacaatga gttttcaata aatgaaaaaa agtatcatat gttgaaatct cttaggtttt
     2401 gcattctttt tgttgccaaa ggtcccctat attctccacc ttttgcaata ttgcctttgt
     2461 atcggaaaaa gctagttgtc ctttatttcc gatattataa gtctgcccat ttcctttgtc
     2521 ccaacaacat gggtggtttg cggtgaagaa atgtctgctg tcctgcagcc cttctcaaga
     2581 actcttacaa ctgcatcttc tatggtatct gagacagcat caagaccaaa ggaatatttc
     2641 atcatcatcg ctaccgacag tattgttgcg atagggtttg ctttgtcctg ccctgctata
     2701 tcgggagcag aaccgtgtat gggctcatac agtcccatag tgccttctcc aaggctggct
     2761 gacggaagca ttcctataga accggtaatc attgatgcct catcggataa aatgtctcca
     2821 aacatgtttg atgtaactat gacatcaaat tgcctgggat ttctcacaag ctgcatagcc
     2881 gcattatcca catacatatg ggaaagctct acctgcggat agtccttggc taccctggtc
     2941 actaccgctc tccagagtct cgagctttca agtacattcg ccttatccac cgacataagt
     3001 tttttcttcc tcttcatggc tgtctcaaag gcgagtcttg caatcctttc aacttcataa
     3061 acagaatatt tttctgtatc ataggctgct tcacccattt tttcggtttg tactcttcct
     3121 ttatcaccga agtatattcc gccggttagt tccctcacca ccaaaatgtc cacaccatct
     3181 tccactatat ccggtctcaa aggcgatgca cccttcaatg cggaataaat taccgccggt
     3241 ctgaggtttg catagagacc caaacctgca cgaattccga gcaaacccgc ttcaggcctt
     3301 ttgtttccgg gaaggctatc ccacttttct cctccaacag ctcccaaaag aactgcatca
     3361 caagacttgc aaagatcaag tgtcttttgg ggaaggggtt ctcctgtcga atcaatggca
     3421 caccctccca gcaagccctc ctttataaca aacccgtgac cgaatttttc acccacagcg
     3481 ttaattacct gaaccgcctg ttcaatcact tccggaccaa taccgtctcc cggcagcact
     3541 gctatattat atccagccat aacctacctc ctcattaact ataaataaaa acagtaacct
     3601 gcctttatcc gcacctttat ttcatttatc cgtatctgga aaagcacacg ttacttaata
     3661 taattaagat tataaactat actttattac tattcttcct tttatttatc ttatttgttc
     3721 cgataattta atgattattg acattcatta atcttgtttc tttctgtatt ggagtggtgt
     3781 aagctttaca tactttttaa agatatcatt aaatctttgc tggttggaaa atcccacact
     3841 taaggcaatg tcacttatcc ttttatccgt ctcttttaaa agttcttttg ctctttctat
     3901 tctaagcttt aacagatagc ttataggact aatgcccatt tcctctttaa acgccctggt
     3961 gaagtaactg gtgctcaaaa aaacgtactg tgcaatgtcc tttaaagaga tatccctctc
     4021 atagttatta tgtatataac ttaccgaaac ctgtataagc tcctttatct ttgggctttt
     4081 attttttatg ctgctttccc attccatttt cagtgcacgg gaaatcagca caaacagttc
     4141 cataaccaga aggt
//
LOCUS       DAOOJC010000209         4151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16738_length_4151_cov_3.827393, whole genome shotgun sequence.
ACCESSION   DAOOJC010000209 DAOOJC010000000
VERSION     DAOOJC010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16738_length_4151_cov_3.827393"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PLA01_06525"
     CDS             complement(<1..662)
                     /locus_tag="PLA01_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HPD00990.1"
                     /translation="MKKVVVYDSTLRDGAQAQGISFSVKDKLKIVERLDRLGIRYIEA
                     GNPGSNPKDLEFFDRVSRVKLRHAKVIAFGSTRRVNTVVHEDANVKSLLKADTPAVAI
                     FGKSWDFHVTDILNTTLDENLKMIFDTISFFKNKNKEVVFDAEHFFDGYRANPEYAMK
                     TIKAAVEAGADSICLCDTNGGSFPDEVYTITSKVVDEFKVDVGIHCHNDTGMAVANSI
                     MAV"
     gene            complement(698..2245)
                     /locus_tag="PLA01_06530"
     CDS             complement(698..2245)
                     /locus_tag="PLA01_06530"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPD00991.1"
                     /translation="MIERVKIFDTTLRDGEQTPGVNLNITEKLEIARQLEKLGVDVIE
                     AGFAIASPGDFEAVKAVSKEIKNVTVASLARAVEKDIDRAWEALREAESPRIHTFIAT
                     SDIHLKYKLKMTEEEVLERAIAMVRYAKKYCSDVEFSAEDASRTRPEFLVKVVEEVIK
                     AGATVVNIPDTVGYTTPTEFGNLIKILKEKVSNIDKVDISVHCHNDLGLAVANSLAAV
                     LNGAVQVECTINGLGERAGNTAMEELIMGIDTRKDFYGIGHRINTTQIYRTSKLITSL
                     TGVKVQPNKAIVGANAFAHESGIHQHGVLSERSTYEIMRPEKIGLSQNRMVLGKLSGR
                     HAFEERLKEMGYTSLTPEKIQKAFEKFKSLADKKKEILDRDIEALIEEKVSEVPEIFE
                     LDTFQITSGNKSVATSTVTLIRNKIAITEAATGDGPVDAAFNAIERAVGTSFTLEDYN
                     LRAVTEGKDALGEVTVRVSKDEKMFIGRGVSTDIIEASVKAYVNAINRAISEIGEDSL
                     KIVAVQG"
     gene            complement(2374..3369)
                     /gene="ilvC"
                     /locus_tag="PLA01_06535"
     CDS             complement(2374..3369)
                     /gene="ilvC"
                     /locus_tag="PLA01_06535"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPD00992.1"
                     /translation="MAKMYYDSDCNLGLLKGKTVAIIGYGSQGHAHAQNLKDSGVDVV
                     VGLAPGSASRQKAIDAGLRVEDTDKAAKAADVIMMLVPDQLQKAIYEESIKPNLEEGN
                     VLAFAHGFAIHFNMIVPPNTVDVIMIAPKGPGHTVRSQYQEGKGVPSLIAVYQDASGK
                     AKDYALAYASGIGAGRAGILETTFREETETDLFGEQAVLCGGVTELMKAGFETLVGAG
                     YQPEIAYFECIHEMKLIVDLINQGGFAYMRYSISDTAEYGDYITGKKIITEETRKAMR
                     DVLKDIQNGTFASRWITENNAGGRAEFLATRRKEAEHQLEKVGAELRKMMSWLKK"
     gene            complement(3377..3889)
                     /gene="ilvN"
                     /locus_tag="PLA01_06540"
     CDS             complement(3377..3889)
                     /gene="ilvN"
                     /locus_tag="PLA01_06540"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HPD00993.1"
                     /translation="MGKHTLSVLVENQAGVLSRIAGLFSRRGFNIDSLAVGVTENPEV
                     SRMTIVVDGDQYIVEQVTKQLNKLVDVIKIKELDDSESVHRELVLIKVEATASTRSEI
                     MQIVEIFRANIIDVSKNTLTIEISGDTQKIDALENMLKQFGIKEVVRTGTIAIERGSK
                     IIKIKNSDEE"
     gene            complement(3948..>4151)
                     /locus_tag="PLA01_06545"
     CDS             complement(3948..>4151)
                     /locus_tag="PLA01_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPD00994.1"
                     /translation="QKLRYDCRYSQVFLDDNPDFIKLFDAYGFKGRRIDDNSLVEEAL
                     KEMLSDDGAYLLECRVDPEESTL"
BASE COUNT         1004 a         1128 c          719 g         1300 t
ORIGIN      
        1 actgccataa tagagtttgc cactgccatt cccgtgtcgt tgtgacaatg gattcccaca
       61 tccaccttaa attcgtcaac caccttcgat gtaattgtat atacctcatc agggaaggaa
      121 cccccgttgg tgtcacaaag acaaatactg tcggcaccgg cttcaacagc cgccttgatg
      181 gtcttcatag catattcggg attggccctg tagccgtcaa aaaaatgctc cgcatcaaac
      241 actacttcct tgttcttatt cttgaaaaag gatatggtgt cgaatatcat cttaaggttc
      301 tcgtctaatg tagtgtttaa aatgtctgtg acatgaaagt cccagctttt cccgaatata
      361 gccacggccg gagtatccgc cttgagcagc gactttacgt tggcatcctc atggacaacc
      421 gtattcactc ttctggtact tccaaaggca atcacctttg catgcctgag cttaaccctg
      481 gaaacccggt cgaagaactc caggtccttg gggttggaac cgggattccc ggcttcaata
      541 tatctgatac caagcctgtc aagcctctca acaattttca atttatcctt aacactaaaa
      601 gaaataccct gtgcctgggc accgtctcta agtgttgagt catataccac taccttcttc
      661 atctcaattc gtccttcctc acaatatctt tacttttcta cccttgtacc gccactatct
      721 tcaaggaatc ctcaccgatt tcgcttattg ccctgttaat ggcattcaca tatgctttta
      781 cactggcttc aattatatct gttgaaacac ccctgccgat aaacatcttt tcgtctttgg
      841 aaacccttac tgtaacttca cctaatgcat cctttccttc ggttaccgct ctcaaattat
      901 agtcctccaa agtaaagctt gttcccaccg ctctttctat tgcattgaat gcggcatcca
      961 caggtccgtc tccagtcgca gcctcggtaa tagctatttt atttcttatg agagtaacgg
     1021 ttgaggtcgc aacactctta ttaccgctgg taatctggaa ggtatcaagt tcgaaaatct
     1081 ccggaacttc cgaaaccttt tcttctatca aagcttcaat atccctgtcg agaatttcct
     1141 tctttttgtc tgcaaggctc ttgaactttt cgaaagcctt ttgaattttc tccggggtca
     1201 gtgaagtata tcccatttct ttgagccttt cttcaaaagc atgccttccc gaaagcttcc
     1261 ccaacaccat tctgttctgg gaaagaccga ttttctccgg cctcataatc tcataagtgc
     1321 ttctttcgga cagaacaccg tgctggtgaa tacccgactc atgggcaaaa gcgtttgcac
     1381 ctactatggc cttgttgggc tgtaccttta cacctgtaag gctggttata agcttgctgg
     1441 tcctgtagat ttgcgttgta ttgatcctgt gacctatacc gtaaaaatcc ttccgggtat
     1501 cgatacccat tatcagctct tccattgcgg tattccccgc tctttcccca aggccgttta
     1561 tagtacactc cacctgaacc gctccgttga gtactgctgc aagggaattt gccactgcca
     1621 ggcccaaatc gttatgacag tgtacgctga tatccacctt gtcaatattg ctgacctttt
     1681 ccttcaggat ctttatcaga tttccgaact ccgtgggagt tgtgtagccc acggtgtcgg
     1741 gaatgtttac aaccgtagca ccggccttta ttacctcttc aacaacctta accaaaaact
     1801 ccggccgtgt tctgctggcg tcctctgcgg aaaactccac atcggaacaa tacttctttg
     1861 catacctgac cattgcaata gctctttcca atacttcctc ttcggtcatc ttgagcttat
     1921 atttcaaatg tatatccgat gtggctataa acgtgtgaat tctggggctt tctgcttccc
     1981 ttaaagcttc ccatgccctg tcaatgtcct tttccacagc cctggcaagg cttgcaacgg
     2041 taacattctt tatttcttta gacactgctt ttactgcttc aaagtcgccg ggcgatgcaa
     2101 tggcgaaccc cgcctcaata acatccacgc ccagtttttc cagctgcctt gctatttcaa
     2161 gcttttcggt aatatttaaa ttaactcccg gagtctgttc tccgtccctt aatgtcgtat
     2221 caaatatttt aactctttca atcatttcga atcctccttt acgaatcaat cccatattta
     2281 taaatatcta aataaaataa tgtttgttat ttcccaaccc tccccgaaac ggggaaggtc
     2341 aggaatatta ttatgtatct tttgtttaat accttatttt ttaagccagc tcatcatctt
     2401 tctgagctcc gcacccacct tctccagctg gtgctccgct tcctttcttc tggttgcaag
     2461 gaactctgct ctgccgccgg cattgttctc ggttatccac cttgaagcaa aggtaccgtt
     2521 ctgaatatcc ttaagtacat ctctcattgc cttccttgtt tcttcggtga ttatcttctt
     2581 tcctgtaata taatcaccat actccgctgt atcgcttatg gagtatctca tgtatgcaaa
     2641 gccgccctga tttataaggt caacaatgag cttcatttca tgaatacact caaaataagc
     2701 tatttccggc tgatatcctg cacccaccaa ggtctcgaat ccggccttca taagctcggt
     2761 aactcctccg cacaaaacgg cctgctcacc gaacaaatct gtctcggtct cttctctgaa
     2821 ggtggtttca agaattcctg ccctgcctgc acctatgccg gatgcatatg caagagcata
     2881 atccttggcc ttgccggaag catcctgata aacagcaatc agtgaaggta ctcctttgcc
     2941 ctcctgatac tggcttctta ccgtatgacc cgggcccttc ggtgcaatca ttataacgtc
     3001 aacggtattg ggaggaacaa tcatattgaa atggatggca aacccgtggg caaaagcaag
     3061 aacattgcct tcctccaggt tcggctttat gctctcttca taaattgcct tctgcaattg
     3121 atccggtaca agcatcatta ttacatccgc agccttagcc gccttgtctg tatcttccac
     3181 cctcagccct gcatctattg ccttttgcct tgatgcagac ccgggagcca aacctaccac
     3241 aacatccact ccactgtctt taaggttttg tgcgtgtgca tgtccctggc ttccatagcc
     3301 gataatagct actgtctttc ctttcaataa gcccaggttg caatcactgt cataatacat
     3361 cttcgccatt ttaatatcac tcctcatcac tattcttaat ttttataatt ttactgcctc
     3421 tttcaatggc tattgtaccc gttcttacaa cttcctttat accaaactgc ttgagcatat
     3481 tctccagagc gtcaattttt tgggtgtctc cggatatttc aatggtcagc gtgtttttag
     3541 acacatcaat tatatttgcc ctgaatattt ccactatctg cattatctcg gaccttgtgg
     3601 atgcggtggc ctcaaccttt atgagaacca actccctgtg gaccgattcc gaatcatcca
     3661 gctcctttat ctttatcacg tcaaccagct tgttaagctg tttggtaacc tgttccacaa
     3721 tgtattgatc cccgtcaaca acaattgtca ttcttgaaac ctcaggattt tcggtaacac
     3781 ccaccgcgag gctgtcaata ttaaatcctc ttctgctgaa caatccggct atccgggata
     3841 atactccggc ctgattctcc accagaaccg ataaagtgtg tttccccatt tttaaaacct
     3901 ccattaacaa gtatttataa caataaataa ttatctatat caagccacta tagcgttgac
     3961 tcctcggggt caaccctgca ctccaaaaga taagcaccgt catcggaaag catttccttt
     4021 aatgcttcct caaccaggga attgtcatct atccttctgc ccttaaagcc ataagcatca
     4081 aacagcttga taaagtcagg attatcgtcc aaaaatacct gggaataacg gcagtcatat
     4141 ctgagctttt g
//
LOCUS       DAOOJC010000210         4150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16741_length_4150_cov_3.830037, whole genome shotgun sequence.
ACCESSION   DAOOJC010000210 DAOOJC010000000
VERSION     DAOOJC010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16741_length_4150_cov_3.830037"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLA01_06550"
     CDS             <1..582
                     /locus_tag="PLA01_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPD00995.1"
                     /translation="KDERIVTIEDSAELQIVGLDNIVKMETRNANTEGKGEITIRDLI
                     KTSLRMRPERIIVGEVRGPEALDMLQAMNTGHDGSLSTGHANSPKDMLSRLETMVLSG
                     SAMPLEAIRQQIASAIDIIIHLARLRDKTRRTMEITEIVGYENGEIILNPLYEFEEEG
                     ETPQKVIIGSLKRTKNKMINTWKFKMAGIPGEF"
     gene            631..1422
                     /locus_tag="PLA01_06555"
     CDS             631..1422
                     /locus_tag="PLA01_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPD00996.1"
                     /translation="MGSVFKNSKPFEKADVLIDYDVYVMKPVEKILYVIAAAVVIFII
                     AFVFYHSIILSLLLTPLSLFYPKIRTKEIIRKRKNELNLQFKDLLYSLSSSMTAGKSI
                     ESSFKEAIKDLSILYPSPETYIIQEVEYIVRRIEMNETIESALEDLSRRSHLEDIENF
                     FDVFKTCKRTGGNMVSVMRNTSNIINDKIEIKEEINTMLSSKKFEQKVLSIMPVFIIA
                     LLSVTTEDYMAPVFNMPIGRIVMTIAMVVIAAGYFISAKIMDINV"
     gene            1440..2306
                     /locus_tag="PLA01_06560"
     CDS             1440..2306
                     /locus_tag="PLA01_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPD00997.1"
                     /translation="MIIVFLVYFIILIVLFLLSRNKYNDYIEPLDKKEYKLKSFLPMG
                     FFILDKIKYKYASKYDRMLQSKITELREAKYSLYYLQVHMANKVTLMLVVLLLFTFAG
                     ISMDEFDISYVVFGVLALGAVVYFTDNELTEKVKKRRLSIQIDFPDFLNKLILLVNAG
                     MAMPRAWEKIVTESKKDTVLYNELRTVLADIRGGKSQISAFEDFAKRCRIPEITKFVS
                     VALQNLSRGNAEMIPILRLQATECWEMRKHAAKRLGEEASTKMLFPMILMFVAIILIV
                     ATPAILALISSM"
     gene            2329..2517
                     /locus_tag="PLA01_06565"
     CDS             2329..2517
                     /locus_tag="PLA01_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp1 family type IVb pilin"
                     /protein_id="HPD00998.1"
                     /translation="MLSLIKNFIKEEDGMGTVEVVIIVAVLVAIALIFRKAIYSFVKE
                     IIGKIFTDGVKDDVTEGL"
     gene            2698..3402
                     /locus_tag="PLA01_06570"
     CDS             2698..3402
                     /locus_tag="PLA01_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HPD00999.1"
                     /translation="MMCFREGMIKENKGSFTVEACIIVPIVIISILALIMIGEFLYQQ
                     SCIQSIANRAAQRGAEIWNCPAKDMVYAQITKDRMKDVSLYWRIGESDSERQVKKSKI
                     EEYAKYMVFHDSVLGEPVNVEATANMVEDYIFYKKIRVTVTAKYNNPFAPFLRAFGLK
                     DTITIKAHSDAVINEPVEFIRSTDFTIDVVEEIDNKLLNNKGSEAIANIREGFANIFS
                     KVRGFIDGKAENNEGG"
     gene            3409..>4150
                     /locus_tag="PLA01_06575"
     CDS             3409..>4150
                     /locus_tag="PLA01_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014522643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HPD01000.1"
                     /translation="MRIIKKTEGAISIFLCIILLAMMVLASVLVEGTRVKSATTQIDS
                     ALDSSVKSALANYNYLLKELYGLMALSGDDPELLKDEIAYYLERTLMIADTKENDAES
                     ESLWNSINDSKTGSAVLDFFGFGDKGLPPLDLYDYGIEELKVQPLYNLSEPEVLRAQI
                     LEYMKYRAPKQLAEGLMDKFLTFKDFKKQSDVLSKKMDVDKKLTDIKEKQITASDNIV
                     TVNKFSTDFNIPNQLEIAAGYILDRIKIE"
BASE COUNT         1382 a          610 c          958 g         1200 t
ORIGIN      
        1 aaagatgaaa ggattgtaac catagaagat tctgcagaac ttcaaattgt cgggctggac
       61 aacatagtca agatggagac gagaaatgcc aatactgagg gaaaaggaga aataacaatc
      121 cgggatttaa taaagacatc ccttagaatg cggccggaaa ggataatcgt cggagaggtg
      181 cgtggacctg aagcattgga catgcttcag gcaatgaaca ccggacatga cggttctctt
      241 tcaacgggac acgcaaattc tccaaaggat atgctttcaa gacttgagac aatggtattg
      301 agcggttctg cgatgccttt agaagccata aggcagcaga tagcctcagc gatagacata
      361 attatacatc ttgcaagact tagggataaa acaaggagga ctatggaaat caccgagatt
      421 gtaggatatg aaaacggaga aataatttta aatccccttt acgagtttga agaagaggga
      481 gaaacaccgc aaaaggttat cattggctct ttgaagagaa caaaaaacaa gatgattaac
      541 acatggaaat ttaaaatggc gggcataccg ggtgaatttt aaggctgcca ggccagtggg
      601 acataaacca taaggaaaga ggaaggtaga atgggttcgg ttttcaaaaa ttcaaagcct
      661 tttgagaagg cggatgtttt aatcgattac gatgtatatg taatgaaacc ggttgaaaag
      721 attttatatg tcattgcagc tgctgtggtg attttcatca ttgcctttgt tttttatcac
      781 agcataatat tgtcgctgct tttgacacct ttgtctttat tctatccaaa gataaggacg
      841 aaggagataa taagaaagag aaaaaacgaa ttgaatcttc agtttaagga tcttttatac
      901 tccctttcct cttcaatgac tgccggaaag tccattgaat cttcgtttaa agaggctata
      961 aaggatttgt ccattttgta tccttctccc gaaacctata taattcagga agttgagtac
     1021 atagtgagac gaatcgaaat gaatgagact attgagagtg cattggagga tctttcaaga
     1081 agatctcatc ttgaggatat agaaaacttt tttgacgttt ttaaaacatg caaaaggaca
     1141 gggggaaaca tggtgagtgt tatgcggaat acttccaata tcattaatga caagattgag
     1201 attaaagaag aaataaacac aatgctgtca tcaaaaaagt ttgaacagaa ggttttatcc
     1261 ataatgcctg tttttataat agctttactt tcggtcacta ctgaggacta tatggctccg
     1321 gtatttaaca tgcctattgg caggattgtt atgacaattg caatggttgt aattgctgca
     1381 ggctatttta tttccgcgaa aattatggat attaatgtgt agcttttgtg gagggtgtta
     1441 tgattatagt atttttggtg tattttataa ttttgatagt gttgttttta ctgtcaagaa
     1501 acaaatataa cgactatatt gagcctttag acaagaagga atacaaactg aaaagctttt
     1561 tgccgatggg gttctttatt cttgataaaa ttaaatacaa gtatgcttcc aaatatgaca
     1621 ggatgctgca gtccaaaata acggagttaa gagaagcaaa gtattcgctt tattatctgc
     1681 aggttcatat ggccaataag gtcaccctta tgctggtggt actgctgctg tttacctttg
     1741 cgggtatttc catggatgaa tttgatataa gctatgtagt gttcggagtt ttagcacttg
     1801 gggcggttgt ttattttact gataatgagc ttacagagaa agttaaaaaa agaaggctta
     1861 gtattcaaat tgattttcct gattttctaa acaaactcat cctgctggtc aatgccggaa
     1921 tggcaatgcc cagggcatgg gaaaagattg taactgagag caaaaaggat actgttttat
     1981 ataacgagct cagaacggta cttgcggaca tcagaggagg caaatctcaa ataagtgctt
     2041 ttgaggattt tgccaagaga tgtaggatac cggaaataac caagtttgta tctgttgcat
     2101 tgcagaacct cagcaggggg aatgctgaaa tgataccaat actaaggctt caggcaacgg
     2161 aatgctggga gatgagaaag catgcggcaa agagactggg ggaggaggca tcaactaaaa
     2221 tgctgtttcc gatgatactt atgtttgtag caataattct gattgtggca actcctgcaa
     2281 ttttagcttt gataagcagt atgtaatttt tgaagggagg tgaagtattt gcttagtttg
     2341 ataaagaatt ttattaaaga ggaagacggt atgggaacag ttgaagtagt aataatagtt
     2401 gctgttttgg ttgcaattgc tcttattttc agaaaagcaa tatatagctt tgtaaaggaa
     2461 ataataggta agatatttac tgatggtgtt aaggatgatg ttacagaagg actatagtag
     2521 gacgcataac ggaatttgtt attagggcat atgaaaatgt gccctaatag atctttttaa
     2581 gtaattgtga atgaaaatgt tccggaaact ttagtttttt aaagtacaga gtcttttaat
     2641 actccacatt tttattcact tggaaaaagg atactcaatg gtagaggtac attttatatg
     2701 atgtgtttta gagagggaat gataaaggag aataagggaa gcttcacggt ggaggcctgt
     2761 ataattgttc cgattgtgat aataagcatt cttgcattaa ttatgatagg ggaattccta
     2821 tatcagcagt cctgtattca aagtattgcc aacagggcag cacagcgggg agctgaaata
     2881 tggaactgtc ctgccaagga tatggtttat gcacagatta caaaggatag gatgaaagat
     2941 gtgagtcttt attggaggat aggagagtcg gactcagaaa gacaagtaaa aaaatctaaa
     3001 atagaagaat acgcaaagta tatggtattt cacgattctg ttttaggtga acctgttaat
     3061 gtggaagcaa ccgccaacat ggtggaggac tatatatttt ataaaaaaat aagggttacg
     3121 gttacagcaa agtataacaa tccttttgca ccttttttaa gagcctttgg gcttaaggat
     3181 actattacaa taaaagccca ttcggatgct gtaattaacg agccggttga atttatcaga
     3241 tctaccgatt ttaccatcga tgttgtggaa gagatagaca acaagctttt aaataacaag
     3301 ggaagcgaag caatagccaa tattagagaa ggatttgcaa atattttttc caaggtcaga
     3361 ggcttcattg acggtaaggc tgaaaacaat gaagggggct gatgtattat gagaatcata
     3421 aaaaaaaccg agggtgccat atcaattttt ctttgtataa tacttttagc aatgatggtg
     3481 ctggcaagtg ttctggtgga agggacacgt gtaaagtcag caacgacaca aatagatagt
     3541 gctttggaca gttcggtaaa atcagcattg gcaaactata actatttact gaaagagctt
     3601 tatggactca tggctctatc cggggacgac ccggaacttc ttaaagatga aattgcatac
     3661 taccttgaga ggactcttat gatagcagat accaaagaaa acgatgcgga aagtgagtcc
     3721 ttatggaatt caataaatga ttcaaaaaca gggagtgcgg ttttggactt tttcggtttt
     3781 ggcgataaag gtttgccccc attggatttg tatgactatg ggattgagga attaaaggtt
     3841 cagcccctct ataatttgtc agagcctgag gttctgaggg ctcagatact ggagtatatg
     3901 aaatataggg ctccgaagca gctggcggaa gggcttatgg ataaatttct tacttttaag
     3961 gattttaaaa agcagtcgga tgtgttatca aagaaaatgg atgttgacaa aaaactgacc
     4021 gacataaagg aaaaacaaat cacagcatcg gataatattg tcacggtaaa caagttttct
     4081 accgacttta acatacccaa ccaactggaa atagcagccg gatatatatt ggacagaata
     4141 aaaatcgaaa
//
LOCUS       DAOOJC010000211         4128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16839_length_4128_cov_3.492021, whole genome shotgun sequence.
ACCESSION   DAOOJC010000211 DAOOJC010000000
VERSION     DAOOJC010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16839_length_4128_cov_3.492021"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLA01_06580"
     CDS             complement(<1..185)
                     /locus_tag="PLA01_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01001.1"
                     /translation="MVINEKSPYSPSNRETTKKVGSNNEVFDNSKDVSAPASGGELKT
                     NAVSTDKCTTAGDPVNVA"
     gene            363..878
                     /locus_tag="PLA01_06585"
     CDS             363..878
                     /locus_tag="PLA01_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPD01002.1"
                     /translation="MKSFEELCQENYKRIYKYIFSMTGNREAAEDLIQEVFTIAYEKG
                     ETFLQHEKPVAFLYRTARNISLTHLKRQRVFATEYLSENIAKDDTDLCDKLLTESDRN
                     IDETIYTGQVIDSLDSSKNELYKQRYVEHKSIREIASEQGTSETALRMRLVRLRREIQ
                     NIVKKLKLGEK"
     gene            957..2015
                     /locus_tag="PLA01_06590"
     CDS             957..2015
                     /locus_tag="PLA01_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01003.1"
                     /translation="MDNISELKKELEKAVLRNDIEKAEEISDKLFLLQGGREEYTVMP
                     TRFVEDIKEGNKQKTGGKKSMNMKKIISIFAAAAVITTIGITALATRWYGIRDLVFKN
                     NNKPAVTANTGSPEDTEAANMGNGQEEPDLIVMQGYPDSNEYKASAEWNLFCDGYDTD
                     GSLLSQVGNGSNEYTEKYPMYLVYTKEMADKLEEIVQKYGLSLHESITIVYDKEELTK
                     EAGTGDFIGDENTVLSGYVYNDGTFQYDGRAGLKNNKSIDYQFGNYVKGTFADTCLNI
                     GNADDYTEWEYKTKSGVRVSLALGNSKALVIADLPNSFITINVLSGAQGGLFSDSDTI
                     TAEVLQNFADSFDFSKIK"
     gene            2318..3328
                     /locus_tag="PLA01_06595"
     CDS             2318..3328
                     /locus_tag="PLA01_06595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD01004.1"
                     /translation="MQENENKSHRSQITSDDNKKTTECISIAVPCYNEEKTLQAMYNT
                     LCEIAASMREVDFEFIFVDDGSKDKTLEMMREIASKDSRVVYISFSRNFGKEAAMYAG
                     MQKAKGDYVAIMDADLQDPPALLPEMYRVIKEEGYDCAAARRVNRIGEPKIRSFFARR
                     FYKIMRKLSNMEVVDGARDFRLMEREMVNAVLSVCEYNRFSKGIFSWVGFRTKWLEYK
                     NVERIGGNSKWSFWKLFLYSIDGIVAFSTAPLALSSLLGICFCIIAFLAIIFTIVRQL
                     VWGGSAYGWSSLVCIILLLSGIQLFCIGILGQYLSKTYLEAKKRPIFIVREQKGGHNE
                     KE"
     gene            3315..>4128
                     /locus_tag="PLA01_06600"
     CDS             3315..>4128
                     /locus_tag="PLA01_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPD01005.1"
                     /translation="MKKNDNRKIIIAQLCFWIPALIMLAAFAKMGMAPFGKSSTMIMD
                     MSDQYVEFLCGLKYGDIYYSWSKTLGGNYIGIFAYYVSSPLSVFTLFWPNSQMPIAVL
                     FLTVIKIGLAGFTFSLFLKYRFRRYDLSTVLFSIMYALMAYNVVYSMCIMWLDAVIWL
                     PVILIGVEKVLKGKSRKLLIFALFVSFISTYYISYMTGLFTALYFLYRCVEKKLWSKK
                     LLTRVWLFASSVFLAASLGAFFLLPTLESLLSGELGSSGMVYSSTFNFKFVQF"
BASE COUNT         1243 a          708 c          953 g         1224 t
ORIGIN      
        1 gcaacattta ccgggtctcc cgccgttgta catttgtcgg tggaaacagc attggtctta
       61 agctctccac cgcttgcggg agccgataca tccttgctat tatcaaaaac ttcgttattg
      121 gaaccaactt tttttgttgt ttctctgtta ctgggcgaat aaggactttt ctcatttatg
      181 accatactta tctcccccct tttaataata tattctacac attaacccat acttttacaa
      241 taccgaatta atatgttaag gaggagatat cgtatgattc ctttaaatac cacattattc
      301 aagtgtaaaa attcttgtta cattattcga aacagtatat aaatataata gaggtgggca
      361 aaatgaaaag ttttgaggag ctttgtcagg aaaattataa aagaatttac aaatatatat
      421 tttctatgac cggaaacagg gaagctgccg aggatttaat ccaggaagtt ttcacaattg
      481 catatgaaaa gggtgagacc tttttacagc atgaaaagcc ggttgcattt ctgtacagga
      541 cggcacggaa catatcgctt acgcatttga agcgtcaaag ggtttttgca acggaatacc
      601 tgtctgaaaa tattgctaaa gacgatacgg atttatgtga caaactctta acggaatcgg
      661 accgaaacat agatgagaca atttataccg gtcaagtaat cgacagcttg gattcaagca
      721 agaatgagct ttataaacag cgttatgttg agcacaagtc cataagagaa attgcttcgg
      781 aacagggcac cagtgaaacg gcattgcgta tgcggctggt aaggctcaga agggaaatac
      841 agaacattgt caaaaaattg aaactgggcg aaaagtaata gatttgagtt acattaaacg
      901 aattttgata tatataagtg taaagtgctt tacagagtaa ctgggggttg tttattatgg
      961 ataatatttc agaattgaaa aaggaattgg aaaaggctgt tctgcgtaat gatatcgaaa
     1021 aagcggagga aatctccgac aaattgtttc ttttgcaggg cggcagggaa gagtataccg
     1081 taatgccgac ccgctttgtt gaagacatca aagaaggaaa taaacaaaaa acagggggta
     1141 aaaaatccat gaatatgaag aaaatcataa gtatatttgc tgctgcggct gttataacaa
     1201 ctattggcat tacagcattg gctacacgtt ggtacggcat aagggatctg gttttcaaaa
     1261 acaataataa acctgctgtt acggcaaata cgggttcacc ggaggatacg gaagccgcca
     1321 atatgggaaa tggacaggaa gaaccggact tgattgtaat gcagggatat cccgacagta
     1381 atgaatataa agccagtgcc gaatggaatc tgttttgtga cggatacgat actgacggca
     1441 gccttttaag tcaggtaggg aacggttcca atgaatatac ggaaaaatat ccgatgtatc
     1501 tggtatatac aaaggaaatg gccgataaac tggaggaaat cgtacaaaaa tacggtcttt
     1561 cattgcatga atccataaca attgtctatg acaaagagga attgacaaaa gaggcaggca
     1621 ccggtgactt tattggagat gagaatactg tcttaagcgg ttatgtttat aacgacggca
     1681 cttttcaata tgacgggaga gcgggactca aaaacaataa aagtattgac taccagtttg
     1741 gaaactatgt aaaaggtact tttgccgata cttgcttaaa tattggcaat gcagatgatt
     1801 atacagagtg ggaatataaa actaaaagcg gagtaagggt ttcactggcc ctgggcaata
     1861 gtaaggcatt ggttatcgca gatcttccca attcattcat cacgataaat gtgctttccg
     1921 gggcacaagg aggtttgttc tcagactcag atactattac agccgaagtt ttgcagaatt
     1981 ttgcggattc ctttgatttt tccaagataa aatagttcca ataagcgttg attatttgcg
     2041 ttttaaacgg gtgcagagta atataataag gatatttttc caattaacgg gcaggcatgt
     2101 tttgcttgcc tttttatatt gcagtcctta ttataaacgg attagtggat acaacatact
     2161 attgcaaata aagacaagat gcaataaaat actgtatatg acaactattt tgcagtgtgt
     2221 tattgtatat gcggtggttg agtcgaaaaa agcagcccgg ttgcaagata atccgctatt
     2281 atgtagaata tcaagaggtt aaaaagggga tagtcgaatg caggaaaatg aaaacaagtc
     2341 tcaccgttct caaataacat cggatgacaa taaaaaaact acagagtgta tatcaattgc
     2401 cgtaccttgt tataatgagg aaaaaacatt gcaggcaatg tacaatacgt tgtgtgagat
     2461 agcagcttca atgcgtgagg tggacttcga atttatcttt gttgacgacg gttcgaaaga
     2521 taaaaccctg gaaatgatga gagagatagc ttcaaaagat tcaagagtcg tatatatttc
     2581 tttttccaga aactttggca aagaggcagc catgtacgca ggaatgcaaa aggccaaggg
     2641 agattatgtt gctattatgg atgcagacct gcaggacccg ccggctttgc ttcccgaaat
     2701 gtacagggta attaaagaag aaggctatga ctgtgccgct gcccgccgcg ttaaccgtat
     2761 aggtgaaccg aagatccgtt ctttttttgc acgccggttt tataaaatca tgagaaaact
     2821 cagcaacatg gaagttgtgg acggtgccag ggatttccgt ttgatggagc gtgaaatggt
     2881 gaatgccgtg ctatcggttt gcgaatataa ccgtttttcc aaaggaattt tttcatgggt
     2941 gggttttagg accaaatggc tggagtacaa gaatgttgaa cgcatcggcg gtaatagcaa
     3001 atggtcgttc tggaagctgt ttctttattc tattgacggt attgtggcgt tttccactgc
     3061 acccctggct ttatcctcgc ttttgggcat ttgtttttgc attatcgcgt ttttggcaat
     3121 catttttaca attgtccggc agctggtttg ggggggatcg gcatacggct ggtcgtctct
     3181 ggtgtgtatt atacttcttt tgtcgggaat acagctgttt tgtatcggaa tactgggaca
     3241 atacctctca aagacgtatt tggaagccaa gaaacgccct atatttatag tgagagaaca
     3301 aaaaggagga cacaatgaaa aagaatgata atagaaaaat aataatagcc cagctttgct
     3361 tttggattcc ggcactgatt atgctcgcag catttgcgaa gatgggtatg gcacccttcg
     3421 gtaaaagctc gactatgatt atggacatgt ccgaccagta tgttgaattt ctttgcggct
     3481 tgaaatacgg agatatttac tattcctggt caaaaacatt gggtggaaac tacataggca
     3541 tttttgccta ctatgtttca agtcctctat cggtttttac actgttctgg cccaattcac
     3601 agatgccgat tgccgtgttg tttctgacgg tgattaaaat cggtcttgca ggctttacct
     3661 tcagtttgtt cttaaagtac aggttccgca ggtatgattt gtctacggtg ctgttttcaa
     3721 taatgtacgc acttatggcc tataatgttg tgtattccat gtgcattatg tggctggatg
     3781 cggttatttg gctgccggtt atacttatag gtgttgagaa ggttctcaaa ggaaagagcc
     3841 gtaaactctt gatttttgcg ttgtttgtgt ctttcatatc cacatattat atttcctata
     3901 tgaccggact ttttacagca ttgtatttct tgtaccggtg cgtggagaaa aagctttgga
     3961 gcaagaaact gttgacacgt gtgtggctgt ttgcttccag cgtatttttg gcggcttccc
     4021 tgggagcttt tttcctgctt ccgactctgg agtctttgct cagcggagaa ttggggtcat
     4081 cggggatggt atacagcagc acatttaact ttaaattcgt tcaatttc
//
LOCUS       DAOOJC010000212         4118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16888_length_4118_cov_2.909673, whole genome shotgun sequence.
ACCESSION   DAOOJC010000212 DAOOJC010000000
VERSION     DAOOJC010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16888_length_4118_cov_2.909673"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PLA01_06605"
     CDS             <1..702
                     /locus_tag="PLA01_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD01006.1"
                     /translation="IVVWEVFVRSGDIKEYILPAPSAILNEFKNSGDLLIKHSLVTVS
                     ETVLGFILGTLIAVLLSLLMSNIKAFRSIFYPFMVLSQTVPIIVIAPLITIWFGIGIV
                     SKLIVCVLVVFFPITLNLTEGLDSYDRDLEELLMSMNANKLQIFTKLKLPSALVHFFS
                     GLKIAAAYAVMGAVMAEWTGAESGLGIFLTRSMKSFRTAAMFADIAIISAFSILLFII
                     ISLVEKKSIKWNLKG"
     gene            709..1764
                     /locus_tag="PLA01_06610"
     CDS             709..1764
                     /locus_tag="PLA01_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPD01007.1"
                     /translation="MKRKFAILLCITVILAFAAAGCSNNSNNVPGVSPSGDIDNPGQD
                     KPLANAGLTKINFVLDWVPNTNHTGIYVAKVKGYFEEEGLDVDIIQPGESSADQLVAS
                     NTAQFGISYQEGVTFARDSGVPLVSLAAVIQHNTSGFGWLKDKGIESPKDFEGKKYGG
                     WGSEVEEAMVKQIVKDAGGDPNKVEILTTGTSDFLQASETGQIDFAWIFEGWDLINVK
                     NKGVEINYISLRVLSDVFDYYTPVIVTNDNNIDNNPQLVEKFMRAAQRGYQFAIENPD
                     EAAECLLQLAPELDRELVIKSQQYLAEKYQDHAPYWGMQKKEVWERYMNWLLENGFIK
                     SPIDVEKAFTNDFLKKD"
     gene            1787..2539
                     /locus_tag="PLA01_06615"
     CDS             1787..2539
                     /locus_tag="PLA01_06615"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD01008.1"
                     /translation="MKTIVKIENLKKVYTGKKETLEVLRNINIELLENQFVSIIGPSG
                     CGKSTILRILARLEKESSGNITINGEDIKTSSYRPGYMPQKDLLMPWRTLYKNIVLPL
                     EINGTLKDVEKYDVAGLIKEFGLEGFENYYPSEISGGMKQRAGLLRTFLMGSDIMLLD
                     EPFGALDAITRINMQEWLLKVLVEHKKSVLFITHDIEEAIFLSDRIYVLSDRPACVIK
                     TLDIEFPRPRNREVLSSHKFLEYKDALLKVLL"
     gene            complement(2789..4042)
                     /locus_tag="PLA01_06620"
     CDS             complement(2789..4042)
                     /locus_tag="PLA01_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPD01009.1"
                     /translation="MRKNVLLLVVTLTTIVLSMMFAGAATLYGDLNADGSVDSTDVTI
                     MKRVLLKKMTIEDVVAADLDGDGEVNSTDLTLLKRYLLKKITKFPVENVDPTITPTPT
                     QEITPTPTEINEEIFAFKIRTDSKGEIYQFPIESGEENYNIIVDWGDETTSMITDFST
                     RGHKYEKAGTYTIKVLSFDYMPISFSDDTRLIEVLMPLPDIKATSFKYSFYGCTNLTE
                     IPEALFANNINATSFQYCFYGCSNLTQIPDGLFANNINATSFVFCFYGCSNLTQIPDG
                     LFANNINATDFSFCFSGCNSLSTIPEGLFDNNIIATKFISCFSGCSSLEKIPYGLFDN
                     NINATDFNSCFSGCSSLTEIPYGLFDNNINVTDFSNCFYACSNLTGLAPTLWLRTNVI
                     YYGSCYYGCTELSNYKDIPYDWKFR"
BASE COUNT         1268 a          751 c          822 g         1277 t
ORIGIN      
        1 attgttgtat gggaagtatt tgtaagaagc ggcgatatta aagaatacat acttccggct
       61 ccctctgcga ttttaaatga atttaagaat tccggagacc tgttgataaa gcattcattg
      121 gtaactgttt cggaaaccgt tttggggttt atattgggta cattgattgc tgtgctgctt
      181 tctttgttga tgagtaatat aaaagctttc cgcagcattt tttatccctt tatggttttg
      241 tcccagaccg tacccattat agttattgca cctttgatta ccatatggtt tggaataggt
      301 atagtgtcca aattgattgt ttgcgttctg gtggtgttct tccccattac tctgaaccta
      361 acagaaggat tggactctta tgaccgggat cttgaagaac tattgatgag tatgaatgcc
      421 aacaagcttc agatttttac aaaattgaaa ctgccctcgg cactggttca ctttttttca
      481 gggcttaaaa tagctgcggc ttatgctgtg atgggagctg ttatggcgga atggaccgga
      541 gccgaaagcg gactgggtat ttttttgaca aggtccatga agtccttcag gactgccgca
      601 atgtttgcag acattgcaat tatttcggct ttcagcattt tattatttat aattataagt
      661 cttgttgaaa agaaatctat taagtggaat ttgaaaggat gagcgaaatt gaaaagaaaa
      721 tttgcaatat tattatgtat aacggttata cttgcttttg ctgccgcagg atgcagcaac
      781 aattctaaca acgttccggg agtcagtcct tccggagata tagacaatcc cggacaggac
      841 aagcctttgg caaatgcggg gcttacgaaa atcaactttg ttcttgactg ggtacccaac
      901 acaaatcaca cgggaatcta tgtggcaaag gttaaaggct attttgaaga ggaagggctt
      961 gatgtcgata taatccagcc cggtgagtct tcagcggatc agcttgtggc ctccaacacc
     1021 gcacagtttg gaatcagtta tcaggaagga gtaacatttg cccgggattc gggtgtaccc
     1081 ttggtatctc tggcagcagt tatacaacac aatacttctg gctttggctg gcttaaagat
     1141 aaaggaattg agtctcctaa agattttgag ggcaaaaagt atgggggctg gggctcagag
     1201 gttgaagaag ccatggtaaa gcagattgtc aaggatgcag gcggagaccc caataaggta
     1261 gagattctga caaccggtac atccgacttt cttcaggcaa gtgaaaccgg tcaaattgat
     1321 tttgcctgga ttttcgaagg ctgggacctg ataaatgtaa aaaataaggg agttgaaata
     1381 aattacatca gccttagagt gctttccgat gtctttgact actacacccc tgtaattgtg
     1441 acaaatgata ataatataga taacaaccct cagcttgttg agaaatttat gagggcagct
     1501 caaaggggtt atcaattcgc cattgaaaac cctgatgaag cagcggaatg tcttttgcag
     1561 ttggccccag agcttgacag agagcttgtg atcaagagcc agcagtattt ggctgaaaaa
     1621 tatcaggatc atgcaccgta ttggggtatg cagaaaaaag aagtctggga aaggtatatg
     1681 aactggcttt tggaaaacgg attcataaaa tcgccgattg atgttgaaaa ggctttcacc
     1741 aatgactttc taaaaaaaga ttaaaaagcc tttatgggag taaataatga aaaccatagt
     1801 aaagattgaa aacctaaaaa aagtatatac aggtaaaaaa gaaactcttg aggtcttacg
     1861 caatataaat attgaacttc tggaaaacca gtttgtatcc ataattggac ccagcggctg
     1921 cggtaagagc acaatactta ggattctggc acggctggaa aaggaatctt caggaaacat
     1981 aacgataaac ggagaggata ttaaaacttc ctcctaccgg ccgggttata tgccccaaaa
     2041 agacctttta atgccctggc gtacactata taaaaacatt gttcttcccc ttgaaataaa
     2101 cggtacactg aaagatgttg aaaaatatga tgttgctggc cttatcaaag aatttgggct
     2161 tgaaggcttt gaaaactact atccgagtga aatatccggc ggaatgaagc aaagagccgg
     2221 acttcttcga acctttttaa tgggcagcga tataatgctt ttggatgagc cctttggtgc
     2281 ccttgatgca ataacccgga taaatatgca ggaatggttg ttgaaagtat tggttgagca
     2341 caaaaaatcc gtattgttta ttacccacga cattgaagag gcaatttttc tgtcggacag
     2401 gatatacgtt ctatccgaca ggcctgcctg tgtcatcaaa accttggaca tagaattccc
     2461 aaggccgaga aaccgtgagg tactttcttc tcataaattt ctcgaatata aggatgcctt
     2521 gctcaaggtg ttattgtgaa aatcggatag ttattttttt gaattaccgt gtttataaaa
     2581 tataattttt aaaattattt attgtattta tccttgtttt tttcgataca gaaatacaga
     2641 atcatttctt tataaaatta ttaaaggata cacaaacaag catcagctct ttgtctatcc
     2701 ttttccttat tagtcggttc ctgatgacgt attgtctaaa atcattgaaa aattagctca
     2761 accctagtga catccttaat cttacgcctt accggaattt ccaatcatat ggaatatctt
     2821 tatagttaga tagttcagta cagccgtaat aacaagaacc gtaatatatt acatttgtcc
     2881 gcagccataa tgtcggggct aagcctgtta ggttgctgca tgcataaaaa caattgctaa
     2941 aatcagttac attgatatta ttatcaaata acccatacgg aatttctgtt aagctgctgc
     3001 atccagaaaa acaactgtta aaatctgttg cattaatatt attatcaaat aacccatacg
     3061 gaattttttc taagctgctg catccagaaa aacaacttat aaactttgtt gcaataatat
     3121 tattatcaaa taatccttcc ggaattgtac tcaagctgtt gcatccagaa aaacaaaaac
     3181 taaaatctgt tgcattgata ttattagcaa ataacccatc aggaatttgt gttaggttgc
     3241 tgcaaccata aaaacaaaaa acaaaacttg ttgcgtttat attattggca aataacccat
     3301 ctggaatttg tgttaggttg ctgcaaccat aaaaacaata ctgaaaactt gttgcattga
     3361 tattattagc aaataacgct tccggaattt ctgttaggtt tgtgcatcca taaaaactat
     3421 atttaaaact tgttgctttg atgtctggta aaggcataag tacttcaatc aatctggtat
     3481 catctgaaaa actaatcggc atataatcaa aggaaagtac ctttattgta tatgtacctg
     3541 ctttttcata cttatgacct cttgtagaga aatcagttat catgcttgtt gtttcgtcgc
     3601 cccagtcaac aataatatta taattttctt ctccagactc aatgggaaat tggtatatct
     3661 ctcctttgct atccgttctt atcttgaatg caaaaatttc ttcattgatt tcagtaggag
     3721 ttggtgtaat ttcctgcgtg ggagttggtg ttattgtggg atccacgttt tcaaccggga
     3781 atttggttat tttcttaagc aaataccttt ttaacagcgt taaatctgtc gaattcactt
     3841 caccgtcacc atctaagtca gctgcaacaa catcttcgat agtcattttc ttaagcagta
     3901 ccctcttcat tatcgttaca tcggtggagt caacagatcc gtctgcattt aagtcaccat
     3961 acagtgtagc ggcaccggca aacatcatgc taagaacaat tgttgtaagg gttactacta
     4021 ataataatac attctttctc atataagcat ctcccccatc tatatttatt aatgctctcg
     4081 aaaactcgta ttgctttcga ggtctctatt tcaggagc
//
LOCUS       DAOOJC010000213         4102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16961_length_4102_cov_3.682728, whole genome shotgun sequence.
ACCESSION   DAOOJC010000213 DAOOJC010000000
VERSION     DAOOJC010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16961_length_4102_cov_3.682728"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLA01_06625"
     CDS             <1..217
                     /locus_tag="PLA01_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01010.1"
                     /translation="GGSDILRGVEVQEIDINEEDYTPYISYGNFTWFNPYEVLKGRKF
                     SYENDGWNIIITIDDKHNIVYVDIYSS"
     gene            341..1279
                     /locus_tag="PLA01_06630"
     CDS             341..1279
                     /locus_tag="PLA01_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPD01011.1"
                     /translation="MNWFKRTALKALSFGTRNLKRYYKVQRKLINVIKIFIKYRYKVV
                     DRKITVGDRKIPVRFFYPKRNPTSGVMVFFHGGGWVTGNVDSYTRVCSNMANQTRNTI
                     ISVDYRLAPEYPFPAGLEDCYNVTREIYRNTDRLNCKAEDITLIGDSAGGNLAAAVSL
                     MARDRGEFLPKRQILIYPSTYNDHSENSPFPSIKENGTGFLLTTKKIKDYMDMYAPNK
                     EDRLNPYLAPLLAQDLSSQPDTLIITAEYDPLRDEGEAYGMRLKEFGNNVKMYRMKDA
                     VHGFFSLPWKSEYVVRSYEIINWFLSSYNGQSEELI"
     gene            1276..2553
                     /locus_tag="PLA01_06635"
     CDS             1276..2553
                     /locus_tag="PLA01_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01012.1"
                     /translation="MNNPKKVEWSKLDNASKYFPATCDERDPKVFRIACELFEDVEPE
                     ILQQALDITIEQFPSYKSVLRRGIFWYYYESSDIRPTVKREEKPVCAPIYRKYEKNLL
                     FRVFYNNKRINIEVFHALSDGTGALWFMMTLLYHYLLIKHKDAFLDKKPELNYNASIS
                     QKKDDSFSRYYQGRHYKKQAKVKKEKKNARKVYLIRGSRLEENRIKVIEGTMSAKAVL
                     DEAHKFNTTLTVFLSALLLLSIYKDMPVRKRGNTLVLAVPINLRQFFESETSSNFFSI
                     MNIPYTFDEGNTELKDVIGKLNESFRKELTEERLYQHLHRLMSFERNPFARIAPLPLK
                     NLSIRGVNAVADRGVTTSISNLGKIHVPPEFDSYIRQFSVVPNVRRPQMAICTYGDRL
                     VINFASPFRETEIQRFFFESLANMGIKIEIASN"
     gene            2565..3233
                     /locus_tag="PLA01_06640"
     CDS             2565..3233
                     /locus_tag="PLA01_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HPD01013.1"
                     /translation="MIFCNHCKVYIRDGKTICPLCGNILSDNDNDVKGEEIYPDIPLT
                     YNRHMVIRILVFISIVAIVVSYTVYKMVPVDVNWPGLVLLGLVSIWLSLTNILRKRHN
                     ITKSIMWQVIIVSVLTVFWDWRIGWRGWSLDYAIPILCIAAMLVMYITAKVMKLSARE
                     YISYFLLGGLFGIVPILFILFDLVNMSIPSVISVAASIVFLSAIIIFHGDSIKAELQK
                     RMHI"
     gene            complement(3249..3902)
                     /locus_tag="PLA01_06645"
     CDS             complement(3249..3902)
                     /locus_tag="PLA01_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPD01014.1"
                     /translation="MEMRKTYKVVIADDQELIRDCIKKYIEEDSEIEVVDCVENGKLA
                     FEVCKKYKVDVVLMDMVMPDYDGITATKKIIELNKNIKVLILTSFPDSSQLVDAVKIG
                     VSGYITKDSSAKDMIMSIKNTANGNLVFEKSVFDGILKNIMEEKKSEPDIFQDLNENE
                     FAVLSILAIEGADNKDIAAKTYLSESTVRSIISKLMNKYGLTKRTQLAVFALKNGLS"
BASE COUNT         1290 a          647 c          832 g         1333 t
ORIGIN      
        1 tggcggttct gacatattaa gaggggtgga ggtacaagaa atagacatta atgaagagga
       61 ttatacacct tatataagtt atggaaattt tacttggttt aatccgtatg aagttctaaa
      121 aggaagaaaa ttttcgtatg agaatgacgg ctggaatata ataataacaa ttgatgataa
      181 gcataatata gtttatgttg atatttatag cagttgagat ttgcgaaaag gatcagtacg
      241 cacaaagaat accaaaaaat gtctaatatc attgcaggta tgttgtggat atagtaaaat
      301 ataattattg gattatgcgg tcttagaaag gacacatggg atgaactggt ttaaaaggac
      361 agcattaaaa gctttatctt ttggcaccag aaacctgaaa aggtattaca aagttcaacg
      421 caagctgata aatgtaataa agatttttat taaatacaga tacaaggttg tagaccgtaa
      481 aataactgta ggtgacagaa aaatccctgt gcgttttttc tatcccaaga ggaatccaac
      541 ctccggtgtt atggttttct tccatggagg cgggtgggtg accggaaatg ttgattctta
      601 tacccgtgtt tgttccaaca tggcaaatca aacaaggaat actattattt ccgttgatta
      661 tcgtttggct ccggaatatc cctttcctgc aggacttgag gactgttaca atgtaacgag
      721 ggagatatat agaaataccg ataggttgaa ttgtaaagcg gaagatatca cattgatagg
      781 tgacagtgcc gggggaaatc tggcagctgc agtttctttg atggcaaggg accgaggcga
      841 atttttaccg aagagacaga ttttaattta tccgtctaca tataatgacc actcggaaaa
      901 ttccccattt ccttcaataa aggaaaatgg gacaggtttt ttgctgacca ctaaaaaaat
      961 aaaggattat atggatatgt atgctccgaa taaagaagac aggctaaacc cttatcttgc
     1021 accccttttg gcacaggacc tgtcgtcaca gccggatacc ttgattatta ctgcagaata
     1081 tgatcccctg cgggatgagg gggaggcata tggaatgagg ttgaaggaat tcggaaacaa
     1141 tgtgaagatg tatcgaatga aagatgctgt ccatggcttc ttttcgttgc catggaaatc
     1201 ggaatatgtt gttagaagtt atgagattat aaattggttt ttaagcagct acaacggtca
     1261 aagtgaggaa ttgatatgaa taatcctaaa aaagtcgaat ggagtaagct ggataatgcc
     1321 tccaaatatt ttccggcaac atgtgatgag agggacccaa aggttttccg tattgcgtgt
     1381 gagctgtttg aagacgttga accggaaatt ttacaacaag ctttggatat taccattgaa
     1441 caatttccat catataagtc cgttttaaga agaggaatat tctggtatta ttatgaaagc
     1501 agtgatattc gcccgacggt taaaagggag gaaaaaccgg tttgtgctcc gatctataga
     1561 aaatacgaga aaaatttatt gtttagggtt ttttataaca acaaaaggat caatattgaa
     1621 gtatttcacg cactttccga cggaacaggt gccctttggt ttatgatgac acttttatac
     1681 cattatttgt taatcaaaca caaggatgct tttttagata agaaacccga attgaactat
     1741 aatgcatcga tcagtcagaa aaaggatgac agcttcagca ggtactatca agggagacat
     1801 tataaaaagc aggcaaaagt taaaaaagag aaaaagaatg ctagaaaagt ataccttatt
     1861 cgggggtcca gacttgaaga aaatagaatc aaagtaattg aaggaacaat gtctgccaaa
     1921 gccgtccttg atgaagccca taaatttaac actacattaa ccgttttttt atcggcatta
     1981 ttgcttcttt caatatataa ggacatgcct gtacgtaaga gagggaacac attggtatta
     2041 gcggtgccta taaatcttcg gcagtttttt gaatctgaaa catcaagcaa ttttttcagc
     2101 attatgaata ttccttatac ttttgacgaa ggaaatactg agcttaagga tgtaataggt
     2161 aagcttaacg agagctttcg taaagaatta acggaagaaa gattatatca gcatcttcat
     2221 cggttaatgt cttttgagag gaatcccttt gcaaggattg ctcctttgcc tttgaaaaac
     2281 ctttctattc ggggcgtaaa cgcagtagcg gacagaggtg taacaacatc tatatccaat
     2341 ctgggcaaaa ttcatgtgcc tcccgagttt gacagctata tccggcagtt cagtgttgtt
     2401 cctaatgtta gaaggcctca gatggcaata tgtacttatg gagaccggtt ggtaataaat
     2461 tttgcgtcac catttcggga aaccgaaata caaaggtttt tctttgaatc ccttgcaaat
     2521 atggggatta aaattgagat tgcttcgaat taggagttga tagaatgata ttttgcaacc
     2581 attgcaaggt ttatatacgg gacggcaaaa caatatgccc tctgtgcgga aatattcttt
     2641 cggataatga caacgatgta aaaggggagg agatttatcc cgacataccc cttacttata
     2701 ataggcatat ggttattcgt attttggtgt ttatatctat tgttgccatt gtggtgagct
     2761 atactgttta taaaatggta cctgttgatg tcaattggcc gggactggtc ttgttgggct
     2821 tggtcagtat atggcttagt ctcactaata ttttgcgtaa gagacataat attacaaagt
     2881 caattatgtg gcaggtaatc attgtctcag tgcttactgt tttttgggat tggagaatag
     2941 ggtggagggg ctggtcgttg gattatgcca ttccgatttt gtgcatagca gcgatgcttg
     3001 ttatgtatat aacggcaaaa gtaatgaaac tcagtgcgag agaatatatt tcatactttt
     3061 tattgggagg gttattcggt attgttccta ttctgtttat tttgtttgat cttgtaaata
     3121 tgagcattcc atccgttatc tcagtggctg ccagtatagt atttttatct gccataatca
     3181 tatttcacgg ggacagtatt aaggccgagc tgcaaaaaag gatgcatata tgatataaag
     3241 catatagatt aactaagccc gttctttaaa gcaaatacag caagctgagt tcgtttagtt
     3301 aatccatatt tattcattaa ttttgatatt atactcctta cagtactctc gctaagataa
     3361 gtctttgcgg caatatcttt gttatcggca ccttctattg caagtatgct cagcacagca
     3421 aattcatttt catttaggtc ttggaaaata tctggttccg attttttttc ttccattata
     3481 tttttaagga ttccatcaaa aacgcttttt tcaaaaacta aatttccatt ggctgtattt
     3541 tttatactca ttatcatatc tttagcagat gaatccttag ttatgtatcc gcttacacca
     3601 atcttaacag catcaaccag ttgagaactg tcaggaaagg aggtaagtat aagtactttt
     3661 atgtttttgt tgagctctat aatctttttt gtagcggtga taccgtcata atctggcatt
     3721 accatatcca taagaactac atctacttta tattttttgc atacttcgaa agcaagtttg
     3781 ccgttttcaa cacagtctac tacctctatt tccgagtctt cttctatgta tttttttata
     3841 cagtctctta ttagttcttg atcatctgct attactacct tgtaagtttt tcgcatttcc
     3901 atagagtgca cccccaaaat taagtctgat atagatagtt taatatgtaa aaagttatca
     3961 atataaataa tcggttatct attaatgtta ttttgttaat attatacaac atttttgata
     4021 tttagcatat tgaatgagtg acaaatatcg atgaattatg aaaaagtttg aacaaatttg
     4081 tatacttatt ttatactttt ta
//
LOCUS       DAOOJC010000214         4101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_16970_length_4101_cov_2.987642, whole genome shotgun sequence.
ACCESSION   DAOOJC010000214 DAOOJC010000000
VERSION     DAOOJC010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16970_length_4101_cov_2.987642"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="PLA01_06650"
     CDS             complement(<1..981)
                     /locus_tag="PLA01_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HPD01015.1"
                     /translation="MKLNRLFFIIIVLQSLLFSGCWSSHEVNTLGITVCIGIDKTENG
                     YLVTKQILNPSTIASDREMTESPIVVYSVEVSSMAEVNGRLMMKLSRKINNTHLRLVV
                     FGEDVAKEGIENILDYFARNHEFRTDYYFAVARNATAKEILGILTPIEMVPGVDMYNS
                     LKMSEDEWASIKSIRVIELVNSIMAEGKNPVLTGIKIDRKNKINANSTEILKLTDEYN
                     LLELSNMCAFNKDKLVGWLDEDESKGYNYITGNVKRTAENAYNDQVKISSMIMHVKSN
                     TRVNLSENEPFIEVKIKIEQNIRSIEGNFDISSKENKQVLNEMFESKVKSL"
     gene            complement(990..2552)
                     /locus_tag="PLA01_06655"
     CDS             complement(990..2552)
                     /locus_tag="PLA01_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HPD01016.1"
                     /translation="MKFFSNTTTKGKRTKQANSDISKDINDNIDRLKNDFADDSDLVI
                     HRFKLGNVSGLVCANIYIKSLVDRNTINGLSVALNKLKCECINGESDINVDELMNYFS
                     GISEGKEGLDYETLCLDLLSGSTVFLIDGCERYLAVDASSNEGRSVDEASSQTVIKGP
                     KDGFTEKINSNILLIRKRIKSKDLKVEYLNVGSITKTTVGLMYLHKIARDDIVQELRT
                     RLNKIEIDGVLESGYIEELIKDDRYSIFPTFLSSEKPDSVTAALLEGKVAILVDGTPY
                     VLTAPALMIEFLQSSEDYYHHYIISSMMRLLRMLAFFLTLLVPAVYIAVTLFHQEIIP
                     TPLLISLAAQREGVPFPVLLEALIMEITFEILREAGIRMPRAIGSAVSIVGALVLGQA
                     AVEAGVISAFMVIVVSITAISSFAIPNYEMSNAIRLVRFFLMLMAGMFGLYGILIGLI
                     VLVLHLCKIKSVTVPYLTPVAPKVKGTITDTLFRFPLWKMKYRPIGISGTDEARSGES
                     NPVSSGQKKSRN"
     gene            complement(2614..>4101)
                     /locus_tag="PLA01_06660"
     CDS             complement(2614..>4101)
                     /locus_tag="PLA01_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPD01017.1"
                     /translation="EISHGGKVEYLNNCYRSTKNIITVVNSIFSRLLIPYEELRCPKE
                     DSEAGRVMLITWEKDLPKKNKPKTRWEMAKDLLLSEDMAQELKDVLEAEYESTIASGK
                     KDYQGDVISGVIRKLTAEGLGYRDMAILLRSRTSLPEIENSLTENGIPYCVIGGIGFW
                     DRQEVGDILSLYKLVFDPDDRLALFTVLRSPIFGFSDDLLLLLSLFIRQQEIRGIREL
                     MREFATAVSKEDRWLVERAVAVFDSLLPMEGLLNSLELMNKIIEATAYDEILTALPQG
                     EKKLRNMEKLVRIVEEFDKKGLYSARELLSYIETLKEHSGLSSEAFLDNEDSDAVKIL
                     TIHASKGLEFEAVLLPDMDRPLSTQTKRDKPLFFPDEKKGVVAMGLDGNCEFDENANP
                     EYGKLYDQRLLKESEESRRLFYVAATRAKKYLALIGERQEVGEGEEIDEQNSFMKQLV
                     WAMNKAGTVEEIDLIDAQSLIFDSNEQNEYPPLFIAEINKLLEYI"
BASE COUNT         1121 a         1008 c          666 g         1306 t
ORIGIN      
        1 caatgattta accttgctct caaacatttc atttagtacc tgcttgtttt cctttgaaga
       61 aatatcaaaa tttccttcaa tgcttctgat attttgctcg attttgattt tcacctcaat
      121 aaaaggctca ttttcagata aattcactct agtattggat ttgacatgca taatcatgga
      181 tgaaattttc acctggtcat tgtatgcatt ttctgctgtc ctcttaacat ttccggtgat
      241 ataattatat cccttgctct catcctcatc caaccagcct acaagtttat ctttattaaa
      301 agcacacata ttagataatt ccagtaaatt atattcatca gtcagtttca agatttctgt
      361 ggaattggca ttaattttat tttttctgtc aatttttata ccagtaagca cgggattttt
      421 cccctctgcc ataatggagt taaccagttc tatgactctt atagatttta tggacgccca
      481 ttcatcttca gacatcttaa gtgaattata catatcgact ccgggaacca tttctatagg
      541 agtcaatatg cctaaaattt ctttggcggt tgcatttctg gccaccgcaa aataataatc
      601 ggtacggaat tcatgattgc gtgcaaagta atccagtata ttctctatac cctcttttgc
      661 aacatcctcg ccaaatacca ccaacctcaa atgtgtgttg tttatcttcc ttgataattt
      721 catcataagc cttccgttta cctctgccat gctgcttacc tctactgaat atacaacaat
      781 cggtgactcc gtcatctctc tatcggaagc tatagtactg ggatttaaaa tctgctttgt
      841 aaccaaatat ccgttttctg ttttgtcaat accgatacat acggtaattc caagggtatt
      901 tacctcgtgg ctgctccagc atcctgaaaa taatagggat tgtaaaacta ttataataaa
      961 aaaaagccta ttaagtttca tatgctacct caattcctgc tttttttttg acctgaactc
     1021 acagggttgc tttcgccgct tcttgcctca tcagttccac tgataccgat gggtctgtat
     1081 ttcattttcc ataatgggaa cctgaaaagg gtatccgtaa ttgtgccctt taccttcggt
     1141 gccaccggtg tcagatacgg aactgttacg gattttattt tacaaagatg aagcaccaaa
     1201 acaataagcc ctatcaatat tccatacagt ccgaacatac cggccatcag cattaaaaaa
     1261 aaccttacca gccttatagc attggacatt tcataattgg gtatggcaaa gctcgatatg
     1321 gcagtaatgg aaacaacaat aaccataaac gcagatataa caccggcttc caccgccgcc
     1381 tgccccagta ccaaagctcc tactatggaa accgcagacc caatggccct gggcattctt
     1441 attccggctt cccttaatat ttcaaaggtt atttccatga tcaaggcctc caataaaacc
     1501 ggaaaaggaa ctccttcccg ctgtgccgct aagctgatga gcaatggagt gggaataatt
     1561 tcctgatgga ataaggttac tgctatatag actgccggta ccaaaagcgt aagaaaaaag
     1621 gcaagcatcc ttaataaacg catcatggaa gatataatgt agtgatggta ataatcctca
     1681 ctggattgga gaaattctat cattagggca ggggcagtca atacatacgg agtaccgtcc
     1741 accagaattg ccacctttcc ctcaagtaaa gccgctgtaa ccgaatcagg cttctcggag
     1801 cttaaaaaag tcgggaaaat agagtaacgg tcatctttta tcaattcctc aatatatccg
     1861 ctttccagta cgccgtcaat ctctatttta ttgagccttg tccttaattc ctgaactata
     1921 tcatccctgg ctattttgtg aaggtacatc aaaccaactg ttgttttggt tatactgccc
     1981 acattcaaat attccacttt taaatctttg gattttattc tttttctaat aagcagaatg
     2041 ttactgttta tcttttctgt aaaaccgtct ttagggcctt tgattacagt ttgagaagaa
     2101 gcctcatcca ctgacctgcc ttcattggaa cttgcatcta ccgcaagata cctttcacaa
     2161 ccgtcaatta aaaacacggt actcccggac aacaaatcta aacaaagagt ctcgtaatcc
     2221 aaaccttctt tgccctcgct tattccggaa aaatagttca ttaactcatc aacatttatg
     2281 tcggattcac cattgatgca ttcgcatttc agtttattca gcgctactga caatccgttt
     2341 atagtatttc tgtccaccaa gctttttata tatatattgg cacaaaccaa gccgctgacg
     2401 ttaccaagct taaagcgatg tattacaaga tcgctgtcat cggcaaaatc attctttaac
     2461 ctgtcaatat tgtcatttat atctttactt atatcagaat tggcttgctt tgttctttta
     2521 ccctttgtag tcgtattact aaaaaacttc atttaattac actatctccc cgctgctacc
     2581 ctatattttc atatagtatt ttttccaatg ccgtcaaata tattctaaga gcttattaat
     2641 ttctgcaata aacagcggag gatactcatt ttgttcattt gagtcaaaga taaggctttg
     2701 ggcatcaatt aaatctatct cttccactgt accagccttg ttcatcgccc aaaccaactg
     2761 tttcataaaa ctgttctgtt cgtcgatttc ctctccttct ccaacctcct gtctctctcc
     2821 aatcaaagcc aaatattttt tagcccgggt ggcagccaca taaaacaacc tcctggattc
     2881 ctccgattcc ttaagaagcc tttgatcata cagcttccca tactcaggat tcgcattttc
     2941 gtcaaattcg cagtttccat caagccccat ggctacaacg cccttttttt catccgggaa
     3001 gaaaagaggc ttgtcccttt ttgtttgggt gctaagcggt ctgtccatgt ctggaagcaa
     3061 taccgcctca aactcaaggc ccttggaagc atggattgtc aatattttta ccgcatcgct
     3121 gtcttcattg tcaaggaaag cctcactgct aagccccgag tgctctttta aagtctcaat
     3181 ataagaaagc agttcccttg cactgtacaa tcctttttta tcaaactctt caacaatcct
     3241 tacgagcttt tccatattcc tgagcttctt ttccccctgg ggcaatgctg taagtatctc
     3301 gtcataggca gttgcctcaa tgattttatt catcagttcc aggctgttta acagtccttc
     3361 catgggaaga agactgtcga acacagctac ggctctctcc accagccacc ggtcttcttt
     3421 tgaaacggcc gttgcaaatt ctctcatcaa ctctctaatg cctcttattt cctgctgcct
     3481 tataaataga gaaagtaaaa gcaaaaggtc atcggagaac ccgaatatcg gcgaacgcag
     3541 cactgtaaac aatgcaagcc tgtcatcggg atcaaacacc agtttataca gcgatagaat
     3601 gtcccctact tcctgccggt cccaaaaacc gatcccccct atcacacaat aaggaatacc
     3661 gttttctgtc agggaattct ctatctccgg gagggatgtt ctgctcctca aaaggattgc
     3721 catatccctg taccctaatc cttcggcggt cagctttcgg atgacaccgg aaatgacatc
     3781 cccctggtaa tccttcttac ccgaagctat ggtgctctcg tactcagcct ccaatacatc
     3841 ctttaactct tgtgccatgt cttccgacag cagcagatcc ttagccattt cccaccttgt
     3901 tttgggcttg ttctttttag gcaaatcctt ttcccaggta attagcataa cccttccggc
     3961 ctccgaatcc tccttggggc accttagctc ttcatagggg attaaaagcc ggctgaatat
     4021 gctgtttacc acagtaataa tatttttagt gcttctatag cagttgttca aatactccac
     4081 ctttccccca tgggaaattt c
//
LOCUS       DAOOJC010000215         4082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_17068_length_4082_cov_3.407996, whole genome shotgun sequence.
ACCESSION   DAOOJC010000215 DAOOJC010000000
VERSION     DAOOJC010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17068_length_4082_cov_3.407996"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /gene="murF"
                     /locus_tag="PLA01_06665"
     CDS             <1..776
                     /gene="murF"
                     /locus_tag="PLA01_06665"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPD01018.1"
                     /translation="NILKAKMEILEGLNEKGLVILNGDDSLLFGLKGLLNYRTVFYGL
                     EEGMDYQGYNVVSLGEHGSTFDINIGNREYSVKIPVPGLHNIYNALAGIAAGIELGIP
                     PEEIIKGIEEFVPGKMRLNIINHNGLKIINDVYNASPQSMEAAINVLKDISAGNRTVA
                     VLGDMLELGEISEKAHFDVGKYAVSNGINYVAAVGEYRDNTAGGAIDAGAKKDNVFVF
                     NNNNEAASFLKYFLKPGDVLLVKGSRGMKMEEIVNMLTG"
     gene            861..1847
                     /gene="mraY"
                     /locus_tag="PLA01_06670"
     CDS             861..1847
                     /gene="mraY"
                     /locus_tag="PLA01_06670"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPD01019.1"
                     /translation="MELPFDISTHVLIFILSFACVLVLGPVLIPLLARLKFGQTVRDD
                     GPKSHYKKMGTPTMGGVIFLIPIAVISAFYARYDTRILPLLFVTLGFGLIGFIDDFIK
                     VVKKRKDGLYWNQKMFGLLVIAVTFAVYVSRTQSSDIIIPFLGMENTLSLGWLFIPFI
                     IVVLIGSTNAVNITDGLDGLAAGVTLIVTVFFTIVSMTRGEWDFVKIFCSIVSGGCLG
                     FLAFNSHPAKVFMGDTGSLALGGAVGAIAIMMKMPLILIIAGGIYVIEALSVMIQVLW
                     FKATGSRVFKMAPIHHHFELSGWKEVKVVAVFWTISVLFCVIGFLAIRLRFY"
     gene            1967..3082
                     /gene="ftsW"
                     /locus_tag="PLA01_06675"
     CDS             1967..3082
                     /gene="ftsW"
                     /locus_tag="PLA01_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HPD01020.1"
                     /translation="MKSKSTKPFDFLIFMTVMILLALGSVMVFSSGAPSAYNYMGGDT
                     YHFLKKQLVFVPVGIFAMFVMMNFDYRKLGKLSPIIMLVNLALLSVVWIDGIGATRNN
                     ATRWFSFQGFDFQPSELSKLAIILFFSYSLSKRKDTLKYFFKGLVPYLIIIAIHAFLL
                     LLQPHMSAMIIIGMVSCIILFCAGARIKHFMIMGIPAVAAGVCLVIFSDYRRDRIIAF
                     LDPWKYPSNEGYQAIQSLYAIGSGGLFGRGLGNSLQKFLYIPEPYNDFILAILAEELG
                     FIGVATVLILFLIFIWRGVKVAMNAPDAFGTLVATGITSLIAVQVVINVAVVTSSMPV
                     TGMPLPFFSYGGSSLVFLLTNVGILLNISKHAKYERI"
     gene            3095..>4082
                     /gene="murG"
                     /locus_tag="PLA01_06680"
     CDS             3095..>4082
                     /gene="murG"
                     /locus_tag="PLA01_06680"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPD01021.1"
                     /translation="MKVLISGGGTAGHINPGLAIAKYIMKMEPDSKILFVGTQKGLES
                     KLVPRENFDIEMIKVRGFQRKLSFDTLVAVKELFQGLFEARKVIRDFKPDVVIGTGGY
                     VCGPVVFNASRMKIPTLVHEQNAFPGVTNKILSKFVDRVAISFKESTKYFKGRKNVFF
                     TGNPIRSEMLEVSREAARNKLGIPKSESLVVIFAGSRGAENVNSTVSEMIKKYGTDFG
                     FHLIYATGEAQYSGIKERIGDIKSKNVQVLPYIYDMANTMAAADLVVCRAGAITISEL
                     TALGISSVMIPSPYVTANHQEYNAKALEQQGAAVVILEKNLSCDILYNQINTL"
BASE COUNT         1170 a          623 c         1024 g         1265 t
ORIGIN      
        1 ataatatatt aaaagcaaaa atggagattt tagagggctt aaatgaaaaa ggtctggtta
       61 tacttaatgg tgatgacagt ttgttatttg gcttaaaggg cctgttaaat tatagaactg
      121 ttttttacgg actggaagaa ggaatggatt atcagggata taatgtagtg tctttgggag
      181 aacatggttc aacctttgat attaatatcg gaaacaggga gtacagcgtt aaaattcctg
      241 tgccgggact acataatata tacaatgcct tggcgggaat tgcggcaggg attgagctgg
      301 gcataccgcc ggaggagata attaaaggca ttgaggagtt tgtgccgggt aaaatgaggc
      361 tcaacataat taaccataac ggcttgaaaa taataaatga tgtctacaat gcaagtcctc
      421 agtccatgga ggcggctata aacgtattga aggacatatc ggcaggcaac agaaccgttg
      481 ctgttttggg cgatatgtta gagctcgggg agatatcaga aaaagcacat tttgacgtcg
      541 gaaaatatgc cgtatccaac ggaataaact atgttgcagc agtgggagaa tatcgggaca
      601 atactgccgg cggtgctatt gatgcgggag caaaaaagga taatgttttt gtgtttaata
      661 ataataatga agcagcaagt tttcttaaat actttttaaa gccgggtgac gtgctgcttg
      721 taaagggctc aagaggtatg aaaatggagg agatagttaa catgttgaca ggctgattat
      781 taaggtgtaa atttaattaa tgggcttgga aatagaaaaa gaactatgta tttatttagc
      841 atttatgggg gagattaaac ttggaattgc cgtttgatat ttcaacacat gttttaatat
      901 ttatattaag ttttgcttgc gtgctggttt taggacctgt acttattccc cttctggcac
      961 ggcttaagtt tggccagacg gtaagagacg acggtccgaa atcacactat aagaagatgg
     1021 ggacgcctac tatgggagga gtgattttcc ttatcccaat agcagttatt tcggcatttt
     1081 atgcaagata tgatactagg attctgcccc ttctttttgt aactctcgga tttgggctga
     1141 taggatttat agatgatttt attaaagtag tcaagaagag aaaagacgga ttgtactgga
     1201 atcaaaagat gttcggactt cttgtcattg cggttacatt tgcagtctat gtatcgcgga
     1261 ctcagtcttc ggacataata attccgttct tagggatgga gaataccctt tctctggggt
     1321 ggttgtttat accctttata atagttgtat taatcggttc aacaaatgcg gtaaatataa
     1381 ctgacggtct tgacgggctg gcggccggtg tgacattgat tgtaacggtc ttttttacca
     1441 tagtttccat gaccagagga gagtgggact ttgtaaaaat attctgttcc atagtttcgg
     1501 gagggtgtct tggtttctta gcttttaact cccatcccgc aaaggtgttt atgggtgata
     1561 cgggatcgct ggcattggga ggggctgtcg gtgccattgc aataatgatg aaaatgcctt
     1621 taatacttat aattgctggc ggtatttatg taatagaagc tttatcggtt atgatacagg
     1681 tattgtggtt taaggcaaca ggaagcaggg tgtttaagat ggcacctatt caccatcatt
     1741 ttgagctttc cggatggaag gaagtcaagg tggttgcagt cttttggaca atatctgtgc
     1801 tcttttgtgt aattggtttc cttgcgataa ggctaaggtt ttattaaaac tgcaaaacat
     1861 taaacactaa ttttaaatgt gatggaataa acagctaagc aggataatat tgatgtaaag
     1921 tattgaattt attacttcta aggcgaaagc tttgaggggg aaaaggatga aatcaaaatc
     1981 aacaaaaccg tttgattttt taatatttat gaccgttatg atacttcttg ctttgggaag
     2041 tgtaatggtg ttcagttcag gtgcacccag tgcgtacaac tacatgggtg gggatacgta
     2101 tcatttttta aagaagcagt tggtttttgt tccagtgggg atttttgcca tgttcgttat
     2161 gatgaatttc gattacagaa agcttggcaa attgtcacct attatcatgc tggtcaattt
     2221 ggctttgctt tcagtagtat ggattgacgg aataggtgct acccgtaaca atgcaaccag
     2281 gtggtttagt tttcaaggat ttgattttca gccgtcggag ttatcaaagc tggcaataat
     2341 attattcttt tcctacagcc tttcgaagag aaaggatact ctaaagtatt ttttcaaagg
     2401 tctggttcca tatctgatta taatagcgat tcatgcgttt ctattacttt tgcagcctca
     2461 tatgagtgca atgattataa taggtatggt atcttgcata attttgtttt gtgccggggc
     2521 tagaataaaa cactttatga taatgggtat acctgctgtt gctgcaggtg tgtgtttggt
     2581 tattttttca gattacagaa gggataggat tatagctttt ctagatccgt ggaagtatcc
     2641 gagtaatgag ggatatcaag cgatacaatc cctttatgcc ataggttccg gcgggctttt
     2701 cggaagagga ctcggcaata gccttcaaaa gttcctctat attcctgagc cgtataatga
     2761 ttttatattg gcgatactgg cagaagagct tggatttatc ggggttgcaa cggtgcttat
     2821 actgtttctg atctttatat ggcgtggggt aaaggtggcc atgaatgcac cggatgcctt
     2881 tggaaccctg gtggcaacag gcataacctc attgattgcc gttcaggttg taatcaatgt
     2941 tgcggttgtt acatcttcca tgccggtaac cggaatgccc cttccgtttt tcagctatgg
     3001 gggttcttcc ttggttttcc ttttgaccaa tgtaggtata ctattaaata tatcaaagca
     3061 tgctaaatat gagagaatct gaggtgaatg gattttgaag gtacttatca gcgggggcgg
     3121 cacagcaggc cacattaatc cgggacttgc aatcgcaaaa tacataatga aaatggagcc
     3181 ggattcaaaa atattgtttg tgggtactca aaagggattg gagagcaaac tggttccgag
     3241 agagaatttc gatatagaaa tgataaaggt aaggggattt caaagaaagc tttcttttga
     3301 tactttggtt gcggtaaagg agttgtttca ggggcttttt gaggcaagaa aggttattcg
     3361 tgatttcaag cccgatgtgg ttataggtac ggggggatat gtgtgcggtc cggtggtgtt
     3421 taacgcttca aggatgaaaa tacccactct tgtccatgaa cagaatgctt ttcccggggt
     3481 gaccaataaa attctttcaa agtttgttga cagggttgca attagcttca aggaatcaac
     3541 aaagtatttt aagggaagaa aaaatgtgtt ttttacggga aatccgataa gaagtgagat
     3601 gcttgaagtc agtagggaag cagcccgtaa taagctcggt atacccaaaa gcgaatctct
     3661 tgtggtgata ttcgcaggga gcaggggagc agagaacgta aacagcaccg tttcagagat
     3721 gattaaaaag tacggaacgg atttcggttt tcatttgata tatgcaacgg gagaagccca
     3781 gtacagtggg attaaagaga gaattggcga tataaagtca aagaacgttc aggttttacc
     3841 gtacatatat gatatggcaa ataccatggc tgcagcggat ttggtggtgt gcagggctgg
     3901 ggcaattaca ataagcgagt tgactgcatt ggggatttct tctgtaatga ttccttcgcc
     3961 gtatgttacg gcaaaccatc aggagtataa tgcaaaggca ttggaacaac agggagcggc
     4021 agtggttatt ctggagaaga atttaagctg tgatatttta tataatcaga tcaatacact
     4081 tt
//
LOCUS       DAOOJC010000216         4078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_17087_length_4078_cov_2.990554, whole genome shotgun sequence.
ACCESSION   DAOOJC010000216 DAOOJC010000000
VERSION     DAOOJC010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17087_length_4078_cov_2.990554"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4078)
                     /locus_tag="PLA01_06685"
     CDS             complement(<1..>4078)
                     /locus_tag="PLA01_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HPD01022.1"
                     /translation="YQVSPAFIEHLAYRFRKMGKAYSHVLDYIDERLGESGTTVDQIT
                     HKEHNEQTVRKASIGNCIMSLKFISATNWVDIFERLNVVDKLLREDPDGSYPLLDLAT
                     RDYYRKSVEELAVKFSVSETHVAKKAVELAQRASQQQKEDNVRFSHVGYYLVGKGIKD
                     LEKEIGYEKKLIGRIAQRILSRPGILYMVSIGFIILVLIAGVVYYSLNNSSKYRWLLA
                     VISGLTVIIPATDIAVNAVNWIISRALKPSFLPKIEIKGDIPEDYAAMVVIPALLPDE
                     KRALELIDNLEVYYLANKDKNLYFSIAGDYKDAPKKEMPQDNKIIETALNRISELNKK
                     YGSGDRKVFYFFHRHRQFNDRQNKWMGWERKRGALLEFNEVLLGSKSTSYSIMSEELP
                     KLPKVKYVITIDADTILPLGAAKRLIGTMAHPLNRPVINKARGVVVEGYGLLQPRIEF
                     DIESTNKSLFSRIFAGEEGIDPYASAISDIYQDLFGEGIFTGKGIYDLEVFQELLNNA
                     IPDNTVLSHDLLEGSYVRTGLVTDVQLVDGYPSKVNSHAMRHHRWVRGDWQLLPWLKG
                     RIKDKSGSVIKNPLSLLTKWKIIDNLRRSMVAPSLMLLMFLGFGVLPGSPVFWFLSVL
                     LCMYFPFVTGCIDHVANKRFGTITLRKYAPVISGLRASFLQMTLQFILLPYNAYLMVN
                     AIVVTLYRVLITKKNMLEWVTALDTEKSLKNSLKGYIIKMKTAVFQSLVIVGLSVWLR
                     PDCLVLAVSLLAVWTLSPFIAYWVSKESVHKMKTLSEKEELELRRIARKTWRYFEEFM
                     NKRNNYLAPDNFQEDPPNGVAYRTSPTNIGLGMLAALTARDLGYIGTIELCDIVSRTM
                     DTIDRMEKWNGHLYNWYTTNTLETLRPRYISTVDSGNFVCYLITLKEGLLEYLKKPLI
                     DKSYVKGIRDTVSFINREKDESYSDTACLDQCFTDADGKSHGDAVLWAKALSELVENN
                     KSSGSSKDVWKLKVDNMIDMLRMEIYTYMPWANMLNDLPDGFEKIIGQPETKKLFEGI
                     REKLDADIPLIEMPEAYRDAVKKIDRLKKTAKSERNSDLTVNWFDELKKNLETAIDNS
                     ERLIERFNRLIERITKLSDGTEFIHLYDRKRQLFSIGYNIEENTLTDSYYDLLASEAR
                     QTSYIAIARGEVEPEHWFKLGRTLTQLDRYKGMVSWSGTMFEYLMPLLIMKSNRNTLL
                     DETYSFVVMGQKKYGRQRNVPWGTSESGFYAFDINLDYQYKACGVPWLGLKRGLIEDM
                     VVAPYATLLALPVDPEGVMDNIRRLVAEGAGGHYGLYEAIDYTPERVPIGEKKGNVKS
                     YMAHHQGMSILSLNNYLNGNIMQKRFHTDPVVEAARLLL"
BASE COUNT         1117 a         1003 c          714 g         1244 t
ORIGIN      
        1 aggagtagcc gggcagcttc caccacagga tcggtatgga aacgtttttg cataatgttg
       61 ccatttaaat aattatttaa agacaaaatg ctcataccct ggtggtgtgc catgtagctt
      121 ttgacattac ccttcttttc acctatcgga accctctcgg gtgtatagtc tatagcttca
      181 tacagtccgt aatgtccccc ggcaccttct gcaacaagtc ttcttatgtt gtccatcaca
      241 ccttccgggt ctaccggtaa agcaagaagg gttgcataag gagcaaccac catatcctca
      301 ataagccctc tctttagacc aagccatggc actccgcacg ctttatattg ataatccagg
      361 tttatgtcga atgcgtaaaa tccggattcg gacgttcccc agggcacatt tctttgcctt
      421 ccgtacttct tctgacccat caccacaaag gagtatgtct cgtcaagaag agtatttctg
      481 ttgcttttca tgataagcaa aggcataagg tattcaaaca tggttccact ccatgaaacc
      541 attcccttgt aacggtcaag ctgtgtaagg gtcctcccga gcttgaacca gtgctccggc
      601 tccacctctc ctctcgctat ggctatataa ctggtctgcc ttgcctcaga agccaataaa
      661 tcataatatg agtctgtaag agtattttcc tcaatattgt atccaatgga aaaaagttgc
      721 ctttttctgt catacaaatg gataaactct gttccgtcgg atagttttgt tattctctcg
      781 attaaacgat tgaacctttc aattagcctt tctgaattat ctattgcggt ttcaaggttt
      841 ttcttcagtt catcaaacca attcaccgtt aaatccgaat tccgttcgct tttagctgtt
      901 ttcttcagcc tgtcgatttt cttaaccgca tccctgtatg cttcaggcat ttctatcagt
      961 ggaatatctg catcaagctt ttccctaata ccttcaaaca atttttttgt ctccggctgt
     1021 ccgataatct tttcaaatcc atccggcaag tcgttgagca tgtttgccca aggcatataa
     1081 gtgtatatct ccattctgag catatctatc atattgtcga cttttaattt ccatacatcc
     1141 ttggaactgc ctgagctttt attgttctcg acaagttccg acaatgcttt tgcccacagc
     1201 actgcatccc catggctttt accgtcggca tcggtaaagc attggtccag gcaagcggta
     1261 tcgctgtaag attcatcctt ctcccggtta ataaagctaa cggtatccct tatccctttt
     1321 acatagctct tgtcgataag cggttttttg aggtattcca acaacccttc ctttaacgtt
     1381 atgagataac agacaaaatt tccgctgtcc acggtagata tatacctcgg tctcaaagtt
     1441 tccaaagtat tggtggtata ccaattataa aggtgtccgt tccatttctc catcctgtca
     1501 atggtgtcca tggttctcga cacaatgtcg caaagttcaa tagtacctat ataccccagg
     1561 tctcttgccg ttaatgccgc aagcataccc aatccgatat tggtgggaga agtcctgtaa
     1621 gctacaccat tgggagggtc ctcctgaaag ttgtccggag caaggtaatt gtttctctta
     1681 ttcataaact cttcaaaata tctccaggtc tttctcgcaa ttctcctgag ctccagctcc
     1741 tccttctcac ttaaggtctt catcttatga acactctctt tgctgaccca ataagctata
     1801 aagggcgata aagtccagac cgcaagcaat gaaacagcca acaccagaca atcgggtcta
     1861 agccagacgg atagtccgac aattaccaga gactgaaaca ccgccgtctt catttttatt
     1921 atatatccct tcagtgaatt cttaagggac ttttcagtgt ctaaagccgt tacccactcc
     1981 agcatattct ttttagtaat tagaacccta taaagagtca caacaattgc atttaccata
     2041 aggtatgcat tatatgggag gagaataaac tgcaatgtca tctgcaggaa agatgctcta
     2101 agcccgctga ttaccggtgc atatttcctc aaggttatag tcccgaagcg tttattagca
     2161 acatgatcta tgcatcccgt aacaaacgga aagtacatgc atagaagtac ggataaaaac
     2221 caaaaaacag gacttccggg caaaacgcca aaccctaaaa acataagcaa cattaaagaa
     2281 ggtgccacca tactccttct caagttgtct attatcttcc atttagtcaa taaagacaaa
     2341 gggtttttta tgacacttcc cgatttgtct tttatacgtc cctttagcca tggcaaaagc
     2401 tgccagtctc ctcttaccca ccggtgatgc ctcatagcat gggaattgac ctttgaaggg
     2461 tacccgtcaa ccaattgcac atctgttacc agtccggttc tgacataaga gccttctaaa
     2521 aggtcatggc tcaatacggt gttgtcaggt atggcattat tcaaaagctc ttggaacact
     2581 tcaaggtcat atataccttt tccggtaaaa ataccttcac cgaacaaatc ctgataaata
     2641 tcggaaatag cactggcata gggatctata ccttcttctc cggcaaaaat tctcgaaaat
     2701 agggatttgt tcgtactttc tatatcaaac tcaatccttg gctgcaaaag cccatatcct
     2761 tccaccacaa ctccccgggc cttattaata acaggccggt ttaaaggatg tgccatcgtt
     2821 ccgatcagcc ttttggctgc acccaaaggc aatatggtat cggcatcaat tgtaattaca
     2881 tactttacct tcggaagctt cggcagctct tccgacatta tcgagtagct cgtagatttc
     2941 gaacccaaaa gtacttcatt aaactccagc aacgcacccc tttttctctc ccatcccatc
     3001 catttgtttt gcctgtcgtt aaactgcctg tgtctatgga aaaaatagaa tacttttcta
     3061 tccccggagc cgtacttttt attaagctcg gaaattctgt ttaatgcagt ctcaattatc
     3121 ttattatctt gaggcatttc ttttttaggt gcatccttat aatcacccgc aatggaaaaa
     3181 tacagattct tgtctttatt tgccaaataa tatacctcaa ggttgtcaat aagctccaga
     3241 gcccttttct catcaggcag cagggctggt ataacgacca ttgcggcata atcctcagga
     3301 atatcaccct ttatttcgat cttgggcaga aaagatggtt ttaaagcacg gctgattatc
     3361 cagttaacag cattgacagc aatatccgtt gcaggaataa ttaccgtcaa cccggaaata
     3421 actgctaaca gccaccggta ctttgatgaa ttattcaacg aataataaac aactccggct
     3481 ataagcacca ggataataaa tccaatagaa accatgtata aaatgccggg acgggacaat
     3541 attctttgtg ctattcttcc tataagcttc ttttcatacc ctatttcttt ttccaggtct
     3601 tttatacctt ttcccacaag gtaatagccc acatgactga aacgtacatt atcctctttt
     3661 tgctgctgtg atgctctttg tgcaagttca acagcctttt tggcaacatg ggtttcagag
     3721 acactgaact tcaccgccag ctcttcaacg ctttttctgt aataatctct cgtagcaaga
     3781 tccaataaag gataagaacc gtcaggatcc tcacgcaata atttatcaac aacgttaagt
     3841 ctctcaaata tatccaccca gttggtggca gatataaact taagactcat aatacaattg
     3901 ccgatagatg cctttctcac ggtctgctca ttatgctcct tgtgagtgat ttgatcgact
     3961 gttgtaccgc tttcccccag tctttcatcg atatagtcaa ggacgtggga ataagctttt
     4021 cccatctttc taaacctgta agccagatgt tcaataaacg ccggactcac ctggtatt
//
LOCUS       DAOOJC010000217         4048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_17229_length_4048_cov_4.420486, whole genome shotgun sequence.
ACCESSION   DAOOJC010000217 DAOOJC010000000
VERSION     DAOOJC010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17229_length_4048_cov_4.420486"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..666
                     /gene="hisG"
                     /locus_tag="PLA01_06690"
     CDS             16..666
                     /gene="hisG"
                     /locus_tag="PLA01_06690"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPD01023.1"
                     /translation="MRYLTIALSKGRLTDMSVELFEAIGIDCTELKNSSRKLILSDEK
                     NKIKFFLAKPSDVPTYVEYGAADIGIVGKDTLLEEGRHLYEVLNLGFAACRMVVAGPA
                     ELMGKLDQLTNKRVATKYPRIAREYFEHKRKESIEVIKLNGSIELAPLVGLSEVIVDL
                     VESGRTLKENGLVVLDTIADISARMVVNRVSMKMESERINSIIEKLRKELEKRCED"
     gene            663..1961
                     /gene="hisD"
                     /locus_tag="PLA01_06695"
     CDS             663..1961
                     /gene="hisD"
                     /locus_tag="PLA01_06695"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010680945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPD01024.1"
                     /translation="MIKVIDLRNGKDSVLFETLASRSQMEHGDVLRRVEDIVNNVRQN
                     KDKAVLEYTAMFDKAELSSDTLKVTEDEIKEAYTKVDSRLVEVIKKSRDSIKSFHEKQ
                     KEKSWFVSEEDGVILGQMYTPLEIVGVYVPGGTAAYPSSVLMNIMPAKVAGVKKIVMT
                     TPPGKDGKVNPAILVAANEAGADEIYKVGGAQAVAALAFGTETIPKVDKIAGPGNIYV
                     AMAKRTVYGYCDIDMIAGPSEIMVVADETANPRFVAADLLSQAEHDVLASSILVTTSE
                     RVAKEVEKELGIQLEALDRKEIARKSIEDYGAIVIVDSIKDAAEVVNRIAPEHLELCM
                     EEPFGALGGIRNAGAIFLGSYSSEPLGDYFAGPNHVLPTSGTARFFSPLNLSDFMKKS
                     SIISYTKEALRKVKDDVILFAESEGLGAHANAIRVRFEKE"
     gene            1948..3084
                     /gene="hisC"
                     /locus_tag="PLA01_06700"
     CDS             1948..3084
                     /gene="hisC"
                     /locus_tag="PLA01_06700"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010680944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPD01025.1"
                     /translation="MRRSRDESQENLVQMREGACKMINDLVRPELRKLTPYVSHEVPF
                     RVKLDANESPFELPQKIREKLADYFVKGPGLNLYPDNESVSLRRALADYWSVDHDGIV
                     VGTGSNQLIQMIITVFVGKGDRVVIPCPTFSMYKINTIIAGGIPVEVPLLKEKDFALD
                     ADAFIDTVNKQGAKVAFLCNPNNPTGGLISIEDIEKVAAKCTGTIVVVDEAYAEFCEQ
                     SAIPLIEKYENLVALRTFSKAYLLAGARCGYSISNIAVSKEINKIRPTFNLSSLTQLI
                     AKMVFDEREEVEKMIGYLKDQREYLFEELKKFKNVYVYPSWANYILIRVQEAKEISCK
                     LYEKGVLIRSFSDDSALSDCIRVSVGTKEQNDIFLEELRSVMKY"
     gene            3255..3842
                     /gene="hisB"
                     /locus_tag="PLA01_06705"
     CDS             3255..3842
                     /gene="hisB"
                     /locus_tag="PLA01_06705"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HPD01026.1"
                     /translation="MNRKYETSRKTSETDISLSMNIDGTGKGDISTGVGFFDHMLNLF
                     AKHGLFDLKIRAKGDLDVDAHHTVEDVGIVLGQAIKEALGEKKSIKRYGSSFVPMDEA
                     LVLAALDLSGRPYLVFDGKLENEKLGNMDTELVEEFFRAVAFNAGINLHIKVFYGSNT
                     HHIIEAMFKAFGRALDEASGMDNRIEGVMSTKGML"
     gene            3901..>4048
                     /locus_tag="PLA01_06710"
     CDS             3901..>4048
                     /locus_tag="PLA01_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPD01027.1"
                     /translation="MLIGDTNFINLPLHIRGKVRNVYDLGDKLLIVVTDRISAFDVVF
                     PNLIP"
BASE COUNT         1253 a          671 c         1068 g         1056 t
ORIGIN      
        1 taaggagggt aggatatgag atatttgacg atagcccttt caaagggaag gcttaccgat
       61 atgtcggtgg aactgtttga ggccataggg attgattgca cggagcttaa gaattcatcc
      121 cggaaactta ttttaagtga tgaaaaaaat aaaatcaaat tctttctggc aaaacccagc
      181 gatgtcccca catacgtgga atatggggct gcggatatag gtattgtggg taaagataca
      241 cttttagagg aaggcaggca tctttacgag gttctaaacc ttggatttgc tgcctgcagg
      301 atggttgttg cgggaccggc ggaactaatg gggaagcttg accaacttac caataaaaga
      361 gtggcaacga aatatccgag gattgcgagg gagtactttg aacataaacg caaggaatcc
      421 attgaggtca tcaagctcaa tggctccatt gagctggcac ctttggtggg actttccgag
      481 gtgatagtcg acctggtgga gagcggcaga acattaaagg aaaacggcct tgttgtcctg
      541 gataccatag cggatataag tgcaagaatg gttgtaaaca gggttagtat gaagatggag
      601 agtgagcgta taaactccat aattgaaaaa ctgagaaaag agttggaaaa gaggtgtgag
      661 gattgataaa ggttatagat ttgagaaacg ggaaggacag cgtattattc gaaaccctcg
      721 catcaagaag tcagatggag catggtgatg tattaagacg tgtggaggat atagtaaaca
      781 atgtaaggca gaataaagat aaagcagtat tggaatacac tgctatgttt gataaggcag
      841 agctttcttc tgatacccta aaggttacag aagacgaaat aaaggaagcc tacacaaaag
      901 ttgattcaag actggtggaa gtaattaaga aatccaggga cagtataaaa agctttcatg
      961 aaaagcagaa ggaaaagtca tggtttgttt ccgaagagga tggagtaatc ttagggcaga
     1021 tgtatacgcc tttggagata gtaggtgttt atgtgcccgg aggaacggcg gcttatcctt
     1081 catcggttct tatgaatata atgcccgcaa aggtagctgg agttaaaaag atagtaatga
     1141 caacacctcc gggaaaggac ggaaaggtga accctgccat actggtggct gccaatgaag
     1201 ccggtgccga tgaaatatac aaagtgggcg gggcccaggc tgtagctgca ctggctttcg
     1261 gtaccgaaac aatacccaaa gtcgataaga ttgcaggacc gggtaatata tatgtggcaa
     1321 tggcaaaacg gacggtatac ggttactgtg acattgatat gattgcaggt cccagtgaaa
     1381 ttatggtggt tgccgatgaa actgcaaacc caaggtttgt ggcggcagat cttctgtccc
     1441 aggccgagca tgatgtgctg gcttcgtcca tattggtcac cacgtcggaa agggttgcaa
     1501 aagaggtgga aaaagagctg ggaattcagc tagaggcttt ggacagaaaa gagatagccc
     1561 gaaagtctat tgaggattat ggtgctattg ttattgtaga cagcattaaa gatgctgcgg
     1621 aggtagtaaa ccgaattgct cccgagcatc tggaactctg catggaagaa ccctttggtg
     1681 ccttgggcgg tataaggaat gcgggggcaa tatttttagg gagttattcc tcagagcctt
     1741 tgggggatta ttttgcagga ccgaatcatg tgcttcccac cagcggtaca gcaaggtttt
     1801 tctctccctt gaacttatcg gactttatga agaagagcag cattatctcc tatacaaagg
     1861 aagcactccg taaggttaag gacgatgtaa tattgtttgc cgagtcggaa ggactgggtg
     1921 cacatgcaaa cgccatcagg gtgaggtttg agaaggagta gagatgaaag ccaagagaat
     1981 ttggtacaga tgagagaagg tgcatgtaaa atgataaacg atttggtgag gccggagctt
     2041 cgaaagctta ctccgtatgt ttcccatgag gtacccttca gggtgaagct tgatgccaac
     2101 gaaagcccct ttgaacttcc gcaaaaaata agggagaagc tggccgatta ttttgtgaag
     2161 gggccaggct tgaatttata tcccgacaat gaatcggtta gcctgaggag ggcacttgca
     2221 gattactgga gtgttgacca tgacggcatt gtagtgggaa ccggatccaa ccagcttatc
     2281 caaatgataa taacggtgtt tgtcggaaaa ggtgacaggg tggttattcc ctgtcccacg
     2341 ttttcgatgt ataagataaa taccataata gccggaggta taccggtgga ggttcctctt
     2401 ttgaaggaga aggattttgc ccttgatgcg gatgctttta tagacaccgt aaacaaacaa
     2461 ggggcaaagg tggcgttttt gtgtaatccc aacaacccta cgggcggatt gatttctata
     2521 gaggatattg aaaaagttgc agcaaagtgc accgggacaa tcgttgttgt tgatgaggcc
     2581 tatgccgagt tttgcgaaca aagtgcaata cccttaattg aaaagtacga aaaccttgtg
     2641 gcgcttagaa ccttttccaa ggcttacctt cttgcgggag caaggtgtgg ctattcaata
     2701 agcaatattg ctgtttcaaa ggagataaat aagatccgtc ctacattcaa cttaagctca
     2761 ctgacccagc ttattgcaaa gatggtcttt gatgagcgtg aagaagttga aaagatgatt
     2821 gggtatttga aggaccagag agaatacctt tttgaggagc ttaaaaagtt taagaatgta
     2881 tatgtttatc cgtcatgggc aaattacata ctgataaggg ttcaagaggc aaaagaaatc
     2941 agctgtaagc tttatgaaaa gggagtgttg ataagaagct tttccgatga ttccgcactt
     3001 tccgactgta tcagggtaag tgtgggcaca aaagagcaga atgatatctt tcttgaggaa
     3061 cttagaagtg ttatgaaata ttaaaatgaa agaaatcttt tgcaaaggga aattattctt
     3121 tggactcaag tgttatagta ggacattaat ttttataaac ttattgttgc gattttctat
     3181 agaaatggta gaataaaaat agtttacgtg tagattaaag ggtaaggatc attacatact
     3241 tcggaggcat ttggatgaat agaaagtatg aaacaagcag gaaaaccagt gaaaccgata
     3301 taagcctcag catgaatatt gacggcacgg gaaaaggtga tattagcacc ggagtgggct
     3361 tttttgacca tatgctcaat ctgttcgcca aacacggtct ctttgacctt aagatcaggg
     3421 caaagggaga cttggatgtg gatgcccatc atactgttga ggatgtcgga attgtattgg
     3481 gacaggctat aaaagaggca ttgggagaga aaaaatccat aaaaagatac ggttcttcct
     3541 ttgtccccat ggacgaagcc ttggtactgg cagcccttga cctgagcggc agaccatatc
     3601 tggtgtttga cggaaaactg gaaaatgaaa aacttggcaa tatggacacc gagcttgtgg
     3661 aggagttctt cagggcagta gcctttaatg ccggtataaa cctgcatata aaagtttttt
     3721 acggcagcaa tacccaccat attattgagg ctatgttcaa ggcttttggc agagcccttg
     3781 atgaagcatc ggggatggac aatagaattg aaggtgttat gtccactaag ggaatgcttt
     3841 aagatggaat attactacac acgtattcaa tagaaatcta tgtaaaagag gtgctgagta
     3901 atgttaattg gagatactaa ttttataaat cttcctttac atattagagg taaggttaga
     3961 aatgtatatg atttgggaga taaactgttg attgttgtta ccgacagaat atctgccttt
     4021 gatgtggtgt ttccaaacct tataccca
//
LOCUS       DAOOJC010000218         4026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_17355_length_4026_cov_3.694284, whole genome shotgun sequence.
ACCESSION   DAOOJC010000218 DAOOJC010000000
VERSION     DAOOJC010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17355_length_4026_cov_3.694284"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(439..1494)
                     /locus_tag="PLA01_06715"
     CDS             complement(439..1494)
                     /locus_tag="PLA01_06715"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPD01028.1"
                     /translation="MKKLAIILIVSVMIAVLNVTGFASGNSYSGYWSIPSDAIIAVEG
                     TIINPERQPITIDGRILVPARSTFEMIGVRTDWYPEHEVVKMVKGNNYITMSMGGKSA
                     YVNGTVKNMEAPAVLVDGTVMVPIRFVAENFDSSVGWDGKNRMAYIGYAAELPVRGGG
                     LGSDRVYKVVIDAGHGGSQSGAVYGGVKEKDLNLDIAKRLNALLKKEGIQTYMTRDKD
                     STVSLYSRSGLANNVGADLLVSIHNNAGQSKASGTLTLYYPGSQKSKGNLSSYEFAKI
                     VQMNLNKELGTKDMGVIERPNLAVLRTSNMPAVIAEIGYMSNSAELSKLKTDTYRQNA
                     AEALKNAVLQSLNKMYH"
     gene            1750..1875
                     /locus_tag="PLA01_06720"
     CDS             1750..1875
                     /locus_tag="PLA01_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01029.1"
                     /translation="MPDRIGRSIKYKCDIETTSLLWSNDVILITPVKNKKAIPVI"
     gene            complement(2367..3272)
                     /locus_tag="PLA01_06725"
     CDS             complement(2367..3272)
                     /locus_tag="PLA01_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPD01030.1"
                     /translation="MGRDGYNSKTSKGKSVGYFASIKRKKKIKGIFANIVLAIMVVFS
                     LGPILYMIYCSLVHHNVVAQGKLAWPIKFSNYVEVFQKLPFGTYFRNSLIVCLCVMVI
                     ALIIATLAGYSLAKYKFPGSGVFGILVLSTQMLPGMMFLLPLYLQFIQIQEKTGIQMT
                     NTLHGIIIIYSAFYVPFSIWIIRGFFASIPGELEEAARIDGCNKFTAFLRVMLPLAVP
                     GIIATAIYIFLMAWDELLFAQVLLQKADVFTIPVGIRAFIAHTIARYDLLMAAGSVVT
                     VPVLIMFFSMQKKFISGMTAGAVKG"
     gene            complement(3272..>4026)
                     /locus_tag="PLA01_06730"
     CDS             complement(3272..>4026)
                     /locus_tag="PLA01_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPD01031.1"
                     /translation="DNYYEVLFNKNSLIRTGFWQALRNTGIYTFTVTLGTLTFGLIIA
                     MLVNREFKGKGLVRTALLMPWVVPSYVVGIVWGFMWFQDIGLVNRVFHDILHIFPTKP
                     YWLVGFNQIFAIIIPTIWRGLPMSMILMLAGLQSISPDYYEAAEIDGANGWQQFWNIT
                     LPLLKPILSINIMFSLIGNIYSYNIVSMMFGNGAGIPGEWGDLLMTNIHRNSFQLWSF
                     GPGAAAMMIVMFFVMIIVVLWYLFFKDDLVVK"
BASE COUNT         1347 a          851 c          605 g         1223 t
ORIGIN      
        1 cctatattga tgacataaag gcatttgaaa acaatccgga ctggcccatt tacaaaaagc
       61 aacatataaa acgggccttg aaagaatata acaacctcta caaagaagta caaaacgata
      121 gaatggatcc ggatttcctt cacgaaaaca tcacaagctt tgtttatatg ctgcagtaat
      181 gttttgataa cttcataatt actgtgaaat atttttatca atgcatattg cattttaatg
      241 tgttgtggag gatttggcca tttacttcat aaaattcctc caaaaataaa acccagggac
      301 acttaacccc tgagtttttt agcctttcat acactatgca ttatcatagt tttctcaaat
      361 atttaatcga ttaaacctct acgaacttat cgagccgtca agacttttaa atttttacgt
      421 aagtaatttg agaacactct aatggtacat tttattaagg ctttggagaa ctgcattttt
      481 taaggcttct gccgcattct gcctgtaagt atctgttttt aacttagata gctcagcaga
      541 attgctcata taacctatct ctgcaattac tgcaggcatg ttcgaagtgc gtaacaccgc
      601 cagattcggc ctttcaatta ctcccatatc ctttgttccc agttccttat tcaagttcat
      661 ctgaactatt ttggcaaatt cataggaaga gagatttccc tttgatttct gactgccggg
      721 ataatatagt gtcaaagtgc ccgatgcttt gctttgacct gcattgttat ggatgctgac
      781 cagaagatca gcaccgacat tgttggcaag tcctgatctt gaatacaaac ttaccgttga
      841 atctttatct cttgtcatgt atgtttgtat tccttccttt ttgagcagag catttaatct
      901 ttttgcaatg tcaagattca ggtctttctc ctttacacca ccatacacag cacctgattg
      961 gctgccaccg tgtccggcat caataacaac cttataaact ctgtcagaac ctaatccacc
     1021 acctcttacc ggcaactctg ctgcatatcc gatataagcc attctgtttt ttccatccca
     1081 tccgacagaa gaatcgaaat tctcggcaac aaaccttatg ggaaccatga cagtcccatc
     1141 gacaagcact gccggtgctt ccatattctt cacagtcccg ttcacataag cagatttgcc
     1201 tcccatactc atggtaatgt aattatttcc ctttaccatt tttacaactt catgttcagg
     1261 ataccaatct gtcctgacac ctatcatttc aaaagtggat cttgccggaa caagtattct
     1321 tccatcaatg gtaatcggct gcctttccgg atttataatc gtaccctcca cagctattat
     1381 tgcatccgat ggtatactcc aatatcctga ataactattg cccgatgcaa aacctgtaac
     1441 attcaaaact gctatcatta cagacactat cagtattata gccagctttt tcatctcttc
     1501 ccccctctca tatttattgg tttacataaa attataccac ataaaaatac aatatccttt
     1561 atgtaatttt tgttatctca aaggactaaa taaatataaa agtaataaat agatatattt
     1621 attgtttcac tgcatatttc tgcatacaat ataactaaag agggaaacta cattatttaa
     1681 tttctgaact tccttcgaac aaagggaaat atccagaacc caagggacat cgaaacaagg
     1741 caggcactaa tgcctgatag aattggaaga agcataaaat acaaatgtga cattgaaaca
     1801 acttcgctgc tttggagcaa cgatgtcatt ttaataacac ctgttaaaaa caagaaagct
     1861 attccggtaa tctgaaaaac atagtgctgt ttaaatagat tcaatataga tgatgataat
     1921 aaaaccacaa taaatactgc tattgaatat aatatactag aatccaaatt ccaacacccg
     1981 gtctttcaat ttttgtaata ttaacaattc attcttgcca tcaattattt aattatattg
     2041 taatagcata ttaaaaataa ttcaaactat ttccctgaac aggagtgtgt ttaatgttct
     2101 gggtgtatcg aaccattatc gcttttggac atttcacttt aaaaaattcc gtgaacaaaa
     2161 tttcagatgg catcgagccc ccatcgcttt ttggtcattt cacttcgtga aatcccacta
     2221 aaaaatatgg actcggatag ggaatacacc ctctctccga gtccataaaa tattcttaaa
     2281 cttattgcta cttagatgag caaactctat aaaccttatc ggcaagttta ctctaaacgt
     2341 acatttgctg aaatcatgtt caaaaattaa cccttaacag ctccggcagt cataccgctt
     2401 ataaacttct tctgcattga gaagaacatt ataagaacag gtacagtaac aacagaacct
     2461 gctgccatca agagatcgta tcttgctatg gtatgagcaa taaacgccct tattccaacc
     2521 ggaatagtga aaacatctgc tttttgtaaa agaacctgtg caaaaagcaa ttcgtcccac
     2581 gccattaaga atatataaat tgcagttgca ataatccccg gtacagccag aggcagcatt
     2641 acacgcaaga atgcagtaaa cttgttacag ccatctattc tcgcagcctc ttcaagttct
     2701 ccaggtattg aagcaaaaaa tcctcttatt atccatatac taaacggtac atagaatgcg
     2761 gaataaatga tgataatacc atgaagtgta ttcgtcattt gaattccggt tttttcttgt
     2821 atctgaatga actgtagata aagaggaaga aggaacatca ttcccggtaa catctgagtt
     2881 gacaaaacca atataccgaa aacgcctgaa cctggaaatt tatacttagc aagagaataa
     2941 cctgctaaag tagcaattat aagtgctatt accattacac ataaacatac tataagactg
     3001 ttcctgaaat atgttccaaa aggtaatttt tgaaacactt caacatagtt ggaaaacttt
     3061 ataggccatg ccagctttcc ttgtgcaact acattatgat gtaccaatga acaatatatc
     3121 atatataaaa taggtcctaa agaaaaaaca accataatag caagaacaat attggcaaaa
     3181 atacccttaa tttttttctt tctttttatt gaagcaaaat aaccaacact tttacctttg
     3241 cttgtttttg agttgtaacc atctcttccc attatttcac caccaaatca tctttaaaga
     3301 ataaatacca taataccaca attatcatta caaagaacat tacgatcatc attgcagccg
     3361 caccagggcc aaatgaccat aattgaaatg aatttctgtg gatattggtc attagtaagt
     3421 ctccccattc accgggaatt ccggcaccat taccaaacat cattgaaacg atgttgtatg
     3481 agtaaatatt acctataagg gagaacataa tattaataga aagaatcggt ttaagcaaag
     3541 gcaaagttat attccagaat tgttgccaac catttgcacc atctatttca gcagcctcat
     3601 agtaatcagg agatatgctc tgaagtcctg ccaacatcaa aatcatggac atcggcaatc
     3661 ctctccagat tgtcggaatt attattgcaa atatctgatt aaatccaacc aaccaatagg
     3721 gctttgtcgg aaatatgtgc aatatgtcat ggaatacacg gttaaccaaa ccgatatcct
     3781 gaaaccacat aaagccccaa acaataccga caacatatga aggaacaacc catggcataa
     3841 gaagtgctgt tctaacaaga ccttttcctt taaactccct gttaacaagc atggctataa
     3901 taaggccaaa ggtaagagta cccagagtaa cagtgaaagt ataaattccg gtatttctaa
     3961 gagcctgcca gaaaccggtt cttattaagc tgtttttatt aaaaagtact tcgtagtagt
     4021 tatcaa
//
LOCUS       DAOOJC010000219         4006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_17457_length_4006_cov_3.536320, whole genome shotgun sequence.
ACCESSION   DAOOJC010000219 DAOOJC010000000
VERSION     DAOOJC010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17457_length_4006_cov_3.536320"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..702)
                     /locus_tag="PLA01_06735"
     CDS             complement(133..702)
                     /locus_tag="PLA01_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPD01032.1"
                     /translation="MSLFDGESDWKDRLNIAQAKPWVRFWARTVDLYIVDVIIKAIQL
                     ILFQGKLFNPLLLGFGSSLIWAFAEANSIYTWGTTPGKWLLRTEVRANNLKKPDLFAS
                     IKRSILVWLLGVGMGVFSSFTYIAGYYLLTRKGYTLWDKYSDCYVVHKKMSDNRAIVA
                     VFIIIALVIVDFAFSFVNAYFYCKEAGLI"
     gene            complement(745..1296)
                     /gene="scpB"
                     /locus_tag="PLA01_06740"
     CDS             complement(745..1296)
                     /gene="scpB"
                     /locus_tag="PLA01_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPD01033.1"
                     /translation="MDLKKLEGIIEGILFAAGDRVSMERICSIIGIDKKTVKLVINNM
                     IVNYNNNPSRGISIREINNGYQMCSKPEYYDYLKLLFEPKQKTGLSQAALETLAIIAY
                     NRPITKAKIENIRGVNSDSAVARLLERNLIKEAGKLDAPGKPILYETTDEFFRSFGFK
                     GDSDLPIFELNEMQEQPDNNSEE"
     gene            complement(1314..2108)
                     /locus_tag="PLA01_06745"
     CDS             complement(1314..2108)
                     /locus_tag="PLA01_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPD01034.1"
                     /translation="MESALSKACTIKIQNFEGPFDLLFHLIEKNKINIYDIPINEITD
                     QYMDYLFKMQELDMEVASEFLVMAATLLHIKSRLLLPNPRQDEKEEIDPREELVLKLV
                     EYKRYKRFTEILKQREQEGDKYFYKGPEDIDIKHVDEPIELSYDEIKRVYLELLERNE
                     KKMNKNTGKMTQIIQTEKVSLRSKIKEVINTLLKRSFFRFSELFSPKTKSRLEIVTGF
                     LAILELTKLKKITITQQKQFSEIMVYKRDDTNLEDTDKNKIAAENS"
     gene            complement(2153..3142)
                     /gene="trpS"
                     /locus_tag="PLA01_06750"
     CDS             complement(2153..3142)
                     /gene="trpS"
                     /locus_tag="PLA01_06750"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPD01035.1"
                     /translation="MKKGKILSGMRPTGVLHLGNYFGALENWVKLQDEYDCYFFVADW
                     HALTTGYEDTSEIKNNINNIIIDWISAGLDPERCVMFLQSSVKEHAELHLLFSMTTPL
                     SWLYRCPTYKDQLNQIKDRNISTYGFLGYPCLQAADILMYKAGFVPVGEDQLPHLELT
                     REIARRFNNLFEEVFPEPQALLTKAKVLPGTDGRKMSKSYNNTIALSDSPDEVRKKVS
                     TMITDPARIRKDDPGHPEVCTVFAFHKVFNEEEIPQIEEDCKGGKIGCVQCKKKLAQK
                     MAEYLEPIYERRQKLLEKPGYVKEVLQNGNENARKVAGKTIEEVKKAMKIDFM"
     gene            complement(3289..3993)
                     /locus_tag="PLA01_06755"
     CDS             complement(3289..3993)
                     /locus_tag="PLA01_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPD01036.1"
                     /translation="MIFDLLKTPELIIYWFMIFAFSISFHESAHAYVAYRMGDSTAKE
                     QGRITLDPLKHLDLFGTIMILISFIGWAKPVPIDPRNFKNRKQGTILVSLAGPVSNLI
                     LAILFSIPLIFITYMFYPDAVNGIKTTLTPWFGASLDPRQIIFNFCSFGVFMNISLAI
                     FNFLPIPPLDGSKILTGVLPAKYYFKIMDYQQVSFIILIILSYTGWLGNIIIPVIVWV
                     RAAIFFIIELLVKLIM"
BASE COUNT         1176 a          855 c          656 g         1319 t
ORIGIN      
        1 atatagtgtt agtttgtcat gacatgagtt atataatgaa gtatattaat gcattctatc
       61 tgaccgaagt tcggataaaa ggtacataca aaaatatctt atactgcttc taagtaatat
      121 atcaaagcta ttttaaatca gcccggcttc tttgcaatag aaatacgcat ttacaaaact
      181 gaatgcgaag tcaacgataa ccaaagcaat aataataaat actgcaacaa tggccctgtt
      241 atcgctcatt tttttatgga ctacatagca atcgctgtat ttgtcccata gtgtataacc
      301 cttgcgtgtc aataagtaat agccggctat ataggtaaaa cttgaaaaaa cgcccattcc
      361 tacgccaagt aaccaaacaa gaatacttct cttaatagag gcaaaaagat ccggcttttt
      421 taaattattt gcccttacct ctgttctaag caaccatttc cccggtgtag ttccccatgt
      481 ataaattgaa ttggcttcag caaaagccca aatcaaagag ctgccaaagc ccaaaagcaa
      541 agggttaaaa agcttgcctt gaaaaagaat caattgtatt gcttttatta ttacatcgac
      601 aatatacaag tcaacagttc gtgcccaaaa ccttacccaa ggcttagcct gggctatatt
      661 caaacggtct ttccaatcgc tttcgccatc aaataggctc atttaaatca cctgaattac
      721 aaatatttta aaaatttgtg ttaattactc ttccgaatta ttgtccggtt gttcttgcat
      781 ttcattcaac tcaaatatgg gtaaatccga atcaccctta aaaccgaagc ttctgaaaaa
      841 ctcatcggta gtctcataca atatgggctt acccggtgca tcaagttttc cggcctcttt
      901 tatgagattg cgttctaaaa gccttgcaac ggcactgtcc gaattcacgc ccctgatatt
      961 ctctattttt gcttttgtta ttggtctatt atatgctata atagccagag tttcaagtgc
     1021 tgcctgagac aaaccggttt tctgtttcgg ttcaaacagc agttttaaat aatcataata
     1081 ctcgggtttg gaacacattt gataaccgtt gtttatttct ctaatgctaa tccccctcga
     1141 agggttatta ttgtagttta caatcatgtt attaataaca agcttaactg tttttttatc
     1201 aataccaatt atagaacaaa tcctttccat ggacaccctg tcccctgccg cgaaaagaat
     1261 cccttcaata attccttcaa gttttttcaa atccatataa ccactccagg catctatgag
     1321 ttttccgctg caatcttatt tttatcggta tcctcaagat tagtatcatc ccgcttgtag
     1381 accattattt ccgaaaactg tttttgctgc gtaatcgtta tttttttaag cttcgtcagc
     1441 tcaagaatgg ccagaaatcc tgttacaatc tcaagtctcg actttgtttt gggagaaaag
     1501 agttccgaaa acctgaaaaa tgaccttttc aaaagcgtat ttattacctc ttttatttta
     1561 cttcgcaaag ataccttttc agtttggatt atctgagtca ttttgccggt gttcttgttc
     1621 attttctttt cattacgttc caacagctcc aaataaactc tttttatctc gtcataagag
     1681 agctctatag gctcgtccac atgcttgata tcaatgtcct caggtccctt ataaaagtac
     1741 ttatcgccct cttgttccct ttgctttaag atttcggtaa atctcttgta tctcttatat
     1801 tcaacaagct ttaaaaccaa ctcttccctc gggtcaatct cctctttttc atcctgacgc
     1861 ggattcggaa gcaaaagcct agatttaatg tggagaagag tagcagccat gacaagaaat
     1921 tcgctggcaa cctccatatc aagctcctgc atcttaaaga ggtaatccat gtattgatcg
     1981 gttatctcat ttataggtat gtcatatatg ttgattttgt ttttttctat cagatgaaac
     2041 aacaaatcaa aaggaccttc aaagttttgt atttttattg tacatgcttt tgacaatgca
     2101 ctttccaatt atatctacca cccttgtcag gtaccaatta tattaaaatg gtttacataa
     2161 aatcaatttt catagccttt ttaacctctt caatagtctt gccggctact tttctggcat
     2221 tctcattacc gttttgcaaa acttccttta cataaccggg tttctccaga agcttctgcc
     2281 ttctttcgta aataggctct aggtattccg ccattttttg ggcaagtttc ttcttacact
     2341 gcacacagcc gattttacca cccttgcaat cttcctcaat ttgaggtatc tcctcttcat
     2401 taaatacctt atgaaaagca aatactgtac acacttcggg atgtccgggg tcgtccttcc
     2461 ttatcctggc tgggtcggtt atcatagtgc tgactttttt cctgacttca tccgggctgt
     2521 cggatagagc tattgtgttg ttatagctct tgctcatttt tcttccgtcg gtacctggca
     2581 gtaccttagc ctttgtaagg agtgcctggg gctcagggaa tacttcctca aaaaggttgt
     2641 taaaacgcct tgcaatctcc cttgtaagtt caagatgcgg cagttgatcc tctcccaccg
     2701 gtacaaatcc tgccttgtac atcaatatat ctgctgcttg aagacatgga tagcctaaaa
     2761 atccataggt tgaaatattt ctgtccttaa tctggttaag ctggtccttg taggttggac
     2821 agcggtaaag ccatgacagt ggtgtggtca tggaaaacaa aagatgcagc tcggcatgtt
     2881 ccttaacact cgattgcaaa aacataacac acctttcggg atcaagacct gcacttatcc
     2941 aatctataat aatgttattt atattattct taatttcaga agtatcttca tatcctgttg
     3001 tcagggcatg ccaatcggcc acaaaaaaat aacaatcata ttcatcctga agctttaccc
     3061 agttttccaa ggcaccgaaa taatttccca ggtgcaatac acctgtcggc ctcatgccgc
     3121 tcagtatctt tccttttttc actgaaaatt cactctccca atctttattg ccggatttaa
     3181 taaaatcaca ttattaaatt atatcttttt ttttcaataa agtctaattt tatttttcag
     3241 atggatgcat ttattatatg cttcacaatg tttcttacgt atttttcttt acataatcaa
     3301 tttaaccaat agttcaatga tgaaaaaaat tgcagctctt acccatacta taaccggaat
     3361 gatgatatta ccgagccaac cggtatagga caggataatc agtattataa aggaaacctg
     3421 ctggtaatcc attatcttga aataatactt agccggtaaa acacctgtaa gtatcttgga
     3481 gccgtccaaa ggcggaatcg gcagaaaatt gaatatagcc agacttatat tcataaatac
     3541 accgaaggaa cagaaattga agattatttg cctgggatca agagaagcac cgaaccaagg
     3601 cgttaatgta gtcttaatcc catttaccgc atcaggataa aacatgtagg taataaaaat
     3661 aagaggaata gaaaaaagta tcgcaagaat taagtttgat accggacctg ccaggcttac
     3721 aagtatggta ccttgctttc tgtttttgaa gtttctgggg tcaatgggta ccggcttggc
     3781 ccatccgata aagcttatca atatcataat tgttccaaac aaatccaggt gctttaaagg
     3841 gtcaagggta attctcccct gttcttttgc cgttgagtcg cccatgcggt atgcaacata
     3901 agcatgggcc gattcgtgga acgaaatgga aaaagcgaaa atcataaacc aatatattat
     3961 taattcagga gttttcaaaa ggtcaaatat cattttcttc tccttt
//
LOCUS       DAOOJC010000220         3990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_17545_length_3990_cov_3.679288, whole genome shotgun sequence.
ACCESSION   DAOOJC010000220 DAOOJC010000000
VERSION     DAOOJC010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17545_length_3990_cov_3.679288"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(471..2573)
                     /locus_tag="PLA01_06760"
     CDS             complement(471..2573)
                     /locus_tag="PLA01_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPD01037.1"
                     /translation="MNNNNINITEIFGSLVFNDRVMRQRLPKDTYNALKRTIEMGKSI
                     DRSIADTVASAMKDWAIEKGATHFTHWFQPMTGITAEKHDSFISPLQDGSIILEFSGK
                     ELVKGEPDASSFPSGGLRATFEARGYTAWDPTSYAFIKDSTLCIPTVFYSYGGEALDK
                     KTPLLRSMDALNKQALRIMKLFEHDNVTRIITTVGPEQEYFLVDKGLFEKRKDLIYCG
                     RTLFGAKPPKGQELEDHYFSTIKPRVSAFMKELDEELWKLGVFVKTKHNEVAPAQHEL
                     APIFTTTNIATDHNQIVMEIMKTVASRHGLTCILHEKPFAGVNGSGKHNNWSLSTNTG
                     INLLEPGDSPMENAQFLLFLVAVIAAVDDYQDLLRLSVASAGNDHRLGANEAPPAIIS
                     VFLGDEITGILEAIQNGSSYDKKDRIEIKLGVDVLPYIPKDTTDRNRTSPFAFTGNKF
                     EFRMPGSALSISGPNIVLNTIVAEKLSLFADTLEKSSDFKNDLNSLIKETVKKHKRII
                     FNGNNYSDEWVAEAEKRGLSNLKTTADVLPCFISQKSIDLFTKHNVFSEVEIHSRYEI
                     LLENYCKTLNIEVLTMIDMVRKDILPAVMRYVKDLTKMALSKKELDMEIPCEPERNLI
                     HKLSSLSARLYENTNELENKLLDAKSYTDMQAQANYIRDIIIPIMQKVRTAADELEVL
                     TSSEYWPFPTYGELLFKV"
     gene            complement(2622..3629)
                     /gene="asnA"
                     /locus_tag="PLA01_06765"
     CDS             complement(2622..3629)
                     /gene="asnA"
                     /locus_tag="PLA01_06765"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HPD01038.1"
                     /translation="MSKTVIPSGYRSALDLYQTQKAIAFLKENFQKRLCKALNLVRVS
                     APLFVDPSTGLNDDLNGIERPVSFDIAETQTVAQVVHSLAKWKRMALYKYGFAPGEGL
                     YTDMNAIRRDEELTNLHSVYVDQWDWEKIITPETRNLSYLKETVCKIVGAVCDTLDDL
                     KKSYPQIEVSLEREVSFVTTQELEDMYPALSPKQREDEYLKGYKTAFIMQIGDLLKSG
                     QKHDGRAPDYDDWSLNGDIVFYSELLGRAFEVSSMGIRVDAKALDEQLTKANCDYRRA
                     LSFHQMLLNGTLPLTMGGGIGQSRLCMLLLGKAHIGEVQVSIWDKDTIAACEDAGISL
                     L"
BASE COUNT         1072 a          827 c          864 g         1227 t
ORIGIN      
        1 ggaagaaaac ctctgcttcc tattaaggtt ataaaatcca aaattattta tgagtcttaa
       61 ttaaactaca gtgtagtaac atgaaaatat gaaaaatatt gactaaaaac aattaatatg
      121 ctaagattaa attgatggag gaactatagt gtctgtacca tcaaacaccg caggaatttt
      181 ttctatgcgg ttttttattt taatgagtat taattattat aacatagaat ataataaaaa
      241 tgatagagga ttttatctgt actatcaaac cctataggat aaattcatat aggataaata
      301 aaaatgggct attgcactaa gaaattagtg cagtagctct ttataatgtt taggaaagtt
      361 cgtactattg gaaattataa gaataagcgg aacagtaaaa aaatcccacc aatgaagccc
      421 caccaatgaa gggaaatggt ggggctgggg ggttttttat accgcacgca ctaaaccttg
      481 aaaagaagct cgccataagt cggaaacggc cagtattccg aagaagtaag tacctcaagc
      541 tcatcggctg cggtacgtac cttctgcatg atggggatta ttatatccct gatataattg
      601 gcctgtgcct gcatgtcggt atagcttttt gcatccagca gcttgttctc caattcattt
      661 gtattttcat aaagccgggc tgagagagac gacagcttgt gaatcaaatt ccgctcaggc
      721 tcgcagggta tttccatgtc cagttctttt ttggacagtg ccattttggt taaatccttt
      781 acgtatctca tgaccgcggg aagaatgtct tttcttacca tatcaatcat tgtaaggact
      841 tcgatgttca gtgttttgca gtagttttcc aaaaggatct catagcggga gtgtatttca
      901 acttctgaaa acacgttatg ctttgtaaag aggtctatgc ttttttggct gataaagcag
      961 ggcagaacat ccgctgtggt tttcaggttt gaaagtcccc tcttttcggc ctctgctacc
     1021 cattcatcgc tgtaattgtt tccgttgaat atgattcttt tatgcttctt gacagtttct
     1081 ttaataagcg agttaaggtc gtttttaaag tcagaggatt tctccagggt atcggcaaaa
     1141 agtgagagct tttctgccac aatggtgttt aaaacgatgt tcggtccgct tattgaaagt
     1201 gccgaaccgg gcatacggaa ctcaaacttg ttacctgtaa acgcaaacgg agaagtacgg
     1261 ttgcggtctg tggtatcctt aggaatgtac ggcagaacat ccacacccag ttttatctct
     1321 atacggtcct tcttgtcgta agatgagccg ttttgaattg cttccaggat tccggtaatc
     1381 tcatcgccta aaaataccga tatgattgcc ggaggagctt cattggcacc gagcctgtgg
     1441 tcatttccgg cactggcaac ggagaggcgt aacaaatctt gataatcatc cactgctgcg
     1501 attactgcaa ctaagaacaa caggaattgt gcgttttcca tgggagaatc gccgggctcc
     1561 aaaaggttta tacctgtgtt ggtggaaagg gaccagttgt tgtgttttcc gcttccgtta
     1621 acaccggcaa agggtttctc gtgcagtatg catgtaaggc cgtgacggga tgccactgtt
     1681 ttcattattt ccattacaat ctggttgtgg tcggtggcta tgtttgtagt ggtgaaaatc
     1741 ggagcgagct catgctgagc cggtgctacc tcattgtgct tggtctttac aaaaacgccc
     1801 aacttccaca gttcctcgtc aagttccttc ataaaagcgg aaaccctggg ctttatcgta
     1861 ctgaaataat gatcctcaag ttcctgtccc tttgggggtt tggcaccaaa aagggttctt
     1921 ccgcaataaa tcaagtcctt ccttttttca aaaaggcctt tatccaccaa gaaatattcc
     1981 tgttccggac ctactgttgt aatgatgcga gtaacattgt catgctcaaa cagcttcata
     2041 atcctgagtg cctgcttgtt cagggcatcc atggagcgaa gaagcggtgt ttttttgtcc
     2101 agtgcttcgc cgccgtagga gtaaaacacg gtgggaatgc acagggtgga atcctttata
     2161 aaggcatagg atgtaggatc ccatgcagta tagccccttg cttcaaaggt tgcacggaga
     2221 ccgccggagg ggaagctgga ggcatccggt tcgcctttga caagttcttt accggaaaac
     2281 tccaaaatga tgcttccatc ctgtaagggg gatataaagc tgtcatgctt ttcggcagta
     2341 attcccgtca tgggctggaa ccaatgggta aaatgtgtcg cacccttttc tattgcccag
     2401 tccttcatcg cacttgccac agtatcggca atgctgcggt caatgctttt gcccatttca
     2461 attgtgcgtt ttaatgcgtt ataggtatcc ttcggaagcc gttgacgcat aacacggtcg
     2521 ttgaatacca aggaaccgaa gatttcggtt atatttatgt tattgttatt cataattaga
     2581 cctccaatac atttttatta ctataattct tcattctctg tctatagtag agaaatgcct
     2641 gcatcctcac atgcggctat tgtatcctta tcccagatgg aaacctgaac ctcgccgatg
     2701 tgtgccttgc ctaaaagaag catgcacagt cttgactggc cgattccgcc tcccatggta
     2761 aggggcagtg tgccgttgag gagcatttga tggaaggaca atgcccttct gtaatcgcag
     2821 tttgccttgg tgagttgttc gtccaaggct ttggcatcaa cgcggattcc catggatgaa
     2881 acctcaaagg ccctgcccaa aagctcactg tagaacacta tatcaccgtt taaagaccag
     2941 tcgtcatagt ccggagcacg gccgtcatgc ttctgtcctg actttaatag gtcgccgatc
     3001 tgcatgatga atgcggtttt gtaccctttc aaatattcat cttcccgttg ttttggagac
     3061 agggcaggat acatgtcttc cagctcctgt gtggttacaa agcttacttc cctctccaga
     3121 gaaacctcaa tttgcggata gctttttttg agatcgtcta aagtatcgca aaccgcacct
     3181 acgatcttgc atactgtttc ctttagatag gagaggttcc ttgtctcggg ggttattatc
     3241 ttttcccagt cccattggtc aacatatacc gagtgaagat ttgtcagttc ttcatcacgg
     3301 cggatggcgt tcatatcggt gtataatcct tcaccgggtg caaaaccgta tttgtagaga
     3361 gccatccttt tccactttgc cagggaatgt accacctgtg ctacggtttg cgtttctgcg
     3421 atatcaaagc tgactggccg ttcgatgccg tttaagtcgt cattcagacc cgttgacgga
     3481 tcaacaaaaa gaggagcgga tacacgtacc aaattcaatg ctttgcaaag cctcttttgg
     3541 aaattttcct ttaggaacgc aatcgccttt tgcgtttggt acaggtctaa agcacttcgg
     3601 taaccggaag gtattactgt ctttgacata ttatcaattc ctttctgaat aaatttgatt
     3661 atttaaagct caaaggattc gaaccctttt acttttgggt cattccaccc ggtgaaatcc
     3721 agtgaataaa aaaaagacgc agcggcaata atgccagcag cgtctttgct gttgataaat
     3781 ttattataga ggtaccgatt ttatttgtca atagattttt taaagatttt ttataaaaaa
     3841 tgaattttgt agtttacaaa cttgcattct gtcatgtatt atattgataa aattatggaa
     3901 attggcttgt ttatgcaaga aaaataaaaa aacttgcaaa tttttattga cattatatat
     3961 gcggatagta tacactgtaa ttaacgaaca
//
LOCUS       DAOOJC010000221         3985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_17580_length_3985_cov_3.757252, whole genome shotgun sequence.
ACCESSION   DAOOJC010000221 DAOOJC010000000
VERSION     DAOOJC010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17580_length_3985_cov_3.757252"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLA01_06770"
     CDS             <1..302
                     /locus_tag="PLA01_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPD01039.1"
                     /translation="FHRTRERIIAHFMICYTALMIYRLLEVKLNRFDKSIHLTTRNII
                     ETLQNMQVANISDLCYSAQYTGSKTLSALEGVFALGLDKQYYLPKDLNKKCRKNL"
     gene            689..3820
                     /locus_tag="PLA01_06775"
     CDS             689..3820
                     /locus_tag="PLA01_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPD01040.1"
                     /translation="MKLIIDNRFRITVFLLTILIMSFIMMSTVKAGFSFSADPAVLNS
                     DTQINLNWSSVTDAVYYSVKRDDVLIANIDVDLEKNYLGFEDTGLSPETSYLYSVAAV
                     SSEGNIIESASRTVSTAKMKAPSITSSHLDINSKKVTLAWVNNSLAVENITVTRDDGE
                     QIAVVSSNETDISFVVPDLLPGMDTQYTLLSSDGEGHMSDESSPATITPISLPFINAS
                     IQKGTVTISWDTYPNIGEFQLERAKYMENSWGSWEIIKSKLAAGSTKTTDYVNHGTYR
                     YRLRIDNKKYTGISNISEPVTRLLAPSDLQCIPVSPGRIDLNWTNPPGSDFSLEIERR
                     DGTSSDYVTIAILDSNITSYTDTNNIELNKSYYYKVTAHDRSGSASTSAYHVNTGPPS
                     RAKSLYLEISSPTIITLNWQDTSGNESGFKVERKVDSGAFVEIASLPANTTSYTDNVN
                     NSSVYTYRVIPFNPAGNASSYSNTVSCSTSINRQPPRSLQATPVSHKQIDLSWEYADL
                     SNYKTLVERKTGENGKWSVVKVLEAGITNYSDIDLLPSTQFFYRVRTVLADNVYSIPY
                     PDNPEGMGTYTKIESPANLKAVRLSSGTIKLTWTDMVSDETGFIIERKAGNGSFIKVA
                     TQNPDSEEWYDSDILPNMSYTYRIKAINSYNSSNYSNEFYVGEFSLNSPEKLNITVTS
                     NNSITLSWENKNTNVSGFRVETKSKAGESWKETATLPSGIKNLIIKNLEENTLYRFRV
                     AAIVKTSSDTFTAYSNEIEFVMKPLSPPSNLIVKSLSPMEVLLEWKGDSENAEGYIIE
                     RGTNKMDFVEIAQVGRNTNNYTDNTVSPDNLYYYRVKLFSGSINTNYSNTGVVRTSSV
                     QVFSDLDSVPWAKTAIISLSARGIIQGRSQTPGKPAIFAPNDRITRAEFITLVVKAFG
                     IDRTPVGTFTDAKPNDWFYKSVITAKNMGIVTGTGNNYFYPNEPIKREDIAVILAKAH
                     KITGNPLPYYDDSILDKFKDKGDISSYALSSMAILNGEGIINGKSIDLLAPKDYSTRA
                     EAAVMLYNSLKKQ"
BASE COUNT         1278 a          868 c          750 g         1089 t
ORIGIN      
        1 attttcaccg cacaagagaa agaatcattg ctcacttcat gatttgctac acagctctta
       61 tgatatacag gctgcttgaa gtcaagttaa acagattcga taaaagtatc catttaacca
      121 cgagaaatat tattgaaacc ctacagaaca tgcaggtagc caacatctca gatctttgtt
      181 actctgcaca gtacacaggt tctaaaacat taagtgcatt ggagggagta tttgccttgg
      241 ggttagataa gcaatactat ctgccgaagg atctcaacaa aaagtgcaga aaaaatttat
      301 agagaccatt tccatgcaac atttttgaaa agccaaaaag aggcataaac cttgaaattt
      361 caatgtttat agcctctttt atatttctca actgtcaaac tcgggagtat actaaaaaaa
      421 tcaacaagtt tcaagagtat tttttcccaa tattaaattt atgtttcaac ataatctttt
      481 gactaaaaag ctgtccgaca tccccttctt caaacctgcc aacagcaccg ctttgacggt
      541 gcccaacgga tattataaca ggtcttttga tgcatatcaa ccttaacaat ttcctaccaa
      601 gaacccgtta attttatcat aaaattattc gataaaaaca gtgataaatc tttttgtttt
      661 ctcttattgg gtataatgga gggcagccat gaaactaatc atagataatc gttttagaat
      721 tactgttttt ttgctgacca ttttgattat gtcttttatt atgatgagta ccgttaaagc
      781 aggttttagc tttagtgccg atcctgcagt tctaaattcc gatactcaaa taaaccttaa
      841 ctggtcatcg gtaaccgatg cagtttacta cagcgtgaaa agagatgatg tccttattgc
      901 caatatagac gttgatctcg agaaaaatta cctgggtttt gaggataccg ggttatcgcc
      961 cgagacctcc tacctctaca gcgtggctgc tgtttcttcg gagggaaata tcattgagtc
     1021 tgcatcccgg acagtatcta cggcaaaaat gaaggcccct tccataacat cttcgcattt
     1081 ggatatcaac agcaaaaaag tcacccttgc atgggtaaac aactccctcg ctgtagaaaa
     1141 tattactgta acaagggatg atggagaaca gattgctgtg gtgagcagta acgaaaccga
     1201 cataagtttt gttgttccgg accttttacc gggcatggat acccaataca ccctcttatc
     1261 ctccgacggt gaaggacata tgtcggatga atcttctccc gctaccataa ctcctatttc
     1321 gctgcctttt ataaatgcat ctatacaaaa aggtactgtt actatttcat gggataccta
     1381 cccaaacata ggagagtttc agcttgagag ggcaaaatac atggaaaact cctggggttc
     1441 gtgggaaata ataaaaagca agctcgcggc aggcagcacc aaaaccacag actacgtcaa
     1501 tcatggaacc tacagataca ggttgagaat agacaataaa aagtatacgg gcatcagtaa
     1561 tatctcagag cccgttacaa ggcttctggc accttcagac cttcagtgta ttcccgtaag
     1621 tcccggcagg attgatctta actggacaaa ccctcccggc agcgacttca gcctggagat
     1681 agaaagaagg gacggaacct cctcagatta tgttaccatc gccatattag acagcaacat
     1741 tacaagctat accgatacca ataacattga actcaataaa tcctattatt acaaagtgac
     1801 agcccatgac cgaagcggca gtgcttcaac ttctgcctat catgtaaaca ccggtcctcc
     1861 ttcgcgggca aaatcgcttt atctcgaaat ctcaagtcct accataataa cattaaactg
     1921 gcaggacaca tccggtaatg aatccggatt taaggtagaa agaaaggttg actcgggagc
     1981 atttgtggaa attgcatccc tgcctgcaaa cactaccagc tatacagaca atgtgaataa
     2041 ttcatcggtt tacacctata gggttattcc ctttaatcct gcaggtaatg catcctctta
     2101 cagcaacacc gttagctgct cgacatctat taaccggcag cctccccgtt cgctccaggc
     2161 aactcccgta tcccataaac agatcgacct gtcttgggaa tatgccgacc tgtcaaacta
     2221 caagaccctt gttgaaagaa aaaccggtga aaacggaaaa tggtctgttg taaaagttct
     2281 tgaagccgga atcaccaact acagcgacat agaccttctt ccaagtactc agttctttta
     2341 cagggtcaga actgttcttg cagataatgt atattccatc ccctatccgg acaaccctga
     2401 agggatgggt acctatacaa aaattgagtc cccggctaac ttaaaagctg tccggctttc
     2461 ttccggaaca ataaaactga cctggacgga catggttagc gatgagaccg gattcataat
     2521 cgaaaggaag gccggaaacg gttcatttat aaaagttgca acccaaaacc ccgattcaga
     2581 agaatggtat gattcggata tacttccgaa tatgagctat acctatagaa ttaaggccat
     2641 taattcttat aattcttcaa attattccaa cgagttctat gtaggcgagt tttcgcttaa
     2701 ttcacctgaa aaacttaata taaccgttac atccaataat tccatcaccc tttcttggga
     2761 gaataagaac accaacgttt ccggttttcg tgttgaaaca aaatcaaaag caggtgagtc
     2821 gtggaaagaa acagctaccc tcccttcagg gattaaaaat cttattataa agaatttgga
     2881 ggaaaatacc ctgtaccgtt ttcgggtggc tgcaatagtg aaaacatcat ctgatacatt
     2941 tactgcatac agcaatgaaa ttgagtttgt aatgaagccc ctttctcccc cttcaaacct
     3001 tattgtgaaa agcctgtcac ctatggaggt tttattggag tggaagggtg actctgaaaa
     3061 cgcagaagga tacatcatcg aaagaggaac caataaaatg gatttcgttg aaattgcaca
     3121 agtgggaaga aatacaaata attacactga caatacagtt tcacccgata acttgtacta
     3181 ctacagggtt aagctgttta gcgggagcat aaatacaaac tattccaaca ccggagttgt
     3241 aagaacatca tccgttcagg tcttcagtga cctggattct gtcccttggg ccaaaactgc
     3301 tattataagc ctttcagccc gcggcattat ccagggaagg tcacaaacac ccggcaaacc
     3361 ggctatattc gctcccaatg acagaataac ccgggcagaa tttataactc ttgtggtgaa
     3421 agcattcgga attgacagga caccggtagg aacctttact gatgctaaac cgaacgactg
     3481 gttttataaa agtgttatta cagctaaaaa catgggcata gttacaggga caggaaacaa
     3541 ctatttttat cccaatgaac cgataaaaag ggaagatata gctgtaatcc ttgccaaggc
     3601 ccataaaatt accggtaatc ccctgcccta ttatgatgat tccatcctgg acaaattcaa
     3661 ggacaaggga gatatctcat cctatgccct ttccagtatg gcaatactca acggtgaagg
     3721 aatcataaac ggcaagagca ttgatctgct tgctccaaag gattattcta caagagcgga
     3781 agcggctgtt atgctgtata actctcttaa gaaacaataa tctttttcat acaacttccc
     3841 ctaaacctaa tctttatatt attataattt taccctccgt atttatagcg gattaaaata
     3901 tggagggtat aactctttgg catagttttt cgaaaatatt tatcttggta aatacagtat
     3961 attggattat aatgtgaata aagca
//
LOCUS       DAOOJC010000222         3963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_17694_length_3963_cov_3.735159, whole genome shotgun sequence.
ACCESSION   DAOOJC010000222 DAOOJC010000000
VERSION     DAOOJC010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17694_length_3963_cov_3.735159"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PLA01_06780"
     CDS             complement(<1..718)
                     /locus_tag="PLA01_06780"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPD01041.1"
                     /translation="MEIKTRLIFVRHAEAEGNLNRVFHGWTDSSITEKGHIQAKRVAE
                     RLKDVDIDVIYSSSLKRTLQTAQYIADAKNLPIIRTDKLKEINGGEWEGKRWDELPGL
                     WPDAYDSWENRPHDHKMPGGETMVDFQQRLIDEIMYIIKNNKGKNICIVTHGTALRTM
                     MCYFWGMDFTELINIKWYDNTSVSILDYEDGKFEVVLEGDVSHLEKELCTVLNQEWWN
                     EYNEKYQQRNKNQVFGNIGTN"
     gene            complement(781..1704)
                     /locus_tag="PLA01_06785"
     CDS             complement(781..1704)
                     /locus_tag="PLA01_06785"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPD01042.1"
                     /translation="MTDKSIDLSVEIAGIKFSNPVIAASGTFGFGREFSNYMDLNALG
                     GISVKGLTLEKRQGNRPPRIAETPAGILNSVGLQNPGVKAFIEEEIPFIRGFNTRIIA
                     NIAGNTIEEYCTMAEMLSDTDIDAIELNVSCPNVKKGCVAFGNSPEGIREITGRVKKY
                     CKKPLIVKLTPNVTDIKEIAIAAEGAGADAVSLINTLLGMAIDIHRKKPILANNMGGL
                     SGPAVKPIAVRMVYEVANVVKIPVIGMGGISSGEDAVEFLLAGASAVMVGTANFVNPR
                     ACVDVAEGIKQYLCKYNHGSVHEIVGKLQIY"
     gene            complement(1697..2482)
                     /locus_tag="PLA01_06790"
     CDS             complement(1697..2482)
                     /locus_tag="PLA01_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPD01043.1"
                     /translation="MSRILEEKVVSVSELAKNIFKMTVKSEYISMNAAPGQFVNVKCC
                     GGIDALLRRPISICNVDKDRATFDIVFQIKGKGTEYLSRKEAGSEVDLIGPLGNSFKI
                     SGDYKRIAVVGGGIGIFPLLYLLRELKGVDRSSYLGFRSSEYVVLLDEFGAESEALSI
                     STDDGSKGYKGLVTDLLERDIEEKGFDIIYACGPTPMLRKVCEMSKKYGIKCQVSLEQ
                     RMGCGIGACLVCACKIGKPDNWDYKHVCKDGPVFWSDEVIFDD"
     gene            complement(2602..>3963)
                     /locus_tag="PLA01_06795"
     CDS             complement(2602..>3963)
                     /locus_tag="PLA01_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPD01044.1"
                     /translation="VENIVEKEKVDGAIVQFGGQTAIKLTKALDNMGVKIFGTEPKYV
                     DAAEDREKFDEILEELGIPRPKGKTVFTLDEALTAANELGYPVLVRPSYVLGGQGMEI
                     AYNDKDITEFMEIINRVKQEHPILIDKYMMGKEIEVDAISDGEDILIPGIMEHLERAG
                     VHSGDSISVYPSQTIDEKVKHKIVDYTQKLAKALHVIGMVNIQYVYYNNELYVIEVNP
                     RSSRTVPYISKVTGIPMVNVATRIMMGRKLKDFGYGTGLYKESEYVAVKVPVFSFEKL
                     HDVDTSLGPEMKSTGEVLGIAGTFHEALYKGIIATGMKLPQKGGGILMTVRDTDKPEL
                     IQLAEEFEKLGFELYATGKTANMLNSQGIATNAVKKIGEGEPNLLDLIHSGRISLIIN
                     TPTKGRKPERDGFKIRRKAVEISIPCLTSLDTARAILECIKIGRNEKDLEVIDLSVFS
                     ELV"
BASE COUNT         1017 a         1026 c          641 g         1279 t
ORIGIN      
        1 gatttgttcc gatatttcca aacacctgat ttttatttct ctgctggtat ttctcattat
       61 attcgttcca ccattcctga tttaacacgg tgcaaagctc tttttccaaa tgagaaacat
      121 ccccttccaa cacaacttca aatttgccgt cctcgtaatc taaaatactg acagacgtgt
      181 tatcatacca ttttatattt atcaattctg taaaatccat tccccaaaaa tagcacatca
      241 ttgttcttaa cgctgttcca tgggtgacaa tgcatatatt cttaccttta ttgtttttta
      301 tgatatacat tatctcgtcg atcaaccttt gttgaaagtc caccatggtc tccccacctg
      361 gcattttatg gtcatggggc ctgttttccc aactgtcgta agcatccggc cacaaaccgg
      421 gaagctcgtc ccatcttttt ccttcccatt ctccaccgtt tatttccttg agtttatccg
      481 ttctgataat gggaaggttc ttagcatccg ctatatattg agcagtctga agggttcttt
      541 tcaaactgct tgaataaatc acatcaatat ccacatcctt aagacgttct gcaacccttt
      601 ttgcttgaat atgacccttt tcggtgatac tcgaatctgt ccaaccgtga aaaaccctgt
      661 ttaaattgcc ttcagcctct gcatgacgga caaatataag cctggtcttt atttcaatca
      721 ccctttcaaa gtatttgtat ccttcttgta ctacattata tcattaaaaa ctaaacaact
      781 ctaatatatt tgcagctttc caacaatttc atgtacactg ccatgattat atttgcaaag
      841 atattgcttt attccttctg caacgtcaac acaggccctc gggtttacaa aattagccgt
      901 tcccaccatc actgcactgg ctccggcaag caaaaattct acagcatcct ctccgctaga
      961 gattcctccc attccgatca caggaatctt gaccacattt gccacctcat agaccatcct
     1021 tactgcaatg ggcttaaccg ccggtcccga aagacctccc atattattgg cgaggatggg
     1081 cttttttctg tgtatgtcaa tagccatgcc caaaagggta ttaataaggg atacagcatc
     1141 ggcaccggca ccctccgccg ctatggctat ttctttaata tcggttacat ttggagtaag
     1201 tttaactatc aacggctttt tgcagtattt tttcactctg cccgtaatct ctcttatacc
     1261 ctccggagag tttccaaaag ctacacagcc tttttttaca ttgggacacg atacatttag
     1321 ctctatggca tcaatatccg tatccgaaag catctcggcc atggtacaat actcttctat
     1381 ggtgtttccg gctatattgg caattattct tgtattgaac ccccttataa aaggtatctc
     1441 ttcttctata aatgctttaa caccgggatt ttgaagccca acactgttta aaatacctgc
     1501 aggggtttct gcaatccttg gcggcctgtt tccctgacgt ttttccaggg taagcccttt
     1561 taccgatata cccccaaggg cattaagatc catatagtta ctgaactctc tgccaaaccc
     1621 aaaggttccg gaagctgcaa ttacaggatt ggaaaacttt attccggcta tctcaacact
     1681 caaatcaatg cttttatcag tcatcaaaaa tcacctcatc actccagaaa acaggaccgt
     1741 ccttgcaaac atgcttgtaa tcccagttat ccggcttacc tattttacaa gcacacacaa
     1801 ggcatgcccc gattccgcag cccattctct gttccaaaga aacctggcat ttgataccgt
     1861 attttttgct catttcgcac acttttctta acatcggtgt tgggccgcag gcataaataa
     1921 tatcaaatcc cttctcttcg atatcccttt cgagaaggtc ggtaaccagt cctttataac
     1981 ccttgcttcc gtcatcggtt gatatggaca atgcttcact ttcagcacca aactcatcaa
     2041 gcagcacaac atactcactg cttctaaacc ccaaatacga agacctgtca acacccttaa
     2101 gctccctcag gaggtaaagc aaaggaaata ttcctattcc tccgcccacc actgcaatac
     2161 gcttataatc tccgcttatt ttaaaggagt ttcctaaagg acctatcagg tcaacttcac
     2221 ttccggcttc cttcctggaa aggtattcgg taccttttcc tttgatctga aatactatat
     2281 caaaggttgc cctgtcctta tcaacattac aaatgctaat cggccttctt aaaagtgcat
     2341 caatcccgcc gcagcacttt acgttcacaa attggccggg tgcagcattc atagatatat
     2401 attcagattt aacggtcatt ttgaatatat tctttgcaag ctcgctgaca cttacaactt
     2461 tttcttctaa aattctggac atagtatccc ccaattattt tttatcttat tctaagactc
     2521 caacttctat aaggtggatt tcctcttata atcttcaggt ggagtagagt ctccacctga
     2581 agccccgatg tttagcttta gctaaacgag ttcactgaaa acactcaagt caataacttc
     2641 aagatctttt tcattcctgc cgatcttaat gcattctaag atagcccttg ccgtatcaag
     2701 agaagtgaga cagggtattg atatttccac tgcctttctc cttattttga acccgtctct
     2761 ttcgggcttt ctgcccttgg tcggggtatt gatgataagg cttatcctgc ctgagtgtat
     2821 aagatccagc aaattgggct caccctcacc gatcttttta acggcattgg ttgcaattcc
     2881 ctggctgttt agcatattgg cagtctttcc cgtggcatag agttcaaatc ccagcttttc
     2941 aaactcttct gccaattgta tcagttccgg cttatctgta tccctgacag tcatgagaat
     3001 acctccgcct ttttgaggaa gcttcatgcc tgtggcaata attcctttat acaatgcctc
     3061 gtggaatgtc ccggcaatac ccaacacctc accggtggac ttcatttcag gaccgagact
     3121 ggtatctaca tcgtgaagct tctcaaagga gaataccgga acctttaccg caacatattc
     3181 cgattcttta taaagcccgg taccgtatcc gaaatcctta agtttcctgc ccatcattat
     3241 ccttgtggcc acgttaacca tcggaatacc agttaccttg cttatatacg gaaccgtacg
     3301 gctggatctc ggattaacct ctattacata cagctcattg ttataataca catactgaat
     3361 atttaccatt ccgattacat gcaatgcttt agcaagtttt tgggtatagt cgacaatctt
     3421 atgcttcact ttctcgtcaa tggtctgtga aggataaacc gatatactgt ctccggagtg
     3481 aacccctgcc ctttcgaggt gctccattat accgggaata aggatgtctt caccgtcgga
     3541 aatagcatca acctcaattt cctttcccat catatattta tcaatcagta tgggatgttc
     3601 ttgttttacc ctgtttataa tttccataaa ctcagttatg tctttatcgt tataagctat
     3661 ttccattccc tgtccgccaa ggacgtatga cggtcttacc agtacaggat atcccagctc
     3721 attggcagcc gtcaaagcct catcaagggt aaacaccgtc tttccctttg gccttggaat
     3781 gcccagttct tctaatattt cgtcaaattt ctcgcggtct tccgctgcat ctacatattt
     3841 tggttctgtt ccgaaaatct taactcccat attgtccaga gcttttgtaa gctttatggc
     3901 tgtctgccct ccgaactgaa caattgctcc gtcaaccttt tctttttcaa ctatattttc
     3961 cac
//
LOCUS       DAOOJC010000223         3959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_17717_length_3959_cov_3.210809, whole genome shotgun sequence.
ACCESSION   DAOOJC010000223 DAOOJC010000000
VERSION     DAOOJC010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17717_length_3959_cov_3.210809"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..556
                     /locus_tag="PLA01_06800"
     CDS             119..556
                     /locus_tag="PLA01_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPD01045.1"
                     /translation="MKLTTKGRYGVKAMLDLALQSNGEPVSLKSIAERQYISENYLEQ
                     LFAILKKSGLVKSVRGAQGGYLLVQSPENVTIGSILRSLEGSLAPVDCVLEEDPVKCD
                     RFDSCIIKLLWEKIRDGLNSVVDSITLADLVDMYKKKNEMHIE"
     gene            607..1797
                     /gene="nifS"
                     /locus_tag="PLA01_06805"
     CDS             607..1797
                     /gene="nifS"
                     /locus_tag="PLA01_06805"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HPD01046.1"
                     /translation="MNDRHIYMDHAATTPVNPQVLEAMLPYFSEKFGNASSIYSIGRE
                     SKKALEEAREKVAKALGVMPREIFFTGSGTEADNWAIKGTAYANKDKGRHIITTSIEH
                     HAVLHACQYLESDGFTVTYLPVDENGLVSPQQVLDAITPETILISVMFANNEIGTIQP
                     IGEIGKIAREKGIIFHTDAVQAIGNIPINANDLNVDLLSLTGHKFYGPKGAGALYIRK
                     GVKIASFMQGGAQERGRRASTENVPAIVGLGKAIELATQNIDEYNKKLIELRDRTINE
                     VMEKIPFVRLNGDRYKRLPGNANFSFEFIEGESLLLMLDMKGIAASSGSACTSGSLDP
                     SHVLLAIGLPHETAHGSLRITFGEENTHEDVDYLMEVLPMIVDRLREMSPLYEKVKRG
                     NTHV"
     gene            1790..2236
                     /gene="nifU"
                     /locus_tag="PLA01_06810"
     CDS             1790..2236
                     /gene="nifU"
                     /locus_tag="PLA01_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HPD01047.1"
                     /translation="MYSEKVMDHFMNPRNVGEIENADGVGEVGNAKCGDIMKMCLKIE
                     DNVIVDAKFKTFGCGAAVATSSMATELVIGKTVEEALEITNKAVAEALDGLPPVKMHC
                     SNLAEEAIKAAIDDYMSKNGLASGDGNRNACGRRCDDIHHHDNEED"
     gene            2255..3367
                     /gene="mnmA"
                     /locus_tag="PLA01_06815"
     CDS             2255..3367
                     /gene="mnmA"
                     /locus_tag="PLA01_06815"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPD01048.1"
                     /translation="MGKKKVLVGMSGGVDSSVAAAVLLEQGYEVIGATMQIWPDMSED
                     AKLTEGGCCSLSAVDDARKIANMLGIPYYVLNFKDVFEEKVICYFKDEYLKGKTPNPC
                     IACNRYVKFESMLNKALAMGIDYVATGHYAIITYDEDKGRYLLKKSATQKKDQTYVLY
                     NLTQKQLSRILMPIGTFNKDQVREKAKELGMHVASKSDSQEICFVADNNYGKFIEENS
                     GQTISTGNFVDTEGNVLGKHRGIIHYTVGQRKGLGIAFGKPMYVVGIDAKNNNVILGE
                     EKDVFSRNLIAYDLNYISIDRLEQPMRVKAKIRYSAKEADATIYPLDKGMVKVEFDEP
                     QRAITPGQAVVFYDGDIVVGGGTIINVLQNSIKYCK"
     misc_binding    3398..3644
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3775..>3959
                     /locus_tag="PLA01_06820"
     CDS             3775..>3959
                     /locus_tag="PLA01_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPD01049.1"
                     /translation="MANVYETLKERGFIAQVTHEEQIKELLENEKVTFYIGFDATADS
                     LHVGHFLQLVVMAHMQRA"
BASE COUNT         1337 a          642 c          929 g         1051 t
ORIGIN      
        1 aatatatttc agaattattg gtttatgttt aaaaaaatat tgacaaacat ttcacgaagt
       61 gttaatctta atataataat accgagtaat atagtgggaa ttaggaggaa ctccattaat
      121 gaaactgacg accaaaggaa gatatggtgt taaagccatg cttgatcttg ctcttcaaag
      181 caatggggaa cctgtttctc ttaaaagcat tgccgagagg caatatattt cggaaaacta
      241 tttagagcag ctttttgcca tattaaagaa gtcaggttta gttaaaagtg ttaggggagc
      301 acagggtggt tacttactcg ttcaaagtcc tgaaaatgta acaatcggtt ccattctgag
      361 gtctctcgaa ggctctctgg cacctgtaga ttgtgttttg gaagaagatc ctgttaagtg
      421 tgaccggttt gattcttgta tcataaaatt gttatgggag aaaataaggg atggcttaaa
      481 cagtgttgtt gactctatta ccttggcaga tttagtggat atgtataaaa agaaaaatga
      541 aatgcatatt gagtgaaaaa atattataaa aaaactacat taataaagag taagggagta
      601 attataatga atgacaggca tatatacatg gatcatgctg ctaccacacc ggtaaaccct
      661 caagtactcg aagcaatgct tccgtacttt tcagaaaagt tcgggaatgc ttcctcaatt
      721 tattccattg gaagagaaag caagaaggct cttgaagaag caagggaaaa ggtggctaaa
      781 gccctgggtg taatgccaag ggagatattt tttacaggtt cgggtaccga agcggataac
      841 tgggcaatta aaggaactgc gtatgccaat aaagacaagg gaaggcatat aattaccaca
      901 tcaatagagc atcatgcagt attacatgcg tgtcaatatc tcgaaagtga cggctttacg
      961 gtgacatatc tacccgttga cgaaaacgga ctggtatcgc cgcagcaggt attggatgca
     1021 attacgcctg aaacaatact gataagcgta atgtttgcta acaatgaaat cggtactatt
     1081 caacctatcg gtgaaatcgg aaaaatcgca agggaaaaag gtattatatt ccatacggat
     1141 gcagttcagg ctatcggaaa catacctata aatgcaaacg atttgaacgt agatcttctt
     1201 tccttgaccg gccataagtt ttacggacca aagggagcag gggcgttata tatcagaaag
     1261 ggcgtcaaga tagcatcgtt tatgcaagga ggggctcagg aaagaggcag aagggcaagc
     1321 actgaaaatg ttcctgccat agtaggcctt ggaaaagcga ttgagcttgc aactcaaaat
     1381 atcgacgaat acaataaaaa gctcatagaa ctaagagaca gaacaataaa tgaggttatg
     1441 gaaaagatac cttttgtaag gttaaacggt gacaggtaca aaagacttcc gggtaatgca
     1501 aatttttcct ttgagtttat tgaaggggag tcgcttttat taatgcttga tatgaaagga
     1561 attgctgctt caagcggttc tgcatgcaca tcaggttccc ttgacccgtc ccacgtactt
     1621 ttagcaatcg gtttgcccca tgaaacagca catgggtcat tacgaattac ttttggtgaa
     1681 gaaaacactc atgaagacgt tgattacttg atggaagtac tgcctatgat agttgacagg
     1741 ctgagggaaa tgtcgccatt atatgaaaaa gtgaaaagag ggaatacaca tgtatagtga
     1801 aaaggttatg gatcatttta tgaacccaag aaatgtaggg gaaattgaaa atgctgacgg
     1861 cgttggagag gttggcaacg caaaatgcgg agatattatg aagatgtgtc tcaaaattga
     1921 agataatgta attgtagacg ccaaatttaa gacctttgga tgtggtgcag cggttgcaac
     1981 cagcagcatg gcaactgagc ttgtaattgg aaaaactgtt gaagaggctc ttgagataac
     2041 caacaaggct gttgctgagg ctttagacgg tctcccacct gtaaaaatgc actgttccaa
     2101 tcttgcagaa gaggcaataa aagcagctat tgatgattat atgagtaaaa acggtcttgc
     2161 aagcggtgat ggaaaccgca atgcttgcgg aagaagatgt gatgacatcc atcatcatga
     2221 taacgaagaa gattaaagga cggtatttct tacaatgggc aagaaaaagg ttttagtcgg
     2281 tatgagcggc ggcgttgaca gttctgtagc tgccgctgta cttttagaac agggttatga
     2341 agtaatcggg gcaaccatgc aaatatggcc cgatatgagc gaagatgcca agcttactga
     2401 aggcggctgc tgctcgcttt cagctgtcga tgatgcacga aaaattgcaa acatgctggg
     2461 gattccgtat tatgttttaa acttcaagga tgtgttcgaa gaaaaagtaa tatgctactt
     2521 taaggatgaa tatctcaagg gaaagacgcc aaatccctgc attgcatgca acagatatgt
     2581 caaatttgaa tccatgttga ataaggcttt agccatggga atagactatg ttgccacagg
     2641 tcattatgca attattacct atgacgaaga caagggaagg tatttgctta agaaatcggc
     2701 tactcaaaaa aaagaccaga cctatgttct ttataacctt actcagaaac aactcagccg
     2761 aatacttatg cccataggca cgttcaataa ggatcaggtc agggagaaag caaaggaatt
     2821 gggtatgcat gttgcttcaa aatccgacag ccaggaaata tgctttgtag ccgataacaa
     2881 ctacggtaaa ttcattgaag aaaactcggg ccaaacaata agtaccggaa actttgtaga
     2941 tacagaaggc aatgtattgg gaaaacacag gggaataatt cattataccg tgggtcagag
     3001 aaaaggtctc ggtattgcat ttggaaaacc catgtatgtt gtcggtatcg atgcaaaaaa
     3061 taacaatgtt atactgggtg aggaaaaaga tgtgttctcg agaaatctta tagcctatga
     3121 cctaaactat atatcgatag acaggcttga gcagcccatg agagttaagg caaaaataag
     3181 gtattcagct aaagaagcgg atgcaactat atatcctctt gataaaggca tggtaaaggt
     3241 agagtttgat gagccgcaga gggccataac tcccggacag gctgtggtgt tttatgacgg
     3301 cgatatagta gtgggcgggg gaacaatcat caatgtacta caaaattcaa ttaagtattg
     3361 taagtaagat gattatatgt tacaatacta aataatgcaa aaaacgatga cagagaagat
     3421 gtatatctta cagtagccat tcaaagatga catttagaga gaagtccggc cggtgaaagg
     3481 acttttgcac ttgaatgagc gaattaccct cttgagtttc gcactgaaat ctaagtaggt
     3541 gttgacggta gccagccgtt acagtggcaa ttttaataag gcgtgtcagc aaatattctg
     3601 acatgcgaat taaggtggta ccacgggagc ttctcgtcct tagatcagga tgacaggctt
     3661 tttttattgt aaactttgat aagggtataa taaagaaaaa tgcggaaggc tgcaaaactt
     3721 aagtaactgg tatattattt cattatatta gaatattaat gcggaggtgt ttttatggca
     3781 aatgtatacg aaaccctaaa agaaagaggg tttattgccc aggttactca tgaagaacaa
     3841 ataaaagaat tattggaaaa tgaaaaagtt actttctata ttggatttga cgcaactgct
     3901 gacagccttc atgtaggaca ttttctacaa ttggttgtta tggcacatat gcaaagggc
//
LOCUS       DAOOJC010000224         3958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_17721_length_3958_cov_3.772226, whole genome shotgun sequence.
ACCESSION   DAOOJC010000224 DAOOJC010000000
VERSION     DAOOJC010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17721_length_3958_cov_3.772226"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLA01_06825"
     CDS             complement(<1..258)
                     /locus_tag="PLA01_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD01050.1"
                     /translation="MRKTKSELPSDHKIIVVDDERGILDSLSVIINRLGYRYTGVNNP
                     LEAIETIKREDFDLLVLDFLMEPLQGDEVVREIRKFNKDIYI"
     gene            complement(504..878)
                     /locus_tag="PLA01_06830"
     CDS             complement(504..878)
                     /locus_tag="PLA01_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD01051.1"
                     /translation="MEKTKILVVDDAVFMRKYLGNTLNSLGYCNVEFAENGYEAVEKA
                     RKMQPQVVTLDVSMPGMDGIETIEQILKVSSGSKIIMVSAVTSDKIIRQAINNGAVDY
                     IPKPFSRKDVEKSLKKYVKTVV"
     gene            complement(1221..2720)
                     /gene="thrC"
                     /locus_tag="PLA01_06835"
     CDS             complement(1221..2720)
                     /gene="thrC"
                     /locus_tag="PLA01_06835"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPD01052.1"
                     /translation="MHYESTRGGLKSVLSAEAIKQGIAPDGGLFVPEKRVKLSMDEIA
                     SLVGMNYRERAVHILKPYLTDYSDEEIKDCVYNAYTEEKFGHPGIAPLFKLNENVNIL
                     ELWHGPTCAFKDMALQILPHFLVKAVRKTGETGEIVILVATSGDTGKAALEGFRDVEG
                     TRIIVFFPEKGVSLVQKMQMVTQEGKNVYSIGVDGNFDDTQNGVKAIFTDDELKNKME
                     ENGLKFSSANSINWGRLVPQIVYYFSAYADMVNSGEIQTGEKINIVVPTGNFGNILAS
                     YYAMEMGLPVNRLICASNDNNVLSDFINTGTYDKNREFKRTISPSMDILISSNLERLL
                     FELTDHDSALINKWMTQLKNDGMYSVDPDTRKRICGIFWSGFSNEVETLKTIEAIYKE
                     YSYVIDTHTAVGVDVYDKYVISTGDMTKTVIVSTASPFKFNASVVKAVLGEEALKGRT
                     EFELLEILSKECGLKIPEGLKNLDKRAVKHKTVCGSNHMKQQVEKILGI"
     gene            complement(2860..3957)
                     /locus_tag="PLA01_06840"
     CDS             complement(2860..3957)
                     /locus_tag="PLA01_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPD01053.1"
                     /translation="MGGGGIKGIAYIGMYEAAEAKGLRFDTIAGVSAGALVGALIGAG
                     YKASELKRILYETEFDKINVRDISREMPVVQRFIQYYENGRYNGDKSIEDFLGIPVIT
                     KNGYNTIEPFEFSGRRGNIFKNIITYSKEKCLFDGDFIEEWMYKTLLKKGVKTFADIR
                     TKKTDESNPKGYKVRMTAVDVNRGKVVILPDDISFYGIDPDRLEVAKAIRMSTSVPFA
                     FKPVEINKMYGNSSKKYYLVDGGVLDNFPLWLIKPSLSIPLIGCILKGRDKFNILDSL
                     SILKKIISIVHDYGIPDVKDYKNCFIIKIDTGDISFLDFNLDDRKKEYLIQSGRKALY
                     YALVRKYPMSIMSIIRRCGLIYILRRLLGSC"
BASE COUNT         1205 a          877 c          578 g         1298 t
ORIGIN      
        1 aatgtatatg tccttattga attttcttat ctctcttacc acttcatcgc cctgtaacgg
       61 ttccattaga aaatccagta ccagaaggtc aaaatcctct cttttgattg tctcaattgc
      121 ttcaagaggg ttatttactc ccgtataccg gtaaccaagg cgatttatta tgactgataa
      181 ggaatccaat attcctcttt catcatcaac aacaattatt ttatgatccg aaggaagttc
      241 cgatttggtt tttctcacag catatcctcc cttatagcag aaacctcgtc tgaaactcca
      301 aaggagcctt tttaactcaa aggacctgca tataatggta aattatgtag ccgaattata
      361 gataactttt aattaatata tcgacttaat tatttaataa tataacacat aactgcatta
      421 tatttcacca aaacagacta taaagatgtt aatagatttt gacaatttta gatcaattta
      481 gtgagatgaa gggataaagt cttctacact accgttttga catacttttt aagacttttt
      541 tctacatcct ttcggctaaa gggcttcggg atatagtcta ctgcaccgtt atttattgcc
      601 tgccggataa ttttatcgga agtaactgcc gagaccataa taattttgct gccggaactg
      661 accttaagaa tctgttctat agtctcaata ccgtccatac cgggcattga tacatcaagg
      721 gtcaccactt gaggctgcat ttttctggct ttctcaacag cttcataacc gttctcggca
      781 aattccacat tgcaataacc taaggaattt aaggtattgc ctaaatattt cctcataaaa
      841 acagcatcat ccaccaccag aatctttgtt ttttccatcc cgcctaccac ctctattccc
      901 ggttttaaaa aaataaatct aatatataag tcggtaaaaa atgagactta tttatacttt
      961 ttaatactat attctgaaac caaaaagcta aaggggataa agccctttcg cttttcggtc
     1021 atatcacttc gtgaaatccc actaatgaaa agtactgtct ttgtattaca aaagcaatgc
     1081 cccgatgact taaataagcc atgaaatgtt ataggtaata cataaaaggc ttttagtaac
     1141 ctttattaaa caacatttga atgcgggtag cggaaaaccc cacagatact cataactcag
     1201 ctgtcgatac aaaaatatgt ttagattcct aaaatttttt caacctgctg cttcatatga
     1261 ttgctgccac aaacggtttt atgcttcaca gcccttttat caaggttctt taagccctca
     1321 ggtatcttaa gtccacactc tttagataaa atctcaagaa gctcaaattc tgttctgccc
     1381 tttaaggcct cttctcctaa aaccgccttt acaacactgg cattaaattt aaacgggctt
     1441 gcagttgata ctattactgt cttggtcatg tcacccgtgg atattacata tttgtcatac
     1501 acgtctactc caacagcagt gtgtgtatca attacatagc tatattcctt ataaattgct
     1561 tcaatggttt ttaatgtctc aacctcattt gaaaaaccgg accagaaaat gccacatatc
     1621 ctctttctag tgtcgggatc gacactgtac atgccatcat tcttcaattg agtcatccac
     1681 ttattgataa gtgcggaatc atgatctgta agctcaaata aaagcctctc aagattactc
     1741 gaaatcaata tatccatgga tggagatatc gttcttttaa actctctgtt tttatcatat
     1801 gttcctgtat taataaaatc cgaaagaaca ttgttgtcgt ttgatgcaca aataagccta
     1861 ttgacgggaa gccccatctc catggcatag taagatgcaa gtatatttcc aaaattacct
     1921 gtcggaacta caatatttat cttttcacct gtttgtatct caccgctatt aaccatgtcc
     1981 gcatatgcag aaaagtaata cactatctga ggaacaagcc ttccccagtt aatggaattg
     2041 gccgatgaga acttcaaacc gttttcctcc atcttgtttt tcagttcatc atctgtaaat
     2101 attgccttga ctccgttttg agtatcatca aaatttccgt ccacaccaat ggaataaacg
     2161 tttttccctt cctgcgtaac catctgcatt ttttgaacta ggctgacacc cttttcaggg
     2221 aaaaacacaa ttattctcgt accttcaaca tccctgaaac cttccaatgc agcctttcct
     2281 gtatctccgg aggtagcaac cagtattaca atttcgcctg tctctccggt ctttctgacc
     2341 gccttaacaa gaaaatgagg taaaatctga agagccatat ccttaaatgc acatgttgga
     2401 ccgtgccaca actcaagaat attaacattt tcattcagtt tgaacaatgg tgctattccc
     2461 ggatgtccaa atttttcctc agtataagca ttataaacac agtcttttat ctcttcatca
     2521 gaataatcgg taaggtatgg ttttaatatg tgtaccgctc tttcccggta gttcataccg
     2581 actaaggacg ctatttcatc cattgaaagc ttaacccttt tctcgggaac aaaaagacct
     2641 ccatccggtg ctataccttg ctttatagcc tcagcagata aaaccgactt taatccgcct
     2701 cgtgtactct catagtgcat tttgcttcct cctatatata caaatcaaaa aacaatcagt
     2761 ccaaatcaat tagtcttaat atcctacttc tatataatat ataatatatt ttaaaaaaaa
     2821 taaagaatat tttctgtaat tcttataaat gtgcaaacat caacacgaac ccaataaccg
     2881 ccttaaaatg tatataagac cacatcttct aattatactc attatactca tggggtattt
     2941 cctcacgaga gcataatata aggcctttct ccctgattgt ataagatatt ctttctttct
     3001 gtcgtctaaa ttgaaatcca aaaaagaaat gtctcctgta tcaattttta ttataaagca
     3061 gttcttataa tcctttacat caggaatgcc ataatcatgt acaatagaaa taatcttttt
     3121 taatatgctt aacgaatcta atatgttaaa cttatctctg cctttgagaa tacagccaat
     3181 taacgggata gataaggagg gctttatcag ccaaagagga aaattatcca gaactcctcc
     3241 atccaccaga taatattttt ttgatgaatt gccgtacatc ttgtttattt caacaggttt
     3301 aaaagcaaaa ggcacgctgg tactcattct aattgccttc gccacttcga ggcgatcagg
     3361 atcaataccg taaaacgaaa tatcgtcggg aagaattaca actttgccac ggttgacatc
     3421 cacagcggtc attctaactt tataaccctt tggattggat tcatcggttt tttttgtcct
     3481 gatatccgca aaggttttta cacctttttt aagcagggtt ttatacatcc attcctcaat
     3541 aaaatctccg tcaaaaagac atttttcttt gctgtaggtg attatattct tgaatatatt
     3601 tccccgccga cctgaaaatt caaagggttc aattgtgttg taaccatttt ttgttatgac
     3661 aggtattcct aagaaatcct caatgctttt atcaccgtta tatctaccgt tctcgtaata
     3721 ctggataaat ctttgcacta ccggcatttc ccgggaaata tccctcacat taatcttgtc
     3781 aaattcagtt tcataaagga ttcgtttgag ttccgaggct ttgtaacctg caccgattaa
     3841 agccccaacc agagctcctg cagatactcc tgcaatggta tcgaatctaa gcccctttgc
     3901 ctcagcagct tcatacatgc ctatataagc aatacccttt attccaccgc ctcccaac
//
LOCUS       DAOOJC010000225         3943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_17798_length_3943_cov_3.516718, whole genome shotgun sequence.
ACCESSION   DAOOJC010000225 DAOOJC010000000
VERSION     DAOOJC010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17798_length_3943_cov_3.516718"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2138
                     /gene="leuS"
                     /locus_tag="PLA01_06845"
     CDS             <1..2138
                     /gene="leuS"
                     /locus_tag="PLA01_06845"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPD01054.1"
                     /translation="ISYDWDREVATCHPDYYKWTQWMFLQLYKRGLAYKKKSYVNWCP
                     SCATVLANEQVVNGACERCKATVAKKELEQWFFKITDYAQRLLDDIDKIKGWPDKVKT
                     MQQNWIGRSEGVEVDFKVDGMDKVVRIYTTRPDTIYGVTYMVIAPEHPIVKELIKGTE
                     QEEACKEFINKMSYLNEIDRTSNEVEKEGVFTGRYVINPLNNDKVPLYLANYVLAEYG
                     TGVVMAVPAHDQRDFEFSKKYGLPIKVVIQPEGQQLDASKMTGAFEDIGYLTNSGKFD
                     GVRSDEAIGKIIEYIEQMGYGEKKVNFRLRDWLISRQRYWGAPIPVIYCDDCGIVPVP
                     EDQLPVILPTDVKFTGQGESPLSTSESFITAKCPKCGKMGRREIDTMDTFVCSSWYYL
                     RYCDPCNAKEPFDKESIEYWMPVDQYIGGVEHAILHLLYSRFFMKALYDMGYVAYDEP
                     FTNLLTQGMVLKDGAKMSKSLGNVVSPEEIIEKYGADTARLFILFASPPEKDLEWSDQ
                     GVEGCYRFINRVWRIINDFIDTVKKDQTIDTSKFTGEDKELWFMVNNTIKRVTDDIGQ
                     RFNFNTAISAIMELVNSLYYYKDKVADENRNSALLKAAIENMIIMLAPFIPHAAEELW
                     EMVGKEGSVHDQSWPKYDPDALIKDEIEIVLQINGKVRDKLVIPSNLTREQVEERALK
                     NEKIMAETQGKNVVKVITVPGKLVNIVVK"
     gene            complement(2323..>3943)
                     /locus_tag="PLA01_06850"
     CDS             complement(2323..>3943)
                     /locus_tag="PLA01_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01055.1"
                     /translation="VNENGTNPVESYLANNRIETTIEIPLPPTIQAFDLDYNVLSRKI
                     TYPLYNEPITATLPSGYSWTGNAEGSLDVVNQSENLLRDFKVTNNPSINEPSTTITRE
                     PQIHATLKRPDFGDDPQNRKWSDWNDPYVPVSREGKTTFDGEVYRPYEYYCGFEDCQD
                     CPHDNTAMAEFEPGSNITKARAFIYNGKATIEPKAYSNRIDYSDSAKEINLLWASNPY
                     KFNVVRWMYHMDENGKLINPTQVDGKYQRTFTQQNSGKVNWSIPASMGANYKRSRDAA
                     RKGDTRNSELDRAVFASDKVYQNLAYPIRSGYYFNPTGTYQFTVETVTYKPTTADTDE
                     HKNLVQALINSFRYESNLVYVNNKNQAVDIQDQPATKKSTVYTAKYAVITASDPIGLN
                     GVNWLQIIDRKADPSRYVKTFEEIKHSTEVDGSNTHVFWKNVLEGYKESGTILSYNNF
                     KYREYVKPGQTMYKITEKTTVTIIVNPQNVKAYTHIQMADGKYSVRAYFDETALTNIS
                     SALPTIKGISLDSIEITVVGSRYDDMGYDEH"
BASE COUNT         1118 a          686 c          929 g         1210 t
ORIGIN      
        1 gtattagcta tgactgggac agagaggttg ctacctgcca tcctgattac tataaatgga
       61 cacagtggat gtttttacag ctctataaaa gaggccttgc gtataaaaag aaatcctatg
      121 taaattggtg tccttcctgt gcaactgttt tagctaacga gcaagtagtg aatggtgcat
      181 gtgagcgttg taaagctact gtagcaaaaa aggaattgga gcagtggttc tttaagataa
      241 ctgactatgc ccagaggctt ttagacgata ttgacaagat taagggatgg ccagataagg
      301 ttaaaacgat gcagcagaac tggattggca gaagtgaagg ggttgaggtt gactttaagg
      361 ttgacggtat ggataaggtt gtgaggattt acaccacaag accggatacc atatatggcg
      421 ttacttacat ggttatagct ccggagcatc ccatagttaa ggaattgatt aagggaacgg
      481 aacaggaaga ggcttgtaag gaatttataa ataagatgtc ctatctcaat gaaattgaca
      541 gaacatccaa cgaggtggaa aaggaaggag tatttacagg aagatatgtt ataaatccct
      601 tgaacaatga taaggtacct ctttatcttg caaattatgt tcttgcagag tacggtaccg
      661 gggttgtaat ggctgttccg gcacatgacc agagggactt tgagttttcg aagaagtatg
      721 gtttgccgat taaagttgta attcagccgg aaggtcaaca gcttgatgct tcaaagatga
      781 caggagcctt tgaggatatc ggatacctta caaattcagg taagttcgat ggtgtcagaa
      841 gcgacgaggc tataggtaaa ataattgaat acattgaaca gatgggttac ggtgagaaaa
      901 aggtgaactt caggctaaga gactggttga tttcaagaca gaggtactgg ggggcaccga
      961 tacctgttat atattgtgat gactgcggta tagtgcctgt accggaagat cagcttcccg
     1021 ttatattacc gacggacgtt aaatttacag gtcagggaga gtctcccctt tccacaagtg
     1081 aatcttttat tactgcaaaa tgtcctaaat gcgggaagat gggcagacgt gaaatagata
     1141 caatggacac ttttgtgtgt tcttcatggt attacttgag gtattgcgac ccgtgcaatg
     1201 ctaaagaacc ttttgataag gaaagcatag aatactggat gcccgtggac cagtatatcg
     1261 gcggtgtgga gcatgcaatt ctgcatcttc tgtattcaag attttttatg aaggcccttt
     1321 atgacatggg atatgttgcg tatgatgaac cctttacaaa tcttctgact cagggtatgg
     1381 tgcttaagga cggggctaag atgtccaagt ctttaggaaa tgttgtgagc cctgaagaaa
     1441 taattgaaaa atacggtgct gacactgcaa ggctgtttat tctgtttgct tctccgccgg
     1501 aaaaggatct ggaatggagc gatcagggtg ttgagggttg ttataggttt atcaacaggg
     1561 tctggagaat tattaatgat tttattgata cggtaaagaa agaccaaacc atcgatacct
     1621 ccaaatttac cggtgaggat aaggaactgt ggtttatggt taataacacc attaagagag
     1681 taacggatga tatcggacag aggtttaact ttaacactgc gataagtgca attatggaat
     1741 tggttaactc attatattat tacaaagaca aagtggcgga tgaaaatagg aacagtgcat
     1801 tgttaaaagc agcaatagag aatatgataa ttatgctggc accctttatt cctcatgcag
     1861 cggaagagtt atgggaaatg gtgggcaagg aaggaagtgt tcatgatcag agttggccga
     1921 agtatgatcc cgatgcactt ataaaagacg aaatcgaaat tgtgctccag atcaacggaa
     1981 aggtaaggga taagcttgtt attccgtcaa acctgaccag agaacaggta gaggagaggg
     2041 cattaaagaa tgaaaagata atggccgaaa ctcagggtaa aaatgtggtg aaggttatca
     2101 cagtaccagg caagcttgtt aatatcgtag tgaagtagaa ataaacattt ggtatttgtt
     2161 gctcagttca aaattgcctg tacctatggc tgttacaata gccggggtac aggctttgtg
     2221 tttttgtgag ggattctgtt tgctagtggg atttcacgaa gtgaaatggc caaaaagcaa
     2281 gggggcttga tgcccccttg ctcttgctaa attcacatgt agttaatgtt catcataacc
     2341 catgtcatca tatcttgaac caacaaccgt tatttctata ctgtccagag aaatgccttt
     2401 tatagttgga agtgcactgc tgatgttagt aagtgcagtt tcatcgaaat aggcacgtac
     2461 actgtatttg ccatccgcca tctgtatatg ggtgtacgct tttacgtttt gtggatttac
     2521 aatgattgtt accgttgtct tttcggttat tttatacatg gtctgacccg gtttaacgta
     2581 ctctctgtat ttaaaattat tatagctcag aattgtaccc gattccttat aaccctccaa
     2641 gacgtttttc cagaatacat gcgtattcga accgtccact tccgttgagt gcttgatttc
     2701 ttcaaatgtt tttacatagc gggacggatc ggcctttctg tctattattt gaagccagtt
     2761 aacaccattc aagcctatcg ggtcagatgc tgtaattaca gcatattttg cagtataaac
     2821 agtacttttt ttggttgcag gctggtcctg tatatccact gcctgatttt tattgtttac
     2881 atacacaaga tttgattcgt atctaaagga gttgataagt gcctgtacaa ggttcttgtg
     2941 ctcatcagta tcagcagtgg tcggtttata ggttacagtc tctacggtga attgataggt
     3001 accggtgggg ttaaagtagt agcctgacct gatgggatag gctaaattct gatatacttt
     3061 atccgatgca aaaacagccc tgtccagttc ggaatttctt gtatcgcctt ttcttgcagc
     3121 atccctgctt cgtttataat tggcacccat gctcgccggt attgaccagt tgacttttcc
     3181 gctgttttgc tgtgtaaaag ttctttggta tttaccgtct acctgtgttg gattaatcag
     3241 tttaccgttc tcgtccatat ggtacatcca gcgaacaacg ttaaatttat agggattgct
     3301 tgcccaaagt agattaattt cctttgcaga gtcagaataa tctatcctgt tactgtaggc
     3361 cttaggttct atggtagcct ttccattata gatgaaagcc cttgccttgg ttatgttgct
     3421 tccaggctcg aattcagcca ttgcagtatt atcatgaggg cagtcttggc aatcttcaaa
     3481 accgcaataa tattcatatg gccgatagac ctcaccatca aaggttgtct taccttctct
     3541 tgacactgga acataggggt cattccagtc ggaccatttt ctattttggg ggtcatcacc
     3601 aaagtcaggt ctttttagcg ttgcatgtat ttgaggttct cgagttattg ttgtagatgg
     3661 ttcgtttatt gagggattat ttgttacttt aaaatccctc aacaagtttt cagattgatt
     3721 tactacgtca agagaacctt ctgcatttcc agtccatgaa taacccgacg gaagagtagc
     3781 tgtaataggc tcattataga gtgggtaagt aattttcctg gataatacat tataatccaa
     3841 atcaaacgcc tgtattgtcg gaggcagagg aatttcaatg gtggtttcta ttctattgtt
     3901 ggcaagatag ctttctacag gattcgttcc gttttcatta aca
//
LOCUS       DAOOJC010000226         3940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_17812_length_3940_cov_3.178893, whole genome shotgun sequence.
ACCESSION   DAOOJC010000226 DAOOJC010000000
VERSION     DAOOJC010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17812_length_3940_cov_3.178893"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..225
                     /locus_tag="PLA01_06855"
                     /pseudo
     CDS             7..225
                     /locus_tag="PLA01_06855"
                     /inference="COORDINATES: protein motif:HMM:NF036812.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
     gene            250..903
                     /gene="thiE"
                     /locus_tag="PLA01_06860"
     CDS             250..903
                     /gene="thiE"
                     /locus_tag="PLA01_06860"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HPD01056.1"
                     /translation="MTKREKLKKLDAGLYCITSEEHSKGRSNIEVVESMIKAGVGIIQ
                     YREKDKKLSEKYYECLKIREMTEKAGIIFIVNDSVDIAMLVKADGVHIGQDDIPIEKV
                     RELVGEEMIIGLSTHSPEQAEDAVRRGADYIGVGPLYRTYTKKDVCEPVGLGYLDYVV
                     KNIKIPFVAIGGIKEHNMDEVLSHGAGCIAMVTEIVGADDIEEKIARLKSKFGEGFN"
     gene            905..2203
                     /gene="thiC"
                     /locus_tag="PLA01_06865"
     CDS             905..2203
                     /gene="thiC"
                     /locus_tag="PLA01_06865"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HPD01057.1"
                     /translation="MYKTQMDAAKKGIITNEMKTVAKKEGFLAEELRELVACGRVIIP
                     ANKNHKGIDPEGIGEGLRTKINVNLGISKDCCNFDIELEKARKAIELKAEAIMDLSSY
                     GKTREFRKQLINISPAMIGTVPVYDAVGFYDKELKGITAEEFFEVVERHAEDGVDFMT
                     VHAGINRETARRFKENGRLTNIVSRGGSLIFAWMELTGNENPFYEQFDRLLRIFEKYD
                     VTVSLGDALRPGSINDSTDAPQIQELIVLGELTKKAWDRNVQVMIEGPGHMAINEISA
                     NMVLEKRLCHGAPFYVLGPIVTDIAPGYDHITGAIGGAIAAANGADFLCYVTPAEHLR
                     LPDIDDMKEGIIASKIAAHAADIAKGISGAREWDYRMSEARRNLDWNRMFELAIDGEK
                     ARKYRESSKPEHEDTCTMCGKMCAVRNTNRVLNGEDINVL"
     gene            complement(2524..2709)
                     /locus_tag="PLA01_06870"
     CDS             complement(2524..2709)
                     /locus_tag="PLA01_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HPD01058.1"
                     /translation="MSNSKNSKPSLDRLKYEIASQAGINLKQGYNGDLKASEAGKIGG
                     NITKKLIEMAEKQLSKK"
     gene            2967..3491
                     /locus_tag="PLA01_06875"
     CDS             2967..3491
                     /locus_tag="PLA01_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPD01059.1"
                     /translation="MYSLNIKSNILYLRDIGMDDLGHILKLFNDIDSFKFATGIYEPV
                     TLNEIKRFFLRNLFSQKVFCSGIFSIGSGKIIGMLKGQLAYNYEDTAWINTMIIEPEV
                     QRKGYGTEAVNLLIGYLKENSKVKKLYLSVLEENYMGKHFWEKQNFSEIMRIDKNSAT
                     GCKHRGISIMYRQI"
BASE COUNT         1302 a          613 c          966 g         1059 t
ORIGIN      
        1 gatatgatgc cgaagctttt agcgggcagg gacagtgcga aagatgtggg tgtaagggtt
       61 tcaatggagc ttaaaattga tagaaaagca tcggtggaag atctttcggc tgcaaatttc
      121 aaaagaatcc aggaagcgtt gagggtgata gaggaaaacc tgaaggtact gggcaggtat
      181 gaactttcaa agcaatatga gaattacagg tttattgcat acggtattga aaaggagtat
      241 ttcaaggtca tgaccaagag ggaaaaacta aaaaaactgg atgcagggct gtattgcatt
      301 acttcagagg agcattcaaa gggccgcagc aatattgaag ttgtggaaag tatgataaag
      361 gccggagttg ggataattca gtacagggag aaggataaaa agctgtcgga aaagtattat
      421 gagtgcctga aaattcggga aatgaccgaa aaagccggta ttatcttcat agtaaatgac
      481 agtgtggaca tcgcaatgct ggtcaaagcc gatggggtgc atattggaca ggatgacata
      541 cccattgaaa aggtaaggga actggtgggg gaggaaatga ttatcggact ttccacccat
      601 tcgccggagc aggccgagga tgcagtaaga cggggggccg actatatcgg ggtagggccc
      661 ttgtacagga catataccaa aaaggatgta tgcgaaccgg tggggcttgg gtacctggac
      721 tatgtggtga aaaacataaa aatacccttt gttgccattg ggggtatcaa ggagcataac
      781 atggatgagg tcttaagcca tggtgccggg tgcattgcaa tggttacgga gattgttggg
      841 gccgatgaca tagaggagaa gatcgcaagg ttaaaatcaa agtttggaga ggggtttaat
      901 taaaatgtat aaaactcaaa tggatgctgc aaaaaaggga attataacaa atgaaatgaa
      961 gacggtggca aaaaaggagg gctttttggc tgaagaatta agagagcttg ttgcctgcgg
     1021 aagggtgata atacctgcta ataaaaacca caaaggcatt gatcctgaag ggataggcga
     1081 aggcctaagg acaaagataa acgtcaatct gggaatatct aaggactgct gtaattttga
     1141 cattgaactg gaaaaggcca gaaaagccat cgagcttaag gcagaagcga taatggattt
     1201 gagttcctac ggaaaaaccc gggaattccg aaagcagctg ataaacattt cacctgcaat
     1261 gataggtacc gtacctgtat atgatgccgt aggtttttat gacaaggagc taaagggcat
     1321 aactgccgaa gagttttttg aggtagtgga aaggcatgcc gaagacgggg tcgactttat
     1381 gaccgtacat gccggtataa accgggagac agcccggcgt ttcaaggaga acgggagact
     1441 taccaatata gtgtccaggg gcggttcatt aatatttgca tggatggagc tgacgggaaa
     1501 tgaaaatccg ttttatgagc agtttgacag gctccttcgg atatttgaaa aatacgatgt
     1561 gaccgtcagc ctcggtgatg cattaagacc cgggagcata aacgattcta ccgatgcacc
     1621 gcagattcag gagcttatcg tgctggggga actgacaaaa aaggcatggg atagaaatgt
     1681 gcaggttatg attgagggac cggggcatat ggctataaac gaaatttctg ccaatatggt
     1741 tctggagaaa aggctttgcc acggtgcacc cttctatgtc ttaggaccga ttgtaaccga
     1801 tatagcaccg ggatatgacc acattacagg tgccataggc ggggcaattg ctgccgctaa
     1861 cggtgccgac tttttgtgtt atgtgactcc tgcggagcac ttaagacttc ccgatatcga
     1921 tgacatgaaa gaaggaatca tagcatcaaa aatagctgcc catgcagcgg atatagcgaa
     1981 aggaatcagc ggtgcaagag aatgggatta cagaatgagt gaagcaagaa gaaacctgga
     2041 ctggaatagg atgtttgaac ttgccataga cggtgagaag gcgagaaagt atagggaaag
     2101 ctcaaaaccg gagcatgaag atacctgtac catgtgtgga aagatgtgtg cggtcagaaa
     2161 tacaaaccgg gtattaaatg gcgaggatat aaacgtttta tagggttata tggatcagtt
     2221 cgatgaaaaa aagagaataa aagaaaaagg gggcaagggg gaatcacccc cttgttataa
     2281 gaaaatcata atactaaata ttaaaggagc ttgtttgctc aaaaggttta gacaaaaaac
     2341 tgcagcttat tgtttcctgc tgcagagcat acaataatgt tgtatagagg aaaaaatata
     2401 tttaagtcct ttcgaacaat tatagtatac aacatatact taaataagtt aatcggtaaa
     2461 ctgtacaata attaaatcaa atagttgttg cttttttgtg aaaattaagc aaattccata
     2521 atactatttt tttgacaatt gcttttcagc catttcaata agcttctttg taatgtttcc
     2581 gcctattttt cctgcttccg aagctttgag atcaccgttg taaccttgct ttaaatttat
     2641 tcctgcctgc gatgctattt catatttcaa tctgtcaagg gatggttttg aatttttgct
     2701 gtttgacata gtattcacct ccttgcccac ggttgaagta aactgggcat atatattttt
     2761 tgagataaca actgttttaa aacatgaaaa ttgttgaagc aaaaataaag gaatttcagg
     2821 aagttatgga gaaattatta atatatctta ttcatcacaa agccgggtgc attgattaac
     2881 tcaggaaaaa tgttctgata gctgataacc ggccgtattt tcactgacaa gaataacatg
     2941 caccttcaaa tacttagggg gatcctatgt attctttaaa tattaaaagc aacatattgt
     3001 acttaaggga tattggtatg gacgacctgg gccatatatt aaaattgttc aatgatattg
     3061 atagtttcaa atttgcaacg ggtatttatg agcctgtgac attaaatgaa ataaaaagat
     3121 ttttcttgag aaatcttttt tcacagaaag ttttctgttc cggcatattc agtattggct
     3181 ccggtaaaat aatagggatg ctgaagggcc agcttgcata taattatgaa gatactgctt
     3241 ggattaatac catgataata gaacctgagg tacagcgaaa aggatacgga acagaagcag
     3301 ttaatttgct gatcggatac ctgaaggaga attctaaggt caagaaactc tatctttctg
     3361 tgttggaaga aaattatatg gggaaacatt tctgggagaa gcaaaatttc agcgaaataa
     3421 tgaggattga taagaattct gctaccggtt gcaagcatag gggaatatca attatgtaca
     3481 gacaaatatg acactcctga ttacgatttt ctcaagccaa ttacaggatt ttaccatgag
     3541 cttttcggtt ctgttgggtt catggactca tgcaaatagc cgataaatac tatgtgacac
     3601 cagatttaat tagtgcactt taattatgtc aacttatgct gcaatgttgt ataatagaat
     3661 tactgcatct gcaggcacaa tagtatgttt caccattgtg aaaggctgaa gtatttatgt
     3721 tatagggtac tagtatgaca gtttgacaaa tagcaatcag aaagatataa taattggcat
     3781 aagaaaaatg cgtgaatgct gaacaagatt taatttttta ttttatttgt ttgtaatggg
     3841 gtagaaaaag aaacaggagg ataagtatgt ttaagtctaa gaagttttta ctgtacataa
     3901 cagcgtccgc aatgtcagtt accctttgga caagcacttc
//
LOCUS       DAOOJC010000227         3932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_17866_length_3932_cov_3.566417, whole genome shotgun sequence.
ACCESSION   DAOOJC010000227 DAOOJC010000000
VERSION     DAOOJC010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17866_length_3932_cov_3.566417"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLA01_06880"
     CDS             <1..265
                     /locus_tag="PLA01_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPD01060.1"
                     /translation="VKYLAYDLGSGNIRVNAISAGPIKTLSAKGVKNFGSILDTLPEK
                     APLKRNITQEDLGNTALYLLSDLSGGVTGEVIHVDSGYNIMGL"
     gene            complement(410..1291)
                     /locus_tag="PLA01_06885"
     CDS             complement(410..1291)
                     /locus_tag="PLA01_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HPD01061.1"
                     /translation="MDRGLLIYNPFSGDQRIIQKLDYIIERFQNEGILLQPCRIMTNC
                     EQYISALMAQDRWKYIISSGGDGTLNFVSNIMLKSNVNVPLGIIPTGTCNDFASILNL
                     PTDLKECLDVILEGKTVEVDVGLANEEIYFLSSCAGGIFVDVSFNTDNELKKSFGAFA
                     YYLKAIGEMANMKPFRVSIQTDKEVLEEDILLFFILNGKQAGGFANIVNSADYTDGLM
                     DIVLVKNCNHIDLAGIFFSVLSNSGINNKNVIIITTDRCTINGPEDIVLSIDGEKGPC
                     LPVSVRFINKALKVFTR"
     gene            complement(1314..2435)
                     /gene="glgD"
                     /locus_tag="PLA01_06890"
     CDS             complement(1314..2435)
                     /gene="glgD"
                     /locus_tag="PLA01_06890"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HPD01062.1"
                     /translation="MKSTMGMILTGGKNDRLKELAEMRSSTAVPVGGKYRVIDFVLSN
                     MVNSGITNIGVLTQYSFRSLMDHLGSGKEWDLDRRNEGLFIFPPYLAGEHSGWYQGSA
                     DAMYHNITFLKRSFEEYIVVAQGNCVYKMLFDDMLNYHIKMKADITIAYRDMSDFPVE
                     ELSYMGVMRMDDNGRVVDFKEKHSNPESTICSMGIYILKRELLINLLEECNSLGKYDF
                     VKDVIINKLNTLQIHGYRFDGYWRNLNTINTYYRINMEMLNPEIRRQLFEENGKVYTK
                     VKDEPPAKYNEEAEVTNSIVADGCIVEGTVSNSVLFRGVTIKKNAVVENCIIMQGTVI
                     DEDVKIDSLIIDKNVILSKGINLKGMSQFPIMIEKNAKI"
     gene            complement(2432..3712)
                     /locus_tag="PLA01_06895"
     CDS             complement(2432..3712)
                     /locus_tag="PLA01_06895"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HPD01063.1"
                     /translation="MFNKEIIALLLAGGQGSRLGVLTKNIAKPAVLYGGKYRIIDFSL
                     SNCVNSDIDTVGVLTQYQPLELNAHIGIGKPWDMDRINGGVTILSPYLKAKIGEWYKG
                     TANAVFQNIHYVDKHNPDYVIILSGDHVYKMNYSKMLDFHKENNADATISVINVPWEE
                     AGRYGIMNTDETGKIYEFEEKPQNPKSNLASMGVYIFKWNVLKEYLIRDDKDDESAHD
                     FGKNIIPLMMGEGRSMWAYKFSGYWKDVGTIQAYWESNMDLISRVPDFNLFDPAWKIY
                     TPNPVKPAHYIGPNGSIKKTIAAEGCMIYGKVKNSVISSGVYIGEGSVVEDSIIMTDS
                     YIGSNSYINKCIIGEGVHIGNDVKMGIGEDIPNELKPSLYDSGITVVGERAVVPDGCQ
                     IGKNVVIDAYLTCEEYSSLNIESGKSALKGGKSE"
BASE COUNT         1153 a          919 c          579 g         1281 t
ORIGIN      
        1 cgtaaaatac cttgcttacg atctcggttc cggcaatatc agagtcaatg ccatctctgc
       61 cggacctata aagacacttt cagccaaggg tgtcaagaac tttggaagca ttctggatac
      121 acttccggaa aaagcacctc ttaagagaaa cattacccag gaagacttgg gaaatacagc
      181 tttatacctt cttagtgatt tatccggcgg tgtaaccggt gaagtaattc atgtagacag
      241 cggctataat atcatgggac tttagacacg gcaaacagtg tattaaaaga actcatattc
      301 ctctatagga aaatgagttc ttttttatta acaggctctg tacctgcgaa acttaagttt
      361 ttagaacatt tttcttaact ttcagtggga ttttcgaact gtaaggagtt tatcttgtaa
      421 acactttcag tgccttgttt ataaacctga cactgacagg aaggcacggt cccttctctc
      481 cgtcaatact gagtacgata tcctccggac cgttaatggt acacctgtcg gttgttataa
      541 ttattacatt tttattgtta ataccgctat tactcaaaac gctaaagaat atacctgcca
      601 gatctatatg gttacagttc ttgacaagca ctatgtccat aagcccatcg gtataatcgg
      661 cactgtttac aatattggca aaaccaccgg cctgctttcc gttgagtatg aaaaaaagca
      721 aaatgtcttc ttccaacact tccttatctg tttgaatact aactctaaag ggcttcatat
      781 ttgccatttc gcctatagcc ttaagataat aggcaaaagc accaaaactc tttttcaatt
      841 cattatccgt gttgaaagac acatctacaa agattccccc tgcacatgag cttaaaaaat
      901 atatctcctc atttgcaagt cctacatcaa cctcaacagt ttttccctca agtatgacat
      961 caaggcattc tttaagatcg gtgggtaaat tcagtattga agcaaaatca ttgcacgtcc
     1021 cggtgggaat aatacccaag ggtacattca cattactttt taacatgata ttgctgacga
     1081 agtttagagt accgtctcca ccggatgaaa ttatgtactt ccatctgtcc tgtgccatta
     1141 aagcagaaat gtactgttca caattcgtca taattctgca aggctggaga agaataccct
     1201 cattctgaaa acgttcgatt atataatcga gcttttgtat tatcctttga tcccctgaaa
     1261 agggattgta aattaaaagt cccctatcca tgcctttcta cctctcaaag ccattatatc
     1321 tttgcgttct tctcaatcat aatcggaaac tgtgacatac cttttaagtt tatacccttt
     1381 gacaagataa cattcttatc aataatcaag ctatcaattt taacatcctc atcaattacc
     1441 gtgccctgca ttattatgca attttcaaca acagcattct ttttaattgt aacaccccta
     1501 aacagcacag aatttgatac cgtaccctcc acaatacatc catcggctac tatagaatta
     1561 gtaacctcgg cttcctcgtt atattttgct ggaggctcgt ccttcacctt cgtgtaaacc
     1621 tttccgttct cttcaaagag ctgacgccgg atttcagggt tcaacatttc catattgatt
     1681 ctgtaatagg tgtttattgt attcaggttt ctccagtatc cgtcaaacct gtatccgtgt
     1741 atttgcagtg tatttagctt attaataata acatctttga caaaatcgta ttttccaagg
     1801 gaattgcatt cctccaaaag gtttattagc aattctcttt tcaagatata tatacccatt
     1861 gaacagattg tcgactcagg attgctgtgt ttttctttga aatctacaac tcttccgtta
     1921 tcgtccattc tcattactcc catgtatgac aactcttcaa ccggaaagtc actcatgtcc
     1981 ctataggcaa tagtaatatc cgctttcatt tttatatggt aattaagcat gtcatcaaaa
     2041 agcatcttat acacacaatt gccctgtgct acaacaatat attcttcaaa actccgcttt
     2101 aaaaaagtga tgttatggta catggcatcg gcacttccct gataccaccc cgaatgctca
     2161 ccggcaagat acggaggaaa aataaacaat ccttcatttc tcctgtcaag gtcccactct
     2221 tttcccgatc ctaaatgatc cataagggag cggaaactgt actgagtaag tacaccgata
     2281 tttgttatcc cagaatttac catattcgat aagacaaagt caataactct gtactttcca
     2341 ccaacaggaa ccgcagtgct tgaacgcatt tcagccaact ccttgagcct gtcatttttg
     2401 ccgccggtaa gaatcattcc cattgtactt ttcattcact ctttcctccc tttaaagcac
     2461 tttttccgga ctcaatattc aaagaactgt attcttcgca agtcaaataa gcatctatta
     2521 cgacattctt accgatttga cacccatctg gaacaaccgc tctttcaccc acaactgtta
     2581 tcccggaatc gtatagcgaa ggctttaact cattgggaat atcctcgccg attcccattt
     2641 tgacatcatt gccaatgtgc accccttctc cgataataca cttgttaata tatgaattcg
     2701 aaccgatata gctatccgtc ataataatag aatcctctac cactgatcct tctccaatat
     2761 aaacacccga tgaaatgaca gagtttttaa ccttgccgta aatcatacag ccttcggcag
     2821 ctatcgtctt ctttatactt ccgttaggcc ctatatagtg tgcaggctta acaggattcg
     2881 gtgtatatat cttccatgca gggtcaaaaa gattgaaatc cggcaccctg ctgataagat
     2941 ccatatttga ttcccagtat gcctgtattg ttcccacatc cttccaatac ccgctgaatt
     3001 tataagccca catacttctg ccttctccca tcattaaggg tatgatattc tttccaaaat
     3061 catgagctga ttcatcatct ttatcatccc ttataagata ctcttttaat acattccact
     3121 tgaatatata aacacccatg gatgcgagat tgcttttggg attctggggt ttttcttcaa
     3181 actcatatat tttgcccgtt tcatcggtat tcattattcc atatcttccg gcctcttccc
     3241 atggaacatt tatcacagaa atggtagcat cggcattgtt ttctttgtga aaatcaagca
     3301 tctttgaata gttcatctta tatacatgat ctcccgacag aattataaca taatcgggat
     3361 tatgcttatc aacataatga atattctgaa acaccgcatt cgccgttccc ttataccact
     3421 cacctatttt tgccttcaaa taaggtgaca gtatggtaac tcctccgttt atcctatcca
     3481 tatcccatgg ttttccaatc cctatatggg cattaagctc aaggggttga tattgtgtca
     3541 gcaccccaac cgtatcaata tctgaattaa cacagttact caatgaaaaa tcaattatcc
     3601 tatattttcc tccatacaac acagcaggct ttgcaatatt ctttgtaagt acacccaatc
     3661 tactgccctg ccctcctgca agcaaaaggg caattatctc tttgttaaac atttattttc
     3721 ctcacctcaa tcgatttaag tatacttctg tcacttcttg cttcatcctg tattgctcca
     3781 ctacaaagat tacatttaca tagtacaaaa aacattaaat aatataattt tgttgtaata
     3841 tattctatat tgcagcctgc taatccttta aattatacat aaatatttaa ataatatttc
     3901 caatattaaa cttttaaaac cgttagatta tt
//
LOCUS       DAOOJC010000228         3914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_17957_length_3914_cov_2.922778, whole genome shotgun sequence.
ACCESSION   DAOOJC010000228 DAOOJC010000000
VERSION     DAOOJC010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17957_length_3914_cov_2.922778"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..807
                     /locus_tag="PLA01_06900"
     CDS             136..807
                     /locus_tag="PLA01_06900"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015548923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPD01064.1"
                     /translation="MTKKLFFQAIGKFVFGLVFVALLLFLPAGTLRFWNGWLFIGILF
                     LPISITGIVMMIRNPELLKKRLNAKEKQKEQKTVIFLSGLMFVAAFIVAGLNFRFSWI
                     VLPDWLVYTASGIFVAAYLMYVEVLRENEYLSRTVEVQKNQKVIDTGLYGIVRHPMYF
                     ATLFLFLSMGLVLGSPISFVIMLFYIPIIVKRINNEEKILAQGLEGYEEYRKKVKYRI
                     IPFIW"
     gene            999..2357
                     /locus_tag="PLA01_06905"
     CDS             999..2357
                     /locus_tag="PLA01_06905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPD01065.1"
                     /translation="MLKNKRIRIIIISTLVALVISALYMLGIISDEIKLYKLEATSVL
                     DNQVSSIRNEFMVNINSVYELRALVYGAQGKYVDPQIIGERILKNPNIRNILYAPDSV
                     VTHVYPLEGNEAVIGLDLLSPENKSYEDAKLAAQSDDVILTGPYELKQGGQAFSARLA
                     VRIPDKNSQLKYIGLVSVTMNFPDLISESTNNLHNSLKYDFILEKRLNDSDEYIEIHS
                     GRVSKYDDYISSDFSVSNMDFRLSVVPQSGWYDKSGIMIKIFAFVVVSLAVGIIMGFI
                     FAARQKLIEKSRTDALTGIYNRAGGTHAINTIIKNNLYKSGAFIMMDLDNFKSVNDKL
                     GHQMGDEVLITTAAILKNSIRATDILARLGGDEFMLYLPFEGDSDFLDKKLEEIRIKM
                     HRDIKGTEGVVTVSSSIGVTFYNKDKNFNTLYEQADKALYQSKESGKNRITIYEAKSD
                     IG"
     gene            2498..3844
                     /locus_tag="PLA01_06910"
     CDS             2498..3844
                     /locus_tag="PLA01_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD01066.1"
                     /translation="MFIGREKELSTLNQKIDSDKFEFGIVYGRRRIGKTELLKKVVVK
                     HKAIYYVANEMGLDYNFKQISGVVAKYFDEHFTFESLEALFKYLVQKSKTQRIVIIID
                     EFTYLIKTNPEIMSILQNIIDHLLIDSRLKLIISGSHVGMIEDALAYQKPLYGRATFK
                     MKIEAFDYYDAAKFYPKVSNEDKVRLYSIFGGVPFYNSKIDDNRSVEDNIKTLIIEKG
                     AVFEDEVSFFLSQEVRSVATYGKIINAIASGATRLNDISTKSGSGNTGTTSKYLDLLI
                     QLGIVEREYCFGEGANSRKTIYRIKDRLFNFHYSFIEKHKSQRIIMTPDRFYNTVVKN
                     HLDEYVSLEFEQVCKDFLKRKYKETIEEIGRYWYNDRQEKKDIEIDIVMRANNLLSVY
                     ECKWTDSPVGTKVVNELAKKAKSLDYRSLGFFSKAGYDNQEYKADCYIVDDLFSIN"
BASE COUNT         1291 a          639 c          845 g         1139 t
ORIGIN      
        1 tgccgaacgg cacgtacggt ggtgtgagag ggcagttaat tctgccctac tcgattaacg
       61 gtcaaatgta ttgaggcttg agtaaaataa gttataataa aagcaggata aatagggatt
      121 tgcggaggta aacctatgac gaaaaaactg ttctttcagg caatcggtaa atttgtcttc
      181 gggttggttt ttgtggcatt gcttcttttt ctgcctgccg gaaccttacg attttggaac
      241 gggtggcttt ttatcggtat tcttttcctg ccgatttcta taaccggaat cgtgatgatg
      301 atacgcaatc ctgaactgtt gaagaagcga ctgaatgcaa aagagaagca aaaagaacaa
      361 aaaacagtaa ttttcctaag cggacttatg tttgttgccg ctttcattgt tgcgggattg
      421 aattttcgtt tcagctggat agtgctgccc gattggttgg tatatactgc atccggtata
      481 ttcgtagctg cctatcttat gtatgtcgaa gtgctgcgtg agaatgagta tctgtcgaga
      541 accgtggagg ttcagaaaaa tcaaaaagta attgatactg ggctttacgg aatcgttcgt
      601 catcctatgt actttgcaac tctgtttctg tttctttcaa tgggattggt gctcggatct
      661 cctatttcat ttgtaatcat gttgttctat atccccataa tagtcaagag aataaataac
      721 gaggaaaaga ttttggcaca gggacttgaa ggttatgagg agtacagaaa aaaagtaaaa
      781 tacagaataa ttccgttcat ttggtgatac aaactcagat ttaatctata gattatgtta
      841 taacgccccc taacatgtat ttcctatgca ctaacaatcg tatgaacata tacatagaaa
      901 aattcagggc attgttgtac aataataatt agatttatac acatctttgc gtaaaagcat
      961 acgtaagttt tatttaaagt tcaagaggta ttgtttatat gttgaaaaac aaaagaatca
     1021 gaataataat catttcaacc cttgttgcat tggtaataag tgccttgtac atgctgggca
     1081 taatatccga tgaaataaag ctctataaac tggaggcaac ttccgtactg gataatcagg
     1141 tctcttccat aagaaatgag tttatggtca atataaattc cgtttatgaa ctaagagctc
     1201 tggtgtacgg agcccaaggc aagtatgtgg atccgcaaat aataggtgaa cggatattga
     1261 aaaatcccaa tatccgcaac attttgtatg cgcccgactc tgtggtaaca catgtttatc
     1321 ctttggaagg caatgaagcc gttatcggat tggacttgct aagtcccgaa aacaaaagtt
     1381 atgaggatgc taaattggca gcacagtctg atgatgtaat tcttacggga ccgtatgaat
     1441 taaagcaagg cggacaagcc ttctccgcaa gactggcggt caggattccc gataaaaatt
     1501 cccaattgaa gtatatcgga ttggtatcgg taaccatgaa tttccccgat ttaatatccg
     1561 aaagtacaaa taatttgcat aacagtttaa aatatgattt tatactggaa aaaagactga
     1621 atgacagtga tgaatatatc gaaatccatt cgggcagagt ttcgaaatat gacgattata
     1681 tatcttccga cttttccgta tccaacatgg acttcagatt atccgtagta ccgcaatccg
     1741 gttggtatga taagtcggga ataatgatta aaatatttgc ctttgttgtc gtgtctcttg
     1801 ctgtaggtat aatcatgggc tttatcttcg ccgccaggca gaaactaata gaaaaatcca
     1861 gaaccgatgc actgacggga atatataaca gagcgggcgg aactcacgca ataaatacta
     1921 taattaaaaa caatctctat aaaagcggag cctttataat gatggacttg gacaacttca
     1981 agagtgtcaa tgacaaactg gggcatcaga tgggggatga ggtacttata accacggccg
     2041 caattctgaa aaattccatc cgtgccacgg atatattggc cagactggga ggagatgagt
     2101 ttatgctgta ccttccgttt gagggcgact ccgatttctt agataaaaag cttgaggaaa
     2161 ttcgtattaa gatgcaccgc gacatcaaag gcacggaggg agtcgtgacc gtaagcagca
     2221 gtataggcgt aaccttttat aacaaagaca aaaattttaa taccctttat gagcaagccg
     2281 ataaagccct ctatcagtca aaggaaagcg ggaaaaaccg gataacgata tatgaagcaa
     2341 aatcagacat cggatagcga tttatcgggg attagtctct ttatataagt atagattaca
     2401 tgcttttaag ttatgttttt gggcttgtat tataaaccgc attatgataa actgtattac
     2461 gataaagttt atcgtgatac agcaaaggag ggttcgtatg tttatcggaa gagaaaaaga
     2521 gttaagtaca ctcaatcaaa aaattgattc agataaattt gaattcggta ttgtttacgg
     2581 tcgcaggcga atcgggaaga cggagttact taagaaagtt gtggtaaagc acaaagcaat
     2641 ttactatgtt gccaatgaaa tgggtcttga ctataacttt aaacagataa gcggtgtagt
     2701 ggcgaaatat tttgatgaac actttacttt cgaatcattg gaagcattat ttaagtatct
     2761 tgttcaaaag agcaaaactc aaaggatagt aataataata gatgaattta catatttgat
     2821 aaaaacaaat cctgagatta tgtctattct tcagaatatc attgatcatt tattgataga
     2881 ttcaaggttg aaattaatta tttcaggaag ccatgtggga atgatagaag atgctttagc
     2941 atatcaaaag ccgttgtacg gcagggccac attcaaaatg aaaatagaag cttttgacta
     3001 ttatgatgcg gcaaaatttt atccgaaggt ctcgaatgaa gataaggtca ggttatacag
     3061 catttttggc ggtgttccgt tttacaacag caagatagat gataatagaa gtgttgaaga
     3121 taatattaag actcttatca ttgagaaagg agctgttttt gaagatgaag tttctttctt
     3181 cttaagtcag gaagtcagaa gtgtagctac atacggcaag attattaatg ctatcgcatc
     3241 cggtgcaaca cggcttaacg atatttctac caagagcggt tccggtaata ccggtacaac
     3301 atccaagtat ctggacctct tgatacagtt aggcatagta gagcgggaat attgtttcgg
     3361 tgagggggca aattccagga aaacaatcta tcgtataaaa gaccgtttgt ttaattttca
     3421 ctacagtttt attgagaagc acaagtcaca gagaataata atgactcccg accgcttcta
     3481 taacactgtg gttaaaaacc atctggacga gtatgtttca ttggaatttg agcaggtttg
     3541 taaagacttt ttgaagcgga aatataaaga aactattgaa gaaatcggac gttactggta
     3601 caacgacaga caggaaaaga aagatatcga gatagatatc gtaatgaggg cgaacaactt
     3661 gttatctgtc tacgagtgta aatggacaga tagtcctgtc ggaaccaaag tcgtgaatga
     3721 gcttgcaaaa aaagctaaaa gtttagacta ccggagctta ggttttttct cgaaagccgg
     3781 ctatgacaac caggaatata aggcggactg ttatattgtt gacgatctgt tttccataaa
     3841 ttaaaaagga gtgacaacca catggatatt aaaagaatta aaatagagta tatgcctaaa
     3901 ataaccgatg acca
//
LOCUS       DAOOJC010000229         3895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_18064_length_3895_cov_3.954427, whole genome shotgun sequence.
ACCESSION   DAOOJC010000229 DAOOJC010000000
VERSION     DAOOJC010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18064_length_3895_cov_3.954427"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..856)
                     /locus_tag="PLA01_06915"
     CDS             complement(20..856)
                     /locus_tag="PLA01_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HPD01067.1"
                     /translation="MLEIISCSRRTDIPAFYYDWLQECLKNKYAMVKNPYNGSAYIVD
                     LSCEKVHSICLWSKSFANVLKDPKYLSMYNLYFQFTITGYSRVLEPNVVDTNEAVKQM
                     EQLALRYSPQQINWRFDPIVFSKEGEKEPVPDNLERARLKMFETLCRDISSFGVNRCT
                     VSFLCLYKKVEQRMKKSNFNCSVPSQQKQIEFVSQLVETADKFGVTVYACSSPVIEGV
                     SGVKKGHCIDGAYLEALFGKRASRAKDAGQRKECGCTRSRDIGGYSGQSCHHGCIYCY
                     AQ"
     gene            complement(1091..1783)
                     /locus_tag="PLA01_06920"
     CDS             complement(1091..1783)
                     /locus_tag="PLA01_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPD01068.1"
                     /translation="MIISGIQKNSFVDFPGKLSAVVFTPGCNMNCFYCHNRMLISGGN
                     SEELVDEDSFLNMLSDRKGFLDGVVVSGGEPTLQKDLGDFIKKVKKLGYPVKLDSNGT
                     NPKVIEGLIDKGLVDYIAMDIKAPFDKYSEVCGTDVDIAQIKRSIEILKRGIVAYEFR
                     TTVAPGLDMDDICEIARSIAGARLYVLQKCREIKGVDLRISYSPQFLLDAAIRIKGIV
                     NRVETRGVAVSA"
     gene            complement(1780..3870)
                     /locus_tag="PLA01_06925"
     CDS             complement(1780..3870)
                     /locus_tag="PLA01_06925"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HPD01069.1"
                     /translation="MITKISKRDGRYERYQPEKITWAIFSAAAACGGNDFAKAEELCR
                     QVDEILQKLYSDSIPGVEEIQDIVEKVLIENGHAKTAKAYILYREKRKSARDLNALVG
                     ATIDLFTSYLGDRDWHIQENANTQKSINGLNNYVRETFTKKYWLHEIYPIEVREAHEN
                     GDCHIHDLGFFGPYCAGWDLRQLLLEGFGGVEGKLESKPAKHLRSFLGQIVNSTFTTQ
                     GETAGAQAWSSFDTYCAPFIRHDNMTYEQVRQCLQEFIFNINVPTRVGFQCPFSNLTF
                     DIKVPNTLKDEPVIIGGKYTEHTYGEFQKEMDIFNKAFCDVMLEGDAKGRVFTFPIPT
                     INITKDFDWDNPVVDEFMRITCKYGIPYFANYVNSDLSPEDAVSMCCRLRIDTTELRK
                     RGGGLFGSNPLTGSIGVFTINLPRIGYLSNSESEFKARLWKIANIGKTSLEIKRKIIE
                     QQSEKGLYPYSIHFLRNVKKRTGQYWFNHFNTIGIVGMNEALENFMGKDLTTQEGQKF
                     AIDIMNYLRDILIDFQNETGHFYNLEATPAEGTSYRLAKLDRERYPDIITAGKETPYY
                     TNSSQLPVEYTDDIFEVLELQDELQSLYTGGTVQHLYLGESIEDIEVCKRLIQKVFTN
                     YKMPYISITPTFSICNEHGYIKGEHFKCPKCGSETEVWSRVTGYLRPVKNYNIGKKEE
                     YFTRKKFKLVEKVS"
BASE COUNT         1057 a          948 c          654 g         1236 t
ORIGIN      
        1 atatcttttg acaactcgat tactgtgcat agcaataaat acacccatga tggcaactct
       61 gtccgctata tcctccaata tctctcgacc gggtacatcc gcattctttt ctttgccctg
      121 cgtcttttgc acgggatgcc cttttgccaa acaatgcttc aagataagct ccgtctatac
      181 aatgtccctt cttcacccct gatacccctt caataaccgg actgctgcat gcataaacag
      241 taactccgaa tttatcggca gtctcaacta attggcttac aaattcaatt tgcttttgct
      301 gtgaaggaac tgaacaatta aaattggact ttttcattct ttgttctact tttttataca
      361 ggcaaagaaa acttacggta caacggttta cgccaaagga tgaaatatcc ctgcataaag
      421 tctcaaacat ctttaaccta gccctctcaa ggttatccgg taccggttcc ttttctcctt
      481 ctttactaaa aacaataggg tcaaatctcc agttaatttg ctgaggagaa taccttagtg
      541 ccagttgttc catctgcttg actgcctcat tggtatcaac aacatttggt tctaagactc
      601 ttgaatatcc tgtgattgta aactgaaagt acaaattata catcgacaaa tatttaggat
      661 ctttaagaac attggcaaat gactttgacc acaagcaaat tgagtgcacc ttttcacaag
      721 ataaatccac tatgtatgca gacccgttgt agggattttt taccattgca tatttatttt
      781 tcagacattc ctgcagccag tcataataaa aggccggaat atccgttctt ctgctgcagc
      841 ttattatttc aagcatcgga tcccaaattc ctccacaaag tatttgtcgc tatatcaaag
      901 tttactgttt aactatttta ccacagttta ccttataatc aaagtaagta aaggaatttt
      961 aaaaaaacta aggcagaata ccaccttagt taattaatgt atataaaact ctacaaactt
     1021 aatctttcat gttttgtcgc ctgtaaattt gcttattgtg cattcttctt tacccaaata
     1081 acaggaaccg ctatgccgat acagcaacac ctcttgtttc aactctgttt acaattccct
     1141 taattctaat cgccgcatcc agcaaaaact gcggactgta cgaaatcctc aggtccaccc
     1201 cttttatctc cctgcatttt tgcaagacat acagccttgc accagcaata ctcctcgcaa
     1261 tttcgcaaat gtcatccata tcaagtcccg gtgcaacggt ggtacggaat tcatatgcca
     1321 cgatacctct ttttaagatt tctatgctcc tctttatttg ggcaatgtcc acatctgtgc
     1381 cgcagacttc gctgtacttg tcaaatgggg ctttgatatc catggcgata tagtcaacca
     1441 aacctttatc gataaggcct tcaatcacct tgggattagt accgttggaa tccaacttaa
     1501 ccggataacc caacttctta acctttttta tgaaatctcc caagtccttt tggagggtgg
     1561 gctctccacc gctaacaacc acgccgtcca aaaagccctt cctatccgaa agcatattca
     1621 aaaacgaatc ttcatccacc agttcttcac tattgccacc ggagatgagc attctgttgt
     1681 ggcagtaaaa gcaattcata ttacagcccg gtgtaaaaac aacagctgac agttttcccg
     1741 ggaaatctac aaatgaattc ttttgaattc cggaaattat catgatacct tctccacaag
     1801 tttaaatttc ttccgcgtaa aatactcttc ctttttacct atgttatagt tctttaccgg
     1861 cctcaaatat ccggtcaccc ttgaccatac ttccgtttcg ctgccgcact tcggacattt
     1921 gaaatgctct ccctttatat agccgtgttc gttacagata ctaaaggtgg gcgtaataga
     1981 tatatacggc attttatagt ttgtaaacac cttttgtata agccgtttac acacctcaat
     2041 atcctcaatg ctttctccaa ggtaaaggtg ttgaaccgtt ccgcccgtat acaatgattg
     2101 cagctcatcc tgaagctcca aaacctcaaa aatgtcgtcg gtgtattcca ccggaagctg
     2161 cgatgaattt gtataataag gagtttcttt tccggcggta attatatccg gatatctttc
     2221 cctgtccaac tttgccagtc tgtaggatgt tccctctgcc ggagttgcct caagattata
     2281 aaaatgccct gtttcgttct ggaagtcaat taaaatatct ctcaaataat tcataatatc
     2341 tatggcaaac ttctgtccct cctgggttgt gaggtccttc cccataaagt tttccaacgc
     2401 ttcattcatg cctactattc caatggtatt gaaatgattg aaccagtatt gtcctgttct
     2461 tttcttaaca tttctgagaa aatgtatcga ataagggtac aatccctttt ccgactgctg
     2521 ttcaattatc tttctcttaa tctccagcga tgtttttccg atattcgcaa tcttccaaag
     2581 ccttgccttg aactcgctct ccgaatttga caaataacct atcctcggaa ggttaattgt
     2641 gaaaacccct atggaaccag tcaggggatt gctgccaaaa agtcctcccc ctcttttacg
     2701 aagctcagtg gtatcaattc tgagacggca gcacatggaa accgcatcct ccggagaaag
     2761 atcggaattt acatagttcg caaaatatgg tatgccgtat ttgcatgtaa ttctcataaa
     2821 ctcgtccacc acagggttgt cccaatcaaa atccttcgtt atgtttattg taggtatggg
     2881 gaaagtaaac acccttccct ttgcatcacc ctcaagcatg acatcgcaaa atgccttgtt
     2941 gaatatgtcc atttcctttt gaaactcgcc ataggtatgt tcggtgtact ttccaccgat
     3001 tataaccggc tcgtccttta aggtgttggg aacttttata tcaaacgtga ggttggaaaa
     3061 gggacattga aatcctaccc ttgtaggaac atttatatta aaaataaact cctgaaggca
     3121 ttgacgaact tgttcatacg tcatgttgtc atgacgtatg aacggtgcac aataggtgtc
     3181 aaaactggac catgcctgtg caccagctgt ctccccctgg gttgtaaatg ttgaatttac
     3241 tatttgtcct aaaaaggacc gcaaatgctt tgcaggtttg ctctcaagct ttccttcaac
     3301 gccaccgaat ccttctaata acagctgacg caaatcccac ccggcacagt acggaccgaa
     3361 aaagccaagg tcatgtatat ggcagtcccc attttcatgg gcctccctga cctctatggg
     3421 gtaaatttca tggagccaat atttttttgt aaaggtctcc cttacgtaat tgttaagacc
     3481 gttaatgctt ttttgagtgt tggcgttctc ctggatatgc caatccctat ctccaagata
     3541 gctggtaaac aaatcaatgg tagcacccac caatgcgttc aggtcgcggg cactcttcct
     3601 tttctcacgg tataatatgt atgcctttgc tgtcttggca tgaccgtttt caatcaaaac
     3661 cttttcaaca atatcctgaa tttcctccac accgggtata ctatccgaat acagcttctg
     3721 taagatttca tccacctgac ggcaaagttc ttctgccttg gcaaaatcgt tgcctccgca
     3781 ggctgcagca gcactaaaaa ttgcccaggt tattttttca ggctggtatc tttcgtacct
     3841 tccgtctctc tttgatattt ttgtaatcat ttagacatct cctatttatt tttct
//
LOCUS       DAOOJC010000230         3894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_18077_length_3894_cov_3.383173, whole genome shotgun sequence.
ACCESSION   DAOOJC010000230 DAOOJC010000000
VERSION     DAOOJC010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18077_length_3894_cov_3.383173"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PLA01_06930"
     CDS             <1..524
                     /locus_tag="PLA01_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPD01070.1"
                     /translation="APDLSKKEGIRDKAMLELLYATGIRVSELISLDLSDVNLEMRYI
                     MCNKGTRERMIPIGSLSVNAMRNYLTKSRNLLIQRNDEKALFVNVNGKRLTRQGFWKI
                     IKQYKNQAKINKDITPHTLRHSFAAHLLENGADLRSIQEMLGHSDISSTQVYAQIAKN
                     RLKEVYKKTHPRA"
     gene            686..1852
                     /locus_tag="PLA01_06935"
     CDS             686..1852
                     /locus_tag="PLA01_06935"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HPD01071.1"
                     /translation="MKRAIIIVLDSVGIGELPDAQKYGDKGSNTLGNIAANIPGFSLP
                     NLESLGLGNIDNIKGIKLAENPIGCYGRMAERSAGKDTTTGHWEIAGIIMDRPFPVYP
                     DGFPPEIIDKFEKSINTKIIGNFAASGTEIINMLGDKHVKTGYPIVYTSADSVFQIAA
                     HEEIIPVQRLYEMCEIARSILTGEHAVGRVIARPFAGKSGEYKRTDRRRDFSLDPIGK
                     TVLDYVKETGYEVKAVGKIEDIFNKKGITDAVHIHDNMDGVDKTLQYMKEDFEGILFT
                     NLVDFDMLYGHRNDIEGYAKALMEFDGRVTEILSCLKDDDILFITADHGCDPSTESTD
                     HSREYVPLIVYGKNLKRGINIGTRSTFSDIAQTVAQYLGIDAGFKATSFLEQIL"
     gene            1895..3196
                     /locus_tag="PLA01_06940"
     CDS             1895..3196
                     /locus_tag="PLA01_06940"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HPD01072.1"
                     /translation="MRMIDLINIKKHGEVLTAAEIDYIVQGYTKGKIPDYQMSAFLMA
                     VYFKGMSPEEISNLTMSMVNSGEVVDLSGIEGVKVDKHSSGGVGDKLSLIIIPLCASL
                     GIPVAKMSGRGLGHTGGTIDKLESIEGFRTELSKEEFINNLNTHKMAIVGQAQNLTPA
                     DKKIYALRDVTGTVDSIPLIASSIMSKKIASGCDRIVLDVKVGSGAFMKSVDEAVVLA
                     KTMVDIGKSLGKTTVAVVTDMNQPLGYEVGNANEVREAIDVLKGNGAVDVTVIALTIA
                     SYMAVLAGSYPDFKSAYNSMNELIKSGKALEKFKELVKIQGGNVGIVENPDLLPQAKN
                     HIEIKAEKSGFINSIDAESIGVSAMLLGAGRRTKEDRIDPSAGITIVRKVGDEVKSGD
                     TLCVLHTNMQECNDAQKLSHNAFVIKDEMPAPIKYVYEVVE"
     gene            3349..>3894
                     /locus_tag="PLA01_06945"
     CDS             3349..>3894
                     /locus_tag="PLA01_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPD01073.1"
                     /translation="MLKNIFSIFLAVILLLNIAAIPVLAESDLDEPVYDVEAVAQITS
                     NTNIFELKAKAHVLMDATTGQVLLEGNSHERMPIASVTKIMSMLLIMEAIDSGRINMD
                     DIVTASDYASGMGGSQAYIEPGEQYSVRDALKAVATHSSNDVTVSLAELVAGSEQGFV
                     VLMNEKAKELGMNDTNFLDCTG"
BASE COUNT         1259 a          651 c          947 g         1037 t
ORIGIN      
        1 ccgcgccgga tctttcaaaa aaggaaggca taagggataa ggccatgctt gagcttctct
       61 atgctaccgg tataagggta tcagagctta tttctctgga tctgtcggat gtaaaccttg
      121 aaatgcgata tataatgtgt aacaagggta caagggagag aatgattccc attggttcat
      181 tgtcggtgaa tgccatgagg aactatctaa ccaaatccag gaacctcttg atacaaagaa
      241 atgatgaaaa ggctttgttt gtcaacgtga acggaaaaag acttacacgg caagggtttt
      301 ggaaaataat caagcaatat aagaatcagg ctaaaattaa taaggatatt actcctcata
      361 ctttgaggca ttcctttgcg gctcatcttt tggaaaacgg agctgattta agatccatac
      421 aggaaatgct gggacattcc gacatatctt caacgcaggt ttatgctcag attgcgaaaa
      481 accggttgaa ggaggtatat aaaaagactc atccaagagc gtagaatttt ggcgggtgta
      541 aattgaattc tttgtattgt ttgaattttg tattataatg aaaaggtaag atgattctat
      601 cttgcctttt ttagttaccg gttttattga gaagaaagga tgtaaatcgc gggtctttaa
      661 ataatcagca aaggagtagt ggtctatgaa aagagcaata atcattgttt tggacagtgt
      721 tggaatcgga gagcttcccg atgcacaaaa atacggcgat aaaggaagca acactttggg
      781 aaacatagca gccaatatcc cgggtttttc ccttccaaat cttgaatcac tgggtcttgg
      841 caatattgat aacataaaag gaattaaact ggcagaaaat cctatcggat gctatggaag
      901 aatggctgaa aggtctgccg gaaaggatac cactacagga cactgggaga ttgcaggcat
      961 aattatggac aggccgtttc ctgtatatcc cgatgggttt cctcccgaaa taatcgacaa
     1021 attcgaaaaa tctataaata ctaagattat tgggaatttt gcagcttcag ggacagaaat
     1081 aataaacatg ttaggagata agcatgtaaa gacaggctat ccaatcgtgt acacttctgc
     1141 agacagcgta tttcaaatag ctgctcatga ggaaataatt ccggtacaaa gactttacga
     1201 aatgtgcgaa attgcaagaa gcattttaac cggagaacat gcagtgggaa gagtaattgc
     1261 caggccgttt gccggtaagt cgggagaata taaaagaacc gacagaagaa gagatttttc
     1321 ccttgacccg atcgggaaaa ctgttttgga ctatgtgaaa gagacgggtt atgaggttaa
     1381 agccgttgga aaaattgaag atatatttaa caaaaagggt attaccgatg cggtccacat
     1441 ccacgataac atggacggcg tcgataaaac ccttcagtat atgaaggagg actttgaagg
     1501 tattctgttt acgaatcttg tggattttga catgctctat gggcaccgca acgatataga
     1561 gggatatgcg aaggcactaa tggagtttga cggtagagta acggaaatac tatcgtgcct
     1621 taaggatgat gatattttat ttatcactgc agaccatggc tgcgatcctt caacggaaag
     1681 tacagaccac tcaagggagt acgttcctct tattgtatac gggaaaaatt taaagagggg
     1741 tataaatatc ggaacaagaa gcactttttc cgatattgca caaaccgttg cccaatacct
     1801 tggaatagat gccgggttta aagctacaag ttttctggaa caaattctgt aaatgatgta
     1861 agaacagaaa atacactatt tgggggttat gtcaatgaga atgatcgatc tgataaatat
     1921 aaagaagcat ggtgaagtcc tgacagcagc tgaaatcgat tatatagttc agggctatac
     1981 caagggtaaa atccccgatt atcaaatgtc agccttttta atggcggttt acttcaaggg
     2041 aatgagcccg gaggaaattt cgaaccttac aatgtcaatg gtgaattccg gagaagtagt
     2101 tgacctttcc ggtattgagg gagttaaggt tgacaaacac tcctcggggg gagtgggaga
     2161 caagcttagc ctaataataa ttcccttatg tgcgagcctg ggaattcctg tagcaaagat
     2221 gtccggccgg ggattgggac acaccggcgg gactattgac aagcttgagt caatagaagg
     2281 gtttagaaca gagctttcca aggaagaatt cattaataat ttgaacacgc ataaaatggc
     2341 aatagtcggt caggctcaaa acctaactcc ggcagataag aagatatatg cccttaggga
     2401 tgttacgggt actgttgaca gtattcccct tattgcaagc tcaataatga gcaaaaagat
     2461 agcttcggga tgtgaccgaa tagtattgga tgtaaaggtg ggatccgggg ccttcatgaa
     2521 atctgttgat gaagcagtgg ttcttgcaaa aaccatggtg gatatcggca aatcccttgg
     2581 aaaaacaacc gttgctgttg ttaccgacat gaatcagccc cttgggtatg aggtaggaaa
     2641 tgccaatgaa gtaagggaag caatagatgt acttaaaggc aacggtgccg tggacgtaac
     2701 tgtcatagca cttacgattg catcttatat ggctgttttg gcaggctcat atcctgattt
     2761 taaatcggca tacaacagta tgaacgagtt gattaaatcg gggaaagcac tggaaaagtt
     2821 taaggaactt gtaaaaatac agggcggaaa tgtggggatt gtcgaaaacc ccgatttact
     2881 gccgcaggca aaaaatcata tagaaataaa ggctgaaaaa agcggattta tcaattcaat
     2941 agatgccgaa agcataggtg tatcggcgat gcttcttggt gccggcagaa gaactaaaga
     3001 ggacagaata gacccttcgg caggtataac aattgtacgc aaggttgggg atgaggttaa
     3061 aagcggtgat accttgtgcg tacttcatac aaatatgcag gagtgtaacg atgcacaaaa
     3121 actgtctcat aatgcttttg tgataaagga tgagatgcct gcaccgatta agtatgtata
     3181 cgaagttgta gagtgaaatg aggaaaatta ggaagaaata ttgcaaagta agttttagcc
     3241 gcctgcaaat attcctggaa cttttgattt gcaacttaaa atttagtata tccaagcatt
     3301 attctgcata aaaatctaat aatgataaat gcttggaggg gtccttaagt gttgaaaaat
     3361 attttcagta tttttttagc agttatttta ttattaaaca tagcggcaat acctgttttg
     3421 gccgaatctg accttgatga accggtttac gatgttgagg ccgttgcaca gataacgtca
     3481 aatacgaata tttttgaatt aaaggcaaaa gcccatgtct tgatggatgc gacgacaggc
     3541 caggttcttt tggagggaaa cagccacgaa agaatgccta ttgcgagtgt aaccaagatt
     3601 atgtccatgc ttttgataat ggaagctatt gattcgggga gaattaatat ggatgacatt
     3661 gttactgcat cggattatgc atcgggaatg ggaggatcgc aggcctatat cgagccgggg
     3721 gaacaatatt ctgtcagaga tgctttaaaa gcggttgcaa ctcactcatc caatgacgta
     3781 accgtttcat tggcagagct ggttgcggga agtgaacagg gctttgtggt tcttatgaat
     3841 gaaaaggcaa aggagcttgg aatgaatgac accaatttcc ttgattgtac ggga
//
LOCUS       DAOOJC010000231         3891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_18094_length_3891_cov_3.425182, whole genome shotgun sequence.
ACCESSION   DAOOJC010000231 DAOOJC010000000
VERSION     DAOOJC010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18094_length_3891_cov_3.425182"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="PLA01_06950"
     CDS             <1..164
                     /locus_tag="PLA01_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPD01074.1"
                     /translation="KVVLVGFGTFEVRQRAARKGRNPQTKQEITIPASKAPVFKAGKG
                     LKEIINKKK"
     gene            complement(275..1090)
                     /gene="spo0A"
                     /locus_tag="PLA01_06955"
     CDS             complement(275..1090)
                     /gene="spo0A"
                     /locus_tag="PLA01_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003521086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HPD01075.1"
                     /translation="MKGEISVLIADDNYEFAGLLKEYMEQFEDIEVVEIAKDGLQALE
                     LIVSLKPDVVVLDIIMPNLDGLGVLEKLSSMQIDFKPMFIMLTAIGQDVFVQRAVALG
                     AEYYIIKPFDADVLVKRIRQLYKEKYMSAFSNKPVVKNSELINSSTNVDRTYDIEVEV
                     TNLMHEVGIPPHMAGYQYLREAIIQTVINAKAFTSITKTLYPEVAKKYNTTSQKVERA
                     IRNAIESAWARGNPDTMDSLFGYTFSTSKGKPTNSECIAMMADRIRLTMNIRQ"
     gene            complement(1347..2618)
                     /gene="aroA"
                     /locus_tag="PLA01_06960"
     CDS             complement(1347..2618)
                     /gene="aroA"
                     /locus_tag="PLA01_06960"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPD01076.1"
                     /translation="MLLRVRKSKASGRIMIPGSKSHTIRALFLGALAKGKSEIRNPLV
                     SSDALSAVEVCRALGAKINHLEDRYVIEGFGANPKTPMDVVNVGNSGTTLRFGVMTAA
                     LGEGYSVFTGDDQIRKRPLDPLLCAIGNLGAQVFSTRGNGIAPVVVKGRLKGGSTELD
                     SITSQYLSSILINAPVIDSDTEVILTRLNERPYVDMTMWWLDKLGIRYLNHDYKTFYI
                     KGGQSYNPFDATIPGDFSSASFFAVQAAISGEEFILDNLDMTDPQGDKEVFAILEDMG
                     AKVRIREGSVIVKGEELTGREIDMNAIPDALPVMAVAGCFAKGETRLVNVPQARLKET
                     DRIHVMCEQLGSMGADIEELEDGLVIRESRLKGCRVNGCYDHRVVMALAVAGLNAQGE
                     TIIDTAEAINITFPDFVGLMSESGADMSLYN"
     gene            2905..3828
                     /locus_tag="PLA01_06965"
     CDS             2905..3828
                     /locus_tag="PLA01_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD01077.1"
                     /translation="MNGTVLLIEYSEYEREKIKIVFDNIGEYEFIEIDSLKKLYKTIS
                     SLDSVTLVIMNLEFPVENEGLETLSLLRKNSSTANTPVIIVTKSDNAVYRHAVQKFKV
                     RDFIIKPYQTKRLENSIRSILRIEQNFRYEFDSANVITMSIEDYISKEFKIASRAGQN
                     LSIILITPIDVKKETADQTKITPELKEQIYKIVVEKVKTLSRSTDTALLNGNKDILVI
                     LPFTDSSGAQKVLEKIKNSVEEGLRQLGINYSDYFYSVSATFPNDGKSFQNLMEKAVK
                     KVEDKIMLEKITSIAADTMDNARKTYKKYKL"
BASE COUNT         1273 a          803 c          619 g         1196 t
ORIGIN      
        1 acaaggtagt tcttgttggt ttcggaacct ttgaggttag acaaagagct gcaaggaagg
       61 gaagaaaccc acaaacaaaa caagaaatca caatccctgc atcaaaagca cctgtattta
      121 aagcaggtaa aggattaaaa gagattatta acaagaagaa ataaatctct attttctagt
      181 tgtaggataa ttaataatta attattaaag acctatcatt gtgataggtc tttaataatt
      241 gtcagcttta acccaaatta tcccgctttt taaattattg ccttatgttc atagttaacc
      301 taatcctgtc agccatcatt gcaatacact cagaatttgt aggcttacct ttactggtac
      361 taaatgtata tccgaataac gagtccattg tatcgggatt tccccttgcc caagcactct
      421 caatagcatt tcttattgct ctttcgactt tttgtgatgt agtgttatac tttttagcca
      481 cttcaggata caaagtcttt gtaatagaag taaaagcctt ggcgtttata accgtctgga
      541 taatagcctc gcgtaaatac tgatatcccg ccatatgagg cggaatacct acctcatgca
      601 ttagatttgt aacctccacc tctatgtcat aggttctgtc aacattagta ctggagttta
      661 tcagctctga atttttcacg acaggcttat ttgaaaaagc tgacatatac ttctccttat
      721 acaattgacg gatcctttta accaaaacgt ctgcatcaaa aggctttatt atgtaatatt
      781 cagcaccaag tgcaactgct ctttgaacaa aaacatcctg accgattgca gtaagcatga
      841 taaacatagg tttaaaatct atttgcatgg aagaaagctt ttctaaaact cccaatccat
      901 ccaaattagg cataataata tcaagcacta ccacatcagg ctttaatgac acaatcagtt
      961 ctaaagcctg taaaccgtcc ttagcaattt ctacaacctc aatgtcttca aattgctcca
     1021 tatactcttt tagtagacct gcaaactcat aattgtcatc agctatcaaa acagaaattt
     1081 cgcccttcat ctcattttcc gccttttcta aattaataaa aaagaataat gctaaattgt
     1141 gctatgattt tcgtgaaaaa ctcttttcac ttacttattc aaattttcgc gaaatcacat
     1201 gctataacct gttgaacatg tctggtaaat gtaatctgcc tattatattt atattttttt
     1261 aaaccttaga agctataagt cctaaaaata atctccaatt ctattataat ataaaattgt
     1321 ataaaaatat acttattttg ctaatatcag ttgtataaac tcatatctgc cccggattca
     1381 gacattaatc ctacaaaatc cgggaaagtt atattaatgg cttcagctgt gtctattatt
     1441 gtctcccctt gtgcatttag tcccgcaacg gccaatgcca ttactaccct gtggtcatag
     1501 cacccattga ccctgcatcc ttttaagcgg ctttcacgaa tgacaagtcc atcctcaagt
     1561 tcctctatgt ccgctcccat actaccaagc tgttcgcaca taacatgaat cctatcagtc
     1621 tccttaagcc ttgcctgcgg tacgtttaca agtctggtct ctccttttgc aaagcatcct
     1681 gcaacagcca taaccggaag tgcatccggt attgcattca tgtcgatttc ccttcctgtt
     1741 agctcctctc ccttaacaat gacgctccct tcccgtatcc taacctttgc ccccatatct
     1801 tcaagtatgg caaaaacctc cttatctccc tggggatccg tcatatccag attgtccaga
     1861 ataaattcct caccggatat ggcagcctga acggcaaaaa agctggctga agaaaaatct
     1921 cccggaatgg tagcatcaaa aggattgtag ctttgccctc cctttatgta aaaagtttta
     1981 tagtcgtggt ttaaatatct aatccccagc ttgtcaagcc accacatggt catatctaca
     2041 taaggccgtt cattaagcct tgtaaggatt acttccgtat ctgaatcaat aacaggtgca
     2101 tttatgagta tggatgagag atactgagac gtaatagagt caagctccgt gctgcccccc
     2161 ttaagccttc ctttaacaac tacaggtgcg attccgtttc cccttgttga aaaaacctgt
     2221 gcaccaaggt ttccaattgc acacagtaac gggtccaagg gtctttttct gatctgatca
     2281 tcgcctgtaa aaactgaata accctcaccc agtgcggctg tcattactcc gaatcttaga
     2341 gtagtgcctg aattacctac atttacaaca tccataggag tctttggatt cgctccaaag
     2401 ccctcaataa catatctgtc ttcaagatga ttaatcttcg cccctaaagc cctacaaacc
     2461 tctacagcag acaatgcatc gctggaaaca agaggattcc tgatttccga tttacctttg
     2521 gcaagggctc ctaaaaataa cgcccttatg gtatgggact ttgaccccgg aatcataatc
     2581 ctgccagagg cttttgattt tctaactctt aataacataa caataaccct ttctaccgta
     2641 ctaaattgca ttataataat acattgttta attaaaatta acaactttag gttataaata
     2701 aaaaaacata tccattgcct gtacatgaat actctatata tattttaata taaaatggca
     2761 cttaaataaa tattcaatat tttttatagg aagaactttt atattatcaa atattctata
     2821 gctttgggta aacatacagt tttaaacttt ccaattttgt gttaaaatag ttttacaact
     2881 aaaaaataat gtcaggtatt gataatgaat ggtactgtat tattaattga atactctgag
     2941 tatgaaaggg aaaaaatcaa aatagtattt gataacatcg gagagtatga gtttattgaa
     3001 atagatagcc tgaaaaagct ctacaagact atctcaagct tggactctgt aactctggta
     3061 attatgaatt tggaattccc tgtagaaaat gaaggccttg agaccctgtc actacttagg
     3121 aaaaactcat caacagccaa cactcctgtt attattgtta caaaatccga taatgcagta
     3181 taccgtcatg ctgttcaaaa gtttaaagta agggacttta taattaagcc ctatcaaaca
     3241 aaacgacttg agaattccat aaggagtata ttaagaattg agcaaaattt ccggtatgaa
     3301 tttgacagtg caaatgtgat tacaatgtcc attgaagact atatatccaa ggagtttaaa
     3361 atagcctcaa gagcaggtca gaatctgtcc ataatcctta ttactcctat agatgtgaaa
     3421 aaggaaaccg cagatcaaac caagataaca ccggaattaa aggagcaaat atataaaata
     3481 gttgttgaaa aggtcaagac cctttcaaga tcaactgata cggctctttt aaacggaaac
     3541 aaggacatcc ttgttatatt gccctttact gattcttccg gagcacaaaa ggttttagaa
     3601 aaaatcaaaa acagtgttga agagggtctt agacaactgg gtattaacta cagcgactat
     3661 ttttattctg tttctgcgac ttttcccaat gatggaaagt cttttcaaaa cttgatggag
     3721 aaggccgtta agaaggttga ggataagata atgctcgaaa aaataacttc cattgcggcg
     3781 gacaccatgg acaatgcaag aaaaacatac aaaaagtata aactataagg tagactatat
     3841 ctgtataaat aaattgggtt actaccttaa attaatgaat tggtggaaga a
//
LOCUS       DAOOJC010000232         3877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_18173_length_3877_cov_3.835950, whole genome shotgun sequence.
ACCESSION   DAOOJC010000232 DAOOJC010000000
VERSION     DAOOJC010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18173_length_3877_cov_3.835950"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..1440)
                     /locus_tag="PLA01_06970"
     CDS             complement(205..1440)
                     /locus_tag="PLA01_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HPD01078.1"
                     /translation="MLRNKRFFLALIMLILTLAGSAACLSINRNRSNRNITGEDIKNS
                     RIEFIQQNENKGSKEDSPPAVKPVKNDVKVKGVYVTGTSAGNKKYMEKLVNLINTTEL
                     NAVVIDVKEDGRVNYPSEIKTVKDIGAHQRLYDVDEVLKLLHDNGIYVIGRIVCFRDN
                     YLAQKRADLAVKRKDGSIWRENGSIAWTNPYNREVWKYNIDIAKEAVEKGFDEIQFDY
                     VRFPTVSDKEVNYGEKTPPKADAVSDFLKEAAVEINRLGVPVSADIFAIVCETPGDWE
                     GIGQVLERVGMDIDCISPMIYPSHYANASHGMMGNGVGQSINGVLFKAPDLKPYEVVY
                     NVLLKTKERISKVDGYRAKVRPYLQDFTASYLPKGYYQTYGPKQVRQQIKAVYEAGYE
                     EWILWDAANTYAESAFLKE"
     gene            complement(1670..3547)
                     /locus_tag="PLA01_06975"
     CDS             complement(1670..3547)
                     /locus_tag="PLA01_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPD01079.1"
                     /translation="MCKLKRVQLIALVLSIVLTQMFCFTMMADAAETATIDLSAEKQL
                     IQGFGGMNHPVWISDLTAEQRETAFGNGEGQLGFSILRIHVDENRNNWSKELATAKKA
                     IEKGAIVFASPWNPPSDMVETFTRNGVANQKRLRYDKYAAYAQYLNDFVAYMKNNGVD
                     LYAISIQNEPDYAHDWTWWTPQEILTFMKEYAGSINCRVMAPESFQYLKNISDPILND
                     PQALANLDILGAHFYGTSVNNMPYPLFEQKGAGKELWMTEVYVPNSDSNSADRWPEAL
                     DVAYNMHNALVEGNFQAYVWWYIRRSYGPMKEDGTISKRGYMMAHYSKFVRPGYVRVN
                     ATKNPTYNVYVSAYKKDNNVVAVVINKSTESKTINISVPGAKVQKWERYVTSSSKNLK
                     KESDISASGDTFQITLEPQSVTTFVGNAEDPVIPVERSAFSRLEVEEYDDIKSSTIQI
                     VGTAGGGKGLGYIENGDYTLYKNVDFKDGAESFTALVTTTANTSIELRLDSPTGTLAG
                     TLPVVSTGGFNAYEEQSCNINKVTGKHDLYLVFKGAVNIDWFTFGEGKTVGSKGDVNC
                     DGEIDSTDVTLLKRYLLRKASLTGESLSNADTNSDGEVDSTDLTLLKRYILRKIIVFP
                     A"
     gene            complement(3721..>3877)
                     /locus_tag="PLA01_06980"
     CDS             complement(3721..>3877)
                     /locus_tag="PLA01_06980"
                     /inference="COORDINATES: protein motif:HMM:NF017114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbfB domain-containing protein"
                     /protein_id="HPD01080.1"
                     /translation="SKNHPGYYLKHENFEIILKKYEDTDLFKDDATYKVVPGLADETR
                     ISFQSYN"
BASE COUNT         1088 a          883 c          617 g         1289 t
ORIGIN      
        1 attctttgat tattagatgt gccgactatt attaaccata gcagtacaaa aactcaagga
       61 caccaagcct tcggtttcca gctatttcgc aaaaaaacaa attccatacc tgatatagat
      121 taataatagc ataacatgtt tatatttata atactattta gacgagaata taacaataaa
      181 agttttattt tttttaaaag aaatttattc cttcaaaaaa gcggactccg catatgtgtt
      241 tgccgcatcc cacaaaatcc attcctcata tccggcttca tacactgcct ttatctgctg
      301 tctaacctgc ttaggaccat aggtctgata gtaacccttc ggcaaatatg aggctgtaaa
      361 gtcctgaagg tatggcctga cctttgccct gtaaccgtcc acctttgata ttctttcctt
      421 tgtttttaaa aggacattgt agacaacttc atagggtttt agatcaggag ctttgaacaa
      481 tacaccgttt atagattgtc cgactccatt gcccatcatg ccgtgtgaag cattggcata
      541 atgagacgga tatatcattg gagatatgca gtctatgtcc attcctacac gctctaaaac
      601 ctgtcctata ccctcccagt ctcccggagt ctcacataca attgcaaata tatccgccga
      661 aacaggtacc ccaagcctgt ttatttctac agctgcctcc tttagaaaat cggataccgc
      721 atcagctttg ggtggtgttt tttctccgta attaacctct ttatcgctca ccgtaggaaa
      781 ccttacataa tcgaactgta tttcatcaaa ccctttttcc accgcttcct ttgcaatatc
      841 aatgttgtat ttccacactt ccctgttata agggttcgtc caggcaatgc tgccgttctc
      901 cctccatatt gaaccgtcct tccgttttac ggccaaatcc gcccgctttt gagcaaggta
      961 attgtccctg aagcaaacaa ttcttccaat cacgtaaatc ccattatcat gcaatagctt
     1021 tagtacttca tccacatcat acaaccgttg atgggcacca atatccttta ctgtctttat
     1081 ctccgacgga taattaactc ttccgtcctc ttttacatcg attaccacag cattcaactc
     1141 tgttgtgttt ataagattca caagcttttc catatacttt ttgtttcctg ctgaagttcc
     1201 tgtaacataa acgcccttta ccttaacatc attttttaca ggttttactg caggcggact
     1261 atcttctttg ctgcctttat tttcattttg ttgtataaat tcaattcggc tgtttttaat
     1321 atcttcgccc gttatgttac gattgctgcg atttctgttg atgcttaaac atgcggccga
     1381 tcctgccaga gtcaatatta acataatcaa ggctaaaaaa aaccttttgt tccttagcat
     1441 ttctttcccc atttcactct gttataaaac atcactgcat cgttttactg caacctttta
     1501 tatattattt gctttgaaag attaaattta aacatgaaat ctttgactga tgggatataa
     1561 taccacaaaa cctccgaagc attatcgaat tagcattcgg aggttttgtt ctctatatgt
     1621 aataggagag ataataatta ttaatatatt ttatgatttt acaaacaatc tatgccggga
     1681 aaacaattat ttttctaagt atataccgct tcaacaatgt aaggtcggta gagtcaactt
     1741 caccatcact attagtatcg gcattggaaa gactttctcc cgtaagggac gcctttctaa
     1801 gtaaatacct tttcaataat gttacatccg tcgagtcaat ttctccgtca cagttaacat
     1861 cacctttgct gcccacagtt ttaccttcac cgaatgtaaa ccaatcaata tttacagcac
     1921 ctttaaatac aaggtacaag tcatgtttcc ctgtaacttt gttaatatta cagctttgct
     1981 cttcgtatgc attaaaaccg ccggttgata ccacgggtaa tgtgcctgca agagtaccgg
     2041 tcggactgtc caaccgcagc tcgatagatg tattggcagt tgttgtgaca agagcagtaa
     2101 aggattcggc accatctttg aaatcaacat ttttataaag tgtataatcg ccgttttcaa
     2161 tatatcccaa acctttgccg ccaccggcag tgcctactat ttgaatggta gaagacttaa
     2221 tatcgtcata ttcctcaact tccaaccttg aaaaagcact tctttcaacc ggtattaccg
     2281 gatcttcagc gttcccgaca aaggttgtaa cgctttgagg ctccaaagtt atttggaaag
     2341 tatctcctga tgcacttata tccgattctt tcttaagatt tttcgaactt gaagtaacat
     2401 acctttccca tttttgtact ttggcaccgg gaacagatat attaatcgtc ttagattccg
     2461 tacttttatt tatgacaaca gccacaacat tattatcctt cttgtaagcg gatacataca
     2521 cattgtacgt tggattcttt gtcgcattaa cccttacata tccgggacga acaaatttag
     2581 aataatgagc catcatataa ccccgcttac ttatagtacc gtcttctttc ataggaccat
     2641 aacttctgcg tatataccac catacatatg cctggaaatt cccctctacc aaagcattat
     2701 gcatattata ggcaacgtct aatgcttccg gccatcgatc cgccgaatta ctgtcactat
     2761 tcggaacata aacctcggtc atccagagct cttttcctgc tcccttttgt tcaaaaaggg
     2821 gatagggcat attattgaca ctggtaccgt aaaaatgggc accgagtata tccaaattgg
     2881 caagggcttg gggatcgttc aaaattgggt cggagatatt tttcaaatat tgaaatgact
     2941 ccggtgccat tactctgcaa ttgatagacc ctgcgtattc tttcatgaag gtaagtattt
     3001 cctgtggagt ccaccatgtc caatcatggg catagtccgg ttcgttctgg atagaaatag
     3061 catacaaatc cacaccatta ttcttcatat atgcaacaaa atcgttaaga tactgtgcat
     3121 aggctgcata cttatcgtac ctgagtcttt tttgattggc cacaccgttg cgggtgaagg
     3181 tttcaaccat atcactcggt ggattccatg gcgaggcaaa aactattgcc cctttttcaa
     3241 tggctttttt tgcagttgcc aattctttag accagttatt tctgttctcg tccacatgaa
     3301 ttctcaaaat agagaatccc aactgtccct ctccgttacc aaaagcggtt tctctttgtt
     3361 ctgctgtcaa atcactaatc caaaccggat gattcattcc tccaaaaccc tggattaatt
     3421 gtttttctgc cgacaagtca atggtagctg tttctgccgc atctgccatc attgtaaagc
     3481 agaacatctg agtcaagaca attgataata caagggctat aagttgcacc ctttttaact
     3541 tgcacatgtc aatcccccct acaattacta aaaaaggttg ttaattaaaa tcaattacat
     3601 aatttgtttg cattgctatt caacttcaat tttaatatat gtcgcatcct ctctgtcaag
     3661 atcggtttca atgttttcta tataaaggtt atattcccta tgcctaatat atctataagg
     3721 ttaattatag gactggaatg aaatcctagt ttcatccgcc aaacccggta caactttata
     3781 agttgcatcg tctttgaata aatccgtgtc ctcgtatttt ttaagaatta tttcaaagtt
     3841 ttcatgtttt aagtaataac caggatgatt cttcgat
//
LOCUS       DAOOJC010000233         3876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_18181_length_3876_cov_3.334467, whole genome shotgun sequence.
ACCESSION   DAOOJC010000233 DAOOJC010000000
VERSION     DAOOJC010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18181_length_3876_cov_3.334467"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLA01_06985"
     CDS             complement(<1..422)
                     /locus_tag="PLA01_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD01081.1"
                     /translation="MIEIQNLTKRYGQILAVDNLNFTVEKGEILGFLGPNGAGKSTTM
                     NIITGYLPSTEGTVKVSGFDIAQEPNEVKKRIGYLPENPPLYMDMTVDEYLNFVGQLK
                     KVERSERKKQISDIVELLKIADVRKRLIKNLSKGYKQRV"
     gene            complement(1154..>3876)
                     /locus_tag="PLA01_06990"
     CDS             complement(1154..>3876)
                     /locus_tag="PLA01_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HPD01082.1"
                     /translation="EENGVKVFASSKNQAQNIDVPGNIKQRIEAILANVDKWAGVLNE
                     NGEHVVDLRTPVPGPHFYTNLLLGNRVGYDHALQTTPKSVVDRLGRGSFRSHAATQVL
                     ATRWDMRQEENGFPANRQFYLVEDFKKIFYSADPNDANIESATCTHSQNHTVIQYRTK
                     CGLEIKRTIFILPQMEGMPLATEVQRIEIKNLTGKERNIKLVYTGMFGPATPHAIFED
                     VTYTNVIMQSSILRKEDGSIMAISPDYYPEPCKEDIRFSSMIIRNEDGIEFPKEFCAN
                     YNEFVGNGTLENPENLCKLSNLFYRKGPGFFALAGEIRLKPQGSAVADNFTGLSSNKV
                     NPDFNEDTYKLEIENLINRFSKAGEVEAALNTVKAFFDRYRGFMQINSKDPVFNTYVN
                     KNLPFQILYQTFVSRSFCQTQKGYREIGFREIQDIFAAMYYFVGMGEGDFVKQMLKEW
                     CSMIFEFGFAYHNFFWVGKEPGKWSDDALWFVQAAYRYINLTGDIGFLDEEVEIAGTS
                     PKSTRPVYDTIKALLKYSGEISVGKHGLPLLDNADWNDCLKLDKNFIDGITKEKLYRK
                     QLEEKGGKFGDPFESDYTESVMNAFLLKVAIDETISLCAKKGDMEYHDYLKKLSEKLY
                     GNLQEHAWKEDFFARTLFNRFKNNEFEFLGAKGDKLSADPEIDGSYFLNSFSWSVLAD
                     AASEEQISTMLDVIERTLKTPYGIKVVTLTDLGKLSDDTATGHYFPGDRENGGVFKHA
                     AMMGTAAMFKAAKQVKDKKLAARLANMGYWMVDLVAPYRTMQDPFSVCGNPRFCTQYN
                     NSETGENIGPMLSGTSTWMILTLMSAYGLEYTTKGIIIDPVIRENEKSSAYTVNTGKA
                     VYNISIKKPDGFCRIADGNAKITVDGSVIEGNLIPLFSDNKEHNIEVVFS"
BASE COUNT          994 a          929 c          705 g         1248 t
ORIGIN      
        1 accctctgct tataaccttt agaaaggttc ttaataagcc tttttctcac atcagcaatt
       61 ttcagcagtt ctacaatgtc cgatatctgc ttcttcctct cacttctctc cacttttttc
      121 aactggccta caaaattcag atattcatcc acggtcatat ccatgtaaag cggcggattt
      181 tccggcaaat agccgatgcg tttcttcact tcattcggtt cctgagctat gtcaaacccc
      241 gataccttta cagtcccttc agtcgaaggc aggtatccgg ttattatatt catagtagta
      301 gatttacctg ctccgttcgg acccagaaag ccaaggattt ctcccttttc aaccgtaaaa
      361 ttaaggttgt cgacagccaa tatttgaccg tatcgtttgg tcaaattctg tatttcaatc
      421 atctggtttc tcccctccta tttatttgtt gtcaaaaacg caagcacaat ttcattatcc
      481 taaattaatt ttaataaatt gtgaacaatt tgtaactaat cgatttattt ttttaagcgt
      541 aagccggtaa tttaatactt tatgcaagac caagagcggc gtgttaagaa aaagattgct
      601 ttgtaacatc tgatttcatt gctaatacag ctgattttac ttaatttttt agacttacat
      661 tatttatacc gacttgctaa ttatatagct tttttgacct cttttcaagt tgtatttaaa
      721 ttgccatata cgggcaaaac aaaaacagcc aatcggaatg ggctgtttga cttcggcatc
      781 cattaccaag aaaaaacagg cttaaaatgc ctgttcatca atttcctgct ttaaatttgt
      841 gatttcttct ttcgataatc ctgtaaattt tactacaagg tctatatcca tattctcttt
      901 tagcatatta atcgcaacct cttttttacc attctcaagc cctttctcaa ggcccttctc
      961 aaggcccttc cttgattcct ttttctaaag ctttttgatt catttcttca attgtattag
     1021 caagattgta aaccatattt tccacctcaa tgtaatacat agggagtttt gccctctgta
     1081 ataaaacaga cggcaaaact ccctgaaact tacaaagctt tatgcttaaa agcaccaata
     1141 ttaaattaca cccttaagaa aacacaactt ctatattatg ttctttgtta tcgctaaaca
     1201 gcgggataag atttccttct atcacacttc cgtcgacagt aatcttagca tttccgtcgg
     1261 caatacggca aaatccgtcg ggctttttaa tgctgatatt gtaaacggct ttaccggtgt
     1321 ttaccgtata tgcggaagat ttttcatttt ctcttatcac aggatcgatt attatcccct
     1381 ttgtggtata ttccaatccg tatgcggaca taagcgtcag tatcatccat gttgatgttc
     1441 cgctcagcat gggaccgata ttctctcccg tttcactgtt attgtactgg gtacagaacc
     1501 ttggatttcc gcagacagag aagggatcct gcattgttct gtacggggcc acaaggtcta
     1561 ccatccaata acccatattg gccagtcttg cggcaagttt cttgtcctta acctgctttg
     1621 ctgctttaaa catggctgcc gtacccatca ttgccgcatg tttgaacact cccccattct
     1681 cacggtctcc cgggaagtag tgtcctgtgg cagtatcatc ggaaagcttt cctaaatcgg
     1741 taagcgtaac aaccttgata ccgtaagggg tcttgagtgt cctttcaatt acgtccagca
     1801 tagtcgatat ctgctcctca cttgcagcat cggcaagcac cgaccaactg aaggaattca
     1861 agaagtatga gccgtcaatc tccggatctg cggaaagttt gtcccctttt gcccctaaaa
     1921 attcgaactc attgttttta aacctgttga aaagtgtcct tgcaaagaaa tcttccttcc
     1981 aagcgtgctc ctgaagattt ccatacagct tttccgacag cttcttgagg taatcatggt
     2041 attccatgtc tccctttttg gcacaaaggc taatggtctc atctattgca acctttaata
     2101 ggaaggcatt cattacactt tcggtataat cgctttcaaa aggatctccg aattttccgc
     2161 ccttttcttc aagctgtttg cggtaaagct tctcctttgt aataccgtca ataaagttct
     2221 tgtcaagctt caagcagtca ttccagtccg cattgtccaa tagaggaagc ccgtgttttc
     2281 caacggatat ctctccggaa tatttcagca atgcctttat tgtatcgtat acaggtcttg
     2341 tactctttgg agaagtaccg gcaatctcca cctcttcatc caaaaatcct atatccccgg
     2401 taaggtttat atagcggtat gcagcctgta caaaccacaa agcgtcatcc gaccatttgc
     2461 ccggctcttt tcctacccag aagaagttgt ggtatgcaaa accgaactca aatatcattg
     2521 aacaccattc tttgagcatc tgctttacaa aatcgccttc tcccatgccg acaaaatagt
     2581 acatggctgc aaatatatcc tgaatctccc tgaatccgat ctccctgtag cccttttggg
     2641 tctggcagaa ggaccgggac acaaaggtct gatacagtat ctggaacgga agatttttgt
     2701 tgacataggt attgaatacg ggatctttgc tgtttatctg catgaaaccc ctgtacctgt
     2761 cgaaaaacgc cttaacggtg tttaatgctg cctcaacctc tccggccttt gagaaccggt
     2821 taatgagatt ttctatttcc agcttgtagg tatcctcatt gaaatcggga ttaaccttgt
     2881 tggaagaaag accggtaaag ttgtcagcca cagcactgcc ctggggcttt agcctgatct
     2941 cccctgccag ggcaaagaaa cccgggcctt tgcggtaaaa aaggttactg agcttgcata
     3001 gattctcagg attttcaagg gttccgttcc ctacaaattc gttatagttt gcacaaaact
     3061 ccttgggaaa ctcaataccg tcttcatttc ttataatcat tgaactgaac ctgatgtcct
     3121 ctttgcatgg ctcgggataa taatccgggc ttatcgccat gattgagccg tcctcttttc
     3181 ttaggatgga tgactgcata attacattgg tataggttac atcctcaaaa atagcatgag
     3241 gtgttgcagg cccgaacata ccggtgtaaa ccagcttaat gttcctttct ttgccggtca
     3301 ggttctttat ttctatcctt tgaacctcag tagccaaagg cattccttcc atctggggaa
     3361 ggataaaaat agtccttttt atctccagtc cgcacttggt cctatactgt attacagtat
     3421 ggttttgtga gtgggtacat gttgcggact ctatattggc atcgttgggg tccgcagaat
     3481 agaatatttt tttgaagtcc tccaccagat aaaactggcg gtttgcaggg aaaccgttct
     3541 cttcctgtct catgtcccaa cgagtcgcca aaacctgggt tgcggcatgg gaacggaagc
     3601 ttccccttcc cagcctgtcc acaacgctct tgggcgtggt ttggagtgca tggtcatatc
     3661 ccaccctgtt gcccaaaagc aaatttgtat aaaagtgagg tcccggtacc ggtgtacgaa
     3721 ggtctacaac atgctctccg ttttcgttca atacaccggc ccatttatcc acattagcca
     3781 ggatagcttc aattctctgc tttatattcc cgggcacatc tatgttctga gcttgatttt
     3841 tgctgcttgc aaaaaccttt acaccatttt cttcca
//
LOCUS       DAOOJC010000234         3845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_18370_length_3845_cov_2.904222, whole genome shotgun sequence.
ACCESSION   DAOOJC010000234 DAOOJC010000000
VERSION     DAOOJC010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18370_length_3845_cov_2.904222"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PLA01_06995"
     CDS             complement(<1..452)
                     /locus_tag="PLA01_06995"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HPD01083.1"
                     /translation="MEIKSLKKLKLYGFNNLTKTLSFNIYDICYAKDIEHRKGYIEYI
                     DEQYNAKRLTKILSNVSAIIGANILNISSQDYEPQGASVTMLICEEKINNGHMDSSCD
                     AETPGPLPETVLAHLDKSHITVHTYPESHPDDGISTFRADIDVSTCGR"
     gene            690..2234
                     /locus_tag="PLA01_07000"
     CDS             690..2234
                     /locus_tag="PLA01_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPD01084.1"
                     /translation="MPKNGFDIMAAIDVGSNSLRMIIAQVTSDGQVIPLEDLYKPTQI
                     GKDTFSFGRIQVQSIHDTCETLKGFNRLMQDYKLKNYRAVSTSGIREAQNREYVLEQI
                     RIKSGLEVEVINNAQERFLMYKAIRNYMSDEKRFAGKGTMIADIRSGGVEISVYSEGS
                     LKFTEYIKVGSLRLREILADLEKRTLDFPSLMEEFIDSRIDSLKSILPEFKIKNFVGL
                     GGELKTIINLCIDKSDGGRNYIKKDELKKLYSKVHSLTTSQIIDEYGLSRNQAEILLP
                     SVILFNSFLNMTDAEGIYAPMVSLRHGLLAHMADERFNTAGRKEANNDIVSSVWFIGK
                     KYSIDKVHSGYIEKLSLSIFDQMKKIHMLGESERLYLRVAAILHDIGKYVNHNNHDVH
                     SYNIIRFQDIMGFSDRELSLVANIARYHSDEIPNLSHDNYSMLSSRDQIIVSKLAVIL
                     RIAEAMDISHKQKIEKVEIHKHGKELEFILWSKEDILLEEWSFNSNSNFFEEVMGNKP
                     VLKHKG"
     gene            2241..>3845
                     /gene="ppk1"
                     /locus_tag="PLA01_07005"
     CDS             2241..>3845
                     /gene="ppk1"
                     /locus_tag="PLA01_07005"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPD01085.1"
                     /translation="MKKLEYDLKRNYFNRELSWLEFNERVLGEACNSNQNPIFERVKF
                     LSIVSSNLDEFFMVRVASLFDQMLAGFNEPDIAGFTPREQINLISTRAYRMISDQYNC
                     YNRSIIPGLKKAKITFLKPGDLNDKQKYFVDDFYKNTVYPVLTPMVVDRSRPFPLVLN
                     KSLNIALLLENKEDEEDPIFAMVQVPSVLERLVELPSNEGKKCFILLEDIIKMYIDTL
                     FTGHDIITMACYRVTRNTDQGLDEEEAEDLLEAIQQYIKKRKWGMVIRLEIEHGMEPD
                     LLEILIDELEAPRETVYEIKGPLDLTFLMRIHSLPEYDHLKYPIHRQQPVPEFWGRED
                     IFEAISERDILIHHPYHSFEPVMEFVRQAANDPKVLAIKQTLYRVSGHSPIVEALAQA
                     AENGKQVTVLVELKARFDEQNNIIWAKRLEMAGCHVIYGLVGLKTHCKILLVVRMEED
                     GIKRYVHLSTGNYNDITARFYTDIGLFTSNPYFGADASALFNMLSGYSRLNRMYKLDV
                     APMTLRDKFKRLIRQEAENARRRKKAR"
BASE COUNT         1200 a          651 c          857 g         1137 t
ORIGIN      
        1 atgcgtccac aggttgagac gtctatatct gctctgaagg tgctgatacc gtcatcaggg
       61 tggctttcag gataagtgtg tactgttatg tggctcttat ctaagtgtgc aagtacggtc
      121 tcaggaagag gccccggtgt ttctgcatcg catgaagaat ccatatgccc attgtttatt
      181 ttttcttcac aaataagcat ggtaacactt gcaccttgag gctcataatc ctgactcgag
      241 atattcagta tatttgcacc gattatcgca gatacattgg acaatatttt tgtaagcctt
      301 tttgcattat actgttcatc tatatattct atatatcctt tgcggtgttc gatgtctttt
      361 gcatagcaga tatcatatat attaaagctt aatgtttttg ttaggttgtt aaaaccgtac
      421 aatttaagtt tttttaatga tttaatctcc atgatcttct ccttgttctg acctataatt
      481 tctatacttt ttaccctact attataattt cattatgata ttttgtaaat ggtacacaga
      541 aaatatgtag attcacaatt tccctgcaca tttttcttaa cttacataat atacattaca
      601 gtaaatagct gaatatttac gataaagatt taaaatggta taatattcac tatatagaat
      661 atagcttttc ggaaagggtg tagtcttata tgccaaagaa cgggttcgat ataatggctg
      721 caattgatgt aggctcaaat tcgcttagaa tgattatagc acaggttaca tccgacggac
      781 aggtgatccc tcttgaggat ttatataagc ctacacaaat aggcaaggac accttttctt
      841 ttggaagaat tcaggttcaa tcaattcatg atacctgtga aacgcttaag ggttttaaca
      901 ggctgatgca ggactataag cttaaaaatt acagggctgt gagtaccagt ggtattaggg
      961 aagcccaaaa cagggaatat gtgctcgagc agatcaggat aaaatccggg cttgaggttg
     1021 aagtgattaa caatgcccag gaaaggtttt tgatgtataa ggccatccgc aattatatgt
     1081 ccgatgaaaa gaggtttgcc ggaaaaggaa ccatgattgc ggatattcgt tcgggtggag
     1141 tggagatatc ggtatacagt gaaggaagcc tcaaatttac ggagtatatc aaagtagggt
     1201 ctttaagact tcgtgagatt ctggcggatc tggaaaagag gactcttgac tttccaagtc
     1261 ttatggaaga atttattgac agcagaattg attcattaaa gtcaattttg cctgagttta
     1321 agattaaaaa ctttgttgga ctcggcgggg aattaaaaac cataataaat ttatgcatcg
     1381 ataaatcgga tggcggcaga aattacataa aaaaggatga gcttaagaag ctgtattcca
     1441 aggtgcatag tcttacaacc tcacaaataa ttgatgaata cggtcttagc aggaatcagg
     1501 ctgaaattct tttgccgtcg gtcatccttt ttaatagttt tctcaatatg acagatgctg
     1561 aaggaatata tgccccgatg gtttcgttaa ggcacggact tttagcacat atggcagatg
     1621 aaaggtttaa tactgccgga agaaaagagg ccaataacga tatagtcagt tctgtttggt
     1681 ttatcgggaa aaagtattcc attgacaagg ttcattccgg ttatattgag aagctttccc
     1741 tgtcgatttt cgaccagatg aaaaaaattc atatgctggg tgaaagtgag aggctgtacc
     1801 ttcgagtggc ggcaatactc catgacattg gaaagtacgt taatcacaat aatcatgatg
     1861 ttcactcata taacataatt cgtttccagg atataatggg tttttcagac cgtgaactaa
     1921 gtcttgtggc caatattgca aggtaccaca gtgacgaaat tccgaacctt tctcatgata
     1981 attattcaat gctaagctcc cgggatcaga taattgtatc caaactggcg gttattttaa
     2041 gaatagctga ggctatggat atatcccata aacaaaaaat agagaaggtt gagatacaca
     2101 agcacggaaa agagcttgaa tttattctat ggtcaaaaga ggatatcttg ttggaagaat
     2161 ggagttttaa cagcaattcc aatttttttg aagaagtaat gggaaataag cctgtattaa
     2221 agcacaaagg atgattcaag atgaagaaac tggagtatga cttgaaaaga aattatttta
     2281 accgtgaact aagttggctt gagtttaatg agagggttct gggtgaagcc tgtaacagca
     2341 atcagaatcc catatttgaa agggttaagt tcctttcgat agtgagctca aaccttgatg
     2401 agttttttat ggtaagagtg gcatctttgt tcgatcagat gcttgcaggt tttaacgagc
     2461 ctgatattgc aggttttacg ccccgggaac agattaattt gatttcaaca agggcataca
     2521 gaatgatcag tgaccagtac aattgttata accgttcaat tatacccggg cttaagaagg
     2581 caaaaataac ttttttaaag cctggggatc ttaatgacaa acagaagtat tttgttgatg
     2641 atttttataa aaatacagtt tatccggtac ttactcccat ggtggttgac agaagcaggc
     2701 cttttcccct tgtactcaac aaaagtctta atattgcact gcttcttgaa aacaaagagg
     2761 atgaagagga tcccatattt gctatggtac aggtaccctc tgtacttgaa aggctggttg
     2821 aacttccttc aaatgaagga aaaaagtgct ttattctgct tgaggacatt ataaaaatgt
     2881 atattgatac actttttacg gggcatgata taataaccat ggcttgttac cgcgtcacaa
     2941 ggaatacgga ccaagggctt gacgaagaag aggcggaaga ccttcttgaa gcaattcagc
     3001 agtatattaa gaagagaaaa tggggaatgg tgataaggct ggaaattgag cacggtatgg
     3061 aaccggacct gttggagatt ctgattgatg agttggaggc accacgggaa accgtatacg
     3121 aaataaaagg acctttggat ttgacttttc ttatgagaat tcattccctc ccggagtatg
     3181 atcatctaaa atacccgatt caccgtcagc agcccgtacc ggagttctgg ggcagggagg
     3241 atatttttga agcgatctcg gaaagggaca tccttattca tcacccttat cattctttcg
     3301 agccagttat ggaatttgtg agacaggctg ccaacgaccc caaggtactg gcaattaaac
     3361 agacacttta cagggtcagc ggccattctc cgatagtaga agcattggca caggcggcag
     3421 agaacggaaa acaggttacg gtgcttgttg agttgaaagc acggtttgat gaacaaaaca
     3481 atattatctg ggcaaagcgt ctggaaatgg caggatgcca tgtaatatac ggactggtcg
     3541 gacttaaaac ccattgcaag atacttcttg ttgtgaggat ggaggaggac ggcattaaaa
     3601 gatatgtgca tttaagtacc ggaaattata acgatataac cgcaaggttt tataccgata
     3661 ttgggctttt cacctcaaat ccgtatttcg gagccgatgc atcggctttg ttcaatatgc
     3721 tttccggtta ttcgcggcta aacaggatgt acaagctgga tgttgcaccc atgaccttaa
     3781 gggataaatt taaacggctt ataagacagg aagctgaaaa tgcaaggaga agaaaaaagg
     3841 caagg
//
LOCUS       DAOOJC010000235         3806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_18613_length_3806_cov_3.015996, whole genome shotgun sequence.
ACCESSION   DAOOJC010000235 DAOOJC010000000
VERSION     DAOOJC010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18613_length_3806_cov_3.015996"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3806)
                     /locus_tag="PLA01_07010"
     CDS             complement(<1..>3806)
                     /locus_tag="PLA01_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HPD01086.1"
                     /translation="KKLISNELYEFGGKNALINLENGGGKSVILQLALQVVLPNTNMG
                     SRNFSHYFKTGTSPTHIMVEWKLDGTGADYVLTGICVSGGTDGLRFFTYTHFYKLPHD
                     LDIKGLEAINREKQVIGFSEYYNYLKRLANERRLNLNIYSRDRQREYRDKLYTFNLFR
                     EEFDAIKIINQTEGGIDKFFENARKSRNVIEKLIIPNIPQAEGETSGLLVQTLKKHLD
                     NLKNIPIYQHKIKTYEAFCDRAEELLSKLNDYGTKVEEIDSVSRDILALSNIIGIAAE
                     RLEKEVESLKNNEQEYAARIEELLYKKDSLDYTKRVSEMENLEERHEQVKREIEKISD
                     KIQELDREIRYKEAASFYDDIVKARNEVLLYREQLESLSKEKDEIHREYQNYIYHAKI
                     LLGNEIKGISEAIEDLKRDQNSLLKEKKGLQEKLDAKNKERDEVKHNISAFETKLEHN
                     KERQSQIEGYFVKDVTLLADPRDGLEKIVEERDGLLNSNNNLKEEIDKANRAIDDLAI
                     NENRLKEALAASRQKKESIEEEIKDFERKFAKINKEISMYEIDDDVYSNKTMESLNAL
                     KLKMDNSLRDVLFRYREFLNRKNLHKDSEYYIPDAEIKKVYDLLVERGIRCIPGSLWL
                     RNQREDLKEAFLHKNPLLSYSIVIEKRELEKIMNGEGTLLKEVRDLVESYPVTFVVDC
                     EQGILTLDNEDMPKSGIDRLGAMEAYVVYSKNNSLALNTELFKQYLKDIDQRILGLDS
                     ECEGIKKDVEKITGLIERCREFVSSYPKGYIKELKGRRDALLDDIKNHEDNIQSIQER
                     RDFIHKSKIENQKIIEANEGQIKEKEQDIDNLKEYMKLRAKISELAGHCKTEQQRLNK
                     TEKEKKEIEERIDSVSKEIEDARYNLREYSRTSDEKKKRFDEISARLTITEPVMHITG
                     TLNEILSKARGLETSMDDSGIDQVRNLIENWVETEKRGLNQIRSKGFTEADFEVKVIH
                     FNEEELEEERKQLDSLKKDKKGLDENERRLFGEIKGLGGKTEQLEKDILNKYKNPPCI
                     FDSLESVDETAFEKQIDAFRRKRQNNTKKLEDTDRRRNKMVEHRNRLEEDIEEYGIKI
                     QDSSRENMDSLVYNGETVSLWDILRLPLENIVVIGNEKRRRYKAITKNLDEMESRIKE
                     SYDKLYNDADWAENGTIRLILEKIMKNDMYNYKYVEELFNDILKSVENMKKATQFQLD
                     ESLKDKDEIVERCYSKAERVYEEIKSVDGFSKIKVDGVNRKTINIDMPALHREEGRAL
                     MNRYIE"
BASE COUNT          887 a          922 c          570 g         1427 t
ORIGIN      
        1 ttcaatatac cggttcataa gggctctgcc ctcttcccgg tgtagggcgg gcatatcaat
       61 atttatggtt tttctgttaa caccatccac cttgattttg gagaaaccgt ccactgattt
      121 tatctcttca tatacccttt cagccttact gtagcaacgt tccacaattt catccttgtc
      181 ttttaaagac tcatccaatt gaaattgcgt tgcctttttc atgttttcca cgctctttag
      241 tatgtcatta aacagttcct ccacatactt atagttatac atatcatttt tcattatttt
      301 ttccagtata agccttatcg tgccgttttc cgcccaatcg gcatcattgt acagcttatc
      361 atagctctct tttatacgag actccatttc atcaaggttt ttggttatag ccttgtaacg
      421 ccttcttttt tcatttccta taaccacaat gttttctaac ggaagcctta atatatccca
      481 caatgaaacg gtttccccgt tataaacaag gctgtccatg ttctcccttg aggagtcctg
      541 gatttttatt ccgtattcct caatatcctc ttccagcctg tttctatgct ccaccatctt
      601 gtttcttctt ctatccgtat cctcaagttt ttttgtatta ttctgcctct ttcttctaaa
      661 cgcatcaatt tgtttttcaa aggcggtttc gtccacactt tccaacgagt cgaaaatgca
      721 gggaggattt ttatatttat ttaaaatgtc tttttccagt tgttctgtct ttccccccaa
      781 ccccttgatt tctccaaaca accggcgttc attttcatcc aggccttttt tatctttctt
      841 taaagaatca agctgttttc tttcttcttc aagttcttcc tcattaaaat gaatcacttt
      901 cacctcgaaa tcagcttcgg taaaaccttt ggatcggatt tgattaagac ctcttttctc
      961 agtttcaacc caattttcaa tcagatttct tacttgatca ataccgctgt catccatact
     1021 ggtttcaagc ccgcgggctt tagagagaat ttcatttaaa gttccggtta tatgcattac
     1081 cggctctgtt atggttaacc gggcggaaat ctcatcaaaa cgcttcttct tctcatcgct
     1141 tgtgcgggag tattccctca aattatatcg ggcatcttct atttcctttg atacagagtc
     1201 tattctctcc tcgatctctt ttttctcctt ctcggtttta ttcagtcttt gctgctccgt
     1261 cttacagtgg ccggcaagtt ctgatatttt tgccctgagc ttcatatatt ctttgaggtt
     1321 atcaatatcc tgttccttct cttttatttg cccttcgtta gcttcaataa ttttctggtt
     1381 ctcaattttg cttttatgta taaagtccct tctttcctgt atgctttgga tattgtcctc
     1441 atgattttta atatcatcta aaagggcatc ccggcggccc tttaactctt ttatatagcc
     1501 tttaggatac gagctcacaa attcccggca cctctcaata agcccggtta ttttctccac
     1561 atcttttttt ataccttcgc actcgctatc taatcctaga atcctctgat caatatcctt
     1621 aagatattgt ttgaaaagtt cggtatttaa ggcaagggaa ttgtttttag aatacactac
     1681 ataggcttcc attgctccta acctgtcaat gccgcttttt ggcatatcct cattgtccaa
     1741 agtcaaaatt ccctgttcac aatccacaac aaaagtaacg ggataacttt ccaccagatc
     1801 ccttacctct tttaacaagg tgccctcccc gttcattatc ttttccagtt ctcttttctc
     1861 tatcacaatg gaataactca aaaggggatt tttgtgcaaa aaagcttctt ttaaatcttc
     1921 cctctggttc ctaagccata acgagcccgg tatacacctt ataccccttt ccaccagcaa
     1981 atcataaacc tttttaattt ctgcgtccgg aatgtaatac tcactgtctt tgtgcaggtt
     2041 tttgcggttt aagaactccc ggtatctaaa aagaacatcc cttaaggagt tatccatttt
     2101 tagtttcagt gcattcaaag actccatggt tttattgctg tagacgtcat catcaatctc
     2161 atacatggat atttccttat ttatttttgc aaacttcctt tcaaaatcct taatctcttc
     2221 ctctatgctt tccttctttt gccgggaagc tgcaagggct tcttttaaac ggttttcatt
     2281 tattgccaga tcatcaattg ccctgtttgc cttgtctatc tcttctttga gattattgtt
     2341 gctgtttaaa agcccatccc tctcctccac tatcttttca agtccgtccc tgggatctgc
     2401 caaaagggtg acgtctttta cgaaataccc ctctatctgc gattgtctct ctttgttatg
     2461 ctcaagtttt gtctcgaagg ctgaaatgtt gtgcttaacc tcatcccttt ctttattctt
     2521 tgcatcaagt ttctcctgaa gccccttttt ttccttcaac agactgtttt gatccctttt
     2581 caaatcctct atggcctctg aaatcccctt tatttcattc ccaagcaaaa tttttgcatg
     2641 atatatatag ttctggtact ccctgtgaat ttcatccttt tccttggaaa gagattccag
     2701 ctgttctcta tataacaaaa cctcatttct tgcctttaca atatcatcat aaaaagatgc
     2761 ggcttcttta tatctaatct cgcggtcaag ttcctgaatt ttgtcggata tcttttcaat
     2821 ttcccttttg acctgttcat gcctctcttc aaggttttcc atctccgaaa ccctttttgt
     2881 gtaatcaagg ctgtcctttt tatacaacaa ctcctctatc cgtgccgcat attcctgttc
     2941 attgttcttt aagctttcta cttccttttc aagcctctcg gctgcaatac cgataatatt
     3001 tgaaagtgcc agtatatccc tgctgacact gtctatctct tctacctttg taccgtaatc
     3061 attgagcttg gaaaggagtt cttcagccct atcgcaaaag gcctcatagg tctttatttt
     3121 atgttgataa atggggatgt tctttaagtt gtctaggtgc tttttcaatg tctgaaccaa
     3181 caacccggaa gtttcaccct ctgcctgggg tatattgggt ataataagct tttcgattac
     3241 atttctggac tttcttgcat tttcaaagaa tttatctata ccgccctcgg tttggtttat
     3301 tattttaatg gcatcaaact cttccctgaa gaggttaaag gtataaagct tgtccctgta
     3361 ttccctctgc cggtctctag aataaatatt taaattaaga cgcctttcgt tggcaagcct
     3421 ctttaaataa ttatagtact ctgaaaagcc gataacctgt ttttctctgt taatcgcttc
     3481 aagacctttt atatccaaat catggggaag tttatagaaa tgagtgtagg taaaaaacct
     3541 tagtccatcg gtccctcccg aaacacagat gcctgtcagg acatagtcag ctcctgtgcc
     3601 atccagcttc cactccacca taatatgtgt gggtgacgtt ccggtcttaa aataatggga
     3661 aaaatttctg cttcccatat ttgtgttggg aagcactacc tgaagtgcca attgaagaat
     3721 gacactcttt ccacccccgt tttccagatt gattagggcg tttttaccgc caaactcata
     3781 aagctcattg gatataagct ttttgt
//
LOCUS       DAOOJC010000236         3804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_18628_length_3804_cov_3.399573, whole genome shotgun sequence.
ACCESSION   DAOOJC010000236 DAOOJC010000000
VERSION     DAOOJC010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18628_length_3804_cov_3.399573"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..505
                     /locus_tag="PLA01_07015"
     CDS             125..505
                     /locus_tag="PLA01_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HPD01087.1"
                     /translation="MLKKGDFFIILIVIIMTVVSFVGVLIYKGWEEDGRKVALIIRDN
                     EEIKRIDLDAVDEPWEYTVSGDYSNTILVERGRVRFKEADCPDEVCVDTGWLYKKGDM
                     AVCLPNRIMIKIEGEPEDVDIVTY"
     assembly_gap    548..557
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <558..1040
                     /locus_tag="PLA01_07020"
     CDS             <558..1040
                     /locus_tag="PLA01_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HPD01088.1"
                     /translation="LGLLISQALVLSIIESWIQIPVPVPGVKLGLANIITVVVIVYFG
                     FREALTVVIVRCVLTSFFGGGGLMFFFFSAAGGILSTMVMSVLYKAGRNKFSLTGISI
                     AGAITHNIGQLLVAAFFMRDPAVAVYLPALLVSGCIMGFFVGFVSNLLVNALNKTKIF
                     "
     gene            1202..1615
                     /locus_tag="PLA01_07025"
     CDS             1202..1615
                     /locus_tag="PLA01_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01089.1"
                     /translation="MIKVVCGKKGVGKTRALVNSANSFLSSASGDIVFISGKRQLMHD
                     LSHKIRLINLSEFPVNISSSCTFLGFICGIISANYDTKGIYIDDLVSIVKNVPEGLKE
                     MFDGMNEVSEKFNIDFHVSIEGDPDSMPDYVKECY"
     gene            2085..>3804
                     /gene="priA"
                     /locus_tag="PLA01_07030"
     CDS             2085..>3804
                     /gene="priA"
                     /locus_tag="PLA01_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPD01090.1"
                     /translation="MKNIASVAIKNSTRKFDREYHYIIPDEYIGKLVPGFRVIVPFGK
                     SNRLVEAYVLDTCEKADVIELKEIDRVLDEKPVLNNNMIRLAQWMKERYICTYSDAIR
                     CMIPPGTGVNSTKIIKLIKNEGSLKGTKKKIMDCLCEHEGEMDYEELKSLTKAKVLSK
                     HLESLAQEGYIEVIEQFTARVREKQIKAVYLIKPEDEVIYDIESNRIKNIKQIRVLEM
                     LIENEYIAVNDIINFAGIYRGGLETLKKHGYIDFMDVEVTRDPVGDRNYKKTHPLDLL
                     PEQRNAYDRVTGMLDSGSFNEVLLHGVTGSGKTEVYLQLIDHCINTGKQAVVLVPEIS
                     LTPQMVERFKGRFGDDVAVMHSRLSLGERYDQWRLIRDGKTRVVVGARSAVFAPFENL
                     GLIIIDEEHENSYKSEIVPKYHAADVARQRCLLENAVLLYGSATPSVETYYRAKTGEI
                     ELLEMSKRANNMLLPKVEVIDMRAEMNNGNRSVFSERLKAEIKVNIEKGQQTIVFLNR
                     RGYASFVLCRSCGYVLKCPYCNVSLTYHSHEDRVICHYCGFTLKNPSACPKCKSNHIR
                     NFGTGTQ"
BASE COUNT         1205 a          583 c          939 g         1067 t
ORIGIN      
        1 gtgaggctta ttgaggggat tgacgggaca gaggcggttt tcatttcgaa ggataataaa
       61 gtctatataa cggacggatt aataggcatt tttgaattta aggatgaaag taaggaattt
      121 gaatatgttg aaaaaaggtg acttttttat tattttaatt gttataataa tgacagtggt
      181 ttcttttgtc ggtgtcttaa tttataaggg atgggaagag gacggccgca aagtggcatt
      241 aataatccgg gacaatgaag agataaaaag gattgatcta gatgccgttg atgaaccatg
      301 ggagtataca gttagtgggg attacagcaa tacaatactt gtggaaagag gtcgggtaag
      361 gtttaaggaa gctgactgtc ccgatgaggt gtgtgtagat accggatggc tttataaaaa
      421 gggagacatg gcagtgtgcc ttcccaaccg aattatgata aaaattgaag gagaaccgga
      481 agacgttgac atagtaacat attagaaaaa acataaaaaa agaatggggt agaaagtgag
      541 tagtgtannn nnnnnnnctt gggttgttga tttcgcaggc attagtttta tcgataattg
      601 aatcatggat acagatacct gtgcctgtac ctggagtaaa gcttggtctt gccaatatta
      661 taaccgtcgt ggttattgtt tatttcggat tcagagaggc tttgacagta gttattgtaa
      721 ggtgcgtttt gacctccttt ttcggcggcg gaggcctgat gttctttttc ttcagtgctg
      781 ccggggggat attaagtacc atggttatgt cggtcctata taaagcagga cgcaacaaat
      841 tcagtctaac aggtataagc attgccgggg caataaccca taatatcggg caattgctgg
      901 tggcggcttt ttttatgcgg gatccggctg tggcggttta tcttcccgcg cttctcgttt
      961 caggatgtat aatgggtttc ttcgtaggct ttgtcagcaa tctattggta aatgcattga
     1021 ataagacaaa gatattttga cataaaaatc aagacaaaga aaaaccattt taatatgctt
     1081 atttctagaa aaagcaagtt attaattgta tgtaaaatat taataaaata ggcttgaaaa
     1141 cattggtgat ttatattatc gtataattat agagtaaaat aaaaagaagg gatagatgct
     1201 gatgattaag gtagtttgtg gtaaaaaagg cgtaggaaag acaagggctc ttgtaaattc
     1261 tgcgaacagt tttctttctt cagcttcggg cgacatagtt ttcataagtg gaaaaagaca
     1321 gttaatgcac gacttaagcc ataaaattag attgattaac ttatctgaat ttccggtcaa
     1381 catcagcagt tcctgtactt ttctcggttt tatctgcgga ataatctctg ccaattatga
     1441 tactaagggg atatatattg acgatcttgt cagcattgtc aagaatgttc ctgagggctt
     1501 gaaggagatg tttgacggta tgaatgaagt atcagaaaaa tttaatatcg atttccatgt
     1561 ttctattgag ggggatcccg attccatgcc ggattatgtg aaggagtgtt attaaaggct
     1621 ggaagaggga tttgtttttt accactaaaa atgataaaac cgaaaataaa ttaaagctta
     1681 aggggcaggg gaacccttat gctaatacag gattaaagag tacttattaa aggccggata
     1741 aaacaacgaa cttatccggc ttttttggtg ggatttcaca aacagaggtg tatattgctt
     1801 aagtgaagaa aaatgtggaa gaaacattgt gaagcaagtt gtagagcatg ttaatacgac
     1861 acggaaaggg agagcattgt ccgaagcgaa gcaagttttg ctcgaccccg cggagtattt
     1921 acaggctcta gacttgcgaa gcttaagttc ctggaacatt tttcttcacg tttatttaac
     1981 acaccaacga aacagttttt gttgctttat gagccgtgct gttgctataa tatattaata
     2041 ccctatattt cgagggcaag tataatatat aaaaaggtgc agggatgaaa aacattgctt
     2101 ctgttgcaat aaaaaactca acaaggaaat tcgataggga ataccactat ataatccccg
     2161 atgagtacat tggtaaattg gttccggggt ttagggttat tgtgcctttt ggaaagtcaa
     2221 acaggcttgt tgaggcatat gttctcgata cgtgcgagaa ggcggatgtt atagagctca
     2281 aagagattga cagggtgctg gatgaaaagc ctgttcttaa caataatatg ataagactgg
     2341 cccagtggat gaaagaaagg tatatctgta cctattccga tgcaataagg tgcatgattc
     2401 cgccgggaac aggcgtaaat agcaccaaaa taattaagct gataaaaaac gagggttctc
     2461 tcaagggaac taaaaaaaag attatggact gtttgtgtga gcatgaaggt gaaatggatt
     2521 atgaggaact taagagcttg acaaaagcaa aggttttgtc caagcatctt gaaagtctcg
     2581 cacaagaggg atatatagag gttatagagc agtttaccgc ccgggtcagg gagaagcaaa
     2641 taaaggcagt gtaccttata aaaccagaag atgaagtgat ttatgacata gaaagtaaca
     2701 gaattaaaaa cataaagcaa atccgggttc ttgagatgct tatagaaaat gaatatatag
     2761 cggtcaatga cataataaat tttgccggaa tttaccgtgg gggattggaa acccttaaaa
     2821 aacacgggta tattgatttt atggacgtag aagtaaccag ggatcctgta ggagacagaa
     2881 actacaaaaa aacccatcca ttagatctat tgccggagca gagaaatgct tatgaccggg
     2941 tgacagggat gctggactcg ggaagcttta atgaggtatt gctccatggt gttacaggaa
     3001 gcggaaaaac cgaggtctat cttcaattga tagaccattg tataaatacg ggtaagcagg
     3061 ctgttgtgct ggttccggag atatctctga ctccccagat ggttgaaagg tttaagggca
     3121 ggtttggaga tgatgtagcc gtaatgcaca gccggctttc tttaggggaa aggtacgacc
     3181 agtggaggct tatcagggat ggaaagacca gagttgtggt aggtgccagg tctgccgtgt
     3241 ttgcaccttt tgaaaacctt ggacttataa taattgacga agaacatgaa aactcgtata
     3301 aatcggaaat tgtgcccaaa taccatgccg ccgatgttgc aagacagcgt tgcctattag
     3361 aaaatgctgt gctcttgtac ggttcggcca ctccttcagt ggaaacatac tatcgggcta
     3421 aaacaggaga aatcgagctt cttgaaatgt caaaaagggc caacaatatg cttctgccca
     3481 aggtagaggt tattgatatg cgggcggaga tgaataacgg caaccggtcg gtgttcagcg
     3541 agaggctcaa ggccgagatt aaggtaaata ttgaaaaagg acagcagacc attgtttttt
     3601 tgaacaggag ggggtacgca tcctttgtat tgtgcagaag ctgcggctat gttctaaaat
     3661 gtccttattg taatgtttct ttgacttatc attcccatga ggacagggtt atctgccact
     3721 attgcggttt tactctgaaa aacccttctg catgcccgaa atgcaagagc aatcacataa
     3781 gaaatttcgg tacgggaacg caaa
//
LOCUS       DAOOJC010000237         3797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_18676_length_3797_cov_3.952699, whole genome shotgun sequence.
ACCESSION   DAOOJC010000237 DAOOJC010000000
VERSION     DAOOJC010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18676_length_3797_cov_3.952699"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="PLA01_07035"
     CDS             <1..176
                     /locus_tag="PLA01_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HPD01091.1"
                     /translation="GSIDSSDLQLLKRHLLRKSLLTGASLANADVNRDGSVDSTDCTL
                     LKRYILRIIRSFD"
     gene            545..1888
                     /gene="trkA"
                     /locus_tag="PLA01_07040"
     CDS             545..1888
                     /gene="trkA"
                     /locus_tag="PLA01_07040"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HPD01092.1"
                     /translation="MKVLIVGAGKLGYKLASQLSGGDTLVTVMDSDPEVLERISDHLD
                     VMIVRGSGMQVDALKEMNIEEYDLVIAVTENDETNMVICSIAKKLGCKKSIARVRNPE
                     YSEQGEFIRTIMGIDHIVNPELATANEINRNLLYHYSLYSGDFAEGRILMLHFTASSL
                     PEFVGKKIKEIKNISGILIGIISRNGDIIIPHGEDYILEDDILYVIGKKDNINQLAKT
                     CKTSVQIKYIKKVMILGGGRIGYYLAEKLTSQGISVKLIEYDKERCEYLSENLNDTLV
                     ICGDGTDVNLLNEEDISSMDAFVGVTGIDEENLLMTLMAKHVGVKKVIAKVSKSSYIQ
                     VIEKIGVDIAVSPIDITASDILKYIRGGRAASVSLLLGGKAEVTEVIADKDMWFVEKP
                     ISELGLPKEIIIGAVDHKGKVIIPTGSTIIQPGDRFVILSSAIRQKALEEFFKSL"
     gene            1903..3351
                     /locus_tag="PLA01_07045"
     CDS             1903..3351
                     /locus_tag="PLA01_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPD01093.1"
                     /translation="MNYGIVIKTLGKILVLEAVMMIFPSVVSLLYYQQDKVAFAVTLA
                     VTGLVGIFLSRFPAKTNTIKIREGLTIVTFAWILVSFFGSLPFVFSGSVDSFADAFFE
                     TVSGLTTTGATVIDNVEALPRGILFWRSFTHWFGGMGILVLMVSILPALGVGGFQVFK
                     AESTGPVTDRIVPRIKDTAKILYTIYLGITVLGVVLLIIGGMSLFDALVYSFGTVGTG
                     GFATKNLSVGAYNSVYIDIVISLLMLASGVNFSLYYSMRKKKWREVLVDSELRFYLGV
                     VGASVLLIAFNIFKNVYGSIWEALRYALFQVSSIITTTGYSTANFDEWPTFSKVILFV
                     LMFVGGCAGSTAGGMKSVRVLVLLKMIKREIMKIFHPRAVIPVKLGQRALSKDTLDGI
                     SSFFALYMFLFVLGTLLVSLDGFDLITSSSAVAATLGNIGPGFGAVGPIHTFSQFSHF
                     SKILMSVLMLLGRLGLFTVIALFSSKFWREDI"
BASE COUNT         1132 a          566 c          878 g         1221 t
ORIGIN      
        1 acggaagcat cgactcgtca gatcttcagt tattaaaaag acatttgctc cgtaaatctc
       61 tgcttacagg agcgagtctt gccaatgccg atgtaaacag agacggctct gtggattcta
      121 ctgactgtac attgttgaaa aggtatatac tgcgaattat tcgttccttt gattaacaaa
      181 gatcggtacc cgatataaat atgggagcat tattaaataa tcataccgga cctccacatt
      241 aatataaatc aatctaacaa aagctatggg ggcatcgagc ccccgtcgct ttttgggcat
      301 ttaacttcgt gaaatcccac taataagact ccggaagttt gtataacttt ctttcggagt
      361 tttatacttt gagaaagtac attgttttat acaaaaaatt tgcttttcgg catacttttt
      421 agttggaaaa tattttaatt tttcaataaa ttcatcttta attttatatt aaatgtgcta
      481 taatcgatgt taatgttaat aattatcaaa attaagccaa tgtagaggag agtaatgtat
      541 taatatgaaa gttttaattg ttggggcggg caaactggga tataagcttg cgagtcaatt
      601 gtccggagga gacactcttg taaccgttat ggattctgat ccggaggtat tggaacgtat
      661 ttccgaccat cttgacgtta tgattgtcag aggcagtggg atgcaggtcg atgcactcaa
      721 agagatgaac attgaggaat atgatctggt tattgcggtt actgaaaatg acgaaacaaa
      781 tatggttata tgttccatag ccaagaaact agggtgtaaa aaaagcattg cgagagtcag
      841 aaatcctgag tattcggagc agggtgaatt tatcaggacg ataatgggta tagaccatat
      901 tgttaatcct gaattggcta ctgcaaatga aataaacagg aatctgttat accattattc
      961 tttgtattcg ggggactttg cagaaggaag gattttgatg cttcatttta cggcttcaag
     1021 cctgccggaa tttgtgggta agaaaataaa agaaataaaa aacataagtg gaatattgat
     1081 tggaattata tcaagaaatg gtgatattat tattccccat ggagaagatt atattttgga
     1141 ggatgatatc ttatatgtaa taggcaagaa agataatata aaccaactgg caaaaacctg
     1201 caagacatca gttcagatta aatacattaa gaaggttatg atactgggtg gaggaagaat
     1261 agggtattat cttgcagaga aactaacgtc ccagggtatt tccgtcaagc tcatagaata
     1321 tgacaaggaa cgttgtgaat atctgtccga gaacctgaat gacaccttgg taatatgtgg
     1381 agatgggacg gatgttaacc tcctgaatga agaggatatt tcatctatgg atgcttttgt
     1441 cggagttaca ggaatcgatg aggaaaacct gttgatgact ctaatggcaa agcatgtggg
     1501 tgtaaaaaaa gtaattgcaa aggtaagcaa gtccagttat atacaggtta ttgaaaagat
     1561 tggggttgac attgcggtaa gtcctataga tattacagcc agtgatattt tgaaatatat
     1621 ccggggtgga agggctgctt ccgtatctct tcttttaggc ggaaaggcag aggtgaccga
     1681 agtgattgca gacaaagaca tgtggtttgt cgaaaagccg atatcggagc tgggcttgcc
     1741 taaggaaata attatcggag cagttgatca caaagggaag gttattatac caaccggaag
     1801 cacaattatc caacccggtg acaggttcgt aatactaagc tcggcaattc ggcaaaaggc
     1861 tctcgaagag ttctttaagt ctttataagg gagggctgtt cagtgaatta tggtatcgta
     1921 ataaaaacac tggggaaaat tctggttctt gaagcagtaa tgatgatttt cccatcagtt
     1981 gtatcccttt tgtattacca gcaagataaa gtggcatttg ccgttacctt agcagtgaca
     2041 ggattggtcg gtatctttct gtcacgtttt ccggctaaga caaatactat aaagataaga
     2101 gaaggtttga ctattgttac ctttgcttgg attctggtat cttttttcgg ttcattgccc
     2161 tttgtatttt cgggaagcgt agattctttt gcagatgctt tttttgagac agtgtccggt
     2221 ttaactacaa ccggggcaac tgtcattgac aatgttgagg ctttacccag ggggattctt
     2281 ttttggaggt cttttaccca ttggtttgga ggaatgggta tacttgtact tatggtttcc
     2341 attttaccgg ctttgggggt aggaggtttt caggttttta aggctgaaag cacaggtcct
     2401 gttaccgata gaattgttcc aaggataaag gatacggcaa aaatactgta tactatatac
     2461 ttaggcataa cagttttagg agttgtgttg ctgataattg gcgggatgtc attgtttgat
     2521 gcattggttt attcttttgg tactgtaggc acaggaggat ttgcaacaaa gaatttaagt
     2581 gtgggagcat ataatagtgt gtatatagat attgtaattt ccctacttat gctggcttcc
     2641 ggtgtaaact tttcattgta ttattctatg cggaagaaaa agtggagaga agtattagtc
     2701 gattctgagc tgaggtttta tttaggagtt gttggtgcaa gcgttctgct tattgctttt
     2761 aatatcttta aaaatgtcta tggcagcata tgggaagcgt tgagatatgc actttttcag
     2821 gtgagttcca ttataactac tacaggctat tcaacagcta attttgatga gtggccgacc
     2881 ttcagcaagg ttatactttt tgtccttatg tttgtaggag gatgtgcggg gtctacagca
     2941 ggcggaatga agagtgtcag ggttttggtt ctgcttaaaa tgataaaacg ggaaatcatg
     3001 aagatatttc atccgagggc tgttattcct gttaaacttg gtcagagagc attgtccaaa
     3061 gatacacttg acggtatatc cagttttttt gccctataca tgtttctttt tgtgctggga
     3121 acacttttag tatcccttga cggctttgac cttataactt cttcaagtgc ggttgcagcc
     3181 actcttggaa acataggacc gggttttgga gctgtgggtc cgatacatac tttcagtcag
     3241 ttcagtcact tcagtaaaat tttaatgagt gttttaatgc tgttgggaag acttgggctc
     3301 tttacggtaa ttgcattgtt ctcctcgaaa ttctggagag aggacatatg atactaagga
     3361 tgtatgatac ttttcatgtt attgtacaat aagccttgat aggcttattt ttttatgtat
     3421 agttgtctgt ttttattttt tggttagaca gatgagcaat atgtgctgtg ttgaagaaaa
     3481 acatattatt attgagataa gaaagcgtat tttatctcaa tgaatatatt taacgcatta
     3541 gttgggttga acatatgaca tgatagatga tatattagta aagctgtcgc tgatgacaca
     3601 ttgaagccta cgggttattg gttataagtg ttaataaaaa agtgttgaca aaacacaggc
     3661 ggcatgttaa cataagattc cgtgcttaag gaacagcaca atgcttgtta acttccgaca
     3721 gaagtattat gttaacaata gcatggactt tgaaaagtga acagtgtaag ataacatgaa
     3781 ctcgttaatg aaaatga
//
LOCUS       DAOOJC010000238         3796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_18692_length_3796_cov_3.385458, whole genome shotgun sequence.
ACCESSION   DAOOJC010000238 DAOOJC010000000
VERSION     DAOOJC010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18692_length_3796_cov_3.385458"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLA01_07050"
     CDS             complement(<1..289)
                     /locus_tag="PLA01_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01094.1"
                     /translation="MKKKRSCNNCLKGTAISLNCDILCIEKGVVSADYVCSKHRFMPA
                     LKSIKRKINTCVDCENFIIFDTTNIEDRAVGICQLFTVRKYDGKTKRVCSKF"
     gene            653..1129
                     /locus_tag="PLA01_07055"
     CDS             653..1129
                     /locus_tag="PLA01_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HPD01095.1"
                     /translation="MDVKKPIVLRKRYVPSETIDITGDELVLRNEELLVTKWKPIKKR
                     PDISGGMSFTFLKEGYKVSKFFDAFGEFKYWYCDIINVLYDEFNDKYTLVDLLLDVKI
                     TPDGHVEMLDMDEFAEALEKDIISKEEAVISLRTLDKILKMVYAGKFPPELCLKDY"
     gene            1241..2080
                     /locus_tag="PLA01_07060"
     CDS             1241..2080
                     /locus_tag="PLA01_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPD01096.1"
                     /translation="MDRFVDLHTHSTASDGSMTPSEIVRHAKKSGLSAVALTDHDTVD
                     GIEQAVEEGNKIGLEVVPGVEISLDYKTEMHLLAYFFDDSYMKIESLLERFRRSREER
                     NPKMIEKLNSLGIDITMEDVVAEAKGRVVARPHMANVMVRKGYVKTVAEAFEKYISNG
                     KPAYVKRDSLTPEKGIQAIREAGGIPVLAHPIYLRLDWGKLDELLSRLVSDGLMGIEA
                     YYVDNSQEDTGNLLNLAKKHNIVATGGSDFHGSFKPDIKIGSGKGNLKVPYEALERLK
                     ALA"
     gene            2329..2796
                     /gene="smpB"
                     /locus_tag="PLA01_07065"
     CDS             2329..2796
                     /gene="smpB"
                     /locus_tag="PLA01_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPD01097.1"
                     /translation="MVKEEKKVVAQNKKARHDYFIEQTIEAGIVLSGTEVKSIRTGKV
                     SLKDSYANIKDGEVFIYGMHISPYEQGNIFNKDPLRDRKLLLNRFEINKLIGYIQQKG
                     MTLVPLEVYFKKGRVKIELGIGKGKKLYDKREDIAKKDAQREIDRRLKDNSRY"
     gene            2873..3258
                     /gene="ssrA"
                     /locus_tag="PLA01_07070"
     tmRNA           2873..3258
                     /gene="ssrA"
                     /locus_tag="PLA01_07070"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            3510..>3796
                     /locus_tag="PLA01_07075"
     CDS             3510..>3796
                     /locus_tag="PLA01_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HPD01098.1"
                     /translation="MRDFDYTNCWQKLLTPDIVNLLTQIHEFKGEQTLFVEAKADTLT
                     QLMEIAKIHSTEASNKIEGIFTSDERLKKIVRDKTMPKNRNEQEIAGYRDV"
BASE COUNT         1235 a          633 c          856 g         1072 t
ORIGIN      
        1 caaatttgga gcagaccctt ttcgtttttc cgtcatactt acgtactgta aacagttggc
       61 atatacccac agccctgtct tcaatatttg tggtgtcgaa gataataaag ttttcacaat
      121 ccacgcaggt attgattttc ctttttatag atttaagggc gggcatgaac ctgtgcttgg
      181 agcagacata atctgccgaa accactccct tttcaatgca taatatatca caattcaggc
      241 ttattgcagt tcctttaaga caattattgc aagatctctt ttttttcaaa ttcttctccc
      301 cccaacccat tttctaatac tgaaattgtc tttttatgga caacttgccg ctaactgctt
      361 taatagttga gactcaggga atattaatag acatattacc ataaagctcc gtatatatga
      421 ttaatttatc agcttgaaat tgatgctgta taacactaca atattatagc attacaaggc
      481 aaactgtgga aggatgtggg aaataatatt gattaattaa ttttaaaatt gttatttgta
      541 aggtataata gataaataag ataataaata tttttgcacc ggtttcgggg caatattaag
      601 aagatggagt cttggaaaat ccgaataaac cggtgaaatt attacaaggg ggatggatgt
      661 gaagaagcca attgtactaa ggaaaagata tgtaccttcg gaaaccatcg atataacggg
      721 tgacgaactg gttttgagaa acgaggagct tttggtaact aaatggaagc caattaagaa
      781 aaggccggat atttccggag ggatgtcctt tacttttctg aaagaaggat acaaggtcag
      841 caagtttttt gatgcctttg gagagtttaa gtactggtac tgcgatataa taaacgtttt
      901 gtatgacgag tttaacgaca aatacaccct ggtggatttg cttttggatg taaaaataac
      961 tccggacgga cacgtggaga tgcttgatat ggacgaattt gcagaggctc tggaaaagga
     1021 tattatatca aaggaagagg ctgtaatttc acttcgaacc ctggataaaa tacttaaaat
     1081 ggtctatgcg ggcaaatttc ccccggagct ttgccttaag gattattaga tttttgtgca
     1141 attcatattg ttaaggaaat tcttttgaac acattaaaat ttatttagac tttcgcgata
     1201 ttcatattga tactattatt attgtaagga gcattgagcg atggatagat ttgttgattt
     1261 gcacacccat tccactgcat cagacggcag catgacaccc tccgaaattg taagacatgc
     1321 aaagaaaagt ggcttatcgg cggttgcact gacggaccat gatactgttg acggaataga
     1381 gcaggctgtt gaagaaggaa acaagatagg cttagaggtg gttcccggtg tggaaataag
     1441 tcttgactac aaaacagaaa tgcatttgct ggcttatttt ttcgatgaca gctatatgaa
     1501 aattgagtcc cttttagaga ggttcagaag gagccgggag gaaagaaatc ccaaaatgat
     1561 tgagaagctc aattctctgg ggattgatat taccatggaa gatgttgtcg ccgaagccaa
     1621 gggaagagtt gtggcaagac cgcatatggc taatgtgatg gttagaaagg gttatgtaaa
     1681 aaccgttgcg gaagcctttg aaaaatatat atccaacgga aaaccggcat atgtaaaaag
     1741 agattcattg actccggaaa aagggataca ggccatacgg gaggcagggg gaataccggt
     1801 actagcccat cccatttatt tgcggcttga ttgggggaag cttgatgagc tgctgtcaag
     1861 actggtttcc gatggactta tgggcattga agcatattat gtggataact ctcaggagga
     1921 cacgggaaat cttttgaatc ttgcgaaaaa gcacaacatt gttgccacag gagggagcga
     1981 ttttcacgga agctttaaac ccgatattaa aattggcagc ggcaagggaa atttaaaagt
     2041 tccctatgag gctcttgaaa ggttgaaagc ccttgcctga taatcctgat aatattgtaa
     2101 agcctacgaa ggtaagtcat aaatcccata cccttgtata agaatttaca gggcgggatt
     2161 atatggagca aacaggcagc aaacaagaaa atcagggtac ttgacagaag acatagaaat
     2221 gttataataa tagggcacga taaatatgtt gtgaaacatg ccctttctct gggaagggtt
     2281 atttttatcc ggtcaatctg taacattaaa ctgacaggcg gtaatataat ggtaaaggaa
     2341 gaaaagaaag ttgtagctca aaataaaaaa gccaggcatg actattttat cgaacagacc
     2401 attgaagcgg gcattgtttt gtcgggtacc gaagtcaagt cgatcaggac cggcaaggtt
     2461 agcctcaagg acagctatgc caacataaaa gacggtgaag tgttcatata tggcatgcat
     2521 ataagtcctt atgaacaggg gaatatattc aataaagacc ctttaagaga caggaagctt
     2581 cttttaaaca ggtttgaaat taacaaactc attggatata ttcagcagaa gggaatgaca
     2641 ctggtgccgc tggaggttta ttttaaaaag ggcagggtaa agattgaact gggtattgga
     2701 aaaggtaaaa agctttatga caagagggaa gatattgcaa aaaaggatgc acaaagagag
     2761 attgacagaa ggctgaagga taactccaga tattgatgaa gaaaatgcca agtactcgtg
     2821 aattaataca cgggcggggc aaagtctttg tgaaaactta ataattatac atgggggcgt
     2881 attggtttcg acgggattgt tgaagcttga gtagcgggta gaggattctc gttggcctct
     2941 ttaaaaaacg agaaactaaa ataaacgcta aaaacgataa tttcgcttta gctgctgcct
     3001 aatttaggcg gcccgtcagt ccggggttcc tgcatcccgg gtagctggcg tcattaagtc
     3061 aggccttttg agcgggaagg ttaaactcta gaactatcgc agctgtagcc ttgaactgcg
     3121 acggatttta aaggataccg atatctaaag cctgcccttg ggcgttaggt tgagggaatt
     3181 ttaaatcgag ggctgcaccc ggagaagctc ttgtgaatat ggtttcggac aggagttcga
     3241 ctctcctcgc ctccaccaaa atttttatga aaacccttgc attgcaaggg ttttcggaca
     3301 atctggccag tattgcctac aattgaacct attcttaata tcgtttttta tttctgtata
     3361 gcacattttc tatattatca tctctgattt aatttaatca tttctatcaa tatgattaaa
     3421 ttcatattga caatttaatt ttataatgat acactttaat ttaactattg ggcaataata
     3481 tgattaaatt gaccattgcg agggataaaa tgagagattt tgattacaca aactgttggc
     3541 aaaagctttt gacaccggat attgttaacc tgctgacgca aatccatgaa ttcaaaggtg
     3601 agcaaacact gtttgtagag gctaaagccg atacattgac acaactcatg gaaatagcaa
     3661 aaattcatag cactgaagct tcaaataaaa tagaaggcat tttcacttcg gacgaacgac
     3721 tgaaaaaaat cgtccgtgat aaaaccatgc cgaaaaatcg aaatgagcag gaaattgccg
     3781 gttacagaga cgtttt
//
LOCUS       DAOOJC010000239         3778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_18818_length_3778_cov_3.781091, whole genome shotgun sequence.
ACCESSION   DAOOJC010000239 DAOOJC010000000
VERSION     DAOOJC010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18818_length_3778_cov_3.781091"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..230)
                     /locus_tag="PLA01_07080"
     CDS             complement(18..230)
                     /locus_tag="PLA01_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01099.1"
                     /translation="MKKSIKLICVFLALGVLIAYCAVITSVSANKNQYKTDMLIFLEQ
                     EKERLEKNNEEVPEEVLRYFVWVNDM"
     gene            complement(752..3094)
                     /locus_tag="PLA01_07085"
     CDS             complement(752..3094)
                     /locus_tag="PLA01_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPD01100.1"
                     /translation="MVKCSMCKENLAVVFITKIVNGKQTQEGLCFSCAKKRGIQPINQ
                     ILEQTGISEDEIDDLNKQMGSFLEEMDMNSGDEDGADIELPNNANSLFNLINKSLLKS
                     GDNAGLKDSKDLKDEDKEEKEEKEKDNKNNNTRTKTQEKKIPKKKKYLDAYGINLTAK
                     AKEGKIDRVIGRQREIDRVIQILNRRSKNNPVLIGEPGVGKTAIAEGFAVRIAERDVP
                     AKLFNMEVYVLDLTSIVAGTQFRGQFESRMKGIIEECKALGNIILVIDELHNIMGAGE
                     AEGAMNAANILKPVLAKGEVQVIGATTLNEYRKHIEKDSALERRFQPVLVDEPTVDET
                     IEILRGIKDYYESYHRVRISDEVLRAAVVLSERYITDRFLPDKAIDVLDEAGSRANIK
                     NVGLVEFEALKEELKKVQEEKENAISADSIEDYQKAADLKVRECKLLQQIKEIEYKNK
                     DMEMTLEDVAYVIESWTKIPVQRLTEVEAKKLLNLEERLHNRVIGQENAVSSVAKAIR
                     RNRADFKKKKKPASFIFVGPTGVGKTELVRALAVELFESEEALIRLDMSEYMEKHTVS
                     KLIGAPPGYVGYDEGGQLTEKVRRKPYSVILLDEIEKAHPDVFNMLLQVLEDGRATDS
                     HGRTVSFENTVLIMTSNAGTSLKANGIGFANDSYIALENKIKDVLKETFRPEFLNRID
                     EIIIFNELNQEELRQIIDLMLKDVIDEVENKNMNISFSDEVKKFILEKGYDPKYGARP
                     LRRTIQKYIEDELTDLYLKGVYKEGSTIEVDVENGSIVFR"
     gene            complement(3390..>3778)
                     /locus_tag="PLA01_07090"
     CDS             complement(3390..>3778)
                     /locus_tag="PLA01_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPD01101.1"
                     /translation="YDVGLGYIKLGQSSTTLSGGEAQRVKLSTELSKRSTGRTMYILD
                     EPTTGLHIADVHRLIGILHRLVDAGNSVLVIEHNLDVIKTADYIIDLGPEGGNKGGMI
                     VAQGIPEEIIKVEESYTGQFLKKMLK"
BASE COUNT         1047 a          919 c          493 g         1319 t
ORIGIN      
        1 ccataaaagt tgtattttta catatcattt acccacacaa aatagcgaag cacctcctca
       61 ggcacctctt cattattttt ttcaagtctt tctttttcct gctctaaaaa aattagcata
      121 tctgttttat attgattttt atttgcagat actgaagtta ttacagcaca atatgcaatc
      181 aaaacaccca atgctaaaaa tacacaaata agtttaattg actttttcat tttcactcat
      241 ctccttatat attaataata cttaattcac ttgtgctgga gggggtaaag ctatccatga
      301 acgatattca atggattctc ccaaacctac attagatagc caataattaa acctattata
      361 tgattgttgg tttttaaaag tataatttga ccaggcatta caactcatta caccttcaaa
      421 atataaacct ctccattttt tttgatatac ccactgctta gcacaatcct ctacatagat
      481 ctgaggaaaa caataactgc atctattacc ccaagatata taaagaccaa aaagtagagg
      541 aactcctatc acaaccttaa cagatactgc cttttactaa aattaataaa aacgtaataa
      601 ttagtcagac tattttatga tataatgata ttgaactact gaaaagtgat tcaaaagaaa
      661 aacctataac attttttcat acacaatctt tatagcataa gctccggcga catcgaacct
      721 ccggagcttt tcacttattt cactcaagtc tctatctaaa aacaatactt ccgttttcaa
      781 catcaacctc tatagtgctt ccttccttat acacaccctt aagatacagg tcggtaagct
      841 catcctctat atatttttgt atagtcctcc tcaacggtct tgcaccatat ttgggatcat
      901 agcccttttc aaggataaat ttcttaactt catcactgaa actaatgttc atatttttat
      961 tttctacctc atcaataaca tccttcagca tcaagtcaat tatttgcctg agctcttcct
     1021 gattgagctc attaaatatt attatctcat ctattctgtt tagaaactca ggtctgaagg
     1081 tctccttcaa aacatccttt attttattct caagagctat ataactgtca ttggcaaaac
     1141 ctatgccgtt tgccttaaga ctggtcccgg cattagacgt cattatcagt actgtgtttt
     1201 caaagcttac tgtcctacca tggctgtcgg ttgcccttcc atcctccaaa acttgcagga
     1261 gcatattaaa tacatccgga tgggctttct caatctcatc caacaggatt accgaataag
     1321 gcttccttct caccttttct gtcaattgtc cgccctcatc gtatcctaca tatcccggag
     1381 gtgctcctat caactttgac accgtatgct tctccatata ctcagacata tcgagcctta
     1441 tgagggcttc ctcactttca aagagctcaa cagccagtgc cctgaccagt tctgttttac
     1501 ctacaccggt gggtcccaca aatataaagg aagccggttt tttcttcttt ttgaaatccg
     1561 ccctgttcct tcttatggct ttagccacac tggatacggc gttttcctgg ccaataaccc
     1621 tgttatgaag cctttcttcc agattaagca gtttttttgc ctctacctca gtaagacgct
     1681 ggacaggaat tttagtccag gactctataa catatgccac atcttccaac gtcatttcca
     1741 tatccttatt cttatactct atttccttta tctgttggag aagcttgcac tctcttacct
     1801 taagatcggc agccttctga taatcctcga tggaatctgc cgaaatagca ttttccttct
     1861 cctcctgtac ttttttcagt tcttctttta atgcttcaaa ctctacaagt ccaacatttt
     1921 ttatattagc cctcgagcct gcctcgtcca atacatctat tgctttgtcc ggtaaaaacc
     1981 ggtcggtaat atacctttcg gaaagcacaa cagcagcccg taatacttcg tcagaaattc
     2041 taaccctgtg gtaactttca taataatcct taatacctct tagtatctct atcgtttcgt
     2101 caactgtagg ctcatctacc aaaacaggtt gaaatcttct ttccagtgca gaatccttct
     2161 caatatgttt cctgtactca ttaagagtcg tagcacctat cacctgaacc tcacccttgg
     2221 caagaacagg ctttaaaata ttggcagcat tcatagcccc ttcggcttct cccgctccca
     2281 ttatgttgtg aagctcatct ataacaagaa taatatttcc caacgctttg cattcctcaa
     2341 taatcccttt catacggctt tcaaactgcc ctctgaactg tgtccccgcc actatgcttg
     2401 taagatccaa aacatatact tccatattga acagctttgc cggaacatcc ctttctgcaa
     2461 ttcttactgc aaatccctcc gcaatagctg tttttcctac tccgggttca cctatcaaca
     2521 caggattatt tttacttctc ctgttcagga tctgaatcac cctgtcaatt tccctttgtc
     2581 ttccgatgac acggtcaatt ttcccttctt ttgcttttgc tgtcaaattt ataccgtatg
     2641 catcaagata ctttttcttt ttcggtatct ttttttcctg ggtttttgtc cttgtattat
     2701 tgttcttatt atccttttcc ttctcttcct tttcttcctt atcttcatcc tttaagtctt
     2761 ttgaatcttt taatcctgca ttgtcaccag actttaataa ggatttgttg ataaggttaa
     2821 acaacgaatt ggcattgttc ggcaattcta tgtcagcccc atcttcatcc ccactgttca
     2881 tatccatttc ctctaaaaag ctgcccatct gtttatttaa atcatctatt tcatcctcgg
     2941 aaataccggt ttgctctaat atctgattta ttggctgaat tcctcttttc ttagcacaag
     3001 aaaagcacaa tccttcttga gtttgcttac cattcacgat tttcgttata aaaacaacgg
     3061 ccagattctc tttgcacatt gaacacttca ccatatctat catctccttt tcttagccag
     3121 tctaaaagta cagtaaaata actccatatc taattaataa aattcctaca tatatatctt
     3181 attcggattt atttcaaaat catatatata aaactattga acgcatatta cggaatgtaa
     3241 tttacattct gcgttcaata taaagtataa catatattta tatttccggt ctaataatat
     3301 tcagttcctt ttaagcccgt aattacaaat aattcttata taaatcacaa taaatggtta
     3361 catcttcgtt ttttatttat gctttcaacc tacttcaaca tctttttcag aaactgcccg
     3421 gtatacgact cctcaacctt tattatttcc tccgggatac cttgggcaac tatcatacca
     3481 cccttgttgc cgccctcagg acctaaatcg attatataat cggcagtctt tatgacatca
     3541 agattgtgtt ctattaccaa cacagaattt cctgcatcca ccagcctgtg aagaatgcct
     3601 ataagtctgt gtacatcagc aatatgaagt cctgttgtcg gttcatcaag tatatacatt
     3661 gtccttccgg tacttctttt tgaaagctcg gtagaaagtt taaccctctg ggcctcgcct
     3721 ccggaaagag ttgttgaaga ctggcccagt tttatataac caagccccac atcataca
//
LOCUS       DAOOJC010000240         3753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_18970_length_3753_cov_2.590860, whole genome shotgun sequence.
ACCESSION   DAOOJC010000240 DAOOJC010000000
VERSION     DAOOJC010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18970_length_3753_cov_2.590860"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLA01_07095"
     CDS             <1..228
                     /locus_tag="PLA01_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01102.1"
                     /translation="QQGLKLHLQPGKFWLSVSVIGKRVNSTKYEFVLGSFYKQSPTGS
                     ESIVWNMNLFISDKSVEFTTSLSRYTYAALS"
     gene            482..1375
                     /gene="queG"
                     /locus_tag="PLA01_07100"
     CDS             482..1375
                     /gene="queG"
                     /locus_tag="PLA01_07100"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HPD01103.1"
                     /translation="MKKALIEFCKSIRLEHVGIAPSGPYNDLKDLWEKRIEKGFVHAF
                     NKGSLEKMICPNLTLEDAKSVIVCLFPYYCGNFENANLSKYAYSVDYHITVKNMLEKI
                     GDFLQKKIQGFNYEAFADTGPLNDRYLAYKAGLGFFGINNHIITDEYGSYVFIGYIIS
                     NYPFEPDKPQDRTCRKCFDCVKRCPEQCILGDFTINPFKCRSYISQKKGELSAEDMEI
                     LKKNPLIWGCDVCQDVCPHNQKVKKTVMEEFKHNIKYRIEYEELEEISNNEFFRRYKD
                     RAFSWRGKGVLKRNYEIINEL"
     gene            complement(1517..3139)
                     /gene="metG"
                     /locus_tag="PLA01_07105"
     CDS             complement(1517..3139)
                     /gene="metG"
                     /locus_tag="PLA01_07105"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPD01104.1"
                     /translation="MNIFIGGAWPYANGSLHIGHVAALLPGDILARYFRLKKYNVCYV
                     SGSDCHGTPIQIRAQKEGLSPEEIADRYHNEFKECFKKLGFSYDLYSQTKDPYHIAFV
                     QEFFTNLLNSGFVYRKAVEQVYCESCRQFLPDRFVVGTCPNCKSKTKGDQCDACGTLF
                     DPANLKDRKCGICGDTPVFKLSEHFFLALSKLQSFIEDYVQNSSGWRTNALGLSQRYI
                     EGLRDRAITRDLEWGISVPLKGFETKKIYVWVEAVLGYLSASKKWAEACGVNWHEIWS
                     ENSLHYYIHGKDNIPFHTVILPALIKAHGGLHLPDRIISSEYVTLEDRKISTSENWAV
                     WIPYLIEYYNPDSIRYYFIANGPEKKDTDFSWREFINSHNGELLGAYGNLVNRSLVFV
                     QKSFDGKVPKGECSPNIKKTLADLYPEVGSLIEQGDFKDAIEKVFSFIRSANKYFDEE
                     QPWITLKENIEKCRQTLFTCVQIIANLSILLEPFLPFSSSKIRSMLHIESTGWKYTEV
                     PFDQIIEDVNILFERIDKKRIQEEIENMKRVN"
     misc_binding    complement(3287..3547)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1172 a          685 c          722 g         1174 t
ORIGIN      
        1 cagcaaggct taaagctgca tctccagccc ggtaagtttt ggctttctgt gtctgtcata
       61 ggtaagagag tcaatagtac caaatatgaa tttgttttgg gatcgtttta caagcagtcc
      121 ccaacgggta gtgagtcaat agtatggaac atgaatcttt ttatcagtga caaatcagtt
      181 gaatttacta cttcactgtc aagatataca tatgctgctc taagctgatt acttgttaaa
      241 ctgatgaccc taaggaggtt cgaacctttc atatttaaat acgaacgcaa gattttattt
      301 cagcgggaat ttattaaaat aaataagact atttcctatc atgaagaagt ttagtggaaa
      361 agtcaggctt ataatttttt gaaatccaga taaaggtgaa ggtataatgt aaataaataa
      421 tatataatgt aagcaaaatg gatgttacat aataaaaaaa cattgaattg ggattgatgg
      481 gatgaagaaa gctttaattg aattttgtaa atctataagg ttagaacacg tagggatagc
      541 accttccggt ccttataacg atttgaagga tttgtgggaa aagaggattg aaaagggctt
      601 tgtgcatgct tttaataaag gaagtttgga gaaaatgatt tgcccgaatt tgactttgga
      661 ggatgcaaaa tcggtaatag tgtgtttatt tccctattat tgcggtaatt ttgaaaatgc
      721 caatttatca aaatatgctt acagtgtgga ttaccatata acagttaaaa atatgctgga
      781 gaaaataggg gattttttgc aaaaaaagat tcaagggttt aattacgaag cctttgcaga
      841 cacaggaccg cttaatgaca gatatctggc ctataaagcg gggttgggct ttttcggaat
      901 taacaaccat ataataacgg atgaatacgg ctcttatgtt tttataggat atattataag
      961 caattatccc tttgagcctg acaaaccgca ggacaggaca tgccgaaaat gctttgattg
     1021 tgtgaaaaga tgccccgaac agtgcatttt aggtgacttt acaataaatc cgtttaaatg
     1081 caggtcttat atatctcaaa agaagggcga actttccgcg gaagatatgg agattttaaa
     1141 gaaaaatcct ttgatttggg gatgtgatgt atgccaggat gtctgtcccc acaaccaaaa
     1201 ggtaaaaaag acggtgatgg aagagtttaa gcataatatt aagtacagaa tagaatatga
     1261 agagcttgaa gaaatttcta acaatgagtt ttttagaaga tacaaggata gggcttttag
     1321 ttggagaggc aaaggagttt taaaaagaaa ttatgaaatt attaacgagc tatgaagtgt
     1381 tttaacaagt tgcaaatcat atatgtgaag aaaaacgtga aagaaactta aattttcttt
     1441 tacacttttc ttcacattta gcgaaatttc gcgaagtaaa attaccggaa gagtgaaata
     1501 acccgatgcc cttgagctaa ttcactcgct tcatattttc aatttcttcc tgaattctct
     1561 ttttatcgat gcgttcaaaa agaatattta catcttcaat aatttgatca aacggcactt
     1621 cagtgtattt ccaaccggtt gattctatat gaagcatgct gcggattttt gacgatgaaa
     1681 agggcaggaa gggctccagt aaaattgata gattggcaat aatttgaaca caggtaaaaa
     1741 gggtttgcct gcatttttct atgttttcct taagggtgat ccatggctgt tcttcgtcaa
     1801 aatacttgtt cgctgagcgt ataaatgaaa acactttctc aatggcgtct ttaaaatctc
     1861 cctgttcaat gagacttccg acttccgggt ataaatcagc taatgttttc tttatattag
     1921 gactgcattc tcctttgggc accttgccgt cgaaagactt ttgtacaaag acaaggctcc
     1981 ggttgactaa atttccgtaa gcaccgagga gttccccgtt gtggctgttt ataaattctc
     2041 tccatgaaaa gtccgtatct tttttttcgg gaccattggc aataaagtaa taacggatac
     2101 tgtccggatt ataatactca ataagatacg gtatccagac cgcccagttt tcactggtgg
     2161 atatctttct gtcctcaagg gttacatact cgctggatat tattctgtcc ggcagatgca
     2221 aaccaccatg ggcttttata agggcaggga gaattacggt gtgaaaggga atattgtctt
     2281 ttccgtgtat ataatagtgt aaagagtttt cggaccatat ttcatgccaa tttacaccgc
     2341 aagcttctgc ccattttttg ctggcggaaa gatatcctaa tactgcttca acccacacat
     2401 atatcttttt ggtttcaaac cctttcagag gcacggaaat accccattcc aaatcccggg
     2461 ttattgccct gtcacgaagt ccttcaatat atctttggga tagtcccaat gcattagtcc
     2521 tccagccact ggaattttgg acataatcct caatgaagga ctgcagcttt gataatgcca
     2581 gaaaaaagtg ttccgataat ttgaagacgg gagtatctcc acagataccg cattttctgt
     2641 cttttagatt ggcaggatca aaaagagttc cacaggcatc gcattgatcc ccctttgtct
     2701 tgcttttaca atttgggcaa gtaccgacaa caaacctgtc gggcagaaat tggcggcagg
     2761 actcacaata aacctgttcc acagctttcc tatatacaaa accgctgttg agaagatttg
     2821 tgaaaaattc ctgaacaaaa gcaatatgat agggatcctt tgtttgactg tacaaatcat
     2881 atgaaaagcc cagctttttg aaacactcct tgaactcatt gtggtaacga tccgcaattt
     2941 cttcagggga aagcccttct ttttgagccc gtatctgaat gggtgtgccg tgacaatcac
     3001 ttcctgaaac ataacacaca ttatattttt ttaatctgaa atatcttgcc agtatatctc
     3061 ccggcagtaa agccgcaaca tggcctatgt gaagagatcc atttgcataa ggccaggctc
     3121 cgccgatgaa tatgttcata aacaactcct ccttaaatta ctgtttttta aataaaactt
     3181 ttttgtacaa acaaaaagtc aagctctaag tgccctgatt ttttcgtttg ctttgctaaa
     3241 actaaataga tacgaataaa aaagccctca cccccaggca atttatccta gggacgagag
     3301 cattatgctt cgtgttacca ccccggttca ccgatatttc gcaatatcgg cctcagcaag
     3361 tacgtcagaa acttctgatt ataccctggc actataacag gtgcacctgt cgcagcctaa
     3421 atccatcgga tagtcggtgc gaaagctcca agaccatgtt cggctatctg tactttgctc
     3481 cttctcagct tcccggagtt ctctgtaaaa gccatcaata gcgtactctt ctcttcatcg
     3541 catacttagt attaccatat taattacatt ttaatacaga taattatgta agtcaatatg
     3601 tttagatttt aatagtttat tacatggaca tcaaacaagt gtatagttat gtatagataa
     3661 aatttattct atttccatat tatactttac ataatcaata tacacccgtt ttttttcgat
     3721 aatgttcggt ttttagcata tgagtatata aat
//
LOCUS       DAOOJC010000241         3738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_19074_length_3738_cov_3.096660, whole genome shotgun sequence.
ACCESSION   DAOOJC010000241 DAOOJC010000000
VERSION     DAOOJC010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19074_length_3738_cov_3.096660"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PLA01_07110"
     CDS             complement(<1..735)
                     /locus_tag="PLA01_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HPD01105.1"
                     /translation="MRKTPYSELESRMKRFRAVMDEHNPGWEMAVVFSKVNLYYFTGT
                     MPEGMLLIPRDDEAVLWVRKSVERAQDESHFPLIKPMNSFRDAARAFTDIPETIYLET
                     EFVPIAFYKRFQKYFPFENVKPLDRQISFLRSVKSRYEMDIMEQSGVIHRRILEDRVP
                     KILEEGISEAEFAAKLYSVMVEEGHHGIARFNMFDTEILVGHVCFGESSIYPTYFNGP
                     GGNYGMSPAVPLLGSHDRRLKKGDLVF"
     gene            complement(997..3702)
                     /locus_tag="PLA01_07115"
     CDS             complement(997..3702)
                     /locus_tag="PLA01_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD01106.1"
                     /translation="MNIFYKVTLQMLKKNKIRTLVTIIGIILSASMLTAVTTSVSSLQ
                     DFLLDVAIAQDGDWHGAVHYIKKAQIDKLDEDPDVKSYTWMQNIGYAMLEESRNDYMP
                     YLFIAGMDNSFKDVMPVNLIEGRMPENTAEIILPQHLETNGGVKHSIGDVLVLDIGSR
                     ISDGFELNQDSGYRNGENGGIKETEELVIREQRTFTVVGFYERPSFEPYVGPGYTALT
                     VSDNTGADNYDVYIKVKKVKNIYNYLDTRFPQNERRTNRDYLIFNGVSDDTGFNAVLY
                     GLAGVLIILIMFGSILLIYNAFSISVSERTKQFGLLSSIGATKRQLLRSVLFEAFFLS
                     TIGLPLGILSGIAGIGITLELTQNMFMSFLQYNTDVVMDLYVSGSAVAASALVGLITV
                     LISAFIPARRAVRVSAIDAIRQTNDIKIKAGKVKTSKLTYKLFGFEGMIARKNYKRNR
                     KKYRATVISLFMSVVLFISASSFCAYITKGVDSVIDDEEYDIIYSFTPDQREKCSLEE
                     LFMELSEVKGVKDSSYVYFQHKTIEIPVNSLNKEYISFSKRIFGENYPNYQSEHETFD
                     AVLYFIDDSTYEKFLNKNSYDKNLYFSPNSPLAVVRDFTKFYDGGKYYTFHILGSNPS
                     DIVFKQIKDKERFFFSGNVTDDDGKTVYLFRNKYPEDGEQELKLTFDEATEESNIKIG
                     VVSDKKPFCVDNNFGSTITFMYPYSAISAVTGKDTESVESFFYFKADDHKSVYNKICS
                     ILEDKGLPTSMLYDAAENGESMRALVSVINIFSYGFIILISLIAVANVFNTISTNIAL
                     RRREFAMLKSIGMTQKGFNKMMNYECLLYGIKGLIYGIPVSFGVTYLIYRSIAGGWET
                     KYFVPWYSVVIATGSVFAVVFSTMLYSMRKIKKDNPIDALRNENL"
BASE COUNT         1104 a          868 c          618 g         1148 t
ORIGIN      
        1 aaacacaagg tctccttttt taagccgtcg gtcatgactt cccaaaagcg gcaccgccgg
       61 gctcattccg taattgcccc cgggtccgtt gaaataagtg ggataaatcg aactctcccc
      121 aaagcacaca tgccctacaa gtatttcggt atcgaacatg ttaaaacgag ctattccgtg
      181 gtgtccttcc tcgaccatca ctgagtaaag cttcgctgca aactccgcct cacttatccc
      241 ttcctcaaga atcttcggga ctctgtcctc caatattctt ctatgaataa cccctgactg
      301 ctccattata tccatctcat aacggctctt tactgatctg agaaaagaaa tctgtctgtc
      361 taaaggcttc acattctcaa agggaaaata cttttgaaac ctcttataaa aagcaatggg
      421 aacaaactcc gtttcaaggt atattgtctc cgggatatcg gtaaaagctc gggccgcatc
      481 gcgaaagctg ttcatcggct taatcaaagg aaagtgtgat tcgtcctgtg ccctctcaac
      541 gctttttcga acccacaata ccgcctcatc atcccggggt attaatagca ttccctccgg
      601 catggtgcct gtaaaataat aaaggttaac cttgctgaaa acaaccgcca tttcccatcc
      661 gggattgtgc tcgtccataa ccgcacgaaa ccgtttcata cgggactcaa gttcactata
      721 aggtgtcttt ctcattacat tgtcctccta tgcaaaatga tccggataaa aattaaatta
      781 atccaaatcc ttacattttc cgaataactc aaattcataa gaattatatc atactcaata
      841 taggctgtgc catctttctt tgtttatacc gttacaaaag gccggtcacc tcattgaccg
      901 gccctttgca attattaaat tcatgatcta tttcatattt tttttaatag aaaaactctt
      961 tactttaatc atatggtctt gaataactgc atccttttat agattttcgt ttctgagtgc
     1021 atctatagga ttatcctttt tgatttttct catggaataa agcattgttg aaaacaccac
     1081 ggcaaaaaca ctgcccgttg caataacaac gctgtaccat ggaacaaagt atttcgtttc
     1141 ccaccctccg gcaatacttc gatatataag gtaggttaca ccgaaggata cgggaatacc
     1201 gtatatcaaa cccttaatac cgtaaagcag acattcatag ttcatcattt tattaaatcc
     1261 cttttgcgtc atccctatgg attttaacat cgcaaactcc cggcggcgaa gtgctatatt
     1321 tgtcgaaatg gtgttaaaca catttgccac tgcaatcaaa gaaattaaaa ttatgaaacc
     1381 ataggaaaaa atattgataa ctgaaaccaa tgcccgcatc gattctccgt tttcggcagc
     1441 atcatatagc attgaagtcg gcaagccctt atcttccaga atgctgcata ttttattata
     1501 tacggatttg tgatcatccg ccttgaaata aaagaaagat tccacacttt ccgtatcttt
     1561 accggtaaca gccgaaatag cactgtaagg atacataaat gttattgtgc ttccaaagtt
     1621 gttgtcaaca caaaagggct ttttatccga aacaacacct attttaatat ttgattcttc
     1681 cgttgcctca tcaaaggtta atttcaactc ttgttcaccg tcttcaggat acttatttct
     1741 aaacagataa actgtctttc cgtcatcatc ggtaacattt ccgctgaaaa aaaatctttc
     1801 cttatcctta atttgtttaa acacaatatc cgaaggattg gaccctagaa tatggaatgt
     1861 ataatattta cccccgtcat aaaatttcgt aaaatctctg acaaccgcca acggtgaatt
     1921 cgggctgaaa tacaggtttt tgtcataaga atttttattt aaaaacttct catatgtgct
     1981 atcgtcaata aaatacaata cggcatcaaa agtttcatgt tccgattgat aattgggata
     2041 gttttctccg aatattctct tgctgaaact gatatactct ttattgaggc tgttgaccgg
     2101 aatttcgatg gttttatgct gaaaatatac ataactgctg tcctttactc cttttacttc
     2161 ggacaattcc ataaacaact cttccaatga acacttttcc ctctgatccg gcgtaaagct
     2221 gtaaataata tcatactcct cgtcatctat aacagaatcc acaccctttg taatatatgc
     2281 acaaaaactg ctggccgata tgaataaaac cacactcata aaaagagaaa tcaccgtagc
     2341 ccggtacttt tttctgttgc gtttgtaatt tttcctggca atcattcctt cgaatccaaa
     2401 cagcttatag gtaagctttg aggttttcac ctttccggcc ttaattttaa tgtcattagt
     2461 ttgccttatg gcatcaatgg ccgataccct tacggcacgc cttgcaggaa taaacgccga
     2521 tatcagcacg gtaatgagac caaccaatgc cgacgctgca acagcagatc ccgaaacata
     2581 taaatccatc accacatcgg tattatattg taagaaagac ataaacatat tttgtgttaa
     2641 ctccagtgtt atacctatcc cggcaattcc cgacaatatt cccagaggaa gccctatagt
     2701 gctcaagaaa aatgcctcaa acagcacact tcttagtagc tgccgtttgg tggcaccgat
     2761 agaggataaa agtccaaact gctttgtgcg ttcactgacg gatatggaaa aagcgttata
     2821 tatcaataaa attgaaccaa acattatcag aataattaaa actccggcaa gcccgtataa
     2881 tactgcatta aatccggtat catccgaaac accgttaaaa ataagataat ctctgttggt
     2941 tcttctttcg ttttgaggga atcgggtatc cagataatta tagatgtttt taactttctt
     3001 tactttgata taaacatcat aattgtctgc accggtattg tccgaaacag tcaaagcagt
     3061 atatccggga ccgacgtatg gctcaaagct cgggcgttca tagaatccga ccacggtaaa
     3121 ggtacgctgc tcccttatta ccaattcttc cgtttctttt attccaccgt tttcaccatt
     3181 ccgatagccg ctatcctgat taagctcaaa tccatccgaa atccggcttc ctatatcaag
     3241 aaccagtaca tcgccgatgg aatgcttcac accgccgttg gtttcaaggt gctgaggtaa
     3301 aattatctcc gcggtgttct ccggcattct cccctcaatt aaattaacgg gcattacgtc
     3361 cttaaagcta ttgtccattc ctgcgataaa cagatacggc atataatcat ttcgggattc
     3421 ttccaacatc gcataaccga tattttgcat ccatgtatag gattttacat cgggatcttc
     3481 atctaattta tcaatttgtg ctttttttat ataatgcacc gcaccgtgcc agtccccatc
     3541 ctgggcaata gccacatcca gtaaaaaatc ctgcaggctt gatacagaag tagttactgc
     3601 ggtaagcatc gatgcggata aaattatccc gatgatggtt acaagggttc ttattttgtt
     3661 ttttttgagc atttgaaggg ttaccttata aaagatgttc atctgcgtat cacctcatca
     3721 cgagtgattt ttccgtct
//
LOCUS       DAOOJC010000242         3710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_19261_length_3710_cov_2.863201, whole genome shotgun sequence.
ACCESSION   DAOOJC010000242 DAOOJC010000000
VERSION     DAOOJC010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19261_length_3710_cov_2.863201"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..924
                     /locus_tag="PLA01_07120"
     CDS             139..924
                     /locus_tag="PLA01_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01107.1"
                     /translation="MSRITCFPEYKSRIVGYFRIRTIEYKDVDANLLFNEFEKCSFAI
                     PEFPPGNLDMWLHYQAHIFLMDKFKNSPDKFHLLYIELINKYGIFIKAAELEHFRAYY
                     PEELFFNLDFPTDMLRTLVCKARYPHRILCYCFRYLLHGWSAIRIASELNDYELDDML
                     DLFLKEYKKESGLPSFFVDYMFSPLRRILNEVLANYLSRQGKTDTKFIEVIQPITNHR
                     IGSTKLDNYFGVNELSQRSHMVSVWCNRIKNTLYSNYVAFLKN"
     gene            954..1463
                     /locus_tag="PLA01_07125"
     CDS             954..1463
                     /locus_tag="PLA01_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01108.1"
                     /translation="MNAEKTKCPSHETIMNYFNMELDETEAIKVEEHFSYCNECIGQF
                     NYYKEYIEQISLSASVQKELWHNSNVDFVSVSVADSSQVREVSELVTDKGKYTIRLIP
                     YQDEMSRALLVIEVADRNIKGDIKVFLLDKANLTHVGTEPLNEEHQACFEVPSGINLK
                     KLILKVENI"
     gene            1649..2959
                     /locus_tag="PLA01_07130"
     CDS             1649..2959
                     /locus_tag="PLA01_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HPD01109.1"
                     /translation="MELLKKLEILSAAAKYDVSCSSSGSNRKNTKGGIGNASACGICH
                     SWADDGRCISLLKILLTNYCIYDCAYCVNRVSNDVPRAAFTPREVADLTINFYKRNYI
                     EGLFLSSSIIKNPNHTMELLYESVRILREEYLFNGYIHIKAIPGADLNLIESTGRLAD
                     RISVNIELPSNKSLKLLAPQKEKEAIIKPMGFISSKIVEKKEERRLLKKAPAFAPAGQ
                     STQLIVGASPEHDLNILRLSEKLYQNFKLKRVYYSAYVPVSSQNPNLPAINTPPLLRE
                     HRLYQADWLLRFYGFSSDELLDEKTPDFDIHLDPKANWALNHMELFPVEINRADYEML
                     LRVPGIGVRSANKIIRARRVKSLNHDDLKKLGIVLKRARFFITCSGKYSGGIDMNDTV
                     IRYALMDKANRFDFTPTWEQLSLFSLIDNMPTVNDKIMSITGEM"
     gene            2959..3702
                     /locus_tag="PLA01_07135"
     CDS             2959..3702
                     /locus_tag="PLA01_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HPD01110.1"
                     /translation="MIYYYYDKSFEGLLTAIYEAYYRKESPYICPVQKNNQMSIFDTG
                     ITIETSEEKASKVYEAIYKKISYNALKNAYHAHLSEIDGISNSIYQYIKLGFKVGRKV
                     DLYLTDERVFPVHKAVKSVQRESHLMMGIIRFKKLDNDLYYASYEPDYNITVLLIPHF
                     SERFSNQNWIIHDIKRGIAAVYDKNDCVITDLPEEIYKCSKSRTDQYETLFKGFFKSI
                     CINERINPKLQKQYMPKRYWKHLIEKSDS"
BASE COUNT         1255 a          676 c          698 g         1081 t
ORIGIN      
        1 aatgcttttt cacttttctt cataaaaata atattcatcc tttatgagat ataatgaaaa
       61 aagcatatat aatagaaata taggcatttt cgtgatataa tttttaatat aaatttaggc
      121 tgctaaggat gagtagaaat gtcacgaatt acatgttttc ctgaatataa atccagaatt
      181 gtcggatatt tcaggatacg tacaattgaa tataaagatg tagatgcaaa tttactgttt
      241 aatgaatttg aaaaatgttc ctttgccatt ccggaattcc ctcccggtaa tctggatatg
      301 tggctacatt atcaggcaca tatattcctt atggacaaat tcaaaaacag cccggataaa
      361 tttcatctac tatacataga actaataaat aaatatggta tttttattaa agctgccgaa
      421 ttagaacact ttagggcata ttacccggaa gaactgtttt ttaatctgga ttttccgaca
      481 gatatgctaa ggaccttggt atgtaaagca agatatcctc ataggatcct gtgttactgc
      541 ttcagatatt tgctgcatgg ttggtccgct atcaggatag cttcagagct taacgattac
      601 gaacttgatg atatgctgga tttgtttctg aaagaataca aaaaggaatc gggattgcct
      661 tcattttttg tagactatat gttttcaccg ttaagaagga ttttgaacga ggttttggca
      721 aactacttat caagacaagg aaaaaccgac acaaaattca tagaagttat tcaacccata
      781 acaaaccaca gaatagggtc tacaaagctg gataattact ttggtgtaaa tgagctttcc
      841 cagagatcac acatggtttc ggtatggtgc aacaggatta aaaatactct gtactcaaat
      901 tatgtggctt tcttaaaaaa ttaattttta ttttatatgg tggtgtggta taaatgaatg
      961 ctgaaaaaac aaaatgtcct tcccatgaga ctataatgaa ttacttcaac atggagcttg
     1021 atgaaacaga agccataaaa gttgaagagc atttctcata ctgtaatgag tgcattggcc
     1081 agttcaatta ttataaagaa tatattgaac aaatcagtct ttcagcttcc gttcaaaaag
     1141 agctctggca caattctaat gtcgattttg tatcggtatc ggtggcagac tcatctcaag
     1201 taagagaagt atctgaattg gtaaccgata agggtaaata taccattagg cttattcctt
     1261 accaggatga aatgtcccgt gcccttttag taatagaggt tgccgatcga aacataaaag
     1321 gcgatatcaa ggtctttttg ttagacaagg caaatttgac ccatgtggga accgaaccgc
     1381 tcaacgagga acatcaggca tgttttgagg taccatccgg tattaatctt aaaaaactga
     1441 tattaaaggt agaaaatata taaaaattct tttcggcatg attccattat ggctattccc
     1501 aaggaaacac tgtgcttttt ttctttcaca tttttatctc ttacatagta tgcaaatttc
     1561 gaacatttgt ttgatttttc atttctaatg tgctataatt tacctataga atagcagtgc
     1621 acactacata ttttaggacg gtggatttat ggaacttcta aaaaagctgg aaattttatc
     1681 tgctgctgca aagtatgacg tgtcatgttc ttccagcgga agcaacagaa aaaacacaaa
     1741 aggcggaatc ggcaatgctt ctgcctgcgg tatatgccat agttgggctg acgacgggag
     1801 gtgtatctca ctactaaaaa tccttcttac aaattactgt atatatgact gtgcatactg
     1861 cgtaaacaga gtttctaatg atgttccccg tgccgcattc acacccaggg aagtcgccga
     1921 tctcacaata aatttttata aaaggaacta catagaaggg ctatttttaa gctcttccat
     1981 aattaaaaac ccaaaccaca caatggagct tttatacgaa tccgtcagga ttttgagaga
     2041 agaatattta tttaacggct atattcacat aaaagcaatt cccggagcag acctgaacct
     2101 tattgagtct acaggaagac ttgcagacag aataagtgta aacattgagc ttccctcaaa
     2161 taaaagcctt aagctcttag caccgcaaaa agaaaaggaa gcaataataa aacccatggg
     2221 ctttatcagt tcgaaaattg ttgagaaaaa ggaagaacga aggctgttga agaaagcacc
     2281 ggctttcgcc cctgcgggtc agagtactca gcttatagta ggagcaagcc ctgagcacga
     2341 tttaaatata ttaaggcttt cggaaaagct ctaccaaaac ttcaaactga aaagggtgta
     2401 ttactctgca tacgttccgg tttcatcaca aaacccaaat ctccctgcaa taaacacccc
     2461 tcctcttctg cgggaacaca gactttatca ggccgattgg ctcttgagat tttacggctt
     2521 cagttcggat gaattacttg atgaaaaaac accggacttt gacattcatc ttgatccgaa
     2581 agccaattgg gcccttaacc atatggagct ctttcctgtt gaaataaacc gggccgatta
     2641 tgaaatgctt ctaagagtcc cgggtatcgg ggtccgttca gccaacaaaa tcataagggc
     2701 aagaagggta aaatccttga atcatgacga tttaaaaaag ttggggatag tattgaagcg
     2761 agcccggttt tttattactt gcagcggaaa atactccggt gggatagaca tgaatgacac
     2821 cgttatccgg tacgcactta tggataaagc caacagattt gatttcactc ccacttggga
     2881 gcagctttcc ctcttttcac tcattgataa tatgcccaca gtaaatgata aaattatgag
     2941 tataacaggt gaaatgtaat gatatattac tattatgaca aaagctttga aggactcctt
     3001 acagcaatat atgaagccta ctataggaaa gaatcgccgt atatatgccc tgtacaaaaa
     3061 aacaatcaaa tgagtatttt cgatacgggt attaccattg aaacctccga agaaaaagcc
     3121 tccaaggtgt acgaggccat atataaaaaa atatcatata acgccctgaa aaatgcttac
     3181 catgcgcacc tttcggaaat tgacgggatc agtaatagca tatatcaata tattaagctg
     3241 gggtttaaag tgggaagaaa agtagatctt tatcttaccg atgagagagt ttttccggta
     3301 cataaagcag taaagtcggt tcaaagggaa agccacttga tgatgggtat aataagattt
     3361 aaaaaacttg ataatgatct gtattacgct tcctacgagc cggattacaa tattaccgtt
     3421 ctattaatac cccacttttc cgaaagattt tcgaatcaaa actggataat ccatgacata
     3481 aaaaggggca ttgctgcggt ttacgataaa aacgactgtg tcatcacgga tcttccggaa
     3541 gaaatatata aatgctccaa atcccggacc gaccaatatg aaactctttt taaaggtttt
     3601 tttaaaagca tatgcattaa tgaaaggatt aatccaaagc ttcaaaagca atacatgccc
     3661 aagagatatt ggaagcatct catcgaaaaa agcgattcat aaagcagctt
//
LOCUS       DAOOJC010000243         3676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_19486_length_3676_cov_3.703397, whole genome shotgun sequence.
ACCESSION   DAOOJC010000243 DAOOJC010000000
VERSION     DAOOJC010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19486_length_3676_cov_3.703397"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..2761
                     /gene="lon"
                     /locus_tag="PLA01_07140"
     CDS             314..2761
                     /gene="lon"
                     /locus_tag="PLA01_07140"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPD01111.1"
                     /translation="MSETKKIVKKQVLPLLPLRGLTVFPYMILHFDVGRVKSIKALEE
                     AMINNQLIFLVTQKDAKDDLPGIDDIYTIGTISRVKQLLKLPGDTIRVLVEGISRAEV
                     CEFTQIEPFFMAEVEERVFVEQEENKAEIEALTRRVLSVFEEYSKLNNRISPETVLSV
                     MNIEDPEQLADIITSNLMLKVEQKQEILSEFQPKLRLEKLLETLIREIDIMQIERDIN
                     IKVRKQIDKTQKEYYLREQLKAIQNELGDKEGITGEVEEYKRKLAEGNFGEEVEKKVL
                     KELDRLLKMPSGSAEGSVIRTYLDWIFDLPWNKKTEEIIDLNRSQEILDEDHYGLEKV
                     KERVIEYIAIRKLKNDLKGPILCLAGPPGVGKTSIAKSIARALNRNYVRMSLGGVRDE
                     AEIRGHRRTYVGAMPGRIISALKQAGSKNPLILLDEIDKMSSDFRGDPASAMLEVLDS
                     EQNFAFRDHYLELPFDLSDVLFITTANNLYTVPRPLLDRMEVIPVSSYTEEEKVNIAI
                     KYLFPKQIKEHGFKKSNLIIDEKAVRELINCYTRESGVRGLERQIASICRKVAKKLAS
                     TNQKTVKITAATLEKYLGAKKYKYDKANEKDEIGVATGLAWTPVGGDTLSIEVTLMDG
                     NGKLELTGQLGDVMKESARAAMSYIRSRAELFSIEKDFHSKYDIHIHVPEGAIPKDGP
                     SAGITLATAMVSALTDTPVRRNVAMTGEITLRGRVLPIGGLKEKVLAAHRAGIDTVII
                     PADNKKDLDDIPDNVRKKIKFVIAENMETVLDAALISKTNNNIEMGFGQTEENVIIHE
                     DASSINEVNSENLIIEQ"
     gene            3113..>3676
                     /locus_tag="PLA01_07145"
     CDS             3113..>3676
                     /locus_tag="PLA01_07145"
                     /inference="COORDINATES: protein motif:HMM:NF028468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HPD01112.1"
                     /translation="MKEIAKRLLKLKDYALIVATAIVAYSVFGFLGLFEEQFSQVMSA
                     ASFLAWHNLLEFTSVLISMTIFLVSYYTYNQTKKLRSICLGSVFLTIGILDAFHTLSY
                     QGMPAFLTENDDSNRATTLWIISRLIAGAGFLGVTFINTEIKSKLNKKIFLIVPAGFC
                     MLALVIVTYYPSFLPAMFIEGSGLTQWK"
BASE COUNT         1235 a          583 c          863 g          995 t
ORIGIN      
        1 tccaaataaa aataattgaa ataatgattt acaaaggtta taatgatggg gattcaagac
       61 tatattgtgt gtaaatctta cctttgtttt gcaattgttc atgagagatg ttaatgacgt
      121 caaaaaaagg gtagtattta atcatatgta ggaaatgaat tgtttacgca tgctgattat
      181 ttgggaaaag agaaatgtgc cttatatttg taattaacct tgattaacac aacacattct
      241 ttattgtttc tgttggaata ggcgtgaaag tcatagacga aagaacctca aattatagca
      301 aggggatgat caaatgtctg aaacaaagaa aattgtaaaa aagcaggttt tgccgttgtt
      361 acctcttaga ggtttaacag tatttcccta tatgattttg cattttgatg ttggcagggt
      421 taaatcgatt aaagccttag aagaagcaat gataaacaat cagctgattt ttttggttac
      481 tcaaaaggac gccaaggatg atttgcccgg aatagatgat atttatacaa tcggaacaat
      541 atccagagtt aagcagctct tgaagcttcc gggggataca ataagggttc tggtcgaggg
      601 tataagcagg gcagaagttt gtgagtttac acagattgag ccgtttttta tggcagaagt
      661 ggaagaaaga gtattcgttg agcaggaaga aaacaaggct gaaatagaag cattaaccag
      721 aagggtatta tcggtttttg aagaatactc gaagctcaat aacagaattt cgcctgaaac
      781 tgttttatcc gttatgaata ttgaagaccc ggaacaattg gcggatataa taacatcgaa
      841 tttgatgttg aaggtggaac agaagcagga gatattaagt gaatttcaac cgaaactgag
      901 acttgagaag cttcttgaaa ccctcataag agaaattgat atcatgcaga ttgaaagaga
      961 tatcaatatt aaggttagaa agcaaattga taagacacag aaggaatatt atttgagaga
     1021 acagctgaag gcaatacaga atgagctggg agacaaagaa ggtataacag gagaggtaga
     1081 ggaatataag agaaagcttg cggaaggcaa ttttggcgaa gaagttgaaa aaaaggtttt
     1141 aaaggaattg gaccgccttt taaaaatgcc ttcagggtct gcagaagggt cggtcataag
     1201 aacctacctt gactggatat ttgatttgcc ttggaacaag aaaacagaag aaattataga
     1261 tttgaatagg tcacaggaga ttcttgatga ggatcactat gggttggaaa aggttaaaga
     1321 aagggttatt gaatatattg ccataagaaa gttgaaaaat gaccttaaag gacctatttt
     1381 gtgcttagcg ggacctccgg gagtgggtaa gacatctata gcaaaatcca tagcccgtgc
     1441 cctcaaccgc aattacgtga gaatgtcatt gggaggagtc agggatgagg ctgaaataag
     1501 aggacaccgc agaacctatg taggtgctat gccgggtaga atcatatcag ctttgaagca
     1561 agccggctct aaaaatcctc tcatccttct cgatgagata gataaaatga gcagcgattt
     1621 tagaggtgat cccgcgtcgg ctatgcttga ggtattagac agcgagcaaa attttgcttt
     1681 tagagatcac tatttggagc ttccctttga tttgtctgat gttttgttta ttacaacggc
     1741 aaacaacctt tacaccgttc caaggcctct ccttgacagg atggaagtaa tacctgtttc
     1801 cagctatacc gaggaggaaa aggtcaacat agcgattaaa tatcttttcc ccaagcagat
     1861 caaggaacac ggatttaaga aaagcaacct gataattgat gagaaggctg tcagggaatt
     1921 aatcaactgc tatacgagag aatccggtgt aaggggattg gagaggcaga tagccagtat
     1981 atgcaggaaa gttgccaaaa agttggcttc gaccaaccag aagacggtaa aaattactgc
     2041 tgcaacactg gaaaagtatc ttggagccaa aaaatataaa tatgacaagg caaatgaaaa
     2101 ggatgagatc ggtgtggcta caggtcttgc atggacacct gtgggaggcg ataccttgtc
     2161 gattgaagta actttgatgg acggaaatgg gaaattggaa ctcaccgggc agcttggaga
     2221 tgtaatgaaa gaatctgcaa gggctgctat gagttatatc cgttcgagag ctgaactgtt
     2281 cagcattgaa aaggattttc acagcaagta tgatatccac attcacgtac ccgagggtgc
     2341 tattcccaag gatggacctt cagcgggaat tacccttgca actgcaatgg tatctgcttt
     2401 gaccgacaca cctgtcagga gaaatgtagc aatgacggga gagataacgt taaggggcag
     2461 agtgcttccg ataggcggac ttaaggaaaa ggtactggct gctcataggg caggaattga
     2521 tacagttatc ataccggctg acaataaaaa ggatcttgac gacattccgg ataatgtaag
     2581 gaagaagatt aaatttgtca ttgccgagaa tatggaaact gtgttggatg cggctttgat
     2641 aagtaagaca aataacaata ttgagatggg ctttgggcaa acggaagaga atgtaattat
     2701 acacgaagat gcctcttcta ttaatgaagt gaatagtgaa aaccttataa tagagcagta
     2761 gcttttcttg ataatcccac ggcgggttct caaactgcct atacaaggaa gttcttgatc
     2821 tttactattc aacaagcccg caggattttc atttaagcaa gtatccgaga gaacaaaaaa
     2881 ggaagcgaaa acagctgtgt tgaagaatgc ggctgctttt tattagtggg atttcacgaa
     2941 gtgaaatgac caaaaagcga tgggggctcg atgcccccgg agcttttatt aaaccaacat
     3001 tacctcatta taattgaata ttgaagtatc aaatggtaaa atgagaaaaa gaacgaatta
     3061 aaaatagagt aaaaagagag aaaaagctat gatgaatgct atggggatac gaatgaaaga
     3121 aattgcaaaa agactattaa agcttaaaga ttatgcacta attgtggcaa cggcaatagt
     3181 tgcctattcg gtttttggct ttttaggcct ttttgaagaa cagttcagcc aggtaatgag
     3241 tgctgccagc tttttagcat ggcacaacct tttagagttt accagtgtac tgatatccat
     3301 gacgatattt cttgtatcct attacaccta caatcaaaca aaaaaactgc gatccatttg
     3361 tcttggaagt gtgtttttga ccatcggcat tcttgatgct tttcatacat taagctacca
     3421 gggaatgccg gcatttctta ccgaaaatga cgattcaaac cgggccacaa ccctttggat
     3481 tatttccaga ttgatagccg gtgcaggatt tttgggagtt acctttatta atacggaaat
     3541 caaatcaaaa ttgaacaaaa aaatattttt gattgtaccg gcaggttttt gtatgctggc
     3601 tcttgttata gtcacttact atccgagttt tttgcctgcc atgtttatag agggcagtgg
     3661 acttacccaa tggaaa
//
LOCUS       DAOOJC010000244         3675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_19491_length_3675_cov_4.757182, whole genome shotgun sequence.
ACCESSION   DAOOJC010000244 DAOOJC010000000
VERSION     DAOOJC010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19491_length_3675_cov_4.757182"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..294
                     /locus_tag="PLA01_07150"
     tRNA            218..294
                     /locus_tag="PLA01_07150"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:252..254,aa:Met,seq:cat)
     gene            305..379
                     /locus_tag="PLA01_07155"
     tRNA            305..379
                     /locus_tag="PLA01_07155"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:338..340,aa:Glu,seq:ttc)
     gene            384..459
                     /locus_tag="PLA01_07160"
     tRNA            384..459
                     /locus_tag="PLA01_07160"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:417..419,aa:Val,seq:tac)
     gene            723..2042
                     /locus_tag="PLA01_07165"
     CDS             723..2042
                     /locus_tag="PLA01_07165"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPD01113.1"
                     /translation="MNMLKDKTKKSLFVLFLLIITILTGCGHDKATDDVIYEPGTNKV
                     ETETDSKAASNADTNIIDAFNDTTRDEESSEDKAAEITENPDYSEITISMIGDILLHT
                     KIHESGRMEDGSLNYDHLFANVKEDIEAVDLAIVNQEVILGGKELGLSGYPSFNGAFE
                     VGDALVKAGIDVVLHATNHTLDKGKKGVLNCMNYWKTAHPRIGVLGINDSREAQDNNI
                     YVYEKDDIKVAIMNYTYGTNGIPLPEDMPDIVNLLDKNKIERDVKRAKELADFVIVTP
                     HWGTEYKHIPSKSQRELAEFMAELGVDLVIGAHPHVIQPVEWIESDNGNKMLVFYSLG
                     NFINSTNETGDGIADRMVGAMAQVTIGKNEGEEALIKDYSAVPLVTHMKTGVGLITTY
                     KFEDYSAELAKENEIIKQDPNFSYEYCIDIWNETFPDFSYSGNGGSD"
     gene            2320..>3675
                     /locus_tag="PLA01_07170"
     CDS             2320..>3675
                     /locus_tag="PLA01_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HPD01114.1"
                     /translation="MKTQFIPYQKLSDLGKVYEKTKGGILGGLAVKKGNIVLLLITFL
                     LGRVSLFDNLMPFGMAFYAASAGIGTSRALMAAAVILGMITGGAVEELYAGLMFLAAF
                     TVIEWLFKNKAANNSVVHPVMGLVSIAVSQLIPVSMHGFLMYDVFRALLYTALVFSLI
                     FIFKRSIPFFNGTSSSRVLSSEETISAAILAAIALTGFGDMQIAGASLTNVFGILAVI
                     LFSFKAGPAVGSAVGVIVGLVITLSNPVTPLIIGTYGFCGLLSGVFRNLGKIGAGLGF
                     IIGNAMLTLYINGSTEVFIYIKDIIFASLIFTVIPQKIINNVMVAFSASPAVLKDKAC
                     YSERIKELTAERLNSFAKAFQELSKTFDEISQTQMVTGNQDISSLFDRVADKVCKDCS
                     LCLHCWDRNFYNTYQVMFKVVESLERKGWIEERDIPAYFMERCERIGEFVKQVNNIYE
                     LF"
BASE COUNT         1134 a          603 c          894 g         1044 t
ORIGIN      
        1 taagttactc ctatttacaa cattatacca taatatgaat attaactcta tatttttgta
       61 acgaggtgtt ataatactat ctctggagag gaaaaaccct gccaggaaat tacactggat
      121 ttttataaat tctacttgac tttttattta tgtattgcta caatagtaaa gcgtgctgga
      181 cattgttcga aaagaaggtg tcattgcacg aatatgtggc ggcgtagctc agttggcaag
      241 agcatacggt tcatacccgt agtgtcgttg gttcaaatcc gaccgccgct accattttaa
      301 tattggccca ttggtcaagt ggttaagaca tcgccctttc acggcggtaa caggggttcg
      361 agtcccctat gggtcaccaa taagggcgct tagctcagct gggagagcat ctgccttaca
      421 agcagggggt cataggttcg agccctatag tgcccaccac agcagttgta caatatggta
      481 cagctgcttt tttaattagt gggatttcgc agaagtgaaa tgtccaaaaa gcgaatgggc
      541 tcgatgccca tgagcttttg tgtcttaaga aactgcattg agtgagccgg taaccagcac
      601 tcactaattg aaaattttct ttgagcagaa gtgttataat agaagcagtg caagaggcag
      661 taaaaacggt ttatgatata aagaagatgt actataagaa caaagggtgg gattattaga
      721 aaatgaatat gcttaaggat aaaactaaga aaagtctttt tgtattattt ctccttatca
      781 ttactatcct tacgggctgt ggtcatgata aggcaacaga tgatgtcatt tatgaacccg
      841 gcacaaataa agttgagaca gaaacggaca gtaaagcagc aagcaatgca gatacgaata
      901 taattgatgc ttttaatgac acaacaagag atgaggaatc ttcggaagat aaagcagctg
      961 agataactga aaatccggac tatagcgaaa ttacaatatc aatgatagga gatattctgt
     1021 tacataccaa aattcacgaa agcggcagga tggaagatgg cagcctgaat tatgaccatt
     1081 tatttgcaaa tgtaaaagag gatatagaag cggtggatct tgcaatagtc aatcaggagg
     1141 taatactggg aggaaaggaa ctgggtttat ccggttatcc aagctttaac ggtgcttttg
     1201 aggtgggtga tgccttggta aaggctggca tcgatgtggt attgcatgct acaaaccaca
     1261 ctttggataa agggaaaaag ggtgttttaa actgtatgaa ttattggaaa acggctcatc
     1321 cccggattgg tgtcctggga attaacgata gccgggaagc acaggataat aatatctatg
     1381 tctatgaaaa agatgatatt aaagtggcga taatgaatta cacatacgga accaatggaa
     1441 ttcccttgcc cgaggatatg ccggatatcg ttaatcttct ggataaaaac aaaatagaaa
     1501 gggatgtaaa aagggcaaag gagctggcgg actttgttat tgttacacct cactggggaa
     1561 cggaatataa gcatataccg tcaaaaagcc aaagagagtt ggctgaattc atggcagaac
     1621 tgggagttga tttggtcatc ggggctcatc cccatgtaat tcagccggtg gaatggatag
     1681 agagtgataa cggtaataag atgctggtct tttattccct tggaaacttt attaattcaa
     1741 ccaatgagac cggggacgga atagctgaca ggatggtcgg agcaatggca caggttacta
     1801 taggaaaaaa tgagggagaa gaagcattga ttaaagatta tagtgcagtt cctctggtaa
     1861 cgcatatgaa aaccggtgtt ggccttatca ctacttataa atttgaagat tactcggctg
     1921 aattggcaaa agaaaacgag ataataaaac aagaccccaa tttttcttat gaatattgta
     1981 ttgatatttg gaacgagaca ttcccggatt tcagttattc cggcaatgga ggatccgatt
     2041 gaattaatat tttcttcata cgcagtaaaa agtttttgac gtaatactac aaaatagtcg
     2101 gatgtattta cggtttcgaa cttaaatgaa aacattgagt caatcttcct taaagttagt
     2161 ctgccatggt cggacgttgt gttttgggga taagtgtcac aataattttt gataggcaca
     2221 accaaaaccg acaaacattt ttatccccct gttttataat cggttctgca cggtaaaacc
     2281 gggcagattc taaaaattaa atttgggggg tcacaaaaga tgaagacaca atttattcct
     2341 tatcaaaaat taagcgattt gggaaaagtg tatgaaaaaa caaagggagg tattctgggg
     2401 ggactggcag tcaaaaaagg caatattgta ttgttgctta taaccttctt attgggaaga
     2461 gtatctttgt ttgacaacct tatgcccttc ggtatggcgt tttatgcggc ttcggcaggt
     2521 atcggcacaa gcagggcttt gatggcagca gcagtaatac tgggaatgat aacaggcggt
     2581 gcagtggaag aactgtatgc aggattgatg tttcttgcgg catttactgt aattgaatgg
     2641 ctgttcaaaa acaaagcggc gaataacagt gttgtgcatc cggttatggg actggtaagt
     2701 atagctgtct cccagttgat accggtgagc atgcatggat ttttgatgta tgacgttttc
     2761 agggccttat tgtacactgc actggtattt tcattgatat ttatttttaa aaggagcata
     2821 ccgtttttta acggaacaag cagtagccgt gtgctgtcca gtgaagaaac gataagtgcg
     2881 gcaatactgg ctgcaatcgc attgacaggc tttggagata tgcagatagc aggggcatct
     2941 ttgacaaacg tgtttggaat actggcagtg atactgttca gtttcaaggc gggaccggca
     3001 gtgggttcgg cggttggagt aattgtagga ttggttatta cactgtcaaa tcccgttaca
     3061 cctctaataa tcggtactta tgggttttgc gggcttttgt ccggtgtgtt cagaaacctt
     3121 ggaaagattg gtgccggtct gggttttatt attggaaacg caatgctgac tctctatata
     3181 aatggatcaa cggaagtgtt tatatatatt aaggacataa ttttcgcatc tttaattttt
     3241 accgttattc cgcaaaaaat tatcaacaat gttatggtag ctttttcagc cagtccggcg
     3301 gttttgaagg ataaagcctg ttacagtgag cgtattaaag agctgacagc ggaaaggtta
     3361 aatagttttg cgaaggcatt tcaggagtta tcaaagacct ttgacgaaat ttctcaaacc
     3421 caaatggtaa ccggcaatca ggacatttca tccctgtttg accgggtggc ggacaaggtg
     3481 tgcaaggatt gcagcttgtg ccttcattgc tgggacagga atttctacaa tacatatcag
     3541 gtaatgttta aggtggtgga aagccttgag agaaaaggct ggattgaaga aagggatata
     3601 ccggcatact ttatggaaag gtgcgagcgt ataggtgagt ttgtaaaaca ggtgaacaac
     3661 atttatgagc tgttc
//
LOCUS       DAOOJC010000245         3663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_19588_length_3663_cov_3.724501, whole genome shotgun sequence.
ACCESSION   DAOOJC010000245 DAOOJC010000000
VERSION     DAOOJC010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19588_length_3663_cov_3.724501"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3003
                     /locus_tag="PLA01_07175"
     CDS             <1..3003
                     /locus_tag="PLA01_07175"
                     /inference="COORDINATES: protein motif:HMM:NF019227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HPD01115.1"
                     /translation="DAMGRNTTIRDMLGNVTTFVYSNSINPYIMIDAKGREYKYTYDA
                     NGRRTMTTFPDGTTYCVSYDGAGNVISETDQAGNTTTYSYDYAGRLKSITDAMDNTWE
                     YEYDALGNLVKVTDPNLNETLYEYDELGRLIKRTLPMGMSETVEYDAEGNVIATTDFS
                     GNTIKYSYANGRLVEKKCPDGSTIRFEYYNGGLIKSVTDSSGTTTYQYNDTYGLISRT
                     NPDGSTVKYSYDKVGNRTEVITKAGTTKYVYDELNRVKKAISVDGGETVYTYDEVGNR
                     ISLTYPNGVKAKYTYDDLNRLVKVENIGSGGRILSSYEYTLGPSGNRTKVVESTGRTV
                     DYEYDELYRLVKETVTETDGSKRYISYTYDAVGNRLTKDDNGTKTVYTYDNNYRLIKE
                     GNINYSYDNNGNLVRKVEGQTTTQYEYDCDNRLVKVSTTNINGITTEEYLYDWQGNRI
                     QKRTNEVFVINYIVDSNRWLSRVIEERDGNGNLLVYYTIGDEEIISQTRGTKTSYYLY
                     DGHGSTRELTDEAGNITDTYPYDAFGNLLKKTGNTENSYLYAGEQFDPNTGFYYLRAR
                     YMDPAIGRFITMDSFAGSIYDPVSLHKYLYANASPVNFKDPTGYSSLSEMLNSINLQG
                     IIGSVQNTLLPLLKCLLKSAIKNAIRGAIRGAFYSSIDAALRGDSIIDGAINGAVYGA
                     LFGALATFDTIRDILMVVGLGTGAYGVKKALDEGNYLLAAWRAYSVYSALKAIKKGLF
                     DSKACFTEGTLILTADGYKEIQDIRVGDEVYSQNPKTGEKGLKRVINVFVREVDVLIH
                     IKAGEEDIETTKFHPFWIEGKGWVEAEDVKPGDRLVLYSGETLGVSEVYQEYLQSPVK
                     VYNFEVEDWHTYFVSEQDVFVHNAEGYTSSSNGSGNNGANGKGKNIVGTDFNEIKPTQ
                     TEINPQKVQSYADKLQAGENVKPIEVVNVNGKGKYIIEGHHRYVASQQTGIPVEVIET
                     QGDGPIGMPNWSLTEWLEWDGFDWIYNQ"
     gene            3080..>3663
                     /locus_tag="PLA01_07180"
     CDS             3080..>3663
                     /locus_tag="PLA01_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPD01116.1"
                     /translation="MSKDDIMDIHNHTNWSDGDSSIEDLVINALENNIGIIGISDHYN
                     NKHTRSIINFEDYILEIEILKNKYLSQIKILSGVEIPISSILCQEYSLKLINNLDFVL
                     VENIENLPPHFNLELLEEHLKNITCKKGLAHTNLQTIAAKYKDVGGINYFIEFMKKND
                     LFFEINSNSSFSFFDDFIYYHQNDWVKELFHNLNL"
BASE COUNT         1295 a          585 c          810 g          973 t
ORIGIN      
        1 gatgccatgg gacgcaatac cactatccgc gatatgttgg ggaatgttac aactttcgta
       61 tattcaaaca gtatcaatcc ctacataatg attgatgcca agggcaggga gtataaatat
      121 acttatgatg ccaacggcag aaggacaatg acaactttcc cggacggtac aacatactgt
      181 gtaagctatg acggagctgg aaatgtgatt tctgagaccg accaggccgg taataccacc
      241 acctattcgt atgattatgc cggaaggctt aagtcaataa ccgatgcgat ggacaacacc
      301 tgggaatatg aatatgatgc ccttgggaat ttggtaaagg taactgatcc gaatctcaat
      361 gaaactcttt atgagtatga tgagctcggc aggcttataa agaggacact tcccatggga
      421 atgtctgaaa ccgtagaata tgatgctgaa gggaatgtta ttgccacaac ggattttagc
      481 ggaaatacca taaaatattc ctatgcaaac ggaaggcttg tagaaaagaa atgtcccgac
      541 ggaagtacaa taagatttga atactataat ggaggactca ttaagagtgt aacggattcc
      601 agcggtacaa ccacctatca atataacgat acctatggac ttataagccg gacgaaccct
      661 gacggcagca ctgtaaaata ctcctatgac aaagtgggta accgtacgga agtcataaca
      721 aaagcaggaa caacaaagta tgtatacgat gaactgaaca gagtaaagaa agctatatct
      781 gtagacggag gagaaacggt ttatacttat gacgaagttg gaaacagaat cagccttaca
      841 tatcctaacg gggttaaagc taagtatacc tacgatgacc tcaacaggct ggtaaaggtt
      901 gaaaacatag gttccggcgg aaggatactg tcgagctatg aatacacttt aggaccttcc
      961 gggaacagga caaaggttgt tgagagtact ggaagaaccg tagattatga atatgacgaa
     1021 ttgtacaggc ttgtaaagga gaccgttacg gaaaccgacg gcagcaagcg gtatatttca
     1081 tatacttacg atgctgtagg aaacaggctt acaaaggacg ataacggaac aaaaaccgtc
     1141 tatacttatg acaacaacta caggcttatt aaagaaggaa atatcaatta ttcttatgac
     1201 aataacggaa accttgtaag aaaggttgaa ggtcaaacca ctacccaata cgaatatgat
     1261 tgtgacaacc gtcttgtaaa ggtaagcacc accaatataa acggtataac aacggaagaa
     1321 tatctgtatg actggcaagg aaacaggatt cagaagcgga caaacgaagt atttgtcatt
     1381 aattacatag tggacagcaa caggtggttg tccagagtta tagaagaaag agacggaaac
     1441 ggtaacctct tggtgtacta taccattgga gatgaagaga ttatatccca gacaaggggt
     1501 acaaaaacaa gctactactt gtatgacgga cacggaagca cccgggaatt gacggatgag
     1561 gcaggaaata taaccgatac gtatccctat gatgctttcg gcaatttgct gaaaaagacc
     1621 ggaaatacgg aaaacagtta tctctatgcg ggagaacagt ttgatcccaa taccggattc
     1681 tattacctga gggcaaggta catggatcct gcaatcggaa ggtttataac aatggattcc
     1741 tttgcaggaa gcatatatga tccggtgagt ctacataaat acctgtatgc caacgccagt
     1801 ccggttaact ttaaggatcc gacaggctat tcctctttat cggaaatgtt gaattccatt
     1861 aacctgcaag gtataatcgg ctcagtacaa aatacattac tgcctttatt aaaatgttta
     1921 ctgaagtctg ccataaaaaa tgcaatccgt ggtgcaatcc gtggagcatt ctatagttca
     1981 attgatgcgg cactaagggg agacagcatt atagacggag cgattaacgg agcagtatat
     2041 ggtgcacttt tcggtgcact ggcgaccttt gacacgataa gggatatttt gatggtagtc
     2101 gggctgggaa caggagcata tggtgttaag aaggcattag atgagggtaa ttacttactg
     2161 gctgcctgga gggcgtatag tgtttatagt gccttgaaag caataaaaaa aggattgttt
     2221 gattcaaagg catgttttac tgaaggaaca cttattctaa ctgcagatgg atacaaggag
     2281 atacaggata tacgggttgg agatgaggtt tattcacaaa acccaaagac cggggaaaag
     2341 ggactcaaga gagtaataaa tgtatttgtc agagaagtgg atgtccttat acacataaag
     2401 gcaggagaag aggatattga aaccaccaaa ttccatccgt tctggataga aggcaagggc
     2461 tgggtggaag cagaggatgt gaagccgggc gacagactcg tgttgtactc gggtgaaacc
     2521 cttggggtaa gtgaagtata tcaggagtat ttacagagcc ccgtaaaggt gtataatttt
     2581 gaagtagagg attggcatac gtattttgtt tcagagcagg atgtatttgt acataatgcc
     2641 gaaggatata ccagtagtag taatggatcc ggtaataatg gagcaaatgg aaaaggtaaa
     2701 aatattgttg gtactgattt caatgaaatt aaaccaactc aaactgaaat taatccacaa
     2761 aaagtacaaa gttacgcaga caaactacaa gcaggagaaa acgtaaaacc aattgaagta
     2821 gttaatgtta atggcaaagg aaaatatatt attgaagggc accatagata tgtagctagc
     2881 caacaaacgg gtattcctgt tgaagtaatt gaaactcaag gagatggtcc aataggtatg
     2941 ccaaattggt cgttaactga atggttagaa tgggatggat ttgattggat atataatcaa
     3001 taaataactc tagaacctta tttgacttag ctatatcaaa taaggttcta acttctaaaa
     3061 agaatggagt aattaattaa tgagtaaaga tgatattatg gatatacata atcatacaaa
     3121 ttggtctgac ggggattctt ctattgagga tttagtaatt aatgctctcg agaacaatat
     3181 tggaattata ggtatttctg accattataa taataaacac acacgttcaa taattaattt
     3241 tgaagattat attttagaga ttgaaatatt aaaaaataaa tatttatccc aaataaaaat
     3301 tctttctggt gttgaaatac ctatttcaag tatcctttgc caagaatatt ctttaaaact
     3361 aattaataat cttgattttg tacttgtgga aaatattgaa aatttacctc ctcattttaa
     3421 cttagagttg ttagaagaac atttaaaaaa tattacttgc aaaaaaggac ttgcacatac
     3481 aaatttacaa actatagctg cgaaatataa ggatgtaggt ggaattaatt atttcataga
     3541 atttatgaaa aaaaatgatt tgttttttga aatcaactct aattcaagtt ttagtttttt
     3601 tgatgatttt atttattatc atcaaaatga ttgggtgaaa gaattatttc ataacctgaa
     3661 tct
//
LOCUS       DAOOJC010000246         3661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_19600_length_3661_cov_3.332779, whole genome shotgun sequence.
ACCESSION   DAOOJC010000246 DAOOJC010000000
VERSION     DAOOJC010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19600_length_3661_cov_3.332779"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..408)
                     /locus_tag="PLA01_07185"
     CDS             complement(1..408)
                     /locus_tag="PLA01_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-associated protease 1"
                     /protein_id="HPD01117.1"
                     /translation="MPIKVTIAGGIVLEKCLDSVYFKVDTSDDLDARTTDIGNTIEIT
                     GRVDVDYEPTAGLYSWALIKPTSANKSDVYRKVEVEITSNKDNKLIRKLVFPEAFVVD
                     YLENYSNEDGLGSFTLFLKQKKDNNEVVNVSGS"
     gene            complement(964..1397)
                     /gene="rnpB"
                     /locus_tag="PLA01_07190"
     ncRNA           complement(964..1397)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLA01_07190"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1462..2565)
                     /locus_tag="PLA01_07195"
     CDS             complement(1462..2565)
                     /locus_tag="PLA01_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPD01118.1"
                     /translation="MSRISDIVRYMEELAPVNIAEDYDNVGLLIGNKGLEVNRILVCL
                     DVSSKVVDEAVEKNVDLIVSHHPVIFKGLKRINEDDAKGSIIYKLIKNNIGVYSAHTN
                     LDAALGGVNNYLSSLLGLTEISNLTGYKSEKLYKVVVFVPVDSVDPVRDSMSRAGAGW
                     IGNYSDCSFMAKGIGTFVPLERTNPYIGTRGKLEKVDEYRLETVVSKDNLQKVVEAMK
                     HAHPYEEVAYDVYPLEINGKEYGMGNVGVLDKPKTLEDFIALIKVRLNIKTVRLIGSI
                     DKEIKKVAVFCGSFDNKVAWAAKLQADVLVTGDVKYHDAMDMAEMGMCVIDAGHFNTE
                     RIIVPRLVQLLSNRFSDMEVMGNDVEEDPFKYC"
     gene            complement(2581..3285)
                     /locus_tag="PLA01_07200"
     CDS             complement(2581..3285)
                     /locus_tag="PLA01_07200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD01119.1"
                     /translation="MDLKGRLGLIADMVPQCDVVCDIGTDHAYIPIYLVMNNRCKRAV
                     ASDIRKGPIKVADKNIEKYNLRNYIDTRVGNGLETVRDGEEDTIIIAGMGGLIISGIL
                     EEGFETVKKANSLIIQPMYSIELVRKWLYENGFEIYDERLSKEDRRIYNVLAAKWTGS
                     VQKKEEIYYYIGEKLFENKDRLLESYIQNKLRQLNKIICEMEKMNDKNSALRLKNESI
                     REGLSRLLRDYHDERN"
     gene            3441..3629
                     /locus_tag="PLA01_07205"
     CDS             3441..3629
                     /locus_tag="PLA01_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01120.1"
                     /translation="MIQSFRVLNIKDTEHASETIISGISPSNITAAGTKNTAVVASSQ
                     QEITDNFDTFLENRVGGK"
BASE COUNT         1056 a          877 c          525 g         1203 t
ORIGIN      
        1 ttaagatcca cttacattaa caacttcatt gttatccttt ttttgtttta aaaataaggt
       61 gaagcttcct aagccatctt catttgagta attttcaaga taatcgacaa caaatgcctc
      121 aggaaaaaca agctttctta ttaatttatt gtctttattt gaagtaatct caacctcgac
      181 tttcctgtat acatcggatt tgttcgcact agtaggcttt atcaacgccc acgagtaaag
      241 tcctgctgtc ggttcgtagt caacatccac tcttccggtt atttctattg tattacctat
      301 atctgtagtc ctggcatcca aatcatctga ggtatccacc ttgaaatata ccgaatcgag
      361 acatttttca agaacaatac cgcctgcaat agtaacttta attggcataa tagctcctcc
      421 tttaatattc aatttaatat aaatgaatta gtattattaa cccgattaaa ttaaaggtgt
      481 gctcccccat ttcgcttaat tattatttga ttcaacaatt aacttattta ctctatatga
      541 atataattcc tgcaatcttt tttaccttta gtatatatca aatagaaaga aaacacaata
      601 tttgaaaact acattgtaca aattatctta gagttagaat tattttctac atgtattgtc
      661 catcatgcat tgtacatatt ggtgacctgt ttattgtttc atcaacttct cacctgctct
      721 tagtaaaaaa cgtattcata tggaattata acacatttca ggacctgaaa agtatataaa
      781 agcaaaaaaa taccattttt attctaaatg cagtacctat gtaacatgtt aattcttagc
      841 gattgaattc taatttatta cctgatttta acgtgataat ggaactgatc aaaacaaaaa
      901 caaagtaatg aaaacaagct gtatacacaa aaccacccgc cataaaagcg agtggtttaa
      961 taattagtga gtaagtctat aagccgggtt ctgtatttga tagtcatcta tctaggccat
     1021 atatttctat acagctcaag ccacctaccc gggactggcg ggccaccata acgtccctct
     1081 ttacggtgtt gctccaggtg gggtttacat agccggcaag tcgccatgcc gctggtgagc
     1141 tcttacctca cctttccacc cttgccgtgc tggagactgg aagtgagaag ttaaatataa
     1201 ttcaagcctc taacattaag cttctaattt ctatgctcta acgtcccact tccaacctcc
     1261 aacaggcggt atatctctgt tgcactttcc ttgaagtcgc cttcaccggg agttacccgg
     1321 caccctgccc tatggagccc ggactttcct cgtatacggt ctttcgacac tgtatccgcg
     1381 actatctggc ttactcatgt ttacttaatt cattcttatt atttacagca tattatatta
     1441 tatgtattta cagctctcat gtcaacaata tttaaatgga tcctcttcca catcgttccc
     1501 cattacctcc atgtctgaaa acctgtttga caaaagctgc acaagcctgg gcacaataat
     1561 tctttcggta ttgaaatgtc cagcatctat aacacacatg cccatctccg ccatatccat
     1621 ggcatcatga tatttcacat caccggtaac cagcacatct gcctgcaatt ttgccgccca
     1681 tgccactttg ttgtcaaagc ttccgcaaaa tacagccacc ttctttattt ccttgtctat
     1741 actgccgatt aacctgacgg tttttatatt gagacgcact tttattaaag caataaaatc
     1801 ctcaagggtt ttgggtttat ccaagacacc gacattcccc attccatatt ccttgccgtt
     1861 aatctccaag ggataaacat catatgccac ctcctcataa ggatgtgcat gtttcatagc
     1921 ttcaacaacc ttttggaggt tatcttttga aaccacagtt tccaacctat actcgtccac
     1981 cttttcaagc ttgccccttg tacctatgta tggatttgtc ctttcgaggg gtacaaaagt
     2041 accaatgcct tttgccataa acgaacaatc actgtaattg cctatccatc cggcaccggc
     2101 tctgctcatt gagtcccgta cagggtcaac actgtccaca ggaacaaaca caactacctt
     2161 atagagcttt tccgatttgt agccggtgag attgcttatt tctgtcaacc ccagtaaaga
     2221 agacagataa ttgtttactc ctcccaaagc agcatcaaga ttggtatggg cactgtaaac
     2281 cccgatatta ttctttatca gtttgtaaat aatgcttccc ttggcatcat cctcatttat
     2341 ccttttgagc cctttaaata ttacagggtg gtgggacaca atcaggtcaa cattcttttc
     2401 cacagcctcg tctacaacct ttgacgaaac atcaaggcac accaaaatgc gatttacctc
     2461 taatccctta tttccaatca gcaaacccac attatcataa tcctcggcaa tattgaccgg
     2521 agccagttcc tccatgtatc gtacaatatc gcttattctg ctcatccaaa cccctccttc
     2581 tcaattcctc tcatcatgat aatccctcag cagccttgaa agcccttctc ttatactctc
     2641 gttcttaagc cttaaagcag agtttttatc attcattttc tccatttcac atataatttt
     2701 attcaactgc cgcaatttat tttgaatata gctttcaaga agcctgtctt tattctcaaa
     2761 aagcttttca cctatataat aatatatttc ttccttcttt tgaacactgc ctgtccattt
     2821 tgcagccaat acattgtata tccttcggtc ttcctttgaa agcctctcgt catatatctc
     2881 aaagccgttt tcataaagcc atttgcgtac cagttctata gaatacatcg gttgaataat
     2941 aagggaattt gcttttttta cggtttcaaa cccctcttcc agtatcccgc taatgatcaa
     3001 accgcccatt cctgcaataa taatcgtatc ctcttctccg tcccttacag tttccaagcc
     3061 gttaccaacc cttgtgtcta tataattcct gaggttatac ttttctatgt ttttatctgc
     3121 cactttaata ggcccttttc taatatcact ggcaacagcc cttttacacc tgttattcat
     3181 aacgagataa ataggtatgt atgcatgatc cgttccaatg tcgcacacaa catcacattg
     3241 gggtaccata tcagcaataa gcccaagccg tccttttaaa tccatatttt tttcctttca
     3301 cttcggcatc attattaaaa tgggtaaata ttttctttac acttatctaa taaaccttta
     3361 tgaattatta tataaaaatt tcggagcata ataaacaatg taattatata aattgtagtg
     3421 tttataagga gatggcattt atgatacaga gctttcgggt tttgaacata aaagacactg
     3481 agcatgcttc agaaacaata atctccggaa tatctccctc aaacatcact gctgccggca
     3541 caaaaaacac cgctgtcgtt gcatccagtc agcaagaaat caccgacaac ttcgacacct
     3601 tcttagaaaa tcgggtagga ggtaaataat tattttattt accgacctcc cacaccaccg
     3661 t
//
LOCUS       DAOOJC010000247         3655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_19641_length_3655_cov_4.665833, whole genome shotgun sequence.
ACCESSION   DAOOJC010000247 DAOOJC010000000
VERSION     DAOOJC010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19641_length_3655_cov_4.665833"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /gene="rlmD"
                     /locus_tag="PLA01_07210"
     CDS             complement(<1..1200)
                     /gene="rlmD"
                     /locus_tag="PLA01_07210"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPD01121.1"
                     /translation="MTPVRKNEVYTMDITGMTHEGQGVGRIDNFTVFVDGALEGEKVE
                     LKIIKVNKSYGVGKLLKIIEVSKDRVAPFCASYQRCGGCSLQHMSYEAGLRFKTGLVK
                     ESIKRIGKLEDVVVHDAIGMEVPRYYRNKAQYPVGRDKDSVRVGFYAKRTHEIIDCPT
                     CAIQHSEGDKIKSIVKDFICSNGISTYDETTGEGLVRHIMTRFGFKTGEIMVVLVING
                     RSIPGQGKLVEKLVKEVPEIKSIVLNVNTKRTNVILGEENIVLYGKESISDYIGGISD
                     SEKSKKEGNSDGGFGFNISPQSFFQVNPVQTEVLYNKALEYAQLTGNETVFDLYCGIG
                     TISLFLSRKAKMVYGVEVVEEAIKDAKENARINGVENVEFIVGEAEEVIPQMYRKGIR
                     ADVVVVDP"
     gene            complement(1538..1870)
                     /locus_tag="PLA01_07215"
     CDS             complement(1538..1870)
                     /locus_tag="PLA01_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01122.1"
                     /translation="MFDNNGRTGLLVAGRTALSGWTDIEDAVTEARDKIRKSCAILGY
                     GSNNYTFYDLRDADPSNSEFPVSNDEIPIESRNTFFYPCFIDYDGKYLWVGDFKFSGG
                     IKRFPGKL"
     gene            complement(1974..2276)
                     /locus_tag="PLA01_07220"
     CDS             complement(1974..2276)
                     /locus_tag="PLA01_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01123.1"
                     /translation="MFDYCQYKYDFVYSYNNFGQPSGLFLTAPLKTDDGKVLEKGTLL
                     LREGASLSVWKDGKIDNENDRPDYYVGGGSKDDDRLHCFANSDSMPVLQDMNGNIY"
     gene            complement(2278..>3655)
                     /locus_tag="PLA01_07225"
     CDS             complement(2278..>3655)
                     /locus_tag="PLA01_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01124.1"
                     /translation="GGTIKGIMGNGITGGGLIYVNGGTVIGNFGSIEYEQGIVILNGG
                     DVKKVNYRSFSKEPDPENGYGVIRNGVFTGHAVLSRTILNVNDLTISEGSSMKVMRNG
                     GLIVKNSFVNKGTFTYESGLKSIGGEATVKPNPDGSHSPPKIIFDMSFNSLDIQENGH
                     LRIGNGATVTVTGAFSSGRNSSVVAENGTLRLLGSIDHKGRSEGIPELAALEDHGGGP
                     GPGGGRNYDREMDAANRLRDLGLFQGVGTHPDGGINFELERVPSRTEVLVMLIRLLGK
                     ENEALNNDWKHPFTDVPGWADKLVGYAYEKGLTKGVSPTEFGTGTASVQMYLTFVLRA
                     LGYSDGPGGDFTWDAPERFAQGVGILPGDVYMEDFRRADMVLISEAALFARLKDSDIT
                     LLDKLIEDGSVQKSDHEVTQPGNVAVIDSLDKASGVLTEGSSCQLMIKDANGADITWS
                     TSNRKYAG"
BASE COUNT          950 a          981 c          604 g         1120 t
ORIGIN      
        1 agggtcaacc acaacaacat ccgcccggat accctttcta tacatttgag gtatgacctc
       61 ttcagcttcg ccgactataa actccacatt ttccacacca ttaatccggg cattttcctt
      121 tgcatccttt attgcctctt caacaacctc cacaccataa accatttttg cctttctaga
      181 caaaaacaac gatatggtac ctatgccgca gtacaaatca aagactgtct cgtttcctgt
      241 aagctgtgca tactccaatg ccttattgta aagtacctct gtctgaaccg gattgacctg
      301 aaaaaatgat tgcggggata tgttgaaccc aaatccgcca tcggaattcc cttccttttt
      361 gctcttttca ctatctgaaa ttccgccaat ataatcggaa atgctttcct taccgtaaag
      421 gactatgttc tcttccccta aaattacatt ggttctcttt gtgtttacgt tcaatacaat
      481 gctttttatc tccggaactt ccttcaccaa tttctccaca agcttcccct gtcccggaat
      541 acttcttccg ttaataacca agaccaccat tatctctccg gttttaaatc cgaaacgggt
      601 cataatatgc ctcacaaggc cctcacctgt agtttcatca taagtactta tcccattgct
      661 gcaaataaag tctttaacta tagacttgat tttatcccct tcactgtgtt gtatagcaca
      721 tgtgggacaa tcaattattt cgtgggtcct ttttgcataa aaacccaccc taacggagtc
      781 cttatccctt cccaccgggt attgtgcctt gtttctatag tatcggggca cttccatacc
      841 aatggcatca tgcacaacta catcttccag cttccctatc ctctttatgc tctcttttac
      901 cagaccggtt ttaaacctca gtcccgcttc atagctcata tgctgcaaac tgcatccacc
      961 gcacctttgg tatgaagcac aaaagggagc aaccctgtct ttggaaacct ctataatttt
     1021 aagtaatttc cctaccccgt agcttttatt aacttttatt atctttaatt caaccttttc
     1081 tccttcaaga gcaccatcga caaataccgt aaagttatct atccttccaa ctccctgacc
     1141 ttcatgtgtc attccggtaa tatccatggt atatacttca tttttcctaa caggtgtcaa
     1201 agcatgtacc gcctttcatg cacttttgta tttatataaa cagctttaat tctcgttaag
     1261 cctgtcatct gtttgtttat tttctgctct ttttttaatc ttacttatca ttttttccaa
     1321 cagcttttat ttctattttc agcttaaaga attatcatgt caagcacaat atttatgtat
     1381 tcctttaatc ttttagcggc ccaaatcgat tttgtacaca tcaagcaaaa caaaagcgat
     1441 ggtagcatcg agcctctatt gctttttggt catttcactt cgtgaaatcc cgctaaaaaa
     1501 actgcagccc cataatataa gagctgcagg taaacttcta tagttttccc ggaaatcttt
     1561 ttattcctcc cgagaattta aaatcaccca cccaaaggta ttttccgtca taatcaataa
     1621 agcagggata aaagaatgta tttcttgatt ctatcggaat ttcatcattt gacacgggaa
     1681 actccgaatt ggaaggatcg gcgtccctca aatcatagaa tgtataattg ttacttccat
     1741 atccgagaat tgcacaagac tttctgattt tatcacgtgc ttccgttact gcatcttcaa
     1801 tatcagtcca gcccgataaa gcagttctgc ctgcaacaag caatcccgtc ctgccgttat
     1861 tatcaaacat tgtaatatcc tctacgggat agttgaaagt atagataaca tccggcggct
     1921 ctcccggaaa tgttcctgct ttatatcctt caattttacc tgaattagcc ccgctaatat
     1981 atatttccgt tcatatcctg caaaaccggc atagaatctg agtttgcgaa acaatgcagc
     2041 ctgtcatcat ctttactgcc accgcccaca taataatccg gtctgtcatt ttcattatct
     2101 attttaccgt ctttccaaac actcaaactt gctccctcac gcagcagcaa agttcccttt
     2161 tccaatactt ttccgtcatc ggttttcaac ggagctgtca ggaataatcc tgaaggctgt
     2221 ccgaaattat tatacgaata aacaaagtca tacttgtatt ggcagtagtc aaacatctta
     2281 tcctgcgtat ttcctgtttg acgtgctcca tgtaatatca gctccatttg cgtcttttat
     2341 cattaattgg caacttgaac cctccgttaa tactccggat gctttatcaa gggaatctat
     2401 aactgcgaca ttacctggct gcgttacctc atgatctgac ttttgtaccg aaccgtcttc
     2461 aatcaatttg tccagcagtg taatgtcact gtccttaagc ctggcaaaga gtgcggcttc
     2521 agaaatgagt accatatcgg cacggcggaa atcttccatg taaacatccc ccggcaggat
     2581 accaacacct tgtgcaaatc tttccggtgc gtcccatgtg aaatcaccgc cggggccatc
     2641 agagtagccc agtgcacgaa gaacaaaggt cagatacatt tgcacggagg ctgtgcctgt
     2701 accaaactcg gtgggggaga caccttttgt cagacctttt tcataagcat aacccacaag
     2761 cttatcagcc caccccggca catcggtaaa ggggtgtttc cagtcattgt tcagtgcctc
     2821 attctccttg cccaaaagac gtatcagcat aaccagcact tcggtacggg aaggaactcg
     2881 ctcaagttca aaattgatac ctccatccgg atgagtcccg acaccttgaa acaatcctag
     2941 atccctgagc ctattggcgg catccatttc cctgtcataa ttacgcccac cccccggtcc
     3001 cgggcctccg ccgtgatctt ccagtgccgc aagttccggt ataccctcag accggccttt
     3061 atggtctata ctgcctaaaa gccgcagtgt tccgttttca gccacaacac tgctgttcct
     3121 gccggatgaa aaggcaccgg tgacagtcac agttgcaccg ttcccaatgc gtaggtggcc
     3181 attttcctga atatccaggc tgttaaagct catatcaaag attattttcg gcggtgaatg
     3241 agatccatcc ggattcggct tgaccgtcgc ctcgccgcca atgcttttca gacccgattc
     3301 atatgtaaat gtgcctttgt tgacaaaact gttttttacg ataagaccac cgttcctcat
     3361 caccttcatg gacgagccct cgctgatggt aagatcgttg acattcagaa tagtacgact
     3421 taaaaccgca tggccggtga atacaccgtt cctgattaca ccatatccat tctccggatc
     3481 aggttctttg ctaaaagaac gataattcac tttttttaca tccccgccgt tcagtataac
     3541 aattccttgc tcgtattcaa tgctgccgaa attgccgatc accgtcccac cattgacata
     3601 aataagaccg ccgcctgtga tgccattgcc catgataccc ttaatcgtgc cgccg
//
LOCUS       DAOOJC010000248         3645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_19719_length_3645_cov_2.945404, whole genome shotgun sequence.
ACCESSION   DAOOJC010000248 DAOOJC010000000
VERSION     DAOOJC010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19719_length_3645_cov_2.945404"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..1257
                     /gene="gpr"
                     /locus_tag="PLA01_07230"
     CDS             259..1257
                     /gene="gpr"
                     /locus_tag="PLA01_07230"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HPD01125.1"
                     /translation="MERSVRTDLVMEAHEILKENELRQGVDDRKDPPGVDVENAGTDD
                     IKITRVRVTSPTGEAAIGKPMGNYITLEVPRLKENDQVLYEETCRALAKELTGILELK
                     ERSTILVVGLGNWNVTPDALGPKVISGLMITRHLHEYVPEQIDKGVRPVCAIAPGVLG
                     ITGIETGEIVKGIVDRVKPEFVVAIDALASRKMERVNTTIQIADTGISPGSGVGNKRM
                     ELSKTTLGVPVIAIGVPTVVDAATMANDTIDLVIDNLIKEAKEDVAFYNMLKNIDRNE
                     KYQLIQEVLQPYVGNLVVTPKEIDDAVDRISKVIANGLNIALHQGITLNDVNRYLQ"
     gene            1560..2396
                     /locus_tag="PLA01_07235"
     CDS             1560..2396
                     /locus_tag="PLA01_07235"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPD01126.1"
                     /translation="MGLKSFMGKILRLPPRTWIMIFILAVAVAIGIGVYSDRTKNEPR
                     DNSHLTDNKNKTGNDGASVSDGTGPKEEKKPKEVIVVIDPGHGGEDLGAYNGNLLEKD
                     INLDISLMLGEILDELGINVVYTRETDVFIDLIPRSDIANELDATLFISVHSNAMEDY
                     PDYKGTETLYCPPPQNPRYTKMDGKKLAKIVQRELVKALGTADNGIIERPNLSVLRRT
                     KMPAIIAEIAYLSNPSDRAKLADASFRKKAAKALADAVVKALNEMGAVKDEKGVFMIM
                     EE"
     gene            2633..3328
                     /locus_tag="PLA01_07240"
     CDS             2633..3328
                     /locus_tag="PLA01_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HPD01127.1"
                     /translation="MRGLWRYLFILVITVLLWNQAVLRPVKMLSVLFHKMGHALAAFF
                     CGYGTGAFRTVFGGLGDITVSTKDWLPSFIITTGGYIASALFFVIIMGLKRTKAKKYV
                     PGCLSIIYLFVTIINPGLRQAAIYAAIFTSIVIVLSMIQREDIEELVLDVIGVSAVAY
                     IIFDTFVATVLLKINQQFSIIRGWNLRVPEDIVGLSNATGIPQVLLAVIWLILSVLVL
                     NLVILKSLKLKRR"
     gene            3401..>3645
                     /locus_tag="PLA01_07245"
     CDS             3401..>3645
                     /locus_tag="PLA01_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPD01128.1"
                     /translation="MKIEKRICELREILNYHSHKYYVEDAPEISDYEYDALYRELEKL
                     EEMRPDLITPDSPTQRVGDKPLEGFEKVIHAVQMQSLA"
BASE COUNT         1204 a          524 c          860 g         1057 t
ORIGIN      
        1 acctcctgcg agttatttaa caaaaagtat tttaacatga accaaactaa attgcaaggg
       61 ttaaatcata tttttcccac aaacagaggt gtatattatt taaatgaaga aaatgcggag
      121 gaaacactgc ggtatttaag tttcccgtat attttccctc agatttgagt aattgagctc
      181 aaaaatagag aaatgaataa acaatattaa taagatagaa aataataaaa actataagaa
      241 aagaaagggg ctttttaagt ggagagaagc gtaagaacag accttgtaat ggaggcacat
      301 gagatattaa aagaaaatga actgaggcag ggagtagatg acaggaagga cccacccgga
      361 gttgatgttg aaaatgccgg aaccgatgat attaagatta ccagggttag ggttacgtca
      421 cctacgggag aagcggctat aggcaagcct atgggtaatt acattaccct tgaggttcca
      481 agacttaagg agaatgacca ggttttgtat gaagaaacct gcagagccct ggcaaaagaa
      541 ttaaccggta ttctggaact caaagaaagg tcgacaattc tggtggtggg actgggaaac
      601 tggaatgtca caccggatgc attagggccg aaagttatat ccgggcttat gattacaaga
      661 catttgcatg aatacgtgcc tgaacagatt gacaagggtg ttcgtccggt ttgtgcaatt
      721 gctcccggtg tgctggggat aacgggtatc gaaacaggtg aaattgttaa aggaattgtg
      781 gacagggtta agcctgagtt tgtagttgcg attgatgctc tggcttcaag aaagatggaa
      841 agggtgaata ctaccattca gattgcagat acgggaatat cgcccgggtc aggggtaggc
      901 aataaaagga tggagctttc aaagactact cttggtgtac cggttattgc aattggagtt
      961 cctacggttg tagatgcggc aactatggca aatgacacca ttgatcttgt tattgacaac
     1021 ttaataaaag aagcaaaaga agatgtggcc ttttacaata tgttgaagaa tattgacaga
     1081 aacgaaaaat accaattgat acaggaagtg cttcagccat acgttggaaa tcttgtagta
     1141 acccctaagg agattgacga tgccgttgac aggatttcca aggtaatagc aaacggtctt
     1201 aacattgccc ttcaccaagg tataacatta aacgatgtca acagatatct gcagtgatta
     1261 tgcatattgc accaattgat taaaatgcgg gaaaacttta tgaagcttag tcgcaagctg
     1321 tttttatatt atattaccat aatgtgaaaa cagcttttat taaatttatg ttttataaat
     1381 catttctagt tgaattaact acaggctagt agtatgattt tattaggaca gttttttatg
     1441 ttgaaatgac aaaaagtaca aagatttggt gccttccgat tttttacata aaattaaagt
     1501 gacagttaag atgcttcatt ctttatatgt ttataataca gatcaaaatg gggaattata
     1561 tgggtttgaa gagttttatg gggaaaattt taagactccc tccgagaaca tggataatga
     1621 tttttatttt ggctgtggcg gttgcaatcg gcattggagt atatagtgac agaacgaaaa
     1681 atgagccccg tgataacagc catttaacgg ataacaaaaa taaaaccggc aatgatggtg
     1741 cttcagtttc agatgggaca ggcccaaagg aagagaaaaa gccgaaagaa gtaattgttg
     1801 tgattgatcc gggtcatggc ggagaggatt tgggggcgta taacgggaac cttcttgaaa
     1861 aagatattaa tcttgacata tcattgatgc ttggtgaaat ccttgatgag ttggggatca
     1921 atgtggtgta tacaagagaa acggatgttt ttattgacct tataccaagg tccgacattg
     1981 caaatgaact ggatgcgacc ctttttatca gtgtccacag taatgcaatg gaggattatc
     2041 cggattataa aggtacggaa actctttatt gtcctcctcc acaaaatccc aggtacacta
     2101 aaatggatgg aaaaaagctt gcaaaaattg ttcagcggga attggtaaag gccttgggaa
     2161 ctgcggataa tgggataatt gaacgtccga acctttcagt tttgagaaga acgaaaatgc
     2221 cggcaataat agctgagatt gcgtatttgt caaatccgtc ggatagagca aaactggcgg
     2281 atgcatcctt tagaaaaaaa gctgccaagg ctcttgcaga tgctgtagtt aaggccctta
     2341 atgaaatggg tgcggtgaaa gatgaaaagg gagtattcat gataatggaa gaataatgta
     2401 agggattatg ttcacaatca taatatttcc gttagaaccg gtacatttaa aaagaatgca
     2461 attatatagc taagaacgta aaatacatgt ctcaaggcaa aatggtaatt gagattacag
     2521 tggtataaaa taaaaaaaga gggaaatctc tttttttttt cgtcatttaa aggtataatt
     2581 ttaatatagg ggatattttg gtttttttta tttatagatt ataggggtag atatgagagg
     2641 gttatggaga tatctattta ttcttgttat tacagtctta ttgtggaatc aagcagtatt
     2701 aagaccggta aaaatgcttt cggtattatt ccataagatg ggacatgctt tggctgcttt
     2761 cttttgcggt tatggaactg gtgctttcag aactgtgttt ggaggactgg gcgacataac
     2821 agtaagtaca aaagactggc tgccgtcatt tataataacg actggcggtt acatagcgag
     2881 tgcattgttt tttgtaatta taatgggttt aaaaaggaca aaggctaaaa agtatgtacc
     2941 ggggtgtttg tctataatat atttatttgt cacaataatc aatcctggct tgaggcaagc
     3001 tgcaatatac gctgcaatct ttacatcaat tgtcattgtt ttatccatga tacaaaggga
     3061 ggacatagaa gaattggtat tggatgtaat aggtgtatcc gctgttgcat atattatatt
     3121 cgatacattt gttgcgacag ttttattaaa gattaaccag cagttttcaa tcatcagggg
     3181 atggaattta agagttcccg aggatatagt cgggctgagc aatgcgacag gtatcccaca
     3241 agtgttgttg gctgttatat ggcttatatt gtcagtgctt gtattgaatt tggtgatact
     3301 taaaagtttg aagctaaaga gaagataaga ggtttggtat taattttaat ttaatcacga
     3361 tactaaacat tttgcatata tacgacggag ggaatagtgg gtgaagattg aaaagaggat
     3421 atgcgagctg agggagattt tgaattacca cagccacaag tattatgttg aagatgcacc
     3481 ggaaataagt gattatgagt atgatgcttt atacagagaa cttgagaagc ttgaggagat
     3541 gagaccggac cttataacac cggattctcc gactcaaaga gttggggata aaccccttga
     3601 gggatttgaa aaggttatac atgcggtaca gatgcaaagt cttgc
//
LOCUS       DAOOJC010000249         3624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_19861_length_3624_cov_3.745867, whole genome shotgun sequence.
ACCESSION   DAOOJC010000249 DAOOJC010000000
VERSION     DAOOJC010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19861_length_3624_cov_3.745867"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLA01_07250"
     CDS             complement(<1..274)
                     /locus_tag="PLA01_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01129.1"
                     /translation="MNNFNTNPYINYYGNYTDNRFNYANRQNTYTLDRQIGDVNGDGV
                     PDIVYLVGEKSQNNFYENIRVMVQDGSTLNWYVIPLYSNYSMAFDPW"
     gene            complement(392..694)
                     /locus_tag="PLA01_07255"
                     /pseudo
     CDS             complement(392..694)
                     /locus_tag="PLA01_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
     gene            1123..2400
                     /gene="serS"
                     /locus_tag="PLA01_07260"
     CDS             1123..2400
                     /gene="serS"
                     /locus_tag="PLA01_07260"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPD01130.1"
                     /translation="MLDIKFVRENPEIVKQNIRNKFQDNKLGLVDEVIALDAELRSVK
                     QKADSLRAERNRISKQIGGLLARGEKEKAEEMKKQVNADSEQLAELKTKEDELENKVI
                     NILMTIPNIIDPSVPIGKDDSENVELERFGEPKVPDFEIPYHTEIMEKLSGIDLDSAR
                     KVAGNGFYYLMGNIARLHSAVISYARDFMIDRGFTYCIPPFMIRSQVVTGVMSFAEMD
                     AMMYKIECEDLYLIGTSEHSMIGKFIDTILPESSLPQTLTSYSPCFRKEKGAHGLEER
                     GVYRIHQFEKQEMIVVCNPEDSMMWYNKLWKNTVDLFRSLDIPVRTLECCSGDLADLK
                     VKSVDIEAWSPRQKKYFEVGSCSNLGDAQARRLKIRVDGENGKYFPHTLNNTVVAPPR
                     MLIAFLENNLNEDGTVNIPDVLRPYMGGMSVIK"
     gene            complement(2471..3313)
                     /locus_tag="PLA01_07265"
     CDS             complement(2471..3313)
                     /locus_tag="PLA01_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPD01131.1"
                     /translation="MERKISQQVRGYRTKDGAGVSLVRVLGDQTVQKYDPILLLDSFD
                     STNPEDYTAGFPMHPHRGIETISYVYRGFMTHKDSLGNEDTIGDGEVQWMTAGSGIMH
                     EEKLPAAERLLGVQLWLNLPAKDKMAPPAYRSIKNSEIEEIDLDCGKLRLLAGQFKER
                     KGYMSKYLPLDYYDIHLNPNASMLLSTDSERSVMIFTLLGDAYIGGELVKEKTAVKLT
                     SGDQVEIKSTDKNAQVLFISSVFLNEPVVWGGPIVMNTKEELNKAFDDLRKGTFIQKT
                     ISYL"
BASE COUNT         1106 a          721 c          674 g         1123 t
ORIGIN      
        1 gccatggatc gaaagccata ctgtaattgg aatacagagg tataacatac caattcagag
       61 tactgccgtc ctgtaccatt actctaatgt tttcataaaa attattctga cttttttcac
      121 ccaccagata aactatatcc ggtaccccgt caccatttac atcccctatt tgtctgtcaa
      181 gggtgtatgt attttgtctg ttggcataat taaacctatt atctgtataa ttcccataat
      241 aattaatgta cggattggta ttgaagttgt tcatgttgac ctcccatata tactttttaa
      301 tatattatat gaaggtcatc gctttaagga accagataat aggtttcatt cgtatcttac
      361 gtattagaaa ttgttctttt aatttttctt attataactc agccaacatc ttctccggat
      421 catctgcagc ctttacctta tcatttgcct taacaataat gccctcttca tctataagat
      481 atgtggttct gacaaccccc atagacactc taccgcattg catcttttcc ttccacacat
      541 cataagcctc tatgacctta cgttccgggt ccgaaagaag tgtaaacgcc aatccgtatt
      601 tttcttcgaa tttcttgtgg gaatcaaccg aatccttgct cacaccaaga actaccgcac
      661 ccttttccat aaactgcggg tacctttccg aataagaata taccacacaa cccaagaata
      721 tatttaaaaa atttatagcg gtgcataata tattttcaca ctctcgaact gataaatcat
      781 ctggtctgcg ttcctcataa gccccttaac cgctttatac cttactgcaa caacaggcaa
      841 tgcataggca gcccgaatta cagatttctc ttgttttctg gtattattat acttgaaagt
      901 taatataaaa cgaaatataa ttaatacaca acttagaggt tagtaagctt tatcaaactg
      961 atgcggagtg actggtagat tttttgaccc gaagttttgg cggcaacggg aattactccg
     1021 ttctctatag atatctaata attatactca gatatcttat acagaattat attttttaaa
     1081 tttgtgatta taaataataa tagctgatcg ggaggaataa acatgttgga tataaaattt
     1141 gtaagagaga accctgaaat tgtcaagcaa aacataagaa ataagtttca ggataataag
     1201 ctgggtttgg tggatgaagt tatagcactg gatgcagaac tcagaagtgt aaaacagaag
     1261 gcagattcat taagagccga aagaaatagg atttcaaagc agatcggcgg attgcttgcc
     1321 cgaggagaaa aggaaaaagc agaagagatg aagaaacagg ttaatgcaga ttccgagcaa
     1381 ttagcagagt tgaaaacgaa agaagatgaa ttagaaaata aagttattaa tattcttatg
     1441 accattccca acataattga ccccagtgta cccatcggca aggatgacag cgaaaatgtg
     1501 gaactggagc gtttcggtga gccgaaggta cccgattttg aaattccata tcatactgaa
     1561 attatggaaa aactgagcgg cattgatttg gatagtgcca gaaaggttgc tggaaatggt
     1621 ttttattact tgatgggtaa catcgccagg cttcattctg cagtgatttc ctatgcaaga
     1681 gattttatga tagaccgggg cttcacttat tgtattcctc catttatgat tcgcagccag
     1741 gtggtaaccg gagttatgag ttttgcagaa atggatgcaa tgatgtacaa aatagagtgt
     1801 gaagatttat atttgattgg aaccagtgag cattctatga tcggtaagtt tatagataca
     1861 atattgccgg aatcctcttt gcctcaaact ttgaccagtt attcgccttg ctttagaaaa
     1921 gaaaaagggg ctcatggtct ggaagaaaga ggagtatata gaattcacca atttgaaaag
     1981 caagaaatga tagttgtttg taaccctgaa gacagtatga tgtggtacaa taagctatgg
     2041 aaaaacacag tagatttatt ccgctctttg gatatacctg tgagaacttt agagtgctgt
     2101 tccggtgacc ttgcagattt aaaggtaaaa tcagtggata tagaagcatg gtctcccaga
     2161 cagaaaaaat actttgaagt gggaagctgt tcaaacttgg gtgatgctca agcccgtcgc
     2221 cttaaaatcc gtgtagatgg tgaaaatggc aagtacttcc cgcatacact aaacaatact
     2281 gttgttgctc caccaagaat gttgattgcc tttttagaaa acaatttaaa tgaggatggt
     2341 actgtaaata ttccagatgt attgaggcca tatatgggcg gtatgtcggt gattaaatag
     2401 aatcatatta tttttatata agaaagggcg ttgaacatac aaaaatcaac gcctttttta
     2461 cgtgttatat ttataagtaa gatattgtct tctgaataaa tgtgcctttt ctcaaatcat
     2521 caaaagcttt attcaattct tccttagtgt tcattacaat aggaccgccc caaactaccg
     2581 gttcgttcaa aaataccgaa ctgataaata gtacttgggc atttttatct gtagacttta
     2641 tctccacttg atcaccagag gtaagtttta ccgccgtctt ttcctttacc agttctcccc
     2701 cgatataagc gtcgcctaaa agcgtaaata tcattactga acgctcgcta tcagtactca
     2761 agagcataga agcattagga ttaaggtgaa tatcatagta gtccaggggc aaatatttgc
     2821 tcatatatcc ttttctctct ttaaattgcc ctgctaataa tcttaattta ccgcaatcaa
     2881 gatctatttc ctcaatttct gagtttttga tgctgcgata agcgggtgga gccattttgt
     2941 cctttgcggg aaggttaagc catagctgca ctcccaatag tctttcggcc gccgggagtt
     3001 tttcttcatg cataataccg gaaccggctg tcatccattg gacctctcca tcaccaatcg
     3061 tatcctcatt tcctaaactg tccttatgag tcataaatcc gcgatacaca taactgattg
     3121 tctcgatgcc tctgtgggga tgcattggaa accccgccgt gtaatcctcc ggattggtgc
     3181 tgtcaaagga atcgagcagt aaaattggat catatttctg aacagtttgg tctcccaaca
     3241 ctctgaccaa acttactccg gctccgtcct tagttctata gcctctaact tgttgactga
     3301 tttttcgttc catatagcca gcctccttat ttaaaactta ctgcacatat gttactcagt
     3361 ttttgcagca aaatacggct ttctgatatt cttctactca tgatagagca gagcagtgtc
     3421 tcaacgtaag ttgaatttaa acgtatcacc atcaaaagct ccaatagtta tatatagtgt
     3481 tcttacatat gtttgctgac ctgcccccct aaaaaatcaa agaaacagcc tctgaccttc
     3541 ggacattgga tccaatgtcc agaggcagca accttttgtt acacgaaacc ccaggttctc
     3601 tcccgattct tcgagatatt tgcg
//
LOCUS       DAOOJC010000250         3622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_19881_length_3622_cov_4.258200, whole genome shotgun sequence.
ACCESSION   DAOOJC010000250 DAOOJC010000000
VERSION     DAOOJC010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19881_length_3622_cov_4.258200"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLA01_07270"
     CDS             complement(<1..264)
                     /locus_tag="PLA01_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624050.1"
                     /note="catalyzes the formation of citrate from acetyl-CoA
                     and oxaloacetate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HPD01132.1"
                     /translation="MKNGNLTEFEGKMLDELSNLASKSSRIDPELFTKYEVKRGLRDL
                     DGKGVLVGLTEIGEVHSYIIDENEIIPVPGRLIYRGIDIFDLVD"
     gene            complement(404..1492)
                     /locus_tag="PLA01_07275"
     CDS             complement(404..1492)
                     /locus_tag="PLA01_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HPD01133.1"
                     /translation="MLYDKDKISIRQAMFLFLTVIASPAIRLVPVIAAQTAQEAAWLA
                     PIVAAVILFLNVLVWNRIYKKYKNDSLMHIYSDIAGKFIGKLLSIIYFLWATLLVCVY
                     VMYFAVRLVGSIYPDTNPSIFIISILVLVVYLLRYGLVTLARLNEIVFPMFAVTFLII
                     FIMLMPYLQVIFLGPVTYKSILPVINAGIGISGLSSYYTFLFVLGDKINNKESIKKIG
                     IQTTIFILVNQILLIVMTLGSFSYSVIKRSQLPLLLAVKQISLFNTLEKIESVIVAGW
                     VFSDFVLICFFSIIALKILKSLFGLSDERHLAKIFMVLIFFLSLYLSSTIFELERLSS
                     YVLIPGDFILGFIVPVIMLAVGKIRKKI"
     gene            complement(1506..1706)
                     /locus_tag="PLA01_07280"
     CDS             complement(1506..1706)
                     /locus_tag="PLA01_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01134.1"
                     /translation="MIKLVIFVVILSFIEIKSMVKKQQKKEIFVYLGLAVISLALGLL
                     YLSNPYRTSLAQHMLRLIGQEF"
     gene            complement(1703..2896)
                     /locus_tag="PLA01_07285"
     CDS             complement(1703..2896)
                     /locus_tag="PLA01_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HPD01135.1"
                     /translation="MRRIISLLLLFIVTLTLTACENPIMSRHEINDLEFARSIAIDTA
                     KSIPDGVRLTLASQRIKIGSGGSVGEKESSKISSEGRTMFEAVRNFSTYLGKRPFWGH
                     LEFVLIGEEAAKKGIHNYLDFFARDHEVRMNINVFVMKGCCAEELMEKADRGDRLIFD
                     RLNGLLNSLGGQSVSNPVNLIEVMYIQDMKCLSLYIPCVEMVNVTKGQDEDVIDVKMA
                     GFAIFDEDKLAGYLDENMGRGLNLLRNKVKSGIYVVNTRKGSKVSLEIINSKTKFYPE
                     IKDGNLSLKVKVNMESNIGEIDSNEDIFNNDTFIYLEQEQSRLVKQEIERVIEYAQKE
                     IGLDFFSTALNFYRKYPIKWKKLYEKDWKTWFPKIKFSVEVKSKIRRTYDIREPNAVE
                     GEGGK"
     gene            complement(2908..>3622)
                     /locus_tag="PLA01_07290"
     CDS             complement(2908..>3622)
                     /locus_tag="PLA01_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HPD01136.1"
                     /translation="IIDGLPTTFVAPGTLSQFLQGPKDYSQNFISSSIFRFMRYVLMV
                     VTLFLPGFYIAITSFHHEMIPTELALSITASKEGVPFPGFIEVIFMLIAFEALLEAGL
                     RLPRNISQAVTIVGALVVGQAAVEARLVSPAVVVIISITAVASFTMPDQDFSNALRIW
                     RFGLVILTSFAGLYGLSMGGLILLIHWSKMESFGVPYLAPFVATDDKQLQDTLIRMPF
                     STMYKRPIQLKTTNKKRRS"
BASE COUNT         1168 a          812 c          546 g         1096 t
ORIGIN      
        1 gtcaaccaga tcaaaaatat ctatacctct gtagatcaac cttcccggaa ccggtataat
       61 ctcattctca tcaatgatat atgaatgaac ctcaccgatc tcagtcaaac ctacaagtac
      121 acccttaccg tctaaatcac gcaaacccct cttaacttca tactttgtaa aaagctccgg
      181 atcgattcta ctactcttag atgcaagatt actcaattca tcaagcattt ttccttcaaa
      241 ttcagttaaa ttaccattct tcatagtcct atacctcaca tttataatat atgggttagt
      301 ttgctgatta ataacataag cataataagt aaatttattt tatcatatga cttataatta
      361 gtctatattt atttcaatta gggcattaga gcaatatttt tcgtcatatc ttctttcgaa
      421 ttttgccgac ggcaagcatt atgaccggta cgataaaacc taaaataaaa tctccgggaa
      481 ttaacacata gcttgacaac ctttccaact cgaaaatcgt gctggatagg tacaatgaca
      541 ggaagaaaat taaaaccatg aatattttag caagatgtct ttcatcggaa agcccgaaca
      601 atgacttcaa aattttcaat gcaataatag aaaaaaagca tatcaatacg aaatcggaaa
      661 aaacccaccc tgctacaatg accgactcaa tcttctcaag ggtgttgaat aatgatattt
      721 gttttactgc caataatagc ggcaactggc tcctttttat aacggaatag ctgaagcttc
      781 ccaatgtcat tacaattaga agaatctgat ttaccaagat aaaaatggtt gtctgtatac
      841 cgattttttt tatactctcc ttgttgttaa ttttatcccc caaaacaaac aaaaaagtat
      901 aataggatga aagtccggat attccaatac ctgcattgat caccggcaat atactcttat
      961 aggttacagg gcccaggaaa atcacctgta aatacggcat aagcataata aatataatca
     1021 gaaaagtgac agcaaacata ggaaacacaa tttcattcaa tcgtgcaaga gtaacaagtc
     1081 cataacgcaa taaatagacc accaaaacca aaatcgatat aataaatata ctcggattgg
     1141 tatcggggta aattgagcct accagtctaa ccgcaaaata catcacatac acacaaacca
     1201 aaagtgttgc ccacaaaaag tatattatac tcaatagctt gccgataaat ttcccggcaa
     1261 tatcgctgta tatatgcatt aacgaatcat tcttatactt cttataaatt ctgttccata
     1321 ccaatacatt caaaaacaat ataacagcag caacaatcgg tgcaagccag gctgcctctt
     1381 gtgctgtctg tgccgcaata acaggaacaa gcctgattgc aggggatgca ataacggtca
     1441 ggaacaaaaa catagcctgc ctgatagaaa ttttatcttt gtcgtataac attgagaacc
     1501 tccttttaaa actcttgccc tataagcctg agcatatgtt gtgcaaggct tgttctgtat
     1561 gggtttgaca aatataacaa acccaatgcc agactaatca ctgcgagccc taaatataca
     1621 aatatctcct tcttttgctg tttcttaacc atacttttta tttcaataaa gctcaggatg
     1681 actacaaata taaccaattt aatcacttac cgccttctcc ttccacagca ttaggttctc
     1741 tgatatcata ggttcttctg atctttgatt ttacttccac actgaatttt attttcggga
     1801 accaagtttt ccagtctttt tcatacaatt tcttccattt gatgggatac ttacggtaaa
     1861 aattcagagc tgtgctgaaa aaatcaagtc caatttcctt ctgggcatat tcaataactc
     1921 tttctatttc ctgcttaaca agccttgact gctcttgttc caaataaatg aaggtatcat
     1981 tgttaaaaat gtcttcgttg gaatctattt cgccaatatt gctttccatg tttaccttga
     2041 cctttaaaga caagttcccg tcctttattt caggataaaa tttagttttg gaatttatta
     2101 tttctaaaga gacctttgag cctttcctgg tgtttacaac ataaatgccg gattttacct
     2161 tatttctcaa caggtttaac cctcttccca tattttcatc cagataaccg gccagcttgt
     2221 cttcgtcgaa tatagcaaac ccagccattt taacatcgat tacatcctca tcctgcccct
     2281 ttgtcacatt aaccatctca acacatggga tataaaggga cagacatttc atatcctgta
     2341 tgtacataac ctcgataaga ttaacaggat ttgaaaccga ttgtcctccc aaactgttaa
     2401 gcagcccatt caaccggtca aatattaacc ggtcgcctct gtctgctttt tccatcaatt
     2461 cttccgcaca acaacccttc atgacaaaaa catttatatt cattctgacc tcatggtcac
     2521 gggcaaaaaa atccaggtag ttatgtatac ctttttttgc agcttcctct cctattaaaa
     2581 caaattcaag atgaccccaa aaaggtcttt tacccaaata ggtgctgaaa tttctgaccg
     2641 cttcaaacat agtccttcct tcagaggaaa tttttgaaga ctccttttcg cctacagaac
     2701 cgccactgcc tatttttatc ctttgcgaag ccagagtcag ccttaccccg tcaggtattg
     2761 atttagccgt atctattgct atagacctcg caaactcaag gtcattgatt tcgtgtctgc
     2821 tcataatggg gttttcacag gctgtgaggg ttagagtaac aataaacagt aataaaagcg
     2881 aaattattct tctcacaatt ctacctccta gcttctcctc tttttattag ttgttttcaa
     2941 ttgtataggt cttttataca ttgttgaaaa cggcattctt ataagtgtat cctgcagctg
     3001 cttgtcatct gttgcaacaa agggtgcaag atacggaact ccaaagctct ccatttttga
     3061 ccaatgaatc agcagtatta aaccacccat acttaatcca tatagcccgg caaagcttgt
     3121 taaaatgact aaaccaaacc tccaaattct taaagcattg gagaaatcct gatccggcat
     3181 ggtaaagctt gccactgccg ttatcgatat gattaccaca acggcaggtg acacaagcct
     3241 tgcttcaaca gcagcctgtc ccactaccag ggcaccgact atagtaaccg cctgacttat
     3301 atttctcggc aatctcaatc ctgcttccag caaagcctca aaagcaatga gcataaatat
     3361 tacctctata aaccccggaa agggaacccc ttcttttgaa gctgtaatgg ataaggcaag
     3421 ctccgttggt atcatttcat gatgaaagct tgttatggca atataaaacc cgggcaaaaa
     3481 aagggtaact accattaaaa catatctcat aaacctgaag atagaactgg aaataaaatt
     3541 ctgggaataa tccttagggc cttgtaaaaa ttggcttaat gtccccggtg caacaaaagt
     3601 agtgggcaaa ccgtcaatta tt
//
LOCUS       DAOOJC010000251         3622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_19882_length_3622_cov_3.461452, whole genome shotgun sequence.
ACCESSION   DAOOJC010000251 DAOOJC010000000
VERSION     DAOOJC010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19882_length_3622_cov_3.461452"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLA01_07295"
     CDS             <1..449
                     /locus_tag="PLA01_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01137.1"
                     /translation="YQWQKKVEEGWTDIKGATSEQYRFSNSGYGQAGEYRCRVNYVYN
                     DTENNAAYYITTYSDVFAIEYVMRQAQMISFAADDKSKTVSITLKSKHKNHFYAPTGT
                     VTFRIEGKGYGLSYTCTEWLISNQQKELDILDFIRTCNGCIMAVLL"
     gene            337..612
                     /locus_tag="PLA01_07300"
                     /pseudo
     CDS             337..612
                     /locus_tag="PLA01_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517779.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            675..1490
                     /locus_tag="PLA01_07305"
     CDS             675..1490
                     /locus_tag="PLA01_07305"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPD01138.1"
                     /translation="MKTLFVSDLDGTILRSDETISQFTIDTINELTKRGVLFSYATAR
                     SLVTAKKVTKGLNSHIPVIVYNGTFVMDNATEEILIANYFDDGIEEIFCDLFKNGIYP
                     IVYSYIDGVEKFSYIPEKCTEGMKFFLNTRKDDKRKNEVRTIEDLTAGKCFYITCIDE
                     PEKLEPFLKKFKDRYHVVYSKDVYSDFQWLEIMPKTASKANAVLQLKEKLCCHRIVSF
                     GDGENDIDMFKISDEAYAVDNAIDDLKTVATGVIGSNDDDGVAKWLIAKTERL"
     gene            complement(1672..3426)
                     /locus_tag="PLA01_07310"
     CDS             complement(1672..3426)
                     /locus_tag="PLA01_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HPD01139.1"
                     /translation="MDFTMIADTLWVVLAAVLVFFMNLGFAAVETGFARSKNTVNILS
                     KNFIVFAVSSLGFMLLGWGLMFGGDNPLIGTQNLFILGDSNLDFYKDTLTSSVPFWGK
                     FFFQLVFCGTAATIVSGAVAERVKYISFIVFSFVLTLAIYPIVGHWIWGGGWLANLGF
                     LDFAGDTVVHSVGGWAALTGAIILGPRYGKYDKNGKPKAIPGHSMALAVIGLFVLWLG
                     WFGFNPGSTMSFQNPADVVHILVTTNTAAVAAVLTSTATSWIYIGKPDLGMTINGCLA
                     GLVAITGSCAYVSVTSSLIIGAIAGVIVVFAVLFFDRVKVDDPVGATSVHLVCGVFGT
                     LCVGLFAQEGVTSLSTVNGLFFGGGFSLLGTETIGIISVGAFVAASSALVWLIIKKTI
                     GIRVTLEEEIEGLDIGEHGNSAYPDFAIVAPIMATGNGNGDKVAAIIPVSTPKPSYGK
                     VSPEAAIPVINKAHPGAKMTKVTVITNQDKFTHLQSSLDKIGITGLTVTNVLGYGMQK
                     GHAEYFRGLPVKTRLLPKVQVDIVVCKIPVETVVDTIKKSLYTGNMGDGKIFIYDVEN
                     VIKISTGEEGYDALQDEE"
BASE COUNT         1119 a          708 c          781 g         1014 t
ORIGIN      
        1 agtaccaatg gcagaaaaaa gttgaagaag gctggacgga catcaaaggt gcaacatcgg
       61 aacaatatcg gtttagcaat tccggttatg gccaggccgg tgagtatcgt tgcagggtca
      121 attatgttta caatgatact gaaaacaatg ctgcctatta cattaccacg tattcggatg
      181 tcttcgcaat tgaatatgta atgcgacagg cacagatgat ttcttttgct gctgatgata
      241 aatctaaaac ggtttctatt acgctgaaat caaaacacaa aaatcatttt tacgcaccga
      301 ccggtacggt gactttccgt attgaaggaa agggttatgg tctttcttac acatgcacag
      361 agtggcttat ctcaaatcag cagaaagagc tggatattct tgattttatc aggacttgca
      421 acgggtgcat catggctgtg ctattataga gcgatacaaa caggagatgt atcaaaggtt
      481 gtaccgattg ataaaatgag tgtcataatt acgattgttc ttgcatttat tttcttacac
      541 gagcaactga ctgcaaagtc actgattgga tgtgtattga ttacagcagg tactctcatt
      601 atggtggtat agaataggtg acggttctct gttttaaata taatttactt ccgagagtta
      661 aaggagatgt agcattgaag acactgtttg tatctgatct ggatggaacg attctaagaa
      721 gtgatgaaac tatatcgcag ttcactatcg acacaatcaa cgaattgaca aaaagaggtg
      781 tacttttttc ctatgccaca gcccgttctt tggtgacagc taagaaagtt acaaaagggt
      841 tgaattctca tattccggtc attgtttata acgggacatt tgtcatggat aatgcgacag
      901 aagaaatcct gattgcaaac tattttgatg atggaattga agaaatattt tgtgatttat
      961 tcaaaaatgg tatctaccca atcgtatatt cttatattga tggggttgag aagttttcat
     1021 atattcctga aaaatgcaca gagggaatga agttctttct taatacgaga aaagatgata
     1081 agagaaaaaa tgaagtcaga acaatagaag atttaacagc gggaaagtgt ttctatatca
     1141 cctgtattga cgagcctgaa aaattagagc catttttaaa aaagttcaag gacagatatc
     1201 atgttgtcta ctcaaaggat gtttattcgg attttcagtg gcttgagata atgcctaaaa
     1261 ctgcatccaa agccaatgct gttttgcagc tgaaggaaaa gttatgttgt cacagaatcg
     1321 tgtcctttgg agatggagag aatgacattg atatgtttaa gatttcggat gaagcctatg
     1381 ctgtggataa tgcaattgat gatttaaaaa ctgttgctac cggagtgata ggaagtaatg
     1441 atgacgacgg tgtagcaaaa tggctcattg caaaaacaga aaggttataa gctgttgaac
     1501 atgttggttt cgcttcggta ccaagacttt gtgcaaaacc tgttactgaa aataaaagga
     1561 taaattgaga atgatatatc aattgcataa gccaggaaaa gggcaaggaa catattaggt
     1621 tcaagcctgc tatgttccct gctcatccat attttaatat tcaattcatt ctcattcttc
     1681 atcttgcagt gcatcgtatc cttcttcgcc ggtgctgatt tttatgacat tttcaacatc
     1741 gtaaataaag atttttccgt cacccatatt gcctgtatac agcgatttct ttattgtgtc
     1801 gactactgtt tctacgggta tcttgcatac aactatatct acttgaacct tgggaagaag
     1861 tctggttttt acaggcaaac cgcggaaata ctctgcgtgg cctttctgca taccataacc
     1921 aaggacattt gttactgtca gacctgttat gccgatttta tcaagggagc tttgaaggtg
     1981 tgtaaactta tcctggtttg taataacggt gacttttgtc atctttgctc cggggtgagc
     2041 cttattgata accgggattg cagcctccgg agataccttg ccgtaagaag gcttcggtgt
     2101 tgaaactggg attattgctg ctaccttgtc tccgtttccg ttgcctgtag ccataatagg
     2161 agcaacgatg gcgaaatcag gataagcgga gtttccgtgt tcaccaatat cgagaccttc
     2221 gatttcttct tctagagtta cacggatacc gattgttttc ttaataatca accatacaag
     2281 agcagatgaa gcagctacga atgcaccaac ggatataatt ccgatagtct ctgttccgag
     2341 taatgagaag cctccgccga agaataatcc gttgactgta gaaagactgg ttacgccttc
     2401 ctgggcaaaa agtcctacac agagtgttcc gaaaacaccg catacaaggt gtacggaggt
     2461 agctcccaca ggatcatcaa cttttacccg gtcaaagaag agtacggcga acacaacgat
     2521 gacacctgct atagcaccaa taattaacga gcttgtaaca cttacgtaag cacagctacc
     2581 ggttatagcg accagtccgg caagacatcc gttgatggtc ataccgagat ccggttttcc
     2641 aatgtagatc catgacgttg cggtggaggt aagtacagct gcaacggcgg ctgtattggt
     2701 ggtaactaaa atatgcacga catccgccgg attttgaaag ctcatagtgg agccgggatt
     2761 gaagccgaac cacccaagcc acaatacgaa aagaccgata actgccagag ccatactatg
     2821 gcctgggatt gctttgggct ttccgttttt atcatatttc ccataacggg gaccaagaat
     2881 aatggctccg gtcagagctg cccagccacc gacagagtga acaacggtat cgcctgcaaa
     2941 gtccagaaaa ccgagatttg ccagccagcc gccaccccaa atccagtgac ctacgatggg
     3001 gtaaatggca agggtcagta cgaaagaaaa aactataaag gatatatact ttacccgttc
     3061 tgctactgca ccggatacaa tggttgctgc agtaccgcag aaaaccaatt ggaagaagaa
     3121 ttttccccag aacggtacgc ttgatgtcag agtatcttta taaaagtcga gattggaatc
     3181 gcctagaata aaaaggtttt gcgtaccgat aagcgggttg tctccgccaa acattaaacc
     3241 ccaacccaga agcatgaagc ccagcgagga aactgcgaaa acaataaagt tttttgaaag
     3301 gatattaact gtattttttg atctggcaaa accagtttcg accgctgcaa aaccgagatt
     3361 cataaagaac accagtacgg cggcaagcac tacccataga gtatcagcta tcattgtgaa
     3421 atccataaat tattcctcct tttaaaaata aaggcgtgtg ttttattgaa gaatcaataa
     3481 aacacacgcc tttgtgtgca ttaagtttga tacatactct tttcaaaatt tcatcaagtt
     3541 attcacttgc aaggcgaagt tacgcgtaat cgatcacaca accaatagag accggaatat
     3601 attccagatt aatcctcacc ct
//
LOCUS       DAOOJC010000252         3588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_20137_length_3588_cov_3.625531, whole genome shotgun sequence.
ACCESSION   DAOOJC010000252 DAOOJC010000000
VERSION     DAOOJC010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20137_length_3588_cov_3.625531"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /gene="pglZ"
                     /locus_tag="PLA01_07315"
     CDS             <1..1434
                     /gene="pglZ"
                     /locus_tag="PLA01_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009534423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HPD01140.1"
                     /translation="DDMEGIIDKYCSKDYLIDRFYREFYSYYDRIEDTVPYEKLRELV
                     ENIYTNEYLAKVLPKWNAAITADESMTVMPLQRNFFDKYISNSKDRVVVIISDALRYE
                     TGYSLYEKLQDDAKCTVKMDAMMSTLPSYTRLGMAALLPHKQLTVTDDFKVLVDGLPC
                     DDLKQRESILQSYIPSSCCVQYDNIKAMRQAELREIFTGKEVVYVYHNQIDGRGEKPN
                     TENEVFSACLEAIDEIYSLIKRLSGRANTYHFIVTADHGFIYKRDKLKESDKIGNATG
                     KGLFVNRRFIISDTAIKEDGVACTSLGLILGNDDSKVVAYPVSSNVFKVPGGGQNYVH
                     GGSSPQEMIIPVIDVKTEKGHVETRPAQIMLVSMVQKITNLITTLDFIQSEPVSDVVR
                     ATSYRLFFISEDNEKISNECIYIADKKDVEPQKRIFRLRFNFKNMQYDKSKKYYLVAY
                     DEKNDLEILRHGVAMDIAFANDFGFRV"
     gene            1447..3525
                     /gene="brxL"
                     /locus_tag="PLA01_07320"
     CDS             1447..3525
                     /gene="brxL"
                     /locus_tag="PLA01_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HPD01141.1"
                     /translation="MSELQIESTGISSENKSEELLEKLHTYFAGKIVRKDLTKKIKEG
                     ANVPVYVLEYLLGMYCSSRDEDEINAGIENVKQILAENYVRPDESQKIISKLREYGSY
                     TVIDRITAKLNYKSDIYESEFSNLGLKGVPISEKYVTDYERLLCGGIWCIVQMEYFYD
                     EADKNRCPFIITKLTPIQMPSLDFEEVKAARANFTREEWIDIILRSTGMEPFQFNDRV
                     KWLHLARLIPLVENNYNLCELGPRGTGKSHIYKEISPNSILVSGGQTTVANLFYNMAN
                     KTVGLVGLWDCVAFDEVAGITFKDKDGVQIMKDYMASGSFARGKEEKNAYASMVFVGN
                     INQSVDVILKTSHLFEPFPDAMANDTAFFDRMHCYIPGWEIPKYRPEYFTNDYGFITD
                     YFSEIMRELRKISYADAIDKYFKLGNNLNQRDVIAVKKTVSGLVKLIHPDGQYNKESV
                     EEILRFALELRRRVKEQLKKIGGMEFYDVNFSYIDNETFNEEFVSVPEQGGGKLIPEG
                     MGKPGHVYTVSHGKNGMIGVYKLEMQMTTGTGKFERTGIGSDREAKEAVDTAYCYLKA
                     NARNISGTISTTTKDYLMHVQDLNGIGMTKYLTLPALVAICSVALNKPVISSAVILGD
                     LSIGGTIIKVEELANTLQVCLGSGAKKVLLPITSAVDLGTVPSELIGCFNIIFYQSAE
                     DAVFKALGVE"
BASE COUNT         1115 a          554 c          874 g         1045 t
ORIGIN      
        1 gatgatatgg aaggtataat cgataagtat tgcagcaagg attaccttat agatagattt
       61 tatcgtgaat tctattcata ttatgacagg attgaagata ctgtgcctta cgaaaaactg
      121 agggaacttg ttgagaacat ttataccaat gaatatttgg caaaagtttt accgaaatgg
      181 aatgcggcca ttacggcaga tgaatctatg actgtgatgc cgttgcagcg taattttttt
      241 gacaaatata ttagtaacag caaggacagg gttgtggtca tcatatcgga tgcactccgt
      301 tatgagactg gatactcact ctatgagaag ttgcaggacg atgctaaatg tactgtgaaa
      361 atggatgcaa tgatgagtac attgccttct tacacccgcc ttggaatggc ggcattattg
      421 ccacacaagc agcttacagt aactgatgat tttaaggttt tggttgatgg attaccttgc
      481 gatgatctca aacaacgtga gagcatttta caaagctata taccaagcag ctgctgcgta
      541 cagtatgata atataaaggc aatgagacaa gcagagcttc gtgagatttt tacaggtaag
      601 gaagtggtct atgtctatca caaccaaatc gatggccgag gagagaagcc caatacagag
      661 aatgaagtgt ttagtgcttg cttggaggct attgatgaga tatacagttt gattaaacgt
      721 ctttcgggac gtgcaaatac ataccacttt attgtgacag cagatcatgg ttttatttat
      781 aagagggata agcttaaaga gagtgataag attggaaatg caaccggtaa ggggttgttt
      841 gtaaatagac gattcataat atctgacacg gcaatcaaag aggatggtgt ggcttgtacg
      901 tctcttgggt tgattttagg taatgacgac agcaaggtag tggcttatcc ggtaagttca
      961 aatgtgttca aagtaccggg aggaggtcag aattatgtac atgggggatc ttcaccgcag
     1021 gaaatgatta tcccggtcat agatgtgaag actgaaaagg gacatgttga aaccagacca
     1081 gctcagatca tgttggtaag tatggttcag aaaataacaa atcttattac aacactggat
     1141 tttatccagt cggaaccggt cagcgatgtt gtgagagcta ccagttacag gttgtttttc
     1201 atttcagagg ataacgaaaa gatttccaat gagtgcattt atattgcgga taagaaagat
     1261 gttgaaccac aaaagagaat tttccgtttg cgtttcaact ttaagaatat gcagtatgat
     1321 aaatctaaga aatactatct tgtagcttat gatgaaaaga atgatttgga aatcctgcgt
     1381 cacggtgtag caatggatat tgcctttgct aatgactttg gatttagagt ataggtggtg
     1441 aaaaatatga gcgaattaca aatagaatcg acaggaattt cgtctgaaaa taaatcagag
     1501 gagttgttgg aaaaactgca tacctatttt gccgggaaaa ttgtacgtaa agaccttaca
     1561 aagaaaatca aggaaggtgc aaacgttcct gtctatgttc tggaatatct tcttggaatg
     1621 tactgttcat cgagggatga ggatgaaatc aatgccggaa tcgagaatgt aaagcagatt
     1681 cttgccgaga attatgtgcg gcccgatgag tcacagaaga tcatttcaaa gctgcgtgag
     1741 tatggcagtt atacagtaat cgacaggatc actgcaaagt tgaattacaa aagtgatata
     1801 tatgaatctg agttttccaa cctggggtta aaaggggtac ctatttcaga gaagtacgtc
     1861 actgattatg agcgtttact atgcggtggt atttggtgta ttgtacagat ggaatatttt
     1921 tatgatgagg cagacaaaaa tcgttgccca tttataatca ccaaactgac accaattcag
     1981 atgcctagtc ttgattttga ggaggttaaa gcagctcgag cgaactttac aagggaagag
     2041 tggattgaca ttatactgcg aagtacaggt atggagcctt ttcagtttaa tgatcgtgtt
     2101 aaatggttgc atcttgcaag gttgatccca cttgttgaga ataattataa tctatgtgaa
     2161 ttaggtccga gaggaaccgg caaatcccat atttacaagg aaattagtcc taatagtatt
     2221 cttgtatcag gaggacaaac gaccgttgcc aacctgtttt ataacatggc aaataaaacc
     2281 gttgggctgg ttggactctg ggattgtgtt gcttttgatg aagttgcggg aatcacattt
     2341 aaggacaagg atggcgtcca gataatgaaa gactatatgg catcgggttc ttttgcaaga
     2401 ggtaaggaag aaaagaatgc ttatgcctcg atggtctttg tggggaatat taatcagagc
     2461 gtggatgtta tattgaaaac atcacacttg tttgaaccat ttccggatgc gatggcaaat
     2521 gacactgctt tttttgaccg tatgcattgc tatataccgg gatgggagat acccaaatat
     2581 cgaccggagt attttaccaa cgattatgga tttatcaccg attatttttc ggaaataatg
     2641 cgggaactta gaaaaatttc ttatgctgat gctattgata aatatttcaa gcttggaaat
     2701 aacctgaacc agcgtgatgt gatagcagtg aagaagacgg tttccggcct tgtgaagctt
     2761 attcatcccg atggacaata taacaaagaa agtgttgaag aaatcctgcg ttttgctttg
     2821 gaattgcgac gaagagttaa agagcaattg aagaagattg gaggtatgga gttctatgat
     2881 gtgaatttct catatataga caacgaaact tttaatgagg aatttgtttc tgtgcctgag
     2941 cagggtggcg gcaagcttat accggaggga atgggaaaac cggggcacgt ctatacggtt
     3001 tcccatggaa agaacggtat gatcggcgtt tataaactgg agatgcaaat gacgacaggt
     3061 actggaaaat ttgaacgtac cggtattggt tctgataggg aagcgaagga ggcagttgac
     3121 acggcttatt gctaccttaa ggccaatgct cgaaatatca gtggcaccat aagtacaaca
     3181 acaaaggatt atctgatgca tgttcaggac ctgaatggca ttggtatgac caaatacctg
     3241 accctacctg cattagttgc aatttgctct gttgcactga acaaacctgt tattagcagt
     3301 gctgtaatac tgggtgattt gagtattggc ggtactatta tcaaggtgga ggaattagcc
     3361 aatactctgc aggtgtgttt gggcagtggt gcaaagaagg ttttgcttcc gattacttca
     3421 gcagttgacc ttgggactgt tccgtctgag ttaatagggt gctttaatat aattttttac
     3481 cagagtgctg aggatgcagt gtttaaggca ctaggtgtgg agtgattttc acatttggca
     3541 tgatattaat aaactatata ttaaaaaggt acttaatatt attaatga
//
LOCUS       DAOOJC010000253         3541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_20506_length_3541_cov_3.658635, whole genome shotgun sequence.
ACCESSION   DAOOJC010000253 DAOOJC010000000
VERSION     DAOOJC010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20506_length_3541_cov_3.658635"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1425)
                     /gene="trmFO"
                     /locus_tag="PLA01_07325"
     CDS             complement(118..1425)
                     /gene="trmFO"
                     /locus_tag="PLA01_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HPD01142.1"
                     /translation="MIDYINVIGAGLAGCEAAWQIASRGIKVKLYEMKPKAYSPAHHM
                     DTFAELVCSNSLRSAQIENAVGLLKEEMRTLGSIIMECADATKVPAGGALAVDRTLFS
                     KTVTDRIMNNKNIEVVNEEVTCIPKEGITVIATGPLTSDALSKHLKDFIGEQYLHFFD
                     AAAPIVTFESINMGRAFKAARYNRGTEDYINCPMSKEEYEIFWNELVNAEMAEVKDFD
                     REIVFEGCMPVETMAKRGIDTLRFGPLKPVGLTDPNTGKEAYAVVQLRQDNSEGTLYN
                     MVGFQTRLKWPEQKRVFSLIPGLENAEFVRYGVMHRNTYINSPLLLDAAYRLKKSPDI
                     YFAGQITGVEGYVESASSGLVAGINASMSLLGKKDVIFPKSTAIGALSQYISNSSVKD
                     FQPMNINFGLMERKEIKIRDKRKKNYELAVKALEDVKRIMSEY"
     gene            complement(1418..3520)
                     /gene="topA"
                     /locus_tag="PLA01_07330"
     CDS             complement(1418..3520)
                     /gene="topA"
                     /locus_tag="PLA01_07330"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPD01143.1"
                     /translation="MAEKLVIVESPAKANTIGKFLGKGYKIVASVGHVRDLPKSQMGV
                     DIENDFAPKYITIRGKGDIISKLKKEAKNAKTVYLATDPDREGEAISWHLANLLNIDE
                     KEKCRITFNEITKNAVRNAIKVPREIDMDLVDAQQARRILDRIVGYQISPILWKKVKK
                     GLSAGRVQSVATRLICDREDEIEKFVPDEYWTITAKLLKKNLKMPFNAKFYGLGNKKI
                     ELKNEDEVNNILIEIKNSAFKVSKVKKGEKRKTPNAPFTTSTMQQEASRKLGFSTKKT
                     MIVAQQLYEGVDIKGIGAVGLVTYIRTDSTRVSAEAQQNTAEYIKLKFGEEYLPKEKP
                     VYKGKSSSQDAHECIRPTSVDMDPESVKDSLSKEQYKLYKLVWERFVASQMTAAVYDT
                     ISADIEAGKYIFKASGSTIKFAGYTHLYQEGKDEESEEADGDIPELVENEELMQKGIE
                     PKQHFTQPPPRYTEASLVKTLEEKGIGRPSTYAPTITTILARGYIVKDGKTLIPTELG
                     RIVTDIMKNYFQEIVDVEFTAQLEKKLDEVEEGGKRWVDVMKNFYSQFVDVLKEAEAQ
                     IGNIEIPDEVTDEICEKCGRNMVIKMGRNGRFLACPGFPECRNAKPILVDAGVTCPKC
                     GGKVYIKKTKKGRKYLGCENNQSEPKCDFMTWEMPAKENCPQCGSFLLKKYSGKKAQF
                     KCSNENCDFKKTGEEKDD"
BASE COUNT          888 a          899 c          557 g         1197 t
ORIGIN      
        1 acaagtattt tttataaaat ttacaaatac tttaaaaaaa tcaatatatt attcttaaca
       61 aatatgaagg aaggtgctct aaaaaaatca atcttccggg gcactttcct tcaaatatca
      121 atactcactc attatcctct ttacgtcttc caaagcctta actgccagtt cataattctt
      181 tttccttttg tcccggattt tgatctcttt cctttccata agtccgaaat taatgttcat
      241 aggttgaaaa tccttcacgc tgctgttgga tatatattgg ctcaaagccc caatggcagt
      301 gcttttcggg aatataacat cctttttgcc cagcaaactc atagaagcat ttattcctgc
      361 cactaaaccc gaagatgccg attcaacata cccctccact ccggttattt gaccggcgaa
      421 atatatatcg ggagactttt tgagcctgta agcagcatcc aaaagcaacg gtgagtttat
      481 atatgtgttt ctgtgcataa ccccatacct gacaaactca gcattttcca gacccggtat
      541 taaactgaac accctttttt gttcaggcca cttaagtcta gtctgaaaac ccaccatgtt
      601 atacagcgta ccctcactgt tatcctgcct caattggaca accgcataag cctccttccc
      661 cgtgttggga tcggtaagac ctactggctt tagcggaccg aatcttaacg tgtcgatacc
      721 cctttttgcc atggtttcaa caggcatgca tccctcgaag acaatttccc tgtcaaaatc
      781 ctttacctcc gccatttcag cattgaccaa ctcattccag aaaatctcat attcttcttt
      841 gctcatcggg caattgatgt agtcttcggt ccctctgtta tagcgtgctg ccttaaaagc
      901 ccggcccata tttattgact caaaggttac aataggagct gccgcatcaa agaaatgaag
      961 gtattgctct cctataaaat ctttgagatg ctttgacagg gcatcagagg ttaagggacc
     1021 ggttgcaata acagtaattc cctctttcgg tatgcatgtt acctcttcat ttaccacttc
     1081 tatattttta ttattcataa ttctgtcagt caccgttttt gagaacagcg ttctgtctac
     1141 tgcaagtgcc cctcccgcag gcactttcgt tgcatccgca cattccatta ttattgagcc
     1201 caaagtcctc atctcttctt tcaaaagtcc cacagcattt tcaatctgtg ccgaacgaag
     1261 agaattactg cacacaagct ctgcaaaggt atccatatga tgtgccggcg agtatgcttt
     1321 tggcttcatc tcataaagct ttacctttat tcctcttgag gctatttgcc atgctgcttc
     1381 gcatcctgcc aatccggcac cgataacgtt tatataatca atcatctttt tcctctccgg
     1441 ttttcttaaa gtcacagttt tcattactgc atttaaattg tgccttcttt ccggaatatt
     1501 tcttaagcaa aaagcttccg cattgaggac aattctcttt cgcaggcatt tcccatgtca
     1561 taaaatcgca tttgggttca ctttgattgt tctcacatcc aagatacttt ctacccttct
     1621 ttgttttctt tatatatact ttaccaccac atttgggaca tgtaacaccc gcatccacaa
     1681 gaatcggctt tgcatttctg cattctggaa atcccggaca ggccaaaaac ctgccatttc
     1741 tacccatctt tattaccatg ttgcggccgc acttctcaca aatctcatcg gttacttcat
     1801 cggggatttc aatattccct atttgagctt ctgcctcctt aagcacatcc acaaactggg
     1861 aatagaaatt tttcatgacg tctacccacc tcttgccgcc ttcttcgact tcatcaagct
     1921 ttttttccaa ttgtgcggta aactcaacat caacaatttc ctgaaaataa ttcttcatta
     1981 tatccgttac tattcttccc aattcagtag gaatcaatgt cttcccgtct ttcactatat
     2041 aaccccgggc aagaatagtg gtaatagtgg gtgcgtaagt actcggcctg cctatgcctt
     2101 tctcctcaag agttttgaca aggctggcct ctgtatacct tggaggtggc tgggtaaaat
     2161 gctgtttggg ttcaatccct ttttgcatta actcctcatt ttccacaagc tccggtatat
     2221 ctccgtctgc ttcctcgctc tcctcatcct ttccttcctg atagaggtga gtatatcctg
     2281 caaactttat agtagacccg cttgccttaa aaatgtattt acccgcttca atatcagcac
     2341 ttatagtatc gtaaacagct gctgtcatct ggcttgctac aaatctctcc cataccagct
     2401 tgtacaactt atactgttct ttcgacagtg aatcctttac cgattcaggg tccatgtcaa
     2461 ccgatgtggg tcttatacac tcatgagcat cctgagaaga agacttgcct ttataaacgg
     2521 gtttttcttt cggcaaatac tcctcaccaa attttagctt aatatattct gcagtattct
     2581 gttgtgcctc ggcagaaacc cttgttgagt cagtacgtat ataagtaact agaccaaccg
     2641 caccgatacc ctttatatct acaccttcgt aaagctgctg tgcaacaatc atggtctttt
     2701 ttgtactaaa ccccagcttt cttgaagctt cctgctgcat tgtgctggtg gtaaagggtg
     2761 cattgggggt tttcctcttc tcaccctttt ttactttact tactttaaag gcactgttct
     2821 ttatttcaat aagtatatta ttaacttcat cctcattttt taattcaatc tttttgttac
     2881 caagcccgta aaacttagca ttaaagggca tttttaaatt tttctttagg agctttgccg
     2941 taatggtcca gtactcatcc ggtacaaatt tttctatttc atcctcccta tcgcagataa
     3001 gccttgttgc caccgactga accctgccgg cactcaatcc tttctttacc tttttccaga
     3061 gaatcgggct tatctgatat cccacaattc tgtccagaat tcttcttgcc tgctgtgcat
     3121 caaccagatc catatcaatc tctctcggca cctttatggc attcctgaca gcatttttag
     3181 taatctcatt aaatgttatt ctgcacttct ccttttcatc tatatttaat agatttgcaa
     3241 gatgccatga gatagcctca ccctcacggt cagggtcagt ggcaagataa accgtctttg
     3301 catttttagc ttcctttttg agctttgata ttatatctcc ttttcctcta atagtaatgt
     3361 acttcggagc aaaatcattt tctatatcaa ctcccatctg actcttggga agatccctca
     3421 cgtgaccgac agaagcaact atcttgtagc cctttcctaa gaatttcccg atagtgtttg
     3481 ctttagcggg agactcaaca ataaccaatt tttcagccat ttttatcctc cattttaatt
     3541 c
//
LOCUS       DAOOJC010000254         3525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_20620_length_3525_cov_3.959366, whole genome shotgun sequence.
ACCESSION   DAOOJC010000254 DAOOJC010000000
VERSION     DAOOJC010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20620_length_3525_cov_3.959366"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2414
                     /gene="essC"
                     /locus_tag="PLA01_07335"
     CDS             <1..2414
                     /gene="essC"
                     /locus_tag="PLA01_07335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HPD01144.1"
                     /translation="SMATLFHPYEVGFVIIDFKGGGMANQFKDLPHLMGSITNIEGRE
                     IDRSLMSIKAELIKRQAIFKEYNVNHIDSYIKLYKENKAKQPLPHLIIIVDEFAELKS
                     ECPDFMKELISAARIGRSLGVHLILATQKPSGVVDNQIWSNSKFKLCLKVQTKEDSNE
                     VIKTPLAAEIVEPGRAYLQVGNNEIFELFQSAYSGAKADNSSVVTGNVFELYKLNLWG
                     KKQLIYSNRKKSSDKNTDTQLNVLVNYIKNYCMANSISPLPGICLPSLPDIINFNDVK
                     QPPKDEIDTKVAIGMYDDPSNQIQDTVVLNISESHTLIIGSSQYGKTNLIQTIIRGIM
                     SNYTPDEVNIYMLDFGSKILKNFSDLNHVGGVVTLSEDEKLKNFIRLIKTEIAFRKDK
                     LLDAGISSFASYREAGYRDMPHIVIVIDNFIGFKENYTEYDDDMLGICREGIAVGISI
                     ILSNLQTSGIGYKYLSNFANRIALYCNDSGEYGNIFDRCRMQPKDVPGRALISIDRRI
                     LECQCTLGFDGEKEVDRVENMKKIIDITNDKEENRGKKARVIPEIPRLLTEEYIRNNY
                     AKTQLKKYQIPIGLDYDEVDLVTLDLLKLSVIAVTGRENSGRTNFLKIIMNNIQKNIF
                     NYPVRAYIIDDVERHLKKMDDYGFVDKYTVDANEFEVILDEVYSELEDRQEIVIDEGT
                     EGIEKEPLLLIIVQNRDAIGALSSNKKAVEVYKKITRQYKALKVCFVFSDIENAPVAY
                     GSPEILKNIKENRKAVIFDDLNNHKVFDIPTNALRTFKKPIEKGDAYFIMGNELIKIK
                     TIFQE"
     gene            2430..2744
                     /locus_tag="PLA01_07340"
     CDS             2430..2744
                     /locus_tag="PLA01_07340"
                     /inference="COORDINATES: protein motif:HMM:NF017793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HPD01145.1"
                     /translation="MADSQGGVKHLYTKDMKETSLELQRNIDDFQDAVEKVSKATTKL
                     LSTWAGKSRDRFETQYNLLFGQLKDFEGALYDIYDALVDSETAYIDADEALKKEFNIA
                     NK"
     gene            2837..3268
                     /locus_tag="PLA01_07345"
     CDS             2837..3268
                     /locus_tag="PLA01_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01146.1"
                     /translation="MSISSLKEQRREKKAELKKLEKRKKELEKILRSLEWEFHDEATA
                     FNYNLTNSTTNLEYGILNNETAYKNVEDMNGDKEDSPDTDTQLSDARSSLSAEINSLS
                     RDIGYLKEDIRYLDREIERKQAEQLKDGINAIKNAVEDIFN"
BASE COUNT         1248 a          527 c          799 g          951 t
ORIGIN      
        1 tatcaatggc tacgttattc catccttatg aggttggatt tgttattata gactttaaag
       61 ggggaggaat ggccaatcaa tttaaagacc ttccccactt gatgggttca ataaccaata
      121 ttgagggaag agagattgac cgatctctca tgtcgattaa ggctgaactc ataaaaaggc
      181 aggcgatttt taaagaatat aatgtaaatc atatagactc ttatataaaa ctttataaag
      241 aaaataaggc gaagcaacct ctgccccatt tgattattat tgtggacgag tttgctgaac
      301 ttaaaagcga gtgccccgac tttatgaagg agcttatcag tgctgccaga ataggaagaa
      361 gcctcggagt gcatttaata cttgctaccc agaaaccctc gggggttgtg gacaatcaga
      421 tatggagtaa ctcaaagttc aagctttgtt taaaagtgca gacaaaggaa gacagtaacg
      481 aggttataaa gactccgctt gcagcggaaa tagtggagcc gggccgggcg tatttgcagg
      541 tgggaaacaa tgaaatattt gaactttttc agtctgcata cagcggtgca aaagcagata
      601 attcaagtgt agtaaccgga aatgtgtttg agctgtacaa gcttaatttg tggggaaaaa
      661 agcagcttat atattcaaac aggaaaaaaa gcagcgataa gaataccgat acccaactga
      721 atgttttggt aaattacata aaaaattact gtatggcgaa ttcaatcagt cccctgccgg
      781 ggatatgtct tccatctctg cctgacatta ttaattttaa tgatgtcaaa cagccgccaa
      841 aggatgagat tgatacaaaa gtcgctatcg gaatgtatga cgatccttcc aatcagattc
      901 aggatactgt tgtcctgaat atttcagaaa gtcatacgct gattatcggc tcatcccaat
      961 atggaaaaac caacttgatt caaaccatta tcaggggtat aatgagtaat tatactcctg
     1021 atgaagtgaa catatatatg ctggattttg gttcaaaaat ccttaaaaat tttagcgatt
     1081 taaatcatgt ggggggagtt gtgacactat ctgaagatga aaaacttaag aactttatcc
     1141 gattaataaa gacagaaatc gcttttagaa aggataaact tttggatgca ggtatcagtt
     1201 cctttgcatc ctatcgtgag gcaggatata gagacatgcc ccacattgtg atagtaattg
     1261 acaatttcat aggttttaaa gaaaactata ccgaatatga tgatgatatg ctcggtatat
     1321 gcagggaagg gatagcagtg ggtataagta taatactgtc aaaccttcaa accagcggaa
     1381 tcggctataa atacctttct aattttgcta accgcatagc attatactgc aatgactccg
     1441 gtgaatatgg caatatcttt gaccgatgcc gcatgcagcc gaaagatgtc ccgggacggg
     1501 cacttatttc aatagatcgg cgtattcttg aatgccagtg cactctaggc tttgacggag
     1561 aaaaagaagt agatagagtt gaaaacatga aaaaaatcat agatattacc aacgacaaag
     1621 aggaaaaccg gggtaagaag gcacgggtta ttcctgaaat accgagatta ctcacagagg
     1681 aatacattag aaacaattat gcaaaaacgc agcttaagaa gtatcaaatt cccattggtt
     1741 tggactatga tgaggttgat ttggtaacgc ttgatttgct gaagctatcg gttattgctg
     1801 ttacaggcag agagaacagc ggaaggacaa acttccttaa aataattatg aataacatcc
     1861 aaaaaaatat ttttaactat ccggtcaggg catacataat cgatgatgtt gaacgacatt
     1921 taaagaaaat ggacgactat ggctttgttg acaagtacac tgttgatgcg aatgagtttg
     1981 aggtaatttt ggatgaagta tacagcgaac ttgaggacag acaggagatt gttatcgatg
     2041 agggaacaga agggattgaa aaggaaccgc ttttattgat aattgtacaa aacagggatg
     2101 caataggtgc attgagctca aacaaaaagg cagtggaggt ttataagaag attacaagac
     2161 agtataaggc actaaaagta tgttttgttt tttcggatat cgaaaatgct ccggttgcct
     2221 atggttctcc cgaaattctt aaaaacatca aggagaacag gaaagcggtt atatttgatg
     2281 acctcaataa tcataaagta tttgatattc ctacaaatgc tttaaggaca tttaaaaagc
     2341 ccattgaaaa aggtgatgcc tactttataa tgggcaatga acttattaaa ataaaaacca
     2401 tatttcaaga atagtgggag gggatatata tggcggatag tcaaggcggg gttaaacatc
     2461 tatatacaaa agacatgaag gaaacatccc ttgagttgca acggaatata gatgattttc
     2521 aagatgccgt agaaaaggtg agtaaggcaa caacaaaact gttgtcaaca tgggcgggta
     2581 aatcgagaga tagatttgag actcaatata atctcttgtt tgggcaactt aaggattttg
     2641 agggggcact ttatgatatc tatgatgctc ttgtagatag tgaaactgca tatatagatg
     2701 cggacgaggc acttaaaaaa gagtttaata ttgctaacaa atagaatcta tcgtttttcc
     2761 ggagcggcag aatttaaaac agaaagctgt tatctaagaa ctgccggaag atgataggag
     2821 aggagttggt gggaatatgt caattagcag cttaaaagaa cagcgaaggg aaaagaaggc
     2881 agagctcaaa aaacttgaaa agagaaaaaa agaacttgaa aagattttac gaagtcttga
     2941 gtgggagttt catgacgaag caactgcatt taattataat cttactaaca gtactacaaa
     3001 cctggagtat ggcattttaa ataatgagac tgcatataaa aatgtggagg atatgaatgg
     3061 ggataaggag gacagccctg ataccgacac tcaactatcg gatgcaagga gcagtctttc
     3121 tgcagagata aacagtttaa gcagagatat tgggtattta aaggaagata tacgctatct
     3181 tgaccgggaa attgagagga agcaggccga acaattaaaa gatggaatta atgcaataaa
     3241 gaatgcagta gaggatattt ttaattaaat ctgacaaagg tttgagtatt tattgtcagg
     3301 caaggtaatg gttgattaaa aaggagcaga cattttgcaa ggaggtgtta aaatgggtgt
     3361 attaaaaata aattattctg aacttattac ttctgttaaa agtgccgaaa aggcttctaa
     3421 aaaagcgaaa tcctatgcgg aagagcttga aaaaaaggtg tgtaaaaaga tttccggtct
     3481 tgagggtgga ggttcggata atgtaaataa tgctgattat tttgt
//
LOCUS       DAOOJC010000255         3521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_20651_length_3521_cov_4.318234, whole genome shotgun sequence.
ACCESSION   DAOOJC010000255 DAOOJC010000000
VERSION     DAOOJC010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20651_length_3521_cov_4.318234"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PLA01_07350"
     CDS             <1..424
                     /locus_tag="PLA01_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPD01147.1"
                     /translation="ISRIIHLKTYINDSFMDLYPGDGLIISTPTGSTAYSLSAGGPLV
                     EPDVDLIIATPICPHLLYSRSFITTGDRVVKVVVDESYEYEAMVTVDGQKGYEVKGGD
                     LIVTKKSQQRFPMIRLNNKRNFFDVLRGKIYDRGESMG"
     gene            426..905
                     /locus_tag="PLA01_07355"
     CDS             426..905
                     /locus_tag="PLA01_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HPD01148.1"
                     /translation="MKNKRQAKILEIIENEIIETQEEIAQRLKDAGMDVTQATISRDI
                     KELRLIKVMTADGRYKYAPFSKTENVLLNRLMTIFSESYVSCDYANNIIVVKTLPGMA
                     QASASAIDSLKRSEIVGSIAGDDTVFIVCRNEKIAHDFVDRLKKLAKPSLEGSNIFE"
     gene            939..2666
                     /gene="recN"
                     /locus_tag="PLA01_07360"
     CDS             939..2666
                     /gene="recN"
                     /locus_tag="PLA01_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPD01149.1"
                     /translation="MLQRLEIENIAIIDRVEIEFGEGLNVLTGETGAGKSIIIDSINA
                     ILGERMSKELIRTGRDKAVVEAVFHVNPDRLSDLFESFGIECEEDGALIISREFTASG
                     KNICRINGRIAAVSMLKKIGERLIDVHGQHDNQSLLRTESHIELLDSFADDKINILKD
                     EYLKHFESYREIKNRLKSLTGDAGERERKIDILKFQIDEIKKAKLKAGEEEEIEKQRE
                     VLLNYEKIISTLSNAYEALDSGEKLGISALDRINKSVSDFCNIEDYDEKFSTLKKRLE
                     AVSYELEDIVSEIRDLRDGIEYEPSVLEQLEERLDVLYRLKKKYGDSIEGILEYLENA
                     KKELDELVNNEEIINRLKEDLKREDEKLYKLAKDINSERVKTAGLLENRIGSEFCDLE
                     MKNTRLKVNIEFDDSMNDGERRYNNNGLDRVEFLISTNAGEPLKPLAKIASGGEMSRI
                     MLAIKTILANVDKMPTMIFDEIDIGISGIAAQKVGEKLCYIAKNHQVISVTHLAQIAC
                     MADNNYYIEKVTKDGSTQTVVYCLDEEGKRNEIARIIGGAKVSDIALKHAEEMLDYAR
                     EFKKANGKG"
     gene            3031..>3521
                     /locus_tag="PLA01_07365"
     CDS             3031..>3521
                     /locus_tag="PLA01_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HPD01150.1"
                     /translation="MKYIKPTKRKVILFLVICFAVMTITYLRMLFSLPNQITLFENKE
                     YNYNFKSPFLVNLEAENDEILIFESKDSKVSNKKNGFNDSIMIKAKKLGRTSLSLRLF
                     GLIPIKTMQVDVVPYKEVIACGNTVGVKIKVDGILVIGLSDVETVNGERLLPARDSGL
                     KPG"
BASE COUNT         1203 a          535 c          823 g          960 t
ORIGIN      
        1 tatatcgaga ataatacacc ttaagactta tattaacgat tcttttatgg atctctatcc
       61 gggggacgga cttattatat caactcccac tggttctaca gcatattccc tttctgcagg
      121 aggacctttg gtagagcccg atgtggatct tatcatagca acgcctatat gtccgcacct
      181 tttgtattcg agatccttta taactacggg agacagagtt gtaaaggtgg tagttgacga
      241 gagctatgaa tatgaagcaa tggtaaccgt tgacgggcag aagggctatg aggttaaagg
      301 cggagatttg attgtgacaa aaaaatctca gcaaagattt cctatgataa ggctaaacaa
      361 taagaggaat tttttcgatg tacttagagg caaaatctat gacagaggag agagcatggg
      421 ataatatgaa gaacaagaga caggctaaaa tacttgagat tatagaaaat gagatcattg
      481 agactcagga ggaaattgca caaaggttaa aggacgcagg aatggatgtg actcaggcta
      541 caatttcaag ggatatcaag gagttaaggc ttataaaagt aatgacggct gatggccgct
      601 ataagtatgc tcccttttct aaaaccgaaa acgttttgtt aaacaggctt atgacgattt
      661 tttcagagtc ttatgtttca tgtgactatg caaataatat tattgtggtt aaaactttgc
      721 cgggcatggc tcaggcttcc gcatccgcaa tagattcttt aaagcggtct gagattgtgg
      781 gatcaattgc aggagacgat acggtgttta ttgtctgcag gaatgaaaag attgcccatg
      841 attttgtcga taggttgaaa aagcttgcaa agccgagtct tgaaggatca aatatatttg
      901 agtaaaacag aaattcaaaa tgaattgagg ttttagacgt gcttcaacga ttggagatcg
      961 aaaacatagc tataattgac agggttgaga ttgaattcgg agaagggctg aatgtgctaa
     1021 ccggagaaac cggggcaggt aagtctatta ttatcgattc aatcaatgca atcctgggag
     1081 aaagaatgtc aaaggagctt atccggactg gacgggataa agctgtggtt gaagctgttt
     1141 ttcatgtaaa tcccgacaga ctgtccgatc tttttgaaag ctttggaatt gaatgtgaag
     1201 aagacggtgc tctgattatt tcaagggagt ttaccgcttc aggaaagaac atatgcagga
     1261 ttaacggcag aattgcagcg gtatccatgc ttaaaaaaat cggggaaagg ctcatagatg
     1321 ttcacggaca gcatgacaac cagtcccttt taagaaccga gagtcatatt gagcttttgg
     1381 attctttcgc agatgacaaa attaatattc ttaaagatga gtatttaaag cattttgagt
     1441 cataccgcga gattaagaac aggttaaaaa gcctgaccgg cgatgccggt gaaagagagc
     1501 gaaaaattga tattctgaag tttcagatag atgagattaa gaaagccaag ctaaaggcag
     1561 gggaagagga agagattgag aagcaaagag aagttctttt aaattatgag aaaataatca
     1621 gcaccctctc caatgcatat gaggccttgg attccgggga gaaactcgga atatctgccc
     1681 tggacagaat aaataaatcg gtgagcgact tttgtaatat tgaagactat gacgagaaat
     1741 tcagtacatt aaaaaagaga cttgaagctg tatcttatga gcttgaggat attgtatcgg
     1801 agattcgcga tttaagggac ggtatagaat acgagccttc tgttttagaa caactcgagg
     1861 agaggctgga cgttttgtac cgcctgaaaa agaaatacgg ggactccata gagggcattc
     1921 tggaatatct ggagaatgcc aaaaaggaat tggatgagct ggtgaacaat gaggaaataa
     1981 taaacaggct gaaagaagac ctcaaaaggg aagatgaaaa gctgtataaa ttggcaaagg
     2041 atattaacag tgaacgggtt aagactgccg ggctgcttga aaatagaatc ggatcggagt
     2101 tttgtgacct ggaaatgaaa aataccaggc tcaaggtaaa tattgaattt gacgattcaa
     2161 tgaatgacgg tgagagaagg tataacaata atggacttga cagggttgag tttttgattt
     2221 ccaccaatgc cggggagccg ctaaaaccgt tggcgaagat agcatccggc ggagaaatgt
     2281 caagaattat gctggctatc aagaccattc ttgccaatgt cgataaaatg cctaccatga
     2341 tatttgatga aattgatatc ggaataagcg gtattgccgc acaaaaggtg ggagagaagc
     2401 tgtgttatat agcaaagaac catcaggtaa tatccgttac ccatctagct caaatagcat
     2461 gtatggcgga caataattat tatatagaaa aagtaacaaa agacggcagc acacagacag
     2521 ttgtctattg cttggatgaa gaaggaaaga gaaacgaaat agcaaggata atcggaggag
     2581 caaaggtttc cgatattgcc ttaaagcatg ccgaagaaat gctcgattat gccagagaat
     2641 tcaaaaaagc caacggaaaa ggctaaaaaa ccagtccatt gcagtataag tctattcctg
     2701 atttactgct tgtcagcaaa ataataatct taacataatt ctattgtaaa agtgtattga
     2761 accttaatat aaacattgcc ggaaagtgaa gaaaaacacg gaagaaactt aagatttctt
     2821 ctatattttt cttcactttc tttctatcgc aaagctttca atgtagtttt tgggtgaata
     2881 aataagatta ttataggtta acttaatgtt agccatgaat tgataaactc atggggtttt
     2941 tctttattct ataaatttta ttctttttga tttggaataa agttttattt ttttgcttta
     3001 aagtaaatca ggcatcagga ggtaattggt ttgaaatata taaaacccac taagagaaaa
     3061 gtaatccttt ttttagtgat atgctttgcg gttatgacta taacatattt gagaatgctg
     3121 ttttctcttc ccaatcaaat aacgcttttt gaaaacaagg aatacaatta caattttaaa
     3181 agtccttttc ttgtgaatct tgaagctgaa aacgatgaaa tattgatttt cgaaagcaag
     3241 gattccaagg tcagcaataa gaaaaacgga tttaacgaca gtatcatgat aaaggcaaaa
     3301 aagcttggga gaacaagcct gagcctgaga ttgttcgggc ttatacccat aaaaacaatg
     3361 caggtggatg ttgttccgta taaagaggtt atagcctgcg gaaatactgt gggagtgaag
     3421 ataaaagtag acggaattct tgtcataggt ttatcggatg ttgaaaccgt aaatggagaa
     3481 cgtttgttgc cggcaaggga ttcaggactc aaacccggtg a
//
LOCUS       DAOOJC010000256         3517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_20679_length_3517_cov_3.447429, whole genome shotgun sequence.
ACCESSION   DAOOJC010000256 DAOOJC010000000
VERSION     DAOOJC010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20679_length_3517_cov_3.447429"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="PLA01_07370"
     CDS             <1..1015
                     /locus_tag="PLA01_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPD01151.1"
                     /translation="AQQNKTGVTTASLLNVRQSPSTSTEVIDQIPRGGKVSIIETSNG
                     WHKVIYNNKTGWIYGGYVTITEPLPSNEAANNQTLVPGVESSKPDSSNTNPSSNSSSG
                     VVDETLLNSSNPSNINNQNTVIRTGTVQASILNVRSGAGTQNSIIGELARGETVNIVT
                     QKDGWYQIKLSNGSTGWVYSTYVIENTSVTRGGHDATIAVPDNTVDSNITDIRQQVVE
                     YGKKFLGTRYTYGGNTPAQGFDCSGFVKYVFTNFNINLNRVAADQAKQGTFVSKDSLL
                     PGDLVFFDTNGGHNYINHVGIYIGGGNFIHCSSGTNRGVVISELMTGFYATSYMTARR
                     VLK"
     gene            1093..3435
                     /locus_tag="PLA01_07375"
     CDS             1093..3435
                     /locus_tag="PLA01_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HPD01152.1"
                     /translation="MKRPLVLFCLSLIAGIVCTNITHSYLFAVLSCLVIALILLVLLN
                     NKKGSTFVLFGMVLFYITGAFYYLYTFNQNLYKFEDYAGKSVIIKGYIDSVPDIKESG
                     ITYILKVEEIEARGETYGKKKIQGKIQLSMQQGDTPVLLDYGREIIVSGKLNLPKGRT
                     NPGGFDYRSYLCHSGISAKLFAAERNIQPQDNVKGNLMVKAGLFIRDRIVRVINRSLP
                     PQQAGLLNGMLIGYREGLTEEVEDVFSKSGLTHIMAVSGANIAFIMLPLVFIFKKLRF
                     KKAVSNILIIGILIMFTFITGFEPSVLRAVIMAVVVLVGQIIMRETEIYTSIAFAAIV
                     LLLYNPGSLFSIGFQLSFAATISLVLFYKNIKNMIDFSFIPEFLVDVLASTLAAQVGV
                     LPITVFYFNKISIVSILSNLIVLPVVEFITILGALMAVLGQVHIGLSILLGYCNNALL
                     SFVLFATKVTAELPYSVVTVSTPSVILIIVYYIAVIYYLWYKPKHKVKLHFKYYALAG
                     TLTAALIMINLFLPKGMEVVFLDVGQGDCAFIKTQGGKNVLIDGGPDYAGENTVVPFL
                     LDYGISKLDLVVLTHGHDDHAKGVIPVFEYFAVDNIVMPDTVLEERLIGVYEIAQKKN
                     IPVEGCEKGDVINLDPKTYIEILHPKEGSYIEESPQNNNSLVLMLCYGDVKILFTGDI
                     EKQAEELLLKDITELDADVMKVAHHGSSTSSTEEFIESVNPAVAVISVGSNNFGHPSD
                     TVLDLFESKNIEVVRTDRCGAIVLKTRGGDIKLNTELKKR"
BASE COUNT         1055 a          613 c          805 g         1044 t
ORIGIN      
        1 tgcccaacaa aacaaaaccg gtgttactac ggcaagtttg ctcaatgtgc gacagtcacc
       61 aagtacttcc acagaggtta ttgatcaaat tcccagagga ggaaaggtta gtataattga
      121 aacctccaat ggctggcata aggtaattta caataataag acaggatgga tatatggtgg
      181 atatgttaca ataactgagc cgttgccatc aaatgaagca gcgaataatc agacccttgt
      241 tccgggagtt gaatcgtcaa aaccggattc ttcaaataca aacccttcca gcaatagttc
      301 ttcaggtgta gtagatgaaa cattattgaa ttcgtcaaac ccatcaaata taaacaatca
      361 aaacactgtt ataagaaccg gtactgttca agcttccata ttaaatgtca gaagtggggc
      421 agggactcaa aacagcataa tcggtgaact ggccagagga gaaacggtta atatagtaac
      481 ccaaaaggac ggctggtatc agattaagct gtcaaacggt tcgacaggat gggtatacag
      541 tacatatgtt attgaaaata catcagtaac aagaggaggg catgatgcca cgatagctgt
      601 gcctgataat actgtggaca gcaatattac cgatatacgc cagcaggtgg tggagtatgg
      661 aaagaaattt ttaggtacaa ggtatacata tggcggaaat actccagccc agggatttga
      721 ttgttctggc tttgtgaaat atgtatttac caattttaat atcaatctaa atagagtggc
      781 tgcagatcaa gcaaaacaag gaacctttgt ttcaaaggac agccttttgc ctggagattt
      841 ggtattcttc gataccaatg gagggcataa ttacataaac catgtgggaa tctatatcgg
      901 aggcggaaat tttatacact gctcgtcagg aaccaataga ggagtagtga ttagtgaact
      961 tatgaccgga ttttatgcca ccagctatat gaccgcaagg cgagttttaa aataattcaa
     1021 aagtgtttgc tttcacaaat acagctaagt aaggctcctt tgtgtaaagg ggcctttttt
     1081 gtgaggtgca taatgaaaag acccctggtg ttgttctgtt tgtctttaat tgccgggatt
     1141 gtttgtacaa atataaccca ttcttatctt ttcgcagtat tatcctgcct tgttattgct
     1201 ctgattttat tagttttgct taacaataaa aaaggcagta ctttcgtact ttttggaatg
     1261 gtactgtttt atattaccgg agctttttac tatctttata cttttaatca aaacctgtac
     1321 aagtttgaag attatgcagg gaaatccgtt attattaagg gttatattga ttctgtaccc
     1381 gatataaaag agtctggaat tacttatatt ttaaaggtag aggagataga agcaaggggt
     1441 gaaacctacg gaaaaaagaa aattcaggga aaaatacagt tgtctatgca acagggggac
     1501 actccggttc ttttggatta cggaagagaa ataatagttt cggggaagct gaatcttccc
     1561 aagggaagaa caaatccggg aggattcgac tatagaagct atttatgcca ttcgggtatt
     1621 tctgccaaat tatttgctgc cgaaaggaat attcaacccc aggacaatgt taaagggaac
     1681 ctgatggtca aagccggact ttttataaga gacaggatag taagggtaat taacagaagt
     1741 ctgccgccgc aacaggcagg gctcttaaac ggtatgctga ttggctacag ggagggactt
     1801 accgaagaag tggaagatgt tttcagtaag tcggggctaa ctcatattat ggcggtttca
     1861 ggggcaaata tcgcctttat catgcttcct ctggttttta tctttaagaa gctcaggttc
     1921 aaaaaggcgg tttccaatat actaatcata ggcattctga ttatgtttac ctttatcacc
     1981 ggttttgagc cttcggtgct gcgggcggta ataatggcag tagttgtgct cgtaggacaa
     2041 ataataatga gagagacgga gatttataca agcatagcct ttgctgcgat tgttttgctt
     2101 ttgtacaatc ccgggagcct ttttagcatt ggcttccagc tgtcgttcgc agcaacaatt
     2161 tctcttgttc tattctacaa aaatataaaa aacatgatag actttagctt tattcctgag
     2221 tttcttgtgg atgtattggc ttcaacactg gctgcacagg tcggtgtgtt acccattacg
     2281 gttttttatt tcaataaaat atcgattgta tcgatccttt cgaatcttat tgtattgcct
     2341 gtagtggagt ttataactat tctcggagct ttgatggcgg ttcttggaca ggtgcatatc
     2401 ggactttcaa ttctgttagg gtattgtaac aatgcactgt tgagttttgt cctttttgct
     2461 acaaaagtaa cggcggaact gccctattcc gttgtaaccg tgtccacacc ttctgtcata
     2521 cttatcattg tatattatat tgctgttatt tattacctgt ggtacaagcc gaaacataaa
     2581 gtaaagcttc attttaaata ttatgctttg gcagggactt taacagctgc tttgattatg
     2641 attaacctgt ttttgcccaa gggaatggag gtggtttttc tggatgtggg tcagggggat
     2701 tgtgccttta ttaaaaccca gggcgggaaa aacgtcttaa ttgacggtgg gcccgattac
     2761 gccggggaga acacggttgt gccgtttctg ttggattatg gcatatcgaa gcttgacctt
     2821 gttgtactga cccatggaca tgatgaccat gccaaaggag ttattccggt ttttgagtat
     2881 tttgccgtgg ataatatagt tatgcccgac acggtccttg aagagagact tattggtgtt
     2941 tatgaaattg cacagaaaaa gaatattccg gtagaaggct gtgaaaaggg ggatgttata
     3001 aaccttgacc caaaaactta tattgagatt ttgcacccta aagagggttc ttatattgaa
     3061 gagtcccctc aaaacaacaa ctctttagta ctcatgcttt gttatggaga tgttaaaata
     3121 ttgtttaccg gggatattga aaagcaagct gaggaattac tgctgaaaga cataacagag
     3181 cttgatgccg atgtaatgaa ggtggcacac catggttctt caacatcctc aacagaggag
     3241 tttatagaaa gtgtgaatcc tgcagttgcg gtaataagtg taggcagcaa caatttcggg
     3301 catccatccg atacggtttt agaccttttc gaaagtaaaa acatagaggt tgtgaggact
     3361 gaccggtgtg gtgccatagt actgaaaacc cgtggtgggg atattaaact caacaccgag
     3421 ctcaagaaac gttgacaagg ctcatataac atatgcgaag aaaaatatgg aagaaacatt
     3481 gtaaagcaag ttgcagccac ctgttaatgc gacgcgg
//
LOCUS       DAOOJC010000257         3514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_20697_length_3514_cov_4.689795, whole genome shotgun sequence.
ACCESSION   DAOOJC010000257 DAOOJC010000000
VERSION     DAOOJC010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20697_length_3514_cov_4.689795"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="PLA01_07380"
     CDS             complement(<1..1244)
                     /locus_tag="PLA01_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HPD01153.1"
                     /translation="MKNRKVLSLLLAIVILTTTCITFFTNITYAKTSRITLELDKTNA
                     DVGDIIEVAVKVENTTGLAAYQVNIKYDPKVLQAIDPVTGKAYNNRTIPDYGTVLVND
                     DYVPFPVAMNDIDMGIINFSGAYTDIDSYKASGVPEETGILGKIGFKVLKAEETSIKF
                     EGTERMPGAIEGTYLSDWDYKTLTDYKVEQPALINKKVEATPTTKPTSPTQKPTQTPT
                     IKPTATPKPTITVKPTPSPTKKPVNSYIEIDLNENEVQIGDIITATIRIKNISNLSAY
                     QLNVKYDPKMFRAVDPKTGKEYNNDTFPSGGTILVNDDYVPLTVVSNNIAKGIINFSK
                     SYLYLDEYKSSGKPEESGIICKIGFEALKIGKTSIKFAYTAEMLDSIQGTFLFDWDSK
                     KITGYSVYQPDEIKIVAKLPTAT"
     gene            complement(2004..>3514)
                     /locus_tag="PLA01_07385"
     CDS             complement(2004..>3514)
                     /locus_tag="PLA01_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014522595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPD01154.1"
                     /translation="TPTETEPTPTETEPTPTDTVPTPTETEPTPTETEPTPTETEPTP
                     TETEPTPTDTVPTPTETEPTPTETEPATIKVSVGEVEGYPGDIVLVPVTFEGVPAEGL
                     YKCSFELKYNSSVLDVDSILYGDILTQGDVTLTTRIEKEEGIIKIEFDAADLESINVS
                     GEFVVIKFVINKNAKAGKSSIEIVGSALFNGADGADINYSIANGGITIKEKQSEVTPT
                     ATPSATTKATPTPTPTNTSGGGGGGGGDFPTVYTPTPTVTPTVTPTPTSSTPPIITVP
                     PTIIPGPPLEGTHMSYLKGYPDGSFKPERNITRAEAAVIFAKLLGANENTDSQFTGSY
                     TDLKDNHWAAWAIKFTSRVGLFKGYPDGSFKPDQNITRAEFATVVLQFVIKVKGNAIQ
                     EKLANIDISKPKFDDVKGHWAQENIEKLTALGYISGYPDGTFKPENKIKRSESVALIN
                     RVLERGPLEEAPKTFPDVEEAYWAFKDIAEGALDHVYYIDPNELEIFIRILE"
BASE COUNT          927 a          717 c          583 g         1287 t
ORIGIN      
        1 gttgcagttg gcagttttgc aactattttt atctcatcag gctgatacac ggagtatccc
       61 gttatttttt tcgaatccca gtcaaacaag aaagtcccct gtatcgaatc cagcatttct
      121 gctgtataag caaacttgat tgatgtttta ccgatcttca atgcttcaaa ccctatcttg
      181 caaatgatcc cggattcttc aggttttccg cttgatttgt attcatcaag gtataaatat
      241 gactttgaaa agtttataat tcctttggca atattattgg atacaaccgt taacggtaca
      301 taatcatcat tcacaagaat agttccaccg gatggaaatg tatcattatt atattctttt
      361 cctgttttgg gatcaactgc cctaaacatt ttcggatcat acttaacatt aagttggtat
      421 gcacttaaat tactaatatt ttttatcctg attgttgcag taattatatc acctatttga
      481 acttcatttt cattcaagtc aatttcaatg tagctattta ctggcttttt tgtgggtgat
      541 ggtgtcggtt taactgtaat tgtcggttta ggtgttgcgg taggctttat tgtcggtgtt
      601 tgtgtaggtt tttgtgttgg tgacgttggt tttgtcgttg gagtcgcttc taccttttta
      661 tttattaaag caggctgctc tactttgtag tcagttaatg ttttataatc ccagtctgat
      721 aaataagttc cctcaatagc acccggcatc ctttccgtac cttcgaactt aatagaagtt
      781 tcttcagctt ttaatacctt aaaccctatt tttcccaaaa ttcctgtttc ctcgggcact
      841 ccgctcgctt tgtaactgtc gatatctgta taagcaccgc taaaatttat tattcccata
      901 tcaatgtcat tcattgcaac cggaaagggt acataatcgt catttacaag cacagttcca
      961 taatcaggaa tcgttctatt gttataagct tttcccgtaa caggatcaat cgcttgcaat
     1021 acttttggat catacttaat atttacttga tatgctgcta aacccgtagt gttttctact
     1081 ttaactgcga cttcaattat atcccccaca tctgcatttg ttttatccaa ttccaatgtt
     1141 attcttgaag tttttgcata tgtaatattt gtgaaaaatg ttatgcatgt tgttgttaat
     1201 ataacgattg ccaataacaa agataatacc ttcctgtttt tcatcaaatc ctccccctta
     1261 atttaaaacc taaagtttgt ttttcccata ctaaagtttt tttcccatat taatgtaata
     1321 tctgatcagg caacaccccc atataaatat ttatttcatt aattctattt aaattgaaga
     1381 gaatttatta attctctttt ttattgtcta atacagcact ttttctctga caccaattat
     1441 ataactaaaa taaataagct accatatcaa tacactaaac tttaatgaat attcaatata
     1501 ttcatccatt aacaacattt attgtcaaat tgatttttga atagcaagac aagggcagta
     1561 ccaggattag aagtcagcat aagtaaattc aataactgtg ataatgaatt ttgagaagta
     1621 taaaactcat attctctaaa taatacctaa aaaacccctc tctaaaacta ataatcattt
     1681 attagcaaaa gactaatgta ttaaataatt gtcttttgta atgtttcttg catcgtctat
     1741 atttgaccaa ttataaatat aattataatg taattttact aatatttcaa gctgttttgc
     1801 ttattaatgg atacttaatt taaatagttc atcaacgata attttttcta atttataaat
     1861 ttggatgttt caggaaggca tcgagcaccc atcgcttttg gtcatttcac ttcgtgaaat
     1921 cctactaata agaaagctgc tacgactgaa tttaattcgt agcagcttta gattatatta
     1981 tttcattttt acatcaacct taactattca aggattctta taaatatttc taactcgttc
     2041 gggtcaatgt agtatacatg atcaagtgca ccctctgcga tgtccttaaa ggcccagtat
     2101 gcttcttcca catcagggaa cgtcttcggt gcttcttcta acggacctct ctccagtact
     2161 ctgttaatta aggcaacact ttcagaacgt tttatcttat tctcaggctt aaatgtacca
     2221 tccggatatc cgctaatgta gcccaaagca gtcagcttct caatattttc ttgtgcccaa
     2281 tgacctttta catcatcaaa cttaggtttg ctaatatcta tatttgccag cttttcctgt
     2341 atagcattac cttttacttt tatcacgaac tgcagaacta cagtcgcaaa ctctgctctg
     2401 gtaatattct gatccggctt gaatgatcca tccggatacc ctttaaacag acccactctt
     2461 gatgtaaatt taattgccca tgcagcccaa tgattgtctt tcaaatcggt ataagatccc
     2521 gtaaattgtg aatctgtatt ttcattagca cctaataact tggcaaatat tacagcagcc
     2581 tctgctctag ttatgttcct ctcaggttta aagctaccgt caggatatcc ttttaagtaa
     2641 ctcatgtgtg ttccttccaa cggtggtcca ggaattattg ttggaggaac cgttattatc
     2701 ggtggtgttg acgatgttgg agtcggtgtc acagttggcg ttactgtcgg agttggggta
     2761 tatactgttg ggaaatcccc tccaccgccg ccaccgccgc cagaggtatt tgttggcgtc
     2821 ggtgtcggtg ttgctttagt agtcgccgat ggtgttgccg ttggtgttac ttctgattgt
     2881 ttttccttaa ttgttatgcc accgttagca atactatagt ttatatctgc accgtcagca
     2941 ccattgaaca acgctgaacc aactatttct atcgagctct ttcctgcttt tgcattttta
     3001 tttattacga atttgattac tacaaattca ccgcttacgt ttatactctc taagtcagca
     3061 gcatcaaatt caattttaat tataccttct tccttttcga ttctcgtagt gagggtaaca
     3121 tctccctgtg tgagaatatc cccatacagt atggaatcaa catccaatac gcttgaattg
     3181 tacttcaatt caaagctgca cttatagaga ccctctgccg gaaccccttc aaaggttaca
     3241 ggtacaagta caatatctcc agggtatcct tcgacttcgc caacagatac cttaattgtt
     3301 gccggctctg tttctgtcgg agtcggttct gtttctgtcg gtgttggtac tgtatccgtc
     3361 ggagtcggtt ctgtttccgt cggagtcggc tctgtttctg tcggagtcgg ctctgtttct
     3421 gtcggagtcg gctctgtttc cgtcggtgtt ggtactgtat ccgtcggagt cggctctgtt
     3481 tctgtcggag tcggctctgt ttctgtcgga gtcg
//
LOCUS       DAOOJC010000258         3494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_20839_length_3494_cov_3.046816, whole genome shotgun sequence.
ACCESSION   DAOOJC010000258 DAOOJC010000000
VERSION     DAOOJC010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20839_length_3494_cov_3.046816"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..1106)
                     /gene="spoIIGA"
                     /locus_tag="PLA01_07390"
     CDS             complement(213..1106)
                     /gene="spoIIGA"
                     /locus_tag="PLA01_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HPD01155.1"
                     /translation="MKIYIDVLVLENVVINYLILLLTAKFAKIKTSNLKLFLASLAGA
                     LYVVILLLFPNMKVYYSTTAKIALSFLIVAIAFSPRKLVSFIKTMVIFYVSTFIFAGA
                     AFAFFYFNQSGGFVKNGMVYVWNSKWTSLFFAILTAGIIIRIFWDIVQQRLSKEKLLI
                     PLKIAFENEITDMSALIDTGNSLYDPLTNSPVVVVEFNAIKHILPMEIQSIFDKSIED
                     DLTSVTRIVSDSSWLSRFRLIPFKSLGKENGMLIGFKPDYIEVGENSEKKGVTDVIIG
                     IYNRALSKNNNYNALLNLELV"
     gene            complement(1513..2607)
                     /gene="ftsZ"
                     /locus_tag="PLA01_07395"
     CDS             complement(1513..2607)
                     /gene="ftsZ"
                     /locus_tag="PLA01_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPD01156.1"
                     /translation="MLEFDIDMEQFAQIRVVGVGGGGNNAVNRMIAAGLRGVEFIAIN
                     TDKQALFLSKANTKIQIGDKLTKGLGAGANPEIGEKAANESKDEIAQAIKGADMVFVT
                     AGMGGGTGTGAAPVVAQIAKEMGILTVGVVTKPFMFEGRKRMQHAERGIETLKTTVDT
                     LVTIPNDRLLQVAEKKTSIVDAFKIADDVLRQGVQGISDLIAVPGLVNLDFADVKTIM
                     LDTGLAHMGIGRASGDNRAEEAAKQAILSPLLETSIEGARGVLLNITGGADLGLFEVN
                     TAAELVQKSADPDANIIFGAVIDENLKDEILITVIATGFDRAPVAKKIDKPVVAERTP
                     VSTPEKTAPSVQTPSDELEIPTFLRRNRFK"
     gene            complement(2895..>3494)
                     /locus_tag="PLA01_07400"
     CDS             complement(2895..>3494)
                     /locus_tag="PLA01_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HPD01157.1"
                     /translation="GVTNVSVFKNKSIVLYDSVPVGGDHITNDISIGLRISSGDADKL
                     KREYGLALTSLISNDHEISVNELDGNTKKTIKVSQVVEIIEARIQEIFFLCKEKLEET
                     GEFSDFSGEIVLAGGGISYMDGSVELAKEVFGLPARLVAYRPLGITNPEYLTSIAILR
                     YVADRMKSGRKDTKSTKPNKHKKEFGILKRIAGLFNGLF"
BASE COUNT         1084 a          794 c          521 g         1095 t
ORIGIN      
        1 ttcctcaata catttatgta cttaatcaaa aaaaagaact taatcctctt taaaaaaaac
       61 ataacctctg ctcccttcaa ttcgaaatat tgatagccag ttttatcctg atttcccggt
      121 cctatcttac aaaatacagc ttttcttttt acaagatttc agagatacta cgcagtattt
      181 caggtcctac cttacaaaat agagcttatc ttctaaacca gttccagatt taaaagagca
      241 ttataattat tattcttgga taacgccctg ttgtatattc cgataatcac atctgtaacc
      301 cccttctttt ccgaattctc ccctacctca atataatcag gcttaaaccc aattaacatg
      361 ccgttttctt tgcccaagga tttaaacggt ataagtctga acctcgacag ccaactggaa
      421 tcggatacaa ttctggttac gctggtcaga tcatcttcaa ttgatttgtc aaagatgctc
      481 tgtatttcca taggcaatat atgtttaatg gcattaaact ctacaaccac aaccggtgag
      541 ttggttaatg gatcataaag ggagtttcct gtgtcgatca gagctgacat atcggttatt
      601 tcattttcaa acgcaatttt taacggtatc agaagctttt ccttcgaaag cctttgttga
      661 actatatccc agaaaatcct tataataatc cctgctgtta aaattgcaaa aaaaagcgag
      721 gtccattttg aattccaaac atacaccatt ccatttttta caaagccccc tgattggttg
      781 aaataaaaaa atgcaaaggc cgcacctgca aatataaatg ttgaaacata aaatatcacc
      841 atagtcttga taaacgatac taacttccga ggagaaaaag caatagctac aatcaaaaat
      901 gacagagcta ttttcgcagt agtactgtag tataccttca tgttgggaaa caacagcagg
      961 attactacat acagagcacc tgccaaagaa gccaaaaaca gctttaaatt agaagtctta
     1021 atctttgcaa attttgcagt tagcaacagt ataaggtagt ttataactac attctccaga
     1081 accaaaacat caatatatat tttcattctg tcgcctcctc acaaattcca caaacaaata
     1141 aatgctccta ccacaagaaa atatcctcaa taacatatat gcaagagcat gtttttttat
     1201 tattgattat acagataaat atatatttat tttgtcaaac ctcagtgtag aaaaaagaaa
     1261 tcgtgtgaca aaatcaattg agttttttag gtaggggaga agttatgaaa aatatttcag
     1321 aaacttgagt tccgcagtct agagcctgac ttcacaatgt ttctttcata tttttcataa
     1381 cttgtcgtcc ggttgaggca ggtatataaa gattaatata cttcaacaga taataaaaaa
     1441 gtgcaagagc atatgacaaa gaaactctta cactttatca gccgttatta attctttaat
     1501 tactcaaaat aattacttga atctgtttct ccttaagaag gttggtattt ccaactcgtc
     1561 cgacggcgtc tggacagaag gagcagtctt ctctggagtt gatacaggtg tcctttcggc
     1621 aacaacgggc ttgtctattt tctttgctac aggagctctg tcaaaacctg tggctattac
     1681 ggtaattaaa atctcatcct taagattttc atcaataacc gctccgaata taatattcgc
     1741 atcggggtcg gcagattttt gcaccagttc tgctgccgta tttacttcga acagtccaag
     1801 gtcggcaccg cccgtaatgt taagaagaac ccctcttgct ccctcaatag aagtctctaa
     1861 aagaggactc aaaattgcct gttttgcagc ctcctcagcc ctgttatcac cggaagcccg
     1921 tccaataccc atatgagcca gcccggtatc cagcattatt gtcttgacat cagcaaagtc
     1981 aaggtttaca agacccggta cggcaatcag atccgatatt ccctgtacac cttgccttaa
     2041 aacgtcatcg gcaatcttaa aggcatcaac gatggaagtc ttcttttccg ccacctgaag
     2101 aagcctgtca ttgggtatcg taaccagagt atcaacagta gttttcagag tctctatccc
     2161 tctttctgca tgctgcatcc tctttcttcc ttcaaacata aagggtttgg tcacaacgcc
     2221 aacagttaaa atacccattt ccttagcaat ctgtgctacc accggagcag ctcccgttcc
     2281 tgttcctcca cccataccgg cagtcacaaa aaccatatct gcacccttaa tcgcttgagc
     2341 tatttcatcc ttgctctcgt tagcagcctt ttctccaatc tcaggattgg caccggcccc
     2401 aagtcctttt gttaatttgt ccccaatttg gatcttagta tttgcttttg ataaaaacaa
     2461 agcctgttta tccgtattaa ttgctataaa ttccacacca cgtaaacctg cagctatcat
     2521 tctattaaca gcattatttc ctccaccgcc aacacctaca actcttatct gggcaaactg
     2581 ttccatatcg atatcaaact ccagcaattt aaatccccct ttttttcatt ggtaataaac
     2641 caaccttgat taaatcactt ctatgctgat tttcaagata ttgttccaaa ttaaatatac
     2701 atgctcacaa tcattctctc aataattaat aatatattaa tattcgagat ttcgcaagaa
     2761 cgtatttcgg gtatttcatc ctatattcaa aatctaaaat atacatcggt aagaacgaaa
     2821 aaacaattat atttattatt tataatagta ttcaataata attaaaatat gtatcgaaaa
     2881 aaagaactaa aatgttaaaa taaaccatta aagagaccag ctatcctttt tagaataccg
     2941 aattcctttt tatgcttgtt tggtttcgtg ctttttgtgt ctttcctccc tgacttcatt
     3001 ctgtctgcaa catacctcaa aatagcaatt gaagtcaggt attccgggtt agtaataccc
     3061 agtggcctat aggctaccaa ccttgcaggc aaaccaaaca cttcctttgc caactcaaca
     3121 ctgccgtcca tatatgatat accgcctcca gcaagaacaa tctctccgct gaagtcactg
     3181 aattcgccgg tttcttcaag tttttccttg cacagaaaaa atatttcctg tatcctcgcc
     3241 tcaattattt cgaccacctg ggaaaccttg atggtttttt tggtatttcc atcaagctca
     3301 tttaccgata tttcatgatc attactgata agtgatgtaa gggccaagcc atactctctc
     3361 tttagcttgt ctgcatcgcc cgatgatatt ctaagcccta tggaaatatc atttgttata
     3421 tggtcccctc ctacaggaac ggaatcatac aaaactatgc tcttgttctt gaatacagat
     3481 acattggtaa cccc
//
LOCUS       DAOOJC010000259         3469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_20999_length_3469_cov_3.161101, whole genome shotgun sequence.
ACCESSION   DAOOJC010000259 DAOOJC010000000
VERSION     DAOOJC010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20999_length_3469_cov_3.161101"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..665)
                     /locus_tag="PLA01_07405"
     CDS             complement(162..665)
                     /locus_tag="PLA01_07405"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPD01158.1"
                     /translation="MHDILKEKKDSLYGKPPRLSQVEMTELVLPNDTNLLGNLLGGRL
                     MHWIDVAGAMAASRHSNKVVATVALDSLDFRHPARMGELVMLKAKLTWVGRTSMEVCV
                     KAYAENLKTGDVIKTNQAFITFVALDGEGNPAPVPPLILETDEEKQMFKEAEIRRAER
                     LKRKEGN"
     gene            complement(855..1964)
                     /gene="pilM"
                     /locus_tag="PLA01_07410"
     CDS             complement(855..1964)
                     /gene="pilM"
                     /locus_tag="PLA01_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HPD01159.1"
                     /translation="MVTIPFIKTNLISIDIGFRNIKIVEVELGRNNEIFIKNFGIVAT
                     PKGCIKNGAIKDVKKVTSEIKRIMGDINTRAKNAKIVMSGTNIISRIFVIDKIPGEDL
                     NHLVRTTISQSMPIDLDAHKIDFKILKEFREDGINKVKVFVTAVLKSIIQSYIDILVG
                     LGLKPVSVDIPANSTAKFFNREIMVSESETWFKKKRYSKLSQNTFAVIDFGSETTIVN
                     ILRDKVLEFNKVILRGSSNLDEAIATNTQKEIDEAERLKKIQGLTLPEMNVGDTQEVI
                     YRSIKSVVDDIVRQIFQCFEFYEKRCYGEKVGKIYMIGGGSLLKGLREYLEEIFQVPV
                     YPVGLLSIEGIQINKGLDGERLNFLINSVGISLGN"
     gene            complement(2080..3063)
                     /gene="dusB"
                     /locus_tag="PLA01_07415"
     CDS             complement(2080..3063)
                     /gene="dusB"
                     /locus_tag="PLA01_07415"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPD01160.1"
                     /translation="MKIGSVSLDNNVFLAPMAGITDIPFRLLCKEQGCGLVYTEMVSA
                     KGIYYNDEKSKKLTMTDRREGPVALQIFGSDPGIMAKVAESLNESSACIVDINMGCPT
                     PKITKNGEGSALMLKPELVGRIVKEVTRASVKPVTVKIRKGWDDNRVNAVEIAKIAQE
                     NGASAVAVHGRTREQFYSGKADWSIIRQVKEAVSIPVIGNGDIFTPNDAIKMIDETKC
                     DAVMIGRGAQGNPWVFNNVINSFNKTGALTELSVRDKIDMIIRHMSMLIDLKGEQRGI
                     REMRKHIAWYIKGMPNSAHVKEKVFKAEYYTEVVDILSSFLHCNNVIKYSI"
BASE COUNT          991 a          803 c          495 g         1180 t
ORIGIN      
        1 ccgtggctag actttcacta gctagattag tgccatgcct ggcacacagc ataaagccgg
       61 ctttttgcaa aaatccaaag gccggcttta actttactaa aaccttaatt aacggtgaag
      121 aaaatatgga agaaacattg tgaagcaagt tatagagcct gttaattccc ttcttttctt
      181 ttaagtcgct ctgctctcct tatctcggcc tctttaaaca tttgcttttc ctcatcggtc
      241 tcaagaataa gcggtggaac cggtgccgga tttccttcac cgtccaatgc tacaaaggtg
      301 ataaatgcct ggtttgtttt aattacatca ccggtcttta aattttccgc gtatgctttt
      361 acgcaaacct ccatggaagt tctgccaacc catgtaagct tcgccttaag cattactaat
      421 tctcccattc gggcaggatg tctgaaatcc aagctgtcca atgcaacggt tgccacaacc
      481 ttgttcgaat gtcttgaagc cgccatagct cctgcaacat ctatccagtg catgagcctc
      541 cctccaagaa gatttcccaa aagattggta tcattgggaa gtaccaattc tgtcatttcc
      601 acctgggata atctcggtgg ttttccgtaa aggctgtcct ttttctcttt taaaatatca
      661 tgcatattct aatttcccct tttctatcca ttttctataa ctctgctatc tgcaatcgga
      721 taaaatccca ctaacaaaag ctcctgggca tcgagcccat tcgctttttg gtcatttcac
      781 ttcgtgaaat cccactaaat caaaaaacgg caccttccat gccgttataa ttacctatta
      841 atataaattc tatatcagtt acccagggaa ataccaacgg agtttatcag gaagtttaat
      901 ctttcaccat caagtccctt atttatctga attccttcta tgctaaggag gccaacaggg
      961 tataccggaa cctgaaatat ctcttcgaga tattcacgaa gccctttaag cagcgagcct
     1021 ccgcctatca tgtatatttt gccgactttt tccccgtaac acctcttctc ataaaattca
     1081 aagcattgaa agatctgtct cacaatatcg tcaacaacac ttttaatgct tctgtatatt
     1141 acctcctgtg tatctcccac attcatctcc ggaagagtaa gtccctgaat ctttttgagt
     1201 ctttcagctt catcaatctc tttttgcgtg ttggttgcaa tagcctcgtc cagattactg
     1261 cttcctctca aaataacctt gttaaattca agtactttat ccctcaaaat atttactatt
     1321 gtggtttccg atccgaaatc aataaccgca aaggtgttct gactgagctt gctatacctc
     1381 tttttcttaa accaggtttc gctttctgaa accattattt ccctgttaaa aaactttgcg
     1441 gtactgtttg ccggaatatc caccgataca ggttttaacc ccaggccaac caatatatct
     1501 atatagcttt gaataatgct ctttaaaact gcggtaacaa aaaccttaac cttattaatt
     1561 ccatcctcgc ggaattcttt cagtatttta aaatctattt tatgtgcatc aagatctatc
     1621 ggcatactct gcgaaatagt ggtccttaca agatggttca aatcttcacc gggaatttta
     1681 tcaattacga atatacggga aataatattg gtacctgaca tgacaatctt ggcattttta
     1741 gctctggtat ttatatcccc cattatcctt ttaatctcac tggtcacttt ctttacatct
     1801 ttaatggcac cattttttat acaccctttc ggtgtagcca ctataccgaa attcttgata
     1861 aaaatctcat tatttctccc cagttccact tcaactattt taatatttct gaagccgata
     1921 tcaatactga taaggttggt tttaatgaaa ggtattgtca ccattttatc tactccttaa
     1981 gcattattgg gatgacctat atgtaaatat tatcatccat gcattttaaa atcaacaatt
     2041 agtgtcaatt aatattaaaa aaatgtaaac atactatttt tatatgctgt attttatgac
     2101 attattacaa tgaaggaatg aactgagaat gtcaacaact tctgtgtaat actccgcttt
     2161 aaatactttc tcctttacat gggctgagtt tggcataccc ttaatatacc acgcgatatg
     2221 ttttctcatt tcccttatcc ctctctgttc gcccttcaaa tcaataagca tactcatatg
     2281 cctaataatc atatctatct tatctctaac cgataattcg gttagtgccc ccgtcttgtt
     2341 aaaagaattg atgacattat taaaaaccca tggatttcct tgggctcctc ttccaatcat
     2401 tacagcatcg cattttgttt catcaatcat ttttattgca tcgtttggtg tgaaaatatc
     2461 tccgttccct atgacaggta tgcttacagc ttctttaacc tgtctgatta tgctccaatc
     2521 cgctttaccg ctgtaaaact gttcccgagt tctcccatga actgctactg cagaagcccc
     2581 gttttcctgt gcaattttag ctatttcaac agcattaacc ctattatcgt cccaaccttt
     2641 tcgtatcttt acagtaacgg gctttaccga agccctggtt acctctttga ctatccttcc
     2701 cacaagttcc ggcttaagca ttaatgcact gccttcaccg tttttcgtta ttttgggtgt
     2761 gggacatccc atatttatgt caactatgca tgcacttgat tcattcagac tttctgccac
     2821 ctttgccata atacccggat ccgaaccgaa aatctgcaaa gctacaggtc cctctcttct
     2881 gtctgtcatt gtaagctttt tgctcttctc atcattatag taaatgcctt ttgcacttac
     2941 catctccgta tatacaagtc cgcatccctg ctccttgcat aaaagcctga aaggtatgtc
     3001 ggttatacct gccataggag ctagaaaaac gttgttatct aagctgacac ttcctatttt
     3061 catgacaacc tcctactata tatattctat cacgaatatc aaaataaaaa aacaacaaat
     3121 tgtataaaaa attaaatatt tgatcagtaa acatagtata accatgtcta aagcaaaaat
     3181 aacagtcctc ctaatgtccc attgtcttca tataaattgc aagattcgga tgccgatatt
     3241 tctttttccc aaaaaatcca aaaaagtaat cctcgactta aatcaaaaaa tgtagtataa
     3301 tattaatata gcattatatt tacaaacata aagtaataag caaaatctcc taaacttttt
     3361 atcactgcgt aattaattca cttctgtgct ttattaatat taagccactg aaagttatga
     3421 ttgacagctc ataaaatagt attgacaatt attattaagg aattgtttt
//
LOCUS       DAOOJC010000260         3444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_21181_length_3444_cov_3.088227, whole genome shotgun sequence.
ACCESSION   DAOOJC010000260 DAOOJC010000000
VERSION     DAOOJC010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21181_length_3444_cov_3.088227"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PLA01_07420"
     CDS             <1..701
                     /locus_tag="PLA01_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HPD01161.1"
                     /translation="AIGFIKGIKSDVLVLVDNCYGEFVESLEPIEVGADLAAGSLIKN
                     PGGGIAPTGGYVVGKRSCVQKAAYRLTTPGLGKKVGSSLDNNRLMYQGLFLAPHVVAE
                     SLKGAVFCAAVMKELGYSTSPQVHEKRGDIIQAIRFDNPDSLIAFCQGIQKGSPVDSF
                     VTPEPWDMPGYESPVIMAAGAFIQGSSIELSADAPIKPPYIAYMQGGLVFEHVKLGVM
                     IAIQKMLEKGKIQI"
     gene            711..2054
                     /locus_tag="PLA01_07425"
     CDS             711..2054
                     /locus_tag="PLA01_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPD01162.1"
                     /translation="MKDLEEINERKKSESETNDRRKGESGITGRIKLGGIIILLFLLL
                     YIPSFVFWVYSKNISVDILRMGEIEESIPVDALIVRNETVVNSPADGIIIKDIEEGEK
                     IRAGSTIATILNKSSEKLLDDLKALDLRIIEAQREKAKNDGFFVEDIKNIDGQIEDKL
                     MIVMAQASSNSISEVKNIKKDIDGLIQKKATITGDLSISDAHINSLKAQKQVLQESIN
                     QNKSNIVSDIPGIISYVVDGCESFLNQNAISSLTSKDIKSIKPQNPVKKMDDLGVEFN
                     KPVVKVISGIDYYIVFAVDKKEAEGFKVEDSWNLRINDINRTIDGTVMYKSDETDGKV
                     VIAVRTDKAMSDTAALRNINVDLIKNRYSGLVVPLKCLIDIDMTKMTAKIGLVRAQRA
                     EFVPVKIVGINNEFAIIDNVDSSYYTGDNSSRTTISLYSSYIVNPKNIEEGQIVN"
     gene            2054..2761
                     /locus_tag="PLA01_07430"
     CDS             2054..2761
                     /locus_tag="PLA01_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPD01163.1"
                     /translation="MDEKLDFIRRNLDAVMERIEMAAQRSGRSAKDIKLVAVTKTVEP
                     ERILKIIDEGILDLGENRVQELTKKYDIINRECNWHLIGHLQTNKVKYIMGKVKLIHS
                     VDRIELVREINKRAQSQGITADVLVQVNVSGEESKFGVPLEEAHELVEQMSILSNLKI
                     RGFMTIAPFAENPETIRYVFSKLRDFSIDIRKEKLDNSNMDILSMGMSNDFEVAIEEG
                     SNMVRIGTALFGQRNYT"
     gene            2858..3316
                     /locus_tag="PLA01_07435"
     CDS             2858..3316
                     /locus_tag="PLA01_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HPD01164.1"
                     /translation="MSKIFNKVLNFVGWETEDEEEMENVENLNEKEEVEKPQFIQSIS
                     KKTQNKVVNIHSTSQFKLIVTQPQDFNDAKDVCDHLKNKKPVVVNLEGIQKETAQRIV
                     DFLSGSVYALDGNIQKVSNGIFIVAPCNVDIMGDFQEEITTNKAVFPWMR"
BASE COUNT         1173 a          505 c          830 g          936 t
ORIGIN      
        1 aggctatagg ttttataaag ggaataaaaa gtgatgtatt ggttcttgtg gacaattgct
       61 acggagaatt tgttgaaagc ctggagccta ttgaggttgg ggcggatctg gcagcaggtt
      121 cgcttatcaa aaaccccggt gggggtatcg ctcctacagg tgggtatgtt gtgggaaaga
      181 ggtcatgtgt ccaaaaggcg gcctataggc ttacgactcc gggcttgggc aaaaaagtgg
      241 gttcttcttt ggataataac aggcttatgt accagggact gtttttggca ccccatgttg
      301 ttgctgaaag tcttaaagga gcggtattct gtgctgcagt tatgaaggaa ctgggctaca
      361 gtacaagtcc gcaggtgcat gagaaaaggg gagacattat tcaggcaatt agatttgata
      421 accctgacag cttgattgcg ttttgccagg gaattcaaaa gggttcacct gtagattcct
      481 ttgtcactcc ggaaccgtgg gacatgccgg ggtatgaaag tcctgtgata atggctgccg
      541 gggcgtttat tcaggggtcg tccattgagc ttagtgcgga tgctcccatt aagccgccgt
      601 atatagctta tatgcaaggc ggattggttt ttgaacacgt taagcttggc gttatgatag
      661 ccatacaaaa gatgcttgaa aagggaaaaa tacaaatctg atgtttgagg gtgaaggatt
      721 tggaagagat aaacgaaaga aaaaagagcg aaagtgaaac aaacgacaga agaaaagggg
      781 aaagtggaat aaccggaaga ataaagctgg gaggtataat tatacttctg tttttgcttt
      841 tgtatatccc atcctttgtt ttttgggttt acagcaaaaa catttccgtt gatatattga
      901 ggatgggcga gattgaagaa tccattcctg ttgatgcttt gattgtcaga aatgagactg
      961 tggttaattc acctgccgac ggaataatta taaaggatat tgaagaaggt gaaaaaatca
     1021 gggctggcag cacaatagcc acaatactca acaaatcatc ggagaaactt ttggatgatt
     1081 tgaaggcact ggatttgcga attatagaag cccaaaggga aaaggcaaaa aatgatggct
     1141 tttttgtaga agacataaaa aatattgacg ggcaaataga agataagctg atgattgtta
     1201 tggcacaggc tagtagcaac agtatctcgg aagttaaaaa cataaagaag gatattgacg
     1261 ggcttatcca aaagaaagca accataacgg gagatctgag tatttccgat gcccatatta
     1321 attctctcaa ggctcaaaag caggtgctgc aagaaagcat aaaccaaaat aaaagtaata
     1381 ttgtttcaga tatcccggga ataatatcct atgtggtgga tgggtgcgaa agctttctca
     1441 atcaaaatgc aataagcagt ttaacctcaa aagacataaa aagcataaaa cctcaaaacc
     1501 ctgtaaagaa aatggatgat ctgggtgttg agttcaacaa acccgttgtt aaggtgataa
     1561 gcggcataga ttattatatt gtttttgccg ttgacaaaaa ggaggccgag gggtttaagg
     1621 ttgaagacag ttggaattta aggattaacg atatcaacag aactattgac ggaacagtta
     1681 tgtacaaatc cgatgagaca gacggaaaag tcgttatagc tgtgaggacg gataaggcta
     1741 tgagcgatac ggcagcctta aggaatatca atgtggattt gataaaaaac cgatacagtg
     1801 ggcttgtagt gcctttgaag tgccttattg atattgatat gacgaaaatg acagctaaaa
     1861 tcggattggt cagggcacag cgtgccgaat ttgttcctgt taagattgtc ggaataaaca
     1921 atgagtttgc tataatagac aatgtcgata gtagctatta tacgggcgat aattcttcgc
     1981 gtacaactat aagtttgtat tcgagttata tagttaatcc aaaaaatatt gaagaagggc
     2041 agattgtaaa ttgatggacg aaaaacttga tttcataaga agaaaccttg atgccgtaat
     2101 ggaaagaata gaaatggcag cccaaagaag cggaagaagt gctaaggaca tcaaacttgt
     2161 tgcagtaaca aaaacagttg aaccggaaag gattctaaaa attatcgatg aaggaatttt
     2221 ggatctggga gagaataggg ttcaggaatt gactaaaaaa tacgatataa taaacaggga
     2281 atgcaattgg cacctgattg ggcatcttca gacaaacaag gtaaaatata ttatgggcaa
     2341 ggtcaaatta attcattcgg tcgacaggat tgaactggtc cgggaaataa ataaaagggc
     2401 acagagccaa ggaataacag cagatgtatt ggtccaggta aatgtttcgg gagaggagag
     2461 caagttcggt gttcctttgg aagaagccca tgaactggtt gaacaaatga gtattctttc
     2521 caatttaaag atcagaggct tcatgaccat tgcacctttt gctgaaaatc ctgagacaat
     2581 tcgctatgtt tttagtaaat taagggactt ttctattgac ataaggaaag aaaaacttga
     2641 taatagtaat atggatattt tgtccatggg tatgagcaac gattttgagg ttgcaataga
     2701 agaaggttca aacatggttc gtataggcac tgccttgttt ggccaaagaa attatacata
     2761 atcggagcaa tatcttctaa gaggtgtttt ataaaacagc cattttagaa gaattgtata
     2821 tagttatttt acaaaagata aacaggaggg atttactatg tcaaaaatat ttaacaaggt
     2881 tttgaacttt gtaggttggg aaacggaaga tgaggaagaa atggagaatg ttgaaaactt
     2941 gaatgagaaa gaagaagttg aaaagcctca atttatccaa tctataagca agaaaactca
     3001 aaacaaggtt gtaaacattc attcaacatc tcagtttaag ttaattgtta cgcagccaca
     3061 agactttaat gatgccaagg atgtatgtga ccatttgaaa aacaagaaac ctgtagttgt
     3121 aaatcttgag ggcattcaaa aggagactgc tcaaagaata gtagactttt taagcggatc
     3181 cgtttatgcc cttgacggca acatccaaaa ggtttcaaac ggcattttta ttgttgctcc
     3241 atgtaatgta gatattatgg gagatttcca ggaagaaata accactaata aagctgtttt
     3301 tccttggatg agatagttgg agggtataaa tgaatggtgc taacctgaca attaaagcat
     3361 tgcaagcatt tgacctgtta ttgaatataa ttatgtggtc aattatattg cgtgctttat
     3421 tatcatggtt tccgatatca aaag
//
LOCUS       DAOOJC010000261         3422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_21341_length_3422_cov_3.536680, whole genome shotgun sequence.
ACCESSION   DAOOJC010000261 DAOOJC010000000
VERSION     DAOOJC010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21341_length_3422_cov_3.536680"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLA01_07440"
     CDS             complement(<1..187)
                     /locus_tag="PLA01_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01165.1"
                     /translation="MGLNLAQKIIKEHLISGDMKAGTEISIRIDQTLTQDSTGTMAYL
                     QFEAMGIPRVKTKKSVAY"
     gene            complement(212..1591)
                     /locus_tag="PLA01_07445"
     CDS             complement(212..1591)
                     /locus_tag="PLA01_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPD01166.1"
                     /translation="MIEFNKRTNTLEQNQYKYPLQDVSEPNLYRDIFNYDEIPKCAFN
                     HRKVPMFPADEIWITDTTFRDGQQSRAPYTVDQILHLFDLLHQLGGPKGIIRQCEFFL
                     YSDRDKEAVYKCMERGYEYPEVTSWIRATKNDFQLAKDMGVKESGILVSCSDYHIFKK
                     LNMTRKQALDHYLNIVKSAIEVGIKPRCHFEDITRADFYGFVVPFALELQKLMEESGV
                     PIKIRACDTLGYGVSYPGVALPRSVPGIIYGLRHHASFPSELLEWHGHNDFYKAVSNS
                     AAAWLYGASSVNCSLLGIGERTGNTPLEAMVIEYAQLRGTTDGMDTTVITEIAEYYQK
                     ELGYEIPTRTPFVGRHFNVTQAGIHADGLLKDEEIYNIFDTSKILNRPVGVAINQSSG
                     LAGIAHWINSFFGLEGSKKIDKKDERVVKIKEWVDEQYKTGRVTAIGDSELEEIVRKI
                     APDIFDLAL"
     gene            2047..3339
                     /locus_tag="PLA01_07450"
     CDS             2047..3339
                     /locus_tag="PLA01_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPD01167.1"
                     /translation="MKKSVFGFKPLLGLFLLIAIAVMSFIASLLAQRWQAAISVIVSN
                     IVILSIVSIVFYAKFKKVAKEFEKEVDLVKQEGFSKTINTEELYMLQSTAHVVNGLLS
                     DIKALINNFHNLSRSIIDATKSVSSTAQQASIAMEDIANTMDDIASGASDQAEQAQQG
                     VEMVDNLSEQINFVYESYNRITEETKKINDLNNIGLDSVSILRDKSKENYETAEKIFS
                     VVEKLTNTIKDIGLFVESIENIAEQTNLLALNAAIEAARAGEAGKGFAVVAEEVRKLA
                     DQSRKSTEEINILMQSIHEESQLSIKSMETMKRVSQEQNVAVNKTDSAFNDIANAITY
                     IVSKINEVNQAVTKMQTDKIEVTSAIENISSVSQETAAASQQVAATTEHELKAIEAMN
                     ESAKKLEQLVEELDSKLKKNAHFIAGDENDQPPTIETF"
BASE COUNT         1026 a          710 c          639 g         1047 t
ORIGIN      
        1 tatatgccac cgactttttg gtctttaccc ttggaattcc cattgcctca aactgcagat
       61 atgccatggt tcccgtagaa tcctgagtca aagtctgatc tatccttatg gatatttccg
      121 tgcctgcctt catatctccg cttatcagat gttctttaat aattttctgt gctaagttta
      181 aacccatttg tgattcctcc ccgtttatta tttaaagtgc cagatcaaat atatccggtg
      241 ctatttttct tacaatctct tccagctcac tatcccctat agctgtaacc cgtccggttt
      301 tatattgttc atccacccat tccttgatct tgaccactct ctcgtctttt ttgtcgattt
      361 tcttggagcc ctccagtccg aaaaagctgt taatccaatg tgcaattccg gcaagacctg
      421 atgattggtt tattgcaaca ccgacagggc ggttcaaaat cttagaggta tcaaatatat
      481 tgtaaatctc ctcatccttc aaaagaccgt cagcgtgtat tcccgcctgc gttacattga
      541 aatgccttcc aacaaacggt gtcctggtgg gaatctcata cccgagttcc ttttgatagt
      601 actcagcgat ttcagttata acggttgtgt ccattccgtc ggtggttccc ctcagctgtg
      661 catactctat aaccattgcc tctaaaggtg tgtttcctgt cctttcacct ataccaagga
      721 gtgaacagtt aacagaagag gctccgtaaa gccatgctgc agcagaattg cttaccgctt
      781 tgtagaagtc attgtgtccg tgccactcta aaagctcgct ggggaaactt gcatggtgcc
      841 tgagtccata tattattccc ggtacacttc gtggaagtgc cactccggga taagatactc
      901 cataacccaa tgtatcgcag gcacgtatct taatcggaac tcccgactcc tccatgagct
      961 tctgcagttc aagagcaaag ggaacaacaa agccatagaa atctgccctt gtaatgtcct
     1021 caaaatgaca tctgggtttt atgcccacct caatagcact ctttactata tttaaataat
     1081 ggtccaatgc ctgttttctt gtcatattta gcttcttaaa tatatgataa tccgagcagc
     1141 tcaccaaaat accgctttcc ttaaccccca tatccttagc gagctggaaa tcattttttg
     1201 ttgctcttat ccagcttgtg acctccgggt attcatagcc tctttccatg cacttataaa
     1261 ccgcctcttt atccctgtca ctgtacaaga aaaactcgca ctgccttatt ataccttttg
     1321 gtccgcctaa ttggtgcaaa agatcaaaaa ggtgcaaaat ctgatccaca gtataaggtg
     1381 ccctggactg ctgaccatcc ctgaatgtag tgtcggttat ccagatttcg tcggcaggaa
     1441 acataggtac ttttctgtga ttgaatgcac acttgggtat ttcgtcataa ttgaaaatat
     1501 ccctgtaaag atttggttcc gatacatcct gaagcgggta tttgtactgg ttctgttcta
     1561 acgtgttagt ccgcttatta aactctatca tggttttacc ccctttaaac catcaaattt
     1621 acctaaattc aaagcctcca tcttaatact aagataggga ttttttattt tgcggcattt
     1681 gttttgtatt tgtgtacgtg tgtatttatg tattattgta tacactctca gaattcgtgt
     1741 caatatattt tattaatatc tgggtgcata ttcttattca cattttgtgg gatctcatcg
     1801 attaaaattt cgaaaaagtg taaatgcccg atgcccctga aattttgctt atttaatgca
     1861 ttcctcttgt atcaacaaag ttttgtttgg ttactcaaaa agaaatatat gtaacatttt
     1921 acatataaac tgttttaaac aatttattta aaaaagaagg attttctcat ttcatatcga
     1981 aaataatata tatatgcacg tcttgacagg tttctattct cttattttta agctggaggg
     2041 aaagttatga agaaatccgt ttttggtttt aagcctttgt tgggattgtt tctattgata
     2101 gctattgctg taatgagctt catagcatcc ttgttggccc aaaggtggca agcagcaatt
     2161 tcagttattg tatccaatat cgtaatatta tctatagtat ccatagtttt ctatgctaaa
     2221 tttaagaaag tagcaaaaga gtttgagaaa gaagtcgacc tggtaaagca agaaggcttc
     2281 tcgaaaacta ttaatactga agagctgtat atgttgcaat ctacagctca tgttgttaat
     2341 ggattgttgt cggatataaa ggctttgatt aataactttc acaatctgtc aagatccata
     2401 attgatgcta caaaatctgt aagttcaaca gcccaacagg cttcaatagc aatggaggac
     2461 attgcaaaca ccatggatga tattgcaagc ggagcatctg accaggcaga acaggctcaa
     2521 cagggagttg aaatggttga caatctctcc gagcagataa actttgtgta tgaaagttat
     2581 aacaggatca ccgaagaaac caaaaaaata aatgacctga ataatatcgg tttggactct
     2641 gtaagcatac tacgggataa atccaaggaa aactatgaaa ctgcggaaaa gattttctcc
     2701 gttgttgaaa agctgacaaa tacaattaaa gatatagggc tgtttgtcga atctatcgaa
     2761 aacatagccg aacaaacaaa ccttttagcg ttaaatgctg ctatagaggc tgcaagagca
     2821 ggcgaagccg gaaaagggtt cgctgttgtt gctgaagaag ttagaaagct tgcagaccaa
     2881 agtagaaaat ccaccgaaga aataaatata ctcatgcaaa gcattcatga agaatctcaa
     2941 ttgtccatta agtccatgga gacaatgaaa agggtttcac aggaacaaaa cgttgcagtt
     3001 aacaagaccg acagtgcatt taacgatata gccaatgcca taacctatat tgtttccaag
     3061 ataaacgaag taaatcaagc cgtaaccaaa atgcaaacgg acaaaatcga ggttacatct
     3121 gcaattgaga acatttcttc tgtttctcaa gaaactgctg ccgcaagcca gcaggtagcc
     3181 gccaccacag aacatgagct taaggctata gaggcaatga atgaatcggc taaaaaactc
     3241 gagcagcttg tggaagagct tgacagcaag ctcaagaaaa atgctcattt catagccggt
     3301 gatgaaaacg atcaaccccc aacaatagag acgttttaga tatggaataa aatgggaatg
     3361 tgagagtaca tagagtagag aaataattgc taagaattat ttccctctcg ttgaaccgta
     3421 cg
//
LOCUS       DAOOJC010000262         3395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_21577_length_3395_cov_3.816467, whole genome shotgun sequence.
ACCESSION   DAOOJC010000262 DAOOJC010000000
VERSION     DAOOJC010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21577_length_3395_cov_3.816467"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..2099)
                     /locus_tag="PLA01_07455"
                     /pseudo
     CDS             complement(1..2099)
                     /locus_tag="PLA01_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518379.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
     gene            complement(2322..2537)
                     /gene="copZ"
                     /locus_tag="PLA01_07460"
     CDS             complement(2322..2537)
                     /gene="copZ"
                     /locus_tag="PLA01_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HPD01168.1"
                     /translation="MSKEIANLTVSGMSCSHCENSIKKSVGSLEGVDSVNVDLKSKKV
                     LVEYDTDKVNFETIIEIIEDQGYDVEK"
     gene            complement(2639..2761)
                     /locus_tag="PLA01_07465"
     CDS             complement(2639..2761)
                     /locus_tag="PLA01_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01169.1"
                     /translation="MVVAVIAGVVGDSLMDSFQNLQVYNSNGAEARLEQNKKIA"
     gene            complement(2830..3324)
                     /locus_tag="PLA01_07470"
     CDS             complement(2830..3324)
                     /locus_tag="PLA01_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPD01170.1"
                     /translation="MSEVLRGALLEQQEETQKDKFLTFTLGKEYYGIEIKYVTEIIGI
                     QPITEVPELPEHIRGIINLRGKIIPVMDVRLRFKKEFKEYNDRTCVIVIDAENILIGL
                     IVDSVSEVQSIPGEDIVVPPDINQSGNKYIKGIGKTGNDVKLILDCEKLLSEEELDGL
                     SNAI"
BASE COUNT          901 a          840 c          568 g         1086 t
ORIGIN      
        1 ttccgatgac gaacccggtt ccttgccgaa cacaagcaca ccgttatcat atacgggaat
       61 atactcagac tttacaacag tccctgccga aagcccttga taagaataat cgctttttgg
      121 atttaaggca gtaccctccg gtgctgaaat cctgaattgc acttccttct tgtacgccga
      181 ttgccctccg ggatatattt tggttcccga gaaatccact tcgacataat aaatattatc
      241 tttatattgc ttgacatcga aaaactttgc accctgatta tagcttgagc ttatcttaag
      301 gtcacctgcc gtgctgccgg aactcaaaaa ctctccaagg ttcatgaaat atttaaagga
      361 taagttttca catacccttg caggccatcc cgacttattg ttgaccaccg cttttatttc
      421 tataaagttg ttccggaagc atttattcct gcttcgacgt atatttcatc atccttgact
      481 tcctcgatac cgttaaattt ggggtcaggg cttcccccat aaagttggta catctttgcc
      541 aaaaggccga caaaacccgc attatagtca caagccactt cattgtttat ataatttcct
      601 atatcatccg tgtatgcatc cgaagcatca ggcccaccca caagggcacc ataaaggaca
      661 tgcctatgct cggaaggttc agactggctg tctgcccatg aaccatgagc agttctgtga
      721 tggggttttt tcggcggatt ttcaccgaag cctacgacaa agcttctgcc tgtgcttcct
      781 aaggcataat tagcctgact ctcggcaaaa tccatataaa cctttgcctt ctccttgtca
      841 caattctccc aatccgaata aacacaggca agaaatgcgg tagtagtggc atatctcaat
      901 gctccccatt gatcaagcca cgccagaccc tttggagtat atttaattct ttcaccgtta
      961 tatccggtgg tccaccaatc aaggtgcctt tcaatcgatt ctttatatat tcctttatcg
     1021 ttattgattt tcgctaaaag aagagtagca ccatagttta catcgtccca gcactgagcc
     1081 catttatatt tcggtgttgt tgtctgaggt tcgtagcccc aattgctgga atagctttgg
     1141 gccttatcaa gatatgaaga atccttggta gcgaggtaaa gccaagcacc tgcccaggaa
     1201 agctcatcat aaaacccgct ccaggaatca taaaaaccat tggccgcggt ataacctgta
     1261 tcacttttgg cggtatccgc aaactcaaac agctcttttg cgtgttttaa gcatttttcg
     1321 gaatatttct catccgcatc tctaaaaatt attgatgcca ccgccaatgc tgccgaggtt
     1381 ccggctgtaa ccgccgatcc cggcgaggat ttatcgactt taaatgaagg tctttccatt
     1441 gccattgctt ctgccgatcc ccaccatgcg tggtctgcgt gggcatcccc cacctgataa
     1501 taatacacat ccggctcagg gtggcatttt atgaaatagt cacatgccca tcttatgtta
     1561 ttgagcatgt ggttatactg tccgctttgt ttgaaggcat cctcatattc atatactgcc
     1621 cagcccagca tagcggccga ataagacatg ggcaaattaa acttcacatg atctcctgca
     1681 tcataccacc caccggtaag gtcgattcct gcatccttac cgtcatcaag gcccgaatcc
     1741 ccacgccaat ttaagcgaag gcttgaagga tcgagttttc ccgacttttg acactcgtaa
     1801 aagaaaatag ccttctgcaa tgcttcacca tagttataat caccggttgc aggaggctgc
     1861 ttcggaattt cctcctgaac caggtatccg tcgttcttat ccttgccatt attgtcagtc
     1921 tctgtagatt tgtcctttga ttttgaactg gagcttgttt ttggaccgga ctcatccgga
     1981 atatttgaag tttgctgtcc ccagggtctt gtaagaaaaa aagccgtacc taaaattatg
     2041 agaactataa atatagcaaa agctgttttc cttcgtttca aacagttaat caactgcata
     2101 tagtaatctt ctcccattga tattattgtc catctatcag tttattatat catattgtat
     2161 cgggagaatt tacataaaaa gtgatgataa tttttccgtc ccgaatcata ggaataacga
     2221 gcatccgaca atatagttac cgggatttta tattttgccg gagtcttcat acagttggag
     2281 catgcttttt aaatcccttt ccgcggcttt agtctatgat cttacttttc tacatcataa
     2341 ccctggtcct caatgatttc tataatggtt tcaaaattaa ccttgtcagt atcatattcc
     2401 actaaaacct ttttgctctt gagatctaca ttgacactgt caactccctc cagagacccg
     2461 acagattttt taatactgtt ttcacagtga ctgcaggaca ttccgctaac cgttaaattt
     2521 gctatttcct tagacatacg tcaaacctcc cgtttgattt atttctatcg ctgcattcaa
     2581 acctaatagc cacaagtatg cctacaacta tcacaattat catcgttgtt gaagcatttt
     2641 atgctatttt cttattttgc tccagtctgg cttccgcacc attagaattg tatacttgta
     2701 aattttgaaa tgaatccatc aacgagtctc caaccactcc tgcaatcact gcaacaacca
     2761 aaatccgata accagttttg ctgatatttt taaattataa aaccaacgca ttatgtaatc
     2821 ctacttgatt tatattgcat tacttaagcc atccagttct tcttccgaaa gcaacttctc
     2881 acaatccagt atcagcttaa catcatttcc ggttttgcca atacctttaa tatacttatt
     2941 tccgctctgg tttatatccg gtggtacaac aatatcttca cccggaatgg actgaacttc
     3001 tgaaacactg tctacaatca gcccaataag tatattttca gcatcaataa caattacaca
     3061 agtcctatca ttatactctt taaattcctt tttaaatctt agtctcacat ccattacagg
     3121 gattatcttg cctcgcaaat tgattattcc tcgaatatgc tccggcaatt cgggtacttc
     3181 ggtaataggt tgaatcccta taatttctgt cacatacttg atctcaattc cgtagtactc
     3241 cttccctaga gtaaatgtta gaaacttgtc cttctgtgtt tcctcttgct gttccagcaa
     3301 tgcacctctt aacacttcag acattttttc acctccttgt acgattagtc ctcttatttt
     3361 atcagtggct tttgccactg ttcgaccctg ttaac
//
LOCUS       DAOOJC010000263         3375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_21745_length_3375_cov_3.973193, whole genome shotgun sequence.
ACCESSION   DAOOJC010000263 DAOOJC010000000
VERSION     DAOOJC010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21745_length_3375_cov_3.973193"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PLA01_07475"
     CDS             <1..319
                     /locus_tag="PLA01_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPD01171.1"
                     /translation="NMFAPEDYLTTADAITIIIRALGLEGLAPSTGAVTVFRDNEDIP
                     EYARGSVYVAQRIGLVLGDEKGYLKPREYLTKARAAVIINKLINYMRDDLKKDYRERI
                     VNY"
     gene            354..1862
                     /locus_tag="PLA01_07480"
     CDS             354..1862
                     /locus_tag="PLA01_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPD01172.1"
                     /translation="MTRKSIKMTLVYLIVCSLLMSGYVFVQAAEEQVGGSKDSDYIEN
                     VMDMVLDRYKGEVDRQKLLEGALKGLFSTMDPYTTFYTKEEAESFMVSINGTFVGIGI
                     AFSEVNGKFVIEYVYSSSPAEEAGLRAGDIIIEVDRRSMVGRSLEEISNFLLGEEGTN
                     VTVGIQRGGNSEIIRYELTRREIKIEPVSYSIDGKIGYIKLESFNSNACEAVDKALDH
                     MNKNGITKIILDLRDNPGGEVNQAVLIARRFVPEGLITKLDFKSESMKDESYYSYLKE
                     PKYKLVVLVNRSSASASEILAGAIQDTSAGTLVGTKTFGKGKIQNLFPILTPEASDKY
                     GEELGGSIVDAQDLFIKSGKSPKEDEIIGWIKITTGEYYTPNGRMIDGVGLEPDVYVE
                     NPVDVSMGIALERIEKLKKVIKPSLNDENEEVYNAESILMFAGYDVDKPDNIMDEKTV
                     QAVAKFQKDCGMYSYGVLDFATQQALNEKLDELLSNKDMQYLKAVELLSGEQ"
     gene            2062..2976
                     /locus_tag="PLA01_07485"
     CDS             2062..2976
                     /locus_tag="PLA01_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HPD01173.1"
                     /translation="MKFKNVIKNNKGTTMVIYVAAISVLIGCASISVDIGHIMLEKAE
                     ITKAVDAAALAGAQEFANNEGNPYRVACDYLEKNGVSPDEADITILEDGISILANREV
                     NYFFARILGFEKGEVEAAAVARALPVISVDGGVRPFAIEDQPLLYGERYVLKEGGGDG
                     SNGNYGCLALGGNGASNYRYNILYGYDGELEVGDYVNTEPGNMSNPTKSSIDTLIRQC
                     NHAPECTIHSYDPDCPKIVTVVIVEDMDVNGRGPVRICGFASFLIEEVAGQGNQCQLA
                     GYFIQNVAQGELSEGQTDYGLKGVKLIR"
BASE COUNT         1099 a          490 c          851 g          935 t
ORIGIN      
        1 taacatgttt gcaccggagg attatctaac cacagcggat gcaattacaa tcattatacg
       61 ggctttggga ttggagggac ttgcaccttc aacaggagcg gttacggtct tcagagataa
      121 tgaggatatt cccgaatatg caaggggttc ggtttacgta gcccagagga ttggtcttgt
      181 gcttggggat gaaaagggat atctcaaacc gagagaatac ctgactaaag cgagggctgc
      241 agtaattatc aataaactga taaattatat gagggatgat ttaaagaagg attaccggga
      301 aaggatagtg aattattagc aatgaaattt tataatagaa aaggggaatt aacgtgacga
      361 gaaaatcaat aaaaatgaca ctggtttatt taatcgtctg ctccctctta atgtccggct
      421 atgtttttgt acaagcggca gaggagcagg ttggaggttc aaaggattcg gattatattg
      481 aaaacgttat ggacatggtg ctcgacaggt acaaaggaga agtagaccgt caaaagctat
      541 tggaaggtgc cttgaaagga ttgttcagta ctatggatcc ctataccacc ttttatacga
      601 aggaagaggc cgagagcttt atggtaagca ttaacggtac ttttgtcggt ataggaattg
      661 cgttttcaga ggttaatgga aaattcgtta tagagtatgt atacagttct tcaccggcag
      721 aggaagcagg tcttagagca ggcgatataa taattgaggt ggacagaaga agcatggtgg
      781 gacgttccct tgaggaaatc agcaattttc tgctagggga agaaggtaca aatgttacgg
      841 taggtataca gagaggcggc aattcggaaa taataaggta tgagcttaca aggcgtgaga
      901 tcaagattga accggttagt tacagtatag atggcaaaat agggtatatt aagctggagt
      961 cttttaattc aaatgcatgc gaagctgtgg ataaagctct tgaccatatg aacaaaaacg
     1021 gtataacaaa gatcatactt gatttaaggg ataatccggg cggagaggtg aaccaggctg
     1081 ttctaattgc ccgaaggttt gtgcctgaag gtttgattac gaagctcgac tttaaatcgg
     1141 aatccatgaa ggacgaaagt tattattcat atttaaagga gcccaagtat aagcttgtgg
     1201 tgctggtgaa cagaagcagt gcaagtgctt cggagattct ggctggtgca atacaggata
     1261 ccagtgcggg gacattggtg ggtacaaaaa cttttggcaa aggaaagatt caaaaccttt
     1321 tccccatact tacacctgaa gcttccgata agtacgggga ggaactcggt ggtagtattg
     1381 ttgatgctca ggatttgttt ataaaaagcg gtaaaagtcc caaggaggat gaaattatag
     1441 gttggattaa gataacaacg ggtgagtatt atacacctaa cggaagaatg attgacggtg
     1501 tgggccttga gccggacgtg tatgtggaga accccgtgga tgtcagcatg ggtatagcct
     1561 tagagaggat tgaaaaactc aaaaaagtaa taaagccaag cttgaacgat gagaacgagg
     1621 aagtatataa tgccgaaagc atccttatgt ttgcaggcta tgatgtggat aaaccggata
     1681 atatcatgga tgaaaaaacc gttcaggcag tagcaaaatt ccaaaaggat tgtggaatgt
     1741 attcttacgg tgtactggat tttgcaacac aacaggcact gaatgaaaag cttgacgaat
     1801 tactttcaaa taaagacatg caatatctga aggctgtaga attactatcc ggtgagcaat
     1861 gagaaccttt tgaaacaaat taatataggt acaaagatac tatgaaaatg gttatgacgc
     1921 tctatataaa gagaaaccgt ttttcagtat aataaaacta taagcgagag tgcttatagc
     1981 cgataatgga ccctgggata aacagaatgt tttgcacata agtttgtctt tagggtccaa
     2041 ctattttagg ggtgatgagt tatgaaattt aaaaacgtta taaaaaacaa taaaggtaca
     2101 actatggtga tatatgtggc tgctatttcg gttcttattg gctgtgcatc catatcggtg
     2161 gatataggtc atatcatgct ggaaaaggcg gagattacaa aagccgttga tgctgcagca
     2221 ttggccggag ctcaggaatt tgcaaacaat gaaggaaatc cttatcgtgt tgcctgtgat
     2281 tatctggaga agaatggtgt cagtcctgat gaggcggata taactatttt ggaagacggt
     2341 atcagtatat tggccaacag ggaagtaaat tacttttttg caaggatact tggatttgaa
     2401 aaaggagagg tggaggcagc agctgttgcc agagcacttc ctgtgataag tgttgacgga
     2461 ggggtaagac cttttgcaat agaagaccag cctttgcttt atggggaaag atatgtcctt
     2521 aaagaaggcg gaggagacgg aagcaatgga aattatgggt gtttggccct tgggggaaac
     2581 ggggcatcaa attacagata caatatttta tacggatatg atggagagct ggaggtcggg
     2641 gattatgtaa ataccgaacc gggaaacatg agcaatccta cgaaatcgtc gatagacaca
     2701 ctaatcaggc agtgcaacca tgccccagaa tgcactattc acagttatga tcctgactgc
     2761 cctaaaatag tgacggttgt tattgttgaa gacatggatg taaacggaag gggcccggtt
     2821 agaatatgcg gatttgcatc tttcttaatt gaagaggtgg caggacaggg aaatcagtgc
     2881 caactggcgg gttattttat tcagaatgtt gcacagggcg aattgtccga gggtcagacc
     2941 gattatggat taaagggcgt taagctcata aggtaaatgg gacaaacctt acttatggag
     3001 actgtattgg tatactgcat aatttcatct ttgaaagtta ataattatag aaagcctgta
     3061 gatcaatgaa attaaaggat ttacaggctt taatgtttta tgactttttt aagcattttt
     3121 tgcacggaga ataataccaa tagagctata gaattctaag aaaaatgtaa aattataaat
     3181 tgactattga aatatttgtc tgtttagtat aaaatttaac atgataatat ctacaatttg
     3241 taattcaagg gttaataaac gatttatgta tattatttat agaatgatag tataactttt
     3301 cggtaatggc atttgaggta gttatttgtt gtgggcaata cttacttgaa tgtttctttt
     3361 tcagcttaat aaatt
//
LOCUS       DAOOJC010000264         3374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_21751_length_3374_cov_3.430551, whole genome shotgun sequence.
ACCESSION   DAOOJC010000264 DAOOJC010000000
VERSION     DAOOJC010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21751_length_3374_cov_3.430551"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLA01_07490"
     CDS             <1..374
                     /locus_tag="PLA01_07490"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPD01174.1"
                     /translation="HRTIQKIKQLGKKAAVALNPATPLSSIEWVLDDVDMVLIMSVNP
                     GFGGQKYIEGITRKTESLKSLIKTRGCNIDIEVDGGIDTSNIYDVTKAGANVIVSGST
                     IFSAKDTVKIIKELKENAYRE"
     gene            389..1024
                     /locus_tag="PLA01_07495"
     CDS             389..1024
                     /locus_tag="PLA01_07495"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HPD01175.1"
                     /translation="MYAVVVSSGSIIDYSYYDKLFHEAGFIVCADGGAAHLKKMGIVP
                     DVLVGDFDSIDAGHLQYYREKEVEIFKYPEEKNMTDTELAVNVAIDRGYRDIVIIGGT
                     GGRLDHTLSNILLLKKMLDRGVTGKVVNEHNEIFLTAGSAEVMAKEEYNLTLLPVTER
                     VEGITTKGLYYPLMGETIEMGSSRGVSNKFVAGKAQISITSGILAVIKSRD"
     gene            complement(1291..3330)
                     /locus_tag="PLA01_07500"
     CDS             complement(1291..3330)
                     /locus_tag="PLA01_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPD01176.1"
                     /translation="MKGGWFDAGDYVKFNLPMAYTVAMLAWTVYEYKDALEKSGQLTY
                     VMDQIKWVSDYLIRCHPEKNVYYYQVGNGDYDHRWWVPAECIDLQAARPSYNVDMSNP
                     GSTVTAETAAALAATAVVFKDSDPSYAALCLQHAKDLFDFAEATKSDKGYTAALNFYT
                     SHSGWYDEFSWAGAWIYLAGGGQTYLDKAEQYVSEWGLESQTPYIAYSWGHCWDDVHY
                     GAALLLAKITNDTFYKETIERHFDHWTTGFNGNKIRYTPKGLAHLTDWGALRHSTTTA
                     FLACVYSDWEGCSPEKVKTYMDFAKSQADYALGSAGRSYVCGFGTNPPKSPHHRTAHS
                     SWCDTMKDPKEHRHILYGALVGGPDSSDGYSDDVANYVNNEVACDYNAGFVGLLTRMY
                     DLYGGNPIPNFMAIEEITNEEIYVGAKASLGSSLAIKSFLYNKSGWPARMCDKLSFRY
                     YMDLSEYVSAGFDPADISTSIVYTAANAATISKPQVYDASKNIYYIEIDLTGTKIYPG
                     SNMDHQKEVQFNIQPPGGAPWNNANDFSYQGIMDDEEIVPNIPVYDNGVLIFGEEPDG
                     TVPGTPPPPPNTSEPTSTKKNTPTPTPTKKDIPTPTAPTSSPADVKRGDINLDDVVDS
                     TDLTLLKRYVMRKLKEFPSDDTAKSLIAADANNDGSIDSTDVTIVKRVILRKLVL"
BASE COUNT          933 a          675 c          765 g         1001 t
ORIGIN      
        1 tgcaccgtac tattcagaaa ataaagcagc tggggaaaaa agctgccgtg gcattgaatc
       61 ctgccacacc cttaagttcc attgagtggg tgctggacga tgtggatatg gttttaatta
      121 tgtctgtaaa tccggggttc ggaggacaaa agtatataga gggtattacc cgtaaaactg
      181 agtcgcttaa gagtttaata aaaacccggg gatgtaatat tgatattgag gtagatggag
      241 gaatagacac cagcaatatt tatgatgtta ccaaagccgg ggcaaatgtt attgtatcgg
      301 gatctacaat attttccgca aaggatactg ttaagataat taaggaactc aaagaaaatg
      361 cttacaggga ataggaccgg tgaaaattat gtatgctgtt gttgtttcca gtgggagtat
      421 tattgattat tcatactacg ataaactgtt ccatgaagca gggtttattg tttgtgccga
      481 cggaggtgcc gcacatctta agaagatggg aattgtgccg gacgtccttg tgggggattt
      541 tgattctatt gatgccgggc atcttcaata ctatcgcgaa aaagaagtag aaatatttaa
      601 atacccggaa gagaaaaaca tgaccgacac ggagcttgct gtaaatgtgg caattgacag
      661 agggtacagg gatattgtca ttattggggg aacgggcggc agactcgacc atactctgtc
      721 caatattctt ctcttaaaaa agatgttaga ccgtggtgtt acgggaaaag ttgtgaatga
      781 gcataatgag atttttctta cagccggtag tgcagaggtt atggccaagg aggaatacaa
      841 cttaaccctg ctgcctgtga ctgaaagggt tgagggaatt accacaaaag gtctgtatta
      901 tcccttaatg ggtgaaacca ttgaaatggg atcgagcagg ggtgtgagta acaagttcgt
      961 tgccggaaag gcacagattt ctattacttc gggaatactg gctgttataa aatccagaga
     1021 ttagcagcag ttcaagatca ttgaggtacc atcttattct aaccgtttca cttcgtgaaa
     1081 tcagtttaaa taaaagctct tggggcatcg atataccatc ttttttagtc atttcacttc
     1141 gtgaaatccg cttaaataaa agttccggga gcctcgagcc gccatcgttt ttttggccat
     1201 ttcgcttcat gaaatttaca tttaaataag caccagggac aaaataccct ggtgcttttt
     1261 aaaaggaaag aatggagtaa atatcatcga ttacaatact aacttacgta aaataacacg
     1321 cttaacaata gttacatctg tggagtctat tgatccatca ttatttgcat ctgcggcaat
     1381 aagactttta gcggtgtcat cacttggaaa ttctttaagt ttcctcataa catacctctt
     1441 taataaagtc aggtctgttg agtctaccac gtcgtccaga ttaatgtctc cgcgttttac
     1501 atccgccggt gaactggtgg gtgcagtggg ggttggaata tccttctttg taggagtagg
     1561 tgtcggagta tttttcttag tagaagttgg ttcagaagtg ttgggtggtg ggggaggtgt
     1621 accgggaaca gtaccgtccg gttcttctcc gaatatcaaa actccattat catatactgg
     1681 tatattcgga acaatttctt cgtcatccat aattccctga tatgaaaagt cgttggcatt
     1741 attccaagga gcaccgccag gtggctgtat attgaactgg acttctttct gatggtccat
     1801 gttgcttccg ggatatattt ttgtgcctgt caaatctatt tctatatagt agatgttttt
     1861 cgaagcatcg taaacttgag gctttgaaat agtagctgca ttggcagcag tataaactat
     1921 gctggtgctt atatcagccg ggtcaaatcc tgcagacaca tattccgaca gatccatata
     1981 gtatctgaag gaaagcttgt cacacatcct tgccggccat cccgatttgt tataaaggaa
     2041 ggatttaata gccaaactgc ttccaaggct tgcttttgct ccaacataga tttcttcgtt
     2101 tgttatttct tctatagcca tgaaattagg tatggggtta cctccataga ggtcatacat
     2161 ccttgtgaga agtccgacaa aaccggcgtt ataatcgcag gcaacctcat tgtttacata
     2221 gtttgcaaca tcatcagaat acccgtcact ggaatccggt ccaccaacca atgcaccata
     2281 aagaatatgt ctgtgttctt taggatcctt cattgtgtca caccatgaac tgtgagcggt
     2341 tctgtgatga ggactctttg ggggattggt accgaagccg cacacatagc tcctgccggc
     2401 acttcccaat gcgtaatctg cctggctttt cgcaaaatcc atgtaggttt ttaccttctc
     2461 aggggaacat ccttcccaat cggaataaac acaggctaag aatgctgtgg ttgtagaatg
     2521 ccttaatgca ccccagtctg tcaggtgggc taaacccttg ggagtatatc ggattttatt
     2581 gccgttaaaa ccggttgtcc aatgatcaaa atgtctttca atggtttctt tatagaaagt
     2641 gtcatttgta atttttgcta ataacaaagc tgcaccgtaa tggacatcat cccagcagtg
     2701 tccccatgaa taggcaatgt aaggggtttg ggattcaaga ccccattcgc taacatattg
     2761 ttctgccttg tcaagatagg tttgaccgcc gccggcaagg tagatccagg cacctgccca
     2821 ggagaactca tcataccaac cgctgtggga tgtataaaag ttcagtgcag cagtataccc
     2881 tttatcactc ttggttgctt ctgcaaaatc gaataggtct ttagcatgct ggagacacag
     2941 agcggcatat gacgggtcag aatccttgaa tacaactgct gtagctgcaa gtgctgcagc
     3001 tgtttctgca gtaacggtgg agccggggtt tgacatgtcg acattataag acggtcttgc
     3061 ggcctgaagg tctatacatt cggcaggaac ccaccaccta tggtcataat caccgtttcc
     3121 cacctgatag tagtatacgt tcttttcagg gtggcatctg ataaggtaat cggataccca
     3181 tttgatttga tccatcacat aagtcaattg accgcttttt tccagagcat ccttgtactc
     3241 ataaactgtc caggcaagca tggctactgt ataagccatg ggaaggttga atttaacgta
     3301 gtctccggca tcaaaccaac ctcccttaat atagtcttat tttcttcgtt tatctttact
     3361 tttcacagta tttc
//
LOCUS       DAOOJC010000265         3362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_21853_length_3362_cov_3.009374, whole genome shotgun sequence.
ACCESSION   DAOOJC010000265 DAOOJC010000000
VERSION     DAOOJC010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21853_length_3362_cov_3.009374"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLA01_07505"
     CDS             <1..195
                     /locus_tag="PLA01_07505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPD01177.1"
                     /translation="IEHISKQLNVRLIDIRKAFLDAKNLMELLCRDGIHPNEKGHEII
                     ASCLIEYISDYKREVLQGAV"
     gene            240..1322
                     /locus_tag="PLA01_07510"
     CDS             240..1322
                     /locus_tag="PLA01_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01178.1"
                     /translation="MKKVCLLIICLFILSFILTGCDNQPWINMVDTLMQGDYRKANKQ
                     YEKIEKNYVYAMNTSEYVYQYIENIVTSFKQDKTSYETAEKKLNLFSLLEMPLYNLED
                     AKSEVERIQASRTAYNQARELYLAELYYDAHNKFKLVEIDDSRNYDKAQEYLLSIVYV
                     LLYREEYYKAADVILSVDINSNTVPKLAEYSVDLAIRLLYYKDDMDRAYELNDKLIKL
                     LIRMEPGVKDAIKEWSRKLESIYSRPEYAAVCISFLSDDMEDMFYKLEQTDLRLETQK
                     TDYAFIILKSIPVRQDTIGYIAPRMTEILYQYISQGKPDYAQSVVDIIFSEIDDDRMV
                     KSLVTYIQDNIKNKEYLNKIDMTVGR"
     gene            1380..1467
                     /locus_tag="PLA01_07515"
     tRNA            1380..1467
                     /locus_tag="PLA01_07515"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1414..1416,aa:Leu,seq:taa)
     gene            1474..1550
                     /locus_tag="PLA01_07520"
     tRNA            1474..1550
                     /locus_tag="PLA01_07520"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1508..1510,aa:Met,seq:cat)
     gene            1668..2609
                     /locus_tag="PLA01_07525"
     CDS             1668..2609
                     /locus_tag="PLA01_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01179.1"
                     /translation="MEIREIEKIINEKISPYSLNEEGISIVSKLVNNFSFELLKECID
                     TGVNAYFRFDKEGNLTEESVDTFMKKLGGIAYNRLKTPLDKQIFSIKNRCWHKFPDWN
                     TDKANDILIRYISALKSKGLTEELIINDLQTDVSKMCSQSSDWSDWSSGMEYWIDNIK
                     DWPDEDSVAIKHNESIITNDVIEGFLHNSPNMQSLCKQINASYENNLYDCTAVIMRRL
                     LENLLILTYENKELEDDIMDESGRHYLPLSDIIKNAEKNPKLHLSSNVRKDMSLFKDL
                     GNDSAHKIRYNSTKQDIEPHIIKYRTIVRELIKKAGL"
     gene            complement(2787..>3362)
                     /locus_tag="PLA01_07530"
     CDS             complement(2787..>3362)
                     /locus_tag="PLA01_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD01180.1"
                     /translation="PKEREMVAYHEVGHALVSALQKNSQPVQKITIVPRTMGSLGYTM
                     SMPEEERYLMTESEILAQITTLLGGRAAENLIFGEVSTGASNDIERATKLARGMITQY
                     GMSDKFGPMGLESTQNQYLDGRNVAHHSEQTGALIDKEVSSILVQCQQNAFNLLQDNR
                     DALERISKYLLEKENITGKEFMQLLEETRSK"
BASE COUNT         1125 a          564 c          667 g         1006 t
ORIGIN      
        1 atagaacata tttccaagca acttaatgtc cggcttatag atataaggaa ggcattcctg
       61 gatgcaaaaa atctgatgga actgttatgc agagacggca ttcatcccaa tgaaaaaggg
      121 catgaaatca tagcaagctg tctcatagaa tatatatccg attacaaaag agaagtttta
      181 caaggagctg tttaacgcat tatattgaat gaaaaggcaa tatgggatat acttttttta
      241 tgaaaaaagt atgcctgctt ataatttgcc tttttatatt atcctttata ctgacaggtt
      301 gtgacaacca gccgtggatt aacatggttg acactttaat gcagggtgat tacagaaaag
      361 ccaacaaaca gtatgagaaa attgaaaaga attatgtata tgcgatgaat acatcggagt
      421 atgtctatca atatattgaa aacatagtta catcattcaa acaggacaag acctcatacg
      481 aaaccgcgga aaagaaactg aatctttttt cgctgctgga aatgccgctg tataatctgg
      541 aggatgcaaa aagcgaagtt gaaagaatac aggcatcaag aacagcctat aatcaagcaa
      601 gggagttgta tcttgccgaa ttgtattacg atgcccataa taagttcaag cttgtggaaa
      661 tagatgattc cagaaactat gataaagctc aggaatatct cttaagcatt gtatatgtgt
      721 tgctttatag agaagaatac tataaggctg ccgatgtcat cttatcggtg gatataaaca
      781 gcaatacggt acccaaactt gcggagtatt cagtagatct ggcgataaga cttctgtatt
      841 acaaggatga tatggataga gcttatgaat taaatgataa attgataaaa cttcttatac
      901 gcatggagcc cggcgttaaa gacgcaatta aggaatggtc aagaaaactg gaatcaattt
      961 attcccgtcc ggaatatgcg gcggtgtgta tttctttcct ttcggacgat atggaagaca
     1021 tgttttacaa gttggagcag accgatttac gcctggagac acaaaaaacc gattatgcat
     1081 ttattatcct taaatccatc cctgttcgtc aggatactat agggtatata gcccccagaa
     1141 tgaccgaaat tctgtatcaa tatatctcac agggcaaacc tgactatgct cagtcggtag
     1201 tagatataat cttttcggag attgatgatg accggatggt taagtctttg gtgacctata
     1261 tacaggataa tattaagaat aaagagtatc tcaataagat agatatgact gtaggaagat
     1321 aggtttctgt tgacaataga caaagtggtg tatataatag gaaaagtctc taaagccatg
     1381 ccgaagtggc ggaataggca gacgcacagg acttaaaatc ctgcggtcga atagactgta
     1441 ccggttcgac cccggtcttc ggtaccagta tgccgcggag tagagcagtc cggaagctcg
     1501 tcgggctcat aacccggagg tcaaaggttc aaatcctttc tccgcaacca ataaagaaaa
     1561 cagccgtgca tattgatggc tgtttttttt atcttaaaat ataatcaaaa caatagttgt
     1621 agagttattg acatatttag tataaaatta tattgcgagg tgaaataatg gaaataaggg
     1681 aaattgaaaa aataataaat gaaaagatat ctccatattc tctcaatgaa gaaggcatat
     1741 caattgtttc aaaattagtg aacaattttt cctttgaatt gttaaaagaa tgtattgata
     1801 ccggagttaa tgcatatttt aggtttgaca aagaggggaa tttaactgag gaatcagttg
     1861 atacctttat gaaaaaactt ggtggaattg cttacaacag gcttaaaact cctcttgata
     1921 aacaaatatt ttccataaaa aataggtgtt ggcataaatt tcctgattgg aatactgata
     1981 aagcgaacga tatcttgata agatacatta gtgcacttaa aagtaaaggt cttacagagg
     2041 aattgattat taatgattta caaactgatg tgagtaaaat gtgttcgcaa tcgagtgact
     2101 ggtctgattg gtcctctggt atggaatatt ggattgataa tataaaggat tggccagatg
     2161 aagacagtgt tgccattaag cataatgaat caataataac taatgatgtt attgaaggat
     2221 ttttacataa ttcgccaaat atgcaatctt tatgtaagca aattaacgca agttatgaaa
     2281 acaatttata tgactgcaca gcagttatca tgcggcgttt gttagaaaat cttcttattc
     2341 ttacgtatga aaataaggaa cttgaagatg atattatgga tgagagtggg agacactatt
     2401 tgcccctaag tgatatcata aaaaatgctg agaaaaatcc taaacttcat ttatcctcga
     2461 atgtgcgaaa agatatgagt ttgtttaaag atcttggtaa tgactctgct cataagatac
     2521 gatataactc aactaagcaa gatattgaac cgcatattat aaaatatcgt acaattgtta
     2581 gagaattgat taagaaagcc ggattataat aaatacacaa aaccatcaaa atataacaat
     2641 aaaagagcaa attattttaa ccgctttctt tatgcaaaga tactttattt cctttaaaac
     2701 aaaagggatg aatttagcag taaaaatatt taataaaaaa gctccaagtt tgactcagag
     2761 cttttcctgt attttttgtt tatcactcat tttgaacgtg tttcttcaag cagttgcata
     2821 aactccttcc ctgttatgtt ttctttttct aagagatatt ttgatattct ttccaaagca
     2881 tctcggttat cctgcagtaa attgaaagca ttctgctgac attgaacaag tatactgctg
     2941 acctctttat caattaatgc accggtctgt tccgagtgat gagctacatt gcggccgtcc
     3001 agatactgat tttgtgtgct ttccagtccc ataggtccga acttgtcgct cataccgtat
     3061 tgggtaatca taccccttgc cagtttggtt gcccgttcga tgtcattgga tgcaccggta
     3121 cttacttctc cgaatataag attctcggca gctcgtcctc ccagcagggt ggtaatctga
     3181 gcaagaatct ccgattcggt cataagatag cgctcttcct ccggcatact cattgtatag
     3241 cccagacttc ccatagtacg gggaacgata gtaatcttct gaacaggttg gctgtttttc
     3301 tgaagggcgc tgaccaaagc atgtcctact tcgtgataag ctaccatctc acgttccttg
     3361 gg
//
LOCUS       DAOOJC010000266         3341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_22032_length_3341_cov_4.168594, whole genome shotgun sequence.
ACCESSION   DAOOJC010000266 DAOOJC010000000
VERSION     DAOOJC010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22032_length_3341_cov_4.168594"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2730)
                     /locus_tag="PLA01_07535"
     CDS             complement(<1..2730)
                     /locus_tag="PLA01_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPD01181.1"
                     /translation="MARLEDLKAGTVVKGILPNGNVTVIDAKWIGNVAIELTYKDDSG
                     NVNSELIYRDKEPMLEIVQAGLPWSFTADGEMLRLASEAYRIKLAHLFDPYLAVHTSM
                     VEPLPHQITAVYGEMLTRQPLRYLLADDPGAGKTIMTGLLIKELLIRGDLKRCLIVTP
                     GNLSEQWQDELDRRFGLPFEIITNDRIESARTGNVFMEIPLCIARLDKLARNEDIQAK
                     LRMTDWDLIVCDEAHKMSASFFGGEVKYTKRYRLGQLLSSITRHFLLLTATPHNGKEE
                     EFQLFMALIDGDRFEGKFREGYHSTDVSDIMRRMVKEELLKFDGTPLFPERIAYTVNY
                     KLSDEEALLYKEVTEYVREEFNRADNLENDGRKGTVGFALTILQRRLASSPEAIYQSL
                     RRRRERLEKRLNEEKLKKRGMDAANRIFDCPVLSSEDIEELEDAPGNEVEDIEEVVVD
                     QATAARTIAELEVEIEKLRALEAMAYKVRNSGRDRKWDELSKLLQDDQRMFDSEKNRE
                     KLIIFTEHKDTLNYLADKIRALLGKQEAVVTIQGGMLREERRKAQEKFTQDKEVRIMV
                     ATDAAGEGINLQRAHLMINYDLPWNPNRLEQRFGRIHRIGQTEVCHCWNLVADETREG
                     EVFQRLLKKLEEERRALGGRVFDILGKITFDNKPLRELLIEAIRYGDRPEVKARLHKV
                     LDNSLDREQLKKLFEERALAKNSMDARKVMEIREEMERIEARRLQPHFIESFFISAFR
                     YLGGTIREREPRRYEITHVPAVIRNRDRVIGLGEPVLSRYERVCFEKELINVEGKPLA
                     SFICPGHPLLESVIDLIRERNADVLKRGSILVDETGSSNELRVLFYIENSIQDACTDV
                     NGKRRIVSKQVHFVEIDCNGNKRNAGYAPYLDYRSLKEEEKEKAGDILEQASWL"
     gene            complement(2760..2939)
                     /locus_tag="PLA01_07540"
     CDS             complement(2760..2939)
                     /locus_tag="PLA01_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPD01182.1"
                     /translation="MEYMTAKEAAQKWNITVRRVQVLCAHGRIQGATRLGNIWAIPKD
                     AKKPNDARSKTDRRK"
BASE COUNT          861 a          855 c          540 g         1085 t
ORIGIN      
        1 caaccaacta gcctgttcaa gtatgtcgcc ggctttttct ttttcttcct ctttaagact
       61 tctatagtcg agatatggag catagccggc atttctttta ttgccattgc agtctatctc
      121 tacaaaatga acctgctttg acactatcct tctcttaccg tttacatctg tacaggcatc
      181 ctgtatggag ttttcaatat aaaacaacac tctcaactca ttactgctac ctgtctcatc
      241 tacaagtata gacccccgct tcaacacatc cgcatttctt tctcttatta aatctattac
      301 tgactcaagc aatggatggc cgggacaaat aaaagacgca agaggtttac cctctacatt
      361 gataagctct ttttcaaaac atactctctc atacctagat aacaccggct ctcctagtcc
      421 tataactcta tcacggtttc gaatcacagc aggcacatga gtaatttcat atcttctggg
      481 ttctctttcc ctgatagtgc cacccagata cctaaatgca ctaataaaga acgattcgat
      541 aaaatgtggc tgcagtcttc ttgcttcaat cctctccatt tcttcccgga tttccatgac
      601 ttttcttgcg tccatactgt ttttcgcaag agctctttct tcaaaaagct ttttcaattg
      661 ctccctgtca agagaattgt ccaacacttt atgaagacgt gcttttactt ccggtctgtc
      721 cccatagcgg atagcttcaa taagaagctc tcttagcggc ttgttatcaa atgttatctt
      781 gccaagaata tcaaagaccc gtccgcctag ggctcttcgt tcctcttcca acttttttag
      841 aaggcgctgg aatacttctc cttcacgggt ctcatccgca accaaattcc aacaatggca
      901 gacttctgtc tgcccaattc tatgaattcg cccaaatctt tgttccagtc tgttaggatt
      961 ccaaggaaga tcataattaa tcatcagatg tgcacgctga agatttatac cctctccggc
     1021 ggcatcggta gccaccatga tgcgtacttc tttgtcctgc gtaaattttt cctgagcttt
     1081 cctccgttct tccctcaaca tcccgccctg tatggtcact accgcttcct gtttgcctaa
     1141 cagtgcccgg attttatccg caagatagtt aagtgtatcc ttatgctcgg tgaatatgat
     1201 aagcttttcc cggttttttt ctgagtcaaa cattctctgg tcatcctgta agagtttaga
     1261 cagttcatcc catttcctgt ccctgccact atttcttact ttataagcca tcgcctcaag
     1321 tgcccttaat ttctcaattt ctacctcaag ttccgcaatg gtacgcgcag ctgtagcctg
     1381 atcgacaact acttcttcta tatcttcaac ttcattgcca ggtgcgtctt caagttcctc
     1441 aatatcttca cttgacagca caggacaatc aaaaatccga tttgcggcat ccattcccct
     1501 ttttttaagt ttttcctcgt taagtctttt ttccaatctc tcacgccttc ttctaagaga
     1561 ttggtaaata gcttcaggag aagatgccag tctcctttga aggatggtta atgcgaatcc
     1621 gacagtacct tttctgccgt cgttttcgag attatccgcc ctattaaact cttcacgcac
     1681 atattccgta acttctttat aaagcaatgc ttcctcatct gagagcttgt aattaactgt
     1741 atatgcaatc ctttctggga acagaggtgt accatcaaat tttaacagtt cttccttaac
     1801 catcctacgc ataatgtccg acacatctgt tgaatgatag ccctcccgga actttccttc
     1861 aaacctgtca ccatctataa gggccataaa aagctggaat tcttcttctt taccgttatg
     1921 cggcgtcgct gtaagcaaga ggaaatgtct tgtgatgctt gacaaaagct gtccaagcct
     1981 atacctcttg gtgtacttca cttctccgcc aaaaaacgaa gcagacattt tatgggcctc
     2041 atcgcataca atcaaatccc aatcagtcat tctaagcttc gcctggatat cctcatttcg
     2101 tgcaagttta tcaagccttg ctatacataa gggtatttcc ataaaaacat ttcccgttct
     2161 tgcagattca atcctgtcat ttgttataat ttcaaaaggc agcccgaaac gcctgtccaa
     2221 ctcatcctgc cattgttcgg aaaggttgcc gggagtcaca attaaacacc gtttaaggtc
     2281 gcctcttata agcagttctt ttattaataa acctgtcatg attgttttac cggcgccagg
     2341 atcatcagct aacaggtaac gcaggggctg cctcgtcagc atttcaccat atacagctgt
     2401 aatctggtgc ggcaagggct ctaccattga agtatgtaca gccaaatatg gatcaaataa
     2461 atgagccaac ttaatcctat atgcttcaga agccagtcta agcatctccc catcagccgt
     2521 aaagctccaa ggcagaccgg cttgaacaat ctcaagcatg ggttctttat ctctatatat
     2581 caattcgctg ttcacattgc ctgaatcatc tttatatgtc agttcaattg caacgtttcc
     2641 tatccatttt gcatcaataa cagtaacatt accattaggc aaaatacctt ttacaactgt
     2701 accagccttt aaatcctcaa gccgcgccat gttttcaccc ctttgttact ttgtaagaat
     2761 cattttcttc tatccgtttt tgaccttgca tcattgggtt tcttggcatc ctttggaatt
     2821 gcccaaatat ttccaagcct cgttgctcct tgaatccgtc catgcgcaca taaaacctgc
     2881 actcggcgga cggtaatatt ccatttttgg gcagcttctt ttgcggtcat atactccatt
     2941 atttcacacc tctaaagtgt ataattaccc aaaaatatta tattcgtaaa tgcgaataat
     3001 ttcaaggaga gttactattc tccatcataa tctttacatc catcctgtct cctcaacccc
     3061 tatgaaaaaa ggccgttatc tattctgtca actcaaccct gctaattttg gagttcaaat
     3121 tttacctcaa aaataatcgt cgatttgttt ccatgtacgt atttttgacc tttaggttga
     3181 gtagacaact tggatgtact tccggaaacc tagtaaaata aaggcttaca gccttgacat
     3241 caatactacc gtctccacat ggtatgtctg tgggaacata tcaaacggtt ggacggagag
     3301 tatttcgtat ttctccttga gtattgccaa gtctcttgca a
//
LOCUS       DAOOJC010000267         3317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_22264_length_3317_cov_3.642244, whole genome shotgun sequence.
ACCESSION   DAOOJC010000267 DAOOJC010000000
VERSION     DAOOJC010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22264_length_3317_cov_3.642244"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1112)
                     /gene="tsaD"
                     /locus_tag="PLA01_07545"
     CDS             complement(93..1112)
                     /gene="tsaD"
                     /locus_tag="PLA01_07545"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPD01183.1"
                     /translation="MKDIIILGIESSCDETSAAVVKNGRQVLSNVISSQVSLHQKYGG
                     VVPEIASRKHVELIIPVIHQGLEEAGITFDQVDAIGVTYGPGLVGALLVGLSAAKALA
                     FALDKPLIGVHHIEGHIAANYIEHNLLEPPFVCLVASGGHSHIVYVEDYDRFEIMGKT
                     RDDAAGEAFDKVARAVGLGYPGGPLIDKAAKYGDSSAIAFPRVVFKDGSLDFSFSGLK
                     TAVLNYLNTMEQKGEKYRVEDVCASFQAAVVDVLTDNLISAAQIKGTGKVALAGGVAA
                     NSLLRSELLNKAQRHGLEFYYPKPVLCTDNAAMVGAAAYYEFIKGKTSDLFLNAVPGL
                     KLGER"
     gene            complement(1351..3027)
                     /gene="lonB"
                     /locus_tag="PLA01_07550"
     CDS             complement(1351..3027)
                     /gene="lonB"
                     /locus_tag="PLA01_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HPD01184.1"
                     /translation="MITTTLFIIQLFFSIIIGLYFLNLYKSQQGNKSAIEKESKKELD
                     KLRKLREIKLTEPLSEKTRPVTLDEIVGQSQGIKALRAALCGPNPQHVIIYGPPGVGK
                     TAAARVILEEAKKTDISPFQRDAKFIEMDATTLRFDERGIADPLIGSVHDPIYQGAGA
                     YGVAGVPQPKAGAVTKAHGGILFIDEIGELHPIQMNKLLKVLEDRRVFFESAYYSSED
                     RNIPYHIHEIFQKGLPADFRLVGATTRTADEIPPAIRSRCVEIYFRPLTPSEIVKISS
                     NAAQKGGFSMEDGCSELVAKYAQNGRDAVNIVQIAGGVALVEGRSQIKRKDIEWVIEF
                     GHYSPRIDKKVIHEEQVGCINGLAVFGNCTGIVIDIEVSAMKVARGKGRVKITGIVEE
                     EEMDTRGQKLRKTSSARSSVDNVLTVLKKVMNIDYEDYDIHLNFPGGIPIDGPSAGIA
                     IATAIYSAIKNLPISSEIAMTGEISIRGKVKPVGGVSAKIEAAKNAGIKKVIIAQENW
                     QEMFENMDIEVIPVKDIYEVMKLVFGKKEGETEGISIQKNAVNMLSASGA"
BASE COUNT          843 a          871 c          565 g         1038 t
ORIGIN      
        1 cccgtggcta gactttcact agctagatta gtgccatgcc tggcacacaa ttaagctgtg
       61 aataaccaaa aagttatcca cagcccttgc ctttatcttt ctccgagttt taatccgggc
      121 acggcattga gaaataaatc ggatgttttg ccttttataa attcgtagta agctgcagca
      181 cccaccattg ctgcgttatc cgtacacaga acaggtttgg gataataaaa ctcaagccca
      241 tgtctttgag ccttattaag aagttcactc cttaaaagcg agttggccgc aactcccccg
      301 gctaaggcaa ccttcccggt tcccttgatt tgtgccgcag aaattagatt gtccgtaaga
      361 acatccacaa ctgcagcctg gaagcttgca cagacgtcct caaccctata cttctcgcct
      421 ttttgctcca tggtgttcag gtaattcaaa actgcagtct taagtccgct aaagctgaaa
      481 tccagactcc cgtccttaaa aacaaccctg ggaaaagcaa tagcactgct gtccccatac
      541 ttagccgcct tgtcaatcag aggtcctccc ggatacccca gtcccacagc ccttgccacc
      601 ttgtcaaaag cctccccagc cgcatcatcc ctggtttttc ccataatttc aaacctgtcg
      661 taatcctcaa cgtatacaat atggctgtgt cccccggaag caaccagaca tacaaagggc
      721 ggttctaaaa ggttgtgctc aatatagttt gccgcaatat gtccttcaat atgatgaact
      781 ccaataaggg gcttgtccag tgcaaacgct aatgctttgg ctgccgacaa acctaccaaa
      841 agggccccca ccagtccggg accataggtc accccaatgg catccacctg gtcaaaggtt
      901 atgcccgctt cctcaaggcc ttggtgtatg accggtatta tcagctcaac atgctttctt
      961 gacgctattt ccggcaccac accaccgtac ttctgatgaa ggcttacctg agacgaaata
     1021 acatttgaaa gcacctgccg tccgtttttc accaccgcag ccgatgtttc gtcacagctc
     1081 gactctattc ccaatataat aatatctttc atttaaacac ccgcttttaa aaaattcaca
     1141 aatatatatt attattttaa tccggttttg taaacaatta tcgggtgcat tgagctacca
     1201 tcactttttt tgttaatttc acttcgtgaa atcccattaa aaaatgtgaa gaagaatgtt
     1261 ctagaaactt aagtttctga aatatctttc ttcacatgaa ttatctctac atgattaatg
     1321 atgcaccctt tcatgagatt aagtaatggt ttatgcccct gatgcactga gcatattaac
     1381 cgcatttttc tgaatggaaa taccctccgt ttcgccttcc ttctttccaa acaccagctt
     1441 cataacctcg taaatgtctt taacgggtat cacttctata tccatgtttt caaacatctc
     1501 ctgccagttt tcttgtgcaa taatcacttt cttaattcct gcattctttg cggcttcaat
     1561 cttagccgat acacctccca ccggtttaac cttgcccctt atggaaatct ctccggtcat
     1621 tgcaatctcg ctgctgatgg gaaggttttt gatcgcagag tatatggcag tagctattgc
     1681 aatacctgcc gacggcccgt caataggtat ccctccgggg aaattcaagt gtatgtcata
     1741 gtcttcatag tctatattca tcactttctt taataccgtt aggacattat ccacagaaga
     1801 ccttgctgaa ctggtcttcc ttaatttctg tccccttgta tccatttctt cctcttctac
     1861 tatccctgtt atcttcaccc tgccttttcc cctagctacc ttcatggcac tgacctcaat
     1921 atcaattaca ataccagtgc agtttccgaa tacagcaaga ccgtttatac agcctacctg
     1981 ttcttcatgg attaccttct tgtcaattcg gggactgtaa tgaccaaact caatcaccca
     2041 ctcaatgtct tttcttttta tctggcttct gccttcaacc agtgcaaccc ctccggcaat
     2101 ctgcacaata ttaacggcat ctcttccgtt ttgagcgtat tttgccacaa gctcggagca
     2161 accgtcctcc atagaaaaac cgcccttctg tgctgcgttg ctgctgattt taacaatctc
     2221 ggaaggcgtc aatggcctga aatatatctc aacacaccgg gacctgattg ccggaggaat
     2281 ctcatccgct gtccgggtcg tagcacccac aagccggaaa tcggccggca aacctttttg
     2341 aaatatctca tggatatgat atggaatatt cctatcctcc gagctgtaat atgcactctc
     2401 aaagaatacc ctccggtcct ccaatacctt taggagctta ttcatctgta tgggatgaag
     2461 ttctcctatt tcatcaataa ataatattcc accatgagcc tttgttacag ctcccgcttt
     2521 gggctgcggc acaccggcaa ctccgtatgc tcctgctccc tggtatatcg ggtcatggac
     2581 agaaccaatc aacgggtctg caattcccct ttcgtcaaac cttagtgttg ttgcatccat
     2641 ttcaataaat ttcgcatccc tttggaaagg agatatgtcg gttttctttg cctcctcaag
     2701 tatcactctg gcagcagcgg ttttccccac tcctggcgga ccgtaaatta tcacgtgctg
     2761 cggattcggt ccgcacaagg ctgcccttaa ggctttaatg ccctgactct gccccactat
     2821 ttcatccagc gtaacgggcc ttgttttttc cgatagaggc tctgtcagct ttatttcccg
     2881 caactttctc agcttatcca gctctttttt cgattccttt tcaattgcac tcttattgcc
     2941 ctgctgcgat ttatatagat ttagaaagta caatcctatt ataattgaga aaaaaagctg
     3001 tatgataaaa agagtggttg taatcattgt ttgtcctccc caaaagatct atatttttgc
     3061 gttcttcata ctgtttaaat tattgccagg ttggtggttc taatctgtga aacccctcta
     3121 aatcctatgt cctgatgctg tttacctcat tttatatact tcgccttttt atcctgcata
     3181 ttcatagctt aatttaaaat gttgcattta tagtatttac tgtgttaggt attttatcct
     3241 gatatttaaa aagatttttt cgaaacaaaa tgttattcat atttagaaaa tactatatac
     3301 ttctattcta caaaact
//
LOCUS       DAOOJC010000268         3316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_22269_length_3316_cov_3.012266, whole genome shotgun sequence.
ACCESSION   DAOOJC010000268 DAOOJC010000000
VERSION     DAOOJC010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22269_length_3316_cov_3.012266"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="PLA01_07555"
     CDS             complement(<1..1501)
                     /locus_tag="PLA01_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HPD01185.1"
                     /translation="MLLNHFAKCFGKGLIFVAFIALLSWGFVGVSSAEETDETRKIVT
                     TDFVDLKFTINKNNYTSKKVSKKLEVAPYIVNGRTLVPFRTIFEELGYDIVWDDATKT
                     ITATYKGNVIKLEINNKKAIVDGKEVVLDVAPEIVKSRTVVPLRFVAENSGSFVNWDP
                     DEKSITISRIGEFSTGTVLFYDQNGRIPMVYIYDGSKIESISLDGNEIKNAITYNGGL
                     LITLFDKENDTNNLVTYRNGKFDVLISNFEIKEKVEFNNNLLLHGYDRARKKDSLYRF
                     DGRNIYLIEDNFAMGRYEIFNDKLVINKYNDLRKYSLLVFDKSSWTPKLLQDDFIIKE
                     SIIEDNTLFISAIGSAGNSKPFACYNGTEFRILHKDLDIDLSRTIQFRSKDDINNIVT
                     VAKKDGKYYFLVLRNTSKNTSYYDVYDLFVPNSVFTDVGSKAGTVTVEGIKDYNGKMF
                     ISFKQTNSVITSDSFRVIGLPSGTKPESTFTGSKYLVLDNCKTIYKNVLY"
     gene            complement(1964..>3316)
                     /locus_tag="PLA01_07560"
     CDS             complement(1964..>3316)
                     /locus_tag="PLA01_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPD01186.1"
                     /translation="TSFNNPALAGLNSFELFNFMLENFKRVGIKVILDVHSATTDNQG
                     HTYPLWYNGTITEEVFKSAWVWVANYYKNDDTIIGYDLKNEPHTNTGNVKIKAQSAIW
                     DDSNHPNNWKRVAQETALAILNVHPNALIFVEGIEMYPKDGIWDDETFDTSPWTGNND
                     YYGNWWGGNLRGVRDYPIDLGKYQSQLVYSPHDYGPLVYEQEWFKGPFTTADDREAKK
                     ILYEQCWRDNWAFIMEEGISPLLIGEWGGMTEGGDKLLDDNKKYLRCMRDYIIENKFK
                     LHHTFWCINIDSADTGGLFTRDEGSPFPGGRDLKWNDNKYDNYLYPTLWKTEDGKFIG
                     LDHKVPLGNNGVSIGNLSGNPTPTTAKPTPTKKIDPTPTDIPATPTEGNVKYGDINDD
                     GDIDSTDLTMMKRYIMRKLQNINTEAADVNLDGDINSTDLSLMKRYILKKINKLPYVT
                     "
BASE COUNT          980 a          696 c          509 g         1131 t
ORIGIN      
        1 tgtaaagcac atttttatat atggttttac aattatccaa caccaagtat ttagaacctg
       61 taaacgtact ttcaggcttt gtgcctgaag gcaagccaat aaccctgaag ctgtctgaag
      121 taattacact gttggtctgc ttgaaggaaa taaacatttt tccattatag tccttgattc
      181 cttccaccgt cactgttcct gcttttgaac ccacatccgt aaatactgag tttggaacaa
      241 acaggtcata aacatcataa tatgatgtgt tcttactagt atttctaagt actaaaaagt
      301 aatatttccc atctttcttt gcaactgtaa ctatattgtt tatatcgtct ttactcctga
      361 attgaattgt tctgctaagg tcgatatcaa ggtctttatg caaaattctg aactcagtcc
      421 cattgtaaca tgcaaagggc ttactgtttc ccgctgaacc aatagcactt ataaaaagag
      481 tattgtcttc aattatcgac tctttaataa taaaatcatc ctgcaacaat ttcggagtcc
      541 atgaggattt atcaaaaacc aaaagagaat attttctaag gtcattatac ttgtttataa
      601 ctaatttgtc attaaaaatt tcatatctgc ccatcgcaaa gttgtcttca atcagataga
      661 tattccggcc gtcaaaccta tataggctat ccttctttct tgccctgtca tatccgtgca
      721 gcaataaatt gttattaaac tcgacctttt cctttatttc aaaattactt atcaacacat
      781 caaatttccc gtttctatat gttacaaggt tgtttgtatc attttccttg tcaaacaggg
      841 ttataaggag accaccatta taagtgatag cattttttat ttcatttccg tcaagagaaa
      901 tggactctat cttacttcca tcgtatatat ataccattgg aattctccca ttttgatcgt
      961 agaaaagaac cgtacctgta gaaaactccc ctatcctgct tattgttata cttttctcat
     1021 cgggatccca gttaacaaaa gagcccgaat tctcagccac aaatcttaag ggcactacag
     1081 ttcttgattt tacaatttcc ggtgcaacat caaggacaac ctcttttcca tctacaattg
     1141 cctttttatt atttatttca agctttatga catttccctt gtatgttgcc gtaattgttt
     1201 tagtagcatc gtcccagacg atatcatacc ccagttcctc aaaaatcgtc ctgaaaggaa
     1261 ccagcgttct tccgttaaca atataaggag caacctccaa cttcttagat acctttttag
     1321 aagtgtaatt attcttgtta attgtaaact tcaaatcaac aaaatccgtt gtaacaattt
     1381 ttctcgtctc gtcagtctcc tctgctgaag ataccccaac aaagccccat gaaagtagtg
     1441 caataaaagc aacaaaaatc aaaccttttc caaaacactt tgcaaaatga ttcaacaaca
     1501 tttttccccc tcctgattag aaaataaata agacatatca ttatacatta taacattaaa
     1561 ttaaacaata aacttataag aaaaatgtta tcatatcaga gtattcaagt caatcccaag
     1621 aatgtcatta atatctttgc ataagattat tattaaccgc caataatatt tagcataggg
     1681 tccatgatgg tcgggcgaaa actgttataa tgtcagaaaa ggctttataa tggtcggcta
     1741 acggctaata ccgggttaat cctgttgata ttatagcctg ttattagccg gcaggaagag
     1801 tattatatgt cctgtaaggc tatactgcag cactaaatgt tgcagtgtgg ttaaaaggga
     1861 tgtatagaaa aaataaaagc gaaggacctg tggtcacatc cgcttttatc ttaatattat
     1921 tgtttatatt attcttctgc caattatcca tcctattata ttgtcaagta acataaggca
     1981 gtttattgat tttcttcaat atgtaccttt tcatcaatga taggtctgtt gaatttatgt
     2041 caccatcaag gttcacatcc gctgcctcag tattgatgtt ctggagttta cgcattatat
     2101 accttttcat cattgtcaag tctgttgaat caatatcacc gtcatcattg atatcaccat
     2161 atttgacatt gccttcagta ggagtagcag gaatgtcagt gggtgttgga tcaatcttct
     2221 ttgtcggagt aggtttagca gtagttggag taggattacc tgacaaattc cctatagaca
     2281 caccattatt tccaagaggt actttatgat ccaaaccgat aaatttaccg tcttcagtct
     2341 tccacaatgt aggatataaa taattatcgt atttgttatc attccacttg aggtctcttc
     2401 ctccggggaa tggggatcct tcgtcccttg taaacaatcc gccggtatct gccgagtcta
     2461 tattgatgca ccagaaagta tggtgtaatt tgaatttgtt ttcaataata taatccctca
     2521 tacatctcaa atatttttta ttgtcgtcaa gaagcttgtc gcctccttct gtcataccgc
     2581 cccactcacc tatcaataac ggagatattc cttcttccat tataaatgcc cagttatctc
     2641 tccagcattg ctcataaagt atttttttag cttctctatc gtcagcggtt gtaaaaggcc
     2701 ccttaaacca ttcctgctca tataccaatg gaccgtaatc gtgcggagaa taaacgagtt
     2761 ggctttgata ttttccaaga tctataggat aatctcttac ccctcttaaa ttaccgcccc
     2821 accagttgcc gtagtaatca ttattccctg tccatgggct tgtgtcaaag gtctcgtcat
     2881 cccatatacc gtctttagga tacatttcaa taccttcaac aaatatcaag gcattggggt
     2941 gtacgttcag tattgccaat gcagtttcct gagctactct cttccagtta ttcggatgat
     3001 tggaatcatc ccatatagca ctctgtgctt taatttttac atttccggta ttggtatgag
     3061 gttcgttttt aaggtcatag ccaataatcg tatcatcatt tttataataa ttggcaaccc
     3121 acacccacgc tgatttgaac acttcttccg ttatggtacc gttataccag agaggatatg
     3181 tatgtccctg attatctgtt gttgcactgt gtacatcaag tataaccttg attcccactc
     3241 ttttgaaatt ttccagcata aaattaaaca actcaaaact gtttaatccc gctaaagccg
     3301 ggttattaaa gcttgt
//
LOCUS       DAOOJC010000269         3306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_22361_length_3306_cov_4.224546, whole genome shotgun sequence.
ACCESSION   DAOOJC010000269 DAOOJC010000000
VERSION     DAOOJC010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22361_length_3306_cov_4.224546"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2209
                     /locus_tag="PLA01_07565"
     CDS             <1..2209
                     /locus_tag="PLA01_07565"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD01187.1"
                     /translation="KDSLNIIRELIENLDLINITFLLASRYEVEEFSGTKSLKYHTLK
                     LRTLSKSAVKQLMCKLLQCKNIDKNLLDAAVKFTNGNPLYIREFIQNIKKSDTFQVVE
                     DVAVIDAKTITSLPNNIQNIILANLSEMSDKERRLLEVASVIGRDFSLTILSAILEMG
                     RNEVNDLLRLPVHMNLISLKSAYTSSGVVEKVFVFNQDMEREVIYDSILNKEKKELHK
                     KIAEFIEEKYARELEEYYEILFTHFHKAGQLKKAAEYSFKTAIKNKDNFNFTSSLEYY
                     DRFLLLVKEEDDDQINPKILEAYKDMGYIKFIMADYELSMFYLNKALKKAKLYDDIYS
                     IRIMIAAVYKEQDMYEDALKILDDIQLKINKENTLYGRLLQMKCSILRILGSTEALTL
                     AQKSEKLLIKMKDYENLAETMNQAGIIYYIRGDIADALFCLDKSYKYAEKVNNLAIMT
                     KVSGNMGAFYHAIGMISKAQDFLNRSISISQKISDQQGCVAGSINLGILYMDKGLFNE
                     AETLFNKALENSREISSRLNESIAITNIGDILYERGLFDQAISHYNKSLEIAREINVP
                     LGEGINYLSIAKLDLDRNLLDKAEKMLGIVLKIFTEVEEMSYLCDYYRYMAICEMKKG
                     NPEDALNNCDKAIEISREVKSNIRRLKAIRIKAGILACMDKNEEAIELLSESINMSHQ
                     LESDYEAAKGYFERFKLYRKMEEMDKAAVDIKLAKECIGKVDDCKLKEVIEKA"
     gene            complement(2268..3221)
                     /locus_tag="PLA01_07570"
     CDS             complement(2268..3221)
                     /locus_tag="PLA01_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPD01188.1"
                     /translation="MNQNFLFEDFKIIGTNTAKYLESFKDLLLTSSGVLISGETGTGK
                     ELAARWIHFNDPKRNNNPFVKITIPNIGENIFESEIFGYEKGAFTGAVSSKKGLIEVC
                     EEGSLFFDEIENASPSIQSKLLQLFEDRLYRKVGDTSYRKTNARFIVAANKDLYEEVQ
                     KNSFRQDLYFRLAVIELKMPPLRERGRDILEIANFYIEKYSKELGRNVRLLTQKDLPR
                     LCSYPWPGNVRELKNFIERSVVFSKDSRIDLSKLEKAVPPIETAPEEIYTYREYIRLK
                     EQELFVKALSMCNGNVEQTASLIKMSVPFVYKKIKELGIRL"
BASE COUNT         1058 a          563 c          703 g          982 t
ORIGIN      
        1 taaggattca ttgaatatta taagagagct tattgaaaac ctggacctta taaatatcac
       61 ttttttattg gcttcccggt atgaggttga ggagttcagt ggcactaaat cattgaagta
      121 tcatacttta aaacttagga ccctgtctaa aagtgcagta aaacagttga tgtgcaaatt
      181 gctccaatgt aaaaacattg ataaaaacct tctggacgct gctgtgaagt ttaccaacgg
      241 aaatcccttg tacattcgag aatttattca aaatattaaa aaatccgata cattccaagt
      301 ggttgaagat gttgcagtta tagatgctaa gacaattaca tcccttccta acaatatcca
      361 gaatattata ttggcaaact tgtccgagat gagtgataag gagagaagat tacttgaggt
      421 tgcatcggtt atcggcaggg acttcagcct tacgatattg agtgcaattc ttgaaatggg
      481 aaggaatgag gttaacgact tgttgaggct ccctgtacac atgaacttaa tttccctgaa
      541 gtctgcgtat acctcctccg gtgttgtgga gaaagtgttt gtgttcaacc aggacatgga
      601 gagagaagtt atttatgata gtattttaaa caaggaaaag aaggaactgc ataaaaagat
      661 agcggaattt atagaggaga aatatgcccg ggaattggaa gagtattatg agattctctt
      721 cactcatttt cataaggccg gacagctcaa aaaggcagcg gagtatagtt ttaagacagc
      781 cataaaaaac aaagacaatt tcaatttcac aagttcactg gaatactatg accgttttct
      841 cctattggtc aaagaagagg acgatgatca aatcaacccc aagatattgg aagcctataa
      901 agacatgggc tacataaaat ttataatggc tgactatgag ctttccatgt tttatttaaa
      961 caaggcttta aagaaagcaa agctttatga tgatatttac tcaatccgga taatgattgc
     1021 agcagtttat aaagaacagg atatgtatga ggatgccctt aaaattcttg atgatattca
     1081 gcttaaaata aacaaggaaa atacacttta tggaagactt ttgcagatga aatgcagcat
     1141 attgaggatt cttggcagta cggaagcact cactttagcc caaaagtccg agaagctgct
     1201 cataaaaatg aaggattatg aaaaccttgc cgagaccatg aatcaagccg gaataatata
     1261 ctatatccgg ggagatatag ccgatgcctt attttgtctt gacaagtcat ataaatatgc
     1321 tgaaaaggta aataaccttg ctatcatgac caaggtgtcg gggaatatgg gtgcatttta
     1381 tcatgctatc ggtatgattt caaaagcaca ggatttttta aacagatcca ttagcatctc
     1441 ccaaaaaata tcggaccagc aggggtgcgt ggcaggaagt ataaatcttg ggatacttta
     1501 tatggacaaa ggtcttttca atgaggctga aactttgttc aacaaagcat tggagaactc
     1561 ccgtgaaata tcttcaaggc ttaatgaaag catagcaata accaatatcg gtgacatatt
     1621 atatgaaaga ggcctttttg atcaggcaat atcccactat aacaaatctc ttgaaatagc
     1681 ccgggagatt aatgtaccct taggagaagg aataaattac ctgagcattg cgaaacttga
     1741 tttagacaga aatttgctgg acaaggcaga aaaaatgctt ggtattgtat tgaagatttt
     1801 tactgaagtt gaggaaatgt cctatttatg tgattactac aggtacatgg caatatgtga
     1861 gatgaaaaaa ggaaacccag aagatgcgtt gaataattgt gataaagcta ttgagatatc
     1921 ccgggaggtt aagagcaata taagaaggct taaggcaata agaataaaag caggaattct
     1981 tgcctgtatg gataaaaatg aggaagccat tgaactgctt tcggaatcaa taaacatgtc
     2041 tcatcagctt gagtcagact atgaagcagc caagggatat tttgaaaggt ttaaattata
     2101 caggaagatg gaagaaatgg ataaagccgc ggttgatata aaactggcta aagagtgcat
     2161 tggaaaggtg gatgactgca aattgaaaga ggtaattgag aaagcctgat ctcaaggtac
     2221 agtggcaagg atgaataaag atattccaga aacttaagtt acgcaaacta gagccgtatc
     2281 cctaactctt ttattttttt gtaaacaaag gggactgaca tttttatgag ggatgcggtt
     2341 tgttccacat ttccgttgca catggacaga gcttttacaa agagttcctg ctctttaagc
     2401 cttatatatt ctctgtaggt ataaatctcc tcaggagcgg tttcgattgg agggacggct
     2461 ttttcaagtt tggatagatc aatccggctg tcctttgaaa agacaacgga tctctcaatg
     2521 aagtttttta attcccgtac atttccgggc caaggatagg agcagagccg gggtaaatct
     2581 ttttgagtca gaagcctcac gttccttccc aattcttttg aatatttttc tatatagaag
     2641 tttgctattt caaggatatc ccttcccctt tcccttaatg ggggcatttt gagttcaata
     2701 acagcaagtc tgaaatacag gtcctggcgg aaactgtttt tttgcacttc ctcgtatagg
     2761 tctttgttgg cagcaacaat gaatcttgca ttggtttttc tataggaggt gtcacccacc
     2821 ttgcggtaga gcctgtcttc aaagagttgc agcagcttgc tctgtatgga tgggcttgcg
     2881 ttttcaattt cgtcaaagaa aaggcttcct tcttcgcata cttcaatcag tcctttcttt
     2941 gagctgacgg cacctgtaaa agcccccttc tcatagccga aaatctcact ttcaaatatg
     3001 ttttcgccta tgttgggaat tgtaattttt acgaaaggat tgttgtttct cttggggtca
     3061 ttaaaatgaa tccaccttgc cgcaagttct ttaccagtac cggtttctcc ggaaataagt
     3121 acacccgagc ttgttagaag gaggtcttta aagctctcta aatactttgc tgtgttggtt
     3181 ccgatgatct taaagtcttc aaaaagaaag ttctgattca taagtgtttt cccccttagt
     3241 ttatatatcg gtagttgttt gtaaaatgct gtaacccttg aaaccaaagg gttttagcag
     3301 tatatt
//
LOCUS       DAOOJC010000270         3305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_22376_length_3305_cov_3.219077, whole genome shotgun sequence.
ACCESSION   DAOOJC010000270 DAOOJC010000000
VERSION     DAOOJC010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22376_length_3305_cov_3.219077"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..720)
                     /locus_tag="PLA01_07575"
     CDS             complement(67..720)
                     /locus_tag="PLA01_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPD01189.1"
                     /translation="MIKKITAMLITAVMSICFLVTPAFADYYSDKKTLYRGMSSSNVT
                     ELQKDLRDLGYFRQNPTGYFGSITYQSVINYQRDNNLAVDGIVGRQTARELKVDKVLQ
                     TAKSYQGVPYVWGGTSPSGFDCSGFTHYVMLKNGIIIQRTASAQYNEGSPVSKSNLKP
                     GDLVFFSTYKKGPSHVGIYVGNNKFIHASSGAGKVVISDLNTSYYVQHYIGARRIIN"
     gene            996..2543
                     /gene="spoVB"
                     /locus_tag="PLA01_07580"
     CDS             996..2543
                     /gene="spoVB"
                     /locus_tag="PLA01_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein B"
                     /protein_id="HPD01190.1"
                     /translation="MAKKSFVGSAIILMIASFVVRILGFIYRIYLSNLIGSEGMGLFQ
                     LISPVYSLIILTLTSGISIAVSKMVAEEMAKNHQVNLRRITACALGIVVSMGAVVSLL
                     MFIFIDPVVNIILKDSRTYYSMLLLIPCIPVISAASAIKGYFYGIQDVIPTACSQIVE
                     QIVRTGLVMAMAGFFANIGLEYACAIATVGMSIGEISNLLVLVIVYRLRKAKSSKSIS
                     KKGFMRKRVMIKELLKVSVPVSFNRFITSIMATIEYILIPRMLLLAGMNYQSSIQEYG
                     KLTGMAMPLIFFPTLVTSALATTLVPAISEAISVRSYKTVNYRISKSVQLTFMMGFIF
                     TVLFMNYSQEIGDLVYRRENIGNILYLLSFTSIFIYLQQTLLGILNGLGKQGISLRNS
                     IVGYIIRIAFVIYCIPAYGISGYITGMIISSICVCCLNIATVVKTTGMILDFRNWVLK
                     PGLVGAIMFFIGKYIESFFTIFNLEYTWTVVLTIFGNVITAFSLMFVLGALDREETLK
                     LLGLKKS"
     gene            2821..2994
                     /locus_tag="PLA01_07585"
     CDS             2821..2994
                     /locus_tag="PLA01_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01191.1"
                     /translation="MDDYARQLENKIKEKNRLIDYYNQKLDIDESKVKNVEAELDSLL
                     YEYYKTTLKEKTD"
BASE COUNT         1012 a          586 c          665 g         1042 t
ORIGIN      
        1 gtacggttca acgagaggga aataattctt agcaattatt tctctactct atttatcagg
       61 tgcatattag tttataattc ttcttgcacc gatataatgc tgtacataat agctcgtgtt
      121 caggtcggat attactactt tacctgcacc ggagcttgcg tgtataaact tattgtttcc
      181 aacatagatt cctacatgtg aaggtccttt tttatatgtt gaaaagaata ccagatcccc
      241 cggcttcaag tttgattttg ataccgggct accttcgtta tactgtgcac tggctgtcct
      301 ttggattatt attccgttct taagcattac gtagtgagta aagccggagc agtcaaatcc
      361 tgagggtgat gtaccacccc acacataagg aactccctgg taactttttg ctgtctgcag
      421 tactttatcc acctttaact ctctggcagt ctgccgtcct acaattccat cgacagcaag
      481 gttgttatcc ctttgataat taattaccga ctggtaagtg atactaccga aataacccgt
      541 tggattttgg cggaaatatc ctaaatcccg caggtctttc tgaagttctg taacattgct
      601 gctactcata ccacggtaca atgttttttt gtctgaataa taatccgcaa atgccggagt
      661 aacaagaaag cagatcgaca taacggctgt aatcagcatt gcagttattt tctttatcat
      721 aaagatcccc ctttcaaaag cctgatttaa tccctggttc aggcttgctt attctggtga
      781 ccgtcacagt gactcattat aagggagggt atgggcatta atcaacctgt aatatatggg
      841 ataatctata gaaagaagga gacacaggat tttgaggcaa aattaaagag aagaaataca
      901 cagaagtaat gtgccaatta aattaacggg attggtcata agttgttcaa tcagtaaata
      961 ttaataatca ggacaattta attttcagga ctaatatggc taaaaaatcc tttgtcggta
     1021 gtgctataat tttgatgata gcgagctttg tagtaagaat tcttggcttt atatacagaa
     1081 tatacttatc gaaccttatc ggttcggaag gaatgggtct gtttcagctc atttctccgg
     1141 tctattcctt gattatactt actctgactt cagggatatc catagcggta tccaaaatgg
     1201 tagcagagga gatggcaaaa aatcatcagg taaacctgcg gcgaataacc gcatgtgctt
     1261 tgggcattgt tgtatccatg ggtgcggtgg tatccctgtt gatgtttata tttatagacc
     1321 cggtagtgaa tattattttg aaggattcga gaacctatta ttcaatgctt cttctaatac
     1381 catgcatacc ggtaatttct gcagcatctg caataaaggg atacttttac ggtatacagg
     1441 acgtaatacc cacggcttgt tcgcagattg tggaacagat cgtaaggacg ggtcttgtca
     1501 tggccatggc aggctttttt gcaaatatcg gactggaata tgcctgtgcc attgcaactg
     1561 tgggcatgtc tattggagaa atatcgaacc tgctggtttt ggttatagtt tacaggctta
     1621 gaaaggccaa gtcctctaag agcatttcaa aaaaaggttt tatgagaaag agggttatga
     1681 ttaaggaact tttaaaggtt tctgttcccg tgtcgttcaa caggtttatt acatcaatta
     1741 tggctactat agagtatatt ctgattccaa ggatgcttct gctggcagga atgaattatc
     1801 aaagcagcat acaggaatat gggaagctga caggaatggc tatgccgtta atattctttc
     1861 cgacacttgt gacttctgcc ctggcaacaa ctcttgttcc tgcaatttcc gaggctatat
     1921 ccgtaaggag ctataaaacc gtgaactaca gaatatccaa gtcggtgcaa ttaaccttca
     1981 tgatggggtt tatatttaca gtgctgttca tgaattattc tcaagagatt ggagatcttg
     2041 tttaccgcag agagaatata ggaaacatac tgtatcttct ttcatttacc agcatattca
     2101 tttatcttca gcaaaccctt ctcggtatat tgaacggact tggaaaacag ggcatttctc
     2161 ttagaaactc tattgtcggg tacataataa gaatagcctt tgtaatatac tgcattcctg
     2221 catatggtat cagcggctac ataacaggca tgattataag ttcaatatgc gtatgctgcc
     2281 tcaatattgc cacagtagtg aaaacaaccg ggatgatact tgacttcaga aactgggttt
     2341 taaaacccgg attggtgggt gcaataatgt tttttatagg aaaatatatt gagagcttct
     2401 ttacgatttt taatctggag tatacatgga ctgtcgtact tactatattt gggaatgtca
     2461 ttacagcctt ttcattaatg tttgttttag gtgccctgga tagggaggaa accctaaaac
     2521 ttctcggact taagaaatcc tgatgaattg cacgaaacaa aaaattaccg gcactctaaa
     2581 ggggattcct aaaaaattta ataattttat gaaattaata taaatttatt attgcttttt
     2641 gccgatatta tattgacgtt gtttggttcg tataacaata ttaaatttat attaacaata
     2701 tgtgaaattt ttgttaaatt attgagaggt cggctttctt attgtataat aaaactaatg
     2761 actttactaa tgactatttg cttttttttg tgggagtagc tgttaaggag gtgttgcaca
     2821 atggacgatt acgcaaggca gctagaaaac aaaataaaag agaagaacag gcttattgat
     2881 tattacaacc agaaacttga catagacgag tcaaaggtaa aaaatgttga ggccgagctt
     2941 gacagtcttt tgtacgagta ctacaagaca acactaaagg aaaaaacgga ctaaagtaat
     3001 tctatagcca tataaatacc aaagaccggt aagctggtaa ttatgccctt gccggtgaat
     3061 tacatagggt tgttctctct ccagcttctt ttgaacatat tatgaaaaac aaacgggcat
     3121 tagttggtca gtgtctttgc ataaagtaaa ctgaaatata tggaatgttt caatatccgc
     3181 atccttcaag cctccacaaa atcaaacatg tgaataaata agagaatatg cagcagagag
     3241 agccctggaa atcaaaacag gacaagacgt taataaaccc ggagatttat cttaataagg
     3301 tacat
//
LOCUS       DAOOJC010000271         3295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_22460_length_3295_cov_3.259877, whole genome shotgun sequence.
ACCESSION   DAOOJC010000271 DAOOJC010000000
VERSION     DAOOJC010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22460_length_3295_cov_3.259877"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PLA01_07590"
     CDS             complement(<1..906)
                     /locus_tag="PLA01_07590"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HPD01192.1"
                     /translation="MAFINENYLKLQGSYLFSEIAKRVNKFKEENPDAKIIRLGIGDV
                     TRPLAPAVIEAMHKAVDEMAKEETFRGYGPDQGYSFLINKIIENDYLPRGIKLSEDEV
                     FVSDGAKSDTGNIQEIFGLNNTFAVTDPVYPVYVDSNVMAGRTGVYTEDGHFERITYH
                     PCTAENNFIPEIPKHKVDMIYLCFPNNPTGMTLSKEELKKWVDYAKENKAVILYDSAY
                     EAFIREDDVPHSIYEVEGAKEVAIEFRSFSKTAGFTGTRCAYTVVPKEVMAYTSNGEP
                     YPLNKLWNRRQSTKFNGVPYIIQQGA"
     gene            complement(921..1754)
                     /gene="dapF"
                     /locus_tag="PLA01_07595"
     CDS             complement(921..1754)
                     /gene="dapF"
                     /locus_tag="PLA01_07595"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPD01193.1"
                     /translation="MKFTKMHGLGNDYIYVNCFRETVEDPSKVAKFVSDRHFGIGSDG
                     LVLIMPSDKADFRMRMFNSDGSEAEMCGNAIRCVGKYVYDNGLTKKNVVSIETLAGIK
                     ILELTIKNDSVELVRVDMGEPILKPVNIPVLSDKEIFKAEPVTVNGREFKVTCVSMGN
                     PHAVSYVESVDDFPLEQIGPKMETHNLFPRRINAEFVEVIDRTTLKMRVWERGAGETL
                     ACGTGACAVLVASVLNGISERKATVKLLGGDLIVEWDENNNHVYKTGPAVKVFEGEIE
                     I"
     gene            1930..2628
                     /locus_tag="PLA01_07600"
     CDS             1930..2628
                     /locus_tag="PLA01_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD01194.1"
                     /translation="MKILIIIPAYNEETTIQKVIRHITLKMPEADILVVNDGSSDETS
                     KRASQAGAMVVDLPYNLGIGGAMQTGYIFAKENDYDIAVQVDADGQHDPSYIKDLIRP
                     LIDNTADVAVGSRYISKTGYRSSLFRRIGMVFFSFLVSLLTNQKFKDTTSGFRAVNKD
                     VIHCFASHYPDDYPEVDVLVKLKKKNFRIVELPVQMQERQGGKSSITALRSLYYMVKV
                     SLSLIIGALRSSEE"
     gene            2644..2988
                     /locus_tag="PLA01_07605"
     CDS             2644..2988
                     /locus_tag="PLA01_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HPD01195.1"
                     /translation="MAVNIYSLSIMFSILFLLTVIELVRKNKLQERYSLLWIFISIVF
                     LVLSSTSGLLNHLAKILDIKNPPSLLFLLGLIYLIVYSLNTTVVVSKQNERITRLTQE
                     LSLLKQKMSNNK"
BASE COUNT          904 a          737 c          561 g         1093 t
ORIGIN      
        1 cgccccctgc tgaattatat atgggactcc attgaactta gtagactgtc ttctgttcca
       61 gagtttgttc aacgggtaag gctcaccgtt gcttgtatat gccataacct cctttggcac
      121 aacagtgtat gcacatctcg tacctgtaaa tccagctgtt ttggaaaaac ttctgaattc
      181 aatagcaact tcctttgcac cctctacttc atatatgctg tggggtacgt catcctcccg
      241 gataaatgcc tcataggctg aatcatacaa tattacagcc ttgttttcct ttgcataatc
      301 cacccacttt ttgagctctt cttttgaaag ggtcataccc gtcggattgt tggggaaaca
      361 caggtatatc atatccacct tgtgcttggg gatttcaggt ataaagttgt tttcagcagt
      421 acagggatgg taggttatcc tttcaaagtg tccgtcttcc gtatatactc cggtccttcc
      481 tgccatgaca ttactgtcta cataaacagg gtatacggga tcggtcaccg caaatgtatt
      541 gttaagcccg aatatttcct gtatgttccc ggtgtcactc ttagcaccgt cacttacaaa
      601 aacctcatct tcacttagtt ttattccccg gggaagataa tcgttttcta ttattttatt
      661 tattaaaaaa ctgtatccct ggtcaggacc gtaacctcta aaagtttcct cctttgccat
      721 ttcatccact gccttatgca ttgcttcaat gacagccggt gccaaaggtc ttgttacgtc
      781 tcctatcccc agccttatta tttttgcatc ggggttttct tctttaaact tatttactct
      841 ttttgctatt tcagaaaaga gatagcttcc ctgaagcttc agataatttt cattaataaa
      901 tgccatatta atcttccttt ctaaatttct atttcgcctt caaatacttt tacagcaggt
      961 cctgttttgt atacgtgatt gttattttca tcccactcca caataaggtc tccgcccaat
     1021 agcttcactg ttgcttttct ttcacttatg ccgtttaata ccgaagctac cagcacagca
     1081 caagcacctg tgccgcatgc caaagtctcc cctgctcctc tttcccatac cctcattttc
     1141 aacgtagtcc tgtcaataac ctcaacaaac tccgcattta tccttctggg aaagagatta
     1201 tgagtttcca tcttgggtcc gatctgctcc agcggaaaat catctacact ttcaacataa
     1261 gagacggcat ggggatttcc cattgacaca catgttactt taaactctct tccgtttacg
     1321 gtaacaggct ccgccttaaa tatttctttg tcactcaata ccggtatatt cacaggcttt
     1381 aatataggtt ctcccatatc aacccttaca agctccacac tgtcattttt tattgttagc
     1441 tcaagaattt ttatccctgc cagagtctca atactaacta cgttcttttt ggtcagaccg
     1501 ttgtcataca cgtacttacc gacacatctt atagcatttc cgcacatttc cgcttccgaa
     1561 ccgtcagaat taaacatcct cattcgaaag tccgctttgt ctgaaggcat tatcagcaca
     1621 agtccgtccg aaccgatacc aaagtgcctg tcactaacaa atttcgctac ttttgaagga
     1681 tcctcaacgg tctctctaaa gcaatttaca tatatatagt cattgcctaa accgtgcatt
     1741 ttggtaaact tcatattgca acctcccaat gccaatcttg cattttcaat ctccttacat
     1801 tataacatac attttttgca catcaagtgc atttattatc agtttttgat acagaatata
     1861 tttatatcaa agttgtactt tcacaggagg tgtaaaaggg tgtatacggt ttaatacacc
     1921 cttgtaataa tgaagatatt gattattatt ccggcttata acgaggaaac cacaatacaa
     1981 aaggtcatac ggcacataac ccttaaaatg cctgaagcgg atatacttgt ggtaaatgac
     2041 ggttcttccg atgaaacttc aaaaagagct tcccaagccg gtgccatggt agtggacctt
     2101 ccttacaatc ttggaattgg gggtgccatg cagacaggct atatttttgc caaggagaat
     2161 gattatgata tcgcggttca ggttgatgcc gacggacagc acgatccttc ctatataaag
     2221 gatctaataa ggcctcttat cgacaatacc gccgatgtag cagtcggatc aaggtacata
     2281 agcaagaccg gctacagatc gtctttattc agaagaatag gaatggtctt cttctcattt
     2341 cttgtgagtt tattgacaaa ccagaaattc aaggacacga cttcgggatt tcgggctgta
     2401 aacaaggatg tgatacactg ttttgcaagt cactatcccg atgattatcc ggaagtggat
     2461 gttctggtaa agcttaaaaa gaaaaatttc agaatagtcg agcttcccgt tcaaatgcaa
     2521 gaacgtcagg gaggaaaatc ttccattacc gccttacgtt cattatatta catggttaag
     2581 gtcagtcttt cccttataat aggtgcatta cgttcttccg aggaatagaa aggtgtgttt
     2641 tttatggctg taaacatata ctccctatcc ataatgttca gtattctttt tttattgact
     2701 gtgattgagc ttgtaagaaa aaacaagctt caagagcgtt attccctgct ctggatattc
     2761 atcagcattg tttttcttgt cctttcaagc acctccggtt tgttgaacca tctggcaaag
     2821 attcttgaca ttaaaaatcc cccttcctta ctgtttttgc ttggtcttat ttatctgatt
     2881 gtttacagtc tgaacaccac tgttgtcgta tcaaaacaaa atgaaaggat aacccgccta
     2941 actcaggaac tctctcttct caagcagaaa atgtcgaata ataaatgaag tattatagca
     3001 taatttataa attgcttatt atcctcaatc ctgctataat atacaatata ctgttgtatt
     3061 ttttgctttt ttgttgaggt tggaaatcct ttgccgcctc gctgtaattt ggggtatttt
     3121 ttatatcata atcaggattt ttgtttcctt gcaagaaatg ccgacggttt cgaacaggag
     3181 tatattatga ataacaatct tatttcagtt gctattcccg tatataatga agggaagcag
     3241 atatacgaaa acattaatgt aatatacaaa attatgactg aatacaatat taatc
//
LOCUS       DAOOJC010000272         3274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_22647_length_3274_cov_3.772600, whole genome shotgun sequence.
ACCESSION   DAOOJC010000272 DAOOJC010000000
VERSION     DAOOJC010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22647_length_3274_cov_3.772600"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLA01_07610"
     CDS             complement(<1..309)
                     /locus_tag="PLA01_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD01196.1"
                     /translation="MKGYLAFLKKEFFEYAKTYKLFIMLMVFAVFGITNPLIAKLMPE
                     IVKSFITDGVVITLPEPSAYDAWTQFFKNATQMGLIVMVILFSGVLASELSKGTLINL
                     L"
     gene            complement(306..1223)
                     /locus_tag="PLA01_07615"
     CDS             complement(306..1223)
                     /locus_tag="PLA01_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD01197.1"
                     /translation="MGVLEIRQLYKSFGHHEVLKGLDMDVPEHSIFGFVGQNGAGKTT
                     TMKIILGLLKPESGSVTVCGEKVTYGESKANRYIGFLPDVPEFYGYMNPMEYLKLCGE
                     IAGLTKTRIKSKSEELLELVGLKDQKKRIGGFSRGMKQRLGIAQALLNEPKLLICDEP
                     TSALDPIGRMEILDILLAAKGKTTIVFSTHILSDVERICDHIAVLHDGRLALCGTISE
                     IKERHRLHGFILEFESEEAASCLENAEELKQPSISLSRDGSTVKVFMKNGDMDGHFLL
                     GVLSQMRIVPLKFAVMEPTLESMFMEVVR"
     gene            complement(1223..1429)
                     /locus_tag="PLA01_07620"
     CDS             complement(1223..1429)
                     /locus_tag="PLA01_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HPD01198.1"
                     /translation="MDLATLKEYLPFLIPLVIAQFALAITALVHVLRHPRYRFGNKVM
                     WILIVLFIQFIGPALYFAFGRGDD"
     gene            complement(1488..2594)
                     /locus_tag="PLA01_07625"
     CDS             complement(1488..2594)
                     /locus_tag="PLA01_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPD01199.1"
                     /translation="MKEINIARTIVKMRREKGMTQEDLANYIGVSKASVSKWETGQSY
                     PDITFLPQLATLFNISIDELMGYEPQMSKEDIRKLYVKLSADFASKPFDEVLDSCREI
                     AKKYFSCFPLLFHIGALLVNNSMESGDKEKPLSVLSEAKELFVRVKTESEDAELVQLS
                     ICMEAYCALMMGNPNEVIELLEGTRTKIISNETLLASAYQMVGKSIEAKKILQVAIYQ
                     HMCNLFGALTDYLILCTDTPEQFEKTLKCANDIAEAFNLKKLHPSFLIKLYNVAAQGY
                     TMIGSKERALDILEKYTELVTGDIYPLQLKGDEYFNLIDQWIEELDLGNVLPRSEKNI
                     RKSIAGGVINNPVFAILADEIRFKRIAEKLKNIC"
     gene            complement(2796..3197)
                     /locus_tag="PLA01_07630"
     CDS             complement(2796..3197)
                     /locus_tag="PLA01_07630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPD01200.1"
                     /translation="MKLWLETNITAHDFIDEKYWRSKYEQVKEMMPKATIYVYEDDFI
                     KGFTGLSGNYVAGIFVEANSQSKGIGKALLDHIKGINKELFLHVYKKNERAVRFYIRE
                     GFFIKGEQTDNNTNETELVMKWINKKDCFYE"
BASE COUNT          942 a          745 c          540 g         1047 t
ORIGIN      
        1 cagcaaattg ataagggttc cttttgacaa ttcggatgct aaaaccccgc taaacagaat
       61 caccataact ataagtccca tttgcgtcgc atttttgaaa aactgtgtcc aggcatcata
      121 ggccgatggc tccggtaatg ttatgacgac accgtcggtt ataaagctct ttacaatttc
      181 aggcatcaat tttgctataa ggggattcgt gattccgaaa actgcaaaaa ccattagcat
      241 aatgaatagc ttataggttt ttgcgtattc aaagaactct tttttcaaaa atgccaaata
      301 gcctttcatc gaaccacctc cataaacata ctttccaaag tcggttccat cactgcgaat
      361 ttcaacggaa caatacgcat ttgcgatagc acacccaaca gaaaatgccc gtccatatcg
      421 ccatttttca taaacacctt tacggtgctt ccatcgcgtg acagactgat tgatggctgc
      481 tttaactctt ctgcattttc taaacaggaa gctgcttctt cactctcgaa ctcaaggata
      541 aaaccgtgga gcctatgacg ctcctttatt tcggaaattg taccgcaaag tgcaagcctt
      601 ccgtcatgta ataccgcaat atggtcgcat atcctttcaa catctgaaag aatatgagtc
      661 gaaaatacaa tagtcgtctt cccttttgcc gcaagcaaaa tatcaagtat ttccattctg
      721 ccaatggggt caagtgccga tgtaggttca tcacatatca ggagctttgg ttcattgagc
      781 aaggcctgtg caatgcccag gcgttgcttc attcctctgg aaaacccgcc aattctcttt
      841 ttttggtctt tcagtcccac aagctccagt aattcctcgc tctttgactt gattcgagtc
      901 ttcgtaaggc cggctatctc accgcacagc ttcaaatatt ccattggatt catgtaccca
      961 taaaactccg gaacgtccgg aagaaaacct atgtagcggt tggctttgga ttcgccatac
     1021 gtaacttttt caccgcatac ggttacagaa ccgctttccg gcttcaataa cccaagaata
     1081 attttcattg ttgttgtttt accagcaccg ttctgcccga caaagccgaa tatagaatgc
     1141 tcggggacat ccatatccaa tcccttcaac acctcatggt gcccaaagct cttgtaaagc
     1201 tgtctaattt ccaagacacc cattaatcat cacctctacc gaatgcaaag tataaggccg
     1261 gaccgataaa ctgtatgaac aagacgatca gaatccacat cactttattt ccaaaacgat
     1321 aacggggatg acgcaacaca tgtacaagtg cagtaatagc aagtgcaaac tgtgcgatca
     1381 ccaacggaat aagaaacggt aaatattctt tcaacgtagc caaatccatg attcactcct
     1441 ccttaaatga tgcattgatt tcatttcttt catgatgtgt ccggttgcta acagatattt
     1501 ttcagttttt ctgcaatcct cttaaatcga atttcatcag ctaatattgc aaacacaggg
     1561 ttattgatga ctccgccggc tatgctcttt cgtatatttt tttcactcct tggaagaaca
     1621 ttccctaaat cgagctcttc aatccactga tcaatcagat taaaatattc gtctcctttt
     1681 aactgcaatg gataaatatc accggtgaca agctctgtat atttttcaag aatatccagg
     1741 gctctttctt tacttccaat catcgtgtat ccctgggcag caacattgta gagctttata
     1801 agaaacgacg gatgaagttt ttttaagtta aaagcttcag caatgtcatt tgcacacttg
     1861 agcgtttttt caaactgttc gggggtgtct gtacaaagaa taagataatc cgttaatgct
     1921 ccaaagagat tacacatatg ctgatatata gcaacttgca atatcttttt tgcttctatc
     1981 gattttccaa ccatttgata ggctgaagca agaagcgttt cattggaaat gatctttgtc
     2041 cttgttccct ccaaaagctc aattacttcg tttggatttc ccatcatcaa cgcacagtat
     2101 gcctccatac aaatggaaag ttgcacaagc tccgcatctt cactttctgt ttttacccga
     2161 acaaacagct ctttggcttc cgaaagcaca gaaaggggtt tttccttgtc tcccgattcc
     2221 atgctgttgt taacaagcag tgctccaatg tggaataaca aaggaaagca ggagaaatac
     2281 ttttttgcaa tttcacggca agaatccaat acttcatcaa aaggtttgga agcaaaatcg
     2341 gcagataatt tcacatacag tttacggata tcctctttac tcatttgagg ttcataaccc
     2401 atgagttcat caatgcttat attaaaaagt gttgcaagct gcggcagaaa agtaatgtca
     2461 ggataactct gaccggtttc ccattttgaa accgaagcct tagacacgcc aatatagttt
     2521 gcaaggtcct cctgtgtcat ccccttctca cgccgcattt taacgatggt tctggctata
     2581 ttgatttctt tcatcttcat cacccacttt tattataccc tccaatcaaa tcatacctca
     2641 atggatttat ggttaatata gggtgataaa atcaactgta gggaaacttt cttatgaaca
     2701 aacctttcag tattataaga agagagtatc acttgctgct ttaggtagca atttgcaata
     2761 ctctcttttt tcagtatata atgctttaaa gggttttact catagaagca atctttctta
     2821 tttatccatt tcattacaag ttcagtttca tttgtattat tgtcagtttg ttcacccttg
     2881 ataaaaaacc cttctcttat ataaaatcta actgctcttt cattcttttt ataaacatgt
     2941 aaaaagagtt ctttatttat tcccttgatg tgatctaata aagcctttcc gattcctttt
     3001 gattgactgt ttgcttcaac aaatattcct gctacatagt ttccgctcag cccggtaaat
     3061 cctttaatga agtcatcttc ataaacataa attgttgcct ttggcatcat ttcttttact
     3121 tgttcatatt tactccgcca atatttttca tcaataaaat catgtgcagt aatattggtc
     3181 tcaagccata atttcattat actgtttaag tcagtttctg tgaactacca ccgcctatag
     3241 aggcagatgg cttcttggtc aatatctcta ttga
//
LOCUS       DAOOJC010000273         3270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_22682_length_3270_cov_3.069051, whole genome shotgun sequence.
ACCESSION   DAOOJC010000273 DAOOJC010000000
VERSION     DAOOJC010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22682_length_3270_cov_3.069051"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1807)
                     /locus_tag="PLA01_07635"
     CDS             complement(<1..1807)
                     /locus_tag="PLA01_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HPD01201.1"
                     /translation="MQIIVTPEELTNLAEIAKGISKRLDDVANEVKRIINSLDWELRS
                     KGALEERAQMAQTLALNMADGFTKMSQDLINAREQFIEADTKAASTSGNMKAATVKSS
                     SASELIAASIAPVMSASYSLWHGLFGPGTENCPNTFAGDPVNVVSGNFYLTRRDITIP
                     SRGMVLEITRYYNSMDNTRGIFGKGWRIDYETCLKKKEDSEDKIVVYPEGSIRVFEHT
                     EGGSYKSPKGEYDTLSKTENNTYILKRQKGFTYKYDQAGNLISISDTNNNEIEFKYNP
                     EGFLSSVVSPGGKFLTFTYEDDRVSCITDHTDRKIKYCYDEKGNLTQVVYPDEGKITY
                     AYDNIGLISITDQNDNTYVQNTYDKNGRVIKQLDHEKNELIIEYSEEKRENTFKWVKS
                     NITHVYKYNSEMILTEIKYDDGSVQKYTYDKNHNRNSETDRNGNTTYKKYDEKGNLIE
                     VISPKPFRLSTKYKYNENNKLIKAISPGDGEVSFEYDEKGNLLKRIIKTGSKSCSQWS
                     YTYDQYGRIITSTDAENNTTSFEYGEEDVNKPALVKDAAGNITKYKFDKAGRVTRVTT
                     GYGIVKMKYDDCDRITHITDAEGHTTILSYDKSGNM"
     gene            complement(1931..2566)
                     /locus_tag="PLA01_07640"
     CDS             complement(1931..2566)
                     /locus_tag="PLA01_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01202.1"
                     /translation="MKGLNSGLLRYNLKLIPKAGLIVSALLLLAIQAIFSLEIVDFLD
                     MARIGETYISILGIILIPGAVFIDENADIKEIIHSKSVSAVIPSIIRLIYLVTLLFFG
                     IVIFVVAAVVQGGNFNQGSIIAGVFISALLLGATGYLVGTFSKNMALSYLITFGYYGL
                     ELFTRGKYTKGFYLFSLQNGHLQRGKWVLFAIALLFFVISLTYMGNKKTCI"
     gene            complement(2550..>3270)
                     /locus_tag="PLA01_07645"
     CDS             complement(2550..>3270)
                     /locus_tag="PLA01_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD01203.1"
                     /translation="DEKLGGRTVITKERRISWMPYQPKSHEKCVEDKERLVELGFTSL
                     MAKMFSDYYGISLSMLTVFLSAFIFYRDRRSRILELICSRPVGGVTYVFSKFIGMCIP
                     IFASIMAVACIPTFYAIKMKLEGYNVNIFAYFSTFAVWLFPGVMFVVALSMMVSLLSN
                     SPIAAIGVQYIIFFLTVMPLYGDYSLSKVFIRHNTLEPLMNPQAFYINRIFIFVMSIA
                     ITFITAGIWQRKRRRIGERIK"
BASE COUNT          903 a          696 c          534 g         1137 t
ORIGIN      
        1 tcatattccc ggatttgtca tagcttagta tggtggtgtg accttcggcg tcagttatgt
       61 gggttatcct gtcacaatca tcgtatttca tcttcactat accgtagcct gtggttactc
      121 ttgtaacccg tcctgctttg tcaaatttat acttggttat atttccggcg gcatccttta
      181 ccaatgccgg tttattgacg tcttcctctc cgtattcaaa gcttgttgta ttgttttctg
      241 cgtccgttga ggttattatt cttccatact ggtcataggt atatgaccac tgtgaacagc
      301 tcttgcttcc ggtctttatg atacgtttta aaagatttcc cttttcatca tattcaaagc
      361 ttacctctcc gtcacccggg gaaattgctt ttatcagttt attgttctca ttatatttgt
      421 attttgttga gagtctaaaa ggctttggtg aaattacttc tattaaattg cccttctcat
      481 catatttttt atatgtcgtg ttgccgtttc tgtcggtttc gctgtttctg ttatgatttt
      541 tatcataggt atatttctgc acacttccgt catcatattt tatttctgta aggatcattt
      601 ccgaattgta tttgtataca tgggttatat tgctttttac ccatttgaag gtgttttcac
      661 gcttctcttc actatattct ataatcagct catttttctc atggtcaagc tgctttatta
      721 ctcttccgtt tttgtcatag gtgttttgga cataggtatt gtcgttctga tccgttattg
      781 aaataagtcc tatgttgtca taggcatatg ttattttccc ttcgtcagga tatactacct
      841 gtgttagatt tcctttttcg tcatagcagt attttatttt tctgtctgta tggtcggtta
      901 tgcagctaac tctatcgtct tcataggtaa acgtcaaaaa ctttccgccc ggtgatacta
      961 cggaagacag aaatccctca ggattgtatt taaactctat ttcattattg tttgtatctg
     1021 aaatggatat tagattaccg gcttgatcat atttatatgt aaatcccttt tgacgtttta
     1081 atatgtatgt attattttct gtttttgaaa gagtatcata ttcacctttg ggggatttgt
     1141 aacttccgcc ttccgtatgt tcaaataccc ttatgcttcc ttccggatat accactattt
     1201 tgtcttcgct gtcttccttc tttttgaggc atgtttcata atctattctc cagccttttc
     1261 cgaatatgcc ccgggtatta tccatggagt tgtaatatct cgttatctca agaaccatgc
     1321 cccttgaggg tatggttata tctctccttg ttaaataaaa gtttcccgat accacattta
     1381 cagggtctcc tgcaaatgtg ttcgggcaat tttccgttcc ggggccaaag agtccgtgcc
     1441 acaatgaata ggatgcagac ataactggtg cgatgctggc agcaattaat tctgaggcac
     1501 ttgaactttt aacggttgca gccttcatat ttccagatgt ggatgcagcc ttcgtgtccg
     1561 cttctataaa ttgttctctt gcatttatca ggtcttgaga cattttagta aaaccatctg
     1621 ccatgtttag tgccaatgtt tgagccattt gtgctctttc ttccaatgcc cctttgcttc
     1681 taagctccca atcaagggaa ttaattattc tttttacttc attagctaca tcatcaagtc
     1741 gtttcgatat tcctttcgct atttcagcca gatttgtcag ctcttccggt gttactatta
     1801 tctgcatatc caacctcagc cccttattta tataaacaat aaaatattaa ttatggaaat
     1861 tataccatta gctactttgg aattcaatta aatctctgta aaattgcaca ttaatacttt
     1921 ttatgattaa tcatatacag gtttttttat tccccatata agttaaacta attacaaaaa
     1981 acaataatgc aattgcaaat aaaacccatt ttcccctttg aagatgtccg ttttgcagac
     2041 taaaaagata aaatcctttt gtgtactttc cccttgtaaa caattcaagt ccataataac
     2101 caaaggttat cagatacgac agtgccatat tctttgaaaa ggttcccacc aaatatcccg
     2161 ttgctcccaa taacaatgca gaaataaata ctcctgcaat tatcgaccct tggttaaaat
     2221 tgcctccttg caccaccgca gccactacaa aaattacaat accgaaaaac aacagcgtta
     2281 ctaaataaat cagccttatt attgaaggaa taaccgcaga tacggatttt gaatgaatta
     2341 tttcttttat atccgcgttt tcatcaataa acacagcacc cggaatcaat attatgccca
     2401 agatggaaat ataagtttct cctatccttg ccatatccaa aaaatccact atttccaagc
     2461 taaagatagc ttgaattgcc aacagcagta aagcagagac tataaggcca gctttaggta
     2521 tgagttttaa attatatcgg agcaatccgc tatttaatcc tttcaccgat acgcctcctt
     2581 ttcctctgcc atatcccggc agtaataaaa gtgattgcta ttgacataac aaatataaaa
     2641 atcctgttta tataaaatgc ctgagggttc ataagcggtt ctaacgtatt gtgccgtata
     2701 aatactttgc tcagactgta atcaccatac agaggcataa ccgttaagaa gaaaattata
     2761 tactgtacac caatcgctgc tatggggctg ttactcaaga gggaaaccat catgcttaag
     2821 gctacaacaa acattacccc gggaaaaagc cacacggcaa atgtggaaaa ataggcaaag
     2881 atatttacat tatatccttc cagcttcatt ttaattgcat aaaaagtcgg aatacaggca
     2941 acagccatta tgcttgcgaa tataggtata cacattccga taaactttga aaacacataa
     3001 gtaacacccc caactggacg gctgcatata agttccaaaa tacggctgcg tctgtcacgg
     3061 taaaatataa aagcagaaag gaacaccgtc agcattgaaa gtgaaatacc gtagtaatcc
     3121 gaaaacatct tggccataag ggaagtaaat ccaagttcga ccaaccgctc tttatcttca
     3181 acacatttct catgagattt gggctgatac ggcatccagc taatgcggcg ttccttggta
     3241 attacagtac gtcctcccag cttctcatcc
//
LOCUS       DAOOJC010000274         3259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_22787_length_3259_cov_2.802434, whole genome shotgun sequence.
ACCESSION   DAOOJC010000274 DAOOJC010000000
VERSION     DAOOJC010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22787_length_3259_cov_2.802434"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLA01_07650"
     CDS             complement(<1..299)
                     /locus_tag="PLA01_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPD01204.1"
                     /translation="MISSDVIRGYNDTIILSLLIKGDSYGYEISRNIRTITNEQYVMK
                     ETTLYSAFARLEKNGYIESYYGEETQGKRRTYYRITARGIEYYKEKCEEWNLTKNV"
     gene            complement(561..1382)
                     /locus_tag="PLA01_07655"
     CDS             complement(561..1382)
                     /locus_tag="PLA01_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPD01205.1"
                     /translation="MLYLFKRISIFVLIFMFVFLVAQVGAYAYSSENVIDNLGYLTET
                     EVQYLQSSIDRIKENYRLDTVIVITDDTAGKSSRDFADDFFDNNGYGIGSDYSGLLLL
                     INMNKRDVYISTSGKAFDIFTDARIDNILDDVARFLSNGNYYAACSEFISNVKSYAYS
                     GVPSNQYRVDTDRNYFHNVLSMMKFFPIYIVALIISVIATLVVTYSSKGKTTTNHLTY
                     EVNGSFDLTENQNNYIRESTVRTRIESSSSGGGSGRSSTHRSSSGRTHGGGGRKF"
     gene            complement(1366..2394)
                     /locus_tag="PLA01_07660"
     CDS             complement(1366..2394)
                     /locus_tag="PLA01_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PriA"
                     /protein_id="HPD01206.1"
                     /translation="MSSVKEYKCPSCGASLQFDPPTQSWKCHYCSTEYSKDQFDKQSQ
                     EELPDEDMPELDSYICNSCGAELIADETTSATFCLYCKSAAIIKSRFSGRFKPKSVIP
                     FKLTKEQSKQIYSKWIHKRILAPKEFKQKEEIDKVTGIYAPFWLFNCRSNGFIQGEAR
                     TVHSWRSGNYRYTQTKYYNFLRRGNSSYNNIPVDASKKLDDKFMQMIEPYDYNEIQNF
                     SMKYMSGFMAERYDVEAAQAETVMRNRVEDYFEKRLRSTVTSYSSYTVNSKQVVLSDI
                     ENSYSMLPVYLLINKYRDKDHIFIINGQTGKVVGDTPISPFRQIVFGVVVFLIVWIIA
                     VFGGALFV"
     gene            2890..>3259
                     /locus_tag="PLA01_07665"
     CDS             2890..>3259
                     /locus_tag="PLA01_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPD01207.1"
                     /translation="MKMKVGFQIKSFFSKISLKNISISIKLNVIISVIMILSLSVSIV
                     YSFNVVTSILLDQVKETTLQVTRQTSLNMRMLLEELDMLAMDLSRDEHLAELISSIDN
                     AQDEQEKGRYDSQLKTALTDY"
BASE COUNT          971 a          589 c          607 g         1092 t
ORIGIN      
        1 acattcttag tcaagttcca ttcttcgcat ttttccttgt agtattcgat tcctctcgct
       61 gttattctgt aataggtccg tctttttccc tgagtttctt caccgtaata ggattcaata
      121 tatccgtttt tttcaagacg tgcaaatgca gaataaagtg tggtttcctt cataacatac
      181 tgttcgtttg ttattgtgcg tatattcctc gaaatctcat acccatagga atcgcctttt
      241 ataaggaggc ttagaataat ggtgtcgtta tagccccgta ttacatcact gctgatcact
      301 aagatccctc ctttaacaac tactacgtct gacgtacttt acctgtcgta gtaattataa
      361 cataactact acgacaggta aagtactttt ataaaaaaat ttttatttat aataaaaatg
      421 gaaaagtaaa aagtcaagaa agcaagatat ctgccgttct tgacgcaaat agacatgtct
      481 ttggattttt attaagaacc ggttttatat tcttaatgga aattgtattt aataagctaa
      541 agacttgtta attacaacca ctagaacttg cgtccgccgc caccatgagt tcttccgctg
      601 ctgcttctgt gagtgctgct tctgccgctg cctccgcctg atgaactgct ttcgattctg
      661 gttcttaccg ttgattccct tatatagtta ttttggtttt cagtgagatc gaaagagccg
      721 tttacttcat aggtaagatg attggttgta gttttgcctt tactggaata tgttacaacc
      781 agagtggcaa taacagaaat aataagggcg acgatatata tgggaaagaa tttcatcatt
      841 gataaaacgt tatgaaagta gtttctatct gtatcgactc tgtattgatt ggatgggaca
      901 ccggaatatg cataactttt cacattgctg ataaattcac tgcaagccgc atagtaatta
      961 ccgttggaaa ggaatctggc cacatcgtct aatatattgt caattcttgc atctgtaaaa
     1021 atatcaaagg ctttgccgct tgtggaaatg taaacatccc ttttattcat gttaataagc
     1081 agcaaaagtc cggaataatc acttccgatt ccgtagccgt tgttatcgaa aaagtcatcg
     1141 gcaaaatcac gggaggactt gcccgctgta tcatcggtaa taactataac ggtatcaagc
     1201 ctatagttct cttttattct gtcaatactg ctttgaaggt attgaacttc ggtttctgta
     1261 aggtaaccta aattgtctat gacattttcg gaactgtatg cgtaggctcc tacctgtgca
     1321 accagaaaaa caaacatgaa aatcagcaca aaaatactta tccttttaaa caaataaagc
     1381 acccccaaat acagcgataa tccaaacaat taaaaataca accaccccaa aaactatttg
     1441 cctaaaaggg cttatgggag tgtcacccac gaccttgccg gtctgaccgt ttattatgaa
     1501 tatgtggtct ttgtctctgt atttattgat aaggagataa acgggcagca tggaatatga
     1561 attttcgata tccgacagca caacctgttt actgttaacg gtataagaag aataactggt
     1621 tacagtactt ctcaaccttt tttcaaaata gtcttcgacc ctgtttctca taacagtttc
     1681 cgcttgagcg gcttcaacat cgtatctttc agccataaaa ccggacatat acttcattga
     1741 aaagttttga atttcgttgt agtcataagg ctctatcatt tgcatgaatt tatcatccag
     1801 tttcttggaa gcatccactg gaatgttatt gtagctggaa ttaccccttc gcaagaaatt
     1861 ataatactta gtctgggtat atctgtaatt gccgcttctc catgaatgta cggttcttgc
     1921 ttctccctgg ataaagccgt tggatctgca gttaaagagc cagaaaggtg catatattcc
     1981 ggttacttta tcgatttcct ctttttgttt gaactctttg ggagcaagga ttcgtttgtg
     2041 aatccatttg ctataaatct gtttggactg ctcctttgtc aatttaaagg gtataacgct
     2101 ttttggctta aaacggcccg aaaaccttga ttttatgatt gcggcacttt tgcaatacag
     2161 gcagaaagta gcggaagtcg tctcatctgc tatcaattcg gctccacaac tgttacaaat
     2221 gtatgagtcc agttccggca tatcctcatc gggaagctct tcctgactct gtttatcgaa
     2281 ctggtctttg gagtattcag ttgagcaata gtggcatttc caactctgag taggcgggtc
     2341 aaattgtaac gatgcaccgc aagagggaca tttatactct tttaccgagc tcatttattt
     2401 tctcctttaa caagtagtta atttcaatgt ttaccccttc caaaatgtac aaaaaatgaa
     2461 tctataaggt tggagggttc aactatttaa gacatatttt aattatacat tcacaaaaat
     2521 atgctgtcaa actgtagttt tacttcactt ttctttaagg ccgtcaaacg ctgatacgac
     2581 tagaaggatg tataaagaca aaacagtgtc aaataggtaa ttattgtctt taacatgtgt
     2641 aattatatat gatataataa acttaagaag aaatatgaaa ataactgttt attatcagac
     2701 attcggtatc aggtttgcac gatatttttt atgtatcctt tcttgtgcaa tacagtaatt
     2761 caaaagtaat aaatttttat gtttatcgtg gctttcaaaa tggcgatata tatagagaga
     2821 gcttgatttt tttatgttat tgaacctcta attagctgtt tgtatgcttt taatttataa
     2881 gaggtaggtg tgaagatgaa ggtggggttt caaataaaaa gtttttttag taaaatcagt
     2941 ttgaagaata taagtataag tattaagctt aatgttatta ttagtgtgat aatgatattg
     3001 tcattatccg tttcaatagt atattctttt aatgttgtta cgagtattct gcttgatcag
     3061 gtcaaggaaa ccacattgca ggtaaccagg cagacttcct tgaacatgcg gatgctgctt
     3121 gaggagctag acatgttggc tatggattta agcagggacg agcatttggc agagttgata
     3181 tcaagtatag ataatgcaca ggatgaacag gaaaagggca ggtatgacag tcagcttaag
     3241 acggcattaa cagactatg
//
LOCUS       DAOOJC010000275         3252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_22842_length_3252_cov_2.738192, whole genome shotgun sequence.
ACCESSION   DAOOJC010000275 DAOOJC010000000
VERSION     DAOOJC010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22842_length_3252_cov_2.738192"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PLA01_07670"
     CDS             <1..492
                     /locus_tag="PLA01_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01208.1"
                     /translation="GGMSASRAEAFVNIKELKENTEEIVRLKIISTETKHHEGLNFTL
                     SEARVLKVINGNDIAEGDTIKLIQTGAIIDGIENSIFGDPIYRVDDEMILFLKKSKSS
                     FAENVYRSIGISDGRFDIIDSKVVARGLGYSESEGSDFEEMSFDDINELRLTVGEFEE
                     RIR"
     gene            499..1317
                     /locus_tag="PLA01_07675"
     CDS             499..1317
                     /locus_tag="PLA01_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HPD01209.1"
                     /translation="MFFNYVLFPTVITGIILFIIGQRVSRVVNRKPVRILLGFVFVLL
                     CVPNVLFAAYYLHILNEPSWYVKFRAINNIELLSSFIGLFFGFITFKDESFGKIKLKT
                     LNKYMFVICILLITIPFIKPIIRPVSRNFEFSNRWRDGVCLQSTGSTCGPAALATILN
                     YYGISKSEEEIARASFSSSSGTENWYLIRYAEKNGLETEVLYKDSLENVPAPSIIGTY
                     IRGGIGHFITVLGKEGDRFVVGDSLKGRLMLTEKEFNEHYTFSNFVLYIKKPEG"
     gene            complement(1602..3170)
                     /locus_tag="PLA01_07680"
     CDS             complement(1602..3170)
                     /locus_tag="PLA01_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HPD01210.1"
                     /translation="MRVSKALQRGVLVWVFFLLIISCIAVSTFTASAGRLGDINSDGE
                     IDSTDVTLLKRHLLRKNILTGTAYSNADTDGDRDVTSTDLTFLKRYVLHRISSFPGET
                     ANPTPSPTPSPSINLNIPWDWTGIIGTGQSLSVGATPILSTTQPYNNLKLSLGNLSVP
                     PYNANSSELKMVPLTEPIRSLAMGFPAAYPRNLYGETPHTAMANQITEMVKGAGGNDY
                     ISVHTVVGESGQGMSVIKKGATDKGNSGRSYAASVFEVTAINRLAKASGKTYGVGGIT
                     LIHGETDCGNPYYENEMRQMWSDYNQDIKAITGQTQNIPMFVVQQHSYPSTGTSASTL
                     AQWKAGVDYPDDIICIGPNYQRYFGGDNVHLTSTGYQHLGEKFAQVYYERLVLGNDWK
                     PLQPIKATKNGRTITVDFHVPVPPLVWDNTLPAPNQSTLTEWRNGKGFEVTANGSRVT
                     INSVEISGNSVIITCANDLPAWGVKVGYAFTGGTARTNGTYRWGLLRDSDPFKGRTGL
                     AQPNFCVAFEMSVN"
BASE COUNT          926 a          586 c          752 g          988 t
ORIGIN      
        1 gggggcatga gtgccagtag agcagaggcg tttgtcaata taaaggaatt aaaggaaaat
       61 acagaagaga ttgtacgttt aaaaatcata tcaaccgaaa ccaagcatca tgagggtttg
      121 aattttactt tgtctgaggc tcgggtattg aaggttatca atggtaacga cattgcagaa
      181 ggagatacaa taaaacttat acaaaccggg gcaattatag acggtataga gaacagtata
      241 tttggagatc cgatttatag agtggatgat gaaatgattc tgtttctcaa gaaaagcaaa
      301 tcaagttttg ctgaaaatgt ctatcgaagt ataggcatta gtgacggaag atttgatata
      361 atagattcta aagtggtagc aagaggtttg ggctacagtg agagcgaagg ctcggatttc
      421 gaggaaatga gctttgacga tataaatgag ctgaggctta ctgtaggaga atttgaagag
      481 agaataaggt gagttgaaat gttttttaac tatgtactat ttccgaccgt aataacgggc
      541 attatacttt ttattatcgg gcaaagggtt tcaagggtag tgaaccgaaa acccgtaagg
      601 attcttttgg gttttgtatt cgtccttctt tgtgttccga acgttctgtt tgcggcatat
      661 tatttacata ttttaaacga gccttcatgg tatgttaaat tcagggctat taacaatatt
      721 gaattgttat cgtccttcat aggtcttttc tttggattta taactttcaa agatgaaagc
      781 ttcggaaaga ttaaattaaa aactttaaat aaatatatgt tcgttatctg catacttttg
      841 attacaatac ctttcatcaa accgattata agacctgtaa gccggaattt cgagttttca
      901 aaccgatgga gggatggggt gtgtctgcaa tctacaggtt caacatgcgg tcctgcagct
      961 ttagcaacaa ttcttaatta ttatgggata tcaaaaagtg aagaagagat agcaagggcg
     1021 tccttttcca gttcaagcgg cacggagaat tggtatttaa tcaggtatgc cgaaaagaac
     1081 ggccttgaaa ccgaagtttt atacaaagac agtttggaaa atgtaccggc accgtctata
     1141 atcggaactt atattagagg cggaataggt cattttataa cggttttagg caaggaagga
     1201 gaccgctttg ttgttggaga ttcgttaaaa ggaagattaa tgttaaccga aaaggagttt
     1261 aatgaacact atactttttc aaattttgtt ttgtatataa agaagccgga aggctaacct
     1321 gctttgtatg ttgttgatgt tatgccggag catgttattt aagtgaagaa aaacatggaa
     1381 ggaactttgt gaagtaagtt atagagcctg ttaatactat tgcgggaaag ggagcatcca
     1441 ttctgacgaa gtgggttttg ctcagcccgg cggagtattt acaggctctg tacttatgtt
     1501 ttaaacatta ttcttcaaat ttagtcggat tttacggggt gaaatcacta aaaaagtgaa
     1561 tgggttcgat gccctcaagt tattgtttat ttaatgctcc tttaattaac tgacatttca
     1621 aaagcaacac agaaattagg ctgtgccaaa ccggtccttc ctttaaaagg atccgagtca
     1681 cgcagtaaac cccagcggta cgttccatta gttcttgcag ttccgcctgt aaaggcatag
     1741 ccgactttta caccccaagc tggtaggtca ttggcacagg ttattattac tgaatttcca
     1801 gagatttcaa ccgaatttat tgtgactctt gaaccgttgg cagtgacttc gaagccttta
     1861 ccgtttctcc attcggttaa ggtgctctgg tttggtgccg ggagtgtatt atcccaaacg
     1921 aggggcggta ccggtacatg gaaatctact gtgatggttc tgccgttttt ggtagctttt
     1981 ataggctgaa ggggtttcca gtcgttaccg aggacaagcc tttcgtaata gacctgagcg
     2041 aacttttcac ccagatgctg atatccagtg gaagtcaaat ggacgttatc gcctccgaaa
     2101 taacgttgat aattgggacc tatacagatt atatcatcgg gataatcgac acctgctttc
     2161 cattgagcca aggtagaagc ggaagtccca gtacttggat aggagtgctg ctgcacaaca
     2221 aacatcggga tattctgggt ttgtccggtt atagccttta tatcctgatt gtaatccgac
     2281 cacatttgac gcatctcatt ttcatagtaa ggattgccgc aatcggtttc tccatggatg
     2341 agggtgatgc ctcccacacc gtaggttttt cccgaggctt ttgccaagcg gttgattgca
     2401 gttacttcaa atactgatgc cgcataagaa cggccggaat tacccttatc agtggcacct
     2461 tttttgatta cagacatacc ctgtccggat tcaccgacta ccgtatgtac ggaaatatag
     2521 tcgtttccgc ctgctccttt caccatttca gttatttggt ttgccattgc agtatgggga
     2581 gtctcaccat ataggttcct tgggtacgca gccgggaaac ccattgcaag actgcgaata
     2641 ggttcggtaa ggggaaccat tttcagttca ctgctgttgg cattgtaggg aggaacgctt
     2701 aagttgccca aagacagctt cagattgttg taaggctggg ttgtagataa aattggagta
     2761 gctcctacgg agagactttg acctgtgcct atgattccgg tccaatccca gggtatgttg
     2821 agattaattg acggtgacgg ggtcggcgat ggtgtcggat ttgcagtttc acccgggaaa
     2881 gaagatatgc ggtgcaatac ataccttttt agaaaggtta agtcggtgga ggttacatcc
     2941 ctgtctccat cggtatcggc attggagtaa gccgttcctg ttagaatgtt ttttcttaaa
     3001 agatgtctct ttaataatgt cacatcggta gaatcgattt ccccgtcact gtttatatct
     3061 cccagtcttc cggcactggc agtgaacgtt gatactgcaa tgcatgaaat aatgagtaaa
     3121 aaaaataccc aaaccaaaac acccctttgt aaagcttttg aaaccctcat aatttatccc
     3181 ccttctttta aataatactt agttgtttgt aaaatgctgt aacccttgaa accaaagggt
     3241 tttagcagta ta
//
LOCUS       DAOOJC010000276         3247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_22874_length_3247_cov_3.323308, whole genome shotgun sequence.
ACCESSION   DAOOJC010000276 DAOOJC010000000
VERSION     DAOOJC010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22874_length_3247_cov_3.323308"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(922..>3247)
                     /locus_tag="PLA01_07685"
     CDS             complement(922..>3247)
                     /locus_tag="PLA01_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HPD01211.1"
                     /translation="SGNTTKFEYDEAGRLGKVTDALGNSTSYEYDDFGNMISQTDANG
                     NKTTFEYDNFGRKIKRTLPLGMSETYTYDAVGNIVAMTDFNGNTTKFEYDVNDRLTKK
                     IYPDGSEETYTYTVSGKRETVTDSRGTTTFKYDVMGRLIKETGPDGISIEYTYDAAGN
                     RTTVKVPSGTTTYTYDKLGRLSAVTDPDGGVTTYTYDEVGNRTGVSYPNGTKTVYTYD
                     KLNRLVRLVNQGADGEIISSYEYTLDPSGNRIKIEENSGRVIEYEYDNTYKLLKEAIK
                     NPDQSTREISYTYDAVGNRLTKTDGEYTVNYSYDKNNRLISEGENIYTYDNNGNTTSK
                     KNTKETISYTYGYNNRLISVVTTNQEGTSTVEYIYDTDGIRVGKIVDGTKVSRYTVDK
                     NTDYAKVLEERDENGALVVSYVHGDDLISQKRGNVKSYYQYDGLGSTRALTDNSGEIT
                     DTYTYDAFGNLIEKTGNTENEFLYTGEQYDANIGFYYLRARYLDPTIGRFVTMDSFEG
                     IVKDPYSLHKYLYAHANPVMNTDPSGLFTLGQQVAVLAISTILANSLITCISLFNTVR
                     GVGDDAPIDGFIVNARGGAFLRGFTVGGGLDIIIDIVNSDILVSFALEGGTDPLSLFK
                     NHRKGGLGITAGPIFNMSNPQQMADFGFSAAWPLSVLHLIGRIMTFRGSTWGLMTQFA
                     KNAKNNRGWTAVFGQSTSGPAYFAIGPSSRSFSSLIGWDSQYVSLKELSPQIGNEMYN
                     WINDISHSIGKWTSFKDLCESADSFMSIISSYSM"
BASE COUNT          940 a          690 c          481 g         1136 t
ORIGIN      
        1 ttgtcttgcc aactataatt atactctaag tttcacatag aacaggggac ggttttttgt
       61 tccactgcaa tggctattaa taatggaaaa cctccgctgt aagtatttac aacggaggtt
      121 ttttgtcatt tcgggaatct aatcgcgttt tctcgttccc gcaagacctg ccatatagct
      181 attaaacata ctgcggattt ggattaccct gtcctattta tcgtaaacat actatgcagc
      241 tcttcatgaa gtggacacgg tacctgttcc tcctgtctct taactgtatc ttctaaattc
      301 tactcatcga acattaccaa atgttctttt tttataatat caataaatct ccaatattca
      361 atttgtatat ttttatccgc aattgcttga aaaagtgaac ctgacgtagt tatttaatac
      421 cgtacattat aatacattaa ggtcttcaga gaattgataa aacactataa caaaacaata
      481 caaccaccaa gctaaataca attagataaa taattcttat aattacttcc gctcttgttt
      541 ttaattcttt cttttttatc ttatagcaca attctcttat ataggtaaaa tacaaaaaga
      601 caaaacaccc taaaagcgaa aaaatgaata aattataaaa aactttattg gggaaaatca
      661 ggaacataat aagggataca ataattacca atacacaagc aactacttct ttactcattc
      721 ttgagtataa ttttgattta ttcataatcc ctaactcctt ttacggttaa ttttttataa
      781 ttctcatttt gctgctgctt gtcgtagttt caggatgcca tgtatcaacc tgctctcgaa
      841 accttcatcc attagagtat acttatgaca gtagaatcac ataagataac cgccaatata
      901 aattggcggt tatcaatact cctacatgga ataactactg ataatactca taaaactatc
      961 tgcagattca caaagatcct taaagctagt ccacttgcct atggaatgtg aaatatcgtt
     1021 tatccaatta tacatttcat ttccaatttg aggtgataat tctttaagtg atacatattg
     1081 agagtcccaa cctatgagtg atgaaaaaga tcttgatgaa ggaccaattg caaaatatgc
     1141 cggtccggaa gttgattgtc cgaaaaccgc cgtccaacct ctattatttt ttgcattctt
     1201 agcaaattga gtcatcaatc cccacgtgct acccctaaat gtcattatcc ttcctataag
     1261 gtgtaaaaca gataaaggcc aagccgctga aaaaccaaaa tccgccatct gctgaggatt
     1321 tgacatgtta aatatcggac ctgcagtaat acctaatcca ccttttctat ggtttttaaa
     1381 aagtgacaaa ggatccgtcc caccttctaa tgcaaaagaa accaaaatat cactattgac
     1441 aatatcaatt ataatatcca atcctccgcc tacggtgaat cctcttagaa aagctcctcc
     1501 tcttgcatta acaataaatc cgtctatcgg tgcatcatca ccaacgcctc ttacagtgtt
     1561 aaataatgat atgcacgtta taagcgagtt tgctaatata gtagaaatag ccagaacagc
     1621 tacttgctgc cctaatgtaa acaaaccgct tggatccgta ttcattacag ggtttgcatg
     1681 agcatataag tatttgtgca aactataggg gtccttaaca attccttcaa acgaatccat
     1741 cgtaacaaat cttcctatag tcggatccaa gtaccttgct ctcagataat agaatcctat
     1801 attagcgtca tactgctctc ccgtgtacaa gaattcgttt tccgtatttc ctgtcttttc
     1861 aattaggttt ccaaaagcat catatgtata tgtatccgtt atctctccgc tgttatcggt
     1921 caatgccctt gtgcttccca gtccgtcata ttggtagtag ctcttaacat tacctctttt
     1981 ttgacttata aggtcgtctc cgtgtacata acttactacc aatgcaccat tttcatctct
     2041 ttcttccaaa acttttgcat aatccgtatt tttatctacg gtatatctgc tgacttttgt
     2101 gccgtcaact attttcccga ctcttattcc gtctgtatca taaatatatt ctacagttga
     2161 tgtaccttcc tgatttgttg taacaacact tataagccta ttattatatc cataggtata
     2221 tgatatggtc tctttagtgt ttttctttga agtagtgttt ccgttattgt cataagtata
     2281 gatattttcg ccttcagaaa taagcctgtt gtttttatcg tatgaataat ttacagtata
     2341 ttcaccgtct gtcttagtta gcctgtttcc cacagcatcg taggtatagc ttatttcacg
     2401 agtactttgg tcaggatttt ttatagcctc tttcaacagt ttataagtat tgtcatactc
     2461 atattcaata actcttccgc tgttttcttc aatctttatt ctattacctg atgggtctaa
     2521 tgtatactca tatgatgaaa ttatctcccc gtcggcaccc tggttaacaa gccttaccag
     2581 tctgttaagc ttgtcatatg tatataccgt ttttgttccg ttagggtaag aaacgccggt
     2641 tctgttgccc acttcatcat atgtatatgt tgttactcct ccatcagggt ctgtaactgc
     2701 ggaaagcctg cctaacttat cataagtata ggttgttgtt ccggatggaa ctttgacagt
     2761 ggttctgttt cctgccgcat cgtaagtata ttctatgctt attccatcag gaccggtctc
     2821 ttttatgagt cttcccataa catcatattt gaatgttgta gtacctctgc tgtctgtcac
     2881 tgtttctctt ttacctgaaa cagtatatgt atacgtctct tccgaaccat cgggataaat
     2941 cttcttagtc aacctgtcat ttacatcgta ttcaaatttt gtagtattac cgttaaaatc
     3001 agtcatagca actatgtttc caaccgcatc ataagtgtaa gtttcagaca ttcccaaggg
     3061 tagagtacgt tttatctttc tgccgaaatt gtcatattca aaagtagttt tatttccatt
     3121 ggcatccgtt tgggatatca tattcccaaa atcatcatat tcataggatg tcgagttccc
     3181 cagggcatct gtaactttac caagtcttcc cgcctcatca tattcaaact ttgttgtatt
     3241 tcctgac
//
LOCUS       DAOOJC010000277         3247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_22878_length_3247_cov_2.834273, whole genome shotgun sequence.
ACCESSION   DAOOJC010000277 DAOOJC010000000
VERSION     DAOOJC010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22878_length_3247_cov_2.834273"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..1078
                     /locus_tag="PLA01_07690"
     CDS             254..1078
                     /locus_tag="PLA01_07690"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPD01212.1"
                     /translation="MNRKGVTLIELIISISIVMIVAAPFLGTFLSAVKNNTASKEIME
                     SSTLALRVMEEIKSRPLFLSSEAVTQTQSPLTQYREYLNEEEYVVKYKIVKEEGEVSS
                     LSETYEFEKTEDTVFNIEFSIDSGTVILNGQSYQKYADGLPVLFSLEISEESGVYSYS
                     FYDEDNDGLQSSVLGTVPLTEPLKIKINCLNESSETFYMNVNLDGIEEDRDVYFYVFN
                     DRNNAVYLSNSGTKPFYRLLRIDGEQVGYFNSLYKIEVVIEKEDKIVNRLVSYVKK"
     gene            1088..>3247
                     /locus_tag="PLA01_07695"
     CDS             1088..>3247
                     /locus_tag="PLA01_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01213.1"
                     /translation="MIIRILSSNKGSTLITSILIIIMMSLLGISLMAVTLSSLDMSVF
                     YSDLNRAYFLAEAAAEEVAKTVDGMVGDIQESSRGQVSYMLQKQLVENPVSLRDPDGS
                     VSEGSFETEYRSRMEEEFKSLYFDYFYDELQNAFVDIGELDNLKMLLNTEHDDGFVAY
                     KDVAADYGRMVLESAFYDDTNKTLNIRVCGIYNDYKKRLEVSFGLLPEPDKPPFQVIE
                     KARVKNPVKYNILKKAVVAEKNLIITGGNVHIAGDVLSFGTVPFYEALSPGQKQEDFS
                     APWYSYGGIMVGMCDSVAERDDEFGFDSAKTGTFAKGSLSVDGNVATMGYIHTLYSSR
                     TDPSSISISGDTYSRSVRSEKYSNYSTMSFGNLSTIDNLQIDSNGSVIDINGVYKGFV
                     DTWRAIDGSSGMDSSEDEVVPKRTSSVVVNGDSLLNFKNAVYIGGSTFLKNLTDQQGN
                     PYMTGISALKSSEGVIKAFLKDDVSNPENRLFWFENGQYVEPFPEPYYKQYSQGENAF
                     DMLSGRASDPGYFPIINRAMHFKKVWEVFWKPDDIFFTNINPDNISITGNGIAEDGRI
                     NGFSNGAVIANGRVYGVDDFVDFDPSSFHINIQKPTIENYYDSVSGLLNESYNPEYPK
                     LNFVEPTKNVTDYIDGDFIGRNRVELDKPYIPMDVNKGLLYYGTKDVRIERAGVNWYI
                     DGNLIPVAKGIILTDGNIYIEDGFSFTGILLSTKNIVF"
BASE COUNT         1000 a          544 c          771 g          932 t
ORIGIN      
        1 ttaattaaca agacggacaa aaagctctcg gtagacgtaa tgaatgatga cattaataga
       61 cccaggataa gaataacgag atctaccggt aacatagagg tcaactacaa ataaatccgc
      121 tttctaataa caattctttt taacgtttta ccgactcctt gtaattttat gtaatacacc
      181 gtaaaatcag tgctttgaac tgctgtaaaa tgatttacag taaaaaaaac gtactgggat
      241 gggatggtgt gatatgaata gaaaaggtgt aactctaatt gagttgataa tttccatttc
      301 tattgtaatg attgttgccg caccgttttt aggtacattt ctttctgctg taaagaacaa
      361 tactgcatca aaggaaataa tggaatcctc aacgctggca ttgagggtaa tggaggaaat
      421 caaatcaagg cctctgtttt taagctccga ggctgtaacc cagacacagt caccgttgac
      481 acaatacagg gaatacctga acgaagagga atatgttgta aaatataaga tagtcaagga
      541 agagggagag gtatccagct taagcgaaac ctatgagttt gaaaaaacag aggatacggt
      601 gtttaatatt gagttttcca tagattcggg aacggttatt ctaaacggtc aatcctatca
      661 gaagtatgcc gatggtttgc cggtgctttt ttccttggaa ataagcgaag agtcgggggt
      721 ttactcttac agtttttacg atgaagacaa tgacggattg cagtcatcgg ttttaggcac
      781 tgtccctctg acggaaccat taaaaatcaa aataaattgt ttaaatgaaa gcagtgaaac
      841 tttttatatg aatgtcaatc ttgacggtat tgaagaagac agggatgttt atttttatgt
      901 ttttaatgac aggaacaatg ccgtttatct cagcaacagc ggaacaaaac ctttttaccg
      961 gttgcttcgg atagacggtg agcaagtcgg ttatttcaat tcgctgtata agattgaggt
     1021 tgttattgag aaagaggaca agatagtaaa caggcttgta tcttatgtaa agaagtaagg
     1081 tggttatatg ataatcagga tattaagttc aaataaaggt tcaacactga ttacttcaat
     1141 actgattatt ataatgatgt ctcttttagg tatatcttta atggcggtta ccttaagctc
     1201 actggacatg agcgtttttt acagtgattt gaacagggct tattttcttg ccgaggctgc
     1261 ggccgaagag gtggccaaaa ccgtcgatgg gatggtggga gatatccagg aaagttccag
     1321 gggacaggtg tcttatatgc ttcagaaaca gcttgtagag aatcctgtaa gcttgaggga
     1381 tccagacggt tcggtttcgg aaggttcttt tgaaaccgaa tatcgctcca ggatggaaga
     1441 agaattcaaa tcactgtact tcgattactt ttatgatgaa ttgcaaaatg cttttgttga
     1501 catcggtgag cttgataatc tgaagatgct tctgaatacc gaacatgatg acggttttgt
     1561 tgcatacaag gatgtcgcgg ctgattatgg cagaatggtg ctcgaatcgg ctttttatga
     1621 cgatacgaat aaaaccctta atataagagt ttgcggcata tacaatgatt acaaaaaaag
     1681 gttggaggtt tcctttggct tgcttccgga accggacaaa cctccgtttc aggtaattga
     1741 aaaggcaagg gtgaaaaatc ccgtgaaata caatatactg aaaaaagctg ttgttgcaga
     1801 gaaaaacctg attataacgg gaggaaacgt gcatatagcc ggggacgttt taagctttgg
     1861 aacggttccg ttctatgaag cactttctcc ggggcaaaaa caggaggact tcagtgcacc
     1921 ttggtacagt tatggcggca ttatggtggg aatgtgcgat agcgttgccg aaagggacga
     1981 tgaattcggg tttgattcgg cgaagaccgg gacttttgca aagggttctc tttccgttga
     2041 cgggaatgtg gcgaccatgg gatacataca taccctatac agttcgagaa cggatccttc
     2101 gagcatatcc atatcgggag acacttattc aaggtcggtc agatccgaaa agtattccaa
     2161 ttattccacc atgagtttcg gcaatctttc cactattgac aatcttcaga tagactcaaa
     2221 cggttccgta atcgatataa acggagtata taaaggcttt gttgacacat ggcgtgcgat
     2281 tgacggcagc tccgggatgg actcctctga ggatgaagtg gtaccgaaga ggacaagcag
     2341 tgtcgtagta aacggtgatt cgcttttgaa ttttaaaaat gctgtatata tcggcggaag
     2401 tacttttttg aagaatctga cggatcaaca gggaaatcct tatatgacag ggatatccgc
     2461 cttgaaatcc tccgagggtg ttataaaggc atttttaaaa gatgatgttt caaatcctga
     2521 aaacaggctg ttttggttcg agaacggaca atacgttgaa ccctttccgg agccttacta
     2581 taagcagtac agtcagggag agaacgcctt tgacatgctt tcaggaaggg catcggatcc
     2641 cggttatttt ccaatcataa accgtgccat gcattttaag aaggtatggg aggtgttttg
     2701 gaaaccggat gatatttttt ttacaaatat aaatccggac aatataagca ttacaggcaa
     2761 cggaattgct gaagacggca gaattaacgg tttttccaac ggagcggtta ttgccaacgg
     2821 cagggtgtac ggtgttgatg attttgtgga ttttgatccg tcaagttttc acattaatat
     2881 acaaaagcct accatagaaa actattatga cagtgtcagc ggacttttga atgaaagcta
     2941 taatcctgaa tatccgaaac tgaactttgt tgagccgact aaaaacgtaa ccgactatat
     3001 tgacggtgat tttataggcc gcaacagggt tgagttggac aaaccctata ttccgatgga
     3061 tgttaacaag gggttgttgt actatggcac caaagatgtg cggatagagc gggccggcgt
     3121 aaattggtac atagacggta atcttatacc agttgcaaag ggaataatac taacagacgg
     3181 aaatatttat attgaggacg gttttagttt tacggggatt ttattgtcga caaaaaatat
     3241 agttttt
//
LOCUS       DAOOJC010000278         3224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_23081_length_3224_cov_3.965604, whole genome shotgun sequence.
ACCESSION   DAOOJC010000278 DAOOJC010000000
VERSION     DAOOJC010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23081_length_3224_cov_3.965604"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..435)
                     /locus_tag="PLA01_07700"
     CDS             complement(79..435)
                     /locus_tag="PLA01_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HPD01214.1"
                     /translation="MEYFNGICFLKEGIAVQARIVDAYKMGVSVVAGKRYTVVYTDNN
                     GNEVRAEAILNTRDAKVGDVVTVRYLRDNPKKVYGDDSQGLGDVFFIGSIGLFFVGVL
                     IIYVMIKNRNKNQAIL"
     gene            complement(667..2889)
                     /locus_tag="PLA01_07705"
     CDS             complement(667..2889)
                     /locus_tag="PLA01_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPD01215.1"
                     /translation="MKRKRIKLHKILSMTVLVVILTTLLLQQFGLEPKVQAASSPRFG
                     DAYYNYGEALQKAILFYKANRLGDLPDDYILPYRADAAMTDGQDVGLDLTGGWADAGD
                     GIKFTHTISYAAAQLGWGVYEYREAFEKSGLLEDILDEVKWGTDFLIKANPEPNVLYY
                     MCGYGQSDHSVWVPHEFLDYVTDRKSFKVDPSAPGSDVAGMTAAALAIASLIFEETDP
                     EYAEKCLMHAERIFNFGYTYKGKNPLDILYPSGSYVDDLAWGAIWLYIKTGKQDYLDK
                     AISIMPVDTIGGYHTHCWDDVSYGAAIKIAQVTKDQKYASMVERNLDFFLPGGGITYT
                     PGGLAWLSQWGSLRYASTAAFLAFVWSDDETVGTLSKKDEYRKFAERQINYILGDNPR
                     GGSYEVGFGENPPVHPHHRTAHGSWVSMTDVPSFSRHILYGALVGGPNSSDYWEDNIE
                     DYVMNEVAIDYNAGFVGALAKMIDMYGGKKLENWPKPEDFRAPEDDLVEYFCRGWIMY
                     EGFGTLNLLLQVNNRSSWPPTVKDKMSVRYYMDLSEVFEAGGSLDDVEITLGQSDGAK
                     LEGLKHYADNIYYFTVDFSGTQIMPAEWNLCEKDANVSIRYKNGIGTHENDWSFQNLG
                     TSPDFDAISFAGLTPYIPVYDNGVLLWGEEPPYEGKTPNPTPNPTPTGPAFIYGDLNG
                     DEEVNSTDVTLLKRVILRKNPPGVNLSAADLNRDDSVDSTDLTILKRFILRKIESIPI
                     "
BASE COUNT          952 a          752 c          502 g         1018 t
ORIGIN      
        1 caattcatct cccacctaag aggttgggcg acttcttgca aagttcagtt aaataagtaa
       61 ggaagatact ttttattctc aaaggattgc ctgattttta ttgcggtttt taatcatcac
      121 ataaataata agaactccca caaagaaaag gcctatactg cctataaaga aaacatcccc
      181 caacccttgt gaatcatcac catatacttt tttcgggtta tcccttaaat atcttaccgt
      241 cactacatct ccgacttttg catccctggt attcagtatt gcttcagctc taacctcatt
      301 gccgttatta tcggtataaa ctacggtgta acgttttccg gctaccacac taacacccat
      361 tttataagca tcaacaatcc ttgcctgtac ggcaattccc tcttttaaaa aacaaatccc
      421 attgaaatat tccactcaaa aggcaaatca gtcccagtgc aaataaacta tcacactagt
      481 ccaattcatt ataatcttca cccccataac atcttttttc tgattatatc aactctctgc
      541 ctattctgcc actagttctt tgaaattaag agtaattttg tatatttcac ttcattatac
      601 ccgataaata taaaaaacct gcggatggtg aaatacttat ccgtaggttt ttcatactaa
      661 attacatcaa ataggtatcg actcaatttt ccttaaaata aaccttttca agattgttaa
      721 atctgttgaa tccactgaat cgtcacgatt caagtctgca gccgataagt taacgcccgg
      781 agggttttta cgaagtataa cccttttgag gagtgttaca tccgtcgaat ttacttcctc
      841 atcgccgttt aagtctccgt agataaatgc cggtccggtt ggcgtcgggt taggagtagg
      901 attaggtgtt ttaccttcat aaggcggttc ttctccccac aggagaactc cgttatcata
      961 tacaggtata tagggagtca atcctgcaaa agatattgca tcgaaatccg gagatgtgcc
     1021 caaattctgg aatgaccagt cgttttcatg tgtgcctatg ccatttttat atctaattga
     1081 tacattggca tctttttcac ataagttcca ttcggcaggc ataatctgag ttcccgaaaa
     1141 atcaacagta aaataataaa tattgtccgc ataatgcttc aatccttcaa gtttagcacc
     1201 atcactttga cctaaggtta tttccacatc atccaagctt cctccagcct caaatacctc
     1261 gcttaaatcc atgtaataac gaacagacat cttatctttc actgttggtg gccatgatga
     1321 cctattgtta acctgaagca ggagatttaa tgttccaaaa ccctcataca ttatccagcc
     1381 acggcagaaa tattccacca gatcatcctc aggtgcacgg aaatcttcag gcttcggcca
     1441 attttcaagt ttttttcctc catacatgtc aatcattttg gcaagagcac ctacaaaacc
     1501 tgcattgtag tcaattgcaa cttcattcat aacataatct tcaatattgt cttcccaata
     1561 atcactggaa ttaggaccac caaccaaagc accgtatagt atatgcctgc taaatgaagg
     1621 tacatcggtc atacttaccc atgaaccatg tgcagttctg tgatggggat gtacaggagg
     1681 attttctcca aaacctactt cataacttcc gccccgtgga ttgtcaccca atatataatt
     1741 tatttgtctt tctgcaaatt ttctgtattc atcttttttg gacaacgttc caacggtttc
     1801 atcatcggac catacaaaag ctaaaaatgc tgccgtactc gcataacgca aagatcccca
     1861 ttgggaaagc catgccaatc ctccgggggt gtaggttata cctccaccgg gcaagaaaaa
     1921 gtccagattt ctttccacca tagaagcata tttttggtct tttgtcacct gagctatttt
     1981 aattgccgca ccataactta catcgtccca gcaatgagta tgatatccgc ctattgtatc
     2041 gacaggcatt attgaaatag ccttatccaa gtaatcctgt tttcctgttt ttatgtaaag
     2101 ccaaatagct ccccacgcaa ggtcatccac atagcttccg gaagggtata aaatatccag
     2161 agggtttttc cctttgtatg tataaccgaa attaaaaatt ctctctgcat gcattaagca
     2221 tttttccgca tactctggat ctgtttcttc aaatattaga gaagctatag ccagtgccgc
     2281 tgcagtcata cctgcaacat cagaacccgg tgccgacgga tccaccttaa aggattttct
     2341 gtcggtcaca taatccaaaa actcatgagg aacccacacc gagtggtcag attgaccgta
     2401 tccgcacata tagtatagta cgttaggctc cggattggct ttaatcaaaa aatccgttcc
     2461 ccattttact tcatccaata tgtcctctaa caaccctgat ttttcgaagg cttccctgta
     2521 ttcatataca ccccatccca gttgtgctgc agcatatgaa atggtatggg taaacttaat
     2581 tccgtcgccg gcatcagccc aacctcccgt aagatcaagt cctacatcct gtccgtctgt
     2641 cattgctgca tctgccctat aaggcaaaat ataatcatct ggcaggtcac caagacggtt
     2701 ggctttgtag aaaagaattg ctttttgcaa ggcttctcca taattgtagt acgcatctcc
     2761 aaaacgtggt gaagaagcag cctgaacctt tggttcaaga ccaaactgct gtaacagcaa
     2821 agtagtcaaa attactacca gaacagtcat ggataaaatt ttatgcagtt taatcctttt
     2881 tctcttcatt ttgtttcctc cccttaaaaa cttttataat taattttaat attaaacagg
     2941 gcacaatatc tcgctttcga aatgtatgca aaggcataac aaagtattta gaagtgtttc
     3001 tttcatgtac gttattgctt ttggatttaa tttgctacat taattcgtca aaatattaaa
     3061 atttatagcc tctttatcta catctgcaat aaaattatat catatttttt tagtttctgc
     3121 ctctagtata tctactgctg ttgtcaattt ctatcttttt tattaaaacc caattaagac
     3181 acaaatcata gcctggagac aaaaataatt taactccata tccc
//
LOCUS       DAOOJC010000279         3219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_23134_length_3219_cov_3.742415, whole genome shotgun sequence.
ACCESSION   DAOOJC010000279 DAOOJC010000000
VERSION     DAOOJC010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23134_length_3219_cov_3.742415"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PLA01_07710"
     CDS             <1..464
                     /locus_tag="PLA01_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HPD01216.1"
                     /translation="GVTEAYKMQNIVYDALPNIENREDIFGQQIVMDEKDNITGYLKR
                     LLLTASSLSIDEAFMHVSSLGGVIIPAHVDRDSYSMISNLGIIPKTPQMKYLEVSKAC
                     DVEMFTKNNPYLKEFNIIKSSDAHTLGDIFERESSIELDELSIECLLNTLQ"
     gene            785..1279
                     /gene="nuoE"
                     /locus_tag="PLA01_07715"
     CDS             785..1279
                     /gene="nuoE"
                     /locus_tag="PLA01_07715"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPD01217.1"
                     /translation="MSTNKCCCCSGVDERDQKLQEIINKYKNTRGALIPVLHEAQDLY
                     GYLPLEVQKKIAQGLEVSLAEVYGVVTFYTQFSLNPKGKYKIQICMGTACYVKGAGAI
                     LDKLKEKLGLNVGECTEDGLFSLEACRCIGACGLAPVMMINDDVYGRLVPDDIEGILD
                     KYRE"
     gene            1294..1860
                     /locus_tag="PLA01_07720"
     CDS             1294..1860
                     /locus_tag="PLA01_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD01218.1"
                     /translation="MRDLSLHLLDIAQNSITAGASKIGVSICADKERDLLELEITDNG
                     IGMDECFLKEVVNPFVTTRDTRRVGLGIPLFEASARRASGELRVTSEKYVGTTVSADF
                     KISHIDRLPLGDIAQTLAALITARPEIEFKLILTNKKDSFDFNTSDVKIRLGEVPITE
                     PQVLTWICEYVNEGVKTIFGGVLDEILS"
     gene            1844..2215
                     /locus_tag="PLA01_07725"
     CDS             1844..2215
                     /locus_tag="PLA01_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPD01219.1"
                     /translation="MKSLAELEEIRKKTLEQISLRTDNHEMRVVVGMATCGIAAGART
                     VMNAFVEEFKKRNISDVPVTMTGCIGVCRLEPIVEIMDRDGSKVTYVKMTPEKVARVV
                     AEHIVNGRVCIDLTIGEADKK"
     gene            2247..>3219
                     /locus_tag="PLA01_07730"
     CDS             2247..>3219
                     /locus_tag="PLA01_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPD01220.1"
                     /translation="MQLYRAHVLVCGGTGCTSSNSEAIINELEAQINKNGLENEVKVV
                     KTGCFGLCAKGPIIVVYPEGSMYTMVKVEDVKEIVEEHLIKGRIVRRLLQSDKAEGVT
                     KSLEGVDFFKRQMRIALRNCGVINPENIEEYIAFDGYKALGKVLTEMTPQDVIDTLKK
                     SGLRGRGGGGFPTGLKWEFAAQQNSDQKYVCCNADEGDPGAFMDRSVLEGDPHSLIEA
                     MAIAGYAIGSNQGYVYIRAEYPIAVKRLKIAIEQAKKYGLLGNNIFGTDFSFDLDIRL
                     GAGAFVCGEETALMTSIEGHRGEPRPRPPFPAVKGLWQKPTILNNVET"
BASE COUNT         1005 a          487 c          796 g          931 t
ORIGIN      
        1 ccggtgtgac tgaggcatat aagatgcaaa acattgttta tgatgccctc cctaacattg
       61 aaaaccggga ggatatattc ggacagcaga ttgtgatgga tgagaaggat aacattacag
      121 gatatcttaa aagacttctt ctgactgctt caagtcttag tattgatgag gcttttatgc
      181 atgtttcttc actgggggga gttataattc ctgcccatgt agacagggat tcgtacagta
      241 tgatttcaaa tctgggaatt attccgaaaa caccgcagat gaaatatctt gaggtttcaa
      301 aagcatgtga cgtggaaatg ttcacaaaaa acaatccgta tcttaaagaa ttcaatatta
      361 taaagtcgtc ggatgcccat acgctggggg atatatttga aagagaaagc tctatcgagc
      421 ttgacgaatt aagcattgag tgccttttga acactcttca gtaaatattg aagcttagtt
      481 ggtacaattt acaccgcctt tagtctggta tcaatagttt gcgaaataat cattcaatca
      541 gataatatta tgcaatcagt taatattgaa tattgtatac aatttcgaca tgcaattttt
      601 atttttcaaa cttgcataaa tccttgtgaa ttaaagcttt gagtatgttt ctaaatctat
      661 aaaatattat caaaatctat tgaatgttat attaatatct gttataattg taacgaaatt
      721 tgttaaattg ttaacataca cttttttgat aaaataatta cctggttagg gggttgttgg
      781 taaaatgagc acaaataaat gttgctgctg cagcggagtt gacgaaaggg atcaaaagct
      841 gcaagaaata atcaataagt acaaaaacac aaggggagcc ttgattcctg tattgcatga
      901 agcacaggat ctgtacggtt atcttcccct ggaggttcag aagaagattg ctcaagggct
      961 tgaggtatct ttggcagagg tttacggtgt agttactttc tatacccagt tttctctgaa
     1021 tccgaaagga aagtacaaga tacagatttg tatgggtaca gcatgctatg ttaagggagc
     1081 tggggctatt ttagacaagc ttaaagaaaa gcttggcctc aatgtcggag aatgcaccga
     1141 agatgggttg ttttcgttgg aagcatgcag atgtattggt gcctgcggct tggcgccagt
     1201 aatgatgatt aatgatgatg tctacggccg tcttgtacct gacgatattg aaggaatact
     1261 tgataagtat agggaataaa gagtttaaga ataatgaggg atttgtcatt acatttgctg
     1321 gatattgcac aaaactctat tactgccgga gcaagcaaga ttggtgtttc aatttgtgca
     1381 gataaagagc gggatttgtt ggaactggaa ataacggata atggtatagg catggatgaa
     1441 tgctttctga aggaggttgt caaccccttt gtaaccacaa gggataccag acgggttggc
     1501 ttgggtattc cgcttttcga agcttctgca agacgggctt cgggtgaact cagggttact
     1561 tccgagaagt atgttgggac aacggttagt gcagatttta agatatcgca cattgacagg
     1621 cttcctttag gcgatatagc acagacattg gcagctctta ttactgcaag acccgagatt
     1681 gaatttaaac tgattcttac taataagaag gacagttttg actttaatac atcggatgtg
     1741 aaaataagac tgggagaggt tccgatcacc gagcctcaag tgttaacgtg gatttgtgag
     1801 tatgttaatg agggtgtaaa aactatattt ggaggggtgc tagatgaaat ccttagctga
     1861 attagaagaa attaggaaaa agactctgga acagataagt ttaaggactg ataatcatga
     1921 aatgagagtc gttgtgggta tggctacctg cggaattgca gctggagcaa gaacagtaat
     1981 gaatgccttt gttgaggagt ttaagaaaag aaacatcagt gatgtgcctg ttactatgac
     2041 gggttgcatt ggagtttgca ggcttgaacc tattgttgaa ataatggata gggacggaag
     2101 taaagtaact tatgtcaaga tgactcctga aaaggttgca cgtgttgttg cagaacatat
     2161 tgtcaacgga agggtatgta ttgatttaac tattggagaa gcagacaaaa agtaactgtt
     2221 tttatttttg ttaaggaggg ttataaatgc aattatatag agcacatgtt ctggtttgcg
     2281 gtggtactgg ctgtacttcg tcaaactctg aagcaataat aaatgaattg gaagcacaga
     2341 tcaataaaaa tgggctcgaa aacgaagtta aagttgtaaa gaccgggtgt tttgggcttt
     2401 gtgccaaggg acctatcatt gttgtatacc ctgaagggtc aatgtacaca atggtcaagg
     2461 ttgaggatgt aaaggaaatt gtggaagagc accttataaa gggtagaata gtaagaagac
     2521 tattacagag tgacaaggcc gaaggagtaa ctaaatccct tgaaggtgtg gatttcttca
     2581 aaagacagat gagaattgca ttgagaaact gtggtgtgat aaatcctgaa aacattgagg
     2641 aatatattgc ttttgacggt tataaggcat tgggcaaagt tttgacagag atgactccac
     2701 aagatgttat tgacactctc aaaaagtccg gcttaagagg aagagggggc ggaggtttcc
     2761 caacagggct taaatgggag tttgctgcac agcaaaactc agatcagaaa tatgtatgct
     2821 gtaatgctga cgaaggggac cccggtgcat tcatggacag gagtgtgttg gaaggagacc
     2881 cccactcttt aattgaagct atggcgattg ccggatatgc tatcggttca aatcagggtt
     2941 atgtttatat cagggcggaa tatcctatag cggttaagag gcttaaaatt gctattgaac
     3001 aggcaaagaa atatggactt ttgggcaata atatatttgg aaccgacttt agctttgatc
     3061 ttgatatcag gcttggtgcc ggggcttttg tatgcggtga ggaaaccgct cttatgacat
     3121 ctattgaggg acatagaggt gagccgagac caaggcctcc gttccctgcg gtaaaaggtt
     3181 tgtggcaaaa gcctacaata ctgaataacg tagagaccc
//
LOCUS       DAOOJC010000280         3219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_23136_length_3219_cov_3.219975, whole genome shotgun sequence.
ACCESSION   DAOOJC010000280 DAOOJC010000000
VERSION     DAOOJC010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23136_length_3219_cov_3.219975"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PLA01_07735"
     CDS             complement(<1..676)
                     /locus_tag="PLA01_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPD01221.1"
                     /translation="MKQKKASSDNRKKKTGKKMSFKKSLFIWIGSFVVLGVLIFVFGK
                     FVLGSSNGGSDLDLEITYPPIKTISPTPTRLSSITPSATPEEIDYDSIPGLTNGMKYP
                     EKLVDENSRNIMFIGQDRVSGLYDTIGILSIDKKNEKLKIIMIPRDLYVDYNAKVRHY
                     MELNDRVNDPDFYKINSAYLIGPFMKQEGKFGAYSMNFLAALMKEIFDIEVHDYVRVN
                     TEGFVNI"
     gene            complement(916..1545)
                     /locus_tag="PLA01_07740"
     CDS             complement(916..1545)
                     /locus_tag="PLA01_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01222.1"
                     /translation="MSDFLKDILEAINEISSEVNQSKNMQGNNMDRNPYPRNLSSQPK
                     PPGPSYYNRPLRSEPKPGYGTTPRPQVNYGVGRTNIPNPSFEGVPGHEGNFGFEGSSG
                     FEGTPGYEGNSLPQTYSDVVLEDSPGVEGSPGTEGSSGFEGSPGSEGREAVNKPVSAK
                     KGKVTARLNENSIFETALDFSKLTEGDVIRGIVFSEVLGKPKALRRGRW"
     gene            complement(1569..2564)
                     /gene="floA"
                     /locus_tag="PLA01_07745"
     CDS             complement(1569..2564)
                     /gene="floA"
                     /locus_tag="PLA01_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837919.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HPD01223.1"
                     /translation="MEYGFLGFILIFGAAIVLLVFFFTFIPVGLWISAIAANVRVGIF
                     TLVGMRFRRVIPARVVNPLIKATKAGLTVSINKLEAHYLAGGNVDRVINALIAAQRAS
                     IPLEFERAAAIDLAGRNVLEAVQMSVNPRVIETPIVAAIAKDGIELRTKARVTVRANI
                     DRLVGGAGEQTIIARVGEGVVTTVGSAMNHKEVLENPDAISRTVLSKGLDAGTAFEIL
                     SIDIADIDVGRNVGAQLQTDQAEADKRIAQAKAEERRAMAVAREQEMKAMVQEMRAKV
                     VEAEAEVPKAMATALREGKLGVMDYYNMQNILADTQMRNSISKAGKQDEPTIEKK"
     gene            complement(2642..3136)
                     /locus_tag="PLA01_07750"
     CDS             complement(2642..3136)
                     /locus_tag="PLA01_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPD01224.1"
                     /translation="MFDFIYNIDIWIALFFIIGFLLVIIEIFNPGFGIPGALGAIFLV
                     IGVVLTMDTILDAIIMIVAILIVLAVAFFLAIYSVKKGLLSRTIVLTDSLDKESGFQG
                     TEDLKAFLGKEGIALTALRPAGTAEFNGTRVDVVSEFDYIEKDSKVKVIDVEGRRVVV
                     RKIK"
BASE COUNT          911 a          714 c          541 g         1053 t
ORIGIN      
        1 ctatattaac aaagccttcg gtattaactc tcacatagtc atgaacctca atatcaaaaa
       61 tttctttcat tagagccgcc aagaagttca ttgagtaagc accgaatttg ccctcctgct
      121 tcataaaagg tcctattaag taggcactgt taattttgta gaaatcaggg tcatttaccc
      181 tgtcgttcag ttccatgtaa tgtctgactt ttgcgttgta gtcgacatac aaatctcttg
      241 gaatcattat tattttcagc ttttcatttt ttttgtcaat gctcagtatt ccgatagtgt
      301 catataatcc cgatactctg tcctgaccga taaacataat atttcggctg ttttcatcta
      361 caagcttttc aggatatttc attccgtttg tcaacccggg aatcgagtca taatcgattt
      421 cttccggtgt tgcacttgga gtaatactgc ttaacctggt gggggtaggt gaaatagttt
      481 ttataggagg atatgtaatt tccaaatcta agtcggaacc gccgttactg cttccaagaa
      541 caaacttgcc aaaaacaaaa attaatactc ctaaaactac aaaactaccg atccatataa
      601 ataaagattt cttaaaactc atttttttgc ctgttttttt ctttctatta tcagatgatg
      661 cttttttttg tttcattata aaagacccct caataattaa taaaatttaa catttgcata
      721 ataaatctat atttcaaaca ttaatatttt aacacatatt aatattaaaa gggatactaa
      781 aatcgtattt aaaccaaatt taaaattttt gtaataattt gctcaggtgt gtcgtaaaca
      841 ggtgaaaatc aaaagacgaa acaaaagcga tgggagcatc gaaccccatc gctttttggc
      901 catttcactt ataatttacc atctgcctct tctcaatgct ttaggcttac ctaatacctc
      961 ggaaaaaact attccgcgaa tgacatctcc ttcggtaagc tttgaaaaat ccaaagcagt
     1021 ttcaaagatt gagttctcat taagcctggc tgtgactttg ccttttttag ctgaaacagg
     1081 tttattcaca gcctcacggc cttcgctgcc ggggcttcct tcgaatccgg aactgccttc
     1141 agtaccggga ctgccttcaa caccgggact gtcttcaaga acaacgtctg aatatgtttg
     1201 cggcagagaa tttccttcat atccgggtgt gccctcaaag cctgaactgc cctcaaagcc
     1261 gaagtttcct tcatgaccgg ggactccttc aaagctcgga ttcgggatat tagtccttcc
     1321 aaccccgtaa ttgacctgag gacgtggtgt tgttccatat ccgggtttgg gctcacttct
     1381 taaaggccta ttataatagc tcggcccagg tggttttggt tggctggaaa gattccgtgg
     1441 gtatggattt ctatccatgt tattgccttg catatttttg ctttgattca cttcggagga
     1501 aatttcatta atagcctcca atatatcctt taaaaaatca ctcattaata aaacagctcc
     1561 ttaagctttt atttcttttc gattgtcggt tcatcctgtt tgcctgcttt tgaaatgctg
     1621 tttctcattt gggtgtcagc caatatgttc tgcatattgt agtaatccat gacaccgagt
     1681 ttaccttctc taagggctgt tgccatagct ttaggtactt ctgcctctgc ctctacaacc
     1741 ttggccctca tttcctgaac catggctttc atttcttgtt ctcttgcaac tgccatagct
     1801 cttctttctt cagcttttgc ttgggcgata cgcttatcag cctccgcctg gtctgtctga
     1861 agctgggcac ctacatttct tcctacatca atgtcagcta tatcaattga gagtatttca
     1921 aatgctgttc ctgcatcaag gcctttgctt aatactgttc tggaaattgc atctgggttt
     1981 tcaagaactt ctttatggtt cattgccgaa ccgacggtgg taacgacacc ctcgccgaca
     2041 cgggcaatta tggtttgttc accggcacca cccaccaagc ggtctatgtt tgctctaaca
     2101 gtaactctgg cttttgtacg aagctcaata ccgtctttgg cgatagcagc aacaatcggt
     2161 gtttcaatta ctctgggatt tacgctcatt tgaactgctt caagaacatt tcttcctgca
     2221 agatcaatgg ctgctgccct ttcgaattca aggggaatac tggctctctg tgcagctata
     2281 agtgcgttaa taaccctgtc aacattacct cctgctagat agtgtgcctc aagtttgttt
     2341 atcgacacag taagacctgc ttttgttgcc ttaataagag gatttaccac tctggcaggt
     2401 ataactcttc tgaaacgcat tccgacaaga gtaaatatac cgaccctaac atttgctgca
     2461 atggcggaaa tccaaagacc tacagggata aatgtaaaaa agaaaaccag taacacgatt
     2521 gccgccccaa aaatgaggat aaaacctaaa aaaccatatt ccatactaaa actctccctt
     2581 cgtgtttatt aaaatttttg ttatattata ttttagaaat aatacaatat ccgtaaaaca
     2641 attattttat ttttcttaca acaacacgcc gtccttcaac atcaataact tttacttttg
     2701 aatccttttc aatgtaatca aactcagata caacatccac tcttgttccg ttaaattcag
     2761 cggtacctgc aggacgcaat gctgtcaagg ctattccttc ttttccaaga aaagctttca
     2821 gatcctctgt tccctgaaaa ccgctttctt tgtccaatga atcagtcaat acaatggttc
     2881 tggatagtaa gccctttttt actgagtata ttgccaggaa aaaagcaacg gccaatacta
     2941 taagtatagc tacaatcatt attatggcat ccaaaatagt atccatggtt aataccactc
     3001 ctattacaag aaagattgca ccgagtgcac ccggtattcc gaaccccgga ttaaatattt
     3061 ctattatgac cagcaagaat cctattatga agaataatgc aatccagatg tcaatattat
     3121 aaataaagtc aaacatgtga acaaccccct ttttaagaaa ttttaattag ttgtttgtaa
     3181 aatgctgtaa cccttgaaac caaagggttt tagcagtat
//
LOCUS       DAOOJC010000281         3214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_23167_length_3214_cov_4.017411, whole genome shotgun sequence.
ACCESSION   DAOOJC010000281 DAOOJC010000000
VERSION     DAOOJC010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23167_length_3214_cov_4.017411"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2583)
                     /gene="smc"
                     /locus_tag="PLA01_07755"
     CDS             complement(<1..2583)
                     /gene="smc"
                     /locus_tag="PLA01_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPD01225.1"
                     /translation="MHLKRLEIQGFKSFADKIALDFNSGITAVVGPNGSGKSNISDAI
                     RWVLGEQSAKTLRGGKMEDVIFAGTEHRKPLGFAEVTLTFDNSDSMLPVEFSEVTVTR
                     RVYRSGESEYMINKTQCRLKDIYEMFLDTGVGKDGYSIIGQGRIDEILSSKSDERRHI
                     FEEASGIMKYKVRKQEAEKKLEMTRQNLLRINDIIGELEIQLEPLREQAEVAKKFLSL
                     RESLKELEVNVYIENITRFKEKLEEFEKSYSTINQNIESENNKLEEITNLNQKNISIL
                     KDMDSRLEQAKQEFYAIDGNLEKCGSEIRLNEEKKSNLISNISRLDGEIAEIEEKIKG
                     LSGEEEQKNNKIKYLNERYGEYSLKLEESEKQLKAILDTLDENERYIENLKSSIMEKL
                     DIQSDKRTQINNVKNHIEIIRKRQASIDEEQNKLILEKDREGMKKEDLSEGISKVNEL
                     IKNIKEQLDALYEKRKDLGLKLEEEKKSQNNVRSEIQIKTSRLRMLKEMEKNLEGYNR
                     SVKVVLQACQQSPDFGRGIHGALAQLITVDKRYETAIEMTLGGALQNIVTTSEEDAKR
                     AIEFLKKNRLGRATFLPISAVKGRYIEDSIVNQLKGQEGFIGVASDLISCSPEYRGII
                     LSFLGKVVVVENLDTAIKMARRYGYGFRIVTLEGDILSTTGSMSGGSNTQKESGILGR
                     NREVSELSEGIEKLKSDEEVLEKNVSEISFMLSDIVDQISDNESSLKNNELIKIRDES
                     HLAQVDENIKRILARVDMLKQEKEQLARQEKEIGEELKKYEEELTLIEKDIEETRNKI
                     ALNQEKSKEQQSIRDKLHADITDYKISVNSILESTEAVKESLERIQNERNSHTKGLER
                     RIAEK"
     gene            complement(2891..>3214)
                     /locus_tag="PLA01_07760"
     CDS             complement(2891..>3214)
                     /locus_tag="PLA01_07760"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPD01226.1"
                     /translation="PAVREAVAMGYRVEGPIGADSVFHLALMGRYNSVLSLYHDQGHI
                     ATKTLDFERTIAITVGLPFLRTSVDHGTAFDISGSGKASAVSMVEALLKAGKYVRSYS
                     KNISE"
BASE COUNT          771 a          815 c          476 g         1152 t
ORIGIN      
        1 tttctccgca atccttctct ctaacccttt tgtatggctg tttctctcat tttgaatcct
       61 ttcaagagat tctttcactg cttccgtact ctccaatatt gagttaacgg aaatcttgta
      121 atcggttata tcggcatgga gcttgtccct tatgctctgc tgctccttgc tcttctcctg
      181 attaagggct attttgtttc tggtctcttc aatatctttt tctataaggg tcaattcttc
      241 ctcgtacttt ttgagttctt ctcctatttc cttctcctgc cttgcaagct gttccttctc
      301 ctgcttgagc atatctaccc ttgccaatat cctctttata ttctcatcca cctgtgccaa
      361 atggctttca tccctaattt ttatcaattc attgttttta aggctgcttt cgttatcgct
      421 tatttggtct acgatatcac tgagcatgaa acttatttcg cttacattct tttccaacac
      481 ctcttcatca gacttgagct tttcaatacc ctccgaaagc tccgaaacct ctctgtttct
      541 ccccaaaatt cccgattctt tctgggtatt gctacctccc gacatggaac cggtagtact
      601 taaaatatct ccctcaaggg taacaattcg aaaaccgtat ccgtatcttc ttgccatctt
      661 tattgccgta tcaaggtttt ctaccactac cacctttccc aaaaagctca gtataatacc
      721 cctgtattcc gggctgcagc ttataagatc cgaggcgaca cctataaaac cttcctggcc
      781 tttgagctgg tttacaatac tgtcttcaat atacctgccc tttactgcgg atataggcag
      841 aaaggttgcc cttcccagcc ggtttttctt taagaactca atggctcttt tggcatcctc
      901 ctcactggta gttacaatgt tttgaagtgc tcctcccaaa gtcatctcaa tagcagtctc
      961 atacctttta tcaacggtta tcagctgtgc caatgcacca tgtatacctc gtccaaaatc
     1021 cggggattgc tggcatgcct gtaaaacaac cttgacactc ctgttatagc cctcaaggtt
     1081 tttctccatt tctttgagca ttctaagtct tgaggtcttt atctgtattt cagacctcac
     1141 gttattctga cttttcttct cttcctccag ctttaaaccc aaatccttcc ttttttcata
     1201 caaagcatcg agctgctctt ttatgttttt aatcaattca ttgactttag aaataccctc
     1261 agacaggtct tccttcttca tgccctccct gtccttctct aaaataagct tattttgctc
     1321 ttcgtcaatg cttgcctgcc ttttccggat aatttcaatg tgatttttca cattatttat
     1381 ctgagtcctt ttatcggact gaatatcaag cttttccatt atgcttgatt ttaagttttc
     1441 aatatacctc tcattctcgt ccaatgtatc caatatcgct ttaagctgct tttcactttc
     1501 ctcaagcttt aatgaatatt caccatacct ctcgtttaaa tacttgattt tgttattctt
     1561 ttgctcctct tccccggaca atcccttgat tttttcctca atctcagcta tttctccatc
     1621 gagtcgtgaa atgtttgaga tgagattgct ctttttctcc tcattcagcc ttatttccga
     1681 tccgcacttt tccagattgc cgtcaatggc gtaaaactcc tgctttgcct gttcaagcct
     1741 gctgtccata tcctttagaa tacttatatt tttctgattg agatttgtaa tttcctcaag
     1801 tttattgttt tcactttcga tgttttgatt tattgtagaa taactttttt caaactcctc
     1861 caacttctct ttaaaccttg taatgttttc aatataaaca ttaacctcca gttccttcag
     1921 gctttctctg aggcttaaaa acttttttgc aacctctgcc tgctccctta agggttcaag
     1981 ctgaatctca agttctccaa tgatgtcatt tatccttaaa agattctgtc ttgtcatttc
     2041 cagctttttt tccgcttcct gcttcctgac cttatacttc attataccgg aagcctcttc
     2101 aaatatatgt cttctctcat cggatttgga acttaggatt tcatcaatcc ttccctgccc
     2161 gatgatggag taaccgtcct tgcccacacc ggtatccaga aacatctcgt aaatgtcttt
     2221 aagccggcat tgtgtcttgt ttatcatata ctcactttca ccggagcggt acactcgcct
     2281 ggttacggta acttcactga attcaacggg aagcatggaa tcagagttgt caaaagtgag
     2341 ggtaacctcc gcaaagccca gaggttttct gtgttctgtc ccggcaaata tgacatcctc
     2401 catttttccc cctcttaggg tttttgcact ctgctctccc agaacccatc tgattgcatc
     2461 ggaaatattg ctttttccgc ttccgttagg ccccacgaca gcagtaatac cggagttaaa
     2521 atcaagtgca atcttgtctg caaaggattt aaagccttga atttcaagtc tttttagatg
     2581 caaataaaac acctcgtatt atagaataaa gtagcttcaa tcatataaaa aaccacgcaa
     2641 aatcaaaatt gcatgggtaa tttaatacat aataattcta acataatttt atattttatc
     2701 aatacacaca atttcactac taaaaaacaa ctcagtttca aaattttcag acatattatt
     2761 atacgtctta aagctctacc gtattttata ccaaacactc atatcccttt actctgatat
     2821 gttttttgaa taattgtgca catattccct tactccgata tgctttttga ataaccgtgc
     2881 acttataccc ttactccgat atgttttttg aataactgcg aacatatttc ccggctttga
     2941 gaagagcttc aaccatacta accgcacttg cctttcctga acctgaaata tcaaaagcag
     3001 tgccgtggtc aacggatgtg cgtaagaacg gcaatcctac ggtaattgca attgttcgtt
     3061 cgaagtccag ggtttttgtg gcaatgtgtc cctggtcatg atacagggac agaaccgagt
     3121 tgtaccttcc cattaatgca aggtgaaata ccgaatccgc cccaataggt ccctcaaccc
     3181 tgtagcccat ggcaactgcc tccctgactg ctgg
//
LOCUS       DAOOJC010000282         3213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_23179_length_3213_cov_3.537682, whole genome shotgun sequence.
ACCESSION   DAOOJC010000282 DAOOJC010000000
VERSION     DAOOJC010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23179_length_3213_cov_3.537682"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..739
                     /locus_tag="PLA01_07765"
     CDS             161..739
                     /locus_tag="PLA01_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01227.1"
                     /translation="MDYYFCPFVSEPYAAYTPYVWQSQNIDESYLNSVPATERQPQPV
                     LSDRPAQISVVLFKELTGYPNYGNPSGNADILYTGNRGTWTFNLQPFLLLQSNLSAQL
                     VIQGVLDDHTNVPVANYSARITINGTVVHTGRLPFVHGSPTGQRFNNWRSLTFNVPNL
                     RSNNRVVIENTSNTGPNDWIAFDWMELRLQPR"
     gene            complement(854..2217)
                     /locus_tag="PLA01_07770"
                     /pseudo
     CDS             complement(854..2217)
                     /locus_tag="PLA01_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518875.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
     gene            complement(2591..3031)
                     /locus_tag="PLA01_07775"
     CDS             complement(2591..3031)
                     /locus_tag="PLA01_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HPD01228.1"
                     /translation="MINKTGQTAKNIISEHSNLVTIIMGILVSYTITLPIAAVFAYIL
                     MFTDFPQKYLSTAVIITTLLSIVIAGWVSTKNVKSKGWLNGGIVGIVYVLILYLVSSV
                     AYRDFSVNMHVVTLFFIGILTGSIGGILGINLRREPRAVTRSRK"
BASE COUNT          950 a          753 c          540 g          970 t
ORIGIN      
        1 ataattcata gaaaacagat tctgcatccc tgtttacggg ataccctgac catcctttgg
       61 aatcaaatta gtgtaatcct ttgcataatg gtaacataac tctctatttc tcatattatg
      121 tatagtagaa agctatttca atatgaaagg agaaatcatt atggattact acttctgccc
      181 ttttgtatct gaaccatacg ccgcatatac accatatgta tggcagtctc agaacatcga
      241 tgaatcgtac ttgaactccg ttcctgccac agaaaggcaa cctcaaccgg tactgagtga
      301 cagaccggca caaatttccg tcgtgctttt taaggaactc accggttacc caaactacgg
      361 aaaccccagc ggtaatgcag acattttata taccggaaac cgtggtacat ggacctttaa
      421 tttacagccc ttcttactct tacagagcaa cttgagtgca caactggtaa tacaaggtgt
      481 ccttgacgac cacacaaatg ttcctgtagc caattactcg gccagaataa caataaacgg
      541 tactgttgtc catacgggca gacttccttt tgttcacggt tcacctaccg gacagcgttt
      601 caacaactgg aggtctctta cctttaatgt accgaatctg agaagcaata acagagtggt
      661 aattgaaaat acctcaaata caggtccaaa tgactggatt gcttttgact ggatggaact
      721 aaggcttcag cctaggtaaa acaatccttt cactacaaaa ggccaaatat aaacaacccc
      781 ttttttcttt cgaaagcagg ggttattttc caattttact taaattttat taactcttta
      841 attaattaac attttactca accttcggca ggctgttcat actcttcgaa attgcttcct
      901 cagaataatc tatatcgcta agctttccgg aaaagtagtt gtcataggca accatgtcaa
      961 aatacccgtg tccgctcaga ttaaacagaa taacttttga ctctcccgac tccttgcata
     1021 ataaggcttc gtcaacagca gcctttattg catgggaaga ttccggagcg ggaataattc
     1081 cctcagttct tgcaaaagca acagcagcct caaaaactga actctggcca taagctttga
     1141 cttcaatcag cttgtcacta taaagctgac tgattatcgg cgaaactccg tgatatctaa
     1201 gtccgcctgc atgtataccg gcaggtacaa agtcatgacc cagtgtgtac atttttgcca
     1261 acggtccaac ctttcctgta tcggaataat cataagcata cacacctttt gtaagggtcg
     1321 gacaggcagt tggctcaaca gccacagctc tcacctttgt tcctttaaac ttgtccatca
     1381 ggaatggaaa agctattccg gcaaagttcg atcctcctcc gcaacaagca aataccacat
     1441 ccggatattc atccagatac tccagctgtt tcttcgcctc gagtcctata atggactggt
     1501 gcaggcaaac atgatttaaa acgcttccca atgcataatt ggtatcattg tgagtagcag
     1561 catcttcaac agcttcactt atcgcaatac caagacttcc ggtacagtca gggtcctttt
     1621 ccaaaatcgc cctgccgctg ttggtaagat tagtaggact tgcatacact tccgccccaa
     1681 aagttttcat aaaggaacgt ctgtaaggct tttgctcata acttacctta accatataga
     1741 ccgtacactc aagcccgaaa tgactgcatg caagactaag tgcacttccc cactgtccgg
     1801 caccggtctc cgtggaaagt ctttttattc cggcaatctt gttgtagtaa gcctgaggca
     1861 gtgatgtatt aagcttgtgg cttccggtag catttgtccc ttcatatttg taataattct
     1921 cgcaggagtc cccaaatatt tttcaaggcc ttttgccctg tataatggac ttggtctcca
     1981 ttgacggtac atttccctta cttcctcagg aatgtcaatc catctctcct gggaattttc
     2041 ctgttgaatc agttctagtg ggaagattgc ctgaagttca tcggcagaag ctattttacc
     2101 tgtttgggaa cttatgtaag gtgcgggttt gttgggcatg tctgccaaaa tattgtacca
     2161 ttgtttcgga atatccgact cgtctagaac tactttgaaa acatcctttt tgctcatctt
     2221 tctcaactct cctcatcaaa ttaataacac taattatatt gtacgaaaaa aagccgcaac
     2281 aatcaacaaa aaagataaaa tttcaactta aaaatccaag atttataaat atactattac
     2341 acagcaaaat aattgatttg cagaattttc tgaattagcc ccctataaaa caaaagctcc
     2401 gggagcatcg agcccccatc gctttttggt catttcactt cgtgaaatcc cactaagaaa
     2461 gtgctaaact acatactata agtttagcac atttttttat ttttaatccc aaagtggagc
     2521 aatattaaca taatgtaagt ttttgccctt actttcttta ttatttttta tgtatttatg
     2581 atccaacaat ttacttcctg ctcctggtaa ctgcacgggg ttcccttctt aaatttatgc
     2641 ccagtattcc gccgatgctt cctgtcaaaa tccctatgaa aaacagggta acaacatgca
     2701 tgttaacgga aaagtctctg tatgccacac tgctcaccaa atagagaatc agcacgtaca
     2761 caatacctac aattccgccg tttaaccatc ccttgctttt tacatttttt gtagacaccc
     2821 atcctgctat cacaatactt aaaagtgtcg tgattataac agccgtagac agatacttct
     2881 gcggaaaatc cgtaaacatc aaaatatacg caaaaaccgc agcaattggc aatgtaatgg
     2941 tataggacac aagtatcccc attattattg tcaccaggtt ggaatgctca cttataatgt
     3001 tcttcgccgt ctgtccggtc ttattaatca taacaacccc ccaatagatt taaagtttca
     3061 tactaataat ctattttatt ttatttaaag atagaaccga taaaaccacc ggtaaaaata
     3121 tctatgcgat ttatttactt ggcatcccat tccaaaagtt gcttatacct ttgcttaatg
     3181 cttagtccgc agagtttttg actttattga acg
//
LOCUS       DAOOJC010000283         3208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_23230_length_3208_cov_2.584840, whole genome shotgun sequence.
ACCESSION   DAOOJC010000283 DAOOJC010000000
VERSION     DAOOJC010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23230_length_3208_cov_2.584840"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLA01_07780"
     CDS             <1..483
                     /locus_tag="PLA01_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HPD01229.1"
                     /translation="LGEALYVDNTLMFEDDEEKDKDNKEDKKEDKKGENKALQASKEA
                     NDFFAQAKMDKDITRAKDREVLEEIANDENATEEQRAEAYEKMLSLIDNSDKEMRIET
                     LIKNKGFNDVVVLFGDNGSIDIVVKAPSISSVEVAQISEIASRQTGVDISNIYVSNKF
                     "
     gene            640..1044
                     /locus_tag="PLA01_07785"
     CDS             640..1044
                     /locus_tag="PLA01_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HPD01230.1"
                     /translation="MDEKNRDSLNEMGTVKISEEVVAIIAGIAAMDVPGVAGMSGGIA
                     GGIAEMLGRKNLSKGVKVEVGDKEAAIDLYIIVEYGCRIPDVSWDIQERVKKAVETMT
                     GLNAIEVNIHIQGVNIDKDSRKESGDDTLRLR"
     gene            1181..1768
                     /gene="amaP"
                     /locus_tag="PLA01_07790"
     CDS             1181..1768
                     /gene="amaP"
                     /locus_tag="PLA01_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HPD01231.1"
                     /translation="MGKLKIKKGGGCRINIIFRILLAVYAFCLTVISLITLVLTLRPE
                     MIERASKLLVNDVLPNRGLTLLMLFIEVAFLGLSLMFLLSGVRSEKDKKSVSKYNNVG
                     EIRISLNAIESIALNASKKVSGIRENKAYVTKQGDHVSVFIKTIVMPDVNIPALLEDI
                     QVKVKKSVEESTGIAVNEVRASVENIYTGYKSRVE"
     gene            1783..2025
                     /locus_tag="PLA01_07795"
     CDS             1783..2025
                     /locus_tag="PLA01_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HPD01232.1"
                     /translation="MDKQEIMKKIIGFYRLHYGGINGAFIGLVVAICVLVIGFFQTLF
                     IAICVLVGYYIGKKISKDKNYLRNLLDRFLPPGTYR"
     gene            2066..2491
                     /gene="nusB"
                     /locus_tag="PLA01_07800"
     CDS             2066..2491
                     /gene="nusB"
                     /locus_tag="PLA01_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPD01233.1"
                     /translation="MGRKASREIAMKLLYQLEFQKDSREEQIEVALEQYNLNSKDREY
                     IIDIINGVYNNKESIDSTIEKHSRGWKLSRISKVDLSILRLSIYEMCYRKDIPLNVSI
                     NEAVELAKNYSGGESGSFVNGILGNVAKIRLLLVDGNDE"
     gene            2493..>3208
                     /gene="xseA"
                     /locus_tag="PLA01_07805"
     CDS             2493..>3208
                     /gene="xseA"
                     /locus_tag="PLA01_07805"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPD01234.1"
                     /translation="MNNILTVSQVNRYIKENMSRDLILSNLWIRGEISNYKNHSSGHM
                     YFTLKDENSLIRCVMFRTYNSYLKFKPENGMKVIIRGYISVFERDGQYQLYAEEMQSD
                     GVGNLHISFEQLKKRLSDEGLFDAAYKKKIPYLPRSIGVVTSSTGSVIRDIMNVLDRR
                     FYNSVIKIFPVKVQGDSACGEICHAIEKLNESGCVDVIILARGGGSLEELWPFNEESV
                     ARSIFKSKIPIISAVGHETD"
BASE COUNT         1072 a          440 c          771 g          925 t
ORIGIN      
        1 ttaggggaag ctttgtatgt ggataacaca ttgatgttcg aagacgacga agagaaggat
       61 aaggataata aagaggataa aaaagaggac aaaaaaggtg aaaacaaagc tttgcaagca
      121 tcaaaagagg caaatgattt ctttgcccag gcaaaaatgg ataaggatat aacgagagcg
      181 aaggacagag aggtattaga agaaattgcc aacgatgaaa atgcaaccga agagcagaga
      241 gccgaggcat atgaaaaaat gttgagcctg atagataatt ccgataaaga aatgagaatt
      301 gagacgttaa ttaaaaacaa gggtttcaat gatgtagtag tgctttttgg ggataacgga
      361 agtattgata ttgtagtgaa ggctccgagc atatcatcgg tagaagtagc tcaaatatcc
      421 gaaatagcat ccagacagac tggggtagat atatcaaata tatatgtatc caataaattt
      481 taacaaattt taagcttttt aattggttag gaaccggttt ctgtcagcgt tttttatgtg
      541 catttatttc ccggtgttga taataaaaaa acatagtgct ataataaatt gtattggcaa
      601 atcttaacgg ctgtattcca gcaaaggagg tatcataaag tggacgagaa aaatcgggac
      661 agcttgaatg aaatgggaac tgtgaaaata tcggaagagg tagtcgctat tattgcgggc
      721 attgctgcaa tggatgttcc gggagtggct gggatgagtg gtgggattgc cggtggaatt
      781 gccgagatgc ttggaaggaa gaatttgtcc aaaggagtaa aagttgaagt cggcgacaaa
      841 gaagccgcaa tcgatttgta tataattgtt gaatatggct gcagaattcc cgatgtatcg
      901 tgggatatcc aggagagggt taaaaaagct gttgaaacca tgacgggtct taatgcaata
      961 gaagtaaata ttcacattca aggagtgaac atagacaagg actccaggaa ggaatccggt
     1021 gacgacacac taaggttaag gtaaggtatc cggaatattc tttgggataa taaattaata
     1081 acccgccagg gtttattttt tttttaaaat gaaatataat gttaatatac attttatgaa
     1141 agcacttttt ttatgtgaaa tctgtaattc gggtattata atgggaaaac taaagataaa
     1201 aaagggtggt ggttgtcgta ttaatatcat ttttaggatt ttgctggctg tttatgcgtt
     1261 ttgtcttacg gttatttcac taataaccct cgtgctgact ttaagacctg aaatgatcga
     1321 gagggcatca aagcttcttg tgaatgatgt gttgccgaac cgtgggctta cactgctgat
     1381 gctgtttatt gaggttgctt ttttaggatt gagtcttatg ttccttttat ctggtgtgag
     1441 aagtgagaag gataaaaaat ctgttagtaa gtataataat gttggagaga taagaatatc
     1501 gttaaatgct atcgaaagta ttgcacttaa tgcttcgaaa aaggtttccg gtatcaggga
     1561 aaacaaggcc tacgttacaa aacagggaga tcatgtctcg gtttttatta agacaattgt
     1621 gatgcctgat gttaacattc cggcccttct tgaggatata caggtaaagg taaaaaaatc
     1681 tgttgaggaa tcgacaggta ttgctgtgaa tgaagtgagg gcgtcagttg aaaacatata
     1741 caccgggtat aagtccagag ttgagtgatg gaggtttata aaatggataa acaggagata
     1801 atgaaaaaaa taattggatt ttaccggttg cattatggtg gaataaacgg tgcattcata
     1861 ggtcttgttg tagcaatatg tgttttggtc ataggctttt tccagacact ttttattgcg
     1921 atttgtgttc ttgtaggtta ctatataggg aaaaagatat caaaagataa gaattatctt
     1981 agaaatctac ttgatagatt tttgccgccg ggtacatata gatagcgtta ttactttgga
     2041 actatatttg gggaggtaaa accgaatggg aagaaaggca tccagggaaa ttgcaatgaa
     2101 gcttttatac cagcttgaat ttcagaaaga ttcacgggaa gaacaaatag aagttgcact
     2161 ggagcaatac aatcttaatt caaaggacag ggaatatata atcgatataa ttaatggggt
     2221 ttataacaac aaggagagca tagacagtac cattgaaaag cattcaaggg gatggaagct
     2281 gtcaaggatt tccaaggttg acttgtccat tttaaggctc agtatatatg agatgtgcta
     2341 taggaaggat attcctctta atgtctctat aaatgaagca gttgaactgg caaagaatta
     2401 tagcggtggc gagagcggtt cctttgtaaa cggaattctg ggtaatgtag caaaaataag
     2461 gctattactg gtagatggca acgatgaata gtattaacaa tattttaacc gtatcacaag
     2521 ttaacaggta tattaaggag aatatgtcca gagatcttat tctgtcaaac ctctggataa
     2581 gaggagagat ttcgaattac aagaaccatt cttcgggaca catgtatttt actctcaaag
     2641 acgagaacag ccttataagg tgtgtaatgt tcaggacata taattcatat cttaaattta
     2701 agcccgaaaa cggtatgaag gtaataataa ggggatatat ttccgttttt gaaagagacg
     2761 ggcagtatca gctttatgca gaggaaatgc aaagcgatgg agtcggtaac cttcatattt
     2821 cttttgagca gctcaaaaaa aggctgtcgg acgaaggatt gtttgatgct gcatataaga
     2881 aaaaaatacc ctatctgcct cgttccatcg gtgtagttac ttcctccacc ggttctgtta
     2941 tccgggatat aatgaatgtt ttggacagaa ggttttataa ttcggttata aagatatttc
     3001 ctgtcaaggt tcagggtgac tctgcttgtg gagaaatatg ccatgcaatt gaaaaactca
     3061 atgagtccgg atgtgtggat gttataattc ttgcccgggg aggaggatcc ttagaagagc
     3121 tgtggccctt taatgaagaa tcggtggcaa gaagcatatt caagtccaaa atacccatta
     3181 tatctgcagt cggtcacgaa accgatta
//
LOCUS       DAOOJC010000284         3204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_23266_length_3204_cov_3.377898, whole genome shotgun sequence.
ACCESSION   DAOOJC010000284 DAOOJC010000000
VERSION     DAOOJC010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23266_length_3204_cov_3.377898"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1941
                     /gene="pknB"
                     /locus_tag="PLA01_07810"
     CDS             <1..1941
                     /gene="pknB"
                     /locus_tag="PLA01_07810"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HPD01235.1"
                     /translation="RGAEMVGQILGNRYELIEKIGGGGMALVYKAKCRLLNRFVAIKI
                     LRTEFINDEEFLKRFRIEAQAAASLSHPNIVSIYDVGQEDDIHYIVMEFVNGMTLKDY
                     LEQKGPLDWREAVNIVIQISLAIEHAHKNHIVHRDIKPHNILLTKECIVKVTDFGIAR
                     AVSSSTITMAGSTIGTVHYFSPEQARGGFIDEKSDLYSLGIVLYELLTGKLPFEGESP
                     VAVALKHIQDEPEEPISINPGIPKGVNSIVMRAIQKDQSMRYQSATELLDDLNKVLKE
                     PNNEFKKIESVKDSPTVRMPSIGKEKLTVQKDTSEKTGDKNVRKKKERMTTILAVVTS
                     VIIIGIIAAFAVKGLGPIVYSLFDKTEDFIVQDYTNQNYYEVKGRLSEYGIEVVENRK
                     NHDDIPKDIIIEQDKAAGERIKPGGYAKIVFQVSDGPLLLKIKDYRRREYREVETELV
                     QLGLKTDLIEEYNDDVAKGLVIKTDPDINEEVKPGSVIKVYRSLGPEIVTSLVPNLIG
                     KTQSEALGLLTGVNLTMGDIYPEDKTYAMDKIIKQEPEAGFEVEEGTPVKIFLEDYNP
                     DQKNVNSVIKLEDPHNYGERIKVIVNITRSDTNRTETIYSETRRKSDFPITVPIPVPN
                     GGSTLVRVYLDSSLYKEFTEIF"
     gene            1955..2839
                     /gene="rsgA"
                     /locus_tag="PLA01_07815"
     CDS             1955..2839
                     /gene="rsgA"
                     /locus_tag="PLA01_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPD01236.1"
                     /translation="MPSGVIIKGIGGFYYVKAADAVYECKAKGTFRKDSKIPLPGDNV
                     SISVVDREKRLGFIEEIYPRTLELIRPAVANVNQAALVVSVKSPLPDYYLLDKLLITV
                     MQKEISGVICINKIDLDEENKNKIAECYKCTGIKTIFISSVLNVGFDELEKVLTGKTT
                     VFAGQSGVGKSTILNRVMDSYVMETGNVSDKIDRGKHTTRHAELIELKSGGFVVDTPG
                     FSSFEITDIEPGDLQKYYPEFGEFIGKCRFAGCSHISEPGCEVKSALEKGFISTGRYE
                     RYIEFNNILKDKQRKKYS"
     gene            2952..>3204
                     /locus_tag="PLA01_07820"
     CDS             2952..>3204
                     /locus_tag="PLA01_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPD01237.1"
                     /translation="MIKIAPSILSADFSKLGEEILRIEKAGADLVHIDVMDGHFVPNI
                     TIGPPVVKSLKKVTGLLFDVHLMIENPDSYIDAFVDAGAD"
BASE COUNT         1031 a          476 c          804 g          893 t
ORIGIN      
        1 agaggtgctg aaatggtagg tcaaattcta ggtaacaggt atgaattgat tgagaaaatc
       61 ggtgggggcg gtatggccct tgtatataag gctaaatgta gattgttgaa caggtttgta
      121 gctataaaaa ttttaagaac cgaattcata aacgatgagg aatttttaaa gaggtttcgt
      181 attgaagccc aggctgcagc gagcctttcc catcccaata ttgtctcaat atatgatgtg
      241 ggacaagagg atgacataca ttatattgtc atggaatttg tgaacggaat gactttgaag
      301 gattatttgg agcaaaaggg tcctcttgac tggagggaag cggtaaatat cgtaatccaa
      361 atttccttgg caattgagca tgcacataaa aatcatattg ttcaccggga tattaaaccc
      421 cataatattc ttttgacgaa ggaatgcatt gtaaaggtaa cggattttgg aatagcaaga
      481 gccgtcagtt catcgaccat aaccatggcc ggaagtacta taggtacggt gcattatttt
      541 tcaccggagc aggcaagggg tggatttatt gatgaaaagt cggaccttta ttcattggga
      601 atagtattgt atgaactgtt gacaggaaaa cttccttttg aaggagagtc gcctgttgct
      661 gttgcattga agcatattca ggatgaacct gaggaaccta tcagcattaa tcccggaata
      721 ccaaagggtg taaacagtat tgttatgcgt gccattcaaa aggatcagag catgcggtac
      781 caatctgcta ccgagctttt ggatgatttg aacaaggttt taaaagaacc gaataatgag
      841 tttaaaaaaa ttgagtctgt caaagacagt cctacggtaa ggatgccatc gattggtaag
      901 gaaaagttga ctgtccaaaa ggatacttct gaaaaaacgg gtgataagaa cgtgaggaag
      961 aaaaaagaaa gaatgacaac aattctggcg gtggttacat cagtaatcat tattgggatt
     1021 attgctgcat tcgcggtaaa aggtctgggc ccgattgttt actccttgtt tgataagaca
     1081 gaggacttta ttgttcagga ctatacaaac cagaattatt atgaggtaaa gggcagactt
     1141 tcggagtatg ggattgaagt tgtcgaaaac agaaagaatc atgatgatat tcccaaggat
     1201 atcattattg agcaggacaa agcggcgggc gaaagaataa agcccggagg ctatgctaaa
     1261 atagtgttcc aggtaagtga cggaccgttg ttgctgaaga ttaaagatta caggagaagg
     1321 gaatacaggg aggttgaaac ggagctggtt cagctgggtc ttaaaaccga tttaattgag
     1381 gagtacaatg atgatgttgc aaagggactt gtgataaaga ctgaccccga tattaatgaa
     1441 gaagttaagc ccggtagtgt tatcaaggta tacaggagtc ttggaccgga aatagtaacc
     1501 agtcttgttc ccaatttgat tggcaaaact caaagcgagg ctttgggttt gctgaccggt
     1561 gttaatttga cgatgggtga tatatacccg gaagataaga catacgccat ggataaaata
     1621 ataaagcagg agccggaagc aggttttgaa gttgaagagg gtactcctgt gaagattttc
     1681 ctggaggatt acaatcctga ccagaagaat gtcaatagtg ttataaagct tgaggatccc
     1741 cataattacg gagagagaat aaaggtaata gtaaatatta caaggtcaga taccaacagg
     1801 acagagacca tatacagtga aacgagaagg aaaagtgact tcccgataac agttccgata
     1861 cctgttccta atggaggaag tactctggta cgcgtttatc tggacagcag tttgtataag
     1921 gaattcactg agatttttta aggaggtaga aagtttgcca tcgggtgtga taattaaagg
     1981 tattgggggg ttttattacg ttaaagcggc ggatgcagta tatgaatgca aggcaaaagg
     2041 tacgttccgt aaagactcca aaattccttt acctggtgat aatgtttcta tttctgttgt
     2101 tgaccgggaa aaaaggcttg ggtttattga agaaatctat ccgagaacac ttgagcttat
     2161 aagaccggca gttgcaaatg taaatcaggc tgcactggtt gtttcggtta aatcgcccct
     2221 gccggactat tatctattgg ataaattatt aattacagta atgcaaaaag aaatcagcgg
     2281 agtaatttgt ataaacaaga ttgaccttga cgaggaaaac aaaaataaaa tagccgaatg
     2341 ctataaatgc acaggtatta aaaccatttt tattagttcc gtattgaatg tgggttttga
     2401 tgagcttgaa aaggttctta cgggtaagac tactgtgttt gcaggacagt cgggagtcgg
     2461 aaagtccaca attctaaaca gggttatgga ttcctatgtt atggaaacag gcaatgtaag
     2521 cgataaaatt gacaggggaa agcatactac gaggcatgca gagttgatag agctaaagtc
     2581 aggcggattt gtggtggata cgccgggctt tagttctttt gagataacag atattgagcc
     2641 tggggattta cagaagtatt atccggaatt cggtgagttt attggaaaat gcagatttgc
     2701 cggatgcagt catataagcg aaccggggtg cgaagtcaag tccgctcttg aaaaaggctt
     2761 tatatctacc ggaagatatg aacggtatat tgagttcaat aacattttaa aggataagca
     2821 gcgtaagaaa tattcgtagc tgtataatct aaatagagta acgtataaat tcatcaagta
     2881 aagcattatg aaattgtaat ggttcaagct gttaaatttt gtggttcaac tgggaaggga
     2941 agtgttttgt attgattaag attgctccat ccattctttc agccgacttt tcgaagctgg
     3001 gtgaggaaat tttgcgaatt gaaaaggcag gggcagattt ggtgcatatt gatgttatgg
     3061 atggacattt tgtacctaat ataacaatag gtcctccggt tgttaagtct ttgaaaaaag
     3121 taaccgggct gttgtttgat gttcacctta tgattgaaaa tcccgatagc tacatagatg
     3181 cttttgttga tgcaggtgcc gata
//
LOCUS       DAOOJC010000285         3198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_23323_length_3198_cov_3.530703, whole genome shotgun sequence.
ACCESSION   DAOOJC010000285 DAOOJC010000000
VERSION     DAOOJC010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23323_length_3198_cov_3.530703"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..>869)
                     /gene="miaA"
                     /locus_tag="PLA01_07825"
     CDS             complement(37..>869)
                     /gene="miaA"
                     /locus_tag="PLA01_07825"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPD01238.1"
                     /translation="IYKYMDIGTAKPDEKEREGIKHYLIDEVNPDEEFSVARFKELAL
                     QYINEIIKKGKVPIVTGGTGLYINSLIYNLQFSDTICDWDLRDKLAKEAVEKGNEYIH
                     NMLKEIDPVAAQKIHKNNVKRVIRAIEVYNYTKKPISVHQEESRRNPPKQNFILIGIT
                     MDRQKLYDRINKRVDLMLENGLVKEVEKLVEMGYDKSTIAMQGLGYKEILAYLKKEIT
                     LDEAIEILKRDTRRYAKRQITWFKKLENVCWINMDEIASQEKILKNIKYHLATYGIFL
                     "
     assembly_gap    870..879
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(973..3057)
                     /gene="mutL"
                     /locus_tag="PLA01_07830"
     CDS             complement(973..3057)
                     /gene="mutL"
                     /locus_tag="PLA01_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPD01239.1"
                     /translation="MGKIVILDENTSNQIAAGEVVERPASVVKELVENSIDAGSTSIS
                     VEINNGGISLIKVVDNGAGFEEDDMEIAFERHSTSKIRKAGDLEAITTLGFRGEALAS
                     IASVSTVELTSRQEKNTYGRYVKLRGGTMVESGQVGCPVGTTFVVRELFYNTPARFKF
                     LKKDSTEAGYVSDIVGRIALGNPHISLKLVNNRSTVIHTPGNNDILSTIFSLYGKETA
                     RDCMEISYSDEKIQIKGYAGRPEIARSNRNYQSIYLNGRYIKNKVISSAIDEAYKTFL
                     LKNKHAFIVLYIELNPMLVDVNVHPTKTEVRFSNEQDIFRAVYHAVNNTLLGKSRVRS
                     AELRDNPKNYFRFEENTKSKNDYIQQKLNTSSRKNDAVEPSFKATESTLEKRDASEKE
                     MAAPFDIQKSESVPDNTRNSSNVLENNIAEDLSEDKVKSRDITDKDLSEVIVKSRDII
                     GKDLLNDDSKSRDLAGKDLPGRDSITKDSPCMDMEEKDAIEENLSGQIAEPIFQDAII
                     IGQAFSTYILLQKGENLILIDQHAAHERIMFESLKEKYSKKENLAQYLLASVVIELTN
                     REISFIEEEKDLLNKLGFNFESFGNTSIILRSVPADIDGCGIKEAFMEIIDFLMSKGS
                     KSGSIVAEEALYKIACKSAVKANKRLDEKEIKELLQKLEELENPYTCPHGRPTIIKIT
                     RYEFEKMFKRVL"
BASE COUNT          839 a          735 c          501 g         1113 t
ORIGIN      
        1 caaaggacta ttctatcaaa ccctatcgct tatattctat aagaaaatac cataagttgc
       61 aagatgatat tttatatttt ttaatatttt ttcttgactt gctatttcat ccatatttat
      121 ccaacaaacg ttttcgagct ttttaaacca ggttatctga cgttttgcat accgtctcgt
      181 atcccgcttt aaaatctcaa tggcttcatc caaagtgatc tctttcttca agtatgccag
      241 tatttcctta tagcccagtc cctgcatggc aatcgtactt ttatcatacc ccatttccac
      301 aagcttttca acttctttca ccaaaccgtt ttcgagcatc agatccaccc tcttgttaat
      361 cctgtcataa agcttttgcc tgtccatagt aattccgata agtataaaat tttgttttgg
      421 cggatttcgt ctcgactcct cctggtgaac agaaataggc tttttagtat agttgtatac
      481 ctctatcgca cgaataaccc tcttaacatt gttcttatgt attttctgtg ctgccacagg
      541 gtcaatttct ttgagcatgt tatggatgta ttcgttgcct ttttccacag cctccttcgc
      601 tagtttgtcc cttaaatccc aatcacaaat tgtatcggaa aattgaaggt tatatattag
      661 cgaatttata taaagacctg ttcctcctgt tacaattgga actttaccct tttttattat
      721 ttcattaata tattgtaagg caagctcctt aaatctggca acgctaaact cttcatccgg
      781 attaacttcg tcaataaggt agtgctttat tccttccctc tctttttcat caggcttggc
      841 agtacctata tccatatatt tgtatatctn nnnnnnnnna gcttagtttt tcccgatgcc
      901 gtcggaccga ttatcacaac tacattatcc aatttaaacc tccaatataa cagcacaaat
      961 tgtaaatatg agctatagaa cccgtttaaa catcttttca aattcgtacc gggtaatctt
     1021 tataattgtc ggccgtccgt ggggacatgt ataagggttt tcaagctctt caagcttttg
     1081 caatagctcc tttatctctt tttcgtcaag tcttttattc gctttaacgg cagacttgca
     1141 cgctattttg tataatgctt cctctgcaac aatgctgccg cttttgcttc cttttgacat
     1201 taaaaagtcg ataatttcca taaatgcttc ctttattccg catccgtcaa tatcggcggg
     1261 taccgaacgc agtataatag aagtatttcc aaagctttcg aaattaaaac ccaatttgtt
     1321 caataagtct ttttcttcct caataaagct tatttcccgg ttcgtaagct caataaccac
     1381 cgatgccagc aagtactgag ccaggttctc tttttttgag tatttttctt taaggctttc
     1441 aaacattatt ctttcatggg cggcgtgctg gtcaataaga atgagatttt cacccttttg
     1501 aagcagtatg taagtagaaa aggcctggcc gattataatg gcatcctgga aaatgggctc
     1561 tgcaatttgt cctgaaagat tctcttctat tgcatccttt tcttccatat ccatgcaagg
     1621 cgaatctttc gttatactat cccgaccagg taaatcctta cccgctaaat ccctgctttt
     1681 tgaatcatcg ttcaataaat ctttgccgat tatatctctg cttttaacta taacctccga
     1741 taaatctttg tcggttatat ctctgctttt tactttatcc tccgataaat cttcggctat
     1801 attgttttca aggacattgc tgctatttct agtattatcc ggcacagact cagatttctg
     1861 aatatcgaaa ggagcagcca tttctttttc agatgcatcc cttttttcca aagtactttc
     1921 agttgcctta aaagaaggct caacagcatc attttttctg cttgaagtat ttagcttctg
     1981 ctgtatataa tcattctttg attttgtgtt ttcctcaaac ctgaaatagt ttttcgggtt
     2041 atcacgaagc tcagcacttc gaacacggga ctttcccaaa agtgtattat tcacggcatg
     2101 gtaaacagcc ctgaatatat cctgttcatt ggaaaaacgc acctcagttt ttgtgggatg
     2161 tacatttaca tccaccagca ttggattcaa ctcaatatac agcacaataa aagcatgttt
     2221 attctttaaa agaaaggttt tatatgcctc atcaattgcc gatgatataa ccttgttctt
     2281 tatgtaacgt ccgtttaaat agatggactg gtagttcctg tttgaccttg caatctcggg
     2341 ccttccggca tatcctttga tttgtatttt ctcatcgcta taggaaatct ccatacaatc
     2401 cctggcagtt tcctttccgt agaggctgaa tatggtgctg agaatatcat tattcccagg
     2461 ggtatgaata accgtgcttc tgttgtttac aagctttaaa gatatatgcg gatttcccag
     2521 agcaattctt ccgactatat cggacacata ccctgcctct gtggaatcct tctttaaaaa
     2581 cttgaacctc gcaggggtat tgtaaaaaag ctctctcact acaaaggtag ttcccacagg
     2641 acaacccacc tgtcccgact ctaccatcgt accgcctcta agctttacat acctgccata
     2701 ggtgtttttt tcctgtcttg aagtgagttc aacggttgaa accgatgcaa tacttgcaag
     2761 agcctctcct ctaaagccaa gggtggtaat ggcctcaagg tcaccggctt ttcttatctt
     2821 actggtggaa tgcctctcaa aggcaatttc catatcatcc tcttcaaacc cggcaccgtt
     2881 gtcaaccacc tttattaagg aaatgccacc attattaatc tctaccgaaa tgctggtact
     2941 tcctgcatca atggaattct caacaagttc cttaacaact gaagcaggtc tttccaccac
     3001 ctcgccggca gctatctgat tggatgtatt ttcatcaagt atcactatct ttcccataca
     3061 ttactcctca actcaaagca tatttataac ttccggtatc agttcttaac ctttttttga
     3121 agggaataaa gcacattcaa ggcatctaac ggtgttaagg tggaaatgtc aatatccctt
     3181 atctcgttta aaacctca
//
LOCUS       DAOOJC010000286         3197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_23333_length_3197_cov_3.682050, whole genome shotgun sequence.
ACCESSION   DAOOJC010000286 DAOOJC010000000
VERSION     DAOOJC010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23333_length_3197_cov_3.682050"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="PLA01_07835"
     CDS             complement(<1..1135)
                     /locus_tag="PLA01_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPD01240.1"
                     /translation="MSQVVLMRCESYDYNTVKSAVKKGIELIGGPLKFASPQEKILLK
                     PNLLSADPPERCSTTHPSVFKAIAEVFIEAGINLTYGDSPGLHKPQTAAHKAGLEQAA
                     NELGISLADFIGGKEIFFEKGIQNKKFTLANGVLESDGIISIPKLKTHGLARITGCIK
                     NQFGCIPGPLKGEFHARIPDVIDFSKMLVDLNTYLKPRLFVMDGIMAMEGNGPRGGTP
                     KKMNVVLLSADPIALDATVCRMIGLNPEFVPTIVFGKEAGLGTYLENEIEILGDSLDS
                     FTDPSFDVRREPVRRFKAKGAIQFLRNTLVPKPYIEETKCKKCGICVNMCPVNPKAVD
                     WHNGNKKEVPPSYIYKRCIRCYCCQEICPESAINLRVPFIRRFI"
     gene            complement(1489..1650)
                     /locus_tag="PLA01_07840"
     CDS             complement(1489..1650)
                     /locus_tag="PLA01_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01241.1"
                     /translation="MAKAKNKQNKDNKNIKDASGKYEFANDQLGENMENRYEGMAKKG
                     KNSNGGKCK"
     gene            1886..3133
                     /locus_tag="PLA01_07845"
     CDS             1886..3133
                     /locus_tag="PLA01_07845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HPD01242.1"
                     /translation="MNRKILVQIQCFVAVLVIVFSCKDFAFADREPLQIAAPSAILVE
                     SQRGQELYEKDSKKKLHISSANKIMTALITLENMKDQLNTKVTISKNAVSVEGATLNL
                     EVGEKYSVEDLLYSVILDSVNDSAIALAEYIGGDEKGFVELMNKKAIELKMSDTHFTN
                     ATGLYNEQQYTSAYDIALLIRYAVTKYSKFQKIFSSKAWPWSNNGKVLINSNELFWSY
                     EGVDGGKTGYNDIERQTAITTVTRNGQRLICIVLDSPKDSVYDDSIKLLDYGFENFRT
                     GVLVSNGETIKSIPVGDEMLDLIALADYYYTYPIGEDYIKDFDISLRSNLEPPIRKNE
                     ILGTAKYTLDDGTVIQVNLYPGSELYSTMSWFSSLASKFSEYRDIVILVLILLAIEVF
                     LLIIYTIRLIKKVFTKLLGRSKN"
BASE COUNT          976 a          551 c          621 g         1049 t
ORIGIN      
        1 aaataaacct gcgtataaaa ggaactctaa gatttatggc actttcagga caaatctcct
       61 ggcaacagta gcaccttatg catcttttgt aaatatatga aggaggtact tcctttttat
      121 tgccattgtg ccaatccact gctttgggat taacagggca catgtttacg catatcccgc
      181 attttttgca tttggtttct tcaatatatg gtttgggcac aagggtgttt ctcagaaact
      241 ggattgcacc tttagctttg aaccgcctta caggttctct tcttacgtcg aagctggggt
      301 ctgtaaagct gtccaggcta tctcccagta tttctatttc attttcaaga tacgtaccga
      361 gacctgcttc ttttccaaat acgattgtcg gaacaaactc cggattcagc ccaatcattc
      421 tgcagacagt ggcatcgagt gcaatagggt ccgctgataa taaaacgaca ttcatctttt
      481 tgggagttcc acccctgggt ccattgcctt ccattgccat tataccgtcc ataacaaaaa
      541 gtcttggctt gagataggtg tttagatcca caagcatttt tgaaaagtca atgacatcgg
      601 gaatgcgggc atggaattct ccctttaaag gtccgggtat gcatccgaac tgattcttta
      661 tgcatcctgt tattcttgcc aagccgtgag tttttagctt gggtatgctt attatgccgt
      721 cactttcaag aacaccgttg gctaatgtga attttttatt ttgtattccc ttttcaaaga
      781 aaatttcttt acctccgata aaatcggcaa ggctaatgcc aagctcgttt gctgcttgtt
      841 caagaccggc tttgtgggca gcggtttgag gcttgtgaag ccccggtgaa tcaccatagg
      901 ttaaattaat acctgcctca atgaaaactt cagcgatggc tttaaatact gaaggatgtg
      961 ttgtactgca tctttcagga gggtccgctg ataagaggtt gggctttaag aggatttttt
     1021 cttgcggcga tgcaaatttg agtggaccgc caattagttc aatacccttt tttacagctg
     1081 atttaacagt attataatca tagctttcac atctcattaa aactacttgt gacatttatt
     1141 atttatctcc tttgcttgtc tgtttatatt tattagactt ttcaacctgc aatatgaagt
     1201 taattttact aaatactaat gggatggata gaattttcgg cgatgccgta atgttcagta
     1261 tatagtttat gctagcatat tacaaagaaa aatggaagga taaatttata aggaagaaca
     1321 aaaggtaaag aaaaatattc cggaaactta agttcattcc atgattttct ttactttacc
     1381 tccatcagca gtgagtacat aaagattaat gcataatcct atacgaaaaa taaaggggct
     1441 taatgcccct caatatttaa gataaatatc aaccggcatt aaaaaattct atttgcattt
     1501 accgccgttg ctgtttttcc ctttcttagc cattccctcg tatctgtttt ccatattttc
     1561 tcccaattgg tcatttgcaa attcatattt gccggatgca tctttgatgt ttttattatc
     1621 tttattctgt ttattctttg ctttagccat aatatcacct ccaattttat ttttcctgaa
     1681 ttactgaaag tcatgcatgg agggaaaaga ataataaata atactcatag atccgaaaaa
     1741 taatgctcag ttggataaaa tattcataat tttaagttgt attacagaaa ttaaagtact
     1801 ataatattgt caaatagcat taacaaaagg aagactattg gtattactga ataacatttc
     1861 atttgtagat tcggatgggg tagatatgaa tagaaaaatc ttggttcaaa tacaatgttt
     1921 tgttgctgtt ttggttattg tgttttcttg caaagatttc gcttttgcag atagggaacc
     1981 tttgcagata gcggctcctt ctgcaatctt agttgaatca caaagaggac aggaacttta
     2041 cgagaaggat tcaaagaaga agcttcatat ttccagtgcc aataaaataa tgactgcttt
     2101 aattaccctt gaaaatatga aagaccagtt gaatacaaag gttactatca gtaagaatgc
     2161 tgtttcggtt gagggagcga cattaaatct ggaagttggc gaaaagtatt cggttgagga
     2221 tcttttgtat tcggttatat tagattcggt caatgatagt gccattgcct tggcagagta
     2281 tataggcggg gatgaaaagg gatttgttga acttatgaat aagaaggcaa tagaattgaa
     2341 aatgtctgat acccatttta caaatgccac aggcttatat aacgaacaac agtatacatc
     2401 agcatacgat attgctcttt taataagata tgctgtaaca aaatacagta aatttcagaa
     2461 aattttttct tcaaaggcat ggccatggtc taataatgga aaggttctga taaacagtaa
     2521 tgaacttttc tggagttatg agggggttga cggcggtaag acgggataca atgatataga
     2581 acgtcagact gcgataacta cagtcacaag aaacggacag agattaatat gcatagtgct
     2641 ggattctccc aaagacagtg tttatgatga ctccataaag ttgttggatt acggatttga
     2701 aaatttccgg acaggggttt tggtttcaaa tggagagacc ataaaaagca ttcccgtagg
     2761 tgatgagatg cttgacctta tagctttagc cgattattat tatacatatc ccattggcga
     2821 ggactatatt aaagattttg acatcagttt acggagtaat ttagagcctc cgataaggaa
     2881 gaatgaaatt cttggaactg caaagtatac actggatgac ggaactgtta ttcaagtaaa
     2941 cctttatccc ggatctgaat tgtattcaac gatgagttgg ttttcatcat tagcatcaaa
     3001 attcagtgag tacagagata tagtaatact ggttttgatt cttttggcaa tagaggtatt
     3061 tcttttgatc atttatacaa tcagacttat aaaaaaagta ttcactaaac tgcttggcag
     3121 gtcaaaaaac tgagctgtac acacagggct atcgcatgaa gcaatataat tccaaaagta
     3181 aatcctgtat attctta
//
LOCUS       DAOOJC010000287         3184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_23462_length_3184_cov_4.135507, whole genome shotgun sequence.
ACCESSION   DAOOJC010000287 DAOOJC010000000
VERSION     DAOOJC010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23462_length_3184_cov_4.135507"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..521
                     /locus_tag="PLA01_07850"
     CDS             180..521
                     /locus_tag="PLA01_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HPD01243.1"
                     /translation="MAFFDREIDITRNIKIIEWLKSELLTDIANLFKALVNGVREEVH
                     ESVSETLSNIILISYMLGRRLGISYNSIELKMHNKIRLGIIEEHDVEKYYGDLTELAK
                     HLKSSRIESKV"
     gene            613..2637
                     /locus_tag="PLA01_07855"
     CDS             613..2637
                     /locus_tag="PLA01_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPD01244.1"
                     /translation="MKRKKISGIFLPKASFYLFVIFILLILVAVLEFVAAIPGFILFI
                     VLLWYNFRSNYIRHRELTKYIENLNFNIDTASKDTLLNFPMPLVVIELDGTIIWYNSS
                     FKRLFDGEELLEKTIHSFVKGMNPDNLSDEGAMSFTKDILINGRSYCALGNFAKVDDK
                     INEDGFIIMMYLIDNTELVEIKKRYDDEKTTVGIIVIDNYDDLMQSIEDARSAQILAE
                     IDSKLTRWMGYTNGIIKKFERDKYLFVFDHRYLKDFVEKKFDILDSVKEINVGNKIPV
                     TLSMGFGVGTHSLQEKFRFANASLDIALGRGGDQVVVKNGEDIMFFGGKTRELEKRTR
                     VKARVIAYALRELIEQASDVLIMGHDNCDIDCLGSALGIYRIAKNRNKEAFIVLNNSN
                     PTIDCLVSRIEGSGEYKSLFIASSEAIDRITKKTLLVIVDTHRPSFTEAPDLLKYTDQ
                     IVVIDHHRRGADYLQEAVLTYQETYASSTCELVTEILQYVEDKIKLKPIEAESLYAGI
                     VIDTKNFTFKTGVRTFEAAAFLRRQGVDTVQVKQLFQNDINTYISIASVVRDAQVIRD
                     NIAISVCPPNMKNAALVAAQAADQLISLAGLNAAFVFSYINNGINNGIAISGRSLGDV
                     NVQVVLEKLGGGGHMTVAGAQLQGVSIEEARKSLLTALDEYFDEISKQNG"
     gene            2698..3147
                     /gene="rplI"
                     /locus_tag="PLA01_07860"
     CDS             2698..3147
                     /gene="rplI"
                     /locus_tag="PLA01_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPD01245.1"
                     /translation="MKVILKEDVKGVGSKESLVNVSDGYARNYLIPRGLAVEATSVNI
                     NVMKTKKEAEKSKKDRELAQAKELAEKLKNVEVVIKAKAGENGKLFGSITGKDISDYL
                     KKNHKFDIDKKKIALSDSIKALGTYEAEVKLYSGVSTKLTVKIEQGE"
BASE COUNT         1039 a          496 c          783 g          866 t
ORIGIN      
        1 aataagtttt tttaggctca ggagaactga attgttggga aatgccacac cggttattat
       61 tttagtgata taatatatgg cacatgtatt taccatgcat cggtccattg gacaaaaacc
      121 caaattgtga gagaccaacc gggttcaata ctaaataata tttgtgctgg agtggttata
      181 tggctttttt tgacagggaa attgatatta caaggaacat taaaattata gagtggctca
      241 aaagcgagct tcttaccgac atagcgaacc tttttaaagc tttggtaaac ggggtcaggg
      301 aagaggtgca cgaatcggtt tcggaaaccc tctccaatat tattttaata tcgtatatgt
      361 tgggaagacg tttgggcatt agctataatt ccattgaatt aaagatgcac aacaagatta
      421 ggcttggtat aatagaggag catgatgtgg aaaagtatta tggggatttg accgaattgg
      481 ccaagcatct gaagtcttca aggattgaaa gcaaggttta atgattggca gaaacgaaag
      541 aagtgtggtg gcacaagtca ccgctcggag ttctttacgg gattttttaa ggccataaat
      601 gggagaagtg tgatgaaaag aaaaaagatt tccggaatat tcttgccaaa agcaagtttt
      661 tatctgtttg taatatttat tctcttaata ctggttgccg ttcttgaatt tgttgcggca
      721 atacccggtt ttatattgtt tatagttctt ttgtggtata acttcaggtc gaattatata
      781 aggcaccggg aacttaccaa gtacattgaa aatttgaatt ttaacattga tacggcttca
      841 aaggacacgt tgcttaattt tcccatgcca ttagtggtta ttgaactgga tggaactata
      901 atctggtata attcgtcttt taaaaggctg tttgacgggg aagagcttct ggaaaaaaca
      961 atacattctt ttgtaaaggg tatgaatccc gacaatctgt ccgatgaagg agcaatgagc
     1021 tttacaaagg acatattaat aaacggcaga agctactgcg cccttggaaa ctttgccaag
     1081 gtggacgata agataaatga agacggcttt attataatga tgtacctgat tgacaacact
     1141 gaacttgttg agatcaaaaa aaggtatgac gatgaaaaaa caacagtggg aattatagtg
     1201 atagacaatt atgacgattt gatgcagagt atagaggatg cacgaagtgc acagatcctg
     1261 gcggaaatag attcaaaatt gacccggtgg atggggtata caaatggaat aattaagaaa
     1321 tttgaacggg acaaatacct gtttgttttc gatcacagat atcttaagga ctttgttgag
     1381 aaaaagttcg atatcctcga ctccgttaag gaaataaatg tgggaaataa aatccctgtg
     1441 accctgagta tggggtttgg tgtgggaaca cattccctgc aggaaaaatt ccggtttgca
     1501 aatgcttctt tggacattgc tttaggaaga ggcggcgatc aggtggttgt aaagaacgga
     1561 gaggacataa tgttttttgg cggaaagacc agggaattgg agaagaggac aagagtcaag
     1621 gcaagggtta ttgcctatgc attgagggag cttattgaac aggcatccga cgtattgatt
     1681 atggggcatg ataattgtga cattgattgt ctcggttcag ccctcggaat ttacaggatt
     1741 gcaaagaaca gaaacaagga ggcgtttatt gtcctgaaca attccaatcc tacaattgac
     1801 tgcttggtat cgaggataga aggaagcggt gaatataaaa gtctttttat tgcaagcagt
     1861 gaggcaattg acagaataac caaaaagacg cttttggtta ttgtggatac ccacagaccg
     1921 agctttaccg aggcaccgga tcttttgaag tacaccgatc agatagttgt tatagaccat
     1981 cacagaaggg gtgcagatta tctgcaggaa gcggttctta cttatcagga aacgtatgcc
     2041 tcatccactt gcgagctggt aacagagata cttcagtatg tagaggataa aataaagctc
     2101 aagcctattg aggccgagag cttgtatgcg ggaattgtta tagataccaa aaactttacc
     2161 tttaagaccg gggttcgtac ctttgaagcc gcagccttct taagaaggca gggcgtggac
     2221 actgttcagg taaagcaatt gttccagaat gatatcaata cctatataag catagcgagt
     2281 gttgtaaggg atgcacaggt tataagggat aacatagcca tatctgtctg tcctccaaat
     2341 atgaaaaacg ccgctttggt ggctgcacag gcagcagacc agcttatcag ccttgccggg
     2401 ctgaatgctg cttttgtatt tagttatatt aataacggca taaataacgg catagccata
     2461 agcggaaggt ctttaggaga tgtaaatgtc caggtggtct tggaaaagct cggcggggga
     2521 ggacatatga ctgtcgctgg agcacaactt caaggcgtat cgatagagga agccagaaaa
     2581 agccttttga cagcactgga tgaatatttt gatgaaatca gcaagcaaaa tggttagaat
     2641 tctgttatta tattatatat aaattaagga ttatatataa attaaggaga gatacatatg
     2701 aaggtcattt taaaagagga tgtcaaaggt gtgggcagta aggaaagcct tgtaaatgtg
     2761 agtgacgggt atgccagaaa ttatttgatt ccaagaggat tggctgttga ggctacatct
     2821 gtgaacataa atgttatgaa aacaaaaaaa gaggccgaaa aatcgaaaaa ggaccgtgaa
     2881 ctggcacagg caaaagaact tgcagaaaag cttaaaaacg ttgaggttgt gattaaggcc
     2941 aaggccggag aaaacgggaa gcttttcggc tcaataacgg gcaaggatat atccgattac
     3001 ctgaaaaaga accataaatt tgatatagac aagaaaaaaa ttgcactttc agattccatt
     3061 aaggcattgg gaacctatga agcggaagtg aagctttatt cgggagtgag cacaaagctg
     3121 accgtaaaaa ttgaacaggg cgagtgatag attatggatc tgggttcgtt gggaaggata
     3181 cccc
//
LOCUS       DAOOJC010000288         3176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_23535_length_3176_cov_3.448574, whole genome shotgun sequence.
ACCESSION   DAOOJC010000288 DAOOJC010000000
VERSION     DAOOJC010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23535_length_3176_cov_3.448574"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLA01_07865"
     CDS             complement(<1..189)
                     /locus_tag="PLA01_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01246.1"
                     /translation="MNYFENTLNSESETIKKLSTGGILSIEERALLLNLEGDKPAELI
                     AEIEKLLTDSDDDMFITTA"
     gene            complement(200..550)
                     /locus_tag="PLA01_07870"
     CDS             complement(200..550)
                     /locus_tag="PLA01_07870"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01247.1"
                     /translation="MSNEDMPIEEVSESTLDDIRKDKLKELDTDGDGISDYDEMYYYN
                     TNPYLSDTDGDGIPDGREIALGMNPLERNQKTNKDQQTGGIAEKIAIAKQKAAEQGHT
                     ISHNEKVNQKDFER"
     gene            complement(553..840)
                     /locus_tag="PLA01_07875"
     CDS             complement(553..840)
                     /locus_tag="PLA01_07875"
                     /inference="COORDINATES: protein motif:HMM:NF040423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPD01248.1"
                     /translation="MSIDKDFSELYTFSEATKMYGLTDGSTLRKAVQRGRFKEGEIKK
                     VGTTWVVTKAAMDRLYGNKKDITSRIKDAKKKAAEQSTPQKSKAKNAQKEL"
     gene            complement(881..1486)
                     /locus_tag="PLA01_07880"
     CDS             complement(881..1486)
                     /locus_tag="PLA01_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01249.1"
                     /translation="MDRNSILKEAIRIEIELSQLKEPNSPSKTHYMVKLSNDFIKAAG
                     SDDTNSLLNSIKVKDISLSTLKDEKGIFAFTSKKHEFPSYEIEGSPNTTVNVLRQGRE
                     YALLQRSIPKENNPLNIKDPTPYIVAQNFNAKDGKCNWGQGHYCGSLDSGCSLYSEKE
                     TSSIGNKSIKERVVEAQEKANSQHNREIGKSKFDRTKENSI"
     gene            complement(1489..>3176)
                     /locus_tag="PLA01_07885"
     CDS             complement(1489..>3176)
                     /locus_tag="PLA01_07885"
                     /inference="COORDINATES: protein motif:HMM:NF037425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01250.1"
                     /translation="ESKPYLEAWNKGWHDMNLINEPVQPMPDTLKVSDMMKYGYEYVK
                     GSEMLPLGEDKALELFGKDDPVYLLHKDNTESIPQSIDEISEHFSKGGIAGIKKDDWM
                     NIVEFKEISSKASNNLEQAMNELQGSDKKPLLDMPVVTVLWSEHTAFKENEKYSLAIF
                     DEKIKRYDRQVFELKKEAEAKGDYYPYFKTKFQIEFPMDGENHTYTGRQDIGDGDGGV
                     IDHIRLVANHELKDIEEFKTGDEKSKEKMQWILDTFVPYLENVKEIEMTQDKISQLKS
                     SVDMLKAEKELTVKELSKDDNYKFFYAGSGGQDKEAIQFQKETGWKRIWGSDTQGLNG
                     DTWIVYRSVEDLPKWLQEYAAAQEKLGNIETVPSENTDKSSTKDNTGVNKLREFLINL
                     QNDEYSGKQDYFIFKKDNGDYFFVTDACNQFNHVAIGTLTADGKGDGSGKLESISKKA
                     AHYINGVQYDPVERLCSMVEGMKYVGTVRDERVLVESKVGYNRVKPTCEWAYALEQYK
                     QSLVKDKEIAQKGKRSTSKSSKGIADRMAAAKDKAKKQSKTTTKSKKERLEER"
BASE COUNT          808 a          727 c          477 g         1164 t
ORIGIN      
        1 ggctgttgta ataaacatat catcatcact atctgtaaga agtttttcta tctctgctat
       61 cagctctgcc ggtttgtcac cctctaagtt caaaagcaaa gccctttctt cgatacttaa
      121 tatgccgccg gttgaaagct tcttgattgt ttcactctct gaatttaaag tattctcaaa
      181 ataattcata accgccctcc tacctctcaa aatctttttg atttaccttt tcattatggg
      241 aaattgtatg cccctgctcc gctgcctttt gtttagcaat agcaattttt tcagctatac
      301 cgccggtctg ttggtcttta ttggtctttt gattcctttc cagaggattc atacctaaag
      361 cgatctctct cccatcgggt ataccgtcac cgtccgtatc actgagataa ggattagtat
      421 tatagtaata catttcgtca taatcgctta taccgtcacc atctgtatca agctctttga
      481 gtttatcttt acgtatgtca tctaaggttg attcagaaac ttcctctatt ggcatatctt
      541 cattactcat aattacaact ccttttgagc atttttagcc ttactttttt ggggggtact
      601 ctgttcggca gctttctttt tagcgtcttt tattctactg gtaatatcct ttttgtttcc
      661 gtaaagccta tccattgcag cctttgtcac tacccatgta gtacctactt tttttatttc
      721 cccttctttg aaccttcccc tttgaaccgc ctttcttaat gtgctgccat ccgttaaccc
      781 atacattttt gttgcttctg aaaacgtata taactctgaa aaatctttat ctatactcat
      841 aatcagacca ccttacagtt aaaattgtta ccacccctca ttatatactg ttttccttag
      901 ttctatcaaa ttttgatttc cctatttccc tgttatgctg tgaattagct ttctcttggg
      961 cttcaacaac tctttccttg atacttttgt ttccgattga ggaagtttcc ttttcagagt
     1021 acaaggaaca gccggaatca agactaccgc aataatgccc ctgcccccaa ttacatttcc
     1081 cgtcctttgc attaaaattc tgtgcgacta tgtaaggtgt ggggtcttta atgttaagag
     1141 gattattttc tttgggtatg cttctttgca ataaggcata ctcccttcct tgacgcagaa
     1201 catttacggt agtgttgggg gaaccctcta tttcataact cggaaactca tgtttcttac
     1261 tggtaaaagc aaatataccc ttttcatcct tgagagtgga taaagatata tcctttacct
     1321 ttatactgtt caataaagaa ttggtatcat cagaaccggc agccttgata aaatcattag
     1381 aaagcttcac catataatgt gttttagaag gactattcgg ctctttaagc tgacttaatt
     1441 caatctcaat tcttatagct tccttcaaaa tcgaatttct atccatgatt atctttcctc
     1501 caatctttct ttcttacttt ttgtagtagt cttactctgt tttttagcct tatccttcgc
     1561 tgcagccatc ctgtcagcaa tacccttact gctttttgaa gtagaacgct tgcctttttg
     1621 agctatttcc ttatctttga ctaatgactg tttatattgc tctaaagcat aagcccattc
     1681 gcaagtaggt ttcactctgt tataaccaac ttttgattca acaagtaccc tctcatcacg
     1741 aacagtaccc acgtatttca taccttcaac catactgcat aaacgttcta cagggtcata
     1801 ctgaacaccg ttaatgtaat gtgctgcctt tttgctgata ctttcaagtt taccgctgcc
     1861 gtcacctttt ccatctgcgg taagtgtgcc aattgcaaca tggttaaact ggttacatgc
     1921 gtctgtaaca aagaaataat ctccgttgtc tttcttaaaa atgaagtagt cctgtttacc
     1981 gctatattca tcattctgta agttaataag aaattccctt agtttattta cgccagtgtt
     2041 atcctttgta gatgatttat ctgtattctc cgaaggtaca gtttcgatat tacctaattt
     2101 ttcttgtgcg gctgcgtact cttgtaacca cttaggtaaa tcctcaacag acctgtaaac
     2161 tatccaagta tcaccattta gcccctgtgt atcagaaccc catatccttt tccaaccggt
     2221 ttctttttga aactgaatag cttctttatc ttgaccgccg gaaccggcat aaaaaaactt
     2281 gtagttatca tctttagata actcttttac tgtcagctcc ttttcagctt tcaacatatc
     2341 tacagaagat ttaagctgtg atattttatc ttgtgtcatc tcaatttctt ttacattttc
     2401 aaggtatgga acaaaagtat caagaatcca ctgcattttc tctttacttt tttcatcacc
     2461 tgttttaaat tcttcaatat ctttaagctc atggtttgcg acaagcctta tatggtcaat
     2521 aacccctccg tcaccatccc ctatatcctg tctgccggtg taggtatggt tttcaccatc
     2581 cataggaaat tcgatttgaa atttagtttt gaaataaggg tagtaatcgc ctttggcttc
     2641 tgcttccttc tttaattcaa aaacttgccg gtcgtatcgt ttgatttttt catcaaaaat
     2701 ggcaagggaa tatttttcgt tctccttaaa ggctgtatgc tcactccaaa gaacagtaac
     2761 aaccggcata tcaagcagcg gctttttgtc ggaaccttga agctcattca tggcttgttc
     2821 cagattatta cttgctttag agctgatttc tttaaactca acaatattca tccagtcatc
     2881 tttctttata ccggcaatac cacccttaga aaaatgctcc gaaatttcat ctatgctttg
     2941 gggtatggat tctgtattat ctttatgaag cagataaaca ggatcatcct tgccgaaaag
     3001 ctccaaagct ttatcttcac ctaaaggcag catttcagaa cccttaacat actcatagcc
     3061 gtacttcatc atgtcagata ccttcaatgt gtcgggcatt ggctgaaccg gctcattaat
     3121 caagttcata tcatgccaac ctttattcca tgcttcaaga taaggtttag attcgc
//
LOCUS       DAOOJC010000289         3174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_23560_length_3174_cov_3.936518, whole genome shotgun sequence.
ACCESSION   DAOOJC010000289 DAOOJC010000000
VERSION     DAOOJC010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23560_length_3174_cov_3.936518"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2918
                     /locus_tag="PLA01_07890"
     CDS             <1..2918
                     /locus_tag="PLA01_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HPD01251.1"
                     /translation="ADVWLDFLNKNDISWCNWSLTNKNETSGAFTPFELNKTEATDLD
                     PGEDQVWDLKELSVSGEYARARIKGIPYEPIDRTIREDYTTVVWDFDDGTVQGFGING
                     DSPIQEGIELTNSGNALKISGLDASNDISDGNYWANLRISADGTSARPDIFGAEKLTM
                     DVIVEEPTTVSIAAIPQSSTNGWSNPLKACKVNAEDFIEQEDGTYKALLTITTADSPN
                     FDAIAKDEQDSIMTNIIMFIGTENADVIYLDNISVSGNRAVVEEPVVHDPLGTPTFPS
                     DFEDDTRQGWNWDVASGVKSALTIEEANGSKAISWEVAYPEVKPSDGWASAPRIMLAN
                     INTTRKDNKYLTFDFYLEPVRANWGVLCINLAFAPPSLGYWAQASKTFNIPLDSLSDM
                     EKTEDGLYLFKVSFDLDDLMDDKVFESNTLLRDITIVVADVESDYAGKMYLDNIKFEP
                     EPASYLISIDETSHGSIVASSVYAKAGSVVDLTITPDSGYRLKSGSLKYNDGESYTKI
                     SGTSFIMPESNITISPVFEKKSSGSSSKGSGSSSNTVPDVSPTPTPTATPDSTTIPNT
                     PIPGVSGDKENTVQAVVYAGAETDSDGKAIASIDIEKITGAIDKALEEAAEQEKGLSS
                     TVKINAEAAKDAKSVELSIAKDGIDSLLEKGFENIIISTSLATIILDQKALSEISSSA
                     TEDVKITVSSVGVENLSDEAKNIIGDRPVLSFSVTSGGETMADFEGNITIEIPYTLKD
                     GEDAAAIVLYSVNANGELEIVKDCIYNKDTKSFTIKDAKLSQYAVGYNKIEFSDVSGW
                     YEESVNFLAARNIIKGKEKGLFAPNANITRAEFVQILANLAGADLSKYTTSFFDDVNT
                     DDWFFGAVQWAYESGIAYGSNGKFNPNANITRQDMAVMLNQFNEKVAGYTLEKVNEPV
                     VFDDDSEIAEYAKAAVAAMQEAGIISGKGNNMFDPKANATRAEASSMIALLMQKAAK"
BASE COUNT          999 a          674 c          692 g          809 t
ORIGIN      
        1 aagccgatgt gtggcttgat ttcctgaata aaaacgatat cagttggtgt aactggtcat
       61 taaccaataa aaacgaaact tccggtgcct tcacaccgtt tgaactcaat aagactgagg
      121 caaccgattt agatcccgga gaagatcagg tatgggatct taaagagcta agtgtttccg
      181 gtgaatatgc ccgggcaaga ataaagggta ttccttatga gcccattgac cgcacaataa
      241 gagaagatta tacaacagta gtttgggatt ttgatgacgg aacggttcaa ggattcggca
      301 taaatggcga cagccctata caagaaggaa tagaactgac aaattcaggt aatgccctta
      361 aaatatcagg ccttgatgcc agcaacgata tttccgacgg caactactgg gcaaacctcc
      421 gtatctccgc agacggcaca agtgcccgcc cggatatttt cggagccgag aaactgacaa
      481 tggatgttat tgtggaagaa cccacaaccg tatccatagc agccattccc caaagctcaa
      541 ccaatggctg gagcaatcct ttgaaagcct gtaaagtaaa tgcagaagat tttatcgaac
      601 aggaagacgg tacatataaa gcactattaa ccattacaac agcggattcg ccgaactttg
      661 atgcaattgc aaaggacgaa caggacagta taatgaccaa tataattatg ttcatcggta
      721 cagaaaatgc tgatgtcata tacctggaca atatttccgt atccgggaac cgtgcagttg
      781 ttgaagaacc ggttgtgcat gatcctttgg gaacaccgac atttccttct gatttcgaag
      841 acgatacccg ccagggatgg aactgggatg ttgcctccgg ggttaaaagt gcattgacca
      901 tcgaagaggc aaacggctca aaggccatct cctgggaagt agcttatccc gaggttaagc
      961 ctagtgacgg atgggcttcc gctccccgta tcatgctggc aaatattaat acaacccgta
     1021 aagataataa atatcttacc tttgattttt acttggaacc tgttcgtgca aactggggag
     1081 ttctttgcat taatctcgcc tttgcaccac ctagtctcgg ttattgggca caggcatcga
     1141 aaaccttcaa tattcctttg gattcattat ccgatatgga aaaaaccgaa gacggactct
     1201 atctcttcaa ggtcagcttc gatttggacg atttaatgga tgacaaggta tttgaatcca
     1261 atactctttt aagagatatc accattgttg tagccgacgt agaaagtgat tatgcaggaa
     1321 aaatgtatct ggacaacata aagtttgaac ccgaaccggc atcttatctg attagtattg
     1381 atgagacttc ccacggaagt attgttgcaa gttctgtgta tgcaaaagca ggctcggtcg
     1441 tagaccttac aataacccca gattccggtt atcgtctcaa atccggctcc cttaagtata
     1501 atgacggaga atcgtatacg aaaataagcg gaacaagctt catcatgccg gaatccaaca
     1561 taaccatatc accggttttc gaaaagaaat catcaggcag tagcagcaaa ggttcgggat
     1621 catcctcgaa tacagtgccg gatgtgtcac ccacacctac tccaactgca acacctgatt
     1681 ctactactat tcctaatact cctatcccag gtgtttcagg agataaggaa aacacagtac
     1741 aggccgttgt ctatgctggt gctgaaaccg acagcgatgg aaaagccata gcttcaattg
     1801 atattgaaaa aataaccggt gcaatcgaca aggctttgga agaagcagca gaacaagaaa
     1861 agggactgtc ctccacagtt aaaatcaatg cagaagcagc aaaggatgca aaatccgttg
     1921 aattaagcat tgcaaaagat ggtatcgatt ctcttttgga aaagggattt gagaatataa
     1981 taatatctac gtcactggca accatcatct tggatcaaaa agctttatcc gaaatttctt
     2041 cttccgcaac agaggacgtt aagattacag tttcttctgt cggcgtggag aatctttccg
     2101 acgaggcaaa gaatataatc ggtgacagac ccgttcttag cttcagcgta acgagtggcg
     2161 gcgagaccat ggctgatttt gagggaaata taacaataga aattccttat acccttaaag
     2221 atggtgaaga tgcagctgct atagttcttt attccgtcaa tgccaatggc gaacttgaaa
     2281 ttgttaagga ttgcatatac aacaaagaca caaaaagctt tacaattaaa gatgctaagc
     2341 tttcgcagta cgcagttggt tacaacaaaa tcgaattttc cgatgtatcc ggttggtacg
     2401 aggaatcagt aaacttcctc gccgcaagga atataataaa gggcaaggaa aaggggttat
     2461 ttgctccaaa tgcaaacatc acaagagcag agtttgttca gatactcgcc aatttggcag
     2521 gagctgacct cagcaagtat acaacctcct tctttgatga tgtaaatacc gacgactggt
     2581 tcttcggtgc cgtgcaatgg gcatacgaaa gcggtattgc ctatggctcc aatggcaaat
     2641 tcaatcccaa tgccaatata acccgtcagg acatggcagt tatgctcaat cagtttaatg
     2701 aaaaggtagc aggctatacc ttggaaaagg taaacgagcc tgttgtattc gatgatgaca
     2761 gcgaaatagc agaatatgca aaagcagcgg tagcagctat gcaagaggct ggaatcatat
     2821 ccggaaaggg caataacatg ttcgatccga aggccaatgc aacaagggct gaagcatcat
     2881 ccatgattgc tttacttatg caaaaagcag ctaaataatg tgtaaataga aataagcggc
     2941 gggtttgttg taatcctgcc gcttgctttt tatctcgtgt actattgggt gtgcaacaca
     3001 acattgatac tttctgccca cagacgatta tataaatttg caagccttat atggagataa
     3061 ttcacctttc cttctttcta ttaccctctt caagtatcca caactaaaag ggtattatta
     3121 gtcgagtaaa cccggggact ttcaccccga gcttctcaca gaaccgtacg tgat
//
LOCUS       DAOOJC010000290         3159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_23679_length_3159_cov_2.284149, whole genome shotgun sequence.
ACCESSION   DAOOJC010000290 DAOOJC010000000
VERSION     DAOOJC010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23679_length_3159_cov_2.284149"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PLA01_07895"
     CDS             complement(<1..780)
                     /locus_tag="PLA01_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD01252.1"
                     /translation="MPKENEAQYVLELRNIEKEYSGNRVLNGINLKLNPGEIHALVGE
                     NGAGKSTLMNIIFGMPVIQNTGGYKGEILIDGEVIEIKTPNEAIRFGIGMVHQEFMLM
                     PGFTITENIKLNREILKDNTVSKVLGPLLKTLDIPAMRRDANKALKELGMTVDDWLPV
                     AGLPVGYMQFIEIAREIDKENMKVLVFDEPTAVLAESEAEKLLEAMKNLANKGISIIF
                     ITHRLDEVIAVADNITVLRDGEKVGEMTKEEATLEKIAQMMV"
     gene            complement(905..2068)
                     /locus_tag="PLA01_07900"
     CDS             complement(905..2068)
                     /locus_tag="PLA01_07900"
                     /inference="COORDINATES: protein motif:HMM:NF024095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HPD01253.1"
                     /translation="MKKVLALVLALVMAFSAVACAKPAEPAAAEPAGEAAAPAAEAAK
                     AKIAIYTGTVSQNEEEFRSMEAAAKKYPGMIVSQTYPDAFTKEQETLISNAVGLVSDP
                     DVKVLIMNQSVQGAAAAFDKVREVRPDVLILAYQPGETDVIASKCDVAVTADEAAQGY
                     MMPEQAKKMGAKTFVHYSFPRHMSYPLIVQRREIMVKECERLGIKFVDATAPDPTGDA
                     GVPGAQQFILEDIPRKVAEYGKDTNFFSTNCAMQEPLLKASIEQGAIVAQQCCPSPFH
                     GYPGALGVSLEGHSGDSEYMVEQIKTKLAEKGVSGRFSSYAFPISMVSTAALVEYGIM
                     YINGELKAKNDPEALKAAYAKVSDGYTIHFDNYKLNDVVQENFYMVLADYITF"
     gene            complement(2299..2712)
                     /locus_tag="PLA01_07905"
     CDS             complement(2299..2712)
                     /locus_tag="PLA01_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01254.1"
                     /translation="MDNAISILVLTVDERQEIAEKFIAAGATDFAVKPIRSPDLISRI
                     KMNLRINEVQQKYIAEKKQVYIEKGISPATLSIICDYLKEHEAGLTIEDIANGLTLAY
                     QTVHRYVSYLLDEKKIEIIPIYGQLGRPKNKYKLR"
     gene            complement(2705..2932)
                     /locus_tag="PLA01_07910"
     CDS             complement(2705..2932)
                     /locus_tag="PLA01_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD01255.1"
                     /translation="MTINDKLEIVIIDDESDILYTIKEICAFGGWKAATAHNGREGYE
                     LCRVHKPNLAIVDYHMPDWDGLTTVKNPAVG"
     gene            complement(2919..>3159)
                     /locus_tag="PLA01_07915"
     CDS             complement(2919..>3159)
                     /locus_tag="PLA01_07915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD01256.1"
                     /translation="IRDNGVGIEEANLIKIWDVGYSTNNTSGLGLPFAKRIIEDNAGT
                     IDIMSQKGSGTTVTIFLPSVECIEDFRRSDTVDNK"
BASE COUNT          825 a          733 c          523 g         1078 t
ORIGIN      
        1 caccatcatc tgtgctattt tttcaagggt cgcttcttcc ttagtcattt ctccgacctt
       61 ttctccgtca cgaagaactg taatattgtc ggcaactgca attacctcat ccagcctgtg
      121 ggtaataaat attatgctta tacccttatt ggcaagattt ttcattgctt caagaagttt
      181 ctccgcttcg ctctcagcca gaacagccgt tggttcgtca aacaccagca ccttcatgtt
      241 ttccttatct atttctctgg ctatttctat aaattgcata tagcctacag gcagacctgc
      301 cacaggcagc caatcatcta cagtcattcc aagctctttc aatgctttat tggcatctct
      361 cctcatagca ggtatgtcca aagtttttaa aagtggacca aggactttac ttacagtatt
      421 atccttaagt atttcacgat tcagctttat gttctccgtt atcgtaaagc ccggcatcag
      481 catgaattcc tggtgtacca tgccgatccc aaatctaata gcctcattgg gggtttttat
      541 ttcaattact tcaccatcaa ttaatatttc acctttatag ccaccggtgt tctgaattac
      601 cggcatacca aaaataatat tcataagtgt ggatttaccc gcaccatttt caccaaccaa
      661 ggcatgtatc tctcccggat tcagctttag attgatacca ttcaaaaccc tgtttccgga
      721 atattctttt tctatatttc ttagctcaag aacgtattgt gcctcatttt ctttcggcat
      781 tgatatcctc ctcttttctg agtttgataa tcttatctat aagtccaata ttcaataaat
      841 atcatgcata aggttgccgc ttaagcgaca accttatgca gatatacgta caatataaat
      901 ctttctagaa tgttatataa tcagcaagaa ccatatagaa gttctcctga acgacgtcat
      961 tcaacttata gttgtcaaag tgaattgtgt atccgtctga tacttttgca taggcagcct
     1021 tgagagcttc cggatcattc tttgccttta attcgccatt aatatacatt atgccgtatt
     1081 cgacaagagc tgctgtagaa accattgata tcgggaatgc ataggatgag aatctccctg
     1141 aaacaccttt ttcagcaagc tttgtcttga tctgctcaac catgtattca gagtctccgc
     1201 tgtggccttc caatgagaca cccagtgctc cgggatatcc atggaatggg cttgggcagc
     1261 actgctgagc aactatagcg ccctgctcaa tacttgcctt aagtaatggc tcctgcatag
     1321 cacagtttgt gctgaagaag ttagtatctt ttccgtattc agcaactttt ctcggaatat
     1381 cttccagtat aaactgctgt gcgcctggaa ctccggcatc ccctgtcggg tctggagctg
     1441 tcgcatcaac aaactttatt ccaagtcttt cacattcctt aaccattatt tctcttctct
     1501 gaactataag aggataagac atatgtcttg ggaaggaata gtgcacaaat gtcttagcac
     1561 ccattttctt tgcctgctcg ggcatcatat aaccctgtgc cgcttcatct gctgtaactg
     1621 caacgtcaca ctttgaggcg attacgtcgg tttcgcccgg ctggtaagca agaatcagta
     1681 cgtcaggtct tacttctcta accttgtcaa atgctgcggc agcaccctgt acagactggt
     1741 tcatgataag aaccttaacg tcgggatctg atacgagacc aacagcgttg gatataagag
     1801 tttcctgctc ctttgtaaat gcatccggat atgtctgtga tacaatcatg cccgggtatt
     1861 tcttggcagc agcttccatt gatctgaatt cttcctcgtt ctgtgaaaca gttcctgtgt
     1921 aaattgcgat cttggctttt gctgcttcag ctgccggagc tgcagcttct cctgccggtt
     1981 ctgcggctgc cggttctgcc ggtttggcac atgcaacagc actgaatgcc attacaagag
     2041 ctaatactag agctaatacc tttttcaata gattcccctc cagttaatat ttatttatta
     2101 gcctgaaaat gtaacttctc aggtactaat cataaaaaaa taagcaatca tataatatgt
     2161 gaccttaaaa tatggtttca tattatataa attgcttatt ccattaattt taattattac
     2221 ctatttttat aacatatttc tctattttat aatatatttc tctgaaaaat ctttagaggg
     2281 gaaataaaaa ggctttatct accgaagctt atatttattc ttgggtcttc ccaactgtcc
     2341 ataaatagga ataatttcaa tctttttttc atcaaggaga taggatacat atctgtgtac
     2401 cgtctggtat gcaagagtga gaccgttagc tatatcttct attgttagtc ctgcctcatg
     2461 ttctttcaaa taatcacata taatactcaa ggttgctgga cttattcctt tttctatata
     2521 aacctgtttt ttttctgcaa tatatttctg ttgaacttca ttgattctta gattcatttt
     2581 tatacgggaa atcaaatccg gcgaccttat cggcttaact gcaaaatccg ttgctcctgc
     2641 agctataaat ttctctgcta tctcctgtct ctcatcaact gtcaacacta atatcgatat
     2701 tgcattatcc aactgccgga ttctttaccg tagtcaagcc atcccaatca ggcatgtgat
     2761 agtccacaat tgcaagattt ggcttgtgaa ctctgcacaa ctcataacct tccctgccgt
     2821 tgtgtgcagt tgctgctttc cagcctccaa acgcacatat ctctttgata gtataaagaa
     2881 tatcagattc gtcatcaatt attacaattt caagtttatc atttattgtc aactgtatca
     2941 ctcctcctaa aatcttcaat acattcaact gacggcagga atatagttac tgtagttccg
     3001 gaaccctttt ggctcataat atctattgtt cctgcattat cctctataat tctctttgca
     3061 aaaggcagtc ctaatcctga tgtgttattt gtcgaatatc ctacatccca tatttttatg
     3121 aggtttgctt cctctatgcc tactccgtta tctctgata
//
LOCUS       DAOOJC010000291         3144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_23825_length_3144_cov_3.863062, whole genome shotgun sequence.
ACCESSION   DAOOJC010000291 DAOOJC010000000
VERSION     DAOOJC010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23825_length_3144_cov_3.863062"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="PLA01_07920"
     CDS             complement(<1..1378)
                     /locus_tag="PLA01_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HPD01257.1"
                     /translation="MNNSRKLALAVVLVFLMVICFQNPIITSAATTVTIDPDATYQTI
                     EGWGASLCWWGNQIGHWSADNRNKLIEKIVSTTDGMGYNIFRYNIGGGENPGHNHMRV
                     YADIEGYQNADRSWNWNADATQRAVLNRLIERGEYYGSEIILEAFSNSPPYWMTKSGC
                     ASGTSDGSNNLRDDYYDDFADYLTEVVKHFRDAWGITFRTLEPMNEPTSDWWKTGNRQ
                     EGCSFTFANQQRIIKEVGEKLNAKGLTGTTVSAADETNIDIGLSGLQSYDSTTLSYMS
                     QVNVHSYYGSKRTQLRDLAKSKGLRIWQSESGPLSFNGDMADSCIMLSKRIVTDLKEL
                     QCVAWLDWQIIDGGNWGSIYVDDAAQTFTLTEKFYMHANYSRFIRPGYTIIGANNEKT
                     IAAISPDKKKLVIVATNDNKSSSENFTFNLSRFAGVNSTVEVYRTSPSLSLAKSNITA
                     SNKTVSDTL"
     gene            complement(1481..>3144)
                     /locus_tag="PLA01_07925"
     CDS             complement(1481..>3144)
                     /locus_tag="PLA01_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPD01258.1"
                     /translation="VRNADFDVRIDENVTPETDAQWELVPGLANSKDGYVSIQSVNHP
                     GYYLRHWDYDFRLEKNDGTAIFAEDATFKMVPRLADSSYTSFQSYNFPDRYIRHYDYL
                     LRLDEIVTSLDREDATFKVIDGSSVDPDKAEDSVIVTNPIVRRRADPWVYRHTDGYYY
                     MTASVPEYDRIELRRAKTLQGLSTATPKTIWKRHSSGVMGGHIWAPEIHFIDGKWYIY
                     FSAGTSSNYFDIRLYVLECSDLNPVSGTWVERGKLKTDWESFTLDATTFEHKGVRYLV
                     WAQKDPNIASNSNIYIAKMNGPLAITGSQVMIASPQYSWEKVGYAVNEGPAVIKKNGK
                     IFMTYSASATDSNYCMGLLTASDNSDLLDPKSWHKSPNPVFLSNTSTGQYGPGHNSFT
                     TSPDGKVDIMVYHARNYRDITGDPLYDPNRHTRAQIVNWKADGTPDFGILVADGENAI
                     YIPTQTPTQSPTPTPTQSPTPTPIPTITATPNNLIYGDINGDSFVDSTDLTLMKRHLL
                     GKSVPMAPNWQQVCDFNLDGSIDSTDLTILKRYLLGKIKTLPMVY"
BASE COUNT          815 a          736 c          620 g          973 t
ORIGIN      
        1 gcaaggtgtc ggaaacggtt ttattcgatg ctgttatatt gcttttggca aggctaaggc
       61 tcggagatgt tctgtaaacc tccacggtgc tgtttacacc tgcaaacctt gacaggttga
      121 atgtaaagtt ttcgctggat gacttattgt cattggttgc cacaataaca agcttctttt
      181 tatccgggct tattgccgca attgtttttt cattattggc accaataatt gtataaccgg
      241 gccttataaa cctgctgtaa tttgcgtgca tatagaattt ttccgtaagg gtaaaggttt
      301 gggccgcatc atctacataa atacttcccc agttgcctcc gtctattatc tgccaatcca
      361 gccatgctac acactgcaat tctttcaagt cggtaacaat acgttttgac agcattatac
      421 acgaatcggc catatcgccg ttaaaactca gcggaccgga ttccgactgc catattctaa
      481 gtcctttgga ttttgcaagg tctctgagct gagttctttt tgagccgtaa taagaatgca
      541 cattaacctg ggacatatag gacagagtag ttgagtcata agactgaagc cccgaaagac
      601 cgatgtcaat attggtttca tcggcagcac tgacggttgt cccggtcaat cctttggcgt
      661 ttaatttctc tcccacctct ttaataattc tctgttgatt tgcaaaggta aaggagcaac
      721 cttcctgtct gttaccggtc ttccaccagt ctgaagtggg ttcattcatg ggctctagag
      781 tcctgaaggt aataccccat gcatccctga aatgctttac cacctcagta aggtaatcgg
      841 caaaatcgtc ataataatcg tctcttaaat tattggaacc atcggaggtt ccggaagcac
      901 agccgctttt tgtcatccag tacggaggag agttggagaa cgcttcgagt attatttccg
      961 atccataata ctcccctctt tctatcagcc ggttaagcac agccctttga gtggcatccg
     1021 cattccagtt ccacgaacgg tctgcattct gatatccttc aatatccgca taaaccctca
     1081 tatgattatg tccgggattt tctccgccgc cgatgttgta cctgaatata ttatatccca
     1141 taccgtcggt cgtgctgaca attttttcaa tcaatttgtt cctgttatcg gccgaccagt
     1201 ggccaatctg gtttccccac cagcataaac ttgctcccca gccttcaatg gtttgatagg
     1261 ttgcatcagg gtcaatggta acagttgttg ctgcactggt tattatagga ttctgaaagc
     1321 atattaccat taggaaaacc aatacaaccg ccaatgcaag ctttcttgaa ttattcataa
     1381 aatcccccct ttaaaatcgt ttatataaag gcgataaatg tgccccactt ccataagcgg
     1441 cagctacaga tttgcccgaa gcagtggagt gtacatttat ttaatacacc ataggcagtg
     1501 ttttaatttt gcccaaaaga tacctcttaa gtattgttaa atccgttgag tctatacttc
     1561 catccaaatt aaaatcgcat acctgctgcc agtttggtgc cattggaaca ctctttccaa
     1621 gcaagtgcct tttcataagt gtcaaatccg ttgaatcgac aaaactgtct ccatttatat
     1681 cgccataaat taagttgtta ggcgtggcag taattgtagg tattggtgtc ggtgtcggtg
     1741 actgtgtcgg tgttggcgtt ggtgattgtg ttggtgtttg ggtcggaata tatatagcat
     1801 tttctccgtc ggcaaccagt atcccgaaat ccggggtacc gtcagctttc caattcacta
     1861 tttgtgcccg ggtgtgtctg tttgggtcat acaaaggatc acctgttata tcccggtagt
     1921 tcctcgcatg atataccata atatccactt ttccatccgg cgaagtggta aaggaattgt
     1981 gtcccggtcc gtactgccct gtggatgtat tgcttaaaaa tacaggattc ggcgatttgt
     2041 gccatgattt cggatccaat aaatcactgt tatccgatgc agtcagcaat cccatgcagt
     2101 agtttgaatc tgtggcactt gctgaataag tcataaatat ttttccgttc ttctttatta
     2161 ctgcagggcc ttcattaacc gcataaccca ccttttccca ggaatactga ggtgatgcaa
     2221 tcataacctg acttccggtt atggcaagag gcccattcat ttttgcaata taaatattgc
     2281 tgttacttgc tatattggga tctttctgag cccatacaag gtacctgacc cctttatgct
     2341 caaaggtagt tgcatcaagg gtaaaagact cccaatcggt ctttaattta cctctttcca
     2401 cccaagttcc gctaacagga tttaaatccg agcattcaag cacatacaag cgtatatcaa
     2461 agtaattact ggatgttcct gctgaaaaat aaatatacca ttttccatca atgaaatgaa
     2521 tctcaggagc ccagatatgc cctcccataa ctccgctgga gtgccttttc catattgtct
     2581 taggtgtagc tgttgacagc ccctgcaaag ttttcgccct tctgagctct attctgtcat
     2641 actccggtac ggatgccgtc atataataat aaccgtcggt atgcctgtaa acccagggat
     2701 cagcacgccg tctgactatt ggatttgtca caataaccga atcttcagcc ttgtcaggat
     2761 ccacagatga accgtcaatc actttaaatg ttgcatcctc tctgtcaaga ctcgttacaa
     2821 tttcatccaa ccttaacagg taatcataat gccttatata tctgtcagga aaattatacg
     2881 actgaaaaga agtgtatgaa gaatccgcaa gcctcggaac cattttaaag gttgcatctt
     2941 cagcaaaaat tgcggtcccg tcgttctttt ccaatctaaa atcataatcc cagtgtctta
     3001 agtagtatcc cggatgattg acagattgaa tagaaacata cccgtctttg ctattggcca
     3061 ggcccggaac cagttcccat tgggcatctg tttccggggt aacattttcg tctatcctga
     3121 catcaaagtc tgcattcctt acat
//
LOCUS       DAOOJC010000292         3142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_23845_length_3142_cov_2.650146, whole genome shotgun sequence.
ACCESSION   DAOOJC010000292 DAOOJC010000000
VERSION     DAOOJC010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23845_length_3142_cov_2.650146"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLA01_07930"
     CDS             <1..342
                     /locus_tag="PLA01_07930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPD01259.1"
                     /translation="QNKIAAMLKPGMIPAEIYRDVMESLDESFKQNFMGFKERRVKFL
                     GHGIGLHVDEEPVIAEGFNEPLLEGMLLALEPKKGIEKVGMVGIENTFKVTGQGGVCI
                     TGDNPGLIPVF"
     gene            402..1157
                     /locus_tag="PLA01_07935"
     CDS             402..1157
                     /locus_tag="PLA01_07935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HPD01260.1"
                     /translation="MSHIITENVTDGKNSVQYILSRSKRKTVGIKLNEKGEVSVSAPL
                     GLPDKQLREIIIKKIPWIVKKQEELKRKYNESGEQKKFIDSEAFLYLGKEYTLKIQRG
                     SASENVAFCDNDIVVSITGDTAENENIDEEERAKTIKKILRGFYINRFLEVIKDRIDN
                     YAPKIGVYPKKVTIREQKTRWGSCSSKGNISINWKLIMAPSKVIDYVIVHELCHMREM
                     NHSKNFWNIVSEVCPDFKESQKWLKDNGHRMTI"
     gene            1189..1314
                     /locus_tag="PLA01_07940"
     CDS             1189..1314
                     /locus_tag="PLA01_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01261.1"
                     /translation="MTAGKWTFWQYCNRGHVKGIGTYVDINCFSGTTEELLELAK"
     gene            1339..2088
                     /locus_tag="PLA01_07945"
     CDS             1339..2088
                     /locus_tag="PLA01_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD01262.1"
                     /translation="MKLSQMEQYIRNIELNRQKVDSFNRYPFNLPSIRTLTKLEFHPC
                     VTYIVGENGSGKSTVLEAIAVATGFNPEGGTRNNNFSTRASHSDLSDYLTIVRGVRRP
                     LNGFFLRAESFYNLATDIEELDKDERGPRIIDTYGGVPLHEQSHGEAFFAVFMNKFRK
                     RGIYILDEPESALSPSRQMAMISRMHQLVEEGSQFIIATHSPIIMSYPDSIIYQIKNG
                     FERVKYNETDNYQIMKAFVNNTEKMLGILMG"
     gene            complement(2135..>3142)
                     /locus_tag="PLA01_07950"
     CDS             complement(2135..>3142)
                     /locus_tag="PLA01_07950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD01263.1"
                     /translation="PKPLTFDTVEEDIMSFKPDIINLESSEGLFVGFWERAGLNAAKK
                     LGIPITSLYHTNWIAYFSDYKKQIPLLRIPGIERVFTKLITWINNSYDLTLVPSKNAE
                     QFLKQCGIKNTHYGRFLGIDVSKFSPYQKQEIKELDVLKDKIKVLYIGRLTPDKQIDT
                     LLRVFDETRKVTDKCGFVIVGCGSDEERVKSWASQYSDTVFIGRIPNEETPKYYSACD
                     IFITASNKENHPLTVWEAMASGLPVVGPDERGVGELVYNNETGILVNTGAVDEFVSAV
                     LKLSTDETLRHKMSRNALNMVKDCTWENAAQNQLNVWKDLIQQKSKDIQDSVSMKRKL
                     G"
BASE COUNT          950 a          566 c          745 g          881 t
ORIGIN      
        1 caaaacaaga tagcggcaat gctaaaaccc ggcatgatac ctgctgagat ttatagagac
       61 gtaatggaaa gtcttgatga gagtttcaaa cagaacttta tggggttcaa ggaacgcaga
      121 gtcaagtttt tgggacatgg aataggcctg catgtcgatg aggagccggt tattgccgag
      181 ggctttaatg agccgctttt ggagggaatg ttgctggctt tggagcctaa aaagggtatt
      241 gagaaagtag gaatggtggg aattgaaaat actttcaagg ttaccgggca gggcggagtg
      301 tgcataaccg gggataaccc cggattgata cctgtatttt aggtataagg catcaggggg
      361 cagaaatagc cataaataga ggaaaaagca tagatatggg agtgagtcat atcataacag
      421 agaatgtaac ggatggtaag aatagtgttc aatacatcct ttccagaagc aaacggaaga
      481 ctgtgggcat aaaattgaat gaaaagggag aggtgagtgt atcggctcct ttgggattgc
      541 cggacaaaca gttaagggag attattatta agaagattcc gtggatagta aaaaaacagg
      601 aagagctaaa acgtaaatac aacgagtcag gtgaacaaaa aaagtttata gatagcgaag
      661 cctttttgta tcttggaaag gagtacacct tgaaaataca gagaggttca gcttctgaaa
      721 atgttgcatt ttgtgataat gacattgttg tttcaataac cggggataca gccgaaaatg
      781 agaatataga tgaggaagaa cgggctaaaa ccataaaaaa aattctaaga ggcttttata
      841 taaatcggtt cctcgaagtc attaaggata ggatagataa ttacgctcct aaaataggtg
      901 tatatcctaa aaaagttaca atccgggagc aaaagacccg gtggggaagc tgcagcagca
      961 agggaaatat cagcattaac tggaagctca taatggcacc ttctaaggtc attgactatg
     1021 ttattgtaca tgagttgtgt catatgagag aaatgaatca ctccaagaat ttttggaata
     1081 tagtaagtga agtatgtcct gacttcaaag agagtcaaaa gtggctcaag gataatgggc
     1141 accgcatgac aatataaatt taggtttata tcaagcctcc gtctttgagt gacggccgga
     1201 aaatggactt tctggcagta ctgcaacaga ggacatgtta agggaatagg cacgtatgtg
     1261 gatattaact gtttttccgg gacaacggag gaacttcttg aattggcgaa ataatgctaa
     1321 acacaagggg atgagtagtt gaagctttca cagatggaac agtatataag gaacattgaa
     1381 ttgaacagac aaaaggtgga ttcttttaac cggtatcctt ttaatttgcc gtcgatacgt
     1441 accctgacaa aattggagtt ccacccgtgt gttacatata ttgtcggaga gaacggcagc
     1501 ggaaagtcaa cggttctcga ggcaattgcc gttgcgaccg gttttaatcc tgagggcggc
     1561 acaagaaata ataatttttc cacccgggct tcccattccg atttgagcga ttatctgaca
     1621 attgtcagag gagtcaggcg acctttgaat ggcttttttc ttcgggcaga gagcttttat
     1681 aatctggcaa cggatattga agagcttgat aaagatgaaa ggggaccgag aataattgat
     1741 acctacggcg gtgttccgct gcatgaacag tcccacggag aagcgttttt tgcggtattt
     1801 atgaataaat tccgcaaaag gggcatttat attctggatg aaccggaatc ggcattgtct
     1861 ccctcaaggc agatggcaat gatttcaaga atgcatcaac tggttgaaga aggctctcag
     1921 tttattattg cgacccactc tccaataata atgtcatacc ctgactcaat catttaccag
     1981 ataaaaaacg gtttcgagag ggttaaatac aatgaaaccg ataactatca gataatgaaa
     2041 gcttttgtta acaacacaga aaaaatgctt ggtatactta tgggataaga tgaaactgtc
     2101 ttcatgcact gccaaaatcg aggcacaagt tatgctagcc cagctttctt ttcattgaaa
     2161 cggagtcctg tatgtctttg cttttttgct ggattaggtc tttccacaca ttcagctggt
     2221 tttgagcagc gttttcccag gtacagtctt taaccatatt tagggcattt cgactcattt
     2281 tgtgccttag tgtctcatcg gttgaaagtt tcaacacagc agacacaaac tcatctacgg
     2341 caccggtatt tacaagaatc ccggtttcat tgttgtaaac gagttctccc acaccccgct
     2401 catccgggcc aaccacagga agtccgctgg ccatggcttc ccatacggtc aaagggtggt
     2461 tttccttatt ggatgctgta ataaatatat cacatgcaga gtagtatttg ggcgtttctt
     2521 cgtttgggat tcttccgatg aatacggtat cggaatactg agaagcccat gattttaccc
     2581 tttcttcatc gcttccgcag ccaactatta caaaaccgca tttgtcggta accttgcggg
     2641 tttcatcgaa aactctaagg agggtgtcaa tttgcttgtc aggggttaac ctcccgatat
     2701 aaagtacctt tattttatct ttgagaacat caagttcttt tatttcctgt ttttgatacg
     2761 ggctgaactt tgatacgtct attccgagaa aacggccgta atgggtgttt tttatcccgc
     2821 actgttttaa aaactgttcg gcgtttttgg aaggaacgag agtcagatcg tagctgttgt
     2881 ttatccaggt tatcagcttt gtgaatactc tttcgatgcc gggtattcta agtaaaggaa
     2941 tctgtttttt gtagtccgaa aagtatgcta tccagtttgt gtgatagagg cttgtaatgg
     3001 gaatgcccag tttcttggcg gcatttaacc ctgcacgttc ccaaaaaccc acaaaaagac
     3061 cttcggagga ttccaaatta attatatccg gcttaaagga cattatgtct tcctctacgg
     3121 tatcaaaggt caaaggtttt gg
//
LOCUS       DAOOJC010000293         3122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_24027_length_3122_cov_3.538637, whole genome shotgun sequence.
ACCESSION   DAOOJC010000293 DAOOJC010000000
VERSION     DAOOJC010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24027_length_3122_cov_3.538637"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /gene="spoIIIAF"
                     /locus_tag="PLA01_07955"
     CDS             complement(<1..591)
                     /gene="spoIIIAF"
                     /locus_tag="PLA01_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HPD01264.1"
                     /translation="MIEFIKNWVVNTITVVMLVILIEMLIPSGKMKKMVNLVSGFILV
                     IALINPVLKVLGKEIMLREMQMADSNYIDKKEIMASSGVLEENQIKQITNLYRDKIIE
                     QLESITKEVKGIEDARADVIINEDYTSDRFGEVKKVYLYIKTQKRNDGVKPVLSVKKI
                     EIGNGNSKDGSSMDEDSKDEMLSEKQLDKKSKQELEE"
     gene            complement(685..1896)
                     /gene="spoIIIAE"
                     /locus_tag="PLA01_07960"
     CDS             complement(685..1896)
                     /gene="spoIIIAE"
                     /locus_tag="PLA01_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HPD01265.1"
                     /translation="MINKRGLRKCIVPVTLILAMACMGVSQFSQKAYAEEESVEYGII
                     DRQTQTDEVKNIEEQLEKYKNERLDDILPGYDPENMINDMAKGNYDFDVPGVANRLLG
                     YLFEELYLNMDILIKLIVLVVLCAVLNNLQTSFLNKGTGELAFYVCYIVLVSILLVSF
                     NSIVKSGIELIDGMVNFMYASIPVLITLLVSSGNFASGGALQPILVMVVQVSATIIKN
                     VLIPLIVFSTIISIVDNVSDKIQITRFSAFLKHITQWSLGIILTVFVAIVSLQGSLGA
                     VVDGVTSKTAKFAINTFIPVAGKYLADAADTVIGCALLIKNAVGVAILIGIIGICLIP
                     IVEMLAVILIYRLTCVLLEPLADGRIIKCVGEVASSMTYILGTVASVAFMFLITVTVL
                     ILASNISVMVR"
     gene            complement(1928..2314)
                     /gene="spoIIIAD"
                     /locus_tag="PLA01_07965"
     CDS             complement(1928..2314)
                     /gene="spoIIIAD"
                     /locus_tag="PLA01_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HPD01266.1"
                     /translation="MEIFQIVGLGVVATIIAIVLKAHRPEIAIQISLLTGIIILTVLL
                     GKIAAVVDLLNSYAQKVNIDLIYFSTLLKIIGIAYIAEFGAEVCRDAGEGAIASKVEL
                     AGKVIIILLAVPIITSLLDLIISVMP"
     gene            complement(2339..2533)
                     /gene="spoIIIAC"
                     /locus_tag="PLA01_07970"
     CDS             complement(2339..2533)
                     /gene="spoIIIAC"
                     /locus_tag="PLA01_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HPD01267.1"
                     /translation="MNIDLIFRIAAIGILVSVLNALLNKSGREEQAMMTTLAGLVVVL
                     LMIVKEIAHLFDTVKALFQL"
     gene            complement(2638..>3122)
                     /gene="spoIIIAB"
                     /locus_tag="PLA01_07975"
     CDS             complement(2638..>3122)
                     /gene="spoIIIAB"
                     /locus_tag="PLA01_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="stage III sporulation protein SpoIIIAB"
                     /protein_id="HPD01268.1"
                     /translation="SSSLLGYIYSRKCSERPKDLRTLQGLLQMFENEISFLSNVLTEA
                     FMKICRCSDSSVSAFFGHTVEILNSDTGINAQDAWTKAVKENINKTALNSEDEEILIS
                     FGKLLGSSDTEGQIKNIRLTLNQLKMQEEKAEELRKKNESMYRNLGVLGGLAIIIILF
                     "
BASE COUNT          887 a          758 c          474 g         1003 t
ORIGIN      
        1 ctcttccagc tcctgtttgg attttttatc cagctgtttt tcggaaagca tctcatcttt
       61 tgaatcttca tccatcgaac tgccatcttt tgaatttcca tttccgattt caattttttt
      121 aacacttaag acaggcttta ccccgtcatt tctcttctgg gttttaatat aaaggtaaac
      181 ctttttcacc tctccaaacc tgtcggatgt gtagtcttca ttgataatta catcagcccg
      241 tgcatcttcg atacccttaa cctcttttgt tattgactcc aattgttcaa ttatcttatc
      301 cctgtataaa ttggttattt gctttatttg attttcttcc aaaaccccgc tacttgccat
      361 tatttccttt ttgtctatgt agttgctgtc agccatttgc atttccctca gcataatttc
      421 tttgccgaga actttaagaa cagggtttat caaagcaatt accagtatga aaccggaaac
      481 caggtttacc atctttttca ttttgcctga aggtatcagc atttcaataa gtatgacaag
      541 cattacaaca gttatggtat ttacaaccca attttttata aactctatca ttttctcctc
      601 cgtacttctt aaaatttatt gcctgactca agcaaaataa ttttacaagg tttagccgca
      661 actccccgta cttttttgaa ttaattatct gaccatgaca gaaatgttac ttgccagtat
      721 cagcaccgta acggttatca aaaacatgaa cgccaccgat gcaaccgttc ccaatatata
      781 tgtcattgag cttgcaactt cacccacgca ctttattata cggccgtcag caaggggttc
      841 aagaaggacg caggtcaacc tgtatatgag tataaccgct aacatttcca ctatcggaat
      901 aaggcaaata ccgatgattc caatcaaaat tgcaaccccc acagcatttt taatgagtaa
      961 agcacagcct atgaccgtat cggccgcatc cgccaaatat tttccggcca caggaataaa
     1021 ggtgtttatg gcaaactttg ctgtcttgct tgttacaccg tcaaccactg ccccaaggga
     1081 cccctgcagg gatactatcg caacaaacac tgtaagaatt attcctaagg accattgggt
     1141 aatatgtttt aaaaaagccg aaaaccgcgt aatttgtatt ttatccgata cattatccac
     1201 gatggatatt atggttgaaa agacaataag cggtataagc acgtttttta taattgtcgc
     1261 actcacctgc accaccatga caagtatagg ctgcaatgca cctcccgaag caaaattccc
     1321 cgatgaaacc agcagtgtta tcaaaactgg tatcgaggca tacatgaaat ttaccattcc
     1381 gtctattaac tctatccccg attttactat agaattaaag cttaccagga gaatggacac
     1441 cagtactatg tagcaaacat aaaaggccag ttccccggta cccttgttta aaaaagatgt
     1501 ctggaggttg tttaaaaccg cacataatac caccagcaca ataagcttta tcaatatatc
     1561 catatttaag tatagctctt caaaaagata tcccagaagt ctgtttgcca cccccggaac
     1621 atcgaaatca taatttccct ttgccatatc gttaatcata ttctcaggat cataaccggg
     1681 cagaatatca tcaagtcttt cattcttgta tttttcaagc tgttcctcga tgtttttaac
     1741 ctcatcggtt tgagtctgcc ggtcaatgat gccgtattcc acgctttctt cctctgcata
     1801 ggctttttgc gaaaattgtg acactcccat gcaagccatg gctaatatca atgttacagg
     1861 tacaatgcat tttcttaatc ctcttttgtt tatcatacac cccaacccca ttccttcacc
     1921 tctagcctca aggcataacg cttattataa ggtctaaaag cgaggttatt ataggtactg
     1981 ccaataaaat aattatcact tttcctgcaa gctcaacctt cgatgcaatt gcaccttctc
     2041 cggcatccct gcacacttct gcaccaaatt ccgcgatata ggctattccg attattttaa
     2101 gcagagtgct gaaatagatc aaatcaatat ttactttttg agcatagctg tttaaaagat
     2161 ccacgactgc tgcaattttc cccagtaaaa ccgtcagtat tatgattcct gtaagcaaac
     2221 tgatctgtat ggcaatttca ggcctgtgtg ccttaaggac aatcgcaatt attgtcgcaa
     2281 ccacacccaa ccccactatt tgaaatattt ccaccttaat caaccgcctc ttttgtattt
     2341 atagctgaaa cagggctttt accgtatcaa acaggtgtgc tatttccttc actatcatca
     2401 aaagaaccac caccagtcct gccaaagtag tcatcatggc ctgttcttct cttcccgatt
     2461 tattcagcaa tgcatttagt acagatacca gaatacctat tgctgcaatc ctgaaaatca
     2521 aatctatatt cataatctca tctttctccc tcatattctt ctactgtaat cattacacaa
     2581 attggtatcg ggcaaatccc tttatatagt acaagtatat tcacttccac cacaagatta
     2641 gaacaatata ataattattg ccaaacctcc aagcacaccc aaatttctgt acatggattc
     2701 attttttttc ctcaattcct ccgccttttc ttcctgcatt ttaagctggt ttaatgtaag
     2761 tctgatgttt tttatttgtc cttctgtatc ggagcttccc agaagcttgc cgaaggaaat
     2821 cagaatctct tcgtcctcac tgttcaaggc cgttttattt atattctctt taactgcctt
     2881 tgtccaggca tcttgtgcgt ttattcccgt atcgctgttt aaaatctcaa cagtgtgccc
     2941 gaaaaaagcg gatacactgc tgtcgctaca ccggcatatc ttcataaagg cctcagtcag
     3001 cacattggat aagaagctta tctcgttttc aaacatctgc aaaagtccct gcaaggttct
     3061 caaatccttc ggacgttccg aacacttccg ggaatatatg tatcctaaaa gactgctgga
     3121 ga
//
LOCUS       DAOOJC010000294         3115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_24120_length_3115_cov_3.876797, whole genome shotgun sequence.
ACCESSION   DAOOJC010000294 DAOOJC010000000
VERSION     DAOOJC010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24120_length_3115_cov_3.876797"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PLA01_07980"
     CDS             complement(<1..768)
                     /locus_tag="PLA01_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPD01269.1"
                     /translation="MIDILSREFIYLWYYFTVLFEQIFKYWVLGILLGSVISVFCKEK
                     IHSVFIGMKNSRWGLLGIIPASILGIASPLCMYGTIPIAASFSKKGMRDDWLAAFMMS
                     SILLNPQLLIYSMALGKTAVLIRLFSCLICGITAGLFIHVFYRKKSFFNFKSFGDMTN
                     RDTDPNVLVRLLKNIGRNIKATGGYFLIGIILSVLFQRYVPADSFARLFGSDQRFGIL
                     MASTIGVPLYVCGGGTIPLLVEWLDAGMSMGSAAAFMI"
     gene            complement(761..1621)
                     /locus_tag="PLA01_07985"
     CDS             complement(761..1621)
                     /locus_tag="PLA01_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPD01270.1"
                     /translation="MTKDISPEGLIRIYEALGRQATGKVGIKVHMGEPGGHNYLNPDL
                     IKDFVLSVNGTFVDCNTAYGGLRATTAMHMQAAKDHGFTAYTSVDIMDAEAEISLPIE
                     NGKHLKEALVGSHFKDYDFFVVLSHFKGHAMGGFGGAIKNMSIGFSSQKGKNLIHNAG
                     LRNTNSWLYGGYIQDHFLESMAEAAKAVADYAGENILYISVMNNLSVDCDCDSNPADP
                     EMEDIGILASLDPVALDKACVDQVYAADEEESARLRRRIESLNGTHTLDYAEEIGLGT
                     QKYKLEVIDD"
     gene            complement(1911..2462)
                     /locus_tag="PLA01_07990"
     CDS             complement(1911..2462)
                     /locus_tag="PLA01_07990"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPD01271.1"
                     /translation="MKDNTSYYVILGILSFGECSGYEIKKKIENEIGYFYKVSNGQIY
                     PVLKKLLHQNNASCRVEKGDRKPYRKVYSITDQGYTVLKEWLDKTDYPHNEFLLRLYF
                     GSIRPISKNIEMINDFKAVKERHLETYNNISEYFNLKTIDKLQDYYSYLTLRHGQLIA
                     KAYIDWCNEVVDILQELKKENER"
BASE COUNT          905 a          733 c          537 g          940 t
ORIGIN      
        1 tatcataaaa gctgccgcag atcccatact catgccggca tccagccatt ccaccagcaa
       61 gggaatcgtt cctccgccac atacatatag cgggacaccg atagtagaag ccatcaaaat
      121 tccaaatctt tgatcacttc caaacaaacg tgcaaatgag tctgctggaa catagcgctg
      181 aaacagtact gacaaaatga tcccaatcaa aaagtaacca ccggtggcct taatattcct
      241 gccgatattt ttgagcaacc gtacaaggac attgggatcg gtatccctgt ttgtcatatc
      301 tccaaagctt ttaaaattga agaaactctt ctttctataa aatacatgga taaataaacc
      361 ggctgttata ccgcagatca gacatgaaaa taaacgaatg agcactgcag ttttgcccaa
      421 ggccatacta tagatcaata actgtggatt gagtaatatg gagctcatca taaaagctgc
      481 cagccagtcg tcccgcatgc cttttttgga aaatgaagcc gcaataggta tagtcccata
      541 catgcacaac ggcgaggcta tacctaaaat acttgcaggt atgatgccaa gcagtcccca
      601 cctgctgttc ttcatcccaa tgaagactga atggatcttc tccttgcaga atactgatat
      661 gacagagccc aacaggatac caagcaccca gtatttaaaa atctgttcga acaatacggt
      721 aaagtaatac cataaatata taaactcccg gctgagtata tcaatcatct attacctcca
      781 atttatactt ctgagtccca agaccgattt cctcagcgta atcgagcgta tgcgtcccat
      841 tcaatgattc gatacgtctt cttaacctcg cactctcctc ttcatctgcc gcatagacct
      901 gatccacaca ggccttgtca agtgcgacag gatcaagcga ggccagtatc ccgatatcct
      961 ccatttccgg gtcggccgga tttgaatcac agtcgcagtc tacggaaaga ttgttcataa
     1021 cgctgatata caagatgttt tccccggcat aatcagcgac tgcttttgcc gcctcagcca
     1081 tggattcaag gaaatggtcc tgtatgtatc ccccatacag ccaggagttg gtgttgcgaa
     1141 gtccggcgtt atggatcagg ttcttgccct tttgagaaga aaaaccgatg gacatatttt
     1201 tgatcgctcc tccgaagcca cccatggcat gacccttaaa gtgtgaaaga acaacaaaga
     1261 aatcatagtc tttaaagtgg gaccctacca gggcttcctt cagatgcttt ccgttttcta
     1321 taggcaagct gatttcagct tccgcatcca taatatccac cgatgtatac gcggtaaatc
     1381 catgatcctt tgcagcctgc atatgcatcg cagttgttgc acgaagtccg ccgtaggcag
     1441 tgttgcaatc cacaaaggta ccgtttaccg aaagtacaaa atcctttatc agatcaggat
     1501 tcagatagtt atgtcctccc ggctcaccca tatgtacctt tatcccgact ttgcccgtag
     1561 cttgccggcc taaggcctca tatatccgta tcaagccctc cggactgata tcctttgtca
     1621 tgtaaacaat cggcacggtc tccgaatcgg gttctgcagt tggttctgta ttctcagccg
     1681 tctctttgcc atcagtggct ctttcgtcta tgatattttg atttggatta tcaacttcag
     1741 aattttttgg tgtacatccg gatacgattg aaaaagccag cactatcaaa agtgcaatta
     1801 atctacttcg tttcactgta cattccccca gactttttga catatgtcat tatgacatat
     1861 tataactgaa ttgaaccttc ctgtcaagag gactagtttg atccggacaa ctacctttca
     1921 ttttctttct tcagttcctg caatatatcc actacttcgt tgcaccaatc gatatatgct
     1981 tttgctatca actggccatg tcgcagagtc aaatagctgt aatagtcctg cagcttatca
     2041 atggttttga ggttgaaata ctccgaaatg ttgttatagg tctccagatg cctttccttt
     2101 actgccttaa agtcgttaat catttcgatg ttcttactta tcggacgtat gcttccaaag
     2161 tatagtctca aaaggaactc attatgagga taatccgtct tgtcaagcca ttccttcaat
     2221 actgtatatc cctggtctgt tatggaataa acctttcgat agggttttcg gtcacctttt
     2281 tcaactctac agcttgcgtt attctgatgt agaagctttt tcaatacagg ataaatctga
     2341 ccattgctga ccttgtagaa ataccctatc tcattctcaa ttttcttttt aatctcataa
     2401 ccagaacact cgccaaagct gagtatccct aaaataacgt aataactggt gttatctttc
     2461 ataaaatgct cctttgagtt atttacagta acttatagcg tgtatactat aagtttttca
     2521 caatcggcct ctctgataat cgtttatatc cttccaatat tttctcttat aatgatatta
     2581 acatatacat aaatggtcat gcaacttagc attataatct ctagttctac ccatacctag
     2641 ccttctatcc ggaacggatg gaaaaaacca tttataaact gaagaacgaa aaagtatcat
     2701 tgaacggact gtatcagaga gcatagtttg tagaatatgt gaacacaggt aaaatttgca
     2761 gtattagcgg taaatttaaa gaggacagcc gctctggtat ccccttaccc aaatagaact
     2821 caccatatag tataataata acctgttata ataaaaacct cccacatctt ccttcaattt
     2881 ccgcacaatt aatccccaca ctctcatcta tctgtttgta tttccaagac agcagttaac
     2941 tattacctca agttgctgct cattgtaggt ataaattaaa agaaatttat tttagacaaa
     3001 ttttcagcca ctgttttatt cgcttgtaaa agtcttcctt tttctaaatc ttcgattgag
     3061 tgactatctg aaccaataga aacttcaatt tgatttagat tcaggttatg aaata
//
LOCUS       DAOOJC010000295         3098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_24296_length_3098_cov_3.256655, whole genome shotgun sequence.
ACCESSION   DAOOJC010000295 DAOOJC010000000
VERSION     DAOOJC010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24296_length_3098_cov_3.256655"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLA01_07995"
     CDS             complement(<1..458)
                     /locus_tag="PLA01_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPD01272.1"
                     /translation="MKWNDKRYHTLDYELKQTFGRKVIKLSLDGGFTCPNRDGSIATR
                     GCIFCGEKGSGDFAGTRDVSIAEQIEQQKKLLSSKWPDGKYIAYFQSYTNTYAPLDRL
                     RTRYEDALSCKDVVGLAVATRPDCLPDDVLSLLEEIAGKTFMWIELGLQT"
     gene            655..>3098
                     /gene="adhE"
                     /locus_tag="PLA01_08000"
                     /gene_synonym="adhC"
     CDS             655..>3098
                     /gene="adhE"
                     /locus_tag="PLA01_08000"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HPD01273.1"
                     /translation="MKETVKEADKKIDINKEINRLVEKASKALDSFIEFDQSQVDNIV
                     KEMAMAGLDEHMRLAKMAVEETGRGVYEDKTIKNIFATEYVYHSIKYDKTVGIINENE
                     FEDYVEVAEPVGVVAAVTPVTNPTSTTMFKSIISMKTRNPIIFAFHPSAQKCSAEAAR
                     ILRDAAIEAGAPEGCIQWIENPSIEATQALMHHPGVAMVLATGGSSMVKAAYSTGKPA
                     LGVGPGNVPCYIEKSVDVKRAATDLIMSKTFDNGMICASEQAVIVDKDISKEFEEFMK
                     ENKCYFLNEEEIKKLEKIAIDEAKCAMSPAVVGQSAYKIAKSAGFEVPEDTKILITKL
                     NGVGPEYPLSREKLSPILAYYNSDGWEHGIRIAEEMVEFGGLGHSAVIHSKNQRVIDE
                     FSKRIKAGRIIINSPSTHGAIGDIYNTNMPSLTLGCGSFGRNSTTANVSAVNLINRKR
                     VARRRVNMQWFKIPEKIYFQFGATQYLEKMPGISKAFVVTDPYMVKLGYVDRVLYFLR
                     KRQQYVHCEIFTEVEPDPSLDTVMRGYDLMNKFKPDVIIALGGGSAIDAAKGMWLYYE
                     EPETDFASLRLKFMDIRKRVFKFPRLGKKAKFVAIPTTSGTGSEVTSFAVITDKVNNT
                     KYPLADYELTPDVAIIDPEFVMTVPPSVTADTGMDVLTHAIEAYVSVLASDFTDALAI
                     KAIQLVFEYLPQAYKNNSNELAREKMHNASCIAGMAFTNAFLGINHSLAHKLGGEFHI
                     PHGRANAILLPHVIEYNASKPSKFVSFPKYETFIADKRYAEIAKALGLPASTVEEGIR
                     SLIKAVKDLMRELNIP"
BASE COUNT          970 a          648 c          679 g          801 t
ORIGIN      
        1 atggtctgca gtcccagttc tatccacata aaagtctttc cggcaatctc ttcaagcagc
       61 gataaaacat catccggcaa gcaatcgggt cttgtagcaa ctgccaatcc aactacatcc
      121 ttgcaggaca atgcatcttc ataccgggtt ctaagcctgt ctaaaggagc ataagtattg
      181 gtatagctct ggaaatatgc aatatacttg ccgtcaggcc attttgaaga taagagcttt
      241 ttctgctgct caatctgctc agcaatgctc acatcccttg ttcccgcaaa atcgcccgaa
      301 cccttttcgc cgcaaaaaat acaacctctt gtggctatgc tcccatccct gttgggacaa
      361 gtaaaaccac catccaagga aagctttata accttccgtc caaaggtctg cttgagttca
      421 taatctaaag tgtgataccg cttatcattc catttcattc tattatctcc tcatgcatac
      481 gcaatggtca taccttttca aaacattaat ttattttctt gtctcaactt attataacaa
      541 aaaacacagg tatcaaccac acgcgtgggg tgcacattgt ttgtatgtaa aaaaaaccat
      601 cacgaattca tttttatata taggacaaac ttattatgtt aggaggtttt atacatgaaa
      661 gaaacagtta aggaagcgga taaaaaaatt gatattaaca aggaaattaa cagactggta
      721 gaaaaggcaa gtaaagcttt ggacagtttt atagagtttg accagtccca ggttgacaat
      781 atagttaaag agatggcaat ggcaggtctg gatgagcata tgagacttgc caaaatggct
      841 gttgaagaaa cgggaagggg agtttatgaa gataaaacca taaaaaacat ttttgcaaca
      901 gagtatgttt accacagcat caaatatgat aaaaccgtcg gaataattaa tgagaatgag
      961 tttgaagact atgtcgaagt ggcggaaccg gttggggttg ttgcggctgt tacgccggtc
     1021 accaacccca catcaaccac catgtttaaa tccataatct ccatgaagac gagaaacccc
     1081 attatttttg catttcatcc gtcggctcaa aagtgcagtg cagaagctgc gagaatctta
     1141 agagatgccg ccattgaagc aggggctccc gaaggctgca tacagtggat tgagaaccct
     1201 tctattgagg caacccaagc acttatgcac catcccggcg ttgccatggt tcttgccacc
     1261 ggtggttcct caatggtaaa ggcagcctac agcaccggaa agcctgctct tggagtgggt
     1321 cccggaaatg ttccgtgtta tattgaaaag agcgtggatg taaaaagagc tgctaccgac
     1381 ctcattatgt caaagacctt tgacaatgga atgatatgtg catcggagca agccgtaata
     1441 gtagacaaag acatcagtaa agagttcgaa gaattcatga aggaaaacaa gtgttatttc
     1501 ttaaacgaag aagagataaa aaagcttgag aaaattgcaa ttgatgaagc taaatgtgca
     1561 atgagtccgg cggttgtcgg gcaatcggct tataagattg ctaaatcggc aggcttcgag
     1621 gttccggaag acacaaagat attaattaca aaattaaacg gtgtaggacc ggagtatcca
     1681 ttatcccgtg aaaagctgag tcctattctg gcatattata attctgacgg atgggagcat
     1741 ggaataagga ttgccgaaga aatggtggaa ttcggaggtc ttggacattc tgctgttatt
     1801 cattccaaaa atcagagggt aattgacgag ttttcaaaga gaatcaaggc aggaagaatt
     1861 attattaatt caccgtccac ccacggggca ataggagaca tatacaacac aaacatgcca
     1921 tccctcactt tgggctgcgg ctctttcgga agaaactcaa ctacagccaa tgtttctgct
     1981 gtaaacctga taaacaggaa gagagtcgca aggaggagag taaacatgca gtggtttaaa
     2041 atccccgaaa agatttattt tcagttcggt gccactcaat accttgaaaa aatgccgggt
     2101 atctcaaaag cctttgtagt taccgacccc tatatggtta aactggggta tgtcgacagg
     2161 gtattgtact ttttgagaaa gagacagcag tatgtgcatt gcgaaatatt cactgaggtt
     2221 gaacccgatc cttctctgga cacggtaatg aggggctatg accttatgaa caagttcaag
     2281 cccgacgtta taatagctct gggcggaggc tccgcaatcg atgccgccaa gggaatgtgg
     2341 ctctattatg aagaaccgga aacagatttt gcttcattaa ggcttaagtt tatggatata
     2401 agaaaaaggg tctttaagtt tccgaggctt gggaaaaaag ctaagtttgt ggcaattccc
     2461 acgacatcgg gcaccggttc cgaggtaaca tcctttgcag tcatcaccga taaagtcaac
     2521 aacaccaaat atccccttgc agactacgag ttaaccccgg atgttgccat aattgatccg
     2581 gaatttgtaa tgactgttcc tccttcagta accgcagaca caggaatgga tgtcctgact
     2641 catgccattg aggcgtatgt atcggttctg gcatcggatt tcacggacgc ccttgcaata
     2701 aaagccattc aactggtttt tgagtatctg ccccaagcat ataaaaacaa tagtaatgaa
     2761 cttgcaagag aaaaaatgca caatgcctct tgtattgcgg gtatggcctt taccaacgcc
     2821 tttttgggaa taaaccacag ccttgcacac aagctgggcg gcgaattcca tatacctcac
     2881 ggaagggcaa atgcaatact attgccccat gttatagaat acaatgcttc aaagcccagc
     2941 aagtttgttt ccttccccaa atatgagaca ttcattgccg acaagagata cgcagaaatt
     3001 gcaaaagctt tagggctccc tgcttcaact gtggaagagg gtataagaag ccttatcaaa
     3061 gcggttaagg atctaatgag agagctgaat attcctat
//
LOCUS       DAOOJC010000296         3083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_24454_length_3083_cov_3.513540, whole genome shotgun sequence.
ACCESSION   DAOOJC010000296 DAOOJC010000000
VERSION     DAOOJC010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24454_length_3083_cov_3.513540"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLA01_08005"
     CDS             <1..260
                     /locus_tag="PLA01_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HPD01274.1"
                     /translation="GTVWVWGNNDCGQLGIGSTDRRSVKPVMVEGLTGVKSIKADMES
                     TLAIKEDGSIWAWGKNDFGQLCNGTVDNCFSPVPVQVLLEN"
     gene            326..934
                     /locus_tag="PLA01_08010"
     CDS             326..934
                     /locus_tag="PLA01_08010"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPD01275.1"
                     /translation="MPKGLQNVFKSIHYNSPVILTMTFLSLGALILQLITHGHSTLLL
                     FSVYRSSPLDILSYFRLFGHVLGHLNWEHYINNFLLILLLGPMLEEKYGSKNMVFMII
                     ITALVTGLLNIILFPNVRLLGASGVVFMLILLSSFVNFKKGRIPLTFILVVVIYLGGE
                     VMNGLLARDNISQLTHLVGGICGSVFGFRWSERHQPSAKSNY"
     gene            958..1605
                     /locus_tag="PLA01_08015"
     CDS             958..1605
                     /locus_tag="PLA01_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPD01276.1"
                     /translation="MKKYKAVLFDFDYTLADSSKAVVDCISYSLRKMGYTVPTPDSIC
                     RTIGLTLSDAYRELSGDASESNAEIFRSYFKERADAVMKDDTRVFNTVENVLGRLKDK
                     GLKTGIVSTKYRYRIEDILERDGLLHYFDIIVGGEDVSAHKPDPEGLLRAIAALGITR
                     EDIIFVGDTVVDAKTAQNADVDFAAVLTGTTEQYEFSEFSTLAVLNDLSGLPDLI"
     gene            1811..>3083
                     /locus_tag="PLA01_08020"
     CDS             1811..>3083
                     /locus_tag="PLA01_08020"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HPD01277.1"
                     /translation="MSIKEMACQVKDASIKLAAADTKLKNDALAHIARSLIDRKDEIL
                     KANAMDLKRSEEENLSAPLLKRLRFDDAKIADVVNGINSLIGLKDPVGRTLLSTELDD
                     GLELYRVTCPIGVIGVIFESRPDALVQISTLCLKSGNGVLLKGGSEARETNCVLADII
                     SKATEEAGIPINWIKLLETRSDVNEMLKQDQYIDLIIPRGSNDFVRYIMDNSRIPVMG
                     HADGICHCYVDEDAQVDMAVRIVVDSKTQYVAVCNATETLLVHKNIAPKLLPELKEAL
                     DEKGVELFGCAQTQKIISVAPATEQDWKTEYLDYKLSIKIVENVDEAIDHINTYGSGH
                     TDSIVTDSRDKAARFMSLVDSGNVFWNCSTRFSDGFRYGFGAEVGISTSKIHARGPVG
                     LEGLLIYKYKLIGKGHIVEDYATRKKVFKHNN"
BASE COUNT          938 a          485 c          744 g          916 t
ORIGIN      
        1 acggaacagt atgggtatgg ggcaacaacg attgtggaca attaggaatc ggctcaacgg
       61 acagaaggtc tgttaaaccg gtcatggttg aaggccttac cggggtaaag tcgataaagg
      121 cggacatgga gagtacattg gcaataaaag aagacggatc catatgggct tggggaaaaa
      181 atgattttgg tcaattgtgt aatggaactg tggataactg tttttctcct gttcctgtgc
      241 aggtattatt agaaaattga ttattaagaa gctttttatc catattaaat aaacgcaccc
      301 attatatatg gggagggagg gtgttatgcc aaaaggttta cagaatgtat ttaaaagtat
      361 acattataat tcaccggtta tacttacaat gacattttta tcactaggag cattaatact
      421 tcaattaata acccacggtc attcgaccct gttattgttt tcggtgtacc gcagcagtcc
      481 gctggatatt ttatcctatt tcaggctttt cggacatgtg cttggccatt taaattggga
      541 gcactatata aacaattttc tgcttatact gcttctgggt ccaatgcttg aagaaaaata
      601 tgggtcaaag aatatggtgt ttatgattat aataactgcc ctggttacag gattgctaaa
      661 cattattctt tttccgaatg tccgtctttt aggagccagt ggcgttgtgt tcatgctgat
      721 attgctcagt tcctttgtca atttcaaaaa agggagaatt ccgctaacct ttatccttgt
      781 tgttgttatc tatctgggag gagaggttat gaacggtctt ttggcaaggg acaatatttc
      841 acagttaaca catcttgttg gaggtatctg cggaagtgtt ttcggtttca gatggtcaga
      901 aaggcaccag ccatcggcaa agagcaacta ttaaactgga ataactcggg gtgaataatg
      961 aaaaaataca aagcagtatt atttgatttt gattatactc tggcagattc atctaaagcg
     1021 gttgttgatt gtataagcta ttctcttcga aagatgggtt atactgtgcc aacaccggat
     1081 agcatatgca ggactatcgg tcttacactt tccgatgcct acagggaatt aagcggggat
     1141 gcatccgaat cgaatgcaga aatctttcgt agttatttta aggaaagagc ggatgcggtc
     1201 atgaaggacg atacgagagt attcaatact gttgagaacg tccttggaag attaaaggac
     1261 aagggactta agaccggtat tgtctctaca aaatacagat acaggataga agatattttg
     1321 gaaagggacg gacttttgca ttattttgat ataatagtgg gcggtgagga tgtatcggca
     1381 cacaaaccgg atcccgaagg cttgttaagg gctatagcag cattgggaat aacaagggaa
     1441 gatattatct ttgtgggcga cactgttgta gatgcaaaaa cggcacaaaa tgcagatgtt
     1501 gattttgccg cagttcttac cggtacaaca gaacagtatg agttttcgga atttagtacc
     1561 ctggcggtac taaacgactt aagcggattg cctgacttaa tttgaaagcg gatacatatt
     1621 aaaatgaaga aaaatgcgta acctgagttt acggagtatt tacacgctct agatttacgt
     1681 aacttaagtt tctggaatat ttttctttat acttatttaa cgcactcact tggttttgat
     1741 tgcagctgat tttgtgatat ggtataatta tgataattta ttacagaagc acttttgagg
     1801 agagatttat atgagtataa aggaaatggc atgtcaggtt aaggatgcat ccattaagct
     1861 ggcagcagcg gatacaaaac ttaaaaacga tgctctggct catattgcca gatcactaat
     1921 agataggaaa gatgagatac ttaaggcaaa tgctatggac ttaaaaagaa gcgaggagga
     1981 gaatctgtct gcaccgcttc tgaaaaggtt aaggtttgat gatgccaaga tagcggatgt
     2041 agtaaacggt ataaacagct tgataggttt aaaggatccg gtaggcagga ctcttctttc
     2101 aaccgagctt gatgatggtt tggaactgta cagggttaca tgccctattg gtgtaatcgg
     2161 tgttattttt gagtcacggc cggatgccct agttcaaatt tctacattat gtcttaaaag
     2221 cggaaatggt gttcttttaa agggtggttc ggaggcaagg gaaaccaatt gtgtacttgc
     2281 agatattatt tcaaaggcta cggaagaagc gggaattcct attaactgga ttaagctttt
     2341 agaaacaagg tccgatgtga atgagatgct taagcaggac cagtatattg atcttataat
     2401 tccaagagga tccaatgatt ttgtgagata tataatggac aattcgagaa ttccggttat
     2461 gggtcatgcg gacggtattt gccactgcta tgtagatgag gatgcacagg ttgatatggc
     2521 ggttcgtatt gttgtggatt ctaagaccca gtatgttgct gtttgtaatg cgacagaaac
     2581 cttattggtc cataagaata tagctccaaa gcttttaccg gagttaaaag aagctttgga
     2641 tgaaaaaggt gtggaacttt ttggttgtgc acagactcaa aagattattt ccgttgcccc
     2701 agctacagag caggattgga agacagagta tcttgattat aagctttcga taaaaatagt
     2761 cgagaatgta gatgaagcaa tagaccacat aaacacctat ggctccggac acaccgacag
     2821 tatagttaca gacagccggg acaaggcagc acgttttatg tctcttgtgg attcgggtaa
     2881 tgtgttctgg aactgttcaa ctcgattcag tgatggtttt cgctatggat ttggtgccga
     2941 agtgggtatc agtacgagta aaattcacgc aagaggaccg gtaggtcttg agggattgtt
     3001 gatttataaa tacaagttga ttggtaaagg ccatatagta gaggattatg caacaaggaa
     3061 aaaggtattc aaacacaata ata
//
LOCUS       DAOOJC010000297         3081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_24476_length_3081_cov_3.698942, whole genome shotgun sequence.
ACCESSION   DAOOJC010000297 DAOOJC010000000
VERSION     DAOOJC010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24476_length_3081_cov_3.698942"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1206
                     /gene="trpD"
                     /locus_tag="PLA01_08025"
     CDS             169..1206
                     /gene="trpD"
                     /locus_tag="PLA01_08025"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPD01278.1"
                     /translation="MLRKAITKLIEGKNLSEAEIVEALSCIMEGKATQAQIGSFITAL
                     RIKGETTEEITGCARVMREKAERISPNVEYYIDTCGTGGDGTNTFNISTAAALVAAAG
                     GVAVAKHGNRSVSSRSGSADVLEVLGANIDLSPIQVKECIEQVGIGFMYAPAFHKLMK
                     YAAGPRKELGIRTVFNILGPLTNPANARGQLLGVFNEKLTEPVANVLLNLGVERAMVI
                     HGMDGMDEITTTTATKVSEVRNNEVINYEINPEKYGFKLALKEDLTGKDAQENAQIIM
                     GIFNGDKGPRRDIVVLNSAAALYVGKVVSSIEEGIELAQEIIDSKKALKKLNEFLDMT
                     NTFLEREKLSS"
     gene            1420..2202
                     /gene="trpC"
                     /locus_tag="PLA01_08030"
     CDS             1420..2202
                     /gene="trpC"
                     /locus_tag="PLA01_08030"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HPD01279.1"
                     /translation="MILDKIVESKRMQLKEEMSKISIEGWKQRIKRPGLHKPLDFYSA
                     LKNKDSVSIIAEVKKASPSKGLIKVDFDPERIAKEYIDSSVQAISVLTEKNFFQGNEE
                     YIVKIRRFCPLPVLRKDFIIDLWQVYQSRCIGADAILLIVSLLSDEDLKKFQIVAGIL
                     GMQCLVEVHDEAELERALESGAKIIGINNRDLKTFEVDIKNTEKLLSHIPNDRVVVSE
                     SGIRSNEDIKYLYDLGVDAVLIGETFMRAQSIREKIKELRAV"
     gene            2205..2840
                     /locus_tag="PLA01_08035"
     CDS             2205..2840
                     /locus_tag="PLA01_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HPD01280.1"
                     /translation="MTGVKICGLRRKEDIEYVNRYMPQFIGFIFAESKRRVTKEWAKV
                     LRDELLPGIRAVGVFVNEDLEKVAQIVKYTGLDCVQLHGDETPEYIGQLMIVMKENKD
                     KKIEIWKGIRVKNKDSLRVMSEYSTDAFVLDTYVEGSYGGSGSVFDWHLAALANEYGK
                     IILAGGLNPQNVKEAVERVKPFVVDVSSGVETDGFKDEVKIKEFIRNVRQC"
BASE COUNT         1005 a          438 c          818 g          820 t
ORIGIN      
        1 aatattaagg aacttcctga atgtaacgag gcaataattg ttccataaaa ttttatttga
       61 accgaggtgt tataaagggg aaagaaaatt aataaaggtt ggcaataaaa gccgatagta
      121 actgggatct aaattaatag tcgagatgag aagagaggag agtttaaaat gttgagaaaa
      181 gcaattacaa agctgattga aggcaaaaac ttaagtgagg ctgagattgt tgaggcacta
      241 agctgtatta tggaaggaaa ggctacacag gcacagatag gcagctttat aacagcattg
      301 agaatcaaag gtgagaccac ggaagaaata accggttgtg ccagagtaat gagagaaaag
      361 gcagagagga ttagcccgaa tgtggaatac tatatcgata cctgtggcac aggaggggat
      421 ggtacaaaca ccttcaatat ttctactgca gcggcccttg ttgcagccgc agggggcgta
      481 gccgttgcaa agcacggtaa cagatcggtt tccagcagaa gcggcagtgc cgatgttttg
      541 gaggtcttag gagccaatat tgaccttagc cccatacagg taaaggagtg catagaacag
      601 gtgggcatag gctttatgta tgctcctgct ttccataaat taatgaagta tgcagccgga
      661 ccgagaaagg aactgggaat aaggacggtg ttcaatatac tggggccttt gaccaatccg
      721 gcaaacgcca ggggacagct tttgggagta tttaatgaaa aacttacgga gcctgtggcg
      781 aatgtgctgt tgaatttggg agttgaaagg gctatggtaa tacatggcat ggatggaatg
      841 gacgaaataa ccacgactac cgccactaaa gtttcggaag tgcggaacaa cgaggttatc
      901 aattatgaaa taaatcctga gaaatatggc tttaagcttg ccttaaaaga ggatttgaca
      961 ggaaaggatg cccaggagaa tgcacaaata attatgggaa tctttaacgg tgataagggc
     1021 cccaggaggg acattgttgt actgaattcc gcggcggcat tgtatgtggg aaaggttgtt
     1081 tcctctattg aagagggaat tgaacttgca caggaaataa tcgacagcaa aaaggcatta
     1141 aagaaactaa atgaattttt agatatgacc aacacgtttt tagagcgtga aaagttgtcg
     1201 agttgattat tggattagtg aaataaaaat aaaatgtcaa ggcggtttgg ctgttgtgat
     1261 atagtaaaga gctgttgagt cggttacatg gctgaaatga tctagtgaag ggctgctgag
     1321 ccggttacat ggctgttgtg atatagtgaa gagctgttga gccggttaca tggttattat
     1381 gatactttga taaattttcg agccgattgg agggctaaga tgatactgga taaaattgtt
     1441 gaaagcaaaa ggatgcaact taaagaagaa atgagtaaaa tttctataga gggttggaag
     1501 caaagaataa aaagaccggg acttcacaag ccgttggact tttattcggc ccttaaaaac
     1561 aaggattctg tgtccattat agcggaagta aaaaaagcat caccgtcaaa gggacttata
     1621 aaagtggatt ttgatcctga aagaattgct aaggagtata tagattcaag tgttcaggcc
     1681 atttctgtcc tcactgagaa gaattttttt caaggtaatg aagagtacat tgtaaaaata
     1741 agacggtttt gcccgctgcc cgttttgaga aaggatttta taattgatct ttggcaagta
     1801 taccaatccc ggtgcatagg ggcggacgcc atattgctga ttgtttctct tcttagcgat
     1861 gaggatttga agaagttcca gatagttgct ggtatacttg gtatgcagtg tctggtggag
     1921 gttcacgacg aagccgagct tgaaagggcc ttggaatccg gtgctaaaat tatcggaatt
     1981 aacaacagag accttaaaac ctttgaggtg gatattaaaa acacagaaaa gcttctttcc
     2041 catataccca atgaccgggt tgtggtcagt gaaagcggaa ttcgaagtaa tgaagacata
     2101 aaatacttgt atgatcttgg tgtggatgcc gtattaatag gggaaacctt tatgcgagct
     2161 caatccataa gggagaaaat aaaggagtta agagcggtgt gattatgaca ggggttaaga
     2221 tatgcggttt gagaagaaaa gaggatatag agtatgtgaa ccggtacatg cctcagttta
     2281 ttgggtttat atttgctgaa agcaagcgta gggttacgaa ggaatgggcg aaggtgttaa
     2341 gggatgagct gctgcccggt attagggctg tgggagtgtt tgtgaacgag gatttggaga
     2401 aagtggcaca gatagtgaaa tatacaggac tagactgtgt tcagctccat ggtgacgaga
     2461 cgccggaata tattgggcag cttatgatag ttatgaaaga aaataaggat aaaaagattg
     2521 aaatatggaa gggtataagg gttaaaaata aagattccct aagggtaatg tccgaataca
     2581 gtacggatgc ttttgtcctt gatacttatg ttgaaggaag ctatggcggt tccggcagtg
     2641 tatttgactg gcatcttgcc gctcttgcca atgaatacgg gaaaattatc ctagccggag
     2701 gtctgaatcc gcaaaatgtg aaggaagcgg tggagagggt aaagcccttt gttgtggatg
     2761 taagcagcgg tgttgagacc gatggtttta aggatgaggt caaaataaag gagtttatta
     2821 gaaatgttcg gcaatgttaa agcaacatta ccgaaatatt atatatgaga ctgtcagaac
     2881 cagtgtgtat aattaaattc tttctgacag cttttttatt aaattctgaa aaggacttta
     2941 gtttatggtt ccggtcaaga aataatttga aatatggaga tattttgaga ttcgttaaga
     3001 taatccgttt caacggttat ttatttaagc gtgaaggtca aagaaagtca aagtctaatg
     3061 ttgatgaata attagaaaaa t
//
LOCUS       DAOOJC010000298         3073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_24570_length_3073_cov_3.978794, whole genome shotgun sequence.
ACCESSION   DAOOJC010000298 DAOOJC010000000
VERSION     DAOOJC010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24570_length_3073_cov_3.978794"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2167
                     /gene="gyrA"
                     /locus_tag="PLA01_08040"
     CDS             <1..2167
                     /gene="gyrA"
                     /locus_tag="PLA01_08040"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPD01281.1"
                     /translation="GSIDGDPPAAMRYTEARLSKISLEMLRDINKDTVDFKPNFDEHD
                     FEPVVLPSRFPNLLVNGSSGIAVGMATNIPPHNLVEVIDGIVAVIDNPEMTIDEIIKI
                     IKGPDFPTGATIVGTQGIKNAYRTGRGKIVLRAVTNIEQMTANKQRIVVTELPYQVNK
                     ARLIEKIADLVKEKRIESISDLRDESDRDGMRIVIELKKEANANVVLNQLYKNTQMQD
                     AFNVNMVALVQTPDKKFEPRVLNLRQAMDYYIEHQKDVIVRRTKYELDKAEARAHILE
                     GLKIAIDNLDEVIKIIRGSKNETTAKERLMERFSFSDKQAQAIVDMRLGRLTALEREK
                     LEKEYQELLEKIKYYKDVLANEFMVLNIVKDELNEIKEKYGDERKTKIVPDESDIDIE
                     DLIDDEETVVTLTHFGYIKRLPADTYKSQKRGGKGITGLSTREEDFVENLFVTRTHDY
                     ILFFTSKGKVYRLKAYQIPEAGRQAKGTAIVNLLELDQDEKITTVIPISEYKEGLYLF
                     LSTKKGIVKKTDLMDYDNIRKGGLIAVSLRENDELIDVQLTDGTKDILLVTRNGLSIR
                     FNEQDARPLGRVSQGVRGIKLEDDDEVIGMSAYIENTSLLVVTENGFGKRTELDEYKV
                     QFRGGKGILTYRVTDRTGKLVGMKLVCDDDDVMLISSDGTIIRIKVRDISVLGRATQG
                     VTLMRMDEGVKVMAVARIVNEDDDEVEDSNHCNDENEEK"
     gene            2667..>3073
                     /locus_tag="PLA01_08045"
     CDS             2667..>3073
                     /locus_tag="PLA01_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPD01282.1"
                     /translation="MEKRKRIISYLLIICMIISSLSCVAAGTEVQTDSNQNISVVSSN
                     NSQTFPDIKDHWCKEYIEKFLSKKWVVGYDDGLFRPDRFVTRAEFTAMVVNIFKKVES
                     VNECNFTDVEKSDWFYNAVAYAAAEGLIQGYENG"
BASE COUNT         1068 a          433 c          743 g          829 t
ORIGIN      
        1 cggttccatt gacggggatc caccggcggc tatgaggtat actgaggcaa gattatcaaa
       61 aatatctctg gagatgctaa gggacatcaa caaggatacc gttgatttta aaccgaactt
      121 tgatgagcat gattttgaac ccgtagtatt gccgtcaaga tttccgaatc ttttggtcaa
      181 tggttcttca ggtattgccg ttggtatggc aacaaatatt cctcctcata atctggttga
      241 ggttattgat ggaatagtag cggtgataga caatccggaa atgactattg atgagattat
      301 taaaataatt aaaggccctg attttcctac tggtgctaca atagtcggaa cacagggtat
      361 caagaatgcc tatagaaccg gcaggggaaa aatagtgctg cgggctgtta ccaatattga
      421 gcaaatgacg gccaacaagc agagaattgt tgtaacagag cttccctacc aggttaacaa
      481 ggctagatta attgagaaaa tagcagatct ggttaaggaa aagagaatag agagcatatc
      541 ggatttgagg gatgagtctg acagagacgg aatgagaata gttatagagc ttaagaaaga
      601 agccaatgca aacgttgttt taaaccagct ctataaaaac actcaaatgc aggatgcttt
      661 taatgttaac atggtagcac ttgttcagac tcctgataaa aagtttgaac cccgcgtact
      721 taatttgagg caggctatgg actattatat cgaacaccag aaggatgtaa tagtcagaag
      781 gacaaaatat gagcttgata aagctgaggc aagggctcat attttggaag gtttgaaaat
      841 tgcaatagat aaccttgatg aagtaataaa gattataagg ggatccaaaa acgagaccac
      901 agctaaggaa aggttgatgg agaggttctc tttcagtgac aaacaggcac aagccattgt
      961 agatatgagg ctgggaaggc ttactgcact ggaaagagaa aagcttgaaa aagagtatca
     1021 ggagcttttg gaaaagatta aatactacaa agatgttctt gcaaatgagt ttatggtttt
     1081 aaatattgtt aaggacgaac ttaacgagat aaaggaaaag tacggggatg aaagaaaaac
     1141 taagattgtt cccgatgaaa gcgatataga tatagaagat ctgatcgatg atgaggagac
     1201 tgttgtaacc cttactcatt tcgggtatat aaagaggctc ccggcggata cttataagag
     1261 ccagaagaga ggcggcaaag gtataacggg tcttagtaca agagaagaag actttgttga
     1321 aaaccttttt gttaccagga cccatgacta tattcttttc tttacgagta aaggaaaggt
     1381 atataggctg aaggcttatc aaatacccga ggcaggacgc caggcgaaag gaacggcaat
     1441 agtaaacctg cttgaacttg atcaggatga aaagattaca accgtgattc cgataagtga
     1501 gtataaggaa ggattgtatc tgttcctttc gacaaaaaag ggtatagtta agaaaacgga
     1561 ccttatggac tatgataaca tcagaaaggg cggacttatt gcagtttcgc tgagggaaaa
     1621 cgatgagcta attgatgtgc agttaaccga cggaacaaag gatatattac tggtaacaag
     1681 aaacggattg tccataaggt ttaacgagca ggatgcaagg ccgttaggca gggtgtccca
     1741 gggagtaagg ggcattaagc ttgaagacga tgacgaagta atcggcatga gtgcatatat
     1801 tgaaaatacc agtcttctgg tggttacgga aaacggtttc ggtaagagga cggagcttga
     1861 cgaatataag gtgcagttca ggggaggaaa aggcatcctc acatacaggg taacggacag
     1921 gaccggaaag ctggtgggaa tgaagctggt atgtgatgat gatgatgtga tgctcataag
     1981 ctcggatggt accatcataa ggatcaaggt acgggacata agtgttctgg gcagggcaac
     2041 acagggcgtt acccttatga gaatggatga gggagttaag gttatggctg ttgcaagaat
     2101 tgtcaacgaa gatgatgatg aagtggaaga cagcaatcat tgcaatgatg agaatgaaga
     2161 gaagtagttg gataaagcaa tatatgcccg tatagagaaa ggagacgtga aaaagcgtct
     2221 ccttttggta aagatgtttt aaagcatatt atagattata atttactttc tatgtaaaaa
     2281 cattattttt attgagagga gctaagcaaa ataatgttaa aacaaggctc tttttttata
     2341 caggttattt gcatattgat gagcagcatt tcttatgcag cttcggatat taatacgcaa
     2401 tccgatatca attaaacaaa tatacgtgaa caaatttgtt gtagaaaaaa aactctttac
     2461 agtttgaaaa atatagggtt ttattatata attaataaaa tagtatagta aattatgttt
     2521 acttatattg gatgcagaag aataaatata ttcatggata tgcattcaat atatttatta
     2581 tatagtttcg taaaattatg agtaaatcgt tgtaattttt ttacgaaacg tatggaatta
     2641 tattacttta gggggattaa aaaataatgg agaaaagaaa aaggattatt tcttatttac
     2701 ttattatctg tatgataata agcagccttt catgtgtagc tgcgggtaca gaagttcaaa
     2761 ccgatagtaa tcaaaatata tcggttgtca gcagcaacaa ttcacaaact tttccggaca
     2821 ttaaagacca ttggtgcaaa gaatatattg aaaaattctt gagcaaaaaa tgggttgtag
     2881 gctatgatga cggactattt cgtcccgata ggtttgtaac ccgggcagag tttactgcaa
     2941 tggtagtgaa catttttaag aaagtagaaa gtgtaaatga atgcaatttt accgatgtgg
     3001 aaaagagcga ttggttttat aatgccgtgg cttatgctgc tgctgaaggc ttaatacagg
     3061 gatatgaaaa cgg
//
LOCUS       DAOOJC010000299         3072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_24583_length_3072_cov_3.185283, whole genome shotgun sequence.
ACCESSION   DAOOJC010000299 DAOOJC010000000
VERSION     DAOOJC010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24583_length_3072_cov_3.185283"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1876
                     /gene="fliD"
                     /locus_tag="PLA01_08050"
     CDS             <1..1876
                     /gene="fliD"
                     /locus_tag="PLA01_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HPD01283.1"
                     /translation="TVSDVSNTFVSSLGFSDGQKNFITGKDVNTSFSVYTNGSFKITV
                     GNGTEQTINIDKAKDINELTSKIQAAINENTQLKGKLRVTNDGSKLSFIAISTETIKL
                     TSADKNNILDKLGFSDKTSISAVTSRAIDLSGNEKGKTFIVIINGEDKIIEIDKDYSD
                     LSALASYIKGQLGGAVNVLKASDGNRLIFTTTGTNMLTIKKGPDDGLEMLGFSAQDNR
                     SNKVSLSSKLDSIKTSFASDLNIEDPEANVVFTINGQTIDVGKSYAKATLGDVVNAIN
                     NSSAGVKVTYDSLRDRFVMESKTQGATSSISYTDTDPENGLLKAMGLVDGTYITGTDA
                     EFSLDGVTGMKRSSNEFTIDGVTYTLKGISSEPVTVEIGQDIDAVVEKIKSFVENYNE
                     VLDLINGLLSEKRDRDYLPLTDSQKEQLEEDEIKKWEEKAKAGLLSNDSILQSIVSSM
                     RTALYDKVEGTSLSLYQIGISTGSYKDKGKLKIDEAKLREALQDNFQEVVKLFTNKSQ
                     YSYDESLSDLEKRQTRYRESGLAQRLSDILQDNIRTVRDSNGKKGKLLEKAGMSGDLS
                     EFNNLMNSEINNKNKLIDTLIEKLFEKEDYYYKKFAAMEKILSEMQNQSNWLMSQLGR
                     "
     gene            1932..2339
                     /gene="fliS"
                     /locus_tag="PLA01_08055"
     CDS             1932..2339
                     /gene="fliS"
                     /locus_tag="PLA01_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HPD01284.1"
                     /translation="MSLNNAYNQYKENSVHTATPEEQTLMLYNGLVKYLMQAQMAVNG
                     NKIEKAGTCIIKAQDIISEFRSTLDMKYDISHQLEQLYDYMYRRLVDANIKKDIGIIE
                     EILGFAKELRDTWEKAIKIARQQQSTTKAQVAR"
     gene            2351..2839
                     /gene="flgN"
                     /locus_tag="PLA01_08060"
     CDS             2351..2839
                     /gene="flgN"
                     /locus_tag="PLA01_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HPD01285.1"
                     /translation="MDITPEVYIERLIDISSKKDSCLNEILRLTMAQSEAIKEDGLEV
                     LQKLIDDKQRKIEEINKADEDFNVYLTRLKQKLGISRLDEINNPGIKGVKELQEIISR
                     IIKLVGKISEIENQNIERANNLLNDFGSNIKRINEGKRANNAYNNAYSLNAPSYYIDK
                     KK"
BASE COUNT         1119 a          462 c          707 g          784 t
ORIGIN      
        1 aactgtcagt gatgtaagca acacctttgt ttcatcttta ggtttttcgg atgggcagaa
       61 aaactttatt acaggtaagg acgtaaatac cagtttttcc gtatatacaa acggaagctt
      121 taaaattacc gttggtaacg gaacggagca gacgataaac attgataagg caaaggatat
      181 taatgaactt accagtaaaa ttcaggctgc tattaatgaa aatactcaac tgaaaggtaa
      241 gctgcgtgtg actaacgacg gaagcaaact gagttttata gccatatcga cagaaacaat
      301 aaaattgaca tcggcagaca aaaacaacat cttggataag ctgggctttt ccgacaagac
      361 aagcataagt gcagtaacct caagagctat agatttgagc ggcaacgaaa agggaaaaac
      421 ctttattgtt attattaacg gagaagacaa aatcatcgaa atagataagg attacagtga
      481 cttaagtgcc ctggcttctt atataaaggg gcaactcggc ggtgctgtga atgttttgaa
      541 ggcttctgac ggtaacaggc tgatttttac aaccaccgga acaaacatgt tgacaatcaa
      601 aaaaggtccg gatgacggat tggagatgct tggttttagt gcacaagaca acaggagcaa
      661 taaggtttct ctgtcttcaa agcttgacag tataaagact agctttgcaa gcgatttaaa
      721 tattgaggat ccggaagcca atgtggtatt taccataaac gggcagacta ttgatgtggg
      781 taagtcatat gccaaggcaa cccttggaga tgttgtaaat gcaattaata acagcagtgc
      841 aggggtcaag gtaacctatg attctttaag ggacagattc gtgatggaat caaagacgca
      901 aggggctacc tcttcaatat catataccga caccgatccg gaaaatgggt tgcttaaggc
      961 gatgggactg gttgacggaa cttatataac cgggacggat gccgagtttt ccctcgatgg
     1021 agttacagga atgaagagaa gcagcaatga atttactata gatggggtga cttatacatt
     1081 aaagggtatc tcttcagaac cggtaactgt ggagatcgga caagatattg atgcagttgt
     1141 tgaaaaaata aagagttttg ttgaaaatta taatgaagta cttgatttga taaacggctt
     1201 gctgtcggaa aaaagggaca gggattattt acctctcacc gattcccaga aagaacaact
     1261 cgaagaagac gaaataaaga aatgggagga gaaagctaaa gccggactgc ttagtaatga
     1321 cagcatattg cagagcattg tatccagtat gagaacagcc ttatacgata aggtggaggg
     1381 gacttcgtta agcctttatc aaataggaat ttcaacaggg tcatataagg ataaaggaaa
     1441 gctaaaaata gacgaggcaa agcttagaga agcattgcag gataatttcc aggaagtagt
     1501 aaaattattc accaataaat cccagtactc atacgacgaa tccttaagtg atctggagaa
     1561 gagacaaaca aggtacagag agtccggatt ggcacagaga ctcagtgaca tacttcagga
     1621 taatataaga actgtccgcg actcaaacgg caaaaagggt aaactgcttg aaaaggcagg
     1681 catgtccgga gatttatcgg aatttaacaa cctgatgaac agtgagatta ataataaaaa
     1741 caaattgatt gatacattga tagaaaagct gttcgaaaag gaggattatt actacaaaaa
     1801 gtttgcagcc atggaaaaaa tactaagcga gatgcagaac cagtcgaact ggttgatgtc
     1861 gcagttgggt agataacgaa actgcacttt gttgaaaaaa tcgatcacaa aaccattgga
     1921 gggattattt aatgtcatta aacaatgcat acaatcagta taaggaaaac tcagtacata
     1981 ctgcaactcc ggaagagcag acattaatgc tttataacgg gcttgtgaag tatttaatgc
     2041 aggctcaaat ggctgtaaat ggaaataaaa tcgagaaggc tggaacctgt ataattaagg
     2101 ctcaggatat tatttccgaa ttccggtcta ctcttgatat gaaatatgat atttcgcatc
     2161 agcttgaaca attatatgat tatatgtata ggagacttgt tgatgccaat attaaaaagg
     2221 atatcggcat aattgaagaa atattgggat tcgctaaaga gctcagggat acctgggaaa
     2281 aagcaataaa gattgcaaga cagcagcaaa gtacaacaaa agcacaggtg gccagatagg
     2341 agagattata atggatatta ctccggaggt ttatattgaa aggcttattg atatttcaag
     2401 caaaaaggac agttgtttaa atgaaatact gcgtttaacg atggcccaat cggaagcaat
     2461 taaggaagac gggctggagg ttcttcaaaa gctgatagat gacaagcagc gtaaaatcga
     2521 ggaaattaac aaggcggatg aggactttaa tgtgtatttg acaagactga agcaaaagct
     2581 tggaattagc agactggacg aaatcaataa tccgggaata aagggcgtga aggagcttca
     2641 ggagattatt tcacggataa ttaagcttgt agggaaaata agcgaaattg aaaatcagaa
     2701 cattgaaagg gcaaataacc tcttgaatga ttttggaagc aacatcaaaa gaataaacga
     2761 aggtaaaagg gcaaacaatg cctataataa tgcttattca ctgaatgctc cttcttatta
     2821 tatcgataaa aagaagtagc ttttaactag aaaaggaaaa tatactgaca aagcatagct
     2881 atattgagct atgctttttt atgtattcac ttttattcat gaaagagggg taagctaata
     2941 tatttttggt tatatgaaaa aataaattat ggtgctgtta attgacggaa ttattgtgta
     3001 taataatagt gcactttacg aataagggct ggtggcattt ggaatttata tgactggaag
     3061 tggtatgtat ga
//
LOCUS       DAOOJC010000300         3068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_24638_length_3068_cov_3.659476, whole genome shotgun sequence.
ACCESSION   DAOOJC010000300 DAOOJC010000000
VERSION     DAOOJC010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24638_length_3068_cov_3.659476"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..673
                     /locus_tag="PLA01_08065"
     CDS             221..673
                     /locus_tag="PLA01_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HPD01286.1"
                     /translation="MKIKEGINSPTGMPEVAGSNGRRVADSSGTSFKSHLQQLNDLTH
                     EEKIRFLVSQIEEQGQKLSKRIDVKELKIYKRLISEFLDEALNNSHLFSKDSRLDRRG
                     RYKVYATVKKVNQHLEELTKQVLSAEEDNIKILQRIEDIRGLILDLIL"
     gene            782..1771
                     /locus_tag="PLA01_08070"
     CDS             782..1771
                     /locus_tag="PLA01_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HPD01287.1"
                     /translation="MDFSGIIGQKDVVDALKASLINNRVGHAYIFSGPCGIGKKTAAH
                     IFSSILMCNEGKADESCGKCKSCLLFENGSNPDYCVLEVEDSSIGVEDIRKLQHDISV
                     RPLYSKKKVYLIPEAGKMTVQAQNCLLKTLEEPPEYGVIILVTSNYDALLETIRSRSV
                     RYDLKRNTFDEVKGFLTDKFGIEYGEAGFVSSYANGVIGRAIELLESEDLKKARDRVI
                     DALAKLNRAQRDAVNEINALFEESKDDAESILDIMLLFYRDLLVARKYGNENILINSD
                     KKDIILENAQGFSAGKLLNNIAVIEDTRKVIKKNANFQLAIEVMLMKLQEENG"
     gene            1771..2643
                     /locus_tag="PLA01_08075"
     CDS             1771..2643
                     /locus_tag="PLA01_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HPD01288.1"
                     /translation="MVKVVGVRFKKAGKIYYFDPGDLVIDLHENVIVETARGIEFGLV
                     VVPNTEISEDEVVAPLKKVIRVATEEDKAHHAENIAKEKDAFNICLQKIQDHNLEMKL
                     IDVEYTFDNNKVLFYFTADGRVDFRELVKDLASVFRTRIELRQIGVRDEAKMMGGLGV
                     CGRVLCCNSFLGDFQPVSIKMAKEQGLSLNPTKISGACGRLMCCLKYEQDAYEEILTR
                     VPNEKAIVETPDGQGVVMGISLLKELIKVKLDKDNEADLKIYHVSEVKVIKDVSRDEI
                     DEEIDLEELKQLED"
     gene            2782..2952
                     /locus_tag="PLA01_08080"
     CDS             2782..2952
                     /locus_tag="PLA01_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPD01289.1"
                     /translation="MAYFITDSCISCGTCESECPVSCISAGDSVYVIDADACIECGAC
                     ANVCPVDAPQPE"
BASE COUNT          984 a          429 c          785 g          870 t
ORIGIN      
        1 tcatgagagt atataccggg ctgtaaaaga aacaatttaa aggaacggct gtttgaattt
       61 atccgttatt gccgatataa tgtatgtaat actattcagt ggtttggact ttatatggaa
      121 aagcagcaac ttcatctgcg gcaaacgaaa taacaatttg gcataataca ggtactgcgg
      181 gatgttaaac gtgattaatg cgatattgga gtgattaacc gtgaagatta aggaaggtat
      241 taacagccca acagggatgc cggaggttgc cggcagcaat ggcaggaggg tggcggacag
      301 cagtggaaca agctttaaaa gccatttgca gcaattgaac gatctcaccc atgaggaaaa
      361 aattcgcttt ttggtaagcc agattgagga acaggggcag aaactgagta aaaggataga
      421 tgtgaaggaa ctgaagatat ataaacgtct tatatcagaa tttctcgatg aagcattgaa
      481 caactcgcac ctgttttcaa aggacagccg ccttgacagg agaggcaggt ataaggtgta
      541 tgcaacagta aaaaaggtta atcagcattt ggaagagctt acaaagcaag tgctttccgc
      601 tgaggaggat aatatcaaga ttttgcagag gattgaggat ataaggggtt tgattttgga
      661 ccttatacta tagcggtttg tgcatgaatg ctttaataaa gttgttttgt attgaatttt
      721 tacaaaaaga cagttggaat ttaaaaaata tgtacaattt gcggacttta ggggtgtagc
      781 ggtggacttt agtggaatta tcggtcaaaa agatgttgtt gatgcactta aagcatcatt
      841 aataaacaac agggttggac atgcatatat attttccgga ccttgtggaa taggaaaaaa
      901 gacggccgca catattttct catcgatatt aatgtgcaat gaggggaaag cggatgaaag
      961 ctgcggaaag tgcaaatcct gccttctttt tgaaaacgga tccaatcccg attattgcgt
     1021 tttggaagtt gaggactcaa gtataggtgt tgaagatata aggaagctgc agcatgatat
     1081 atccgtaagg cctctctatt caaaaaagaa ggtgtattta attccggagg cggggaagat
     1141 gaccgttcag gcccagaact gtttgcttaa aacactggaa gagccgccgg agtatggggt
     1201 tattattctg gttacttcga actatgatgc ccttttggag accatacgtt caagaagtgt
     1261 aagatacgac ttaaaaagaa atacttttga tgaggtgaaa gggtttttaa cggataaatt
     1321 tgggatagaa tatggcgaag cgggttttgt gtcatcctat gcaaacggcg taataggaag
     1381 agccattgaa ctgttggaat cggaggattt aaaaaaggcc agggacaggg tgattgatgc
     1441 actggcaaag cttaataggg cacaacgtga tgcagtaaat gaaatcaatg ctttgtttga
     1501 ggagagtaag gatgatgcgg aatccatact ggatattatg cttttgtttt acagggattt
     1561 gcttgttgcc aggaagtatg gaaatgaaaa tatattgatt aattccgata aaaaggatat
     1621 tatattagag aatgcacagg ggttctctgc tggcaaactt ttgaacaaca tagccgtaat
     1681 tgaggatacc cgtaaggtca taaaaaagaa tgcgaacttt cagcttgcta tagaggttat
     1741 gcttatgaaa cttcaggagg aaaatgggta atggtaaagg tggttggagt aagatttaaa
     1801 aaagccggga aaatatatta ttttgacccc ggtgatttgg ttatagattt acacgaaaat
     1861 gttattgtag agactgccag aggtatagaa ttcggactgg ttgttgtccc caatactgaa
     1921 atcagtgagg atgaagtagt tgctccttta aagaaggtta taagagttgc cacagaagaa
     1981 gacaaggccc atcatgcaga gaatattgca aaagaaaaag atgcgtttaa tatctgtctt
     2041 cagaagattc aggaccataa cttggaaatg aagcttatag atgtggaata tacctttgat
     2101 aataacaagg ttttgtttta tttcactgcg gacggcagag tagatttcag agaattggtg
     2161 aaagaccttg cttccgtttt caggacgagg atagagctta gacagattgg tgtcagggac
     2221 gaggctaaaa tgatgggtgg tctgggagtt tgcggccgtg ttctttgctg taattctttt
     2281 ttgggtgatt tccagccggt gtctataaag atggcaaagg agcaggggct ttcgttaaat
     2341 cctacaaaga tttccggtgc ctgcggaagg cttatgtgtt gcctgaaata tgagcaggat
     2401 gcttacgaag aaatacttac aagggttcct aatgagaaag ccattgttga aacacctgac
     2461 ggacaaggag tagtaatggg aataagcctt ttgaaggaat tgataaaggt aaaactggac
     2521 aaggacaatg aggccgatct caagatctat catgtaagtg aggtaaaagt tattaaggat
     2581 gtaagccgtg atgagataga tgaagaaata gatttggaag agttaaaaca gctggaggat
     2641 taagatttat aaataatatg tcaaagaatt atgaaaaaat ggttttcttt tgatgggttt
     2701 agtgataaaa ttaattagtc aatagtttct attgatttta taagcttact atggcaaatc
     2761 tatttaggag gtgtacctta aatggcatat tttataactg actcttgtat tagctgcggt
     2821 acctgtgaat cagagtgccc ggtatcttgt atatcagcag gggacagcgt ttacgttata
     2881 gatgctgatg cttgtataga atgcggagcg tgtgcaaacg tttgtcctgt agatgctcca
     2941 caaccggaat aatagggttg cataaggggg ctggataggt atatttttat atactttatt
     3001 tggccctttt ttagtgggat ttcacgaagt gaaatgacca aaaagcgaaa gggttcgatg
     3061 cccttgag
//
LOCUS       DAOOJC010000301         3061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_24711_length_3061_cov_2.645709, whole genome shotgun sequence.
ACCESSION   DAOOJC010000301 DAOOJC010000000
VERSION     DAOOJC010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24711_length_3061_cov_2.645709"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="PLA01_08085"
     CDS             complement(<1..1236)
                     /locus_tag="PLA01_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HPD01290.1"
                     /translation="MYDSRKNRFRINGVPKVLLIIFLSVAAIKLGMLGGDILFRCDKK
                     IIRNVDIENYRSNLKTTLPIIDTIYNSGNISVSLTGYMKDVVQGIFHFDMNSPATILG
                     AQSPYYYSYYIGGYQKHLADNGSSKPHFYIAEIGNNVRKDNVQDSTKEAAPEPTYPAS
                     SISYSVDEDDRKGTPEKNTVTYNEIAINNNDTNYEIDIKELMSEPLNLSFDKKGPKIL
                     IYHTHTNEGYLKDISELEKDGVPSRTTDARYSVVRVGEELANQFRKKYGIEVIHNATM
                     HNYPSDTGCYARSLNTATSILKSYPSIKIVLDIHRDGIGQKKLRVATKINNKSVAGIM
                     FVVGTDTTGLEHPNWRENLKFAITLQQKLNEKYPGITRPIYISYNRYNQHLTPGALIV
                     EIGGDGNTIDECLESAKYLA"
     gene            1625..1849
                     /locus_tag="PLA01_08090"
     CDS             1625..1849
                     /locus_tag="PLA01_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01291.1"
                     /translation="MYSFKTLFCYNCKSVLLNLPEVEISKLNGLNFVCECCGHRNLLT
                     KNQFFKSVDTYDPYISIQSVDNIISERALY"
     gene            complement(2044..>3061)
                     /locus_tag="PLA01_08095"
     CDS             complement(2044..>3061)
                     /locus_tag="PLA01_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HPD01292.1"
                     /translation="RATNYIGFIRKALKDAGLSHIPVLSLNFAGLEKNPGFKIRPGLV
                     NKALMAVVYGDLLMRVLYRVRPYERISGSANLLYDSWVPRCLESIRTGSQRTFRENIY
                     RIVEDFDNLDVYNVKKPRVGLVGEILVKFHPQANNNVVDIIEREGAEAVMPDLTDFLL
                     YCAYNENFRYTHLSGKKINRTIKNLAISFIEYYRRDMKKALAQSKRFSPPCSISELAE
                     MASAILSVGNQTGEGWFLTAEMIELIKSGVQNIVCMQPFACLPNHVTGKGMIKELRRR
                     YPDSNIVAVDYDPGASEVNQLNRIKLMLSVAFKNFYKAEQDLQKKDSIYDSESTGEIV
                     KTLV"
BASE COUNT          846 a          635 c          516 g         1064 t
ORIGIN      
        1 agccagatat tttgcacttt cgagacattc atctatggtg tttccgtccc cgcctatttc
       61 cacgattaat gcccccggag tcaaatgctg gttataccgg ttatagctga tatatatcgg
      121 tcttgtaatc ccaggatatt tttcattcag tttttgctgg agggttatgg caaacttaag
      181 gttttccctc caattgggat gttcaaggcc tgtggtgtcc gttcccacta caaacattat
      241 cccggcaacg ctcttgttgt ttattttggt cgcaaccctc agtttcttct gccctatacc
      301 gtccctatga atatccagga caattttaat tgagggatag ctttttaaaa tactggtggc
      361 agtgtttaaa gaccttgcat agcagccggt gtctgaggga taattatgca tggtggcatt
      421 gtgaattact tcaataccgt atttttttct aaattgattg gcaagctctt cacccaccct
      481 tacaacgctg tatctggcat ccgtggtcct gctgggaacg ccgtcttttt caagttctga
      541 aatgtctttt agatatcctt cattggtatg ggtatgataa atcaatattt tgggaccttt
      601 tttatcaaag ctgagattta agggctcgct cataagctct tttatatcaa tctcataatt
      661 agtgtcatta ttgtttattg ctatttcatt atatgtaaca gtgttttttt caggagttcc
      721 ttttctatcg tcctcatcca cggagtaaga aatactgctg gccgggtagg tgggctcagg
      781 tgctgcctct ttagtactat cctgtacatt atcttttcta acattattgc caatttctgc
      841 aatataaaaa tgaggcttgc tgctcccgtt atcggcaaga tgtttctggt agccgccaat
      901 ataatagctg taatagtaag gtgattgagc acctaaaatt gtagcgggac tgttcatgtc
      961 aaaatgaaaa attccctgta caacatcctt catatacccg gttagagaaa cacttatatt
     1021 gccgctgtta taaatagtat ctattatcgg caaagtagtc ttcagattgg acctataatt
     1081 ttcaatatca acgtttctta taattttctt atcacatctg aatagtatat cccctccgag
     1141 catacccagt tttattgctg ccaccgacag aaaaattatc agaagcacct taggaactcc
     1201 attaatccta aacctgtttt tccttgaatc gtacattttc attttatcct ccgttaagat
     1261 tcatgtaatt tataaaatcc cctttgtttt catttccagg ggatttcttt gtattgatta
     1321 tattcatgat tcttttttaa aatgataact attgatttta ttcctaaaaa aatatataat
     1381 ataaatgcag tatgtgaata tgtaaacaga atgtttcttt tcttgatttt atcttataat
     1441 tcgcaacata atgttgattt tccttacgca taatgtcgat taataataaa tgcagtcatt
     1501 tactttaaat gcagtaatgc ttatattata attaaaatca cctgtgatag aattattttc
     1561 tttgatagat tcaatcaatt tttttctaga aacataccta tgtacaaaaa aaggaggagc
     1621 atgcatgtat agctttaaga cattattctg ctacaattgc aagtctgtat tgttgaatct
     1681 tccagaagtt gaaatttcaa agttaaacgg tctgaatttt gtttgtgagt gctgcggaca
     1741 tcgcaacctc ttaacgaaaa accagttctt caaaagcgtt gacacatatg acccctatat
     1801 cagcattcaa agtgtggaca acatcatttc agagagagca ttatattaat taatgaataa
     1861 ttatattttt gtgaccagta catagtatca gcctcttatt attgtaaaat ttaagagctt
     1921 tgaacccctc gtttttaatg ctttagttca aaagtcttaa aaaagaaaaa ggccttaggg
     1981 cctttttctt tttgtgcact catgtgttca tggtaatttc aataaagcag agaaagacgg
     2041 gttttatacc agagttttaa ctatctctcc agtgctttca ctgtcatata tactgtcttt
     2101 cttttgcaaa tcctgctcag ctttgtaaaa gttcttgaat gcaaccgaca gcataagctt
     2161 aatcctgttt aactgattta cttcacttgc ccccggatca taatccaccg caacaatatt
     2221 ggaatcggga tacctccgcc taagctcctt tatcatgccc tttccggtta catgattggg
     2281 caaacaggca aaaggctgca tacacactat gttttgtaca ccgctcttta taagctctat
     2341 catttccgct gtcaaaaacc agccctcacc tgtctgattt ccaacggaca gaatcgcaga
     2401 cgccatttct gccagttcag atattgaaca ggggggacta aatcttttac tctgtgcaag
     2461 tgcttttttc atatcccttc tgtagtattc aataaatgat atcgccaaat tttttattgt
     2521 ccggttaatc tttttcccgg ataaatgggt gtatctgaag ttttcgttat aggcacaata
     2581 caataagaag tcggttaaat ccggcattac cgcctctgct ccctctctct ctattatgtc
     2641 aacaacattg ttgttggcct gagggtggaa tttaacaaga atctctccta ccaaccctac
     2701 cctgggtttt ttgacgttat acacatccag attgtcgaaa tcctccacga ttctgtagat
     2761 gttttctctg aatgttcttt gagagccggt tcttatcgat tccaaacatc tgggtaccca
     2821 agaatcgtac aagagatttg cagaaccgct tattctctca tagggtctca ccctatacaa
     2881 taccctcata agcaaatcac catataccac agccataagg gctttgttta ccagtcccgg
     2941 tctgatttta aacccgggat tcttctccaa acctgcaaaa ttcaaggata atacaggtat
     3001 atgggaaagt cccgcatcct tcagtgcttt tcttataaac ccaatatagt tcgtagccct
     3061 g
//
LOCUS       DAOOJC010000302         3057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_24750_length_3057_cov_3.075283, whole genome shotgun sequence.
ACCESSION   DAOOJC010000302 DAOOJC010000000
VERSION     DAOOJC010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24750_length_3057_cov_3.075283"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="PLA01_08100"
     CDS             complement(<1..1134)
                     /locus_tag="PLA01_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01293.1"
                     /translation="MKRIISLVIFSVIILTTVFSTNVFASSTFERVENTIVILVEFTE
                     TPESLSAYNGTEYTGIIMESDSYYSNLFFDASNNSVYSVRNYFRQQSGGKLDLTPAPE
                     SYGLANDGIIRVKLDFPHPNDYETSGEGYGTLTKDATLNISRAAINKAAQYIDFAYFD
                     KNTNGELTRGELHVNLVLASGSEKKAPGTRSFNADTPLASMGSSPVIIDGVNMMSLPM
                     SFVNSRSYSGDSTLSVYCHEMAHSIGAVDLYTIQSNNIIDESIMHNTDMYPPANLDPF
                     NKMIMGFAEPTIISSSGTYTVNSSLPNNPERYNVLLVPMISEQNTQEYLLIENRQFNG
                     FDTVLSGNHMRGGVAIWHVSLPLGYKLMLSKTRIAFEVSISGAS"
     gene            complement(1383..2357)
                     /locus_tag="PLA01_08105"
     CDS             complement(1383..2357)
                     /locus_tag="PLA01_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD01294.1"
                     /translation="MAAITVENLVKTYTTYKRGSGFLETLKSFVKRNPVTVRAVDDIS
                     FKIEKGVICGILGPNGAGKSTTIKMLCGALFPSGGKIDVMGYYPAKDRIKYVRKIGAV
                     FGQRSQLIWDIPPIDSFAMNKAIYAIKDSDYKKRLDELTELFDVAEVIHKPTRVLSLG
                     ERMKCEFIMAMLHSPDIVFLDEPTIGLDVIAKTKIREVVGKMNQNGTTFILTTHDLED
                     VEQLADHVIIINHGKKVFDDTLPHLKSTLGNKKIVKLTLSKPYIGETINGANIIQRES
                     ELDISLEVDVEKQGINDFLNDLSNFVDFTDISIKELPMDMIITRIYNA"
     gene            complement(2383..>3057)
                     /locus_tag="PLA01_08110"
     CDS             complement(2383..>3057)
                     /locus_tag="PLA01_08110"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HPD01295.1"
                     /translation="NILFPLVTVLIYTNGATFPGWTMWEVLLIQSVYSMSTGAAAMFF
                     YSVVWVTMEHIREGSFETVLLKPVSPLFFIASSNFDPQYGGLFLGGLVLTIVSSAHTN
                     ISGLSGILQFILLFTAGLAVMSGLYLVMAAITFKWVGNARIPEIFDSIITFGKYPAGI
                     FPRGIQVLSTLIVPVSVIGYFPASALLGRLEPMAYISLIPCLLFLAFGIWIYNRMIKL
                     YEGVGG"
BASE COUNT          925 a          638 c          577 g          917 t
ORIGIN      
        1 gcttgctcct gaaatagaga cctcgaaagc aatacgagtt ttcgagagca ttaatttata
       61 accaaggggg agactgacat gccatattgc aactccgcct ctcatgtggt ttccgcttag
      121 tactgtgtcg aatccgttga attgacggtt ttcgattagc aagtattctt gagtgttttg
      181 ctctgaaatc attggaacca gaagaacgtt atatctttca ggattattcg gcaaggacga
      241 gttaacggta taagtacctg aagaagaaat aattgtaggt tctgcaaatc ccattatcat
      301 cttattaaac gggtctaggt ttgctggcgg atacatatcg gtattatgca taatggattc
      361 atcaattata ttgttgcttt gaatggtata taaatcgact gctccaatgc tatgggccat
      421 ttcgtgacaa taaacactaa gagtactatc tccagaataa gatctggaat taacaaatga
      481 cattggcaga ctcatcatat tcacaccgtc tattataaca gggctacttc ccatgctagc
      541 caacggagta tcagcattaa aagatcttgt gccgggagct ttcttctcac tgccgcttgc
      601 aagaactaaa ttaacatgga gttcacctct ggtaagttct ccgttggtat ttttatcaaa
      661 atatgcaaaa tcaatatact gtgctgcttt atttattgct gctctagaaa tgttgagtgt
      721 ggcatctttt gtaagcgttc cataaccctc tccggaggtc tcatagtcgt taggatgggg
      781 aaaatcaagt ttaactctta ttataccgtc atttgccaat ccatagcttt ccggtgcagg
      841 agtcaaatcc aatttccctc cggattgctg tctgaagtag tttctaacag aatatacgct
      901 attatttgat gcgtcaaaga atagattgga ataatatgaa tcgctctcca ttattattcc
      961 agtgtattct gttccgttat aggcacttaa ggattccgga gtttctgtaa attctactaa
     1021 aataacaatg gtgttctcaa ctctttcaaa ggtgctgctg gcaaaaacat tcgtactgaa
     1081 tacagtggta agaattataa cagaaaaaat aaccagcgaa ataattctct tcatacaaaa
     1141 acctcctcaa aataatatct tagaattctc atttaaaatg aaaacttcta tatgtcgaca
     1201 tattaccaaa gttttctata caattttaca acataaagat gagatttaat agattaccaa
     1261 agtaataaca atatttgaga attattactt tggtaatata ctgtttcctt catggccatt
     1321 taatgcaccc gtctgatagg ggaaaggaga cttagataat ttcctttcgg agagacagaa
     1381 atttacgcat tgtaaatcct tgttattatc atgtccatag gcagttcttt tatcgaaata
     1441 tcggtaaaat ccacgaagtt ggacaaatcg tttaaaaagt cattaattcc ctgcttttcc
     1501 acatcaacct ccagtgaaat gtccagttca ctttcccttt ggattatatt tgcaccgttg
     1561 atagtctctc cgatataagg ctttgaaaga gtgagcttta caattttctt gtttcccaat
     1621 gtggatttca aatgcggcag cgtatcgtca aacacctttt tgccgtggtt gataataatg
     1681 acatggtcgg caagctgttc cacatcctca agatcatggg ttgtaaggat aaaagtagtg
     1741 ccgttttggt tcatcttgcc gacaacctca cgtattttag tcttcgcaat tacatcaaga
     1801 ccgatggttg gctcatccaa aaaaactata tcggggctat gcagcatagc catgataaat
     1861 tcgcatttca tcctttcgcc taaagacagg acacgagtcg gtttgtgaat tacctctgca
     1921 acatcaaaaa gctccgtcaa ttcatcaagc cgtttcttat aatcggagtc tttgattgcg
     1981 tatattgcct tgttcattgc aaagctgtcg atgggcggaa tatcccatat gagctgtgat
     2041 ctctgcccga atacggcacc gattttacgg acatacttaa ttcggtcttt tgcaggataa
     2101 tatcccataa cgtcaatttt cccgccggaa gggaagaggg ctccgcatag catctttatt
     2161 gtagtggatt taccggcacc gttgggccct agtataccgc atatgactcc tttttctatt
     2221 ttaaaggaaa tgtcgtccac tgctctaacc gtgaccggat tacgctttac aaagctttta
     2281 agcgtttcta aaaaaccgct tcctctttta tatgtagtat aggtttttac aagattttca
     2341 actgttattg cagccataat aattcatcct ttttcaaaat gattaacctc ccacgccttc
     2401 gtacagcttt atcatcctgt tgtaaatcca tattccgaaa gcaagaaaca acaaacacgg
     2461 aataagggag atataagcca tcggttccag gcgtcccaat agtgccgagg caggaaaata
     2521 tccaatgacc gatacaggaa caataagtgt tgaaagcacc tgaattccac gagggaatat
     2581 acctgccgga tactttccaa aggttattat actgtcgaaa atttcgggga ttcgggcatt
     2641 tccgacccat ttaaaggtaa ttgcagccat aactagatat agcccgctca taactgcaag
     2701 ccccgccgta aacaacagaa taaactggag tatgccgctc aaaccggata tatttgtatg
     2761 tgcggacgaa acaatcgtca aaaccagtcc gccgagaaaa agtccgccat attggggatc
     2821 gaaatttgaa gaggctataa aaaacagcgg cgaaacaggt tttaacaata ccgtttcaaa
     2881 actgccctca cgaatgtgtt ccattgtcac ccatacaaca ctgtaaaaaa acattgcagc
     2941 tgctccggtg gacatggagt aaaccgactg aatcaacagt acttcccaca tagtccagcc
     3001 cgggaatgtc gcaccattgg tataaatgag cacagttacc aacggaaata gaatatt
//
LOCUS       DAOOJC010000303         3049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_24833_length_3049_cov_3.805611, whole genome shotgun sequence.
ACCESSION   DAOOJC010000303 DAOOJC010000000
VERSION     DAOOJC010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24833_length_3049_cov_3.805611"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PLA01_08115"
     CDS             <1..969
                     /locus_tag="PLA01_08115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPD01296.1"
                     /translation="RLINETGKFDIIHAHDWLVAFAARVLKHSYSIPLVATIHATEHG
                     RNWGIHNDTQRYINNVEWWLAFESWRLIVNSEYMKNEVKGIFNIPGDKVNVIPNGVDL
                     NKFNGYHRDIEFRRKFANDNEKIVFFVGRLVNEKGVHVLIDAVPKVSYNYHDVKFVIA
                     GKGPQLDHLKWKANEMGVASKVYFTGYISDEDLLKLYKCVDVAVFPSLYEPFGIVAIE
                     GMLANVPVVVSDTGGLGGIVEHGVDGMKSYTGNANSLADSILEILFNPEKAEQMKKRA
                     LEKVHSIYNWDVITKRTLEVYQGIIDESRRVEWSTPIMKEEHSRYN"
     gene            1730..>3049
                     /locus_tag="PLA01_08120"
     CDS             1730..>3049
                     /locus_tag="PLA01_08120"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPD01297.1"
                     /translation="MAKKKKKLKIIPLGGLGEIGKNITVFEYGDDIVIVDCGIAFPED
                     DMLGIDLVIPDISYLTKNREKVRGIVLTHGHEDHIGALPYVLKDLNVPVYGTKLTLGL
                     LEQKLEEHGILSVVDLRTVQQSDVIELGCFKVEFIHSTHSIADSVALAIFTPVGTVVH
                     TGDFKIDYTPIEGEPLDFARMAELGKKGVLLLMCDSTNVERQGYTMSERTVGDTFDEI
                     FINAKSRILVATFASNVHRIQQVVNASVKFGRKIAICGRSMVNVVNVAMELGYMNVPE
                     GLLIDIDHVHKYPPERLVIITTGSQGEPMSALTRMASGEHKKVEIVPGDLVIISANPI
                     PGNEKLVSKVINDLFKKGAEVIYEALADIHVSGHACQEELKLIHSLIKPKFFLPVHGE
                     YRHLKQHANLANELGMPMENIFIMDIGKVLELTDDSARINGSVTSGRV"
BASE COUNT          956 a          469 c          749 g          875 t
ORIGIN      
        1 cgcctgatta atgaaaccgg gaaatttgac ataattcatg cacatgactg gttggtggct
       61 tttgctgcca gagttttaaa gcattcatac tctatacctc ttgttgctac tattcatgcc
      121 acagaacacg gaagaaattg gggtatacat aatgacaccc agaggtatat aaacaatgtg
      181 gaatggtggt tggcttttga gtcctggagg cttattgtaa acagtgaata tatgaaaaat
      241 gaggttaagg ggatattcaa cattcccggt gacaaggtca atgttattcc aaacggtgtc
      301 gatttgaata agtttaatgg ctatcacagg gatattgaat ttagaagaaa gtttgccaat
      361 gacaacgaaa agattgtgtt ttttgtggga agactggtaa acgaaaaggg agtgcatgta
      421 ttaattgatg ctgttccaaa ggtatcatac aattatcacg atgttaagtt tgtgattgcc
      481 ggtaaaggtc cgcagcttga tcacctgaag tggaaggcca acgaaatggg agtggcaagt
      541 aaggtatact ttacgggata cataagtgac gaggatcttt taaaactata caaatgtgtt
      601 gatgttgctg tttttccaag cctttacgag ccctttggaa ttgttgctat tgaaggaatg
      661 cttgccaatg tgccggttgt tgtatccgac accggaggac tgggaggaat tgtggagcat
      721 ggtgttgacg ggatgaagtc ttataccgga aatgccaact ctctggcaga cagcatactg
      781 gaaatacttt tcaatcccga aaaggctgaa cagatgaaga aaagagcact ggaaaaggtt
      841 catagcatat ataactggga tgtcattacc aaacggacat tagaggtata ccaaggcata
      901 atagacgaga gcagaagggt tgagtggagt actcccatta tgaaagagga gcatagcaga
      961 tacaattgag ataaaaaatt acagggtgcc ggcagattgt ttgccggctt tctttaaaac
     1021 ggcagcttat gatacgtgca tattatgggg ctggaaccca gtgtttttct tcgggtagaa
     1081 ggatgttatc gtgggaggcg tttgtcatat aaaaaagttt tatgtatttg ctaagtaaat
     1141 tattgtttat attattgata attatgctat aataataagg ttgagactaa taattgtatc
     1201 aaatattata taatcaattt atttgataaa taaaaatggt ttagttaagg atggctgaag
     1261 acaagaggga ttttatataa atattaagta atggacaggg tttataacga tatgaatata
     1321 tgaagcgggt tgtgtacaaa tagccgttga cagcaaagaa tattctgaat gaacattaag
     1381 gcttcggaaa tacagaataa aaaacaggat agtatgctgg atatggagtt gtcagaatca
     1441 acctgtctca cggaatgaag tgtttgaata tttgattgac aaacatttca agggcgtttt
     1501 tattccttga acgagatttt gtttgtcaaa acagggctta tcgtttacat acaagctatc
     1561 aatagctcaa actgggattg cttcacaatt atccgtcttt tggagtggta tttcattcca
     1621 taaagcttaa cgggtctgtt aattgatgca agcatgagaa cgggtgctta tgagactatt
     1681 gcgtagttgt taatcaatat aattaataaa agaaagcgga ggttaaattg tggcaaaaaa
     1741 gaagaaaaaa ctaaagataa ttcccttggg cggattggga gagattggta agaacataac
     1801 tgtctttgag tacggggacg acatagtgat tgtagactgc ggaattgctt tcccggagga
     1861 tgatatgctg ggaatagacc ttgtaattcc tgatatatca tacctgacaa agaaccggga
     1921 aaaggttagg ggtatagtcc ttactcacgg acacgaggat catatcggtg cactccctta
     1981 tgttctaaag gatctgaacg ttcctgttta cggtacaaaa cttacccttg ggctattgga
     2041 gcaaaagctt gaagagcatg gcattttgag tgttgttgac cttagaacgg ttcaacaatc
     2101 ggatgttatt gaattgggat gcttcaaggt tgaattcata cattcaactc acagtattgc
     2161 ggattcagta gctctggcga tatttacgcc tgtcgggacg gtagttcaca cgggagattt
     2221 caagattgac tatacaccta ttgaaggaga gcctttagac tttgcacgta tggcggagct
     2281 ggggaaaaaa ggtgttttac tgctgatgtg tgacagcacg aatgttgaga gacaaggcta
     2341 taccatgtct gaaagaacgg taggtgatac ttttgatgag atttttataa atgctaaaag
     2401 cagaatactc gtggcaactt ttgcttcgaa tgttcataga attcagcagg tggtaaatgc
     2461 ttctgtaaaa ttcggcagga aaattgccat ttgcggcaga agcatggtaa atgttgtgaa
     2521 tgtcgccatg gagctgggat atatgaatgt accagaaggt ttgcttattg atattgatca
     2581 tgttcacaag tatccgccgg aaagactggt tataattacg acaggaagtc agggagagcc
     2641 aatgtccgcc ttgacaagaa tggcttcggg cgaacacaaa aaggttgaaa ttgtaccggg
     2701 ggatctggtg ataatttcgg ccaatcccat tcccggaaat gagaaactgg tttcgaaggt
     2761 aataaacgac cttttcaaaa agggagcgga agtaatatat gaagccctgg ctgatataca
     2821 tgtttccgga catgcatgcc aggaagaatt aaaacttatt cacagcctta taaaacccaa
     2881 gttcttttta cctgtacatg gagaataccg tcacttgaag cagcatgcaa atcttgccaa
     2941 tgagttgggc atgccgatgg aaaacatttt tattatggat attgggaagg ttttggagct
     3001 taccgatgac tcagccagaa tcaacggcag tgttacttcg ggaagggtg
//
LOCUS       DAOOJC010000304         3048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_24852_length_3048_cov_2.549282, whole genome shotgun sequence.
ACCESSION   DAOOJC010000304 DAOOJC010000000
VERSION     DAOOJC010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24852_length_3048_cov_2.549282"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    312..321
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            368..1372
                     /locus_tag="PLA01_08125"
     CDS             368..1372
                     /locus_tag="PLA01_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01298.1"
                     /translation="MRRGLEAIKKLGLALIIINILFCSCSNKGSDKEDTSPKQKQLGA
                     EEEGDKIPEQLESIENSIESIIKTLDGPAVETKKHGLQQEPQDKEKQDIVDNNPPNNE
                     TMQNPDEKQNNKSKEEKQNNQNEKQDDEKQSNQDENKEQPSNAKEDKAQEDPWKDIDK
                     IINRMHYQWNSFKPMAFKKNASSSLTDGFSSALNSLTNTVISKNKTNTLLAASYLYSF
                     IPDLYALYKTQTSPEIKRIRYYTRNATLNAMTANWEQADRDIANLKSTWNIYKNLVSA
                     EQQEQSSKLDFSIYELENVIKEKNQPLCDIKGRVVISNIQALEEAMEKGSKKEKSNKE
                     "
     gene            1506..2771
                     /locus_tag="PLA01_08130"
     CDS             1506..2771
                     /locus_tag="PLA01_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HPD01299.1"
                     /translation="MKVTGLIVEYNPFHNGHLYHLEESKKICGADYTVCVMSGNFIQR
                     GEPAVVNKWARANMALSSGADLIIELPFPYAMSSAEFFASGSVKILNDIGIVDFLCFG
                     SEHGNIDELDFIARILIDEPHAYKSFLKQELDRGISYPAARDTAIKEYIKHSNTARQV
                     HAESLLSSSNNILGIEYLKALKRFNSKIIPFTIKRVNNEYGTKQITGNISSATSIRSF
                     ISTNSSQALKDVLQKVLPEASLNILLEEMAEGRGPVFAEDFYTIIASQLRRMKPGQIK
                     GYPYVSEGLENRIKASAGSSGTYDELVEGICTKRYTRTRIQRILMSILTGMTLEQADM
                     FNRYNGPQYARVLGFNKKGQQLLSLIKQKSVIPLVMKTADFVKSCNPLLKSMLELEAM
                     STDIYVLGYKNPAYKKAGQEYTQNVVIKA"
     gene            complement(2785..>3048)
                     /locus_tag="PLA01_08135"
     CDS             complement(2785..>3048)
                     /locus_tag="PLA01_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPD01300.1"
                     /translation="IVITIEGDTGNVVAGPDIISRGFVYVRESEDLMDEIREVCKSAL
                     QKCEDKKKNDWSTKKSIIRDALRDYLFEKTKRRPMILPIIMEV"
BASE COUNT         1073 a          597 c          574 g          794 t
ORIGIN      
        1 taaacataaa tccactgatc cacatcattg tccatattca gctccttctg atagaacctc
       61 tgcagaaaat tcttaatgtc tcccggtttt tgtgacaata aatttacagt aacatacatg
      121 taatgaattc ctcctacttt tttgttttta aaaaagaaag tatatatcat tttgaaaatt
      181 caacgaaaca acaaagaaac ctttatgtaa ttttttcttt cgagatatca tcgacatagc
      241 ggtattataa ataattattt ttgaaataat ataaacatag gagactcgat ttatgaaaat
      301 actgcgaatt tnnnnnnnnn naagtggagg gaatcagcag caaatattgc ttgactgctg
      361 gaaaattatg agaagaggat tggaggcaat aaaaaaactc ggcttggctt taataataat
      421 aaatatttta ttttgtagct gcagcaataa aggttcggac aaggaggaca cgagcccaaa
      481 gcaaaagcag ctcggagctg aagaggaagg agataaaatt cccgaacaat tggaaagcat
      541 tgaaaacagc attgagtcta taattaagac actggacggt cctgccgttg aaacaaaaaa
      601 gcacggttta cagcaagaac cccaggataa agaaaagcaa gacatagtgg ataataatcc
      661 tccaaacaat gaaacaatgc aaaatcccga tgaaaagcaa aacaacaaaa gcaaagagga
      721 aaaacaaaac aatcagaatg aaaagcaaga cgacgaaaag caaagtaatc aagatgaaaa
      781 taaagaacag ccttctaatg ccaaagagga taaagcacag gaagatccat ggaaggatat
      841 tgacaaaatt ataaacagga tgcattatca atggaacagt tttaaaccca tggcttttaa
      901 aaagaacgca agcagcagct taacggacgg cttcagttca gcactgaaca gccttacaaa
      961 cacagttata agcaaaaata aaacgaatac tttattggct gccagttatt tatattcctt
     1021 tatacctgac ctttatgcac tgtacaaaac tcagacttcc cctgagatta aaaggatccg
     1081 gtactatacc cgtaacgcaa ccctcaacgc aatgactgcc aattgggaac aggccgatag
     1141 ggatattgca aacttgaaat ctacctggaa tatatataaa aatctggttt cagcagaaca
     1201 acaggaacaa tcaagcaagc tggatttctc tatatacgag ctcgaaaacg ttataaaaga
     1261 aaagaaccaa cccctgtgcg atattaaggg acgggtggta atttccaata ttcaggctct
     1321 cgaagaagcc atggaaaagg gttcgaaaaa ggagaaatca aataaagaat agaaatttga
     1381 tatgttaaaa agtactggta taatggtata taatatatta ttatattata tgactacttg
     1441 gggctctaaa caggcttaca attgtctttt tgtctgtcag gagcccatag aagggaaaac
     1501 atcctatgaa agttacgggc ttgattgttg aatacaatcc ttttcacaac ggccaccttt
     1561 accatcttga agaatcaaaa aaaatatgcg gtgccgacta taccgtatgc gttatgagcg
     1621 gaaactttat ccagcgtggc gaaccggctg ttgtaaacaa gtgggcacgg gcaaatatgg
     1681 ctctgtcttc cggtgctgat ttaataattg agcttccctt tccctatgcc atgtccagtg
     1741 cagagttctt cgcctccgga tcggttaaaa tattaaatga cattggtatc gtggattttt
     1801 tatgttttgg aagtgaacac ggaaatatcg atgagcttga ctttattgcc cggattctta
     1861 ttgatgaacc ccatgcgtat aaaagctttt taaaacaaga actggacagg ggaatatcct
     1921 atcctgcggc ccgtgatacg gcaataaaag aatacatcaa acactcaaac acagcaagac
     1981 aagtccatgc agaaagcttg ctttcttctt caaacaacat ccttggcatt gaatacctaa
     2041 aagctctaaa gaggtttaac agcaaaataa ttccctttac gataaagagg gttaacaacg
     2101 aatacggcac aaaacagata accggcaaca tatccagtgc cacgtcaata agaagcttta
     2161 tttccacgaa ctcctctcaa gcattaaagg atgttcttca aaaggtgctt cccgaagcaa
     2221 gccttaatat actgcttgag gaaatggctg aaggacgggg accggttttt gccgaggatt
     2281 tttataccat tattgcatca caattaagaa ggatgaagcc cggacaaatc aaaggctatc
     2341 cctacgtatc cgaaggtctt gaaaacagga ttaaagcctc agccggctca tccggcacat
     2401 atgatgaact ggtagagggt atctgtacaa aaaggtatac aagaaccaga atacaaagga
     2461 tacttatgag catattgaca ggaatgacct tggagcaagc cgacatgttc aaccgctata
     2521 acggtcctca atacgcccgg gtattgggct ttaacaaaaa aggccaacaa ctgctctctt
     2581 tgattaagca aaagtcagtg attcccttgg tgatgaaaac cgcagacttt gttaaatcct
     2641 gcaatcccct attaaaaagc atgcttgaat tggaagcaat gtcaacggat atttatgtgc
     2701 tgggatataa aaatccggcc tataaaaagg ccggacagga atatacacaa aatgttgtaa
     2761 taaaggctta agagactttt cagattatac ctccattatg ataggtaaaa tcatcggtct
     2821 tctctttgtc ttctcaaaca aataatccct cagtgcatcc cttatgatgc tcttttttgt
     2881 cgaccagtca ttctttttct tgtcttcaca tttttgcagt gcacttttgc acacttccct
     2941 tatttcgtcc ataagatcct ccgactcccg gacataaaca aaacctcttg atatgatatc
     3001 gggaccggca acaacatttc ccgtgtcacc ttctattgta ataactat
//
LOCUS       DAOOJC010000305         3046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_24872_length_3046_cov_3.494483, whole genome shotgun sequence.
ACCESSION   DAOOJC010000305 DAOOJC010000000
VERSION     DAOOJC010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24872_length_3046_cov_3.494483"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PLA01_08140"
     CDS             <1..241
                     /locus_tag="PLA01_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPD01301.1"
                     /translation="LVIIAQRPCALLKNVKHEGPHRINTQKCKKCKMCMRLGCPAIVD
                     MGDRIEINEALCVGCGLCSKVCNFDAIEKAGEINE"
     gene            234..809
                     /locus_tag="PLA01_08145"
     CDS             234..809
                     /locus_tag="PLA01_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPD01302.1"
                     /translation="MNKHNILIVGVGGQGTLLASRVLGNVALKRNYDVKVSEVHGMSQ
                     RGGSVVTYVKMGDRVYSPIIEKGEADIIIAFEQLEALRWADYLKEDGKLLVNEQKISP
                     MPVIIGKEKYPENIMEELKKSYKNVISLDAFKIAKDCGNMKAVNTVLLGLLAKSINID
                     KEVWIEALKETVPQKVLDVNLKAFEEGYKKG"
     gene            851..2152
                     /locus_tag="PLA01_08150"
     CDS             851..2152
                     /locus_tag="PLA01_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPD01303.1"
                     /translation="MQYWNPTYECMHREEMTRVQTARLIDTVKKVYHNVPFYREKMQK
                     KGIEPGDINSVEDLKKLPFTYKQDLRDTYPYGLFAAPLSEIVRIHASSGTTGKQTVVG
                     YTRKDIDTWAEVAARTLTSAGADRNSFIQVAYGYGLFTGGLGIHYGAERVGASVIPIS
                     GGNTKRQIQIMKDFGTTLLACTPSYALYMAEEMEEMGIKKEELQLKSGVFGAEPWSEN
                     MRKEIEERLGILAIDIYGLSEVIGPGVATECSCRCGLHIQEDHFIPEIIDPVTEEVLP
                     YGSKGELVFTTVTKEGLPLIRYRTRDISTLNYEKCECGRTTVRMSKVSGRSDDMLIIR
                     GVNVFPSQVESVLLEFGETAPHYLMIVDRVDNLDVLEIWVEVNQDMFSDQVKRIEDVE
                     KKIRKAIEITLGINAKVKLVEPKTIERSEGKAKRVIDKRKI"
     gene            2206..2637
                     /locus_tag="PLA01_08155"
     CDS             2206..2637
                     /locus_tag="PLA01_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPD01304.1"
                     /translation="MLVKQISVFLENKSGRLDEVTKVLADNNINISALSIADTTDFGI
                     LRIIVNDPDKAEKVLKENDFAVSSTEVIAIAVPDQPGGLEKALKALNEASIGIEYMYA
                     FVGKTEEEALVILRVEALEEAADKLVKNGVKVLSADKVYKL"
     gene            2652..>3046
                     /locus_tag="PLA01_08160"
     CDS             2652..>3046
                     /locus_tag="PLA01_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD01305.1"
                     /translation="MELKKEPLAYRMMPKTLEQFFGQEEIVGEGKLLSRMIKADRISS
                     IILFGPPGTGKTSLARIIANSTKANFEKLNAVTSGVADIKRIVSDTQNRILNPEGRTV
                     LFVDEIHRFNKAQQDALLPYVEDGTIILVG"
BASE COUNT          989 a          477 c          785 g          795 t
ORIGIN      
        1 gttggtaatc atagcacaaa ggccttgtgc acttttgaag aacgtgaagc atgaaggacc
       61 ccatagaatt aatacccaga agtgtaaaaa gtgcaaaatg tgcatgagac tgggatgccc
      121 tgccattgtc gacatgggag accgcataga aataaatgaa gctttgtgcg taggctgcgg
      181 attatgttca aaggtatgta actttgatgc gattgaaaag gctggtgaaa taaatgaata
      241 aacacaatat attgatagtg ggagtaggcg gacagggtac attgcttgca agcagggttc
      301 ttggaaacgt tgctcttaaa agaaattatg acgttaaggt ttcggaagtc catggtatgt
      361 cacagcgtgg cggaagcgtt gtaacatatg taaaaatggg tgatagggta tattctccga
      421 taattgaaaa gggagaggcc gatataatca tagcatttga acagttggag gctctaaggt
      481 gggcagatta cttgaaagaa gacggcaagc ttctggttaa tgagcagaaa ataagtccga
      541 tgccggttat tatcggaaaa gaaaagtatc cggaaaacat aatggaagag ttgaagaaaa
      601 gctataaaaa cgttatttct ctggatgcgt ttaagatagc caaggattgt ggaaacatga
      661 aggctgttaa tacagtgttg ctcgggcttt tagcaaagtc tataaatatt gacaaggaag
      721 tatggattga ggcattaaag gaaaccgttc cgcaaaaggt acttgacgta aaccttaaag
      781 cctttgaaga aggttataaa aagggataga tccaagatct taagaatata aaatgtatgg
      841 gggtatattt atgcagtact ggaatcctac gtatgaatgt atgcacaggg aagagatgac
      901 aagagtacaa actgcaaggc ttattgatac tgtaaagaag gtttaccata acgtaccttt
      961 ttacagagaa aagatgcaga agaagggtat tgaaccgggg gatataaatt cggtggagga
     1021 tctcaaaaag ctcccgttta cctataaaca ggatttaagg gatacttacc cctatggact
     1081 gtttgctgca cctttgagtg aaattgtaag gatacatgct tcgtccggta ccaccggaaa
     1141 gcaaacggta gtaggttata ccagaaaaga tatcgatact tgggctgaag tagctgcaag
     1201 aacattaact tcggctggag ccgacaggaa ttcatttatc caggtggctt acggttacgg
     1261 cctttttacc ggcggtctgg gaatacacta tggtgctgag agggtaggtg catcggttat
     1321 acctatctcc ggcggcaata caaaaagaca gatccagata atgaaggatt tcggaaccac
     1381 tcttttagcc tgtaccccgt cgtatgcctt gtatatggcc gaggagatgg aggagatggg
     1441 cattaagaag gaagaactac agcttaaatc cggtgttttt ggtgccgaac cgtggtctga
     1501 aaatatgagg aaagagatcg aggaaagatt gggaatactc gctattgata tttacggatt
     1561 aagtgaggta attggtcccg gtgttgccac tgagtgcagc tgcagatgcg ggcttcacat
     1621 acaggaggat cattttattc cggagattat cgatcctgtg actgaagaag tgcttcctta
     1681 cggttcgaaa ggtgagcttg tgttcacaac agttaccaaa gaaggtttgc cgcttatcag
     1741 gtatagaaca agagatattt ccaccttgaa ttatgaaaaa tgtgaatgcg gaaggactac
     1801 tgtcaggatg agcaaggtat cgggaaggtc cgatgacatg cttattataa gaggagtcaa
     1861 tgtattcccg tctcaggttg aaagtgttct tcttgagttt ggcgaaacag ctccccatta
     1921 tctcatgata gttgacaggg tagataatct ggacgttctg gaaatatggg tagaagtgaa
     1981 tcaggatatg ttctctgacc aggtcaagag aattgaggat gttgaaaaga aaataagaaa
     2041 ggctatagaa attactctag gaattaatgc caaggttaag cttgttgagc ctaagacgat
     2101 agaaagaagt gaaggaaagg caaagcgtgt gatagataag cgtaagattt aacaaaacca
     2161 gatatcaaat gtatggttat tccataaaaa tgggaggggt aaaacatgct tgtaaaacag
     2221 atttccgttt ttttagagaa caaatcaggc aggctcgacg aggttactaa ggttcttgca
     2281 gacaacaaca ttaacattag tgctctctcc atagctgaca ccactgattt cggaattttg
     2341 aggattattg tcaatgatcc cgataaggct gaaaaagttt taaaggaaaa tgattttgca
     2401 gtaagctcta cagaagtaat tgctatagca gttcccgacc agccgggcgg gcttgaaaag
     2461 gcattgaagg ctttgaatga ggcatccata ggtattgaat acatgtatgc ttttgtagga
     2521 aaaacagagg aagaggcatt ggtaatatta agggttgaag cactggaaga agctgcggat
     2581 aaacttgtaa agaacggtgt taaagtatta tcagcagaca aggtatataa gctgtagtta
     2641 ggaaggatgt tatggaatta aaaaaggagc ctctggctta caggatgatg ccgaagacat
     2701 tggaacagtt ttttgggcag gaggagatag taggtgaagg taagctttta agcaggatga
     2761 taaaagcaga cagaatttca tccattatac tttttggacc tcccggaacc ggtaagacat
     2821 cccttgcaag gattattgca aacagtacta aagcgaattt tgaaaagctc aatgctgtta
     2881 cttccggtgt tgccgatatc aagagaattg tctccgatac ccagaaccgg atacttaatc
     2941 ctgagggcag aactgtgtta tttgttgatg aaattcacag gtttaacaag gctcagcagg
     3001 atgcactgct tccttatgtc gaagacggta caataattct tgtggg
//
LOCUS       DAOOJC010000306         3031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_25042_length_3031_cov_3.111895, whole genome shotgun sequence.
ACCESSION   DAOOJC010000306 DAOOJC010000000
VERSION     DAOOJC010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25042_length_3031_cov_3.111895"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PLA01_08165"
     CDS             complement(<1..541)
                     /locus_tag="PLA01_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01306.1"
                     /translation="MNFEILVRAALKLKNLALLAMILGIFIVTGFSWPFLFIGAAGYV
                     YFIMQTMKDEKFLKEFNDEQQIEGIHNLNEKCNALYQSLLRKLPPGMRKRIRNIYNEK
                     ETMISYFSQDDSNPLRQKIAEQAINLVMAYFRLIYHFTQRSQQLSSININKVMDRINN
                     NKKKMEFLSNSVAVLNVQKA"
     gene            complement(602..2239)
                     /locus_tag="PLA01_08170"
     CDS             complement(602..2239)
                     /locus_tag="PLA01_08170"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HPD01307.1"
                     /translation="MGERRIKWGNVIIFLLIIAAGIAAYCSRNQFSFGGNKDKPVAFD
                     NSGKDGKKVDDEFDFDNEFQIWDDDIVPPSGDEFIMGLDSWIGGTPVLIGLNREYNRD
                     YRLNLRVEYIHADADRIKALAEGKIHATEMSLPSFMRFQEQYPGAGIIVGITDFSRGA
                     DGIVAKTEVKDLNDIEDRRVSYVKNGTGKFILNKFLRLVGLRYQDIKPIERESMSEVI
                     EDLRRGTADVIVSWSPDMNIAINEINAEKPNSVKMLITTKEVPNLIPTMLIVNKGYAE
                     KNADKVESFIKTWYASSKHILERPEKAYGTLAELMNESQEYGEVSKVDVEESFRDVKL
                     MSLDDNLSYFGLSTADNKVESIINDTIETWKRYGDLDFDFEISGKIYKESFLAEIDGD
                     EELLVGVLDTETTGSVGEEKEFEKQDGKSIEENTEKVAKVDIPPVYYDSGKATVKAES
                     LPVLDEVLEILSQFPEYYLIVDAHTDSVGSNESNKLLSQNRAAEVKKYLVQHGVNENR
                     IVARGWGEERLLIPNDVSEEDKAKNRRTEFILTREFD"
     gene            2476..2940
                     /locus_tag="PLA01_08175"
     CDS             2476..2940
                     /locus_tag="PLA01_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01308.1"
                     /translation="MSDGSGFGIGPQTGGSFKSLLSKYIGETVTIFTKSGGQSGAGFT
                     GVVLSVNDCFVRLITRIGPPPACSLGNACTDFNVGYGYDKGNPKVAGYSDCGHSCGFK
                     KGYDAYPYSPVQTADKLGAYNAGGWDNYPVYTVGSVADIPISAIVSFVHNAV"
BASE COUNT          867 a          687 c          454 g         1023 t
ORIGIN      
        1 cagccttttg aacatttaat acagcaacag aattactaag aaactccatt tttttcttgt
       61 tattgttgat gcggtccatt actttattaa tgtttatgga acttaactgc tgacttctct
      121 gggtaaagtg ataaataagc ctaaaatacg ccataacaag gttaattgcc tgctcggcaa
      181 tcttttgtct taacggatta ctgtcatcct gggaaaaata agatatcatc gtttcctttt
      241 cattgtaaat attccttatc ctttttctca tgcctggggg taactttctc aacaagcttt
      301 gataaagggc gttgcatttt tcgttaagat tatgtatgcc ttctatctgc tgttcgtcat
      361 taaactcctt taaaaacttc tcatccttca tggtttgcat aataaaatat acatagccag
      421 ccgctcctat aaaaagaaac ggccaactaa atcctgtaac aatgaatatc cccagtatca
      481 tggcaagtaa tgctaagttc tttagtttta aagcagctct cactaaaatt tcaaaattca
      541 ttttttaact ttttacctcc aaaaccattg gtataaaaat gttggattaa atatcatact
      601 attaatcaaa ttcccttgtc aaaataaact cagtccttct gttttttgcc ttatcctctt
      661 cagaaacatc atttggaatc aggagcctct cttctcccca accccgggct actattctgt
      721 tctcattaac tccatgctgt acaagatatt tttttacttc cgcagccctg ttctgtgaca
      781 ataatttgtt ggattcatta cttcccacag aatctgtatg agcatcaaca ataagataat
      841 attccggaaa ctgcgacaat atttccaata cttcatctaa aacaggcaag gattctgcct
      901 ttaccgttgc ttttcccgaa tcatagtata ccggcggaat atccactttt gccacctttt
      961 ccgtattctc ttcgatgctt tttccatcct gtttctcaaa ctctttctct tcaccaacac
     1021 tacccgtagt ttcagtgtcc agtactccca caagaagttc ctcatcaccg tctatttctg
     1081 ctaaaaagct ttccttatat attttccctg aaatttcaaa atcaaaatcc aggtcgccgt
     1141 atctcttcca tgtctctatc gtatcgttaa ttattgactc taccttgtta tcagcagtac
     1201 ttaaaccaaa atagcttaaa ttgtcatcta atgacatgag cttaacatct ctaaaggact
     1261 cctcgacatc caccttacta acctcaccat attcctgaga ttcattcatt aactctgcaa
     1321 gcgttccata agccttttcg ggtctttcca aaatatgctt ggaagaagca taccaggttt
     1381 ttataaagct ctcgacttta tctgcattct tctctgcata acccttatta actatcaaca
     1441 tggtaggaat cagattaggt acctctttag tagtaatgag catcttcact gaattcggtt
     1501 tttcagcgtt tatttcgttt atggcaatgt tcatatccgg actccacgat actattacat
     1561 ccgcagtacc tcttctcaaa tcctctataa cttccgacat gctttcccgt tcaattggtt
     1621 ttatatcctg gtaccttaaa cctaccagtc ttaaaaactt gtttagtata aatttgcctg
     1681 tgccgttttt aacataagac acccttctgt cttcgatgtc attgaggtcc tttacctcgg
     1741 tttttgcaac aattccatca gcaccacggg aaaaatccgt aatacccact ataataccgg
     1801 caccgggata ttgttcctgg aatctcataa aggatggaag cgacatctct gtagcgtgta
     1861 tttttccttc tgccaatgcc tttattctgt cagcatccgc atgtatatat tctactctca
     1921 ggttcaatct gtaatccctg ttatattctc tgttcaaacc aattaaaaca ggagttcccc
     1981 caatccaact gtccaatccc ataataaact catcacccga aggaggaaca atatcgtcat
     2041 cccaaatttg aaactcatta tcaaaatcaa attcatcatc gacctttttt ccatcttttc
     2101 cgctattgtc gaaagcaaca ggtttatctt tattgccccc aaaactaaat tgattcctgg
     2161 aacaataagc tgcaattccg gcggctatta taagtaaaaa tataattaca tttccccact
     2221 ttattcttct ttctcccatc cctttgcctc ctcctgcaat aaaattagct ataaaaaaat
     2281 tatatcattt tatttcgata ctgcaacata tattcttgtt tgttcctgtt tgccctgttt
     2341 aagcgttctt ctgcaaatta ttcaacttca tctaattcac ccttaatcaa ccgattaaat
     2401 gtaaccatta tgcagttatt aacataatat gtattacaga tattatttgt ttataaacga
     2461 aaggtggaat tataaatgtc tgacggaagt ggctttggaa ttggtcctca aacaggtgga
     2521 agttttaagt ctttactttc aaagtatatt ggagaaacag ttacaatatt tacaaaaagc
     2581 ggtgggcaat ccggtgccgg ttttactggc gtagtcctct cggtaaacga ttgctttgta
     2641 cgtcttatta caagaatcgg accaccaccg gcatgctccc tcggcaatgc atgtacagat
     2701 tttaatgtag gatacggata tgataaaggt aatccaaaag tagcaggtta ctccgattgt
     2761 ggtcacagct gcggttttaa aaaggggtat gatgcatatc cttattctcc tgtacaaact
     2821 gctgacaaat taggagcata taatgcaggt ggatgggata actatccggt ttataccgta
     2881 ggttcggtag cagatatacc tatttcagca atcgtttctt tcgtccacaa tgctgtataa
     2941 acaacatata caacaaaagc cggggacaat ttatgcccgt gttcataggg acacatactg
     3001 aaccgtgggt tttgtaaagg caggcagcag a
//
LOCUS       DAOOJC010000307         3007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_25281_length_3007_cov_4.606030, whole genome shotgun sequence.
ACCESSION   DAOOJC010000307 DAOOJC010000000
VERSION     DAOOJC010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25281_length_3007_cov_4.606030"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PLA01_08180"
     CDS             <1..750
                     /locus_tag="PLA01_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPD01309.1"
                     /translation="YEMGRMFRNEGMSVKHNPEFTLMEVYEAYNDYKGMMELTENLFS
                     YVAKEVLGTTVITYQSQEIDLTPPWNRMTMTEAVLKYTGIDFSKIESDEEARRIANEK
                     QVHVEETLSKGEVLNLMFEEFVEEHLIQPTFIMDYPVEVSPLTKRKPDEPSLTERFEL
                     FITGREMANAYSELNDPIDQRERFINQVKKREAGDEEANMMDEDFVLSLEYGMPPTGG
                     LGVGIDRLVMLLTDSYSIRDVLLFPTMKPRD"
     gene            812..1126
                     /locus_tag="PLA01_08185"
     CDS             812..1126
                     /locus_tag="PLA01_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD01310.1"
                     /translation="MSDDLRFRQEDLGDSVKLSLSGEVDIYTSQELKENLYNIVDANK
                     TDLIIDCRELNYIDSTGLGIFVGALKKAKEHDKKMVITGLKDNIKKLFIITGLDKVFT
                     IE"
     gene            1138..1530
                     /locus_tag="PLA01_08190"
     CDS             1138..1530
                     /locus_tag="PLA01_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003521553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD01311.1"
                     /translation="MGTECDRIEITLPFKADYVSLARLAVSGICSRMGFDIETIEDIK
                     VAISEVCSKIVSVGSETAHGYTIVYDVAESVLNVKYFCDDPSIKCIFSAERDGLAIHI
                     INALMDRVELCNEANYIVSMSKTLEVVK"
     gene            1533..2288
                     /locus_tag="PLA01_08195"
     CDS             1533..2288
                     /locus_tag="PLA01_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010680915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HPD01312.1"
                     /translation="MTWDIESTNNNADHNEGLFEKYKETESVEIRNEIVSRYLYLADI
                     ISKKFLNRGIDYEDIYQVASIALIKAVERFEPQKGVKFISFATPTIIGEIKRFFRDKA
                     SVIRIPRRIYELYQKVNNAREHLTQELQRPPRVDEIAGYLDITEEKVLEIIESNNVHN
                     IQSFDKNAYTDDDVMLYETVGQDDKTFEEIENKDFLKKSIDNFNDAEKEFIQMRYFGN
                     MTQKDIARKFGVSQMYVSRLEKKVLERFRRMMS"
     gene            2435..2878
                     /locus_tag="PLA01_08200"
     CDS             2435..2878
                     /locus_tag="PLA01_08200"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD01313.1"
                     /translation="MKLKRTCEIKVPGEKSDIRRLVNDIVKFLCDCNGCINDVILFDI
                     KVVLNELIINAVTHGNKGNIDKCVKVKIGITENDYAFFVIEDEGEGFDFKSFIEKDFE
                     IEDTVDFMQLKESGRGVLIVKRLCDKLKFNDKGNKAVVLKKLNKD"
BASE COUNT         1034 a          373 c          719 g          881 t
ORIGIN      
        1 tacgaaatgg gaagaatgtt tagaaatgag ggaatgtcgg tcaaacataa ccccgaattc
       61 acactcatgg aggtttatga ggcatataac gattacaagg gaatgatgga gctcactgaa
      121 aacctgttct catatgttgc aaaggaggtg cttgggacta cggtaataac ctaccagagt
      181 caggagattg atcttactcc gccatggaac agaatgacta tgactgaggc tgtgctgaaa
      241 tataccggta ttgatttttc aaaaattgaa agcgatgaag aggcaagaag gattgctaat
      301 gaaaaacagg ttcatgttga ggaaacgctt tcaaagggtg aggtgttgaa ccttatgttt
      361 gaggagtttg ttgaggagca cctgattcaa ccaaccttca taatggatta tccggttgag
      421 gtttcacctc ttacaaaaag gaagcctgac gagccttctt tgacagaaag gtttgagttg
      481 tttataacgg gaagggagat ggctaatgct tattctgagc tgaacgaccc tattgatcaa
      541 agggaaaggt ttatcaatca ggttaagaag agagaagccg gcgatgaaga agcaaatatg
      601 atggatgaag actttgttct ttctctggaa tacggtatgc ctccgacagg cggattgggg
      661 gtagggattg acaggcttgt catgctgctt accgattcat attcaataag ggatgtcttg
      721 ttatttccta caatgaagcc aagggattaa ctgtaaaccg gtatataaaa atgcattgtt
      781 taatgttatt aaagaaaaag gaggtttaag tatgtccgat gatttgaggt ttaggcaaga
      841 ggatcttggc gattccgtta aattgagtct ctcgggagaa gtggatattt atacatctca
      901 ggaactcaaa gagaatctat ataatattgt agatgccaat aagaccgatt tgataattga
      961 ctgcagggaa ttgaactata ttgacagtac ggggctgggg atttttgtag gggctttaaa
     1021 gaaagccaag gaacatgata aaaagatggt tataacaggc ttgaaggaca acattaaaaa
     1081 gctttttatt ataaccgggc tggataaagt atttacgatt gagtagaggt gataagagtg
     1141 ggaaccgaat gtgacagaat tgaaattact ctgccgttta aagctgatta tgtcagtctt
     1201 gccagactcg ctgtttccgg aatttgcagc agaatgggat ttgatatcga gaccatagag
     1261 gatataaagg ttgcaatatc tgaggtttgc agtaagatag tcagcgtcgg aagcgagact
     1321 gcacacggtt atacaattgt atatgacgtt gcggaaagtg ttttaaacgt taaatatttt
     1381 tgtgatgacc cttctataaa gtgcatattt agtgctgaga gggacggact ggcgatacat
     1441 ataataaatg cattgatgga tcgggtggag ttgtgcaatg aggcgaatta tatagtttct
     1501 atgtcaaaaa ctcttgaggt ggttaagtag ttatgacatg ggatattgaa agcacaaata
     1561 ataatgccga ccacaacgaa gggttattcg aaaaatataa agagactgaa agtgttgaaa
     1621 taaggaatga aattgtaagc agataccttt atcttgcaga cattatttca aaaaagtttc
     1681 tgaaccgcgg tattgattat gaagacattt atcaggttgc gtctattgct ttgattaagg
     1741 ctgtagagag gtttgagcct caaaagggtg taaagtttat aagctttgca accccaacaa
     1801 ttattggcga aataaaaagg ttttttaggg acaaggcatc ggtaataaga attccaagaa
     1861 gaatttatga gctttatcag aaggttaata atgcaaggga acatctgaca caggaattgc
     1921 aaagacctcc gcgtgtagac gagatagcag gatatttgga cattactgaa gaaaaagtat
     1981 tggagataat cgaatcaaat aatgttcaca atattcagtc ttttgataaa aatgcatata
     2041 ccgacgatga tgtaatgctt tacgaaaccg ttggacagga tgacaaaacc tttgaagaaa
     2101 tagaaaacaa ggatttttta aaaaagagca ttgataattt caatgatgct gaaaaagagt
     2161 ttatacaaat gagatacttt ggcaacatga cacagaaaga cattgccagg aagtttggag
     2221 tgtctcaaat gtatgtttca aggcttgaaa aaaaggtgct ggaacggttt agaagaatga
     2281 tgtcttgaac tctcaatttg tattctgcat atggcgtttt tgttttttta ataatccgta
     2341 gatatatacg gaaggtgaat aatgataatg tttacaccaa tactataatg ggtaattatt
     2401 attgagagta aatattatta aggagaaggg taagttgaag ttaaaaagaa cttgtgagat
     2461 aaaggtaccg ggcgaaaaaa gtgatattcg aagactggtt aacgatatag tcaagttttt
     2521 gtgtgattgc aatgggtgta ttaatgatgt cattttattt gatatcaagg ttgttctaaa
     2581 tgaattgata ataaatgcag ttactcacgg taacaagggt aacattgata agtgtgtaaa
     2641 agtgaaaatc ggaataacag aaaacgatta tgcatttttt gttattgagg acgagggaga
     2701 agggtttgat tttaagagct ttattgagaa ggatttcgaa attgaagata cggttgattt
     2761 catgcaattg aaagaaagcg gaagaggagt actgattgta aaaaggctct gtgataaatt
     2821 aaaattcaat gataagggaa ataaggcagt tgtgttgaag aaactgaata aggattagtt
     2881 attataccgt attaattaat aatttcgact attctgtaga ataattgagg attttattaa
     2941 taaattaatt taattatgat taatcgtata gtcgaaggtt tgaatcaatg tgaagtagat
     3001 gatatat
//
LOCUS       DAOOJC010000308         3004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_25330_length_3004_cov_3.253645, whole genome shotgun sequence.
ACCESSION   DAOOJC010000308 DAOOJC010000000
VERSION     DAOOJC010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25330_length_3004_cov_3.253645"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..1521)
                     /locus_tag="PLA01_08205"
     CDS             complement(232..1521)
                     /locus_tag="PLA01_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HPD01314.1"
                     /translation="MKKTISFILCLLFIYAFFVNPAPVKAADFAKGADVSWLLQMEEQ
                     GYKFYDDNGVAKDCLQILKEHGINSIRLRVWVNPSDDPYSGHCSKEDTVKIAKRCADM
                     GFRIMIDFHYSDSWADPGKQYKPAAWNNHDINRLVTDVYNHTYDVLSALKSNEVKPEW
                     VQVGNETNDGMLWEEGKASKNMKNFALLVSSGYDAVKAVFSDAKVIVHISNGHNNSFF
                     RWIFDGLRDNGAKFDVIGMSLYPEVDNWSSLSNQCLSNMNDMVSRYGKEVMICEVGMP
                     TYPASTSKAFLTDIINKTRTVSGGKGLGVFYWEPQCYNWAGYGKGAWYDDGRPSEALD
                     AFLEGVIPTPTPTSKLTPTPTFTLGLPGDLNGDGEINSTDLTLLKRYILHKIDDFPVA
                     DDKKAADLNEDGYIDSMDITILKRYLLRKIKTLPYVV"
     gene            complement(1682..>3004)
                     /locus_tag="PLA01_08210"
     CDS             complement(1682..>3004)
                     /locus_tag="PLA01_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HPD01315.1"
                     /translation="MVQQENLDIRTITMGISLRDCACSNGKESRRKIYDKITRLAQNL
                     VKVGEDIEKEYGVPIVNKRISVTPVSIIAESCDDENYVEFAKTLDRAADTVGVNFIGG
                     FSALVHKGYTVGDKKLIASIPEALSSTKKVCSSVNLATTKSGINMDAVKEMGRIIKKS
                     AELTADSGGMACAKLVVFANVPEDNPFMAGAFHGVGEPECVINVGVSGPGTVKAALEK
                     VKGADFETVSETIKKTAFKITRMGQLVAYEASRRLGVAFGIVDLSLAPTPAVGDSVAH
                     ILEEIGLERCGTHGTTAALALLNDAVKKGGTMASSYVGGLSGAFIPVSEDAGMIDAVK
                     AGALSIEKLEAMTCVCSVGLDMIVVPGDTSAETISAIIADEAAIGVINNKTTAVRIIP
                     APGKKVGDVVEFGGLLGSGPVMKVSKFSSKEFIERGGRIPAPIHSLRN"
BASE COUNT          844 a          761 c          489 g          910 t
ORIGIN      
        1 caattcttac atttctttac attctaaatt attaagtcat tttgcaaatt cctatgaatt
       61 ttttttgcat aaattgcaaa aacaagaaag ctacaatgta ttatattaca ttgatttcta
      121 tttttcaaga aaatttatga aaaaaataca tatttattgt cacaaataaa atcccctacc
      181 ggcaaatatt tgctgataag ggatttcatg catccaacat ctgaaacaac tttatactac
      241 ataaggcaat gtttttattt tacgcagtaa atatcttttc aaaatagtaa tatccatgga
      301 atcaatatat ccatcctcgt ttaaatcggc tgctttttta tcgtcagcta caggaaaatc
      361 gtcaatttta tgaagaatat atctttttaa cagagtaaga tctgttgaat ttatctcacc
      421 atcaccattt aaatcccctg gcaatcccaa cgtaaaggta ggtgtcgggg tcagctttga
      481 tgtcggtgtt ggtgtgggaa tcaccccttc cagaaaagca tccaatgcct ccgagggtct
      541 tccgtcatcg taccatgccc ctttaccgta ccctgcccaa ttatagcatt gcggctccca
      601 atagaatacc cccaatcctt tacctccaga taccgttctt gtcttattta taatatccgt
      661 taggaaagct ttgctcgtgg aagccgggta agttggcatt ccgacttcgc aaatcattac
      721 ttcctttccg tatctcgata ccatatcatt catatttgaa agacactgat tacttaagct
      781 ggaccagtta tcaacctcgg gatataggga cattccaatt acatcaaact tcgccccgtt
      841 atcgcgaaga ccgtcgaaaa tccatctaaa aaagctgtta ttatgcccgt tggatatatg
      901 gacaattact tttgcatcgc tgaaaacagc cttgacagcg tcatatccgc tgctaactaa
      961 aagtgcaaag ttcttcatgt ttttagaggc tttgccttct tcccataaca tcccgtcatt
     1021 ggtttcgttg cctacctgaa cccattcggg cttaacttcg ttggatttta atgcactcaa
     1081 cacatcatag gtatggttat agacatctgt cacgagccga tttatatcat gattgttcca
     1141 tgccgccggt ttatactgct tccccggatc tgcccatgaa tcactgtaat gaaaatcgat
     1201 cataattcta aatcccatat ccgcacaacg ctttgctatc ttaacagtat cttccttgct
     1261 gcaatgaccg gaataagggt cgtcggacgg gttaacccac acccttagcc tgatagaatt
     1321 aattccatgc tctttcaata tttgaagaca atctttagca acaccgttat cgtcataaaa
     1381 cttatatccc tgctcttcca tctgcaaaag ccagcttaca tccgctcctt ttgcaaaatc
     1441 tgcagcctta accggggcag gattgacaaa aaatgcatat ataaataata agcacaggat
     1501 aaatgaaatt gttttcttca ccaaataata agcccccctt aaaaaaaatg aatgctaagc
     1561 tctcccaata tatataatac gaacaataaa tctttttaag gggcttattc taactttcta
     1621 ctatccatgt tatcgaccgg ctaatacact ccgttttacg gaaaaacatt tttgattacg
     1681 gctagtttcg aaggctatga ataggtgcgg gtatccttcc ccctctttca ataaactcct
     1741 tactgctgaa tttgctaacc ttcattaccg ggccagaacc taaaagtcca ccgaactcaa
     1801 ccacatcacc gaccttttta cccggtgcag gaattatccg aaccgctgtc gtcttattgt
     1861 ttattacgcc tatggcagcc tcatctgcta taatcgcaga aattgtttct gcactcgtat
     1921 caccgggaac tactatcata tccaaaccca cagaacaaac acatgtcatt gcctcaagct
     1981 tttcaatcga caacgccccg gccttaaccg catcaatcat acccgcatct tcacttacag
     2041 gaataaatgc accgcttaag ccgccgacat aggaagaggc cattgttccg cccttcttta
     2101 ccgcatcatt aagaagagcc agtgcagcag tggtaccatg agttccacac ctttcaagac
     2161 ctatttcctc caatatgtgg gcaacactgt cccctaccgc aggggttggt gccagcgaca
     2221 aatcaacgat accaaaggct actccaagcc gcctcgatgc ctcataggca accaattgcc
     2281 ccatccttgt tattttaaaa gcggtctttt taatggtctc cgatacggtt tcaaaatcag
     2341 cacccttaac cttttccaag gctgctttaa cagtacccgg accgctgacc ccaacgttta
     2401 taacacattc cggttcccct actccatgaa aagccccggc cataaaaggg ttgtcctcag
     2461 gtacgttagc aaatacaacc agttttgcac aggccatgcc tccgctgtct gctgtaagct
     2521 cggcagattt ttttatgatc cgtcccattt ctttaacagc atccatattt atgcctgatt
     2581 ttgtagtggc aaggttaacg gaagagcaaa cctttttggt gctgctaagg gcttccggaa
     2641 tggaagcaat aagctttttg tcccctaccg tatatccctt atggaccaat gcggaaaaac
     2701 cgccaataaa attaacacca acggtgtccg ccgccctgtc caaagttttt gcaaactcca
     2761 cataattctc atcgtcacag ctttccgcaa taatggaaac tggtgtaaca gaaattcttt
     2821 tgttaactat cggaactccg tattcctttt ctatgtcttc tccaaccttc accaggtttt
     2881 gtgcaagcct cgttatcttg tcatatattt ttcttctcga ttccttgccg ttcgaacatg
     2941 cacaatccct tagggatatt cccatggtga ttgtccttat atcaaggttt tcctgctgaa
     3001 ccat
//
LOCUS       DAOOJC010000309         3004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_25332_length_3004_cov_3.118345, whole genome shotgun sequence.
ACCESSION   DAOOJC010000309 DAOOJC010000000
VERSION     DAOOJC010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25332_length_3004_cov_3.118345"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..869)
                     /locus_tag="PLA01_08215"
     CDS             complement(219..869)
                     /locus_tag="PLA01_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HPD01316.1"
                     /translation="MNKEWSEMNKQMQVLIGKKATYKDGTELLIKLRNELFEQITQIV
                     NEYPAEAFYQMPYANAKGYHSKTLSYSIWHIFRIEDIVAHILIAGDEQILMAGGYQKK
                     IGSPVITTGNELQGQEIAEFSKKLNVRALYEYAKEVMLSTNSLLTSMEYSQMKTKYSD
                     EDKKRLIDSGCVSPDENARWLIDYWCGKDIIGLIKMPFSRHWIMHVEAMLRIKNKL"
     gene            complement(1040..1249)
                     /locus_tag="PLA01_08220"
     CDS             complement(1040..1249)
                     /locus_tag="PLA01_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPD01317.1"
                     /translation="MSIIIRVDRMMADRKMSLNELADKVGMTNVNLSNLKNGKMKGIR
                     FETMDAICKALRCQPGDLFEYKEED"
     gene            complement(1260..1742)
                     /locus_tag="PLA01_08225"
     CDS             complement(1260..1742)
                     /locus_tag="PLA01_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009986647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01318.1"
                     /translation="MKDKKAESLKKRIFGDMICSFAIAAVSIYVFITEMWTYFSTDRT
                     YPKHITFGLYALFVFGFTLITGLILLEIRKTGKPFSQKIINKLRVLAVLVIVAGLIPD
                     AVTPIIDDMLNGSATQVAYKLVFSEKNIIIAIVGVVIGIVSEMFVYGYELQDDIDSIA
                     "
     gene            2037..2243
                     /locus_tag="PLA01_08230"
     CDS             2037..2243
                     /locus_tag="PLA01_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01319.1"
                     /translation="MSGVSAALGAPGGWAMAATIPADIVQYYGYTLRAMQKLLYLYGF
                     SEIESDEENEFYNLIINGEIVETN"
     gene            complement(2480..>3004)
                     /locus_tag="PLA01_08235"
     CDS             complement(2480..>3004)
                     /locus_tag="PLA01_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01320.1"
                     /translation="FDPWLFLGSFTSYSSKEIMVSLPVGGSGALTYYYVISFYNNNAE
                     YMLGPEQFVDLVQTLEFEVKYMDNYRVLVRSKKLNQSYIIDVNDSKEFYEGRVYNKEG
                     KLIKPLDGFIIYQPHLYPVKTDGFGPYKLQAQNAIAGTSHADRLGYTVTYWKYNRQSK
                     TWVLDPEYFYTMLG"
BASE COUNT          878 a          651 c          468 g         1007 t
ORIGIN      
        1 agggcttgca agcttcaacg acagtatcca gattatcctt ctttccggta tcaatcaaga
       61 tgcctgaatc tccatcagac acaatatagc aattaacgag gccacacttt attctttgta
      121 ccatactcat agcaatttcc ccctataatc acattttcgt caatagaaaa caaaacatat
      181 tactgctctt ttttacagct ttattggggg cagatttttc acaacttatt cttaatccgt
      241 agcatcgcct ccacatgcat tatccaatgc ctgctgaagg gcatttttat caagccgata
      301 atatccttac cgcaccagta atcgataagc cagcgggcat tttcatccgg actgacacaa
      361 ccggaatcaa tgagcctttt cttatcttca tccgaatact tagtcttcat ctgtgaatat
      421 tccatgcttg taagaagtga gttggtagaa agcataactt ctttggcata ttcatataaa
      481 gccctgacat tgagtttctt tgagaattct gcaatctcct gtccctgtaa ctcattgccg
      541 gtcgtaataa caggcgaacc tattttcttc tgataaccgc cggccataag tatctgctca
      601 tcccctgcaa tcaaaatatg agctacaata tcctcaattc gaaagatgtg ccagatcgaa
      661 tatgagagtg tcttactgtg ataacctttc gcatttgcat aaggcatttg ataaaatgcc
      721 tcagcaggat attcgttcac aatctgcgta atctgctcaa acagctcatt ccgaagttta
      781 atgagcagtt ccgttccatc cttatatgta gctttcttac ctataagaac ctgcatctgt
      841 ttattcatct ctgaccattc tttattcatt gtgcatcaaa cccccttttt tcagctcctt
      901 tatgcattga agccggatat ttatagattt gttttctaat ccttcaaaga acttcatgtt
      961 cacagctatg ctctgaggat aaaaacaagg ttaccgtcat tcgaaaatac ttctcaaaaa
     1021 gtctggcaaa atttgtttat caatcctctt ctttatattc aaacagatct cccggctgac
     1081 acctcaatgc tttacatata gcatccatag tttcaaatcg tattcctttc attttaccat
     1141 tcttcaagtt tgaaagattt acattggtca ttccgacttt gtcagccagt tcattcagag
     1201 acatttttct atctgccatc attctatcaa ccctaattat tatcgacatt ttttgcctcc
     1261 tatgcaatag agtctatatc atcctgcagt tcatacccgt atacaaacat ttctgagaca
     1321 attccgataa cgacacctac aattgcaata ataatattct tttctgaaaa taccaattta
     1381 taagctacct gagtagctga gccatttaac atatcatcaa taatcggtgt cacagcatcg
     1441 ggaataagcc ctgcaacaat taccaatacg gctaaaacac gaagtttatt aattatcttt
     1501 tgggaaaacg gtttaccggt ctttcggatt tcaagaagta tcaagccggt aattaaagtg
     1561 aatccaaaca caaagagtgc atataatccg aaagtaatat gtttggggta tgttcgatcc
     1621 gtagaaaaat atgtccacat ctctgtaata aaaacataga ttgagactgc cgctatagca
     1681 aaactacata tcatatctcc aaatattctc ttttttaagc tttctgcctt tttgtctttc
     1741 atagcacact ctcctcggga tttaaacttt taatttaatt atttaaagtt tattatagaa
     1801 ccagtttact atttgacaaa aagtctgtca ataaatattt aaaacattac tttaaatatt
     1861 tcaattttga ttataattta tattcttcaa tacaactgaa ttattcaagg ttttcagcag
     1921 cttcgtccat ttgctgctgt tcttcagcct tgtatcatcc tttatcattt atacttccct
     1981 tcacccgctt cattagcttt cttaagactt aagaatgcac atccgcagaa tgttatgtgt
     2041 ctggagtatc agctgctctt ggtgctcctg ggggttgggc aatggcagca acaattcctg
     2101 cagatattgt tcaatattat ggttacacac ttcgtgcaat gcagaaatta ctgtatttat
     2161 atggattttc ggaaatagaa tcagatgaag aaaacgaatt ttacaactta attataaatg
     2221 gagaaatagt tgaaaccaat taaagttttc gagccctcgg ccggtgattc tcaggctgac
     2281 actattgttc agtttgagtt cacccaccga aagcttccgg atctcttcat cgctcccgta
     2341 agaggggcca ttttcagata tcatcatacc taacttctct ttatttaatg cctatgtatc
     2401 taatttgctc tataaaaata tcaacgaaat accggatact atttatactt tgtttttaaa
     2461 acccattgca gttgcatact taccccagca tggtataaaa atactcaggg tctaaaaccc
     2521 aggttttact ctgcctgtta tatttccagt atgttactgt atacccaagc cggtcagcgt
     2581 gggaagttcc tgctatagcg ttttgtgctt gcagcttata tgggccgaaa ccatcggttt
     2641 tgactgggta aaggtgtggt tgatagataa tgaatccgtc caggggcttg ataagtttgc
     2701 cttctttgtt ataaaccctt ccttcgtaaa attctttact gtcatttaca tcgatgatat
     2761 aagattggtt aagttttttg ctcctgacaa gcactctgta attgtccata tatttgactt
     2821 caaattctag agtctgcacc agatccacaa attgttcagg accaagcata tattcggcat
     2881 tgttattgta aaatgatatc acataataat acgttagtgc tccacttcct ccaacgggaa
     2941 gacttaccat aatctccttt gaactatagc tggtgaagct gcccaaaaac agccatggat
     3001 cgaa
//
LOCUS       DAOOJC010000310         2996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_25424_length_2996_cov_3.350901, whole genome shotgun sequence.
ACCESSION   DAOOJC010000310 DAOOJC010000000
VERSION     DAOOJC010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25424_length_2996_cov_3.350901"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PLA01_08240"
     CDS             <1..394
                     /locus_tag="PLA01_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01321.1"
                     /translation="GIVISILLGVVLVLALIGFSFLTVYWFPAMVIDNLGVIAAFKKS
                     IEVSKSYYWPTVGLILLVLLVGGVISTFVAFPFALLPYVGVVISSVPTALCSFITIVF
                     VMMVYRDKTGKNQPQDDEIGGSAPYEFI"
     gene            complement(448..1557)
                     /locus_tag="PLA01_08245"
     CDS             complement(448..1557)
                     /locus_tag="PLA01_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HPD01322.1"
                     /translation="MKNKAVHKRLMLVLLIPLGYLLLYLSSLSHPLTENIYSKGLYKA
                     LSIPLNILFGYIPVSVAEIILISTVLILLFLIVRTIIRIIKSPSEKWSILGRAISNIV
                     AAASILYLSFILLWGFNYQRLPFKDIAGYDTQPTDINTLKHVCEALIQRTNELRTQVD
                     ENIDGIMKLSGGINDTLKRAHMGYNNIESKYPELTYKFGRPKGVLLSEVMSYLGIGGV
                     YFPFTTEANVNISLPSTSLPFTACHEMAHQIGFSREDEANYIAYLACKFHPDHDFQYS
                     GTLSAMIYATNALYQYSPEDYFELREKFSDGVARDVKASNNYWEKYETKIQDFSSSIN
                     NAYLKANMQNDGVRSYGRMVDLLIAEYLKGLAANG"
     gene            complement(1715..2395)
                     /gene="aat"
                     /locus_tag="PLA01_08250"
     CDS             complement(1715..2395)
                     /gene="aat"
                     /locus_tag="PLA01_08250"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HPD01323.1"
                     /translation="MPVYRLTSELIFPHPSLANEDGILAVGGDLSPKRLLLAYANGIF
                     PWFCEGDPIIWWSPDPRCVLYPDSINISRSMRKFLKKNLYKVTFDTCFEDVITMCSEL
                     RKDNTWITHDIIESYCNLHRLGFAHSVETWHEGSLVGGLYGVSLGKCFFGESMFSVMD
                     NASKTALITLSQRLLEMDFLLIDCQVHSKHLESLGAINIDRNAFFECLKKGLSFETMR
                     GSWQFLNE"
     gene            complement(2466..>2996)
                     /locus_tag="PLA01_08255"
     CDS             complement(2466..>2996)
                     /locus_tag="PLA01_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD01324.1"
                     /translation="DIFNVLLQIMDYATLTDNNGKKADFRNTILIMTSNAGAREVGKT
                     MIGFDNRNIDRSAISKEVDRIFSPEFRNRLDEIVVFNHINEDMAILIAKKAIKQFEEK
                     LTPKKIQLKVTARCYKWLAQKGLSSIYGAREILRIVQDKIKTYFVDEVLFGKLSKGGT
                     AVVDIVDGEIKVSSSS"
BASE COUNT          850 a          567 c          603 g          976 t
ORIGIN      
        1 tggtattgtg atttcaatac ttctgggagt tgtacttgtt ttagcattga tagggtttag
       61 ttttcttacg gtttattggt tcccggctat ggttattgac aatttgggtg ttattgccgc
      121 ttttaaaaaa tccattgagg tttcaaagtc ttattactgg ccgactgttg ggcttatatt
      181 attggtttta ttagtaggcg gggttatttc aactttcgta gcttttcctt ttgcattgct
      241 gccctatgtt ggagttgtta tttcaagtgt gccgactgca ttatgttcgt ttatcacaat
      301 tgtatttgtt atgatggtat accgggacaa aaccggtaaa aatcaaccgc aggatgatga
      361 gataggtggt tctgcccctt atgaatttat ttagattagg atctttatag cttgtttatt
      421 ctccaatttg agagatcttg aaaaggctta accgttagcg gctaagcctt ttaagtactc
      481 agcaataagt aggtcgacca ttcttccgta gcttcttaca ccgtcatttt gcatattggc
      541 tttcaggtat gcattgttta ttgatgatga gaaatcctgt attttggttt cgtacttttc
      601 ccagtaatta tttgaggcct tgacatcgcg ggctacaccg tcgctaaact tttccctcag
      661 ttcaaaataa tcctccgggc tgtattgata aagagcattg gttgcatata tcatagccga
      721 aagagtacct gaatattgaa agtcatgatc aggatggaat ttacaggcga gataggcaat
      781 gtaattggcc tcatcctccc tggaaaagcc aatttgatgt gccatttcat ggcatgcggt
      841 aaatggaagc gatgtactgg ggagggatat gtttacattt gcctctgttg taaagggaaa
      901 atagacgcct ccgataccta agtaggacat tacttcagag agcaatactc ctttgggtct
      961 tccgaatttg tacgtcaact ccggatattt ggattcaata ttattataac ccatatgtgc
     1021 tctttttaat gtatcgttta taccgccaga tagcttcatt ataccgtcaa tgttctcatc
     1081 aacctgagtt ctgagctcgt tggtacgttg gattaatgcc tcacacacat gttttagcgt
     1141 attgatatcg gttggctggg tgtcatatcc ggcaatatct ttaaagggaa gtctctgata
     1201 attaaagccc caaagaagta taaaggataa atataatata cttgctgctg caacaatatt
     1261 tgatattgct ctgcccaata tgctccattt ttccgaagga gattttataa ttcttattat
     1321 tgttcttact atcaaaaaca ataaaattaa gacagttgaa atcaaaataa tttctgcaac
     1381 agatacggga atatatccga aaaggatgtt tagaggtatg ctaagtgctt tatataaacc
     1441 cttggaataa atattttccg tcaaaggatg ggaaagggat gaaagatata acaatagata
     1501 ccctaagggt atcaggagaa caagcattaa tcttttatgg actgctttat ttttcataat
     1561 tttcaccgac tttcatacgg gcttatattg tttaagtgaa gagaaatatg gaagaaacat
     1621 tatggagcaa gttgtagata ctgttaagtt aatacggcaa tactttcttc actctaggta
     1681 tctatataaa cagaatgagc aaattaaaat ctttttattc gttcagaaac tgccagctgc
     1741 cccgcatggt ttcgaaactg aggccttttt ttaagcattc gaagaatgca ttccggtcaa
     1801 tatttattgc acctaaggac tcgagatgct ttgaatggac ctggcaatcg attaatagaa
     1861 aatccatttc caaaagccgt tgggaaagtg taataagtgc ggtttttgaa gcattgtcca
     1921 taacggaaaa catggattct ccaaaaaagc attttccaag ggaaacacca tacagtccac
     1981 ccacaagaga accttcatgc caggtttcaa cagagtgggc aaaaccaaga cgatgaaggt
     2041 tgcaatagct ttcaatgatg tcatgggtaa tccaggtgtt atccttgcga agctctgaac
     2101 acatggtgat aacatcctca aaacatgtat cgaaggttac tttatacaaa tttttcttta
     2161 agaatttacg catggaacgt gagatattaa tgctatcggg gtatagtaca catctgggat
     2221 caggtgacca ccatattatg ggatcacctt cacaaaacca tggaaaaata ccgttagcat
     2281 atgcgagaag aagacgcttt ggggaaaggt ctccgcctac agccaaaatc ccatcttcat
     2341 tggctaggga gggatgggga aaaatcaatt cgcttgtaag tctgtaaaca ggcattaatc
     2401 catcacccca atcttctctc tttttacaaa ctaaaaatct ttaaccaaag cttcgccttt
     2461 tagctttagc tagacgaact cacttttatt tcaccgtcca ctatatccac taccgcggtt
     2521 cctcccttgg aaagcttacc gaagaggacc tcatcgacaa agtatgtctt tattttgtcc
     2581 tgtacaattc ttaatatttc ccgggcaccg taaatagatg aaagaccttt ttgagcaagc
     2641 cacttatagc accgggcggt aaccttaagc tgtattttct tgggcgtcaa cttctcctca
     2701 aactgtttta ttgccttctt ggcgattagg atggccatgt cttcattaat atggttgaag
     2761 accacaattt catcaagcct gtttcgaaac tcaggggaga atatgcggtc cacttctttt
     2821 gatattgcac ttctgtcaat gtttctgttg tcaaatccta tcatggtttt accgacttcg
     2881 cgggctcctg cgtttgaggt cataatcagt atggtgtttc tgaaatccgc ctttttaccg
     2941 ttgttgtcgg taagggtggc ataatccatt atttgaagca gcacattgaa tatgtc
//
LOCUS       DAOOJC010000311         2957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_25873_length_2957_cov_3.119573, whole genome shotgun sequence.
ACCESSION   DAOOJC010000311 DAOOJC010000000
VERSION     DAOOJC010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25873_length_2957_cov_3.119573"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="PLA01_08260"
     CDS             <1..996
                     /locus_tag="PLA01_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase"
                     /protein_id="HPD01325.1"
                     /translation="DKNEFTFVTTKGFEPFDIKEIGYNLKGRKLFVYYQNNLFVFSFI
                     YDPTDTKYLSGKIMESIINSIEINGIGVKLETRTIPGKNEKFVEIMQSVKTLYTNALS
                     KEQNEVRLKNKYFPKNLDLTNMVRIEGGTFIDDEGDEITIKPFYVSKNLVTINEWNKY
                     SNDKIDINKYNTKYNINIKSEKYPAVFETEEKYGMVNIKDSLSLFKFCNSLSKSHGLD
                     EYYSFVENSYGTSTLFEHNNGFRLLSEYELKYILRKTKSEPYKNNARSNTIAPVGSSD
                     KNAFGIYDFDSNVAEVTDFDYVFKVKDSNQRLCGFRYAKDAENPVQELINDFFLD"
     gene            complement(1165..>2957)
                     /locus_tag="PLA01_08265"
     CDS             complement(1165..>2957)
                     /locus_tag="PLA01_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPD01326.1"
                     /translation="GYFPDQQKKATIAANCSTFYVVDKDQNIVYTGTAVSKYDTDTNE
                     TVYIADFSPVTSEGTYYLAVQGVGKSVDFKISSKVYDDPFKVSMLGMYLMRCGTQVQA
                     TYNGNNFSHAACHTQDAYLDYINGQHTTKDGTGGWHDAGDYNKYVVNAGITVGGMLLA
                     WEQFQDQLESIQLDIPEKNNSIPDYLDEIKYELEWLLKMQYPDGSGKVAHKLSTRNFG
                     GFIMPESEWEERFFVPWGSAATADFVAMLAKASRIYRPYDATFADKCIEAAKVSYAFL
                     KANPSNQPPNQNGFSTGAYDTTDKDDRLWAAAEMWETLGEEEYLQDFENSASSYSKKV
                     IPDFDWSDVQNLGMFTYLLSNRSGKNKSLENEIKSSLFSAADFLVQNSNNHGYGRTLG
                     SIYYWGCNGTVARQTMVLQVANDLSPNADYINAALDSLSHIFGRNYYNRSYVTGLGIN
                     PPMNPHDRRSGGDGIRDPWPGCLVGGGWPGAKDWVDIQDSYETNEIAINWNGALIYAL
                     AGFANLKDNPFDPEPTPTPEPEIIYGDVNDDGFVDSTDLTMMKRLVLRKLSSLPVEDA
                     ADLNLDGSIDSSDITIMKRFLLRKIDKLPI"
BASE COUNT          912 a          605 c          531 g          909 t
ORIGIN      
        1 gataaaaatg aatttacctt tgtaaccaca aaaggatttg aacccttcga cataaaagaa
       61 ataggataca acctgaaagg caggaaactc tttgtatact atcaaaacaa cttgtttgta
      121 ttctctttta tatatgatcc cacggatact aaatatctgt ccggcaagat catggaaagc
      181 attattaatt ctatagagat aaacggtatt ggcgtgaaac tcgaaacgag aacaattccc
      241 ggcaaaaacg aaaaattcgt tgaaatcatg caatccgtta agactctata cacaaatgcc
      301 ctttccaagg aacaaaacga ggttcgtttg aaaaataaat attttcccaa aaatctggat
      361 cttacaaaca tggttagaat cgagggtggt acctttattg acgatgaagg tgatgaaatt
      421 acaataaaac cgttttatgt tagcaagaac cttgtgacaa tcaatgaatg gaacaaatat
      481 tcaaatgata agattgatat caataaatac aacactaaat acaatataaa tattaaatcg
      541 gaaaaatatc cggcagtttt tgaaacagaa gagaagtacg gtatggtcaa tataaaagac
      601 agcctgagcc tttttaagtt ctgtaacagt ttgagcaaaa gtcatggttt ggatgagtat
      661 tactcctttg ttgaaaacag ttacggcaca tcaacactgt ttgagcataa caatgggttc
      721 cgcctgctta gtgagtatga actgaaatac atcttaagga agactaaatc cgagccttat
      781 aagaacaacg ccaggtccaa cacaattgca ccggtaggct catccgacaa gaatgccttc
      841 ggtatatatg actttgattc caatgtagcc gaagtgaccg attttgacta tgtattcaaa
      901 gtaaaggaca gcaatcaaag gctgtgcggt ttcagatatg caaaggatgc ggaaaaccct
      961 gttcaggaat tgataaatga tttctttctg gactagataa agtgaagaga aatatttcag
     1021 aaacttatgt ttcttccata tttttcttca ctttttttca aaagtgaaca ctttttatga
     1081 aaaaagctca ggaatccaat gatcctgagc ttttttacag ttataaaatc aaattagtta
     1141 ttttctatat tgttagttat tcttttatat tggcaattta tctatttttc tcaaaaggaa
     1201 ccttttcatt atggtaatat cggatgaatc tattgatccg tcgagattta agtctgctgc
     1261 atcttctaca ggcaatgatg atagttttct cagtacaagt cttttcatca tagtaagatc
     1321 tgttgaatct acaaatccat catcgtttac atccccatat ataatttcag gttcgggagt
     1381 gggtgtcggt tctggatcaa aaggattatc ctttaaattt gcaaaacctg caagagcata
     1441 aatcaatgca ccattccagt ttattgcaat ttcattggtc tcgtaactat cctgaatatc
     1501 aacccaatcc tttgctccgg gccatcctcc tccgacaaga catcctggcc acggatcacg
     1561 gattccgtct cctcctgaac gcctgtcatg cggattcatg ggaggattga ttccaagtcc
     1621 cgttacatat gacctgttgt aatagtttct tccgaatata tggctcagcg aatccaaagc
     1681 cgcatttata taatcggcat tcggggaaag atcgtttgca acctgcagaa ccattgtctg
     1741 ccttgcgaca gtaccgttgc atccccaata atatattgac ccgagagtcc ttccataccc
     1801 atggttatta ctgttttgta caagaaaatc tgctgctgag aaaagagaac tctttatttc
     1861 attttccaga gacttgtttt taccagatct attagacaat aaataagtga acataccgag
     1921 attttgaaca tcgctccagt caaaatcagg tataaccttt tttgaataac tggaagcact
     1981 gttttcaaaa tcctgaagat actcttcttc acccaaagtc tcccacattt ctgccgctgc
     2041 ccacaatctg tcatccttgt cggttgtatc ataagcacct gttgaaaatc cattctgatt
     2101 gggtggttga tttgacggat ttgctttaag gaaagcatag cttacttttg cagcttctat
     2161 acacttgtcc gcaaatgttg catcataagg cctgtatatt ctagaagcct tagccagcat
     2221 agctacaaaa tctgcagtag ctgcacttcc ccaaggtaca aaaaatcttt cttcccattc
     2281 actttcaggc ataataaatc cgccgaagtt tcttgtggac aacttatggg caactttacc
     2341 gcttccgtca ggatactgca tctttaaaag ccactccaac tcgtatttta tttcatcgag
     2401 gtaatccgga atggaattgt ttttttccgg gatatccagt tgaatagact ccaactgatc
     2461 ctgaaactgt tcccaggcta aaagcatacc gccaacggtt atacctgcat taaccacata
     2521 cttgttataa tcccctgcgt catgccatcc tccggtgccg tcttttgttg tgtgctggcc
     2581 gttaatatag tcaaggtaag catcctgtgt gtgacatgct gcatgggaaa agttattgcc
     2641 gttatatgta gcctgaacct gtgtaccgca acgcatcaaa tacatgccca gcatcgatac
     2701 tttgaaggga tcgtcatata cttttgagga aatcttgaaa tcaacactct ttccaactcc
     2761 ttgtactgcg agataatatg tcccttcact ggtaaccggc gaaaaatcgg ctatgtaaac
     2821 agtttcattg gtgtcagtat cgtacttgga tacagctgta cctgtataaa caatattctg
     2881 gtctttgtca actacataaa aggttgaaca atttgcagct atagttgcct tcttttgttg
     2941 gtcaggaaaa tatccta
//
LOCUS       DAOOJC010000312         2900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_26530_length_2900_cov_4.205975, whole genome shotgun sequence.
ACCESSION   DAOOJC010000312 DAOOJC010000000
VERSION     DAOOJC010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26530_length_2900_cov_4.205975"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..2438)
                     /gene="recJ"
                     /locus_tag="PLA01_08270"
     CDS             complement(147..2438)
                     /gene="recJ"
                     /locus_tag="PLA01_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPD01327.1"
                     /translation="MNIKVNILNEKTEIPKELLDACGGDELIARIFYNRGFKNPETVT
                     QMLNENLYKPTKTDEFPNIKRAVERISRAIDSGEKICVYGDYDVDGVTSTVTLVECLR
                     YFTPSVIYHVPDRFTEGYGMNEDVVLGFSQQGVSLIITCDCGISNVNEIAAAKQLGMD
                     VILTDHHTIPDELPPADVILNPKLLGEGHRARNISGCGMAYFLCLALLESKGLHDRAS
                     DFLDLLALSLIADVVSLNGENRYLLKKALPVLINSKRTGLKKLLDLAGKNGKLESEED
                     IAFQIAPRINAAGRMETARLPVELLLCRDPDRAQEMAEKIDLLNTERKRVQQSIVDEA
                     VEMVETKKKNKTILVLFNEFWHHGIIGIAAGRICELYRKPTILLSLKEDGKTVVGSAR
                     SIEEINIYELIKGCSGRLLKFGGHSQAAGLSLRKEVLDDFTAEIETAVENKYYIKDQI
                     SVDADMGLDLEEVTDELYKRLESAGPYGEGFEPPLFYTRGIRVVSDRKTEKNHHIMVL
                     EDQNQNRVPAVKWFGTDKSLEGKSFDVTYKIGRNSYKGNTSIQLTLGYMIEAVGESKV
                     LFTGNIKDERRLDLESILKKYPEAQLFYEGLESAFNLNNTADRYSVNKCRDLVFLSTP
                     VNTSVFREIIALANPENVIINFSVLPDYSFKGFVLSLLGVLKHIIKKWGGRAFVEEVS
                     LKLCVEENIVKSGLKFLMQSGMINYFLSENERSFFVFEGKGQPDKKVFAAKKNLMNAL
                     MEKKAYRDFILSMEADMFFEYLK"
     gene            complement(2439..>2900)
                     /locus_tag="PLA01_08275"
     CDS             complement(2439..>2900)
                     /locus_tag="PLA01_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPD01328.1"
                     /translation="RDDSVEIWDWKTHGGNSAEKARNERLEGEKLRKSLQTIVYLYVL
                     KEQLDKVALRDMDCSKIRMCYWQPDPPKVLAEINYSADMHETFGKTLQNRINAILEYD
                     YESFDKNMYKKSCKLCEFNWFCNNERIDYKAMEEDDDFLEELDWDSIEELT"
BASE COUNT          756 a          740 c          471 g          933 t
ORIGIN      
        1 ggatatctcc taattattta cttgtcttgc caactataat tatactctaa gtttatggat
       61 gcatcaaata aatatgaaga aaaatgttta tgcacccttt gctatttact atatatatga
      121 ataaataccg gctgttatgg attaaattat ttcaaatact cgaaaaacat gtctgcctcc
      181 attgataaaa taaaatcccg gtaggctttc ttttccatga gggcgttcat gagattcttt
      241 tttgcagcaa agaccttttt atccggttgt ccctttccct caaacacaaa aaaacttcgc
      301 tcgttttcac ttaagaaata atttatcatt ccgctttgca tcaaaaactt caaacccgac
      361 ttaacaatat tttcctccac acagagttta agggaaacct cctctacaaa agctctgccg
      421 ccccactttt ttattatatg cttcagcacc cccagcaagc ttaaaacaaa gcctttaaat
      481 gagtaatccg gcaataccga aaaattaata atcacgtttt ccgggtttgc aagggctata
      541 atctccctga acacagatgt attgacaggt gtggataaaa atacaagatc cctgcattta
      601 ttcacggaat acctgtctgc agtattattc aaattgaagg ccgactcaag tccttcatag
      661 aacaactgag cctcgggata cttttttaaa atgctctcta gatccagtct cctctcgtct
      721 tttatatttc cggtaaacag tactttcgac tcacccaccg cttcgatcat atagcctaaa
      781 gtcagctgta tgcttgtatt tcctttgtaa ctgttcctgc ctattttata ggtcacatca
      841 aagcttttgc cctccaagga cttgtctgtt ccgaaccatt ttaccgccgg caccctgttt
      901 tggttttgat cctccaatac catgatgtgg tggttttttt ccgttttcct gtcgcttacc
      961 acccttattc cccttgtata aaacaaaggg ggctcaaatc cttcgccata tggacctgcc
     1021 gactcaagcc ttttatacag ctcatctgtg acctcttcta aatcaagccc catatccgca
     1081 tcaacactta tttggtcttt tatataatac ttgttttcca cagcagtctc tatttccgcg
     1141 gtaaagtcat caaggacctc tttccttaag gaaaggcccg ctgcctggga atggccgcca
     1201 aattttaaaa gcctgccgga gcaaccttta atcaactcat atatgtttat ttcctcaatg
     1261 gatcttgcag aacctacaac agtttttccg tcctctttca gggaaagtaa tatggttggt
     1321 tttctgtaaa gttcgcagat ccttccggca gcaatgccga ttattccatg atgccaaaac
     1381 tcattaaaga gtaccaaaat tgttttgttc ttttttttgg tttccaccat ttccacagct
     1441 tcgtctacaa tcgattgctg tacccttttt ctctccgtgt tgagtaaatc gattttctct
     1501 gccatttcct gtgccctgtc cggatccctg cataacagca gttcaaccgg aagccgtgct
     1561 gtctccatcc ttcccgcagc atttattctt ggtgcaatct gaaacgcaat atcctcttcg
     1621 ctttcgagct taccgttttt tccggctaaa tccaaaagct tttttagtcc cgttctcttt
     1681 gaatttatta acacaggcaa tgcctttttc agaaggtatc ggttttcccc gttaagactc
     1741 accacatccg caattaacga aagggccagc aagtctagaa aatccgatgc cctgtcatga
     1801 agtcccttgc tctccaacaa ggcgaggcag agaaaatacg ccataccgca accggatatg
     1861 tttcttgccc tgtgtccctc gcccaaaagc ttgggattaa gtataacatc ggccggaggc
     1921 aactcatcgg gaatggtgtg atgatcggtc aaaatcacat ccatacccag ttgttttgcc
     1981 gcagctattt cattcacgtt ggaaataccg caatcacagg ttattattaa agaaacccct
     2041 tgctgtgaaa accctaaaac cacatcctcg ttcattccgt aaccttcggt aaaccggtcg
     2101 ggaacgtgat agatgaccga aggagtaaaa tacctcaagc actcaacaag ggtaacagta
     2161 cttgtgactc cgtcaacgtc ataatcaccg taaacacata ttttttcacc actgtctata
     2221 gccctggata tcctttccac cgccctcttt atattgggaa attcatcggt tttagtgggc
     2281 ttatagaggt tttcgttgag catctgggtt accgtctccg ggttcttgaa gcctcggtta
     2341 taaaagattc ttgcaataag ttcgtctccc ccgcaggcat ccagcaactc ttttggaatt
     2401 tcggttttct cgtttaatat attcaccttg atattcattt atgtcaactc ctcaatgcta
     2461 tcccagtcca gttcctccaa aaagtcatcg tcctcttcca tagccttata atcaattcgc
     2521 tcattattgc agaaccagtt aaactcacat agcttgcagc tttttttgta catattcttg
     2581 tcaaagcttt cataatcgta ctccaaaata gcgtttatcc tgttttgaag ggttttcccg
     2641 aaggtttcat gcatatccgc actatagtta atttccgcta aaaccttggg tgggtcgggc
     2701 tgccagtaac acatccttat tttagagcaa tccatatccc ttaaagccac cttatcgagc
     2761 tgttccttca gcacatacaa atacactatg gtctgaagac tctttctcag cttttcaccc
     2821 tccaaccttt cgtttcttgc tttttcagca ctattccctc catgagtctt ccaatcccat
     2881 atttcaacac tatcgtccct
//
LOCUS       DAOOJC010000313         2889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_26667_length_2889_cov_3.706069, whole genome shotgun sequence.
ACCESSION   DAOOJC010000313 DAOOJC010000000
VERSION     DAOOJC010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26667_length_2889_cov_3.706069"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1693)
                     /locus_tag="PLA01_08280"
     CDS             complement(<1..1693)
                     /locus_tag="PLA01_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPD01329.1"
                     /translation="MDSKTKTVSRKVQKNSVKRIVRNIFRVIFKFIKLVFILGVLVSC
                     LVGGILTGMTVAYVSTAEELTQDQIMLTGFTTRVYDCNGNEIMALHGDKNREKVEFKD
                     IPQDLKDAFVAIEDKRFYDHPGIDVKRIASAVKNFFIPGASSHGGSTITQQVIKNLTG
                     NEDRSVKRKVQEWWLALHLERKLSKDDILELYLNLIYMGQNCYGVQAAAQTYFDKDVS
                     ELTLAECASLAGITNLPGKYDPFLKQGKENNIKRQKVILKEMLIQEKITQEEYDKAVK
                     EELLFAESNKRSSESASNQPYFIDQVVIDVKNDLIKLGYSEELALKTIYNNGLRIYTT
                     MDPKIQQAMDEVFQNEEYFTKINKKTSQTPQAAMVIMDGNGYVRALYGGSGKKIGAPL
                     NRASDPRVQRQPGSTFKPIAVYGPAINERKITAATVIDDIPMYFQGTDKSPYPLNDNR
                     AYAGLTTIRNALRRSVNVVAARVWQEILGPDLSLKYLDKVNINRDDERYLSLALGGPR
                     EGVNTLQLAAAYVPFMSKGIYHEPITYTKVLDMNGNVIIEKKPENQEAAPVAQPEK"
     gene            1887..2579
                     /gene="yunB"
                     /locus_tag="PLA01_08285"
     CDS             1887..2579
                     /gene="yunB"
                     /locus_tag="PLA01_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HPD01330.1"
                     /translation="MRKKTNHYRRLWYFKRNRKKIRFYIILAAIFIILVVLIFLAESK
                     IRRSVMEFSEYKTKSIINNVVSNAVNDNFPDSINYEDIIIVNRDANNRVSSIQTDIVR
                     LNRIFANLSLDIQENLSELKNQRISIPLGVVFGNSVLSAEGPNISVKVIPSGSVETDF
                     KSEFTGAGINQTRHRIYLEVKTKVGIVIPFTKKDTEIITCIPVAETVIIGDVPYYYRG
                     SNEDLYIDTHGY"
     gene            complement(2619..>2889)
                     /locus_tag="PLA01_08290"
     CDS             complement(2619..>2889)
                     /locus_tag="PLA01_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPD01331.1"
                     /translation="IGKDAVVNGNIFAKNIFVSGIVKGNIEARETLKILSTAKLYGDI
                     LVNSLLTEEGSIFEGKCKMTAGTSNTENAKKSDIKKNDADKNKKA"
BASE COUNT          818 a          581 c          535 g          955 t
ORIGIN      
        1 gtttttctgg ctgggcgact ggtgccgcct cttggttttc cggtttcttt tcgattatga
       61 catttccgtt catatcaaga accttcgtat acgtaatagg ttcatgatat attcctttac
      121 tcataaaagg tacatatgct gccgccaact gcagcgtatt gactccttct ctgggtcctc
      181 ccaaagcaag ggaaagatat ctttcatcat ccctgtttat gttgaccttg tccaagtatt
      241 taagggatag atccggtccc agtatttcct gccataccct ggctgccacg acatttacag
      301 accttctcaa agcgtttcga atggttgtaa gcccggcata tgcacgatta tcattcaatg
      361 gatagggact tttatccgtt ccctgaaaat acataggaat atcatcaatt actgtggcag
      421 cggttatctt tctttcatta atagcaggac catacactgc tatgggcttg aacgtagatc
      481 ccggttgcct ttgtacccga gggtcagatg ccctgttcaa gggtgcacct atctttttcc
      541 cggagcctcc atatagtgca cggacatatc cgttgccatc cattataacc atagcagcct
      601 gtggagtctg gctggttttt ttgtttattt ttgtgaaata ctcttcattc tgaaatactt
      661 cgtccatggc ttgttgaatc ttcggatcca tggtggtgta tattctgagc ccgttattgt
      721 aaatggtttt caaggccaat tcctctgaat aacccagctt aataagatca ttttttacat
      781 cgataaccac ttgatcaatg aaataaggtt gattactggc agactccgaa gaacgtttgt
      841 tggattctgc aaaaagaagt tcttctttaa cagccttatc atattcttcc tgagttattt
      901 tttcctgaat caacatctct tttaagatta ctttttgacg tttgatattg ttttctttac
      961 cctgctttaa aaagggatca tattttccgg gtaagttggt aataccggca agagaagcac
     1021 attctgcaag ggtgagttcc gagacgtctt tatcaaagta ggtttgtgcc gcagcttgta
     1081 cgccgtagca gttttgaccc atatatatga ggtttagata aagttcaagg atatcatcct
     1141 tactcagctt gcgttctaag tgaagggcaa gccaccattc ctgaaccttt cgttttacag
     1201 agcggtcttc gttaccagtc aggtttttta ttacctgctg tgtgatggtg cttccgccgt
     1261 gggatgaggc tccgggaatg aagaaattct ttacggcact ggctattctt tttacgtcta
     1321 tacccgggtg atcataaaac cgtttgtctt ctattgcaac aaaggcatcc tttaaatcct
     1381 gaggtatgtc tttaaactca accttttcac gattcttatc tccatgcagg gccataatct
     1441 cattaccgtt gcaatcgtat acccgtgtgg taaatcccgt aagcataatt tgatcttgag
     1501 tcagttcttc agcagtactg acataagcga ccgtcatgcc ggtcagaata ccgccgacca
     1561 gacatgagac taaaacaccc aaaataaata caagtttaat aaacttgaat atgactctga
     1621 atatgttgcg gactattcgt ttaacgctgt ttttttgaac ttttcttgaa acagtttttg
     1681 ttttagagtc cataaaagtt cctctccttt gtaataaaga aaactaatca aaatcagtat
     1741 ttctttctta tgtacaattt aaatttttgt ctaaacttat ttcattataa cataaaaagc
     1801 aatgatgatt aagagtttta tcaatacata gaaaaagtat cacaaaggac aggccatgaa
     1861 cataatataa gtaaggaggg ccttttatga gaaaaaaaac caatcattac cgccgtttat
     1921 ggtattttaa acgaaaccgg aaaaaaatac ggttttatat aattcttgct gctattttta
     1981 ttattcttgt tgtccttatt tttcttgccg aatcgaaaat aagacgcagt gtcatggaat
     2041 tttcggaata taaaacaaaa tccataataa acaatgtagt aagtaatgcc gttaatgaca
     2101 actttcccga cagcataaac tacgaagata ttataattgt aaacagggac gcaaacaata
     2161 gagtcagctc aatacagacc gatatagtaa ggttaaacag aatttttgcc aatttatctc
     2221 ttgatattca agaaaacctc tcagagttaa aaaaccaaag aatatcaatt cctcttggtg
     2281 tagtattcgg caattccgtg ctttctgccg aaggaccgaa tatcagtgtc aaagtaatcc
     2341 cctcaggcag tgtggaaacc gattttaagt cggagtttac cggtgcaggt attaaccaga
     2401 caagacatag gatatatctt gaggttaaga ccaaggtagg tatagttata ccttttacaa
     2461 aaaaggatac cgaaataata acatgtatac ctgtggctga gacggtaata attggcgatg
     2521 tcccatacta ttacaggggt tcaaatgaag atttatatat cgatacccac ggatattagc
     2581 accgccaatc agggcaaaga ctgatatatt gcggtttttc aagctttttt attcttatcg
     2641 gcatcgttct tttttatatc gctttttttg gcgttttcag tattggaagt tcctgccgtc
     2701 atcttacatt ttccttcaaa gatgctgcct tcttcagtta ggaggctgtt aaccaatatg
     2761 tctccgtaaa gctttgccgt agacaagatt tttaatgttt cccttgcttc aatgtttccc
     2821 tttactatac cggaaacaaa aatgttcttt gcaaatatgt ttccgttaac aacggcatct
     2881 tttccgata
//
LOCUS       DAOOJC010000314         2878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_26824_length_2878_cov_2.656040, whole genome shotgun sequence.
ACCESSION   DAOOJC010000314 DAOOJC010000000
VERSION     DAOOJC010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26824_length_2878_cov_2.656040"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..600)
                     /locus_tag="PLA01_08295"
     CDS             complement(91..600)
                     /locus_tag="PLA01_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPD01332.1"
                     /translation="MIIDIANILKSDGASLDIELNEPLEKDTFVVGDFIVDRPISFKG
                     RMVNAGGVIKLDGRLVAEYRTNCFRCLKDIESMVQVDIQEDFIEEGSDKTDEHYTYQG
                     KYVDLEKALKDNIVLNLPVRQLCDYECKGFCPKCGVDLNSSECKCHSDDIDPRMKALK
                     DFFKDSVNN"
     gene            complement(792..1988)
                     /locus_tag="PLA01_08300"
     CDS             complement(792..1988)
                     /locus_tag="PLA01_08300"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD01333.1"
                     /translation="MLCIAWYDLIRLLRDRTTLLIIMVIPIILTVVMGFAYSSGGSDE
                     TPKIPVGIAADDHSDFVRELIERIKQDSSIKAIEMSRQEIIEGIKESELQAGFIIPEG
                     FTENISEGKAGIHVLKQPASTGYVVAQQVIMTAFTGLNIKEASKVYFDNFLESSNMEN
                     RDLIISRITERFEKKLEGPSTVTVEDIRVSGGEESLDFDGMAQSSIGVTVMFVMFTVI
                     LGVGVILEDKKNRTWERLSATPAGTATIMMGKVLGIFLKSWIQVGILILFGRFVLGVG
                     WGNSLHASILLISVYLLCLTTLGMLLSTIVKTNAQLAAYSSVLIISTSMLAGCYWPLE
                     IVPEFMQKAAMIFPQYWAVKALNNTVGTNMGLGSILPHITVLVFMAGVFFIASAAVEK
                     VRVKNC"
     gene            complement(2149..>2878)
                     /locus_tag="PLA01_08305"
     CDS             complement(2149..>2878)
                     /locus_tag="PLA01_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD01334.1"
                     /translation="EKSKEFPPDFMSVMFDMQQKTGEGILEFYEDIKSYKEGHEKPET
                     VSGMQYYSAAMLVMFILFGASTGTKLMVEERESNTLGRLLTTGAGKVSLITGKFLGLL
                     LITFTQAMILIVFTRFAYGVDWGSSIAGVVIITVCAAFSGSGLGMMIGSMAKTSSGAE
                     GLSQLFIQTFTIIGGGMMPFYMMPEFIRVIAKGTLNWWAVNGYFDLMLGSGFTGILPS
                     CVVLVLMGVLYLGIGVARFKTQVS"
BASE COUNT          876 a          738 c          439 g          825 t
ORIGIN      
        1 tacacctcct catcccatac aacaatccat tgtatactaa gtaaaatctt gtactcatcg
       61 agggtactca attttgtaat ctaaattcca tcagttgttc acactgtcct taaaaaagtc
      121 ctttaatgct ttcatacgtg gatcaatatc atcgctatgg catttacact cgctgctatt
      181 aagatccaca ccacacttcg ggcaaaaacc cttacactca taatcacaaa gctgcctgac
      241 cggaagatta agcacaatat tgtcttttaa cgccttttcc aggtctacat acttgccctg
      301 atatgtataa tgctcatcgg ttttatcgct gccttcctca ataaagtcct cctgtatgtc
      361 aacctgaacc attgactcaa tatccttcag gcatcggaaa caatttgtcc tgtattcggc
      421 caccaacctg ccgtcaagct taataacgcc tccggcattt accatcctgc ctttaaagct
      481 tattggcctg tcaacaataa aatccccaac cacaaaggta tccttttcca acggttcatt
      541 aagctctata tcaagagatg ctccatcgct tttcagtata tttgctatat caattatcat
      601 actcatcacc tcttaaaacc gacagtgaat attatactaa ctaataattc acatgtcaag
      661 aattatgtga gaaccttttt tttattatca cacaaaagaa taatataata ctaaaattat
      721 gggtgcatta gaaaatatgc acccataatt ttgaatactt taaagcaatc agcccgatat
      781 gaatatatta ttcagcagtt tttaaccctt accttttcaa cggccgcaga tgctataaag
      841 aacactcccg ccataaatac aagaaccgta atatgcggca gtatggatcc aagtcccatg
      901 tttgtaccca cggtattgtt taatgcctta accgcccagt attgcggaaa aatcatcgca
      961 gccttctgca taaattccgg tactatttcc aaaggccagt agcatcctgc cagcatggat
     1021 gtcgatataa ttagtaccga agaataagct gcaagctgtg cgtttgtttt tacaatcgta
     1081 gaaagcagca ttcccagtgt tgtcagacac aaaagataaa cgcttatcaa aagtatgctt
     1141 gcatgcagag aatttcccca acctaccccc agcacaaagc gtccgaaaag tatcagaatg
     1201 cccacctgaa tccaactctt taaaaatatt ccaagaacct tgcccatcat aattgttgca
     1261 gtaccagccg gagtggctga aagcctttcc catgtcctgt ttttcttatc ctcaagtatt
     1321 acccccacac ccagtatgac cgtaaacatc acaaacatta cagtaacacc gatggaactc
     1381 tgtgccatcc cgtcaaaatc caagctttcc tcgcctcccg acacccttat atcctccaca
     1441 gtaacggtcg aaggtccttc aagctttttt tcaaacctct cggttatccg tgaaataatc
     1501 aaatccctgt tctccatatt cgatgactca agaaaattat caaaatacac ctttgaagcc
     1561 tctttgatgt ttaatccggt aaaggcagtc atgataacct gttgtgccac cacatacccc
     1621 gtagaagctg gctgttttaa aacatgtatt cctgcctttc cttcactgat attttcggta
     1681 aagccctcag gaataataaa accagcttga agttccgatt ccttaattcc ttcaataatc
     1741 tcctgccggc tcatttcaat ggcctttatg ctgctgtctt gttttattct ttcaataagc
     1801 tcccttacaa aatcactatg gtcatcagca gcaattccca caggaatttt gggagtctca
     1861 tccgagccac cgctgctata tgcaaatccc attactaccg ttagaattat aggaataacc
     1921 attataatca aaagggttgt ccggtccctt aatagcctta tcagatcgta ccatgcgata
     1981 cataacactc tcaatcgttt cgcctcctta taaaacatac tgcaggattc caggaaagac
     2041 ataccgaaaa cttaagtttg gctatttgtt cacattgtct cttccatgct ttcctttttt
     2101 aaataataaa catcaaaaaa atatacatcc ggcttaatac aatgcggttt aggatacctg
     2161 cgtcttaaac cgtgccacgc ctatgccgag gtataaaact cccatcaaaa caagaaccac
     2221 acaggaggga agaataccgg taaaaccgct tcccagcata agatcaaaat atccgtttac
     2281 tgcccaccag ttcagtgtgc cctttgcaat aactcttata aattcgggca tcatataaaa
     2341 cggcatcatc ccgcctccga taattgtaaa tgtctgaata aaaagctggg acaagccttc
     2401 tgcccccgat gaggtttttg ccatggagcc tatcatcatt cccagccctg atcctgagaa
     2461 tgcagcacat acggtaataa taaccacgcc ggctattgaa cttccccaat ccactccata
     2521 tgcaaaccgg gtaaatacaa taagaatcat cgcctgagta aaggtaatga gaagaagccc
     2581 taaaaacttt cctgtaatga gtgacacctt tcccgctccg gtggtcaaaa gccttccgag
     2641 agtattcgac tcgcgttctt ccaccataag ctttgttcct gtggaagcac cgaacaaaat
     2701 aaacattaca agcattgcag cagaatagta ctgcattccg gaaacggttt caggcttttc
     2761 atgcccttct ttataagact taatatcctc ataaaactca agaatacctt ctcctgtttt
     2821 ctgctgcata tcaaacatta cagacataaa atccggcgga aattcttttg atttctca
//
LOCUS       DAOOJC010000315         2876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_26844_length_2876_cov_3.367246, whole genome shotgun sequence.
ACCESSION   DAOOJC010000315 DAOOJC010000000
VERSION     DAOOJC010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26844_length_2876_cov_3.367246"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PLA01_08310"
     CDS             complement(<1..608)
                     /locus_tag="PLA01_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HPD01335.1"
                     /translation="MSSIRAKFIRGEEVKFISHLDLMKMYERALRRSGIPIAYSQGFN
                     PHPQMVFGLPLSVGVTSESEYADFEMEGDVEPGEFVQRLNSNLPEGIRIVDAADKNSR
                     SNIMASVAGASYDILVSTDSDSDIGAFKELLRQFLDKEEIYIDKESKGKIKQVDIRPM
                     IYDIDMGIMEIDGLSKTTTEGHSDIRKCQNPWLLRYAGVIKE"
     gene            complement(601..2442)
                     /locus_tag="PLA01_08315"
     CDS             complement(601..2442)
                     /locus_tag="PLA01_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPD01336.1"
                     /translation="MGIRVCDRILQSVEKPSRYTGNEWNSIKKDIGTIDIRFAFCFPD
                     VYEVGMSHLGMKILYHLLNERKDVFCERVFAPWVDMEAKMREYGIPLFTLETHDAVKE
                     FDFVGFTLQYEMSYTNIINMLDLAGFPVLSANRTKNHPFVSAGGPCAYNPEPLADFVD
                     FFVVGEGEEVINEVMDAYLDWKKKDLSREDFLRSISKIEGIYVPQFYEVKYNPDGTIG
                     SFRPVNDMYPSKIRKRIVRDLDRVYYPDKMVVPFTGIVHDRVMLELFRGCIRGCRFCQ
                     AGFIYRPVRERSADKLLGIARNLVESTGYEEISLTSLSTSDYSSLKELTDGLIKDMEP
                     KKVNLSLPSLRVDSFSLELMEKAQKVRKSGLTFAPEAGSQRLRNIINKGVTEEDLLKS
                     VSTAFGGGWSGVKLYFMLGLPIENFEDIEGISDLGEKVVDAYMKNPKEKRGKGLNVTI
                     STSFFVPKPFTPFQWESQDSMEVYKQKQSFLKSKIKTRYIKYNWHDPELSFLEAVFSR
                     GDRKLSKVLLKAFEKGCKFDSWGEHFRFDKWMEAFDECGIDPHFYANRKRPYDEVLPW
                     DHIDTGVSKKFLQREYEKAERQEVTSNCRISCSGCGAAVFEGGICLE"
     gene            complement(2600..>2876)
                     /locus_tag="PLA01_08320"
     CDS             complement(2600..>2876)
                     /locus_tag="PLA01_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HPD01337.1"
                     /translation="TDEQLTTKNIIIQKVRNYGIKDDTAGRQNIDDVGSGEGYFITNG
                     KYIEITWSKTSRSERTKYMDKQGNEIVLNLGQTWVQIFPVNGSMNIE"
BASE COUNT          846 a          721 c          422 g          887 t
ORIGIN      
        1 tcttccttta tcacgccggc atatcttaac agccatggat tttggcactt tctaatatcg
       61 gaatgacctt cggttgttgt tttagaaagc ccatctatct ccatgatacc catatcgata
      121 tcatatatca tagggcgtat gtcaacttgc tttattttgc ccttggattc cttatcaata
      181 tatatttctt ctttatcaag aaactgcctt aatagctcct taaaagctcc aatatctgaa
      241 tcactatccg ttgaaaccaa aatatcatac gatgcccccg caacagaagc cataatgtta
      301 gatcgagaat ttttatcagc agcatcgaca atacgaatac cttcaggaag attgctgttc
      361 agcctttgca caaattcccc cggctcaaca tctccctcca tctcaaaatc agcatattcg
      421 gattcactgg taaccccaac agaaaggggc agtccaaaaa ccatctgcgg atggggatta
      481 aacccttgcg aataggctat tggaataccg gaacgccgca aagctctttc atacatcttc
      541 ataagatcaa gatgggaaat aaacttaact tcctcacctc gaatgaattt tgccctgata
      601 ctactcaaga catatccccc cctcaaaaac agcagcccca caacctgagc agcttatcct
      661 acagtttgag gtaacctctt gtctttcggc cttttcatac tctctttgca aaaacttctt
      721 cgatacgccc gtatcaatat gatcccacgg aagaacttca tcataaggcc tctttctatt
      781 ggcataaaaa tgaggatcaa ttccgcattc atcaaaggct tccatccatt tgtcaaacct
      841 gaagtgttca ccccagctgt caaatttgca tcccttctca aaagccttca agagaacctt
      901 tgacagtttt ctgtcccctc tggaaaaaac agcttccaaa aaactaagtt cgggatcatg
      961 ccaattgtat tttatatacc ttgtctttat tttgcttttt aaaaatgact gtttttgctt
     1021 atatacttcc atgctgtcct ggctttccca ctgaaacgga gtaaaaggtt tcggaacaaa
     1081 gaatgaggtg cttatggtaa cattaagtcc tttccccctc ttttctttcg ggtttttcat
     1141 gtaagcatcc acaacttttt cgccaagatc cgaaatacct tctatatctt caaagttttc
     1201 tatcggtagc cccagcataa aataaagctt taccccgctc catcctccac caaatgcagt
     1261 agaaaccgat tttagcaagt cttcttcggt aactcctttg ttgattatat ttcgaagcct
     1321 ctggctaccg gcttccggtg caaaagtcaa accgcttttc cgaaccttct gtgccttttc
     1381 cataagttcc agggaaaaag aatccaccct aagagaagga agagaaagat tgactttctt
     1441 tggctccata tccttaatca acccatcggt aagttctttt aaagaggaat agtcactggt
     1501 acttaaggat gtgagggata tctcttcata gcctgtactt tccaccaaat tccttgcaat
     1561 ccccaacagc ttgtccgccg acctttctct cacaggcctg tatataaacc cggcttgaca
     1621 aaacctgcac cctcttatgc accctctaaa caattcgagc attacccggt catgtactat
     1681 acctgtaaaa ggaaccacca ttttgtcagg gtaataaacc ctatccagat ccctgacaat
     1741 tcttttcctt attttgctag gatacatgtc attaaccggc cggaatgagc cgatagttcc
     1801 gtcggggttg tatttcacct catagaattg gggaacataa atgccctcaa ttttcgaaat
     1861 cgaacgcaaa aaatcttctc ttgacaaatc cttctttttc caatccaaat aagcatccat
     1921 gacctcattt ataacttcct ctccttcgcc caccacaaaa aaatccacga aatctgccag
     1981 cggctcggga ttataagcac aaggaccccc tgcactaaca aaaggatgat tctttgtcct
     2041 attagcactc aaaacaggaa atccggcaag gtcaagcatg ttaataatat ttgtataact
     2101 catttcatat tggagggtaa atccaacaaa gtcaaactcc ttaacagcat cgtgagtctc
     2161 aagggtaaaa agaggtatgc catactccct catctttgct tccatatcaa cccacggtgc
     2221 aaaaaccctt tcacagaaga catcctttct ctcgttaaga agatggtata aaatcttcat
     2281 gcccagatga gacataccta cttcataaac atcaggaaaa caaaaagcaa accttatatc
     2341 aattgttcca atatcttttt taatactgtt ccactcattg ccggtatacc ttgacggttt
     2401 ttcaacactt tgcaatattc tgtcgcatac tctaataccc aacgtaacct ccatataaat
     2461 tacaaaaaaa ttctggaatt atttatctac cacaatccag tttcaataaa ctgtataatc
     2521 tataaatata ataaccaatt ggcttctttt tatcaacact aacttaatta catatttaac
     2581 gggatacaat gtcccgatct cactcgatat tcatactgcc gtttacagga aatatctgaa
     2641 cccaagtttg tcctaaattc agaacaattt cattaccttg cttatccata tatttcgttc
     2701 tttcggatct cgatgttttt gaccatgtta tctcaatgta tttaccgttg gtaataaaat
     2761 atccttctcc gcttcccaca tcatcaatgt tctgtcttcc ggctgtatca tctttgatac
     2821 cataatttct taccttttga attataatat tctttgtagt aagctgttca tcggtc
//
LOCUS       DAOOJC010000316         2871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_26903_length_2871_cov_3.406605, whole genome shotgun sequence.
ACCESSION   DAOOJC010000316 DAOOJC010000000
VERSION     DAOOJC010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26903_length_2871_cov_3.406605"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..1637)
                     /gene="clpX"
                     /locus_tag="PLA01_08325"
     CDS             complement(342..1637)
                     /gene="clpX"
                     /locus_tag="PLA01_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPD01338.1"
                     /translation="MSRYDEKKQLKCSFCGKTQEQVKRLVAGPGVYICDECIELCSEI
                     IEEEFEEAKADADLNSVPKPSEIKDILDQYVIGQESAKKSLAVAVYNHYKRINADYKG
                     SDVELQKSNIVMLGPTGSGKTLLAQTLAKILNVPFAIADATSLTEAGYVGEDVENILL
                     KLIQAADYDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTIAS
                     VPPQGGRKHPHQEFIQIDTTNILFICGGAFDGIDKIIENRIGKKTMGFGAKIESPKKI
                     DIGEILKNILPQDLLKFGLIPEFVGRLPVIVSLHSLDRNALMQILSEPKNALVKQYQR
                     LFEMDDVILEFEKEALEAIADKAISRNTGARGLRAIIEEIMLDVMYDIPSQPNVEKCI
                     ITRDAVEGKGTPTVIMNENKKPLKKSMNKKSRVKKESAS"
     gene            complement(1654..2238)
                     /gene="clpP"
                     /locus_tag="PLA01_08330"
     CDS             complement(1654..2238)
                     /gene="clpP"
                     /locus_tag="PLA01_08330"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPD01339.1"
                     /translation="MSLVPIVVEQTNRGERSYDIYSRLLKDRIIVLSDEVNDVTASLV
                     VAQMLFLEAEDPDKDIQLYINSPGGSVTSGFAIYDTMQYVRPDVSTICIGMAASMGAF
                     LLAAGAKGKRFALPNSEIMIHQPLGGARGQATDIKIHAEQIIKIKNKLNKILSERTGQ
                     PIEKIERDTERDFFMSAEEAKAYGIVDEVMERRK"
     gene            complement(2309..>2871)
                     /gene="tig"
                     /locus_tag="PLA01_08335"
     CDS             complement(2309..>2871)
                     /gene="tig"
                     /locus_tag="PLA01_08335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514322.1"
                     /note="Tig; RopA; peptidyl-prolyl cis/trans isomerase;
                     promotes folding of newly synthesized proteins; binds
                     ribosomal 50S subunit; forms a homodimer; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPD01340.1"
                     /translation="KVKELPVIDDEFAKDVSEFDTLEDYKAELKKKLTDHKEHMAKHK
                     IEEEVIDKVTGNATVEIPEVMIESQIERLVQNLDARLRYQGLDLEKYIMIMGIDQNTL
                     REQLRPEAEKEVKTQLVLEKLYKVENITASEEELEEEIKSLAENYKQDVDDFKKHLKD
                     DDIEYIKTNLEIKKLVDFLVENAKIA"
BASE COUNT          746 a          697 c          424 g         1004 t
ORIGIN      
        1 cccgaggagt atttacaggc tctggccggc gaaacttaag tttctggaat atttttcatc
       61 actctgtgct taaggagcaa tatattttga gcactttctc aagtctggct tgcccgcatc
      121 ctccgtttga ggtgcataag attcaattta ttttggacac aaaaaagcca ggcaaattac
      181 ctggcccatc gtcgtttcct ctttatatta aggataaata ttaattttgt attaaggtta
      241 aatactaatt ttgtgaagaa aactgcggaa atgtcccagg caaactttcc ctcacaatta
      301 ttcaatgtat tcttttatta agaagctgat tccttcttat cttaagaagc tgattccttc
      361 ttaaccctcg atttcttatt catagacttt tttagcggtt ttttattctc attcattatt
      421 accgtgggag tgcccttacc ttcaactgca tcccgtgtaa ttatacattt ttcaacattt
      481 ggctgtgaag gaatgtcata cattacatcc agcataatct cctcaattat tgcccgaagc
      541 cctctggcac cggtatttct tgagatggcc ttatccgcaa tcgcctccaa agcctctttt
      601 tcgaactcaa gtatgacatc atccatctca aagagccgct gatactgttt tacaagagcg
      661 ttcttgggct cactcaatat ctgcataaga gcatttctgt caagagaatg caaggatacg
      721 ataacgggaa gtcttcctac aaactcagga ataagaccga acttcaagag atcctgagga
      781 agaatattct ttaaaatctc tccgatatcg atttttttag ggctttctat tttagcacca
      841 aagcccattg ttttctttcc tatcctgttt tctataatct tgtcgatccc gtcaaaggct
      901 ccaccgcaaa taaaaagaat gttagtggta tcaatctgta taaactcctg atgagggtgc
      961 tttcttcccc cctgcggagg cactgaagca atagtcccct caagtatctt gagcagggct
     1021 tgctgaacac cctctccgct aacatccctt gttattgaag gattctctga ttttcttgca
     1081 attttgtcaa tctcatctat atatattatt cctctttctg ccttttcaat atcgtagtct
     1141 gccgcctgaa taagttttaa gagtatattc tccacatcct caccaacata ccctgcctct
     1201 gtaagggatg ttgcatctgc tattgcaaac gggacattca atatttttgc aagggtctgg
     1261 gccaaaagcg tttttcctga acccgtcggt ccaagcataa ctatattgct tttttgaagc
     1321 tccacatccg accccttgta atctgcatta atcctcttat aatgattgta tactgctaca
     1381 gccaaagact tttttgcaga ctcctgacct ataacatact gatcaagtat gtcctttatt
     1441 tcactaggct tgggtacact gttcagatcc gcatcagctt tcgcttcttc aaactcttct
     1501 tcaattattt ccgagcatag ttcaatgcac tcatcacaaa tatacacacc gggcccagct
     1561 accagccttt tgacctgctc ttgagtcttt ccgcagaagg agcatttcag ttgcttcttt
     1621 tcatcatatc tggacatcca actaccacct catttatttt cttctttcca taacttcatc
     1681 gacaattccg taagccttag cttcctctgc agacataaag aaatctcttt ctgtatctct
     1741 ttcaatcttt tcaataggtt gccctgttct ctcactcaga atcttgttta acttgttttt
     1801 tattttaatt atttgttctg catgaatctt tatatccgtg gcctgacccc gggcaccgcc
     1861 taatggttga tgtatcataa tttcactgtt gggaagtgca aacctctttc cctttgcacc
     1921 ggcagcaaga agaaatgcac ccatgctggc tgccataccg atacaaattg tggatacatc
     1981 cggtctaaca tactgcatcg tatcatatat tgcaaaaccc gaagttacag aacctccagg
     2041 actgtttata tataattgta tatccttatc agggtcttca gcctccaaaa acagcatttg
     2101 tgccacaaca agacttgccg ttacatcatt aacctcatca cttaaaacaa taatcctatc
     2161 ctttaaaagc ctcgaataaa tatcatatga acgctcacca cggtttgttt gctctacaac
     2221 tattggaacc agactcatta tatcccctcc tgtcgaatta gtaatcttat atatcttttt
     2281 cccatattct cagcttatta aacccgattt atgctatttt tgcattttcg accaaaaaat
     2341 ctacaagctt tttgatttcc aaattagtct taatatattc tatatcgtca tcttttaaat
     2401 gtttcttaaa atcgtcaaca tcctgcttat aattttctgc caatgatttg atttcttctt
     2461 caagctcttc ttccgatgct gtaatatttt ccaccttgta aagtttttcc aataccagtt
     2521 gcgtcttaac ttctttttct gcctctggtc ttagctgctc tcttaaggta ttttgatcaa
     2581 tgcccataat cataatatac ttctccagat ccagcccttg ataacgcaat ctcgcatcaa
     2641 gattctgcac aagtctttct atctggcttt caatcatgac ctccggtatt tccacagtag
     2701 catttccggt aaccttatca atcacttctt cttctatctt atgcttcgcc atatgttcct
     2761 tatggtccgt aagcttcttt ttgagttccg ccttataatc ttccaatgta tcaaattcac
     2821 tgacatcttt tgcaaattca tcatctatga caggcaattc ttttacctta a
//
LOCUS       DAOOJC010000317         2858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_27066_length_2858_cov_3.430967, whole genome shotgun sequence.
ACCESSION   DAOOJC010000317 DAOOJC010000000
VERSION     DAOOJC010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27066_length_2858_cov_3.430967"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PLA01_08340"
     CDS             <1..361
                     /locus_tag="PLA01_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01341.1"
                     /translation="KQTEMYDFIVLDTSSDFTAEKTLMMSFCDKVVIVAAQDKVSCLK
                     LECFLGNIDCSDKNKFVFICNKYLSDKENWLIKGNLTGRCLMSEYIDEFEPEPDDINI
                     EYLSTNKQFQKLAYTFI"
     gene            379..630
                     /locus_tag="PLA01_08345"
     CDS             379..630
                     /locus_tag="PLA01_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HPD01342.1"
                     /translation="MDSVVIVVHIHRTGEKVDVQIPLDITANELAVALNQAFDLGINT
                     EGDMADCYLKSENPIALLKGDKTLREYGLRNGTVINITS"
     gene            646..>2858
                     /locus_tag="PLA01_08350"
     CDS             646..>2858
                     /locus_tag="PLA01_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HPD01343.1"
                     /translation="MVKDKYKIVIYGKNIYSEYALPDGDDSIVKIGTTKNCQVRFNKD
                     HFFDEFEFYLSKKDSRWQLNCDKGVYFTTDGVMKIYYKDMNHGDMVGVKYQNFDGEIF
                     KLNFFIDFDADEKNYERVIDVSNINKISIGGLDGCDIVLKDELIGNDTVTLKAIDGEY
                     YIIDNKTRYGVYINGKKAPDRTKLNNYDFFMIVGYSFYFKNKRLYTSKYGNIRINNLS
                     YTDTTEQLSALKYPKFNRNTRVKYVIPEEEIEILPPKPKTAPPKKNLILTLVPVLAMI
                     ALTIVLRGIMRGGSGFTFVIYSVASMTVGLVMSIVSMVMDKKQYEKDMQQRKEKYFEY
                     IGKKEEEIKKYREIEWKIAQKIYKPIDVNIQAVSDFSGDLYDRDIKDEDFLFVRVGTG
                     VCEAKCKVVYTKQEYKDTDDELLSIPEDVEKKYRYIANYPIVSKFSNSNAIGIVGERS
                     KLYGVLKNITLDICIRHFYNDVKLFYMFSENDCERFAWLRWLTHVNNDDIGVRNLIYD
                     EESRNTILEYLYKQLTSRESQSNSSDNPSFNTFFVVFVFDSKGISKHPISKYIESCNK
                     YGFIFVFFEEHKEFLPKGCTEIIRIDTDNSGELLLSENGSDISKFDYIPVTDRTAEEV
                     ALKLGSVYVDEVNLESELTKNISLFELLDIMTINDLDLSVRWSQSQVYRSMAAPLGVK
                     TKNQTVYLDLHEKKHGPHGLVAGTTGSGKSEILQTYVLSMATLFHPYEVGFVIID"
BASE COUNT          966 a          434 c          635 g          823 t
ORIGIN      
        1 taagcagaca gaaatgtacg actttattgt gctggatact tcttctgatt ttacagccga
       61 aaaaaccttg atgatgagtt tttgcgacaa ggtagtaatt gtagcggcac aggataaggt
      121 ctcatgtttg aagctggaat gcttccttgg caatattgac tgctcggata aaaacaagtt
      181 tgtatttata tgcaacaaat acttgtcgga caaagaaaac tggcttataa agggcaattt
      241 aaccggtcga tgtttgatga gtgaatacat agacgaattt gaaccagaac cggatgatat
      301 caatattgaa tatttatcaa caaataaaca gtttcagaag ctggcttata cgtttatata
      361 attgtaggtg aaataaacat ggatagtgtt gttattgtgg tgcatataca tagaactggt
      421 gaaaaagttg atgtgcaaat accccttgat atcacagcaa atgagttggc tgtggcacta
      481 aatcaagcat ttgatttagg tatcaataca gaaggggata tggccgattg ttatctaaaa
      541 tcagaaaacc ctattgcact gcttaagggc gataaaacct tgcgggaata tggcttgaga
      601 aacggtactg tgatcaacat aacgagttag gaagaggacg gggtcgtggt taaagacaaa
      661 tataagattg ttatttacgg aaaaaacata tatagcgaat atgctctccc tgacggtgat
      721 gactccatag ttaaaattgg aacaacaaaa aactgtcaag tcaggtttaa taaggatcac
      781 ttttttgatg agtttgagtt ttacttgtca aagaaggatt cgagatggca gcttaattgt
      841 gataaaggag tatactttac caccgatggt gtaatgaaga tttattataa agatatgaat
      901 catggagata tggtgggggt aaagtatcag aattttgacg gtgaaatatt taaattaaac
      961 ttctttattg attttgatgc cgatgaaaag aattatgagc gtgttataga cgtatcaaac
     1021 ataaataaaa ttagtattgg aggccttgat ggctgtgata ttgttttaaa ggacgagctt
     1081 atcggtaatg ataccgtaac cttgaaagca atagatggag aatactatat tattgacaat
     1141 aaaacaagat atggagtata cataaacggt aaaaaggcac cggatagaac aaaattgaac
     1201 aactatgatt tcttcatgat tgtagggtat agtttttatt ttaaaaacaa gaggctgtac
     1261 acatcaaaat atggaaatat caggattaat aacctgagct ataccgatac caccgaacag
     1321 ctgagtgccc ttaagtatcc aaaattcaac aggaatacca gggttaaata tgtaattcct
     1381 gaagaagaaa ttgagatttt accaccaaag cccaaaacag ctccgccaaa gaaaaatctt
     1441 attcttactt tggttcctgt attggcgatg attgccctta ccattgtttt aaggggtatc
     1501 atgagaggcg gttcgggttt tacctttgtg atttacagtg tggcaagcat gaccgtgggt
     1561 ttggttatgt caattgtcag catggtaatg gataaaaagc agtatgaaaa agatatgcag
     1621 caaagaaaag agaagtactt tgagtatata ggcaaaaaag aagaggaaat aaaaaaatac
     1681 agggaaattg aatggaaaat tgcccagaaa atatacaagc ccattgatgt caatatacag
     1741 gcggtctcgg attttagcgg ggatctgtac gacagggaca ttaaagatga ggatttcctg
     1801 tttgtaaggg taggtaccgg cgtttgtgaa gcaaagtgca aagttgtata cacaaagcaa
     1861 gagtataagg ataccgacga tgaattgctt tccattcccg aagacgtgga aaagaaatac
     1921 cgctatattg ccaattaccc cattgtatcg aagttttcca attccaatgc catcggtata
     1981 gtgggtgaac ggtcaaagct ttatggagta ttaaaaaaca ttacccttga tatatgcata
     2041 cggcattttt ataatgacgt aaaactgttt tatatgtttt cggaaaatga ttgcgaaaga
     2101 tttgcctggc ttcgttggct tacccatgtg aataatgatg atatcggtgt aagaaacctt
     2161 atttatgatg aggaaagcag gaacaccatt ttagaatact tgtacaaaca gctgacttca
     2221 agggaaagcc agtcaaattc cagtgacaat ccaagcttta acacgttttt tgtggttttt
     2281 gtttttgaca gcaagggcat aagcaagcac ccgatttcaa aatatattga atcgtgtaac
     2341 aagtatggat tcatctttgt cttttttgaa gagcataaag agtttcttcc gaaaggctgt
     2401 acagaaatta taaggattga taccgataac tcgggagaat tgcttttaag tgaaaacggc
     2461 agtgatatat ccaaatttga ttatataccc gtcactgaca ggacggcgga ggaagtggca
     2521 ttgaaactgg gttcggtata tgtggacgaa gttaaccttg aaagcgaatt gaccaaaaac
     2581 atatcattgt ttgagctttt agacatcatg accattaacg accttgacct ttcggtgcga
     2641 tggagccaat cccaggttta tagaagcatg gcggcacccc ttggagtaaa gacgaaaaac
     2701 cagacagtgt accttgacct gcatgaaaaa aagcatggtc cccatggatt ggtagccggt
     2761 acgacagggt ccggtaagag tgagattttg cagacctatg ttttatcaat ggctacgtta
     2821 ttccatcctt atgaggttgg atttgttatt atagactt
//
LOCUS       DAOOJC010000318         2840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_27292_length_2840_cov_3.295512, whole genome shotgun sequence.
ACCESSION   DAOOJC010000318 DAOOJC010000000
VERSION     DAOOJC010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27292_length_2840_cov_3.295512"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PLA01_08355"
     CDS             <1..619
                     /locus_tag="PLA01_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HPD01344.1"
                     /translation="VDKITTNHTFFMRESDHFDFFAKSVLPYWSSLLKEDKDLRVWSA
                     GCSSGEEPYTLAMIMADFFGTQKPFWDTRILATDISMEIINKAISGIYSNESIETVPK
                     EWRRKYFRKLDSNNSVIADNIKNDVIFRLFNLMNQRFPFKKKFHVIFCRNVMIYFDEK
                     TKMDLVNKFYEFTEPGGYLFIGHSESLNRQETRYKYIMPAVYRKE"
     gene            624..1649
                     /locus_tag="PLA01_08360"
     CDS             624..1649
                     /locus_tag="PLA01_08360"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HPD01345.1"
                     /translation="MRKIRVLIVDDSLLFRETLARKISQDSAIEVVGTAFDPYSARDK
                     IIELHPDVMTLDVEMPRMNGIEFLRQLIPQYPIPVVVVSSVSESVFDALKAGAVDFVT
                     KPDMSNPKGVEAVINELIIKIKIASTAKVGNGKNESLLMKSKINTSDRSLNKVIAIGA
                     STGGTEAIFNIIKYFPKDMPGIVIVQHMPPVFTRLYAQRLDNSCLMEVKEASNGDMVC
                     PGRVLIAPGEYHMKLKKKDGKYFVECTKGQRVNGHCPSVDVLFNSVAETAGMNAIGII
                     LTGMGSDGAKGLLAMRKKGARTLGQDEKSSVVYGMPKVAFEIGAVEKQASLEAIPQLV
                     YSLLKKD"
     gene            1802..2209
                     /locus_tag="PLA01_08365"
     CDS             1802..2209
                     /locus_tag="PLA01_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD01346.1"
                     /translation="MRILIAEDDFASRKFLYKYLSSFGECDISIDGIEAVDSFIMALE
                     EGNPYDLICLDNMMPKLDGTRALKAIRDVEKQKGIEEGGRVKIIMTMALSNTSKVCSS
                     FDIRCEAYTVKPIDEKKFVDVLKRLGLLENCHK"
     gene            complement(2377..>2840)
                     /locus_tag="PLA01_08370"
     CDS             complement(2377..>2840)
                     /locus_tag="PLA01_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPD01347.1"
                     /translation="DKRGRRYYTPELANIMYKIKTMRDEGLEIKAIKKVLESENIIGG
                     PPPVVDQNNIVPVTAVKADSTPAEISRFFQEFSEQLTNNISLELESTKEHVVNEITKT
                     KLELGACFENNVRKLESKLDKHFDNVDRSIGMWREKNKTGFFKRLTKKIFK"
BASE COUNT          869 a          487 c          658 g          826 t
ORIGIN      
        1 ggtggacaaa ataacgacta accatacctt ttttatgagg gagtcggatc atttcgattt
       61 ttttgctaaa tccgtccttc cctattggtc ttcgcttctt aaagaggata aggatttaag
      121 ggtttggagt gctggttgtt ccagtggtga agaaccttat acccttgcga tgattatggc
      181 agattttttc ggcactcaaa aacccttctg ggatacaaga attcttgcaa cagatatatc
      241 gatggagata attaataaag cgataagcgg tatttattcc aacgaaagca ttgagacggt
      301 tccaaaagaa tggagaagaa aatatttcag aaaacttgac tcaaacaatt ctgtcatagc
      361 tgacaatatt aaaaatgatg tcatattcag attgttcaac cttatgaacc aaaggtttcc
      421 ttttaaaaag aagtttcatg ttatattctg cagaaatgtg atgatatatt ttgatgagaa
      481 aacaaaaatg gacttggtaa acaaattcta tgagtttact gaaccggggg gctatctgtt
      541 tatcggtcat tcagagtctc tgaaccgtca ggaaaccaga tacaaatata ttatgcctgc
      601 agtatataga aaggaataac aatatgagaa aaataagagt tctgattgtt gatgattctt
      661 tattgttcag ggaaaccctt gctagaaaaa tatcccagga ttccgctatt gaggtggtgg
      721 gaacagcttt tgatccgtat tcggcaagag ataagattat tgagctgcat ccggatgtta
      781 tgactttaga tgttgaaatg ccaagaatga acggaattga atttctaaga cagcttattc
      841 cccagtatcc cataccggtt gttgtagtaa gctcagtgag tgaaagtgtg tttgatgccc
      901 tcaaggcagg agcagtggac tttgttacga agcccgacat gtcaaatccc aagggagtgg
      961 aagctgttat aaacgagctg ataattaaaa tcaaaattgc ttccacagca aaggtcggta
     1021 atgggaaaaa tgagtccctt ttaatgaaat ccaaaatcaa tacatcagac aggagtttaa
     1081 ataaagtaat agcaattggt gcatcaacag gaggtacaga agcgattttt aatataataa
     1141 agtatttccc taaggatatg ccgggaattg ttattgtcca gcatatgccg ccggttttta
     1201 cccggttgta tgcacaaaga cttgacaatt catgccttat ggaggttaag gaagccagta
     1261 acggtgatat ggtttgcccg ggaagggttt tgattgcacc gggagaatac catatgaaac
     1321 tgaaaaaaaa ggatgggaag tattttgtgg agtgtaccaa ggggcagagg gtaaatgggc
     1381 actgtccgtc ggtggatgtg cttttcaact ctgttgcaga gaccgcaggt atgaatgcca
     1441 tcggaataat tctgacgggc atgggaagtg acggggcaaa agggcttttg gctatgagaa
     1501 agaaaggtgc cagaacattg ggacaggacg aaaagtcatc cgttgtttac ggaatgccca
     1561 aggttgcttt tgaaataggt gcggtggaaa aacaggcatc ccttgaagcc atacctcaat
     1621 tggtttattc cttgttgaag aaggattaaa ttaaacggga aaaaattatg gctgcccaaa
     1681 aactcaatac tggtaaagac aattcgaata atggtcttaa aagcaatgga cgcagcggtt
     1741 gaatttatat aatatgaagc atgcaaccga atcgagaata tcgaaagaaa ggggtcagaa
     1801 aatgagaata cttattgcgg aagatgattt tgccagccga aagttcctgt ataagtattt
     1861 gtcttccttc ggtgaatgcg acatttcaat agacggaatt gaagccgtag attcatttat
     1921 catggcactc gaagaaggaa acccttacga tttgatttgt cttgacaata tgatgccaaa
     1981 gcttgatggg accagagcat tgaaggccat aagggatgtt gaaaaacaaa agggaattga
     2041 agaaggagga cgggtcaaaa ttattatgac catggcatta agtaatacat caaaggtttg
     2101 ttcttccttt gatatccgtt gtgaggctta tacggtaaag cccatagatg agaagaagtt
     2161 cgtcgatgtc ttgaaaaggc tcggtttatt ggaaaactgt cataagtgaa cataaaaccg
     2221 gtgggggctc aacgccccca tagcttttat tagtgggatt ttacggagtg aaatgaccaa
     2281 aaagcgatgg aggcgatgcc cccggagctt ttattagtgg gatttcacga agtgaaatta
     2341 ccaaaaagcg atgggggctc gatgcctccg gagcttttat ttaaagattt ttttggttaa
     2401 gcgtttaaag aagccggttt tgtttttttc tctccacatt ccgatggaac ggtcgacgtt
     2461 gtcaaagtgt ttatccaatt tgctttcaag cttcctaacg ttattttcaa agcaagcacc
     2521 cagttcaagc tttgttttgg ttatttcgtt taccacatgc tcttttgttg actccaactc
     2581 aagggaaata ttgttggtta gctgctcgct gaattcctga aaaaacctgc ttatttctgc
     2641 cggcgtgctg tcagccttaa ccgctgttac cggcacaata ttattctggt ctacgactgg
     2701 aggagggcct ccgataatat tttccgactc aaggaccttt tttattgctt ttatctcaag
     2761 accttcatcc ctcatggttt tgatcttata cataatattt gcaagctcag gagtgtaata
     2821 gcgtctgcct cttttgtcct
//
LOCUS       DAOOJC010000319         2836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_27341_length_2836_cov_3.215031, whole genome shotgun sequence.
ACCESSION   DAOOJC010000319 DAOOJC010000000
VERSION     DAOOJC010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27341_length_2836_cov_3.215031"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..857
                     /locus_tag="PLA01_08375"
     CDS             150..857
                     /locus_tag="PLA01_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPD01348.1"
                     /translation="MNSFKLRGFLTEGAIIRTKHCNSTNWVTNVVYGINDDCIEIDIG
                     LEKNYIENIIMVGDTMKCKYSTDEREFTLIGWVTRIRLEDPQSITIKVHDVQQFKNYR
                     NNYRYDIYLCSVIRKKKNDSNGVFAIMVNLSQNGAAFVVKEEIEKQLGIDEEQKDKIF
                     YFEVYISPKSQLCFEGIIRRKSSNSKGFEYGVEIVDIDINNEKNLNEFLDELEKKDKE
                     FYNKRSSFWSKNSKYNK"
     gene            870..1076
                     /locus_tag="PLA01_08380"
     CDS             870..1076
                     /locus_tag="PLA01_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPD01349.1"
                     /translation="MARVIFNEDRCKGCKLCTTVCPKKIVIIVEDRLNQKGFHPAGVN
                     EMDKCIGCAFCATICPDCAIEVNK"
     gene            1110..2168
                     /locus_tag="PLA01_08385"
     CDS             1110..2168
                     /locus_tag="PLA01_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPD01350.1"
                     /translation="MGEKLLMKGNEVIAEAALRAGCRHYFGYPITPQTEVAHYMAKKM
                     PEYGGTFVQAESEVAAINMVYGAASAGARVLTSSSSPGISLKQEGLSYIAGAELPAVV
                     VNIVRCGPGLGGILPAQCDYFQAVKGGGHGDYKMVVLAPSSVQELYELTVEAFNIADR
                     YRIVSMIMGDGILGQMMEAVEFKEKENIEKAEKNWATTGTQMKREHNTVTSIYIQPEV
                     LEKHNQKLQAKFALIQEKETRVESFNCEGADIIVTAFGTVARIVKNVIKMAQKEGIKV
                     GLIRPITLWPFPVSEFEKYADVPKAFLSVELNAGQMVEDVKLAVNGKKPVHFYGRMGG
                     MIPTQKEILDKIKEILNK"
     gene            2202..>2836
                     /locus_tag="PLA01_08390"
     CDS             2202..>2836
                     /locus_tag="PLA01_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPD01351.1"
                     /translation="MDTVFKRPHALTDITMHYCPGCTHGIAHRLIAEVIDELGIEGKT
                     VGVSPVGCAYNNYIYFNCDMVQAAHGRAPAVATGVKRVNPDNFVFTYQGDGDLAAIGT
                     AEIVHAATRGEKITVIFINNAIYGMTSGQMAPTTLIGQVTTTSPFGRKPELHGYPVKV
                     CELLSTLEGAVYVERVSLHDVKNIKNAKNAIKKAFKVQAAGLGFSIVEVLSS"
BASE COUNT          939 a          421 c          711 g          765 t
ORIGIN      
        1 attgtcccaa aagcttaata taccaattgg gtttataagc ggcttctgtg agctaatggg
       61 agaggttcag aatgcagcag gtaccgatat attgtgtatg gacaaactga taaaattacc
      121 atgggattaa tattaatgga ggggttgtag tgaacagttt taagttgagg ggatttctta
      181 ctgagggtgc tataataagg acgaaacatt gcaactcaac aaactgggtg actaacgtag
      241 tttacggtat caatgacgat tgcattgaaa tcgatatagg gcttgagaaa aattatatcg
      301 aaaacataat catggtggga gatacaatga aatgcaagta ttccaccgat gagcgtgagt
      361 ttacgctaat agggtgggtt accagaataa ggctcgaaga tcctcagagt attactataa
      421 aggtacatga tgtgcagcag tttaaaaatt accgcaataa ttacaggtat gatatatatt
      481 tatgttccgt aatcagaaag aaaaagaatg atagcaacgg cgtctttgct atcatggtta
      541 atttaagcca gaacggagca gcctttgttg ttaaggagga aattgaaaag cagctgggga
      601 ttgatgaaga acaaaaagat aaaatctttt attttgaagt ttatatttct ccaaaaagtc
      661 aattgtgttt tgagggaata atcagaagga aaagcagcaa ttcgaagggt tttgaatatg
      721 gtgttgagat tgtggatatt gatattaaca atgaaaaaaa tctcaatgaa tttttagatg
      781 aacttgaaaa aaaggataaa gagttctata ataaacgaag tagcttttgg tcgaagaaca
      841 gcaagtataa taaataatgg gggatgtaaa tggcaagggt tatttttaat gaggacaggt
      901 gtaaaggctg taagctttgt acgacagtat gtccgaaaaa gattgttatt attgttgagg
      961 atagattaaa ccaaaaaggt ttccatccag ccggtgtaaa tgaaatggac aagtgtatcg
     1021 gatgtgcttt ttgtgcaact atatgtccgg attgtgcaat tgaagtaaat aagtaaaata
     1081 tgatcgatgg attaggggga catttatata tgggagaaaa attattaatg aagggtaacg
     1141 aagttatagc agaagctgct ttaagggcag gttgcaggca ttacttcggt tatcccataa
     1201 caccgcaaac agaagtcgca cactacatgg caaagaaaat gcctgagtat ggaggtactt
     1261 ttgttcaggc agaaagcgaa gttgccgcaa taaacatggt ttacggtgcg gccagtgcag
     1321 gagccagggt tttgacttca tcgtccagcc cgggaataag ccttaagcaa gaaggcttgt
     1381 cctatatagc tggtgccgag cttccggctg ttgttgtaaa tattgtaaga tgcggaccgg
     1441 gtttaggagg tatcttacca gctcagtgcg attattttca ggcagttaag ggcggaggac
     1501 acggagacta taagatggta gttcttgctc cctccagtgt tcaggagtta tatgaactga
     1561 cggttgaagc ctttaacata gccgaccgtt atagaattgt gtcaatgata atgggtgacg
     1621 gcatattggg tcaaatgatg gaagccgttg agtttaaaga aaaggaaaac atagaaaagg
     1681 cagagaagaa ttgggcaacc acagggacac agatgaaaag ggaacacaat accgtaacct
     1741 ccatctatat tcagcccgag gttttagaaa agcataacca gaagcttcaa gctaaatttg
     1801 cattgattca ggaaaaagaa acccgtgttg aaagctttaa ctgtgaaggt gcggatataa
     1861 ttgtgacagc tttcgggacg gttgcaagaa tagttaagaa tgttataaag atggcacaga
     1921 aagagggtat taaggttgga ctgataagac ctataaccct gtggcctttc ccggtaagtg
     1981 agtttgaaaa atacgccgat gttccaaaag cattcttatc ggtagaactc aatgcgggac
     2041 agatggttga agatgttaaa ctggccgtca acggcaaaaa acctgtgcat ttttatggca
     2101 gaatgggtgg aatgataccg actcagaagg aaattctgga taagattaaa gaaattttga
     2161 ataaataaga ttgagcattt tattttggaa ggggaagaaa aatggataca gtttttaaaa
     2221 gaccgcatgc tttaaccgat ataaccatgc attactgtcc aggatgtacc catggtatag
     2281 ctcacaggct aattgcagag gtaatagatg agcttggtat tgaaggaaag acagtagggg
     2341 tttcaccggt gggatgtgca tacaataact atatatattt taattgcgat atggtacagg
     2401 cagcccatgg cagggcaccg gcagttgcta ccggtgtaaa aagggtaaat cccgataatt
     2461 ttgtatttac ctatcagggg gacggagatt tggcggcaat aggtacggca gaaattgttc
     2521 atgccgctac aagaggcgag aagataacgg ttatatttat aaataatgct atttacggaa
     2581 tgacatcggg acaaatggca cctactacct tgattggtca ggttaccacc accagtcctt
     2641 ttggaaggaa accggagcta cacgggtatc ctgtcaaggt ttgcgagctt ctatccacac
     2701 tggagggtgc tgtgtatgta gaaagggttt cattgcatga cgtaaagaat atcaagaatg
     2761 cgaaaaatgc tattaagaaa gcctttaagg tacaagctgc agggcttggt ttttcaattg
     2821 ttgaagtttt atcctc
//
LOCUS       DAOOJC010000320         2829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_27435_length_2829_cov_3.598053, whole genome shotgun sequence.
ACCESSION   DAOOJC010000320 DAOOJC010000000
VERSION     DAOOJC010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27435_length_2829_cov_3.598053"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PLA01_08395"
     CDS             complement(<1..1097)
                     /locus_tag="PLA01_08395"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPD01352.1"
                     /translation="MKFDTIEAAIEDIRQGKMIVVVDDEDRENEGDILMAAEKVTPGS
                     INFMATYGRGLICAPITEARSRELNLDLMVHDNTEHMRTSFTVTVDHVSSTTGISAFE
                     RAKTIKELSNPEAKPGDFVRPGHIFPLVAREGGVLKRAGHTEAAVDFARMAGLYPAGA
                     ICEIMNDDGTMARVPQLMDFVKEHGLKIITIADLISYRRNTEKLIRKAAEASLPTEFG
                     EFRIMAYENVMNGEHHVALVKGDVASSEEPVLVRVHSECLTGDAFHSKRCDCGEQLNI
                     AMKKISEENRGVILYMRQEGRGIGLVNKIRAYELQDKGKDTVEANVLLGFAPDLREYG
                     IGAQILCDLGIKKIKLLTNNPKKLIGLGGYG"
     gene            complement(1141..1797)
                     /gene="ribE"
                     /locus_tag="PLA01_08400"
     CDS             complement(1141..1797)
                     /gene="ribE"
                     /locus_tag="PLA01_08400"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPD01353.1"
                     /translation="MFTGIIEEIGIVDGIKSGSMSVKLSIKCRKIMDDVKIGDSISVN
                     GICLTVTSRSFEGFTADVMPETMRRTNLGRLKTKDKVNIERALRLNDRLGGHIVSGHI
                     DGTGVISDKGEEDNAIWLEICAAEDILRYIVQKGSVALDGTSLTVAYVDDKCFRVSLI
                     PHTAGVTTLGTRKIGDIINIECDMLGKYVEKLIKTDYSKERNTKKELTMDFLRENGFV
                     "
     gene            complement(1871..>2829)
                     /gene="ribD"
                     /locus_tag="PLA01_08405"
     CDS             complement(1871..>2829)
                     /gene="ribD"
                     /locus_tag="PLA01_08405"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPD01354.1"
                     /translation="LGKSHAEVDAFDNAAEDVSGSTLYVNLEPCSHYGRTPPCAKAII
                     DANIKKVVVAMVDPNPKVSGRGIKMLRDAGIEVKVGVLENEALKLNEIFINYIVKKRP
                     FVIMKSAVTLDGKIATASGDSKWITGEEARNYVHVIRDRVSAIMVGINTVLKDNPSLT
                     ARRTDKEGRDPIRIIVDSKGILPEDSNVINIDSKAGVILATTCAIPEDKEERLCQRGI
                     KVIKADGTDKRVNLQVLMDELYKLEIDSVLLEGGGTLNSSAISSGIVNKVMYFIAPKI
                     IGGKNAPTSVEGDGADKMNKALMVRDISIRKFGDDILLEGYI"
BASE COUNT          725 a          753 c          439 g          912 t
ORIGIN      
        1 aatccgtaac cgccaagacc tatcagtttc ttgggattat tggtaagcag ctttattttt
       61 ttaataccga gatcgcataa aatctgtgcc ccaattccat attcccttaa atccggtgcg
      121 aaccccaaaa gtacattggc ctcaacagta tcctttcctt tatcctgaag ctcatacgcc
      181 cgtatcttgt ttacaagtcc aatgccccgg ccttcctgcc tcatataaag gattacccct
      241 ctattctctt cacttatttt cttcatggca atattcagtt gttctccaca atcgcagcgt
      301 ttcgaatgaa aagcatcccc cgtaagacac tccgaatgaa ccctcaccag tacaggctcc
      361 tcggaacttg ctacatctcc ctttaccaat gcgacatggt gttcaccgtt cataacattt
      421 tcatatgcca ttatcctgaa ttccccgaat tcggtaggaa gacttgcctc tgcagccttt
      481 cttataagct tttcagtatt tctcctataa cttatcaggt ctgcaatggt tatgatctta
      541 agcccgtgct ctttcacaaa atccatgagc tgcggaactc ttgccatggt tccgtcatca
      601 ttcattatct cacatatagc tccggcagga tacagacctg ccattcttgc aaagtcaact
      661 gctgcctccg tatgccctgc cctttttaac actccgccct cccgtgctac caaaggaaaa
      721 atatgccccg gccttacaaa atctccgggt tttgcttcgg gatttgaaag ctcctttatt
      781 gtttttgccc tttcaaaggc agaaatgcct gtggtggaag aaacatggtc caccgttaca
      841 gtaaaggagg ttctcatatg ctcagtgtta tcatgaacca tcaagtcaag gttcaattcc
      901 cttgaccttg cctctgttat aggtgcacat attagtcctc ttccgtaggt tgccatgaaa
      961 ttaatgctcc cgggtgtaac cttttcggca gccataagaa tgtctccttc attttccctg
     1021 tcctcgtcat caaccacaac tatcatttta ccctgccgta tatcctcaat tgccgcttca
     1081 attgtatcga atttcataaa actgcctccc taataaccgt tattttatat atcatacgat
     1141 ttatacaaaa ccgttttctc tcaaaaagtc catggtcagt tctttttttg tgttcctttc
     1201 cttagaataa tcagtcttta tcagtttttc cacgtacttt cccagcatat cgcattcaat
     1261 gttgattata tcaccgattt ttctcgttcc aagggttgta accccggcag tatgaggtat
     1321 cagtgaaacc ctgaagcact tatcgtcaac atatgcaaca gtaagactcg ttccgtccaa
     1381 tgccaccgaa cccttttgaa caatataccg caatatatcc tctgctgcac aaatctcaag
     1441 ccaaattgcg ttatcctctt cgcccttgtc tgatataact ccggtaccat cgatatggcc
     1501 gcttacaata tgccctccca gtctgtcgtt aagtcttaag gctctttcaa tattaacctt
     1561 gtctttggtc ttaagccttc cgaggtttgt ccttctcatg gtctcaggca tgacatctgc
     1621 cgtaaaccct tcaaaagaac gggatgttac ggtaagacat attccgttga cagaaatact
     1681 atccccaatc tttacatcat ccataatctt tcggcactta atacttagtt taaccgacat
     1741 actgccggac tttatcccat caactatacc gatttcctca ataatacctg taaacatttt
     1801 ctactcctgt aacacactta tttgaattct gcgatttttc attccataag gcaacgttca
     1861 taatacactg ttatatatac ccttccaaaa gtatatcatc tccgaatttt ctaatggaaa
     1921 tgtcccttac cataagagcc ttattcattt tatccgcacc atccccttca acagaagtcg
     1981 gtgcattctt tcctccgata atttttggag caataaaata cattacttta ttgactattc
     2041 ctgagcttat tgccgaactg ttcagagtac ctccgccctc taacaagaca ctgtcaattt
     2101 caagcttgta aagctcatcc ataagaacct gaaggtttac tcttttatcg gtgccatctg
     2161 ctttaataac ctttatgcct ctttgacaaa gcctttcttc tttgtcttca ggaattgcac
     2221 aagtagttgc aagtataacc ccagcctttg aatcaatgtt tatgacattg ctgtcttccg
     2281 gcaatatacc cttgctgtcc acaattatcc taataggatc ccttccttct ttgtcagttc
     2341 ttcttgcggt caaagaagga ttatccttta aaacggtatt aattcccacc atgatggctg
     2401 aaaccctgtc ccttattaca tggacataat tgcgggcttc ctctccggtt atccacttgg
     2461 aatctcccga agccgtagca attttgccgt ctaaagtcac tgcggatttc ataattacaa
     2521 agggtctctt tttaacaata taatttatga atatctcatt aagctttaaa gcctcattct
     2581 cgagaacacc aaccttgact tcaatccctg catcccgaag catttttata cctctgccgg
     2641 aaaccttcgg gtttgggtca accatggcta caacaacctt ctttatgttt gcatcaataa
     2701 tcgccttggc acagggagga gtccttccat aatgggaaca tggctctaaa ttgacgtaca
     2761 gggtacttcc cgaaacatcc tctgccgcat tatcgaatgc atcaacctcc gcatggcttt
     2821 ttcccaatg
//
LOCUS       DAOOJC010000321         2825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_27499_length_2825_cov_3.155957, whole genome shotgun sequence.
ACCESSION   DAOOJC010000321 DAOOJC010000000
VERSION     DAOOJC010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27499_length_2825_cov_3.155957"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            428..1648
                     /locus_tag="PLA01_08410"
     CDS             428..1648
                     /locus_tag="PLA01_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD01355.1"
                     /translation="MYIKRHIEFAVSERAREKGAVIVTGARQVGKTTLIENIKPDISR
                     VTFDDITVRHRVKKDPAAFLQLNPPPLFIDEVQYVPEIFHYIKISLDRSRNKGDFYLT
                     GSQSFELMKNVSESLAGRAGILNLMGLSLREMRNENWNEPFIPTTDYLMRRKAAKIDL
                     NINEVWKIIHRGCMPELFKEQSYNWQNFYSDYIKTYIERDVRNLTQVADESDFMKFMT
                     VCAAMTGSLLNIASLAKEVGISQPTAKRWLSILKTSNIVYLLKPYSNNAIKRAIRTPK
                     LYFLDTGLAAYLTRWLTPETLSTGAMNGHFFETFVVSELIKSYANRGMDEYFYFLRDS
                     NGREIDLLIFQDNTLFPIEIKKHTNPSYRDIASFDMLKSVKNITIGEGGVICPSQDLL
                     PLKDNHKIIPLWAV"
     gene            1741..2304
                     /locus_tag="PLA01_08415"
     CDS             1741..2304
                     /locus_tag="PLA01_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPD01356.1"
                     /translation="MKILLELYWVFFKVGMFAFGGGYTMLPLLQKEIVEKKKWLTSEE
                     IIDYYTLSQCSPGIIAVNVSTYIGFKKKRYLGGIVSALGVATPSIIIITVIAAVITNF
                     LHIKEVSYAFAGIRVAVAALVIVTIVDMYKKSVVDWLCIILYITSFLLSVLTSISPVF
                     IVLGAGITGLVLGTRMRERKGDKNDIS"
     gene            2291..>2825
                     /locus_tag="PLA01_08420"
     CDS             2291..>2825
                     /locus_tag="PLA01_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPD01357.1"
                     /translation="MIYLELFWEFFKTGLFSVGGGLATIPFLYDIGRRYDWLDTSIIP
                     DMIAISESTPGPIGINMATYTGYTAAGILGALIASIGVILPSVIIVLLISRFLVSFSQ
                     DKRVLNTMYGLRPASLGLISAAAFTLLNVSVITYDAFVESGNILDIINIKALVLFVCL
                     LVIRLFFRKIHPILFIVF"
BASE COUNT          847 a          528 c          564 g          886 t
ORIGIN      
        1 tctactatta ccgtatagcc aaaggtatat taaaaatact ctcttatgca cgtatgggtg
       61 taaaagggtg gttcaaaatt aagcgtcaaa agtacagaca attagccttc agttttttat
      121 cctgagctat acaaaattga atactcatga aaagttccga catagtggga actgattttc
      181 atacacaaaa ctccggtttt agagactttt atatgcaatt ttactaatca ttcctctata
      241 gtcttaaagc taacctcaaa aaccggtcaa ttatccggtt tcgagtgcat tttacggaaa
      301 ctcaagttat tcttctaccg gataaatccg tttgtttttc cagccttgct atatgctatt
      361 tgtttttgca aaaacatatg atataataac atatgatgtt agaatatcat aaatatgtga
      421 ggtagttatg tatataaaac gacacataga atttgctgtt tcggagcggg caagggaaaa
      481 gggagctgtc atagttaccg gagcacgaca ggtcggcaag actacattaa ttgaaaatat
      541 taaacccgat atatcgcgag ttacttttga tgatataacc gttcggcatc gtgtaaagaa
      601 ggatcctgct gcatttttgc agctaaatcc gccacctctt tttattgatg aagtacaata
      661 tgtcccggaa attttccatt acataaaaat ttctttagac cgaagtcgta ataaaggaga
      721 tttttatctt accggttcac aaagttttga acttatgaag aatgtcagtg agagcttagc
      781 cggtcgtgcg ggaatattaa atctgatggg gctttcgtta cgggaaatga gaaatgaaaa
      841 ctggaatgaa cccttcatcc ctactaccga ctatctaatg agacgtaagg ctgcaaaaat
      901 agacttgaat ataaatgaag tttggaaaat tattcatcgc ggctgtatgc ccgagttatt
      961 taaggaacaa agttataact ggcaaaactt ctattccgac tatattaaga cgtatataga
     1021 aagagatgtt cgaaatctca cacaggtagc tgatgaatcg gattttatga aatttatgac
     1081 tgtttgtgcc gccatgaccg gtagcctgtt aaatatagct tcccttgcaa aagaagtggg
     1141 aatcagtcag ccgacagcga agcgttggct ttctatattg aaaacgagca atattgttta
     1201 tttattaaaa ccttactcaa acaatgcaat caaacgagca ataagaacac ccaaacttta
     1261 ctttttggat acaggccttg cagcatattt gacccgctgg cttacacctg agactctttc
     1321 tacaggtgca atgaacggac atttctttga gacttttgtt gtttcggagt tgattaaaag
     1381 ttacgcaaac agaggtatgg acgagtattt ctattttctt cgtgacagta acggtagaga
     1441 aattgatctt cttatcttcc aggataatac actattccct attgaaatca agaaacatac
     1501 aaatccttct tatagggata tagcaagttt tgacatgctg aaatccgtaa aaaatataac
     1561 tataggggag ggcggtgtaa tatgcccttc acaggatttg ctaccgctta aggataatca
     1621 caaaatcata cctctgtggg ctgtataatt taatagaatt taacaatctt attacccgtg
     1681 aaacatgttt ctctcttaat atttaaccct aaacaggcta taataaaaag aggaggcttt
     1741 atgaagattc ttttagaact gtattgggtt tttttcaaag tcgggatgtt cgctttcggc
     1801 ggcggctata cgatgctgcc tcttcttcaa aaagagattg tggaaaaaaa gaagtggctc
     1861 acctccgagg aaatcattga ttactatacc cttagccagt gctcgccggg cattatcgcc
     1921 gtcaatgttt ccacatatat aggtttcaaa aagaagcgtt atctcggagg catagtcagc
     1981 gccttgggtg ttgcaactcc ttcaattata attataacgg taatagcggc ggttataaca
     2041 aattttctgc atataaaaga agtctcttat gcctttgcgg gaatcagggt agccgttgcg
     2101 gcacttgtta tagttacaat agtggacatg tataaaaaat cggttgtgga ctggctttgc
     2161 atcatacttt acatcacttc cttccttttg tcggtactta cttcgatatc acccgtattc
     2221 atagtactgg gcgccggcat taccggactg gttctgggaa caagaatgcg ggaaagaaaa
     2281 ggagataaga atgatatatc ttgagctgtt ctgggaattt ttcaagaccg gccttttctc
     2341 cgtaggagga ggtcttgcaa caataccttt cctttatgat atcggaagga gatatgactg
     2401 gctggacaca tccataatcc ccgatatgat agctatatcc gaatccactc cgggtcccat
     2461 aggaataaac atggcaactt atacgggcta caccgctgcc ggcattttag gtgcattgat
     2521 tgcttccata ggggtaattc ttccttccgt cattattgtc cttctgattt cgaggttttt
     2581 ggtttctttc agtcaggaca aaagagtcct caataccatg tacggattac gacccgcttc
     2641 attgggactt atatcggcgg cagcttttac gcttctcaat gtttcggtca taacatatga
     2701 tgcttttgtt gaatccggga atattcttga tataatcaat ataaaggccc tggtattgtt
     2761 tgtttgttta ctggtaatta gactgttttt cagaaaaatc caccctatac tgtttatagt
     2821 ctttg
//
LOCUS       DAOOJC010000322         2818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_27590_length_2818_cov_4.169019, whole genome shotgun sequence.
ACCESSION   DAOOJC010000322 DAOOJC010000000
VERSION     DAOOJC010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27590_length_2818_cov_4.169019"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1744)
                     /locus_tag="PLA01_08425"
     CDS             complement(200..1744)
                     /locus_tag="PLA01_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPD01358.1"
                     /translation="MKSERSGRCFLLLLVLFLLIKGLFPIFNVSEADAKSDGTIVLKI
                     GNWEEYIDQGDWDEDEAIELESETITSENGVISDFQDWYYDTYGKRVEVEYSTFGTNE
                     DLYSKIILGETYDLVCPSEYMFSKLLKEGRLQPLSESFFDTSDEYNYYINGVSPYIKH
                     IFNINGIDGKSWNEYAAGYMWGTIGIVYNPEEVSEEDASSWSILADPKYNKRVTIKDS
                     VRESYFPALAILHKDLLLSEEFINSEDYSESLAGLLNDTRPQTIEKAEDLLKIIKENV
                     YSFEVDSGKADMVTGKIVANLQWSGDAVYTMEQAEEDEYYLNYAVPKECTNLWLDGWV
                     MLKSGINGDMEKQKAAEAFINFISRPDNVIRNMYYIGYTSVISGGDSPLIYEYANYRF
                     EAEDDEATVEYPLGYFFSGVDEDEDFVINTTEDQAKRLLFAQYPPKDVIDRSAVMGYL
                     PDDVNAQLNQMWINVRCFNFNQVSKAVWLRALFIICFILILIIIWVNRYRIFRKPIRP
                     GFYRVD"
     gene            complement(1734..2546)
                     /locus_tag="PLA01_08430"
     CDS             complement(1734..2546)
                     /locus_tag="PLA01_08430"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD01359.1"
                     /translation="MIKKILSSSWIGIVLLFMYSPILILSVYSFLDTPTIGASGNFSL
                     QNYITLFTSEELMEMIIGTLTLALQCAVISTILGTLGAIGAFYSKKGMKGFVNTSNQI
                     PVVNADVVTGFSICVLLIVVFGVSKDTFIPLIIGHVVMATPFVYLSVMPKLKQMDHNL
                     YEAALDLGANARQALFKITIPQIMPGIVSGFLLAVTLSLDDYFVTTYTKPATFNTIST
                     YVVNATKGSNTEMKTALWALSTLIFAIILFTVLISNINTNRKAGEENEKNEI"
     gene            complement(2543..>2818)
                     /locus_tag="PLA01_08435"
     CDS             complement(2543..>2818)
                     /locus_tag="PLA01_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPD01360.1"
                     /translation="EVFTRVTLPLSLPGIVSGVTMVFLPAMTNYVILDMLYNNTFIMG
                     SLIGSYFNAYDWHSGSMISIVLLMIIFIITWATKGFTEKEPEKEAIL"
BASE COUNT          887 a          656 c          442 g          833 t
ORIGIN      
        1 aatacccgtg gctagacttt cactagctag attagtgcca tgcctggcac acaacaaaat
       61 gcctccataa acgtattgat tatgcaggca ttttatcttt taaaaccaat tatttatacc
      121 tatcgaaatt atcccactaa caaatgctcc tgacatcaag cccccatcgt tttttgtcat
      181 ttcactttat ataatcccgc taatcaaccc tgtaaaaccc gggtcttata ggttttctga
      241 aaattctata gcggttaacc caaataataa tcagaatcaa aataaagcaa atgataaaaa
      301 gtgctctaag ccaaactgcc ttagaaactt gattaaaatt aaagcatctt acattaatcc
      361 acatctggtt taactgtgca tttacatcat ccggaaggta ccccattaca gcactccggt
      421 caataacatc ctttggcgga tattgagcaa ataaaagccg ttttgcctga tcctcagtag
      481 tattaatcac aaaatcttca tcctcatcaa cgccgctgaa gaaatagcct aagggatatt
      541 ctaccgtagc ttcatcatct tcagcctcaa atctatagtt ggcatattca tatatcaaag
      601 gactgtcccc gcctgaaatg acagaggtat aaccgatgta atacatattc ctgattacgt
      661 tatccggtct ggaaataaaa ttaataaaag cttctgctgc tttttgcttc tccatatccc
      721 cgtttattcc ggacttgagc attacccatc catcaagcca tagattggtg cactccttag
      781 gaacagcata attaaggtaa tattcgtcct cttcagcctg ttccattgtg tataccgcat
      841 ctccggacca ctgcaaattg gcaacgattt tacctgtaac catatccgct ttaccgctgt
      901 caacttcaaa agaatataca ttctccttga ttatcttcag caaatcctcg gctttttcaa
      961 ttgtttgcgg tctggtatca ttcaaaagcc ccgccagact ttccgagtaa tcctcgctgt
     1021 taataaactc ctcgctgagc agcaaatcct tgtgtaaaat agccaacgca gggaaatagg
     1081 attctctgac actatccttt atagttactc ttttattata tttgggatcg gcaagaattg
     1141 accagcttga agcatcttcc tcacttacct cttccggatt atatacaatt ccaatagtgc
     1201 cccacatgta acctgctgca tattcattcc aggactttcc gtcaatcccg tttatattga
     1261 aaatatgttt aatatacggt gatactccgt ttatataata attatactcg tcggatgtat
     1321 caaaaaaact ctccgacaac ggctgaagcc ggccttcttt caacagcttc gaaaacatat
     1381 attcagacgg acaaaccagg tcataggtct ctccgagaat gattttgctg tacagatcct
     1441 cattggttcc aaaggttgag tattcaacct ccacacgttt accgtaagtg tcataatacc
     1501 aatcctggaa atccgaaatt acgccgtttt cacttgtaat tgtctcgctt tcaagctcta
     1561 tggcctcgtc ctcatcccaa tcgccttgat ctatatactc ttcccaattg ccgattttta
     1621 aaacaattgt accatcgctc tttgcatcag cttccgacac attaaatatt gggaacaatc
     1681 cctttatcag aagaaacaat accaataaaa gcaaaaaaca tcttccgcta cgctcagatt
     1741 tcattttttt cattctcctc accagccttt ctattggtat taatgttgct aataagcact
     1801 gtaaaaagaa tgattgcaaa aatcaacgtc gataaagccc aaagtgcggt tttcatctct
     1861 gtattcgagc cttttgtcgc attcaccacg taggtgctta tggtattaaa tgtcgcgggt
     1921 ttagtatacg tagtaacaaa ataatcgtct aaagaaaggg ttactgccaa taaaaagcct
     1981 gaaacaatac ccggcataat ctgcggaatt gtgattttga ataatgcctg ccttgcattg
     2041 gcacctaaat caagggccgc ttcataaaga ttatgatcca tttgttttag tttcggcata
     2101 acggatagat agacaaaagg agtcgccata acaacatgcc ctatgattaa gggtataaag
     2161 gtatctttac tcacgccaaa cacgacgatt agcaatacgc agatggaaaa tcccgttaca
     2221 acatcagcat taacaacagg aatctgattg gacgtattca caaacccctt cattcccttt
     2281 tttgaataaa aagcaccaat ggcccccagt gttcctaaaa ttgtagatat aacagcacat
     2341 tgcaaagcca aagtcaaggt tccaatgatc atctccatta gctcctcgga agtaaagaga
     2401 gtaatatagt tttgtaagga aaaattaccc gatgccccaa ttgtcggagt gtccaaaaag
     2461 ctataaactg acagaatcag aatcggagaa tacataaaca ataatacaat tccaatccat
     2521 gaagaactca atatcttctt aatcatagaa ttgcctcctt ttccggttcc ttctcagtaa
     2581 acccttttgt tgcccatgtt atgataaata taatcatcaa cagcacaata gatatcatgg
     2641 aaccgctgtg ccaatcataa gcattgaaat aactgccgat caaactaccc attataaatg
     2701 tattattata aagcatgtca aggattacat agtttgtcat tgccggtaag aacaccatcg
     2761 taacaccgct tacaattcca ggcaatgaaa gaggcaatgt aactctggtg aatacttc
//
LOCUS       DAOOJC010000323         2808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_27728_length_2808_cov_3.415547, whole genome shotgun sequence.
ACCESSION   DAOOJC010000323 DAOOJC010000000
VERSION     DAOOJC010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27728_length_2808_cov_3.415547"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /gene="der"
                     /locus_tag="PLA01_08440"
     CDS             complement(<1..1117)
                     /gene="der"
                     /locus_tag="PLA01_08440"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPD01361.1"
                     /translation="MPKPVVAVVGRPNVGKSTFFNYLAGKRISIVEDTPGVTRDRIYT
                     EVEWRSRKFTLIDTGGIEPYAEDKIMQQMKRQAEIAIDMADVIIFMVDMKDGITASDR
                     EVSTMLRKTKKPVILTVNKVDKVGEPPSDVYEFYNLGLGELMTISSIHGLGIGDLLDE
                     VYKYFPEDDQEDYEEDVIRVAVVGKPNVGKSSLVNRIIGEERVIVSDIPGTTRDAIDT
                     YVENEYGKFVFIDTAGIRKQSRINESIEKYSTIRSWTAIERADVCLILIDAQEGVTEQ
                     DTKIAGYAHEQGKASVIVVNKWDLVEKETGTLEEYRKTVNEKLGFMLYAPVLFISAKT
                     GQRVDRTFELIKKVAEQAALRISTGVLNDILNQATAMV"
     gene            complement(1180..2502)
                     /locus_tag="PLA01_08445"
     CDS             complement(1180..2502)
                     /locus_tag="PLA01_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HPD01362.1"
                     /translation="MKYRPIAITNVISDSIAQEAGLKNGDLILSINGEKISDIFDYRF
                     LIANETLLLEIQKKDGEIWEVEIEKDEYEDLGIEFENLMLDDAKSCSNKCIFCFIDQL
                     PKGMRKTLYFKDDDSRLSFFMGNYVTLTNMDFNDIDRIIKYKMSPINVSVHTTNPELR
                     VYMLNNKFAGDIMEKIKRLVEGGITVNCQIVLCRDINDGVELDRSINDLASLYPGMNS
                     ISVVPVGLTKYREDLTVLKPYDKESSKKVIEQVEAWQKKLLLEKGSRVVYLADELYVM
                     AELQIPDYSVYEDFPQIENGVGLIAMLRQEFDEYLALLDFKPEKRRKVSIATGVSSYK
                     YIKELADVLENKYKDLNVNVYKIKNEFFGENVTVTGLITGQDLVKQLFDKDLGEELLI
                     SVSMLRSGEEVFLDDYTVDMLEKELGIRVTVVENCGNDFVEKILGIPL"
     gene            complement(2598..2798)
                     /gene="rpmF"
                     /locus_tag="PLA01_08450"
     CDS             complement(2598..2798)
                     /gene="rpmF"
                     /locus_tag="PLA01_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPD01363.1"
                     /translation="MANPKRKWSKARTGKRRSQWKLSAPNLVECPRCHSLKLLHRVCK
                     ECGHYTVVRKGERKAIEVLSVE"
BASE COUNT          770 a          720 c          412 g          906 t
ORIGIN      
        1 gaaccattgc cgtagcctgg ttaagaatgt cattcagtac cccggtggaa atccttaatg
       61 ctgcttgttc agccactttc tttatcaatt cgaaagttct gtccaccctc tgcccggttt
      121 ttgccgaaat aaaaagcaca ggtgcgtaca gcataaagcc cagtttctca ttaaccgttt
      181 tccggtactc ttccaaagtg cctgtctctt tctcgacaag atcccactta ttgaccacta
      241 ttaccgatgc tttcccctgt tcatgagcat atcctgctat cttggtatcc tgctcggtaa
      301 ccccttcctg ggcatcaatc aatatgaggc aaacatccgc cctttcaatt gcagtccagg
      361 acctaatcgt actgtacttt tctatgcttt cattaatcct gctctgcttt cttattccgg
      421 cggtatcaat aaaaacaaat ttaccgtact cgttttcaac ataggtatca atggcatccc
      481 ttgttgtccc cggaatatca ctgacaataa ccctttcctc tccgattatc ctgttgacga
      541 gggatgattt acccacattg ggttttccta ctacagcaac tcgtattaca tcctcttcat
      601 aatcttcctg atcatcctct gggaaatatt tataaacttc gtccaataaa tctccgattc
      661 ccagaccgtg aatcgaagaa atagtcatta gttcgcccaa tccaaggtta tagaactcat
      721 atacatccga tggaggttct cccactttgt ctactttatt gacagtgaga ataacaggtt
      781 ttttggtttt tctaagcatt gtactcactt ccctgtcgga agcagttatt ccatccttca
      841 tgtcaaccat aaaaatgata acgtctgcca tatcaatggc aatttcagcc tgtcttttca
      901 tctgctgcat tattttgtcc tcagcataag gctctatacc accggtgtct attagagtga
      961 atttcctgct tctccactcc acttcggtat atatcctgtc acgggttaca cccggtgtat
     1021 cttcaacaat agaaatcctc ttcccggcca gataattaaa aaaagttgat ttaccaacat
     1081 ttggtcttcc taccaccgca actacaggtt ttggcaattt atctccctcc aaatcttata
     1141 atacagagat aaataaagca gaagccattt atcgccctgt tataaaggga ttcccagtat
     1201 cttttcaaca aaatcattac cgcagttttc tacaactgtc acccttattc caagttcctt
     1261 ctcaagcata tcaacggtat aatcatcaag aaaaacctct tctccggatc ttaacatgct
     1321 tacggatatt aaaagctctt cacccaagtc tttgtcaaaa agctgtttaa ccaaatcctg
     1381 ccctgtaata agtccggtta cagttacatt ttctccaaaa aactcattct ttattttata
     1441 aacattaaca ttcaaatcct tatatttatt ttcaagaaca tccgccagtt cctttatata
     1501 tttataagat gaaacgccgg tcgctattga aactttcctt ctcttttccg gtttgaaatc
     1561 caacagggcg agatattcat caaattcctg ccttaacatt gcaataagtc cgacaccgtt
     1621 ttctatttgg ggaaaatcct cgtacacaga gtaatccggt atctgaagct ctgccatgac
     1681 ataaagctca tccgccagat atacaactct agaccccttc tccaaaagaa gctttttctg
     1741 ccaggcttca acctgttcta taaccttctt ggaagactcc ttgtcatagg gtttcaaaac
     1801 ggtcaaatcc tcacgatact ttgtgagccc cacgggaaca acggaaatac tgttcatacc
     1861 tggatagagt gatgcaaggt catttatcga tctatccaac tcgaccccat catttatatc
     1921 cctgcacagc actatttgac aattaaccgt tattccgcct tccacaagcc tttttatctt
     1981 ttccattatg tccccggcaa acttgttgtt cagcatataa accctcagtt caggattggt
     2041 ggtatgcacc gaaacattaa tcggggacat tttgtatttt ataatcctat ctatatcatt
     2101 aaaatccata tttgtcaaag tcacatagtt gcccataaaa aaagacagcc tggaatcatc
     2161 gtccttaaag taaagagttt ttctcattcc tttgggcaac tggtctataa agcaaaaaat
     2221 acacttattg ctgcagcttt tggcatcatc aagcatcaaa ttctcaaatt caatgcccag
     2281 gtcctcatac tcatccttct caatctctac ttcccagatt tctccgtcct ttttctgaat
     2341 ctccagcaaa agggtttcat tggcaatcag aaatctgtaa tcgaatatgt cacttatttt
     2401 ttctccgttt atggacagta tcaggtctcc attcttaaga cctgcctctt gtgcaatact
     2461 atccgaaata acattggtta ttgctattgg cctatacttc aacccaagtc ctccctattt
     2521 ttattaaaaa atagacacct tcctcttcat aaaccctatc taagcaaaaa gcagcaggtt
     2581 taactgctgc ttttactcta ttctacgctt agaacctcaa ttgctttacg ttcaccttta
     2641 cgaaccaccg tataatgccc acactcttta caaactctat gaagcagctt taaggagtga
     2701 caacgcggac actcgaccaa atttggtgct gacagcttcc actgagatct tctttttccc
     2761 gttcttgcct tagaccattt acgctttgga tttgccatga attacacc
//
LOCUS       DAOOJC010000324         2784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_28054_length_2784_cov_2.868450, whole genome shotgun sequence.
ACCESSION   DAOOJC010000324 DAOOJC010000000
VERSION     DAOOJC010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28054_length_2784_cov_2.868450"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>935)
                     /locus_tag="PLA01_08455"
     CDS             complement(<1..>935)
                     /locus_tag="PLA01_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HPD01364.1"
                     /translation="NCPMVTSYPEVIKNNMDSLREKGIVYLNPFLPVDNKKRLTERLY
                     EVFRVYNISRDEIKQAVEKAWLEDENVKQDIRKKGEETLEYLEKSGKKGIVLAGRPYH
                     IDPEINHGIPNIITGFGMAVLTEDAIAHLGKIQHPLRVIDQWTYHSRLYRAASFVKEK
                     SNLELVQLNSFGCGLDAVTTDQVEEILASSNKIYTVLKIDEIDNLGAAKIRIRSLKAA
                     MEERDKNGFKPVKGDSSCKKVLFTKEMKEKHTILAPQMSPIHFQFLEEAFNVSGYNLK
                     VLPYVDRKAVDEGLKYVNNDACYPSIIVVGQLMEAL"
     assembly_gap    936..945
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<946..>2784)
                     /locus_tag="PLA01_08460"
     CDS             complement(<946..>2784)
                     /locus_tag="PLA01_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPD01365.1"
                     /translation="NEMAKNYRVIYSIAARCGVFAKTDIQPLINEGASREDIAASIFQ
                     SVVNQTISGLACGKPIRGNVAFLGGPLQFLSELRVRFVETLKLEEHQVISPDNAELYV
                     ALGAALASKDLKGIPFRSLAGRLSVLNEVTVHEVERFAPLFRDEKELEDFRIRHQNSA
                     VSKRDIKGYAGKCYLGIDAGSTTTKAVLIDETGAILYSHYGSNNGSPLKSTVKILREI
                     YGLLSKDAKIVNSTVTGYGEGLIKAALAVDIGEIETIAHYKAADAILPGVEFILDIGG
                     QDMKCLKVKNGVIDSIMLNEACSSGCGSFIETFAHSLNMSVGDFAKEALLAKSPVDLG
                     SRCTVFMNSRVKQAQKEGASVGDISAGLSYSVIKNALQKVIKMRDPKEMGQKVIVQGG
                     TFYNDAVLRSFELIAGKNAVRPDIAGLMGAYGAALIAKERYTQGYESTLVKSEELDSF
                     SFDISMRRCGLCGNNCLLTINTFSGGKEFVSGNRCERGAGVEKKRDDIPDLFDYKYKR
                     MFSYKPLDKNEAIRGTVGIPRVLNLYENYPFWFTFFTELKFRVELSPRSSKKIYELGM
                     ETIPSESACYPAKIVHGHITSLINKNVDIIFYPCLPYEKIEYRDCN"
BASE COUNT          722 a          720 c          479 g          853 t
ORIGIN      
        1 agggcttcca tcagttgtcc cactacaatt attgaaggat agcaggcatc attgtttaca
       61 tatttcagcc cttcatcaac cgcttttctg tcgacataag gcaatacctt caggttgtag
      121 ccggaaacgt tgaatgcctc ctccagaaac tgaaaatgta taggcgacat ctgcggtgcc
      181 aaaatggtgt gcttttcttt catttccttt gtaaaaagca cttttttaca gctggagtca
      241 ccctttacgg gtttgaaccc gtttttgtcc ctttcttcca tcgcagcttt caaggacctt
      301 atacgaatct ttgccgctcc caggttatca atctcatcaa tttttaagac tgtataaatc
      361 ttgttactgc ttgccagtat ttcttcaact tgatcggtgg taacagcgtc cagtccgcaa
      421 ccgaaggaat taagctgaac cagttcaaga ttgctttttt ccttcacaaa gcttgcagcc
      481 ctatacagcc tggaatgata cgtccattgg tcaataaccc tcagtggatg ctgaattttc
      541 cccaaatgtg ctattgcgtc ctccgtaagg acagccattc caaacccggt tataatattg
      601 ggtataccgt ggtttatttc aggatctatg tgataaggac gtcctgcgag aacaatacct
      661 tttttcccgc tcttttcaag gtattccaac gtctcttctc ccttttttct aatatcctgt
      721 tttacatttt catcctccaa ccaagccttt tcaacagcct gttttatctc atcacggctt
      781 atattgtata ctctgaatac ttcatacagc ctctctgtaa gtcttttttt attgtcaaca
      841 ggcagaaacg gattcagata tactattccc ttttccctta aagaatccat attgttctta
      901 atcacttccg gataggaagt caccatggga cagttnnnnn nnnnntgttg caatctctgt
      961 actctatttt ttcatagggc agacacgggt aaaatattat gtctacattc ttattgatga
     1021 gactcgttat atgcccgtga acaatcttag cagggtaaca ggcagactcc gaaggtattg
     1081 tctccattcc cagctcgtat atcttcttcg aagacctggg agaaagctcc accctgaact
     1141 tcaactctgt aaaaaatgta aaccagaagg ggtaattttc atataagtta agcaccctcg
     1201 gtatacccac cgtccccctt atcgcttcgt ttttatccaa gggcttgtat gaaaacatcc
     1261 ttttgtattt ataatcgaaa agatcgggaa tatcatcacg ctttttctct actccggctc
     1321 ctctctcaca ccggttcccg gatacgaact ccttgcctcc cgaaaatgta tttatcgtaa
     1381 gcaagcagtt attaccgcaa agcccgcatc ttctcatcga tatgtcaaaa ctgaagctgt
     1441 caagctcctc cgactttacc agagtacttt catatccttg ggtataccgt tctttggcaa
     1501 taagagctgc tccataggct cccataagac ctgctatatc cggtcttact gcattcttgc
     1561 cggcaataag ttcgaagctt ctgagcacag cgtcattata aaaggtaccg ccctgaacaa
     1621 ttaccttttg tcccatctcc ttcgggtccc tcatttttat tactttttga agtgcgtttt
     1681 ttataaccga ataggaaaga cccgccgata tgtcacccac cgacgccccc tccttttgtg
     1741 cctgcttaac ccttgaattc ataaaaacag tacaccttga ccccaaatcg acaggggatt
     1801 tggctaaaag tgcctctttt gcaaaatccc ctacgctcat attcaatgaa tgggcaaagg
     1861 tttcaatgaa ggaaccgcat ccggaagaac aagcctcatt cagcataata ctgtcgataa
     1921 caccgttctt taccttcaaa catttcatgt cctgtccgcc aatatcgagt atgaattcaa
     1981 ccccaggcaa tatggcatct gctgccttgt agtgtgctat tgtttcaatc tcgcctatat
     2041 caaccgccag ggctgccttt atcagtccct ctccatagcc cgtaaccgta gaattaacaa
     2101 tcttcgcatc tttcgaaagc aaaccataga tttccctgag gatttttacc gtcgacttca
     2161 aagggcttcc gttattactc ccgtaatggg aatacaaaat ggctcccgtt tcatctatca
     2221 gtacggcttt agtagttgtt gagcctgcat ctatgccaag atagcatttc cctgcataac
     2281 ctttaatgtc acgttttgaa acggcactgt tttgatgcct gatacgaaaa tcttcaagct
     2341 ccttttcatc tctgaacaac ggtgcaaacc tttccacctc atgaaccgta acttcattta
     2401 ataccgaaag ccttcctgcc agggacctga aaggtatccc tttcaaatct ttagaagcca
     2461 aggcagcacc taaagcaaca tacagctccg cattgtcggg agaaatcacc tgatgttctt
     2521 caagcttcag ggtttcaaca aatcttaccc taagctccga caagaactgt aaaggtcctc
     2581 ccaagaaagc aacatttccc cttatgggct ttccacatgc caatccgctt atggtctggt
     2641 ttaccaccga ttgaaatatt gatgcggcta tatcctctct tgacgcccct tcattaataa
     2701 ggggctgaat atcggttttg gcaaacaccc cacatctggc agcaatagaa tagataaccc
     2761 tgtaattctt tgccatctca ttca
//
LOCUS       DAOOJC010000325         2765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_28345_length_2765_cov_3.240959, whole genome shotgun sequence.
ACCESSION   DAOOJC010000325 DAOOJC010000000
VERSION     DAOOJC010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28345_length_2765_cov_3.240959"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="PLA01_08465"
     CDS             <1..1083
                     /locus_tag="PLA01_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HPD01366.1"
                     /translation="YGFEETSYLLLFGDLPTQKQLDDFGELLSFYRNLPESFLNDMIL
                     KLPGKNIMNVLARSVLAYYSYDDNPDDTSVKNVLKQCIHLIACMPTIAVYAYQAISHY
                     FRKKSLVIHSPKPELSTAENILRMLRPDKKYSKLEATLLDLALVLHAEHGGGNNSSFV
                     THVVTSTGTDTYSAIAAALGSLKGPKHGGANIKVCEMFEDLKQNIKDWTDDEQIENYL
                     MRILNKEAFDRAGLIYGIGHAVYSISDPRCVLLKEQAEKLAKEKGLEAEFALYDRVEN
                     LAPKVISKVRKIYKGVSANVDFYSGFVYNMLDIPKELYTPIFAISRMTGWAAHRIEEI
                     VNAGKIIRPAYKSVAKRRDYVGIKDR"
     gene            1184..1615
                     /locus_tag="PLA01_08470"
     CDS             1184..1615
                     /locus_tag="PLA01_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01367.1"
                     /translation="MHEYYHSEERQLTPSQGQQPGQQFGPFFGQQFGPLFGPPFGQTP
                     GQQFGPPFGGQGASMGPPSTPPPSFTPQQTQAPGILAVDPGAIRPCTFRFVYIWLNSG
                     ERFWAWLVFVGRTSASGWRWTGFRWIYFGVDLRNIESFVCF"
     gene            2065..2538
                     /locus_tag="PLA01_08475"
     CDS             2065..2538
                     /locus_tag="PLA01_08475"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HPD01368.1"
                     /translation="MKLCPKCGYSNEDFSNVCGSCGSPLNDGAEMPDQQFNQFNTYGS
                     ANMPIQAKTSGFAITSLVLGIVAVPFICCCTYLSIALGIAAAIFGFLAKNEIKSSNGL
                     KKGNGMALAGIILGFVSAAISLILIIYLLAFQDETVSNLNQFFNEFLQEMEEQSN"
BASE COUNT          850 a          456 c          624 g          835 t
ORIGIN      
        1 tatggctttg aggaaacttc gtatttgctc ctttttggcg atttgccgac ccaaaagcag
       61 ttagatgatt ttggagagct tctttcgttc taccgcaatc tgcccgagtc ttttttaaat
      121 gacatgattt taaagctgcc cggaaagaat atcatgaatg tactggcaag aagcgtactt
      181 gcatattaca gttatgatga caatccggat gatacttcag tgaaaaacgt attaaagcaa
      241 tgcattcatc tgatagcatg tatgcctaca attgctgttt atgcatatca ggcaatatcc
      301 cattatttca ggaaaaagag tctggttatt cacagcccga aacccgaatt gagtactgca
      361 gaaaacattc tgcgtatgtt aaggccggac aagaaatatt caaagcttga ggcaacactt
      421 cttgatcttg ctcttgtcct tcatgcagag catggcggtg gaaataattc atcttttgta
      481 acccatgttg taacatccac cggtacagac acatattctg ctattgctgc agcccttgga
      541 tccctcaaag gtccaaaaca tggaggagcc aatataaagg tttgtgagat gtttgaggac
      601 ttaaagcaaa atattaaaga ttggactgac gatgaacaaa ttgaaaacta tctcatgagg
      661 atattgaata aagaggcttt tgaccgggca ggtctgattt atggtattgg gcatgcagtt
      721 tattcgatat ccgatccgag atgtgttctt ttaaaggagc aagccgaaaa acttgcaaaa
      781 gaaaagggtt tggaggcaga atttgctctt tatgacagag tggagaacct ggctcctaag
      841 gttatttcaa aggttcgaaa aatatacaag ggcgtaagtg cgaatgttga tttttactca
      901 ggttttgtat acaacatgct ggacattccg aaggaattat atacaccaat ttttgctatt
      961 tccaggatga ccggatgggc tgcccaccgt atagaggaaa tcgttaatgc cgggaaaatt
     1021 atcaggcctg catataagag tgttgcaaaa agacgtgact atgtgggcat taaggaccgt
     1081 taggaatctc ttaaacaata aagaatttat tgaggctatg gaacagaatt tactgattga
     1141 cataatatgt agtataagac cagacagtta aggagtgaat tatatgcatg aatattacca
     1201 tagcgaggag agacagttga ccccgtcaca gggtcagcaa cccggacagc aattcggtcc
     1261 attttttgga cagcagtttg gaccactctt tggaccgccc tttggtcaga cacccgggca
     1321 gcagtttggg ccgcctttcg gaggtcaggg ggcatctatg ggaccgcctt ccaccccgcc
     1381 gccttcgttt acgccacagc aaacacaagc accgggtata ttggcagttg acccgggggc
     1441 aataaggcct tgcaccttca ggtttgtata tatatggctt aactcaggtg aaaggttctg
     1501 ggcatggctt gtctttgtgg gcagaacatc ggcatcagga tggagatgga caggtttccg
     1561 ctggatttac tttggcgttg acttaagaaa tatagaaagc ttcgtatgtt tctgaccaac
     1621 aggtgaagtg tgttatattc catcattcca aaccggtgaa atctttttta tttgatgatg
     1681 cggattgttt attgagaaat tcttattata taagaagtga gggggcttat gccccttaac
     1741 tttttgtgat tttccacata gcagaatagc taaaaaaggg agaggattac gtatatctca
     1801 gccgttatcc gataaaagca caaccgattg aaatgattgg tgcttgaaaa catgtacatt
     1861 acgagatatt tattaataag ttttagaaaa gtgtaatata ttatattgag gaaaaatgta
     1921 gagagataaa agctgtaagc atagtaaaag aatatgtcga aaatatgcgt agcgatttat
     1981 gaaagtttta tattgataaa aaatcaagaa taatgtaaaa tcattgtgta gttgtattct
     2041 aataattaga cagggggtta acgaatgaaa ctttgtccaa agtgcggtta ttcaaatgaa
     2101 gattttagta atgtatgcgg cagttgtggt tctcctttga atgatggtgc tgaaatgcca
     2161 gaccagcagt ttaatcagtt caatacgtat gggagtgcaa acatgccgat acaagctaaa
     2221 accagcggtt ttgcaattac atccttggtt cttggtattg tggctgtacc ttttatctgc
     2281 tgttgtactt atcttagtat agctttggga atagcagcag ctattttcgg atttttggcg
     2341 aagaatgaaa ttaagtcatc aaatgggtta aaaaaaggaa atggaatggc tttagccgga
     2401 ataatactgg gttttgtaag tgcagcaatt tctttgatat taataatata tttattggca
     2461 tttcaggatg aaactgttag caatctgaac caatttttca atgagttttt gcaggagatg
     2521 gaagagcaaa gcaattaaag cggttatgta aaaattccgg cttttacaac aaagccggtt
     2581 ttttatgtag ttgagacttt aacagcagat atgttataga gaatattaca attaacagtt
     2641 actcttgtgg gtaagtgtta gtttagaggc ttgtttatgg cattccaatt ttatgatccg
     2701 aatcatggaa cagggctatc gcatgaagta atataattcc aaaagtaaat cctgtatatt
     2761 cttaa
//
LOCUS       DAOOJC010000326         2764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_28361_length_2764_cov_3.271318, whole genome shotgun sequence.
ACCESSION   DAOOJC010000326 DAOOJC010000000
VERSION     DAOOJC010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28361_length_2764_cov_3.271318"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1078)
                     /locus_tag="PLA01_08480"
     CDS             complement(182..1078)
                     /locus_tag="PLA01_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014522628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50"
                     /protein_id="HPD01369.1"
                     /translation="MEDRCIFINILKKPFKINIDPVLFILLIIMYYNGYIIRYLLILA
                     SMLIHEGAHITVAVFGKRKLVCVKILAIGLSAEIKEVYDGCINPILIYISGPFINILL
                     ALICFITNTYYFVFSDNMRFFIYANVALAIFNMLPVLPLDGGKIIRDILVTKIGLFKG
                     YKYARVLSLGFTSVVFVLGVLLLLAGIFNLSILLISVYILYYQKFDKTEESLMNIKNL
                     LFKRSRFLKRGIYPGRELVVLKSVHIGDVIKSMDFDRFHIVYIVDESMRLVKVLTEQD
                     VLEGVLKHDPDMRFEDLLKVME"
     gene            complement(1084..1995)
                     /locus_tag="PLA01_08485"
     CDS             complement(1084..1995)
                     /locus_tag="PLA01_08485"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPD01370.1"
                     /translation="MDDIVYRTRYPRQSIPQSRRKTRRSRRTKSSDKYRFSLAQKVRV
                     QIVICMFILLLAGIIKSVNTPVTNFLQHRIKDALSYNIEIKGILNGLNWIIGKNENQA
                     KGQYTTDNSSEGEERTSTGDDMYVTSVDNVFGDPSELDKTEETSDTGMVQSANYNSYD
                     NTEEDEIQAEDEKSFIIPVGGVVGSLYGERIHPISGDVEFHKGIDIEALDGTPIKAAD
                     QGEVMEEGESETYGKYIKLKHDDNTVSLYAHCSSVLVKKGQIVNKGDIIAKVGSTGIS
                     KGAHLHFEIWKNGEAVNPLDFIQSPRN"
     gene            complement(2350..2745)
                     /gene="mgsA"
                     /locus_tag="PLA01_08490"
     CDS             complement(2350..2745)
                     /gene="mgsA"
                     /locus_tag="PLA01_08490"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HPD01371.1"
                     /translation="MNIALIAHDKKKELMASFCIAYKSILKNHTLFATGTTGAIIVEA
                     TGLNINKFLPGLMGEQQISARAAYNEMDLVIFFRDPVTAQSHEPDINSLLRACDMNNI
                     PFATNLGTAEILIQGLDRGDLDWRKIIHK"
BASE COUNT          837 a          622 c          398 g          907 t
ORIGIN      
        1 tgaaaacatt ggattgttga agaaagttaa ggattattaa gtgtgcactg cagcttatga
       61 gtgtgaggaa aaatacacct aaatctatgc ttcttccgta tttttcttca cgttaataat
      121 caatacccga aatggcctaa aaaacaagga gagctttttt caacttcctt tgtttcgggc
      181 attattccat aacctttaac aaatcctcaa atctcatatc cgggtcatgc tttaatacac
      241 cctccagtac atcctgttca gtaagtacct taaccaatct catgctctca tcgacaatat
      301 aaactatatg aaacctgtca aagtccatgc tctttattac gtcccctata tgcacggact
      361 ttaaaaccac cagttccctt ccgggatata tacccctctt taagaatctg cttcgtttga
      421 ataaaaggtt cttgatattc attaacgact cctccgtctt gtcaaacttc tggtaataga
      481 gtatatatac tgaaattaaa agaatgctta aattgaaaat accggcaaga agcaataaca
      541 cccccaacac aaaaactacc gatgtaaatc ctaaagacag taccctcgca tacttatacc
      601 ccttaaacaa tcctattttt gttacaagta tgtctctgat tatttttcct ccgtcaagag
      661 gcaacactgg aagcatgttg aatattgcca atgcaacgtt ggcatagata aaaaaacgca
      721 tattatctga aaaaacaaaa taatatgtgt ttgttataaa acatatcaaa gccagtaaaa
      781 tgttgataaa agggccgcta atgtatatta aaatcggatt tatgcacccg tcatatacct
      841 ctttaatttc cgcactcaat ccgatagcaa gaattttaac acagaccagt tttctttttc
      901 cgaaaacagc tactgtaata tgagcccctt catgaatcag catcgacgct aaaatcaaaa
      961 ggtatcttat tatatagcca ttataataca ttattatcag caatatgaaa agaaccggat
     1021 caatattgat tttaaacggc ttttttaaaa tattgataaa aatacatcta tcctccaaac
     1081 aacttaattt cttggtgatt gtatgaagtc aaggggattt accgcttctc cattcttcca
     1141 tatctcaaaa tgaagatgtg cacctttgga aataccagtg cttcccactt ttgcaatgat
     1201 atctcccttg tttacaatct gccctttctt tactaaaaca gatgaacaat gagcataaag
     1261 ggatacggta ttgtcatcat gctttagctt tatgtatttt ccgtacgttt cactctcccc
     1321 ttcttccata acctcacctt gatccgctgc ctttatggga gtcccatcca atgcctcaat
     1381 atcgattccc ttatggaatt caacatctcc acttattgga tgtatccttt ctccgtataa
     1441 tgaaccaact actcccccca ccggtatgat aaaagacttc tcatcctctg cctgaatctc
     1501 atcctcttca gtgttatcat agctgttata attcgcactt tgaaccattc ctgtatccga
     1561 tgtctcttca gttttgtcaa gctcacttgg gtcaccaaag acattatcga cagatgtcac
     1621 atacatatca tcaccggttg aagtcctctc ttctccttcg ctggagttat cggtcgtata
     1681 ctgtcccttt gcctggtttt catttttccc gattatccag ttcaatccat tcaaaatgcc
     1741 cttaatctca atgttgtacg acagtgcatc ctttatcctg tgttgaagaa aattggtaac
     1801 aggagtatta acgctcttta ttattcccgc caacaacaaa ataaacatgc atattacaat
     1861 ctgtacccta accttttggg ctaaactgaa cctgtattta tcgctgcttt ttgtcctcct
     1921 gcttcttctg gtttttcttc ggctctgtgg tatactctgc ctcgggtacc ttgttctata
     1981 tacaatatcg tccacgaaca gcacatcctc ttattgtttt atagtgttac tctccaagcc
     2041 tttgtctacg gttattgcta caaaacttat aatacataag tctatatatt ttaaattata
     2101 ttaaatatct catgggaata ttacaaggat gaattaaata aatataccca aaaaggttca
     2161 ttagcctttt tttatgcaaa aaaagaacaa ccgattttaa ggtccgttgc tcatttttcc
     2221 tgtgtccctc aaaaaaacaa aagagattat acccataccc tgaccggtca gcatataacc
     2281 ccttcacttg aagctgcaac ttaagtatat actaagcatt gtcatacaat tttgcagtat
     2341 gattttctat catttgtgaa taatttttct ccaatcaagg tcacccctat caagaccctg
     2401 aatcagaatt tctgcagtgc ctaagttcgt ggcaaacggt atgttattca tgtcgcatgc
     2461 ccttaataaa gagtttatgt cgggctcatg cgactgagcc gtaacgggat ctctgaaaaa
     2521 aataaccaga tccatttcat tataagccgc tcttgcactg atttgctgtt cacccattaa
     2581 accgggcaga aacttgttaa tattaagccc ggtggcttca actataattg cccctgtagt
     2641 tcccgtagca aacagagtgt ggttttttag tatgctttta tatgcaatac aaaatgaagc
     2701 cattaattct tttttcttat catgagcaat taaagcaata ttcataaaaa ccttccaaat
     2761 tctc
//
LOCUS       DAOOJC010000327         2754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_28523_length_2754_cov_4.031493, whole genome shotgun sequence.
ACCESSION   DAOOJC010000327 DAOOJC010000000
VERSION     DAOOJC010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28523_length_2754_cov_4.031493"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..1813
                     /locus_tag="PLA01_08495"
     CDS             248..1813
                     /locus_tag="PLA01_08495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPD01372.1"
                     /translation="MSIEKQKIFTKPPQSYWMASTPKTGYPTLEEDIKVDVAIIGGGI
                     TGISTAYLLGKDGVKAAVIEADRILQGTTGHTTAKITSQHELIYSKINNQMGREFAEQ
                     YADANESALRMIEKIATENNIECDFVSQSAYVYTQQDKYIDKIKDEAQIADSLGIKAA
                     YLEEIPLPFPIKAAVRFDNQAQFHPLKFLLPLAKEITESGNQIFEQSRIVDIEDGSNY
                     VLITNQGKKVTAEKIIIASHYPFYNKAGLYFTRLYTERSYVVAVKAKESYPGGMYINS
                     EEPKHSIRSQRSDDGELILVGGGSHKTGQGEDTIKHYDALIDYACETFTVEDIPYRWS
                     TQDCMTMDGLPYVGQFTSNSPNMYIATGYGKWGMTNSMASAMILRDLIVDGKSHWQDV
                     YNPSRKTIAASAKNFVVENLNVAEKLIEGKISPSQDDIGVRAGEGKIIKANGQRLGAY
                     RDEQGELHVVDTTCTHMGCELYWNSAEKSWDCPCHGSRFSYEGDVIEGPAVNSLNAHN
                     DVNTIEKLIKDNF"
     assembly_gap    1823..1832
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1973..>2754
                     /locus_tag="PLA01_08500"
     CDS             1973..>2754
                     /locus_tag="PLA01_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPD01373.1"
                     /translation="MKNRELNKVRPIKDLKDMLNSSVEIYGDKAAFLSKPKGGTAYEP
                     ISYKQFKSDVDAMGTALIDLGLKNGKIALIGENRYEWSISYLSVVNGTGIIVPLDKEL
                     PANEIELLLQRSQADAVIYSESVRDKIEPLYKKSTSLRYFISMDREEDDGSILSFHLL
                     LQKGHKLLEEGRREFIDAKIDACAMSILLFTSGTTDTAKAVMLSHKNIAENLMGMCSM
                     VYIDEKDIFLSILPIHHTYECTCGFLCQLYRGCTIAYCDGLR"
BASE COUNT          894 a          502 c          566 g          782 t
ORIGIN      
        1 ctgagacacc attgtctcaa ttttgttgac ccataagtat tataaattta aatttccctc
       61 ttgtcaattt ttttatccaa aggcaagtgc attaatttaa tgcatactga tttattgtaa
      121 tttgtcacag ctttgttacc aaaacagaga atcatcccct gttttatgga tatttcagca
      181 tctacctgca aagaataaat aatactatat agattgcttg atttctgaat tatcggaggt
      241 agtttttatg agtatagaaa aacaaaagat ttttaccaaa ccgccccagt catattggat
      301 ggcatccact cctaaaaccg gttatccaac cttagaggag gatataaagg ttgacgtagc
      361 aattatcgga ggcggtatta ccggtatctc cactgcatac ttacttggca aagatggagt
      421 aaaagcagct gttattgagg ccgaccgcat tttgcagggc acaaccggac ataccacagc
      481 aaaaataacc tctcagcatg aattgatata cagtaaaata aataatcaga tgggcaggga
      541 attcgcagaa caatatgccg acgcaaacga gtccgccctt cggatgattg agaaaatagc
      601 aactgaaaac aatatcgaat gcgattttgt ttcccaatcc gcatatgtgt atacgcagca
      661 agacaagtat atcgataaga taaaggatga ggcacagatt gccgattccc ttggcataaa
      721 agctgcttat cttgaagaaa tacctctgcc tttcccaatt aaagctgctg tacgttttga
      781 caaccaggct caattccatc ctctaaaatt tctattgcct cttgcaaaag aaattaccga
      841 aagcggtaat caaatatttg agcaaagcag gattgtagac attgaagacg gtagtaacta
      901 tgttttaata acaaatcaag gtaagaaggt aactgcagaa aaaatcataa ttgcttccca
      961 ttatcctttt tacaataagg ccgggttata ctttacaaga ctttatacgg aacggtcata
     1021 tgttgttgct gttaaggcaa aagaaagtta tcccggtgga atgtatataa attcggaaga
     1081 accaaagcac tccatacgca gccaaaggtc agatgacggc gaacttatac tggtcggcgg
     1141 cggaagccac aaaaccggtc aaggcgagga cacaatcaag cattatgatg cattgataga
     1201 ttatgcctgc gaaaccttta cagtggagga tattccttat cggtggtcta cccaggattg
     1261 catgactatg gacggactgc cttatgtggg gcaattcaca tccaactctc ccaatatgta
     1321 cattgcaacc ggttatggca aatggggaat gactaacagt atggcttctg cgatgatatt
     1381 aagggacttg atagttgacg gaaaaagcca ctggcaggat gtctataacc cttcacgcaa
     1441 aactatcgca gcttccgcta agaattttgt tgttgaaaat ctcaatgttg cggaaaagct
     1501 aattgaggga aaaatttcac cctcacagga tgatatcggt gttagagctg gagagggaaa
     1561 aattattaag gcaaacgggc aaagacttgg agcatataga gacgaacaag gagaattgca
     1621 tgtggtagac acaacatgca cgcatatggg ctgtgaatta tattggaatt ctgccgagaa
     1681 atcctgggat tgtccttgcc atggctcaag attcagttat gaaggcgatg taattgaagg
     1741 cccggcagtc aactctttaa atgcacacaa tgatgttaat acgattgaaa aacttattaa
     1801 agacaatttt taaagtgaat agnnnnnnnn nnggacgtat ctctgtttta gaacagaaaa
     1861 ctgtcccctg ttttatctat tattcgtttt gttttgcaaa cactgccaat agtattgtat
     1921 aattattatt gttgctttgc aggacacaat ctttttttgg gggaaattta aaatgaaaaa
     1981 cagagagtta aataaagtaa gacctattaa ggatttaaaa gatatgctta attccagtgt
     2041 agagatatat ggtgataaag cggcgttttt atctaaaccg aaaggtggaa ccgcttatga
     2101 acccatatca tataaacagt ttaaatccga tgtggacgct atgggaacag ccctaattga
     2161 tcttggtcta aagaatggga aaattgcttt aataggtgaa aaccgatatg aatggtccat
     2221 atcctatctt tcagttgtaa atggaacagg aattattgta cctcttgata aagaacttcc
     2281 tgcaaacgaa attgagttac tcctgcaacg ttcacaggca gatgctgtca tttattcgga
     2341 gagtgtcagg gacaaaattg agcctcttta taaaaaatct acttccttac ggtattttat
     2401 cagtatggac agagaagaag acgatggcag catcttatct ttccatttgc ttttacaaaa
     2461 ggggcataaa ttattggaag aaggaaggcg agagtttatt gatgccaaaa ttgatgcctg
     2521 tgcaatgagt atccttctct ttacttccgg aaccaccgac actgccaagg ctgttatgct
     2581 gtctcacaaa aacatagcag aaaacctgat gggaatgtgt tccatggtat atattgatga
     2641 aaaggacata ttcttatcga tccttcccat tcatcatacc tatgaatgta cctgcggttt
     2701 tttatgccaa ttatacaggg gatgtaccat tgcatattgt gatggattgc gaca
//
LOCUS       DAOOJC010000328         2754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_28528_length_2754_cov_3.190070, whole genome shotgun sequence.
ACCESSION   DAOOJC010000328 DAOOJC010000000
VERSION     DAOOJC010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28528_length_2754_cov_3.190070"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..304)
                     /locus_tag="PLA01_08505"
     CDS             complement(173..304)
                     /locus_tag="PLA01_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01374.1"
                     /translation="MKDINTIDSSHMESFVLVLALIIKMPFDMCMREHIEGIFLGKI"
     gene            complement(356..1165)
                     /locus_tag="PLA01_08510"
     CDS             complement(356..1165)
                     /locus_tag="PLA01_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPD01375.1"
                     /translation="MIEKVFNTQNGVIHYRTSIVSGEKTWLVFLPGLTADHRLFEKQI
                     EEFKDDYNLFVWDAPGHGASRPFELDFSLADKAEWLYSIMIHEGIFKPIIIGQSMGGY
                     VAQALMERYPDCLAGFVSVDSAPLKRKYMKKWEIYVLKNTKFMFGVYPWFFLKKYAAK
                     GCAQTEYGRQVMYAMMDTYTKREYCDLSSHGFKILAEAIEADLAYEISCPALLICGEK
                     DKAGFTKRYNKAWANQSGLPIQWIPGAGHNSNTDKPELVNSVIREFLGTIL"
     gene            complement(1266..1919)
                     /locus_tag="PLA01_08515"
     CDS             complement(1266..1919)
                     /locus_tag="PLA01_08515"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPD01376.1"
                     /translation="MVNIKLWMECFTQILKETFGERIYFVGLQGSYGRGEATESSDID
                     VVVILDKLTTDDIKAYSDMIDTLPNRELICGFLSGKDELINWEPSDLFQFYYDTTPII
                     GTLDELWTKIDNAAVDRAIKIGACNIYHGCVHNMLYEKSDDIVRGLYKSASFVIQAIV
                     FNNTGKYVKHQKDLLEAVNNEDKAILNDFIALKNGAMVEFDEMSERLYNWAGKLIKI"
     gene            complement(2019..>2754)
                     /locus_tag="PLA01_08520"
     CDS             complement(2019..>2754)
                     /locus_tag="PLA01_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD01377.1"
                     /translation="LAGDVRLFEVDFEYEPGKTVLHDVSLYAKPGQKVAFVGATGAGK
                     TTITNLLNRFYDIADGKIRYDGININKIKKPDLRRAIGMVLQDTNLFTGTVMDNIRYG
                     KLDAADEECIGAAKLVGADDFIRRLPNGYHTLITENGANLSQGQRQLISIARAAVADP
                     PVMILDEATSSIDTRTEAIVQRGMDALMKGRTVFVIAHRLSTVKNSDVIIVLDHGRII
                     ERGDHEELIAKKGQYYQLYTGAFELE"
BASE COUNT          777 a          637 c          456 g          884 t
ORIGIN      
        1 ttcatcgttt ttttcttgcc gagctctttt taagatgtct ttgttattca tttatatccc
       61 ctcccaggat ttatgcttat attatatact atgaatagta ttgcgtcaag tttacttgtc
      121 aaaatgcaaa gttaagttgt cattatttct gttattttaa ataatagggt tttcaaattt
      181 tgcctaaaaa tataccctcg atatgttccc gcatacacat atcaaagggc atttttatga
      241 ttaaggccag aaccaacaca aaagactcca tatggctgga gtcgatggta ttaatgtctt
      301 tcatcttaga aaccgtctgt taaccggaat ctgtcaactc acatatttgg aacagctaca
      361 gtatagtccc gagaaattca cggattacac tgttgaccag ttcaggtttg tcagtattgg
      421 aattgtgtcc ggccccgggt atccattgaa ttggcagacc tgactgattt gcccatgctt
      481 tgttgtagcg ttttgtaaaa ccggctttgt ctttctcacc acatatcaac aacgctgggc
      541 aggagatttc atatgccaga tccgcctcaa ttgcttctgc cagaatcttg aatccatgag
      601 acgacaggtc acaatactct ctttttgtat aagtatccat catagcatac ataacctgac
      661 gtccatattc ggtttgtgca catccttttg cagcgtattt ttttaaaaag aaccatggat
      721 aaacaccgaa catgaatttt gtatttttga gtacatatat ctcccacttt ttcatatact
      781 tccttttaag cggagcagaa tcgacagaaa cgaagcccgc aagacaatcc ggatatctct
      841 ccattaatgc ctgagcaaca taacctccca tagactgtcc tataattatt ggtttgaata
      901 ttccctcatg aatcatgata ctgtagagcc attctgcttt atctgcaagc gaaaaatcga
      961 gttcaaacgg tcttgaagca ccatgccctg gtgcatccca tacaaacaaa ttgtaatcat
     1021 ctttgaattc ttcaatctgt ttttcaaaca atcgatgatc tgctgtcagc cctggaagaa
     1081 atacaagcca cgtcttttcg ccggaaacaa tgctggtccg gtagtgaatt accccgtttt
     1141 gggtattgaa cactttctct atcattctcc atcctcctcg tcaagataaa acaatgccat
     1201 tcctgcattg cagcaatagc ccttgatgaa gtatccttcc tgaattgctg caaagaaaga
     1261 gggcttcata tctttattag cttccctgcc cagttatata aacgctccga catttcatca
     1321 aattctacca tagcaccgtt ttttaaagca ataaagtcat ttaaaatcgc tttatcctca
     1381 ttgtttacag cttcaagcaa atctttttga tgctttacat attttcctgt attattaaat
     1441 acaatagcct gtatgacaaa agatgccgac ttatatagcc cacgcactat atcatcgctt
     1501 ttttcatata acatattatg tacacatccg tggtaaatgt tgcatgcacc tatttttata
     1561 gcacgatcta ctgctgcatt atcaattttt gtccacagct catcaagggt acctataatc
     1621 ggtgtggtat cataataaaa ctgaaataag tctgaaggtt cccaatttat aagctcatct
     1681 tttcctgaaa gaaatccgca aattaattcc cggttaggaa gggtatctat catatcgcta
     1741 tatgctttta tatcatctgt cgttaattta tccaatatca ctactacatc aatatcactg
     1801 gattcagttg cttctcctct tccgtaactt ccttgcagcc ctacaaaata aatacgttca
     1861 ccaaatgtct ctttaagtat ctgtgtaaag cactccatcc acaattttat gttaaccatc
     1921 ataaccctcc attttttatt tctttccaca tggtttcttt tctggcaacg attatacgat
     1981 tataatagtt ttcaacaaaa tagaaggggt attatatttt actccagttc aaatgcaccg
     2041 gtatacagct gataatactg acccttttta gctatcaatt cctcatgatc gccccgttca
     2101 atgatacgtc catgatccaa aactatgata acatcagagt ttttaacagt ggacagacgg
     2161 tgagcaatta caaacactgt cctgcctttc atgagggcat ccattccccg ctgaacaatg
     2221 gcttcagtcc ttgtatcaat ggaggacgtc gcttcatcca aaatcataac cggaggatcc
     2281 gcaaccgctg ctctggcaat ggaaattaac tgcctctgtc cctgagatag attcgcaccg
     2341 ttttcagtaa tgagcgtatg atagccattg ggcaaacggc gaatgaaatc atcggcacct
     2401 acaagctttg cagccccaat acactcctca tcggcagcat ccagttttcc gtaacgtata
     2461 ttgtccatta ctgtgccggt aaaaaggttg gtatcctgca aaaccatgcc gatggcacga
     2521 cgaaggtccg gttttttgat tttattgata ttaatgccgt cataacggat tttcccgtcg
     2581 gcaatatcat aaaatcggtt cagcaaattt gttatagttg tctttccggc accggtggca
     2641 ccaacaaagg ccactttttg tcccggcttt gcgtaaaggc tgacatcgtg caaaacagtt
     2701 ttccccggtt catattcgaa atcaacctcg aacaatctca cgtcaccggc cagt
//
LOCUS       DAOOJC010000329         2738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_28762_length_2738_cov_3.352218, whole genome shotgun sequence.
ACCESSION   DAOOJC010000329 DAOOJC010000000
VERSION     DAOOJC010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28762_length_2738_cov_3.352218"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1292
                     /locus_tag="PLA01_08525"
     CDS             120..1292
                     /locus_tag="PLA01_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HPD01378.1"
                     /translation="MDYRKESLRLHGEWKGKIEVISKVPTKSKEDLSLAYTPGVAEPC
                     LAIQKDVNLSYTLTRRWNLVAVITDGTAVLGLGDIGPEAGMPVMEGKCVLFKQFGDVD
                     AFPLCIKSKDVDEIVNTVKLISGSFGGINLEDISAPRCFEIERRLKEVCDIPVFHDDQ
                     HGTAVVSLAAMLNALKLTGKNINDIEVVVNGSGAAGIAVTRLLMSMGLKKVILCDRKG
                     AIYEGRDDLNEEKALMAKISNLEKKKGSLAEVIKGADVFLGLSAPGMVTQDMVKTMAK
                     DPIIFAMANPTPEIMPDEAIAAGAKVVGTGRSDFPNQINNVLAFPGIFRGALDVRASD
                     INDEMKIAAAKAIASLVSDEDLCPECILPEPFDPRVGSTVAKAVAEAARITGVARI"
     gene            complement(1331..1498)
                     /locus_tag="PLA01_08530"
     CDS             complement(1331..1498)
                     /locus_tag="PLA01_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01379.1"
                     /translation="MNNMEHNAGFFNDYHAQIDGVGSKFCSGMEPNCSKCSLESVCKK
                     SDWTIEKERGT"
     gene            complement(1545..>2738)
                     /locus_tag="PLA01_08535"
     CDS             complement(1545..>2738)
                     /locus_tag="PLA01_08535"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01380.1"
                     /translation="LLVPVIPLAISSLLVIVLMRFVNIKKTKDVIVVVASFLSIAIGM
                     GVNFYVQRMINGGMDEYLDMSQIQNWVIEAVGSRFPPSLWVTYGLSKPGMEGWGYFLL
                     FVLVSVALFTLLLWVGNRVFYKGILSGNERSGKKRRVSFENSMNKYIKVTSPLTALFW
                     KEWKLLLRTPVFMINGLAGVVVMPVMTLISILGQNQESIGKFIEVAREPRNDIYVSLV
                     GFGLMVLASSLNIVSSTAVSREGHSIWISKMIPVSPRMQIISKLIHSMSISMMGSLVI
                     AGFMVALIRVSVLKVLFLIILSSLGSMTILILDLIIDIFRPKLDWTNPHEAVKTNLNG
                     LFGIIVSMGFMLILTVFASAMVLLQLPQTVVFLGIFIFEAVMLLLSIIVLFKPAEKRY
                     RNIEA"
BASE COUNT          890 a          558 c          555 g          735 t
ORIGIN      
        1 aaccgcttta atgtattctg tagatcaggt aaaaagtatt ctaaatgaag taaaggacat
       61 gtaaaataga atacattcgg ctaagattat gtatataata actttaggag gttaatataa
      121 tggactacag aaaagaatca ttaaggctgc acggtgaatg gaagggcaaa attgaagtaa
      181 tcagtaaagt tccgacaaaa agtaaagaag atctctccct ggcttataca ccaggagtag
      241 cagaaccgtg tcttgctata cagaaggatg taaacctttc atatacactt acaaggcgtt
      301 ggaatctggt agcagtaata accgatggca cagctgttct tgggcttggt gatattggtc
      361 ctgaagcggg catgcctgtt atggaaggta aatgcgtact ctttaaacag tttggcgatg
      421 tagatgcatt cccattatgc ataaagtcta aggatgtgga tgaaatcgtc aataccgtaa
      481 aactgatatc gggaagcttt ggaggaatca atctcgaaga catatctgct ccaaggtgtt
      541 ttgagattga aagaagactt aaagaagtat gcgatattcc ggttttccac gatgaccagc
      601 acggtacagc cgtagtatcc cttgctgcaa tgctgaatgc actcaaactc acaggaaaaa
      661 acattaacga cattgaggtt gtagttaacg gttctggtgc tgcaggtata gctgttacaa
      721 ggcttctcat gagcatgggc ttaaagaagg ttatactctg tgacaggaaa ggtgcaatat
      781 atgaaggaag agatgacctc aatgaagaaa aggccctgat ggctaaaata tcaaaccttg
      841 agaaaaagaa aggcagcctt gccgaagtaa taaaaggtgc cgatgtgttc ctcggtcttt
      901 ctgccccggg aatggttact caggatatgg tcaagaccat ggcaaaggat ccgataatat
      961 tcgcaatggc aaatcctaca cctgaaataa tgcctgatga agctatagca gcaggtgcaa
     1021 aggtggtagg taccggaaga tccgatttcc cgaaccagat aaataatgtt ctggcttttc
     1081 ctggtatatt cagaggtgca ttggatgtta gggcaagtga tatcaacgac gaaatgaaga
     1141 tagctgctgc taaagcaatt gcttctttgg tcagcgatga agatctttgt cctgagtgta
     1201 ttctgccgga accctttgat ccgagggttg gttccacagt agcaaaggca gtagctgaag
     1261 cggcaagaat aactggagtt gcaagaatat aaggttgtaa agataaaaat ggttaggaga
     1321 gaggcaattt ttatgttcct ctctcctttt caattgtcca atccgatttt ttacaaacac
     1381 tttcaagaga gcatttactg cagttgggtt ccattcccga acagaatttg cttcccacac
     1441 cgtcaatctg tgcatgataa tcattaaaaa atcccgcatt atgctccata ttgttcataa
     1501 aaaagctctg catttccttg aacgcttata tcaagcctcg tcttctacgc ttcgatattt
     1561 ctatatcttt tctcagccgg tttaaataaa acaatgatac ttagaagcag cattacagcc
     1621 tcaaaaatga atattcccaa gaagaccact gtttgcggca gctgcaatag aaccattgcc
     1681 gatgcaaaga cagttagaat cagcataaag cccattgaaa ctatgattcc aaaaagcccg
     1741 ttaagattgg tttttacagc ctcgtgagga tttgtccaat caagtttggg tctgaatatg
     1801 tcaataatta aatcaaggat taaaattgtc attgaaccca aagaagaaag aataattagg
     1861 aataaaacct tcagtacgga aacccttatg agggcaacca tgaaccccgc aataacaagg
     1921 cttcccatca ttgatataga catactgtgt ataagcttcg aaattatctg cattcgcggc
     1981 gaaacaggaa tcattttgga tatccagatg ctgtgtcctt ctctcgaaac agctgttgac
     2041 gatacaatat tgagacttga agcaagaacc attaatccaa agcccaccag cgacacgtat
     2101 atatcgtttc gaggctcccg agcaacttct atgaattttc ctatggattc ttgattctga
     2161 ccaagaattg aaattaaagt catcacaggc ataactacca cacctgccaa tccatttatc
     2221 ataaacacgg gggtccttaa aaggagcttc cactctttcc agaaaagagc ggtcaatggg
     2281 ctcgttactt taatatactt attcattgaa ttctcaaaag aaacacgtct ttttttgccg
     2341 gatctttcat ttcccgataa aatcccctta taaaacaccc tgttgcctac ccataacagc
     2401 aaagtaaaca atgcaacgga aaccagcaca aaaagtagaa agtaacccca tccttccatg
     2461 cctggtttcg aaagaccgta ggtaacccaa agactgggcg gaaaccttga ccctacagct
     2521 tcaataaccc agttctggat ttgactcatg tccaaatact catccatacc gccatttatc
     2581 attctctgaa catagaagtt tactcccatt cctatggcaa tggacagaaa actggctact
     2641 accactatta catccttggt tttcttaata ttaacaaacc tcatgagaac tattacaagc
     2701 aaagaggata ttgccagagg tattaccggc acaagcaa
//
LOCUS       DAOOJC010000330         2723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_28984_length_2723_cov_3.795727, whole genome shotgun sequence.
ACCESSION   DAOOJC010000330 DAOOJC010000000
VERSION     DAOOJC010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28984_length_2723_cov_3.795727"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PLA01_08540"
     CDS             complement(<1..783)
                     /locus_tag="PLA01_08540"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HPD01381.1"
                     /translation="MTSNKKLQAWVDEIAKMCEPDEIYWCDGSEEENERLLKKLVESG
                     SATPLNPEKRPGCYLFRSHPSDVARVEDRTFIASKKEEDAGPTNNWIDPEELKKTMKG
                     LYTGCMKGRTMYVIPFSMGPIGSPISKIGVEITDSAYVVVNMRIMTRIGSKVLEVLGD
                     DGEFVPCLHSVGAPLEEGQKDVSWPCAPMEKKYISHFPEERAIWSYGSGYGGNALLGK
                     KCFALRIASVLARDEGWMAEHMLILRITDPNGNKTYVTGAFPS"
     gene            complement(1030..1797)
                     /locus_tag="PLA01_08545"
     CDS             complement(1030..1797)
                     /locus_tag="PLA01_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPD01382.1"
                     /translation="MEYTVKKLAALAGISARTLRYYDEFGILKPARINSSGYRIYGQK
                     EVDTLQQILFYRELGVELERIKEIITSPEFDMTSALIEHREKLLAKRRQLDLLIANVE
                     KTIAFAEGRTQMSDKEKFEGFKQKMIDDNEKKYGKEIREKYGDEVVEQSNQRFKNMTE
                     EDFDRLKGLESEIIETLKEAFATGDPAGELAQKAADLHKQWISCSWGSYSKEAHAGLA
                     QMYVDDERFTAYYDKEQPGMAKFLRDAILIYTGMKEK"
     gene            complement(1938..>2723)
                     /locus_tag="PLA01_08550"
     CDS             complement(1938..>2723)
                     /locus_tag="PLA01_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPD01383.1"
                     /translation="QDEEEVTEEEIRMMVDVGEERGAIQENEKEMINNIFDFDNKTAM
                     DIMTHRTDIVALPINAGLKEVISVLNEEKYTRIPVFEGSIDNIVGIIHAKDIIKFIDC
                     NQDKEDFDLRKLIRHPYNVPWSKRTDELFSELQKNKNHMAIIIDEYGGTAGIVTMEDL
                     VEEIVGNIFDEYDEEEKDFEKLDENTYMASGSVGLDVVKEQFDAMLPVEEYDTLSGFV
                     ISQLGRIPENGERPEIEFNGLLFKVVEISEKRIEKIKICKVTN"
BASE COUNT          738 a          660 c          408 g          917 t
ORIGIN      
        1 acttgggaaa gcacctgtaa catatgtctt gttaccatta gggtcggtta tgcgaagaat
       61 gagcatatgc tcagccatcc atccctcgtc ccttgccaaa acagaggcaa tacgaagtgc
      121 aaaacatttt ttgcctaaaa gtgcatttcc gccatatccg gaaccatagg accatatagc
      181 cctttcttca gggaaatgac tgatgtactt tttctccatt ggtgcacatg gccaagacac
      241 atctttttga ccttcctcta atggagcacc taccgaatgc agacacggca caaattcccc
      301 atcatcacca agcacttcaa gaaccttgct gccaatacgg gtcattatac gcatgttcac
      361 aacaacataa gcactgtcag ttatttcaac acctattttg gaaataggtg aaccgattgg
      421 gcccatggaa aacggtataa catacatggt tctacccttc atgcagccgg tgtataagcc
      481 cttcattgtc tttttcaact cttccggatc aatccagttg ttggtaggtc cggcatcttc
      541 ttctttctta gaagcaataa atgttctgtc ctcaacacgg gctacatctg acggatgact
      601 gcggaacaag taacatcccg gacgtttttc tgggttcaga ggtgtagctg aacctgattc
      661 aactaacttc tttaacaggc gttcattttc ttcctctgag ccatcgcacc aataaatttc
      721 atcgggctca cacatttttg caatttcatc tacccaagct tgcaattttt tgtttgatgt
      781 catcaaaatt ctccctccaa tttttagata aactatctat taataaacta atctattgct
      841 gattggcaat acatagtaga caacaatgaa atcagcattt atgcaatata atgaattagt
      901 taattttttc actaacatcc taacacaaat tcaataaaat atcaacacgt gttttcgaat
      961 ttttgctttt tgggagggga gaattgaatg aaggtgaaga aaaataatat ttatccatgc
     1021 aaatggaaat tacttttcct tcattccagt gtatatgaga atagcatccc taagaaattt
     1081 agccattccc ggctgttctt tatcgtagta tgccgtaaac cgctcatcat caacatacat
     1141 ttgggcaagc cctgcatgag cttctttgct gtagcttccc caggagcagc ttatccattg
     1201 tttatgcaaa tccgccgctt tttgggcaag ctctcctgcc gggtccccag tagcaaaagc
     1261 ctccttaagg gtttctatta tctccgactc taaccccttc aacctgtcaa aatcctcttc
     1321 ggtcatgttc ttaaagcgtt ggttggactg ttccaccacc tcatcaccgt atttttcccg
     1381 gatttcttta ccatactttt tctcgttatc atcaatcatt ttttgcttaa agccttcaaa
     1441 cttttcctta tcactcattt gcgttctccc ttctgcaaat gctatggttt tttcaacatt
     1501 tgcgattaat aaatccagtt gtctcctttt cgcgaggagc ttctcacggt gttcaatcag
     1561 tgcagaggtc atgtcaaact cgggtgatgt tataatttct ttgatgcgtt ccaactccac
     1621 acccaactcc ctgtaaaaca atatttgctg cagcgtgtca acctctttct gtccgtatat
     1681 ccgatatccg gatgaattta ttcttgccgg cttcagaatt ccaaattcat cataatacct
     1741 gagggtacgg gcactgattc cagccagagc tgccagtttc ttaactgtat actccatgtt
     1801 tttacctcct cacaatacca ttttacacct ttacgttacg taaaggtcaa ggggtagatt
     1861 cacaaatatt aaatcaaaga taagtatttg tttgtgaaaa attcccttat atattgatac
     1921 aaatttatgg aaaacatcta atttgttact ttgcaaatct taattttttc tatcctcttt
     1981 tcacttattt ccacaacctt aaataaaagt ccgttaaatt ctatttccgg tctttctcca
     2041 ttctccggta ttctgcccaa ttgacttata acaaaaccac ttaacgtatc atattcctca
     2101 acaggtaaca ttgcatcaaa ttgttcctta acaacatcaa gacctacaga accactggcc
     2161 atgtacgtgt tttcgtccag tttctcaaaa tccttttctt cttcgtcata ttcgtcaaat
     2221 atatttccca ctatctcctc cacaagatct tccatggtta ctattcctgc tgttcctcca
     2281 tactcgtcaa taattatagc catatgattc ttgttctttt gtaattcgct gaaaagctca
     2341 tccgttctct ttgaccacgg aacattatac ggatgcctta ttagtttcct taaatcaaaa
     2401 tcttctttgt cttgattgca atctataaac ttaataatat cctttgcatg aattattcct
     2461 acaatattgt caatactacc ttcaaaaaca ggaattcttg tatatttttc ttcgttgaga
     2521 acagaaatca cttctttaag tcccgcatta atcggaagag ccacaatatc ggtcctatgg
     2581 gtcattatgt ccatggcagt cttgttgtca aaatcaaaaa tgttgttgat catttccttt
     2641 tcattttcct gtatggcacc cctctcctca cctacatcca ccatcatacg aatctcttcc
     2701 tccgtaactt cttcctcatc ctg
//
LOCUS       DAOOJC010000331         2722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_28994_length_2722_cov_4.875891, whole genome shotgun sequence.
ACCESSION   DAOOJC010000331 DAOOJC010000000
VERSION     DAOOJC010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28994_length_2722_cov_4.875891"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(789..864)
                     /locus_tag="PLA01_08555"
     tRNA            complement(789..864)
                     /locus_tag="PLA01_08555"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(829..831),aa:Lys,seq:ctt)
     gene            1049..1135
                     /locus_tag="PLA01_08560"
     tRNA            1049..1135
                     /locus_tag="PLA01_08560"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1083..1085,aa:Leu,seq:caa)
     gene            1240..1632
                     /locus_tag="PLA01_08565"
     CDS             1240..1632
                     /locus_tag="PLA01_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPD01384.1"
                     /translation="MQEVYEFLKNCGTYYLATVEGDQPRVRPFGTVDIFEGKLYIQTG
                     KVKEVSKQLQSNPKAEICAYTDGKWLRVEGKLVRDDRVEAKKHMLDNYPELKTMYSAE
                     DDNTEVLYFVNATATFYSFTDESKVVKF"
     gene            complement(1833..>2722)
                     /locus_tag="PLA01_08570"
     CDS             complement(1833..>2722)
                     /locus_tag="PLA01_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01385.1"
                     /translation="LSRVLENPKEAEELLIKSIEGDDPCPYGYHGLAYQRLSEGNFEQ
                     ALQYAQKAVEALSNQNTFNMVLEEAMWAQGEYDLLLKKNEEQQILYPYDGSVVEESVR
                     LHMAKGDLNSAQNAISDYLLRNEPMGSEYNQVWNNYLRGILAYCSGDVAAYSNSIEEL
                     EAPQYNFEKAFIKGDFENASKIAADNGLDGSYFLLLYLAESNPDSASRYLSKAADMYN
                     KGGKTERLLAAYLSGTKSITLDEVKSIDLLPDSKCIALAAIGKLNPDYQEELYTFAKK
                     LNYKKVFPYHFINELGKDS"
BASE COUNT          830 a          550 c          494 g          848 t
ORIGIN      
        1 aatccgtttt cgataagccg aggcctggca ctgatcatgc gtgaaaaggc ttcaatcatc
       61 aaatcctcag cctcatgaat gtcatctatg tacctattaa tatagagtgt taaagcatct
      121 ccgtggcgtt ccatgagaac gcgtaaggca ctcccgtcac cgtcgaggta tgcagaaaaa
      181 agttcttcgt ttgctgcttc atctgttgtt ataacaatcc ctcctgttca cctgcatttg
      241 cgtaagtcag aaaactttta tgtctctaat tttccacccg ttatatcaac gcaacacact
      301 ctaaatgccg tgagacagtt gcattgatgt ttatagtttt tatattcgat atatttctac
      361 tcaatattga ttatacgtat tacctctctc taaatgcaat attaaaacac aacaatatta
      421 taataagagg atatactttt tatgaaaacc ataataacct tatttactgt ggatttggaa
      481 atgcactctg ccaaatgtta tgtagagttc caaattcctt ttgatttaag ctgcatagat
      541 cggattatgc agtatcaata atgacttctt taaatattcc caagatatca atccaataaa
      601 atctgcttcg caatttgcag aaatctttct ttatatgtat atagaatgga caaaataaga
      661 attattcgtg ttttataaaa caaaagttcc ggggcatcga tcacattcgc ttttcggtca
      721 tttcacttcg tgaaatccca ctaaaaaaga tgttgaaaca taattgcttc aacatctttt
      781 tttcaatttg gtgcgccatc ggggactcga acccagggcc cactgattaa gagtcagttg
      841 ctctaccaac tgagctaatg gcgcatatac tttcgttcgc cgaacaatta tatattataa
      901 gagcaaaatg attcattgtc aacacaaaat attaaaattt ctttttagtt atttttttct
      961 catccgatgg taaaaaaatc ttttcgaaat taattgacat agggaacttt attgttgtat
     1021 aatacttctt gcggtgaata aagtatatgc cgaagtggtg aaattggcag acgcactgga
     1081 ctcaaaatcc agcggtagcg ataccatgcc ggttcgagtc cggccttcgg caccaaaggg
     1141 gtttgagaga gtttattatt ttctcaaacc ctttaacttt atttgatttt ctaaaagact
     1201 tctaacgcct tttatcagac aaacgggagg aatgataaga tgcaagaagt atatgagttt
     1261 ttaaaaaact gcggaacata ttatttggct acagtggaag gggatcagcc cagagttagg
     1321 ccgtttggta cagtggatat ttttgaaggc aagctttata tacagacagg aaaggtaaaa
     1381 gaagtatcca agcagcttca gtcaaatcca aaagcggaaa tctgtgctta tactgacgga
     1441 aagtggctta gagtagaagg caagcttgtc agagatgaca gagtagaggc taaaaagcat
     1501 atgcttgaca attatccgga gcttaaaact atgtattcag cggaggacga caatacagag
     1561 gtactctatt ttgtgaatgc cacagctact ttttattcct ttaccgatga atcaaaggta
     1621 gttaagtttt agtagtgagc gttttgacaa tgtaaatatg gggtaatctt tcccgcaccg
     1681 gaaggaatac cgcaatatta gtgggatttc acgaagtgaa atgaccaaaa agcgatgggg
     1741 gctcgatgcc ccgagctcat ttttagaatt caccattgca aaataaaaac ggaggagcat
     1801 taaatacctc ctccatttca cattaaaaac ttctagctgt ctttacccag ttcattaata
     1861 aaatgatacg ggaatacttt tttgtagttg agttttttag caaaggtata aagctcctct
     1921 tgataatccg ggtttaattt acctattgct gccaatgcaa tacactttga atccggcagt
     1981 aaatctatcg atttgacttc atccaatgtg atgcttttgg ttcccgacag gtaagcggct
     2041 aacagtcgct cagtcttacc tcctttatta tacatatctg ctgccttact gagataccgg
     2101 gaggcagaat ccggattgct ttctgccaaa taaagcaaga gaaagtatga cccatcaagg
     2161 ccattatcag cagcaatttt tgaagcattt tcaaagtccc ccttgataaa tgccttttca
     2221 aagttatatt gaggtgcttc cagttcttca attgaattgc tatatgcagc cacatcaccg
     2281 gaacaataag caaggattcc tctcaaataa ttattccata cttgattata ttccgaaccc
     2341 atcggctcat tgcggagcaa ataatcagag atagcatttt gggcactgtt caaatcccct
     2401 ttagccatat gtaaacgcac cgattcctct accacgctac cgtcataggg atagagaatt
     2461 tgctgctctt cattcttttt caacaataaa tcatattccc cttgtgccca catagcttct
     2521 tccagaacca tattaaaggt attctgattg gataaagctt ccacagcctt ttgtgcatat
     2581 tgcaacgcct gttcaaagtt tccctcggac agcctttgat aagccaaacc atggtagcca
     2641 tacgggcatg gatcatctcc ttcaattgac ttaatcagca gctcctctgc ttcttttgga
     2701 ttttctaata ctctacttaa aa
//
LOCUS       DAOOJC010000332         2722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_28998_length_2722_cov_2.986502, whole genome shotgun sequence.
ACCESSION   DAOOJC010000332 DAOOJC010000000
VERSION     DAOOJC010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28998_length_2722_cov_2.986502"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..613
                     /locus_tag="PLA01_08575"
     CDS             167..613
                     /locus_tag="PLA01_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015550797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPD01386.1"
                     /translation="MINIRKAQKNDLENLLLLYTHLNNNQMPIIDNNVEQIWDDILSD
                     PNHHIIVGTIGEKIVSSCVIVIVKNLTHNQRPYALIENVVTCEEHRGRGYATEILNYA
                     KEIAKRENCYKIMLMTGSKKESTLNFYKRAGYNDKDKTAFIQWIDK"
     gene            643..918
                     /locus_tag="PLA01_08580"
     CDS             643..918
                     /locus_tag="PLA01_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01387.1"
                     /translation="MYFSSKKGLGIGLLIWIFMIAGLIATFSAALFTVYRTKLWFIMV
                     IYIVPLFFAGWIWFGTGYFISPKEKEKFIDELNNEIKKCNGSGVSLL"
     gene            complement(1005..2645)
                     /gene="gltX"
                     /locus_tag="PLA01_08585"
     CDS             complement(1005..2645)
                     /gene="gltX"
                     /locus_tag="PLA01_08585"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPD01388.1"
                     /translation="MDYKKLADMLFPNITKSISYYEENVFPDRDLPDGAKVTRLAPSP
                     TGFIHLGNLYGAFVDERLAHQSNGVFILRIEDTDEKRKVEGAVETIITSLEFFDLRFD
                     EGAGIDGETGNYGPYFQSNRGEIYQTVAKRLIEMGRAYPCFCSEEELGKIREQQIAEN
                     VNTGYYGKWAVDRDLSLEEVKKHLDNSDSFVIRFKSMGNPEDTFEIDDAIRGRITSPV
                     NFQDIVILKANGIPTYHFAHVVDDHLMKVTHVVRGEEWLSTLPIHYELFTTLGWDLPV
                     YCHTAHLMKIDNGVKRKLSKRKDPELGLEYYMQLGYHPAAVREYLMTILNSNFEEWRI
                     QNPDSDIEDFPFTLNKMSNSGALFDLNKLNDVSKNVLVKIPATEIYEFLLNWAKDYKN
                     EIVSLLIEHKDAIIKLLDVGRNSDKPRKDLIYCEQIFDFISYYFDGYFKIVDEFPENI
                     DDVEAKKLLKAYLETYNHSDDQNLWFEKIREIASQNGYAAKPKDYKKNPEMYKGHVGD
                     VSTVIRIAVMGRSTSPDIWELQQIMGEEKVRKRIQSLI"
BASE COUNT          808 a          508 c          515 g          891 t
ORIGIN      
        1 ttggaattat attacttcat gcgatagccc tgtatatgca aataaacttg attgaataag
       61 gttaatatga tttttgaccg gagtggacag catttgtatg tatatcctgg atgccttgca
      121 gaatatcgaa aaaaacacta aatatggatg aattgggggt tgagaaatga ttaacattag
      181 gaaagcacaa aaaaatgacc tggagaattt acttctgtta tatacccatt taaataataa
      241 tcaaatgcca ataatcgata ataatgtgga acaaatttgg gatgacattt tgtccgatcc
      301 aaaccatcat attattgtcg gcactattgg agagaaaatc gtctcatctt gtgtgattgt
      361 aattgttaag aacttaaccc ataatcaacg gccttatgca ttaattgaaa atgttgttac
      421 atgcgaagaa cataggggaa gagggtacgc aacagaaata ttgaattatg caaaggaaat
      481 tgcaaagcgg gaaaattgct ataaaatcat gttgatgaca ggatccaaaa aagaatccac
      541 cctgaatttt tacaaaagag caggttacaa tgataaagac aaaactgcat ttattcaatg
      601 gattgataaa taaactgttc actaaaggag gcagtactca atatgtattt ttcatcgaag
      661 aagggcctgg gtatcggtct tctgatttgg atatttatga tagcaggatt aattgctact
      721 tttagtgctg ctttatttac tgtatatcgc actaaactat ggtttattat ggtgatttat
      781 attgttcctc tcttttttgc cggatggatc tggtttggaa ccggttattt catatcacca
      841 aaagaaaaag aaaaatttat tgatgaattg aataatgaaa taaaaaaatg caatgggtcc
      901 ggtgtaagtt tattatgaga tagcgatatg tgcaagatat ttatattttc gaatgcaaaa
      961 aaactaaggg acttataatc ccttagtttt tttataagaa aaatttatat aaggctttga
     1021 attctttttc tgactttttc ttcacccata atttgctgaa gctcccatat atccggggaa
     1081 gtgctgcggc ccataacggc aatccttata acagtactca catcgccaac atggccctta
     1141 tacatctccg gattcttctt atagtctttt ggctttgctg catatccgtt ttggcttgct
     1201 atttcccgga ttttttcaaa ccacaggttt tgatcgtcac tatggttgta ggtttccagg
     1261 tatgccttaa gcagcttttt agcctcaacg tcatctatat tttcagggaa ttcatcgaca
     1321 attttaaaat aaccgtcaaa gtagtagctg ataaaatcaa atatctgttc acaatatatt
     1381 aaatccttac ggggcttatc ggaatttctt ccaacatcga gaagttttat tattgcatcc
     1441 ttgtgctcaa taagaagact gactatttca tttttataat ccttggccca attaagcagg
     1501 aattcatata tttctgttgc cggaatttta actaaaacgt ttttacttac atcgttgagc
     1561 ttgttaagat cgaacagggc tccggagtta ctcattttgt ttaaggtaaa aggaaaatct
     1621 tctatatcgc tgtccgggtt ttgaattctc cattcttcaa agtttgagtt cagaatggtc
     1681 atcaggtact cccgtactgc tgccggatgg taacccagtt gcatgtaata ctccaatccc
     1741 aattccggat cttttctttt tgaaagcttt cttttaactc cgttgtctat cttcattaaa
     1801 tgagctgtgt ggcagtatac cggcaaatcc caaccaagag tggtaaacaa ctcataatgt
     1861 atcggcaggg ttgaaagcca ttcttcacct cttacaacgt gggtaacttt catcagatgg
     1921 tcatccacca catgggcaaa atgatacgtg ggtattccgt tggctttaag aataactata
     1981 tcctgaaagt taaccggtga ggtaatacgg ccccttattg catcatcgat ttcgaaggta
     2041 tcctccgggt tgcccatgga tttgaaacgt ataacgaagc tgtcgctatt gtcaaggtgc
     2101 tttttgactt cttcgaggga caaatccctg tcaacagccc acttgccgta gtatccggtg
     2161 ttaacgtttt ctgcaatctg ctgctctctt attttgccaa gctcttcctc ggaacagaag
     2221 caaggataag ctctgcccat ttcaatcaat cgttttgcaa cggtttgata gatttctccc
     2281 cggttgctct ggaaataagg tccgtaatta ccggtttctc cgtcaatacc tgcaccctcg
     2341 tcaaatctta ggtcaaagaa ctctaaagag gttatgatgg tttcaactgc tccctcaacc
     2401 ttacgctttt catcggtatc ctcaattcgg agaataaata cgccgttgct ttgatgggcc
     2461 aggcgttcat cgacaaaagc tccataaaga ttcccaagat gaataaagcc tgtgggactt
     2521 ggtgcaagac gggtgacttt tgctccgtca ggtaaatctc tgtcgggaaa tacgttttcc
     2581 tcgtaataag atattgactt tgttatgttt ggaaaaagca tgtcagctaa ctttttgtaa
     2641 tccataatgt acctcttttt caatttatta aatagagtag agaaataatt gctaagaatt
     2701 atttccctct cgttgaaccg ta
//
LOCUS       DAOOJC010000333         2714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_29114_length_2714_cov_3.728469, whole genome shotgun sequence.
ACCESSION   DAOOJC010000333 DAOOJC010000000
VERSION     DAOOJC010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29114_length_2714_cov_3.728469"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLA01_08590"
     CDS             <1..576
                     /locus_tag="PLA01_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HPD01389.1"
                     /translation="PGFITDIFKLGYAEFWWGVIFFALIAIILIRSKLDKSLIITSLV
                     SSAGYIGILTAYRNVVRDDNYLKDKNIQTVIWACLLISIVILLIGVYNYDKKLMLVLS
                     GLIFQYIPWIAVPRIAFIYHYFSIVPFIILLIVYVIKKAVDKYKGAKYFAYVYLGIVL
                     ALFVMFYPGLSGLEVPVSYLKILKWFPTWYF"
     gene            601..1572
                     /locus_tag="PLA01_08595"
     CDS             601..1572
                     /locus_tag="PLA01_08595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD01390.1"
                     /translation="MSNDIVCSVVVPLYNEEEVILETYKRLKGVMDSINEPYEIIFVN
                     DGSRDKTAPMVYELCSKDKTVKFVDFARNFGHQTAITAGMDYSEGQAVVVIDADLQDP
                     PELIPKMIEKWREGYDVVYGKRKERKGETLFKKFTAKVFYRFLRSMTDVDIPVDTGDF
                     RLIDRKVCEALKLVNERNRYIRGIISWLGFKQTGIEFVRDKRFAGTTKYPLKKMMKFA
                     FDAITSFSYKPLKFASVFGMLLSSISFIYLIVVICTRIFTSHVQPGWASILAVNLFFH
                     GITLIILGIVGEYIGRIYDEAKGRPLYIVRQTKNFSDDKQNKITGRM"
     gene            complement(1740..1824)
                     /locus_tag="PLA01_08600"
     tRNA            complement(1740..1824)
                     /locus_tag="PLA01_08600"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1788..1790),aa:Tyr,seq:gta)
     gene            complement(2057..2132)
                     /locus_tag="PLA01_08605"
     tRNA            complement(2057..2132)
                     /locus_tag="PLA01_08605"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2097..2099),aa:Thr,seq:tgt)
     gene            complement(2447..>2714)
                     /locus_tag="PLA01_08610"
     CDS             complement(2447..>2714)
                     /locus_tag="PLA01_08610"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626718.1"
                     /note="involved in lysine biosynthesis; DAP epimerase;
                     produces DL-diaminopimelate from LL-diaminopimelate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPD01391.1"
                     /translation="AKVLSRSEAEILIWERGAGFTLASGSSSCGVASVLRKRGLIDND
                     VKIRMLGGEIKIRIDDNWNIRMTGEVRQIAEGILSSEFLEDLNL"
BASE COUNT          837 a          499 c          508 g          870 t
ORIGIN      
        1 cccggcttta taaccgatat tttcaaatta ggatatgcag aattctggtg gggcgtaatt
       61 ttctttgcat tgattgctat tatattgatt cgctcaaagc ttgataaaag ccttattatt
      121 acctcacttg tatcctctgc cgggtatatt gggatattaa ccgcctatag gaatgtggta
      181 agagacgata actaccttaa ggataaaaac attcagacgg ttatatgggc ttgccttctg
      241 atttcaatcg ttatactgtt gataggcgtg tataactacg ataaaaaact gatgctagta
      301 ctatccgggc ttatattcca gtatatcccc tggatagctg ttccgagaat agcttttatt
      361 tatcattatt tctcaattgt accctttata atactgctga tagtatatgt aattaaaaaa
      421 gctgtcgata aatataaggg agctaaatat tttgcatacg tatacctggg aattgtattg
      481 gcactgttcg taatgtttta tcccggttta tcagggcttg aagttccggt atcctatttg
      541 aaaatcctca agtggttccc cacttggtat ttctaaaata ttagtttgga ggaaagaaat
      601 atgtctaacg atatcgtatg ttcagttgta gtacctcttt acaatgaaga ggaagttatt
      661 cttgaaacct ataagaggct taagggggtt atggattcaa taaacgaacc ctacgaaata
      721 atatttgtca atgatggaag cagagataaa acagctccta tggtctatga gctgtgcagc
      781 aaggataaaa ccgttaagtt tgtggatttc gcaagaaatt tcggtcatca gaccgcaata
      841 acagccggga tggattattc cgaaggacag gcagttgtcg ttattgatgc agaccttcag
      901 gatccccctg aacttatccc gaaaatgatt gaaaaatggc gggagggtta tgatgttgta
      961 tatggaaaaa gaaaagaacg aaaaggtgaa acattattta agaaatttac cgctaaagtt
     1021 ttttatcgct tcctgcggag tatgaccgat gtggatatac ctgtagacac cggtgatttc
     1081 agacttatcg acagaaaagt ctgtgaagct ctcaaactgg ttaatgaacg caacaggtat
     1141 attcgtggta taatcagctg gcttggcttt aaacagacag gaattgagtt tgttagggat
     1201 aaacgctttg ccggtacaac aaagtacccg ttaaaaaaaa tgatgaagtt tgctttcgat
     1261 gccataactt cattctcata caagccttta aagtttgcat ccgtttttgg gatgttgctt
     1321 tcatccatta gttttatata ccttatagtc gtcatatgta caaggatttt tacgagtcat
     1381 gtgcagccgg gttgggcgtc tatacttgcc gttaatctct tctttcacgg tataacattg
     1441 attattctcg gtatagtagg agaatacata ggaaggatat atgacgaggc taaaggaagg
     1501 cccttataca tcgtaagaca gacaaaaaat ttctcggatg ataaacaaaa caaaataaca
     1561 ggaagaatgt aaacagaaaa ctcagggaca tgttgatatg ctccccttat ggcagacagt
     1621 gaaaatataa aactgtttgc tgtaaagagg agcatatttt ataggacgaa aaagctgaat
     1681 ttcaactgat gaaaaatcca tgcaaaaagc gagtatatgc acatactcgc ttttaaattt
     1741 ggtggaggga gatggattcg aaccatcgaa ggcggagcca acagatttac agtctgcccc
     1801 ctttggccac tcgggaaccc ctccatgtaa ttcatattat ttttgacttg cattaaacat
     1861 tatacttgct ttaggctaat aagtcaataa ttttttgaag tttttaattg ccattttagt
     1921 cgatgaaacc ctattaataa aagtaaggaa agctattata aataacttta tttccatcgc
     1981 tttttgagca tttcactgcg tgaaatccta ctaaaaagtc aggtacttcg aacaactcga
     2041 agtacttgac cataattgga gctggtggac ggaatcgaac ccccgacctg ctgattacaa
     2101 gtcagctgct ctacctgctg agctacacca gcttatatta cagtataaac tgttcatttt
     2161 attggtgtct gagctgttca tttgcccttg acactatgat agtataacag cagaataact
     2221 atgtgtcaac ccattttgtg aagaaaattt gtccagtttt ttttaccacc aaagtcagta
     2281 gcaaatggct gtctgtgagc ctttaataac tcatattgac cactacattt gaatttagct
     2341 tgtgcatagc aaaatccttg taaagtgctt cacagttaac tttcacaaat gcctatgtac
     2401 aaaataccct gataattgaa gggttttata aaccatttac ttttgttcaa aggttcaaat
     2461 cttctaaaaa ctcactactt agaattccct cagctatctg tctgacttca cctgtcattc
     2521 ttatgttcca attgtcatca attcttattt ttatttctcc tcctaacatt ctaattttta
     2581 catcattatc aattaaacct cttttcctta agacacttgc tactccacaa gatgagcttc
     2641 ctgatgccaa ggtaaaacca gcacctcttt cccatattaa gatttcagct tcacttcttg
     2701 ataaaacttt tgca
//
LOCUS       DAOOJC010000334         2712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_29143_length_2712_cov_3.781332, whole genome shotgun sequence.
ACCESSION   DAOOJC010000334 DAOOJC010000000
VERSION     DAOOJC010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29143_length_2712_cov_3.781332"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1973)
                     /locus_tag="PLA01_08615"
     CDS             complement(120..1973)
                     /locus_tag="PLA01_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HPD01392.1"
                     /translation="MKRKIISYGLIVIDVILINVSLALAYFLRFDFNFEKIPEQVSEN
                     IIKFAIVSTIIKICVFMAFKLYNSIWKYVGIYETAMIMLSSVLGNLLMITYVYMVRMP
                     VPRGVFLICLLTDIFLIAGVRFAYRLIRRISKGEVFTFRNYKRVLIIGGGDAGSIVVK
                     ELRSHPELKSTPVAILDDDEMKIGKKLSGVPIVGSTKEVLNVVEKRQIDEVIIAMPSA
                     TRKKINDIYSGCSKTSCKVKILPSVSQLIDESVVMQKVRDVDIEDLLGREPVNLDIEE
                     VSSYLKNQVVMVTGGGGSIGSELCRQIAGFSPKCLIILDNYENNAYEIQNELLYKHPE
                     LKLETIIANIREKQRLENIFRQYKPDVVFHAAAHKHVPLMEANPTEAIKNNVFGTLNV
                     AKCADEFGTKRFVLISTDKAVNPTNIMGATKRIAEMIIQSLNRVSKTEYVAVRFGNVL
                     GSNGSVIPLFKKQIAQGGPVTVTHPEVTRFFMTIPEAVQLVIEAGAMAKGGEIFVLDM
                     GEPVKISDLARNLIKLSGFEPDVDIKIKYTGLRPGEKLYEELLLNEEGLQATKNNKIF
                     VAKPIHIDMAMLIRELDYLKEVMATNSEGISEYIKLIVPTYKKAENSGISS"
     gene            complement(2173..>2712)
                     /locus_tag="PLA01_08620"
     CDS             complement(2173..>2712)
                     /locus_tag="PLA01_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HPD01393.1"
                     /translation="KEATIINERNMNPFVFGAATLETIEKMANLNSNIGVEAFNQINS
                     QKFLSVNEIDINSDGIPDQWKNLTPNNAEVGVENGLIVAKKIDKSGAGAMQTRPIALE
                     EGKKYKLELVLDNDVKEVSYQVINYHKKYQKLEKIGAGVFSAEIEMTSEEINNEEYSV
                     RLRIDKDLVIKSLTVSQVP"
BASE COUNT          761 a          613 c          428 g          910 t
ORIGIN      
        1 gacatttgat acagaacata atcagatttt aatattaatt taggggaaat aatgaaaaaa
       61 aggggcttta atgccccttt agtttttgtt tatgaagact tgatgccctc tttttttgtc
      121 taagatgaaa ttccgctgtt ttctgccttt ttataagtcg gaacaatgag cttgatatat
      181 tcggaaattc cttccgaatt agttgccata acttctttga ggtaatccaa ttctcgaatt
      241 agcattgcca tgtctatatg aatcggcttt gccacaaata tcttattatt tttcgtggcc
      301 tgcagccctt cttcattcag caataactct tcatatagct tttccccagg tctcaacccg
      361 gtatatttta tttttatgtc cacatccggt tcaaatccag aaagcttaat cagatttctg
      421 gcaagatcgg aaatcttcac cggttcaccc atgtcaagca caaatatttc tccgcccttg
      481 gccattgcac cagcctctat caccaattga acagcctcag gaattgtcat aaagaaccgt
      541 gttacctcag gatgtgttac cgtaaccggt ccgccctgtg caatctgctt tttaaacagt
      601 ggaatcacac tgccgttgct tccaaggaca ttaccgaacc ggactgccac atactcggtt
      661 ttgctgaccc tgtttaaaga ctgtattatc atttcagcaa ttctctttgt agcacccatt
      721 atattggtgg gatttacagc cttatcggta gagataagta caaacctttt tgtcccgaat
      781 tcatcagcac attttgcaac gttcaacgtt ccaaatacat tattcttaat agcctctgta
      841 ggattagcct ccatgagcgg aacatgcttg tgagccgctg catggaaaac aacatcgggc
      901 ttatactgtc tgaatatatt ttccagtctt tgcttttctc ttatattggc aattatggtc
      961 tccagcttca actcaggatg cttgtacaat aattcgttct gtatttcgta agcgttgttt
     1021 tcgtaattat ccaatataat caggcattta ggagaaaaac ctgctatctg cctgcacagc
     1081 tccgatccaa ttgatcctcc gcctccggtt accataacaa cctgattctt cagataggac
     1141 gaaacctctt ctatgtccag attcaccggt tcccttccta aaaggtcttc aatgtcaaca
     1201 tccctgactt tctgcataac aacagactcg tcaataagct gtgacaccga cggaagtatt
     1261 tttaccttgc agctggtttt tgaacaaccg ctgtatatat catttatttt tttacgggta
     1321 gcagaaggca tcgcaattat tacctcgtct atctgccttt tctctaccac attgagaacc
     1381 tctttcgtac ttccaacgat aggaacgccg ctcagcttct ttcctatctt catttcgtca
     1441 tcatcaagta ttgcgacagg agtacttttg agctccggat gcgagcgcag ctcttttaca
     1501 actattgaac ctgcatctcc gccgccaata atgaggaccc gtttataatt cctgaatgta
     1561 aaaacttcgc ctttagaaat cctcctaatc agcctgtatg caaatcttac tccggcaatc
     1621 aagaaaatat ccgtcaacaa gcatatcagg aatactcccc ttggcacagg catccgtacc
     1681 atataaacat aagttatcat taagagattt cccaatacgg atgacagcat tatcatagcc
     1741 gtctcgtata ttccgacata cttccatatg ctgttataaa gtttaaaggc cataaataca
     1801 caaatcttga taattgtaga tacaatagca aactttatta tattctctga aacttgttct
     1861 gggatttttt cgaaattaaa atcgaatctt aaaaagtatg ctagagccag tgatacatta
     1921 attaatatta catcgataac gattaagccg tatgagatga ttttgcgttt cataatacct
     1981 ccccgaatta ccccattcta agaatttttt ttatttatgt tagatcctct acgactataa
     2041 taccataatc agttaaatat ttaaagagta atttttctac ataattttgt ttaatattac
     2101 acatttttta gtattaatat attacaaatt tggcgtagaa tataccgatt gaagtaaaca
     2161 tattgtttga aatcagggaa cttgtgaaac cgtaaggctt ttaataacca agtccttgtc
     2221 tatgcgaagc ctcaccgaat attcttcatt gtttatttct tccgatgtca tttctatttc
     2281 tgccgaaaat acaccggcac cgatcttttc aagtttctga tactttttat ggtaattaat
     2341 tacctggtac gacacctctt taacatcatt atccaaaacc aattccagtt tatacttttt
     2401 cccttcctca agggcaatgg gccgggtctg cattgctccg gcaccggatt tatcgatttt
     2461 cttcgcaacg ataagcccat tttcgacccc gacctccgca ttattcggag ttagattctt
     2521 ccactggtcg ggaataccat cggagtttat gtcaatttca ttgacactaa gaaacttctg
     2581 gctatttatt tgattaaagg cttcaactcc aatattgcta ttcaaattag ccattttttc
     2641 aatagtttcc aaggttgcag caccgaacac aaaaggattc atatttcttt catttataat
     2701 agtagcctcc tt
//
LOCUS       DAOOJC010000335         2705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_29252_length_2705_cov_3.573208, whole genome shotgun sequence.
ACCESSION   DAOOJC010000335 DAOOJC010000000
VERSION     DAOOJC010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29252_length_2705_cov_3.573208"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /gene="rimO"
                     /locus_tag="PLA01_08625"
     CDS             complement(<1..1119)
                     /gene="rimO"
                     /locus_tag="PLA01_08625"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPD01394.1"
                     /translation="MNKKIGVVSLGCPKNLVDSEIILGLLKKKDFEITADKEEANVII
                     VNTCGFIESAKEESINTILEMANYKKGNCELLVVMGCLAQMYKDEIKKEIPEVDFVVG
                     VSGYGEIARIIDKAYNNGVESISSKAMYCDNSCGIEYLSNDRLISTNKGYAYLKIAEG
                     CNNCCTYCVIPSIRGPYRSRSMEDIVGEAQSLAKNGIKEVILVAQDVTVYGRDLYGEK
                     KLVDLIRAISGIQGIEWIRLLYCYPEEIDDALITEIADNRKVVKYLDIPIQHASDKIL
                     KMMGRRGNSEAIRNTLVKLRSSIPRLVLRTSLIVGFPGEDEKDFKILYDFVREFEFER
                     LGVFTYSREEGTPAYDLKPQIKKSVKDSRRSDIMQIQKE"
     gene            complement(1182..1433)
                     /locus_tag="PLA01_08630"
     CDS             complement(1182..1433)
                     /locus_tag="PLA01_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01395.1"
                     /translation="MSDYDKRLNQIKESLDKANNLRIRAEAKLEQLSRQKEDILSELK
                     EMGVEPENLDSEIRKLKDDIEDLITKAEKLIPEELIKNN"
     gene            complement(1433..>2705)
                     /locus_tag="PLA01_08635"
     CDS             complement(1433..>2705)
                     /locus_tag="PLA01_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPD01396.1"
                     /translation="GTKSVRVELELSNGYTITRERTPGKNRYVLQDPEGNASIFEGFG
                     NEVPLEIIKAHGIPKVVLDTDVSSCINIGEQLEGPFMLSESGSTRAKAIGRLTGIHII
                     DRSIRDCVTDIRRENQTRDRVSSDIGDVDERLKGYSEIIELGKKLDKAQEFIVRMEAS
                     MERANTLLDKKQHLGVIESEYSKTERILAGLDKLNECDVYIKSAELSLHKIESLEGIK
                     KKYFDVTGRINEMEKVMKQTAGVSEGREILEEIFERSLKFDRLKKIQAELTKVNSELG
                     RAKGILEGTKDIKNLDMLIKRVSDSVLIESKIAPAAERLLSIEREIKRLKAHISSFEN
                     VKYSQNLILLAEQKLENLKRLETLNKDYKEKTDSIREGMRFLEEKKTEISQLLKNYTQ
                     ELREKNVCPLCGSDIKEEKLENIIRHYEEVH"
BASE COUNT          680 a          680 c          391 g          954 t
ORIGIN      
        1 ttccttctgt atctgcatta tatcacttct tcttgaatcc ttaacacttt ttttgatttg
       61 cggctttaaa tcatacgcag gtgtcccctc ttccctcgaa taggtaaaca caccaagcct
      121 ttcaaactca aattcccgta caaaatcata aagaatttta aagtcctttt catcctctcc
      181 gggaaagccc actattaaag aggttcttaa tacaaggcgg ggtattgaag acctgagctt
      241 taccaatgta ttcctgatgg cttcactgtt tcctcttcta cccatcatct taagtatctt
      301 atcgcttgca tgttgaatgg ggatgtccaa atattttaca accttgcggt tatccgcaat
      361 ttctgttatc aatgcatcgt caatttcctc aggatagcag taaagaagcc taatccactc
      421 aatcccttga ataccgctga ttgctcttat taaatccacc agtttttttt cgccatagag
      481 atccctgcca tacaccgtta catcctgggc caccagaata acttccttta tcccgttttt
      541 agccagggac tgtgcctcac caacaatatc ttccatgctt ctgcttctat agggaccgcg
      601 tatggaagga ataacacagt aagtacagca attattgcat ccttcggcta tttttaagta
      661 tgcatatcct ttatttgtag aaatcaggcg gtcatttgat agatactcga taccgcagct
      721 attatcacag tacatagcct tggaagaaat actctcaacg ccattattgt aagccttgtc
      781 aataatcctg gctatctcgc cgtaacctga aactccaaca acaaaatcaa cctcaggtat
      841 ctcctttttt atctcatcct tgtacatttg agcaaggcat cccataacca ctaatagctc
      901 acaattgcct tttttataat tggccatttc gagaatggta tttattgatt cttcctttgc
      961 agattcaata aaaccacatg tatttactat aatgacattg gcttcttctt tatcggctgt
     1021 aatttcaaaa tcctttttct ttagtaatcc cagtattatt tcactatcaa caagattttt
     1081 ggggcatccc agtgaaacaa cacctatttt cttattcaaa atatacacct catcaaagct
     1141 tattatattc aattacatta ttcattgtta aaaagaaaat gtcaattgtt ttttattagt
     1201 tcttctggta ttaatttttc tgcttttgtt ataaggtctt caatgtcgtc cttaagtttt
     1261 cttatttcgc tgtcgagatt ttccggttct acccccattt ccttaagctc tgaaagaata
     1321 tcctcctttt gcctgctaag ctgctccagc tttgcctcag cccgtattct aaggttattt
     1381 gccttgtcca ggctctcttt tatctgattg agcctcttgt catagtcaga catcaatgca
     1441 cctcctcata atgccttata atattctcaa gcttttcctc ttttatgtca cttccgcata
     1501 acggacagac attcttctcc cttaactctt gggtatagtt cttgagtaat tggctgattt
     1561 ccgtcttttt ttcctcaaga aacctcattc cctcccttat actgtcggtc ttttctttat
     1621 agtctttatt aagagtctcc agcctcttta aattctcaag cttctgctcg gccaagagta
     1681 taaggttttg cgagtatttc acgttttcga aggaagatat atgggcctta agcctcttaa
     1741 tctccctttc aatagacaat aacctctctg cagccggagc gatctttgac tcgattaaca
     1801 cactgtcact gactcttttt attaacatat cgaggttttt aatatccttt gttccttcca
     1861 gtataccctt tgccctgccc agttcactgt taaccttcgt aagctctgcc tgaatctttt
     1921 ttagcctgtc aaatttcaag gatcgctcaa atatctcctc taaaatctct cttccttcac
     1981 tcaccccggc agtctgcttc atcacttttt ccatttcatt aattcttccg gttacatcaa
     2041 agtatttttt cttaatgccc tcgagagatt caattttatg aagggacagc tcggcacttt
     2101 ttatatatac atcacattca ttcagcttat ccagccctgc caatatcctc tctgttttag
     2161 agtattcact ttcaatgact cccaaatgct gttttttatc caatagagta ttagccctct
     2221 ccatagaagc ttccatccgg acaatgaact cctgtgcctt atcaagtttt ttgccgagtt
     2281 caattatctc actataacct ttaagccttt catcaacatc gcctatatca ctgctgaccc
     2341 ggtcacgggt ctggttttcc cttcgtatat cggtaacaca atcccttatt gacctgtcaa
     2401 taatatgtat tcccgtcagc cgtcctattg cttttgcccg tgtagaaccg gattccgaca
     2461 acatgaaagg accctcaagc tgttctccaa tattgatgca ggaactgaca tccgtatcca
     2521 aaaccacctt tggtattcca tgggctttaa taatttccaa aggcacttca tttccaaaac
     2581 cttcgaatat actcgcattt ccctccgggt cttgaaggac ataccggttt ttgcccggag
     2641 ttctttccct ggtaatggta taaccattgc tcagttcaag ctccacccta acagattttg
     2701 taccc
//
LOCUS       DAOOJC010000336         2697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_29385_length_2697_cov_3.592354, whole genome shotgun sequence.
ACCESSION   DAOOJC010000336 DAOOJC010000000
VERSION     DAOOJC010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29385_length_2697_cov_3.592354"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLA01_08640"
     CDS             complement(<1..183)
                     /locus_tag="PLA01_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPD01397.1"
                     /translation="MGKNKKNKNDGKMKTIMLVAAIAVVVLALGGYIWYSSITSYVVT
                     IAGEKVSSADYKYFLST"
     gene            complement(350..>2697)
                     /gene="mfd"
                     /locus_tag="PLA01_08645"
     CDS             complement(350..>2697)
                     /gene="mfd"
                     /locus_tag="PLA01_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPD01398.1"
                     /translation="SYQGHLDFLMEDIKHWKEKRSRVVILSGTKGRGEMLTEALASRN
                     IEAVFLEELSRDVEPGEIVVTHGTLHTSFEYSDIGFVVVSGKELFGQDRKARRLKSNK
                     NKGQKISVFTDLNVGDYVVHRLHGIGKYTGIEQLVVENVKKDYLKIQYQEGDFLYVPT
                     NQLDLIQKYIGSEGKTPRLSKLGGSDWIKTRTRTKKALMELAGELIKLYAEREALKGY
                     SFGKDTVWQKQFEELFPYQETDDQLRCIEEIKADMESSKPMDRLLCGDVGFGKTEVAI
                     RAIFKAIMDGKQVAYLVPTTVLAQQHYNNFKERMKDFPDTVEMISRFRTKAEQKKILK
                     DVKTGVVDVLVGTHRLLQKDVGFKDLGLLVIDEEQRFGVQHKERIKKMKANVDVLTLT
                     ATPIPRTLHMSLTGIRDISTIEDPPEERYPVQTYVMEYNPEVIKDAINREISRGGQVF
                     YLYNRVRSMDIKAVTVQKLVPDARIGIAHGQMNETELEDVMLRFINGEYDVLVCTTII
                     ESGLDMPNVNTIVVENADTMGLAQLYQLRGRVGRSNRLAYAYITYKKDKVLSEIAQKR
                     LQAIKEFTEFGSGFKIAMRDLQIRGAGNILGPQQHGHIDSVGYDMYCRLLAEAVNELQ
                     GVPVADADLEISIDINISAYIDDEYIEDENQKIEIYKKIASINNEQDVLDVEDELMDR
                     YGEIPQSVRNLLEIALIKSLARTCGFLSVQEKNGTVMFQYAQNSNINFEALGRLMDKY
                     KRKLLFTASHNPYITYKITDIKRSELLDNIKILLQDINKLQ"
BASE COUNT          693 a          684 c          423 g          897 t
ORIGIN      
        1 cgtagataga aaatacttat aatcggcact ggagaccttt tctcccgcaa tggtgactac
       61 atagctcgta atactggaat accatatata gcctcccaaa gccagcacaa ccactgctat
      121 cgcagctact aacataatag ttttcatctt gccgtcattt ttattttttt tgtttttacc
      181 caataaagat ccctcacatt cactaagtaa tatggactat tttctaaaat ccccttattt
      241 ccgggatctt ataaatagtt ctaaaaaagt acataaattg ttttcatttt tggggttata
      301 taatctacaa aataacccca actatataca ttataataaa ttgtacactt tactgcaatt
      361 tattaatgtc ctgtaacaaa attttaatat tgtccagtaa ttcgcttctt tttatatcgg
      421 taattttgta tgtgatgtaa ggattgtggc ttgctgtaaa aagaagcttt cttttgtatt
      481 tatccataag cctccccaat gcctcaaagt taatattact gttttgggca tactggaaca
      541 ttacagtacc gttcttctcc tgcaccgaca aaaatccgca tgtccttgcc aaagacttaa
      601 ttaatgcgat ctccagtaaa ttccttacag attgcggtat ttcgccatag cggtccataa
      661 gctcatcctc tacatccaaa acgtcctgct cattattaat cgatgcaatt tttttgtata
      721 tttctatctt ctgattttca tcctctatat actcgtcatc tatgtaagca cttatgttaa
      781 tatctataga tatctcaaga tcggcgtcag caacaggaac tccttgaagt tcgtttaccg
      841 cctcggcaag aagcctgcag tacatgtcat aacccacgga atctatgtgt ccgtgctgct
      901 gggggcctaa tatatttccc gctcccctta tctgcaagtc ccgcattgcg attttaaaac
      961 cggatccgaa ctccgtaaac tcctttattg cctgaagcct tttctgtgca atctccgata
     1021 gtaccttgtc ctttttatag gttatgtatg cgtaggcaag cctattggaa cgcccaactc
     1081 tgccccttaa ctggtaaagc tgtgcaagtc ccatggtatc tgcattttca actacaatag
     1141 tgtttacatt cggcatatca agaccggatt caattattgt agtacaaaca agaacatcat
     1201 attccccatt gataaaccgt aacatcacat cctcaagctc cgtttcattc atctgtccat
     1261 gggctatacc gattctagca tcgggaacca gtttttgtac cgtcacggcc tttatatcca
     1321 tggacctgac cctgttgtaa aggtaaaaga cctgtcctcc cctgcttatt tccctgttta
     1381 ttgcgtcttt tataacttcc ggattatatt ccataacata tgtctgaaca gggtatcttt
     1441 cctccggagg gtcctcaatg gtgcttatat ccctgatgcc tgtaagcgac atgtgaagtg
     1501 ttcttggaat cggtgttgca gttagggtaa gaacatccac attcgccttc attttcttta
     1561 ttctttcctt atgttgtacg ccaaacctct gctcttcatc tattaccaaa aggcccagat
     1621 ccttaaaacc aacatccttc tgaagcaagc ggtgggttcc cacaagaaca tccaccacac
     1681 cggtcttaac atcctttaat attttctttt gttctgcctt tgttctaaac ctgctgatca
     1741 tctcaacagt atcaggaaaa tccttcattc tttccttgaa gttgttgtaa tgctgctggg
     1801 ctaaaactgt ggtgggaacc agatacgcca cctgttttcc atccattatt gccttgaata
     1861 tcgctcttat ggccacctcg gtttttccga agccaacatc gccacacaaa agcctgtcca
     1921 ttggttttga ggattccata tctgctttta tctcctcaat acacctaagc tggtcatcgg
     1981 tttcctgata tgggaaaagc tcctcaaact gcttctgcca cacagtatcc ttaccaaagg
     2041 aatacccctt taaagcttct ctttcggcat agagttttat aagctccccg gcaagctcca
     2101 ttaacgcttt cttggttctt gttcttgttt ttatccaatc gcttccccca agcttgctga
     2161 gccttggcgt ttttccctca gagcctatat atttttgtat caaatccagc tggtttgtag
     2221 gaacatataa aaaatccccc tcctggtatt gaatcttcag ataatccttc ttaacgttct
     2281 ccactaccag ctgttcaatg cctgtatatt ttccgattcc atgcaaccgg tgtacgacat
     2341 aatcgcccac attcaagtcc gtaaaaacac ttatcttctg ccctttgttt ttattggact
     2401 ttaatctcct tgcttttctg tcctgcccga aaagctcttt accgctcacc accacaaacc
     2461 cgatatccga atattcaaaa ctcgtgtgca aggtaccgtg ggttactact atctctcccg
     2521 gttccacatc acggcttaat tcctctaaaa aaacagcctc tatattcctg cttgccaaag
     2581 cttccgtcag catttcccca cggccttttg taccggaaag tataacaacc cttgagcgtt
     2641 tttccttcca atgctttata tcctccatca aaaagtccag atgtccctgg tatgagt
//
LOCUS       DAOOJC010000337         2693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_29453_length_2693_cov_3.714556, whole genome shotgun sequence.
ACCESSION   DAOOJC010000337 DAOOJC010000000
VERSION     DAOOJC010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29453_length_2693_cov_3.714556"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PLA01_08650"
     CDS             complement(<1..395)
                     /locus_tag="PLA01_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HPD01399.1"
                     /translation="MDKNILPRAWESLLNSSEFIPVIVKTIEQFHGPSWHMEPNRHES
                     FEMVYIKRGKAVFEIAGYPAQIGPNDIIIIKPNQPHKFIVKSETGCEFIVLSFKFVNS
                     FASQHSDVSLEDFLNFVSGKESGAFITLKV"
     gene            complement(440..1054)
                     /locus_tag="PLA01_08655"
     CDS             complement(440..1054)
                     /locus_tag="PLA01_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HPD01400.1"
                     /translation="MMDLLGSEIFGWVVLPILIFLSRIIDVTIGTIRIIFVSRGKKNL
                     APILGFFEVMVWVMAISQIMQNLNNFFCYFAYAAGFATGNYVGIIIEEKLAIGTLVVR
                     VIMTKDEHQLKERLADAGFGVTAVDAEGKNGKVKIIYSIIKRKELQEAVRIIETCNSK
                     TFYSVEDARNVNQGIFRDAAESGGNTGVFNLLKFQRLLGSNKKK"
     gene            1471..2424
                     /locus_tag="PLA01_08660"
     CDS             1471..2424
                     /locus_tag="PLA01_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPD01401.1"
                     /translation="MRITGLTYIQRKNHVFRNVILVLIVLVLISVLLVAVISTYVAWQ
                     LSHPERSSIPDFSSNIVPKYSDVSFKDKNGEITLKGWYFDVIGSNKTLIIAHGYGSNR
                     LPYAEDTLYLIKSFLDEGYNVLTFDFRNSGESEGDMTTVGLLETNDLLGAVEFAKKKG
                     ADKIVLLGFSMGASTSILAAAESGDVDAVIADSPFSDLSSYLDDNMYVWSGLPSFPFN
                     KTILLAVSLITGLDLEDASPQMVVHQISPRPLLLIHSKDDQKIPVENSHLIYEASDKA
                     NTVFWETSGADHVRSYKVYPEEYVKRVIDFLKAADKAEEPT"
     gene            complement(2515..2676)
                     /locus_tag="PLA01_08665"
     CDS             complement(2515..2676)
                     /locus_tag="PLA01_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HPD01402.1"
                     /translation="MAIPTMIASFFGMNVQMPFGWNVDTPYAFWIIMGISAAISVMTT
                     FILYKRDMF"
BASE COUNT          859 a          553 c          458 g          823 t
ORIGIN      
        1 accttcaagg taataaaagc acccgattcc tttccgctta caaaatttaa gaaatcctcc
       61 aaagaaacat cggaatgctg gcttgcaaaa ctattgacaa atttaaagct caagacaata
      121 aactcgcagc ctgtttcgga ctttactatg aatttgtggg gctggttggg cttgattatt
      181 ataatgtcgt taggcccaat ctgtgcagga tatcccgcaa tttcaaacac cgccttacct
      241 cttttgatat aaaccatttc aaaactctca tggcggtttg gctccatatg ccagctgggg
      301 ccatggaact gctcgatggt ttttactata accggaataa attcagatga attgagcaaa
      361 ctctcccatg ccctaggcaa aatatttttg tccattttct caccccaaaa ctcttatata
      421 agcattgaac cgtccctgct tatttctttt tgttactgcc caacagcctt tggaatttca
      481 gcaagttgaa aacacctgta ttaccgccgg attcagcagc atccctgaat ataccctggt
      541 ttacattcct tgcatcctct actgaataaa aagttttaga attgcatgtc tctattattc
      601 taactgcctc ctgcagctct ttacgcttaa taatgctata aattatttta accttgccgt
      661 tttttccttc cgcatcgact gcagtaaccc caaaacctgc atctgccaaa cgttctttca
      721 gttggtgctc atctttggtc atgatgaccc tcacaacaag cgtaccgatc gcaagttttt
      781 cttctataat tattcccaca taattacctg ttgcaaaacc ggcggcataa gcaaaataac
      841 aaaaaaaatt gttaagattc tgcattatct gacttattgc cataacccat accatcactt
      901 cgaaaaatcc cagaatgggt gccagattct tctttcccct tgatacaaat ataatcctga
      961 ttgtaccaat ggtaacatca ataattctag ataaaaatat gagtatgggc aatacaaccc
     1021 agccaaatat ttccgacccc agcaaatcca ttatattaat tggaatcatt cctctcataa
     1081 taatgtgtat atattgttgt ataattgata attatatata attttatatt aaatttgata
     1141 ttaacgtcaa ccttctattc cttccagatt aaattctgga atatatttct ccgtcaccag
     1201 cttgtaaaaa aattaataat caaacacttt actaaattgt caaataaaaa tataataaga
     1261 ataaatgaat atatagtcaa cttaaaaata gacaaatatc cagcacaata catacataat
     1321 acccggaatt tcgacataca tattttgtcc tgttacttct gtccatccgt ttattcttat
     1381 cttcttacac atgtactata taaacatgca ctaagcaaag tttaatgcat atgaataaaa
     1441 tatcaaatat atgaattgga gggaagtgtc ttgagaataa caggtttaac atacatacag
     1501 agaaaaaacc atgtatttag aaatgtcatt ttagttttga ttgtgctggt tttaatttct
     1561 gtcttgctgg tcgcagtaat ttcaacttat gtcgcctggc aattgagcca tcctgaaagg
     1621 tcaagcattc ccgatttttc gtcgaatatt gttcccaaat acagtgatgt ttcttttaaa
     1681 gacaaaaatg gagaaataac tttaaaaggc tggtattttg atgttatagg gagcaataaa
     1741 acattaatta ttgcccacgg atacggttca aacagactgc cgtatgctga agacactctg
     1801 taccttataa agagctttct cgacgaaggg tataatgttc ttacctttga tttcagaaac
     1861 agcggcgaat ccgaaggtga tatgactact gtcggtttgc ttgaaacaaa cgacctttta
     1921 ggtgcagttg aatttgccaa aaaaaagggt gccgataaaa tagtcctttt aggattctct
     1981 atgggtgctt ccacaagtat tttggctgca gccgaaagtg gggatgtcga tgcggtaata
     2041 gcagacagtc ctttttccga cttaagcagc taccttgatg ataatatgta tgtatggagc
     2101 ggtcttcctt catttccctt taacaaaacc atattgctgg cagtatctct cattacaggc
     2161 ttggatttgg aagatgcgag tcctcaaatg gtggtgcacc aaatttcacc caggccattg
     2221 cttctcattc acagcaagga tgaccagaaa atccctgttg aaaacagcca cttgatatat
     2281 gaagcctccg ataaagcaaa caccgtgttt tgggaaacca gcggtgcgga tcacgtgaga
     2341 agctacaagg tatatcctga agaatatgta aaaagagtga tagacttttt aaaagcagcg
     2401 gataaagctg aggagccgac ataagaaaca gaaataacaa acgggtaaga cttaaaaggg
     2461 ctgaatgccc ttttttaatt aacagaacga ttttttacac ttgttactgc actcttaaaa
     2521 catatctctc ttatatagaa taaaggttgt catcacggaa atagccgccg atatccccat
     2581 tataatccaa aaggcataag gagtatcgac attccaccca aaaggcatct ggacgttcat
     2641 gccgaagaaa ctggctatca tagtgggtat ggccattact atggttacag atg
//
LOCUS       DAOOJC010000338         2693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_29456_length_2693_cov_3.028810, whole genome shotgun sequence.
ACCESSION   DAOOJC010000338 DAOOJC010000000
VERSION     DAOOJC010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29456_length_2693_cov_3.028810"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1356
                     /locus_tag="PLA01_08670"
     CDS             97..1356
                     /locus_tag="PLA01_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010680970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPD01403.1"
                     /translation="MDVAKVVGYFGSVEGSSSGMLFVNACTRIAIIIVSVFVAYSSEK
                     ERKHIKRLEWLAYIDAVTGVYNHRYFQTKLEEGIEELEGTNGSLSLIMIDLDNFKKYN
                     DTNGHKAGDLLLSKTAEVILHEVGKKDIVCRYGGDKFAIITHESNPEEILLLIERIRK
                     AYNDMISSEEFDRITDNATLSVGYSIYPALARNKDELIMQSDSALYQAKNMGRNNVQL
                     YRNVFEEIKTFFSSDEHQLFGGLRALLGTVSAKDKYTLGHSERVMDYAVMIGRAMGLS
                     NENLRLLKIAALLHDIGKVEIPETVLNKTEPLTPSEIDVLRKHPSYSVDILEPLASIE
                     LLIDSIKYHHERYDGNGYPSGIKGKSIPLGARILCVADAFDAMLSDRPYRKGLRMDQV
                     ISELKKNAGTQFDPDTVNAFLTTLSVN"
     gene            1565..>2693
                     /gene="eno"
                     /locus_tag="PLA01_08675"
     CDS             1565..>2693
                     /gene="eno"
                     /locus_tag="PLA01_08675"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPD01404.1"
                     /translation="MKQYLEIESVFGREILDSRGNPTIEVEVIAEGGFIGRAAVPSGA
                     STGAFEAVELRDNDKNRYLGKGVQKAVDNVNNIIAPKVEGMNVFDQVAIDNMMISLDG
                     TPNKSKLGANAILGVSLATAKAAAEALGLSLYQYIGGVNGKTLPVPMMNIINGGKHAD
                     NSVNIQEFMIMPVGACCFKNALQMCAEVFHNLKKVLKDKGYSTAVGDEGGFAPNLKTD
                     EEAIQVILEAVEKAGYKPGDDFRIAIDAAATEMYQEDGSYLFWKSGIKKTKEEMISYW
                     EGLVNKYPIISLEDGVAEEDWEGWKMLTDRIGGKIQLVGDDLFVTNTERLKKGIDLGV
                     SNSILIKVNQIGTLTETLDAIEMANRAGYTAVVSHRSGETED"
BASE COUNT          868 a          414 c          666 g          745 t
ORIGIN      
        1 tgaccatctg gctgaacccg aagggtagca aggacaaata ccttggcatc cattcacatg
       61 ttcaggctgt caaaagtgat acacgcgtat gtatctatgg atgtagccaa agtagtgggg
      121 tattttggaa gtgtagaggg cagctcttcg ggaatgcttt ttgtaaatgc ttgcacaagg
      181 attgcgatta taattgtatc ggtttttgtt gcttactcat cggagaagga acgcaagcat
      241 ataaaaagat tggagtggct ggcttatatt gatgctgtaa cgggtgttta caaccacaga
      301 tattttcaaa cgaagctgga agagggaata gaagagcttg agggtactaa tggttctcta
      361 agcttgataa tgattgattt ggataacttt aagaaatata acgatactaa cggccacaaa
      421 gccggggatt tgttattgtc aaaaacagct gaggttattt tgcatgaggt gggaaagaaa
      481 gatattgtat gcagatatgg cggggataag ttcgctatta taacacatga gtccaatcct
      541 gaggaaatat tattactgat tgaacgtata cgaaaggctt ataatgatat gatttcttcg
      601 gaagaatttg accggataac cgataatgca actctgtccg tcggttattc catataccct
      661 gctcttgccc ggaataagga tgaattaatt atgcagtccg atagtgcact ttatcaggca
      721 aagaatatgg gaaggaataa tgttcaatta tacaggaatg tttttgaaga aattaagaca
      781 tttttcagtt ccgatgagca tcagcttttt ggtgggctaa gggctctctt agggactgtt
      841 tccgcaaagg acaagtatac gctgggccac tctgaaaggg taatggatta tgctgttatg
      901 atcggcagag ctatgggatt aagcaatgaa aatctcaggc ttcttaaaat agctgctttg
      961 cttcatgata tcggtaaggt ggagatacct gaaactgtgt tgaacaagac cgagccgcta
     1021 acaccgagcg agattgatgt tttaagaaaa catccgagct atagtgttga tatattggag
     1081 cccttggcaa gtattgagct cttgattgat tcaattaagt accaccacga gaggtacgat
     1141 ggaaacgggt atcccagtgg aattaaaggg aaaagcatac cgcttggggc cagaatttta
     1201 tgtgttgcag atgcgtttga tgcgatgtta tcggatagac cttacagaaa aggtctgaga
     1261 atggatcagg tgatatcgga gttgaaaaag aacgcaggaa cacagtttga cccggatacg
     1321 gtaaatgctt ttttgactac gcttagtgtt aattagaaac aatgaataaa agctcatgtt
     1381 accggagcac ttttctccgc agctatcgga tgtaccgggc tttgatactt gtattgtaat
     1441 tgttgaaaat atgttttaac aataataaat ctggacaaaa taaattagat atatttacaa
     1501 agtattttat gtaagatatt ctattaaggg aacaaattat atatatatga agggagaatt
     1561 atcgatgaaa caatacttag agattgaaag tgtatttgga cgggagatac ttgattccag
     1621 agggaatccg acaattgaag ttgaggttat tgcagaagga gggttcatag ggagagcagc
     1681 agtaccgtcc ggtgcatcaa caggtgcttt tgaggctgtt gaacttagag ataatgataa
     1741 aaatagatac cttggtaaag gcgtacaaaa ggctgtagac aatgtcaaca atattatagc
     1801 accaaaagtt gaaggcatga atgtgtttga tcaggttgct attgacaata tgatgataag
     1861 tcttgacggt acaccaaaca agtcgaaact tggagcaaat gctattttag gtgtatccct
     1921 tgcaacagca aaagcagctg ctgaagcatt gggattaagt ctttatcagt atattggagg
     1981 ggttaatggc aaaacacttc ctgtaccgat gatgaacata atcaacggcg gaaagcatgc
     2041 cgataacagt gtaaacattc aggagtttat gattatgccg gtaggggcat gctgctttaa
     2101 aaatgctctt caaatgtgtg cagaggtttt ccataactta aagaaagttt taaaggacaa
     2161 gggatacagc actgctgtcg gtgatgaggg aggttttgca ccaaacctta agaccgatga
     2221 agaagctatt caggttatat tggaggcagt agaaaaggcc ggatacaaac cgggagatga
     2281 cttcagaatt gctatagatg ctgctgcaac agaaatgtac caggaagacg gatcttacct
     2341 attctggaag tcaggcatta aaaagacaaa agaagaaatg attagctatt gggaaggatt
     2401 ggtaaacaaa tacccgatca tttctcttga agacggtgtt gctgaagagg attgggaagg
     2461 ctggaaaatg cttaccgata gaattggagg caagattcag cttgtaggag atgacctttt
     2521 tgttaccaat accgaaagac ttaaaaaggg tattgatctt ggggtatcaa attccatact
     2581 tataaaggtt aatcagatcg gtacattaac tgaaaccctt gatgctatcg aaatggcaaa
     2641 tcgtgcagga tataccgcag ttgtatctca caggtcggga gagactgagg atg
//
LOCUS       DAOOJC010000339         2685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_29585_length_2685_cov_2.928897, whole genome shotgun sequence.
ACCESSION   DAOOJC010000339 DAOOJC010000000
VERSION     DAOOJC010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29585_length_2685_cov_2.928897"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /locus_tag="PLA01_08680"
     CDS             <1..172
                     /locus_tag="PLA01_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPD01405.1"
                     /translation="ANTEKKVPREWINEEGNGIKQGYIDYALPLIQGESKPPIEDGLP
                     RFAKLKKVLAKK"
     regulatory      197..277
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            361..2595
                     /gene="pflB"
                     /locus_tag="PLA01_08685"
     CDS             361..2595
                     /gene="pflB"
                     /locus_tag="PLA01_08685"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HPD01406.1"
                     /translation="MIESAWRNFKPGAWQKEIDVRDFIVKNYTPYDGSEDFLVQSSDR
                     TRKLWDKCKELLREEYLKSGVLDIDTSTVSGITAYKPGYIDKDCEVIKGLQTDSPLKR
                     AVNPYGGIRMAKQACERYDYSLSPQINKIFTEFRRTHNDGVYSCYTPEMRRARKCGVI
                     TGLPDAYGRGRIIGDYRRVALYGVNRLIEGKKDDLIQLEDVDMSEETIRLREEVFEQI
                     NALQELVLMAQSYGCDITKPAEDSLEAVQWTYFAFLGAMKETNGAANSLGRVSTFLDI
                     YIDRDIKEGKITESDAQELIDQFVVKLRLIRHLRTPDYNELFAGDPVWITESIGGMCE
                     DGRHMVTKTSFRFLQTLFNLGAAPEPNITILWSERLPLSFKRFCAKVSMATSAIQYEN
                     DDLMMPLFDDDYSISCCVSAMRTGKEMQYFGARCNLPKLLLLALNGGKDEITGAQIAP
                     EFESYEDEYLDYDKVMVLFKNLQKWLARLYVKTMNVIHYMHDKYAYERLMMALHDTDV
                     KRNMAFGIAGLSVIADSLSAIKHTRVKAIRDERSLITDFEIEGKYPQFGNADDRVDSI
                     AVEIVKDFYSELKKCSTYRNATHTLSILTITSNVVYGKKTGSTPDGRKIGEPFAPGCN
                     PMHGRDKKGALAVLNSVSKIPYKYCKDGISLTLSLIPNSLGKDTDSRQTNLVSLLDGY
                     FVQGGHHININVLEREILKKAMDDPASFPQLTIRVSGYAVNFIRLTREQQLEVISRTF
                     HGVM"
BASE COUNT          860 a          549 c          618 g          658 t
ORIGIN      
        1 tgcaaacact gaaaagaagg ttccaagaga gtggataaac gaagaaggca atggcataaa
       61 acagggctat atagactatg cattgcccct tattcaaggt gagtcaaagc cacccattga
      121 ggatggtctt ccaaggtttg ctaaactgaa aaaggtattg gcaaaaaagt aatttgttta
      181 taacctaata aaacaagaga aatcatacga ctggcggaag tggagataac cacagggagt
      241 atgatttaat agatagccga ccgcctgggt aaaattaata ctcaggcggt ttttatatat
      301 atgaaacctg attttgcttc ataccgaagt caaagctttc aagctttagg ggggataaaa
      361 atgattgaaa gtgcatggag gaatttcaaa cccggagcat ggcaaaaaga aatagacgtg
      421 cgggatttca tcgtaaaaaa ttatacaccc tacgatggca gcgaagactt tcttgttcag
      481 tcaagcgaca gaacaagaaa gctatgggat aagtgcaaag agcttttaag agaagagtat
      541 ttaaagtcag gggttctgga tattgataca agcaccgttt ccggaatcac agcctataag
      601 cccggatata tcgataagga ttgcgaagta ataaaagggt tgcaaaccga cagcccttta
      661 aaaagagcag tcaatcctta cggcggcata agaatggcaa aacaggcctg tgaacggtat
      721 gactacagct tatcgcccca aatcaataaa atttttacag aatttcgtcg aacgcataat
      781 gacggtgtat acagctgtta cactcctgaa atgagaaggg caagaaaatg cggtgttata
      841 acgggactgc ccgatgctta cggccgcggc agaataatcg gagactacag gcgggtcgcc
      901 ctctatggcg tcaaccggct catcgaaggt aaaaaagacg atttaataca gttggaagac
      961 gtggatatgt cggaagaaac catacgactg cgtgaagaag tatttgaaca aattaatgca
     1021 ctgcaggaac tggttttgat ggcccaaagc tacggatgcg atatcacaaa acccgcagaa
     1081 gactcccttg aagccgttca gtggacatat tttgcttttt taggtgcgat gaaggaaacc
     1141 aacggtgcag ccaattccct cggaagagtc agtacattcc tggacatata tatagacagg
     1201 gatatcaagg aaggcaaaat caccgaatcc gatgcccagg aactgattga ccagtttgtg
     1261 gtaaagctcc ggctcataag acaccttagg actcccgatt acaacgagct ttttgccggt
     1321 gaccccgttt ggataaccga gtccatcgga ggaatgtgcg aagacggcag gcatatggtg
     1381 acaaaaacct ctttccgttt tctccaaacc ctttttaacc ttggtgcggc tccggaaccg
     1441 aatattacta tattatggtc cgaaaggctg cccctgtcct ttaaaaggtt ttgtgccaag
     1501 gtatccatgg ctacaagtgc catacaatat gaaaacgatg acctgatgat gccgctgttt
     1561 gacgatgact acagcatatc ctgctgtgtt tcagctatgc gtaccggaaa agaaatgcaa
     1621 tatttcggag caagatgtaa tcttccaaag cttttgcttt tagcattaaa cggcggaaaa
     1681 gatgaaataa ccggtgccca gatcgccccg gaatttgaat cctacgagga tgaatatctt
     1741 gactacgaca aggtaatggt tctgtttaag aaccttcaaa aatggcttgc aaggctttat
     1801 gtaaaaacaa tgaacgtcat acactacatg catgataaat atgcctatga aaggcttatg
     1861 atggcccttc acgacaccga tgtcaaaaga aacatggctt tcggcatagc aggcctttcc
     1921 gtgatcgccg attcgttaag tgccataaaa cacacacgag tcaaagcgat acgggatgag
     1981 aggagtctta taaccgactt cgaaattgag ggaaaatatc cgcaattcgg taatgcggac
     2041 gacagggttg acagtattgc tgttgaaatt gttaaagact tttacagcga attaaaaaaa
     2101 tgcagtacat ataggaatgc cacccacacc ctatctatat taaccataac ctcaaatgtg
     2161 gtgtacggca aaaaaacagg ttcaactccc gacggaagaa aaataggaga accctttgca
     2221 cccggatgca atcccatgca tggaagggat aaaaagggag cccttgctgt attgaactct
     2281 gtttcaaaga ttccttataa gtactgcaag gacggtattt ctcttactct gtccctgatt
     2341 cccaactccc tgggtaaaga cacggattca aggcaaacta atcttgtatc cttgctggac
     2401 ggttatttcg ttcaaggggg acatcatata aacattaacg tgcttgaaag ggaaatactg
     2461 aaaaaagcga tggatgaccc tgcaagtttc ccgcagctta ccataagggt ttcaggctat
     2521 gccgtcaact ttatacgtct aacccgtgaa cagcaactgg aggtaatttc aaggaccttt
     2581 cacggtgtaa tgtagggccg gagccgttta aaaggctcca gactgcaagg ctaaaattca
     2641 gggagtgttt tcttttgatt ttacttcatc ccttaagtgt tcgtc
//
LOCUS       DAOOJC010000340         2676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_29704_length_2676_cov_4.322014, whole genome shotgun sequence.
ACCESSION   DAOOJC010000340 DAOOJC010000000
VERSION     DAOOJC010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29704_length_2676_cov_4.322014"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLA01_08690"
     CDS             complement(<1..481)
                     /locus_tag="PLA01_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPD01407.1"
                     /translation="MKTTLLKELYRGTGDYLNKDIVVAGWVRTLRDSKVFGFIELNDG
                     SFFKNLQIVFEEQNIDNFKEVTKLPVGSSIIAYGKLVETPGAKQPFELKADKIEIEGI
                     SNSDYPLQKKRHSFEFLRTIAHLRPRTNAFSAVFRIRSLASYAIHKFFQERGFVYVHT
                     "
     gene            complement(571..1593)
                     /gene="asnA"
                     /locus_tag="PLA01_08695"
     CDS             complement(571..1593)
                     /gene="asnA"
                     /locus_tag="PLA01_08695"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HPD01408.1"
                     /translation="MIKKLDRLIIPMDYRPILSVRDTEIAIKKIKDFFEASLASELNL
                     TRVSAPLFVKPETGMNDNLNGVERPVSFEVKSIGGEKVEIVHSLAKWKRVALGKYGFK
                     YGEGLYTDMNAIRRDEDVDNLHSIYVDQWDWEAIINREDRNEETLKSIVRKIFNALKK
                     TEEHVFKAYPHIPKTLPEEIFFITTQELEDMYPKLSPKEREEAVCRDKKAVFLMKIGG
                     VLKSGIRHDGRAPDYDDWTLNGDILLWYPLLGTAFEISSMGIRVDEDSLMHQLKEAGC
                     QERTKLKYHRDLLEKKLPYTVGGGIGQSRLCMYFLKKAHIGEVQSSIWPDSMIDACES
                     ANIYLL"
     gene            2087..2626
                     /locus_tag="PLA01_08700"
     CDS             2087..2626
                     /locus_tag="PLA01_08700"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPD01409.1"
                     /translation="MTVGCSKSKYSVEHPKVQIEMEDGGKMVLELYPEYAPKTVDNFI
                     NLVENGFYDGLTFHRIIKGFMIQGGAPNSDSPPVEPIKGEFASNGFEQNTLKHTKGVI
                     SMARTRDKDSATSQFFIMHGDAPHLDGDYAAFGKLIEGEDVLDKIADTPVEENPYMSG
                     ELSLPKEEVIIKKATVLEK"
BASE COUNT          834 a          574 c          446 g          822 t
ORIGIN      
        1 gagtatgcac atatacaaat cccctctcct ggaaaaactt gtgtatggca taggacgcaa
       61 gcgaccttat cctgaacact gctgaaaaag catttgtcct tggccttaaa tgggcaattg
      121 tccttaaaaa ttcaaaagaa tgccttttct tttgaagagg ataatcggaa ttggatatac
      181 cttcaatctc aattttatcc gccttcagtt caaatggctg ctttgcaccc ggagtttcaa
      241 caagctttcc atatgcaatt attgaagagc ccacgggtag ctttgttact tccttaaaat
      301 tatcgatatt ctgttcttca aatacaattt gaaggttttt aaaaaaagaa ccgtcattga
      361 gttctataaa accaaatact tttgaatccc ttaaggttcg aacccaacct gcaacaacta
      421 tatccttgtt caaataatcc ccggttcctc tataaagttc ttttaacaat gtcgttttca
      481 tttcctaacc cctttatcaa ttaatattct ataaattatc atatattaat tatatatctt
      541 atccaataaa tcaataaact ataaataata ttatagcaaa taaatatttg cactttcgca
      601 ggcatcaatc atcgaatccg gccatatcga cgactgaacc tctccaatgt gtgctttttt
      661 gagaaaatac atacacagcc gggactggcc tatgccgccc cccacagtat atggaagttt
      721 tttctccaac aaatccctat ggtatttaag ctttgtcctc tcttgacaac ctgcttcttt
      781 aagctgatgc atcagggaat cttcgtccac ccttatgccc atggatgata tctcaaaagc
      841 ggttccaaga agaggatacc acaatagtat gtcaccgttc aatgtccaat cgtcataatc
      901 cggtgccctt ccgtcatgtc ttatgcccga cttgagtaca cccccgattt tcatgagaaa
      961 aactgccttt ttatcccggc aaacagcctc ttccctttcc ttaggagaaa gcttgggata
     1021 catatcctcc aattcctggg ttgtaataaa aaatatttct tccggcagag ttttggggat
     1081 atgggggtat gctttgaaaa catgttcctc cgtctttttt aaagcattaa atatttttct
     1141 cactatagat tttaaggttt cttcatttct gtcttccctg tttattattg cttcccagtc
     1201 ccactggtca acatatatgg aatgaaggtt atctacatcc tcatcccttc tgatagcatt
     1261 catgtctgta tacagccctt caccatactt gaagccgtac ttaccaagag ccaccctctt
     1321 ccattttgca agagaatgca ctatctcgac cttctccccg ccaatagact taacctcgaa
     1381 acttacaggc ctttcaaccc cattgagatt atcattcata cccgtttcgg gcttaacaaa
     1441 aagcggtgcc gaaaccctgg taaggttcag ttccgaagcc agtgatgcct caaaaaaatc
     1501 ctttattttc tttattgcaa tttctgtatc tctaaccgat aaaatcgggc ggtaatccat
     1561 tggaattatt aatctatcta acttctttat cactagcatt ttcctcctaa taaccgttta
     1621 aaataattaa tttgcattat accgttttct ttttagccca tacattttaa tttgtacacc
     1681 aatcggtgta caaataatta attcacatta taccatttat tagactcaaa ttccaatagt
     1741 tataacactt ctgttcgaag aaaatttttc gatgtaataa aattttttta tcttccgagt
     1801 ttcaaccagg ctcaagatag ctcaccgtct gaaaggcaaa ggatacttaa cccttgcaag
     1861 aagtagagga catctatcac cttgttataa ttgggcacaa aaaaatatgt gaagaaaaat
     1921 atatacccaa ctgaagaata aatgtagact gttatagaaa agtgttgtat tatttaatat
     1981 ggaaatttcg aaactcataa aattatagat caggagggca aaaatgtctc gtaaaattat
     2041 tacaaaatta atttcaatat ttctgcttgt aacaatgaca ggagtcatga ccgttggctg
     2101 cagcaagtca aaatacagtg tcgaacatcc aaaggtccag attgagatgg aagatggagg
     2161 taaaatggta ttggaactgt atcccgaata tgcaccgaaa accgtggata atttcatcaa
     2221 ccttgttgaa aacggcttct atgacggatt gactttccac agaatcataa aaggctttat
     2281 gattcaggga ggtgccccca acagcgacag tccccctgtt gaacctataa agggcgagtt
     2341 tgccagcaac ggttttgaac aaaacacgct aaagcacaca aagggagtca tatccatggc
     2401 cagaaccagg gataaagatt ctgctacatc ccagttcttt attatgcacg gagatgcacc
     2461 gcatcttgac ggggattatg cagcttttgg aaagctgata gagggcgagg atgtattgga
     2521 taaaattgca gatacgccgg tagaagaaaa tccatatatg tctggagaat tatccctgcc
     2581 aaaagaggaa gtcataatta aaaaagcaac tgttctggaa aagtaactgt tattagtgct
     2641 agcaaaagat tccggccttt catataaaaa gccgta
//
LOCUS       DAOOJC010000341         2676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_29707_length_2676_cov_3.156429, whole genome shotgun sequence.
ACCESSION   DAOOJC010000341 DAOOJC010000000
VERSION     DAOOJC010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29707_length_2676_cov_3.156429"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PLA01_08705"
     CDS             <1..911
                     /locus_tag="PLA01_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPD01410.1"
                     /translation="LEVAASGGHNCIMIGSPGCGKTMIARRLPTILPSLTFEEALEVT
                     KIHSIAGNLPENTSLITQRPFRAPHHSISNTGLIGGGKIPKPGEISLAHYGVLFLDEL
                     PEFSRDVLEVLRQPLEDGVVTISRVNATLTYPARTTLICAANPCKCGNYLDETKECTC
                     TSNEIKKYLGRLSGPLLDRIDIHIEVASVNYKDLESPEEGEKSRVIRERVNKARRIQQ
                     ERYKGLNIFSNAELSPSMIKKYCKLDDKCKEILKSAFDRLGLSARAHNRILKVARTIA
                     DMEESENIKANHLLEAIQYRSLDRKM"
     gene            932..1702
                     /locus_tag="PLA01_08710"
     CDS             932..1702
                     /locus_tag="PLA01_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPD01411.1"
                     /translation="MTKLISWNVNGIRACIEKGFLDYFKNANADIFCIQESKVQEGQI
                     ELDLQGYHQYCNYAIKKGYSGTAVFTRIKPVSVVNGIGIEEHDSEGRVITLEYKNCYL
                     VNVYTPNAKRELERLDYRMKWEDDFRNYLVGLNKKKPVVVCGDMNVAHKEIDLKNPSS
                     NRRNAGFTDEEREKFTMLLEAGFIDTFRFFYPDKKDAYTWWSYMFNARSRNAGWRIDY
                     FCVSQNLKDRLLSASIHDEVLGSDHCPVELVMDSEVYS"
     gene            1862..>2676
                     /gene="dprA"
                     /locus_tag="PLA01_08715"
     CDS             1862..>2676
                     /gene="dprA"
                     /locus_tag="PLA01_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPD01412.1"
                     /translation="MNNKIKYWIWLSSVPGVGAVKSRKLLEHFKDIESVWNAKGDELV
                     ILPFLSRTDVINLTDEKIKKNADIQLENILKHGIKAVTIEDDMYPVSLKNIYDPPIVL
                     YMKGNIEKDEKFLAIVGSRRASSYGIKMAQTIAAELSKYGITVVSGMARGIDSYAHKG
                     AIEAGGRTIAVLGCGLDIVYPSENKKLMENIISSGACVSEYLPGTSPIPGNFPARNRI
                     ISGISLGVIVIEAGERSGSLITADFALEQGREVFALPGNVNNYNSTGTNKLIK"
BASE COUNT          856 a          457 c          649 g          714 t
ORIGIN      
        1 ctcttgaggt ggcagccagc ggtggacata actgcattat gatcggctcg ccgggatgcg
       61 gtaaaacaat gattgccaga agactgccca caattcttcc gtctctgacc tttgaagaag
      121 cccttgaagt gacaaaaatc cacagtatag caggcaactt gcctgaaaat acttccctta
      181 ttacccagag gccgtttaga gcacctcacc attctatctc aaacaccgga cttatcggag
      241 gagggaaaat accaaagccc ggtgaaatta gtcttgccca ttatggagtg ttgtttttgg
      301 atgagcttcc ggagtttagc agggatgttc tcgaggtatt aagacagccg ttagaggacg
      361 gagtggtaac catttcgaga gttaatgcta ctttgacata tcctgcaagg actacattaa
      421 tatgtgccgc caacccttgt aaatgcggca actaccttga tgaaacaaaa gagtgcacat
      481 gcacgtcaaa cgagattaag aagtatttag gccgtttatc cggacctcta ttggacagga
      541 tagatataca tattgaggtt gcttccgtga actataagga tcttgaaagt ccggaagaag
      601 gagaaaaatc acgggttata agggaaaggg taaacaaagc gagaaggatt caacaggaga
      661 gatacaaagg gcttaatata ttttccaatg cagagctttc tccgtcaatg ataaaaaagt
      721 actgtaaatt ggatgacaaa tgcaaggaaa ttttgaaaag tgcttttgac aggcttggat
      781 tgagtgcccg ggcccataac agaattttaa aggtggcacg tactattgct gacatggagg
      841 aaagtgagaa tataaaggca aatcatctgt tggaggcaat acagtacaga agtctcgaca
      901 gaaaaatgtg attttagagg ggagattaaa aatgacaaag cttatttcat ggaatgtaaa
      961 cggcattagg gcttgtatag aaaagggttt tttggattat tttaaaaatg caaatgccga
     1021 tatattttgt atacaggaat caaaggttca ggaggggcag attgagcttg atcttcaagg
     1081 gtatcaccag tattgtaact atgcaataaa gaaggggtat tccggtactg cggtgtttac
     1141 aaggataaag ccggtctcgg ttgtaaacgg aatcggtatt gaagagcatg acagcgaggg
     1201 gcgggtgata acccttgaat ataaaaactg ctatcttgta aatgtgtata ctcccaatgc
     1261 caaaagagag ttggaaaggc tggattaccg gatgaagtgg gaggatgatt tcagaaacta
     1321 tttggttggg ctgaataaga aaaagcctgt tgttgtatgc ggtgacatga atgttgccca
     1381 taaagagatt gaccttaaaa acccgtcatc caacagaagg aatgcaggtt ttaccgacga
     1441 agaacgggag aagtttacca tgcttcttga agctggcttt attgatacct tcaggttttt
     1501 ttatcccgac aaaaaggatg cctatacatg gtggtcatac atgtttaatg cccgttcaag
     1561 aaatgccgga tggagaatcg attatttctg tgtgtcgcag aaccttaaag accgtctttt
     1621 gtcggcatcc atacatgatg aagtgttggg ctccgaccat tgccctgttg agcttgtaat
     1681 ggactctgaa gtttattctt aaatacagat gactgctttg ccaaagaaaa ggccgataat
     1741 ttgtcatggt tttcaaccga tatatcatat caagacttga ttcaatgaaa aatacatgat
     1801 aaattatttc atgacaataa aaaggttggt ttataccata agctgacgaa ggggtatact
     1861 tatgaataat aaaattaagt attggatttg gctgagttct gttccgggag ttggtgcagt
     1921 aaagtccaga aagcttctgg agcattttaa ggatatagaa agtgtctgga atgcaaaagg
     1981 ggatgagctg gttattctcc cttttttaag taggacggat gttataaact taaccgatga
     2041 gaaaataaag aaaaatgccg atatacagtt ggaaaatatc ttgaaacacg gcattaaagc
     2101 ggttacaata gaggacgata tgtatcctgt aagccttaaa aatatatatg atccacccat
     2161 tgtattgtat atgaaaggaa acatcgaaaa agatgaaaaa tttcttgcaa tagtgggttc
     2221 caggagagca tcgtcctatg gtataaagat ggcccaaacc attgccgcag aattatcaaa
     2281 atacggtatt actgtggtga gcggcatggc aagaggaatt gattcctatg cccataaggg
     2341 tgcaatagag gcaggaggaa ggactattgc agttttgggc tgtggacttg atattgtgta
     2401 cccttctgaa aacaaaaagc taatggaaaa cataatctca agcggtgcat gcgtatcgga
     2461 atacctgccg ggcacatctc ccataccggg taattttcct gcacgcaaca ggattataag
     2521 cggtatttcc cttggggtaa tcgtaattga ggcaggggaa cgcagcggtt cgttgattac
     2581 ggctgatttt gctcttgagc agggaagaga agtattcgcc cttcccggaa atgttaacaa
     2641 ctataacagt accggtacaa acaaacttat taagga
//
LOCUS       DAOOJC010000342         2672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_29766_length_2672_cov_4.186855, whole genome shotgun sequence.
ACCESSION   DAOOJC010000342 DAOOJC010000000
VERSION     DAOOJC010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29766_length_2672_cov_4.186855"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PLA01_08720"
     CDS             <1..848
                     /locus_tag="PLA01_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPD01413.1"
                     /translation="AVYDIKIAYVDEDDGNCTYTVDIKGKEVASWVADKNPTKDSIFI
                     YSINDVKLDYATEIKITGTRNKGEGARIAYLDITYSSGMSIKSVLKYTFSYLINNMPV
                     TSIFVLLLLIFTFLIVNILFIILFRPRKKSPEISHKSSKQPDNSSIVNDNSNGISDED
                     PVTSTTDTSYQTVDTSLIDKMKMYLDNNFSDTNFSVQEMADNFNLSINYLSSYFKEQT
                     SENIINYITCLRIEKAKHLLVTTDFPIKKIAESSGYYNVSSFIRRFRQITGVTPGKYR
                     EDNMG"
     gene            complement(987..2615)
                     /gene="hcp"
                     /locus_tag="PLA01_08725"
                     /gene_synonym="priS"
     CDS             complement(987..2615)
                     /gene="hcp"
                     /locus_tag="PLA01_08725"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPD01414.1"
                     /translation="MSMFCYQCQETAGGKGCTVRGVCGKNEEVAKLQDLLVYTLKGIS
                     NIVTKGKIDIEKLGDANYEVLSSLFMTITNANFDADSIEKQIRKMLAIRDGIKKSVTV
                     GDLHDAATFIVGSRESMLEKADSAGVLSTKDEDVRSLREMITYGVKGMAAYAEHAKNI
                     GKENTEIYAFIYEALAATIDDTLSADELVGLTLKTGEYGVKVMALLDEANTSRFGNPE
                     ITEVNIGVRKNPAILISGHDLTDLEQLLEQTKGTGVDVYTHSEMLPAHYYPAFKKYDN
                     FVGNYGNAWWKQTQEFESFQGPILFTTNCIVPPRSEEVRKRIFTTGSTGFPGCKHIEA
                     DENGKKDFSEIIELAKTMPAPEEIETGSIVGGFAHNQVMSLADKVVEAVKSGAVKKFF
                     VMAGCDGRMKSRDYYTEFAKNLPGDTVILTAGCAKYRYNKLKLGDIGGIPRVLDAGQC
                     NDSYSLAVIALKLKEVFGLDDINKLPIAFNIAWYEQKAVIVLLALLYLGVKNIHLGPT
                     LPTFLSPNVAKVLVEKFGIAGIGTVEDDIRLFMN"
BASE COUNT          786 a          609 c          454 g          823 t
ORIGIN      
        1 ctgcagtcta tgacataaaa attgcctatg ttgatgaaga tgacggcaac tgtacatata
       61 cggtcgatat caagggtaag gaagtcgcat cctgggtcgc cgataaaaat cctacaaaag
      121 acagtatttt tatttactct ataaatgatg tcaagctgga ttatgcaaca gagataaaaa
      181 taaccggtac aagaaacaaa ggtgaaggag cacgaattgc ctaccttgat atcacttata
      241 gcagcggcat gtcaataaaa tcggttttaa aatacacatt ttcttatctt atcaacaaca
      301 tgcccgttac ctctattttt gtcttattgc ttttaatttt cacattctta attgtcaata
      361 tccttttcat tatattattc cgacccagga aaaaaagtcc ggaaatttca cataagtcca
      421 gtaaacaacc ggacaacagt tctatcgtta atgacaacag taacggtata tccgacgagg
      481 acccggtcac aagtacaaca gatacttcat accaaacggt cgatacctct ttgatcgata
      541 aaatgaaaat gtatttggac aataattttt ccgatacaaa tttctccgta caggaaatgg
      601 ctgataattt taatttatct ataaactact tgagcagtta ttttaaagaa caaaccagcg
      661 aaaacattat taattatatt acttgtctca gaattgaaaa agccaaacat ttacttgtta
      721 caaccgactt tccgataaaa aaaattgccg aaagctccgg atattacaat gtatccagct
      781 tcatcaggcg ttttagacag ataacgggag tcaccccggg caaatacagg gaggataaca
      841 tgggttaatt ataatgctgt tttaattcga ataaaacaat aactcagttg acagtcttta
      901 tgctgttgac tcaatgaata tgaagacaga aaatgaagtc ccggcaacgt gatttattca
      961 tatcgccggg acttttatta ttaaaatcag ttcataaaca atctgatgtc atcttcaacg
     1021 gtaccgatac ccgcaatacc gaacttctca actaaaacct ttgccacatt cggtgacagg
     1081 aacgtcggca gcgttggtcc aagatggatg ttcttcacac ccaggtacaa aagtgccaat
     1141 agcactatca cggccttctg ctcataccat gctatattga atgcaatcgg cagcttgttg
     1201 atatcgtcca agccaaatac ctctttaagc ttcagtgcaa taactgccaa tgaatatgaa
     1261 tcattgcact gtccggcatc cagcactctc ggaataccgc cgatatctcc cagcttcaac
     1321 ttattgtaac ggtatttggc acagccggcc gtcagaatta cggtgtcccc cggaaggttt
     1381 ttggcaaact cggtgtaata atccctggat ttcatacgtc catcgcaccc ggccattaca
     1441 aaaaactttt tgacagctcc ggatttgacg gcttctacca ccttatctgc aagactcatc
     1501 acctgattat gggcaaatcc tcctacaatg ctgccggttt caatttcttc cggtgcaggc
     1561 atagttttag ccaattcaat aatttcagag aaatcttttt taccgttttc atcagcttca
     1621 atatgtttgc agccaggaaa acccgtagaa ccggttgtaa atattctttt tcttacctct
     1681 tcactccttg gcggtacaat gcagtttgtg gtaaacagaa tgggtccctg gaaggactca
     1741 aattcttgtg tctgcttcca ccatgcgttt ccgtagttgc ccacaaaatt atcgtacttt
     1801 ttaaaggccg gataataatg ggcaggcagc atttcactat gggtatatac gtccactccg
     1861 gttccctttg tctgttccaa aagctgctca agatccgtaa ggtcatgccc ggatataagt
     1921 atggccgggt tctttctcac gccgatatta acctcggtga tttccggatt tccaaatctc
     1981 gatgtgtttg cctcatccag aagtgccatg acttttacac catactcacc ggtttttaga
     2041 gtcagtccga caagttcatc cgctgaaagt gtgtcatcta tggtggctgc taaagcttca
     2101 tatataaaag cataaatttc cgtattttct ttcccgatat tttttgcatg ctcggcatag
     2161 gctgccatac ccttaacacc gtaggtaatc atttccctca aagaacgtac atcttcatct
     2221 ttggttgaca atacacctgc tgaatctgcc ttttcaagca tagattccct cgaacccaca
     2281 ataaaggtgg ctgcatcatg taaatcgccc acagttacgg attttttaat accgtccctt
     2341 atggcaagca tctttcgtat ctgcttttca atagaatcag catcaaaatt tgcattggta
     2401 attgtcatga acaaactgct caacacttca taatttgcat cgccaagctt ctcgatatcg
     2461 attttccctt tagtaacaat atttgaaata ccttttagag tatatacaag aaggtcctga
     2521 agctttgcaa cctcctcgtt tttaccgcat actccacgca ctgtacaacc ctttcctcct
     2581 gctgtttcct gacattgata acaaaacata ctcatttttt attcctccta ttaatttttt
     2641 ttagttttat gattcaattt ttttatctta ca
//
LOCUS       DAOOJC010000343         2649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_30091_length_2649_cov_3.813416, whole genome shotgun sequence.
ACCESSION   DAOOJC010000343 DAOOJC010000000
VERSION     DAOOJC010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30091_length_2649_cov_3.813416"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PLA01_08730"
     CDS             complement(<1..690)
                     /locus_tag="PLA01_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD01415.1"
                     /translation="MEIINLGNRIMNTYLIKTEYCCLLIDTGYAEQFSGFYNKLASRN
                     IGLNEIDYVFLTHAHDDHAGFLNDVLQHTNAKVIMHYKALEGLRRGQNSFDGGCSGKL
                     ALLFCKIMGLFSKGEHKFPQIKKCFEERCILIDSNNKNDIESKIHGKIIETPGHTSCS
                     ISLHMDDGSLFCGDAAMNGFPSIQRITIWINNLREFYKSWELMIDTNPRMIYPAHGAP
                     FPISDLAKFLSK"
     gene            complement(707..1627)
                     /locus_tag="PLA01_08735"
     CDS             complement(707..1627)
                     /locus_tag="PLA01_08735"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HPD01416.1"
                     /translation="MQDLFNESIQNIKRAHIIYFTGTGGTARVSSQFEESLTSHGIEV
                     QKAALDMQEAEYCTLSTNIQKDSLLILIFAVHAFDAPEPVYDWINAIPDGIGRPAVVI
                     SVSGGGEVWPNTACRAGCIKLLERKGYNVFYERMMVMPSNILVATKDQLAIRLLQVLP
                     TKAENCVSEILSGVRRRRRAPIMSRIMTAIFKSEKKWAKKFPEYLRIGETCTGCGWCL
                     ENCPRKNIELVNSRPSFGGQCVACLRCIYGCPYKSIYIHRIGFITIKEGYDLDELEQR
                     MSGVALEPIEKIKAGILFIGVRDYLLNKDV"
     gene            complement(1673..>2378)
                     /locus_tag="PLA01_08740"
     CDS             complement(1673..>2378)
                     /locus_tag="PLA01_08740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HPD01417.1"
                     /translation="VVKYTVIDCEGVEPQMHGPAVASILVGKDCGVAPGASLYYWAEP
                     SWKGDYKYRTTALNQIIEYNKNKPLKKKIRVVSVSKGFSTDEDNLSMWKEKIKEAEEN
                     GIIIVHCSDEGDERFSGIGCKLYEDRDNPKNYDLCYFANNKSFRKPINSKYVPIDNRT
                     TADYTGENDYAFWADGGLSWGAPYLAGVIALGFQVNPNLEEKEIYQYLRETGTPFNGG
                     FIVNPKAFIKKVNELE"
     assembly_gap    2379..2388
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2389..>2649)
                     /locus_tag="PLA01_08745"
     CDS             complement(<2389..>2649)
                     /locus_tag="PLA01_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase S8"
                     /protein_id="HPD01418.1"
                     /translation="MEGRSIKDLDLTGEYDTLLKVTFDTRTEWPSKDKLPKEFNPQEI
                     IEIGKDPGLGIRDLHNQGITGQGVNVAIIDQPLLLGHKEYKD"
BASE COUNT          728 a          588 c          411 g          912 t
ORIGIN      
        1 ttttgataaa aactttgcca gatcagaaat cggaaaaggg gctccgtgtg caggatatat
       61 cattctagga tttgtatcaa tcatcagctc ccatgatttg tagaattcgc gaagattatt
      121 gatccaaatc gtgattcttt gaatacttgg gaatccattc atagcagcat ccccgcaaaa
      181 cagagaacca tcatccatat gcagagagat tgaacaagat gtatgtccgg gtgtttcaat
      241 tattttgcca tgtattttac tctcaatatc atttttgtta ttactatcta tcagtatgca
      301 cctttcttca aaacatttct ttatctgagg gaacttgtgt tcccctttgc taaacaagcc
      361 cataattttg cagaacagca atgccaattt accggaacag cctccatcaa aggaattttg
      421 ccctctgcgt aaaccttcaa gtgctttgta gtgcattatt acctttgcgt ttgtgtgctg
      481 caaaacatca tttaaaaacc cggcatggtc atcgtgagca tgagtcagga aaacataatc
      541 tatttcattt aaaccaatat ttctggaggc tagtttatta taaaagccgg aaaactgttc
      601 cgcatatccg gtgtctataa gtaaacagca atattctgtt tttattagat atgtgttcat
      661 tattctattg cccaagttga taatttccaa aacatcacct cataaattac acatctttat
      721 tcaacaagta atcccgaacc cctataaata gaattccagc tttaatcttc tcaattggtt
      781 caagtgctac accactcata cgttgttcca gttcatccag gtcatatcct tctttgattg
      841 taataaaacc aatcctatgg atatatatag acttatacgg gcagccgtaa atacaacgaa
      901 ggcacgctac acactgcccg ccaaaagaag gacgggaatt gaccagttca atgttttttc
      961 ttgggcagtt ctctaagcac cacccacagc ctgtgcatgt ttcgcctatt cgaagatatt
     1021 ccgggaattt ttttgcccat ttcttctcac ttttaaaaat tgcggtcata attcttgaca
     1081 taattggtgc tcttctcctg cgtcgaactc cggagagaat ttccgataca cagttctctg
     1141 ccttcgtcgg cagtacctgc aatagcctta tggcaagctg atcttttgta gcaaccaaaa
     1201 tatttgacgg cataaccatc atccgctcat agaatacatt gtaccctttc cgctctaata
     1261 gttttataca gccggcccgg catgcagtgt ttggccaaac ctctccgccg cccgaaacag
     1321 aaataacgac tgcaggtcgc ccgatcccgt cgggaatggc gttaatccaa tcataaaccg
     1381 gctccggagc atcaaaagca tgaactgcaa aaatcagaat cagtaaactg tctttttgta
     1441 tgtttgttga taaagtgcaa tattcagctt cctgcatatc caaagcagct ttttggactt
     1501 ctataccatg acttgtcagt gattcttcga attgtgatga aacacgtgct gttccgccgg
     1561 ttcccgtaaa atatattata tgtgctcttt ttatgttttg aatcgattca ttaaataaat
     1621 cttgcattat aattcctctt cagtttttat tacagataaa tttgaatcat atctattcca
     1681 actcatttac cttcttaata aatgctttag gatttactat aaaacctcca ttaaatggtg
     1741 tgccggtttc tcttaaatac tgatatatct ctttttcttc aagattaggg tttacctgaa
     1801 atcccaatgc aatcactcct gccagatatg gagcccccca gcttaatcca ccatcagccc
     1861 agaatgcata gtcattttcc ccagtataat ccgctgtggt cctattatct attgggacat
     1921 atttactatt tattggtttc cgaaatgatt tgttgtttgc aaaataacat aaatcataat
     1981 tttttgggtt atctctatct tcgtataact tacaacctat tccggaaaat ctttcatctc
     2041 cttcatcaga gcaatgaact attataatac cattttcctc agcttcttta atcttctctt
     2101 tccacattga taaattatct tcatctgtgc taaatccttt tgaaacactt actactctta
     2161 ttttcttttt taaaggtttg tttttattat actctatgat ttgatttaaa gcagttgttc
     2221 tatatttata gtctcctttc caggacggtt ctgcccaata atataacgat gccccaggtg
     2281 caactccaca gtcctttccg actaatatac tggctactgc aggcccatgc atttgcggtt
     2341 caacaccctc gcaatctatg acagtatatt ttactactnn nnnnnnnntt atctttatat
     2401 tctttgtgtc cgagtaacaa tggctggtca atgattgcga catttactcc ttgtcctgta
     2461 ataccttgat tatgcagatc tcttatccct aagcccggat cctttcctat ttcaattatt
     2521 tcttgtggat taaactcctt tggtaattta tcttttgatg gccattcggt acgtgtgtca
     2581 aatgttactt ttaacaaagt atcgtattcg cctgttaaat caagatcttt tatggatctt
     2641 ccttccatt
//
LOCUS       DAOOJC010000344         2639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_30245_length_2639_cov_4.335526, whole genome shotgun sequence.
ACCESSION   DAOOJC010000344 DAOOJC010000000
VERSION     DAOOJC010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30245_length_2639_cov_4.335526"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..866)
                     /locus_tag="PLA01_08750"
     CDS             complement(189..866)
                     /locus_tag="PLA01_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPD01419.1"
                     /translation="MVTGIEYINNDNNSLRAKVFSRIQNDILNGVYQPGDSLTEKKLC
                     EELGVSRTPVREAIRQLELEELVQSTPNKGVIVTGVTPKDIEEIYIIRMMIEGLAARW
                     ATEHITPEEVQELKDALDLEEFYTMKKDAEHILKVDSKFHDIIFRASKSKPLMHMLRT
                     FHHYVQRARNASFESPDRAKKVLEEHKAIFDAIVEGDADKAERVTKEHVKNASVAYKK
                     LIKDNKV"
     gene            complement(904..>2639)
                     /locus_tag="PLA01_08755"
     CDS             complement(904..>2639)
                     /locus_tag="PLA01_08755"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HPD01420.1"
                     /translation="NTLQAGFENADDHKYIQTVTSRHGIYFSRPGNGICHQVQLERFG
                     VPGMTLLGSDSHTPTGGGIGMLAIGAGGLDVAVAMGGGPYYMMMPKVCRVVLKGTLKP
                     WVTAKDVILEVLRVMSVKGGVGKIIEYAGEGVKSLTVPERATITNMGAELGATTSIFP
                     SDEVTKEFLKAQGREADWIELKADADAQYDEELVIDLDKLEPLAAQPHSPDNVAKVKD
                     IGKIKVDQVAIGSCTNSSYMDMMKVAAILKGKTVHPNVSLVIAPGSRQVLTMLAKNGA
                     LADMVAAGARILESACGPCIGMGQAPATDAVSLRTFNRNFEGRSGTKSAKVYLVSPET
                     AAASAITGVLTDPSELGEAPKVNMPENFVADDSMVLAPAPEGADVEVVRGPNIKPFPL
                     NKALSDRVEGKALIKVEDNITTDHIMPSNAKLLPFRSNIPYLSEFCLTPCDPDFPKRA
                     KENGGGFIIGGSNYGQGSSREHAALAPLQLGIKGVLAKSFARIHMANLINSGIIPMTF
                     ENEADYDEIGMNDELLIENARDQIKAGSGLVVRNVTKGKDIKVNVTLSERQVEIILAG
                     GLINYTKQQSK"
BASE COUNT          656 a          704 c          458 g          821 t
ORIGIN      
        1 cccgaggagt atttacaggc tctggccggc gaaacttaag tttctggaat attttcttca
       61 cctcacccca ctcaacattg gattcataaa caatcaacaa tctttctaca atgtcaaaag
      121 agctgccttt aactacagca gctcttcttt aggtttaacc ccgatttatt acctgggtta
      181 accccaattt acaccttatt atctttaatc agctttttat acgcaacact cgcatttttc
      241 acatgctctt ttgtgaccct ttctgcctta tccgcatctc cctctactat tgcgtcaaaa
      301 atagccttat gctcctccaa aacctttttg gccctgtccg gtgactcaaa ggatgcattt
      361 ctggctcttt ggacataatg atgaaatgtc cttagcatat gcataagggg tttgctcttg
      421 ctggccctga atataatatc atgaaacttt gaatctacct ttaaaatatg ttctgcatcc
      481 ttcttcattg tgtagaattc ttcaaggtcc aatgcatctt taagttcctg aacctcttcc
      541 ggcgttatat gctccgttgc ccaccgtgca gccaggcctt caatcatcat ccttataata
      601 tatatttctt caatatcctt tggagttacc cctgtgacaa tcactccctt attgggtgtg
      661 gactgaacca gttcctccag ctcaagctgt cttatagctt ccctcacagg tgttctgcta
      721 acaccaagct cttcacaaag cttcttttcg gtaagactgt cccccggttg gtatactccg
      781 ttcaaaatat cattttgaat ccgggaaaac acctttgctc tcaaagaatt attgtcatta
      841 ttaatatatt ctatacctgt caccattgca ctcccctttc gcagagcaca ttattttaat
      901 aatctacttg ctttgctgct ttgtatagtt tatgagtcct ccagcaagga ttatctccac
      961 ctgtctttct gataatgtaa cattaacctt tatgtccttt cctttggtta cgtttctcac
     1021 tacaaggccg ctgccggctt tgatctgatc tcttgcattc tctataagaa gttcatcatt
     1081 catacctatt tcatcgtagt ctgcctcatt ctcaaaggtc atgggaataa tacccgagtt
     1141 tattaggttg gccatgtgaa ttcttgcaaa ggattttgca agaactccct ttattcctaa
     1201 ttgcaatggt gccagtgcag catgttcacg gcttgaaccc tgtccgtagt tggaacctcc
     1261 gataataaac cctcctccgt tttccttagc tcttttcggg aaatccgggt cacagggtgt
     1321 aaggcaaaac tccgaaaggt acggtatgtt tgacctgaaa ggcaaaagct tggcatttga
     1381 aggcatgata tgatcagttg ttatattatc ttcaaccttt atcaaggcct ttccttccac
     1441 tctgtcggac aacgccttgt tcagagggaa cggcttaata ttcggtcctc tcactacctc
     1501 tacatctgct ccttccggtg ccggagctag aaccatgctg tcatctgcaa caaaattttc
     1561 aggcatattc accttcggtg cctcacccaa ttcagaagga tcggttaata caccggttat
     1621 tgcactggct gcagccgttt ccggactgac cagatagacc ttggcagatt tggtgccgct
     1681 tcttccttca aaattcctgt tgaaggttct caaagaaact gcatcggtag caggtgcctg
     1741 acccattcct atacaaggtc cgcaagcact ctccagaatt cttgctcccg cagcgaccat
     1801 gtcagccaat gcaccatttt tagctagcat tgtcaatacc tgtcttgaac ccggagcaat
     1861 aacaagactg acattgggat gaacagtctt tccctttaat atggcagcta ccttcatcat
     1921 atccatatag gatgaattgg tacagctgcc gatagccacc tgatccacct ttatttttcc
     1981 aatatcctta accttggcaa cattatccgg actatggggt tgtgcagcca aaggctccag
     2041 tttatcaaga tcaataacca attcttcatc atactgtgca tcagcatcag ccttaagctc
     2101 aatccagtct gcctctcttc cctgagcctt caagaattcc tttgtaacct cgtcactggg
     2161 gaaaattgat gtagtggcac caagctcagc acccatattg gttatggttg ccctttcagg
     2221 aacagtaagg ctctttactc cttcacctgc atactcaata atcttgccca ctcctccttt
     2281 taccgacata actcttaaaa cctcaagaat tacatccttt gcagtaaccc atggcttcaa
     2341 agtacccttt aaaaccactc tgcacacctt gggcatcatc atatagtacg gacctccgcc
     2401 cattgccaca gcaacatcaa gtccaccggc cccaatagcc agcataccta ttccgcctcc
     2461 ggtaggagta tggctgtccg accctaaaag agtcattccc gggacgccga atctttccag
     2521 ttgaacctgg tgacagatac cgttacccgg tcttgaaaaa taaatcccat gccttgaagt
     2581 aacagtctga atatatttat ggtcatctgc gttttcgaaa cccgcctgga gtgtattgt
//
LOCUS       DAOOJC010000345         2637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_30282_length_2637_cov_3.409760, whole genome shotgun sequence.
ACCESSION   DAOOJC010000345 DAOOJC010000000
VERSION     DAOOJC010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30282_length_2637_cov_3.409760"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1966
                     /locus_tag="PLA01_08760"
     CDS             <1..1966
                     /locus_tag="PLA01_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01421.1"
                     /translation="KFTFYAGMDPNDLMARFESLPCSAAEGDDVLVCVTVNSTFETDL
                     ANVPYKWEITDKNGNPVPATFSGSANAAEGKIEKIEAIKGESVLYAVFKMPDSDVNIK
                     FEVNKDGQNPQEKYLDNNVIDSGELIKVAKRIETSVEQFDLDYNVLSRKVAFPLADNN
                     DIVAELILPRSDASWNGSNATGALNVTNEAEKLFNNFNVKNNPPVNEPEEIIVRRPVI
                     NMTLNRSSFGDNPEGKKCLNWPTPWEPLSLNGNITFEGNVVRKYTYKINNRNNLPVDT
                     GSGEAKASFNSGVKTVNVRAFIYNGSPSIAPKTFQNTIENNNANSLEKKLLWASEPYK
                     FDVIRKMCHLNENGSVERWVDVPGQYQRIFTQQNSAKINWSIPASMAANYKRSRDAAR
                     KMDTRNSELDRAVFASDKAFQDLAYPIKSGYYFNPTGTYQFTVETVTYKPTTADTEEH
                     KNLVQALINSFRYESNLVYVNNKNQAVDIQNQPATKKGTVYTARAAAITAADPVGLNG
                     VNWLQIIDRKADPSRYTKTVEEIRHSTDEDISKTHEFWRNILEGYEESGTLSSYNNYK
                     YREYVKPGQTMYRITEKTTVTIIVNPQNIKAYTHIQMADGKYYVRAYFADSALANISS
                     ALPTLKSVQIDNIEISVVGSRYSDMNFDER"
     gene            2051..>2637
                     /locus_tag="PLA01_08765"
     CDS             2051..>2637
                     /locus_tag="PLA01_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HPD01422.1"
                     /translation="MKKLALILVLVCTFVTLFSIVPSAVELPLRVVINGTKLEFPDAQ
                     PFIDANGRTQTPARFIGEALGANVTWDGVQKKAVFESDGNTLVLYIGKREYDVNDTKK
                     QMDTEALLIDGRTFVPARYVAEALGATVEWDASIRTVYINTKDNPTSTPTPTQGETVK
                     YYDGVAFNDVTDVDAYGRITVEKSKEFLLNFTKHL"
BASE COUNT          920 a          461 c          568 g          688 t
ORIGIN      
        1 aaaattcaca ttttatgcag gcatggatcc caatgacctg atggcaagat ttgagagtct
       61 tccttgcagt gcagcagaag gagatgacgt tttagtatgc gtaaccgtta actcaacttt
      121 tgagacagac ttggccaatg ttccgtacaa atgggaaatt acagataaaa acggaaatcc
      181 cgtaccggct actttttccg gaagtgcaaa tgcagcagaa ggaaaaattg aaaaaataga
      241 ggcaataaaa ggagaaagtg ttctctatgc tgtctttaaa atgcctgaca gcgatgtcaa
      301 tataaagttt gaggtaaaca aagacggaca aaatccacaa gaaaagtatc tggataataa
      361 cgttatcgat tccggagaat taataaaggt tgcaaaaagg attgaaactt cagtggagca
      421 atttgatttg gattacaatg ttctatcgag aaaggtggca ttcccccttg ccgataataa
      481 tgacattgta gcggaattga tattgccgag atctgatgct tcatggaatg gtagtaatgc
      541 aaccggagca ctgaatgtaa caaatgaagc agaaaaatta ttcaataact ttaatgtaaa
      601 aaacaatcct cctgtaaatg agccggagga gataattgta cgaagaccgg taataaatat
      661 gacacttaac agaagctctt ttggagataa tccggaaggt aagaaatgtt tgaattggcc
      721 gactccatgg gagcccttat ccttaaatgg aaatattact tttgagggta atgttgttag
      781 aaagtataca tataaaataa acaatagaaa taatcttccc gtggatactg gttcaggtga
      841 agctaaagca tctttcaaca gcggagtaaa aacagtaaat gtaagggcat ttatatacaa
      901 cggcagtccc agcattgcac caaagacctt ccaaaataca attgaaaata acaatgccaa
      961 ttctttggaa aagaagcttc tttgggcaag cgaaccctat aaatttgatg taattcgcaa
     1021 aatgtgtcat ctgaatgaga acggttcagt agaaagatgg gtggatgtac cgggacaata
     1081 ccaaagaatc tttacacagc agaattcggc aaaaatcaac tggtcgatac cggcaagcat
     1141 ggcagccaat tataagcgga gcagagatgc tgcaagaaaa atggatacaa gaaattcaga
     1201 actcgataga gctgtttttg catcggataa ggcgtttcaa gacttggctt atcccattaa
     1261 gtcgggttac tactttaatc caacgggcac ttatcaattt acggtagaaa cggtaactta
     1321 taaaccaacc actgctgaca cagaagagca caagaacctc gtacaggcgc ttattaactc
     1381 ctttagatat gagtcgaatc ttgtgtacgt aaacaataaa aatcaggcag tggatataca
     1441 aaaccagccc gcaactaaaa agggtactgt ttataccgca agagcagcag ctatcaccgc
     1501 agccgatccg gttggtttga atggtgtaaa ttggcttcaa ataatagaca ggaaagcaga
     1561 tccttcccgt tacacaaaaa cggttgagga aatcagacac tcaaccgatg aagacatttc
     1621 gaaaacccat gagttttgga ggaatatttt ggagggttat gaagaatcgg gaacgctttc
     1681 aagctataat aactataaat accgggaata cgttaaaccc ggtcagacca tgtatagaat
     1741 aacagaaaag acaacggtga caattatagt caatccgcaa aacataaagg cgtacactca
     1801 tattcagatg gcagacggca aatattatgt ccgggcttat tttgccgatt ccgcacttgc
     1861 taatatcagc agtgcacttc cgacattaaa gagtgttcaa attgataata tagaaatatc
     1921 agttgttggt tccagatata gtgatatgaa ttttgatgaa cggtaaccat ctgcgattat
     1981 aaatgaagtt cccctatata ttgtatctag gggaacttta tttataaatc aatctgagga
     2041 ggattttgag atgaaaaagc ttgctttgat actagtactg gtttgtactt ttgtcacttt
     2101 attttctata gtaccttctg ccgtggaatt accgcttcgt gtagtgataa acggcacaaa
     2161 attggaattc cctgatgccc aaccttttat agatgctaac ggaagaaccc aaacacctgc
     2221 aagatttatt ggtgaagcat tgggtgcaaa cgttacatgg gatggtgtac agaaaaaagc
     2281 tgtatttgaa tcagacggaa atacattggt tttgtacatc ggaaagagag aatatgatgt
     2341 taacgacacg aagaaacaga tggatactga agctttgctt attgacggca ggacttttgt
     2401 tccggctaga tatgtagcgg aagcattagg tgcaacagta gagtgggacg catctatcag
     2461 aactgtatat ataaatacaa aggataatcc cacgtcaacc ccgacaccga ctcaaggcga
     2521 aacagtaaaa tattatgacg gtgttgcctt taatgatgta acagatgttg atgcttacgg
     2581 aaggataaca gtggagaaat ccaaggagtt tttgcttaac tttacaaagc atcttaa
//
LOCUS       DAOOJC010000346         2632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_30351_length_2632_cov_4.592938, whole genome shotgun sequence.
ACCESSION   DAOOJC010000346 DAOOJC010000000
VERSION     DAOOJC010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30351_length_2632_cov_4.592938"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PLA01_08770"
     CDS             complement(<1..539)
                     /locus_tag="PLA01_08770"
                     /inference="COORDINATES: protein motif:HMM:NF025057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HPD01423.1"
                     /translation="MKSKRNYEEEEVSEGSPEWLTTYSDMVTLLLTFFVMLFSMANID
                     KQKFEQVAHSLRAAFMNNNTEGMFDYKDGTEVYSINESVNSGDILEDGDEAFNMGQSS
                     EDGTDLKAKGGEAEKLEEFIERVEKLAEEMELDDYVKVIDEKTHLTLRIDSVILFDLG
                     KADIKASGKDALRKIGNLL"
     gene            complement(555..1388)
                     /locus_tag="PLA01_08775"
     CDS             complement(555..1388)
                     /locus_tag="PLA01_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HPD01424.1"
                     /translation="MDLATLIGIIAGVVFIVASIMTGGSIWSFWDISSVYITVGGGIC
                     ATLISYKIGEFAKIMKVAVKVLVGKESRPQDTIKMLIELSMKARREGLLSLEAEQENI
                     HEDFLKQSLQLVVDGVEPEVIVDSMDLELENMKARHDKGAGIFKTLAAQFPAWGMIGT
                     LIGLIQLLDQLDDPSAIGPAMAVALITTFYGSVLANFICNPIATKLSLKSKEEIQEKK
                     MIIEGVLSIQAGENPRILEHKLKTFLSPKQKQEYEMQSGKSADDAGRVENSENVRNAA
                     V"
     gene            1849..2583
                     /locus_tag="PLA01_08780"
     CDS             1849..2583
                     /locus_tag="PLA01_08780"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HPD01425.1"
                     /translation="MKKVKSLILYFLIYSFLLNLMFLSTTFHSIASISTNVKVYIDNE
                     LMEFGLESNDPGPYIKEGRTLIPFRRIFEALDMDVSWDDKERMVTAVGNDIEMKLYIG
                     KTIAYVNSTEKTLDVPAEITDGRTFVPLRFVSENCGAEVKWEDSSKSVFITLPKETAL
                     PEEDVPGAPGTIDKPVIEEKKLGEKVIYNGMVFSFDSVELLDTPDVREKKLRIRGTTN
                     IDDSTLWIEVYNEYHNNVAIRAFAQP"
BASE COUNT          786 a          558 c          441 g          847 t
ORIGIN      
        1 tttaaaagat ttccgatttt tcgcagtgca tcttttccgg aagcctttat atcggcttta
       61 ccgagatcga acagtataac agagtcaatc ctcaacgtca ggtgtgtctt ttcgtcaatt
      121 acctttacat agtcatccaa ttccatttct tctgccaact tttcaactcg ttcaataaac
      181 tcttccaact tctcagcctc tccacctttg gccttcaaat cagtcccatc ttcgctgctt
      241 tggcccatgt taaatgcctc gtcaccatct tccagtatat ctcccgagtt gacactctca
      301 tttatactat acacctcagt gccatcttta taatcaaaca ttccctcagt attgttgttc
      361 ataaatgcgg ctcttagcga gtgggcgacc tgctcaaatt tttgtttgtc aatattagcc
      421 atcgaaaaaa gcattacaaa aaatgttaat agcaacgtaa ccatatcact gtatgtagtc
      481 aaccactcag gagaaccctc ggatacttct tcctcttcat aattcctttt tgatttcatg
      541 aaaaatacac ctcactatac agcagcattt ctaacatttt cactgttctc aactcttccg
      601 gcatcatctg cacttttacc ggattgcatt tcgtattcct gcttttgctt cggagaaagg
      661 aaggtcttaa gcttatgttc caatattctc gggttctctc ctgcctgtat ggacagaaca
      721 ccttcaataa tcatcttttt ctcctgtatt tcctctttgc tctttaaaga caatttggtg
      781 gcaatagggt tgcatatgaa attggccaga acacttccat aaaatgttgt tatcaatgcc
      841 acggccattg ccgggcctat tgctgatggg tcatccaatt ggtcaagaag ctgtatcaag
      901 cctataaggg ttcctatcat accccatgcc gggaactgtg ccgccagcgt cttaaatatt
      961 ccggctccct tgtcatgcct tgctttcata ttttccagct caagatccat agaatcaaca
     1021 attacttccg gctcaacgcc gtcaacaaca agctgaaggg actgtttcaa aaagtcttca
     1081 tgtatgtttt cctgttctgc ctcaagagaa agtaatcctt ccctccttgc tttcatagaa
     1141 agctcaataa gcatcttaat tgtgtcctgg ggccttgatt cctttcccac caaaaccttc
     1201 acggcaacct tcataatttt ggcaaactcg ccgattttgt aggaaattaa tgttgcacag
     1261 attcctcctc ctacagtaat atatactgag gaaatgtccc agaatgacca aatactgcct
     1321 ccggtcatta tagaggccac tataaataca acaccggcaa taatacctat aagagtagct
     1381 agatccataa atctcgcctt ctatcctttt taatattcaa cctgcaataa ttatatcgga
     1441 acaatcatgt gaaaaattta ggaacttttt tagcgtattt tactattgtt ataacacttc
     1501 aattaaaatg aagttgcccg gtggtggaaa tatttccttg attaatactc tgtattgtca
     1561 taaatactct gtattgtcat agaacttgta attgtacata ttacaacact catagttaaa
     1621 ataaatatta aaattgttct ttctatggta aaatatactt tatttattga gcaatttatt
     1681 atataatatc atcaagataa tttttccttt tcaagaaaca taaacattaa ttaagcaata
     1741 ttatatatta agaaacttta tgatgttctt gttttcaatg ttttttacag ccgtaagtaa
     1801 tataataatg aaaaacaaaa acaaatttaa tcaacgggag gactagatat gaaaaaggtg
     1861 aagagtctta ttttgtattt tctcatctat tcatttttgc ttaatttaat gtttctatca
     1921 accacctttc atagcattgc atcaattagc accaatgtaa aagtgtatat tgacaatgag
     1981 ctgatggaat tcggtcttga gtcaaacgat ccgggtcctt atattaaaga aggaagaaca
     2041 cttattcctt tcaggaggat atttgaggct ctggatatgg atgtctcatg ggacgacaag
     2101 gaacgcatgg ttactgcggt aggcaatgat atagagatga aactctacat cggtaaaaca
     2161 attgcctacg taaatagcac ggaaaagact ctcgatgttc cggctgaaat aaccgatggc
     2221 aggacttttg tccctttaag gttcgtatca gaaaactgtg gtgccgaagt caaatgggaa
     2281 gacagcagca aatcggtttt tattacattg ccaaaagaaa ctgcattacc ggaagaggat
     2341 gtacccggag cacccggaac aattgacaag ccggtgatag aagaaaagaa acttggggaa
     2401 aaggttatat ataatggaat ggtcttttcc tttgattcag tagaactttt ggatacgccg
     2461 gacgttcgtg agaaaaaatt acgaataagg ggtacaacta atatcgatga ttccactctt
     2521 tggattgaag tttataacga gtaccataat aacgtagcaa tccgggcttt tgcacaacct
     2581 tgaatccgat acttatggct tcctcgcaac ctcgtatgtt tcaagctcat tt
//
LOCUS       DAOOJC010000347         2631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_30373_length_2631_cov_4.297360, whole genome shotgun sequence.
ACCESSION   DAOOJC010000347 DAOOJC010000000
VERSION     DAOOJC010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30373_length_2631_cov_4.297360"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..>2631
                     /gene="uvrA"
                     /locus_tag="PLA01_08785"
     CDS             193..>2631
                     /gene="uvrA"
                     /locus_tag="PLA01_08785"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPD01426.1"
                     /translation="MKRDSIIVKGAREHNLKNIDIEIPRDKFVVITGISGSGKSSLAF
                     DTIYAEGQRRYIESLSAYARQFLGQMEKPDVDYIDGLSPAISIDQKTTSRNPRSTVGT
                     VTEIYDYLRLLYARIGIPHCYSCGKEISQQTVDQMVDKIMGLEEGTRIHLLAPVVRGR
                     KGEYHKLIEDIKKGGYIRVRVDGEIMDVNDPITLDKNKKHNIEIVVDRLVVRPDIQKR
                     LADSIETVLHLSSGVLIVDVIGKEELLFSQNFACSECGISIEELTPRMFSFNNPYGAC
                     PACSGLGSMLKISPDLVIPDKSLSLAEGAISVGGWNISNEDGYARMYINALARHYNFS
                     VDTPLQDLPPEIVNMILYGNNGEKIKIEYEREYGKGSFMASFEGVINSMERRYKETQS
                     EGMKQYYEGYMSNNPCPECGGARLKKESLAVTVGDRNIYEISTIPVGELKQFFDNLKL
                     TERQKLIAHQILKEINARLGFLVDVGLDYLTLSRAAGTLSGGEAQRIRLATQIGSGLM
                     GVVYILDEPSIGLHQRDNDRLLTTLKKMRDLGNTLLVVEHDEDTMYASDYIIDLGPGA
                     GSHGGEIIAKGTVEEIKQNPNSITGQYLSGVKRIEVPEKRRKPNGKWLEVIGARENNL
                     KNINVKIPLGVFTCVTGVSGSGKSSLVNEILYKKLANDLNRAKQVPGEHDSIIGLENL
                     DKVINIDQSPIGRTPRSNPATYTGVFDIIRELFASTTEAKTRGYKSGRFSFNVKGGRC
                     EACQGDGIIKIEMHFLPDVFVPCEICKGKRYNRETLEVRYKGKNIAEVLDMTVENALE
                     FFKNVPRIKNKIQTL"
BASE COUNT          840 a          437 c          660 g          694 t
ORIGIN      
        1 tttttcttcc atattcttct tcacataaaa taatatgcat ccatttaata aaataacaaa
       61 gaatctgaag atgtataata taaaaaacac tcaaaaatca caaaaaaaaa atctcgaaca
      121 aatgtttgaa ttttgcagta tatctggtat aataatagct gtttatatta tagaggtgag
      181 caggtgtttg ttatgaaaag agacagtatt attgtcaaag gtgcaagaga gcataattta
      241 aagaatatag atattgagat tcccagggac aaatttgtgg taattaccgg tatcagcggc
      301 tcaggtaagt cctctctggc cttcgataca atttatgcag agggacaaag gcgttatatt
      361 gaatcgttgt ctgcttacgc aagacagttt ttaggacaga tggaaaagcc cgatgtggat
      421 tacattgacg gactgtcacc ggcgatatcc atagaccaga aaaccaccag ccgtaatccc
      481 cgttccactg tcggtacggt gacggaaatt tacgactacc tgagattatt atatgcaaga
      541 ataggtattc cccattgcta ctcctgtgga aaggaaattt cccagcagac agtggaccag
      601 atggtggata aaatcatggg gcttgaggaa ggtacacgga ttcatcttct tgctcccgtg
      661 gtcagaggaa gaaagggtga gtaccataag cttattgagg atataaaaaa aggcgggtat
      721 ataagggtca gggttgacgg tgaaataatg gacgtaaacg accccataac tcttgataag
      781 aataaaaagc acaacataga aattgtagtg gacaggcttg tggtcagacc ggatattcaa
      841 aagagacttg cggattctat tgaaacggtg cttcatttaa gcagcggtgt gctgattgtt
      901 gatgttattg gcaaggaaga gcttctattc agtcagaatt ttgcctgcag cgaatgcggt
      961 atcagtattg aagagcttac tccaagaatg ttttcattta ataaccccta tggggcttgc
     1021 ccggcttgca gtggtttagg ctcgatgtta aaaatttctc ccgaccttgt gataccggat
     1081 aagagtttgt ctttggccga aggtgccatc agtgtgggag gatggaatat atccaacgag
     1141 gacgggtatg cccggatgta tattaacgct ttggcaaggc attacaattt cagtgtagat
     1201 acaccgcttc aagatcttcc tccggaaatt gtgaacatga tattgtacgg aaataacggt
     1261 gaaaaaatca aaatagaata tgaacgggag tatggaaagg gttcgttcat ggctagcttt
     1321 gaaggtgtta taaacagtat ggagagacga tataaagaga cacagtctga gggcatgaag
     1381 cagtattatg agggatacat gagcaataat ccatgccctg aatgcggagg agccagactt
     1441 aaaaaagaaa gtcttgcggt tactgtcggg gataggaaca tatacgagat atccactatt
     1501 cccgttggag aattaaagca gttttttgac aacttaaagc ttacagaaag gcagaagctt
     1561 attgcccatc agatattgaa agaaataaat gcaagattgg gttttttagt ggatgtgggc
     1621 ctggactatc ttactctgtc aagggctgcg gggacgttat ccgggggaga agcccagagg
     1681 ataaggcttg caactcaaat cgggtccggt cttatgggag tggtgtatat actggacgag
     1741 cccagtattg gccttcatca gagggacaat gacaggcttc ttacaactct caagaaaatg
     1801 agggatttgg gaaatactct attggtagtc gagcatgatg aggacaccat gtatgcctcc
     1861 gattacatta tagatttagg tcccggtgca gggagccacg gtggggaaat aattgcaaaa
     1921 ggaactgttg aggaaattaa gcagaatcct aattctataa cgggacagta tcttagtgga
     1981 gttaaacgga ttgaggttcc cgaaaagaga cgtaaaccca acggaaagtg gctggaggtt
     2041 attggggcca gggagaataa cctgaaaaac ataaacgtaa aaatacctct aggggtgttt
     2101 acatgcgtaa ccggtgtttc gggttcggga aaaagttctc tggtaaatga gattttgtac
     2161 aaaaaacttg ccaatgatct taacagggcc aaacaagtgc cgggtgagca tgatagtatt
     2221 attggtctcg agaaccttga caaggtgatt aatattgacc aatcgcctat tgggcgtaca
     2281 ccgaggtcca atccggcaac atacacaggt gtttttgaca ttatacgaga gttgtttgct
     2341 tccaccactg aggcaaaaac gaggggttat aaatccggaa ggtttagctt caatgttaag
     2401 gggggacgtt gtgaggcttg ccagggtgac ggaattataa aaattgagat gcatttctta
     2461 ccggatgttt ttgtaccctg tgagatttgt aagggaaaaa gatataacag ggaaaccctt
     2521 gaagtaagat ataagggtaa gaacattgca gaggttcttg atatgacggt ggaaaatgcc
     2581 ctggagtttt ttaaaaacgt accgagaata aagaataaaa ttcaaacatt g
//
LOCUS       DAOOJC010000348         2619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_30552_length_2619_cov_4.691498, whole genome shotgun sequence.
ACCESSION   DAOOJC010000348 DAOOJC010000000
VERSION     DAOOJC010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30552_length_2619_cov_4.691498"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2619)
                     /locus_tag="PLA01_08790"
     CDS             complement(<1..>2619)
                     /locus_tag="PLA01_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPD01427.1"
                     /translation="WTSYSLTPGEVLEIKETFTMTAPTDDTCEFTFHLGGDKAGTVPY
                     RVFLDDVSLYDPEFTKPVIEELKQPDVRVNQVGYLPEGKKVATVVCDSKQPVKWNLLN
                     AAGVVVDEGYTEPIGLDEDSQDNVHWLDFSDFTTEGIGYRFELPTVETVTNYSHPFDI
                     RPDIYSQMKYESLAFFYHKRSGIEIEMPYAGREDLCRPAGHVDKGENTGDGSVPTWPQ
                     NDEYAGLPQKSYSKNVIGGWYDAGDHGKYVVNGGIAVWTMMNQYERAKINGLEDWGPY
                     KDGGMNIPEKNNDYPDILDEARWEIEFFKKMQVTSSEDSSIAGMVHHKIHDFRWTALG
                     MLPHEDSQPRYLRAVSTAATLNTAATLAQAARLWEEYDSSYAAECLEKAEIAWQAAKK
                     HPDILAEYTPGSGGPGGGPYNDDYVGDEFYWAACELFITTGKSEYKDYLMSSPHYLEM
                     PAKMEGTNEDGGLWGCFTWGTTAGLGTVSLALVENGLPESEIQKARNNIAEAADAWLD
                     NIEDQGYRLPIKQAEDERSGYPWGSNSFILNMMIVMGYAQDFTGDSKYLDGILDSMSY
                     LLGRNAMDQSYVTGYGERPLTNPHDRFWTPQTSAKWPSPPAGIVSGGPNSRFEDPTIN
                     AAVMKDTPPQKCFIDHTDSWSTNEITVNWNAPFAWVTAYLDEQYADSDSARVSIDSPV
                     AGTRFEAGKDVDISATVKADTTVSKVEFYNGDELISSDSSAPYTAKISKAAVGAYDIK
                     AVAVLSDGRRIESPITPILVKVIVKPTVTLTSPKSNVVIHGNEFLKITATGKDTDGKI
                     SKIEFLVDDKVIGSDKEAPFEFEWKAQEGNHKISVIAYDDDNAASSPASVNIFVKQAK
                     DVKVQYMCDSTQASNQ"
BASE COUNT          677 a          639 c          484 g          819 t
ORIGIN      
        1 tcctgattag atgcctgagt actatcacac atatattgta cctttacgtc tttagcctgc
       61 tttacaaaga tgttaacaga agccggtgat gaagcagcgt tgtcatcatc atatgcaata
      121 acacttattt tatgattgcc ctcttgtgcc ttccattcaa actcaaaggg tgcttccttg
      181 tccgatccta ttaccttgtc gtcaacaagg aattcaattt ttgaaatttt accgtcagta
      241 tccttacctg tagccgtaat cttcaggaac tcgtttccat gaattactac atttgactta
      301 ggtgaggtca atgtgacagt aggtttgaca attactttaa ccagaatagg agttatcggt
      361 gactcaattc ttcttccgtc ggacagaact gcaactgctt taatgtcgta tgcaccgaca
      421 gcagctttgg atattttggc tgtatatgga gcacttgagt cacttgaaat aagttcgtct
      481 ccattgtaaa actctacctt actgacagta gtatcagctt ttacggttgc acttatgtca
      541 acatccttgc cggcctcaaa ccttgttcct gcaactggtg aatcaatact tacccttgca
      601 ctatcactat cggcgtactg ctcgtcaaga taagctgtaa cccatgcaaa aggagcattc
      661 cagttaactg ttatttcgtt tgtcgaccat gaatcagtat ggtctataaa gcatttctgt
      721 ggaggcgtat ccttcattac agccgcattt attgtcgggt cctcaaaacg agagtttggt
      781 cctccggaaa ctatacctgc aggtggtgac ggccattttg cactggtctg tggagtccag
      841 aatctgtcat gaggatttgt aagcggacgc tcaccatatc ctgttacata tgactgatcc
      901 attgcgtttc ttcccaacag gtagctcata ctgtccaata ttccatcaag gtatttactg
      961 tctcctgtaa aatcttgagc atatcccata acaatcatca tgttaagaat aaatgagttt
     1021 gaaccccatg gataaccgct gcgttcatcc tctgcctgtt tgattggaag tctgtatccc
     1081 tgatcttcaa tattatcaag ccacgcatca gctgcttcag ctatgttatt tcttgccttc
     1141 tgaatttctg attccggtaa accgttttct accaatgcca aggaaacggt ccctaatcct
     1201 gctgtggttc cccaagtgaa gcaaccccat aatccgccgt cttcgttggt gccttccatc
     1261 tttgcaggca tttcgagata gtgaggtgaa ctcatcaagt aatccttata ttcggattta
     1321 ccagtagtta tgaaaagctc acatgctgcc cagtagaact cgtctccaac atagtcgtca
     1381 ttgtatggtc caccaccggg gccaccgcta cccggagtat actctgcaag tatatcagga
     1441 tgcttctttg ctgcttgcca agctatttca gccttttcca agcactcagc tgcataagaa
     1501 gaatcatatt cttcccacaa acgtgcagcc tgtgcgagag ttgctgcagt gtttaatgtt
     1561 gctgctgtac taacagctct taaataacga ggttgtgaat cctcgtgagg caacataccc
     1621 aatgctgtcc atctgaagtc atgtatcttg tgatgaacca ttcctgctat ggaagagtct
     1681 tcacttgatg ttacctgcat cttcttaaag aactctattt cccatcttgc ttcatcaagg
     1741 atatcgggat aatcgttgtt cttctcaggt atgttcattc cgccgtcttt ataaggtccc
     1801 cagtcttcaa gaccgttaat ttttgctctt tcatactggt tcatcattgt ccaaacggca
     1861 ataccgccgt ttacaacata tttaccatgg tcaccggcat cataccatcc gccgattaca
     1921 ttctttgaat agctcttctg aggtaatcca gcatactcgt cattttgagg ccatgtcgga
     1981 acacttccat cacctgtgtt ctctccttta tcaacatgtc ctgcaggcct gcacaaatcc
     2041 tctcttcccg catagggcat ttcaatctcg ataccgctcc tcttatgata gaagaacgcc
     2101 aaagattcat atttcatctg tgaataaata tccggacgga tatcaaaagg atggctataa
     2161 tttgttacag tttctacagt cggaagctca aatctatagc caattccttc agttgtaaag
     2221 tcagagaaat caagccaatg aacattatcc tgtgagtctt cgtcaagacc tatcggttct
     2281 gtataaccct catccacaac aacgcctgca gcatttaaaa gattccattt aacaggctgc
     2341 tttgaatcac aaactacggt agcaactttt ttaccttccg gtaaataacc tacctggtta
     2401 acacgtacat caggctgctt caattcttct ataacgggtt ttgtaaactc cggatcgtag
     2461 agggatacat catcaagaaa aaccctataa ggtacagtcc cagccttatc gcctcccaaa
     2521 tggaatgtaa attcacatgt gtcgtctgtt ggtgctgtca ttgtaaaggt ttctttaatt
     2581 tccaatacct cgcccggtgt aaggctatag gaagtccac
//
LOCUS       DAOOJC010000349         2618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_30572_length_2618_cov_4.196254, whole genome shotgun sequence.
ACCESSION   DAOOJC010000349 DAOOJC010000000
VERSION     DAOOJC010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30572_length_2618_cov_4.196254"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLA01_08795"
     CDS             complement(<1..405)
                     /locus_tag="PLA01_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPD01428.1"
                     /translation="MKKTISLLVISMLIIGILFPFTGNKFAFAEEGNQENQIFEDIKG
                     HWCEEFILKFYDRNWILGYDDGLFHPDKFVTRAEFTAMVVNIFKKRDITENNASEGKG
                     VFTDMSEKDWFYNAVKYAAEENLIKGFEDGTFK"
     gene            complement(803..1459)
                     /locus_tag="PLA01_08800"
     CDS             complement(803..1459)
                     /locus_tag="PLA01_08800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPD01429.1"
                     /translation="MAIMRVNPTRMELSRLKKRLQVARRGHKLLKDKLDELMKKFLDL
                     VRKNKELRETVEEMLMQVHQNFLIARAVMSSEGLEAALMLPKQSISLEVTTKNIMSVD
                     VPVLDYKTSNHDESSIYPYGFATTSGELDGAISALSKALPYMLELAQTEKSAQLLAQE
                     IEKTRRRVNALEYVMIPQLSETIKYITMKLDENERGNLTRLMKVKDMMIEQARKAATH
                     "
     gene            complement(1482..>2618)
                     /locus_tag="PLA01_08805"
     CDS             complement(1482..>2618)
                     /locus_tag="PLA01_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPD01430.1"
                     /translation="VSMDMLGRVFSGMGRPLDGGPEIIPEARLDINGTPINPAARDYP
                     SEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHAQLAAQIARQAKVLGTDSKFAVVFA
                     AVGITFEEANFFISDFKRTGAIDRTVLFINLANDPAIERISTPRMALTAAEFLAFEKD
                     MHVLVIITDITNYAEALREVSAARKEVPGRRGYPGYLYTDLATLYERAGRKLDSEGSI
                     TLIPILSMPEDDKTHPIPDLTGYITEGQIILGRELHRKGITPPIDVLPSLSRLKDKGI
                     GKGKTREDHADTMNQLFAAYARGKEAKELAVILGDAALTDTDKLYAKFADAFEKEYVS
                     QGFEEDRTIEKTLEIGWKLLSILPRGELKRIRDEYLDKYLPKGE"
BASE COUNT          665 a          597 c          536 g          820 t
ORIGIN      
        1 cttgaaggtt ccgtcttcaa aaccttttat aagattttct tctgcagcgt attttacggc
       61 attatagaac caatcttttt cactcatgtc agtgaaaaca cctttgccct cagatgcatt
      121 attttctgta atgtctcttt tcttgaatat gtttacgacc atagcagtaa actcagctct
      181 tgttacgaat ttatcgggat gaaacaatcc gtcatcgtaa cctaaaatcc agttgcggtc
      241 ataaaacttt aggataaatt cttcgcacca atgccccttt atgtcttcaa aaatttgatt
      301 ttcttgattg ccctcttcgg caaatgcgaa tttgtttcct gtaaaaggaa atagtattcc
      361 aatgatgagc attgaaataa caagtaaaga tattgttttt ttcatgataa acctccccta
      421 aaaaataaat aaaaatatat ataaagataa taattttcta ttctataata tttatgtaaa
      481 cactgtattt taaattatat ctaatttcaa gaacagaatc aacataaata ctgattggtt
      541 taataaaaag gttaatttta ataaaaaaca aacaaggtat gatttcattt tccatgtcct
      601 gcttgttttt tagtgggatt tcacgaagtg aaacgacaaa aaagagatgg tggttcgatg
      661 cccccatctc ttttgttctt ctatttaaac tttaataagc atccggcatg acacggaaga
      721 accgtccctc ccgtgtccta tttaaacttt aataagcatc tggcatgaca cggaagaacc
      781 gtccccctgt gctgcccatg tgttagtggg ttgcggcttt tcttgcctgt tctatcatca
      841 tgtctttgac cttcatcagc cttgtgaggt ttcctctttc attttcgtcc agcttcatgg
      901 ttatatactt gattgtttcg gaaagctggg gtatcatgac gtactctaaa gcgttaaccc
      961 ttcttcttgt tttctcgatt tcctgagcaa gaagctgggc cgatttttcg gtctgggcaa
     1021 gctcaagcat gtagggaagt gcttttgaca gggctgagat tgcaccgtca agctccccgg
     1081 aggttgttgc aaagccgtag ggatatatgc tgctttcatc gtggttggag gttttatagt
     1141 ccagtaccgg tacatctacg ctcattatgt ttttggttgt tacttccaaa gatatgctct
     1201 gctttggaag catgagggca gcctcaagtc cttccgaaga cataacagcc ctggctatga
     1261 gaaagttctg atgtacctgc attaacattt cttcaaccgt ttctcggagt tccttgtttt
     1321 tcctcaccag atcaagaaac ttcttcataa gctcatccag cttatcttta agaagcttat
     1381 gccccctgcg ggcaacctgc agacgtttct tgaggcggga gagctccata cgggtcggat
     1441 ttacacgcat aattgccata gtatgcttct ctccttataa atcattctcc tttgggaagg
     1501 tacttgtcaa ggtactcatc cctgatacgt ttaagctctc ctcgaggcag gattgaaaga
     1561 agcttccaac ctatctcaag ggttttttca atggttctgt cttcttcaaa cccttgggaa
     1621 acatattcct tttcaaaagc atccgcaaac tttgcataca gcttgtcggt gtcggtaagg
     1681 gcagcatcac ccagaataac tgccagctct tttgcttctt taccccttgc ataggcggca
     1741 aacagctggt tcattgtgtc ggcatggtcc tcgcgggttt taccctttcc gataccctta
     1801 tccttcaaac gggacaggga aggcaaaaca tctatcggcg gagttatacc ttttctgtgc
     1861 agttctcttc ccaaaattat ctgaccctcg gttatatagc ccgtaaggtc ggggatggga
     1921 tgagttttgt catcctccgg cattgaaagt atgggaataa gggttatact tccttcggag
     1981 tccagctttc ttcccgctct ttcatacaag gttgcaaggt ctgtataaag gtaaccggga
     2041 taacctcttc ttccgggaac ctcttttctg gccgcagaaa cctcacgcag tgcctcggcg
     2101 tagttggtta tatcggtaat gattaccagt acatgcatgt ctttttcgaa ggccaggaac
     2161 tctgcagcag tcaatgccat acgaggcgta gatatacgct caatggccgg gtcgttggcc
     2221 aggtttataa acagaacggt acggtctatg gcaccggttc ttttaaagtc gcttatgaag
     2281 aagttcgctt cctcaaatgt aatacctact gccgcaaaca caacggcaaa cttgctgtcg
     2341 gtacccaaaa cctttgcctg ccttgcaatt tgggctgcaa gctgggcgtg gggaagaccg
     2401 gagccggaga atatgggcag cttttgtcca cgaaccagag tgttcagacc gtcaattgcc
     2461 gaaactcctg tctgtataaa ctcagaagga tagtccctgg ctgcaggatt tataggggta
     2521 ccgtttatat caagccttgc ttcgggtata atttccggtc cgccgtctag gggccggccc
     2581 attccgctga atacccttcc caacatatcc attgacac
//
LOCUS       DAOOJC010000350         2601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_30863_length_2601_cov_3.724666, whole genome shotgun sequence.
ACCESSION   DAOOJC010000350 DAOOJC010000000
VERSION     DAOOJC010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30863_length_2601_cov_3.724666"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(736..2271)
                     /gene="guaA"
                     /locus_tag="PLA01_08810"
     CDS             complement(736..2271)
                     /gene="guaA"
                     /locus_tag="PLA01_08810"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPD01431.1"
                     /translation="MNNELILVLDFGGQYNRLIARRVRDANVYCEVIPYSTSIDKIKK
                     LNPKGIILTGGPASVLDPKAPICDRGVFELGVPVLGICYGMQLMSYVLGGSVESAEQR
                     EYGKVNISLDTANKLFEGIDGETTCWMSHTYYVNNLPQGFVKSAYTMNCPVGAMENRD
                     KKLYGVQFHPEVVHTPQGTDILKNFLYKICGCSGDWRVSSFIEHSINSLKEKIGDKKV
                     LCALSGGVDSSVAAVMIHKAVGKQLTCIFVDHGLLRKYEADQVEEVFKTQFDINLIRV
                     DAERRFLERLKGVTEPERKRKIIGEEFIRVFEAEAKKIGAVDFLVQGTIYPDVIESGV
                     GDAAVIKSHHNVGGLPEHVDFKEIIEPLRNLFKDEVRKVGLELGLPEEIVMRQPFPGP
                     GLAIRVIGEVTKEKLDILRDTDYIFREEIKNAGLATEINQYFTVLTGMRSVGVMGDER
                     TYDYTLALRAVTTTDFMTADWARIPYDVLEKVSNRIVNEVGHINRIVYDITSKPPATI
                     EWE"
     gene            complement(2359..>2601)
                     /locus_tag="PLA01_08815"
     CDS             complement(2359..>2601)
                     /locus_tag="PLA01_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPD01432.1"
                     /translation="PAKAANAGGVATSGLEMCQNSMRYSWTFEEVDEKLKHIMIDIFK
                     SASEAAKKYGFENNLVVGANIAGFLKVAEAMMAQGI"
BASE COUNT          682 a          694 c          450 g          775 t
ORIGIN      
        1 gaccccgcct ttctggttcg tgatggcaat cacatgacag ttgtccatgg ggattcctcc
       61 tttccttgtt tagccggatg agtccggcat gtaaggaccg cagtatttgt tctcaacagc
      121 ccctgcggta aaagcgatgt agcttaaggg aaatcatcgg gcggctgtgt gcttcacagc
      181 ctcataggag tctcacctct gccgggttct ccgccagctc cgtagagaag tatcattatc
      241 gtcagacctg tcatcgcctg ggagagtagc agtctctcca ttgcggcact ggattctcgc
      301 gactctcaaa gagctaagcg tacatgccgt atggctcatc agtctgatta acgtacctga
      361 tgatctgtat attcggtttt caagcagcgc gaagtggcgc tccttgtcgg gaggagaacc
      421 ccttcattag gtaatgggaa taactcgacc attttttagg gttcctccaa aaaatttttt
      481 tggcataaaa aaagagccag tccgtcgtca aaacgaactg gcttaaatag ctatttatat
      541 acgtttttag gcctttcgcg gttagccact ggctactatt aaaatcgtct ctgtgcaacc
      601 aaagggcctt ttcccagctc tcgcggttgc acggcggttg cacggcacca ttcccgctaa
      661 ctcgtcaaaa cccggaattg cctgtaatta caggggtttt cggcgttttt tgtgcaacca
      721 gcaagccttt acgttctact cccactcaat tgttgccggc ggcttgcttg ttatgtcata
      781 cacaattctg ttaatatgcc ctacttcatt tacaatcctg tttgatacct tttcgagtac
      841 atcataagga attcttgccc agtccgccgt cataaaatca gtagtggtaa ccgcccttaa
      901 tgcaagagta tagtcgtatg ttctttcgtc acccataaca cctacactcc tcattccagt
      961 cagcaccgta aaatactggt ttatctccgt tgccaacccg gcattcttaa tctcttcacg
     1021 gaaaatgtaa tccgtatccc ttagaatatc caacttctct ttagtaacct ctccgataac
     1081 ccttatggca agacccggtc ccgggaaagg ctgcctcata actatctcct caggcaatcc
     1141 cagctccaga cctacctttc tgacctcatc cttaaacagg tttctcaaag gctctattat
     1201 ctccttaaag tccacatgtt ccggaagtcc gccgacgttg tgatggcttt ttataacagc
     1261 cgcatctccg actccgcttt caattacatc gggatatatg gtgccctgaa ccaagaaatc
     1321 tacagcaccg attttcttgg cttccgcttc aaacaccctg ataaattcct cgcctattat
     1381 cttccttttt ctttcaggct cagtaactcc tttaagcctt tcaaggaacc ttcgctccgc
     1441 atcaaccctt atcagattta tgtcaaactg tgttttaaac acctcttcaa cctgatctgc
     1501 ttcatacttt cttaaaagac catggtcgac aaaaatgcat gtcagctgtt ttcctactgc
     1561 cttatgaatc ataactgcag ccacagaaga atcaacaccg ccggataggg cacaaagaac
     1621 ctttttgtct ccaatcttct cctttaaaga attgatagaa tgttcaataa aggaacttac
     1681 tctccaatcc ccggaacaac cgcatatctt ataaaggaaa ttcttaagaa tatccgtgcc
     1741 ttgtggtgta tgaaccactt caggatggaa ctgaacccca tatagcttct tgtctctgtt
     1801 ctccattgca cctacaggac aattcatcgt ataagcactc ttaacaaacc cctgtggaag
     1861 gttatttaca taataagtgt gactcatcca gcaggtggtt tctccgtcaa ttccttcaaa
     1921 cagtttattt gcagtgtcca gactaatatt caccttgccg tattctctct gttccgcact
     1981 ctctacactt ccgccaagca cataactcat taactgcatg ccatagcaaa tacccagaac
     2041 ggggaccccc agttcaaaca ccccacggtc acaaataggt gccttaggat ccagaaccga
     2101 tgcaggtccc ccggttaaaa taatcccctt tgggttcaac ttttttatct tatctataga
     2161 agtgctgtat ggaataacct cacagtatac atttgcatcc cttactcttc ttgcaatcag
     2221 ccgattatac tgtcctccaa aatcaagaac taaaatcaat tcattgttca aactaacatc
     2281 tccttcaaac ataaatatga tgtttaatta tatcgaaaat cggcatacaa tgcaaaaaaa
     2341 attttaaaaa ttttatatct atattccctg tgccatcata gcctctgcaa ccttcaaaaa
     2401 tcctgcaata tttgcaccta cgaccaggtt attttcaaac ccgtacttct ttgcagcttc
     2461 actggcactc ttaaatatat caatcattat atgctttaat ttttcatcga cttcttcgaa
     2521 cgtccatgaa tacctcatgc tgttttgaca catttcaagt ccggaagtag caacaccgcc
     2581 ggcatttgca gcctttgcag g
//
LOCUS       DAOOJC010000351         2591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_31031_length_2591_cov_4.019716, whole genome shotgun sequence.
ACCESSION   DAOOJC010000351 DAOOJC010000000
VERSION     DAOOJC010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31031_length_2591_cov_4.019716"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1932)
                     /locus_tag="PLA01_08820"
     CDS             complement(<1..1932)
                     /locus_tag="PLA01_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPD01433.1"
                     /translation="MRSKRFIDFLTKIILAGFMSFSLVYPMTTTLLFPYSALSIMGYV
                     FIALMIYSVMFINRLSAKVSAILVLAASGAGIFLLATKQKLHHIVGIVVWLVKYISNG
                     TELIDNYAFILTLFFCIAFSLFVFIFTIKKFNFYIIALSGIIIFSSQWMLDFFVDKAY
                     ISFYTFIVSIAVYYLMHIYSNKIIDDSSDLVPPSAFILGIVPLCILVLLLTNSIPARS
                     KPLEWKWLDQQINFAFNYLRSDKKTGSAVYDLGYFSLKTTGFANGNANLGGNITPNDM
                     KIMEVQTTQKIYLRGNAYNIYTGNSWISTPPIEGFNSLQTVRGNAPIMKADGTLINVP
                     DVIIDESMDILELEKGAIALYEYEPSDGSYSNQDEPWPMDFDNILNFAYDGNILSKLT
                     NIAKVKVKFSDIKANTIFAPLKSERFQYFSDISGNIDRNAEDIFTSTESLEKDFSYYF
                     ESYCPKVGNSFFQSVLRKSKRGFYIDEIDRQTNEIYKLLYDLYLDDLIVKIGRETSIN
                     VWDSSNEAAVKAIIRNSSDKYTLGDELFKYLTNYFSRDIYNPIDMDISIYFLKSIGEF
                     REIPPVNDFNDFKKLNEYSNTIYQRYLDIPPSVPQSVKDLAVSITKDETTDFDKAKAI
                     EKYLSSNYPYSLTPGDTPPD"
     gene            complement(1951..>2591)
                     /locus_tag="PLA01_08825"
     CDS             complement(1951..>2591)
                     /locus_tag="PLA01_08825"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPD01434.1"
                     /translation="VSDIRKYVIGDSLRKIHWKLSSKMNELLVKNYESASSTMSAIIL
                     DLKKNNYTVEQNAKIEDNLIESSLAVIYYCLNNYIPINLSYYSKGFVDIEANKPLHFE
                     GIYSTLSKIKFDHDVDVKDVLDIYTGKNSLIRTNIMLFTSNLSFDLYDKLYKAKLSEY
                     DVNLIYIAPNVALDSDKAEIDNILKELPEMGITTYRINPEDNIKNVLETLLN"
BASE COUNT          798 a          446 c          492 g          855 t
ORIGIN      
        1 atccggaggt gtatctcctg gggtcaggga ataaggatag tttgatgaca ggtatttttc
       61 aattgctttt gctttatcaa aatctgtggt ttcgtcttta gttatggata ccgccagatc
      121 ttttacactt tgaggaacac tggggggtat atcaagatat ctttgataga ttgtatttga
      181 gtattcgttc agctttttaa aatcgttaaa atcgttaacg ggaggaattt ccctgaattc
      241 gccgatgctt tttagaaagt atattgagat gtccatatca atgggattgt aaatatctcg
      301 tgaaaagtag tttgtcaggt atttgaacag ttcatctcca agagtgtatt tatccgagga
      361 atttctgata atagctttta ccgccgcttc gtttgaagag tcccacacat ttatagaggt
      421 ttcccggccg atttttacaa tgagatcatc aaggtagaga tcgtataata atttataaat
      481 ttcgttggtt tgcctgtcaa tttcatcaat atagaatcca cgcttgcttt ttcttaaaac
      541 gctttggaaa aatgaattgc caactttggg acaataactc tcaaaataat aactaaaatc
      601 tttctccaga gattctgttg aggtgaatat atcctccgca tttctgtcaa tattgccgct
      661 tatatccgaa aagtattgga atctttccga cttgagaggt gcaaatatag tgtttgcttt
      721 tatgtccgaa aatttaactt taactttagc tatattcgta agtttggaaa gaatatttcc
      781 atcatacgca aaattaagta tgttatcaaa atccatcggc caaggttcgt cttgattgga
      841 ataggaacca tctgagggtt catactcata aagagcaatt gccccttttt ccaattccaa
      901 tatatccatg ctctcgtcta tgatgacatc ggggacgttt atgagtgtac cgtctgcttt
      961 cattattgga gcattgcccc taactgtttg cagagagtta aacccttcta ttggtggagt
     1021 gcttatccat gaattaccgg tgtagatatt gtaagcatta ccacgaaggt atattttttg
     1081 agtggtttga acctccataa tcttcatgtc gttgggggtt atatttccgc ccaggttagc
     1141 gttaccattg gcaaatccgg tggttttaag agaaaagtaa ccaaggtcgt aaacagcgga
     1201 acctgttttt ttgtcagatc gaaggtaatt aaaagcaaaa ttgatttgct ggtccagcca
     1261 cttccactct aaaggttttg accttgcggg gatggagttg gttaacagga gaaccagaat
     1321 acataaagga acaataccca ggataaaagc cgagggcgga accagatcgc ttgaatcgtc
     1381 aataatttta ttgctgtata tatgcatgag gtaatatact gcaattgaaa caataaaggt
     1441 atagaacgat atatatgcct tgtcaacaaa aaaatcaagc atccattggg atgagaatat
     1501 tataattccg ctcaatgcaa ttatataaaa gttgaatttt tttattgtga aaataaacac
     1561 aaatagtgag aaggctatgc aaaagaaaag ggtgaggata aaagcataat tgtctatgag
     1621 ttcagtaccg ttactgatat atttaaccag ccatacgaca ataccgacaa tatggtgcag
     1681 cttttgcttg gtagctaaaa gaaatattcc cgctccggat gctgccaata ccaatatggc
     1741 ggatactttt gccgataacc tgtttataaa cataaccgaa tatatcataa gggctatgaa
     1801 aacataaccc atgatactca aagcagagta aggaaacagc agggtggttg tcatgggata
     1861 caccaggctg aagctcataa acccagcaag aattattttg gttaaaaaat cgatgaatct
     1921 tttcgatctc atgttaattt cacctcatat tcaattcagt aaggtctcca agacattttt
     1981 tatgttatct tccgggttga ttctataagt tgttataccc atttcaggga gttcttttaa
     2041 aatattgtcg atctcagctt tatccgaatc caaagcaaca ttgggtgcaa tatatatcaa
     2101 atttacatcg tattctgaaa gctttgcctt gtataactta tcatacaggt caaagctcag
     2161 atttgatgtg aaaagcatta tatttgtcct aataaggctg tttttgcctg tatagatatc
     2221 aagaacatcc tttacatcaa catcatggtc aaatttaatt ttcgaaagtg tgctgtagat
     2281 tccttcgaaa tgcagcggtt tattggcttc aatatccaca aagcctttac tgtagtatga
     2341 aaggtttatt ggaatataat tgttaaggca ataatatata actgctaaag aggactctat
     2401 cagattgtct tcaatttttg cattttgttc aactgtataa ttgttctttt ttaaatccaa
     2461 aataatagca ctcatagtac tggaagcact ttcataattt tttacaagaa gttcattcat
     2521 tttagaagaa agcttccagt gtatttttct gaggctgtcc ccgatgacgt acttcctgat
     2581 atccgaaaca g
//
LOCUS       DAOOJC010000352         2587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_31112_length_2587_cov_4.511848, whole genome shotgun sequence.
ACCESSION   DAOOJC010000352 DAOOJC010000000
VERSION     DAOOJC010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31112_length_2587_cov_4.511848"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2298
                     /locus_tag="PLA01_08830"
     CDS             <1..2298
                     /locus_tag="PLA01_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HPD01435.1"
                     /translation="YRDARLRRITRYRYNNVPIDMGGRYFYIFDGGDFWSPGWSPVKN
                     KLDSYECRHGLGYTRITGKKNGISTEVTFFVPQNYNGEVQKVVIKNEGSQNKKITFFS
                     FLEFCLWNAYDDMTNFQRNFSTGEVEIEGSVIYHKTEYKERRNHYAFYSVNEKITGFD
                     TDRDSFIGLYNGFEAPQAVVAGKSSNSVADGWSPVASHSIEIELKPGEQKDLVFILGY
                     VENKFEEKWESKGVINKKKAHEMIDKFKTSADVDRAFNELKDYWTSLLNKYTIKSHDE
                     KLDRMVNIWNQYQCMVTFNMSRSASYFESGIGRGMGFRDSNQDLLGFVHQIPERARER
                     LLDLAATQLEDGGAYHQYQPLTKKGNNEIGSNFNDDPLWLILATAAYIKETGDYSILD
                     EQVPFDNDESKADTMFEHLRRSFYHVVNNLGPHGLPLIGRADWNDCLNLNCFSSVPDE
                     PFQTTTSKDGKVAESVMIAGMFVFIGADYVKLCQYKGLDEQAKEAQAHIDKMKETVMK
                     HGYDGEWFLRAYDDFGKKIGSKENEEGKIFIESQGFCVMAGIGKEEGSAQKALDSVKK
                     YLDTPYGLVLQNPAFTKYYIEYGEISTYPAGYKENAGIFCHNNAWIICAEAELGRGDL
                     AFEYYSKIAPAYTEHISEIHKLEPYVYAQMIAGKDAKRHGEAKNSWLTGTAAWNFVAI
                     SQWILGIIPDYNGLKVDPCIPKDWDGYTITRWYRGSNYIISVKNPDHVSKGVKKVVVD
                     GKEISGNILPVFNDGKDHNVEIIMG"
     gene            2311..>2587
                     /locus_tag="PLA01_08835"
     CDS             2311..>2587
                     /locus_tag="PLA01_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01436.1"
                     /translation="MFEKLAARGIKTTPEAVLKSSFASVLKEQWINDAMFLDTALNRV
                     IGVANLPFKAPAVLQLLKNRDVAAYLIELAGDDSSVMVIKEENGVKVF"
BASE COUNT          829 a          464 c          648 g          646 t
ORIGIN      
        1 tacagggatg caaggctgag acgtattaca aggtacagat acaataatgt gcctattgac
       61 atgggtggac gttattttta catatttgac ggaggggatt tctggtctcc gggatggtca
      121 cccgtgaaaa ataagcttga cagctatgag tgcagacacg gattgggcta tacaagaatc
      181 acaggaaaga aaaacggaat tagcacggag gttaccttct ttgtacctca aaactataac
      241 ggtgaagttc aaaaggttgt tattaagaac gagggttccc aaaataaaaa gataacattc
      301 ttctcattcc tggaattctg tctgtggaat gcttatgacg atatgaccaa tttccagaga
      361 aatttcagca ccggagaggt tgagatcgag ggttcggtta tataccataa gacagagtat
      421 aaggaacgca gaaaccacta tgcattctat tctgtaaatg aaaaaatcac tggatttgac
      481 actgacaggg acagctttat cggtttgtac aacggttttg aagctcccca ggcagtggtt
      541 gctggaaaat caagcaattc ggttgccgac ggttggtcac cggtagcatc ccacagtatt
      601 gaaatagagc ttaagccggg agaacaaaaa gacctggtat ttattctggg ctatgttgag
      661 aacaaatttg aagaaaaatg ggagtcaaag ggcgtaatca ataagaaaaa ggctcatgag
      721 atgatagaca aattcaagac ttccgcagat gttgaccgtg cttttaatga gcttaaagat
      781 tattggacat cccttttaaa caaatacaca atcaagagcc atgatgagaa gctcgacaga
      841 atggtaaaca tctggaacca gtatcagtgt atggttacct tcaatatgtc tagaagtgcg
      901 tcatattttg aatccggtat aggaagagga atgggcttca gggattccaa ccaggacctt
      961 ttgggattcg tacatcagat tccggaaaga gcaagggaaa gacttttgga tcttgcggct
     1021 acacagcttg aggatggtgg agcttatcac cagtatcagc cgctgaccaa aaaaggaaat
     1081 aatgaaatag gcagtaactt taacgatgac ccattgtggc tcattttggc tactgcagca
     1141 tacatcaaag aaacagggga ctatagcata ttggacgagc aggtaccctt tgacaatgat
     1201 gagagtaaag cagatacaat gtttgagcat ttgaggcgtt ccttctacca tgttgtcaac
     1261 aatcttggac ctcatggatt gcctcttatc ggaagggcag actggaatga ctgcttgaat
     1321 ctgaattgct tctcctccgt accggacgag cctttccaga ccaccacaag caaggacggc
     1381 aaggtggcag agtcggttat gattgccggt atgtttgtat tcatcggagc ggactatgtg
     1441 aagctttgcc aatacaaggg attggatgag caagcaaagg aagcacaggc acatattgac
     1501 aaaatgaaag aaacggttat gaagcatggc tatgacggag agtggttctt gagagcctat
     1561 gacgatttcg gaaagaaaat cggaagcaag gaaaacgaag agggcaagat atttatcgaa
     1621 tctcagggct tctgtgtaat ggcaggtatc ggaaaggaag agggaagtgc ccaaaaggct
     1681 ttggattcag ttaaaaagta tcttgacact ccatacggac ttgtgcttca aaaccctgca
     1741 tttacaaagt attatataga atatggtgaa atatccacgt atcccgcagg atacaaggaa
     1801 aatgccggaa tattctgcca taacaacgca tggataatct gtgccgaggc tgaattggga
     1861 agaggagatc tggcatttga atactattct aaaattgcac cggcgtatac cgagcacatc
     1921 agcgaaatcc acaaattaga gccctatgta tacgcacaga tgattgccgg aaaggacgca
     1981 aagaggcatg gggaagctaa gaactcatgg cttaccggaa cagctgcctg gaactttgtt
     2041 gcaatttccc agtggattct gggtatcata ccggattaca acggtcttaa agtagatcct
     2101 tgcattccga aggattggga cggatatact attacccgtt ggtacagggg ttcaaactat
     2161 attatcagtg ttaaaaaccc tgaccatgtt tccaaaggag ttaagaaggt agtagttgac
     2221 ggaaaagaaa tatccggaaa tattctgccg gtatttaacg acggtaaaga ccacaatgtt
     2281 gaaataataa tgggctaggg ggaagtaaaa atgtttgaga agctggcagc aagaggtata
     2341 aaaacgacac cggaagcagt gcttaaaagc agctttgcca gtgttctgaa agagcaatgg
     2401 attaatgacg caatgttttt ggacactgct ttaaacaggg tcataggggt tgccaatttg
     2461 ccttttaaag cccctgcggt tctgcagctt ttaaaaaaca gggatgttgc ggcatacctt
     2521 atagaattgg caggcgatga cagctctgtt atggtgataa aggaagaaaa tggtgtcaag
     2581 gtttttg
//
LOCUS       DAOOJC010000353         2584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_31158_length_2584_cov_3.658758, whole genome shotgun sequence.
ACCESSION   DAOOJC010000353 DAOOJC010000000
VERSION     DAOOJC010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31158_length_2584_cov_3.658758"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="PLA01_08840"
     CDS             <1..1475
                     /locus_tag="PLA01_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPD01437.1"
                     /translation="LFIGLKNEVLINVYKDVDIGGNSEIFVIDKEGIIISSEDEKELG
                     NMYGDEKLVNEIGAKISEGVNVFEFNDYLVSYKEIEGTNWVVVGKIPISYTRHEPERI
                     RNSLLAFIAVCLILSLLASIIISMSISVPLEKMAKLMNETKNGIFTSDIEDCGNDEIS
                     EVIKDFKNMILNISVLISKVKLSSDDVLNHLSVLDTSIEKTNVASKQINSITHQIAAD
                     ASEQASEINNCALSIRVLSEGINKVEDNMQDMTKVIENTKNLSHDALGIVETLNDKAT
                     KTSMASNRVIEDINKLSDDIKRIKAIISTIVGIAEQTNLLSLNAAIESAKAGEAGKGF
                     GVVAGEIKALAEQSKEASSKISRIINDILVKTEGTVNVAYSAHSNVNDQMLVVNQTNE
                     SFNSIFNAMESIVDCIGNMSESIKGVLDSKEKVSASIENIAAISEQTASITQEVSATM
                     QEQYTYMESLAVSSKKINMMAEQLGDAVSKFRIRNEEDKR"
     gene            1472..>2584
                     /locus_tag="PLA01_08845"
     CDS             1472..>2584
                     /locus_tag="PLA01_08845"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPD01438.1"
                     /translation="MKKVFAKIVCMVIMLSLFLSLFACANSGKAKDVDEEPNSQTNSK
                     DNGDKIKLSLWHMWVSDSDGNKKPFQTVLKHVEEKFPNVELAVEDIDNESYKKKLKNA
                     MATNQLPDIFFTWSAGFSKPFVESGKVLALDEYIKDVKDKLLPGITDSMTYGGKVYGL
                     PFQMQVAALYCNQELFEQNGVKIPENYEELLTAVKAFSHKGITPMVNGAKDLWPAMFY
                     YDILALRTAGPQLCNDALNKNASFDSPEFIDAARRLVELTKAGAFGENPLGLSYDNGN
                     IMFAEGKAAMLFNGNWVAGIVEGDSSKVKGKIVAVKFPLIDSGKGSFTDYLGGAGDGF
                     MVSADTPDKELAVKVAKFIAEDMAREAYLANAGVPGW"
BASE COUNT          883 a          396 c          598 g          707 t
ORIGIN      
        1 acttgtttat cgggctgaaa aacgaagtat tgattaatgt ttataaagat gttgatatag
       61 gaggaaattc ggagattttt gttattgata aagaaggaat aattatttca tctgaagatg
      121 agaaagaact tggaaatatg tacggtgacg aaaaacttgt gaatgaaatc ggtgcaaaga
      181 taagtgaggg tgtaaatgta tttgagttta atgattactt ggtgtcgtat aaagaaattg
      241 aaggaacaaa ttgggttgta gtcggcaaaa ttcctatatc atataccagg catgagcctg
      301 agaggatcag aaactcactg cttgcattta tcgcagtatg tctgattctt tccttattgg
      361 catcgattat tatatcgatg agtatttccg tgccccttga aaaaatggcc aaacttatga
      421 atgaaactaa aaacggtatt tttacatccg acattgagga ctgcggaaat gatgaaattt
      481 cggaagtaat aaaagatttc aagaacatga tattaaatat aagtgttctt atatccaagg
      541 tcaagttatc atccgatgat gtattgaatc atttgtccgt attggacacc tcaattgaaa
      601 aaaccaatgt tgcttcaaag caaataaaca gtattaccca tcaaatagct gccgatgcat
      661 cggaacaggc ttctgaaata aacaactgtg cattaagtat cagggttctt tcagaaggca
      721 ttaacaaggt tgaggataac atgcaggaca tgacgaaggt aatagagaac accaaaaatc
      781 tcagccatga tgccttggga attgtcgaga cattaaatga taaagcgaca aagacaagta
      841 tggcatcaaa cagagtaata gaggatatca ataaactcag tgatgatata aaaagaatca
      901 aagcgataat ttcaaccatt gtgggaattg cagagcagac aaatctcctt tctctgaatg
      961 ctgctataga atctgcaaag gccggtgagg cagggaaagg tttcggagtg gttgcaggcg
     1021 aaatcaaagc acttgccgag cagtcaaaag aggcttccag taagattagc aggattataa
     1081 acgatatttt ggtgaaaacg gaaggaacgg taaatgtagc ttacagtgca cacagcaatg
     1141 taaatgatca aatgttggtt gttaaccaaa ccaatgaatc gtttaattcc atatttaatg
     1201 caatggaaag tatagttgat tgcataggca atatgtcgga gtcgattaaa ggagttcttg
     1261 attcaaaaga aaaggtgtcg gcatccatag aaaatatagc tgcaatttca gagcaaacgg
     1321 catcaattac acaagaggta tctgcaacaa tgcaagaaca atacacatat atggaatctc
     1381 ttgctgtatc ttctaaaaaa ataaatatga tggcagaaca attaggggat gcagtatcaa
     1441 aattcagaat aagaaacgag gaggataaga gatgaaaaaa gtgtttgcta aaatagtttg
     1501 catggtgatt atgctgtctt tatttcttag tttgttcgca tgtgcaaaca gtggaaaagc
     1561 caaggatgtt gatgaggaac caaattctca aacaaacagc aaagataatg gggataaaat
     1621 aaaactcagt ctttggcata tgtgggtttc tgacagtgat ggaaataaaa agccttttca
     1681 aacggtattg aaacatgttg aagaaaagtt tccaaatgtc gagcttgcgg tagaggacat
     1741 tgataacgag tcttacaaga aaaaattaaa aaatgctatg gcaacgaatc agcttcctga
     1801 tattttcttt acttggtctg ccggcttttc gaaaccgttt gttgaatctg ggaaagtgtt
     1861 ggctcttgat gaatacatta aagatgttaa ggacaagctt ttgcccggaa taacagacag
     1921 tatgacatac ggcggcaagg tatacgggtt gccgtttcag atgcaggttg ctgctctata
     1981 ctgtaatcag gaactgtttg aacagaacgg agttaaaatt ccggaaaact atgaagagct
     2041 gttaacggcg gtaaaagcat tttcccataa agggattacc cctatggtaa acggggctaa
     2101 agacttgtgg cctgcaatgt tttattatga tatcttggct cttcgaacag caggaccaca
     2161 attgtgtaat gatgcattga acaaaaatgc atcctttgat tctcctgaat ttattgatgc
     2221 ggcaagaagg ttggtcgaat taacaaaagc cggtgctttc ggtgaaaatc cccttggctt
     2281 gtcctatgat aacggtaaca taatgtttgc agaaggaaaa gctgcgatgc tgttcaacgg
     2341 taactgggtt gccggtatag ttgaaggtga ttcctcaaaa gtcaagggta agattgttgc
     2401 ggttaaattc ccattgattg acagtggaaa aggaagtttc acagattatt tgggaggtgc
     2461 cggtgacgga tttatggtta gtgccgacac acccgataag gaactcgccg ttaaggtggc
     2521 aaagtttata gcagaagaca tggcaagaga ggcttatctt gcaaacgcag gtgtgccggg
     2581 ctgg
//
LOCUS       DAOOJC010000354         2584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_31164_length_2584_cov_2.870304, whole genome shotgun sequence.
ACCESSION   DAOOJC010000354 DAOOJC010000000
VERSION     DAOOJC010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31164_length_2584_cov_2.870304"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PLA01_08850"
     CDS             <1..683
                     /locus_tag="PLA01_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin"
                     /protein_id="HPD01439.1"
                     /translation="PIPTPTPTATQSYSYNDGYFPAGTSKSELISKASTLSVSEVKAI
                     IKKQVDDHWDVIRNVCGFKNKEAAYAFFFGMATRESTFRAATETGGGAAHAFGPLQTA
                     ETAYANANPDYMPELNVPEMTQYDFNDYNFYDVGVSTHMGIRHFLHFARLAQEKYSGK
                     DILRHGLMGYNTGWIDGSSEAWIIQYADETAALGAWYYQNNHMSDNEFTWDTDPRVDR
                     SNPWSLYF"
     gene            complement(969..1715)
                     /locus_tag="PLA01_08855"
     CDS             complement(969..1715)
                     /locus_tag="PLA01_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HPD01440.1"
                     /translation="MYELNICHLYPDLLNLYGDRGNIIALKRRCEWRGINVNISNISI
                     GDMFDAESYDIIFLGGGQNYEQEILHDDIINEKGNEIRNAINNDKVFLAVCGGYQLLG
                     KFYKTWDGKEIQFLDVIDFWTIGDKKRMIGDFVFECDFLMKNGFDGRVVGFENHSGRT
                     YLGTGVKPLGKIIKGFGNNGKDGFEGALYKNVYCTYSHGSLLPKNPVFADHLIALSLK
                     QKYKDFVSLSCLKDEFEELANRSMIKRILG"
     gene            complement(1717..>2584)
                     /locus_tag="PLA01_08860"
     CDS             complement(1717..>2584)
                     /locus_tag="PLA01_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1727 domain-containing protein"
                     /protein_id="HPD01441.1"
                     /translation="HNVKRDVIYYGFAQGASDSGEKVENSDAVYCLYCKNRYEYSYQV
                     YGHLGAFSCPNCGYSRPSPQVTCLGVNDLNSSYSDIVFSLENAGDDSKKPSFTARINL
                     PGLYNIYNALAAAALGHLLGFPADSIIKAMESFECGFGRMETLVTNGKTIKIILVKNP
                     TGFNQVLTYLLTETQKAQIAFIINDKLADGTDISWLWDVYFEKLENIQENISLFFASG
                     IRAEDMAVRLKYAGIYSDKISLIKDYEQLLNKALDDTQEGKTLYILPTYTAMLDIRKL
                     LNKKYGLKEFWK"
BASE COUNT          762 a          536 c          509 g          777 t
ORIGIN      
        1 cacctatacc cacccctaca cctacagcta cacaatccta tagttataat gacggttatt
       61 ttccggccgg gacttcaaaa tctgaactta tttcaaaggc atctacattg agtgtaagcg
      121 aagtaaaggc tatcataaag aaacaggtgg atgatcactg ggacgtaata aggaatgtat
      181 gcgggttcaa aaacaaagag gcggcatatg catttttctt tggaatggct acccgtgagt
      241 ctacattcag ggctgcaaca gagacaggag gtggagcggc acatgctttc ggacctttgc
      301 agactgccga aacggcttat gccaatgcaa atcctgacta tatgcccgag ttgaatgttc
      361 ccgaaatgac acagtacgat tttaatgatt ataatttcta tgatgtcggt gtatctacac
      421 atatgggaat aagacacttt ttacattttg cccgtctggc acaggaaaaa tacagcggca
      481 aggatatact tcgtcacggt ttgatgggat ataacaccgg atggatagat ggttcgagtg
      541 aagcatggat tatacagtat gctgatgaaa cagctgcttt aggtgcatgg tattatcaaa
      601 acaatcacat gtctgataat gaatttacgt gggatacgga tccgagagta gatcgcagca
      661 acccatggag cttgtacttc taaggaattt taagaagggg agctaaggca ttaagccgaa
      721 tgggtatata ctgttaagta aattaaaata tgaattaatc attgtgaagc aaatagcagg
      781 agcctgtaaa taccatgggg aaaggaagag caatcattcc cgcaaagcca gttttattcg
      841 accccacgga gtatttacag gctctttaat tgagaaactt aagttttctc ctatattttt
      901 ctatacattt acacctttat ttcatacacg ggaatagaac acaaaagatt caggatattg
      961 agcctgactc atcctaaaat tcttttaatc atggagcggt tggccaattc ttcaaattca
     1021 tccttgagac acgaaagtga cacaaaatcc ttgtattttt gcttaagtga cagtgctata
     1081 aggtgatctg caaacacagg attcttagga agaaggctcc catgggaata ggtacagtaa
     1141 acatttttat ataatgctcc ctcaaaacca tccttaccgt tgttgccaaa tcccttaatg
     1201 attttgccca agggtttgac accggtacct aaataggttc tcccggaatg gttttcaaag
     1261 cccacaaccc ttccgtcaaa gccgtttttc ataaggaagt cgcattcgaa gacaaaatct
     1321 ccaatcattc tctttttatc gcctattgtc cagaaatcta tcacatccag aaattgtatt
     1381 tctttgccgt cccaggtttt atagaatttt ccgagaagtt gataaccgcc gcagacagct
     1441 aaaaacacct tgtcgttgtt tatggcattg cggatttcat ttcccttctc atttataata
     1501 tcatcatgaa gtatttcctg ttcgtaattc tgacctccac ccaggaaaat aatgtcgtag
     1561 ctttcggcat cgaacatgtc accgatggaa atgttcgaaa tgttaacgtt tattcccctc
     1621 cattcgcacc gtctcttcaa ggctattata tttccccggt cgccgtacag attcaataag
     1681 tcggggtaaa gatggcatat atttagttcg tacatattat ttccagaact cctttaatcc
     1741 atattttttg ttaagcagtt ttctaatgtc cagcattgcc gtgtatgtgg gtaatatata
     1801 aagggtttta ccctcctgcg tgtcatcaag ggctttattt aaaagttgtt catagtcctt
     1861 tataagtgag attttatcgc tgtaaatacc tgcgtacttg agccttacag ccatatcctc
     1921 cgctctaatt cccgaggcga agaacaatga tatgttttcc tgaatatttt ccagtttttc
     1981 aaaatacaca tcccacagcc atgaaatatc tgtaccgtct gcaagcttat catttataat
     2041 gaaagcaatt tgagctttct gcgtttctgt aagaagatag gtaagtacct ggttaaaacc
     2101 tgtgggattt ttcacaagaa ttatttttat ggttttgccg ttagtcacaa gggtttccat
     2161 ccttccaaaa ccgcattcga aactttccat cgcctttata atactatctg caggaaaacc
     2221 taataaatga cctaatgcag ctgcagccaa tgcattgtat atgttgtaaa gaccgggaag
     2281 gtttattctg gctgtaaaag acggcttttt gctgtcatca cccgcatttt ccagtgagaa
     2341 tactatgtct gaatacgaac tgttaaggtc attaacccca aggcaagtaa cctgggggga
     2401 aggacggcta taaccgcaat ttgggcagga gaaagcccca agatgaccgt aaacctggta
     2461 tgaatactca tatctgtttt tgcagtaaag gcagtataca gcatccgagt tttcaacctt
     2521 ttcaccgctg tcggatgcac cctgggcaaa accgtaatat ataacatctc ttttaacgtt
     2581 gtgg
//
LOCUS       DAOOJC010000355         2567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_31467_length_2567_cov_3.542197, whole genome shotgun sequence.
ACCESSION   DAOOJC010000355 DAOOJC010000000
VERSION     DAOOJC010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31467_length_2567_cov_3.542197"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="PLA01_08865"
     CDS             <1..1512
                     /locus_tag="PLA01_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 48 protein"
                     /protein_id="HPD01442.1"
                     /translation="MEYYYLTGDKNISELCRKWASWAMENTYLHSDGSYEIPCTLEWS
                     GQPDTWTGKPSSNKNLHCTIKDWTVDAGVTAALAKALIYYAAATEKHEKALNEKARET
                     SKQLLDRMWIDYKDDLGISSPESRDDFKRFFDEVYVPVDFSGTNAQGAEIKNGTTFIE
                     MRPKYKSDPGFSAIEQAVNSGTAPLMKYHRYWVQAEIAMANAMYHIYFEHTGNGTVPG
                     IKQEVNKGDSPTPDNTPVVTPIETSDPTPTVTSTQTPEPTNTPTIMPTGTNNSTHTPT
                     IKPTPTHKATNTPTKRPTYTPTKKPTNTPTKKPTNTPTKKPTNTPTKRPTYTPTKRPT
                     NTPTKRPTYTPTKRPTNTPTKKPATPSNKVLLQYYNNNANEMTSNINVSIKLTNNSDS
                     SIRLSDVKIRYYYTIDGEKPQNFACDWSTVGSSNVTGRFAKLPSSSSSADYYLEIGFT
                     DGAGSLESNTSIDIQTRFWKSDWSNYILNNDYSTSSGKIALYVSGKLVSGSEP"
     gene            complement(1669..>2567)
                     /gene="asnS"
                     /locus_tag="PLA01_08870"
     CDS             complement(1669..>2567)
                     /gene="asnS"
                     /locus_tag="PLA01_08870"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPD01443.1"
                     /translation="DAEGAGQMFRVTTLDINNLPKTEDGKVDFSKDFFDRETNLTVSG
                     QLEGETYCMAFRNIYTFGPTFRAENSNTARHAAEFWMIEPEIAFADINDDMELAEDML
                     KYIINYCMENAPEEMEFFNNFVDKTLFDRLSNIVNSEFAHVTYTEAIDILSKAAVKFD
                     YPVKWGVDLQTEHERYLTEQVFNKPVFVTDYPKEIKAFYMRMNDDNKTVAAMDLLVPG
                     VGEIIGGSQREERLDLLEKRMEELNLKKEDYWWYLDLRRYGSCKHAGFGLGFERAIMY
                     ITGMTNIRDVIAFPRTVHNAEF"
BASE COUNT          822 a          630 c          438 g          677 t
ORIGIN      
        1 atggaatatt actatttgac cggcgataaa aatataagtg agctttgcag gaaatgggca
       61 tcgtgggcaa tggaaaacac ctatctgcac agtgacggtt cgtatgaaat tccttgcact
      121 ttagaatgga gcggtcaacc tgatacatgg acaggcaagc cttcttcaaa taagaacctt
      181 cattgcacaa taaaagactg gacggtcgat gcaggtgtta ccgctgcctt agcaaaggca
      241 ctcatatact atgctgctgc taccgaaaag catgagaaag cacttaatga aaaagccaga
      301 gaaacttcaa aacagcttct tgatagaatg tggatagatt acaaggacga tttgggaatt
      361 tcctctcctg aatcaagaga tgatttcaag cgattttttg atgaagtgta tgttcctgtt
      421 gacttctccg gtaccaatgc tcaaggagca gaaataaaga acggaactac ctttattgaa
      481 atgcgtccca aatacaaaag tgaccccggc ttctctgcta tagaacaagc ggttaacagc
      541 ggaactgctc ctttaatgaa atatcaccgt tattgggttc aagccgaaat tgcgatggca
      601 aacgcaatgt atcacattta tttcgaacat acgggaaacg gaacagtacc gggtattaaa
      661 caagaagtaa ataaaggtga ttctcctact ccggataata cacctgttgt tacccctatt
      721 gaaacttcag atccgacacc tactgttaca tctactcaaa ccccagaacc taccaataca
      781 cctacaataa tgcctaccgg cactaataat tcaacgcata cacctacaat taaacctact
      841 ccaacccata aagcaactaa tacaccaact aaaagaccga cctatacgcc tactaaaaag
      901 cccactaaca ctccaaccaa gaaaccaacc aatacaccta ctaaaaaacc cactaacact
      961 ccaaccaaga gaccgaccta tacacccact aaaagaccca ctaacactcc aaccaagaga
     1021 ccgacctata cgcctactaa aagacccact aacactccga ctaaaaaacc ggctactcca
     1081 tcaaacaaag tattgctgca gtattataac aacaatgcca atgaaatgac cagcaacatt
     1141 aatgtaagta ttaaacttac gaataattcg gattcaagca ttcggctttc ggatgtaaag
     1201 ataaggtatt actatactat tgacggtgaa aaacctcaga atttcgcttg tgactggagt
     1261 accgtcggca gttccaatgt caccggccgg tttgcaaagc ttccttcttc gagcagcagt
     1321 gccgattact acttggaaat aggcttcaca gacggagccg gcagtttaga gtccaacacc
     1381 agcatagaca ttcagacaag attctggaag tcggattggt ccaactacat actgaacaat
     1441 gattactcca caagctccgg caaaattgcc ctctatgttt caggaaagct ggtttccgga
     1501 agtgagccat agatattaag agaaaaagag ccaaaaggta caatcctgac ttcctttcgg
     1561 ctctttatca atttatccgc tacttatcct gaaaaatcca tcttccataa agcagattta
     1621 ctaatcctat ttcttcataa aatcctttcg accagttaat ctgtagtatt aaaactctgc
     1681 attatggaca gttcttggga aagctatcac gtctctaata tttgtcatac ctgtaatata
     1741 cataatagcc ctttcaaaac cgagaccaaa tccggcatgc ttacagcttc cgtatctgcg
     1801 aagatccaga taccaccagt agtcctcctt tttaaggttg agctcctcca tcctcttttc
     1861 aagaaggtcc agtctttctt ccctctggct tcccccaatt atttcaccca caccgggaac
     1921 aagaaggtcc atggctgcaa cagtcttgtt gtcgtcattc atcctcatgt aaaaagcttt
     1981 aatttccttt ggataatcgg taacaaatac aggcttatta aacacctgct cagtcagata
     2041 cctttcatgc tctgtctgaa ggtcaacacc ccacttaacc ggataatcaa actttaccgc
     2101 agctttgctg agaatatcaa tggcttcggt gtatgtaaca tgggcgaact cagaatttac
     2161 tatgttgctc aatctgtcaa aaagcgtttt gtcaacaaaa ttattaaaga attccatttc
     2221 ttccggagca ttttccatac agtaatttat aatatacttt agcatgtcct ctgcgagctc
     2281 catatcatcg ttaatatctg caaatgctat ctccggttct atcatccaga attcagccgc
     2341 atgccttgca gtgttggaat tctctgccct gaaggtaggc ccaaaggtgt atatattcct
     2401 aaaggccata caataagtct caccctctaa ctgaccgctg acagtaagat ttgtctccct
     2461 gtcaaaaaag tctttggaaa aatcaacttt tccatcctca gtcttaggca aattattaat
     2521 atcaagagtg gttaccctga acatctggcc ggcaccttcc gcatcgc
//
LOCUS       DAOOJC010000356         2562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_31561_length_2562_cov_3.615876, whole genome shotgun sequence.
ACCESSION   DAOOJC010000356 DAOOJC010000000
VERSION     DAOOJC010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31561_length_2562_cov_3.615876"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /gene="dxr"
                     /locus_tag="PLA01_08875"
     CDS             <1..671
                     /gene="dxr"
                     /locus_tag="PLA01_08875"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPD01444.1"
                     /translation="NKEDVSKIILTASGGPFRGKRMDELRNVSVKEALKHPNWSMGSK
                     ITIDSATMMNKGLEVIEARWLFDVPLDRIEVLIHPQSIIHSMVEYKDGAIMAQLGSPD
                     MRLPIQLALTYPRRLMNSFSKLDLLKNNSLTFEAPDTEAFPCLKLAYEALRTGGTMPA
                     VMNAANEEAVRLFLEERIGFTEIPQIIQRVMESHSVKSVPDLDDIIGMDLWARGRVKE
                     LICN"
     gene            693..1973
                     /gene="rseP"
                     /locus_tag="PLA01_08880"
     CDS             693..1973
                     /gene="rseP"
                     /locus_tag="PLA01_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPD01445.1"
                     /translation="MRLLMVFMAFNLIIIVHELGHFIVAKLSGIKVDEFSLFVGPKIF
                     SFKRGETTYSLRVFPILAYVKMEGEDEESDSERAFSNKPVWVRASVIAAGPLSNLLLA
                     FIIICIVYTATGYSTRTIDEVQVGSAAYNAGIMEGDTIIGYNGKHIYDPMEVVQFLYI
                     SKGEPTKIVVKRDGVKEPIEKDIVPTIEREYLLGFYADPAPGKSNVVADLAMGGPAEK
                     SGMQVGDRITALNGAEVTSIEEIKAFLKENKEAPVDVTVDRQGGKQVLKVTPRLTENY
                     YLGIEFSKVEKGSLLGIMSSAAGFTYSNIRMVPYSLYWLATGQVSITEMTGPVGIVST
                     MNDVAQKSESFMEALLQILLWTGLISAAIGATNLVPFPALDGSKLLILAIEAISRKKI
                     PVEKEAIITSIGFFILIGLSIFVMANDIMRFIVK"
     gene            2116..>2562
                     /locus_tag="PLA01_08885"
     CDS             2116..>2562
                     /locus_tag="PLA01_08885"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPD01446.1"
                     /translation="MDYIERKKTKKIRVGDVSIGGGSPVAVQSMTNTDTRDIKATVNQ
                     IKMLEEEGCRIVRLAVPDNEAAEAIGHIKKSVKIPLVADIHFDYRLAITSMEMGADKI
                     RINPGNIGDKERVKKVVEIAKQRNVPIRIGVNAGSLEKNITQKYGLT"
BASE COUNT          778 a          426 c          668 g          690 t
ORIGIN      
        1 ataataaaga ggatgtatcg aagattatat taactgcttc tggcggaccc ttcagaggaa
       61 agaggatgga tgagcttaga aatgtatcgg taaaggaagc actgaaacat cccaattgga
      121 gtatgggaag caaaataacc attgattctg caacaatgat gaacaagggt ctggaagtaa
      181 ttgaagcccg ttggcttttt gatgttccgt tggacaggat tgaggttttg attcatcccc
      241 agagtatcat tcactcaatg gtggaatata aggatggggc gattatggct cagttggggt
      301 ctcccgacat gagacttccg atacagcttg cacttacata ccccagaagg cttatgaaca
      361 gcttttcaaa gcttgacctt ttgaagaaca acagccttac ttttgaggct cctgacacag
      421 aagcttttcc atgccttaag ctggcatatg aggcattaag gactggtggg acaatgcccg
      481 cagttatgaa tgctgcaaat gaagaggcag taagattgtt tttggaagaa agaattggtt
      541 ttacagaaat accgcagata atacaaaggg ttatggaaag tcactcggtc aaaagtgtac
      601 cggatcttga tgatataatc ggtatggatt tatgggcaag aggcagggtt aaggaattga
      661 tctgcaatta atgcgtttga aggaggtttt caatgagact tttgatggtt tttatggcat
      721 tcaatttaat tattatagta catgagttgg gacactttat tgttgcaaag ctgtcgggta
      781 taaaggttga tgagttttcc ctttttgtag ggcctaagat tttcagtttc aaaagagggg
      841 aaacaacgta ttctttaagg gtgtttccca tccttgccta tgttaagatg gaaggtgaag
      901 atgaggaatc cgacagtgaa agggcattta gcaacaaacc tgtgtgggtg cgggcttcgg
      961 ttatagcagc gggacctctt tccaatttgc ttttggcgtt cattattatc tgcattgtat
     1021 acactgccac gggttatagc accaggacca tagacgaggt gcaggtaggc tctgcagctt
     1081 ataatgcggg tattatggag ggagacacca ttatcggtta taacggcaag cacatatacg
     1141 atccgatgga agtggtacag tttttgtata tttcaaaggg agaaccgaca aaaattgtag
     1201 ttaaaagaga cggagtaaaa gaacccattg agaaagatat tgtgcccaca atcgagaggg
     1261 aatacctgtt gggtttttat gccgatcctg caccgggaaa atccaatgtt gtagctgatc
     1321 tggctatggg aggaccggcg gaaaagtcgg gtatgcaggt gggtgacaga ataacagcct
     1381 taaacggtgc agaggttacg agtatagagg agataaaggc ttttttaaag gaaaacaagg
     1441 aagcaccggt ggatgttacc gtagacaggc agggtggcaa gcaggtgctt aaggttacac
     1501 cccggttaac tgaaaactat taccttggaa tagaattctc caaggtggag aagggcagcc
     1561 ttttaggtat tatgagcagt gctgccggat ttacctattc caatatccgc atggtacctt
     1621 acagcctgta ctggcttgcg accggtcagg tatccataac ggaaatgacc gggcctgtgg
     1681 gtattgtcag cactatgaat gacgttgctc aaaagagtga aagctttatg gaggcacttt
     1741 tgcagattct tctctggacg gggcttatca gtgcggcaat cggtgccacc aaccttgtgc
     1801 cctttccggc cctggacgga agcaagcttt tgattttggc tatcgaggct ataagcagga
     1861 agaagattcc cgtagaaaag gaagcaataa ttacatcaat aggattcttt attttgattg
     1921 ggctttccat attcgttatg gccaacgata ttatgagatt tattgtaaaa taacattact
     1981 aatgctgtat tttatagctt tgcataggat gtaaggtaat tttcaactaa ggtaaaattc
     2041 tctttgaatt gaagtgatat aaacccgatg agtttggacg gtatttggaa ttattttatg
     2101 agggagaagt atttgatgga ttacattgaa aggaaaaaaa cgaagaaaat aagagtcggt
     2161 gatgttagca tagggggcgg atcgcctgtt gccgttcagt caatgaccaa taccgacaca
     2221 agggatataa aggctactgt aaaccagata aagatgttgg aggaagaagg atgccggata
     2281 gtaaggctgg cagtaccgga caatgaagcg gcagaggcca taggacacat aaaaaaatct
     2341 gttaaaatac ctctggtggc tgatatacac tttgattacc ggcttgccat aacaagcatg
     2401 gaaatgggtg ccgacaagat aaggattaat cccggtaaca tcggagacaa agaaagggtt
     2461 aaaaaggttg ttgagattgc aaagcaaagg aatgtaccca ttcgtatcgg agtgaatgcc
     2521 gggtctttgg agaaaaatat tacacaaaag tacggattga ct
//
LOCUS       DAOOJC010000357         2548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_31822_length_2548_cov_3.401524, whole genome shotgun sequence.
ACCESSION   DAOOJC010000357 DAOOJC010000000
VERSION     DAOOJC010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31822_length_2548_cov_3.401524"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /gene="rpmA"
                     /locus_tag="PLA01_08890"
     CDS             <1..231
                     /gene="rpmA"
                     /locus_tag="PLA01_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPD01447.1"
                     /translation="STRNGRDSESKRLGVKRGDGQFVKAGNILVRQRGTRIHPGINVG
                     KGSDDTLFAMADGKVKFSRLGKDRKQVSIITA"
     gene            330..1607
                     /gene="obgE"
                     /locus_tag="PLA01_08895"
     CDS             330..1607
                     /gene="obgE"
                     /locus_tag="PLA01_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPD01448.1"
                     /translation="MFIDNARIYIKAGNGGNGAVSFHREKYINKGGPDGGDGGKGGDV
                     VLIVDEGLRTLQDFRYKRKYIAENGENGKGSNCTGKSGENLIVKVPPGTIVKDEQTGR
                     VLADLIHAGQQVVIAKGGKGGAGNRHFATSTRQIPNFAKAGDEGEEFWAILELKLLAD
                     VGLIGFPNVGKSTILSMVTAAEPKIANYHFTTIDPNLGVVNIDAENGFVMADIPGIIE
                     GAHEGIGLGHKFLKHVERTKLLIHVVDISGSEGRDPVKDFDTINEELKKFNPALYERP
                     QVIAANKMDVTGAKELLAVFKEVYEPKGYKILPVSAAANSGLKELIYYVAQRLKELPD
                     TVLSDFEEKEVAVYNVKDDDSYKIYKENQVYVVEGSWIKKLVASTNFNDHESLQYFQR
                     TIKRKGIVEELENMGIQEGDTVKMYDLEFEYFR"
     gene            1772..1960
                     /locus_tag="PLA01_08900"
     CDS             1772..1960
                     /locus_tag="PLA01_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01449.1"
                     /translation="MECLNCGNCKIGSTAYYCLMKNDFVLNEEATSRVVEKSRSGWKK
                     GHPGYEVNRRKSRKNVEV"
     gene            2143..2433
                     /gene="yhbY"
                     /locus_tag="PLA01_08905"
     CDS             2143..2433
                     /gene="yhbY"
                     /locus_tag="PLA01_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HPD01450.1"
                     /translation="MLTGKQRSYLRSLANGIDPVFQIGKGGVNDNMIKQFNEALEARE
                     LIKVTVLKNSLDDVREVCDEAARLTGAEVVQVIGNKFVLYKESKENKVIELP"
     gene            complement(2453..>2548)
                     /locus_tag="PLA01_08910"
     CDS             complement(2453..>2548)
                     /locus_tag="PLA01_08910"
                     /inference="COORDINATES: protein motif:HMM:NF025635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HPD01451.1"
                     /translation="KNDARRICENWKKNSQTIYSEIIESLLKDRN"
BASE COUNT          860 a          353 c          639 g          696 t
ORIGIN      
        1 agtacaagga acggtcgtga cagtgagtct aaaaggcttg gagtaaaaag aggagacggt
       61 cagttcgtaa aggctgggaa tattctggtt agacagagag ggacaagaat ccatcccggt
      121 ataaacgtcg gtaaaggcag tgatgataca ttgtttgcca tggcggacgg aaaagtgaag
      181 ttctcaagat taggcaaaga caggaaacag gtaagtatta ttacagcata gagaatattt
      241 aaaaatcccg gtttaaccgg gatttaattt ttatatatga gatatactaa gtactataaa
      301 aatagtatgt tccatggagt tgagcggtta tgtttattga taatgcaaga atatatataa
      361 aagctggaaa tggtggaaac ggtgcagtat cctttcatag ggaaaagtac ataaacaagg
      421 gtggtcctga cggcggcgac ggcggtaagg gcggcgatgt tgttttaatt gtggatgaag
      481 ggcttaggac attgcaggac ttcaggtata aaagaaaata cattgccgaa aacggtgaaa
      541 acgggaaggg ttccaattgc accggaaaaa gcggggaaaa cctgatagtc aaggttcccc
      601 cgggaactat agtaaaggat gaacaaacgg gcagggttct tgcagacctt atacatgccg
      661 ggcagcaggt tgtgattgct aaaggcggta agggcggtgc cggcaaccgt cattttgcca
      721 catccacaag acagataccg aattttgcaa aggcaggaga cgagggggaa gagttttggg
      781 ccattttgga gctaaagctt ttggctgacg ttggactcat tggctttccc aatgtaggta
      841 aatccacgat tttgtcaatg gttactgccg ctgaacccaa aattgcaaat tatcatttta
      901 caacaattga cccgaattta ggcgttgtaa atatcgatgc ggagaacggc tttgtaatgg
      961 cagatatacc gggaattatc gagggtgccc atgaaggcat aggtcttggt cataagtttt
     1021 taaaacatgt ggagagaacg aagcttctga ttcatgttgt tgatatttca ggctcggaag
     1081 gcagagatcc tgtaaaagat tttgatacaa ttaatgaaga gttgaagaaa tttaaccctg
     1141 cattgtatga aaggccacag gttattgctg caaataaaat ggatgttaca ggtgccaaag
     1201 agctgctggc agtgttcaaa gaagtatatg agcctaaggg gtacaagatt ttaccagtat
     1261 ctgcagcagc gaatagcggt cttaaggagc ttatttatta tgtagcacaa aggcttaaag
     1321 aacttcctga tactgttttg tcagattttg aggaaaaaga agtggcagta tataatgtta
     1381 aggacgacga ttcttataag atttataagg agaatcaggt ttatgtagtt gaaggaagct
     1441 ggataaaaaa gcttgttgca tctaccaatt ttaatgacca tgagtcttta cagtatttcc
     1501 agagaacaat aaaaagaaaa ggtatagtgg aagaacttga gaacatggga atacaagaag
     1561 gagatacagt caaaatgtac gatctggaat ttgaatattt ccggtagtat attcagctaa
     1621 aagaatttga caaatagtaa atttgtacta ttaatatagt attgcaagag gattattgca
     1681 aaacaatata gacaaaaacg cccaaagtgt tgtaaaatat ataatataat ataatacatg
     1741 cttgtaaaat ggaggggcat tagtaagttt tatggaatgt ttaaattgtg gaaattgtaa
     1801 gatcggtagt acagcgtatt attgtttaat gaagaacgac tttgtgctga acgaggaagc
     1861 cacatcccgg gttgttgaga agagcagatc ggggtggaag aagggacatc cgggttatga
     1921 ggtcaacagg aggaaaagtc gaaaaaatgt tgaggtgtga ggagtaagat tagggataga
     1981 gcattctatc cctattgttt aatgggattt tataaagaga aatgtggaag aaacttaagt
     2041 ttcccgaata tttttcttta ccgttgttaa gggtaattaa aggacatcca taatatcaat
     2101 tattgaatat aatgttaaaa gtagtggaaa aggagaaaaa ttatgcttac aggaaagcaa
     2161 cgcagttatt taagaagcct tgcaaatgga attgaccccg tatttcagat tggcaagggt
     2221 ggagtcaatg acaatatgat aaagcagttt aacgaggctt tggaggctag agaactaata
     2281 aaagtgacag ttcttaagaa ttcattggat gatgttaggg aagtatgcga tgaagcagcc
     2341 agactcacag gggctgaagt agtacaggtt ataggcaata aatttgtttt atacaaggaa
     2401 tcaaaggaaa ataaggttat agaattacct tagggggcat gctgcccccg ttttagtttc
     2461 tgtcttttaa aagactttca attatttcac tgtaaattgt ctgagaattc tttttccagt
     2521 tttcacagat gcggcgtgca tcattctt
//
LOCUS       DAOOJC010000358         2547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_31841_length_2547_cov_3.128411, whole genome shotgun sequence.
ACCESSION   DAOOJC010000358 DAOOJC010000000
VERSION     DAOOJC010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31841_length_2547_cov_3.128411"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLA01_08915"
     CDS             complement(<1..501)
                     /locus_tag="PLA01_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD01452.1"
                     /translation="MKLVVLGNNGPYPSAGGACSGYLVVEGDTKILIDCGNGILSNLQ
                     KFIKHEDLDAVILTHLHSDHMSDMMVLRYAIQIKMNRGQDIRPLDVYVPSEPLEEYRR
                     LDNKGVFNLNPISEDTVLNFGNLKITFKDMTHPVKCFAVAIDNTEKRFVFSGDTSWNN
                     NIIDFSR"
     gene            complement(656..1993)
                     /locus_tag="PLA01_08920"
     CDS             complement(656..1993)
                     /locus_tag="PLA01_08920"
                     /inference="COORDINATES: protein motif:HMM:NF025644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HPD01453.1"
                     /translation="MKRLQLDAIAFLFIIGVLGIANFINVNKPKISEMENRALKQKPE
                     FKVSALLDGSYFSEYTEYYSDTFIMRDRLIKISNGIQQALFMKDSDVKIIVSDKSKEY
                     NKEPEPIAEENDGEISSPPDGTMPANTPKPSFTPTPTKKPYNESDGVGYWIVIDGKAV
                     ELFKFNKDSFDFYARVLNEYNQKIDGKMPIYSLIAPTNSEFVQLRNYKGITDSQNNAI
                     EYLNSKFNEGITSVNAYDVLNLHKDEYIYFRSDHHWTALGAYYAYTAFMEAKGEEPVP
                     LDKYETVQIDNFLGSTYAKTMDKSIEKNPDTIYAYLPFVDYKYEKHRFYECSEADIID
                     MKYADTKLDKYLVFLSAGDATWAKISTENKNGKKLLVIKDSYGNSFVPFLLPHYEEIY
                     VIDPRFYDFNTSEKNVADFVESKGINEILFINYMEDVNYKEYMQSLEELINHQ"
     gene            complement(2120..>2547)
                     /locus_tag="PLA01_08925"
     CDS             complement(2120..>2547)
                     /locus_tag="PLA01_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HPD01454.1"
                     /translation="VLLIIEKFVMSKIEKKIPNIIWRLYFVVAVLVGWVFFYHTSIDK
                     AFQFLGIMFGINASSWSSPEVGIFLNSYAIFISIAIIGCTPLVKTVFGRIKGRLQKKQ
                     IGIYTADQLIKSTVNIAILIVSIIMLVGQSYNPFLYFKF"
BASE COUNT          746 a          537 c          410 g          854 t
ORIGIN      
        1 tctggaaaag tcaattatat tgttgttcca tgatgtatcc cctgaaaaaa caaacctctt
       61 ctctgtgttg tcaattgcta cggcaaaaca cttaacaggg tgcgtcatat ctttaaaagt
      121 aatcttcaag tttccaaaat tcaagacggt atcttcagaa ataggattta agttaaaaac
      181 acctttattg tcaagccttc tatattcctc caaaggctct gaaggtacat atacatcaag
      241 gggtctgata tcctgtcccc tgttcatctt tatctggatt gcgtatctta aaaccatcat
      301 atccgacatg tggtcactgt gcaaatgagt cagtattacc gcatccaaat cctcatgctt
      361 tataaatttc tgaaggttgc tcaatatacc gtttccacag tctattagaa tttttgtatc
      421 accttcaacg acaagatacc cggaacacgc accccctgcc gatggatacg gaccgttatt
      481 tcccaataca acaagtttca ttaatgccac actcctcccg atttatgtta ataaatatta
      541 tatttcagtt tatcaaataa actcgataaa cccaacaaaa cttatatttt ctgcggcatt
      601 ttactttgtc attattaatg cacccacata attttcctta taagttcaat ataaactact
      661 gatgattaat cagttcttca aggctttgca tatattcttt gtaattgaca tcctccatat
      721 aatttataaa aaggatctca tttattcctt ttgattcaac aaaatcagca acattctttt
      781 cagaagtatt gaaatcataa aaccttgggt ctattacata tatttcttca tagtggggca
      841 gaaggaaggg tacgaagctg ttgccatagg aatcttttat taccaaaagc tttttcccgt
      901 ttttattttc tgtacttatt ttggcccagg ttgcatcccc ggcacttaaa aacaccagat
      961 acttatccag ttttgtatcc gcatacttca tatcaattat atcagcttca ctgcattcat
     1021 aaaagcggtg cttttcgtat ttgtaatcta caaacggcaa ataggcatat atggtgtcgg
     1081 gattcttttc aatactttta tccatagttt tggcatatgt actgcctaaa aaattatcaa
     1141 tttgcactgt ttcgtattta tcaaggggta ccggctcctc tccctttgct tccataaagg
     1201 cggtataagc ataatatgcc cccaaagctg tccagtggtg atcgcttctg aaatagatat
     1261 attcatcctt gtgaaggttt aatacatcat acgcattaac tgaggtgatt ccctcgttga
     1321 attttgagtt aaggtactct atcgcattat tctgggaatc ggttattccc ttgtaattcc
     1381 taagctgaac aaactcactg tttgtaggtg caatcaatga ataaataggc attttaccgt
     1441 caattttctg attgtattca ttaagaaccc gggcataaaa atcaaagctg tccttgttaa
     1501 acttgaagag ctctacggct ttaccgtcga tgacaatcca gtatccgaca ccgtcgctct
     1561 cgttataggg cttttttgta ggcgttggtg taaatgaagg tttgggagta tttgcaggca
     1621 ttgtcccatc cggtgggctc gaaatctcac catcattttc ttcagcaatt ggttcaggct
     1681 ctttgttata ctctttagac ttgtccgaaa caattatttt aacatcggaa tctttcataa
     1741 ataacgcctg ctgaataccg ttactgattt ttataagcct gtccctcata ataaaagtgt
     1801 cactgtagta ttcagtgtat tcactaaaat agctgccgtc caatagtgca gatactttaa
     1861 actccggctt ttgctttaat gccctgtttt ccatttcgga tatcttaggc ttgtttacat
     1921 taataaaatt agcaatgcct aaaactccaa ttataaataa aaatgctatt gcatccaact
     1981 gcaacctttt cattgcaaaa cgcctccatt atacatgtta gttgtggggt gtactgacaa
     2041 aatctccggg ggcatcgaac cccttcgctt tttggtcatt tcacttcgtg aaatcccatt
     2101 aataagtggc ttcgcatctc taaaacttaa agtagagaaa cgggttgtat gattgaccga
     2161 caagcataat aatagacact ataagtatcg ctatgtttac cgttgatttt atcagctggt
     2221 cggcagtata aatcccgatt tgtttcttct gcaatctccc ttttattcgc ccgaaaactg
     2281 ttttgacgag cggtgtacac cctataattg ctatcgaaat aaatatcgca tagctgttaa
     2341 ggaaaattcc tacttcggga cttgaccagg atgaagcatt aatcccaaac ataattccta
     2401 aaaactgaaa cgccttgtca atactggtgt gataaaagaa tacccagccc accagtactg
     2461 ccactacaaa ataaagtctc cagattatgt tgggtatttt cttttcaatt tttgacatga
     2521 caaacttttc aattataagc agcacaa
//
LOCUS       DAOOJC010000359         2534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_32082_length_2534_cov_3.649455, whole genome shotgun sequence.
ACCESSION   DAOOJC010000359 DAOOJC010000000
VERSION     DAOOJC010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32082_length_2534_cov_3.649455"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..>2534
                     /gene="mutS"
                     /locus_tag="PLA01_08930"
     CDS             35..>2534
                     /gene="mutS"
                     /locus_tag="PLA01_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPD01455.1"
                     /translation="MANLTPMMQQYLEIKEQYNDCILFFRLGDFYEMFFSDAETASRE
                     LEITLTGRDCGLEERAPMCGVPFHSSDSYISKLINKGYKVAICEQVEDPALAKGLVKR
                     DVIRVVTPGTVTDSSMLDEKKNNYLMSVYKNKSFYGIALVDLSTGDFLSTGITFGNTF
                     NKLMDEIAKFSPSEIVVNGEFFHDEAICSAVKKRFNVYFSSMEDSFFEREFSLNKVKS
                     YFKDFEAKENTFDLYINASGALLEYLEQTQKMSLSHIQNFNIYKIEEYMVLDLSTRRN
                     LELTETMREKSRKGSLLWVLDRTVTSMGGRTLRKWIEQPLINLHDIRDRLNAVGEFKD
                     KFMVRMEIRELLKAVYDIERLMGKVILGSANGRDLVAIKSSIGQVPYIKEMVKDFEAS
                     LNVISHNELDTLTDIYELIDKAIIDEPPVSLKDGGIIKTGFNEEIDRLKSASKDGKKW
                     IVDLESKERERTGIKNLKVGFNKVFGYYIEVTKSYYSQVPDHYIRKQTLANCERYITQ
                     ELKEIEDTVLGAEDKLVELEYQVFADVRNRVANEIDRLKRTAGSLSRIDALCSLAEVA
                     DRESYVMPDVTEEDEIEIVDGRHPVVEKMTGQEVFVPNDTRLNMGEDQIAIITGPNMA
                     GKSTYMRQVALIVLMAQIGSFVPAKSARIGIADRIFTRVGASDDLAAGQSTFMVEMSE
                     VANILSNATKKSLLILDEIGRGTSTYDGLSIAWAVIEYISEKIGARTLFATHYHELTE
                     LEEKMDGVKNYCISVEEKGEDIVFLRKILRGGADNSYGIQVAKLAGVPEKVIGRAKEL
                     LKELKDADITKRENRAKRGGKPIEGQIDVFSFNVE"
BASE COUNT          774 a          438 c          683 g          639 t
ORIGIN      
        1 cgagtaatat taatttgtac gtgaggtaag taaaatggct aatttgacac cgatgatgca
       61 acagtatctt gaaataaagg aacagtacaa tgactgtatc ctttttttca ggctgggaga
      121 cttttatgaa atgtttttct ctgatgccga gacggcatca agagagcttg agataacgct
      181 tacggggaga gattgcggtc ttgaggaaag ggcacctatg tgtggagttc cctttcattc
      241 ttcggattct tacatatcta agcttataaa taaaggttac aaggtggcga tctgtgagca
      301 ggtggaggat ccggcactgg caaaagggct ggttaagagg gatgtcataa gggtggttac
      361 gcccggtacg gtaacggatt cttccatgct tgatgaaaag aagaacaact accttatgtc
      421 ggtgtacaaa aacaagagct tttacggtat tgcccttgtg gacctttcaa cgggagattt
      481 tttatccacc ggtattacct tcgggaacac ctttaataag ttaatggatg aaattgcaaa
      541 attctcaccc tcggaaattg tggtaaacgg tgagtttttt catgatgaag caatttgttc
      601 ggccgttaaa aaaagattta acgtgtattt ttcaagcatg gaggatagtt tttttgagag
      661 ggaattctcc ctcaataaag tgaagagtta tttcaaggat tttgaagcaa aggaaaacac
      721 ctttgacctt tacataaacg catcgggtgc acttttggag tatcttgaac agacacagaa
      781 gatgagcctt agccatattc aaaacttcaa catatacaaa attgaggagt atatggtttt
      841 agacctgtct accagaagga accttgagct taccgaaacc atgagagaaa agagcaggaa
      901 gggttctttg ctttgggttt tggacaggac ggtgacatcc atgggcggcc gcaccttgag
      961 aaaatggatc gagcagcccc ttattaacct tcacgacata agggacaggc tcaatgccgt
     1021 cggtgagttt aaggataagt ttatggtgcg gatggagata agggagcttt tgaaggctgt
     1081 ctatgatatt gaaaggctga tgggtaaggt aatcctcggt tctgccaacg gccgggacct
     1141 tgtggcaatt aaaagctcca taggacaggt tccttatata aaggagatgg taaaagactt
     1201 tgaggcaagc ctcaatgtaa tcagccataa cgagctcgat acccttaccg atatttatga
     1261 gcttattgac aaggccatca ttgacgaacc cccggtttcg ttaaaagatg ggggaattat
     1321 taaaacgggc ttcaatgaag agattgacag gttaaaaagt gcatcgaaag acggtaaaaa
     1381 gtggattgtg gaccttgaga gcaaggagag ggagcgaacc ggaataaaga atttgaaggt
     1441 gggattcaac aaggttttcg gctattatat cgaagtcacg aaatcctact actcccaggt
     1501 tccggatcat tatataagaa aacagaccct ggccaactgt gaaaggtata ttactcagga
     1561 attaaaagaa atagaggata ctgttttggg tgcggaagac aagcttgttg agttggagta
     1621 tcaggtattt gcagatgtta gaaacagggt agcaaatgaa atagacaggc tgaaacgcac
     1681 agcagggagc ctttcgcgga tagatgcttt gtgctcttta gcagaggtgg cggacagaga
     1741 gtcctatgtt atgcctgatg tgacggaaga agatgaaata gaaattgttg acggcaggca
     1801 cccggtggtg gagaaaatga cggggcagga ggtttttgtc cccaatgata cccgtcttaa
     1861 catgggtgag gaccagatag ctattattac cgggcccaac atggccggta aatccacata
     1921 catgaggcag gtggcattga ttgtgcttat ggcacagata ggaagctttg tccctgccaa
     1981 aagtgcacgt atcggcattg cggacaggat attcaccagg gttggtgcat cggatgacct
     2041 ggcggcaggg cagagtactt ttatggtgga aatgtccgag gtagcaaaca tattgagcaa
     2101 tgccactaaa aaaagcctct tgattcttga tgaaatcgga aggggtacaa gcacttacga
     2161 tggcttgagc attgcctggg cggttattga gtatatatcc gagaaaatcg gtgcaagaac
     2221 cctttttgcc acacactacc acgaattgac cgagctggag gagaaaatgg atggcgtaaa
     2281 gaactattgc atatcggtgg aggaaaaggg agaagatatt gttttcttga gaaagatatt
     2341 aagaggcgga gcagataaca gctacgggat tcaggtggca aagctcgccg gtgtgccgga
     2401 aaaggttatc ggaagggcaa aggagctttt aaaggagcta aaggatgcag atattacaaa
     2461 gagggaaaac cgggccaaac gaggcggaaa gcctatagaa ggccagattg acgtattctc
     2521 ctttaatgtg gaac
//
LOCUS       DAOOJC010000360         2527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_32207_length_2527_cov_2.889968, whole genome shotgun sequence.
ACCESSION   DAOOJC010000360 DAOOJC010000000
VERSION     DAOOJC010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32207_length_2527_cov_2.889968"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PLA01_08935"
     CDS             complement(<1..919)
                     /locus_tag="PLA01_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPD01456.1"
                     /translation="MKLNEIVELDKKYFMNTFGNRTPVCFDHGKGINLWDLDGKKYYD
                     FLAGIAVSALGHSHPKLVNAIKEQAEKLIHCSNLYYIENQAKLAKILVENSCASKVFF
                     ANSGAEANEGAIKLARIFFKKKGMPEKFEIITLEKSFHGRTLATIAATGQDKYQKPYS
                     PLTPSFLKVPINDLGALEKAINGSTCAVMIEPIQGESGVNLTSIEYMQGVRKLCDEKG
                     ILLIFDEVQCGLGRTGKLFAYEHLGIEPDIFTLAKALGGGFPIGALCAKEHVASAFEP
                     GDHGSTFGGNPLACAAGLAVMEVMINDNLV"
     gene            complement(1282..1944)
                     /locus_tag="PLA01_08940"
     CDS             complement(1282..1944)
                     /locus_tag="PLA01_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPD01457.1"
                     /translation="MRKDLMSKVLKEDMVVNTRLSSSNIWIPNVVYKVENHMVTIGLL
                     SEYLENIIMMGQNMTLKVSNPKTEFLFEGEIVEISPDFPSYVTLKIRDIKKLKNIRTF
                     PRYDVYLGAVVKPVDSSEDSFAIVHNLSLVGMAFYSREDYKIGDQGYEFSIYLSDCKT
                     VKAIGKITRVSIKDHFSDYGFQFTEMNEENNILISNIFNSIEDEKDKIREEFLSCVKK
                     HL"
     gene            complement(2108..>2527)
                     /gene="argB"
                     /locus_tag="PLA01_08945"
     CDS             complement(2108..>2527)
                     /gene="argB"
                     /locus_tag="PLA01_08945"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPD01458.1"
                     /translation="LLAKDEYIPVVAPIGVGKDGQSYNINADTVAGEVAASLKAEKLM
                     LLTDVPGVKRHKDSNEIIPALTSPEVVDLIDKKIIDGGMIPKVLGCIDALEKGVGRTH
                     IIDGRIPHCLLLEIFTNKGIGTMIMKEKVLYHSNEKL"
BASE COUNT          750 a          517 c          445 g          815 t
ORIGIN      
        1 taaccagatt gtcatttatc ataacctcca taactgccag acctgcagca caggcaagag
       61 gatttccacc gaaggttgaa ccatggtccc caggctcaaa agcacttgct acatgttcct
      121 ttgcacacag ggctccgatg gggaagccgc cgcctaaagc ctttgcaagg gtgaatatgt
      181 caggctcaat acccagatgc tcgtatgcaa aaagcttacc ggtacgtccc agaccacatt
      241 gaacctcatc aaatatcagg agtattccct tttcatcaca aagcttcctg actccctgca
      301 tatactcaat agaggtaagg ttaacaccgc tttcaccctg tatcggttct atcattacag
      361 cacaggttga accgtttatt gccttttcaa gggctcccaa atcatttatg ggcaccttta
      421 aaaagcttgg ggtaaggggt gaataaggtt tttggtattt atcctgtccc gttgcagcaa
      481 tggtggcaag cgttcttcca tggaaggatt tttccagagt tattatttca aacttctcag
      541 gcataccttt ctttttaaag aagatgcgag caagttttat ggctccttca tttgcttctg
      601 ctccgctgtt tgcaaaaaac actttgctag cacaggagtt ttctacaaga atcttggcca
      661 gctttgcctg attttctata taataaaggt tggagcagtg aattaatttc tctgcctgtt
      721 cctttattgc attgacaagc ttgggatgtg agtgtcctaa agcacttacc gctatccccg
      781 caagaaaatc ataatacttc tttccgtcca gatcccagag gttgattcct ttgccgtggt
      841 caaaacatac aggagtcctg ttgccgaatg tattcatgaa atatttttta tccagttcga
      901 ctatttcatt tagtttcata agaaaaacct ccacgaaaaa taatatttat aattatacat
      961 aaaaatgtat aaaaatgcaa caactaaaat ttatacattg aaaactaacc gattaaattt
     1021 cttttcctca ataatccatg tgtgctaaga tccttgaaaa gggattcggt attatttata
     1081 cacaggaata tatatgattt cagaaaaaag aaaagggaag aagtgaaatc ctacaaataa
     1141 aaataaaccc taaggaatat aactcgggtt tatatattac tataagtgtg ataggaaaaa
     1201 tccagaagat tacaataaca gtacgactcc ggcgactgct gtgaaaagca agagctgagt
     1261 taatattatc tgaaggctta atcagagatg ctttttgaca cagcttaaaa actcttcccg
     1321 gattttatcc ttttcatctt caatagaatt gaatatgttt gaaataagta tattgttttc
     1381 ttcattcatt tcagtgaact gaaagccgta atcggaaaag tgatctttta tagacactct
     1441 ggtgattttt cctatagctt ttacagtttt gcaatcggaa agataaatag agaattcata
     1501 accttgatct ccgattttat aatcctctct tgagtagaat gccattccca ccaggctgag
     1561 gttatgtaca atcgcaaagg agtcttcact ggaatcaaca ggtttgacga ctgccccgag
     1621 gtagacatca taccttggaa aagtccttat attttttagc ttcttgatat cccttatttt
     1681 caatgtaaca taactgggaa agtcagggct gatttcgaca atttcacctt caaacaaaaa
     1741 ttctgttttg ggattggata ctttcagagt catgttttgt cccatcataa ttatgttttc
     1801 taggtactca cttaatagtc cgatggtaac catatggttt tcaactttat acactacgtt
     1861 cggaatccaa atattgctgg aagataatcg ggtattaaca accatgtcct ctttaaggac
     1921 ttttgacatc aagtctttcc gcaactgaat caatcctccc tgacaataat tactgtttat
     1981 tatctatcca tgtatagtgt aattttatac aatttactta tatttattct ttataaaatt
     2041 ctaaaatcct tcaaaaagtg attaaattca tcataattta tataatattc acatcaggaa
     2101 agccgaatca taatttttca ttgctgtggt agagtacttt ttctttcata atcatggtcc
     2161 cgataccttt gtttgtaaat atctcaagga ggagacagtg gggaatcctt ccgtcgataa
     2221 tatgggtcct tccgacgcct ttttcaaggg catcgataca accgagtacc ttagggatca
     2281 ttccgccgtc aattattttt ttgtcaataa gatcaacgac ttccggtgat gtcagggcag
     2341 gaattatctc attgctgtcc ttatgccttt taacacccgg aacgtcggta agaagcatga
     2401 gcttttctgc ttttaaagaa gcggctactt ctccggcgac cgtgtcagca tttatattgt
     2461 agctttggcc gtcctttccc acacctattg gtgccacgac aggaatatat tcatcctttg
     2521 ccaaaag
//
LOCUS       DAOOJC010000361         2524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_32259_length_2524_cov_2.969218, whole genome shotgun sequence.
ACCESSION   DAOOJC010000361 DAOOJC010000000
VERSION     DAOOJC010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32259_length_2524_cov_2.969218"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..902
                     /locus_tag="PLA01_08950"
     CDS             102..902
                     /locus_tag="PLA01_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HPD01459.1"
                     /translation="MAIEVFNRYEIKYLVDDAAFEKVQNILCNYMELDEYNKKTNSFY
                     TISNIYYDTFDNQLIRHSLSKPRYKEKLRLRAYGVPSLEDKVYLEIKKKVIGIVNKRR
                     SRLTLAEAYKFAETGRKPEYKAYMNEQVLNEIEYLFKKHILIPKLYLAYDRKAYFGIN
                     NRDLRITFDTNIRCRRTDLRLESGDYGERLLDDGIWLMEVKAEKSIPVWLSKMLSDNK
                     LFKTSFSKYGTEYKRTIMEKKMEGAEESCWMQYLMPQLTQHYPLEVQY"
     gene            839..1525
                     /locus_tag="PLA01_08955"
     CDS             839..1525
                     /locus_tag="PLA01_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HPD01460.1"
                     /translation="MLDAIFNATINTTLSFRSAILTIVVSFALGLLISYVYIKTHARA
                     NYSQNFTLTLIMIPCIIGVMILLIGSDVARAFSLAGAFSIIRFRSAPGDPKDISYVFF
                     AMAAGLACGVGLFAYAVFFTVCLCAFMVVLSKINFGARKTPYKVLKVVVPENLDYERE
                     FEDVFNKYTVSHELQKVKTTDLGTLYELVYSVNLYYGTNEKEFIDALRCRNGNLNIVL
                     GMKAESEYNS"
     gene            1647..>2524
                     /locus_tag="PLA01_08960"
     CDS             1647..>2524
                     /locus_tag="PLA01_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HPD01461.1"
                     /translation="MFCENKILIAKGNTPVYLLPQMANRHGLIAGATGTGKTVTLKVL
                     AESFSDSGVPVFLADIKGDLAGLAVEGADNPKIQERVEKLGIEGFEYRSYPVRFWDIF
                     GEGGHPVRTTISEMGAILLSRLLELNETQSGIMNIVFRIADDKGLLLIDLKDLRAMLQ
                     YVGENAKEFTIKYGSISTQSVGAILRKLIALEDQGGNYFFGEPDLNIFDFINVTSEGR
                     GYINILHSVKLVQNPILYTTFLLWLLSELFESLPEVDDVKIPKIVFFFDEAHLLFKDA
                     PKVLIQKIEQVVRLIRS"
BASE COUNT          797 a          382 c          572 g          773 t
ORIGIN      
        1 aaggaaaatt gataagtttc ctgcacaaaa gtagtgaatg ttgtttgact tgatatttcg
       61 tgcttttttt aaggtggggg gttgggaaag gagataaaga aatggccata gaggttttta
      121 atagatatga aataaagtat ctagtcgatg atgccgcctt tgagaaggtt caaaacatac
      181 tttgcaatta tatggaactg gatgagtata acaagaaaac taactctttt tacacaatca
      241 gcaatatata ctacgatact tttgacaatc aacttataag gcattcactg tcgaagccca
      301 gatataagga aaaattaagg ctgcgtgctt atggggtccc ttcgttggag gataaggttt
      361 atttggaaat aaagaaaaag gtaataggca ttgtgaataa aagaagatcc agactcactt
      421 tagcggaggc ttacaaattt gctgaaactg ggagaaagcc cgaatacaag gcttatatga
      481 atgaacaggt gctaaatgaa attgagtatt tatttaagaa acatattctt atacccaagc
      541 tttatctggc ttatgaccga aaagcatact tcggtatcaa caacagggat ttacgaatta
      601 ctttcgatac caatatcaga tgcagaagaa cagatttaag gcttgaatcg ggggattatg
      661 gtgagagatt actcgacgat ggtatctggc ttatggaggt taaggcagaa aagagtatac
      721 ccgtatggct ttctaaaatg ttaagcgata ataagctttt taagacgagt ttctcaaaat
      781 atggaaccga atacaagaga acaattatgg aaaagaaaat ggaaggggct gaagagagtt
      841 gttggatgca atatttaatg ccacaattaa cacaacacta tcctttagaa gtgcaatact
      901 gacaattgtt gtgtcgtttg cattaggatt gcttataagt tatgtatata taaaaacgca
      961 tgcaagggca aactattccc agaattttac tcttactttg attatgatac catgtattat
     1021 cggtgttatg attttattga taggaagtga tgttgcaagg gctttcagtc ttgcaggggc
     1081 tttttctatt atccggttcc ggagtgcacc aggggatcca aaagatatca gctacgtgtt
     1141 ttttgcaatg gcagctggtc ttgcttgcgg tgttggattg tttgcgtatg ctgtattttt
     1201 cactgtatgt ctctgtgcat ttatggttgt tttgagcaaa attaatttcg gtgcaagaaa
     1261 aacaccttat aaagtgctaa aagttgtagt tcccgaaaat ctggactatg agagggaatt
     1321 tgaagatgtt tttaataagt atacagtaag ccatgaactt cagaaggtta agactaccga
     1381 tcttggtacc ctgtatgagc ttgtgtattc agttaactta tactatggta caaatgaaaa
     1441 ggaattcata gatgctcttc gttgccgtaa cggaaatctt aatattgtac taggaatgaa
     1501 agcagaatcg gagtataata gttaataggt ggatatttgg tgcccctgag ttttgaaagc
     1561 agtatatcga ataagacgga tcaaaacaaa aggggcacaa tgcttcttta cctgcagtta
     1621 ataccatagt ataaggagag ataaatatgt tttgtgaaaa taagattctg atagcaaaag
     1681 ggaatacccc ggtttatctt ttacctcaaa tggcaaaccg ccatggatta attgccggag
     1741 caaccggtac aggaaaaacc gttactttga aggtattggc agagtccttt agtgatagtg
     1801 gtgttccggt ttttctagcc gatatcaaag gtgatcttgc gggccttgca gtagaaggag
     1861 ctgataaccc caaaattcag gaaagagtcg aaaagcttgg cattgaaggc tttgaataca
     1921 ggtcttatcc tgttcgcttt tgggatatat ttggtgaagg aggacatccc gtccgcacca
     1981 cgatttccga aatgggtgca atattgcttt caagattgtt agagctcaat gagacccagt
     2041 cgggaatcat gaatattgtt tttaggatag cggatgacaa agggctgtta ctgattgatt
     2101 tgaaggattt acgtgcaatg ttgcaatatg taggagagaa tgccaaagaa tttaccatta
     2161 agtatggcag catttctact caaagtgtag gtgccattct tcgaaagctg attgctttag
     2221 aggatcaggg gggcaattac ttttttggcg aacctgattt gaatattttt gactttatca
     2281 atgtgacatc tgagggacgt ggttatatta atattctaca tagtgtcaag ctggttcaaa
     2341 acccgatact ttatacaacc ttccttttgt ggctcttgtc ggagttattc gaaagccttc
     2401 ctgaagtaga cgatgtaaaa ataccgaaaa ttgttttctt ctttgatgaa gcacatctgc
     2461 tttttaagga tgctcccaag gtattaatac aaaaaataga gcaggtagta aggttaatcc
     2521 gttc
//
LOCUS       DAOOJC010000362         2523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_32279_length_2523_cov_2.575770, whole genome shotgun sequence.
ACCESSION   DAOOJC010000362 DAOOJC010000000
VERSION     DAOOJC010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32279_length_2523_cov_2.575770"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="PLA01_08965"
     CDS             complement(<1..954)
                     /locus_tag="PLA01_08965"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPD01462.1"
                     /translation="MELSIKALSISPSSTLAIDAKAKKMKAEGIDIIGFGAGEPDFDT
                     PAHIKEAAKKALDEGFTKYTPASGTLELKQAICNKFKRDNGLDYKPSNIVVSNGAKHS
                     LVNAFQAICNPGDEVIITTPAWVSYPEMVKLADAVSVYINTTEEEGFKFTIDKLEKAI
                     TDKTKAIIMNSPSNPTGMIYSEEELKAVADLAVSKGIYVISDEIYEKLIYDGYKHVSI
                     ASFNDKIKDLTIVVNGVSKSYSMTGWRIGYTASNEQIAKVMSNMQSHATSNPNSIAQK
                     AALAALEGPQEIIDTMAAEFVKRRDYMVDRINSIEGLSCIKP"
     gene            complement(1218..2156)
                     /locus_tag="PLA01_08970"
     CDS             complement(1218..2156)
                     /locus_tag="PLA01_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPD01463.1"
                     /translation="MIFVDAHCDTITKIMENGENLVKNSCHIDIERQKTLGKSVQFFA
                     AFIDPAYCQAYALKRALQIIDEFYRQVEINKDDIMLCCNYNDIMKAVDGNKIAAVLSI
                     EGGEALQGDLGVLRMLYRLGVRSICLTWNYRNEIADGVKDGESGGGLTPFGKKVIREM
                     NELGMLVDLSHLSEKGFWDVMECTGAPVIVSHSNAKKLCSHGRNLTDEQIDAVKYNGG
                     VIGINLYPDFLKDSKDASISDIIRHIEYISGLIGSDHIGLGADFDGVESLPEGIGGVQ
                     DVGKIIDELSKLNYSSQDIEKFAGGNFLRVIKEVLR"
BASE COUNT          717 a          560 c          394 g          852 t
ORIGIN      
        1 gggctttatg caggacaatc cttcgattga gtttattctg tcaaccatgt aatctcttct
       61 ctttacaaac tctgcagcca ttgtgtctat tatctcctgc ggtccttcaa gtgctgcaag
      121 ggctgctttc tgagctatag agttcggatt ggatgtagca tggctctgca tgttgctcat
      181 tacttttgca atttgctcat ttgaagcggt gtagccgatt ctccatcccg tcatggaata
      241 agacttggat actccgttaa ccacaatggt gaggtccttt atcttatcgt tgaaggatgc
      301 tatgctgaca tgcttgtatc catcataaat cagtttttca taaatctcat cggaaataac
      361 ataaatcccc ttgcttacag ccagatctgc taccgctttc agttcctcct cgctgtaaat
      421 catacctgtc gggttgctgg ggctgttcat tataatcgcc ttggttttgt ctgtaatagc
      481 cttttccagc ttatctattg taaacttgaa gccttcttct tccgttgtat tgatgtacac
      541 cgatactgca tcggcaagct taaccatttc cggatagcta acccatgcag gagttgtaat
      601 tataacttca tcgccgggat tgcaaattgc ctgaaacgca ttaaccaatg agtgctttgc
      661 accgttgctt acaacaatat ttgaaggctt gtagtcaagc ccgttgtccc ttttaaactt
      721 gttgcaaatt gcctgcttta attccagggt accggaagca ggtgtatact tcgtgaatcc
      781 ttcatccaat gctttttttg ccgcttcttt tatatgggca ggtgtgtcaa aatccggctc
      841 gcctgctcca aatccaatta tatcaatacc ttcggctttc atcttttttg cttttgcatc
      901 tatagccaat gttgatgatg gactaattga aagagcctta attgaaagtt ccattttaaa
      961 ccctccaaat ttcaattatt atgatattca ttttaatata taatattcaa ttttgcaagt
     1021 taatgatatg aataatgcaa aattattttt aaacattatt gtgatctagc aataaaaagt
     1081 aatataatat caaaatgcaa gtttgaaatt tcattttttc aatatgaaag aaattgtata
     1141 caatcacaaa aaatagaccg aagtaaaaaa tactccggcc attattagga aggtcaaaaa
     1201 tgaaagaccc aagattatta tcttaaaact tctttaatta ccctcagaaa attccctcct
     1261 gcaaacttct ctatgtcctg tgaagaataa ttcaattttg aaagttcgtc aattatcttt
     1321 ccaacgtcct gtactccgcc aattccttct ggcaagcttt caacgccgtc aaaatcggct
     1381 ccaagtccaa tgtgatccga tccgataagc cctgaaatat actcaatatg tcttattata
     1441 tcacttattg aagcatcctt cgaatccttt aaaaaatcgg gatacaggtt tattcctatt
     1501 actcctccgt tgtattttac cgcatctatt tgctcgtcag ttaagtttct gccatgggaa
     1561 cacagttttt tagcatttga atgggacaca attaccgggg caccggtaca ctccatcaca
     1621 tcccagaatc ctttttccga aaggtgggac aggtccacga gcattcccaa ttcattcatc
     1681 tctcttatga cctttttccc aaagggagta agtccgccgc cggattctcc gtcctttaca
     1741 ccgtcggcaa tctcatttct gtagttccat gtgaggcata tgcttcttac cccaagcctg
     1801 taaagcatcc ttaaaacccc cagatctccc tgaagggctt ctcctccttc aattgaaagc
     1861 accgcagcaa ttttattgcc gtccacagcc ttcattatat cattgtaatt acaacataac
     1921 attatatcgt ccttattaat ctccacctgc ctgtaaaact catctataat ctgcaaagcc
     1981 ctttttaaag cataagcctg gcaataggcc gggtcaataa aggctgcaaa aaactgaaca
     2041 ctcttcccta aagtcttttg tctttctata tcaatatggc aactattctt aacgaggttt
     2101 tctccgtttt ccattatctt tgtgattgta tcgcagtggg catcaacgaa aatcatgtta
     2161 caccttccca tacacattga ttctaaaaaa acacattaat acaaaacaaa gcaaacaccc
     2221 ttttatattt cctgaatact gtaaatttca acaataattc ataggcttat ttatgctttt
     2281 cattttatca aaaatttcaa taataatcta tgcattctga ttatattata tgttataatt
     2341 ggttttgagc ctgccgagtt aaattccgat accttttttt cggcaaaata aaatattaag
     2401 gagcggtaac ccctatgttc aataaagaca ggaaaatccc ttatattaag tacttaccgt
     2461 taattactat ttcatttatt cttttcaagt taattaacaa tactggtgca cttatgggcg
     2521 gaa
//
LOCUS       DAOOJC010000363         2515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_32426_length_2515_cov_3.564634, whole genome shotgun sequence.
ACCESSION   DAOOJC010000363 DAOOJC010000000
VERSION     DAOOJC010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32426_length_2515_cov_3.564634"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..1435
                     /gene="purB"
                     /locus_tag="PLA01_08975"
     CDS             8..1435
                     /gene="purB"
                     /locus_tag="PLA01_08975"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPD01464.1"
                     /translation="MKNTYESPFNSRYASNEMQELFSPDMKFKTWRRLWIALAEAEKE
                     LGLNITDEQIEELKKYKDDINYDVAEIKEKEFRHDVMAHIHAYGEQCPNARAIIHLGA
                     TSCYVGDNTDIIIMTEALKLIKKKLLAVIAGLADFALKYKDLPTLGFTHYQPAQLVTV
                     GKRATLWIQDLLIDLEDLEHLLSNMKLLGSKGTTGTQASFLNLFDNDHEKVRKLDELI
                     AKKMGFDCVFPVSGQTYTRKLDSRVLNVLSGIAQSAYKFGNDLRLLQSMKEIEEPFEK
                     SQIGSSAMAYKRNPMRSERICALARYVIVDALNPAITASTQWFERTLDDSANKRISIP
                     EGFLAVDAILGIYINITDGLVVYPKVIEKHILDELPFMATENIMMEAVKRGGDRQELH
                     ERIRVHSMEAAKQVKVEGKNNDLINRIAADKMFGLNIEEINAVLDPKNYVGRSPQQVE
                     EFIDEYVKPVLEKNKVENIKVDLRV"
     gene            1478..2215
                     /locus_tag="PLA01_08980"
     CDS             1478..2215
                     /locus_tag="PLA01_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPD01465.1"
                     /translation="MINKYSNEPLYSQLKNIIIEKIEKGEYPSGSKIPSEQELCDFYD
                     ISRPTVRQAISELTNSGYLYKEKGKGTFVSNPKTVVNAKSYTGFTDSILDNDMAGERN
                     IISIETLTNRDLRLLDEVFSVGSNQVVDFACFSYTGKINDDLISLNTSYIPLNLFPDI
                     IEDVKKGKSSFDILKGKYPLLPAKTRSTLEVVYTEQFETQYLMVPPGQALIKISNIIS
                     SKSGQVVEYVISKYRADRCRLLFENSK"
     gene            complement(2275..>2515)
                     /locus_tag="PLA01_08985"
     CDS             complement(2275..>2515)
                     /locus_tag="PLA01_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrrolo-quinoline quinone"
                     /protein_id="HPD01466.1"
                     /translation="TIESDYYCWSSPVAVYTPEGKSYIIQCDSGGFMYLIEGISGKIL
                     DKIPLQANIEGSPAIYEDMIVVGTRGQQIWGIKIK"
BASE COUNT          802 a          476 c          567 g          670 t
ORIGIN      
        1 gaatactttg aaaaacactt acgaaagtcc atttaactct agatacgcaa gcaatgaaat
       61 gcaggagctt ttttctcccg atatgaagtt caagacatgg aggaggctct ggattgccct
      121 tgcagaagcg gaaaaggagt tgggacttaa cattaccgat gaacagattg aagagctgaa
      181 aaaatacaag gatgatataa attatgatgt ggcagaaata aaggagaaag aattccgcca
      241 tgatgtaatg gctcatatac acgcttacgg cgaacagtgc ccaaatgcca gagccataat
      301 ccatcttgga gctacatcct gttacgttgg cgacaatact gatataataa ttatgaccga
      361 ggcattaaag ctcataaaga agaaactttt agccgtaata gcgggactgg cggattttgc
      421 attaaaatac aaagaccttc ccacactggg ctttacccac tatcaaccgg cacagcttgt
      481 tactgtcgga aaaagagcca ccctatggat tcaggatctt ttgatagacc ttgaggacct
      541 tgaacatctt ctttccaaca tgaaactgtt gggctcaaaa ggaactacgg gtacacaggc
      601 cagtttcttg aacctgtttg acaatgacca tgaaaaggtt cgaaaactcg atgaacttat
      661 tgcaaagaag atggggtttg actgtgtgtt ccccgtatcg ggacaaacat atacccgaaa
      721 gcttgacagc cgtgttctta acgtactgag cggtattgca cagagtgcat acaaatttgg
      781 aaatgactta aggcttttgc aaagcatgaa ggaaattgaa gagccctttg agaagagtca
      841 aatcggttct tcggcaatgg catacaagag aaatccaatg aggtccgaga ggatatgtgc
      901 tttggcaagg tatgttatag ttgatgcact taaccctgcc attaccgcat cgacccagtg
      961 gtttgaaagg acccttgacg actctgcaaa taaacgtatc agcattcccg aaggattcct
     1021 tgcggtagat gccatattgg gtatatacat caatattacc gatgggctgg tggtttaccc
     1081 caaggttatt gaaaagcata ttttagatga acttcccttc atggcaacgg aaaacataat
     1141 gatggaagca gtaaaacgcg ggggagacag gcaggagctc catgagcgta tcagggttca
     1201 ttccatggaa gctgcaaaac aggttaaggt agaaggaaaa aataacgatc ttataaatag
     1261 aatagctgct gacaaaatgt ttggtctcaa cattgaagag attaatgccg tacttgatcc
     1321 aaaaaactat gtgggaagat cgccgcagca ggtggaggag tttatcgacg agtatgtaaa
     1381 gcctgttctt gaaaaaaaca aggttgaaaa tataaaggtt gatctaaggg tataatatgt
     1441 gtttgtttca gatttgagga tacaggagga atcagctgtg attaataagt atagtaatga
     1501 acctctatac tctcaactaa aaaacataat tattgaaaaa atcgaaaagg gtgagtatcc
     1561 gtccggttca aaaataccat cggagcagga actgtgtgat ttttacgata tcagccgtcc
     1621 tacagtcaga caggccataa gtgaactgac aaacagcggt tatttatata aggaaaaggg
     1681 aaaggggaca tttgtttcga atcccaagac agtagttaat gccaagtcat atacgggttt
     1741 taccgattca attcttgaca acgatatggc cggtgaaagg aatataatat ccatagagac
     1801 attgaccaac agggatctgc ggttgcttga cgaggtattc tctgtcggta gtaatcaggt
     1861 tgttgatttt gcgtgtttta gttatacagg aaaaatcaat gatgacttga tttcactgaa
     1921 tacctcgtat ataccgctaa atctttttcc cgatattata gaggatgtca agaagggaaa
     1981 atcctccttt gatattctga agggtaaata tccgctctta cccgcaaaga caagaagcac
     2041 tcttgaggtg gtgtataccg agcaatttga aacacagtat ctgatggttc caccgggaca
     2101 ggcccttata aaaatatcca atataatctc ctcaaagagc ggccaggtgg tggagtatgt
     2161 gatttcaaag tacagggcag acagatgcag gttgttgttt gaaaacagca aatagttttg
     2221 tgtgcatttc aaacattatt aagcaatctg caggaggcct gtcgatattt ttcttcactt
     2281 gattttaatt ccccaaatct gctgtccccg ggttcccacc acaatcatat cctcatatat
     2341 tgccggagaa ccttcaatat ttgcctgaag gggtatttta tccagaatct ttccgcttat
     2401 gccttctatg agatacataa atcccccgga atcgcattgt atgatgtagg acttgccttc
     2461 gggcgtatac acagctacag gggaactcca gcagtaatag tccgattcta tggtc
//
LOCUS       DAOOJC010000364         2513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_32457_length_2513_cov_3.494304, whole genome shotgun sequence.
ACCESSION   DAOOJC010000364 DAOOJC010000000
VERSION     DAOOJC010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32457_length_2513_cov_3.494304"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PLA01_08990"
     CDS             complement(<1..511)
                     /locus_tag="PLA01_08990"
                     /inference="COORDINATES: protein motif:HMM:NF025717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HPD01467.1"
                     /translation="MKLVYICSPLRGAIEENIKKAHRYCEYAAGCDVIPLAPHTIFTA
                     YLQDTIPEQRVQGLKMGLELLKRCDEIWVCGDEISQGMQGEIDLATKLHIPTIYVLGH
                     HIEERLKIRQNSKALSIDDCIAGSDRYNYENKILVLNPEALISNCRTAENSLWVAYNG
                     FGCTYGARGQ"
     gene            complement(588..>2513)
                     /locus_tag="PLA01_08995"
     CDS             complement(588..>2513)
                     /locus_tag="PLA01_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPD01468.1"
                     /translation="RSFKNSNEIKVYPIPQLVFTLPKAAHTDDIITVNITTADMDGLT
                     VKWYVNNMQGFQDWDTYIDGTLGNNGGDIRFKLAGNYGIEASVTDETGRVFQFNCENK
                     IEILPVLSIEFNLPQIAHTDTDIDIRTTGDMGVLPIEWSLMRNGQPAKWDESVNGTMN
                     EQGGKIRLMQEGEYLLTATMTDALGRIFSYSAQISVYPLINCDFSMPSSVRTGVPFEV
                     TVSQAANPGGKNIVWSLEKDGQSIPLDGNIQGNLGNHGGTVTISTPGNYILSATVVDE
                     LGRTFTSKQSVEVINTAPTKPVANANVTRTFSSGKFLVEIYANSSDPDGDDINYEYSG
                     KAEDNYYTVGTHTVRVRAKDNFGGASDWTDVTFTVSNATPTKPTIVADVTRNAKDGKF
                     LVNVSVTSTDADGDEIAYEYDGKAQDNYYPVGTHTVRARAKDNYGGVSEWSEVTFTIQ
                     NSAPTAPVITRTPAGNSVTPGTPVTITATSTDADGDAITYVWENRPAQTYVYPLGKNV
                     VKVKAVDSTGAESTWSAIVFFVSDSTNGGGMVLTGPDSVIMENGLEGATITQYTFTVP
                     PVSGHSGSDFGRVRGYNIKTNTWEQLDYRTTTNGITFQRNLPAGTYSKLEFYYYTNHN
                     CMYNKSNITYSVNYYFE"
BASE COUNT          657 a          587 c          480 g          789 t
ORIGIN      
        1 cctgacctct cgccccataa gtacagccaa aaccgttata agcaacccac aaactgtttt
       61 ctgctgtccg gcaattacta atcaatgctt caggatttag caccaaaatc ttattttcat
      121 agttatatcg gtcacttcct gcaatacaat catctatgct gagtgctttg ctgttctgtc
      181 tgatttttaa cctctcctca atatgatggc ccagaacata aatcgtcggg atatgcagtt
      241 tggttgccaa atcaatctca ccctgcattc cctgcgatat ttcatccccg cacacccaaa
      301 tttcatcaca acgctttaac agttccaacc ccatttttaa gccctgtacc ctctgttcgg
      361 gaatggtatc ttgcagataa gctgtaaaaa tcgtatgggg tgccaatggt attacatcac
      421 atccggcagc atactcgcag tatcggtgtg ctttttttat attttcctca attgctcccc
      481 ttaaagggga acagatatat accagtttca agccatttca cctccgttta tataaggcaa
      541 ataaccgccc tgatgtttta tcaaggcggt tatcatgatg attttactta ttcaaaatag
      601 taatttacag aataggtaat gttagacttg ttatacatac aattatggtt agtatagtag
      661 tagaactcca gcttggaata agtgcctgcc ggtagatttc tttggaaagt aatgccatta
      721 gtggttgttc tgtaatcaag ctgttcccat gtatttgttt taatgttata tcctcttact
      781 cgtccaaaat cacttccgct gtgtccgctg acaggcggta cagtaaaggt gtactgcgta
      841 atagtagcgc cctccaatcc attttccatg atgactgaat ccggaccagt cagcaccata
      901 ccgccaccat tggtagaatc tgacacaaaa aatacaatag ccgaccacgt agattcagca
      961 ccggtactgt caactgcctt aaccttcact acatttttgc ccagtggata aacataagtt
     1021 tgagcaggac ggttttccca aacataggtt atggcatccc catctgcatc agtagaagtt
     1081 gctgtaattg taacaggtgt acctggtgta acgctgttgc cggctggtgt tcttgtaatc
     1141 accggtgccg taggtgcaga attttggata gtaaaggtca cttctgacca ctcagaaaca
     1201 ccgccataat tgtcttttgc tcttgctcta acagtatgag tacctacagg atagtaattg
     1261 tcctgagcct ttccgtcgta ttcataggca atttcatcgc cgtcagcatc ggtgctggtt
     1321 acagagacgt tgacaaggaa tttcccgtct tttgcatttc gggtaacatc agccacaata
     1381 gttggctttg tcggtgttgc attgctgaca gtaaaggtta cgtctgtcca gtcggaagca
     1441 ccgccaaagt tatcctttgc tcttactctt accgtatgag taccaacggt ataatagtta
     1501 tcttcagcct ttccactgta ttcataattg atgtcatctc catcggggtc gctgctgttg
     1561 gcataaatct caacaagaaa tttaccacta ctaaatgtac gggttacatt ggcgtttgca
     1621 accggctttg tgggagctgt attaattact tcaacgctct gttttgaggt gaatgttctg
     1681 ccaagctcat caacaacggt ggcactcaaa atatagtttc ccggtgtact gattgtcaca
     1741 gttccgccgt ggtttcccaa attaccctgt atattgccat ccaacggtat agattgcccg
     1801 tctttttcaa ggctccaaac aatatttttg ccaccaggat ttgcagcctg actgactgtt
     1861 acttcaaatg gtactcctgt acgtacactg ctcggcatcg agaaatcaca atttatcaat
     1921 gggtaaacgc tgatttgtgc gctatagctg aaaattctgc ctaaagcatc ggtcattgtt
     1981 gcggtaagga ggtattcacc ttcctgcatt agacggattt ttccaccttg ttcattcatc
     2041 gttccattga ctgactcatc ccatttagca ggctgaccgt ttctcattaa actccattca
     2101 atcggtagca ctcccatatc gcctgtagtg cgaatatcaa tatccgtatc tgtatgggca
     2161 atctgaggaa gattaaattc tatggagagg acagggagaa tctcgatctt attttcacaa
     2221 ttgaattgga atactcttcc ggtttcatct gtcacacttg cttcaatgcc atagtttccg
     2281 gcaagtttga aacgaatgtc tccaccatta ttcccaagtg tgccgtcaat ataggtatcc
     2341 caatcctgaa aaccttgcat attgttcaca taccatttaa ctgtcaatcc gtccatatcg
     2401 gcagttgtaa tgttgacggt aataatatca tcggtatgag cagctttggg caaagtaaat
     2461 acaagctgcg gaatcggata taccttgatt tcattactat ttttaaaact tct
//
LOCUS       DAOOJC010000365         2503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_32646_length_2503_cov_2.912990, whole genome shotgun sequence.
ACCESSION   DAOOJC010000365 DAOOJC010000000
VERSION     DAOOJC010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32646_length_2503_cov_2.912990"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /gene="aroC"
                     /locus_tag="PLA01_09000"
     CDS             complement(<1..790)
                     /gene="aroC"
                     /locus_tag="PLA01_09000"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPD01469.1"
                     /translation="MVGNSFGRIFRVTTCGESYAGGFRKNPDIPEELYGGLMTIVDGV
                     PPGIKLTSELIQEELDKRKPGKSALDTPRKESDRVFIFSGVMENDITTGAPVGMIIPN
                     RVIEDEHLKKHMSYNEVIRPGQAGYTFFKKYGQFADNIGAGRASGRETASRVAGGAVA
                     KAVLDTMGIDVIAFVTEIHGIKAENITYETAKANYRKNDLNCPDLNKAKEMMDELLKI
                     KAEGDSVGGVVEIIARGVPAGLGEPVFDKLQATLSHAIMSIGAIK"
     gene            complement(784..1335)
                     /locus_tag="PLA01_09005"
     CDS             complement(784..1335)
                     /locus_tag="PLA01_09005"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPD01470.1"
                     /translation="MKTNSIVLIGMPGSGKTTVGQPLSQALGMSFIDTDMIIFEKENK
                     PLKDIVAQDGLDRFLQIQEQTLLELDLNNRVISTGGSVVYNKTAMEFLKKNGIVVYLK
                     QELEELEKRLAPGRRLARNSEQGFADIYAEREPLYEKYADVTIDCTNKSVDSIVTEIT
                     SIYNEFKTRSNYIYEIKGEKVGW"
     gene            complement(1527..>2503)
                     /locus_tag="PLA01_09010"
     CDS             complement(1527..>2503)
                     /locus_tag="PLA01_09010"
                     /inference="COORDINATES: protein motif:HMM:NF025857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HPD01471.1"
                     /translation="TINNTVYADLNGDGEVNSTDFTLLKRYLLRKIKEFPVNGIRPDA
                     VRLDDLMGASVNTITNRFGQPDRIDLSRYGFDWYIYNNDLAKYLQIGIKDGKTVGIYS
                     NSLYYKLNDNIGIGTPKSTVEEELGTPLSSITKGSTSYRMDNKDEWKVYKKNNEYYTY
                     LFYNLQSDIEVTAFMMIDYATEQSLKGFYGTPSDRLRKSYEMELFDITNAYRIRHGKD
                     PFKWSDEMAALSRAFCKDMVEKGYFDFTDTNGQVVSDRMRNAGINYNSVSELIAKGQM
                     DSMFVIESWMKNRSSTLLGGHEYVGIGINMESDGCVVYVYDLYIPSKY"
BASE COUNT          671 a          559 c          413 g          860 t
ORIGIN      
        1 cttttatggc accgatagac attattgcat gggacagtgt tgcctgaagc ttgtcaaaaa
       61 ccggttcacc aaggcctgcg ggtactcccc gggctattat ttccacaacg ccgccgacgg
      121 aatctccttc tgctttgatt tttaacaact catccatcat ttccttggct ttattcagat
      181 cagggcaatt taagtcattt tttcggtaat tggcttttgc cgtttcataa gtaatgtttt
      241 ctgcttttat tccatgtatt tcggttacaa acgcaatcac gtcaataccc attgtgtcta
      301 aaacagcctt tgcaactgct cccccggcaa cccgtgaggc agtttccctg ccggaagccc
      361 ggccggcacc gatattatcg gcaaactgac cgtatttctt aaaaaaggta tagccagcct
      421 gacctggtct tattacctca ttgtaactca tgtgcttttt taaatgttca tcctcaataa
      481 ctctgttagg aataatcatt cccacaggtg caccagtggt aatatcattc tccataactc
      541 cggaaaatat aaatactctg tccgattctt ttcttggtgt atccaaggct gatttgccag
      601 gttttctttt atccagttct tcctgtataa gttcacttgt aagcttgatt cctgggggaa
      661 cgccatctac aatagtcatt aatccgccgt ataattcttc cggtatgtca gggtttttac
      721 gaaagccacc tgcataagac tccccacagg tagttaccct gaatattctg ccaaatgaat
      781 ttcctaccat cctaccttct ctcctttgat ttcataaata taattacttc tggttttaaa
      841 ttcattataa atactcgtta tttccgttac tattgaatca acacttttat tagtgcaatc
      901 aattgttaca tcggcatatt tttcgtaaag tggttctctt tctgcgtata tatcggcaaa
      961 cccctgttcg ctgtttcttg caagccttct tccgggagca agcctttttt caagttcctc
     1021 caactcctgc ttaagataaa caacaatacc gttcttcttg aggaactcca ttgcagtttt
     1081 attatataca acgcttccgc cggtagatat aacccggttg tttaaatcca gttccaaaag
     1141 tgtttgctct tgaatttgaa gaaacctgtc gagcccatcc tgggcaacaa tatccttgag
     1201 cggtttgttt tctttttcaa aaattatcat atcggtgtca ataaaactca tgccaagagc
     1261 ttgtgaaagc ggctgtccga ctgtagtctt accggaaccg ggcataccga ttaaaactat
     1321 gctgtttgtt ttcataatga tctcccttaa agtaaaacgc ttttataagc ttagacaacg
     1381 caatatgcaa aaacatattg cgttgtccat atcactaatg ttttatatat agtattatac
     1441 acataaactg acagaggtcc aagataaatt ttaaaatttt tcttaccttt cttactggtt
     1501 tgggaatctg ccaaataata ttttatctaa tacttactcg ggatatacaa atcataaaca
     1561 tagacaacgc atccgtcact ttccatgtta atacctatac cgacatattc atgacctccc
     1621 agtaaagtgg aacttctgtt tttcatccag gactcaatta caaacatgct gtccatctgt
     1681 ccttttgcaa tgagttctga aacggaattg taattaattc cggcatttct cattctatcg
     1741 gaaacgacct gaccgtttgt gtcggtgaag tcaaaataac ctttttcaac catgtccttg
     1801 caaaaagctc ttgataatgc tgccatttca tcggaccatt tgaaaggatc ttttccatgt
     1861 cttatcctat atgcattggt gatatcaaaa agctccattt catagctttt acgcagtcta
     1921 tcactgggag tgccataaaa tccttttaag gattgctctg tggcataatc aatcatcata
     1981 aaagctgtaa cttcaatgtc actctgcaga ttataaaaaa gatatgtata atactcatta
     2041 ttttttttgt ataccttcca ttcatccttg ttgtccattc tgtaactggt acttccctta
     2101 gtgatgcttg ataaaggggt tccaagttcc tcttccactg tggatttagg tgttccgatt
     2161 ccaatattgt cattgagctt gtaatacaga gagtttgaat atattcctac ggtcttgccg
     2221 tcttttatgc caatctggag atatttggcg agatcattat tgtatatata ccaatcaaag
     2281 ccgtatctgc ttagatcaat gcggtccggt tgaccgaatc tgtttgttat ggtatttacc
     2341 gatgccccca ttaagtcatc caaccttaca gcatctggtc ttatgccgtt aacgggaaat
     2401 tctttaattt ttcgaagcag gtaccttttt agcaatgtaa aatcggtaga atttacttcc
     2461 ccatcaccat ttaaatctgc atatacagtg ttattaatag tag
//
LOCUS       DAOOJC010000366         2503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_32647_length_2503_cov_2.883987, whole genome shotgun sequence.
ACCESSION   DAOOJC010000366 DAOOJC010000000
VERSION     DAOOJC010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32647_length_2503_cov_2.883987"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..388)
                     /locus_tag="PLA01_09015"
     CDS             complement(47..388)
                     /locus_tag="PLA01_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HPD01472.1"
                     /translation="MIKIKVIRDSANLIRGFTVKGHAGFSEAGSDIVCAAVSALAYTA
                     AGALLEFTGINGHTEKNGYMKCVVPDDIPKDKEHKAEIILETIILGFKQIEHSYGRYV
                     SVLDKEVLTDD"
     gene            complement(403..714)
                     /gene="rplU"
                     /locus_tag="PLA01_09020"
     CDS             complement(403..714)
                     /gene="rplU"
                     /locus_tag="PLA01_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPD01473.1"
                     /translation="MYAIIETGGKQYKVNEGDVVFVEKLLNEEGSSVTFDKVVAVSKD
                     GNASFGDPFIANATVTGKVLGHGKDKKIIVFKYKSKKGYRKKQGHRQPYTKVQIEKIN
                     A"
     gene            complement(821..2314)
                     /locus_tag="PLA01_09025"
     CDS             complement(821..2314)
                     /locus_tag="PLA01_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPD01474.1"
                     /translation="MVSEIIVDVGLNERRVALLEDRELVEIFIERDGEERLVGNIYRG
                     RVASVLPGMQAAFIDIGYEKNAFLYVGDAVSQKEFSEDEDEMYNDVKSYNIEEILRPG
                     QEITVQVTKEPIGTKGPRVTTHITLPGRQLVLLPNADYVGISRRIEQEEERAKLKKIA
                     EKIKPENMGLIVRTVSEGKREDDFKSDINFLVKLWKKIKQKEQNGPIPRCLHKELSVI
                     YRAVRDIFTWNIDRFVINDRQEYNKVLELVEMLSPALKLRVEYFNKNIDLFEHYQIDA
                     MIQKALAKKVWLKCGGYLIIERTEALTVIDVNTGKYVGVNNLEDTVLKTNLEAAKEIA
                     KQIRVRDVGGIIIIDFIDMHEPEHQRQVIEILKQVLKKDRTKTTVVGMTGLGLIEMTR
                     KKVREGLESVVLYNCPYCEGSGRILSPESVARDVEKRIIKYLTMTIANAILVEVYPTV
                     AEVLQDGANDNLARIQNSFNKKVVIKPSLEVKHDEIKIKEIDIDSLV"
     gene            complement(2324..>2503)
                     /locus_tag="PLA01_09030"
     CDS             complement(2324..>2503)
                     /locus_tag="PLA01_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01475.1"
                     /translation="KMLLAAGSAANLKPEFVIKAFADYSGYGFDILKIHRSGLFTGDK
                     DKLMDPLNGSVILSI"
BASE COUNT          681 a          627 c          349 g          846 t
ORIGIN      
        1 tgtactaccc attccttttt tatgggcaaa caactgcaaa tttactttaa tcatcggtta
       61 acacctcctt atctaaaacc gatacatatc ttccatacga atgttcaatt tgcttaaaac
      121 ccaatataat tgtctcaagg ataatttcag ccttatgctc cttgtctttc gggatatcat
      181 ccggaacaac gcatttcata tatccgtttt tttccgtatg accattgata ccggtaaact
      241 ctaagagtgc acctgctgcc gtatacgcaa gggctgagac cgccgcacaa actatatcac
      301 ttcctgcttc cgaaaaccct gcatgtccct ttaccgtaaa gcctcgaatc aaattcgcac
      361 tatccctgat gacttttatt ttaatcattt tttacttcaa ccttaagcat ttatcttttc
      421 aatttgtact tttgtataag gctgtctatg accttgcttt tttctgtatc cttttttgga
      481 cttatatttg aaaacaataa ttttcttatc ctttccatgt cccagaactt tacctgtaac
      541 agttgcattt gcaatgaaag gatcaccaaa gctagcattt ccgtccttcg acacagcaac
      601 aaccttgtca aaagtcactg aagatccctc ttcattgaga agcttctcaa caaaaacaac
      661 atctccctcg ttaaccttgt actgttttcc gcctgtttcg attatagcgt acataaaaac
      721 acctccctac ccagtctcgc cgataaaggc agtatcaata ataccagaac tattgatagc
      781 atttacaaaa cctgtaccga gcggctaccg aagtatttta tcacacaaga gagtcaatgt
      841 caatctcttt tatttttatc tcgtcatgct taacttccag tgaaggcttg attaccactt
      901 tcttattaaa tgaattctgt attcttgcca gattgtcatt ggctccatcc tgtaaaacct
      961 cagccactgt cggatagacc tcaaccaata tggcatttgc tatggtcatt gttaaatact
     1021 ttattattct tttttcaaca tcccttgcca ccgattccgg tgacagaatt ctgccggaac
     1081 cttcacaata tggacaatta tacaacacta cggattcaag accctctctt accttttttc
     1141 ttgtcatttc aataagcccg agtcctgtca ttcccacaac ggtagtcttg gtacgatcct
     1201 ttttcaacac ctgctttaat atttcaataa cctgccgttg gtgctcaggt tcatgcatat
     1261 cgataaaatc aatgataata atgcctccga catcccttac cctgatctgc tttgcgattt
     1321 ccttggcagc ttcaagattg gtttttaaaa cagtgtcttc aagattgtta acaccgacgt
     1381 acttcccggt attaacatca ataaccgtca gtgcctcagt tctttctatt atgaggtatc
     1441 caccgcattt taaccatact tttttggcca gtgccttctg aatcattgca tcaatctgat
     1501 aatgttcaaa aaggtcaata ttcttgttaa aatactcaac cctgagtttt aatgcaggag
     1561 aaagcatttc aacaagctca agtaccttat tgtattcctg tctgtcattt ataacaaatc
     1621 tatcaatatt ccatgtgaaa atgtccctta cagccctata aattacgctg agctccttgt
     1681 gcaggcaccg tggaatggga ccgttttgtt ctttctgctt tatctttttc cagagcttta
     1741 ctaaaaaatt aatatcactt ttaaaatcgt cttctctctt tccctctgat acagttctaa
     1801 caataagtcc catattttca ggttttattt tttcagcaat ttttttgagc ttcgcccttt
     1861 cttcttcctg ttctatcctt cttgatattc ctacataatc ggcattgggc aaaagcacca
     1921 actgccgccc cggtaatgta atatgagtgg taacccgtgg tcctttcgtc ccaataggct
     1981 ccttcgtcac ttgaacagtt atttcctgcc ccggccttaa aatctcttca atgttatagc
     2041 ttttcacatc gttgtacatc tcatcttcat cttcagaaaa ctctttttga gatacagcat
     2101 ccccaacata tagaaaagca tttttctcat aaccaatatc aataaacgca gcctgcatgc
     2161 cgggcaaaac actggcaact cttcctctgt aaatattccc tacaagtcgt tcctccccat
     2221 ctctttcaat gaatatctcg accagttctc tgtcttcaag aagggctacc cttcgttcgt
     2281 taagcccaac atctacaatt atctcactaa ccattctacc acctcatata gataatatta
     2341 cactaccatt aagcggatcc attagtttat ctttatctcc cgtaaaaaga ccggaccgat
     2401 gaatttttaa tatgtcaaag ccgtaaccgg aataatccgc aaatgccttt attacaaatt
     2461 caggttttaa gtttgctgca cttccggcag caagcagcat ttt
//
LOCUS       DAOOJC010000367         2491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_32857_length_2491_cov_2.321839, whole genome shotgun sequence.
ACCESSION   DAOOJC010000367 DAOOJC010000000
VERSION     DAOOJC010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32857_length_2491_cov_2.321839"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..299)
                     /locus_tag="PLA01_09035"
     CDS             complement(42..299)
                     /locus_tag="PLA01_09035"
                     /inference="COORDINATES: protein motif:HMM:NF022741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HPD01476.1"
                     /translation="MAPKNDDKEKQNFALGRENYKLLAIGFAIIVTGFLLMLGGRSDD
                     PNVFSEKIFSFRRITLAPIVVLAGFIFEIWAIMKKPGNSAK"
     gene            complement(303..1181)
                     /locus_tag="PLA01_09040"
     CDS             complement(303..1181)
                     /locus_tag="PLA01_09040"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPD01477.1"
                     /translation="MKNKKSGFANAKLRGSYLTLVISVSLVLFLLGILGLVLINAKEL
                     SDYFRESLSFSVMLDDDAREADIRMLQKDLDAKPYVKSTVYVSKDEAASKMKEDLGED
                     FISFLGDNPLPPSIDVYLYAGYTSPDSVARIEKYVLEYPFVKEVYYEESLLFLINENV
                     RKVSLFLLVISTFLFLIAITIINNTIRLAIYSKRFLIKTMELVGATKSFIRKPFLIRS
                     VYHGVLAALIAMTLLMGLLYLIEKEFYMMFSFESTKLLIILCVVLIILGILINLVSTY
                     LSVNRYLVISEDKLYN"
     gene            complement(1191..2255)
                     /locus_tag="PLA01_09045"
     CDS             complement(1191..2255)
                     /locus_tag="PLA01_09045"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPD01478.1"
                     /translation="MKNVLLPDVLKERSSMENLDWKNLPFGYMKTDFNIRCFYKDGKW
                     GDLIVSESEYIDIHIAATGLHYGQEAFEGLKAYMGKDGRIRLFRWEENAKRMVSSAEG
                     IKMAPFPVERFKEAVFRVVHMNKRFIPPYGSGATLYIRPLLYGSGAEVGVKPSAEYVF
                     IVFVTPVGPYFKGGIKPVDMLICRDYDRAAPKGTGVFKVGGNYAASLRAGMVAKERGY
                     ASTIFLDAREKKYIDECGPANFFGIKNNTYITPKSESILNSITNMSLLELAQTLGIKT
                     ERRQVPVEELGEFTEAGACGTAAVISPIRKIFDPDKNLVYEYCRDGNPGPVTLKLYNK
                     LTAIQFGDDEDEFGWISIVD"
     gene            complement(2291..>2491)
                     /locus_tag="PLA01_09050"
     CDS             complement(2291..>2491)
                     /locus_tag="PLA01_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01479.1"
                     /translation="LGRDDFIYLSGKLLKERAEHGLEIGNEGWVEMQKHLLVSHDFLT
                     GTARLLRSVSIEEQINSLRDHN"
BASE COUNT          744 a          556 c          426 g          765 t
ORIGIN      
        1 aattgcttct aaaagattca taatctgcga aataattaga attatttggc ggaattcccg
       61 ggctttttca taattgccca gatttcaaaa atgaatcctg cgagaacaac tatgggtgca
      121 agagttatcc ttctgaagct gaaaatcttc tcactgaaaa cattgggatc gtctgatctg
      181 cctccaagca taagcaaaaa tcctgttaca attatggcaa atccgatagc gagtagcttg
      241 taattttccc tgccaagggc gaaattttgt ttttccttgt cgtcattttt aggtgccatg
      301 attcaattat atagtttgtc ttcagaaata acaagatatc tgttaactga taaataagtt
      361 gaaaccaggt tgatcagaat tcccagaatt atcagtacaa cacatagaat aattaggagt
      421 tttgtacttt caaaagaaaa catcatataa aactctttct caataagata taacaagccc
      481 atgagtaaag tcatcgcgat cagtgctgca agtaccccat gatatacact tcttataaga
      541 aatggctttc ttatgaatga ttttgtggct cccacaagtt ccattgtctt tataagaaac
      601 ctctttgaat agatagccaa ccttattgta ttgtttatta tggttattgc aataaggaaa
      661 aggaaagtgc ttataacaag aagaaacagg ctgacttttc tgacattctc gttaataagg
      721 aaaagtagtg actcttcata atagacttcc ttaacaaaag ggtattcaag aacatatttt
      781 tcaatccttg cgacactgtc agggcttgta tatccagcat ataggtacac atcaattgat
      841 ggaggcaatg gattatctcc gaggaaactt ataaaatcct cccccaggtc ttctttcatt
      901 tttgaagcag cttcatcttt tgatacataa acagtcgatt ttacataagg ttttgcatcc
      961 agatcctttt gaagcatcct gatatcagcc tccctggcgt catcgtcaag cattacagag
     1021 aaagagaggc tttccctgaa gtaatctgaa agttcttttg cgttgatcag tacaagtcca
     1081 agtattccaa gaagaaaaag gacaagtgaa acactaataa caagtgtcag gtatgatcct
     1141 cttaattttg cgttggcaaa tcctgatttc ttattcttca cagttgtttc ttaatcaaca
     1201 atagaaatcc atccaaactc atcctcatca tctccgaact gaatggcagt aagtttatta
     1261 tacagcttca gggtaaccgg ccccggattc ccatctctgc agtactcata gaccagattc
     1321 ttgtcaggat cgaatatttt tcttattgga ctgataaccg ctgcagttcc gcatgcccct
     1381 gcttcagtaa attcacccag ttcctcaacc ggcacctgcc gtcgctcagt ttttatacca
     1441 agagtctgag ctaactcaag caggctcata tttgttattg agttcaatat tgattccgac
     1501 tttggagtaa tatacgtatt gttcttaata ccaaaaaaat tggcgggtcc gcattcatcg
     1561 atatattttt tttccctggc atcaaggaat atagtgcttg catagcccct ttcctttgct
     1621 accatcccag ccctaaggct tgctgcatag tttcctccca ccttgaaaac gccggtgcct
     1681 ttaggtgctg cacggtcata atccctgcat ataagcatgt cgaccggctt tatgccacct
     1741 ttgaaatatg gtccgacagg ggttacaaac acaataaaaa cgtattctgc cgacggtttt
     1801 actcctactt cagcaccgct cccgtaaaga agagggcgaa tataaagagt tgcacccgaa
     1861 ccgtatggag gaatgaatct cttgttcata tgaacaactc tgaagacagc ttccttaaac
     1921 ctttcaacag ggaatggtgc catttttata ccctcagcag aagataccat cctttttgca
     1981 ttttcttccc aacggaacaa cctgatcctg ccatctttac ccatatatgc cttgagaccc
     2041 tcaaatgctt cctggccgta gtgcaggccg gttgcggcta tatgaatgtc aatgtattca
     2101 gattcagaaa caatcagatc tccccatttc ccgtccttat agaaacaacg tatattaaaa
     2161 tccgtcttca tgtaaccgaa tggcagattt ttccagtcta aattctccat actcgatctc
     2221 tcttttaaaa catccggtaa aagtacattt ttaatgataa tcttctatag tatattccta
     2281 aaatgaacta ttaattgtga tcccgcagtg aattgatttg ttcctcaatt gaaacacttc
     2341 gcaaaagcct ggcagttcct gtaagaaaat catggctaac aaggagatgc ttttgcattt
     2401 caacccatcc ttcatttccg atctccaaac catgttcagc cctctccttt aacagcttcc
     2461 cggacaagta aataaaatca tcacggccga g
//
LOCUS       DAOOJC010000368         2458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_33468_length_2458_cov_3.197670, whole genome shotgun sequence.
ACCESSION   DAOOJC010000368 DAOOJC010000000
VERSION     DAOOJC010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33468_length_2458_cov_3.197670"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PLA01_09055"
     CDS             <1..379
                     /locus_tag="PLA01_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPD01480.1"
                     /translation="VPDIRDKSISDAKKLLNQMGLKCRIEGEGYDDSTVIKDQTPKPG
                     ALLAKDSVVIVYTNKQEEEIMVKVPDVTNKTIDEATQSLNAAGLNIKVVGNGTAVRQK
                     VEPGTEVRQGEVIEVEFLFLDVH"
     gene            428..1876
                     /locus_tag="PLA01_09060"
     CDS             428..1876
                     /locus_tag="PLA01_09060"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010292343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPD01481.1"
                     /translation="MKLKELIKGLKVVELEGDMEIEIGSIAYDSRKAKEGSVFVCIEG
                     FKVDGHKYIPQAIENGTRAFIVQKEVEIPAGMSVVRVEDTRYALAGISDIFFGHPSGK
                     FNLAGITGTKGKTTTTYMIKSVLEEYGQKVGLIGTIANMIGDEMLPTDRTTPESYDLQ
                     ELFSEMVQKGVKSVVMEVSSHALDLHRVSCSEYDIGVFTNLSRDHLDFHKTFENYLEA
                     KIKLFGMCKKGLINIDNEFGRKVADSARCEVYTMGIDSEADVRAVDIVTHPNSVDFKV
                     VSPWFEGKISVSIPGKFSVYNALAAIGSCALMGVPFEYIQKGLAGVRVPGRSEIVDIG
                     KDYTVMIDYAHSPDSLENILTTVKAYAPGRVVCVFGCGGDRDKTKRPVMGRISGQLAD
                     FTIITSDNPRTEDPEMIINDIEEGIKTTGATYIKIVDRREAIKYALMNAKPKDIIVLA
                     GKGHENYIILKDKTIHFDEREVIREILNESIN"
     gene            1897..>2458
                     /gene="murF"
                     /locus_tag="PLA01_09065"
     CDS             1897..>2458
                     /gene="murF"
                     /locus_tag="PLA01_09065"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPD01482.1"
                     /translation="MENLNCQEIIRVVNGTLLSGDINTVFDNVSTDSRNIKQGDLFVP
                     LVGEKFDGHDYISPSFEKGALGSLTQKDTGVCCDRVLIKVSDTLKALRDLAAYYRQKF
                     NIPFVGITGSVGKTSTKDMVAGVLQRGFRVHKTQGNFNNEIGVPLTVFGLDPSHEACV
                     VEMGMSGFGEISRLTSIVKPDIAIITN"
BASE COUNT          774 a          394 c          650 g          640 t
ORIGIN      
        1 tgtaccggat ataagggata agagcataag tgatgccaaa aaacttttaa accagatggg
       61 attgaagtgt aggatagagg gagaaggtta tgacgacagt accgtcataa aagatcaaac
      121 accgaaaccc ggtgcactgc ttgcgaaaga ttctgttgtt attgtgtata caaataagca
      181 ggaagaggaa ataatggtta aggtacctga tgttactaat aaaaccattg atgaagctac
      241 gcagtcttta aatgctgccg gattgaacat taaagttgtc ggtaacggaa cagcggtgcg
      301 gcaaaaggtt gaaccgggaa ctgaggttcg ccagggagaa gtcatagagg ttgagttctt
      361 gttcctcgat gtgcactgat ggctttgtag actttgagag aagtaaattg gacgggaggt
      421 tttgtaaatg aaacttaaag agctcattaa gggattgaag gttgttgagc ttgaagggga
      481 tatggaaatt gagataggaa gtattgcata tgactcaaga aaggcaaagg aaggctcggt
      541 ttttgtatgt atagaaggtt ttaaggttga cgggcataaa tatatacccc aggctataga
      601 aaacggcacc agggcgttta tagtccagaa ggaagttgaa attcccgccg gtatgtctgt
      661 tgtcagggtt gaagatacca gatatgccct ggccggtatt tcggatatat ttttcggcca
      721 tccctcgggg aaattcaatc ttgcagggat taccgggact aagggaaaga ctactacaac
      781 ctacatgata aaatcggttc tggaagaata cggtcaaaag gttgggctga tcggaacaat
      841 agcgaacatg atcggcgatg agatgcttcc gacagataga acaacaccgg aaagctacga
      901 tcttcaggag cttttcagtg aaatggttca aaaaggtgta aaaagtgtgg ttatggaggt
      961 ttcttcccat gccttggacc tccacagggt aagctgcagc gagtacgata tcggagtttt
     1021 tacgaatctt tcacgggatc acctggattt tcataaaacc tttgagaact atcttgaagc
     1081 aaaaatcaag ctttttggaa tgtgtaaaaa aggattgatt aacattgaca atgaattcgg
     1141 aagaaaagtg gcagacagtg cccgttgtga ggtatataca atgggtattg acagtgaagc
     1201 ggatgtcagg gcggtggaca ttgtgaccca tcccaacagt gttgatttca aggtggtatc
     1261 tccctggttt gaagggaaaa tcagtgttag tattcccgga aagttcagtg tttataatgc
     1321 cctggctgca ataggaagct gtgcattgat gggagtgcct tttgagtata tacaaaaggg
     1381 actggcgggt gtcagggtac cgggaaggtc agaaattgtc gatataggaa aagactatac
     1441 cgttatgata gattatgccc attcccccga cagcctggaa aatattctga ctaccgttaa
     1501 ggcatatgca ccgggaaggg ttgtgtgtgt gtttggctgc ggaggagacc gcgataaaac
     1561 caaaaggccg gttatgggca gaatatcggg acaattggcg gattttacga ttattacctc
     1621 cgacaacccg aggacagagg acccggaaat gataataaat gatattgagg aaggaattaa
     1681 aactaccggt gctacatata taaaaattgt ggataggcgt gaagctataa aatacgcatt
     1741 aatgaatgca aagccaaaag acattattgt tcttgcaggt aaaggacatg agaattatat
     1801 aatactaaaa gataaaacaa tacattttga tgagagggaa gtaataaggg aaatactcaa
     1861 tgaatctatc aattaaattg cctggaggaa tattaaatgg agaatttaaa ttgtcaagag
     1921 ataataagag tagtaaacgg gactttgttg tctggtgata tcaacacggt atttgataat
     1981 gttagtactg actccagaaa cataaagcag ggagacttat ttgttcctct tgtgggagag
     2041 aagtttgacg ggcatgacta tatttcgcct tcttttgaga agggggcatt gggcagcctt
     2101 acacaaaagg acaccggtgt ttgctgcgac agagttttga taaaggtttc cgataccttg
     2161 aaggctctta gagatcttgc tgcatactac aggcagaaat ttaacattcc ctttgtaggt
     2221 attaccggaa gtgtaggcaa gacaagcaca aaagatatgg tggccggtgt actgcagagg
     2281 ggctttagag tgcataagac ccaaggcaat tttaataacg aaatcggtgt accccttacg
     2341 gtttttgggc ttgacccctc tcatgaggct tgtgttgttg agatggggat gagtggcttc
     2401 ggggagataa gccggctcac ttcaattgta aagccggata ttgcaataat aacaaaca
//
LOCUS       DAOOJC010000369         2454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_33544_length_2454_cov_3.221342, whole genome shotgun sequence.
ACCESSION   DAOOJC010000369 DAOOJC010000000
VERSION     DAOOJC010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33544_length_2454_cov_3.221342"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..>2454
                     /locus_tag="PLA01_09070"
     CDS             196..>2454
                     /locus_tag="PLA01_09070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPD01483.1"
                     /translation="MSDDKKELKVSIRNLVEFVLRCGDIDSSFGGTNRAVEGTRLHKK
                     IQKAQGEEYSAEVTLKTAVEFEGFSLVVEGRADGIINEKGCFTVDEIKTTTMPLDSID
                     EDFNPIHWAQAKCYAYIHALNENLNEINVRLTYCHLDTEEIKYLTKTFDFAELKKFFY
                     ELIEKYYIWAKFAYDWQINRNDSIKALNFPFARYRKGQRELAVAVYKTIEGGKKLFVK
                     APTGIGKTISTLFPSVKILGEGHISKIFYLTAKTITRSVAQEALSIMREKGLLIKNVT
                     LTAKEKICFMEKSNCKPEKCEYAKGHYDRINDAIMDVLQEECEINREVIEEYAKRHRV
                     CPFEMSLDLTLWADVIICDYNYAFDPRVYLKRFFSDAGGDYAFLVDEAHNLVDRAREM
                     FSAQMSKKSFLEMKNVMKDKNSKISRVLGKLNTFMLKMKRLCDDKDYYVSTAEQKDLY
                     PLLRNLISECDKYLAKDAKHGEDNEEFLQLYFDALAFLRISEFYDERYVAFIEKTEKD
                     VTIRLFCIDPSRLLSEALKRGKAAVFFSATLMPLDYFREILGGSENDYIMYLNSPFDT
                     NNRCLLVADGISTRYRDRDQSVKEVVECIKAVVQGKKGNYIAFFPSYSYMNSVYDILR
                     NEGLDIKIYIQSGSMSEKDREDFMDLFKEDPEETVLGFCVLGGIFSEGIDLKNDRLIG
                     AVIVGVGLPQICPERDIIRDYFENVKGAGFEYSYMYPGMNKVMQAAGRVIRSEKDRGI
                     ILLIDDRFTKQSY"
BASE COUNT          765 a          387 c          601 g          701 t
ORIGIN      
        1 attgaatatt ctaaagggta tggacaggag ccttgccggt ccgactgtcc cgcccaacgg
       61 acttgtttta tatgaggtgg agtactgaag aaatagactt aatgggattt tgttttaggt
      121 gcaggttttg cttggcggaa ttttatattc aacaagctta acctgcctgt gtgttaataa
      181 aaaggttgtg ttcaaatgag tgatgataaa aaagagttaa aggtgtccat caggaatctt
      241 gttgaatttg tgctccgctg cggtgatatt gacagctctt ttggaggaac caaccgggcg
      301 gtcgaaggta caaggcttca taaaaagatt cagaaggctc aaggcgaaga atacagtgcc
      361 gaagtaaccc ttaaaactgc agtggagttt gagggttttt ctcttgtggt tgaaggacgg
      421 gctgacggta taataaatga aaaaggctgt tttaccgttg acgagataaa gaccactacc
      481 atgccccttg attctataga tgaggacttt aacccaatcc actgggcaca ggccaaatgc
      541 tatgcttaca tccatgcttt aaacgagaat ttaaatgaga taaatgtaag actgacctat
      601 tgtcatttag acacggagga aataaaatat ctgaccaaaa cctttgattt tgcggagctt
      661 aaaaagtttt tttatgaact gattgaaaag tattatatct gggcgaaatt tgcctatgat
      721 tggcagatta ataggaatga ctccattaaa gctctgaatt ttccctttgc ccgttacaga
      781 aaaggccaaa gagaactggc ggtagctgtt tacaaaacca ttgaaggtgg taaaaaatta
      841 tttgtaaaag cacctaccgg tataggtaag actatttcta ctttgtttcc atcggttaaa
      901 attttgggag aagggcatat ctcaaaaatc ttttatctga cagcaaagac cattacccgg
      961 agtgttgccc aggaggcttt gtcaataatg agggagaagg gacttctaat aaaaaacgta
     1021 accctcactg cgaaagaaaa aatttgtttt atggaaaaat ccaattgcaa gcctgaaaag
     1081 tgcgaatatg caaaggggca ttatgacagg ataaatgatg caataatgga tgtgttgcaa
     1141 gaagagtgtg aaataaacag agaggttatt gaggaatatg caaaacgcca tagagtgtgt
     1201 ccctttgaaa tgtctttgga tttaaccctc tgggctgatg tgataatatg tgattataac
     1261 tatgcctttg accccagggt ttacctgaaa cgtttttttt cagacgccgg cggagattat
     1321 gcttttttgg ttgacgaagc tcataacctg gtggacagag cccgcgagat gttttcggct
     1381 cagatgtcta aaaagagctt tcttgaaatg aaaaacgtca tgaaagacaa aaattcaaaa
     1441 atctcccgag tgctgggtaa acttaatacc tttatgctga aaatgaaaag gctttgcgac
     1501 gataaggact attatgtcag taccgcggag caaaaggatt tatatccgct tttgagaaat
     1561 ctaatcagtg aatgtgacaa atacctggca aaagatgcaa agcacggtga ggataacgaa
     1621 gaattcttgc agctttattt tgatgccttg gcctttttga ggatatcgga gttttacgat
     1681 gagaggtatg tagcttttat tgaaaagaca gaaaaagatg tcacgataag gcttttttgc
     1741 attgatcctt caaggctttt gagtgaggct ttaaagaggg gaaaggcggc ggttttcttt
     1801 tctgctacgc tgatgccgct ggattatttc agagaaattt tgggaggaag cgagaatgac
     1861 tatataatgt atttaaactc tccttttgat acaaacaacc ggtgtcttct ggtagcagat
     1921 gggatatcca cccggtacag ggacagggat caaagcgtca aggaggtagt ggaatgtatt
     1981 aaagctgtgg tacagggcaa aaaggggaat tatattgctt tctttccttc ttacagctat
     2041 atgaattcgg tttatgatat attaaggaac gagggcttag atataaaaat ctatatccaa
     2101 tccggttcta tgtcggaaaa ggaccgggag gatttcatgg atttatttaa agaagatcct
     2161 gaagaaacgg tgttgggttt ttgtgttttg ggcggtatat tttccgaagg gattgacctt
     2221 aaaaacgaca ggttgatagg agcagtaatt gtgggggtag ggttgccgca aatatgccct
     2281 gagagggata ttataaggga ctacttcgag aatgttaaag gagccggatt tgaatactct
     2341 tacatgtatc cgggaatgaa taaggttatg caggctgcag gaagggttat acgctcggaa
     2401 aaggaccggg ggattatact gcttatagat gacagattta caaaacaaag ctac
//
LOCUS       DAOOJC010000370         2451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_33609_length_2451_cov_2.997913, whole genome shotgun sequence.
ACCESSION   DAOOJC010000370 DAOOJC010000000
VERSION     DAOOJC010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33609_length_2451_cov_2.997913"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="PLA01_09075"
     CDS             <1..1144
                     /locus_tag="PLA01_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPD01484.1"
                     /translation="TLDGKKVGMTSTGGVYIGDECILRGDNTGPVSASMVLASKETEA
                     AVLETARGGLVRKGLGYDLADVGVITNIAEDHIGVDGINTLEELVFVKSLVVEAVKPE
                     GYAVLNADDRFVDYFADRAKGDIILFSRSRANPVLLNHIKAGKKAIYTDNGIIYIFNG
                     KNAVPFMHVGEIPITFGGIVECNIENSLAAVSALYALNINLDVIKNGLAAFKPDREFN
                     PGRFNIIDMGSFKVMLDYSHNPAGYREVVKFVEKVEAKRLVGVIGVPGDRDNSSIYKV
                     GEISGRHFEKIYIKEDNDLRGRAAGEVANILYDAVIKSGIRKDGVEIVYSEDKAVEKA
                     ILDAKPGDFIVVFYEDFELASEVVERIASELSHSTVAAETVIQNVG"
     gene            1318..1881
                     /gene="folE"
                     /locus_tag="PLA01_09080"
     CDS             1318..1881
                     /gene="folE"
                     /locus_tag="PLA01_09080"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPD01485.1"
                     /translation="MIDKERIKAAVREILIGIGEDPDREGVIETPDRVARMCEEIFAG
                     LHQDPRSVVKVFQEENHEEMVLVKDIPIYSVCEHHLLPFVGVAHVVYIPKKGKIMGLS
                     KLARIVDLIAKKPQLQERLSSEVANVIMETIDPLGVAVVVEAEHLCMTMRGIKKPGSK
                     TVTSALRGIIKTDARTRSEVMALINGR"
     gene            complement(2158..2385)
                     /locus_tag="PLA01_09085"
     CDS             complement(2158..2385)
                     /locus_tag="PLA01_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPD01486.1"
                     /translation="MAIIIRLDRVLADRKMQLNELAEKVGISNVNLSNLKTGKVKAIR
                     FSTLNSICKVLSCQPGDILEYSDTDEDLYEE"
BASE COUNT          706 a          421 c          652 g          672 t
ORIGIN      
        1 aacccttgac ggtaaaaaag tggggatgac atcaaccggt ggggtgtata tcggggatga
       61 gtgcatattg aggggagaca ataccggacc ggtaagtgct tcgatggttc tggcttcaaa
      121 agaaaccgag gcagcagtac tggaaactgc gaggggcgga cttgtgagaa agggtcttgg
      181 ctatgacctt gcggatgttg gtgttataac aaacatagcc gaggaccata tcggagttga
      241 tggaattaat acccttgagg aattggtgtt tgttaagtca ctggttgttg aagcggtcaa
      301 gcctgaaggg tatgcggtat taaatgcgga tgacaggttc gtggattact ttgcggatag
      361 ggcaaaaggt gatattatac ttttttcccg cagcagggca aatcctgtgc tgttaaatca
      421 tataaaagcc ggtaagaagg ctatatacac ggataacggc attatttata tattcaacgg
      481 aaagaatgct gtgcctttta tgcatgtcgg ggaaattccc ataacctttg gaggaattgt
      541 ggagtgcaac attgaaaact cgctggcggc agtttcagcc ctttatgcac tgaatatcaa
      601 ccttgatgtc ataaaaaatg gacttgctgc cttcaagccc gatagggaat tcaatcccgg
      661 caggtttaac ataattgaca tgggcagttt taaagttatg cttgattaca gccataaccc
      721 tgcagggtac cgggaggttg tgaagtttgt tgaaaaagtg gaagcaaaaa ggcttgtagg
      781 agttatagga gtgccgggag accgcgacaa ttcaagcata tacaaggttg gagagatcag
      841 tggcagacat ttcgaaaaaa tatatataaa ggaagataat gatttgaggg gcagagcagc
      901 tggagaagtg gcgaatatat tatacgatgc agttattaaa agcggtattc gaaaggacgg
      961 cgtggagata gtgtattctg aggataaagc cgttgaaaag gcaatcctgg atgcaaagcc
     1021 cggggatttt atcgttgtgt tttatgaaga tttcgagctt gcctcagagg tggttgagag
     1081 gatagcaagc gagctttctc atagtacagt ggctgctgaa actgtaattc agaatgtggg
     1141 atagagggga ttgtttttgc cgaaagaatg tttttcaaaa gctctttgtc tttttatatt
     1201 aatggagtat aatgtataag tagctgacag ccatttgggt gtcaaagttt taagagaata
     1261 caggatgttt tatataagct gacccgaaga gatttttgaa tagaaagagg tattgctatg
     1321 atagataaag agcggattaa ggcggctgtg agggagatac tgatcggaat aggagaagac
     1381 cctgaccgtg aaggagtaat agaaactcct gacagagtgg ccaggatgtg tgaggaaata
     1441 tttgcaggac ttcatcagga ccccaggtca gtggtgaagg tgtttcagga agagaatcat
     1501 gaggaaatgg tgttggtaaa ggacattccc atatattcgg tttgtgaaca ccatttgctt
     1561 ccgtttgttg gggtagccca tgtggtttat atacccaaga aggggaagat tatggggctc
     1621 agcaagcttg cgaggatagt tgaccttatt gccaagaagc ctcagcttca ggaacgtctc
     1681 agcagtgagg ttgccaatgt tattatggaa accatagatc ctttaggagt tgctgtggtg
     1741 gtagaagccg agcatttatg catgacaatg aggggtataa aaaagcccgg ttccaagacg
     1801 gtgacttctg cattgagagg tataatcaag accgatgcca gaacccggtc cgaggttatg
     1861 gccctcatca acggccgcta acacaggggg acgtacccct tgtgtttgta taaaactgta
     1921 aagtaagctc ccaaaaacgt actttatcag tggtttcgaa ccgtcccccg tgctagtctg
     1981 tcacgggggg acggttcttc cgtgttattt attagtggga tttcacgaag tgaaatgacc
     2041 aaaaagtgat gggggttcga tacccccgga acttttattg agattgagtt tttaatcgac
     2101 acttcagtgg aggtggaatt ctccactgaa gcttcaatga ttgactaggc aaccggttta
     2161 ttcttcatat aaatcttcat ccgtgtcaga atactcaaga atgtctccag gctgacagct
     2221 taagaccttg cagatggagt tcagggtgga aaaccttata gcttttactt tgccggtttt
     2281 taaatttgaa agattaacgt tcgatattcc gactttttcc gccagctcat taagctgcat
     2341 ttttctatct gccaataccc tgtcaagacg aatgataata gccattatat tcaccgcctt
     2401 gtacaattaa tattgtatta aatcagtgac cgaagccact gttcgaccca t
//
LOCUS       DAOOJC010000371         2446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_33698_length_2446_cov_2.782518, whole genome shotgun sequence.
ACCESSION   DAOOJC010000371 DAOOJC010000000
VERSION     DAOOJC010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33698_length_2446_cov_2.782518"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLA01_09090"
     CDS             complement(<1..353)
                     /locus_tag="PLA01_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01487.1"
                     /translation="MSLGNNIALNIYSIAILLIIYFHALKFFEKDSMSDKLYMWILHI
                     TTLMLGIDILGRFDGNTSAIYPVFNHIGNFLIFLLNPVLPSLWVAYAHFQVFHDERRI
                     KRLFYPLCAINVINV"
     gene            complement(657..911)
                     /locus_tag="PLA01_09095"
                     /pseudo
     CDS             complement(657..911)
                     /locus_tag="PLA01_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075297.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
     gene            complement(936..1706)
                     /gene="ygiD"
                     /locus_tag="PLA01_09100"
     CDS             complement(936..1706)
                     /gene="ygiD"
                     /locus_tag="PLA01_09100"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HPD01488.1"
                     /translation="MSKMPVLFVGHGSPMNAIEDNNYSRNWRNLAERIPKPGTILSVS
                     AHWYTHGTRVMNEENPKTIYDMYGFPEELYEVLYDPAGSPGIAKAAKELISRETKYDN
                     SWGIDHGTWSVLVHMYPDRDIPVFQISIDASAPPEEHYKIGKELGALREQGVLIFGSG
                     NVVHNLRLVDWHKANKGFDWAYEFDEYIYENIMDYNHDNILKYNELGDIARLAVPIPD
                     HFYPLLYTLGASDEDDKVSVHNKSCELGSLTMTAYLWE"
     gene            complement(1830..2342)
                     /locus_tag="PLA01_09105"
     CDS             complement(1830..2342)
                     /locus_tag="PLA01_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HPD01489.1"
                     /translation="MLNQEVAKLLNEQINKEFYSAYLYLDMANYYADKNLAGFENWFF
                     VQAQEERDHAMLFRQYLLNNGESVISTEIAAPDEKYNNYKEPLEKAFEHEKYVTASIN
                     KIYEAAFKISDFRTMQFLDWFIKEQGEEEKNADDNIKKYELFGGDPKGLYMLDNEMKA
                     RVYAAPSLVL"
BASE COUNT          728 a          514 c          405 g          799 t
ORIGIN      
        1 atgacattaa ttacattaat ggcacataga ggataaaaca atcgtttgat tcttctttca
       61 tcatgaaata cctgaaaatg agcatatgcc acccacaatg atggtaagac cgggttcaga
      121 agaaaaatca ggaaattccc gatatgattg aatacaggat aaatagcaga ggtatttccg
      181 tcaaatctgc ctaatatatc tatgccaagc atcaaagtag tgatatgcaa tatccacata
      241 taaagcttgt ccgacataga atccttttca aagaatttta atgcatgaaa atagataatt
      301 agaagtatcg caatagaata tatgttaagt gctatattat ttcccagact cattaatgtt
      361 cactccgtgt taaggtcacc taaggcaact atttctgaaa atcatacaat ataatcaatc
      421 atatccatcg tacgttataa agcttatgga aattgagtgt accatataat atagtacgac
      481 aggcacctgt ccgtgtcaac ttatttatat ctcttggagg tggtcagatt ttttattcat
      541 ccaagctctg acaatcattt tccagttcag cagtttctgc caaagggtat attttaaaaa
      601 cagatgcact gcaaaaagca gagaatctgg gtatggcttt atgaccaatc ttttcgttaa
      661 tgatgatttc cgttatcgta acaccatttc gctatttcgg ctataagttg gagcggcagc
      721 ggcttgttga acggaaactg caccgcgcct ttgcttgttt tgtattccgt aagtctttcg
      781 ctgaaatgct caacagcctt atcgccgggg tacagcccga tgtgcctttt gaatgccgca
      841 aagtggataa tattccgttt acgccagtaa gtcggcatac tccatgagat acgctcttcc
      901 gcatatggga gtatttgcca cccactccac atgggttatt cccataggta agcggtcatt
      961 gttaaagacc ccagttcaca ggatttatta tgaacactga ctttatcatc ttcatccgaa
     1021 gccccgagtg tatatagaag aggataaaaa tggtcaggaa ttggtactgc cagccttgca
     1081 atatcaccaa gttcattata tttcaaaatg ttatcatgat tatagtccat aatattttca
     1141 taaatatatt catcaaactc atatgcccag tcaaagccct tgtttgcttt atgccaatca
     1201 accagtctca agttatgaac aacatttccg ctgccgaata taagaacccc ttgttccctt
     1261 aatgctccaa gctccttacc tattttgtag tgttcttcag gaggagcact cgcatctata
     1321 ctgatttgaa atacaggaat atccctatcc ggatacatat gaaccaatac cgaccaggtt
     1381 ccatgatcaa tgccccatga gttatcatat ttcgtttccc ttgaaatcag ctccttagcg
     1441 gctttagcaa tgccgggtga acctgccggg tcgtataaaa cttcatataa ttcctccggg
     1501 aaaccgtaca tgtcatatat tgtttttgga ttttcttcgt tcataactcg ggttccatgt
     1561 gtataccaat gggccgaaac agacaatatc gtacctggtt ttggtatcct ttcggctaaa
     1621 ttcctccagt ttcttgagta attattatcc tcaattgcat tcatcggtga cccatgtcct
     1681 acaaataaaa ccggcatttt cgacatagta cctgccatcc ctttcttaaa tcaatattca
     1741 ataattgtac ctgcctaagc gataatgcag caatttaaaa aaatatccga cgtcagctga
     1801 taagcagaga cgtcggaatt catatcatat tagaggacta gagatggtgc ggcatatacc
     1861 cttgctttca tttcattgtc taacatatac aatcctttgg gatcaccgcc aaacaattca
     1921 tatttcttga tgttatcgtc tgcattcttt tcttcctcgc cttgttcttt aatgaaccag
     1981 tcaaggaact gcattgtacg gaaatcacta attttaaatg cagcttcata tatcttgttg
     2041 atagaggcag ttacgtactt ttcatgttca aaagctttct ctaatggttc tttataatta
     2101 ttatatttct catcaggagc agcaatttct gtcgagatta ctgattcgcc attatttaat
     2161 aaatattgac ggaacaacat agcgtgatcc cgttcttcct gtgcctgtac gaaaaaccaa
     2221 ttctcaaaac cggccaaatt cttgtctgca taataatttg ccatgtccaa atataagtat
     2281 gcggaataaa actctttatt aatctgttca ttcaatagtt ttgctacttc ttgattaagc
     2341 atactacccc atcctttctt aatcttattg tttaatctta atagtatatt ccgtaagtat
     2401 ttttatacag gtgttcattt ccaattatat atcattatac aagtat
//
LOCUS       DAOOJC010000372         2442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_33772_length_2442_cov_3.755341, whole genome shotgun sequence.
ACCESSION   DAOOJC010000372 DAOOJC010000000
VERSION     DAOOJC010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33772_length_2442_cov_3.755341"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /gene="pheA"
                     /locus_tag="PLA01_09110"
     CDS             complement(<1..804)
                     /gene="pheA"
                     /locus_tag="PLA01_09110"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HPD01490.1"
                     /translation="MIKTGFLGPKGTFSQEAMIVYTKGNSEYSGVDYSTMTDLITAVS
                     KGEIDEAVVPIENSLEGAVSTTMDMLATEPDIKIKAEVIIPISQNLLIKKGTRIENIK
                     YILSHPQAVGQCRKYIDSNFPGAVIKYLYSTAEAAKEVAEGMADMAAVGSKAAAAVYD
                     LEILRESIQDNNNNLTRFIVISKKEAHKSIKNKTSIVFSTENKPGSLYRILDIFSLWD
                     INMTRIESRPAKNKLGRYIFFVDIEGHAEDEDVRDALTMVKRKTSFYKFL"
     gene            1057..2139
                     /locus_tag="PLA01_09115"
     CDS             1057..2139
                     /locus_tag="PLA01_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPD01491.1"
                     /translation="MWYNHKFFKFGSAVLLILLIIFMLGQIDFFLEPFRKLIASIFTP
                     LLISTLLYYVLRPLVNLLEKIRIRRTFGILLSFAAVLVLFIVSSTYTGTVIVEQFNQL
                     LKDLPTFDITWEKTLSFLNSDWLESLPLDKFQDKLIDILNSVPENLKTIFLGIVSTVT
                     NIGTMVFMIPFILFYFLKDDKYFLSNVVSHIPKKYRDNADKIIKDVDTTLSSYITGQM
                     LIALFVGIMMFVGYLIIGMKYSLLLGIFAMISSLIPFFGPTIGIIPALLLSLATNDLL
                     MFVKVIVVMVVVQQIDNNFISPYIMGQRLNVHPVTVILLITVGASLYGFLGLFLVVPI
                     YAAAKVSAKHFYEIYRKYKLKEVTAK"
BASE COUNT          691 a          482 c          413 g          856 t
ORIGIN      
        1 caaaaactta taaaacgatg tctttctttt aaccattgta agggcatccc tgacgtcttc
       61 atcctccgca tgtccttcta tatcaacaaa aaatatatac cgccccagct tattctttgc
      121 cggtcttgac tctattcgag tcatatttat gtcccacaga ctgaaaatgt ccaatatcct
      181 gtaaagactg cccggcttgt tttctgtgga aaaaactatc gatgttttat tcttgatgct
      241 tttgtgtgcc tcttttttgg atataacaat aaacctggtg agattattgt tgttatcctg
      301 tatggactcc cgaagaattt ccaggtcata tacggcggca gccgcctttg aaccgacagc
      361 agccatatct gccattccct ctgccacttc ctttgcagcc tccgcagtac tgtacagata
      421 ctttatcact gcaccgggaa aattggaatc aatgtatttc ctgcactgtc cgactgcttg
      481 cgggtgggag aggatatact taatgttttc aattcgtgtt ccctttttta ttaaaaggtt
      541 ctggcttatc ggaataataa cctctgcctt gattttaatg tcgggttccg tagccagcat
      601 atccattgta gtgcttacag caccctccag agaattctct atcggtacca ctgcttcgtc
      661 aatctcaccc tttgaaactg ccgtaatcaa atccgtcatg gtactgtaat caacgcctga
      721 gtattcactg ttcccttttg tataaactat cattgcctcc tgggaaaacg ttcccttggg
      781 ccctaaaaat ccggttttta tcataaagca ctcctcacga tattaaaagc tagaaaaaca
      841 ttattataca ttataataac atcaatctat gctttttcca actattatcg aggagacagg
      901 gtttatattg tttgtgaagc aactaccgaa gacatattgt caagcaagtc gttgccgcct
      961 gttattttct tcgtataatt ttttaataaa atctatcttt ttattaaata atatggtaag
     1021 tttatatatt atataactta ataagaggtg cgatatatgt ggtacaatca taaatttttt
     1081 aaattcggtt cggctgttct tcttattctt ttaattattt ttatgctcgg acagattgat
     1141 ttctttcttg agcctttccg aaagctgatc gcatcaattt ttacacctct tttaatatct
     1201 accctgctgt attatgtttt aaggccactt gtaaatctac ttgaaaaaat caggatcaga
     1261 agaacctttg gaatacttct aagttttgca gcagtattgg ttttattcat cgtttcgagc
     1321 acctataccg gcactgtcat agttgaacag ttcaatcagc tcttaaaaga ccttccaacc
     1381 tttgatatta catgggaaaa aactttgagc tttttaaaca gtgattggct tgagtctctg
     1441 cctcttgata aatttcagga taaattaatc gatatattga actccgttcc cgagaatctg
     1501 aaaacgatat ttctcggaat agtatccaca gttacaaata tcggaaccat ggtttttatg
     1561 attcccttta tacttttcta ttttcttaag gacgataagt actttttatc caatgttgtc
     1621 tcccatatcc cgaaaaaata ccgggataat gccgacaaaa taataaagga tgtggacaca
     1681 acactttcct cctatattac aggacagatg ctcatcgcac tgtttgtcgg tataatgatg
     1741 ttcgttggtt accttataat aggaatgaaa tactccctgc tgttggggat atttgccatg
     1801 ataagctcgt taattccatt tttcgggccg actataggga ttattcctgc actgctctta
     1861 agccttgcta ccaacgattt gttaatgttc gtcaaggtaa tcgttgtgat ggttgtcgtt
     1921 cagcagatcg acaataattt tatttcccca tatattatgg gtcaaaggct taatgttcac
     1981 ccagtaacgg ttatactcct cattaccgtc ggagcatccc tttacgggtt cttagggctt
     2041 tttctcgtgg tacccattta tgctgcagca aaggtttcgg caaagcattt ttacgagata
     2101 tacagaaaat ataaactaaa agaagttact gcaaagtaaa atatttatat ataacgtaat
     2161 ttacaggcgt cttgatatat attttttatc atgctattat ttataatggg cttttttacg
     2221 atatagaaaa agttcataac tataattcat tactctatgt attaatttaa gagcttgggt
     2281 ttgtgccttt acggcattta cagcatacca ttatcagcct tgcacccggg ttttatgaag
     2341 tgttttagta atagatactg ttttaaataa cataaacagc tttagattgg aaattaatat
     2401 acaaggtggt gataaagtga caatagattt aattaagagc at
//
LOCUS       DAOOJC010000373         2437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_33874_length_2437_cov_4.048279, whole genome shotgun sequence.
ACCESSION   DAOOJC010000373 DAOOJC010000000
VERSION     DAOOJC010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33874_length_2437_cov_4.048279"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..618)
                     /locus_tag="PLA01_09120"
     CDS             complement(223..618)
                     /locus_tag="PLA01_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HPD01492.1"
                     /translation="MIVFGWGYRTVKNYGPVRKIQCSNCSNETSWLLQKVTTWLTLFF
                     IPVIPYKRNNILVCPVCRCCLELSKNEFEELKSIIQDAKFFESDEARRHMNNIVTEAG
                     GIIRTETQLNFIRDMQEFEREKEKKNKLT"
     gene            complement(837..>2437)
                     /locus_tag="PLA01_09125"
     CDS             complement(837..>2437)
                     /locus_tag="PLA01_09125"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPD01493.1"
                     /translation="IFNPVNAKTQSRIDSLSAPLSDRVLLVVEADEALYLKVRAWDKY
                     ENNSWKSGNRELAEYQPIEDIYWSQVKYDTLVSLLKRAKEEGGIPPLLSEHNELWDFD
                     SVKRAKRTAVLTNASQVFVNSLPIPVGILDADCKNRDEYINRYGMCAVRTFPLTYPWD
                     FYRIEYISSRLTLNSQEYKLMRILNKTIAEKLLNPASYRTDEGNSARFTLGYDELTVL
                     GVAAAELNHTYENYMQLPQNISQRIYKLAESITEGKDSDYDKAKAIEQYFHTSDFVYD
                     LNPPRIPDGAEANDYFLFESRSGFCIHYASAMVVLARACGLPARYAEGYVADEFEYET
                     GRYIVREKDAHAFPEVYIAGYGWMVFEPTVSNMAKSKWDLIIDRLRVTVEFLGTTISK
                     VIDITPVWVRILFIPFVIFTILFTVWVLRRLYIQAWIGKNLIMERDKAVYRIFDRIIK
                     LLGVANLGMIKGETASQYERRIYNKTGLDLSDFIQIFNKSKYAGIKPSIDDVRVGITA
                     YKDVVVYVKGRMGRFNFLKFFWFV"
BASE COUNT          764 a          547 c          340 g          786 t
ORIGIN      
        1 agcaaaatga agcatgttta aaattttgat gtcctattct aattgacgct tacatttaac
       61 aaagcattat attgcacaaa aaaaccctag catcatttca tcctaatact taattagaat
      121 tactttgtgc tgggttcttg gccgcttgct gtacaactct tgttgatcct attcactcat
      181 ttcattttag ctatattttc caaaatgtca tatacaacaa atttatgtca atttattctt
      241 cttttccttc tctctttcaa attcctgcat atccctgata aagttcaact gagtctccgt
      301 tcgaataatt cctcctgctt ctgtcacaat attgttcata tgtcttcttg cctcatcgga
      361 ctcaaaaaac tttgcatctt gaattatgct ttttagttct tcaaactcgt tttttgaaag
      421 ttccaaacaa cacctgcata ccggacaaac cagtatatta ttcctcttgt aaggaataac
      481 cggaataaaa aacagagtca accaggttgt aaccttctgc aaaagccaac ttgtttcatt
      541 actacagttg ctgcattgta ttttacgaac cggtccatag ttttttacgg ttctgtagcc
      601 ccatccaaat acaatcataa taaaacctcc tgtagtttgg ttaactgaaa taaactcatt
      661 cgaaacaaat agactgaact aattttgcaa ataaaaacaa atggagtaaa aacaaggtgt
      721 tgtataagaa atgaaaataa ataaaacgca tttaaacctt acacccatat tttactccat
      781 tttattacga ttttcaagta ctattttatt attattttac ataaagtatt atttctttaa
      841 acaaaccaga aaaacttcaa aaagttgaat ctccccatac gaccttttac gtatacaaca
      901 acatctttat atgcagtaat tccaaccctc acatcatcta ttgaaggttt aattccggca
      961 tattttgatt tgttgaatat ctgtataaaa tccgacaggt ccagcccggt tttattataa
     1021 atcctccttt cgtattgtga agctgtctca cccttgatca ttccaagatt cgcaacgcct
     1081 aaaagcttta taattctgtc gaaaattcta taaacagcct tgtctctctc cattataaga
     1141 ttttttccaa tccatgcctg aatataaagc cttcgcaaaa cccaaacagt aaacagaata
     1201 gtaaaaataa cgaagggtat aaagagaatt ctaacccaaa ccggagtaat atcaatgacc
     1261 tttgatatag ttgttcccaa aaattcaact gtcacccgaa gcctatcaat aattaaatcc
     1321 cacttgcttt tggccatatt actcactgtt ggctcaaata ccatccatcc gtatcctgct
     1381 atataaacct caggaaatgc atgggcgtct ttttcacgaa caatatatct cccggtttca
     1441 tattcaaatt catcagcaac atatccctct gcatatcttg caggcaaacc gcatgccctt
     1501 gccaacacca ccatggcaga ggcataatga atgcaaaatc cgctcctgct ttcaaaaaga
     1561 aaatagtcat tggcttctgc accatccgga atgcggggag gatttaaatc atacacaaaa
     1621 tccgatgtgt ggaaatactg ttctatagct ttggccttat cataatcact atctttccct
     1681 tcagtaatac tttcagccaa tttataaatc ctttgggaaa tattttgagg caattgcata
     1741 tagttttcat atgtgtggtt taactcagcc gctgccacac ccaacacagt taattcatca
     1801 tagcctaatg taaatctcgc actgttaccc tcatccgtcc tataggatgc agggttcagc
     1861 agtttctccg ctattgtttt atttagaatt ctcataagct tgtattcctg tgagttcaaa
     1921 gttaatctgg aggatatgta ttcaatgcgg taaaagtccc atggatatgt caaaggaaac
     1981 gtacgtaccg cacacattcc atatcggttt atgtattcat cgcggttctt acaatcagca
     2041 tctaaaatac caacaggtat cggaagcgaa ttcacaaaaa cctgacttgc attagttaaa
     2101 actgcagttc tcttagccct tttgaccgaa tcaaaatccc acaattcatt gtgttcagac
     2161 agcaacggag gaatccctcc ctcttccttt gctcttttta acaatgaaac aagagtgtca
     2221 tatttaacct gagaccaata aatatcttca ataggttggt attctgccaa ttcacgattt
     2281 cctgacttcc atgaattatt ctcatatttg tcccaagccc gcacttttaa gtacagtgct
     2341 tcatcagctt ccacaactaa gagcaccctg tcactcaaag gtgcactcaa agaatctatc
     2401 ctgctctggg tcttggcatt cacaggatta aaaatat
//
LOCUS       DAOOJC010000374         2431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_33989_length_2431_cov_4.276094, whole genome shotgun sequence.
ACCESSION   DAOOJC010000374 DAOOJC010000000
VERSION     DAOOJC010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33989_length_2431_cov_4.276094"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PLA01_09130"
     CDS             complement(<1..486)
                     /locus_tag="PLA01_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPD01494.1"
                     /translation="MNNRITETGANKVFFVVVVLNLVYFLGIKVVDRFCFGFLRANNY
                     LILIINQFYFILFPVLLYLVVKKIDIKEALKINKFRVLPGVLILLISIPAYMGASSLN
                     VLIVYILQSTGYALGESLPAPQSFGDIILAILVVAVSPAICEEILHRGVLLKAYESRG
                     SI"
     gene            complement(499..1086)
                     /locus_tag="PLA01_09135"
     CDS             complement(499..1086)
                     /locus_tag="PLA01_09135"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPD01495.1"
                     /translation="MRSKKDIRKEILNKRIGLIKGDVINRSIRITDKLTSLERFKESK
                     IIMTYMDFKNEVMTSFLTEYCLKLGKTLAFPLIESFQGVKKICAYEPKDHINGLKLGA
                     YGILEPVRQLSKKIDPKEIDMVVVPGVAFDTKGYRIGYGAGYYDVFLKEVKDECFKVG
                     IAFELQVIENIPKEEHDVSLDAIVTESRVILIKDF"
     gene            complement(1114..1806)
                     /locus_tag="PLA01_09140"
     CDS             complement(1114..1806)
                     /locus_tag="PLA01_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01496.1"
                     /translation="MDGTNSFNRVCSFCGESIAADYKRCPYCGSLLGLDKTHSGADSR
                     DVSPGNENSIYKGARNILNEDDSIQKNTSNVPENEVNIPKKESNVWEGTSSIPKGPGY
                     VREDEGGFSEKAVPYKQNINYGTAAGYEIKQSAANQSVYKNNTIAHSKEAGTSDRVPS
                     MGNGVKVFLVTLSNLLPGIGQLIGVIAGIVFMNAEEDKDKKSFGRAILISSMVVFLIM
                     MILFGLLAAYFA"
     gene            complement(1873..2007)
                     /locus_tag="PLA01_09145"
     CDS             complement(1873..2007)
                     /locus_tag="PLA01_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01497.1"
                     /translation="MIALFRRTEGTGTESKNDCTRWNDAVTGGVFIGRMEGFFPVLQN
                     "
     gene            complement(2004..>2431)
                     /locus_tag="PLA01_09150"
     CDS             complement(2004..>2431)
                     /locus_tag="PLA01_09150"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HPD01498.1"
                     /translation="IATVKKAIEVNKANPDDPIDVLAKLGGFDIAGLAGCFIGAAAYR
                     IPILIDGFISSCAALLAVRMEPKVRAYVFSSHGSAEPGSRKVMKELGLDPILNLGMRV
                     GEGTGTALAFNLIDAAFEAYTKMGTYEDAEIDKYMSENR"
BASE COUNT          717 a          609 c          357 g          748 t
ORIGIN      
        1 tatagaacct ctgctttcat aggcctttag cagaacaccc cgatgcaata tttcttcgca
       61 tatagccggt gatactgcca caacaaggat tgccaaaata atatccccga agctttgcgg
      121 tgctggaagg gattctccca aagcataacc tgtagactgt aaaatgtaga ctataagaac
      181 attaagtgac gaagccccca tatacgcagg tatggaaatc aaaagaatca agactcccgg
      241 caaaacacga aatttattga ttttcaatgc ttcctttatg tctatctttt ttacaacgag
      301 gtataacagt accggaaaaa gtatgaaata aaactggttt ataataagta ttaaatagtt
      361 gtttgcccgc aaaaaaccaa aacagaatcg gtcaacaact ttaatcccca aaaaataaac
      421 taaattcagc actactacaa caaagaaaac tttgtttgct ccggtttccg ttatcctgtt
      481 attcacagct ttatcctctc aaaaatcttt tattagaata actctgcttt cggtaacaat
      541 tgcgtccaaa gaaacatcgt gctcttcctt tggtatgttt tcaataacct gtaattcaaa
      601 agcaattccc accttgaagc actcatcttt gacttctttt aaaaaaacat cataatagcc
      661 cgcaccataa ccaatcctat atcctttggt atcaaaagca actcccggta caaccaccat
      721 atcgatttct ttaggatcaa tttttttcga aagctgcctt acaggttcta agatcccata
      781 agcccccagt tttaagccat ttatatggtc cttcggctca taggcacata ttttctttac
      841 tccctgaaag gattcgatta aaggaaacgc caaggttttc ccaagcttca agcaatactc
      901 cgttaaaaaa cttgtcatga cctcattctt aaagtccata taagtcatta tgattttgct
      961 ttctttgaac ctctcaaggg aagtcagctt atctgtaatc cggatacttc tatttataac
     1021 atcgcccttt ataagcccta ttctcttatt caatatctct tttctgatgt cttttttact
     1081 cctcataccg tcacctgtat agccccttta ttattacgca aaataagctg ccaaaagacc
     1141 aaataatatc atcattatta aaaatactac catagaagat attaaaatag ccctgccgaa
     1201 agatttcttg tctttatcct cctcggcatt cataaatact ataccggcta ttactccaat
     1261 taattgtccg attcccggta aaagattcga taatgtaaca agaaaaacct taacaccgtt
     1321 gcccattgac ggaaccctgt ctgaagtgcc ggcttcctta ctgtgtgcta ttgtattgtt
     1381 cttgtaaaca ctttgattgg cggctgactg tttaatttca taaccggctg ccgtaccgta
     1441 atttatattc tgtttataag gcactgcctt ttcggaaaag cctccttcat cctcccggac
     1501 ataacctggg ccttttggaa tactgctcgt accttcccag acattgcttt ctttctttgg
     1561 aatattaacc tcattctccg gtacattact tgtattcttt tgaatactgt catcctcgtt
     1621 caaaatattt cttgcaccct tgtaaatact gttttcattg ccaggactca catccctcga
     1681 atctgctcct gaatgtgtct tgtccagtcc tagcaaactt ccgcaatacg gacaacgctt
     1741 ataatccgct gcaatacttt caccgcagaa actacagacc ctattgaaac tgttggtgcc
     1801 atccataatt tatcctcccc gtaacacagt gattatgcct tcatccaaag aacataacat
     1861 gtcttaatca tattaattct gcaataccgg gaaaaatcct tccatccttc ctataaaaac
     1921 accacccgta acagcatcat tccatcgagt acaatcgttc tttgattctg tcccggttcc
     1981 ttctgttctt cgaaataatg caatcatctg ttttcagaca tgtacttatc aatttcggca
     2041 tcctcataag tgcccatttt cgtgtaagcc tcaaaagccg catcaattag attgaaagca
     2101 agggcagtac ctgttccttc tccaaccctc atgccaagat tcagtatggg atccaacccc
     2161 agttccttca taactttcct gctcccgggt tctgccgagc catgggaaga aaaaacatat
     2221 gcccgaacct tcggttccat tctcacagcc aaaagagcag cacaggagga aataaaccca
     2281 tcaatcaata tgggaatcct atatgccgca gcaccaataa agcaacccgc caatccggct
     2341 atatcaaatc ctcccagttt cgccaataca tcaataggat cgtcgggatt ggctttgttt
     2401 acttctatcg ccttttttac cgttgcaatc t
//
LOCUS       DAOOJC010000375         2430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_34018_length_2430_cov_3.011368, whole genome shotgun sequence.
ACCESSION   DAOOJC010000375 DAOOJC010000000
VERSION     DAOOJC010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34018_length_2430_cov_3.011368"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..805)
                     /locus_tag="PLA01_09155"
     CDS             complement(65..805)
                     /locus_tag="PLA01_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HPD01499.1"
                     /translation="MVRFAAKSDKGKVRELNEDSFRVITGYSNVPDAFIVADGMGGHS
                     SGEVASGMAVEFAEEYILTHSEVFSLEESVLSGIKKLMEEANTAIFTKASESQSNFGM
                     GTTFIAVVMLNNKMYIGHVGDSRVYLIRDGVIEKVTTDHSFIEELIKNGSLTREEAEN
                     HPKKNVITRALGCDQEIQVDTLTVEVKDNDIFVLCTDGLTNMLKEDEILDIILKNEDP
                     EAACNELIHWANDKGGEDNTTVIIIKNS"
     gene            complement(913..1956)
                     /gene="rlmN"
                     /locus_tag="PLA01_09160"
     CDS             complement(913..1956)
                     /gene="rlmN"
                     /locus_tag="PLA01_09160"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPD01500.1"
                     /translation="MEEKLDLLNMTIEELEDLLVKTGYKKFHGKQIFQWTNKGIKDID
                     EMSNLSKELRNKLKEVAYINRLEIVKKLVSSIDGTVKYLFRLTDGSTIESVLMEYQHG
                     YSTCISSQVGCRMGCRFCASTGAGFVRNLTSGEMLDQIISIQADAKKRIGNVVIMGIG
                     EPLDNYDNVVKFLRLVNHKDGLNIGARHISVSTCGLVPKILQLSKESLPVTLSVSLHA
                     PNDSIRDKIMPVNKRYSIDKLIEACKIYTETTNRRITFEYALINGVNDSKDSALELAG
                     RLKGMLCHVNLIPVNTVDGAGFNRSTKEKTAGFKGILERCGIETTVRRELGSDINAAC
                     GQLRRGLVDEKGI"
     gene            complement(1961..>2430)
                     /locus_tag="PLA01_09165"
     CDS             complement(1961..>2430)
                     /locus_tag="PLA01_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HPD01501.1"
                     /translation="IDEAKNRLGIDIIKTEVFDATILDQKLVGKIDRVLVDAPCTGLG
                     IIRRKPDIKWSRNPGDKKEIAALQERILSVASKYVKKGGVLVYSTCTIEPEENEEMVE
                     RFIKENGDFYLDDLSGLVPQTLKKEGLNKGYLQLYPNVDGVDGFFIARMIRGS"
BASE COUNT          649 a          622 c          349 g          810 t
ORIGIN      
        1 ttcagcacct ctgcttctta atgcattaac aacttacatt tgactcctta gccctgtcat
       61 accgtcaact gttttttata ataataacag tggtattgtc ttctccaccc ttatcattgg
      121 cccaatgtat cagttcatta catgctgctt cagggtcttc gttttttaat attatatcca
      181 gtatttcatc ttcttttaac atgttggtta gtccatccgt acacaaaaca aagatatcat
      241 tatccttcac ttccacggta agggtatcca cctgaatttc ctggtcacac cccaaagccc
      301 ttgtaataac attcttcttc gggtggtttt cagcttcttc ccgtgtcaat gaaccgtttt
      361 ttatcaattc ctcaataaaa gagtggtccg tagttacctt ttcaattact ccgtctctta
      421 ttaaataaac tctgctgtct cctacatgac cgatatacat tttgttgttc agcattacaa
      481 cagcaataaa ggtagtaccc ataccaaaat tcgattgtga ctctgaagcc ttggtaaata
      541 ttgcagtgtt tgcctcttcc atcaactttt taatgcctga caaaacactc tcttcaaggg
      601 aaaacacttc agaatgtgtt aatatgtact cctctgcaaa ctcaacagcc ataccgctcg
      661 caacttctcc ggaactgtgt ccgcccattc catcggcaac aataaaggca tcaggcacat
      721 tcgagtatcc tgttattacc ctgaaactgt cttcattaag ttctctaact ttccccttat
      781 cgctttttgc ggcaaatctc accataccac tccccaggtc agcaattaaa atgttttagt
      841 caaccaccac atgctgcaaa tatatattac taaaaaacca ttttaattca tttcatttat
      901 ttattataac atttaaatac ctttttcgtc caccagtcct cttcgaagct gtccgcatgc
      961 agcatttata tcacttccca gctcccgcct aaccgtagtt tcaataccgc acctttccaa
     1021 aattcctttg aatcctgcgg ttttttcttt tgtgcttcta ttaaatcctg caccgtcaac
     1081 ggtattcacc ggtataagat tgacatggca caacatgcct tttaaacgtc cagcaagctc
     1141 caaagcactg tctttcgaat cattcacacc gttaatcaaa gcgtattcaa aagttattct
     1201 tcgattagtg gtctcagtat atatcttaca tgcttcaatt aatttgtcaa tagaatacct
     1261 tttatttaca ggcataattt tgtcccttat actgtcatta ggtgcatgaa gggatacgga
     1321 taatgtaacc ggcaagcttt ccttggacag ctgaagaatt ttgggaacaa gaccgcatgt
     1381 tgaaaccgaa atatgccttg ctccgatatt caagccgtcc ttatgattga caagccttaa
     1441 aaactttaca acattatcgt aattgtccag gggctccccg atgcccatta ttaccacatt
     1501 gcctattctt ttcttcgcat ctgcctgaat ggatatgatc tggtccagca tctcccccga
     1561 tgtaagattc ctcacaaacc cggcaccggt tgaagcacaa aacctgcagc ccatcctgca
     1621 acctacctgt gaagatatgc atgtactgta accgtgctga tattccatca gcacgctttc
     1681 aattgtactt ccgtctgtaa gcctgaaaag gtactttaca gtaccatcaa tactcgaaac
     1741 aagtttctta acaatttcga gcctgtttat atatgctacc tctttaagct tatttctaag
     1801 ttcttttgag aggtttgaca tttcatcaat gtctttaata cccttgtttg tccattggaa
     1861 aatctgtttt ccgtggaact ttttatatcc cgtcttgacg agcaaatcct caagctcttc
     1921 tatcgtcata ttgagaaggt ccaatttctc ttccatcaaa ctaactccct cttatcattc
     1981 tggcaataaa aaacccgtca acaccgtcaa cattcggata caactgcaaa tatcctttat
     2041 taagaccctc ttttttcagt gtttgcggaa ccaaccctga taaatcatcc aaataaaaat
     2101 ccccgttttc tttaataaat ctttccacca tctcttcatt ctcttccggt tctatggtac
     2161 aggtgctgta aacaagcact ccaccctttt tcacatattt agaggccaca gatagaattc
     2221 tttcctgaag agctgctatc tctttcttat cccccggatt tctagaccat ttgatatccg
     2281 gcttccttct tattatgccc aacccggtgc acggagcatc caccagaacc ctgtcaatct
     2341 ttccgacaag tttctggtcc aggattgttg catcgaaaac ctctgttttg attatatcga
     2401 tacccagtct gttttttgct tcatcaatca
//
LOCUS       DAOOJC010000376         2405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_34520_length_2405_cov_3.466809, whole genome shotgun sequence.
ACCESSION   DAOOJC010000376 DAOOJC010000000
VERSION     DAOOJC010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34520_length_2405_cov_3.466809"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..108)
                     /locus_tag="PLA01_09170"
     tRNA            complement(37..108)
                     /locus_tag="PLA01_09170"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73..75),aa:Arg,seq:ccg)
     gene            complement(207..827)
                     /locus_tag="PLA01_09175"
     CDS             complement(207..827)
                     /locus_tag="PLA01_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01502.1"
                     /translation="MSNNSLLDILLFPKSFYKKISDKLLTLYLGIILVGFIDMGFGLY
                     TDIPMYFWSKPPTTLMFNISLTLCFVVLAGLIDIVFFALPLFDIFKFFRVKEKIKNIN
                     GQLIKLMKIYVVSHFLVVPVNAFFLFLSNGPYGENGSVSIISFFVLTVVTPIWHCAIV
                     TRGINAIYSFDDRLKNAVFPLVYVWTTLLGYALGFIINNWLIVFLM"
     gene            complement(994..1341)
                     /locus_tag="PLA01_09180"
     CDS             complement(994..1341)
                     /locus_tag="PLA01_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01503.1"
                     /translation="MVCDNTKRVVVIRNIPSNFIEEAILVLKSKPEGNAGKDIKKVFS
                     KNDKKISDHILIEAEEVINNYIKKNGAQRELGIELHLRPCHKKPKLLTSTVINLALLG
                     SIVLLLFMIAKLL"
     gene            complement(1517..>2405)
                     /locus_tag="PLA01_09185"
     CDS             complement(1517..>2405)
                     /locus_tag="PLA01_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPD01504.1"
                     /translation="ELENDGVKVRDILVYKNKWGKFEVNIFHQGCAGSNKCLDSVEKM
                     ASSVLGRRMVKDRSECMYNHKAGVCNLKLVEEEVFSITTGVARMAKHESRISGDNHTF
                     MNIGDGKHVMALSDGMGTGQRAFNQSKAAIGLLEQFMETGFDKDTAIRLINSILVLKS
                     DDDSFATIDLCAVDLYDGKVEFVKIGAVPTFIKRPRRIEVIRTVSLPAGILSTIETEL
                     VCKNVDNGDFIIMVSDGIIDSFKQEEGGEQTLIKFIEDIDSINPQAIADSILSEACSR
                     CGNKPVDDMTVLVAKVWKR"
BASE COUNT          754 a          561 c          384 g          706 t
ORIGIN      
        1 cagcatttgc atgaatactg gtgcttagga atttagcgcc ccggatagga atcgaaccca
       61 tatcagtgga accggaatcc accgtcttat ccattagact accgggacat aattaaattt
      121 tttggcaaga accgtctatg aaaaaatggt tcaccaaata ttataacaga atttaacggt
      181 ttgacaactg aaaattatcc tcaactctac attaaaaata caatgagcca gttattgata
      241 atgaatccca aggcatatcc cagcaaagtt gtccacacat acactaaagg aaacaccgca
      301 tttttaagcc ggtcatcaaa gctgtaaatc gcatttattc ctctggtcac gattgcacag
      361 tgccagatgg gagtgacaac cgtcaaaaca aaaaatgaaa taatggaaac agacccattt
      421 tccccgtaag gcccgtttga aaggaaaagg aaaaaagcat taaccggcac aacaagaaaa
      481 tgggacacca catatatctt cataagcttt atgagctgtc cgttgatatt ttttattttt
      541 tccttcaccc tgaaaaattt gaatatatca aacaggggca aagcaaaaaa aacaatatct
      601 ataagcccgg ccaatacaac aaaacacagc gtgagggaaa tattgaacat tagtgtggtc
      661 ggcggtttac tccaaaaata catcggtata tcggtgtata aaccgaaacc catatctata
      721 aaccccacca gtataatccc caggtacaga gttaataatt tatccgatat ctttttataa
      781 aatgactttg gaaaaagcaa tatatccaat aaactgttat ttgacacgat agattttccc
      841 cttaatcaat tctgattccc ctgtcatttg atttttttga aatctactac taataataca
      901 acctaattca ggtaaaagca atgaaaaaat tgaaaaaaag cccagtcaaa aaatactgcc
      961 atacaaaata cgggtaaaaa ttatcaatac atatcacagg agttttgcaa tcataaaaag
     1021 cagcaaaaca atactaccga gcaaagccag atttataacg gtgcttgtaa gcaacttcgg
     1081 ttttttatgg cagggtctta agtgaagctc aatacctagt tctctttgag ccccattctt
     1141 cttgatatag ttgtttatta cttcctccgc ctctattagg atatggtcgc tgattttttt
     1201 gtcattcttc gaaaaaacct tcttaatatc cttgcccgca tttccctccg gcttgctctt
     1261 taaaactaaa atagcctctt caataaaatt agaaggtata ttccttatta ctacaactct
     1321 tttagtgtta tcacatacca taagactcct ccgaataatc tgttattcta aaaagctttt
     1381 acatatcaga ctttttggaa tacatagtcc ctattgccgt cacagataat ttgctgctta
     1441 aacccaagta acgaccgcaa tatgtattca gagatagttt gtaccaatgc ccaggaaatt
     1501 atacacaaat ctttgtctac cgtttccaaa cctttgccac cagtacggtc atgtcatcca
     1561 caggtttgtt tccgcatctt gaacatgcct cggaaagtat gctgtcggca atagcctgag
     1621 gatttatgct gtcaatgtct tcgataaact taataagtgt ttgctctccc ccctcttcct
     1681 gcttaaagga gtcaataatc ccgtcactga ccattattat aaaatctcca ttgtccacat
     1741 ttttgcacac aagctcggtt tctatagtgc tcaatatgcc cgccggcagg gaaacagtcc
     1801 ttatgacttc aatcctgcgg ggtcttttta taaatgtggg aacagcccct atcttcacaa
     1861 actcgacttt cccgtcatat aggtccacag cacaaaggtc aatggtggca aaggaatcgt
     1921 catcggattt gagcacaagt attgaattaa taagccttat ggcagtatcc ttgtcaaaac
     1981 ccgtttccat aaactgctcc aaaagaccga tggctgcctt gctctggtta aaagccctct
     2041 gtccggtgcc cattccgtcg cttaaagcca taacatgctt accgtcacca atgttcataa
     2101 aggtatgatt atcaccggat atcctactct catgctttgc catcctggca actcctgtag
     2161 tgatgctgaa aacttcctcc tccaccagct tgagattgca cactcctgct ttgtggttgt
     2221 acatacattc acttctgtct tttaccatcc ttctcccaag gacggaagat gccatcttct
     2281 caacagaatc caagcattta ttggacccgg cacatccctg gtgaaaaatg ttaacttcga
     2341 attttcccca cttgttttta taaaccagaa tatctcttac cttcacaccg tcattttcca
     2401 gctcc
//
LOCUS       DAOOJC010000377         2399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_34660_length_2399_cov_3.279010, whole genome shotgun sequence.
ACCESSION   DAOOJC010000377 DAOOJC010000000
VERSION     DAOOJC010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34660_length_2399_cov_3.279010"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1868
                     /locus_tag="PLA01_09190"
     CDS             <1..1868
                     /locus_tag="PLA01_09190"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HPD01505.1"
                     /translation="KLVKAVSMRMGMEGMAERYLDLVALGTVADVVPLLGENRIIVKH
                     GLPMVENTTNIGLRALINVSGVEGKKASSFTMSFCLAPRINAAGRIGDAARAVMLFTT
                     RDEAEADKIASELEQENRLRQENELAILEEVKNSIEKNIDLKKDKVIVVAGEGWHHGI
                     IGIVASKITEKYYRPCIIISVEGDMAKGSGRSIEGFNLFEALEYCRDMLEKYGGHEQA
                     AGLTLKGDRIDDLRRRINEYADSVLDKSDLVRKVKVDANIKKEDIVLENVRMLELLEP
                     FGQSNPQPLLRYDNIVINGIRAVGQNKHAKIRFNDDGFIIEAIGFNMGEMAKVYAESD
                     VVDVVCSLEANLWNGNESVQLNIKDLRPGYKVIRENKFYLSLDKSVDFGFSKKDDAVD
                     EVLSGISKIDSRIDIAEAVNEFLSRNERVAILVNSINGLHSIESALERNLLAIRDKYM
                     TYYLYAKPEDSRPVSVIVNPYPVKEYFSNFDRVILYGEWTSKHYLYNIVRLIDLNRVY
                     VYNRIIITFNDDDILLKRQDIAAVFKYLKSNYDRDIIIENLFVLARGIERQFSIPMNY
                     FKVKRAIQVLEEISILTKMPFGKYGLRITMLDTGKEKKNLEDSCLFRSIRTLLSS"
     gene            1917..>2399
                     /locus_tag="PLA01_09195"
     CDS             1917..>2399
                     /locus_tag="PLA01_09195"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HPD01506.1"
                     /translation="MDLKSKLREVMNFPKEGINFIDITTVLQDAQALKQCIDAMKEKI
                     ETFGDFDLIVGPESRGFIFGTPLAYAMGKGFIPIRKKGKLPYQTINVEYQLEYGKDFL
                     EMHIDAVKEGQKVVIVDDLLATGGTTESNIKLVEQLGGEVVGIVYFVELSFLNGRDRL
                     K"
BASE COUNT          790 a          329 c          603 g          677 t
ORIGIN      
        1 ataagcttgt taaggctgtt tccatgcgta tgggtatgga ggggatggcg gagcggtatc
       61 ttgacctggt tgctctgggt actgttgcag atgtagtccc tcttcttggg gagaacagga
      121 ttattgtaaa gcatggtctt cctatggttg aaaacacaac taatattggg ttgcgtgcac
      181 ttataaatgt atccggagtg gagggaaaaa aggcttcttc ttttacgatg agcttttgtc
      241 ttgctccgag gataaatgca gcaggaagaa ttggtgatgc ggcaagggca gtaatgctgt
      301 ttaccacaag ggatgaagcg gaagctgaca agatagcatc ggaacttgaa caagagaaca
      361 gattacgcca ggaaaatgaa ctcgccatct tggaagaagt gaaaaacagt attgaaaaaa
      421 atatagactt aaagaaggat aaggtaattg ttgtggcagg agaagggtgg catcatggca
      481 ttataggcat tgtagcttca aaaattaccg aaaaatacta tagaccctgt ataattattt
      541 ctgttgaggg tgacatggca aaaggctcgg gaagaagtat tgaagggttt aacctgtttg
      601 aggcattgga atattgccgg gatatgctgg aaaagtacgg gggacatgag caggcagcgg
      661 ggttgacatt aaagggagac aggattgatg accttaggcg aaggattaat gaatatgcgg
      721 attcggtact tgataagtcg gatcttgttc gtaaggttaa ggtcgatgca aacataaaaa
      781 aagaagatat tgtccttgaa aatgtcagaa tgcttgagct tttggagcct ttcggacaaa
      841 gcaatcctca gccgttactt agatatgaca atatagtaat taatgggata cgtgctgtag
      901 ggcagaataa gcatgcaaaa atcagattta acgacgatgg cttcattatt gaagcaatag
      961 ggtttaatat gggtgaaatg gcaaaggttt atgccgaatc ggatgttgtt gatgttgtat
     1021 gctctctgga agcaaatttg tggaacggta atgaaagtgt gcaactgaat ataaaagatt
     1081 tgcggcccgg atacaaggtg atcagggaaa acaagtttta tctgagtctt gacaaaagtg
     1141 tagatttcgg tttttcaaag aaggatgatg cggttgatga ggtgttgtcg ggaattagca
     1201 aaattgactc caggatagat attgctgaag cagtaaatga atttctttca agaaatgaaa
     1261 gagtagcaat tcttgtaaac tccattaacg gcttacacag catcgaatct gccttggaga
     1321 gaaacttgct tgcgattagg gataaataca tgacatacta tttatatgcg aaacctgagg
     1381 acagcagacc tgtatccgta attgtaaatc cttatccggt gaaggagtac ttttcaaact
     1441 tcgacagggt gatactgtat ggcgaatgga catcaaaaca ttatttgtat aatattgtaa
     1501 gattaattga tttgaacagg gtttatgttt ataatagaat aataattaca tttaatgatg
     1561 atgatatttt gttgaaaaga caggatatag cagcggtatt taaatatttg aaatcgaact
     1621 atgacaggga tattattatt gaaaatctgt ttgtcttagc cagaggtatt gagagacagt
     1681 ttagtattcc aatgaattat ttcaaagtaa aaagggccat acaggtttta gaagaaatct
     1741 ccatactgac aaaaatgccc tttgggaaat atgggctcag gattacaatg ttagacaccg
     1801 ggaaagaaaa gaagaatctt gaggattctt gcctttttag aagtattcgg actttattga
     1861 gcagttgagg attgatgtaa ataaaaacat ttattctgtt acagggagga gtttgtatgg
     1921 atttaaaatc taaattgaga gaggtaatga attttccaaa ggaaggaata aactttattg
     1981 acattacgac agtgcttcag gatgcacaag cattaaagca atgcattgat gcgatgaaag
     2041 aaaagattga gacctttgga gacttcgatc tcattgtagg accggaatca agggggttta
     2101 tttttggaac tcctctggca tacgccatgg gaaaaggctt tatacctatt agaaaaaaag
     2161 gcaaactccc atatcaaaca atcaatgtgg agtatcagct tgagtatggc aaggactttc
     2221 ttgagatgca tatagatgct gtaaaagagg gacagaaagt tgttattgtt gatgatctcc
     2281 tcgcaacagg cggaactact gaatcaaata tcaaactggt agaacagttg gggggagaag
     2341 tagtgggaat agtctatttt gttgagcttt cgttcctaaa cggaagagac aggttaaaa
//
LOCUS       DAOOJC010000378         2398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_34689_length_2398_cov_2.882629, whole genome shotgun sequence.
ACCESSION   DAOOJC010000378 DAOOJC010000000
VERSION     DAOOJC010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34689_length_2398_cov_2.882629"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PLA01_09200"
     CDS             complement(<1..404)
                     /locus_tag="PLA01_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPD01507.1"
                     /translation="MKFHTEYLYFNTKKRREYINITGEVEKALENSGIKEGMVLVSAA
                     HITAGVYINDAEDGIIQDIDDMLERLAPTGPDYRHHRTGEVNGDAHLKNILVGHQVII
                     PVTNGRLDVGPWQQVYYAEFDGQRRKKLIIKV"
     gene            complement(453..1361)
                     /gene="ftsY"
                     /locus_tag="PLA01_09205"
     CDS             complement(453..1361)
                     /gene="ftsY"
                     /locus_tag="PLA01_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPD01508.1"
                     /translation="MGFFDRLKEGLKKTKNAITERIDQVLVSFGKIDEELFEELEEIL
                     ITSDVGIDTSMKIIENLKKKVKENKVVDPQKVKDLLKQEISEILSMGESELRLESTPS
                     VILVVGVNGVGKTTSIGKIANLLKSQGKKVVLAAGDTFRAAAIDQLEIWSGRVGVELI
                     KHSEGADPAAVIYDAIQAVKARKADVLICDTAGRLHTKKNLMEELKKVFRVIDRELPN
                     ASREVLLVLDATTGQNAISQAKSFGDITDVTGIVMTKLDGTAKGGIVIAVASSLNIPV
                     KFIGVGEQMDDLQSFNAKEFAEALFS"
     gene            complement(1400..>2398)
                     /locus_tag="PLA01_09210"
     CDS             complement(1400..>2398)
                     /locus_tag="PLA01_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPD01509.1"
                     /translation="AEKKRNEQEILSLEEKNIGLEQQIKRYEEAKSGKTFEIDRITEE
                     KKVREEESADIVNKITEINKNILLLQEECNRIEVKKARVESDMEAIQNRMWDEYELTY
                     TNALELKKDIGSITQAQKKINELRSSIKELGPVNVAAIEDYIKTKERFEFMSVQKNDM
                     EQAEKKLYRVISEIVAKMKTQFMEQFKLINENFNSVFRELFDGGRAELILLDKENVLE
                     SGIEIEVQPPGKKLQNLMLLSGGERAFTAIALLFAILRLNPTPFCVLDEIEAALDDAN
                     VYKFAQYLKKYSNVTQFAVITHRKGTMEAADALYGVTMQEHGVSKVVSLKMGEKVS"
BASE COUNT          613 a          639 c          351 g          795 t
ORIGIN      
        1 atcaccttta taataagctt ctttcttctc tgcccgtcaa actccgcata ataaacctgc
       61 tgccacggtc ccacatcaag ccttccgttc gtgacaggaa ttatgacctg atgacccaca
      121 aggatgttct taagatgggc atccccattg acctcacctg tcctgtggtg cctgtaatcc
      181 gggccggtag gtgcaagacg ctccaacata tcatcaatat cttgtattat tccgtcctca
      241 gcatcattaa tataaacacc agcagtaata tgggctgcgg aaacaagaac catgccctcc
      301 ttaattccgc tattttcaag ggctttttcc acttcacccg taatatttat atactctctt
      361 cttttctttg tattaaaata taaatactcg gtatgaaact tcatactctc cctccttaat
      421 tatgctttaa ataccttctg ttttgccgct ccttaagaga aaagtgcctc agcaaattct
      481 tttgcattaa acgattgaag gtcatccatc tgttcgccaa cacctatgaa tttaaccggt
      541 atgttaagag atgatgccac tgcaatgaca ataccgcctt tggcagttcc gtcaagcttt
      601 gtcatcacaa ttccggtaac gtccgttata tcaccgaaag acttggcctg ggaaatggca
      661 ttctggcccg tagtggcatc aaggacaagc aaaacctccc ttgaagcatt aggcaattcc
      721 cggtcaatca cgcgaaatac cttttttagc tcttccatca aattcttctt tgtatgaagc
      781 ctccctgcag tgtcgcatat caagacatca gccttgcggg cttttaccgc ctgaatagca
      841 tcataaataa ccgcagccgg gtctgcccct tccgagtgct ttattaattc cactccgacc
      901 cttcccgacc agatttccaa ttgatctatg gcagcagccc tgaatgtatc ccctgcggca
      961 agtaccacct ttttaccctg agattttaaa agatttgcaa tcttacctat ggaagtggtc
     1021 ttgcccacac cgttaactcc aaccaccaaa ataactgacg gtgttgactc aagcctgagt
     1081 tccgactcac ccatacttaa aatctcagat atctcctgct tcaatagatc cttaaccttt
     1141 tgaggatcga caaccttgtt ctccttaacc ttctttttaa gattttcgat gattttcata
     1201 ctggtgtcaa ttccaacatc cgaagttatt agaatctctt ctaactcctc aaacagttcc
     1261 tcatcaatct ttccaaagga aaccaacacc tgatctattc tttcggtaat tgcatttttt
     1321 gttttcttca atccttcttt tagtctgtca aaaaaaccca tattctagcc tccatttaaa
     1381 attatattta aatttaatat tagctcactt tttcacccat cttaagagaa accacctttg
     1441 atactccatg ctcctgcatc gtcacaccat acaaggcatc agccgcctcc atggttccct
     1501 ttctatgagt aatgaccgca aactgtgtca cattcgagta cttctttaaa tactgggcaa
     1561 acttataaac attggcatcg tccaaagcag cttcaatctc gtcaagtaca cagaaaggag
     1621 tgggattcag ccttaggatt gcaaaaagca atgctattgc ggtaaacgcc ctctctcctc
     1681 cagaaagcaa cataagattc tgaagcttct ttcccggagg ctgcacctct atttcgatac
     1741 cgctttcaag aacattttcc ttgtccaata aaatcagttc tgccctacct ccatcaaaga
     1801 gttcgcgaaa tacactattg aaattctcat ttatcaattt aaactgttcc ataaactgag
     1861 tcttcatttt tgccactatt tcgctgatta ccctgtaaag ctttttctct gcctgttcca
     1921 tatcattttt ctgaaccgac ataaactcaa accgttcttt agtcttgata taatcctcaa
     1981 tagctgccac attaaccggt ccaagctctt ttatactgct tcttagttca tttatttttt
     2041 tctgtgcctg ggttatgctt ccaatatcct ttttcagctc caatgcattg gtatatgtca
     2101 gctcatactc atcccacatc ctgttttgta ttgcctccat atccgactct accctggcct
     2161 tcttaacctc aatccggttg cattcctcct gaagcaaaag tatgttttta ttgatttcgg
     2221 ttatcttatt tacaatatct gcggactctt cctcccgcac ctttttctcc tctgtaatcc
     2281 tgtctatctc aaaagttttt cctgatttgg cttcttcata ccttttaatt tgctgctcca
     2341 atcctatgtt tttctcttct aatgacaata tttcctgttc attgcgtttt ttctccgc
//
LOCUS       DAOOJC010000379         2396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_34731_length_2396_cov_3.679197, whole genome shotgun sequence.
ACCESSION   DAOOJC010000379 DAOOJC010000000
VERSION     DAOOJC010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34731_length_2396_cov_3.679197"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="PLA01_09215"
     CDS             <1..1026
                     /locus_tag="PLA01_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HPD01510.1"
                     /translation="ISYICNCYSKATVTATNTPKATPTATPTGKATPTPSATPTPRPA
                     PTAVKNGNGSGLSGEYYDNMDLTKFKFAKVDPVINFDWGEGSPADAIDADSYSIRWTG
                     KVEPRYSETYTFYTNTDDGVRLWVNGVLLIDKWQSQSATEYSGKIDLEAGKKYEIKME
                     YYEHRRGASAKLMWSSLSQEKEIIPSSQLYPSDEPLPPQPENGLKAEYFGDKELGELR
                     LGRTDDVIDFDWQRNYPVSDLQEGRFSVRWTGKIQSRYTEEYTIHALSDDGVRVWIDN
                     ILIIDNWKRQKSRVETTGKFEFKEGKQYSIKIEYFNYGGPASMKLLWSSTRQEKQVIP
                     SKYLFTF"
     gene            complement(1286..2050)
                     /locus_tag="PLA01_09220"
     CDS             complement(1286..2050)
                     /locus_tag="PLA01_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPD01511.1"
                     /translation="MKNKAVLTLVVSILLFILYTIWNNILILDDFHPTNEDLYVISES
                     FQDEFTESYIQKEGYHIYINLDELRLYLYKDGVLVKTYLVSGGKPETPSPEGTWKIIS
                     KSAWGGSFGGSWMGLNVPWGKYGIHGTKYPWFIGKQNASHGCIRMYNESAKELYSIVP
                     HGTTVTIVQKNRPFKVLKSGDVGSDVLKVQKALKKLGYFNDWPSGKFGDNLKKSVIKF
                     QKTNNYKVTGTVSKRLYDIIIQKYEEKIREEQNQFE"
BASE COUNT          771 a          422 c          494 g          709 t
ORIGIN      
        1 atatcatata tatgcaattg ttattcaaaa gctacagtta cagcaacaaa tacaccaaaa
       61 gcaacaccaa cagcgacacc gacaggaaaa gcaacaccaa caccttcagc aacaccaaca
      121 ccgagaccgg ctccgacagc tgtgaaaaac ggaaacggca gcggacttag tggcgaatac
      181 tatgacaaca tggatttgac taagtttaag tttgcaaaag ttgatcctgt tattaatttc
      241 gattggggtg agggttcacc ggcagatgca attgatgcgg attcttattc tataagatgg
      301 acaggcaagg ttgaaccccg gtattcggaa acatacactt tctataccaa taccgatgat
      361 ggggtaagac tctgggtaaa cggtgttctg ctgattgata aatggcagag tcaatcagct
      421 accgagtaca gcggcaagat tgaccttgag gcgggcaaga agtatgaaat taaaatggaa
      481 tactatgagc atagaagggg tgcatcggca aagctcatgt ggtcgagttt gagtcaggag
      541 aaggagataa taccgtcgag tcaattgtat ccttccgatg agccattgcc tccccaaccg
      601 gaaaatggct tgaaagcaga gtattttggc gataaggaat tgggtgaatt aaggcttggc
      661 agaacggacg atgttataga ttttgattgg cagaggaatt accctgtctc agacttacag
      721 gaaggaagat tttcagtaag gtggacgggt aaaattcaat caagatatac cgaggaatat
      781 acgattcatg cactttcgga tgatggtgtc agagtatgga tagacaatat tctcataatt
      841 gataattgga aaaggcagaa gagcagggtt gaaactacag ggaaatttga gtttaaggaa
      901 ggaaaacaat acagtataaa aattgagtat tttaactatg gcgggcctgc gtccatgaag
      961 cttttatggt caagcacaag gcaggaaaag caggttatac cttcgaaata tctgtttaca
     1021 ttttaataag aggaatacgg tatagaattc atggtgtaaa ttagggtgaa ttaaataaat
     1081 gtgaaggaaa gtattccaga aacttaagtt acgcaagact agccgcttgt aaatactccg
     1141 tggggtcgag caaaactcac ttcgtgaaag tggatgctct ccctttccac gtcgtattaa
     1201 aaggcggcta caacttgctt cacaatgttt cttccatatt ttacttcact taaataatat
     1261 acacactgat tagtttagat tgtgtctatt caaattgatt ttgctcttct ctaatctttt
     1321 cttcatattt ttgaataata atatcataaa gccgtttgct taccgtaccg gtaactttat
     1381 agttatttgt tttttgaaac tttataacac ttttcttaag attatctccg aatttaccgc
     1441 tgggccagtc attgaagtac cctaattttt ttaaggcctt ctgaacttta agtacatcgg
     1501 agccaacgtc tccgctcttt aaaaccttga aggggcggtt tttctgaaca atagtgacgg
     1561 tagtgccatg ggggactatg ctatagagct ccttggcact ttcgttatac attctgatgc
     1621 agccgtggga agcattttgt tttccgataa accagggata ctttgttccg tgaattccgt
     1681 attttcccca gggtacgtta agtcccatcc atgaccctcc aaaacttcca ccccaggcag
     1741 acttgcttat tatcttccac gttccttcag gagaaggggt ttcaggtttt ccaccggaca
     1801 ccaaataggt tttcaccagt actccgtcct tgtaaaggta taacctcagc tcatccaaat
     1861 taatataaat atgatagcct tctttttgta tataggactc tgtaaactca tcttgaaaac
     1921 tttcactgat tacatacaga tcttcgtttg taggatgaaa gtcatccaga atcaatatgt
     1981 tgttccatat tgtatataaa ataaacagaa gtatagacac taccaaggtc aacacggctt
     2041 tatttttcaa taatatcacc taagatttac tgataactgt taaattatat gatttacaat
     2101 gcttggacta ttatgataag cacaaaaagc cgataactaa ataaaacaaa ttgtattatt
     2161 gtactatgaa cataaaccta tgtttgatta atagatgaat attagttaaa ctattatgag
     2221 gaaatagatt gtatataata ttgtataatg ttaaaattcg attaggatta ttttataagg
     2281 agggaattta tatgtctaag gccattagag aacagatagt aaaggtgtca aagctcatgt
     2341 acgaaaggga tatggcaaat gcttttgaag gtaatgtgtc cgtacgggat gataat
//
LOCUS       DAOOJC010000380         2387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_34898_length_2387_cov_3.874357, whole genome shotgun sequence.
ACCESSION   DAOOJC010000380 DAOOJC010000000
VERSION     DAOOJC010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34898_length_2387_cov_3.874357"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLA01_09225"
     CDS             complement(<1..214)
                     /locus_tag="PLA01_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01512.1"
                     /translation="MKLIVHNIKMSLDDGMDELKEFAIKKAGVKENNVNDFKIVKESI
                     DARKKPHIHIVYSVMLQVQNGTRIRKD"
     gene            complement(276..1523)
                     /locus_tag="PLA01_09230"
     CDS             complement(276..1523)
                     /locus_tag="PLA01_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HPD01513.1"
                     /translation="MEGEGQMSELLKKDTTIKILSVVFAIFLWYFVLDSTNPVEWIDL
                     NVPLSVENENVLKEKGIIIKDGNFPRTVSVRLKGRKNKINTVSQNDFEAILDLSKINE
                     SNTRHLQIDVRYQGIDVFNYKGGVYFQGVTPRHVDLVLEKVGENSFPVKVIPTGNPRE
                     NYKVISVNAVPGTVSIEAADDIVDSIGEVCAYVDINNMSSNMVRKSECVVYDTEGEEI
                     EELTGKLSVDVSVEFAKEVPIVPTIIGRPARNYADGIHRVIPEKALITGAADTVELID
                     NLKTEPIDIENMSATTTKIVNLVLPEGVRLFDTQRSVYVDVVIEEVVVKEITFDKDDI
                     LIENAQAGDMYKCEVLNENIKISITGIRQEIEKIKESNLRLSVDVRGLGEGRHKRPLK
                     VVLPDTVKLLQDYEVEVNISKMN"
     gene            complement(1502..2377)
                     /gene="cdaA"
                     /locus_tag="PLA01_09235"
     CDS             complement(1502..2377)
                     /gene="cdaA"
                     /locus_tag="PLA01_09235"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HPD01514.1"
                     /translation="MILLIGGSIWEFIKYLSTNLDINSPWDVIKIIIDISIVSFVVYK
                     LVKLIRETRAWQLIKGILVIVVAARLSEILGFRTISFILRLTIEYMAIILVVLFQPEF
                     RRGLEQLGRSRFRNFFSLEDEDINVKVKALIEEIVKAVTEMSKTFTGALIVIERETKI
                     GEIINSGIRLDSNVTSELLINIFTPNTPLHDGAVVIRDNKIKAAACFLPLTENSNLSK
                     ELGTRHRAALGISEVSDSIVVVVSEESGKISVALNGGLSRNLTSDTLRKALSKNLIMK
                     DNPGKRLGIWKVKAK"
BASE COUNT          670 a          576 c          331 g          810 t
ORIGIN      
        1 tgtcctttcg tatccttgta ccgttttgaa cttgtaacat gacagagtat acaatatgaa
       61 tatgcggctt ttttcttgca tcaatagact ctttgactat tttaaagtca tttacgttat
      121 tttcttttac tccggctttt tttattgcaa actcttttaa ttcatccatg ccgtcatcta
      181 aagacatctt tatattatga acaattaact tcatcgatac ctctcaaatg caatatcatc
      241 tattgtaaat aaaattcttc cacgatttag tcaaactaat tcattttgct tatattgact
      301 tctacctcat agtcttgcaa aagctttacc gtatcgggca gtacaacctt tagaggcctt
      361 ttgtgacgac cttctcccag gcccctcacg tctacactta aacgcaagtt ggactctttt
      421 atcttttcaa tttcttgccg tatacccgtt atgcttattt ttatattttc attgagaact
      481 tcacatttat acatatcgcc ggcctgagca ttttcaatca atatatcatc cttgtcaaag
      541 gtaatttcct taactactac ctcctctata accacatcca catatacgct cctttgagta
      601 tcaaaaagac gtacaccttc gggcaaaaca agattcacta ttttagtcgt ggtggcagac
      661 atgttttcaa tatcaatggg ttcggtcttc aggttgtcaa taagttcaac cgtatccgcc
      721 gcccctgtta tcagtgcttt ctccggtata actctatgta ttccatcggc ataatttctt
      781 gcaggccttc cgataatcgt aggtactata ggcacctctt tagcaaactc aacactgaca
      841 tccacactaa gctttccggt tagttcttct atttcctcac cttccgtatc ataaaccacg
      901 cattccgatt ttctaaccat gttactgctc atgttgttta tatccacata tgcacagact
      961 tctccaattg aatctacaat atcatccgca gcttctatcg ataccgttcc cggtactgca
     1021 tttacactta ttaccttata attctccctt ggattgccgg taggaataac cttaacagga
     1081 aaagagtttt ctccaacctt ttcaagtaca aggtcaacat gcctcggggt tacaccttga
     1141 aaataaaccc cacctttgta gttaaacaca tcaatcccct gatacctcac atctatttgc
     1201 agatgtctgg tatttgactc atttatcttt gaaaggtcaa gaattgcctc aaaatcattt
     1261 tgacttactg tatttatttt atttttcctt ccttttaatc tgacggaaac ggttctggga
     1321 aaattgccgt cttttataat aattcccttt tctttgagta cattttcatt ttccacactc
     1381 agaggtacgt ttaaatcaat ccactctacc gggttggtgc tgtccaagac aaagtaccat
     1441 aaaaatatgg caaataccac cgacaatatt ttaattgtag tgtccttctt caataattca
     1501 ctcatttggc cttcaccttc catattccaa gccttttacc cggattatcc ttcattatga
     1561 ggtttttact cagtgccttt ctgagagtat ctgaagtaag attccttgaa agtcctccgt
     1621 ttaatgcaac ggaaatcttt cccgattcct cggaaaccac aactacaatt gaatcagaga
     1681 cctcacttat tcccaatgcc gccctgtgtc tggttcccag ttccttactc aggtttgaat
     1741 tctcggtcaa aggcaggaaa cacgccgctg cctttatttt attatcccta atgacaacag
     1801 ctccgtcatg caaaggagta tttggcgtaa aaatatttat caaaagctct gatgtaacat
     1861 tcgaatccaa acgaataccg gagttgataa tctctcctat cttagtttcc ctttcaatta
     1921 caataagtgc tccggtaaag gtcttcgaca tctctgttac cgcttttact atctcttcaa
     1981 tcaatgcctt tacctttaca tttatatctt cgtcctcaag actgaaaaag tttctgaacc
     2041 ggcttcttcc caattgctcg agacctcttc tgaactcggg ttgaaataaa accaccagaa
     2101 taatagccat gtattcaata gtaagcctca atataaagga aatggttcta aaacctagta
     2161 tctcactcag cctggcagcc actacgataa caagaatacc tttgattaac tgccacgccc
     2221 gagtctccct tatcagcttt acaagcttgt aaacaacaaa agagacaata gagatatcaa
     2281 ttattatctt aatgacatcc cagggactat ttatatcaag atttgttgat aaatatttaa
     2341 taaactccca tatacttccg ccaatcaata atatcatttc aaaaccc
//
LOCUS       DAOOJC010000381         2385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_34956_length_2385_cov_2.235622, whole genome shotgun sequence.
ACCESSION   DAOOJC010000381 DAOOJC010000000
VERSION     DAOOJC010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34956_length_2385_cov_2.235622"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PLA01_09240"
     CDS             complement(<1..599)
                     /locus_tag="PLA01_09240"
                     /inference="COORDINATES: protein motif:HMM:NF018406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 family protein"
                     /protein_id="HPD01515.1"
                     /translation="MENKRKPVFWIFIKSFTDMHAYKHFVYKNFSKAFLYILLFTFLL
                     SVIFSAIFVASIKDYTTQLITQLEQNTPEFTIKNGELVIESDQPVILAETDDKGTLII
                     IDKEYTGSVTKYEEYDTVIVLGQKEIYLSNSAIRYSIGYQSVLGQKQEVTSEMLFGRV
                     SSSKPYLYIFFFVSMFVFFIGNYLFYDLFIALIVNTIRR"
     gene            complement(701..1021)
                     /locus_tag="PLA01_09245"
     CDS             complement(701..1021)
                     /locus_tag="PLA01_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009446011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPD01516.1"
                     /translation="MRGINERIKKLRTQMNLSQDYVAKYLGVSRSTFTQMENGNRKIL
                     ADEISKLSTLFGVSVNAILDESELSQPATVFARSFEKLDESDQAEIMNLIRFKEQMKA
                     QRTK"
     gene            1136..>2385
                     /locus_tag="PLA01_09250"
     CDS             1136..>2385
                     /locus_tag="PLA01_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPD01517.1"
                     /translation="MSRRDKEFRSDKFVLQSDFRPTGDQPAAIEALANGVLSGYKHQT
                     LLGVTGSGKTFTMANIIEKVQKPTLVIAHNKTLAAQLCSEFKEFFPDNSVEYFVSYYD
                     YYQPEAYIPSTDTYIEKDMSINDEIDQLRHAATSALLQRRDVIIVASVSCIYGLGSPA
                     DYEDLMLYIGVGMTLDRDDVIKKLVEIQYSRNEMDFKRGTFRAKGDSLEIAPSNSANV
                     GYRISFFGDEVEKITQFDSLTGEITGYVNHLAVFPASHFVSKDTKLKRAIEDIQAELQ
                     ERVAYFENQGKIVEAYRLNQRTSYDLEMIRNIGFCSGIENYSRHIDGRKEGQPPATLL
                     SFFPEDFLMFIDESHVTIPQIGGMNKGDRSRKKNLIDFGFRLPSAYDNRPLTFEEFKE
                     KQNQCIYVSATPGDYELGISEQVA"
BASE COUNT          727 a          449 c          491 g          718 t
ORIGIN      
        1 atacgtctta ttgtattaac tatcaatgct atgaacaaat catagaagag gtagttaccg
       61 ataaagaaaa caaacataga tacaaagaag aaaatataaa ggtagggttt tgatgaagat
      121 actctgccga acaacatttc cgaagtaact tcctgttttt gacctaaaac cgattggtaa
      181 cctatactgt accttatagc cgaattggat aaatatattt ccttttgtcc taaaactata
      241 acagtatcat attcttcgta cttggtaacc gatcccgtat attctttatc gattattata
      301 agcgttcctt tatcatcggt ttccgcaaga atcaccggtt gatcactctc tattacaagc
      361 tctccgtttt tgatggtaaa ttccggagta ttttgttcca gttgcgttat aagttgggta
      421 gtgtaatctt taattgatgc tacaaatata gccgagaaaa taacggaaag cagaaaagta
      481 aacagcagaa tgtataagaa tgcttttgaa aaattcttat acacgaagtg tttatatgca
      541 tgcatgtccg taaagctctt tataaatatc cagaataccg gttttctttt gttttccata
      601 gttctccttg cgttcacaat tttacttata ccattataca gcataatgac aaaaagatat
      661 aaaaaaatgc agcctactat tttcaccatt tcaaatcgta tcatttagtc ctctgtgcct
      721 tcatttgctc cttaaagcgg ataaggttca tgatttcagc ttgatcggat tcatcgagtt
      781 tctcaaaact tctggcaaaa actgtggccg gttggctcag ctcactttcg tccagtattg
      841 cgttgactga cacgccaaat aatgtgctca gttttgagat ttcgtcagca agtatttttc
      901 tgttgccatt ttccatctgg gtgaaagtag acctgctcac accaagatat tttgctacat
      961 agtcctggga cagattcatc tgtgttctca gttttttaat tctctcatta attcccctca
     1021 tttttgctcc tcctttacca acagtattat tataatacgc gctgtttgaa aatacaacac
     1081 taaatgttag aaaacctaac ccaatttttt atgtgttgtg tgctaaaata attacatgag
     1141 caggagagac aaagagttca gatcagataa atttgttttg caatcggatt tccgacctac
     1201 aggtgaccag cctgcagcaa tagaggcatt ggcaaacgga gtgctttcag ggtataaaca
     1261 tcagactctt ctgggagtta ccggttcggg taagactttt accatggcaa atattattga
     1321 aaaagttcag aagcccactc ttgtcattgc acataacaag acattggctg cccagctgtg
     1381 ttcggagttt aaagagttct ttcccgataa cagtgtggaa tactttgtat cctattacga
     1441 ctattatcag cccgaagcat atattccctc taccgataca tatattgaaa aggatatgtc
     1501 cataaatgac gaaatagatc agctgcgaca tgccgccaca tcggctcttc tgcaaagaag
     1561 ggatgtaata atagtagcat cggtttcctg catttacggt ctgggcagtc ctgccgatta
     1621 tgaggactta atgctgtata tcggggtggg catgacgctt gacagggatg atgtgataaa
     1681 gaaactggtg gaaatacagt actcacgaaa tgaaatggat tttaaaagag gaactttcag
     1741 ggcaaaagga gattctttag aaattgctcc ttccaacagt gcaaatgtcg ggtacagaat
     1801 aagctttttc ggagatgaag tggagaaaat tacgcagttt gattcattga ccggagaaat
     1861 aacaggttat gtcaatcatc tggcggtttt tcccgccagt cattttgttt ctaaagatac
     1921 aaaacttaaa agagccatag aagatataca ggcggaactg caagagaggg tggcatattt
     1981 tgaaaatcaa ggcaagatag tggaggctta caggctcaat cagagaacca gttatgattt
     2041 ggagatgatt aggaatatag ggttctgttc gggaatagag aattattcaa ggcatattga
     2101 cggcaggaag gaaggacagc cgccggccac tttgctttcc ttcttccccg aggatttttt
     2161 aatgttcata gatgagtcgc atgtaacaat tcctcagata ggaggtatga acaaaggcga
     2221 tcggtccagg aagaagaacc tgattgactt cggattccgg ctgccttctg catatgacaa
     2281 cagaccgctg acttttgagg agtttaagga aaaacagaac caatgtattt atgtgtcggc
     2341 aactcccggc gattacgagc tggggatatc ggagcaggta gccga
//
LOCUS       DAOOJC010000382         2373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_35194_length_2373_cov_3.050906, whole genome shotgun sequence.
ACCESSION   DAOOJC010000382 DAOOJC010000000
VERSION     DAOOJC010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35194_length_2373_cov_3.050906"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..410)
                     /gene="rplX"
                     /locus_tag="PLA01_09255"
     CDS             complement(66..410)
                     /gene="rplX"
                     /locus_tag="PLA01_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPD01518.1"
                     /translation="MKKKVHVKKGDTVIVLNGKDEGKKGKVLEVNAQDGTVLVEGVNI
                     TTKHKKPRSQYQQGGIIHQESPINSSKVMLVCPKCGKPTRIRRHILENGEKDRECKKC
                     NGTIDIIKEAKG"
     gene            complement(429..797)
                     /gene="rplN"
                     /locus_tag="PLA01_09260"
     CDS             complement(429..797)
                     /gene="rplN"
                     /locus_tag="PLA01_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPD01519.1"
                     /translation="MIQAQTMLRAADNSGAKELMCIKVLGGSKRKYANIGDVIVASVK
                     EATPGGVVKKGDVVKCVIVRSKRGIRRTDGSYIRFDENAAVIIKEDKNPRGTRIFGPV
                     ARELRDKEYMKILSLAPEVL"
     gene            complement(822..1079)
                     /gene="rpsQ"
                     /locus_tag="PLA01_09265"
     CDS             complement(822..1079)
                     /gene="rpsQ"
                     /locus_tag="PLA01_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPD01520.1"
                     /translation="MADRALRKTRVGKVTSNKMDKTIVVNIESSEKHPLYKKYVKRTY
                     KLKAHDEKNDCNIGDTVRVMETRPLSKDKRWRLVEIIERAR"
     gene            complement(1106..1309)
                     /gene="rpmC"
                     /locus_tag="PLA01_09270"
     CDS             complement(1106..1309)
                     /gene="rpmC"
                     /locus_tag="PLA01_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPD01521.1"
                     /translation="MKAKDIKERSLDELEKELGELKSELFKLRFQHATNQLENPMKLK
                     DVKKSIARIKTVLRERELKGIKV"
     gene            complement(1299..1736)
                     /gene="rplP"
                     /locus_tag="PLA01_09275"
     CDS             complement(1299..1736)
                     /gene="rplP"
                     /locus_tag="PLA01_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPD01522.1"
                     /translation="MLMPKRVKRRKVQRGRMKGVATRGNKVYEGQFGLQALEPAWITS
                     NQIEAARVAMTRFTKRGGKVWVKIFPDKPVTQKPAETRMGSGKGAPEYWVAVVKPGRV
                     LFEIAGVSEEVAREALRLAMHKLPIKCKFITREEEMGGEANES"
     gene            complement(1736..2371)
                     /gene="rpsC"
                     /locus_tag="PLA01_09280"
     CDS             complement(1736..2371)
                     /gene="rpsC"
                     /locus_tag="PLA01_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPD01523.1"
                     /translation="MGIIKDWDTKWYANDKNFSELLVEDVKIRKFIKKKLYISGISRI
                     EIERAANKVKINVNTSKPGLVIGKGGSGIEELRKQLEKLTQKNVLINITEIKVPELDS
                     QIVAENIASQLEKRISFRRAMKQAMSRTMRLGAKGIKTSVSGRLGGAEIARTEHYHEG
                     TIPLQTLRADVDYGFAEANTTYGKLGVKVWIYKGEVLPAVKKEKKREGGDK"
BASE COUNT          573 a          603 c          305 g          892 t
ORIGIN      
        1 tctttaacat acttatcttt taaagcagac atctaattat tacctcctct ctcagagaat
       61 acctatcaac ctttggcttc tttgattata tcaatagttc cgttgcattt tttgcactct
      121 ctgtcctttt ctccattctc cagaatatgt cttctaatcc ttgtaggctt tccgcatttc
      181 gggcaaacca gcataacctt tgagctgtta atcggtgatt cctgatgaat tattccaccc
      241 tgctggtatt gtgatctggg ctttttatgt ttagttgtaa tattaacacc ctctactaaa
      301 acagtgccgt cctgtgcatt aacctccaac actttaccct ttttgccttc atcttttccg
      361 tttaaaacta ttactgtgtc tcccttttta acgtgaacct ttttcttcaa cttaagccgc
      421 ctcctccctt ataaaacttc tggtgcgagg gacaatattt tcatatattc cttatccctt
      481 aattctcttg caacaggtcc aaagatacga gttcctcttg gattcttatc ctcttttata
      541 ataactgctg cattttcatc aaaccttata taggaaccat ctgttcttct aatccctctt
      601 tttgaacgaa ctataacaca ttttacaacg tcaccttttt taacaacacc tccgggtgtt
      661 gcttctttaa cggaagctac tataacgtca ccaatgtttg cgtactttct ttttgaacct
      721 ccaagaacct ttatacacat taactccttt gctccgctgt tatcagcagc cctgagcatt
      781 gtttgtgctt gaatcatatt agaacctcct ttctgctaag cttaacgggc tctttcaata
      841 atctcaacca gtctccacct tttatctttg ctaaggggac gagtttccat taccctgact
      901 gtgtcaccga tattacaatc atttttctca tcatgagctt tcagtttgta tgttctttta
      961 acatacttct tataaagcgg atgcttttca gaactttcta tattaacaac tattgtttta
     1021 tccatcttat tgctggtaac cttaccaacc ctagtctttc ttaaagctct atcagccaac
     1081 ttaacctacc tcctttcaag gtatattaaa cctttatacc cttgagttcc ctttccctca
     1141 atacagtctt aatacgtgca attgacttct ttacatcctt gagtttcata ggattttcaa
     1201 gctgatttgt tgcatgttga aatctaagct tgaataactc tgacttcaac tcaccaagtt
     1261 ccttttccaa ctcatccagt gatctttctt ttatatcttt agctttcatt tgcttcacca
     1321 cccatttctt cttcacgtgt tataaattta cacttaatgg gaagtttatg catagcaagc
     1381 cttagagctt cccttgcaac ctcttccgat actccagcaa tttcaaacaa tactctacca
     1441 ggcttaacta cagccaccca atattccgga gctcctttac cactacccat acgggtttca
     1501 gcaggctttt gagttaccgg cttatccggg aatatcttaa cccaaacctt accgcccctt
     1561 tttgtaaaac gagtcatagc taccctggca gcctctatct gattactcgt tatccacgca
     1621 ggttcaagag cttgaagtcc aaattgtcct tcatacactt tgtttcctct tgttgccaca
     1681 cctttcattc ttcctctttg aacctttctt cgttttactc ttttaggcat taacattatt
     1741 tatctcctcc ttcccgcttt ttctccttct taacagccgg aagaacttct cctttatata
     1801 tccagacttt aacacccaat ttaccgtagg ttgtattagc ttctgcaaaa ccgtaatcaa
     1861 catctgccct taatgtctga agaggtattg ttccttcatg gtaatgctct gttcttgcta
     1921 tttcagcacc tccaagacga ccggacacag atgtttttat accctttgca ccaagtctca
     1981 ttgtccttga catagcttgt ttcattgctc ttctgaaaga gattcttttt tcgagctgtg
     2041 aagctatatt ttctgcaact atttgagaat ccaattccgg aaccttaatt tctgttatat
     2101 ttatcaaaac attcttctgg gttaattttt cgagctgttt tctcagttcc tctattccgg
     2161 aaccaccctt tccgattact aatccgggtt ttgatgtatt aacattaatc ttaaccttat
     2221 ttgcagctct ttcaatctca attcttgaaa tacccgaaat atatagcttc tttttgatga
     2281 acttccttat ttttacatct tcaaccaaaa gctcgctaaa attcttgtca ttggcgtacc
     2341 acttggtatc ccaatccttg attataccta ttc
//
LOCUS       DAOOJC010000383         2371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_35234_length_2371_cov_3.331174, whole genome shotgun sequence.
ACCESSION   DAOOJC010000383 DAOOJC010000000
VERSION     DAOOJC010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35234_length_2371_cov_3.331174"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..817)
                     /locus_tag="PLA01_09285"
     CDS             complement(50..817)
                     /locus_tag="PLA01_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPD01524.1"
                     /translation="MREEDRVTFGNASVSTPDTQTENKTSIDEIKELGKASIKNKKGN
                     IHCLTVIGQIEGHMVLPPQNKTTKYEHVIPQLVAIEESEEIDGLLLILNTVGGDVEAG
                     LAIAEMIASMSKPTVSLVLGGGHSIGVPMAVATKYCFIAPSATMTIHPIRLNGMVIGV
                     PQTYEYFDRMQERVVQFVAKNSKISKDNFRSLMLKTGELANDVGTILFGDEAVKHGLI
                     DETGGLHDALNKLYEFIEEERSKKKEKVYEKELEELQ"
     gene            complement(1043..2332)
                     /locus_tag="PLA01_09290"
     CDS             complement(1043..2332)
                     /locus_tag="PLA01_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01525.1"
                     /translation="MRKRLKTLALSAIIVANLMSFSGCDSLFKKTPEVQPEPKIETPI
                     TSPDEDNKVKEMLKEYFGKIYEKPINEYEQNILSGNIPDSISGMIANRTLEEGNNNPE
                     VGIHLPRMVSINGIVALDYDIIIANDGKPLVDAAFVGDTNDIFVYYVKVDLKAKGLAE
                     SYFLQYYQKNPDTKLYERIKENGQEKEINEEAYDYIKVQARYDVEVIKEGNDYKVLTQ
                     REANYKPGLTKRLFILNNDFLTRIPYLNTQIDEEKKVYDGEKALIELYFNNLLKVDKE
                     RMNLIKSNWNKDKLVFIDILQKIGVSSAEGGKNLLLIDDDYKGKFKIESFPLQINMER
                     LNRYDSFTVSVHPGYSYKNKMYMVSFKAAAVNTNGMIGDETFYQYDYVITMKNVNGTT
                     LIDSVKLNEYFKTTAPAQKTEAKDEKKSDSKDTESKN"
BASE COUNT          656 a          537 c          340 g          838 t
ORIGIN      
        1 tcgaaaaaat ccttaaacac cacttcaggg ggtacaattg agtatagaat cattgcaatt
       61 cctccaattc cttctcatat accttctctt tttttttgct tctttcctct tctataaact
      121 catacaactt attcaaagcg tcatggagac cgccggtttc gtcaataagg ccatgcttaa
      181 ccgcttcatc cccgaacagt attgtaccaa cgtcatttgc cagttcacca gtctttaaca
      241 taaggcttct gaagttgtcc tttgatattt ttgagttttt ggccacgaac tgtaccactc
      301 tctcctgcat cctgtcaaaa tattcatagg tctggggcac accaatgacc attccgttca
      361 gtcttatagg gtgtattgtc attgtagcgg acggagcaat aaagcaatac ttcgtagcaa
      421 cagccatagg tacaccaatg ctgtgccctc ctcctaaaac caaggatacg gtcggctttg
      481 acatgcttgc aatcatctca gctatggcaa gcccagcctc cacatcccct cccacagtgt
      541 ttaatataag cagcagtccg tcgatttctt cactctcctc tatggcaaca agctgcggta
      601 taacatgctc gtatttagta gtcttgttct gcggaggaag aaccatatgc ccctctattt
      661 gacctatcac tgtaaggcag tgaatattac cttttttatt ctttatagat gccttcccca
      721 gttcctttat ctcatctata ctcgtcttgt tttctgtttg ggtatcgggt gttgaaaccg
      781 atgcatttcc aaaagtaacc ctgtcttctt ccctcaaatc accacatcct tagattttta
      841 agatacactt tctttattag tattatttta accaaaggaa aatataacag ggaataatta
      901 agttataaat agcagaacaa aggtgaagga aaataatgca cttaaataaa taacaaaagg
      961 acccgcaatt catcgcaggt ccattctttt ttatattata aacttcaaac tttttttcct
     1021 acttgccttt attgccacct tctcaattct tactttcagt atccttcgaa tcagattttt
     1081 tttcgtcttt tgcttctgtt ttctgtgccg gagcagtagt cttaaagtat tcattcaatt
     1141 ttacactgtc tatcaacgtt gtaccgttta cattcttcat tgtaatcaca taatcatact
     1201 ggtagaatgt ttcatcccct atcattccat tggtattgac agcagctgct ttaaacgaaa
     1261 ccatatacat tttattctta tatgaatatc cggggtgaac cgatacagta aagctgtcat
     1321 atctgtttag cctctccatg tttatctgaa gaggaaacga ttcaatttta aatttgccct
     1381 tatagtcatc atcgataagc aacaaattct ttccgccttc tgcagaactt acacctattt
     1441 tctgcagaat atcaataaaa acaagtttat ctttattcca attggatttt atcaggttca
     1501 ttctttcctt atcaactttc agcaaattgt tgaagtacaa ttcgataagt gccttctctc
     1561 catcataaac cttcttttct tcatcaattt gtgtattaag gtaaggaatt ctggttaaaa
     1621 aatcattatt taaaataaac aatctttttg taagacccgg tttataattt gcctctcttt
     1681 gagtcagaac cttgtaatcg tttccttcct taattacctc tacatcgtat cttgcctgta
     1741 cctttatgta atcataagct tcttcattta tctctttttc ttgtccgttt tccttaattc
     1801 tctcatagag cttcgtatcg ggattcttct gataatactg gagaaaatag gactcagcca
     1861 aaccttttgc cttaagatca acctttacat aatatacaaa tatgtcatta gtgtcgccga
     1921 caaaggcagc atcaaccaac ggcttaccgt catttgcaat aattatgtca taatctaaag
     1981 caacaatacc atttattgaa accatccttg gaaggtgtat acccacttcc ggattattat
     2041 taccttcttc taaggtacga tttgcgatca taccggaaat actgtcaggt atatttccgc
     2101 tgagaatatt ctgttcatac tcatttatcg gcttctcata aattttacca aagtactcct
     2161 ttagcatttc ttttacctta ttatcctcat cgggacttgt aatcggtgtc tcaatctttg
     2221 gttcgggctg aacctccgga gtcttcttaa acaacgagtc acatccggaa aagctcatca
     2281 aatttgcgac aataattgct gataatgcca atgtttttaa tctttttctc atcttatcca
     2341 cctcatattt taaaatatat cagttaaatc t
//
LOCUS       DAOOJC010000384         2363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_35397_length_2363_cov_3.960139, whole genome shotgun sequence.
ACCESSION   DAOOJC010000384 DAOOJC010000000
VERSION     DAOOJC010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35397_length_2363_cov_3.960139"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2138
                     /locus_tag="PLA01_09295"
     CDS             <1..2138
                     /locus_tag="PLA01_09295"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPD01526.1"
                     /translation="EPNVLSMINTGDYYWDPPLGEVFKGTTVKAVAVRSDGKVSRIVT
                     CSYFVDPDMNTRYSLPVISIVTDEANLFDKNTGIYLNSNKSGADWERPAHIEFFEEDG
                     TLGFSHYCGIRLHGGGSKGFGQKSLRLYADRGYDYKEKISYNIFPGLKDKVTGKSITD
                     FKRLILRNSGNDWSNSMFRDGLMHKLVSHLKLDTQAYRPSVVFINGEYWGIHNIRERY
                     DNIYFASHYNLDKDRVALLEVTYFGTLTVNEGTDEDAQAYTNDIINFLKSNDITQKDN
                     YEYIKTKMDMDNFIDYQVANIYFANGDWPQNNVSMWKYKTEDGLYHPEAPYGQDGRWR
                     WIIKDTDFGFCGPMMASSGITHDTLSHASENAQNEWAVFLFKTLLKNSEFRNAFINRM
                     ADYLNTCFEPELVTDTIDEMKNVIESSIPEHNARWQAIFDWDGEVDLMRTFSKERPRY
                     VVNHVINKFSSFGVTDTYSIKLETDTSKGFIRINSIDLKASTRGVNTPESWTGNYFKG
                     VPLTIKAIPEDGYVFDRWEGTDETSDTIVITPAGDISLKAIFKKAGSTDCKISGYVNP
                     DLSSTAADIKSNFKVEVSGLDVSAFTDEDGYFELSVPQNDTGYVFKISKTNYLTRKVK
                     IDKVSQDMALSSQESPLILWAGDIEIDGHSDGAINMGDIVEMIRVFNSIPSDTEYNGD
                     MDFNKDNAINLKDILIVIKHFNTTSNYYN"
BASE COUNT          796 a          396 c          510 g          661 t
ORIGIN      
        1 gagagcccaa tgtattatcc atgattaaca caggagatta ttattgggat cctcctttag
       61 gagaagtatt caagggtaca acggtaaaag ctgttgctgt aaggtcagac ggtaaagtga
      121 gccgaattgt gacctgctcc tattttgtag atcccgatat gaatacaagg tacagtctcc
      181 ctgtcatttc aattgtaacc gatgaggcaa acttatttga caaaaacacc ggaatatatt
      241 taaacagtaa taaaagcggg gctgattggg aaagacctgc tcatattgaa ttctttgaag
      301 aggatgggac tctcggtttt tcccactatt gcggaataag gcttcatgga ggaggttcaa
      361 aagggttcgg tcaaaaaagt cttcgattat acgcagaccg gggatacgat tataaggaga
      421 aaatcagcta taatatattc cccggattaa aagacaaagt gactggaaaa agtataaccg
      481 attttaagcg acttatattg cgaaattccg gaaatgactg gtcaaacagc atgttcaggg
      541 atgggctgat gcacaaactg gtttctcatt taaaacttga tacacaagca tataggccat
      601 cagtagtatt tattaacggt gaatattggg gaatacataa catacgcgaa cggtatgaca
      661 atatatattt tgcatcccat tataatctgg acaaggacag ggtcgcactg ctcgaagtta
      721 catattttgg aactctcacc gtaaatgaag gaacagatga ggatgcacaa gcttatacca
      781 atgatattat caactttctt aaatcaaacg atattaccca aaaagataat tatgagtaca
      841 taaagacgaa aatggatatg gataatttta ttgattatca ggttgcgaat atttactttg
      901 ccaatggcga ctggcctcag aacaatgtta gcatgtggaa atacaagacc gaggacggtt
      961 tgtaccaccc ggaggcaccc tatgggcaag acggtagatg gagatggatc ataaaagata
     1021 cggattttgg cttttgtgga ccaatgatgg cttctagcgg tattactcat gatactttga
     1081 gtcatgcttc ggaaaatgcc caaaatgaat gggcggtatt tctttttaaa acactactga
     1141 agaattctga gttcagaaat gcgtttatca atagaatggc cgattatctt aatacctgct
     1201 ttgagcctga attagtaaca gatacgatag atgaaatgaa gaatgtcata gaatcttcaa
     1261 taccggaaca taatgcccgt tggcaagcta ttttcgattg ggacggtgag gtagatttaa
     1321 tgcggacctt ttcaaaagaa cgtcccaggt atgtggttaa tcacgtaatt aataaattca
     1381 gcagctttgg tgttacagat acttattcga taaagctgga aaccgatact tcaaagggtt
     1441 ttatccgaat taattccatt gatcttaaag cttcaacccg cggagtgaac acaccggaat
     1501 catggacagg caactatttc aaaggtgtgc ctttgacaat taaggctatt cctgaagacg
     1561 gatatgtttt tgaccggtgg gaaggaaccg atgaaacctc cgataccata gttataacgc
     1621 cagcaggaga tataagcttg aaggcgatat ttaaaaaagc cggcagtaca gattgtaaaa
     1681 tttcaggata tgtaaatccc gatttatcct cgacggctgc cgatataaaa tcaaatttca
     1741 aggtagaagt ttcaggactg gatgtttcgg cttttacgga cgaggatgga tattttgaat
     1801 tgtctgttcc ccaaaacgat acaggttatg tttttaaaat aagcaaaacc aattatctta
     1861 cgagaaaagt taaaatagat aaagtttcac aggatatggc tttgtcttct caggaatcac
     1921 cgttgatttt atgggccgga gatattgaaa tagatggaca ttcagacggt gccataaata
     1981 tgggagacat tgtagaaatg ataagagtat tcaattccat tccgagtgat acagaataca
     2041 atggggatat ggattttaac aaagataacg caattaactt aaaagacatt cttattgtaa
     2101 taaaacattt taatactaca tccaattact acaactaaaa cagatgttat tgagtgcaat
     2161 aaataaatga gaatagaaat atacgccgga tcaaaatatg tataataaaa taatgcagga
     2221 gagtcgtaat gttgactctc ctgtataatt agcgggattt cacgatctga aatgaccaaa
     2281 aaacgatggg ggcttgatgc cctcatcgtt tttactgtgt gccaggcatg gcactaatct
     2341 agctagtgaa agtctagcca cgg
//
LOCUS       DAOOJC010000385         2361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_35430_length_2361_cov_3.728534, whole genome shotgun sequence.
ACCESSION   DAOOJC010000385 DAOOJC010000000
VERSION     DAOOJC010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35430_length_2361_cov_3.728534"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..>2361
                     /locus_tag="PLA01_09300"
     CDS             5..>2361
                     /locus_tag="PLA01_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HPD01527.1"
                     /translation="MLIIFIQLFLFSAVNAQGENLVKNPGFEEGDPHSPNFWSTHSWN
                     KEKGRSEFFLDETQFHSGSRSACIVNNNENDSRYKQAIKVKGNTYYRISCWVKTENVK
                     SDIKGANLSLEGSTDTSRDVRGTSGNWEYIELYGKSSPNQDTFTLTVGLGGHGNINTG
                     KAWFDDIEVIELDSLPPGKTAVSLDPGYTGPDSDGKTGGSSTNNGYIIFASILFIAAI
                     LSFIIFTFFKDKIPGLKSKFSSGTHNEGSNMNNESSGWIKVRYDKTDFIIMGIMTFIY
                     LCMALYNLGDLKVPTTSWEPTMPDETFTVDLGKDAQLSRIYYYCGLGSTKKIDCKLRV
                     EYLDETQGFKHLATIDKENIFIWKYIDTAPVKTRQLRFIVDVSGGSINEIAVVEQGST
                     EPIKSISIIDRQIEEKSKGRLENLFDEQEKFAFKPSYMNGMYFDEIYHARAAFEYVHR
                     MTPSETTHPPLGKLFLAIGILIFGMVPFGWRIIGTLFGVAMVPVMYAFGKKIFRDRLY
                     AFCSAFLMMFDFMHFSQTRIATIDSYVTLFVILMYYYMYDYFINKSYLVELKESLKPL
                     FLCGLFFGLGASSKWIAFYGSIGLALLFLVNKTAEFIEYKKLSKKRTSKSTWVEKYPR
                     SLAVTLGATVLFLAIIPAIIYIFSYIPWMLTPNNKSGIGIFWDNSLDMLKFHSGLQST
                     HPYQSAWWEWPVMVKPMAFYFGKDLEPGMASKIFTMGNPAVWWIGLLALLLVSIWALS
                     KLNKNIVVLFTLSVFSFGYIALPKTVITTIFKNVNAALWQLCKKIS"
BASE COUNT          697 a          475 c          496 g          693 t
ORIGIN      
        1 ctttatattg attattttca tacagctttt tcttttttct gctgtaaatg cacagggaga
       61 aaaccttgta aaaaatccgg gatttgaaga aggtgatccc catagcccga acttttggtc
      121 cactcacagc tggaacaagg aaaaaggcag atcggaattc tttcttgatg aaactcagtt
      181 tcactccggc agcaggagtg cctgtatagt taacaacaat gaaaatgatt ccagatacaa
      241 acaagctatc aaagttaagg gaaatactta ttacagaatt tcatgctggg taaaaaccga
      301 aaatgtcaaa tcagatatta aaggtgccaa cctttccctt gaaggctcta cagatacctc
      361 aagggatgtt cgcgggacaa gcggcaattg ggaatacatt gagctttacg gcaaatccag
      421 ccccaatcag gatactttca ctcttactgt gggcttggga ggccacggca atattaatac
      481 gggtaaggcc tggtttgatg atattgaagt cattgaactg gattccctgc ctccggggaa
      541 aactgctgta agccttgatc cgggctacac aggtccggac agtgacggca aaaccggcgg
      601 cagcagcaca aataacggtt atattatctt tgcttcaatt ctcttcattg ctgctatact
      661 atcttttatt atcttcacgt ttttcaaaga caaaataccc ggtcttaaat caaaattcag
      721 cagcggtacc cacaatgaag gcagcaatat gaataatgag tcatctgggt ggataaaagt
      781 cagatatgat aaaaccgact tcattattat gggtattatg acttttatct atctttgcat
      841 ggcattatat aatctgggtg atttgaaggt tccgactact tcatgggaac ccacgatgcc
      901 cgacgaaacc tttaccgtag atctgggaaa agatgctcag ttgtccagaa tttattacta
      961 ttgcggactg ggaagtacca aaaaaatcga ttgcaagtta agggtcgagt accttgatga
     1021 gacacaaggc tttaagcatc ttgccaccat cgataaagaa aatatattta tatggaaata
     1081 tattgacact gctccggtaa agacacgtca actcaggttc atagtggatg tctccggagg
     1141 ttctatcaat gaaatcgctg tcgttgaaca gggcagcaca gaaccgataa aaagcataag
     1201 catcattgac cggcagatag aggaaaaaag taaaggccgt ttggagaacc tgtttgatga
     1261 acaggaaaaa tttgctttta agccttctta tatgaacggt atgtactttg atgaaatata
     1321 ccacgccaga gctgccttcg agtatgttca tcggatgact ccttcagaga ctacacatcc
     1381 gcctctcggc aagcttttcc tggcaatagg aatccttata ttcggaatgg ttcctttcgg
     1441 ttggcgaatt atcggaacac tgttcggtgt tgctatggtt ccggttatgt acgctttcgg
     1501 taagaaaatc tttcgtgaca ggctttatgc cttttgttca gcctttctga tgatgtttga
     1561 cttcatgcat ttcagccaga cacgaatagc aaccatagac agctacgtta cattatttgt
     1621 catattgatg tactactata tgtatgatta ttttataaac aagtcctatc ttgtggaact
     1681 aaaagagtct ttaaaacctt tattcctttg cggactgttt ttcggtctgg gggcatcgag
     1741 caagtggata gctttctacg gatccattgg gcttgccttg ttgttcttgg tcaataaaac
     1801 tgcagagttt attgaatata aaaagctgtc aaaaaagagg acctccaaaa gcacctgggt
     1861 tgagaaatac cctcgaagcc tggccgtcac attaggtgcg acagtgctgt tcctcgcaat
     1921 aattcccgca ataatatata tattctcata tattccatgg atgctgaccc caaataacaa
     1981 aagcggtatc gggatattct gggataattc tttggatatg ctaaagttcc attccggact
     2041 tcaatccacg cacccatacc aatctgcatg gtgggagtgg cctgtaatgg taaaacctat
     2101 ggcgttttat ttcggaaagg atcttgaacc cggaatggct tcaaagatct ttacaatggg
     2161 taatcccgca gtctggtgga ttggactttt agcactatta ttagtcagta tatgggcact
     2221 ttcaaagctt aacaaaaaca ttgttgtctt atttactttg tcggtatttt ctttcggata
     2281 tattgcattg cccaagacgg taataaccac tattttcaag aacgtaaacg cagcactctg
     2341 gcagctttgc aaaaaaatat c
//
LOCUS       DAOOJC010000386         2358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_35498_length_2358_cov_4.754668, whole genome shotgun sequence.
ACCESSION   DAOOJC010000386 DAOOJC010000000
VERSION     DAOOJC010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35498_length_2358_cov_4.754668"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..1565
                     /locus_tag="PLA01_09305"
     CDS             309..1565
                     /locus_tag="PLA01_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD01528.1"
                     /translation="MLSQINSVIFCTTQIMQVVIFIAGCYFFGISIFGWRKRKEKDPG
                     ECLPQKKFALVVAAHNEETVIAHIVDSLNRQNYPGNLFDVFVIADNCTDRTAEIAEEH
                     GAKVFKRFNNSARGKGHAIEWMFAQIFNMEEKYDAICVFDADNLVTSNYLLEMNRQLC
                     EGHKVIQGYIDSKNPFDSWITCSYSIAFWLSNRLFQLPRYYLGLSCGLCGTGFCVDVD
                     VLKEIGWGATCLTEDLEFTMKLALSNYKVAWANNAVVYDEKPITLKQSWNQRKRWMQG
                     HADCASRFLGPLFRKAFREGNLIAFDCAVYLFQPIRLVFIGLITIMMWVQTIFPTSPF
                     YNLRYVFPTQVWYVFVTLQFLYGPLVVLSERKFNLKALIGFLIYPFYCITWIPITIQG
                     FLSKNNKDWSHTRHSREISILDLEKA"
     gene            complement(1681..>2358)
                     /locus_tag="PLA01_09310"
     CDS             complement(1681..>2358)
                     /locus_tag="PLA01_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD01529.1"
                     /translation="SRLVGSPPGYVGYEEGGQLTEKVRRRPYSVLLFDEIEKAHPDVF
                     NILLQILEDGRLTDSQGRTVDFRNTVIIMTSNVGARLITEPKRMGFSTGVENTERNYN
                     DMKNNVMGELKKSFRPEFLNRIDEIIVFHPLEEEHLKKIVELMINNLAKRLEQSSINI
                     EVSEDAIALLAKKGTDQIYGARPLRRAIQSMVEDKLAEEMLEGRVKAGDKVLVQRKDD
                     ELVLNKE"
BASE COUNT          682 a          479 c          465 g          732 t
ORIGIN      
        1 taatgttaaa atccacttcg tgaaatcccg ttaattaatt atggatcgag acatatgtaa
       61 ataaacacct tgaaaatgaa attagatatt ggcaatataa tatatttaat tatagcatat
      121 tttattattt ttttatcata aatatttttc cttattaagt acaaaataat atagaaataa
      181 atgaagccgg aaaaagaaac tcaacctaat aaatacagtg tcataaattc tggatagacg
      241 tgaagtgtta tttgaaatca gcttttaaaa cggtgtaaaa aaacataata atattttgga
      301 ggaatcagat gttatcgcaa atcaacagtg tgatattttg cacaacacaa attatgcagg
      361 ttgtaatttt tatagcaggg tgctactttt tcgggatatc catctttggc tggcgaaaga
      421 gaaaagagaa ggaccctgga gaatgtcttc ctcaaaagaa atttgctctg gttgtagccg
      481 cccataatga agaaacggtt attgcccaca tagttgacag tcttaacagg cagaactatc
      541 cgggcaatct ttttgatgtt tttgttattg ccgacaactg cacggataga accgcagaaa
      601 tagcagaaga acacggagca aaagtattca agaggttcaa taacagtgcc cggggtaagg
      661 gacatgccat agaatggatg tttgcacaaa tattcaatat ggaagaaaag tacgatgcca
      721 tttgcgtttt tgatgccgac aatcttgtca catcgaatta cttactggaa atgaacaggc
      781 aactctgcga aggccataag gttatacagg gctatatcga cagcaaaaac ccctttgatt
      841 cctggattac ctgctcgtat tccattgctt tttggctttc gaacaggtta ttccagcttc
      901 ccagatacta cctgggactg agttgcggcc tttgcggtac aggcttttgt gttgatgttg
      961 atgttttaaa ggaaataggt tggggagcaa cttgtcttac cgaggatctg gaatttacaa
     1021 tgaagcttgc cctcagcaat tacaaggttg catgggcaaa taatgctgtt gtgtatgacg
     1081 aaaaacccat caccttgaaa caatcatgga accagcgtaa aagatggatg caaggtcacg
     1141 ctgactgtgc aagccgtttt ttaggtcccc ttttccgaaa agcttttagg gaaggaaatt
     1201 taatagcttt tgactgtgcg gtatatttgt ttcaaccgat aaggttggtg tttatagggc
     1261 tgattacaat aatgatgtgg gttcaaacca tatttcccac atcaccattt tacaatctga
     1321 ggtatgtttt ccccacccaa gtctggtatg tatttgtaac cctgcagttt ttatacggcc
     1381 ctttggttgt gctttctgaa aggaaattca atttaaaggc actgatcggt tttctgattt
     1441 atccgtttta ctgcataaca tggattccta ttacgataca gggcttttta agcaagaaca
     1501 ataaagactg gagtcatacc cgtcactcaa gggaaataag catattggat cttgaaaaag
     1561 cataaggggg gagaagagct agccgcctgt aaatactcct tgtgggtcga gcaaaacccg
     1621 cttcgcaaaa acggatgctc tcccattcct cgccgtatta acaggcggct gcaacttgct
     1681 tcactctttg ttaagtacca gttcatcgtc ctttctttga acaagaacct tgtcaccagc
     1741 tttcactctg ccttcgagca tttcctccgc cagtttatcc tctaccatgc tctgtattgc
     1801 cctcctcaaa ggccttgcac cgtaaatctg gtcggttcct ttctttgcca gaagagcaat
     1861 ggcatcttca gatacctcaa tattgataga gctttgttca agtcttttcg caaggttgtt
     1921 tatcataagc tccactatct ttttaagatg ctcctcttct aacggatgga acactataat
     1981 ctcatctatt ctgtttaaaa actcaggtct gaagctcttt ttaagctctc ccataacatt
     2041 gtttttcata tcgttgtaat ttctttcggt gttttcaacc ccggtactga agcccatacg
     2101 ttttggctcg gtaataagtc ttgcacctac gtttgaagtc ataattataa cagtatttct
     2161 gaagtctacc gtccgccctt gggaatccgt cagtcttccg tcctcaagaa tctgcagcaa
     2221 tatattgaat acatcgggat gggctttttc aatttcgtca aacagaagta ccgaataggg
     2281 tcttcttctg accttctccg taagctgacc gccttcttcg tatccaacat atcctggagg
     2341 tgaacccaca agccttga
//
LOCUS       DAOOJC010000387         2352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_35639_length_2352_cov_3.090553, whole genome shotgun sequence.
ACCESSION   DAOOJC010000387 DAOOJC010000000
VERSION     DAOOJC010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35639_length_2352_cov_3.090553"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /gene="thiF"
                     /locus_tag="PLA01_09315"
     CDS             complement(<1..464)
                     /gene="thiF"
                     /locus_tag="PLA01_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HPD01530.1"
                     /translation="MNEFLKGLVSYIGEERLERLKKVKVGIAGAGGLGSNCAISLVRS
                     GFHKFKIVDFDVVEPSNLNRQFFFLNQLGMPKVLALRENLKRINPDIKIEAIQQKVQK
                     SNAKELFDDCDVVVEAFDRVPCKTMMVEEFISSGKFFVCASGLAGWGNSDEI"
     gene            complement(547..1650)
                     /gene="thiH"
                     /locus_tag="PLA01_09320"
     CDS             complement(547..1650)
                     /gene="thiH"
                     /locus_tag="PLA01_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HPD01531.1"
                     /translation="MSFYERYIKYKNFDFEAFFDRVCEQDIINIINKDRITELEFLML
                     LSKKALKYLEPMAQRARKLTIQNFGKVIFLYTPMYLANYCVNQCVYCGFNITNKITRR
                     KLTLEEVEKEAQVISSTGLRHILILTGESRLKSPVEYIRQCVEVLKKYFRSISIEIYP
                     LGQMEYAELIEAGVDGLTIYQEVYNEEVYEKLHIKGPKRNYMYRLEAPERACRASMRS
                     VNIGALLGFDDWRKEAFFTGIHADYLQSRYPDTEISISLPRIRPHCGSFKPAYAVEDS
                     DLVQIMLAQRLFMPRAGITVSTREGQGFRDNLIGLGVTKMSAGSSTEVGGHTLEDKSE
                     GQFDINDRRGVEEIKQMIYKKGYQPVFKDWQAI"
     gene            complement(1787..>2352)
                     /locus_tag="PLA01_09325"
     CDS             complement(1787..>2352)
                     /locus_tag="PLA01_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HPD01532.1"
                     /translation="NTSGARNAEEAVRIARLARAAGCGNFVKIEVISDNRYLLPDNRE
                     TVKATEILVKEGFVVLPYMNPDLMDAKRLRDAGAAAVMPLGAPIGTNKGLKTRELVKI
                     LIDEIDIPVIVDAGIGKPFEACEAMEIGAAAVLVNTAIATAGDPVEMAGAFARAVEAG
                     RKAFLSGTGAVRDYAEASSPLTGFLGA"
BASE COUNT          590 a          658 c          402 g          702 t
ORIGIN      
        1 tttatctcat cgctgtttcc ccatccggcc aatcccgatg cacagacaaa aaacttcccc
       61 gatgatataa actcttcgac catcatggtt ttgcatggca cccggtcaaa agcctccacc
      121 acaacatcac agtcatcaaa cagctctttg gcattactct tttgcacttt ttgctgtatg
      181 gcttctattt tgatgtccgg gttaatcctt ttgaggtttt ccctaagtgc cagaaccttg
      241 ggcattccca gctgattaag gaagaaaaac tgccggttca ggtttgatgg ttccaccaca
      301 tcaaaatcga ctattttgaa tttatgaaat ccgcttctga caaggcttat agcacagttt
      361 gaacccagcc ctcctgcacc ggcaatcccc accttaacct tttttagcct ctccagcctc
      421 tcttcaccga tgtaggacac cagccctttt aaaaactcat tcatataccc ctccacaaat
      481 actatattat ctggcagtct tttgtattgt gaccgtaaaa gagtccaatg ctttcaccat
      541 aaacaactat attgcctgcc agtccttaaa aaccggttga taacccttct tgtaaatcat
      601 ttgctttatt tcttcaactc ccctccggtc attgatgtca aattgccctt cactcttatc
      661 ttccagggta tgtcctccta cctcggtact tgacccggca gacatttttg tgacacccag
      721 accaatgagg ttatccctga agccttgacc ctccctcgtg gaaaccgtta ttcccgctct
      781 tggcataaaa agcctctggg caagcattat ctgaaccaag tcactgtcct cgacggcata
      841 tgcaggcttg aaacttccgc aatggggcct tattctcgga agggatatgc ttatctccgt
      901 atccgggtac cggctctgaa gatagtccgc atgaattccc gtaaaaaatg cttccttcct
      961 ccagtcatca aaccccaaaa gtgcacctat gtttacactt ctcattgatg cccgacaagc
     1021 ccgttccggt gcctcaagcc tgtacatgta atttctcttt ggccccttta tgtgaagctt
     1081 ttcgtacacc tcttcgttgt atacctcctg gtaaatcgta aggccgtcaa caccggcttc
     1141 tattagttct gcatactcca tctgacccag aggatatatt tctattgata tggacctgaa
     1201 atactttttt aagacctcga cacattgtct aatgtattcc accggacttt taagcctcga
     1261 ttctcccgta agaattaaaa tatgtctaag accggtggac gatatgacct gtgcctcctt
     1321 ttccacctct tccaaggtta gctttcttct tgtgatctta ttggttatat taaatccgca
     1381 atacacgcat tgattcacac agtaattggc caaatacatc ggtgtgtata agaatataac
     1441 tttgccgaaa ttctgtattg tcagcttcct tgccctttgt gccattggct ccaagtactt
     1501 caaagccttt ttggacagaa gcatcaaaaa ttcaagctcc gtaattctgt ctttgttgat
     1561 aatgtttata atgtcctgct cacaaaccct atcaaaaaaa gcttcaaaat cgaaattttt
     1621 gtactttatg tatctttcat aaaagctcat aattcactcc ccatccctta aggttaagta
     1681 aaatattccg gaaaccggag ttttgacggc tagccgcgtg accccacaat gtttctttca
     1741 tattttcttc acaagtttac tccaccgcta acaatgaatc actcttctat gctcccagaa
     1801 aacctgtcag aggagaggaa gcctcggcat aatcccttac cgcaccggtt ccggaaagga
     1861 aagcctttct tccggcctca actgctctag caaaggcccc agccatttct accgggtctc
     1921 ctgcggtagc aatcgcggta ttgaccagaa cggctgcagc cccaatttcc atagcctcac
     1981 atgcctcgaa aggctttccg atacccgcat caacaatcac aggtatatca atttcatcaa
     2041 tcaggatttt aaccaattcc ctggtcttaa gccctttatt cgtaccaata ggagcaccca
     2101 acggcattac cgccgccgca cctgcatccc ttagcctctt ggcatccata aggtcagggt
     2161 tcatataagg cagcaccaca aatccttcct ttacaagtat ctccgttgcc ttaacggttt
     2221 cccggttgtc cggaagcagg taccggttat ccgatatcac ctctattttt acaaagttac
     2281 cgcaacctgc ggctcttgca agtcttgcaa tccttacagc ctcctcggca tttctggccc
     2341 ctgaggtatt gg
//
LOCUS       DAOOJC010000388         2340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_35881_length_2340_cov_3.548359, whole genome shotgun sequence.
ACCESSION   DAOOJC010000388 DAOOJC010000000
VERSION     DAOOJC010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35881_length_2340_cov_3.548359"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="PLA01_09330"
     CDS             complement(<1..956)
                     /locus_tag="PLA01_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPD01533.1"
                     /translation="MICFNCGIKIHSNEVKVCQLCGVRFEKTCLSCNSPNPLMGKYCF
                     NCGAKLESLDTQSSVQNFDTLSESRRNVAVIFADVSGFTALSEKLDPEEVREIINDCF
                     DYITRPVYELEGTIDKYIGDCVMVLFGAKYIHSDDAKRAVVCAMRMMNLIEEFSKERL
                     SSKGLSLTLSIGINYGLVVTGSVGNTFDKDYTVMGDIVNTAQRLQSNAESGSIFVSES
                     VYVETKGIIKYTKPVEIKVKNREKTVRCYLPIEINAEHDYDDRTPFIGREKELGQLVS
                     IYNYAINTNTKCISIIGEAGVGKTRLLEEFSSNLAMDIKKVWV"
     gene            1127..>2340
                     /locus_tag="PLA01_09335"
     CDS             1127..>2340
                     /locus_tag="PLA01_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPD01534.1"
                     /translation="MLNATAFLLAGSNVCEYQKFIDLLRTNIVQINTYSDSDALLSSI
                     NSFSLKNTKNPSTAVIFIEYTLFRDISSDIKVLLEKLDCILVLFGSNDLELNTDLDIV
                     FEYINTKHFNSAFFINKLKREIDSKSEISFLRNEAKEFYEIGKSLSSEKDTVKLLDMI
                     VTSSLKLTSSDAATIYLVIDKDTYNWTSIKNNDYRYKSLKFVIAKNLSMDINLETFIS
                     PITKESIFGYTVITGETVRVDDAYNLSPELDYRHNNSFDLHTGYVTKSILATPIKDHQ
                     GSTLGVIQLINKRKFPDEKIDYKSEDALSKITSYDYKDELIMNSLAGHAAVALENNHL
                     YREMKNLLDGYKRQNSELEALSKKILIAHEEERKRIAREIHDGPAQSVVNLSLRVELA
                     KKLLQNSMYDKCV"
BASE COUNT          771 a          500 c          385 g          684 t
ORIGIN      
        1 acccatacct tcttaatatc cattgcgaga ttagaggaaa actcctctaa aagccgcgtc
       61 tttccaaccc ctgcctctcc aattatacta atgcattttg tgtttgtatt gatggcataa
      121 ttatatatgg atacaagctg ccccagttcc ttttcccttc cgatgaatgg cgttctgtcg
      181 tcataatcgt gctccgcatt gatttcaata ggcaagtaac atcttacagt cttttctctg
      241 tttttaacct ttatctcaac aggctttgtg tacttgatta tgccctttgt ttccacatat
      301 accgactctg acacaaaaat ggatccgctt tccgcattgg actgcagcct ctgggcagta
      361 ttaacaatat ctcccatcac cgtataatcc ttgtcgaaag tatttcccac actacctgta
      421 accaccagcc catagtttat gccaatggaa agagtcaaag acaaaccttt agacgaaagc
      481 ctttcctttg aaaattcctc tataagattc atcattctca tggcacagac tacagctctt
      541 ttggcatcat cgctatggat atactttgct ccgaaaagta ccattacaca gtcaccgata
      601 tacttgtcta tagtaccctc cagctcataa acaggcctgg taatataatc aaagcaatca
      661 tttatgatct ctcttacttc ctcaggatca agcttttctg aaagagccgt aaacccggaa
      721 acatcagcaa aaataacagc cacattcctc ctgctttcag aaagggtgtc aaagttctga
      781 accgagcttt gtgtatcaag agattccaat ttagcaccgc agttaaagca atacttaccc
      841 atgagaggat tcggagaatt acaggaaaga catgttttct caaacctcac gccacaaagc
      901 tgacatacct tgacttcatt gctgtgaatt tttataccgc aattaaaaca aatcatttgc
      961 tttcccccgg agaaatttat atactcaaat aacgaacaaa caaaagtttt tttgaattaa
     1021 agacaataat attgtataat atatatatcg attattctgt tttcattttt aacgctttcg
     1081 ctatggttaa ataagtcaat aagtcacatg ataaggggta aataaaatgt tgaatgcaac
     1141 cgcattttta ttggcaggtt caaatgtttg cgaataccag aagtttattg acttgcttag
     1201 aaccaatatt gttcaaataa acacctattc agacagtgat gctttattaa gcagtattaa
     1261 cagtttttct ttaaaaaaca caaaaaaccc ttccaccgcc gttatattta tcgaatacac
     1321 cttattccgg gatatttctt ccgacattaa ggttcttttg gaaaaattag actgcatcct
     1381 cgttttattc ggttccaatg atttggaatt aaacacagat ttggatattg tatttgaata
     1441 tataaataca aagcatttca acagtgcttt tttcataaat aagttaaaaa gagagattga
     1501 cagcaaaagc gaaatatcct tcctgcgaaa tgaggccaag gaattttatg aaataggcaa
     1561 atccctttct tcagaaaagg atacagtaaa gcttctagat atgatagtaa ccagcagctt
     1621 gaaacttact tcctccgatg cggcaacaat ctatcttgta atcgacaagg acacctacaa
     1681 ctggacgtcc ataaaaaaca acgactatag atacaaaagc ttaaaattcg ttattgcaaa
     1741 aaacttaagt atggacataa accttgaaac attcatttct cccattacaa aagagagtat
     1801 ctttggttac acagttatta caggtgaaac cgtaagggtt gacgatgcct acaatctatc
     1861 ccctgagctt gattaccgcc ataacaacag ctttgatctg cacacgggat atgtgacaaa
     1921 atccattctt gccacaccga taaaagacca ccaaggcagt accttaggtg tcatccagtt
     1981 aattaataag aggaaattcc cggatgaaaa gatagattat aaaagtgagg atgcgttaag
     2041 caaaataacc tcctatgatt ataaagacga attgataatg aattcccttg cgggtcatgc
     2101 agccgttgcc ctcgaaaaca accatctgta cagagagatg aaaaaccttc tcgatggcta
     2161 taaaaggcag aacagcgaac ttgaggcatt aagcaaaaaa attctcattg cccatgaaga
     2221 ggaaaggaaa agaattgccc gtgagattca tgacggcccc gctcaaagcg ttgtaaacct
     2281 ttccttgagg gttgagcttg ccaaaaagct ccttcaaaat tccatgtacg ataaatgtgt
//
LOCUS       DAOOJC010000389         2335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_35979_length_2335_cov_3.475000, whole genome shotgun sequence.
ACCESSION   DAOOJC010000389 DAOOJC010000000
VERSION     DAOOJC010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35979_length_2335_cov_3.475000"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="PLA01_09340"
     CDS             complement(<1..143)
                     /locus_tag="PLA01_09340"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPD01535.1"
                     /translation="MADIFSNVLEIRWEMVLMWVIGGVLIWLAIAKDMEPTLLLPMGF
                     GAIL"
     gene            290..955
                     /gene="deoC"
                     /locus_tag="PLA01_09345"
     CDS             290..955
                     /gene="deoC"
                     /locus_tag="PLA01_09345"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPD01536.1"
                     /translation="MNIENILSKVDHTLLKQTAKWEDIKTICEDAIKYNCASACIPPY
                     FVKQAKDCFKNNLKICTVIGFPNGYSTTATKCFETARAVSDGADEIDMVLNINMLKDK
                     LYDEILTEINQVKKACSGKLLKVIIETCLLTEEEKIKMCEIISKSDADYIKTSTGFST
                     AGATREDIMLFKEHIKNGKLIKAAGGIRSLNDANDFISLGSSRLGTSSIVRIVKDEEN
                     TSY"
     gene            1073..2149
                     /locus_tag="PLA01_09350"
     CDS             1073..2149
                     /locus_tag="PLA01_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPD01537.1"
                     /translation="MKKLFSIVLVVVLLLSMLSLAGCQAKTYELAMITDIGDIDDKSF
                     NQGTWEGLRDYAVEHKITHKYYKPTEASTDAYIAAIDLAVSSGAKVVVTPGFLFEEAI
                     YIAQDKYPEIMFILLDGVPHDANYENFKTGPNVFPVVYAEEHCGYLAGYAAVVDGYRN
                     LGYQGGMAVPAVCRYGFGYVQGANDAARDLGLAKGSIKIMYNYSGDFADTPASQTRAA
                     GWYQAGTEVIFAAGGKVGNSVMSAAEAANKAVIGVDVDQSAESPTVISSAMKGLATMV
                     QYGLKGFYAGKFPGGEAVTVGAESNAVSLAPFSTSKWKTFSQADYDALFAKLANGTIV
                     PEKDLDRTNPTELKLDYVKLTFVE"
BASE COUNT          759 a          444 c          491 g          641 t
ORIGIN      
        1 agaattgccc cgaaccccat aggcagaagc agtgtgggtt ccatgtcttt ggctatagcc
       61 agccaaatca aaacccctcc tatcacccac atgaggacca tctcccatct tatctccagt
      121 acattagaaa aaatatcagc catgattatc tcttcccccg atttttacag gattaagcac
      181 ctgttcatct ttagtatata taatactttt ttcctgccgc ttgcgtcaag tgattagttg
      241 cattaaaccg tttgtatata tatactttac tgggaaagga attgtaaaaa tgaatataga
      301 aaacatatta tccaaagtgg accatactct tcttaaacag acggctaagt gggaagatat
      361 taaaacgatt tgtgaagatg cgattaaata taattgtgcg tcggcctgta ttcctccgta
      421 ctttgttaag caggcaaaag attgttttaa gaataacctt aaaatctgta cggttatagg
      481 ttttcccaac ggatacagca ccacggcaac caagtgtttt gaaactgcac gggctgtaag
      541 cgacggagct gacgaaatag atatggttct caacataaac atgctcaaag acaagcttta
      601 cgatgaaata ctgaccgaaa ttaatcaggt taaaaaagcc tgttcgggaa aacttcttaa
      661 agttataatc gaaacctgtc ttctgaccga agaggaaaag ataaagatgt gcgaaataat
      721 atcaaaaagc gacgccgact atataaagac atcaacgggc ttttccacag caggcgccac
      781 gagagaagac attatgcttt ttaaggaaca tattaaaaac ggcaagctca taaaagccgc
      841 cggaggcata aggtctttaa acgatgcaaa tgattttatc agtctcggct cttcccgact
      901 gggcacatca agtatagtaa gaattgttaa ggatgaggaa aatacaagtt attgaaatgt
      961 taaagaaatg tgtcaagaat gtgaactttt tgctttttcc cccttgttta taaactataa
     1021 atctgatagt atttatatgt gaatttcatt atatcaaaaa aggagtataa ttatgaaaaa
     1081 actatttagt atcgtattgg tagtggtgct tttgctttct atgctgtcct tagcaggctg
     1141 ccaagctaaa acctatgagc tggcaatgat taccgacata ggagacattg acgacaagtc
     1201 ctttaaccag ggaacatggg aaggattgag ggactatgcg gtagagcata aaattacgca
     1261 taagtattac aagcccaccg aagcttccac cgatgcatat attgcggcta ttgatcttgc
     1321 agtatcatcc ggagctaaag ttgtcgtaac accgggattc cttttcgaag aagctatata
     1381 catagctcaa gataaatatc cggaaataat gtttattctt cttgacggag taccgcatga
     1441 tgcaaattac gagaacttca aaaccggtcc caatgttttc cctgttgtat atgcggaaga
     1501 gcattgcggt taccttgcag gctatgctgc tgtagtagac ggatacagaa acttgggtta
     1561 tcagggcgga atggccgttc cggcagtttg cagatatgga ttcggatatg tacagggtgc
     1621 taatgatgca gccagagatt tgggccttgc aaaaggatca attaaaataa tgtacaacta
     1681 ttccggagac tttgccgata caccggcatc acagacaaga gctgcaggct ggtatcaggc
     1741 aggaaccgaa gttatcttcg cagcaggcgg aaaagtagga aactccgtta tgtcggcagc
     1801 ggaagcagcc aataaagcgg ttataggtgt tgacgttgac caatcggcgg aatcgcctac
     1861 ggttatctct tcggcaatga agggattggc aacaatggtt cagtacggat taaaagggtt
     1921 ctatgcagga aagttcccgg gcggagaagc cgtaacggtc ggtgcagaat ccaatgctgt
     1981 aagcctggca ccgttctcaa cttcaaaatg gaaaacattc tcacaggccg attatgacgc
     2041 attatttgca aaattggcta acggaactat agttccggaa aaagatctcg acagaacaaa
     2101 tcccacggaa ctcaaactcg attatgttaa attaactttt gtagagtaag agtaaaacaa
     2161 taaagaaaca aaaactgccg tataaaagcg gcagtttttt atcttgtaat actatatatt
     2221 tctgtttttt cactttagcg gttattccgt atttattatt cttttcaaat attatataat
     2281 aaagtaagtt aaaaacactt aaagccaagg agcaaattta tggattacat catcg
//
LOCUS       DAOOJC010000390         2332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_36049_length_2332_cov_3.695652, whole genome shotgun sequence.
ACCESSION   DAOOJC010000390 DAOOJC010000000
VERSION     DAOOJC010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36049_length_2332_cov_3.695652"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PLA01_09355"
     CDS             complement(<1..525)
                     /locus_tag="PLA01_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPD01538.1"
                     /translation="MSNSFIVALCQMEVVDDKEKNIKKAADMIEESARNGVKLVVLPE
                     MFNCPYDNSQFPGYAETAGTGLTISAVSKAARENSVYVVAGSIPEKDNNKIYNTCFIF
                     DDNGRIIGRHRKIHLFDIDIPDKIVFKESDVLTPGNDITVVDTAFCRIGVAICYDVRF
                     PEIFRKMVLMGAELI"
     gene            829..1056
                     /locus_tag="PLA01_09360"
     CDS             829..1056
                     /locus_tag="PLA01_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HPD01539.1"
                     /translation="MEQPACHANMIQPYQAPSTIYAATVHCIPMKLAHAYVPFQCIGC
                     LFPPMRGLDAGTIFPELARPYGVDPEYTIDE"
     gene            1068..1322
                     /locus_tag="PLA01_09365"
     CDS             1068..1322
                     /locus_tag="PLA01_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HPD01540.1"
                     /translation="MNTNRESLLKEVMAADFTLIDLNLYLNTHPYDQTTIMLFNNCAQ
                     RARMLRNEYERLYGPLTASNSTCKCPWQWISSPWPWEHQS"
     gene            1357..1953
                     /locus_tag="PLA01_09370"
     CDS             1357..1953
                     /locus_tag="PLA01_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HPD01541.1"
                     /translation="MWIYEKKLEYPVKIKTCNPKMAKYIISQYGGPDGELAASLRYLS
                     QRFSMPTNETKAILNDIGTEELAHLEMIGSMVYQLTKGVPADVLEREGLGAYYTDHDH
                     AVYPVNAAGTPFTAAYIQSKGDVIADLHEDLAAEQKARATYENLIRLADDPDVIDPLR
                     FLREREVVHFQRFGEALRLVQDKFADKKFFMSTPIQRA"
BASE COUNT          757 a          472 c          417 g          686 t
ORIGIN      
        1 aataagttcg gcccccataa gaaccatctt tctaaaaatc tccggaaacc tcacatcgta
       61 gcatattgca acaccaatcc tgcaaaaagc cgtatcgact accgttatat cattccccgg
      121 tgtcagtaca tcagactctt taaatacaat tttatcaggt atatcaatat caaaaagatg
      181 tatttttcta tgtcttccta ttattcttcc attgtcatca aaaataaaac atgtgttgta
      241 tattttatta ttatcctttt caggaattga ccctgcaaca acatatacgc tgttttctct
      301 tgcagccttt gaaacagccg aaattgtcaa acctgtcccg gcggtttcgg cataaccggg
      361 gaattgggaa ttgtcatagg gacaattgaa catttcaggc agtaccacaa gctttactcc
      421 gtttcttgcc gactcttcga tcatgtccgc tgcctttttt atgttttttt ctttatcgtc
      481 cacaacctcc atctggcaca aagcaactat aaaagaatta ctcatggcat gctcctcttt
      541 acaataaatt actatataat attacaccca taagattaat ttcgcaaggg tgcagaatgg
      601 agtacgtttt acaaatgcaa atcttttttt cataaattat tcgcacatgg ttaggaaaaa
      661 acataattat agaacacttt acaggtcaat agaatattat gttaatagaa tttaacttcg
      721 tgaaattgca ccaataaaag gttttgccgt catacaataa taagacggca tattattctt
      781 tgttcggagg gaattaaaat gatgcaaaat cataattttt acccaaatat ggagcaacct
      841 gcatgccatg caaacatgat tcagccttac caggctcctt ccacaatata cgctgctact
      901 gttcactgca ttccaatgaa gctggcacat gcatatgttc catttcaatg tataggttgt
      961 ttatttccac ccatgagagg actggatgca ggaacgatat ttcccgagtt agccaggccc
     1021 tatggtgtgg atcctgaata taccattgac gaataggagg caaaaacatg aatacaaata
     1081 gggagagctt gctcaaagag gtcatggctg cggattttac ccttattgat ctcaatctgt
     1141 acctaaacac acacccatat gaccagacga ccatcatgct gttcaacaac tgtgctcaaa
     1201 gagcaaggat gttaagaaat gaatatgaga ggctgtatgg accgttaaca gcctcaaatt
     1261 ccacatgcaa atgtccgtgg caatggataa gcagcccatg gccttgggag catcagtcgt
     1321 aaacatgatt taagataata aaagggaggc aacaaaatgt ggatttatga aaaaaaactc
     1381 gagtatccgg ttaaaatcaa gacttgcaat cctaaaatgg ctaaatatat aatttcacaa
     1441 tatggaggac cggacggaga actcgctgca tctttaagat atcttagtca aagattcagc
     1501 atgcccacaa atgaaacaaa agctatatta aatgatatag gtaccgaaga attggctcac
     1561 cttgaaatga ttggttcaat ggtttaccag ttaaccaaag gtgttccggc agatgtcctt
     1621 gaaagagaag gtcttggggc atactatacc gatcacgacc acgccgtata ccctgtgaat
     1681 gcggctggaa ctccttttac tgctgcttat atacagtcaa agggtgatgt tatagctgat
     1741 ttgcatgagg atcttgcagc agaacaaaag gcaagagcta cttatgaaaa tttgataaga
     1801 cttgctgatg accctgatgt catagacccc ttaagatttt taagagaaag ggaagttgtg
     1861 catttccaaa gatttggaga agccctcagg ttggttcagg ataagttcgc tgataaaaaa
     1921 ttcttcatga gcactccaat tcaaagagcg tgaaaatatg acaccatact ataaatcaag
     1981 caaactacct gtgcccttga gtacttttta tccggattgg ctgttagttg taattgcgtc
     2041 atcgtaatga gaatagtatc gctaaagggg atgattcttt atatcataat tgattgaatc
     2101 atccccttac aacaatattt tttgctcaat aacacttaac cgcattatta ttgcatagca
     2161 tccaattccc attcagatga atgaatctcc ggatcatcga agtttgacct cattattgct
     2221 gcatagcatc cctaccaatt tatcatgttt accaatataa aactttatgt aaattaactg
     2281 atacccttca tgatagttat cgaagatagc tatcaaagca aaaaagtttc aa
//
LOCUS       DAOOJC010000391         2311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_36516_length_2311_cov_3.915337, whole genome shotgun sequence.
ACCESSION   DAOOJC010000391 DAOOJC010000000
VERSION     DAOOJC010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36516_length_2311_cov_3.915337"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..123)
                     /gene="rplW"
                     /locus_tag="PLA01_09375"
     CDS             complement(<1..123)
                     /gene="rplW"
                     /locus_tag="PLA01_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPD01542.1"
                     /translation="MRAAEDILIRPYITEKSNDEIANGKYTFIVDVKATKTEIKQ"
     gene            complement(123..746)
                     /gene="rplD"
                     /locus_tag="PLA01_09380"
     CDS             complement(123..746)
                     /gene="rplD"
                     /locus_tag="PLA01_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPD01543.1"
                     /translation="MPKVDVYDINGKVVGDIELSENIFSVDVNENAIHQVVVNQLANK
                     RQGTQSTKTKSEVRGGGIKPWRQKGTGRARQGSIRSAQWIKGGIVLGPKPRSYSYTLP
                     KKLRRLALKSALSSKVNENEILVLDSLNFDKIKTRQMVDVLKNLNVNETAVIVIADKN
                     ESVQMSARNIPGVKTLLVNTMNVYDIVKHEKFIITRDAVSKVEEVYA"
     gene            complement(768..1406)
                     /gene="rplC"
                     /locus_tag="PLA01_09385"
     CDS             complement(768..1406)
                     /gene="rplC"
                     /locus_tag="PLA01_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPD01544.1"
                     /translation="MKKFILGKKVGMTQIFNEEGLLVPVTVIEAGPMTVIQKKNPETD
                     GYSSIKVAFGDVKEKRINKPQKGVFTKLKISPKKYMKEFRIDNLEQYEVGQEIKVNEM
                     FENGDHVDVTGTSKGKGFAGAIKRHGFRRGKETHGSKYHRRVGALAACADPGKVYKGR
                     KLPGHMGVERVTIQNLDIVRVDGERNLVLVKGAVPGAKGGLLMIKETVKAVK"
     gene            complement(1490..1801)
                     /gene="rpsJ"
                     /locus_tag="PLA01_09390"
     CDS             complement(1490..1801)
                     /gene="rpsJ"
                     /locus_tag="PLA01_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPD01545.1"
                     /translation="MANKQKIRIRLKAFDHQVLDQSAEKIVDTAKRTGAQVSGPVPLP
                     TEKEIITILRAPHMYKDSREQFEMRTHKRLIDILLPTPKTVDALMRLDLPAGVDIEIK
                     L"
BASE COUNT          594 a          565 c          328 g          824 t
ORIGIN      
        1 ttgcttaatt tcggtttttg ttgccttaac atctactata aaggtgtact tcccgttagc
       61 tatttcatca ttactttttt ccgttatata cggtctgatt aagatatctt cggcagctct
      121 cattatgcgt acacctcctc aacctttgat actgcatccc tggttattat aaacttttca
      181 tgttttacta tgtcatacac attcattgtg tttacaagaa gagttttaac cccgggaata
      241 tttcttgccg acatttgaac actctcattc ttatcggcta taacaataac agcagtttca
      301 tttacattta aattcttcag aacatccacc atctgtcttg tctttatctt gtcaaagttc
      361 agactgtcca atacaagtat ttcattctca ttaacctttg atgaaagtgc tgactttaat
      421 gccaaccttc taagtttttt aggaagtgta taactgtagc ttctcggctt tggtccaagg
      481 actatgccac ctttaatcca ctgtgccgaa cgaatacttc cctgtcttgc cctacctgta
      541 cctttctgtc tccacggctt aataccacca ccgcgaactt cactttttgt ctttgtcgat
      601 tgagtaccct gtcttttatt tgccaattga ttcaccacaa cctgatgaat agcattttca
      661 ttgacatcaa ctgaaaatat attttcactt aactcaatat ctccaacgac cttgccattt
      721 atatcataaa catcaacctt cggcattcta attcctcctt tcacccatta tttaacggcc
      781 tttaccgttt ccttaatcat tagtaaaccg cctttagcac ccggtactgc tcccttcaca
      841 agaacgagat tccgttcgcc atctactctc acaatgtcaa gattctggat tgttaccctt
      901 tcaacaccca tatgccccgg cagctttctt cctttataaa cttttcccgg atcggcacat
      961 gctgccaatg ctccaactct tctatgatat ttggaaccat gggtttcctt tccacgtctg
     1021 aatccatgcc tttttatagc accggcaaag cctttacctt ttgaagtacc agttacatca
     1081 acatgatcac cgttttcgaa catttcattt acttttattt cctgtccaac ctcatactgt
     1141 tccaaattat caattctgaa ttccttcata tacttctttg gtgatatctt taactttgtg
     1201 aatactccct tttgcggctt attgattctt ttctccttaa cgtcaccaaa agctacctta
     1261 attgagctat aaccgtccgt ttcaggattc tttttctgga taacggtcat tggtcctgct
     1321 tctataacag ttacaggaac taacaaaccc tcttcattaa aaatctgcgt catacctact
     1381 tttttgccta aaataaactt tttcacctat tatccctcct atggttattg gttcacatta
     1441 cagctgctgc ttataatgcg aacttgacct ttaataccgt tgttcttcat tacagcttta
     1501 tttcgatatc tacaccggca ggcaaatcca gtctcatcaa tgcatccaca gtctttggag
     1561 tcggaagcag aatatcaata agtctcttat gtgttctcat ttcaaactgc tcacgggaat
     1621 ccttatacat atgtggtgcc ctcagtattg ttattatttc tttttctgtc ggtaacggca
     1681 caggtcctga cacctgagct ccggttctct tggccgtatc aactatcttc tcggctgatt
     1741 ggtcaagaac ctgatgatca aatgctttta gcctgattct gattttttgt ttgtttgcca
     1801 tacataatcc tccttaacat tgttacgtac aacaagaatc atttctgtac actatgccgg
     1861 gtgtttccaa tttgactata ttaaaaccca ggattcataa tttgcctata aggcatatat
     1921 acctgggctg tactattcca agttcctatg catagtttta tgcatcgcaa aatgtacagt
     1981 gacttgtcgc ccggtttcta gaatcggaca ttctccatag aagttacctg catctagtgc
     2041 agcaatctcc tatttcatcg tatgcctcgc cacaacattc aacattttac tataaatttt
     2101 tattttttgc aagtattttt ttaaaggatt tttcttcatt attgcataag aatcataaag
     2161 cagtttgaat gaatcaaatt gcaaattgca caatgcacaa taactgcccc gtccctaaat
     2221 gacctaaagc agtttgaaac gagttaaatt gcacactaat tgccccgtcc ccatataaac
     2281 aaagccctac gggattaccg tggggctttg a
//
LOCUS       DAOOJC010000392         2310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_36549_length_2310_cov_2.700665, whole genome shotgun sequence.
ACCESSION   DAOOJC010000392 DAOOJC010000000
VERSION     DAOOJC010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36549_length_2310_cov_2.700665"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..440
                     /locus_tag="PLA01_09395"
     CDS             237..440
                     /locus_tag="PLA01_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01546.1"
                     /translation="MANKNKKNNIKNERIEDSTMYGEFISSFHQWITPDRQRKVARHL
                     EEVTEELAPEDKNKDKTDRKRSN"
     gene            712..1173
                     /locus_tag="PLA01_09400"
     CDS             712..1173
                     /locus_tag="PLA01_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HPD01547.1"
                     /translation="MAKLSDLIEAFIKQMINDTDGTIEIQRNELANQFNCVPSQINYV
                     IDTRFTTERGYYVESRRGGGGHIKIRRVSIEHKGKNFLMHIINSMGDNISQQSAYVFI
                     NNFVDYEVISEREGMMLKAATSDKVLMAAPVEVRGYIRASILKNMLVSLLV"
     gene            1214..1732
                     /locus_tag="PLA01_09405"
     CDS             1214..1732
                     /locus_tag="PLA01_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HPD01548.1"
                     /translation="MFCEDLKGVGRMLCQKCQKRVAKVQLTQVINNNKSVLFLCEYCA
                     REEEKYNIGSPFSINDFFSGIMGFPYMTSVPQQTQDLVCDKCGMSYNEFNKTGKIGCS
                     NCYKVYGDRLVPLLKRLHGNIQYNGKIPSRAFSSIKVSREIIKLKEQLDATIKNEEYE
                     KAAALRDQIRNL"
     gene            1789..>2310
                     /locus_tag="PLA01_09410"
     CDS             1789..>2310
                     /locus_tag="PLA01_09410"
                     /EC_number="2.7.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HPD01549.1"
                     /translation="MKEWYIKNGPESDVVMSTRIRLARNFNDFPFPSRMNREDSEKVL
                     NKVKEAVFGRSSAIGENFQFEDIQKMDPIQRQALVEKHLISPDLAESQSNGGVIISDD
                     EKISIMINEEDHLRIQCLFAGLQLKEAWDLCNKIDNLLEETIDYAFDERFGYLTCCPT
                     NIGTGIRASAMLHL"
BASE COUNT          807 a          338 c          534 g          631 t
ORIGIN      
        1 ttgttttagg aatagccctt gccaaaattg tatataggga aaggaaaagc attttcaagt
       61 tttcaataaa ggacaaccca ttgaattttt taggaagaca ctccctttgg gtatatttat
      121 ttcaccagcc tgttattttg ttattcttga tattaatact gggacaaacc tcatgaatac
      181 atctttactc ctttgataaa aataagttat attttatttt aaaggagtta tgaagaatgg
      241 caaataagaa taaaaagaac aatatcaaaa acgagagaat cgaagattca acaatgtatg
      301 gtgagtttat ttcgtctttc catcaatgga taacgccaga cagacagagg aaagttgcac
      361 gtcatttgga agaagtgaca gaagagcttg ccccagagga caaaaacaaa gataaaaccg
      421 acaggaaaag aagtaattag gcaaaaaaag agcaattact gtgccggata acaggtatta
      481 aaataaatgc ttgcctattg cctgctatcg gttaaattaa tcttgattat ggaaaagaaa
      541 taaattataa tattattgaa ggtcaaagat agtcaaagtg aacttgctgg gttgatgctg
      601 aacattgtgt tatgggtgat gtattgcatc cccattaaag taaaaagagg gccctgcctt
      661 ataaaaggca gggttacggg attaaaatca acacaaaaga aggtggcgga tatggctaag
      721 ctcagtgacc ttatagaagc atttataaag caaatgataa atgacactga cggtactatt
      781 gaaattcaaa gaaacgaact tgccaatcaa tttaattgtg tgccttcaca gataaactat
      841 gtaattgaca caagattcac aacagaacgg ggatattacg tagagagccg aagaggcgga
      901 ggcggacata ttaaaataag gcgcgtaagt attgagcaca agggtaagaa ttttcttatg
      961 cacattatca attcgatggg agataacatc tcacaacagt ctgcatatgt gtttatcaat
     1021 aattttgttg actatgaggt aatatccgag agggagggaa tgatgcttaa ggcagcaaca
     1081 agcgataagg tactgatggc agcacctgtt gaagtcagag gatatataag ggccagtata
     1141 ttgaaaaata tgttggttag cttattggtt tgaacgattg ttgtaaaaat cctgttgata
     1201 ccgtcaggag tatatgtttt gtgaggattt gaaaggagtg gggcggatgc tgtgccagaa
     1261 atgtcagaaa agggttgcga aagttcagct tacgcaagtc ataaacaata ataaaagtgt
     1321 attatttctt tgcgaatact gtgcccggga ggaagaaaaa tataacatcg gttctccttt
     1381 tagtataaac gattttttct caggaataat gggatttccg tacatgactt cggttccgca
     1441 gcaaactcag gatctggtat gtgataaatg cgggatgagc tataatgagt ttaacaagac
     1501 cgggaagata ggatgctcca attgttacaa ggtatatgga gacaggcttg taccgttgct
     1561 taaaaggctc catggcaata ttcaatacaa tgggaagata ccttccagag cttttagcag
     1621 tataaaagtg tcaagggaga ttataaaatt gaaggagcag ttggatgcta ccataaaaaa
     1681 tgaagagtat gaaaaagcag cagcattaag ggatcagata aggaatctgt agtctaatat
     1741 cggcaaatag atttggcaaa gtttttttgg atctgggagg tggattttat gaaggagtgg
     1801 tatataaaaa acggtcccga gtcagatgtg gttatgagta caaggattcg gcttgcccga
     1861 aattttaatg attttccctt tccatcccga atgaataggg aagatagtga aaaggtatta
     1921 aataaagtaa aagaagctgt tttcggaaga agcagtgcaa ttggtgaaaa cttccaattt
     1981 gaggatattc aaaaaatgga ccctatacag aggcaggctt tggttgaaaa acatctcata
     2041 agtcccgatt tggcagaaag tcagtccaat ggcggagtga taataagtga tgatgaaaag
     2101 atcagtataa tgattaacga ggaggaccat cttagaatac aatgtctgtt tgcgggtctg
     2161 cagcttaaag aagcctggga tttgtgcaac aagatagaca accttttaga ggagaccata
     2221 gattatgctt tcgatgaaag attcgggtac ctaacctgct gtcccaccaa tattgggaca
     2281 ggaataaggg catcggcaat gcttcacctt
//
LOCUS       DAOOJC010000393         2305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_36664_length_2305_cov_2.443111, whole genome shotgun sequence.
ACCESSION   DAOOJC010000393 DAOOJC010000000
VERSION     DAOOJC010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36664_length_2305_cov_2.443111"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLA01_09415"
     CDS             <1..289
                     /locus_tag="PLA01_09415"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPD01550.1"
                     /translation="VNIENASYGATNCAERTAIFKAVSEGERKIEAVAITSDSDNLTF
                     PCGICRQVIAEFGDENTRIICANKSGEFKVFKLAELLPNAFVFSSENALIK"
     gene            373..1266
                     /gene="era"
                     /locus_tag="PLA01_09420"
     CDS             373..1266
                     /gene="era"
                     /locus_tag="PLA01_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPD01551.1"
                     /translation="MAFKSGFISIVGRPNVGKSTLLNRFTGEKIAIMSDKPQTTRNTI
                     KAIDTGEDYQMIFIDTPGIHKPKTKLGDYMVNVAMDTLNEVDLVLFLVEATDKKPGAG
                     DSYIAEQLKQINTPVFCLINKIDLVEKEQILPVISAYKELMEFSQIIPVSALEYESAE
                     LVKKEIKKVLPEGPKYFPEDMITDQPEKIIAAEIIREKILELVSDEVPHGVGVEVISF
                     KNREDKDILNIQANIYCEKKTHKGILIGKEGRMLKKIGSLARVEIERLFGTKVFLELW
                     VKVKNDWRNNDLMLKTLGYNK"
     gene            1382..1528
                     /locus_tag="PLA01_09425"
     CDS             1382..1528
                     /locus_tag="PLA01_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HPD01552.1"
                     /translation="MLKEFAWKMFEKTGDISMYMFLKEIEEKTRTVDETEIAMDEVAI
                     STHH"
     gene            1648..>2305
                     /gene="recO"
                     /locus_tag="PLA01_09430"
     CDS             1648..>2305
                     /gene="recO"
                     /locus_tag="PLA01_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPD01553.1"
                     /translation="MSYLKTTGIVIKEVNTGEADKIVTIFSKTKGKISGSARGARRPK
                     SRLVAGTQLLCYSEFVLFKGKDLYSINSCDVIEPFYDIRNDIERLTYAAHMTELINDV
                     IQENQPATKVLQLFLNSLYLLSKTSRSPVQIVRVFELRLLSILGYAPSVCGCVVCGSS
                     QFDSMSFSFQKCGFICKSCTMQDKSAAELSEGAAKALNYIVYSPLNSLFSFEVSDSVL
                     N"
BASE COUNT          758 a          345 c          537 g          665 t
ORIGIN      
        1 ggtaaatatt gagaatgcaa gctatggtgc caccaactgt gccgaaagga cggctatttt
       61 caaggctgtg tcggagggtg aacgcaaaat tgaggctgtg gctataacca gtgacagcga
      121 taatctgact tttccctgtg gcatttgccg gcaggtgatt gccgagtttg gggatgaaaa
      181 cacaaggatt atttgtgcaa ataaaagcgg tgaattcaaa gtttttaaac ttgcggagct
      241 tttgcccaat gcttttgttt tttcctcaga aaatgcatta attaagtgag ttaaaattag
      301 aataaaggat attatataga ttagaaatac actgtgtaaa aagcatgtga agatttaacc
      361 ggaggtaagt atatggcatt taaatcaggt ttcatttcaa tagtgggaag gccgaatgtg
      421 ggaaagtcta cactattaaa caggtttaca ggcgagaaga tagcaattat gtcggataaa
      481 cctcagacta caagaaatac tataaaggca attgatacgg gtgaggatta tcaaatgatt
      541 tttatcgata ctcccggaat acataagcca aagacaaaat tgggggacta tatggttaat
      601 gttgccatgg atacattaaa cgaggttgat ctggtattgt ttctggttga agctacggac
      661 aaaaagcccg gggctggtga ttcatacatt gctgaacaac tgaagcaaat taatacccct
      721 gtattttgct tgataaataa aatcgatctt gtggaaaagg agcagattct gcctgtcata
      781 tccgcttata aggagcttat ggaattctct cagattatac ctgtttccgc tcttgaatat
      841 gaaagtgcag agttggtaaa aaaggagata aaaaaggtct tgcccgaggg accgaaatat
      901 tttcctgagg atatgattac ggaccaacct gaaaaaataa tagctgctga gataattagg
      961 gagaagatat tggagcttgt gagcgatgaa gtacctcacg gtgtaggagt tgaggttatt
     1021 tcgtttaaaa acagggagga taaggacata ctgaatattc aggcaaatat atactgtgaa
     1081 aagaaaaccc acaaaggcat tctgattggc aaagaaggca gaatgcttaa gaaaatcgga
     1141 agtctggcaa gagttgagat cgaaaggctt tttggtacaa aggtttttct ggagttatgg
     1201 gtaaaggtga agaatgactg gcggaacaat gatctgatgc ttaaaactct ggggtataat
     1261 aaatagggca taaaaggttt gtatatgata aattttcaag tatacaaata tttttttcgt
     1321 gtaacctaat tattattaag ataagtgtgg tttatcaaat aataataagg ggaaggcatt
     1381 tatgttaaaa gagtttgctt ggaaaatgtt cgaaaaaaca ggtgatatct caatgtatat
     1441 gtttttaaag gaaatagagg aaaaaaccag aactgttgat gagactgaga ttgccatgga
     1501 tgaggttgcc ataagcactc atcattaata cccggtaaca taattaattt ccgggacact
     1561 ttgagcacaa gcacaaggaa tgcattctaa atattttgtt tagatggaaa accggttttt
     1621 taaaatatta ggggaatcat atcggatatg agctatttaa aaacaacagg aattgttata
     1681 aaagaagtaa ataccggaga ggctgacaag atagtaacga tattttccaa gaccaaaggg
     1741 aaaatatccg gttcagcccg gggggcaaga aggcccaaaa gccgcctggt tgcaggtact
     1801 cagcttttgt gctacagtga atttgtgctt tttaagggga aggaccttta ctcaattaat
     1861 agttgtgatg ttatcgaacc cttttatgat ataagaaatg acattgaaag actgacctat
     1921 gccgcacaca tgacggaact gataaacgat gttattcagg aaaatcagcc tgcaaccaag
     1981 gttttgcagc tgtttttaaa ctctctttat ctcctttcca aaaccagcag atctcccgtg
     2041 caaatagtca gagtatttga attacgtctc ctttcaattt tgggatatgc accttcggtg
     2101 tgcggttgtg tagtttgcgg aagcagtcag tttgacagca tgtctttcag ctttcagaag
     2161 tgcggtttta tttgcaaaag ctgtacaatg caggataaaa gtgctgcaga gctttctgaa
     2221 ggtgctgcga aggcattgaa ttatattgtt tacagcccat taaatagcct gttcagtttt
     2281 gaggtttcgg acagtgtttt aaatg
//
LOCUS       DAOOJC010000394         2300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_36779_length_2300_cov_3.880178, whole genome shotgun sequence.
ACCESSION   DAOOJC010000394 DAOOJC010000000
VERSION     DAOOJC010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36779_length_2300_cov_3.880178"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PLA01_09435"
     CDS             complement(<1..276)
                     /locus_tag="PLA01_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPD01554.1"
                     /translation="MPKQKSFFVCQECGYESRGWLGKCPACNQWNTFVEEIVETGHKN
                     SRGMAPINVKLVSINDIDIEGEERYLTGMKEMDRVLGGGIVRGSLVLV"
     gene            complement(328..2262)
                     /locus_tag="PLA01_09440"
     CDS             complement(328..2262)
                     /locus_tag="PLA01_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HPD01555.1"
                     /translation="MTKTYFNDAIIGNSKMLACLTASGELVRLFWPHIDYPQHIEKMA
                     SGIFYIGQKNSTSWFNEHDWKHTQYYVEDTNILKTLCENESRGLRVEQTDYVLKDRDI
                     LVRQYIIENTGSTEVELGFVNYSSMVTANPDLRSTLFDFNVDSLIHYKHNYYISVSSD
                     REVMQFQLGNNAYNCASYTELCGFDSIGMMNDGAISFNIGKVGPGCKVPFNLFICASH
                     TLKEVKQMVKLCRQLSLKEEYENTLIYWRDMLKNARQIKTGDDSIDALYNRSLLVFKL
                     MSDEQTGGLLASAEIDEGFTMCGRYAYCWGRDAAFITGALDAVGFTDAVDRFYKWAVM
                     TQDEDGSWQQRFHMDGNLAPSWGLQVDETGTLIWGMYRHYEVTKNIEFLKEMWESIKL
                     GIDFLTGFIDSDTGLPKPSYDLWEERVGEHTYSSAAVYGGIMAGVEVAKILNAPSEII
                     DKWNEAAQSIKSAIEKNLWKDEAKRFIRSVRTKLNPWGSEHSAHTTIVKVNEKGYYRD
                     VTLEDWTVDISLLGVSIPFGVFDVKDIRVKETVEAIERALTSHPVGGIKRYENDNYVG
                     GNPWVLATLWVALYYIEVEEYDKAKEYFMWAVKSCTALGLLPEQVSKDSGRPCWVIPL
                     TWSHAMFVLVLAGLKDAGAI"
BASE COUNT          610 a          615 c          386 g          689 t
ORIGIN      
        1 tacaagaaca agggaacccc ttacaattcc tccccccagc acgcggtcca tttccttcat
       61 gccggtaaga taacgctctt caccctctat gtcaatatcg tttatgctga ccagttttac
      121 atttatcgga gccatgcccc tgctgttttt atgtccggtt tcaacaatct cttcaacaaa
      181 ggtattccat tgattgcacg ccggacactt tcccagccag ccccggctct catagccaca
      241 ttcctggcac acgaaaaatg atttttgttt cggcatcttt tctcctaacc agggtttatt
      301 acatcaaaaa ccccaaatga ttttatccta tatagcacct gcatctttaa gccctgccag
      361 aaccaataca aacatggcat gggaccatgt cagaggaatt acccagcaag gcctgccgct
      421 gtccttgctc acctgctccg gcaaaaggcc aagagcagta catgatttca ctgcccacat
      481 aaaatattct tttgccttat catactcttc aacttcaata tagtataggg ctacccagag
      541 tgttgccaga acccaggggt ttcctccaac gtagttgtca ttctcatacc tttttattcc
      601 tcccacagga tgagatgtaa gggctctttc aatggcctcc actgtttcct taacccttat
      661 gtccttcaca tcaaaaacac caaagggaat agacacccca agaaggctta tatctacggt
      721 ccaatcctcc aatgtaacat ctctataata acccttttca ttgaccttta caatagttgt
      781 atgtgcagag tgctcgcttc cccaaggatt aagcttggtc ctcacgctcc ttatgaaccg
      841 ttttgcctcg tccttccaaa ggtttttttc tatggcagac ttaatgcttt gtgccgcctc
      901 attccactta tctattatct cggagggtgc atttaaaatc ttagccacct caacccctgc
      961 cataatacca ccatataccg cagcactgga gtaggtgtgt tctcccaccc tttcctccca
     1021 aaggtcataa ctgggcttgg gcaatccggt atcggaatct ataaatcccg ttaaaaaatc
     1081 aatgcccaac tttatgcttt cccacatttc tttaagaaat tcaatgtttt ttgtaacctc
     1141 atagtgcctg tacattcccc atataagtgt tcccgtctca tccacctgca atccccatga
     1201 cggtgcaagg tttccgtcca tatggaacct ctgctgccat gacccatcct catcctgtgt
     1261 cataacagcc catttataaa acctgtctac cgcatcggta aatccgactg catccaatgc
     1321 accggtaata aatgcggcat cccttcccca gcaataggca tagcggccac acatcgtaaa
     1381 gccttcatca atctcggcag aggctaaaag ccctcccgtc tgttcgtcgg acatcagctt
     1441 gaataccaaa agagacctgt tatacagagc atcaatacta tcatcccctg ttttaatctg
     1501 tctggcattc tttaacatat ccctccagta tataagggta ttttcatatt cctctttaag
     1561 gcttagctgt ctgcacagct tcaccatctg tttgacctct tttaatgtat gggatgcaca
     1621 tataaaaagg ttgaagggca ctttgcagcc gggtccgact tttcctatgt taaaggaaat
     1681 tgcaccgtca ttcatcattc caatactgtc aaaaccgcaa agctccgtat atgacgcaca
     1741 gttataagcg ttattgccca attgaaactg cattacttcc cggtccgatg aaacagaaat
     1801 atagtaattg tgtttataat gaattaacga atcaacattg aaatcaaaaa gagtacttcg
     1861 caaatcagga ttggcggtaa ccattgaaga atagttcaca aagcccagtt ctacttccgt
     1921 gctgcctgtg ttttcaatta tgtattgtct caccagtatg tccctgtctt tcaatacata
     1981 atccgtctgc tcaactctaa gccctcggct ttcattttca cacagggttt ttaaaatatt
     2041 ggtatcctcc acataatact gggtatgctt ccaatcatgc tcattaaacc acgaagtgct
     2101 gtttttttga ccgatataaa atattcctga tgccattttt tcgatgtgct gcgggtaatc
     2161 gatatgaggc cagaaaagcc ttacaagctc gccgctggcc gtcaggcatg caagcatttt
     2221 ggaatttccg attattgcgt cgttaaaata tgtttttgtc ataaaaatcc ccccgtaacc
     2281 tttaattgtt aaattcctgg
//
LOCUS       DAOOJC010000395         2287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_37056_length_2287_cov_3.465502, whole genome shotgun sequence.
ACCESSION   DAOOJC010000395 DAOOJC010000000
VERSION     DAOOJC010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37056_length_2287_cov_3.465502"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLA01_09445"
     CDS             <1..452
                     /locus_tag="PLA01_09445"
                     /inference="COORDINATES: protein motif:HMM:NF027524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPD01556.1"
                     /translation="AFIRRGYDLSCFSEAVAKLKERNIRVVVHMIIGLPGESREDILD
                     TVRLVAKLGVWGVKFHMLYIQKDTDLYEYYCSNPFSVLTLDEYVNIIADALEILPPEI
                     VVHRVTGDGVKRLLEAPLWTLDKLRVLSEIDRVLRERNSYQGCRQKV"
     gene            519..1739
                     /locus_tag="PLA01_09450"
     CDS             519..1739
                     /locus_tag="PLA01_09450"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD01557.1"
                     /translation="MIYRAEPGTLMVFNADDGITAAVSGKELYSLKKYLETEAQNEFI
                     NRLIDLKLMPEEISECERQELLKEIEKTENISAPVRSFAVPESIHIDITTFCPLKCPQ
                     CYKDLSQDIEMPLGKFFDIIERAKALKVFQIALGGGEPLTVKDLPVFVKKVTSCKMSC
                     TITTSGYGLTAELLDDLKESGVNHIQVSLNGSTREINSYSRDGFSEAVSALNILSKSS
                     VQFGINWVARRDNVKDFENIVTYAKELKADNINILRYKPSVREDYGKNVLTGDDFNLL
                     VAAIRKVRGIKIKIDSAFSNILCQIYANRINPVNCGCGAGRRFMMIDPVGRLKPCSHL
                     SMVSQEQDILQYWLNSPDIAKLRQIEESIYGDCKNCSYLKACRGCRAICERLHNDINS
                     GEINCPVFNNKEMV"
     gene            1740..>2287
                     /locus_tag="PLA01_09455"
     CDS             1740..>2287
                     /locus_tag="PLA01_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01558.1"
                     /translation="MFTYWKGYEIDGSSTAYNIVVPQREMISEVLRYFISLKIIDRKS
                     DISLSYTEREKSASNKYECENGKYKHDETEKICEILESLSVDSFFINADCIHPDKLFH
                     QVRNELYSLSKTRPGAKDRVYNYINPTVSPFRFGPDLGVTFEVSLEEEQAIENHEPEG
                     LVFNRDALVNNMRGQHFTITLG"
BASE COUNT          715 a          366 c          542 g          664 t
ORIGIN      
        1 ccgcatttat aagaagaggt tatgaccttt cgtgcttttc cgaagcggtc gcaaagctaa
       61 aggaaagaaa tatccgtgtg gtggtacata tgattatagg tttgccgggc gaaagcaggg
      121 aggatattct tgacacagtt agattagtcg caaagcttgg tgtatggggc gttaaatttc
      181 atatgctgta tatccaaaag gacactgacc tttatgagta ttattgttcc aatcctttta
      241 gtgtgcttac tttggatgaa tacgtaaata ttattgctga tgctctggaa atcctgcctc
      301 ctgaaatagt ggtacatcgg gttacagggg atggagtaaa aaggctttta gaagcaccct
      361 tgtggacatt ggacaaactc agggtgttgt cggaaattga ccgggtatta agggagagaa
      421 acagctatca gggctgccgg caaaaggttt agagcaacat ataaatattt tctgtgaact
      481 tgatttttat taaatttata tgaaggggaa gaaatactat gatttaccga gctgaacccg
      541 gcacgctgat ggtgtttaat gctgatgacg gtattactgc tgctgtttcc ggcaaggaac
      601 tgtatagctt gaaaaaatac ttggaaaccg aggctcaaaa tgaatttata aatagattaa
      661 ttgatcttaa attaatgccg gaggaaatat cggaatgtga aaggcaggag cttttaaagg
      721 aaattgaaaa gacagaaaat attagtgctc ccgtaaggtc ctttgctgtt cctgagtcaa
      781 tacatattga tataactacg ttttgtccgc tcaaatgtcc gcaatgctat aaagatttat
      841 cacaggatat agaaatgcct ttgggaaaat tctttgatat aatagaacgg gcaaaagctt
      901 tgaaggtatt ccaaatagct ttgggcggtg gtgaaccttt gacggttaag gatttgcctg
      961 tattcgtaaa aaaggttaca tcatgcaaaa tgtcatgtac aataactaca agcggttatg
     1021 gactgacggc agaacttctt gatgatttaa aagaatccgg tgtcaatcat attcaggtat
     1081 cgctgaacgg gtctacgagg gaaataaatt catactcccg cgatggtttt tccgaagcag
     1141 tttcggccct taatatactg tcaaaaagca gtgtgcaatt cggtataaac tgggttgcaa
     1201 gaagggataa tgtgaaggac tttgaaaaca tagttactta tgcaaaagaa ctgaaggctg
     1261 ataatataaa tatattgagg tataagccgt cggtcaggga ggattatgga aagaatgtat
     1321 tgaccgggga tgatttcaac cttcttgtgg cggcaattag aaaggttagg ggcataaaaa
     1381 ttaaaattga ttctgcgttt tccaatattc tgtgtcaaat atacgcaaat cggataaacc
     1441 ccgtcaactg tggatgcggt gccggacgta ggttcatgat gatagatcct gtgggcagac
     1501 ttaaaccttg cagtcatttg agtatggttt cacaggagca agatatactg caatattggc
     1561 tgaattcacc ggacattgca aagctaagac aaattgagga atccatttac ggggactgta
     1621 aaaattgtag ttatttaaag gcttgccgcg ggtgcagggc aatttgcgaa agattacata
     1681 atgacataaa ttcgggagag attaactgcc cggtattcaa taataaggag atggtttgat
     1741 tgtttactta ttggaaagga tacgagattg acggttcttc taccgcctat aacattgtag
     1801 ttccacaacg ggagatgata tctgaggttt tgaggtattt tatcagcttg aaaattattg
     1861 accgcaaatc ggatatttcc ctttcatata ccgaaaggga aaagagtgct tcaaacaagt
     1921 atgaatgtga gaatggaaaa tataaacatg atgaaacaga gaaaatctgt gaaattcttg
     1981 aatcactttc tgttgattcc ttttttatta acgcagattg tattcatccg gataagcttt
     2041 ttcaccaggt gcgtaacgaa ctgtatagtt tatctaaaac acggcccggt gccaaggaca
     2101 gggtatacaa ttatataaat cccacggtta gtcctttccg ctttggcccg gacttggggg
     2161 taactttcga agtttctctt gaagaggagc aggcaataga aaaccatgaa ccggaaggat
     2221 tggtctttaa cagagatgcc ctggttaata atatgagagg acagcatttt accattaccc
     2281 ttgggtg
//
LOCUS       DAOOJC010000396         2275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_37322_length_2275_cov_2.876126, whole genome shotgun sequence.
ACCESSION   DAOOJC010000396 DAOOJC010000000
VERSION     DAOOJC010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37322_length_2275_cov_2.876126"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLA01_09460"
     CDS             <1..213
                     /locus_tag="PLA01_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HPD01559.1"
                     /translation="ENSINIQTVKEYAENKLGMHMPDSYQITYVKVPQKDITIVSETA
                     QLEESKNSGIFSAFLNKMNLIVSMLY"
     gene            408..>2275
                     /locus_tag="PLA01_09465"
     CDS             408..>2275
                     /locus_tag="PLA01_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPD01560.1"
                     /translation="MAANTTLIMKKRLLVVLTVFSLLVFALIARVGWLQLVKGEELQK
                     MAFEQQNSDREIPPRRGSILDRKGEALAISATVDRIVVNPTEIDESDEEEISQKLAEF
                     LDMDKDAILKKVSNNKSRYEIIKKRVEKNVGDRIRQWKSEENIHGVYIDEDSKRYYPS
                     GNLAAHIIGFTGEDNQGLDGIEKTMEKYLKGVPGKILSEVDARGRQMPNYQNKRIDPQ
                     DGLNVVLTIDETIQHHATEALQKALDDNKVANGGIAIVLDPRNGEILALVSKPDYNLN
                     SPRACPPGADPATWTGTSQEDIDFLYKTVWRNKAVSDTYEPGSTFKAITSAIAFEEGG
                     VTPETRTSDFPIELQGWTINCWSTVLHGEESFREAVYNSCNPAFVRAALSVGVEKFYK
                     YVRAFGFYDKTGIELPGEAKGNFHAKPQEIDLATASFGQTFTITPIELITAYGALANG
                     GTLIKPRLVKEVVDSEGNVVVKTQPEVVRNVISKETSQTLLNVLEGVVSEGTGRNAYV
                     KGYRVAGKTGISETTVKGVYIASFAAIAPADNPRICVLVALDDPRGESHFGGTIAAPV
                     AGKIVEETLKHLGVERRYSEKDLKMIAEEVYVPDIRDKSISDAKKLLNQMGLKCRI"
BASE COUNT          712 a          384 c          588 g          591 t
ORIGIN      
        1 gaaaacagta tcaatattca aacggttaag gaatatgcgg aaaataagct tggcatgcat
       61 atgcccgaca gttatcagat tacatatgtg aaggttccgc aaaaggatat tacaattgta
      121 tcggaaacgg ctcagcttga ggaaagcaag aattccggca ttttctcagc ctttttaaac
      181 aagatgaatt taatagtaag tatgctttat tagaaaaaac attttatatt tataagtcga
      241 aattaaaaga aaaccgctta ggggggaaga taggggggca gattatcaat tagcctcttt
      301 ttcttattat taatattgta tttaatagta ttgtatcgca ttaactgtag tataatgata
      361 gcatacgcat gtttttttgt tgtttgtata gatggagggg gataggtttg gctgctaata
      421 ctacactgat catgaaaaag agactgttgg tcgttttaac ggttttttct cttctggttt
      481 tcgctttaat tgccagggtt ggatggcttc agttggtcaa aggcgaggaa cttcaaaaaa
      541 tggcctttga acagcaaaac agtgacaggg aaatacctcc aagaagaggg tctattctgg
      601 acagaaaagg agaagcgttg gctataagtg ccactgttga ccggatagtt gtaaatccca
      661 cggagattga tgagtccgat gaggaagaga tttcgcaaaa gttggcggaa ttcttggata
      721 tggataagga cgcgattctg aaaaaagtaa gcaataacaa aagccgttat gagataataa
      781 aaaaaagagt ggaaaagaat gtcggtgacc ggataagaca gtggaagagc gaagaaaata
      841 tacatggcgt atatattgat gaggattcaa aaaggtatta tcccagcgga aaccttgccg
      901 cacacataat cggctttacc ggtgaagaca atcaaggact tgacggaata gaaaaaacaa
      961 tggagaagta tttaaaaggt gtaccgggca agatattgag cgaggttgat gcccgcggaa
     1021 ggcagatgcc caattatcaa aataagcgta tagatcccca ggatgggctg aatgtggtgc
     1081 ttaccattga tgaaaccatt cagcatcatg caacggaggc actgcaaaag gccctcgatg
     1141 acaacaaggt tgcaaacggt ggtattgcca ttgtgctcga ccccagaaac ggagaaattc
     1201 ttgccttggt gtcaaagccg gactataatt taaacagccc gagggcatgt ccgccgggag
     1261 ctgatccggc aacctggacc ggaaccagtc aggaggatat agattttcta tataaaactg
     1321 tatggagaaa caaggcggtt tccgatacct atgaaccggg ttctaccttc aaagccatta
     1381 cttcggcgat tgcgtttgaa gagggtggtg tcacccccga aaccagaaca agtgattttc
     1441 ctattgagct tcagggctgg acaattaatt gctggtctac agttcttcat ggtgaagagt
     1501 cttttagaga agcggtttat aattcctgta accctgcatt tgtcagggca gctctgagtg
     1561 ttggtgtgga aaagttttat aaatacgtta gggcttttgg attttatgac aagacaggaa
     1621 tagagcttcc cggcgaggct aagggaaact ttcacgcaaa gccccaggag attgaccttg
     1681 caactgcttc cttcgggcag acgtttacaa taacccctat tgagcttatt accgcttatg
     1741 gtgctctggc aaacggcggc acgcttataa aacccaggct ggttaaagag gttgtcgatt
     1801 cggaaggaaa tgttgtagtg aaaactcagc ctgaggttgt aaggaatgtt atttctaaag
     1861 aaacatcaca aactttgttg aacgttctgg aaggggttgt atccgaaggt accggtagaa
     1921 atgcgtatgt caaaggatac agggtggcag gtaaaacagg tatatcggaa acaacggtaa
     1981 aaggtgtgta tatagcttcc tttgcagcga ttgcaccggc ggacaatccg cgtatatgtg
     2041 tgctggtagc actggatgac ccgaggggtg aatcacattt cggtggtact attgctgcac
     2101 cggttgccgg taagatagtc gaggagacgc taaagcatct tggtgtggag agaagatact
     2161 ctgagaagga tttaaagatg attgcagaag aggtgtatgt accggatata agggataaga
     2221 gcataagtga tgccaaaaaa cttttaaacc aaatgggatt gaagtgtagg ataga
//
LOCUS       DAOOJC010000397         2274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_37345_length_2274_cov_2.970257, whole genome shotgun sequence.
ACCESSION   DAOOJC010000397 DAOOJC010000000
VERSION     DAOOJC010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37345_length_2274_cov_2.970257"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    80..359
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            472..714
                     /locus_tag="PLA01_09470"
     CDS             472..714
                     /locus_tag="PLA01_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01561.1"
                     /translation="MIFLKILMFLSIAAGFATVLVAKVIVKRFKINEKTKCDFEHEMT
                     DEELEKYLFNKAVLNIKMLGMLIALPGLILVLFIYR"
     gene            758..2274
                     /locus_tag="PLA01_09475"
                     /pseudo
     CDS             758..2274
                     /locus_tag="PLA01_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616301.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
BASE COUNT          730 a          365 c          562 g          617 t
ORIGIN      
        1 tgaatagcta aaaagtttct gtcaaaatgc cttgacatgt gaagaggatt aaattattat
       61 gtttttaaga ctaattaaag aagaaatgat gatcaggagg agtaagcggt aacgacttat
      121 tagagagggg aaatcggagg ctgaaagttt cccttaagtg acagactgca gaaggtagcc
      181 tgggagttcc ggagttgaaa ggacacgggt tttaatgaag aacggtctta gtaggctttg
      241 gcggtacaga gccgttattt cgatgcttta taaggatatt aaatccttat tgaggttgag
      301 atgcctattt aataatagga attttggtgg taacgcggaa aaagtctttc gtccaattgt
      361 aggatgaaag gcttttttat atagcagatt caaatgctcg tatcaattcg gcggttccga
      421 ttgggaagta agttgtaggc gagggaaata cctataagga gagaagggta aatgattttt
      481 ttgaaaatac ttatgttttt atctatcgca gccggttttg caacggtttt ggttgcaaag
      541 gttattgtga aaaggtttaa aataaatgaa aaaactaaat gtgattttga acatgaaatg
      601 acagatgaag aactcgagaa atatttattt aataaagcag tactcaatat aaaaatgctg
      661 ggaatgctta ttgctcttcc cggattgata ttggtgctat ttatttatag atagtccgaa
      721 taattaattc ttatataaat aattggaggt ttttagtatg agtttagaaa aaaatattgc
      781 caagacctat gacccaaaac aggttgaaga cagattgtac ggcgagtgga tggaaaaggg
      841 ttattttcat gccgaaatag acaaagataa aacacctttt accattgtta ttccaccacc
      901 gaatattaca ggacagctcc atatgggaca tgccctggat gaaacccttc aggacattct
      961 tataagatgg aagaggatgc aggggtattg tgccctgtgg ttacccggta ccgaccacgc
     1021 cagtatagcc acagaagcca agatagttga agccatggcc aaggaaggca taaaaaaaga
     1081 ggacatagga agagaaaagt ttttggagag ggcatgggat tggaaaaagc attatggtgg
     1141 aaggattgtt gaacagctta aaaagcttgg aagttcctgt gactgggacc gtgaaaggtt
     1201 taccatggac gaagggcttt caatggctgt tatagaggtt tttattaggc tttataaaaa
     1261 aggccttata tacaggggcg aaaggatcat aaactggtgc ccgaaatgct gcacctctat
     1321 atccgatgcc gaggtggaat acgaagaaag agccggccat ttttggcaca taaaataccc
     1381 cataaaggac agcactgagt ttgttgttgt tgcaacgaca agacctgaaa ccatgctggg
     1441 ggatactgca gttgccgtac atcctgacga tgaaaggtat aagcatcttg tggggaaaac
     1501 cgttattctt cctctaatga ataaggagat accggtcatt gccgatgaat atgttgaaaa
     1561 ggatttcggt acgggtgttg ttaagataac tcccgcccat gacccgaatg actttgaagt
     1621 gggcttaagg cataatcttc cgcaggtaag ggttatgaac gatgatgcaa caatgaatga
     1681 attggccgga aagtatcagg ggttggacag gtatgaagca agaaaacaga ttgtaaagga
     1741 tctggaagaa tttggcctgc tatttaagat tgaagaccat acccataatg tcggtacatg
     1801 ctacaggtgt tccacagtaa ttgagccaat aatctcaaaa cagtggtttg ttaagatgaa
     1861 gcctcttgca gagccggcca ttgaagtggt taaaaacggt acaataaaat tcgtgcctga
     1921 aagattttca aaaatatatt tcaactggat ggaaaacatt caggattggt gtatctaaag
     1981 acaactgtgg tggggtcaca gaattccggc atattactgt caggaatgcg gatacacaat
     2041 ggtagacagt gatatgcccg atgtttgccc gcagtgcggc agctcaagaa ttgaacagga
     2101 ccaagatacc cttgacacat ggttcagctc tgcactatgg ccattctcaa ccctcggatg
     2161 gccggataaa actgaggacc ttaaatattt ctacccgacc gatgtgcttg taaccgggta
     2221 tgatataata ttcttctggg ttgcaagaat gatattctcc ggcctggagc atat
//
LOCUS       DAOOJC010000398         2268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_37477_length_2268_cov_4.424763, whole genome shotgun sequence.
ACCESSION   DAOOJC010000398 DAOOJC010000000
VERSION     DAOOJC010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37477_length_2268_cov_4.424763"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLA01_09480"
     CDS             <1..369
                     /locus_tag="PLA01_09480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPD01562.1"
                     /translation="IENGFKAYLYKIARNLALRYLKKRQHYCFFSLEGLEDDPESEML
                     VEMVVQSEERSRILRKCMEHLNYDYCEVLYLHYFENMSYAQAAQVMGKSIKQIDHLLE
                     RGKKRLRSLLEREGITDAKY"
     gene            356..709
                     /locus_tag="PLA01_09485"
     CDS             356..709
                     /locus_tag="PLA01_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HPD01563.1"
                     /translation="MRNTDERLAAVKRRVEELEQKKRQLRYRYIAMSAVAACLVIIIG
                     MGFAMPGIIAGLYGRDYSNTGMMASIFYEGKVLGYVLIGILSFVLGVCLTVLCFLLRP
                     EKQRDKEDHNNDRTH"
     gene            693..1322
                     /locus_tag="PLA01_09490"
     CDS             693..1322
                     /locus_tag="PLA01_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01564.1"
                     /translation="MIELIDNSVQLAVLTVCCIYSAIRSIRRNDHVWFLLTCFYGAYA
                     LGLVYWLLFIVFHSKTPSISPVSDLSWMASVLFILVMQNTLRLPGESTYRPILAWTAP
                     VFSTVMCFFFFHWGDYFLNVLWAALMGACGYQALRGLLWARRQSNGMNSSLYFHITVL
                     TFLLVEYSLWLASCFWKTESLLNPYYWFDFLMSATFFTFLPSVKKVVTE"
     gene            1319..1954
                     /locus_tag="PLA01_09495"
     CDS             1319..1954
                     /locus_tag="PLA01_09495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HPD01565.1"
                     /translation="MIYIENVFICITAPLLIAMLCGDRNNRPVFIFLISGIGMCLLSA
                     YINTFVASCYGVDMIQATVELAPAIEETMKLLPVLFYLLVLEPEQREAQSAIFYVAAG
                     FATFENVCYLVENGTSRILFLLMRGFGAGAMHIVCGAIVGYGLLYVWSRSWLKVAGTF
                     GLLCIAITFHAIYNLLLSVGGTAQTVGLLIPVLTVLSGIGITKILRFKSSK"
BASE COUNT          578 a          430 c          537 g          723 t
ORIGIN      
        1 atcgaaaacg gattcaaggc ttatttgtat aagatcgcca gaaaccttgc attacggtat
       61 ttgaaaaaac gtcagcatta ttgttttttt agtcttgaag gcttagagga tgatccggaa
      121 agtgaaatgc tagtggaaat ggtggtgcaa tccgaggaac gaagccggat cctgcgaaaa
      181 tgcatggaac atcttaacta cgactactgc gaggtattgt atttgcatta ttttgagaat
      241 atgagctatg cacaggcagc acaagttatg ggaaaaagca taaaacaaat cgatcatttg
      301 ctggagcgtg gtaagaaaag actccgctca ctattggaac gggagggaat taccgatgcg
      361 aaatactgat gaacggcttg ctgccgtaaa aaggcgtgtg gaagaattgg aacagaaaaa
      421 aaggcagctt agataccgtt atatcgccat gtcggctgtt gctgcttgct tggttatcat
      481 aataggcatg ggatttgcta tgcccggtat cattgcaggt ctttacggaa gggattatag
      541 taatacagga atgatggcaa gtatttttta tgagggtaaa gtccttggct atgtattgat
      601 tggaatattg tcttttgtgc tcggtgtgtg tctgactgtc ctttgttttc ttttaaggcc
      661 agaaaaacag cgggataagg aggaccacaa caatgataga actcattgac aattccgtac
      721 agcttgcagt tttgaccgtc tgctgcatct actccgctat acggtcaata aggcgtaatg
      781 atcatgtctg gtttctgctg acctgctttt atggagccta tgcccttggg ctggtttact
      841 ggttgttgtt cattgttttt cactcgaaga cgccaagtat ttctcccgta tcggatttaa
      901 gctggatggc aagcgtattg tttattctgg ttatgcaaaa caccttacga ttaccggggg
      961 agagtactta ccgacctatt ttggcatgga cagctcctgt cttcagcaca gttatgtgtt
     1021 tctttttttt ccattgggga gactatttcc tgaatgtttt atgggctgca cttatggggg
     1081 cttgcgggta tcaagctttg cgagggctgc tttgggcgag gcgacagagc aatgggatga
     1141 acagctccct atattttcat attacggttt tgacctttct tttggtggag tatagtcttt
     1201 ggcttgcttc ctgcttctgg aaaaccgaga gtcttcttaa cccgtattat tggtttgact
     1261 ttctcatgtc tgccacgttc tttacctttc ttccatctgt gaaaaaggtg gtgacagaat
     1321 gatctatatt gaaaatgtgt tcatctgcat tacagcacct ttactgattg ccatgctgtg
     1381 cggggatagg aacaaccggc ctgtttttat ttttttaatc tcaggaattg gcatgtgcct
     1441 tttatctgca tatattaata cctttgttgc cagctgttac ggtgtcgata tgatacaggc
     1501 aacggtggag cttgcaccgg cgattgagga aaccatgaag ctgcttcctg tgctgtttta
     1561 tttactggta ttggagccgg aacaaaggga ggcacaaagt gctattttct acgtggcggc
     1621 aggttttgca acctttgaaa atgtatgtta ccttgttgaa aacggcacat cacggatttt
     1681 atttttactt atgcgtggat ttggtgctgg tgcaatgcat attgtctgcg gggcaattgt
     1741 gggatacggg cttctgtacg tttggagtag atcatggcta aaggttgcag gaaccttcgg
     1801 acttttgtgc attgcaatca cctttcatgc catctacaat ttgcttctta gtgtgggggg
     1861 tactgcacag acagtaggtt tgcttatacc cgtccttact gtcttatccg gtataggaat
     1921 caccaagata ctgaggttca aatcatcaaa ataaaagctc ttggggcatc gagcccccat
     1981 cgctttttgg tcatttcact tcgtgaaatc ccactgacaa aagctcctgg ggcatcgagc
     2041 ccccatcgct ttttggtcat ttcacttcgt gaaatcccac taataatgag atttaaagaa
     2101 agtctgagca gcttaaaagc ggctcttttt ttactcctgc gaatgtgcaa ttatttttca
     2161 ccatctataa attagaatta ataaatcaac tttcgcacat agttaatgtg cagtgaacat
     2221 tgaaaaatca atagttttgg gcaataaaaa agttcaagaa acctcttc
//
LOCUS       DAOOJC010000399         2258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_37700_length_2258_cov_2.949160, whole genome shotgun sequence.
ACCESSION   DAOOJC010000399 DAOOJC010000000
VERSION     DAOOJC010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37700_length_2258_cov_2.949160"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLA01_09500"
     CDS             <1..782
                     /locus_tag="PLA01_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014522596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPD01566.1"
                     /translation="KENYRKLPKKVLRLLMIKRGDHPFIGQWALPGGFVNMNESLDEA
                     ALRELKEETNIDNIYMEQLYTWGDVDRDPRTRVISASYMSLVDSSTLDIKASDDADDA
                     KWFTVSCKMYQEQKTVTENGYVLQRWFKLTLSNDDDELYAIVKTEKTMEGKIASIKRE
                     IVQSHGIAFDHAKVIQYGIERLRNKIEYTDVAFNLMPSLFTLTELQQVYEIILDTELL
                     KANFRRKIADMVVETNEYTKDAGHRPSKLFRFNPDWGNTSG"
     gene            901..1491
                     /locus_tag="PLA01_09505"
     CDS             901..1491
                     /locus_tag="PLA01_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPD01567.1"
                     /translation="MFIVGLNGSPNKDGNTKFLLNTVLEEAKNLGAKTMIMEVQELLD
                     SCKNAFCVACSNPCSGVCYKGTKLEEAYETLKKADAIVMGSPVYFGSVSAQLKAFFDK
                     TRKLRGEKAFCNKIACGVTVGASKYGGQETTMKALHDIMLVHGMTVVGDGYIGDDCGH
                     HGVSAQRPAQEDEFALKRAGIIARRLVEACKEKGTE"
     gene            1537..>2258
                     /locus_tag="PLA01_09510"
     CDS             1537..>2258
                     /locus_tag="PLA01_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HPD01568.1"
                     /translation="MSGEFSRTELLIGRSSLEILKERKVAVFGIGGVGSFVVEGLARC
                     GLGSLVLIDNDFIVPSNINRQLHATSKTVGRPKVEVMRERILEINPEAEVTVHQEFYL
                     PETAEWLVSKDYDYIVDAIDTVTGKIGLVVQAKSMDIPIISSMGAGNKLDPTGFEVAD
                     IFETSVCPLAKVMRKELRERGIISLKVVYSKEEPIKMKQNPDTNENSNSNEEPEKSLN
                     KKRFVPGSISFVPSVVGLIIAG"
BASE COUNT          708 a          359 c          594 g          597 t
ORIGIN      
        1 aaaaggagaa ttacagaaag cttcccaaaa aggtcttgcg gctcttgatg ataaaaaggg
       61 gtgatcatcc gtttatcggg caatgggctc ttcccggcgg ttttgtgaat atgaatgaga
      121 gtctggatga ggctgcatta agggagttaa aagaggagac gaatattgac aacatttata
      181 tggagcagct ttatacatgg ggtgatgtag acagggatcc ccgtacaaga gtgataagtg
      241 cctcctacat gtccctggtg gacagttcaa ccttggatat taaggccagt gatgatgccg
      301 atgatgccaa atggtttacc gtgtcgtgca aaatgtatca ggagcaaaag acggttacgg
      361 agaacggata tgtattacaa cggtggttta aattaaccct gtccaatgat gacgatgaac
      421 tttatgcaat tgtgaaaacg gaaaagacta tggagggaaa aatagcaagt attaaaagag
      481 agattgttca atcccacggg attgcttttg accacgcaaa ggtcatacaa tacggaattg
      541 aaaggcttag aaataaaata gagtatacgg atgttgcatt taaccttatg cccagccttt
      601 ttaccctgac tgaacttcag caggtttatg agataatcct tgacacagag cttttaaagg
      661 ccaatttccg aaggaaaatt gccgatatgg tggttgagac caatgaatat acaaaggatg
      721 caggccacag gccttccaag ctgtttaggt tcaatcccga ctggggcaat acttcaggat
      781 aaatttttat atttgagaac ttttgggtaa tgtgttagta ttatatctta agaggattga
      841 attaaaaggg tttatttttc tttaccttta tatcaaaaca tttataagaa ggtgatttaa
      901 ttgtttattg tagggcttaa cggtagtccg aataaggatg gaaatacaaa gtttttattg
      961 aacactgtgc tggaagaagc aaagaatctg ggagccaaga cgatgataat ggaagttcag
     1021 gaacttttgg actcatgcaa aaacgcattt tgtgttgctt gcagcaatcc atgctccggt
     1081 gtttgttata agggcacgaa gctggaggag gcttatgaga cattgaaaaa ggcggatgcc
     1141 attgttatgg gtagtcctgt atacttcgga agtgtttcgg cacagctgaa ggctttcttt
     1201 gataagacca gaaagttaag gggagaaaag gctttctgca acaagatagc ctgcggagtt
     1261 actgtggggg cttccaaata cggcggacag gaaacaacca tgaaagcatt gcatgatata
     1321 atgctggttc atggaatgac cgttgtcggc gatggatata ttggggatga ttgcggtcat
     1381 cacggtgtat cggcacaaag accagcacag gaggatgagt ttgccctaaa acgtgccgga
     1441 ataattgcca ggcggttggt ggaagcctgt aaggaaaagg gaactgaata atggattgta
     1501 aggtaaaatt ttggataaac tgaacagggt gaatttatga gtggtgagtt ttctagaacg
     1561 gaattattaa taggccggtc atcactggag attcttaaag aaagaaaggt agcagtgttt
     1621 ggaattggcg gagtgggttc ttttgtggtt gaaggcttgg ccaggtgtgg attgggaagc
     1681 ctggttctga ttgataatga tttcatagta ccatctaata tcaacaggca gctccacgca
     1741 acaagcaaaa ctgtaggaag acccaaggtt gaggttatga gggagagaat cttggagata
     1801 aatccggagg cagaagtaac cgtacatcag gagttttatc ttcctgaaac ggcagaatgg
     1861 cttgtatcaa aggactatga ctatattgtg gatgcaattg acaccgttac agggaaaata
     1921 ggtcttgtgg ttcaggcaaa gagcatggac atcccaatta tcagcagcat gggagcaggc
     1981 aataaattgg acccaacagg atttgaggtt gcggatatct ttgagacatc ggtttgtcct
     2041 cttgcaaagg ttatgcgaaa ggagcttagg gagaggggaa ttatatctct caaagttgta
     2101 tattctaagg aagagccgat aaaaatgaag caaaacccgg ataccaatga aaactccaat
     2161 tccaacgagg aacctgaaaa gagtcttaac aaaaaaaggt ttgtaccggg aagcatttcc
     2221 tttgttccat cggtggtagg actcataatt gcaggcga
//
LOCUS       DAOOJC010000400         2256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_37746_length_2256_cov_2.870059, whole genome shotgun sequence.
ACCESSION   DAOOJC010000400 DAOOJC010000000
VERSION     DAOOJC010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37746_length_2256_cov_2.870059"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..978)
                     /locus_tag="PLA01_09515"
     CDS             complement(259..978)
                     /locus_tag="PLA01_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HPD01569.1"
                     /translation="MRFVVDTHTHTIASGHAYSTVQEMAREAAAKGIEMFAITDHGPA
                     MKGAPYLYHFGNLRIIPESLFGVRVIKGVEANIIDYSGNLDMPDGYLKRLDFVIASFH
                     DICIEPSSLEDNTQAMVNVLKNPYVDAIGHPGNTTFKVDIEKVVRAACENGKFIELNN
                     SSFLVRAGCEENCREFAIECKKQGVRIVCGSDAHISFDVGRFDRIYKLLDEVLMPEEL
                     IMNTSTDKFNEYFKQKKERRK"
     gene            complement(1066..2001)
                     /gene="hprK"
                     /locus_tag="PLA01_09520"
     CDS             complement(1066..2001)
                     /gene="hprK"
                     /locus_tag="PLA01_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HPD01570.1"
                     /translation="MYSVPLEQIIKDFSLECIAGKDRINDIVLTSTDVNRPGLQLVGY
                     LEHFGTDRVQIIGMVETTYLSGLSSEERYLRLDEFFKCGFPCMVVTRGLEVFPEMIAV
                     AEKYGTPILRTQDITSRFLSALIGYLNVELAPRTTKHGVLIEVYGEGILILGESGVGK
                     SETALELVKRGHRLVADDLVEVRRVSDKTLYGTAPDIIRHFIEIRGIGILDVKNLYGV
                     RSVKVTENINLVIQLEFWDEKKNYDRLGLVDEYIDILGIKVPCLTIPVRQGRNLAVIV
                     EVAAMNNRQKNMGYNAARVIKERMEDRMNNAGYEE"
BASE COUNT          692 a          555 c          318 g          691 t
ORIGIN      
        1 tgctttgtca ataccttcag ccccttacca atctatttta aagactcata acagtgcctt
       61 tcaccatagc cacatatctt atttagctgc actgataatg taaattgtgt tttcaacctt
      121 gcacaaggtt aacatgcaaa acatgatgta ttgtctattt tttctatttc cgggcactat
      181 tattcatact actattttac ttaagtatca ggctatatag aattaaatat gaactttatc
      241 ttcataccgt aagtttcttt attttctccg ttcctttttc tgtttaaagt actcattgaa
      301 cttatcggtg gaagtattca taattaattc ctcgggcatt aaaacttcat ccagaagctt
      361 gtatattcgg tcaaaacgtc ccacatcaaa gctgatatgg gcatcgctgc cacagacaat
      421 tcttactccc tgttttttac actcaatggc aaactctctg cagttctcct cacagcctgc
      481 tctaaccaaa aaggaactgt tgttaagctc tataaacttg ccattttcgc aggcagccct
      541 taccaccttc tcaatatcca ccttgaacgt ggtgtttccg ggatgcccga tagcatccac
      601 ataaggattt ttcaaaacat ttaccatagc ctgggtgtta tcctccaaag aagaaggttc
      661 aatacatata tcatgaaagc tggctataac aaaatcaagc ctcttcaaat atccgtcagg
      721 catatctaaa ttacccgaat aatcaattat atttgcctct acacccttta tgacccttac
      781 accgaataaa ctttcaggta taatcctgag gtttccgaaa tggtataagt atggtgctcc
      841 cttcattgca ggaccatgat ccgtgattgc aaacatttca attccctttg ccgcagcctc
      901 tctagccatc tcctgcactg tgctgtaggc atgcccactg gctatagtat gtgtatgagt
      961 gtccactacg aatctcaaaa ctattccccc atttacatgt aaatcatatt tattctatct
     1021 aaattcaaga ccttcctaag agacaaagtt caaaaaacta agaggctatt cttcatatcc
     1081 cgcattgttc attctgtctt ccattctctc ctttatgacc cttgcagcat tatatcccat
     1141 gttcttttgc ctgttattca tggctgcaac ctcgacaatt acggcaagat tacgtccttg
     1201 ccttacagga atagtaaggc agggaacttt tattcccaat atgtctatat actcatcgac
     1261 caaacccaac ctgtcgtaat ttttcttctc atcccagaac tcaagttgta ttacaaggtt
     1321 aatgttttca gtaaccttga ccgatctaac accatataga ttttttacat caagtattcc
     1381 aatacccctt atttcaataa aatgccttat tatatccgga gctgtaccat aaagggtttt
     1441 gtccgatact ctcctgacct caaccaaatc atctgccaca agccgatgac ctctttttac
     1501 aagctccagg gcagtttcac tttttcctac accactctct cccaatataa gtataccttc
     1561 accgtatact tcgattaaaa ccccatgttt tgtagttctt ggtgcaagct caacattaag
     1621 atacccaatc aacgcactca aaaaccttga ggttatatcc tgagtcctta aaataggagt
     1681 accgtatttc tccgctactg ctatcatttc agggaaaacc tcaagacctc tcgttacaac
     1741 catgcacggg aaaccgcatt taaaaaactc atccaaacgc aaatagcgtt cctcagagga
     1801 taaacccgaa agataagtag tttcaaccat tccaataatc tgaacacggt ccgttccaaa
     1861 atgctccaga taccccacca actgcaatcc cggcctgttg acatcggttg aagtcagcac
     1921 aatatcatta atcctgtctt tcccagcaat acattccagc gaaaaatcct ttataatttg
     1981 ctctaaaggc actgaataca taattatccc cccagccaca caaaaattat acaccaaaaa
     2041 aatatcacaa tttttaatta gaataacagc cttttatatt ataacacagt agaaaccata
     2101 tgataaacag taaataaaat ctgagaagag ttcaactttc tcgactcaat acagaatatt
     2161 ataagaacag aaaaaaacca ctggatataa acaaaactta atggagcggg tgaagggaat
     2221 cgaaccctcg caatcagctt ggaaggctga tgttct
//
LOCUS       DAOOJC010000401         2249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_37933_length_2249_cov_3.668642, whole genome shotgun sequence.
ACCESSION   DAOOJC010000401 DAOOJC010000000
VERSION     DAOOJC010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37933_length_2249_cov_3.668642"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2249)
                     /locus_tag="PLA01_09525"
     CDS             complement(<1..>2249)
                     /locus_tag="PLA01_09525"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPD01571.1"
                     /translation="ANILTLTFSRASARDMQDRFYSVFGRELICNVQFSTIHSFCYSV
                     IRDYTAANNRPFPKIIEGNESTVNKSQILRQIYFEINKDYITEDILEDLVNSISFIKN
                     MLYTPDKIGELDVKIKNFVEIYNAYEKLKRNERYIDYDDMLTGTYQLFMKDTGVINSL
                     RKRYRYINVDESQDTSFVQHEIIRLLAYPKNNIFMVGDEDQSIYGFRAAFPEALLKFK
                     ETYPEARILMMERNYRSTKNIVKAANNFIKQNKERYEKEMFTENEAGNEVVFKQVTDR
                     NEQYAYLASVLKMQDNLSECAVLFRNNISAIALVDYLEYSNIPFYIRDTNIHFFNHWV
                     VNDILAFIRFALNPHDIEAFAQIYYKTDAYLSKDMLEFVMVNQNRDKTVLQTLLDFPQ
                     LPKRTEEAVKSLIKRFGTIEFLKPHLAIEYIEKDLGYEKYIGRASKEMGFSAESINYI
                     IDSLKTIASRVNSFKDLFERLSVLNTAMENAVANRNRNAVVLSTIHSSKGLEFERVYM
                     IDLVEGQFPSAKSISELEEGNRSLMEEEVRLFYVGITRAKKHLELITFSQANNKKSFV
                     SRFVHQLKLAQKQFSCNEICEGAWVEHLKFGQGLVTRIDEVKDLIVIECKKAGVKMLS
                     LRVCMEEGKIKPSDSSECFETVFPDDKKVLLGLSVGELRELALRIGKNGKEKEILPLL
                     FVHYDYEVRRRACSAAAKLKDAEITAHIKPCIYAKEPQIRQYALKAILKSRCRELVED
                     VKKVLETED"
BASE COUNT          631 a          517 c          351 g          750 t
ORIGIN      
        1 ttatcctccg tttccaacac cttttttaca tcctcaacca gctctctgca cctgctcttt
       61 agtatcgcct taagtgcgta ttgccgaatc tgaggctctt ttgcatatat acacggctta
      121 atatgagcag taatttctgc atccttgagt ttcgctgctg cagaacatgc acgccttctt
      181 acttcatagt cataatgcac aaacaacaag ggaagaatct ctttttcttt tccgtttttc
      241 ccaatcctca aggcaagctc tcttaactcc ccaacgctta atcccaacag tactttttta
      301 tcatcaggaa acaccgtttc aaaacattca ctgctgtcgc taggtttgat cttcccttcc
      361 tccatacaaa ctctaaggga cagcatcttt actcctgctt ttttacattc tattacaatc
      421 aagtccttaa cctcatcaat tctggtaact aaaccctgtc cgaatttcaa atgttcaacc
      481 catgcccctt cgcatatttc attgcatgaa aactgctttt gtgctagttt tagttgatgg
      541 acaaaccttg acacaaaaga tttcttgtta tttgcctgag aaaaggtaat gagttcaaga
      601 tgctttttgg cccttgtaat gcccacataa aaaagcctca cctcttcctc cattaaagaa
      661 cggtttccct cctccaactc acttatgctt tttgctgacg ggaactgtcc ttccaccagg
      721 tcaatcatat aaactctttc aaattccaaa cctttacttg agtgtatggt ggatagcaca
      781 acagcatttc tgtttctgtt cgccacagcg ttttccatag cagtattcaa tacggaaagc
      841 ctctcaaaaa gatctttaaa ggagttaacc cttgatgcta ttgtctttag actgtcaata
      901 atataattaa tgctctctgc ggaaaagccc atctccttcg atgctcttcc aatatacttt
      961 tcatatccta aatccttctc aatatactca atcgccaaat gtggctttag aaactcaatg
     1021 gttccaaatc gcttaatgag ggatttaacg gcttcttccg tcctttttgg caactgcgga
     1081 aaatccagca gggtttgtag aaccgtttta tcccggttct gatttaccat aacaaactca
     1141 agcatatcct tgctaagata tgcatcggtt ttataataaa tctgtgcaaa ggcttcaatg
     1201 tcatggggat ttaaagcaaa ccttataaaa gcaaggatat cattaaccac ccagtggttg
     1261 aaaaaatgaa tattggtatc ccttatataa aagggaatat tgctgtattc caggtaatcc
     1321 accagtgcaa tggcagaaat gttgttcctg aataaaacgg cacattcgga cagattgtcc
     1381 tgcatcttca gaaccgaagc cagataagca tattgttcat tcctatccgt tacctgctta
     1441 aataccacct cattccctgc ttcattttcg gtaaacatct ctttttcata ccgttccttg
     1501 ttctgcttta taaaattatt ggctgccttc acaatatttt ttgttgaacg gtagtttctt
     1561 tccatcataa gaatccttgc ctcgggatag gtctctttaa acttgagaag tgcctcggga
     1621 aatgcggctc taaacccata aatactctgg tcctcatccc ccaccataaa aatgttgttt
     1681 tttggatagg caagcagcct gataatttcg tgctgtacaa aggaagtatc ctgggattca
     1741 tcaacattta tataccgata tcttttcctc aacgaattga taaccccggt atctttcata
     1801 aaaagctgat aggtaccggt taacatatcg tcataatcta tatatctttc attgcgtttt
     1861 agcttttcat aggcattata tatttcaaca aaattcttga tttttacatc caattcccct
     1921 atcttgtcgg gagtataaag catattttta ataaagctga tggaattgac cagatcttcg
     1981 aggatatctt ctgttatata gtccttgttt atttcaaaat aaatctgcct taaaatttgg
     2041 cttttattaa cagtgctttc attaccctca atgattttag gaaaaggtct gttattagct
     2101 gcagtataat cccgtataac cgaatagcaa aagctgtgta ttgttgaaaa ctggacatta
     2161 cagatgagtt ctcttccaaa aacactgtaa aacctatcct gcatatcacg ggcagatgcc
     2221 ctgctgaatg tcagtgtcaa tatgtttgc
//
LOCUS       DAOOJC010000402         2245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_38018_length_2245_cov_3.354338, whole genome shotgun sequence.
ACCESSION   DAOOJC010000402 DAOOJC010000000
VERSION     DAOOJC010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38018_length_2245_cov_3.354338"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PLA01_09530"
     CDS             complement(<1..518)
                     /locus_tag="PLA01_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030248 - cellulose binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose binding domain-containing protein"
                     /protein_id="HPD01572.1"
                     /translation="MIPSVFYIFSEKSIGSNNHGNVTFPPEATVSLVQQTPSVAVDGT
                     KPPITASGKTETPLPTETVTPTITPTPTPTATPTATPTATPTATPTLTPTPTPTLTPT
                     PTRTPTPRPAPTSVPKPTEAGPKKKDNITVQYKNASYVSSASSIAPVFRITNQGSSDV
                     KLSDIEIRYYYT"
     gene            complement(824..2227)
                     /locus_tag="PLA01_09535"
     CDS             complement(824..2227)
                     /locus_tag="PLA01_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HPD01573.1"
                     /translation="MNIEVYGNGLSDPPKRIVGYFADWNIYLEDNYYEVSDIPWDKIT
                     HINYAFAKIENGKIAIYDKWAAIDKPFGDDTWETPLKGHFGQLIKYKKQYPQVKTLIS
                     VGGWTLSTYFSDVALTEESRSVFADSCVEFVRMYQFDGVDIDWEYPVGGGMSTNVARP
                     EDKQNFTLLLKCLREKLDAAGEEDGKRYLLTIAAPAGSYTIKNTEPELYHQYLDFINL
                     MTYDYSGSWDTSVNHMSPLYMNPDDPSSSQRKETFNADWTVNEYLRLGVPAEKLNLGV
                     PFYAAGWKNITGGTNGLFGTSGQALNNEMYHFIRGLLDSTTSGFARYWDDNSKVPYVW
                     NPVSATLYTYEDEISLENKCNYVLNKGLGGIMIWELSGDYPANGGSTLTSIIHSTFSR
                     DLPEDDKYGDVDADGDVNSTDITILKRYLLRKMDRLPYPEREYLIDLNMDGIVDSTDL
                     TILKRYILRKLDVIPIN"
BASE COUNT          658 a          473 c          395 g          719 t
ORIGIN      
        1 tttgtatagt aatacctgat ttcaatatcc gatagcttta catctgagct gccttgattg
       61 gttattctga aaacaggtgc aatactgctg gctgacgata cataactggc gtttttatat
      121 tgaaccgtga tattgtcttt tttcttagga cctgcttccg tcggttttgg cacagatgtc
      181 ggtgcaggtc tcggcgttgg tgtccgtgtt ggggtcgggg tcaacgtagg tgtaggcgta
      241 ggtgtaagtg taggcgtagc tgtaggtgtc gctgtaggtg tagctgtagg tgtagctgtt
      301 ggcgtaggag tcggcgtaat tgttggggta actgtttctg tcggcaaagg agtttctgtt
      361 tttccggaag cagtaatagg tggcttcgtg ccatcaacag caactgatgg tgtttgctgc
      421 accaaggaaa cagtcgcctc aggaggaaat gtcacatttc catggttgtt cgaacctata
      481 ctcttctctg aaaaaatata aaaaactgaa ggaatcacta taacacatat aaataatgca
      541 atcgctgaga ttattataac ctgtttttta agtcttgatt tataatacca tttcataatt
      601 tagcatgtat ataaagtggc cattgaatta aacatccagc aagccttata tacaatttaa
      661 ccctccctaa atagcatata gaaatctgga actaaataat ctatttctta ctataatcat
      721 tcaactacta aaaagcaacc caattatcat ttattcttgc tataatatcc aataacgcat
      781 aaatacctga atgatattat aacagtacta atattaatat atgttaattt attggaatta
      841 cgtctaattt tcttagtata tatctcttca aaatagtcag gtccgttgaa tcaacaatcc
      901 catccatatt taaatcaatc agatactctc gttccggata gggtaatctg tccattttac
      961 gcaacaagta ccgctttaat attgtaatat ctgttgagtt tacatctcca tcggcatcta
     1021 catcaccata cttatcatcc tcaggtaagt ctctagaaaa tgtgctgtga atgatagatg
     1081 tcagagtact tcccccatta gccggatagt caccgctcag ttcccatatc ataatacctc
     1141 caaggccttt attcaaaaca taattacact tgttttccaa agagatttca tcttcatatg
     1201 tatacaatgt ggccgatacc ggattccaca cataaggaac tttagaatta tcatcccaat
     1261 atctggcgaa tcccgatgtc gttgagtcca acaatcccct tataaaatga tacatctcat
     1321 tattgagtgc ctgtcccgat gtcccaaaaa gtccattcgt tcctccggtt atatttttcc
     1381 aaccggctgc atagaatgga acacctaaat tcaacttttc tgcgggaaca ccgagcctta
     1441 agtactcatt aacagtccaa tccgcattaa aagtctcttt cctttgagaa gatgatgggt
     1501 catcaggatt catatacaaa ggcgacatat ggtttacgga agtgtcccac gaaccgctgt
     1561 agtcatatgt cataagattg ataaaatcaa ggtattgatg atacagttca ggctctgtat
     1621 tttttatcgt atagcttcca gccggtgcag ctatagtcag aagatacctc tttccatctt
     1681 cttctcccgc tgcatcaagc ttttcacgca aacatttgag aagcagggta aaattctgct
     1741 tgtcttccgg ccttgcaaca ttagtggaca ttcctccgcc tacaggatac tcccagtcaa
     1801 tatccacgcc atcaaactga tacatacgaa caaattcaac acaactgtca gcaaatacag
     1861 atcttgattc ctccgtaagt gcaacatccg aaaaatatgt agacagagtc catcccccaa
     1921 cagatattaa tgtctttact tgcggatact gtttcttata ctttatcagt tgtccgaaat
     1981 ggccttttaa aggtgtttcc caagtatcat ctccaaaagg cttatcaata gcagcccatt
     2041 tatcatatat tgcgatcttt ccattttcaa tttttgcaaa agcataattt atgtgcgtta
     2101 tcttatccca tggaatgtct gaaacttcgt aataattgtc ttctaaatat atattccaat
     2161 cggcgaagta accgacaatc ctttttgggg gatcagataa accgtttcca taaacctcga
     2221 tattcattaa cgttagaatt actga
//
LOCUS       DAOOJC010000403         2245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_38020_length_2245_cov_3.088584, whole genome shotgun sequence.
ACCESSION   DAOOJC010000403 DAOOJC010000000
VERSION     DAOOJC010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38020_length_2245_cov_3.088584"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLA01_09540"
     CDS             complement(<1..326)
                     /locus_tag="PLA01_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HPD01574.1"
                     /translation="MSFKIGDIVARKSYGSDILFKVVDIKNEGGKRVAILKGICYRLE
                     ADAPESDLMVQPESYVREYNARINMSVDEKVRSITASALRTNTKKKIFIAQFLKRKIR
                     AFQNLAG"
     gene            486..2156
                     /locus_tag="PLA01_09545"
     CDS             486..2156
                     /locus_tag="PLA01_09545"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPD01575.1"
                     /translation="MLTDIEIAQLCKMKPINQIASELGIKDDELELYGKYKAKLTDDL
                     WERVKDTPDGKLVLVTAINPTPAGEGKTTTTVGLGQAMAKIGKKAIIALREPSLGPVM
                     GIKGGAAGGGYSQVVPMEDINLHFTGDMHAITAANNLLSAAIDNHIQQGNALGIDPRQ
                     IIWKRAMDMNDRALRNIVVGLGGKINGTPREDGFLITVASEIMAILCLSTGIMDLKDR
                     LGKIIIGYTYGGEPVSARDLKVNGAMTLLLKDAIKPNLVQTLENTPALMHGGPFANIA
                     HGCNSIIATRLGLKLADYCITEAGFGADLGAEKFLNIKCRYGGLKPDAVVLVATIRAL
                     KYNGGVKKDMLAAPDLDALKKGFVNLEKHIENIRKFGVPVIVAINHFSTDSDEETDFI
                     KNKCESMNVEVSFSNVFAMGGEGGRELAEKLVKLIENQKNDFKHLYDTNMTIKQKIET
                     IAKEIYGARGVVYLPAAENAIKKIESLNMDKLPICMAKTQYSLSDNPSLLGRPEGFDI
                     TVREIKLSAGAGFIVAITGDIMTMPGLPKTPAAENIDIDEKGVITGLF"
BASE COUNT          705 a          462 c          480 g          598 t
ORIGIN      
        1 cctgccaggt tttgaaaagc tcttatcttc ctctttagaa attgtgcgat aaaaattttt
       61 tttttggtat ttgttcttaa tgctgatgcg gttatactac gtaccttttc atccaccgac
      121 atattgattc ttgcattata ttccctgaca tagctttccg gttgtaccat aaggtctgat
      181 tcaggtgcgt cagcctccaa cctgtaacag attcctttca gtattgccac cctttttcct
      241 ccttcatttt ttatatccac aaccttaaac aatatatcgg aaccatagga ttttcttgct
      301 acaatatctc caattttgaa actcataaat accaccctta tttttatttt ccctacaata
      361 cataatatga tttaaagggt acgtttgtga gaggtattga aatactatgc caacaaacgg
      421 tataatgaat tatattgcaa aggtgtcctt attactgtct gacttttata cgaggaggga
      481 aaaccatgtt aactgatatt gagatagccc aattgtgcaa aatgaagcca ataaatcaaa
      541 ttgcctcaga acttggcatt aaggatgacg aacttgagct ttacggtaaa tataaagcaa
      601 aactgaccga tgatctgtgg gaaagggtaa aggacacgcc cgacggtaaa ttggttcttg
      661 ttacggcaat aaaccccacc cctgcaggcg aaggaaaaac cacaaccacc gtcgggcttg
      721 gtcaggcaat ggctaagata gggaagaaag caataatcgc tttgagagaa ccttctttag
      781 gtccggtcat gggaataaaa ggcggtgctg ccggaggcgg atattcccag gtagtaccca
      841 tggaagatat aaacttacac ttcacaggag atatgcatgc cataacagcg gcaaacaacc
      901 tcctctctgc tgcaattgac aaccatattc agcagggcaa tgcactcggt attgatccaa
      961 ggcagataat ctggaaaagg gcaatggaca tgaatgaccg tgccttaaga aatatcgttg
     1021 taggcctggg aggcaaaatt aacggcactc caagggaaga cggcttttta ataaccgtgg
     1081 cctcggagat catggctatc ctctgtctgt ctacaggaat tatggattta aaagaccgcc
     1141 tcggcaaaat tataattgga tacacctacg gcggagaacc cgtttctgcc cgggacctca
     1201 aggttaacgg ggcaatgacc ctcttattaa aggatgccat aaagcccaac ctggtacaaa
     1261 cccttgaaaa cacgcccgca cttatgcatg gcggtccttt tgccaacata gcccacggat
     1321 gcaacagcat tatagccaca aggcttgggc tgaagcttgc cgattattgc attacagagg
     1381 ctggtttcgg tgcagattta ggtgctgaaa agtttttaaa catcaaatgc aggtatgggg
     1441 ggctaaagcc ggacgctgta gttcttgtgg ctacaataag agctttaaaa tacaacggtg
     1501 gtgtaaagaa agacatgctg gctgcccctg atttagacgc cctgaaaaag ggatttgtaa
     1561 accttgaaaa gcatatagaa aatatcagaa agttcggggt ccctgttatt gtggccatta
     1621 atcatttttc caccgattcg gatgaagaaa cggatttcat taaaaacaaa tgcgaaagta
     1681 tgaacgtcga ggtatccttc tccaatgtat tcgccatggg aggagaaggc ggccgggagc
     1741 ttgccgaaaa gctggttaag ctaattgaaa atcaaaagaa cgattttaaa catctttatg
     1801 atacaaatat gacaataaag caaaaaatag aaacaattgc aaaggaaata tacggagcaa
     1861 gaggtgttgt atatttaccg gcagcagaaa acgccatcaa aaaaatcgag tcccttaata
     1921 tggataagct tcccatttgc atggctaaaa cgcaatattc attatccgac aatccttcgc
     1981 ttctcggaag gccggaagga ttcgatataa cggtgagaga aattaagctc tctgccggtg
     2041 caggatttat cgtagcaatc acaggtgata tcatgactat gcccgggctt ccgaagaccc
     2101 cggcagcaga gaatattgac atagatgaaa aaggtgttat aaccggattg ttttaacagc
     2161 aaagccctga tagacggctt acaaagatgc aaattttatc acctttttta ttctggcgat
     2221 ctaaagaaaa aataattctc attat
//
LOCUS       DAOOJC010000404         2244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_38049_length_2244_cov_2.876199, whole genome shotgun sequence.
ACCESSION   DAOOJC010000404 DAOOJC010000000
VERSION     DAOOJC010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38049_length_2244_cov_2.876199"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLA01_09550"
     CDS             complement(<1..567)
                     /locus_tag="PLA01_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD01576.1"
                     /translation="MTSDMLIKQYCKELRIGMNIYNSYSKIIATDHADFLAQLLKIEL
                     DHRELVRKNRNLKSAGFDVIKTFENYEFQDIQIPKAISIEELKTGIFIDRLENLILYG
                     PVGTGKSHMATAIGVAACSRGKKVKFYRTATLVNLLSEAKAKGELQRFMKQLQKIDLL
                     ICDEWGYVPFEKDGSQLLFQVISECYEKR"
     gene            complement(564..2072)
                     /gene="istA"
                     /locus_tag="PLA01_09555"
     CDS             complement(564..2072)
                     /gene="istA"
                     /locus_tag="PLA01_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HPD01577.1"
                     /translation="MLTMEQVYRIRNMKKFEGKSLRKISRITGHDFETVKKYVEKEDF
                     NQTLRPKQRRQGKLSPYRSIVTAWLINDKKAPHKQQHTAKRVYDRLKELYGAEFDASE
                     RSVRKLVAEIRKGLEMDTNGFLPLEHPPGEAQADFGEARFIENGIAYDGYYLNIAYPH
                     SNGGHMQLFKSMNQECLLEGMKAIFEHIGGVPTAIWFDNMSTIVKKIKEYGERDLTKG
                     FLRFMMHYGFQSNFCNPNSGHEKGSVENKVGYHRRNMLVPIPEFKDLKEYNKELLLRC
                     DRDMNRMHYKGYGLIKDLFQEDKQEFLKLPKVPFEVYIHEFAKTDNYGKVKFDGKIYS
                     TSPNMAKRQVIVKAGAFDVEILDEDCNHIIRHKRLYGEEKESMNWIPYLALMSKRPTA
                     LKYTGLYSQLPQILKEYLDKSDYERKKQTLKLFAKMTATTGIDTAIEAFEEGLKLGVS
                     DPDSIWATYCRLTTGTLPEPEIPLPDKVPELKKYIPDIKIYDQLITSGGLQL"
BASE COUNT          591 a          499 c          374 g          780 t
ORIGIN      
        1 ccttttctca tagcattctg aaataacctg aaacaaaagc tgtgagccat ctttctcaaa
       61 gggaacatag ccccattcgt cacagatcag cagatcaatt ttttgcaact gtttcatgaa
      121 tcgttgcaat tcccccttag cctttgcctc gctcaaaaga ttaactaatg ttgccgtacg
      181 atagaatttt accttctttc ctctggaaca agctgcaacc ccaatggccg tggccatatg
      241 gctcttaccg gttcctacag gtccataaag aatcaggttt tcaagcctgt caataaaaat
      301 tccggttttt agttcttcaa tactaatagc ttttggtatc tgaatatcct gaaattcata
      361 gttttcaaag gtctttatga catcaaagcc tgcagatttt agattcctgt tttttctgac
      421 aagttcccga tggtctaatt ctatctttag aagctgtgca agaaaatcag catgatcagt
      481 tgcgataatc ttcgaatagc tattgtagat attcatacca atccggagtt ccttgcaata
      541 ctgcttaatc aacatatcag aagtcatagc tgcagccctc ctgaagtaat cagttgatca
      601 taaatcttta tgtcgggtat atatttctta agttccggaa ctttatcagg caatggtatt
      661 tccggttccg gtaaagttcc ggtcgttaaa cgacaatatg ttgcccaaat actatcagga
      721 tcgctaaccc ctaattttag cccttcttca aacgcttcta tggctgtgtc aatacctgtg
      781 gttgctgtca tcttggcaaa caactttaaa gtctgctttt ttctctcata atcactttta
      841 tccagatatt ctttaaggat ttgtggcaat tgactgtata gtcctgtata ttttaatgca
      901 gtcggtcttt tgctcataag tgcaagatag ggaatccagt tcattgattc cttttcttct
      961 ccgtaaagcc gtttgtgtct gatgatgtga ttgcagtctt catcaagtat ttctacatca
     1021 aaagcacctg ctttaacaat gacctgtcgc ttggccatat taggagaagt tgaataaatt
     1081 ttgccgtcaa acttaacttt tccgtagttg tcagttttag cgaattcatg tatgtacacc
     1141 tcaaaaggaa ctttcggaag tttcaagaat tcctgtttat cttcctgaaa taaatctttg
     1201 attagcccat atcccttgta atgcattcta ttcatatccc ggtcacatct taaaagaagc
     1261 tctttgttgt attcctttaa atccttaaat tctggaattg gtacaagcat attccggcga
     1321 tgatatccaa ccttgttttc cacagaacct ttttcatgtc cactgttagg attacagaaa
     1381 ttgctctgaa aaccataatg catcatgaat cttaaaaatc ctttggttaa atcccgttct
     1441 ccatactcct tgattttctt aacaatcgta gacatgttgt caaaccatat ggctgtcgga
     1501 acaccgccga tgtgttcaaa gattgctttc atgccttcca acaagcattc ctggttcata
     1561 cttttgaaca gttgcatgtg accgccatta ctgtgaggat aagcaatatt taagtaatat
     1621 ccgtcatatg caataccgtt ctcgataaac cgagcctctc caaagtctgc ctgcgcttct
     1681 cccggaggat gttccaacgg aagaaacccg ttcgtatcca tctccaatcc ctttcgaatt
     1741 tccgcaacta atttcctgac tgatctctca ctggcatcaa attcagcacc atacagctct
     1801 tttaaccggt catacaccct ttttgcagtg tgctgctgtt tatgtggtgc tttcttgtcg
     1861 ttgataagcc atgcagtgac tatgctccta tatggagata acttgccttg tctcctttgt
     1921 ttaggacgga gtgtctgatt aaagtcttct ttctccacat actttttaac agtttcaaag
     1981 tcgtgcccgg taattcgaga tattttcctt aaactcttgc cttcaaattt cttcatgttt
     2041 ctgatacgat atacttgctc cattgttagc atcctttctt tacccccttc aagtatggac
     2101 aacttaaagg gtattatttt tcaggggtgc ccgcaatgga gtttccaatt tatgcaggga
     2161 tttttaagtt ccggaacgct cggtttttat tgtaccaaaa acaaatattc ttatatatat
     2221 cgttattatc agtattatta atct
//
LOCUS       DAOOJC010000405         2243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_38063_length_2243_cov_4.208867, whole genome shotgun sequence.
ACCESSION   DAOOJC010000405 DAOOJC010000000
VERSION     DAOOJC010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38063_length_2243_cov_4.208867"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2243
                     /locus_tag="PLA01_09560"
     CDS             <1..>2243
                     /locus_tag="PLA01_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPD01578.1"
                     /translation="EVLTLKSPDGTVIDIVTLINLSDDEAYGRKSDGSSEMSLLIPTP
                     GGANIFNQSMVPVLEPVFSHEGGLYTNEFDLKIAAQESKVKIYYTTDCSDPVPGETGT
                     YEYSTDIRIKSRKGDPNVLSMITDISNDEWNRWKAPKGELFKCTTIKAVAVRDDGAQS
                     KVVTHSYFVDPDMNNRYTLPVVSLVTDYNNLFDKTTGLYVNENYENSGVEWERPAHVE
                     IFDTDGSTWFSQSIGIRIHGGWTRKYPQKSFRLYADHGDDEIDKFKYEIFPGLRGNGT
                     GKKIKSFERLILRNAGNDWTGALFRDEMMQSLVSHLKLDTQSYRPCIVFLNGEYWGIY
                     HIRERFDEKYLEEHYDLDDDRVSILDVFEKPSVQEGDPEDALAYSEDVINYLKSHSIT
                     DKSTYDYIKTKIDVDNFIDYNVAEIFFGNTDWPGNNVSIWRYKTESGEYEPDAPYGQD
                     GRWRWFLKDTDFGFGLYEKPVSHNTLAFAAGDTKEGYANEPWAVFLFKTLLQNDEFRN
                     EFINRFADQLNTSFVPSRVNEIIDGVVSTLEPEMQEHTDRWPFIKLTRTSYMDKTWSE
                     EIQSIKNYANYRPGFVRQHITNKFRANGVTGTSYVTLNTDTAMGHIKINSIDIKSGTP
                     AVTDPGSWTGMYFMGVPVTVKAIPKDGYVFDHWAGINGVDQSSDTLTFTLTGDVNVTA
                     VFKPMLEPPTLYGDVNADGEINSTDLTYLKRYLLKTLSSGLYFDLKAADVDASGSIDS
                     TDLTYLK"
BASE COUNT          708 a          398 c          524 g          613 t
ORIGIN      
        1 gagaagtact gacgttaaag agcccggacg gaacggttat tgatattgtg actttaataa
       61 atctttcgga tgatgaggcc tatggaagaa aaagtgatgg ctcatcggaa atgtcattgt
      121 taataccaac tcccggaggg gcaaatattt ttaaccaaag tatggtgcct gtgttagaac
      181 cagtgttttc ccatgaaggt gggctttata ctaatgagtt tgatttgaaa attgctgcac
      241 aagaatcgaa agtgaaaatt tactatacta cagattgttc tgatcctgtt ccgggggaaa
      301 ctggaaccta tgaatacagt acggatatta gaataaaaag caggaaaggt gatcccaatg
      361 tcctttcaat gattaccgat atatccaacg atgaatggaa cagatggaaa gcaccaaagg
      421 gtgaattatt taaatgtacc accattaaag ctgttgctgt aagagatgac ggtgcacaaa
      481 gcaaggtagt cactcattcc tattttgtag atccggatat gaataaccgg tacaccttgc
      541 cggtggtttc tctggtaacc gattataata atttgtttga taagactaca gggctttatg
      601 taaatgaaaa ctatgaaaat agcggagttg aatgggaaag acctgctcat gtagaaatat
      661 ttgataccga cggaagcacg tggttttctc aatcgattgg gatacgcata catgggggat
      721 ggacaagaaa gtatcctcaa aagtccttcc gcttatatgc agatcatggc gatgatgaga
      781 ttgataaatt caaatacgaa atattccccg gccttagagg aaatggaacc ggcaaaaaaa
      841 taaagagctt tgaacgtctc atattaagaa atgcaggaaa tgactggact ggtgctttgt
      901 tcagggatga aatgatgcag agcctcgtat ctcatctaaa gctggatacc caatcttaca
      961 ggccttgcat agtatttttg aacggcgagt actggggaat ataccatata cgtgaacggt
     1021 tcgatgagaa atatctcgaa gagcattacg acctggatga cgatagggtt tcaattcttg
     1081 atgtttttga aaaacccagt gttcaggaag gagatccgga ggatgctttg gcatattcgg
     1141 aagatgttat aaactatttg aagtctcatt ccattacaga taagagtaca tatgattata
     1201 ttaagacaaa aatagatgtt gataatttca ttgattacaa tgtcgccgaa atattcttcg
     1261 gaaacacgga ctggcctgga aacaatgtga gtatatggag gtacaaaacc gaaagcggtg
     1321 agtatgagcc cgatgcaccc tacggtcagg acggaaggtg gagatggttt ttaaaagaca
     1381 ccgattttgg ctttggcttg tatgaaaagc ccgtgtccca taacactctg gcttttgcgg
     1441 ccggtgacac taaagaaggc tatgcaaacg aaccgtgggc ggtatttctg tttaagaccc
     1501 tgctccagaa tgatgagttt agaaacgagt ttataaaccg ttttgcagac cagttgaata
     1561 cttcttttgt tccgtcaaga gtaaatgaga ttattgatgg agttgtatcg acccttgagc
     1621 cggaaatgca ggaacacact gaccgctggc cctttatcaa gctgacaaga accagctata
     1681 tggataagac atggagcgag gaaattcaga gtataaaaaa ctatgccaat tatcgtcctg
     1741 ggtttgtaag acagcacata accaacaagt ttagagccaa cggtgtgacc ggaacatctt
     1801 atgtcaccct gaacaccgat acagccatgg gacatattaa gattaattcc atagatatta
     1861 agtcgggtac tccggcagtt acagaccccg gttcatggac tggtatgtat ttcatggggg
     1921 ttcctgtaac tgtaaaggca ataccgaaag atggctacgt gtttgaccat tgggcgggaa
     1981 taaacggggt tgatcagtca tcggataccc taacctttac cctaaccggg gatgtcaatg
     2041 ttacggctgt tttcaaaccc atgttagaac caccaacttt atatggtgat gttaatgcag
     2101 acggggaaat aaattctacg gatttaacct accttaaaag atatttgcta aaaaccttgt
     2161 cttccggttt atatttcgat ttaaaagcag cagatgttga tgccagtgga agtattgatt
     2221 caacggatct tacgtatttg aaa
//
LOCUS       DAOOJC010000406         2235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_38285_length_2235_cov_2.666514, whole genome shotgun sequence.
ACCESSION   DAOOJC010000406 DAOOJC010000000
VERSION     DAOOJC010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38285_length_2235_cov_2.666514"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /gene="dapA"
                     /locus_tag="PLA01_09565"
     CDS             complement(<1..642)
                     /gene="dapA"
                     /locus_tag="PLA01_09565"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPD01579.1"
                     /translation="MRKPIFTGAGVAIITPFTEDGIDFNKLGELIDFQINEGIDSIII
                     CGTTGEASTMPDDEHKSAIKFAVEKVNKRVPVIAGTGSNDTKHAISLSKYAEEVGADA
                     ILSVTPYYNKTTQKGLYEHFRLIANSIKIPMILYNVPGRTNLNIDPQTVKQLSEIENI
                     VAIKECNLSQVGEVINLCPEDFTVYSGNDDQVLPLLALGGKGVISVMANIIPKK"
     gene            complement(773..1771)
                     /locus_tag="PLA01_09570"
     CDS             complement(773..1771)
                     /locus_tag="PLA01_09570"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPD01580.1"
                     /translation="MKKINLAVVGATGMVGRTMIKVLEERNLPIENIYFFSSSRSAGT
                     AITFNGKEYTVEELTETSFDRGIDIALFSAGAGTSKEFAPIAASKGCVVIDNSSQWRM
                     DETVPLVVPEVNPQDISWNKGIIANPNCSTIQAVVALKPLHDKYKIKRIVYSTYQAVS
                     GAGMKGYADLENGLKGEAPQKFPHPIAGNCLPHIDVFLPNGYTKEEMKMVNETRKILG
                     DQSLRITATTVRVPVFHSHSESINVEFEKQFDLNELINVLKNAPGIVVEDDVANNIYP
                     MPINAEGKDETFVGRIRRDESVESGVNLWVVADNIRKGAATNAVQIAQELIKMWNK"
     regulatory      complement(1936..2112)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
BASE COUNT          598 a          470 c          398 g          769 t
ORIGIN      
        1 ctttttggga attatatttg ccataaccga tattactcct ttaccgccta aggcaagcaa
       61 aggaaggacc tgatcatcat tacccgaata aactgtgaag tcctccggac ataagttaat
      121 tacttctcca acctgactta agttgcattc cttaattgca acaatatttt ctatttcaga
      181 caattgcttt acagtctgag ggtctatgtt aaggtttgtt ctgcctggta cattatataa
      241 aatcatgggt atttttatgc tgtttgcaat cagccggaaa tgttcgtaaa gacctttttg
      301 ggttgtcttg ttgtagtagg gggttacgct taatatggcg tctgcaccta cttcttcggc
      361 atacttgctt aaacttatag catgtttggt atcgttgctc ccggttcctg caattacagg
      421 taccctcttg tttaccttct ccacagcaaa ctttatggcc gacttatgct catcgtccgg
      481 cattgtcgat gcttccccgg tggttccgca gataatgatc gaatctatac cttcgtttat
      541 ttgaaaatcg atgagctctc cgagtttatt aaagtcaatg ccgtcttcgg taaatggtgt
      601 tattatagct actccagcac cggtgaatat tggttttctc ataataacca tcctttcact
      661 tataaaattc caagacttaa acctcattag tgtggattat tccattgagc ttcagtgggg
      721 agtagaatcc accactgaaa ccgtaaatta caagctttag caaaagtgtt ggctatttgt
      781 tccacatctt tatgagctct tgtgcaattt gaacagcatt tgtagcagca ccttttctta
      841 tgttatcagc aactacccat aagtttacgc cgctttcaac gctttcgtcc cgtcttattc
      901 ttcccacgaa ggtctcatct tttccttccg catttatagg cataggatag atgttgttgg
      961 caacatcatc ttcaactacg atgcccggag catttttcag aacgtttatc aattcattta
     1021 aatcaaactg tttttcaaat tcaacattta tcgactcgct gtgactgtgg aaaacaggta
     1081 ccctgacggt tgtggcagtg attcttaagc tttggtctcc cagaattttt ctagtctcgt
     1141 ttaccatctt catttcttcc tttgtataac cgttgggaag aaaaacatca atgtggggta
     1201 aacaattgcc tgctatcggg tgagggaatt tttgcggagc ttctccctta aggccgttct
     1261 caaggtcggc atatcccttc atacctgcac cggatacagc ctggtatgtt gaatacacta
     1321 ttcttttgat tttatacttg tcgtgcaacg gctttaatgc cacaacagcc tgtattgtag
     1381 agcagttggg gtttgcgatg attcccttgt tccatgaaat atcctgagga ttaacctccg
     1441 gtactacaag aggaaccgtt tcatccattc tccactgact gctgttgtca attacaacac
     1501 aaccctttga ggcagcaata ggtgcaaatt ctttacttgt gcctgctccg gcagagaaca
     1561 gagcaatatc aatgccccgg tcaaaggatg tttctgtaag ctcttctaca gtatattcct
     1621 tgccgttgaa agttattgct gtaccggcag agcggcttga agaaaaaaag tatatatttt
     1681 ctatgggaag atttctttct tccaatactt ttatcattgt tcttccaacc attccagtag
     1741 caccaacaac tgctaaatta atctttttca tatagataac ctccttgata taataacatt
     1801 ataaacattt ttctttagtt tgatagccgg gggattgtca tcataatgct gatgtgaatt
     1861 gatgtaaata ctaaaaaagc tggtatggat accagcagtt catttgcata tagtaatata
     1921 gttaatttaa gtttatgaac tgatagcgct ccatcaaata cttgatgaca gttatacagt
     1981 tgttagccgt ataaccagat tgcaccttta tgagcagata caaccttcgg cgatagaccc
     2041 tttcaacctt catcactaaa gctcatactt ttcagaagac ttactattga ataccgcacc
     2101 tctatcaaac aaattaatat ttagtttatt ttaaatcatt ttaacatctt aaatagtata
     2161 tgtcaaacaa tatttttttg ttgtttgtgt caagtaaaag ttggcaatga tctcttcatc
     2221 ctgttatttt attgg
//
LOCUS       DAOOJC010000407         2234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_38296_length_2234_cov_3.536485, whole genome shotgun sequence.
ACCESSION   DAOOJC010000407 DAOOJC010000000
VERSION     DAOOJC010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38296_length_2234_cov_3.536485"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLA01_09575"
     CDS             <1..639
                     /locus_tag="PLA01_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPD01581.1"
                     /translation="DRFSRERLSKLVDKINSLEPDIILLGGDFISKGSHYIKPCFEEL
                     KRLEAPLGKYGVLGNHDYYTSSEITRENMRKAGIAVLDNNARWIVYGGGRIKVGGVGD
                     YYHASQQIEPTIEDVREEDFVILLSHNPDYAEQIRNHKVDIVLSGHTHGGQVTFFGLW
                     APYIPSGYGQKYRYGMAETEYTKVYVTSGVGCTGYAVRFFARPEICVIELAN"
     gene            798..>1595
                     /locus_tag="PLA01_09580"
     CDS             798..>1595
                     /locus_tag="PLA01_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M4"
                     /protein_id="HPD01582.1"
                     /translation="MKRKIVLVIALAMIASLVFPSFVMAEGDPGLENAIKTVKKYFEI
                     PEELSIFSYSVDSRDGISYWNLNWYGKDYKGSNISATVDSTGDILYYSCYKYEEGQQE
                     YGRLPKISKAQAKSKAEEIIEKINPGILAQLKPVENNESVSLNSRLYSFRYERIYDGV
                     PMPSNGIIVYIDKQTGELCDYQKTWAKDLSFSSKDNILSLEEAQKAYIKNLGLRLTYN
                     AVSDMNGVKVYLAYSPVYNSAYYINAATGERFYFGALYGAGSIIMNEV"
     assembly_gap    1596..1605
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1606..>2234
                     /locus_tag="PLA01_09585"
     CDS             <1606..>2234
                     /locus_tag="PLA01_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M4"
                     /protein_id="HPD01583.1"
                     /translation="GVVLSPEEIAAIEEVSSLITQEQAEKIARNLKALKLDDTFELAR
                     AGLYKRYPENKGYNWDLYFSSIDEEGKINRSSNISIDAKTGEIVNFNTYYYDGEVTPK
                     FDKDDAKEAVEKLLNEIQPDKFKETELDEQQEDAIVYPEGEQPSSYAFSYTRKVNGVL
                     FPENGIYVQYDAITGNIISYRLTWYDVEFPSAEKVLPIEKIYETFFDQI"
BASE COUNT          724 a          358 c          540 g          602 t
ORIGIN      
        1 gatcgttttt caagagaaag gctttcaaag ctggtggata agattaattc cctggagccg
       61 gatattattc ttttgggtgg tgattttata agcaaaggaa gccactacat aaaaccgtgt
      121 tttgaagagc ttaagaggct tgaggcaccg ctggggaagt atggtgtttt gggaaaccat
      181 gactattaca caagtagcga gattacaaga gaaaacatga gaaaagctgg gatagccgtt
      241 cttgacaata atgcccgctg gattgtttac ggtggcggca ggataaaggt gggcggggtc
      301 ggagattatt atcatgcctc tcaacagata gaacctacta ttgaggatgt aagggaggaa
      361 gactttgtta ttttgctttc ccacaatccc gactatgctg aacagatccg aaaccataag
      421 gtagatatag tattgagcgg tcatactcac ggagggcagg taacgttttt cggcttatgg
      481 gctccgtaca ttccttccgg atacggccag aaatacagat atggcatggc tgagacggaa
      541 tacacaaagg tttacgttac aagcggtgtg ggctgtacgg gttatgctgt caggtttttc
      601 gcccgtccgg agatttgtgt gatagaattg gcaaattagc aatactacgg gtaaacaatg
      661 ttattcaaag aaaattgtgg aagcaatatt agtttaatgc atccagagta taatctttga
      721 aattttactg gaactttttt atgatttaat ccatctaata tgtataacga aaaatttaaa
      781 ctaaggagtg ggtgtaaatg aaaagaaaaa tagtgcttgt tatagcactg gctatgattg
      841 cgtcattggt tttccccagc tttgtgatgg cagaagggga tccggggctt gaaaacgcaa
      901 ttaaaacggt aaagaaatat tttgaaatcc cggaggagct tagcatattt tcctatagtg
      961 ttgactccag agacggtatc agttattgga atttaaattg gtatggaaag gattacaaag
     1021 gttccaatat ttcagctacc gtagacagca caggagatat actgtactat tcttgctata
     1081 agtatgagga gggacagcaa gaatacggaa ggcttcctaa aataagcaag gcacaggcaa
     1141 agagtaaggc agaagaaatt atagaaaaga ttaatccggg aatccttgca caattgaagc
     1201 cggttgaaaa taatgagagt gtatcactga atagcagact gtattctttt agatatgaaa
     1261 gaatatatga cggagtgcct atgccgtcaa acggaataat agtatacata gacaagcaaa
     1321 ccggcgagct ttgtgactat caaaagacat gggcaaagga tttgagcttt tcttcgaagg
     1381 ataatatatt gagtctggaa gaagcacaga aggcttatat taagaatctc ggactcagac
     1441 ttacatataa tgcggtatct gatatgaatg gcgtaaaagt atacctggca tattcgcctg
     1501 tttacaattc tgcttattat attaatgctg caacgggtga aaggttctat tttggagctc
     1561 tttacggtgc cggcagtata attatgaatg aagttnnnnn nnnnnatggg gtagtactta
     1621 gtccggaaga gatagcggca atagaagagg tttcatcact tataactcag gaacaggctg
     1681 aaaaaatcgc aaggaattta aaggctctga agctggatga cacctttgag ctcgcaagag
     1741 ccggacttta taaaagatat ccggaaaata aaggttataa ctgggacttg tatttttcaa
     1801 gcatcgacga ggaaggtaaa ataaaccgca gttcgaacat atccatcgat gcaaagacag
     1861 gagaaatagt gaacttcaat acgtattatt atgacggtga agtaactccc aagtttgaca
     1921 aggatgacgc aaaggaagct gtagaaaagc ttttgaacga gatacagccg gataaattca
     1981 aagagaccga gcttgatgaa caacaagagg acgcaattgt ttacccggag ggtgagcagc
     2041 ccagtagtta tgctttcagt tatacaagaa aggtaaacgg tgttttgttc cctgagaacg
     2101 gcatatatgt tcagtatgat gccatcaccg gtaatattat cagctatagg ctaacctggt
     2161 atgatgttga gtttccttcg gcagaaaagg ttctgcctat agaaaaaatc tatgagactt
     2221 tcttcgatca aatc
//
LOCUS       DAOOJC010000408         2221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_38672_length_2221_cov_2.767775, whole genome shotgun sequence.
ACCESSION   DAOOJC010000408 DAOOJC010000000
VERSION     DAOOJC010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38672_length_2221_cov_2.767775"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PLA01_09590"
     CDS             complement(<1..454)
                     /locus_tag="PLA01_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPD01584.1"
                     /translation="MVERISVLGSTGSIGVQTLDVARNLNIKVDGLAANKNIELLEKQ
                     ARKFKPEIVAVMDENQAAVLRSRLEDTGIEVAGGVEGLKKVACVESAQTVVTAIVGIA
                     GLVPTMEAIKSGKNIALANKETLVTAGSIVMAEAKKQKVEILPVDSEHS"
     gene            complement(533..1375)
                     /locus_tag="PLA01_09595"
     CDS             complement(533..1375)
                     /locus_tag="PLA01_09595"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPD01585.1"
                     /translation="MLKTRVASASVAILILIAVVCLGKTAIGVSVFFVSVIAMYEYFK
                     ALENATYRPVKPIGYISCLYILLICFEYVSFGSLKLFQNMLSSKFLSMVIFLMLLALL
                     FCIVFLHQKYNIVDVAVTFFGVFYITFLFSFIVLIRNLENGFFLVWLVFIGAFSTDTF
                     AYFSGRFFGKRKLMPAISPKKTVEGAVGGMLGCTVVTVIYGVYLLSSGNVPDISLYHY
                     IVLGILCGTISQFGDWAASAIKRFVKVKDFGNIMPGHGGAIDRFDSILFTAPVIYFYV
                     SFIV"
     gene            complement(1378..2130)
                     /locus_tag="PLA01_09600"
     CDS             complement(1378..2130)
                     /locus_tag="PLA01_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPD01586.1"
                     /translation="MIFFRNIFKKSKKTEIDYNNLPQHIAIIMDGNGRWAKKRSLPRS
                     MGHMEGAKTLKRISTFCGEIGIKYLTVYAFSTENWKRPKSEVDALMSLLLDYLKNAET
                     HIGGKNVRINTIGDVSVFNDEIKKEIDRVTNITGKNTGLVLNIALNYGSRDEIVHAVK
                     EIAMELERGKIKTGDISEEMLNDKLYTSGVPDPDLLIRPGGEKRLSNFLLWQSAYTEF
                     WYTDTLWPDFKEEHIIEAILDYQNRNRRFGGI"
BASE COUNT          695 a          536 c          322 g          668 t
ORIGIN      
        1 ccgaatgctc actgtctacc ggaagaatct ccactttctg ttttttagcc tctgccataa
       61 caattgaacc tgcggtcaca agagtctcct tgttggcaag ggctatattt ttcccgcttt
      121 ttatggcttc cattgtaggt acaagcccgg caatcccaac aatagctgta acaacagtct
      181 gggcactttc aacacaagca acctttttta atccttcaac tccacccgcc acttcaatac
      241 cggtgtcttc aagtcttgac cttaaaaccg ctgcctgatt ttcatccatt actgcgacaa
      301 tctcgggttt aaacttcctc gcctgctttt caagcagctc aatattctta tttgccgcca
      361 atccgtcaac ctttatatta agatttctag ccacatccag ggtttgcaca ccaattgaac
      421 ccgtggaccc caaaaccgat attctctcca ccatgtatat atctccttca attattcatt
      481 atgaagcatc ccaacagaac aaaagcaatt cactcttaac tgtttgaggc cgttacacaa
      541 taaagctgac atagaaataa attacaggag ccgtaaacag tatactgtca aacctgtcaa
      601 tagctccgcc atgccccggc ataatatttc caaagtcttt aaccttgaca aatctcttta
      661 tggcagatgc cgcccagtct ccaaactgtg atattgtacc gcataaaatc cccaacacta
      721 tgtagtggta cagggaaata tcaggaacat tgccgcttga taaaagatac actccatata
      781 ttaccgttac aactgtacag ccaagcatcc cgcctactgc tccttccaca gtttttttcg
      841 ggcttatcgc cggcataagc tttcgtttgc cgaaaaagcg tcctgaaaag tatgcaaagg
      901 tatctgtgga aaaagccccg ataaaaacaa gccatactaa gaaaaatccg ttttccagat
      961 tccttataag tactataaac gaaaataaaa aagttatata aaaaacaccg aagaatgtaa
     1021 cagcaacatc cacgatatta tatttctgat gtaaaaatac tatacagaat aataatgcca
     1081 acaacattaa aaaaattacc attgacagaa atttagacga taacatattt tgaaacaatt
     1141 ttaaagatcc aaaggaaacg tattcgaaac aaatcaacag tatataaaga cacgaaatat
     1201 agcctatagg ctttactggc ctatacgtag cattttcaag tgctttaaag tactcataca
     1261 tggcaattac agaaacaaaa aaaaccgata ctccaatagc agttttacct aaacatacaa
     1321 ctgcaatcag tataagaatt gccacagaag cacttgccac tctggtttta agcaatttta
     1381 tatccctcca aatcgtctgt ttctgttctg ataatccaaa atggcctcaa ttatatgctc
     1441 ctccttaaaa tcaggccata gtgtatcagt ataccagaac tcggtataag ccgactgcca
     1501 taacaagaaa ttgctcagcc tcttttctcc tccgggtctg attaataaat cgggatccgg
     1561 cactcccgaa gtataaagct tgtcatttaa catctcctca ctgatgtccc cggtttttat
     1621 ttttccccgt tccaattcca ttgcaatttc ttttacagca tgtacaattt catctctgct
     1681 gccgtagttg agggctatgt ttaacacaag acctgtattc ttgccggtaa tattagttac
     1741 acgatcaatc tctttcttta tttcatcgtt gaaaacactc acatcaccaa tggtatttat
     1801 ccgtacattt ttacccccaa tgtgggtctc agcatttttc aaataatcca gaagaaggga
     1861 catcaaagca tcaacctcac tcttgggtct tttccagttt tcggtcgaaa aagcataaac
     1921 agtaaggtat tttattccaa tctctccaca gaaggttgat attctcttca aggtttttgc
     1981 accttccata tgccccattg aacgcggtaa tgacctcttt ttagcccatc tcccgtttcc
     2041 gtccatgatt atagcaatgt gctgcggcaa attattgtaa tctatctccg tttttttgct
     2101 ttttttaaat atattcctga aaaagatcat tataacctcc aatactgctg acaaaccctc
     2161 gaactgcaat tattattata ttatatatat aagctttgtt aaaatcaata aataccataa
     2221 c
//
LOCUS       DAOOJC010000409         2219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_38717_length_2219_cov_4.020795, whole genome shotgun sequence.
ACCESSION   DAOOJC010000409 DAOOJC010000000
VERSION     DAOOJC010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38717_length_2219_cov_4.020795"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /gene="dinB"
                     /locus_tag="PLA01_09605"
     CDS             complement(<1..927)
                     /gene="dinB"
                     /locus_tag="PLA01_09605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPD01587.1"
                     /translation="MNRTILHCDLNNFYASVECLYNPELRDQPVAVCGSAEDRHGIVL
                     AKNYIAKSYGVRTGEATWEAKSKCPGLVTVRPNYSLYLKFSRYARQIYEYYTDRIESF
                     GIDECWLDVSESSLLFGDGIKIANEIRKRVKSEMGVTVSIGVSYNKIFAKLGSDMKKP
                     DAVTVITEDDFKEKVWRLPVSELLYVGRSTRAKLNNLAIFTIGELANCSSKFLVKQFG
                     KWGNILWTFANGYDISPVTKLGYESVIKGIGNSITTSRDLTKNEDVKILIYVLSESVG
                     ERLRRHNFKGRTVQISIRDTALKSIDRQGKLDR"
     gene            complement(1021..1644)
                     /locus_tag="PLA01_09610"
     CDS             complement(1021..1644)
                     /locus_tag="PLA01_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012344223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HPD01588.1"
                     /translation="MKNKIDVYIKVEGHGKIPEYASENAAGCDLYATEDMAIRPGETK
                     IMPLDFIVAFPPEIEAQIRPRSGLSLKTDLRLPNGPGTIDSDYRDTVGVLIQNTYNIA
                     NLPYQIAQNPEILLELKNNYREIELREYLSLRDGTDIENFDGLDVLNRKMYVDKKGNP
                     FGTIYISRGDRIAQMIFCEYKAANFISHPNPQEIGNNRGGGFGHTGV"
     gene            complement(1731..>2219)
                     /locus_tag="PLA01_09615"
     CDS             complement(1731..>2219)
                     /locus_tag="PLA01_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPD01589.1"
                     /translation="TLSPELNLNQIKEMEKFPGLTAEVIVYGRIPLMISEYCPVGSIR
                     GGMCRNKKCSRECIGKDFGLRDRKNMEFPILCDRIDCRSMIFNSNVLLLADDIDKIKA
                     SGADMIRLNFTDEEPDEVMDIIDMHKALLIDDSKYRKVYNGMIEKIKAGGFTKGHYFK
                     GV"
BASE COUNT          551 a          561 c          388 g          719 t
ORIGIN      
        1 acggtccagc ttgccctgcc tgtctatgct tttaagtgcc gtgtccctta tgcttatctg
       61 caccgttctt cctttaaagt tatgccttct gagtctttcc cccacgctct cggaaagtac
      121 atatattaaa atcttaacat cttcattttt tgttaaatcc ctggaagtag tgatggaatt
      181 gcctatcccc tttatgaccg attcataacc gagctttgta acagggctga tatcgtagcc
      241 gtttgcaaaa gtccacagaa tgtttcccca cttcccaaac tgctttacaa gaaatttgga
      301 agaacagttt gccagctccc caatggtaaa gatggcaaga ttattgagtt tggctcttgt
      361 tgaccgtcct acatacaaaa gttcgcttac aggaagtctc catacctttt ctttaaaatc
      421 atcctcggtg ataaccgtaa ccgcatcggg ctttttcata tcagacccaa gctttgcaaa
      481 tattttgtta tagcttaccc ctatggaaac cgtcacgccc atttcgcttt ttaccctttt
      541 ccttatttcg ttggctatct taatcccgtc tccaaacaaa agagagctct cgctgacatc
      601 cagccaacac tcatctatac caaaggactc tatcctgtcg gtataatatt cataaatctg
      661 gcgtgcatac cttgaaaact tgagatacaa gctataattc ggccttaccg ttacaagccc
      721 gggacacttg cttttggcct cccatgtagc ctcaccggtt ctgacaccgt agctctttgc
      781 tatgtagttt ttggcaagaa ctataccgtg cctgtcttct gcagagccgc atacagcgac
      841 aggttgatca cgaagctcag gattatacag gcattctaca ctggcataga aattattaag
      901 gtcacaatgc aaaattgttc tattcataaa cccaccctct tgcatcgaac atgtgttcgt
      961 atttatatta tacggcatga gggatgtgtt tgtaaagtcc gatttttttg catgcaccca
     1021 ttaaacccct gtatgtccga agcctccccc tctgttattt ccgatttcct ggggattggg
     1081 atgggaaata aaattagctg ctttgtactc acaaaaaatc atttgtgcaa ttctatcccc
     1141 ccgggatatg taaatagtac caaacgggtt tcctttttta tccacataca tttttcggtt
     1201 taagacatcc agcccgtcaa aattttcgat atccgtgccg tctctcaagg acaagtactc
     1261 cctcagctca atttccctgt aattattctt caactctaaa aggatttcag ggttttgtgc
     1321 aatttgatac ggcaagtttg caatgttata ggtgttctgg attagaaccc ccacggtatc
     1381 cctgtaatca ctgtcaatcg tgcccggtcc gttgggaaga cgcaggtcgg tttttagcga
     1441 taacccgctt ctgggcctga tttgtgcttc tatttccggc ggaaacgcaa caataaaatc
     1501 aaggggcatt attttggttt ctccgggccg tattgccatg tcctctgttg cgtacaagtc
     1561 gcaccctgcc gcattctccg atgcatactc cggtatcttg ccatgacctt ccacctttat
     1621 atatacgtcg attttatttt tcatattgaa cctccccgtt tttatcaaat atttattcgc
     1681 ccaaaagagc tcaagaaccc tggcagcctg actgaatatc cctttaagac ctacacgcct
     1741 ttaaaatagt ggcctttggt aaatcctcct gcctttattt tttcaatcat cccgttataa
     1801 acctttcggt acttagagtc atctattaaa agggctttgt gcatgtctat tatatccatg
     1861 acctcatcag gctcttcatc cgtaaaatta agccttatca tatctgcccc ggatgctttt
     1921 attttatcaa tatcgtccgc aagaagtaat acattggaat taaaaatcat gcttctgcaa
     1981 tcaatcctat cgcacagtat gggaaattcc atgttctttc tgtccctgag cccaaaatcc
     2041 ttacctatac attcccggct gcattttttg tttctgcaca ttcctcccct gatacttccc
     2101 acaggacagt actcgcttat cataaggggt attcttccgt acacgatcac ttcggctgtg
     2161 agtccgggaa atttctccat ttcctttatc tgattcaaat tgagctccgg tgataaagt
//
LOCUS       DAOOJC010000410         2215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_38807_length_2215_cov_3.770370, whole genome shotgun sequence.
ACCESSION   DAOOJC010000410 DAOOJC010000000
VERSION     DAOOJC010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38807_length_2215_cov_3.770370"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..764
                     /locus_tag="PLA01_09620"
     CDS             114..764
                     /locus_tag="PLA01_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPD01590.1"
                     /translation="MDKIRIAIADDHAMVRQGLKQILELEDDIVVVAQAANGEDAVKV
                     AKEDHPDVILMDINMPGINGLQAIKEIKEEEIPCGIIVLTIHEDREYLFKTLQLGAQG
                     YVLKDADPAVLVEAIRSVHRGQPYIQANMTKELVNEFNRVMTHDKGKGEENSLTAREL
                     EVLELIAQGLINKEIAKRLYISEKTVKNHVSNIFRKINVSDRTQAAIYAFKHNITN"
     gene            1387..1581
                     /locus_tag="PLA01_09625"
     CDS             1387..1581
                     /locus_tag="PLA01_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HPD01591.1"
                     /translation="MLKMYVVYLRALLGNKKGQGMVEYGLIISLIAVACITALLLLGP
                     KIAELFTSVSDTLTPASGTP"
     gene            1644..1838
                     /locus_tag="PLA01_09630"
     CDS             1644..1838
                     /locus_tag="PLA01_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HPD01592.1"
                     /translation="MLKMCEVYLRMLLGNKKGQGMVEYGLIISLIAVACIAALLVLGP
                     KIAELFSSVSDTITPASGTP"
     gene            2086..>2215
                     /locus_tag="PLA01_09635"
     CDS             2086..>2215
                     /locus_tag="PLA01_09635"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HPD01593.1"
                     /translation="MVETALILPIVVLLLTGIIDFGILFNNYLVITNASREAARNAA"
BASE COUNT          734 a          325 c          507 g          649 t
ORIGIN      
        1 catgaaagaa agagtggagc tcttaaacgg agaatttgaa atagattctg ttgttggtaa
       61 agggacgaga ctaaatataa cagtaccttt agttccggaa gagggggtct aaaatggata
      121 agatacgtat tgcaattgca gacgaccatg ctatggtcag acaaggtctt aaacagattt
      181 tagaacttga ggatgatata gttgtggtgg cacaggcagc aaacggagag gatgcggtga
      241 aggtcgcaaa ggaagatcat ccggatgtga ttcttatgga cattaatatg ccgggaataa
      301 atggcttgca ggcaataaag gaaataaagg aggaagaaat tccttgcggt ataattgtcc
      361 ttactattca cgaagacagg gagtatttgt ttaagacttt gcagttgggg gctcagggat
      421 atgtgcttaa agatgcggac ccggctgtac tggttgaagc cattagaagt gttcatcggg
      481 gacagcctta catacaagcg aatatgacaa aagaactcgt taatgagttc aatagggtaa
      541 tgacccatga taagggtaaa ggcgaagaaa acagcctgac agcgagagaa ttggaggttc
      601 ttgagcttat agcccaggga ttgattaata aggagatagc aaaacgcctt tacataagtg
      661 aaaagacagt taaaaaccat gtttctaata tttttagaaa gattaatgta tctgacagga
      721 cacaagctgc aatatatgcc ttcaaacaca atattacaaa ctagaataat gagagtttga
      781 ataaaaaaag gacattcgat ggaatgttct tttttttatg atactttatt ttatgggata
      841 tttttcttca ctcttaatac tgtaaaacat gcatagagtc cggataatag gacgtgttta
      901 ataggacttc ctacaattct tctaggtatt tttatttatg ttaatcgggg cactcgatgg
      961 atagcatatt aaaccgtgac aatttataat aaaagtgtaa ggtgattaac cgaccttaca
     1021 ggcaaggggg ataacataaa ccatgaaact gtttggagta ttatgtaaag tttttaaaaa
     1081 acaaaagggt caaggacttg tggaatacgc atttataatt atcttcattg ctatggcatt
     1141 atttgttgta cttggcttga tgggctcatc cttagtaagt ttctttaact acttcatgtc
     1201 acttttctaa ataaatattg aacgaagact ctaaactgaa ttattagaac cgttcataag
     1261 tattatgcat atgatttcgc aataattaaa acaaaggaaa ataaaagatt atgtgcggaa
     1321 cgtgtgtata tctattcaat aaattcgttg cggaggaaat aaaaaataag aaaggtggaa
     1381 acaagaatgc taaaaatgta tgtagtttat ctcagagcgt tgttaggaaa caaaaaagga
     1441 cagggaatgg ttgagtacgg actcatcata tcattgatag cagttgcatg tataacagcg
     1501 ttattgttgc tgggtccgaa gatagctgag ctgtttacat cggtatccga taccctgact
     1561 ccggcatcag gtactccgta atccaagtta ttggcaatta ttaattaaat aaactattat
     1621 aagttgaaag gtggaaaata attatgttaa aaatgtgtga ggtttatctc agaatgttgt
     1681 taggaaacaa aaaaggacag ggaatggttg agtacggact catcatatca ttgatagcag
     1741 tagcatgtat agctgcgtta ttggtgctcg gaccaaagat agctgaactt ttttcatcgg
     1801 tatccgatac aataactccg gcttcaggta ctccgtaatt tgtgtataac aaactaccct
     1861 atctgatttc agatagggtt attttaaagc taagtgtcag gtaaagcagg ttaagagctt
     1921 aagggggctt ttttgccccc aggcttttgt tagttggatt tcacggagtg aaatgactga
     1981 aaagcgaaag ggctcgatgc ccttgagctt ttactttcaa tgggggaaag gtgtggtttt
     2041 atgtatttga aacggaagct tttgggcagc cagaagggtc agtccatggt agaaaccgct
     2101 ttgatacttc cgatagtagt cttattattg acaggtataa tcgatttcgg tatactgttc
     2161 aacaattatc tggtaattac caatgcctcc agagaagctg caagaaatgc cgcag
//
LOCUS       DAOOJC010000411         2212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_38880_length_2212_cov_3.702828, whole genome shotgun sequence.
ACCESSION   DAOOJC010000411 DAOOJC010000000
VERSION     DAOOJC010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38880_length_2212_cov_3.702828"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLA01_09640"
     CDS             <1..505
                     /locus_tag="PLA01_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPD01594.1"
                     /translation="NKPVGYISSAKDQFGRPTVLDLVTDLKERVFPVGRLDYDTSGLI
                     LLTNDGDFTYRLTHPKHEIEKVYEAVLDGVPKKEDIERFEKGLKIDDYITSPAKFEII
                     KCDSKTTTAKITIHEGKNRQVRKMCEKIGCPVIKLKRISIGKVVLGNLKEGEWRNLTP
                     KEIESFS"
     gene            complement(520..1059)
                     /locus_tag="PLA01_09645"
     CDS             complement(520..1059)
                     /locus_tag="PLA01_09645"
                     /inference="COORDINATES: protein motif:HMM:NF036614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5362 domain-containing protein"
                     /protein_id="HPD01595.1"
                     /translation="MDDEIKLPEDTLNTPEENLQYNEEKMDVSQENRHNLQENKNVSI
                     EEQYVPQQQKQFTQENQHVQEQKLQALVQYKRISGWATFMGVFAVVTGLVLCLGIVTA
                     IIGVPLIIAGLRLTGAVDSMKEFTRTNDVNKLSDMVDKINTYFRIYGILTLITLLVGF
                     ISLIALITYSIISGNTVLF"
     gene            1255..2082
                     /gene="speE"
                     /locus_tag="PLA01_09650"
     CDS             1255..2082
                     /gene="speE"
                     /locus_tag="PLA01_09650"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HPD01596.1"
                     /translation="MELWYTEFQTPAVGITCKTKKTYHTEKTQYQDLALIETEQFGRM
                     LVLDGTVQTTIEDEFVYHEMITHVPLFTHKNPKKVLVIGGGDGGTIREIIKHPSVEKA
                     VLCEIDEKVIEVSKKYLPEISCALGNDKVVVKVGDGIKYVHEHKNEFDVIIVDSTDPV
                     GPAEGLFAVDFYKAISEALKEDGIFVAQTESPFFHKDLIKKVYKDVKSIFPIARLYTC
                     AIPTYPSGYWSFTMGSKKYDPLETDINTITDMNTKYYCKEIHKSVFALPKFVADLVK"
BASE COUNT          734 a          391 c          461 g          626 t
ORIGIN      
        1 gaacaaaccc gtggggtata tcagttcggc aaaggatcag tttggaaggc ctacagtgct
       61 ggatttagtc actgatttaa aagaaagggt atttcctgtg ggaaggcttg attatgatac
      121 atccggctta attcttttaa ccaatgacgg tgacttcaca tacaggctta cacatccgaa
      181 gcacgagata gaaaaggttt atgaagcggt gctggacggg gttcccaaaa aagaggatat
      241 agaaagattt gagaagggac tcaaaatcga tgattacatt acatctccgg caaagtttga
      301 gattataaaa tgtgacagca aaacaactac tgcaaaaata acaatacacg agggcaaaaa
      361 caggcaggta agaaaaatgt gcgaaaaaat cggctgcccg gtaataaagc taaaaaggat
      421 ttcaattgga aaagttgttc ttggaaacct caaagaaggg gagtggagga acctcactcc
      481 aaaagagata gaatccttca gctaaaatta tgttacaaat caaaacaaaa ctgtatttcc
      541 ggatattata ctgtaagtga tcagggctat caatgatata aagccgacta acagtgtaat
      601 gagagtcagg attccatata ttctgaaata cgtgtttatc ttgtcaacca tatccgaaag
      661 cttgtttaca tcattggtac gagtgaattc cttcatgctg tccaccgcac cggtaagtct
      721 cagtcccgca ataataagag gtacaccgat aatggcggta acaattccaa ggcagagcac
      781 cagccctgtt acaacagcaa aaacccccat aaaggttgcc catcccgaaa tccttttgta
      841 ctgaaccaaa gcctgtaatt tttgctcttg aacatgttgg ttttcctgag taaactgctt
      901 ttgttgctgc ggcacatatt gctcttctat tgaaacattc ttattctcct gtaaattatg
      961 ccggttttcc tgtgatacat ccattttttc ttcattatac tgcaggtttt cttcaggagt
     1021 gtttaaagta tcttccggta atttaatttc atcatccatt aaatatcaac ctccgtctgg
     1081 ttttggtcgg ccattttagt ttgactctat aaaccagatt ttattatatc ataatatgtg
     1141 aaaaaattgt tttgacaaaa cagtatttcc tatttaaaat attaatggca aatgaaaaaa
     1201 atggacagaa taatattaat tctgtttctt actattatga caggagtgac tgaaatggag
     1261 ctttggtata ccgaattcca gacccctgca gtggggataa catgcaagac aaagaagaca
     1321 tatcatactg aaaaaactca gtatcaggat ctggcactta tagaaaccga acagttcgga
     1381 agaatgctgg ttttagacgg cacagttcag actacgattg aggacgagtt tgtttatcat
     1441 gagatgataa cccacgttcc cctgttcact cacaaaaatc cgaaaaaagt attggttatc
     1501 ggtggcggag atggaggaac aataagagaa ataattaaac acccatcggt ggaaaaggca
     1561 gttttatgcg aaattgacga gaaggtaata gaggtttcca aaaagtatct tcccgaaatc
     1621 agctgtgctt tgggaaatga taaggtcgta gtaaaagtgg gtgatggaat aaaatatgtg
     1681 catgagcata aaaatgagtt tgatgttata atcgttgatt caacagaccc tgtaggtcct
     1741 gctgaaggtt tgtttgctgt tgacttttac aaggctatta gtgaggcatt aaaggaagac
     1801 ggtattttcg ttgcacagac tgagtcaccc tttttccata aagatttgat aaaaaaggtc
     1861 tataaggatg taaaatccat attccccata gcaaggctct atacctgtgc aattcctaca
     1921 tatcccagcg gatattggag ttttacaatg gggtctaaaa agtacgatcc tttagaaacc
     1981 gacataaaca caataacgga catgaacaca aaatattatt gtaaagagat tcataaatcg
     2041 gtttttgccc ttcctaagtt tgttgctgat ctggttaaat aggataaagg gatcgaaata
     2101 ggtataaatg gaattcaaca tgataaaaac catcggctgc taagccggtg gttttaggtt
     2161 gtgtgccagg catggcacta atctagctag tgaaagtcta gccacgggta tt
//
LOCUS       DAOOJC010000412         2211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_38897_length_2211_cov_4.493970, whole genome shotgun sequence.
ACCESSION   DAOOJC010000412 DAOOJC010000000
VERSION     DAOOJC010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38897_length_2211_cov_4.493970"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..1333)
                     /locus_tag="PLA01_09655"
     CDS             complement(212..1333)
                     /locus_tag="PLA01_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPD01597.1"
                     /translation="MGIDLNKMPRLGFGMMRLPEKDGEIDIPRVCNMVDVYMEKGFNY
                     FDTAYVYHGGKSEVAVRETVVKRYPRDSFYVASKLPAWEIKKAEDCDRIFNEQLERAG
                     VDYFDFYLLHSIEEGNNYDTYIKYNCFDWAMEKKKKGQIKHFGFSFHGSPTLLKKILS
                     EHPKVEFVQIQLNYADWNSPIVHSGKLYEILREHNMPIIVMEPVKGGMLASTTPELEA
                     MMKKLQPDKSIASWAFRFVGSLDGIMTILSGMSNEEQLNDNIKTFSNFEPLSDKEREV
                     LDEVTKAMAKSSVIQCTSCRYCCDGCPQKISIPDVISALNTLRLYGEDARPHFFYGNL
                     VDKSGRAGDCISCRQCEDVCPQHLPIIDIMQEASRIFDK"
     gene            complement(1372..>2211)
                     /locus_tag="PLA01_09660"
     CDS             complement(1372..>2211)
                     /locus_tag="PLA01_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HPD01598.1"
                     /translation="EYIVSDAQIRKQVIKSIVDQINSTDVGDGSFVSFNGVVIDFEDM
                     RGEVLKQSFNLFLSELKEELEKNGKLLYVAVHPAGPAGSSYYDGYDFKTIGEIADKVI
                     LMAHDYYAKQLDDVEMKIGYTLTPLTPINEIYYALRAITDKDTGIQNLDKIWLQLSFD
                     SVQWKLKDGKVINKYPYNPGYDAIYKRLVMDGVTVKYSDSFQNPCALYYDDKDGTDNV
                     LWYEDSRSIQAKIDLAKMFGIRGISLWRLGNIPDYEDQGEKEIYLNVWRQIMQNTEKT
                     NDK"
BASE COUNT          636 a          492 c          355 g          728 t
ORIGIN      
        1 taagaatata caggatttac ttttggaatt atattacttc atgcgatagc cctgagtgca
       61 gtgggtgcat taaaaattgc tgaagtattt tttcccaatc cttatcgcag atatttattg
      121 ataaaggata tttacataat ataaaaaatc ctatatccca attattccgc aaacaggtat
      181 tttcttcaca tttattcaat gcacccacaa actatttatc aaaaattctg gaagcttcct
      241 gcattatatc gataatcggc aaatgttgtg gacatacatc ctcacattgc cggcaagaaa
      301 tgcaatcacc ggctctgccg cttttgtcca ccaaattacc gtagaaaaag tgaggtcttg
      361 catcttctcc atacagacgc aaagtattta aagcacttat tacatccgga atactgattt
      421 tttggggaca tccatcgcag cagtatctgc atgaagtaca ttggataaca gatgattttg
      481 ccatagcctt cgtcacttca tcaagaactt ccctctcttt atcgcttagg ggctcaaagt
      541 tagaaaaggt ctttatatta tcgttaagct gttcttcatt tgacatgccg ctcagtatcg
      601 tcataatacc atccaaagag cccacaaaac gaaatgccca agaagcaata cttttgtcgg
      661 gttgcagctt tttcatcatg gcttcaagct ccggagttgt acttgcaagc atacctccct
      721 tgacaggctc cattacaatg atgggcatat tatgttccct gagtatttca tacagcttgc
      781 cggaatgaac aatagggctg ttccagtctg cataatttag ctgaatctgt acaaattcca
      841 ctttcggatg ctctgataga atcttcttta aaagtgttgg agagccatga aaagaaaatc
      901 caaaatgctt tatctgcccc ttctttttct tttccattgc ccaatcaaag cagttatatt
      961 taatatatgt gtcataattg tttccctctt ctatgctgtg aagcaggtag aaatcaaaat
     1021 agtctacccc tgcacgttca agctgctcgt taaatatacg gtcacaatcc tcagcctttt
     1081 taatttccca tgccggaagc tttgatgcaa cataaaagga atctcttgga taacgcttaa
     1141 caacagtttc ccttactgca acttcagatt ttcctccatg gtacacatag gcggtatcaa
     1201 agtagttgaa tcccttttcc atatagacat ctaccatgtt acataccctc ggtatgtcta
     1261 tttcaccgtc tttttccgga agacgcatca ttccgaatcc cagccttggc atcttgttta
     1321 agtcaatacc cattagattc ccctccatat taaatcgaaa ttaacgtaat gttatttatc
     1381 attagttttt tcggtgtttt gcataatttg ccgccataca ttaagatata tttctttttc
     1441 accctgatct tcataatcgg gtatgtttcc caatctccaa agagaaattc cccgtattcc
     1501 gaacattttc gccaggtcta tttttgcctg tatgcttcgt gaatcctcat accacaaaac
     1561 attatcggtg ccgtctttgt catcgtaata taaagcacaa ggattttgga atgaatcgga
     1621 gtattttacg gtaaccccgt ccataacaag ccttttatat atagcatcat agcccggatt
     1681 ataagggtac ttgtttataa ccttaccgtc ttttagcttc cattgcaccg aatcaaagga
     1741 caactgcagc catattttat ccaagttctg tattcctgtg tctttgtcgg taatagccct
     1801 taatgcatag taaatctcat ttataggggt taatggagta agggtataac cgattttcat
     1861 ttctacatcg tccaattgtt tcgcataata gtcatgggcc atcaatataa ctttatctgc
     1921 aatttctcca atcgttttaa aatcataacc gtcataataa gatgatcccg ctggtcctgc
     1981 cggatggaca gcaacataca acagcttgcc gttcttttca agttcttcct ttaattcaga
     2041 gaggaataag ttgaaagact gttttaatac ttcgcccctc atatcttcaa aatcaataac
     2101 aacaccattg aaggatacaa aactgccgtc cccaacatca gttgaattta tttgatccac
     2161 aatagattta atgacctgtt ttcttatttg ggcatcggaa actatgtatt c
//
LOCUS       DAOOJC010000413         2201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_39155_length_2201_cov_3.623952, whole genome shotgun sequence.
ACCESSION   DAOOJC010000413 DAOOJC010000000
VERSION     DAOOJC010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39155_length_2201_cov_3.623952"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..671
                     /gene="sigE"
                     /locus_tag="PLA01_09665"
     CDS             6..671
                     /gene="sigE"
                     /locus_tag="PLA01_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HPD01599.1"
                     /translation="MYGKSPIYYIGGSEALPPPLSNDEESYLLNKLEKSDYGVKSILI
                     ERNLRLVVYIARKFENTGVGVEDLISIGTIGLIKAINTFNPSKNIKLATYASRCIENE
                     ILMYLRRNNRMKTEISIDEPLNIDWDGNELLLSDILGTENDLIHRSIEDEVDRELLGI
                     AMKKLSVREKKIMELRFGLTNGGIEKTQKEVADMLGISQSYISRLEKKIISRLKKEIS
                     RMT"
     gene            830..1603
                     /gene="sigG"
                     /locus_tag="PLA01_09670"
     CDS             830..1603
                     /gene="sigG"
                     /locus_tag="PLA01_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HPD01600.1"
                     /translation="MLINKVEICGVNTSKLPVLSNEQKKVLFERMHNGDTSAREEFIR
                     GNLRLVLSVIQRFNNRGEYVDDLFQVGCIGLIKAIDNFDVSQNVKFSTYAVPMIIGEI
                     RRYLRDNNSIRVSRSLRDIAYKALQAKERLTNKNSKEPTVDEIAKELGIPKEDVVFAL
                     DAIQDPISLFESVYHDGGDAIYVMDQVKDDKNTDENWLEGISLNEAMRKLNDREKLIL
                     NLRFFDGRTQMEVAEEIGISQAQVSRLEKTALMHMRKYI"
     gene            1718..1978
                     /locus_tag="PLA01_09675"
     CDS             1718..1978
                     /locus_tag="PLA01_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HPD01601.1"
                     /translation="MNRTSDFREKEVINIKDGRRLGFVCDVEINLESGKIEAIVIPGD
                     RRWFGLVGKDDEYVIPWDRIKKIGEDIILVELDDRYIRKHFE"
     gene            2127..>2201
                     /locus_tag="PLA01_09680"
     CDS             2127..>2201
                     /locus_tag="PLA01_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPD01602.1"
                     /translation="MKCPYCGFIEDKVIDSRPTDEGSAI"
BASE COUNT          750 a          311 c          530 g          610 t
ORIGIN      
        1 ttaaaattta cggcaagtcc cccatttact atattggggg aagtgaagca ttgccgccgc
       61 cgttgagcaa cgatgaggag agctaccttt tgaacaagct ggagaaaagc gattatgggg
      121 ttaagtcaat tttgatagaa agaaatttaa ggctggtggt atacattgca agaaaatttg
      181 aaaatacggg agtgggagta gaggacctaa tttcaatcgg caccatcgga ctcattaagg
      241 ctataaacac atttaacccg agtaaaaaca ttaagcttgc cacgtacgca tcaaggtgca
      301 ttgaaaatga aatattgatg tacctcagaa gaaataaccg gatgaagact gagatttcga
      361 tcgatgagcc gcttaacatt gattgggacg gtaatgaatt gctgctttcg gatattctgg
      421 ggacagagaa tgatctgatt cacagaagta tagaggatga agtagacagg gagctccttg
      481 gaatagcaat gaaaaaactg tcggtgaggg aaaaaaagat aatggagctt cgctttggcc
      541 ttacaaacgg aggaattgag aaaacacaga aggaagtagc cgatatgctt ggcatttcac
      601 agtcgtacat atcaaggctt gaaaaaaaga ttataagcag gttgaaaaag gagatcagcc
      661 ggatgacctg attaattgaa gtaccataaa aaaccagttc aagtattttc aattactaca
      721 gggtttgtca aggcttttat gcaaaatttt gtggtgttat ttacagtata aaaataacct
      781 ccgtggcaat aatgatactg acttaaattt attgccggga ggttttttta tgcttatcaa
      841 taaagttgaa atttgtggtg taaacacatc gaaattgcca gtacttagca atgaacaaaa
      901 aaaggttctc tttgaaagaa tgcataatgg agatacctcg gcaagagagg aattcataag
      961 gggaaatcta agacttgtat tgagtgttat tcaaagattc aacaataggg gagaatatgt
     1021 tgacgacctt tttcaggtag ggtgtattgg acttataaag gcaattgaca attttgacgt
     1081 atcccaaaat gttaagtttt caacttatgc tgttcctatg attatagggg agattagaag
     1141 atatcttcgt gacaacaact ccatccgggt aagcagatca cttagggata ttgcatacaa
     1201 agcccttcaa gcaaaagaaa ggctgaccaa taagaactcg aaagaaccga cggtggatga
     1261 aatagctaaa gaactcggga ttccaaagga agatgttgtc tttgcccttg atgccataca
     1321 ggaccctatt tcgctgtttg agtccgtgta tcatgatggg ggagatgcaa tttatgtaat
     1381 ggatcaggtt aaggacgata agaatacgga tgaaaactgg ctggagggta tttcacttaa
     1441 tgaagctatg agaaagttaa acgacaggga gaagcttatt ctaaacttac gtttctttga
     1501 cggcaggact caaatggagg ttgctgaaga aataggaata tctcaggcac aggtttcgag
     1561 gctggaaaag actgcactga tgcatatgag aaaatatata taacttagaa tatgccaaaa
     1621 aagagtcaaa atattttggc tctttttttc aaatcttttt attaactctc atatattaag
     1681 ggtgagggac tttgtcagtg ttaaggggga gtgggatatg aacaggacat cggattttag
     1741 agaaaaggaa gtaatcaata taaaagacgg aagaaggctt ggatttgtct gtgacgtcga
     1801 aataaacctt gaatcgggga aaatcgaagc tattgtaatc ccaggggaca gaagatggtt
     1861 tgggcttgtt ggaaaggatg atgaatatgt tataccctgg gacaggataa aaaagattgg
     1921 ggaggatatc atcctggtgg aattggatga cagatatata aggaaacact ttgaataatg
     1981 atatggttta tttatttttg tatatagtgt attatatata ttgagcatat aaagcaattc
     2041 agactctcaa ataactgctg ggttgtggta taatttaggt acatagcgga acaagcctta
     2101 taattttatt gcaatggagg gatgtcatga agtgtccata ttgtggtttt atcgaggata
     2161 aggttattga ttcaagacct accgatgagg gatctgcaat a
//
LOCUS       DAOOJC010000414         2199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_39219_length_2199_cov_2.384328, whole genome shotgun sequence.
ACCESSION   DAOOJC010000414 DAOOJC010000000
VERSION     DAOOJC010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39219_length_2199_cov_2.384328"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..1756
                     /gene="spoIVA"
                     /locus_tag="PLA01_09685"
     CDS             278..1756
                     /gene="spoIVA"
                     /locus_tag="PLA01_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HPD01603.1"
                     /translation="MDKYDIYQQISERTQGDIYIGVVGPVRTGKSTFIKRFMDLLVIP
                     NIENEFSRGRAKDELPQSASGRTIMTTEPKFVPNEAVKIELDENVQFKVRLVDCVGYM
                     VRGAIGHMENDAPRMVSTPWFDEQIPFVQAAEIGTKKVINDHSTIGLLITTDGSITDI
                     DRDDYVEAEERVVKELKEINKPFVILLNSVNPTDADAEKLRQELESKYNVPVIGTNCA
                     QLRIEDLNNIMERVLFEFPIKEIGINIPKWIESLDENHWLRVDMINAVKEAFKGITKI
                     REIKSAMTGFDGIEFIKRAYIDNINLGTGTAYMEINEQDGLFYRVLSEMTGLEIEGEY
                     KLVSLMTELAKIKKEYEKVRDALYDVKQKGYGIVTPQMEELSLEEPEIIKQGNRFGVK
                     LRASAPSIHMIRADIETEIAPLVGTEKQSEELVNYLLKEFESEPEKLWQSNIFGKSLH
                     ELVNEGLQNKLYRMPDGAQLRLQETLQKIINDGSGGLICIIL"
BASE COUNT          760 a          321 c          508 g          610 t
ORIGIN      
        1 gggtactagt acttaaaact taagctttgg tctgtacaaa aaccagtgtc ccgtatgcaa
       61 atttaattta aatcatgccg ggatcaaaac aaaggaagtg ttttttagca tttttacagc
      121 ctggaatttg tcatgttaaa aagcactgga tgataatatt caatacagtt tgaaataaat
      181 gcatccaaaa cagtattatc gaatgctggc ttaagttttg ggtgaaaaat tcaattgaag
      241 gaaataagct tcaaataaaa gcaagggggg tttgcgggtg gataaatacg atatatacca
      301 gcagatatct gaaagaacgc agggagatat atatataggt gttgttggtc ctgtgagaac
      361 gggaaagtcc acttttatta aaagattcat ggacttattg gttataccga atatagaaaa
      421 tgaattttca agaggacggg caaaggatga actgccacaa agtgcttccg gtcgtacaat
      481 tatgacaaca gagcctaaat ttgtacccaa tgaggcggtt aaaatcgaac tggacgagaa
      541 tgtgcagttc aaggtgagat tggttgactg tgtgggttac atggtaagag gtgctatagg
      601 acatatggaa aatgatgccc cgagaatggt ttccactcca tggtttgatg aacagatacc
      661 ttttgtacag gcagctgaaa taggtacaaa gaaggtgata aacgaccact ccacaatcgg
      721 tcttttgatt acaactgacg gaagcataac ggatattgac agggatgact atgttgaagc
      781 agaagaacgg gtggtcaagg aattaaagga aatcaacaag ccgtttgtaa tacttttgaa
      841 ttcggttaat cctaccgatg ctgatgcaga aaagctcaga caggaactgg aaagtaaata
      901 caatgtgccg gtaattggta ctaattgtgc acagctcagg attgaggacc tcaacaacat
      961 aatggaaaga gtgttgtttg agtttccgat aaaggaaatc ggaataaaca taccgaaatg
     1021 gatcgaatcc ctggacgaaa accactggct cagggtagat atgataaatg ccgtaaagga
     1081 ggcatttaaa gggattacaa agatacggga aataaaaagt gcaatgactg ggttcgacgg
     1141 tatagagttt ataaaaaggg cttatattga taacatcaat cttggaaccg gaactgcata
     1201 catggaaata aacgaacagg atggcttgtt ttaccgtgtc ttaagtgaga tgacaggact
     1261 ggagattgaa ggggaatata agcttgtgtc gcttatgacg gagcttgcca aaatcaaaaa
     1321 agaatatgaa aaggttcggg atgcactgta tgatgttaag caaaagggct atggcattgt
     1381 tacacctcag atggaggagc tttctttgga agaacctgag ataatcaagc aggggaacag
     1441 gtttggtgtt aaactaaggg ccagtgcacc ctccatccat atgatccggg ctgatataga
     1501 aacggaaatt gcacctctgg tgggaaccga aaaacagtcg gaggaattgg ttaactatct
     1561 tctcaaggaa tttgagagtg agccggagaa gctgtggcaa tccaatattt ttgggaaatc
     1621 cctgcacgag cttgtgaacg aggggctgca aaacaaactg tacaggatgc ctgacggtgc
     1681 ccaattaaga ttgcaggaga cgcttcaaaa aataattaat gatggaagcg gaggtctgat
     1741 ctgcatcatt ttgtgatatg gcatgccatg gattcagcaa gcactatcat atcggttttg
     1801 atagtgcttt gtgccgtatt ttgcaatttt ttaaaaatat atttgctcaa atttaatact
     1861 tatgatatca tatatttgaa ttataaagaa taatatatgg tagtaaccat atgttaaaaa
     1921 tgatattgtt tatgcagtag acaatatgta aataatgctg tttgaagtgt acggaccaat
     1981 acttgcttta gttgttttct taggatattt tggacttaaa agtaaaatta atacagtagc
     2041 ctaaaaaagt gactaataac aagtaaaaac gcataattcg gagcataacg taatattttc
     2101 actaaaaata gtaattattt catgttgtat tttctaagat ttatttatag tataatttat
     2161 tgtataagca atatctaatt atcattaaag ggggaaaaa
//
LOCUS       DAOOJC010000415         2175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_39831_length_2175_cov_4.347642, whole genome shotgun sequence.
ACCESSION   DAOOJC010000415 DAOOJC010000000
VERSION     DAOOJC010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39831_length_2175_cov_4.347642"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..369)
                     /locus_tag="PLA01_09690"
     CDS             complement(106..369)
                     /locus_tag="PLA01_09690"
                     /inference="COORDINATES: protein motif:HMM:NF025324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
                     /protein_id="HPD01604.1"
                     /translation="MSRWVLPPAILLGGYMNNLHLYKIDLEYIKFLHNNYDNRVQYNS
                     AKGKEYNENRPYVGVLVDINGCQYFAPLEHPLRLQLKFLYYMI"
     gene            complement(583..810)
                     /locus_tag="PLA01_09695"
     CDS             complement(583..810)
                     /locus_tag="PLA01_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01605.1"
                     /translation="MFELTSDEKKALDWALNQNFGSVAAQYSKILAIALKRAMKEIEP
                     VTGEYLCVVCHKVPVDVLSGYDTCSDCASRI"
     gene            complement(810..1307)
                     /locus_tag="PLA01_09700"
     CDS             complement(810..1307)
                     /locus_tag="PLA01_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01606.1"
                     /translation="MTRKQLEKLYEVNGLTDYKLRSTEDLLRVHGIDFKEVAGYSSLD
                     DINRAIYEKFIVNIFNAWGLDSRLHLIPKGIYFVEDTEYLVKEKPEEDDYYTIAGQTI
                     YTIDRSGNKQLLHRWEDDKYKHLMKTADNPKHYLRFEYQHGTDSEGNPRNEWLHVIKE
                     GREWY"
     gene            complement(1330..1944)
                     /locus_tag="PLA01_09705"
     CDS             complement(1330..1944)
                     /locus_tag="PLA01_09705"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HPD01607.1"
                     /translation="MNTNIETAISELHKVFHKLNDVLFNSALPEPAILIQNKGNKKNV
                     LGWCSTQEIWVNKTTQERRYEINIVAEYLNRSVYEVVSTLLHEMVHLHNLVNGIKDVS
                     RGNVYHNKKFKEAAESHGLIIEYNEKLGWSLSRLNDNTKSIVDSMDLNKEAFSLARMD
                     FSNLAAGGKSSTRKYVCPQCGAIIRTSKELKVICGECLVPFELS"
BASE COUNT          637 a          474 c          333 g          731 t
ORIGIN      
        1 agtaaaatat tttaccttgg ttacttttgc cattttttac ctcctctctc gttaaatcaa
       61 gatatgatag atgttgcaat gaaggaattt aagctttaaa aagccttata tcatgtaata
      121 caaaaatttc agctgcagcc ggagtggatg ctccaatggg gcaaagtatt gacaaccgtt
      181 tatatccacc aaaacgccga cataaggacg gttttcgtta tattctttac cttttgcaga
      241 attatattgt accctgttat cataattgtt gtgcaggaat tttatgtatt caaggtcaat
      301 tttgtaaagg tgcaaattat tcatatatcc ccctaataaa atagccggtg gaagtaccca
      361 ccggctcata atccctactt taaaggcaag ggttttcctc tttcataatc cctacttaaa
      421 ggcaaggggt ttcctcttta acaatcccta ctttaaaggc aagggttttc ctctttcata
      481 atccctactt aaaggcaagg ggtttcctct ttcaaatttc aaaacttcac atatttatta
      541 tatcaaaaaa cacagaattt acaataggca gtttgcagca tattaaatcc ttgaagcaca
      601 gtcagaacag gtatcataac cgcttaaaac gtctacaggt actttatggc atactacaca
      661 cagatattca ccagtaaccg gctcaatttc tttcattgcc cttttaagag ctattgctaa
      721 aatttttgag tattgagctg caacactacc aaaattttga ttcaaagccc aatctaaagc
      781 ctttttttca tcagaagtta actcaaacat caataccact ccctaccttc tttgataacg
      841 tgcagccact cattacgtgg gtttccttcg ctatctgtac catgttggta ctcaaaacgt
      901 aaataatgct tcgggttatc tgctgttttc ataaggtgct tatacttgtc atcctcccac
      961 cgatgaagga gctgcttatt tccgctgcgg tctattgtgt agattgtctg accggcaata
     1021 gtataataat catcctcctc cggcttctct ttaacaaggt attctgtatc ttctacaaag
     1081 taaatacctt ttggtatgag gtgcaaccgg ctatcaagcc cccaagcatt gaagatattg
     1141 acaatgaatt tctcatagat agccctgtta atatcatcaa ggctgctgta ccctgcaact
     1201 tctttaaagt ctataccatg cactctcaat aagtcctctg tgctgcgtaa tttgtagtcg
     1261 gtcaatccgt taacctcata aagcttttct aactgttttc ttgtcatcgg tcaccactcc
     1321 tttataaaat taagatagtt caaaaggtac taagcactcg ccgcaaataa cctttaattc
     1381 cttgcttgtt ctgataatag ccccacactg aggacataca tattttcggg tagaagattt
     1441 tccaccggca gcaagattac tgaaatccat tcttgccaag ctaaaggctt ctttattcaa
     1501 gtccattgaa tcaacgatac ttttcgtatt gtcattaagg cgtgataagc tccaccctaa
     1561 cttttcgtta tactctataa tcaaaccatg actttcagcg gcttccttaa atttcttgtt
     1621 gtgatatacg tttcccctgc taacgtcctt aataccgtta accaaattgt gtaagtgtac
     1681 catttcatgt aacagtgtag aaacaacctc ataaacacta cggttaaggt attcggcaac
     1741 aatattaatt tcataccgtc tttcctgcgt ggttttatta acccatattt cctgtgtgct
     1801 gcaccaacct aaaacattct tcttatttcc tttgttctgg attaatatag ccggttccgg
     1861 caatgcacta ttaaaaagta catcattaag cttatgaaaa accttgtgca gctcgcttat
     1921 tgcagtttca atgtttgtat tcactctaat aacctccttg tgcagcaatg gcagaatagc
     1981 ccttatttaa cagcaatctt tctgtatctt gtagtagttc ttaaatctct cataccgtca
     2041 cccaaaatat ttattgttgg ttcatatgta agtacgtcca tccagtagat gaaatcttga
     2101 atatacgtat attatagcaa tttatacgta taaagtcaac atattttgaa agatattttt
     2161 caaaaacaga gagcc
//
LOCUS       DAOOJC010000416         2153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_40419_length_2153_cov_4.028599, whole genome shotgun sequence.
ACCESSION   DAOOJC010000416 DAOOJC010000000
VERSION     DAOOJC010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40419_length_2153_cov_4.028599"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLA01_09710"
     CDS             complement(<1..219)
                     /locus_tag="PLA01_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HPD01608.1"
                     /translation="MLSNEIPESAKWYVVHTYSGYENKVKANLEKIVENRGMQDYILD
                     IVVPMEEQIEIKDGKKKATLKKVFPGYVL"
     gene            complement(326..547)
                     /gene="secE"
                     /locus_tag="PLA01_09715"
     CDS             complement(326..547)
                     /gene="secE"
                     /locus_tag="PLA01_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPD01609.1"
                     /translation="MAQEVKVSKMTKAKGSITKFFKEISSELKKVIWPSRTQLINNTV
                     VVLVMCLLIGVIIWLLDLGFGQLTRLVYK"
     gene            complement(618..767)
                     /gene="rpmG"
                     /locus_tag="PLA01_09720"
     CDS             complement(618..767)
                     /gene="rpmG"
                     /locus_tag="PLA01_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPD01610.1"
                     /translation="MRVKITLACGECKQRNYNSMKNKKNDPDRLEMRKYCRFCRKHTT
                     HKETK"
     gene            complement(946..>2153)
                     /locus_tag="PLA01_09725"
     CDS             complement(946..>2153)
                     /locus_tag="PLA01_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPD01611.1"
                     /translation="GPVLIDICKDVTAAQAEYEPKPPKKAASIPIGVDDTDIDEAIKV
                     INNARKPVILSGGGVSIAGADKELFEFATKGKIPVTTTLMGMGSFPGTNSLFMGMLGM
                     HGTRASNTAVSESDLFIAVGARFSDRVISNVQRFAPKAKVMHIDIDPAEIGKNVVVDY
                     PLVGNIKKILKQIIAGMEEKEYSDWNKRIDEWKKLYPLKYSQDDRLHPHYIIERMYEL
                     TKGDAVITTEVGQHQMWAAQFYKYSSPRQFLSSGGLGTMGYGLGACIGAQIGKPKKKV
                     INIAGDGSFRMNCNELATAVEYKLPVIIAIFNNHALGMVRQWQELFYDGRYSSTTLDR
                     ATDFVALAEAYGAVGINVTQKEEVDSAIERALASVDTPVVINFEIDRDEKVFPIVPPG
                     APINELIEE"
BASE COUNT          590 a          538 c          327 g          698 t
ORIGIN      
        1 aaggacatac ccgggaaaca cctttttcag cgtcgctttc ttcttcccat ccttaatctc
       61 aatctgctct tccattggca caacaatatc cagaatgtaa tcctgcatcc ccctgttttc
      121 gacgattttt tcaaggtttg cctttacttt attttcatat ccggaataag tatgaacaac
      181 ataccattta gcactttcag gtatttcatt actcagcata aagcccctag tcctcctttg
      241 atctatgcga tgactgccta cagtgcctgc cacctaactt attattccaa caataatttg
      301 taggcataca ctgctttatg cattattatt tataaactaa ccgggttaac tgaccaaacc
      361 ccaaatccag caaccatatg attacaccta taagcaaaca cataaccaat accacaacgg
      421 tattgttgat aagctgtgtt ctgctgggcc aaatcacctt tttcaactcg ctgcttattt
      481 ccttaaaaaa ttttgtaatg cttccttttg cttttgtcat tttagagact ttaacttctt
      541 gagccataca aacacatctc cattatcaaa tatttttatt ttgccatcaa atcaatgaaa
      601 ccaaacatca cataaaacta ttttgtttct ttatgagtag tatgtttgcg gcaaaatctg
      661 caatattttc tcatttcaag cctgtcagga tcattctttt tgtttttcat tgagttataa
      721 tttctctgct tgcattcgcc acaagccaat gtgattttaa ctctcaactt tcacacctcc
      781 atgggtagta taaaaatata ttttatttac attatttcaa gctgtgaaga aattaattat
      841 ttcggcataa aaaaaaagac gtacacagcg aaacaatcaa taatttaaca taaatatcgc
      901 ccaaagtcaa tagatttata tcatttttct ttaaaaaact ttgttttact cctcaatcaa
      961 ttcatttata ggagcacccg gcggcactat cggaaatacc ttttcatccc tgtctatttc
     1021 aaaattaatt acaacaggag tatcaacaga ggccaatgct ctttctatag ccgaatccac
     1081 ttcctctttt tgggtcacgt ttatgccgac cgccccataa gcttcggcca atgcaacaaa
     1141 atccgttgcc ctgtccaaag tcgttgaaga ataccttccg tcatagaata attcctgcca
     1201 ctgtcttacc attccaaggg catggttgtt gaatattgca attattaccg gaagcttata
     1261 ctccaccgct gttgccaact cattgcagtt catcctgaaa cttccgtcac cggcaatatt
     1321 tattaccttc tttttgggtt tcccgatctg ggcaccaata catgccccca gtccataacc
     1381 cattgttccc aatcccccgg aggacaggaa ctgccttgga gaagaatact tatagaactg
     1441 tgctgcccac atttgatgct gtcccacctc ggtggttata actgcatcac ccttggtaag
     1501 ctcatacatc ctctcgatta tataatgggg atgtaacctg tcatcctggg aatacttcag
     1561 tggatacagt ttcttccatt catcgattct tttattccaa tcactgtact ccttctcttc
     1621 cataccggca attatttgct tcaatatctt tttaatattt cccacaagag gataatccac
     1681 tacaacattt ttccctatct ctgcaggatc aatatcaata tgcattactt tagctttagg
     1741 tgcaaacctt tgcacattgc tgattaccct gtcactgaat ctggcaccta ccgcaatgaa
     1801 aaggtccgat tcagaaaccg cagtgttgga ggctctggtt ccatgcatcc ccagcatgcc
     1861 cataaacaga ctgtttgttc ccgggaaaga tcccataccc atcaaggtgg tagttaccgg
     1921 gattttaccc tttgtggcaa actcaaaaag ctccttgtcg gctccggcaa tggacacacc
     1981 tccgccggat aatataaccg gttttctagc attgttaatg acctttattg cctcatcaat
     2041 atccgtgtcg tctactccta taggaatgct tgcagccttt ttaggaggct ttggctcata
     2101 ttcggcctgt gccgcggtta catccttgca tatgtcaatc agaacaggac ccg
//
LOCUS       DAOOJC010000417         2150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_40491_length_2150_cov_5.094511, whole genome shotgun sequence.
ACCESSION   DAOOJC010000417 DAOOJC010000000
VERSION     DAOOJC010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40491_length_2150_cov_5.094511"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(368..1564)
                     /locus_tag="PLA01_09730"
     CDS             complement(368..1564)
                     /locus_tag="PLA01_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPD01612.1"
                     /translation="MSISEEKKFILKMLEEGKISSDEAAKLIEAIENHGVKDEDDGSK
                     RQKKTSFNDEVSKVKDKLNEWKKEFKKNYSQKDFDRAVEEFTKRAEKVGKNLAYTTIG
                     FADKLVEFVSSFVDTNSFNVFGKYNATDKVFNVDNVSEGMELNVEAINGHILVKKHMD
                     SRIVIRSIVRSPEDNANDILDFSQGEGAVSLKFNKIGNISISHEIFLPAVKFKAINLV
                     TKNGKIYVEDSLSETFKAVTSNSNIDLMGVTGDNITVETKNGRISLGYIIGRNINVNT
                     NNSVIDIKQIKTANLNAVTNNGRILIENVHNYNGEPDINMKLKTSYAGIKVNMNDMEK
                     RGYRVKGETTNGDINLLIPEITYHNISKQMSKNFVEAESSGYDGYENKVNITAQTSYG
                     FIEIVK"
     gene            complement(1636..2004)
                     /locus_tag="PLA01_09735"
     CDS             complement(1636..2004)
                     /locus_tag="PLA01_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HPD01613.1"
                     /translation="MGKEVLGKCPVCNSDTYVTKISCTTCNTTIEGQFDLCRFCRLTP
                     EQKLFIDVFIKCRGNIKEVEKELGVSYPTVKNRLEDVANALGHKSEDVPDDGTKRKEI
                     LDRVYNGEISVDDALELLKD"
BASE COUNT          555 a          492 c          300 g          803 t
ORIGIN      
        1 gatattcctt gtatccaccg tttctctcga tttattttta tatgagttgg aatctgctgt
       61 tttcacttga ttgcttgcac cttgggcttt actaacctgt accgccgtag catccatgct
      121 atcaattttc ataaacaatc cctccgtgaa tttttctatc aatcagccct ccataaattt
      181 tctattatta gtatcggctt attacatcat tgagtttagc attaagtata tttttttacc
      241 aatttttatc gggtgcatta atttccctgc acaataaaat aacgtcttgc cgacgccgtt
      301 tttattcgcc gtcagcagac gttattgaat atacctgttt actaaaggat taacaaaaat
      361 ccccagtcta ttttacaatt tctataaagc cgtaagacgt ttgggcggta atgttaactt
      421 tgttttcata cccgtcatac ccgctgcttt ccgcctcaac aaaattcttg ctcatttgct
      481 tgcttatatt atggtatgtt atctcaggta tgagaaggtt tatatctcca ttggtagtct
      541 ctcccttaac cctataccct ctcttttcca tgtcgttcat attaacctta atacccgcat
      601 atgaggtctt taatttcata tttatgtccg gctctccatt ataattgtga acattctcta
      661 taagtattct tccattattg gtgactgcat tcaaattagc ggttttaatc tgtttaatat
      721 caattacaga gttattggtg ttcacattga tgttccttcc aataatgtag cccaagctta
      781 ttcttccgtt tttagtctca acagttatgt tgtcgccggt aacgcccatc aaatcaatat
      841 tgctgttgct ggtaaccgcc ttaaaggtct cggatagcga atcttccaca tagatcttgc
      901 cgtttttggt aaccaaattg atggctttaa acttaactgc aggaagaaat atttcatggg
      961 atatgcttat gttgccaatt ttattaaatt ttaaagatac agctccctca ccctgactga
     1021 aatcaagaat gtcattggca ttgtcttccg ggcttctcac tatggacctt atcacaattc
     1081 tgctatccat gtgctttttt accagtatgt gtccgtttat agcttcaaca ttaagttcca
     1141 tgccttcact gacattatca acattgaaga ccttatcggt tgcattgtac tttccgaata
     1201 cattaaaaga attggtatcc acaaaactgc ttacgaattc aacaagcttg tcggcaaatc
     1261 caatagttgt atacgcgagg ttctttccaa ccttttcagc ccgttttgtg aactcctcta
     1321 cagccctgtc aaaatctttc tggctgtaat tctttttaaa ctcctttttc cactcattca
     1381 gtttgtcctt tacctttgat acctcatcgt taaaactggt ttttttctgt cttttagaac
     1441 cgtcatcctc gtcttttact ccatggttct caattgcctc tataagttta gccgcttcat
     1501 cgctgcttat tttgccctct tcaagcatct taagtataaa ctttttttct tcactaattg
     1561 acataatgca taacgcctcc tcatctcgtg aaacttcacg gcagcttgat tacactgcct
     1621 ccaacactgc tcccatcaat cttttaacaa ttccagggca tcatccacag atatttcgcc
     1681 attgtacacc ctatcaagaa tttccttccg ttttgttccg tcatccggga catcctccga
     1741 tttgtgcccc aatgcatttg caacatcctc aagcctgttt tttaccgtgg gatatgaaac
     1801 tccaagctcc ttttccactt ctttaatatt gcctctgcac ttgataaaca catctatgaa
     1861 caacttttgc tcaggagtca gcctgcagaa cctgcacaaa tcaaactgtc cctctatagt
     1921 tgtattgcat gtagtacaac taattttcgt aacatatgtg tcgctgttgc aaacaggaca
     1981 tttcccaagt acttccttac ccatataacc actccttgat tttgttagat agaattcaat
     2041 gctttattat taaaaacgac tatactatat ttattatatt aaagcattta attattattg
     2101 tcaattattt tataaatatt attaatttta aaattaattt ttttaatgta
//
LOCUS       DAOOJC010000418         2139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_40795_length_2139_cov_3.416987, whole genome shotgun sequence.
ACCESSION   DAOOJC010000418 DAOOJC010000000
VERSION     DAOOJC010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40795_length_2139_cov_3.416987"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="PLA01_09740"
     CDS             complement(<1..1021)
                     /locus_tag="PLA01_09740"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPD01614.1"
                     /translation="MNILVINTGSSSLKYQLIDMTNESVLAKGVCDRIGLEHSFLKHT
                     KIGSDSVVVEKDLYNHKVAIQEVISALTHEKHGVIKSMSEISAVGHRIVHGGEKFKES
                     VIIDEDVVNVIRECVELAPLHNPSNIIGIEACKHIMPNVPMVAVFDTAFHQTMPRHAY
                     IYALPYEIYEKYKLRRYGFHGTSHKYVSERAAQMLGKPIESLKIITCHLGNGASICAV
                     KYGKSIDTSMGFTPLQGLCMGTRSGTIDPAVITFLMEKEKMTVKDVSDFLNKKSGVLG
                     ISGVSSDFRDIEEAAKNGDDRAKLSLEIFCYRVKRFIGDYAAIMNGVDAVVFTAGIGE
                     NNGYVR"
     gene            complement(1167..2138)
                     /gene="pta"
                     /locus_tag="PLA01_09745"
     CDS             complement(1167..2138)
                     /gene="pta"
                     /locus_tag="PLA01_09745"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HPD01615.1"
                     /translation="MERAKSNVKTIVLPESTDLRVVKAASMVQRQGIANVVLIGNKDN
                     IRELAGDIDLSGVKIEDPLNSEKFEDYANTFYELRKSKGMTIEAARETMKNPLYFGVM
                     MVKKGEADGMVAGAANSTANTLRPALQILKTAPGTKLVSAFFVMVVPNCEYGHNGAFV
                     YADSGLVENPDADQLSEIAISAAKSFKLLVGAEPQVAMLSYSSYGSAKSELTEKVIKA
                     VQLAKEKAPDLALDGELQGDAALVPEVAKSKAPGSKVAGKANVLVFPNLDCGNIAYKL
                     TQRLAKAEAYGPITQGIARPVNDLSRGCSAEDIVGVVAITSVQAQNV"
BASE COUNT          576 a          550 c          375 g          638 t
ORIGIN      
        1 ctcttacata gccattgttc tcaccgatac cggcagtaaa gactacagca tcgacaccat
       61 tcataattgc ggcatagtca cctataaacc tcttaactct gtagcagaaa atctctaatg
      121 acaacttagc cctgtcatcc ccattttttg ctgcttcctc aatatctctg aaatcactgc
      181 tcacgccgga aattccaaga acaccggact ttttattgag gaaatcgctt acatccttaa
      241 cggtcatttt ttccttttcc atcaagaagg taataaccgc cgggtcaatg gttccgcttc
      301 gagttcccat acaaagccct tgaaggggag taaatcccat ggacgtatct atggatttgc
      361 catatttaac cgcacagatg cttgcaccgt ttccaaggtg gcaggttatt atttttaaac
      421 tctcaatagg ttttcccagc atttgagctg ctctttcgga cacatactta tgggatgtgc
      481 cgtggaatcc atatcttctt aacttatact tttcgtatat ctcataggga agtgcataaa
      541 tgtatgcatg cctcggcatg gtctgatgga aagccgtatc aaacaccgca accatcggaa
      601 catttggcat tatatgcttg catgcctcta ttcctattat gtttgacgga ttgtgcagcg
      661 gagcaagctc aacacattcc ctgatgacat ttacaacatc ttcatctatt ataaccgatt
      721 ctttaaactt ctctcccccg tgaacgatac ggtgtcctac cgcagaaatc tcagacatgc
      781 tctttataac accgtgcttt tcatgggtga gggccgaaat aacttcctga attgcaactt
      841 tgtggttgta aagatccttt tcgacaacta cagaatcgct tccaatcttt gtgtgtttca
      901 gaaaagaatg ttcaagaccg atcctgtcac ataccccttt tgcgagcact gactcgtttg
      961 tcatgtcaat taactggtat ttcaatgatg aacttcccgt attaataacc agtatgttca
     1021 tttagcatct acactcctgt tcctatttat tatttgataa actataaaaa ccgtgaaacc
     1081 cactacttta aaaggctcta aagccatcaa tccatcttct tgacaccgta aagtaaaacc
     1141 cggtaataca gtttcacagt aagaaactaa acattttgag cctgtacaga agttatggca
     1201 acaacaccga cgatatcctc agcactgcat ccccgggata aatcattaac cggtcttgct
     1261 attccctgag ttatcgggcc gtaagcctcc gccttcgcca gcctttgagt gagcttgtag
     1321 gcaatgtttc cgcagtcaag attcgggaaa acaagcacat tagctttacc ggccactttg
     1381 cttcccggag ctttggattt tgcaacttca ggaaccaatg cagcatctcc ctgaagttct
     1441 ccgtcaagag ccaggtccgg tgccttttcc tttgccaact gcacagcctt tataaccttt
     1501 tctgtgagct cgctcttggc actgccatag gaagaataag aaagcatagc cacctgaggt
     1561 tctgcaccca caagcaattt aaaggactta gcagcagaaa ttgcaatttc agacaactgg
     1621 tccgcgtcgg gattttccac cagaccgcta tcggcataaa caaaggctcc gttatgacca
     1681 tattcacagt taggtacaac cataacaaaa aatgccgata ccagctttgt ccccggtgct
     1741 gtctttaaaa tctgaagtgc aggccttaaa gtattggctg tagagtttgc agcacctgct
     1801 accattccgt cggcttcacc cttcttaacc atcataactc caaaataaag aggattcttc
     1861 attgtctccc ttgcggcttc aatagtcatc ccctttgatt ttctgagttc ataaaatgta
     1921 tttgcataat cctcaaactt ctcggagttt agcggatctt caatcttaac tccggaaagg
     1981 tcgatatccc ctgccaattc ccttatatta tctttattgc ctataagcac cacatttgca
     2041 attccctgcc tctgaaccat tgaagccgcc ttgacgaccc tcaaatccgt gctttccggc
     2101 aaaacaatag tctttacatt tgattttgcc ctttcaatt
//
LOCUS       DAOOJC010000419         2135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_40895_length_2135_cov_4.075962, whole genome shotgun sequence.
ACCESSION   DAOOJC010000419 DAOOJC010000000
VERSION     DAOOJC010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40895_length_2135_cov_4.075962"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PLA01_09750"
     CDS             complement(<1..1092)
                     /locus_tag="PLA01_09750"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPD01616.1"
                     /translation="MISESVSNNLKRASWIRAMFEEGERLRKIHGADKVFDFTLGNPD
                     HEPPESLKESLKKMVLEDKPGIHRYMNNAGYEDVRQKLAGHISNETGLSLSSKHIVMT
                     CGAAGGLNVVLKSLLNPGEEVIVLSPFFAEYIFYIGNHGGKPVIVTPEKGSFKPDLDL
                     LKNSINEKTKAIIINSPNNPSGYIYDEETLLNISKLLNEKEAEYKTDIYVISDEPYTK
                     LVYDNAKIPSVLRIFKNSFVVNSFSKSLALPGERIGYIAVNPEITELDLTIESLVFCN
                     RTLGYVNAPAIFQKVIADALNEEIDIESYKKRRNLMYDSLISFGYKCIKPQGTFYLFP
                     QSPIEDDVKFVKDALKYNLLLVPGTGFGLP"
     gene            complement(1109..>2135)
                     /locus_tag="PLA01_09755"
     CDS             complement(1109..>2135)
                     /locus_tag="PLA01_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HPD01617.1"
                     /translation="NSEFTPCIIWSSESREKPLDFHAIKMSQYETADFFKSISLLLDS
                     FYTARDNINRLTQRKADLVKVLNNSISRCNKKISIHRDTLREAADREKYKLYGELITA
                     NIYCIPKNSKKATLLNYYSENSEYIVVPLDENLLPQDNAQRYFKKYAKAKTASAYAQE
                     QLKEAFNELEYLESVLQSLDTSDSLKDIEEIRQELVDGGYIMSRKKKPGRKKQESFQP
                     HVYKSSDGLNIYVGKNNIQNDILTLKLSSSNDIWLHTKNIPGSHVIIKKGKDDIPDST
                     LFQAAVLAAYHSKARNSSHVEVDYTQVRNVKKPGGAKPGMVIYNNFKTIVVTPDESMV
                     NNLKIDK"
BASE COUNT          552 a          420 c          446 g          717 t
ORIGIN      
        1 gggcaaaccg aagccggtac cgggaactaa aagcaggttg tattttaatg cgtccttgac
       61 aaattttacg tcgtcttcaa taggtgattg ggggaagagg tagaaagttc cctgaggttt
      121 tatgcattta taaccgaagg aaataagact gtcatacatg aggttgcgtc tttttttata
      181 gctttcaata tcaatttctt cgttcaatgc atcggcaatt actttttgga aaattgccgg
      241 ggcatttaca tagccaaggg tacggttgca aaatacaagg ctttcaattg tgagatccaa
      301 ctcggttatc tcaggattta cagctatata acctattctt tccccgggaa gggccaggga
      361 cttgctgaag gagtttacaa caaaggagtt tttgaatatc cttaaaacag acggaatctt
      421 tgcattgtcg taaaccagct ttgtatacgg ctcatctgat attacatata tatcggtttt
      481 atactctgct tctttttcat tcaacagttt ggaaatattt aaaagagttt cctcgtcata
      541 tatgtaaccc gaggggttgt tgggcgaatt aatgataatg gctttggttt tttcgttaat
      601 gctgtttttt agcaaatcaa ggtcgggctt gaagctgcct ttttcaggag taacaattac
      661 cggttttccg ccgtggtttc caatgtagaa tatatattcg gcaaaaaaag gcgacagcac
      721 aataacttcc tcgccggggt tgagaaggga ctttaataca acattgagcc cgccggcggc
      781 accgcaggtc ataactatgt gcttggatga aagggataat ccggtttcat tgcttatgtg
      841 gccggccagc ttttgtctaa catcctcgta tcctgcatta ttcatataac ggtgaatacc
      901 cggtttgtct tcaagtacca ttttttttaa ggattccttc aacgattccg gaggttcgtg
      961 atcaggattt cccaaagtaa aatcaaatac tttgtctgca ccgtgtattt tacgcagcct
     1021 ttctccttct tcaaacattg cccgtatcca tgaggctctc ttcagattat ttgatacact
     1081 ttccgatatc atttaaatca ctccttatct atttatcgat ttttaaatta tttaccatac
     1141 tttcatcagg ggtaactaca atagttttaa aattattata aatcaccatt ccgggtttgg
     1201 caccacccgg tttttttaca ttccttacct gggtgtagtc aacctccaca tgggaagaat
     1261 ttcttgcctt gctatggtat gctgccagta ctgctgcctg aaacagagta ctgtccggaa
     1321 tatcgtcttt gcctttcttg attataacat gggagccggg aatgtttttt gtatgaagcc
     1381 agatatcgtt agaggatgaa agcttcaatg tcaatatatc attttgaatg ttattcttgc
     1441 caacatatat atttagtccg tcggaggatt tatatacatg gggctggaaa gattcctgtt
     1501 tttttctgcc cggcttcttt tttcttgaca ttatatatcc gccgtccaca agctcctgcc
     1561 gaatttcttc aatatctttc agggaatctg aggtatcaag ggactggaga acactttcaa
     1621 gatactccag ctcgttgaag gcttctttaa gctgttcctg ggcatacgca gaagcggttt
     1681 tggcttttgc atacttttta aaatacctct gggcattatc ctggggaagg agattctcat
     1741 caagaggtac aacaatatat tcactgtttt cactgtaata gttcaataga gtagcctttt
     1801 ttgaattttt aggtatacag tatatgtttg cggtaataag ctcaccgtag agcttgtatt
     1861 tttctctgtc cgcggcttcc ctcagcgtat cccggtgaat ggaaatcttc ttgttgcaac
     1921 gggaaatgct gttgtttaag accttaacaa ggtcagcttt tctttgagtg agcctgttga
     1981 tattgtcccg ggcagtgtaa aaggaatcca gcaaaagact tatggatttg aagaaatccg
     2041 cagtttcata ctggctcatt tttattgcgt gaaaatcgag gggcttttcg cggctttcac
     2101 tgctccaaat aatgcaggga gtgaattcgg aattc
//
LOCUS       DAOOJC010000420         2128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_41078_length_2128_cov_3.476604, whole genome shotgun sequence.
ACCESSION   DAOOJC010000420 DAOOJC010000000
VERSION     DAOOJC010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41078_length_2128_cov_3.476604"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="PLA01_09760"
     CDS             complement(<1..1107)
                     /locus_tag="PLA01_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HPD01618.1"
                     /translation="MSSIFDEIIDRKNTDSLKWDYCKQRFGRDDILPMWVADMDFKSP
                     SAITKAIIRRAQRGVFGYTEVSKELTAALTGWMKKRHGWQIDDRWIEYSPSVVTSVNT
                     AILAYTNPGDKVLLQTPIYYPFYSSIKDNERELVTNPLRNNNGYYEIDFEDLEKKLAD
                     NVRLMIFCSPHNPIGRVWKIHELKEVLRLCKKYNVIIVSDEIHSDLVFKGHKHIPVAS
                     LEEKGFEKCITLVSPTKTFNIAGLSVSAAIIPDSELRRKYRSTINKTGAGLLNIFGLE
                     ALRAAYSHGEEWLEELLVYLEGNLNILEEYFQNNIPQIKVIRPEATYLAWLDCNKLPV
                     PGEKLKDFFVNEAHVGLNDGFIFGEEGYGFQRLNF"
     gene            complement(1118..2074)
                     /locus_tag="PLA01_09765"
                     /pseudo
     CDS             complement(1118..2074)
                     /locus_tag="PLA01_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
BASE COUNT          593 a          497 c          369 g          669 t
ORIGIN      
        1 gaaattcagc ctttgaaatc catagccttc ttcaccgaag ataaatccgt catttaatcc
       61 cacatgggct tcattaacaa aaaagtcttt taatttctcg cccggtaccg gtagtttatt
      121 gcaatcaagc catgcaagat atgttgcttc cggccttatt acttttattt ggggaatatt
      181 attctgaaaa tactcctcca gaatatttaa attgccttca agatacacca ataattcttc
      241 caaccactct tctccgtgag agtatgcagc cctcaacgct tccaaaccga aaatgttcaa
      301 aagaccggca ccggttttat ttatggtgct cctatactta cgcctaagct cgctatcggg
      361 tattattgca gcagatactg aaagtcctgc aatattgaag gtctttgtgg gagacaccaa
      421 tgtgatacat ttttcaaaac ccttctcttc caaagacgca accggaatat gcttatgacc
      481 tttaaacacc aaatccgagt gtatttcgtc cgaaacaata atcacgttat attttttaca
      541 aagccttaat acttctttta gctcatgaat cttccatacc cttccgattg ggttgtgcgg
      601 gctgcagaaa atcattaatc taacattatc cgcaagcttt ttttcaaggt cttcaaaatc
      661 aatttcataa tatccgttat tattcctcaa ggggtttgtt accagttctc tctcattatc
      721 cttgatactg gaataaaaag ggtagtatat gggtgtttgt aataaaacct tgtccccggg
      781 gttggtatat gcaagtatag cagtatttac cgaagtgaca acactgggac tatattcaat
      841 ccacctgtca tctatctgcc agccgtgcct ttttttcatc caaccggtta aagctgctgt
      901 gagttctttc gacacctcag tataaccgaa caccccacgc tgggctcttc taattatcgc
      961 tttagttatg gcagaagggg acttaaaatc catatcggca acccacatgg gcagaatgtc
     1021 atcccgtccg aatctttgtt tgcagtaatc ccatttcaag ctatcggtat tttttctgtc
     1081 tataatctca tcaaatatgc tgctcataaa atccccccta ttccagtgcc tgctcaaggt
     1141 cggcaattat atcctctgca tcctcaatac ctaccgaaag tcttaaaagt gtatcggtaa
     1201 ctcctatcct ctcccgcatt tcctccggta tggcggaatg ggtctgaaca acaggatagg
     1261 taatcaagct ttccacaccc ccgaggctct cagcaaacaa tactaactgc accttttcca
     1321 aggccttctc aacaatttgc acgtccttta cattaaagga aatcatggcc ccaaaaccgg
     1381 aagcttgaga ttttgagatg ttgtagcttt catgatccgg taaccctaca tagttgacct
     1441 ttgtgacatt tttatgggta caaagccact ttgcaatttt tatggcattt tcctgctgct
     1501 tttcaaggcg taaccctaaa gtctttattc ctcttaaaat cagccaactg tcaaagggag
     1561 acaacacagc cccttccgat ttttgaataa gcttgagctt ttcagccagc tcatcatcat
     1621 taactacaat aaggcctgct aaagtatcgt tatggcctcc gagatacttt gttccgctgt
     1681 gaattacaat atctgcacca agctccaagg gcctttgaaa atatggtgtt aaaaaggtat
     1741 tatccacaat caaaagcata cctcggtctt tcgcaaatcc cgatatcgcc tttatatcgg
     1801 ctaccttcat catgggattt gaaggagttt caataaaaaa agctttgctg ttgtctttca
     1861 cagcgttttc tatttcttga atcttgccgg tattgacata ggaaaactcc acactgtatt
     1921 ttttgtatat ttcctcaaac agtctgtaag tacctccata gaggtcatag gagacaatga
     1981 tatggtctcc cggtgagaaa agtgaaagta tggttgatat cgccgccata ccgctggaaa
     2041 aagcaaatcc aaatttgccg ttttcaatat ttgcggtgct tcgctatttt gtgtgggtaa
     2101 atgatatgta aaaatacaac ttttatgg
//
LOCUS       DAOOJC010000421         2115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_41446_length_2115_cov_2.266019, whole genome shotgun sequence.
ACCESSION   DAOOJC010000421 DAOOJC010000000
VERSION     DAOOJC010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41446_length_2115_cov_2.266019"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1868
                     /locus_tag="PLA01_09770"
     CDS             <1..1868
                     /locus_tag="PLA01_09770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HPD01619.1"
                     /translation="QIREMTMKKAHELYGEILPEVVEKRLDKELNSIINNGYAVMYLI
                     AQKLVAKSMADGYLVGSRGSVGSSFVATMCDITEVNPLPPHYLCPKCKYSRFFLDGSI
                     ESGADLSDENCPQCGTALRKEGHDIPFEMFLGFEGDKEPDIDLNFAGEYQPEAHKYTE
                     ELFGKGNVFRAGTIGTIAEKTAYGFIKKYYEEKGIKINNAEANRLIKGCTGIKRTTGQ
                     HPGGVMIVPDYTDIYNFTPIQRPADDPSSNITTTHFDYHSISGRLLKLDILGHDVPSI
                     IRMLEDITGFGVNNIPLDDSRTMSMFTSAEALGVNLEDIDCKVGSLGIPEFGTKFVRQ
                     MLMDTQPGTFAELVRISGLSHGTDVWLNNAQELIKSGTATLKEVIATRDDIMLFLIYK
                     GVNPRTSFKIAENVRKGRGLTPEYEAAMKEQNIPEWYIESCKKVKYLFPKAHATAYVM
                     MSFRVAYYKVYYPEAFYATYYTVKLDDFDADLFIKGKEAIRAKWIEIDKLGNNATAKE
                     KNLMTLIEVVYEMYLRGIKLLPVDLYKSDAKKFLVTKEGILPPLASLQGVGTSAAQNI
                     ACTRQESCFISMDDLRERARLSKTVIEILKNHGCLEGLPESNQLSLFSAGGNLGV"
BASE COUNT          692 a          422 c          495 g          506 t
ORIGIN      
        1 agcagataag ggaaatgacc atgaaaaaag cccatgagct ttatggagag atacttccag
       61 aggttgtgga aaagagactg gataaggaac ttaactccat aataaacaac ggctatgccg
      121 taatgtacct tatagcacaa aagcttgttg caaagtctat ggcggacggt tatctggtgg
      181 gctcaagggg ctccgtaggc tcttccttcg ttgcgacaat gtgtgatatc accgaggtaa
      241 atccgcttcc gccccattat ttgtgcccga aatgcaagta ttcccgtttt ttccttgacg
      301 gttcaataga atcaggggct gacctcagtg atgaaaattg tccccaatgc ggaacagcac
      361 tcagaaaaga gggccatgac atacctttcg aaatgttcct gggtttcgag ggagacaagg
      421 aacccgatat tgacctgaat tttgccgggg aataccagcc tgaagcccat aaatacactg
      481 aagaactatt cgggaaggga aatgtcttca gggcgggaac catcggtaca atagcggaaa
      541 agacagctta cggtttcata aaaaagtact atgaagaaaa aggtataaaa ataaacaacg
      601 ccgaagccaa cagactgatc aagggctgta ccggaataaa gagaacaact gggcagcacc
      661 cgggaggagt tatgatagta ccggattaca ccgatatata taattttact cccattcaga
      721 ggccggctga tgacccaagc tccaatataa caaccacaca ctttgactat cattccataa
      781 gcggaaggct gcttaagctg gatatactgg gtcatgatgt accgtcaatc ataagaatgc
      841 ttgaggatat aacaggcttc ggagttaata atatacccct tgatgattcc aggacaatga
      901 gtatgtttac atctgcagaa gcactgggag taaaccttga agatattgac tgcaaggtcg
      961 gaagccttgg aataccggaa ttcggaacaa aattcgtacg gcagatgctt atggacacac
     1021 aacccggcac ctttgcagag ctggtgagga tttccgggct ttcccacggc accgatgtct
     1081 ggctcaataa tgcacaggag cttataaaaa gcggtaccgc aactcttaag gaagtcattg
     1141 ccacaaggga tgatataatg ctgttcctga tatacaaggg agtaaacccc aggacctctt
     1201 tcaaaattgc ggaaaatgtg aggaaaggca gaggtttgac tcccgaatac gaagcagcta
     1261 tgaaggaaca gaatattccc gaatggtaca tagaatcctg caagaaagtc aagtatctgt
     1321 tcccgaaagc ccatgccacg gcatatgtta tgatgtcctt cagggtggca tattataagg
     1381 tatattatcc tgaagccttc tatgccacct attatactgt aaagctggac gatttcgatg
     1441 ccgatttatt cataaagggc aaagaggcta taagggcaaa atggatagaa atcgataaat
     1501 tggggaacaa tgcaactgca aaggaaaaaa accttatgac ccttatagag gttgtttacg
     1561 aaatgtatct cagaggtata aagctattgc cggtagacct ttataaatcc gatgcaaaaa
     1621 aattcttagt tacaaaggaa gggattctgc ccccactggc ttcccttcag ggagtgggca
     1681 caagtgccgc acagaatata gcgtgcacaa ggcaggaatc gtgcttcata tccatggacg
     1741 atctccggga aagagcaagg ttatccaaga cagttatcga aatactgaag aatcacggat
     1801 gcctggaagg acttccggaa agcaaccagt taagcttatt ctccgccggc ggaaaccttg
     1861 gagtttagaa taacataaaa cttgcatgta attaaaaaga gtgctataat tttgtagtgg
     1921 ataggaaaat aatgtttttc ctgcgaagat gcagcaaagt acttattaat gactttatta
     1981 acagagtggg caaatcccac tctttcgtat taatataagt aaaattactc agaatatcac
     2041 ataaaaggag agtggtcgct atggcaaaac gaaaagttga agatattgtc tacgagctgg
     2101 cgaagcctat aattg
//
LOCUS       DAOOJC010000422         2077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_42547_length_2077_cov_3.602868, whole genome shotgun sequence.
ACCESSION   DAOOJC010000422 DAOOJC010000000
VERSION     DAOOJC010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42547_length_2077_cov_3.602868"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PLA01_09775"
     CDS             <1..339
                     /locus_tag="PLA01_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPD01620.1"
                     /translation="ALIKAPVVSAAVLCGAGGDSISKLECFAQNLGLAFQIKDDILDV
                     EGSLEKLGKKTGSDAQKDKSTYVSLYGLEKSKEMLNEITEEAVMSLKDFGEKAVFLEK
                     LAKYLVNRDN"
     gene            372..812
                     /locus_tag="PLA01_09780"
     CDS             372..812
                     /locus_tag="PLA01_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HPD01621.1"
                     /translation="MEIVKGILSNKTITVPLIAWFIAQSLKVINVVLFEQKLDFTRFI
                     GSGGMPSSHSSFIVSLATVVGKTQGVDSAQFGIAVAVAVIVMYDAAGVRRAAGKQAKV
                     LNKLIFSQKGKEDFDENLKELLGHSPLEVFMGALLGILLGLGFA"
     gene            1746..>2077
                     /locus_tag="PLA01_09785"
     CDS             1746..>2077
                     /locus_tag="PLA01_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mg2+ and Co2+ transporter CorB"
                     /protein_id="HPD01622.1"
                     /translation="MDDNYKQPANGKKVKFRTNNIGSKKENKRWVFFIITLTFFLSSS
                     LSVVSSVLLEDVNLLFSLLIVLVIVIIGIVFDIIGIAVTAADESPFHAMASRKFYGAK
                     QAIKLIRNA"
BASE COUNT          590 a          330 c          515 g          642 t
ORIGIN      
        1 gcacttataa aggcaccggt ggtttcggct gcagtattat gcggtgccgg tggggattcc
       61 ataagcaaac tggaatgctt tgcccaaaac cttggccttg cctttcagat aaaagacgac
      121 atactggatg tggaaggaag tttggagaag cttgggaaaa aaaccggcag tgatgcacaa
      181 aaggacaaat ctacatatgt cagtctctac gggcttgaaa agtcaaaaga aatgcttaat
      241 gaaattactg aagaagccgt tatgagtctt aaagactttg gagaaaaagc agttttcttg
      301 gaaaaacttg caaagtatct ggttaacaga gataattaat atggttcata cgtggtggtg
      361 gaggtgtatc ggtggaaata gtaaaaggta tattgtctaa caagacgata actgtacctt
      421 tgattgcatg gtttatagca cagtcattaa aggtaatcaa cgttgttttg tttgaacaga
      481 aattggattt taccaggttt atcggttccg ggggtatgcc cagctcacat tcatcattca
      541 tagtatctct tgcgacagtg gttggaaaaa cacagggcgt tgactcagcc cagtttggaa
      601 ttgctgtggc agtggctgta attgtaatgt atgatgctgc aggagtaaga agggcggcag
      661 gaaagcaggc taaagttttg aataagctga tattttctca aaaaggcaag gaggattttg
      721 acgaaaattt aaaggaactc cttggacatt caccccttga ggtatttatg ggagcgttgc
      781 tgggtatttt gttgggtctc ggttttgcat aagggagcat tagcaagcaa aattgatata
      841 aacaaaatat tagctttatt taatagtttt aatagctttt ttaggtttta tccgttcttt
      901 aaacatttgt ttttgtatga tataatttat agccgaaagg gcaattttgg actgttatcg
      961 gtatgtttga aaacttaata cttatattta agtgctaata aaacagggtg gtgcagtatt
     1021 ctagtcagta ctaccggttc tcaagacggg cctaaaaatc cgttaaaggg cacatcgatg
     1081 aagttccttg tgctggcttg ttacgcccag tagtgggctg gtgctgggag ttaaggtttt
     1141 ggggcgatcc gcaatggcat gcgggcgttg acccctttac cgtggagacc ttgtcttgtg
     1201 gaagtaagac actgtaagct attaaaggct tgttcagggt cctctcttct acgaacaagg
     1261 attaaacctg ctatgcggta tcttaatggt gctgtgggca gagtagcctg ccttgagtga
     1321 ggttggtgga taatggtcag gtgtgtatgt atgggccaat acctatgcac tatcgcagtt
     1381 tgaaaccaat tttgcaaaag aggctaggaa tcggctagac tgttggggaa aacccctaga
     1441 ctgttcgaaa agagttacac tggggattgc agtgtggact aagtggtaat caggccccat
     1501 tgtttgttca atggctgaag gtttaaaggg aaaccgctga tttggcgaca tttcagcacc
     1561 ttctgtggaa atcatgcctg acctaagccg caaaatttac tcagtgtata tccaccctgt
     1621 gtttacatat tttttaattt ttgtagttta agtaggtgga tagattttat aagtgaagaa
     1681 aaaatatgga aggcacatga actgctgtaa tattttttct ccacgtcttt ggaggaagag
     1741 ctttagtgga cgacaattat aaacagcctg ctaacggtaa aaaggtaaag tttcggacga
     1801 acaacatcgg cagtaagaaa gagaataagc gatgggtttt ctttatcata acattgacat
     1861 tctttctgtc ttcaagtctt tcggtggtat cgtctgtttt gcttgaggat gttaatttat
     1921 tgttttcgct tttaattgtg cttgttattg ttattatcgg tattgttttc gatataatcg
     1981 ggatagccgt tacagcagca gatgaatctc cttttcatgc tatggcatca aggaaattct
     2041 atggtgcaaa gcaggcaata aagcttataa ggaatgc
//
LOCUS       DAOOJC010000423         2074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_42632_length_2074_cov_2.959386, whole genome shotgun sequence.
ACCESSION   DAOOJC010000423 DAOOJC010000000
VERSION     DAOOJC010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42632_length_2074_cov_2.959386"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLA01_09790"
     CDS             complement(<1..253)
                     /locus_tag="PLA01_09790"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPD01623.1"
                     /translation="MISQWLESLSTLISESIWLAPLLALIAGVLTSVTPCSLSTIPLI
                     IGYVGGTGNDNPKKAFRLSLVFASGMAITFTALGTAASLL"
     gene            complement(250..825)
                     /locus_tag="PLA01_09795"
     CDS             complement(250..825)
                     /locus_tag="PLA01_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPD01624.1"
                     /translation="MESHENSLKKNMPKIVIPVLIVIAIGAVFFIKARQQQDNFNKGG
                     SIVDNNGKSNDYTADFDLHVENELDLDKLKSYGLPIMIDFGADYCPPCREMKPTIERL
                     NEELKGKAIIKYVDVEKLPKIAQDYPIRVIPTQVFYDKDGNPFKPSDPDAAGMYIYSH
                     RETNEHLFTVHEGLMTEAEILTTLREMGMEE"
     gene            1100..>2074
                     /locus_tag="PLA01_09800"
     CDS             1100..>2074
                     /locus_tag="PLA01_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPD01625.1"
                     /translation="MKEELFEKYSVPKAVMTLALPTILGMLVTVFYNMADTFFVGKTN
                     DPNQMASVVLIFPVFMVLMAFGNIFGIGGGTYISRLLGLKEYEKVKNVSSFCFYACIV
                     VGTVIGILLLLFVEGVLSLSGSTENTFEFAKSYMSIIAVGTPFICLQQALGQIVRAEG
                     AARNSMIGMMIGTVVNIILDPIMIMGMGMGVVGAAVATIIGNICSVVYYIYYLTKRTT
                     VLSISIKKFKMKRDMISNILVIGIPVFVNNILFSFANLIFNNFASGYGEIVVSSIGIP
                     HRINSLPVMVMIGLSQGIQPFIGYNFSAKNYKRMNGAIRFSIACSVICG"
BASE COUNT          582 a          375 c          415 g          702 t
ORIGIN      
        1 ccagcaatga tgcagccgtg cccaaagctg taaaagttat ggccataccg gaagcgaaaa
       61 ccagcgatag tctgaaggct tttttgggat tatcgtttcc tgttcctccg acatatccta
      121 ttataagagg aatggttgaa agtgaacaag gagtcacaga ggtcaaaaca cctgcaataa
      181 gtgccaaaag cggtgcaagc caaatacttt ccgatattaa tgttgatagt gattccagcc
      241 actgacttat cattcctcca tccccatttc cctaagagtt gtaaggatct ccgcttctgt
      301 cataaggcct tcatgaacag tgaataaatg ctcgttcgtt tcacggtgtg aatatatata
      361 catgcctgca gcatcaggat ccgaaggctt gaaagggttg ccgtccttat cataaaaaac
      421 ctgtgtgggg attacccgga taggatagtc ctgagctatt ttcggcagtt tttccacatc
      481 cacgtatttt atgattgctt ttcccttcag ttcttcgtta agtctctcta ttgtaggttt
      541 catttccctg cagggagggc aatagtcggc tccaaagtct atcataatag gcagtccata
      601 ggattttagt ttgtcaaggt ctaattcatt ctcaacatga aggtcaaaat ctgcagtata
      661 atcgttggat tttccgttgt tatcgacgat ggatcctccc ttgttgaaat tatcctgttg
      721 ctgtcttgct ttaataaaga acactgcacc gatagcaata acaataagca caggtataac
      781 aatcttcggc atattttttt tcaaactgtt ctcatgactt tccatatcaa accactcctc
      841 aaaaatctgt tatattgggt atacccacaa atactccgga taaaacatcc tttctacata
      901 cgtataaaat gctccccgat tcgatggtcc tattgatttt atctttaccc tgtgttagat
      961 ttttattatt gtctcacttt taacaaggga aatcaatatg gaaatttgat atgtgtcctg
     1021 ttatgttttt tggataacat ccgaagcaca acgataaagt gattatacaa aattatgctg
     1081 ttatgaaagg atagttataa tgaaagaaga attgtttgaa aaatactctg tacccaaagc
     1141 agtaatgaca ttggcattac ccaccatact gggcatgctt gttactgtat tttataacat
     1201 ggcggatact ttttttgtgg gcaaaaccaa cgaccccaac cagatggcat cggtggtgct
     1261 tatttttcct gtatttatgg tgcttatggc cttcgggaat atttttggca taggcggcgg
     1321 tacttatata tcgcgtttgc tgggcttaaa ggagtatgaa aaggttaaaa atgtatcatc
     1381 cttctgcttt tatgcctgta ttgttgttgg tactgtaata ggtatattat tactgttgtt
     1441 tgttgaggga gtattgtctt tatccggaag taccgaaaat acttttgaat ttgcaaaaag
     1501 ttatatgtct ataattgcgg tgggcacacc ttttatttgt cttcagcaag cccttggaca
     1561 gattgtacgt gccgagggtg cggccagaaa ttctatgata ggaatgatga ttggtacagt
     1621 agtgaatatt atattggatc ctataatgat tatgggcatg ggaatgggag tagtgggagc
     1681 agctgtggct accattattg gaaatatatg ctctgttgtt tattatattt attatttgac
     1741 gaagagaacc actgttttgt ccatatcaat aaaaaagttt aaaatgaaac gggacatgat
     1801 atccaatatt ttggtaatag gcatacctgt ttttgtaaac aatattttgt ttagttttgc
     1861 gaatttgatt ttcaataatt ttgcttccgg atatggagaa attgttgttt catctatagg
     1921 tataccccat agaataaatt ctcttcccgt tatggtaatg attggtcttt ctcaaggtat
     1981 tcaacccttt attggttata acttttctgc aaagaattat aaacgcatga acggtgccat
     2041 caggttttcc attgcctgtt ccgtaatatg cgga
//
LOCUS       DAOOJC010000424         2059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_43055_length_2059_cov_3.991018, whole genome shotgun sequence.
ACCESSION   DAOOJC010000424 DAOOJC010000000
VERSION     DAOOJC010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43055_length_2059_cov_3.991018"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PLA01_09805"
     CDS             <1..221
                     /locus_tag="PLA01_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPD01626.1"
                     /translation="TAMVQPPSDKGKRLKIYYMTQASVKPPSFILFINNMELMHYSYE
                     RYLENQIRKSFGFEGTPIRFILREKEKE"
     gene            227..880
                     /gene="plsY"
                     /locus_tag="PLA01_09810"
     CDS             227..880
                     /gene="plsY"
                     /locus_tag="PLA01_09810"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPD01627.1"
                     /translation="MDWVVIKVVLAVIVGYLLGSANTSIIVGKFYGIDVRKHGSGNAG
                     MTNTLRTLGKLPALLVILGDLLKGILACVAGYCIFKAGFNAAEPGWIKEHMALGVLAG
                     GTGAVLGHNWPLYFGFRGGKGVLTTFAVILMAAPIQGLILLGIFLVVVALTRYVSLGS
                     IIAAVVFPFLTAFMGKEPEYIFFAAFVALLVILRHNSNIKRLINGTENKLGNKKGKV"
     gene            891..1910
                     /locus_tag="PLA01_09815"
     CDS             891..1910
                     /locus_tag="PLA01_09815"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPD01628.1"
                     /translation="MMRKVSIIGAGSWGIALTVLLAKAGFSVSMWSAFEDEIRMINEK
                     REHLHKLPGTLIPEGVVCTGDMEECLSDAQVLVMVVPSQITRKVARDISEYIKGNSII
                     VSCSKGLEEGTGFRMSEVIKQEIPKAQVVVLSGPSHAEEVAKDIPTTVVAASDDIKAA
                     EIVQDVFMTPKFRVYTNSDVIGVELGGALKNVIALCAGISDGLGFGDNTKAALMTRGI
                     AEISRLGVSMGANPQTFAGLTGVGDLIVTCTSMHSRNRRAGILIGQGKSLKEAVDEVK
                     MVVEGVTTTKAAYELANQEGVTMPITFEAYNVLFNGKNPKLAVSDLMMRNKKNEAMEL
                     WDKLY"
BASE COUNT          609 a          321 c          546 g          583 t
ORIGIN      
        1 ctacggcaat ggttcagccg ccttccgaca agggaaagag acttaaaatt tactatatga
       61 cacaggcatc ggtaaagcct ccttccttta ttctttttat aaacaatatg gagttaatgc
      121 attattctta cgaaaggtac cttgaaaatc agataagaaa aagttttggt tttgagggaa
      181 ctccgataag gtttatactc agagaaaaag aaaaggagtg atgtgtttgg attgggtagt
      241 tataaaagtt gtacttgccg ttatagtggg atatcttttg ggaagtgcca atacatccat
      301 tattgtgggc aaattctatg ggattgatgt tagaaagcat gggagcggaa atgcaggaat
      361 gacaaatacc ttgaggacgt tgggaaagct tccggctttg cttgtaatac taggggattt
      421 attaaaggga attttagcat gtgttgcagg ttactgtatc tttaaagcag gttttaacgc
      481 tgcggaaccc ggttggataa aggaacacat ggcccttggg gtattagccg gtggaacggg
      541 ggcagtattg gggcataact ggccgcttta tttcggtttt aggggtggaa aaggtgtact
      601 gaccacattt gccgtgatat tgatggcagc tccgatacaa ggcctgattc ttttgggtat
      661 ttttctggtg gtagtcgcat tgacgagata tgtatctctg ggctctataa ttgcagcagt
      721 ggtttttccc tttcttactg catttatggg gaaagaaccg gaatatattt ttttcgctgc
      781 ttttgtggcc cttcttgtca tattgaggca taattcgaat ataaaaagac tgataaatgg
      841 tacagagaat aaacttggaa ataaaaaggg aaaagtttag gaggttggat atgatgagaa
      901 aagtttctat aatcggggcc gggagttggg gaattgcact gacggtgctc ctggcgaagg
      961 caggattttc tgtaagtatg tggtctgctt ttgaggatga aatccggatg attaatgaaa
     1021 aaagagaaca tttacataaa cttcccggaa ccctgatacc tgaaggagtg gtgtgtaccg
     1081 gtgacatgga agaatgtttg agtgatgccc aagtattggt tatggtagtg ccttcccaga
     1141 taacccgaaa ggttgccaga gatatatctg agtatataaa gggcaatagt ataattgtaa
     1201 gctgttcgaa gggtctcgaa gagggaacgg gattcagaat gtcggaggta attaagcagg
     1261 aaattccaaa ggctcaggtt gtggtcttgt ccgggccaag tcatgcggaa gaggtggcaa
     1321 aagatattcc gactacggtt gtagcggctt ccgatgatat taaagcagca gagattgtac
     1381 aggatgtatt tatgaccccg aaattcaggg tgtataccaa ttccgatgtt attggagttg
     1441 agcttggtgg agctcttaag aatgtcattg ctttatgtgc cggaatttcc gatggcctcg
     1501 ggtttggtga caacaccaag gcggcattga tgacaagggg aattgctgaa atttcaagac
     1561 tgggagtttc catgggagca aatccgcaga cttttgcagg gctaaccgga gtgggagacc
     1621 tcattgtcac ctgtaccagc atgcatagca gaaacaggag agcaggtata ctaataggac
     1681 agggcaagtc gctgaaagaa gctgtggatg aggtaaagat ggttgttgaa ggggttacga
     1741 caaccaaagc cgcatatgaa cttgcaaatc aagaaggtgt gacaatgcct ataacttttg
     1801 aggcatacaa cgtattgttt aacggaaaga atcccaagct agcagtgtcg gacctaatga
     1861 tgagaaacaa gaagaatgaa gctatggagc tgtgggataa gttgtattaa aaatgtgtga
     1921 tattgttaac gtataaaaag cgagcggatc aaaatacatc cgcccgcttt tttttcttca
     1981 gctttgttct ccttttttgt aataataaaa gaacatagac aatatattta tattgataag
     2041 ggtactagta cttaaaacc
//
LOCUS       DAOOJC010000425         2053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_43235_length_2053_cov_4.193193, whole genome shotgun sequence.
ACCESSION   DAOOJC010000425 DAOOJC010000000
VERSION     DAOOJC010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43235_length_2053_cov_4.193193"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1915
                     /gene="pepF"
                     /locus_tag="PLA01_09820"
     CDS             107..1915
                     /gene="pepF"
                     /locus_tag="PLA01_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HPD01629.1"
                     /translation="MSEPKGKSLPKREDIDIKYKWKLEDIYSCIDDWEKDFGAVKEYV
                     SQIAEFKGTLGKSSKKLLECLTLYGELMSLCDKVFVYARMKKDEDNAISMYQGLADRA
                     SALMTEAVASTAYIVPEIISIPEDTLSAYIAEEEGLMLYRQFFKEILRQKEHILSEKE
                     EELLALSSEMSGSPREIFTMLNNADIKFPFILNENGEEIELTKGRYVMFLESKDRRVR
                     KDAFHALYSTYDKYKNTLAACLTGSIKASKFYAQTGRYNSSLEASLDSDNITADVYDN
                     LIETIDKNLPLLHRYLKLRKKALKLDDLHMYDLYVPIVEESDKNIPYEEAKKLVEEGL
                     KPMGDEYLGILREGFNNGWIDVYENQGKTSGAYSWGSYKTHPYVLLNYQGTINDVFTL
                     AHEMGHAIHSFYTNKSQPYIYSEYKIFVAEVASTVNESLLMRHLLEQTSDPQGKAYLL
                     NHYLEQFRGTIFRQVMFAQFEKIIHSKYKDGEALTTQTLNDIYLELNRKYFGSEVNVD
                     KEIAIEWARIPHFYSSFYVYKYATGFSSATAISNMILTEGKPAVDRYIKFLKSGSCDY
                     PLELLKIAGVDLSTPKPVQDALNVFEEVLCELESLI"
BASE COUNT          651 a          420 c          429 g          553 t
ORIGIN      
        1 tatgtgcttc tttctttttc tgaaactctt gctttcaaag aaattttttc gcctgaagaa
       61 aaaattttct aaccattatt ttttagcaca aaggaggctt tttgacatgt ctgaaccaaa
      121 aggaaaatca cttccgaagc gggaggatat tgatattaaa tacaaatgga agcttgagga
      181 tatttattct tgtattgacg attgggaaaa agactttggt gccgtcaaag agtatgtatc
      241 acaaatagcc gaattcaaag gcactcttgg caaaagctcc aaaaaattat tggaatgtct
      301 gaccctatac ggagagttaa tgtccctttg cgacaaagta tttgtttatg caagaatgaa
      361 aaaagatgag gataatgcca tctccatgta ccaaggtctt gcggacaggg catccgccct
      421 tatgaccgaa gccgtagctt ccacagccta catcgtaccg gagataatat caatccccga
      481 agacacattg tctgcctata ttgctgaaga agaaggactc atgctgtacc gccaattctt
      541 taaagagatt ctgcggcaga aggagcacat actctcggaa aaagaggagg agcttttggc
      601 tctgtcttcg gaaatgtccg gatcaccgcg ggaaatattc acaatgctca acaacgcaga
      661 tataaagttt ccgttcattc tgaacgaaaa cggagaagaa atagaactaa caaagggcag
      721 atatgtaatg tttctggaaa gcaaggatag aagagtccga aaagatgcat tccatgccct
      781 ttacagtacc tacgacaagt ataaaaatac ccttgcggca tgcctgacag ggagcataaa
      841 agcctccaag ttttatgctc aaaccggcag atataactct tcccttgaag cttccctcga
      901 ctccgacaat ataactgctg atgtctatga caatttgatt gaaaccatag ataagaacct
      961 tccgctgctg caccggtatt tgaagcttag aaaaaaagca ttaaagctgg atgaccttca
     1021 catgtatgac ctgtacgtac cgattgtaga agaatccgat aagaacatac cttacgaaga
     1081 ggccaaaaaa cttgtggaag agggtcttaa gcccatgggt gacgaatatc tcggaatcct
     1141 tcgggagggc tttaacaacg gctggataga tgtgtatgaa aatcagggta aaaccagcgg
     1201 tgcttactca tggggttctt acaaaaccca cccttatgtg ctgctaaact atcagggcac
     1261 aataaatgat gtttttaccc ttgcccatga aatgggtcat gcaattcact ccttttacac
     1321 aaataagtca caaccctaca tttattccga atataaaata tttgttgccg aggtggcatc
     1381 cacggttaat gagtccttac tgatgcggca tcttttggaa caaaccagcg accctcaggg
     1441 aaaagcctat cttttgaacc actaccttga acagttcagg ggaactattt tccgtcaggt
     1501 gatgtttgct cagtttgaaa aaatcattca ctcaaaatac aaagacggcg aagctcttac
     1561 aactcaaacc ctgaacgaca tttatcttga attaaaccgg aagtatttcg gaagtgaggt
     1621 aaatgtggat aaggaaatag ccatagagtg ggccagaatt ccccattttt acagcagctt
     1681 ttacgtatat aagtatgcca caggcttttc ctccgcaact gccatatcaa acatgattct
     1741 tacggaagga aaacctgctg tggaccggta tattaagttt ttaaaaagcg gaagttgcga
     1801 ttatcctttg gaactattga aaattgccgg tgtggacctt tcaacaccaa agcctgtgca
     1861 ggatgcttta aatgtatttg aggaagttct ttgtgaactt gaatctctga tatgataaaa
     1921 attaatagtt caataagtat atctaaagtc aggcccatat caggcgttga aaaaacccag
     1981 ggactttacg ccctgggttt tttgctgtgt gccaggcatg gcactaatct agctagtgaa
     2041 agtctagcca cgg
//
LOCUS       DAOOJC010000426         2046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_43427_length_2046_cov_3.593169, whole genome shotgun sequence.
ACCESSION   DAOOJC010000426 DAOOJC010000000
VERSION     DAOOJC010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43427_length_2046_cov_3.593169"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PLA01_09825"
     CDS             <1..544
                     /locus_tag="PLA01_09825"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPD01630.1"
                     /translation="VIDAGHGGVEPGAVHGGYYEKNFNLDIARRLNELLKSKGVKTYM
                     IREDDSFVGLYERAYIANSLNATLFLSIHNNAYYERFKGTETLYYPPSANSVGFNGEK
                     FAQIIQKNLIEDLNTADRGIVKRPNLVVLKATKMHASLAEIAFMTNSEDMAKLKTESF
                     RQKAAEALCDGILEALKEVD"
     gene            884..1906
                     /gene="galE"
                     /locus_tag="PLA01_09830"
     CDS             884..1906
                     /gene="galE"
                     /locus_tag="PLA01_09830"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPD01631.1"
                     /translation="MAVLVTGGAGYIGSHTVAELIEKNEEVIVVDNLEKGHRDAIISN
                     KINGGPNTGNVKLLVGDLRDKDFLKEVFSKNNIEAVIHFAAYIEVGESVSNPLKYYNN
                     NVIATLNLLTAMKAAGVSKIVFSSTAATYGEPENIPILETDKTFPTNPYGETKLSVEK
                     ALKWADNAYGIKHTILRYFNASGAHVSGCIGEDHSPETHLIPLVIQAAMGKRESIKIF
                     GNDYNTPDGTCVRDYIHVSDLANAHYLALQSLRRGEESKIYNLGNGKGFSVKEVIDVV
                     RKVTGRPIKGEEAPRRPGDPAILVASSEKIKKELNWIPKMADLETIVSTAWEWHKNHP
                     DGYNDK"
BASE COUNT          701 a          317 c          466 g          562 t
ORIGIN      
        1 ggttattgat gcaggtcatg gcggggttga gccgggagcc gtacacggag gatattatga
       61 gaagaatttt aatctggaca tagcaagaag gctgaatgag cttttgaaaa gcaaaggtgt
      121 caaaacctat atgataagag aagatgacag ttttgtggga ctttatgaga gggcatatat
      181 tgcaaacagc ctcaatgcga cactgttctt gagtatacac aacaatgcat attacgagag
      241 gtttaagggg acagagactc tgtattatcc gccgtctgcc aacagtgttg gatttaacgg
      301 agagaagttt gcacaaatta ttcagaaaaa ccttattgaa gatcttaata ctgcggacag
      361 aggaatcgta aaaagaccaa atctggtggt tctgaaggca acgaaaatgc atgcttcact
      421 ggctgaaata gcctttatga ccaacagtga agatatggcg aagcttaaga cagagagctt
      481 caggcaaaag gcagctgagg ccttgtgtga cggtatactt gaggcgttga aagaagtaga
      541 ctaaaatagt ttttgaaaca aaagttccgg gggcatctag cccccatcgc tttttgttca
      601 tttttcttcg tgaaatacga ctaataaaga tgggctttat ataagcccat ttattttatc
      661 ctccgaaagt gtattgattt ttatgtgctt aatgttcgaa tacataatta ttagatgaaa
      721 taaattatga aagatgcatt gggaagcaag ttatagggcg ttaataccgc aagaaacggg
      781 agattatatg gcatcacatg ttttcaatga ataagcttta tggtattata aataataaat
      841 tctaaatatg tattaaaaat ctacaggaaa ggttgtgttt attgtggctg tattggttac
      901 aggtggggca ggatatatag gaagtcatac ggtggcagaa ctgatagaaa agaacgaaga
      961 ggtaattgtt gttgacaacc ttgaaaaagg acatagggac gccataatct ccaacaagat
     1021 aaatgggggt cctaacaccg gaaatgttaa attattggtc ggtgacttga gggacaagga
     1081 ttttctaaaa gaggtgtttt ctaaaaacaa tatagaggcc gtaattcatt ttgcagcata
     1141 tatagaagtt ggcgaaagcg tgtcaaaccc attaaaatat tacaataaca atgttattgc
     1201 aaccttaaat cttcttacgg caatgaaggc tgcaggtgta agtaaaatag tgttttcttc
     1261 aacagctgca acttacggag aacctgaaaa cataccgatt ttagagactg ataaaacatt
     1321 tcctaccaat ccctacggag aaaccaagct ttcggtagaa aaggcattaa agtgggctga
     1381 taatgcgtat ggcatcaagc atacaatctt aagatatttt aatgccagcg gagcccatgt
     1441 tagcggttgc atcggtgagg accacagtcc tgaaacacac ctgattcccc ttgttattca
     1501 ggcagcaatg gggaaaagag aatccataaa gatattcgga aatgattata acactcctga
     1561 cggaacatgc gtaagagact atatacatgt ttccgatctt gctaatgcac attatctggc
     1621 acttcaaagc ttgcgtcggg gagaagaaag caagatatac aatcttggaa acggaaaagg
     1681 tttttctgtc aaagaggtta tagatgttgt tcgtaaggtt acagggagac ctattaaagg
     1741 agaggaggct ccaagaaggc ccggagaccc tgcaattttg gttgcatcat cggaaaaaat
     1801 aaagaaagag cttaattgga tacctaaaat ggccgatttg gagacaatag tcagcacagc
     1861 atgggaatgg cataaaaatc atccggatgg atataatgat aaataagtaa aataaaagtt
     1921 tagaaattga ttatattaca ccgctcttct gtctataatt acagtaataa caattatttt
     1981 actgtaagga gagtggttga tatgaaaaaa atattatgtc ttatattagt tcttgcaatt
     2041 actgtt
//
LOCUS       DAOOJC010000427         2043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_43517_length_2043_cov_3.435111, whole genome shotgun sequence.
ACCESSION   DAOOJC010000427 DAOOJC010000000
VERSION     DAOOJC010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43517_length_2043_cov_3.435111"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..674)
                     /locus_tag="PLA01_09835"
                     /pseudo
     CDS             complement(1..674)
                     /locus_tag="PLA01_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457738.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
     gene            complement(739..1545)
                     /locus_tag="PLA01_09840"
     CDS             complement(739..1545)
                     /locus_tag="PLA01_09840"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HPD01632.1"
                     /translation="MSERVRGNLIIIGGAEDKVGEKAILKHVAGVVKERGGGLVVLTT
                     ATEKPQEVGNNYRTVFESLGLSDIDVLNINSRDEANLDENAERIINSGGVFFTGGDQL
                     RITSILGGTKAYLALYEAYSKGVVVIGTSAGASVMSNTMVVEGRDNEPARKCTLKMAP
                     GLGFLGEAIVDQHFGQRGRIGRLLCGVAENPYMLGIGIDEDTAIRVYPDAHFEVLGSN
                     AVTVVDGSTIKISNVSELEPDEILAIAGVTLHVLPNGYGFDMAKREVLRL"
     gene            complement(1574..1699)
                     /locus_tag="PLA01_09845"
     CDS             complement(1574..1699)
                     /locus_tag="PLA01_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01633.1"
                     /translation="MTQKRWMFDIPEGFWNILNAMKNIGDSGLNTINVFSVIIGV"
     gene            complement(1839..2042)
                     /locus_tag="PLA01_09850"
     CDS             complement(1839..2042)
                     /locus_tag="PLA01_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01634.1"
                     /translation="MGWISAVISLFAYPFIFGVVGVIMGILATKNGSRAGLPLIVSSI
                     ILMGIGLMFSGVIMNYTRHYLGI"
BASE COUNT          550 a          555 c          313 g          625 t
ORIGIN      
        1 tattatccct tagaatctgc ttggtaagct gcttgtttcc caatatccac agaaatgcac
       61 cccggcaggt cagtcattga tgcctgaatc agccgggaat actttccata cccaagcctt
      121 aaaatgctgc cgtcccctat tctggtaaca ggaatgcccc gttttttggc ctctgcataa
      181 attgctgcgg tgctagggcc taattccgtc tcaacggcaa tagtctttag gtattgcaag
      241 attgcatcaa cgtcaatttc tttgttttca gcaaggctgc aaataatacc tattgcagac
      301 cttgcacact ctatggcaca ctttctgttt atatattcaa acactatata gtataatgac
      361 ggttcttcca caagtctggt tttgccatag ttcacttcat atcccagcag gttttgaatc
      421 tcaattatta catgttcgat tacatgtccg atatatgttc cttccctgag tctttcaaca
      481 aatccgccct caaagccttt ggagcaaaaa tgctttctca gacccgggaa taaagtcaga
      541 agtctttcgt taaaatcccc caggtctttt gttggagatt tataaaactc accaatatcc
      601 actaccatct taattacatg tctgtgactg tgtatatttc tccctgtaaa gcactgaata
      661 ctttgtatct gcacttctct tcctcctaaa caaacacaca taaaattctc tatacgggat
      721 tatttatagc ccttatagct aaagcctcaa tacctctctt ttcgccatgt caaagccata
      781 gccattgggc agaacatgca atgtcactcc ggcaattgcc aaaatttcat cgggttcaag
      841 ctccgacaca tttgagattt taattgtact gccatccaca accgtaacag cattgcttcc
      901 caaaacctca aaatgggcgt cgggatacac cctaatagcc gtatcctcat ctattccgat
      961 tccaagcata taagggtttt ctgccacacc gcaaagaagc cttcctattc tccccctttg
     1021 cccgaagtgc tggtccacta tggcctcgcc aaggaatccc aagcccggag ccatttttaa
     1081 agtacatttt ctggccggtt cattgtctct tccctccacc accatcgtgt tgctcataac
     1141 ggaggcacct gcactggtcc cgataacgac cactcccttt gagtaagcct catacagggc
     1201 caaataggct ttcgtgcccc caagaatact tgttattctc agttgatccc ctcccgtaaa
     1261 aaaaacccct cctgaattta tgattctttc tgcattctca tccaggttgg cttcatcacg
     1321 ggaatttata ttcaatacat caatatcact aagtcccaag gactcaaaaa ctgtcctgta
     1381 gttattcccc acttcctgag gtttctccgt ggctgtggtc agaacaacaa ggccgcctcc
     1441 tctttccttg acaacgccgg caacatgttt taaaatcgcc ttttctccga ccttgtcttc
     1501 tgcaccgcca attataatca aatttcccct tactctttca ctcattataa aattgatact
     1561 cccttaactg atgttatacg ccgattatta cactaaaaac attgatggta ttgagcccgc
     1621 tgtcccctat gtttttcatc gcatttagaa tattccaaaa gccttcagga atatcgaaca
     1681 tccatcgctt ttgggtcatt tcacttagta aaatcccact aacaaaagct ccgggggcaa
     1741 caagccccca tttctttcat ttcacttcgt gaaattccac taacaaaaaa gcaaggcatt
     1801 attctcccct tgctcttctc ttttttccca tggttttgct atatccctag ataatgtctc
     1861 gtataattca tgataactcc actgaacata agacctatcc ccattagaat tatactcgat
     1921 actattaaag gcaatcctgc cctgcttccg ttttttgttg ccagaatacc cataatcact
     1981 ccaactactc caaaaataaa cggataggca aacaaggata ttacagctga aatccatcct
     2041 ata
//
LOCUS       DAOOJC010000428         2033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_43868_length_2033_cov_3.323559, whole genome shotgun sequence.
ACCESSION   DAOOJC010000428 DAOOJC010000000
VERSION     DAOOJC010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43868_length_2033_cov_3.323559"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1963)
                     /gene="metG"
                     /locus_tag="PLA01_09855"
     CDS             complement(<1..1963)
                     /gene="metG"
                     /locus_tag="PLA01_09855"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPD01635.1"
                     /translation="MNKKTFYITTPIYYPSDKLHIGHSYTTVAADAVSRYKRLKGYDV
                     MFLTGTDEHGQKIQRKAEEKGISPKQYVDEIVAGIKDLWKLLKITNDRFIRTTDSNHE
                     KAVQQIFKKLYDQGDIYKSEYEGWYCTPCESFWTKTQLVDGKCPDCGREVELTKEESY
                     FFRLSKYQDRLIEHIETHPEFIQPVTRQNEMLNNFLRPGLEDLCVSRTSFNWGIPVTF
                     DEKHVVYVWIDALSNYITALNYLSEDDSDYRKYWPADVHLVGKEIVRFHTIIWPAMLM
                     ALGEPLPEQVFGHGWLLLEGGKMSKSKGNVVDPVVLVDKYGVDAVRYFLLREVPFGSD
                     GVFSNEALINRINSDLANDLGNLVSRTVTMIDKYFEGSIPQKRQPGDFDEDLKKVVME
                     TPSKVEELMDKLQFSTALADIWKTVSRTNKYIDETMPWKLAKDENSRERLAEVLYNLA
                     ESIRIISILIQPFMPETPEKIWHQLGISDKKATEWESAKKWGGYPEGSSVCKGDVIFP
                     RIDLQKEMEELENITVKADKEKDASKDKKDNNPGDDKKDGYITIEDFAKIDLRVAKVL
                     EAEKVEGADKLLKLKLELGNEIRQVVSGIAKNYTPESIVGKYVVLVANLKPVKLRGIE
                     SQGMILAASDDKDLVLATVDREINSGTKVR"
BASE COUNT          498 a          522 c          373 g          640 t
ORIGIN      
        1 acctgacctt ggttccgctg tttatttctc tgtcgacggt tgcaagcacc aaatctttgt
       61 cgtcggaggc agcgagaatc attccctggg actcaatacc cctcagtttg acaggcttta
      121 agtttgcaac aagcacaacg tatttaccga caatgctctc gggagtgtag ttctttgcga
      181 ttcccgaaac cacctgacgg atttcattgc ctaactcaag ctttagcttt agcagcttgt
      241 cagctccctc aaccttttcg gcttcaagaa ctttcgcaac ccttaggtca attttagcaa
      301 aatcttctat tgtaatatac ccatcttttt tatcatcacc cgggttattg tcttttttat
      361 cctttgaggc atccttttcc ttgtccgctt tcacagttat gttttcaagc tcttccatct
      421 ctttttgcaa atcaatcctc gggaaaatca catcaccttt gcagacagat gaaccttcag
      481 gataacctcc ccactttttg gcactctccc actcggtagc ctttttatcc gaaataccaa
      541 gctgatgcca tatcttttcg ggggtttccg gcataaacgg ctgtatgagt atggaaataa
      601 tccttatgct ttccgcaaga ttatacaaaa cctctgcaag cctttctctg ctattttcat
      661 cctttgccag cttccagggc atggtttcat caatatactt gttggttctg gagacagtct
      721 tccaaatgtc tgccaatgca gtactgaact gcagtttatc catcagctcc tcaacttttg
      781 acggtgtttc catcacaacc tttttgaggt cctcgtcaaa atctcccggc tgcctttttt
      841 ggggaatact tccctcaaaa tacttgtcaa tcatggtaac ggtcctgctc acaaggttgc
      901 caaggtcatt ggccaggtcc gaattaatcc tgtttataag agcttcattg gagaaaacgc
      961 cgtcggaacc gaaaggaacc tccctcaaaa ggaaatatct caccgcatca acgccgtatt
     1021 tatccaccag caccacaggg tcaaccacat ttcctttaga ctttgacatt ttgccgccct
     1081 ctaaaagaag ccatccatgt ccaaacactt gctccggcaa aggctcaccc aatgccataa
     1141 gcattgcagg ccatatgatg gtatggaacc gcactatctc cttgcccaca agatgaacat
     1201 ccgcaggcca gtatttgcgg tagtccgaat catcctccga caagtaattc agagcggtta
     1261 tataatttga aagggcatca atccatacat acaccacatg cttttcatca aaggtaaccg
     1321 gtatacccca gttaaaagag gtcctggaca cacaaaggtc ctcaaggccc ggccttaaga
     1381 aattgttgag catttcgttc tgccttgtaa cgggctgtat gaactcagga tgggtttcaa
     1441 tatgctctat cagcctgtcc tggtatttgg aaagcctgaa gaagtagctt tcttcctttg
     1501 taagttcaac ttctcttccg caatcgggac attttccgtc caccaactgg gtttttgtcc
     1561 agaaggattc acagggtgtg cagtaccagc cttcgtactc gcttttatag atatcccctt
     1621 ggtcataaag ctttttaaat atttgctgta cggctttttc atgatttgaa tcggtagttc
     1681 tgataaacct gtcattggta atcttcaaca gcttccacag gtcctttatg cctgccacga
     1741 tttcatccac atactgcttg ggggaaatcc ctttttcttc ggcctttctc tgaatcttct
     1801 gtccatgctc atccgttccg gtaagaaaca ttacatcgta gcccttaagc ctcttgtacc
     1861 tcgacactgc gtctgcagca accgtcgtat atgagtggcc tatatgtaat ttatcactgg
     1921 gatagtaaat cggtgttgtg atgtaaaagg tttttttatt cattcatgtt cctccttata
     1981 tataaagcat tataaacgta aatgctccaa tttattattg ccttccatgt ttg
//
LOCUS       DAOOJC010000429         2022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_44197_length_2022_cov_3.890188, whole genome shotgun sequence.
ACCESSION   DAOOJC010000429 DAOOJC010000000
VERSION     DAOOJC010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44197_length_2022_cov_3.890188"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PLA01_09860"
     CDS             complement(<1..712)
                     /locus_tag="PLA01_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HPD01636.1"
                     /translation="MKAELLAPAGSLEKLKMAVVYGADAVYLGGEAFGLRAYADNFTI
                     DDLKDGVKFAHERGKKVYITMNIFPHNEDLLEMPEYIKEVERIGVDAIILSDPGVLSL
                     VKETAPDMEIHLSTQANNTNWMSANFWHKQGVKRIVLARELSFNEINEIRKKTPSSLE
                     LEIFVHGAMCISYSGRCLLSSYMAGRDSNRGLCAHPCRWKYYLMEEKRPGEYFPVYEN
                     ERGSFIFNSKDLCMIEYIP"
     gene            complement(766..1404)
                     /locus_tag="PLA01_09865"
     CDS             complement(766..1404)
                     /locus_tag="PLA01_09865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003521232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HPD01637.1"
                     /translation="MICFEYINDYIRQTIKQNNGILKELETFSKENHVPIVHPEVAAL
                     LRVIGLTLKPHRILEVGTAIGYSSILLSAVLPPGGKIDTIDRYELMVDRARENIKNAG
                     LSDVINIIVGDALDVLKCLNKQYDMVFLDAAKGQYPEFLPECLRMLKSGGLLVSDNVL
                     YKGMVANDELVVRRKKTIVTRMRDYLKTLCSTDELETSILPVGDGVAISYKK"
     gene            complement(1453..>2022)
                     /gene="mltG"
                     /locus_tag="PLA01_09870"
     CDS             complement(1453..>2022)
                     /gene="mltG"
                     /locus_tag="PLA01_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPD01638.1"
                     /translation="DRKNKLDGYLFPDTYFFDPTGGPKAIIQKFLDNFNLKFKEDFYS
                     RAKELNMTVDEVIILASIIEMETANPEERAIVSSVFHNRLKSNNPDLKKLQSCATIQY
                     VLYDTQHKMKEKISDEDTKIEHPYNTYLHEGLPPGPICSPGLASIEAALYPDEESEYY
                     YFVAKGDGTHHFSKTLEEHLQAVKNYQSE"
BASE COUNT          527 a          454 c          398 g          643 t
ORIGIN      
        1 gcggaatata ctcaatcatg cagaggtcct tggaattgaa aataaaagaa cccctttcat
       61 tttcataaac agggaagtat tctccgggcc ttttttcttc catcaggtag tatttccacc
      121 tgcagggatg ggcacaaagg ccgcggtttg aatcccgacc ggccatataa ctgcttagaa
      181 ggcaccttcc ggaataggat atacacatgg caccatggac aaatatctcc agttctaaag
      241 aagagggggt tttttttctg atttcattga tttcattaaa agaaagctcc cttgccagta
      301 ctatccgttt aacaccttgt ttgtgccaga aatttgcact catccagttt gtattatttg
      361 cctgcgtact cagatgtatc tccatatcgg gagcggtttc cttcacaagg gaaagcacac
      421 cggggtcgga tagaattatc gcatcaacac cgattctttc aacttccttg atgtactcgg
      481 gcatctccaa aaggtcctca ttgtggggaa atatattcat ggttatatat accttttttc
      541 ctctttcgtg ggcgaatttt acgccatctt ttaagtcatc gattgtaaaa ttatcggcat
      601 atgccctaag accgaaggct tctccaccaa gatatacggc atccgcaccg tacacaacag
      661 ccattttcag cttttcaaga ctgcccgccg gtgcaagtaa ttctgctttc attctacttc
      721 actccaaatt cattgtcttt aaaaaaatct aagttttttg cacaatcact ttttatagct
      781 gattgcaacc ccatcaccaa cagggagtat gcttgtctca agctcatccg tactgcagag
      841 ggttttcaga tagtcacgca tccgtgttac aatggttttc tttcttctca ctaccagttc
      901 atcgttggct accattcctt tgtataacac attgtccgat actaatagcc caccgctttt
      961 gagcattctc aggcactcgg gaagaaattc cggatactga cccttggcgg catccaaaaa
     1021 taccatgtca tactgtttat taaggcattt aaggacgtcg agggcatctc cgacaatgat
     1081 gtttataaca tcggaaagac ctgcattttt tatgttttcc cgggcacggt ccaccatcaa
     1141 ctcataccgg tctattgtgt caattttccc tccaggcggc agtacggcag agagtaggat
     1201 ggaggagtat ccgatagccg taccgacttc caaaatgcgg tggggtttca gcgtaaggcc
     1261 aattactctt aaaagtgcgg caacttcagg atgcacaatg ggtacatggt tttcctttga
     1321 aaaggtctca agctccttaa gtattccatt attctgtttt attgtttgtc ttatataatc
     1381 attaatatac tcaaagcaga tcatagcata cctttcataa aaacaaggag gcgtaacgcc
     1441 cccgagaatt ttttattccg actgataatt ttttacggct tgcaaatgtt cttcaagagt
     1501 tttcgaaaag tgatgagtcc cgtctccctt ggcaacaaag taatagtatt cggattcttc
     1561 atcggggtac aatgccgctt ctattgaggc cagccccgga gagcatatcg gtccgggagg
     1621 taacccttca tgaagatatg tgttgtaagg gtgttctatt tttgtatcct catcggatat
     1681 tttttccttc atcttgtgtt gggtatcata caaaacatat tgtatagtag cacaggactg
     1741 aagctttttc aagtccgggt tgtttgattt taacctgttg tggaatacgc ttgaaacaat
     1801 tgccctttcc tctgggtttg ctgtttccat ttcaataatc gaagcaagaa ttattacttc
     1861 atcgactgtc atattcagct ctttggccct gctgtaaaag tcttccttga acttcaaatt
     1921 aaaattgtca aggaattttt gtattattgc ctttggccca ccggtcggat cgaagaaata
     1981 agtgtcggga aataagtatc catcaagctt gttttttctg tc
//
LOCUS       DAOOJC010000430         2011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_44566_length_2011_cov_2.515849, whole genome shotgun sequence.
ACCESSION   DAOOJC010000430 DAOOJC010000000
VERSION     DAOOJC010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44566_length_2011_cov_2.515849"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLA01_09875"
     CDS             <1..297
                     /locus_tag="PLA01_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD01639.1"
                     /translation="AISIYEAEINAVIHANGGIASVEIDKNKVFIKIQDKGPGIPDLE
                     LAMKEGYTTAPDVIREMGFGAGMGLPNMKRYADLLEVKTEVGKGTTVEITVYLT"
     gene            404..1759
                     /locus_tag="PLA01_09880"
     CDS             404..1759
                     /locus_tag="PLA01_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPD01640.1"
                     /translation="MSPYFHSVTLDESKCKGCTNCIKRCPTEAIRVRKSKARIINERC
                     IDCGECIRVCPYHAKKAITDSFDVIRDYKYKVAIPAPSLYGQFKSAPSRDHILTALKM
                     TGFDYVFEVAKAAEIITAETKKILSEGNFPKPLISSACPAVVRLIQVRFPSLIDNILK
                     LESPMELAAKIVKNEFSQSLKIPQEDIGVFFITPCAAKVTSIKAPYEKEKSYVDGAIS
                     INDIYLKIFTSLSDVEKVEKIQEAGFVGVRWANSGGESTALGTDKVLAVDGIHNVISI
                     LEEIENENLEDVDFLEALACQGGCLGGPLTVENMFVAKVVIKKFIDEAKKKGIEGTED
                     RKYNYDMVWTGNVEYRPIMKLDKDFEVAMKKLETLQEINKELPGLDCGACGAPSCRAL
                     AEDIVRGIANEADCIFKLREKVRSLAMQMMELEEKMPPVLDKEGNLKEKTGKRGDKNE
                     G"
     gene            1749..>2011
                     /locus_tag="PLA01_09885"
     CDS             1749..>2011
                     /locus_tag="PLA01_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPD01641.1"
                     /translation="MKVSELAEKLNMDILTEGNGSSKDIKGMYICDLLSWAMSHAQKG
                     DAWVTVHTHVNIIAVAVMADISCIIIPEGIEVEDATLKKAAQEG"
BASE COUNT          652 a          307 c          528 g          524 t
ORIGIN      
        1 gcaatttcga tatatgaggc tgaaataaac gctgttatcc atgccaatgg aggcattgcc
       61 agtgttgaaa tagataagaa caaagttttt atcaaaatac aggataaagg accgggtata
      121 cctgatttgg agttggctat gaaggaagga tacacaacgg ctcctgacgt catccgggaa
      181 atgggtttcg gtgcgggaat gggtcttccg aacatgaaga gatatgctga cttacttgag
      241 gtcaaaacag aagtagggaa gggtactact gttgagatta ccgtatacct cacatagcag
      301 tgcaaaaaaa cgggcttttc tgggaaagag agctgtttaa agtctttata catggcagga
      361 atagcatgag attcaacaag tataacaaaa aggggggttt gaaatgagcc catactttca
      421 ttcggttact cttgatgaga gtaaatgcaa aggctgtacc aattgtatta agaggtgtcc
      481 tacagaagca ataagagtga gaaagagcaa ggcaagaata ataaatgaaa ggtgcataga
      541 ttgcggagaa tgtatcaggg tttgtcctta ccatgccaaa aaggcaatta cagattcctt
      601 tgacgtaatt cgcgattata aatacaaggt tgccatacct gccccgtcgc tttacggtca
      661 gttcaaatcg gcaccgtcaa gggatcatat tcttactgct cttaaaatga ccggctttga
      721 ttatgttttc gaagtagcaa aagcggcaga gataatcacc gctgagacta agaaaatact
      781 aagtgagggc aattttccaa agccgttgat ttcttctgcg tgtcctgcgg tagtaagact
      841 tattcaggta aggtttccca gccttattga taatatattg aagcttgagt cgcctatgga
      901 gcttgcggca aagattgtaa aaaatgagtt ttcacaaagt ttgaaaattc cgcaggagga
      961 tattggggtg ttttttatta caccctgtgc agcaaaggtc acaagtataa aggctcctta
     1021 tgagaaagaa aagtcttatg tggatggtgc aatatccatt aatgatatat atttaaaaat
     1081 ctttacttct ctaagtgatg tggagaaagt ggaaaaaatt caggaagctg gattcgttgg
     1141 agtaagatgg gccaattccg gaggggaaag cactgccctg ggtactgata aagtacttgc
     1201 ggttgatgga atacacaatg tcatttcgat tcttgaagaa atcgagaatg agaatctgga
     1261 ggatgtcgat tttttagagg ctttggcatg tcagggtgga tgtcttgggg gtccccttac
     1321 tgttgaaaac atgtttgttg ccaaggtcgt gattaaaaag tttattgatg aagcgaagaa
     1381 aaagggtatt gaaggtacag aagacagaaa atataactat gatatggtgt ggaccggaaa
     1441 tgttgagtac agaccaatta tgaagctgga taaagacttt gaggtcgcta tgaagaaact
     1501 cgaaacactg caggaaataa acaaagagtt gccggggctg gattgcgggg catgcggtgc
     1561 acccagttgc agggcactgg cggaagatat agtaagaggc attgctaatg aggccgattg
     1621 tatattcaag ctcagagaaa aggtacgaag tcttgcgatg cagatgatgg agttggaaga
     1681 aaagatgcct ccggttctgg ataaggaagg aaatttgaag gaaaaaacag ggaaaagagg
     1741 ggataaaaat gaaggttagt gagctagcgg agaaattaaa tatggatata ttgacagaag
     1801 gaaacggctc ctcgaaggat attaagggca tgtatatctg tgacttgtta agctgggcca
     1861 tgtctcatgc ccaaaaaggg gatgcatggg ttacggttca tacccatgta aatattattg
     1921 ctgttgctgt tatggcagat atttcatgta ttattattcc cgagggaatt gaagtagagg
     1981 acgcaacctt gaaaaaagca gcacaagagg g
//
LOCUS       DAOOJC010000431         2010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_44602_length_2010_cov_2.832737, whole genome shotgun sequence.
ACCESSION   DAOOJC010000431 DAOOJC010000000
VERSION     DAOOJC010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44602_length_2010_cov_2.832737"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..510
                     /locus_tag="PLA01_09890"
     CDS             208..510
                     /locus_tag="PLA01_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01642.1"
                     /translation="MGAGKTKTVSDGQGYNYIIDNAGNIRRDTSYPDSWMQPMWLQPP
                     GFVKITNRHDYGDVFYNIHGYRRDSGQMGIPIVPIPGGGIPVPAPAPMPMPIPVFP"
     gene            557..856
                     /locus_tag="PLA01_09895"
     CDS             557..856
                     /locus_tag="PLA01_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01643.1"
                     /translation="MNNNVKAKIYWISEKNGGRKNIPNSRNYSTVACFEDIKDQFSTE
                     AWSVVIDLENAQKESNWVIADFRFLVESAPCNLLYTGSKFQLFEGRRLVAIGEIL"
     gene            1035..1424
                     /locus_tag="PLA01_09900"
     CDS             1035..1424
                     /locus_tag="PLA01_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01644.1"
                     /translation="MSTEKDSMLRVRLTQRQSDELDAIIGELQAQMPEASVTTSSIAR
                     YALEKYVSDHIAKRDGTKIFIEVSTADATDEDIKNLYDLISRLFDETKENHSPMVHYM
                     VGEILEPVMMKMASLMKPKKPEVRDGE"
     gene            1417..1554
                     /locus_tag="PLA01_09905"
     CDS             1417..1554
                     /locus_tag="PLA01_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01645.1"
                     /translation="MSKKQYTVRCPHCNHRVFDADYADVEIKCPGCRKVFEVKLEKKA
                     G"
     gene            1682..>2010
                     /locus_tag="PLA01_09910"
     CDS             1682..>2010
                     /locus_tag="PLA01_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HPD01646.1"
                     /translation="MTMMDAALKYAEANIPVMPLHWICEDGSCSCKKGQQCDSKGKHP
                     LYTGWYKNSTTDVEQIKKWWTKTPNANIGIPTGEKSGWLVLDVDDGGDETLSALEATH
                     GKLPDTVT"
BASE COUNT          684 a          329 c          476 g          521 t
ORIGIN      
        1 tgaaaaacag aatttaatat gatattatga aaaaagcaaa tacctatcta ttataaattt
       61 ataagatggg aaaggcttga tggaattggg cctgcacgtg ctgcagaatg tagtgatcta
      121 aataaactaa aattaaaggt attagaagta aatgaccgta atgacgacga tgatttaaaa
      181 ccaggtggag gtagcagttc tggaggtttg ggagcaggga agacaaaaac tgtatcagac
      241 ggtcagggat ataattacat aatagataat gcgggaaata taagacgtga tacttcatat
      301 ccagatagct ggatgcaacc aatgtggctg caaccgccag gttttgttaa aattactaac
      361 aggcatgact atggagatgt tttttataac atacatggtt atagaagaga cagtggtcaa
      421 atgggcatac ctatagttcc tattccgggt gggggtatac ctgtacctgc gcctgcacca
      481 atgccgatgc caataccagt atttccttaa gcattacgat atcactatat acggtgttaa
      541 gaggtgaata tacaatatga acaataatgt taaagcaaaa atttattgga tatcagaaaa
      601 aaatggagga cgtaaaaata ttccaaacag tcgtaactat tcaacggttg catgttttga
      661 agatattaag gaccagtttt ctacagaagc ttggagtgtt gtaattgatt tagaaaatgc
      721 tcaaaaggaa tcaaattggg ttattgcgga ttttagattt ttagttgaaa gtgctccttg
      781 taatttgtta tacacaggca gtaaatttca gttatttgag ggaagaagat tagttgctat
      841 cggagaaatt ttataaaatt aactgacaga aggccatctg acaaatggtt cactaagtaa
      901 attatacaat aattgacatt atcattacct ggttaccaat aaaacggtag ccgggttttc
      961 tttttatcca tgcttgacaa aatgtaatac gatgtgatac aataaaacca acaagaaata
     1021 caggaggtgc aataatgtct acagaaaagg atagtatgct acgggtaagg cttacacaaa
     1081 ggcagtcgga tgagttggat gctattattg gtgagcttca agcgcaaatg ccggaagcaa
     1141 gtgttaccac atcaagcata gcaagatacg ctctggagaa gtatgtgagc gaccatattg
     1201 ccaagcgtga cggtaccaag atttttattg aagtgtctac cgcagatgcc acagacgagg
     1261 acataaaaaa tctctacgac cttatttcca ggctgtttga tgaaacaaag gagaaccact
     1321 caccaatggt tcattacatg gttggagaga ttttagagcc tgtgatgatg aagatggcaa
     1381 gcctcatgaa gcctaagaaa ccggaggtga gggatggtga gtaagaaaca gtacacagtc
     1441 cgctgccccc attgcaatca cagagtattt gatgccgatt atgcagatgt agaaatcaag
     1501 tgccccggat gccggaaagt ctttgaagtg aagctggaga aaaaggcggg gtaaaaaagt
     1561 gaatagatct ggcacagagc cacaaaggga gcgaatgact cacctataga gcctggcaga
     1621 tagtcttaaa aactatttgt caggctctat ttatgttctc ggaggaggtg agaatttgaa
     1681 agtgacaatg atggacgcag cattaaaata tgcagaagcc aatatcccag ttatgccttt
     1741 gcactggatt tgtgaggatg gctcctgctc ctgcaagaag gggcaacaat gtgacagcaa
     1801 gggaaagcat ccgctatata ccggctggta caagaattcc actactgatg ttgagcaaat
     1861 aaagaaatgg tggacgaaaa cacctaatgc caatattggc attcctacag gagagaagtc
     1921 cggctggctg gtacttgatg tggatgatgg tggtgatgaa actctatcgg ctcttgaagc
     1981 aacacatgga aaacttccgg atacggttac
//
LOCUS       DAOOJC010000432         2008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_44675_length_2008_cov_3.465438, whole genome shotgun sequence.
ACCESSION   DAOOJC010000432 DAOOJC010000000
VERSION     DAOOJC010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44675_length_2008_cov_3.465438"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1611)
                     /locus_tag="PLA01_09915"
     CDS             complement(<1..1611)
                     /locus_tag="PLA01_09915"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPD01647.1"
                     /translation="MDESKNIAKTYDPKQVEERLYENWMEKGYFTPVIDKNKEPYTIV
                     IPPPNITGQLHMGHAMDNTIQDILIRWRRMQGYCTLWLPGTDHASIATEAKIVETLAK
                     EGKTKYDLGREKYLEMAWDWKHEYGGRILNQLKKLGSSCDWTRERFTLDEGCSDAVLE
                     VFTRLYEKGLIYRGERIINWCPKCKTSISDIETIYDTSKGNFWHIKYPVKDSDEYIEI
                     ATTRPETMLGDTAVAVNPNDDRYRHLIGKTLILPLMNKEIPVIEDEYVDMEFGTGAVK
                     ITPAHDPNDFEVGMRHNLPMPRILNDDGTINSLGGKYEGLDRYEARKQIVKDLEDQGL
                     LVKTKEHEHNVGHCQRCSTTIEPLLSKQWFVKMKPLAEPAIEVVRDGRVKFVPERFTK
                     IYYNWMENIQDWCISRQLWWGHRIPAYYCEDCGEVIVSKTAPESCNKCGGKLKQDEDT
                     LDTWFSSALWPFSTLGWPEKTEDLEYFYPTSVLVTGYDIIFFWVARMIFSAMENMQEE
                     PFRHVLIHGIIRDSEGRKMSKSLGNGIDP"
     misc_binding    complement(1675..1886)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          471 a          518 c          370 g          649 t
ORIGIN      
        1 agggtctatc ccgtttccga gggatttgct cattttcctg ccttcagaat ccctgattat
       61 accatgaatc aggacatgcc tgaaaggctc ttcttgcata ttctccattg ctgagaaaat
      121 cattctggca acccagaaga atatgatatc atatcctgta accagtacgc ttgtaggata
      181 gaaatactca agatcctcag tcttttcagg ccagccaagg gtggaaaaag gccacaaggc
      241 agaactgaac caggtatcca aagtatcttc atcctgctta agctttcctc cgcatttgtt
      301 gcaggattcc ggtgctgttt ttgatacaat tacttcgccg cagtcctcac agtagtaagc
      361 gggtatcctg tgcccccacc acagctgtct tgaaatacac caatcctgaa tgttttccat
      421 ccaattataa tagatcttcg tgaacctctc cggcacgaat tttactctgc cgtcccttac
      481 aacctctatt gccggctcag caaggggctt catcttaacg aaccactgct ttgaaagcaa
      541 aggctctatt gttgtgctgc acctttggca gtgccccaca ttgtgctcat gctcttttgt
      601 cttaaccaac agtccctggt cttccaggtc tttaactatc tgcttccttg cttcataacg
      661 gtccagccct tcatatttgc cgcccaggct gtttattgtt ccgtcatcat taagtatcct
      721 cggcatcgga agattgtgcc tcatgcctac ttcaaagtca ttggggtcat gagccggtgt
      781 tatcttaact gctccggtcc cgaactccat atcgacatac tcatcttcta ttaccggtat
      841 ttctttgttc ataagcggca gtatcaatgt cttacctata aggtgcctgt acctgtcgtc
      901 atttggatta actgctacgg cagtatctcc cagcattgtc tccggccttg tagtggcaat
      961 ttctatatat tcgtcactgt cctttacggg atactttata tgccaaaaat ttcctttcga
     1021 agtgtcataa atagtctcga tatccgatat ggaggtcttg cacttggggc accagtttat
     1081 tatcctctct cccctgtaaa taagcccctt ttcatatagc ctggtgaaaa cctccaaaac
     1141 tgcatctgaa cagccttcat ccagggtaaa cctttcacgg gtccagtcac aggagcttcc
     1201 aagcttttta agctggttca gtattcttcc gccatattca tgcttccagt cccaagccat
     1261 ctcaagatat ttctctcttc ccaaatcata cttcgttttt ccctcttttg ccagagtctc
     1321 aactatctta gcctctgtag ctatgcttgc atgatccgta cccgggagcc agagtgtgca
     1381 gtatccctgc atcctcctcc accttatgag tatatcctgt atggtattgt ccattgcatg
     1441 gcccatatga agctgtccgg taatattcgg cggaggtatt actatggtat agggctcttt
     1501 gttcttatcg atcaccggag taaaatatcc tttttccatc cagttctcgt ataatctctc
     1561 ttctacttgt tttgggtcat aagtttttgc tatgtttttt gattcgtcca tgttaaatcc
     1621 tcctcacagt tgaatttgaa ttgtgccgta ttaaaataaa aaacccccgc ccatgttaag
     1681 ggcgaaggtt attcgcggta ccaccttatt tccggcaaac accggctctt tgaactgtaa
     1741 cgggcagacc cgcctgatgc tactctattc gcagcaggag ctcagaagct accttcagca
     1801 gtgccgtacc tggaaaacct ttcagcctgt gggttctcct ctctaaaagg cggttagtgc
     1861 ctactcctct tcctcattgc tgataattaa taacatatta tttatatata ttattagagt
     1921 tgtgcggatt tgtcaatatt attttttaat cagattttaa cataaaatgc agcactaaat
     1981 gccgcctctg ttaagcaact gatttcat
//
LOCUS       DAOOJC010000433         2007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_44708_length_2007_cov_4.433914, whole genome shotgun sequence.
ACCESSION   DAOOJC010000433 DAOOJC010000000
VERSION     DAOOJC010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44708_length_2007_cov_4.433914"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..427
                     /locus_tag="PLA01_09920"
     CDS             305..427
                     /locus_tag="PLA01_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01648.1"
                     /translation="MAVCILMLRMLFRGRGDLMVERFYALCQGFSCPWQRALCL"
     gene            456..>1019
                     /locus_tag="PLA01_09925"
     CDS             456..>1019
                     /locus_tag="PLA01_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD01649.1"
                     /translation="MNFLDKYKEDFKEYDLMEKDFIDDDKIWEQLNKWETPGRDDVRS
                     VLKKAEKKIRLEPEEMAILIQNKDEETINEMYALASKLKREVYGDRIVFFAPLYISNK
                     CANNCKYCGFRRENKMIERRTLTMDEISQEIRIMTGEGQKRTVLVYGESPETDIDFMC
                     DSIRQVYNTKCGNGEIRRANVNCAPLSR"
     assembly_gap    1020..1029
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1030..1903
                     /gene="hydG"
                     /locus_tag="PLA01_09930"
     CDS             <1030..1903
                     /gene="hydG"
                     /locus_tag="PLA01_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPD01650.1"
                     /translation="KLKEVGIGTFQVFQETYHHETYRKMHPEGTIKWHYRWRLYAQDR
                     ALDIGLDDVAIGVLFGLYDWRFEAMGLLYHTIHLEEKYNGVGPHTISFPRIEPAIGTS
                     YASNPEYAVSDEDFKKLVAILRLSVPYTGLILTAREKPEVRREVIPLGVSQIDAGTRI
                     GVGGYKKSMGNMMPDKEQFQLGDVRSLDDVIRETCSMESLPSFCTACYRAGRTREHFM
                     EVAKSSFVHNFCMPNAILTLKEYLLDYACDETKKAGEKAINKYISALEDAKTKQFVVD
                     ALERMEKGERDIRV"
BASE COUNT          627 a          321 c          494 g          555 t
ORIGIN      
        1 acaattctta catttcttta cattctaaat tattaagtca ttttgcaaat tcctagaatt
       61 attttagatt attatattta ttattttatt attatcttgt ttgcatattc ctgttattat
      121 ctatattgat ataaaatatt agcatgctta tacataagtt tgcttattga cagtgcttcg
      181 ttatgagcgt aatatataag taaagagtct tattgataga ctgtatacaa attaatactg
      241 caaaaattta attccgcttt caaacaaggc ttgactccaa gagcataagc ttgaggagga
      301 gagtatggcg gtctgtattc tgatgctgag gatgcttttc cggggcagag gagatttgat
      361 ggtagagagg ttttatgccc tttgtcaagg attttcctgt ccatggcaaa gggctttatg
      421 tttgtaataa cgggataaaa aggagggttt ttaaaatgaa ttttctcgac aagtacaaag
      481 aggactttaa agaatatgat ttgatggaaa aagattttat cgatgatgat aaaatatggg
      541 aacaacttaa caaatgggaa actccgggca gagacgatgt gagaagtgta ctgaaaaagg
      601 cggagaagaa gattcggcta gagcctgagg aaatggctat ccttatacaa aacaaggatg
      661 aagaaaccat taacgaaatg tatgcccttg ccagcaaatt aaagagagaa gtctacggag
      721 accgtattgt cttttttgca cccttgtata taagcaacaa gtgtgctaat aattgcaagt
      781 attgtgggtt cagaagagaa aataaaatga ttgagcgaag gactctcacc atggatgaga
      841 tatcacaaga gataagaatc atgacaggtg agggtcaaaa aaggactgtg cttgtttatg
      901 gagaatctcc cgagacggat atagatttta tgtgtgacag catccgccag gtatataaca
      961 cgaaatgcgg aaacggtgaa atacggcggg caaatgtgaa ctgtgctccc ctgtcccggn
     1021 nnnnnnnnng aaactgaagg aagttgggat aggtacgttc caggtgttcc aggaaactta
     1081 ccatcacgag acatatagaa agatgcaccc ggaaggaaca attaagtggc actaccggtg
     1141 gaggctttat gcccaggaca gggcattgga tatcgggctg gatgatgtcg ccatcggtgt
     1201 attgttcgga ctttatgact ggaggttcga ggctatgggg cttttatacc atacaataca
     1261 ccttgaggaa aagtataacg gtgttggtcc tcatacaatt tcgttcccga ggatagagcc
     1321 tgctattggg acctcttatg catcaaaccc ggaatatgcc gtaagtgatg aggatttcaa
     1381 gaagcttgtg gcaattctga ggctgtcggt gccctataca ggattgattc ttactgcacg
     1441 ggaaaagccc gaagtaagaa gagaggttat tcccttggga gtttcacaga ttgatgcggg
     1501 aaccagaata ggagtgggag gatacaagaa atctatgggc aatatgatgc cggataaaga
     1561 acagttccag cttggagatg tccgaagtct tgatgatgta atacgggaaa catgctcaat
     1621 ggaaagtctt ccttcctttt gtacggcatg ctacagggca ggcagaacca gggagcattt
     1681 tatggaggtt gcaaaatcaa gttttgtgca taatttctgt atgcccaatg ccatcctgac
     1741 acttaaggag tatcttttgg attatgcttg cgatgagaca aagaaggccg gcgaaaaggc
     1801 tataaataaa tatatatccg ctcttgaaga tgcgaaaaca aagcagtttg ttgttgatgc
     1861 attggagaga atggaaaaag gtgaaaggga tatccgggtt tgacggaata ccggtataaa
     1921 aggctcaaac aatgaccgtt tgagcctttt tatgactgtg tgccaggcat ggcactaatc
     1981 tagctagtga aagtctagcc acgggta
//
LOCUS       DAOOJC010000434         2007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_44709_length_2007_cov_4.412910, whole genome shotgun sequence.
ACCESSION   DAOOJC010000434 DAOOJC010000000
VERSION     DAOOJC010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44709_length_2007_cov_4.412910"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1797
                     /locus_tag="PLA01_09935"
     CDS             <1..1797
                     /locus_tag="PLA01_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HPD01651.1"
                     /translation="NSGALNSYQEDMLTPLDFGIYSNNNLTIQGQNAKINGSTHANNV
                     FKTTAFTVAITESCSASSFELISPYMDIDNFINIEVPIEMPYFHSKLINEAITSDQVY
                     DPEDFKGPGGNRRPMPGQTGIYIEYKQAQQRFEITGNGTLVIDSCMYFKSDLFISLNE
                     TNNVNEGFLIADGKITIQGRDIYPRGPNDKLYVYSINGNIEFQTSGCTINGIAYAPGN
                     PSNPGSGNIIFSGNNNTINGSIVGNDLNFDAAGLTVNHVENQFDSVERKYFEGSSYLN
                     SVKDAARAFVNLFKGSKTKMSVIQYSESANNNDFRLYDLSSETEANQLKESINNIVPG
                     TSGYSNMGDGMRRAYHILNGSSDSQAEKYIIVLAGSEPNRWTSSSNVILEPKTNNGDA
                     QYLKPDELLYKSVDYAEDIGNIIAGSNIEAFFIDFSIDGIGSVLESIAVSSGAKVITE
                     TGKHYYRADNSLELLHIFDGIHSYAVYDVSLNNVVYEEILPIGVVALEVPEWMSIETL
                     EIDGITRYKVSGTLDSIPLNYDGGKYTFSVDGFDIKAQFLKPGEVLFDGKDAKMVFKV
                     DYIDYDGNLKSENIEIHPQNMTVKVTMKIDIS"
BASE COUNT          670 a          347 c          408 g          582 t
ORIGIN      
        1 aactccgggg cactgaattc ataccaggag gatatgttga ctcctctcga ttttggaata
       61 tattcaaaca ataatttaac tattcagggt caaaatgcaa aaattaacgg aagtactcac
      121 gcaaataacg tatttaaaac cactgccttt actgtagcaa ttaccgaaag ctgttctgct
      181 tcttcatttg agctcataag tccatacatg gatatagaca atttcattaa cattgaagtg
      241 cctattgaga tgccgtattt tcactcaaaa ctgattaatg aggcaattac aagtgatcaa
      301 gtttacgatc ctgaagactt taaaggtcca ggaggaaacc gccggcctat gcctggacaa
      361 accggtatat atatcgagta taagcaagcc cagcagcgtt ttgagattac aggtaacggt
      421 actttagtta tagactcctg catgtatttt aagtcggatc tatttatatc cttgaatgaa
      481 acaaacaacg tcaatgaggg atttcttatc gcagatggga aaataaccat acagggaaga
      541 gacatatatc ccagaggacc taacgacaaa ttgtatgtgt actccattaa tggtaatatt
      601 gaatttcaga ccagtggatg tacaatcaac ggaatagctt atgctccggg taatccgtcc
      661 aatcctggct caggtaatat tattttttca ggtaataata acactataaa cggctctatt
      721 gtaggaaacg accttaattt tgatgctgcc ggtcttacag taaatcatgt agaaaatcaa
      781 tttgattctg tggaaaggaa atatttcgag ggctcaagtt acttgaattc tgttaaggat
      841 gcagccagag cttttgttaa tctgtttaaa ggctcaaaaa ccaaaatgtc tgttatacag
      901 tactcggagt ctgcaaataa taatgatttc agactatatg acttgtcttc ggaaacagag
      961 gcgaatcaac tgaaggagag tattaataat attgtacccg gaacctctgg atacagcaat
     1021 atgggagacg gaatgagaag ggcttaccac attcttaacg gttcttccga cagtcaggcg
     1081 gaaaaatata taatcgtact tgctggttcc gaacctaaca gatggacaag ttccagcaac
     1141 gtaattttgg aaccgaaaac caataatggt gatgctcagt atttaaaacc cgatgaatta
     1201 ctatacaaat ccgtggatta tgcagaggat attggaaaca ttattgccgg aagcaatatt
     1261 gaagcgtttt tcattgactt ttcaattgac ggtattggga gtgtcttaga gagtattgca
     1321 gtatcaagcg gtgcaaaggt aataactgaa accggcaaac actattacag agccgataac
     1381 agtttagaac tcttgcatat ctttgacggt attcattcat atgctgttta tgacgtttca
     1441 cttaacaatg tagtatatga agaaatactg ccgataggag ttgtagcact cgaagtgcct
     1501 gaatggatga gcatagagac tttagagatt gacggaataa caagatataa ggtttcggga
     1561 accttagaca gtatacccct aaactatgac ggcggaaagt atacctttag tgttgatggc
     1621 ttcgatataa aggctcagtt cttaaaaccc ggtgaggtat tgtttgacgg caaggatgca
     1681 aagatggtct ttaaagttga ctatatagat tatgatggta acctcaaatc tgagaatatc
     1741 gagatacatc cgcagaatat gacggtaaaa gtgacaatga agattgatat aagctaacca
     1801 aagggtgcat tgattcttaa acatttttct tagcataaag tataatactc acaggcaaaa
     1861 aagagtcagg gtaaaaatcc ctggctcttt ttatgtgaaa tttttgcggt gaagtctcac
     1921 aacctcccaa aatgcatatc ttaatattag gtttcaatca ataccagtta taattttatt
     1981 tcaatggagg atgtcaatta tgtcaaa
//
LOCUS       DAOOJC010000435         2006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_44747_length_2006_cov_3.602768, whole genome shotgun sequence.
ACCESSION   DAOOJC010000435 DAOOJC010000000
VERSION     DAOOJC010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44747_length_2006_cov_3.602768"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PLA01_09940"
     CDS             complement(<1..544)
                     /locus_tag="PLA01_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPD01652.1"
                     /translation="MNILVTGGAGFIGRWVVKRLLDDGHKVWALDDLSNGQVSNIEEF
                     FDNPNFVEFIEGDIKDIDTLRRLFEYKFDICYHLAASINVQDSIDDPGTTFQNDTVGT
                     FNVLEQCRKHNTKIVFMSTCMVYDRANDENGITEEHTVKPASPYAGSKIAGENMVLSY
                     WYAYKLPAVVIRPFNTYGPMQ"
     gene            complement(560..1729)
                     /gene="pseC"
                     /locus_tag="PLA01_09945"
     CDS             complement(560..1729)
                     /gene="pseC"
                     /locus_tag="PLA01_09945"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HPD01653.1"
                     /translation="MDKRLEKKFIPYGRQWIDEDDIKAVTEVLRGDYLTTGPKIKEFE
                     DKLAQCTGAKYAVAVSNGTAALHAACFAAGIKEDDEVITSPITFAASANCVLYLGAKP
                     IFADINSETYNIDPDEIRTKINEKTKAIIPVHFTGQPCDMDEIQKIAQEYNLTIIEDG
                     AHALGAEYKGRKIGSIGDMTTFSFHPVKNITTGEGGAITTNDEELYKKLTLFRTHGIT
                     RDKDVLLKDEGPWYYEQQCLGYNYRITDIQAALGISQLDKLERFLAQRRDFVGRYNEA
                     FGGAEAIETPYQLENTNSAWHLYIIKLKLERLNCGRRQIFEELIERGIGVNVHYIPVY
                     YHPYYRQLGYNKGLCPKAEELYERIITLPLFPKMDRDDIEYVISNVREVIQNHLR"
     gene            complement(1816..>2006)
                     /locus_tag="PLA01_09950"
     CDS             complement(1816..>2006)
                     /locus_tag="PLA01_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01654.1"
                     /translation="DEVVRVTPDCPLTDSVIFNDKIDFYMSNNYKLVTDSGISFDQRH
                     IQGLGYRGVFLQYIGRRL"
BASE COUNT          543 a          472 c          339 g          652 t
ORIGIN      
        1 tttgcatggg gccataggta ttgaagggtc ttataaccac tgccggcagc ttataggcat
       61 accaatagga aagcaccatg ttttcccctg caattttgct gcccgcataa ggagaagcag
      121 gttttacggt atgttcttcg gttattccgt tttcatcgtt tgccctgtca tacaccatac
      181 aggtgctcat gaacacaatt ttggtattgt gttttctgca ctgctccagt acattaaagg
      241 ttcccaccgt gtcattttga aatgtcgtgc caggatcatc gatgctgtcc tgcacattaa
      301 tacttgcagc caagtgatag catatatcaa atttgtattc aaaaagcctc ctcagagtat
      361 caatgtcttt tatgtcaccc tcaataaatt caacaaagtt ggggttgtca aaaaactcct
      421 ctatatttga gacctgaccg tttgaaaggt cgtctaaagc ccaaacctta tgaccgtcat
      481 ccaagagcct cttaacgacc caacgcccaa taaatcctgc accgcctgta accagtatat
      541 tcaaatcaca tcatcctctc tatcttaaat gattttgaat tacctctctg acatttgaaa
      601 tcacgtactc aatatcatcc ctatccattt taggaaataa cggaagagtt attattcttt
      661 cataaagctc ctctgcctta ggacacaaac ctttattgta gcccaattgc ctgtaataag
      721 ggtggtaata taccggaatg tagtggacat ttacccctat tcccctttcg atgagctctt
      781 caaatatctg tcttcttcca caattcagtc tttccaattt gagcttaata atgtataaat
      841 gccatgcaga atttgtgttt tcaagctggt atggtgtttc tatcgcctct gccccaccga
      901 aagcttcatt atatcttccg acaaaatctc ttctttgggc aagaaacctt tccaacttat
      961 ccaactgact gatacccagt gcagcctgaa tatcggtaat cctatagtta tagcctaagc
     1021 attgctgttc ataataccat gggccttcat cttttaataa aacatccttg tcccttgtta
     1081 taccatgggt cctgaaaagc gtgagctttt tatatagttc ttcatcattt gtcgtaattg
     1141 ctccgccttc accagttgtg atatttttta ccgggtgaaa actaaaggtg gtcatatcac
     1201 ctatgctccc gatttttctt cctttatatt ctgccccaag ggcatgggct ccgtcttcaa
     1261 ttattgtgag attatattct tgtgctattt tttgtatttc atccatatca caaggctgac
     1321 cggtaaagtg gaccggtatg attgccttag ttttttcatt tatcttagtc cttatttcgt
     1381 ccgggtcaat attgtaggtt tcagaattaa tatcggcaaa tatgggctta gcccccagat
     1441 acagcacaca attggccgaa gcggcaaagg taatagggga tgtaattacc tcatcgtcct
     1501 cttttatccc ggctgcaaag caagcagcat gaagtgccgc agtcccgttt gaaacggcaa
     1561 cggcatactt ggctccggtg cactgtgcca acttatcttc aaattccttt attttaggtc
     1621 cggtggttaa ataatcgcct cttaatactt cggtgacagc tttaatatca tcctcatcaa
     1681 tccactgccg cccgtaaggt atgaatttct tttccaacct cttatccata tgtatttctc
     1741 cctgtcaaaa taaaattatc attcctcaat aatttatttc aagcccaaaa gcagaaattt
     1801 caaacgtgag cattattaaa ggcgtcttcc aatatactga aggaaaacac ctctatatcc
     1861 aagcccttgg atatgccttt gatcaaaact aatccctgaa tcggtcacca gtttatagtt
     1921 gttactcata taaaagtcaa tcttatcatt aaatattaca gagtctgtta aagggcaatc
     1981 cggcgttacg cgtactacct catcaa
//
LOCUS       DAOOJC010000436         1996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_45075_length_1996_cov_4.138073, whole genome shotgun sequence.
ACCESSION   DAOOJC010000436 DAOOJC010000000
VERSION     DAOOJC010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45075_length_1996_cov_4.138073"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1951
                     /locus_tag="PLA01_09955"
     CDS             <1..1951
                     /locus_tag="PLA01_09955"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD01655.1"
                     /translation="KKITVLALCIMLLVGCNDNLPSDNKSITSTPDKVQNTAEVSKSE
                     EEVLLENFMEDTSVPQDVRNDLIEAMHGNTTKQDSISYEDFNFKYEVFSSIDGDKEIS
                     KAECYFNVAFLYFNGDENSKFTVDKEKALKWWKLSSDNGFYLGAVYAGDILIEGDGMP
                     RDYSKAFDFYQKAHELSENGIVYERLGDCYYFGYGTQEDKNKAYECYLNSAFGGDSQG
                     LYKLLKMTDEGYGNVDKLLLSKAASSIDYDSGYFEVAYGDIDSYKMRDDKKAAVERLT
                     EYWDKETDPAVKGMKASNPSNKWFSSDYLNEIKKTVYSYSYYGFVEEKGVIPNLTLEE
                     VNSKIKDVESEENPLYYTLKASWGHRGFYEYDFTGDGKTELFFNVNSGAGGAFMADGI
                     VILNLNEDGKYEYLTDNYGYALRDGVRLVQYGGKYYFLANPFSDFGDAPYDMDAFIFD
                     EKGDWHSVSLICKDYKAKKVVSSKDDDYISKHKLEDFLETAEKEAESAIMATREHRVY
                     KQERLKEINFSKEKEQLENNYLSGESGVDGYFEADINNDGKMEFIKIGHLISDEGKYY
                     YDINCFDVFSDKEDMENNAVPINKTDISNFDYFGLLSIGNLYGGYLPISSKLVQFWTQ
                     IYGNKTYCMAVTKQEKLYTLQVYII"
BASE COUNT          678 a          299 c          439 g          580 t
ORIGIN      
        1 gaagaaaatt actgttttag ctttatgtat aatgctgctg gttggatgta atgataattt
       61 accgtcggat aataaatcaa ttacctcaac acctgataag gtacaaaaca ctgctgaggt
      121 gtcaaaaagt gaagaggaag ttcttcttga aaatttcatg gaagatacct ccgttccaca
      181 agatgtaaga aatgatctga ttgaggctat gcacggaaat acaaccaaac aagacagtat
      241 ttcgtatgag gatttcaatt tcaaatatga agtctttagc agtatagacg gcgataagga
      301 aatttcaaaa gccgagtgtt attttaatgt tgcattcttg tacttcaatg gtgatgagaa
      361 ttcaaaattt acggttgata aagaaaaggc tcttaaatgg tggaagcttt cttccgataa
      421 cggcttttat ctgggtgctg tctatgcagg tgatatactt atagaagggg atggtatgcc
      481 ccgggattat tccaaggctt ttgattttta tcaaaaagct catgagctca gtgaaaacgg
      541 tattgtatat gaaaggcttg gcgactgcta ttactttgga tacggaactc aagaggacaa
      601 aaataaggca tatgaatgct atttaaacag tgcttttggt ggggattctc aaggactcta
      661 caaactactc aagatgacag atgaaggtta tggaaatgtc gataaattgc tgctttcaaa
      721 ggcagccagc agcattgatt acgacagtgg atattttgaa gttgcatatg gcgatattga
      781 cagctataaa atgagagacg ataaaaaggc ggcggttgaa cgtttgactg aatattggga
      841 taaggaaaca gaccctgcag ttaaaggaat gaaagcctcg aatccatcga acaaatggtt
      901 ttcgtctgat tatctcaacg aaatcaaaaa aaccgtgtat tcttattcat attatggatt
      961 tgttgaagag aaaggtgtta tacccaacct aactttagaa gaagtcaatt ctaaaataaa
     1021 ggatgtggaa agtgaagaaa atcctttgta ttacaccctg aaagcttcat ggggacaccg
     1081 gggattctat gaatatgatt ttacagggga cggcaaaact gaactattct ttaacgttaa
     1141 ctccggtgca ggaggtgcat ttatggctga cggaattgtt atactcaact tgaatgaaga
     1201 cggaaaatat gaatatttaa ctgataatta cggctatgca cttcgtgacg gggtccggct
     1261 tgttcagtat ggcggcaaat attattttct tgcgaatcct ttttccgatt tcggtgatgc
     1321 accatacgac atggatgctt ttatttttga tgagaagggt gattggcaca gtgtcagttt
     1381 aatttgcaag gactataagg caaaaaaagt tgtaagctct aaggatgatg actatatatc
     1441 aaagcacaag cttgaggact ttttggagac tgccgaaaaa gaggcagagt cagctatcat
     1501 ggccacaagg gagcataggg tatataagca agaaaggcta aaggaaatta atttttctaa
     1561 agaaaaggaa cagcttgaaa ataattatct ttcaggagaa agtggagttg acggatattt
     1621 cgaggcagac attaacaatg acggcaaaat ggagtttatt aaaattggcc atttgatcag
     1681 cgacgaagga aagtactatt acgatatcaa ctgttttgat gtgttttcgg ataaagaaga
     1741 tatggagaac aatgctgttc ctataaataa aacggacata tccaactttg actactttgg
     1801 actattgtcc attggcaatc tatatggtgg ttacttaccc atcagctcaa aacttgttca
     1861 attttggact caaatatatg gcaataaaac ctattgcatg gctgtcacaa aacaagaaaa
     1921 gctttataca ctccaggtat atataattta ggaatttgca aaatgactta ataatttaga
     1981 atgtaaagaa atgtaa
//
LOCUS       DAOOJC010000437         1996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_45078_length_1996_cov_3.680577, whole genome shotgun sequence.
ACCESSION   DAOOJC010000437 DAOOJC010000000
VERSION     DAOOJC010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45078_length_1996_cov_3.680577"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1555)
                     /locus_tag="PLA01_09960"
     CDS             complement(<1..1555)
                     /locus_tag="PLA01_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003521254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPD01656.1"
                     /translation="MKVRNVQKKKFSKKKSTIGKYNNEIIGIIILAVSIMALLSFILE
                     NSIGVFGVFIKSFIMGLVGLPGYIIPPLLIVYSVLVIFKKNTHKVNMKAIYLLGLLLI
                     MTAMLQTFYYNEQDYVNMGIAGCLKKFYSDGIAMHGGGILGGIISIPFLLVFKNLGTI
                     IILTTIAVIDIILITNVSIAGIFLRLKDDIVGIVSKIQARLKISSNEIKEKIIEEGED
                     DPEAEIDIVMNGKKLPKVVNLKEKKDEKANEQHNVGSLGVNTNNNEVVAQSSDSVVKD
                     LKKKNTEGSNVEAMTDSVNREVSSMPRLDFEYVFPHTGLLDENRQSTKNELDYRSAAI
                     RGAKKLKETLKSFGVDAKVVNVSMGPAVTRYELQPSPGVKVSKIVNLSDDISLNLAAS
                     GVRIEAPIPGKAAIGIEVPNKEMVPVFLKEVLDSKEFKENESKLAFALGKDISGKNMV
                     ADIAKMPHLLVAGATGSGKSVCINSIIISLLFNASPNDVKLLMVDPKVVELGIYNGIP
                     HLLIPVVTDP"
     gene            complement(1817..>1996)
                     /locus_tag="PLA01_09965"
     CDS             complement(1817..>1996)
                     /locus_tag="PLA01_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlzJ-like family protein"
                     /protein_id="HPD01657.1"
                     /translation="EWADQNNLELNYLGEKVVVVPLSNNRYRIERVISTSPKAYLNPK
                     LMPGSIILGNELSFR"
BASE COUNT          565 a          490 c          302 g          639 t
ORIGIN      
        1 tcggatcggt taccaccggt atcaaaaggt gcggtatacc attatatata cccagttcaa
       61 ccaccttggg atccaccatc aaaagcttga catcattggg tgacgcatta aacaaaaggc
      121 ttatgataat gctgttaatg caaacgcttt ttcccgaacc tgttgcaccg gccacaagta
      181 aatgaggcat ttttgctata tccgccacca tattcttgcc cgatatatcc tttcccagtg
      241 caaaggcaag cttggattca ttttctttaa attctttaga atccaaaacc tcctttaaaa
      301 aaaccggtac catctccttg ttgggaactt caatcccaat cgccgccttg ccgggtattg
      361 gagcctctat cctaaccccc gatgccgcaa gatttaatga tatgtcatca gaaaggttca
      421 caattttgct aaccttaacc cccggactgg gctggagctc ataccgggta actgccggac
      481 ccatacttac atttacaacc ttggcatcca ccccaaagct tttcaaagtc tctttaagct
      541 tcttcgctcc ccttatggca gcactcctgt aatccaactc gttttttgtg ctctgcctgt
      601 tttcatccaa tagtccggtg tggggaaaaa catactcaaa atccaacctg ggcattgagg
      661 aaacttcccg gttgacactg tcggtcattg cttccacatt actgccttcc gtatttttct
      721 tctttaaatc ctttacaaca gaatcagagg attgtgctac aacttcatta ttattcgtat
      781 tcacacctaa agagcccaca ttgtgctgtt cattagcctt ctcatccttt ttttctttca
      841 ggttcacaac cttggggagt ttttttccgt tcattacaat atctatttca gcctcagggt
      901 catcctcgcc ctcttcaata attttttctt ttatctcatt gctgctaatc ttcagtctgg
      961 cttgaatttt actgacaatc ccgacaatat catctttaag ccttaaaaat atcccggcta
     1021 ttgagacatt tgtaatcaga ataatatcaa ttaccgcaat agtcgtaaga ataattattg
     1081 tacccagatt tttgaacaca agtaaaaagg gtatgcttat tattccgccc agaatacctc
     1141 cgccatgcat ggcaataccg tcactgtaaa acttcttcag gcatcccgca atacccatat
     1201 ttacataatc ctgttcattg taatagaatg tctgcagcat tgcagtcatg atcagaagca
     1261 aacccaaaag gtaaattgct ttcatgttca ccttatgagt gtttttctta aatatcacaa
     1321 gcaccgaata gacaattaac aaaggaggaa ttatgtatcc cggtaaaccc acaagcccca
     1381 taataaagct ttttataaat acaccaaata caccgataga gttttcaaga ataaagctca
     1441 atagagccat aatactcact gccagtatta tgatacctat aatttcattg ttgtattttc
     1501 cgatagtgct tttctttttg gaaaacttct tcttctgtac atttcttact ttcaatttta
     1561 cacccccaca taccgccctc ataaaccaca gcttatacaa tatatcaaac aggttgtgtt
     1621 tagtatttta ttgatatttt atttatccgc tacttaataa atataacata tcacagcggc
     1681 ggctgcaatg gattccagat tcttacgact aataattccc ttatataacc tatcaatccc
     1741 tttccaatgt tattggaaat tcagcgaaac ctctgcttca ttcttcgata gatactgccg
     1801 ctccaaatat gccgttttag cggaaggaca actcgttccc caggattatg cttccaggca
     1861 tgagctttgg attcaaataa gcctttggtg aagtgctgat aaccctttca attctatatc
     1921 tgttgttgga aagaggcact accacaacct tttcccccaa ataattcaac tcaagattat
     1981 tttgatcagc ccattc
//
LOCUS       DAOOJC010000438         1987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_45377_length_1987_cov_3.824534, whole genome shotgun sequence.
ACCESSION   DAOOJC010000438 DAOOJC010000000
VERSION     DAOOJC010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45377_length_1987_cov_3.824534"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..502
                     /locus_tag="PLA01_09970"
     CDS             2..502
                     /locus_tag="PLA01_09970"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HPD01658.1"
                     /translation="MILTAIALASDIRHYKIRNKLTFTFMLIGLVTGFVLDGTDGLFD
                     SFLGIAAPFVILILLYALRMLGAGDIKLFCAIGAVMGFGEGIQIMAYSFLAGGVIAAI
                     IIIAGKNAKRRFLYMFNYLKCTFLSKSIQPYTDFEQKDDKAKFKMAYAIGCGVIIFIA
                     VDILLK"
     gene            662..1081
                     /locus_tag="PLA01_09975"
     CDS             662..1081
                     /locus_tag="PLA01_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPD01659.1"
                     /translation="MKRLADDILAKIGCKNTKPRKTVIEVLESTDNPLSAEDIFLRIK
                     ELGESVNLSTVYRTLDLMENKSLVSKTVMSDGKARYELTVDGHRHHLVCTNCHKSVPI
                     DFCPLEKLEKDIGEKNDFDITGHKLEIYGLCPKCKKE"
     gene            1373..>1987
                     /locus_tag="PLA01_09980"
     CDS             1373..>1987
                     /locus_tag="PLA01_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPD01660.1"
                     /translation="MKKNISMKFYLLGIVTIMLIMLLISAGCSGNLDADSQGSDDRIS
                     VCTSFYTMYDFAGKIGGDRIRLTNIIPAGTEPHHWEPSPKDILAIEKADVFIYNGAGM
                     EPWVEKVLGSIRNKDIEVVETSEGIELLKGEHSHDEEQEGGEENIEAEGHDSGEYDPH
                     VWLDPLMAKKQMEAIKDALVKVDPENADYYQKNFDDNANKLDELD"
BASE COUNT          622 a          295 c          463 g          607 t
ORIGIN      
        1 tataattctt acagcaattg cacttgcaag tgacatcagg cattataaga tcagaaacaa
       61 gctcacattt acgtttatgc ttattggtct tgtgaccgga tttgtacttg atggaacgga
      121 tggacttttc gattcttttc ttggtattgc cgcccctttt gtcattctaa tccttttata
      181 cgcattacga atgctgggtg cgggagatat taagcttttt tgtgcaattg gtgcagttat
      241 gggatttgga gaagggattc aaattatggc atactcgttt cttgcaggag gagtcattgc
      301 ggcgattatt attatagccg gtaaaaatgc caaaagaaga tttttatata tgttcaacta
      361 tctgaaatgt acatttcttt caaagagcat acagccctat acggattttg agcagaagga
      421 tgataaagca aaatttaaaa tggcatatgc aatagggtgc ggggtcataa tttttatagc
      481 ggtggatata ttattgaaat aatggtttaa ttgaagtaaa ataatcatat taaatacttt
      541 ggttatatct ttttttaaaa ttgtgcattt ttttcacggt tttttagcca aagtatgtat
      601 tagtgctggt gaatgcatct ttgctattag tttaatgtga aatttaaggg atgagtgtct
      661 tatgaaaagg ttggcagatg atatactggc taaaatcggc tgcaagaata caaaacccag
      721 aaagacggtt atagaagtgc tggaaagcac cgataatcct ctttcggcgg aagatatatt
      781 tttgcgtata aaggaacttg gtgaatctgt caacctgtct acggtttata ggacccttga
      841 tcttatggaa aacaaatctc ttgtcagcaa aaccgttatg agcgacggca aggcaaggta
      901 tgagctgact gtggacgggc atcggcatca tctggtctgt actaattgtc acaaatcggt
      961 tccgatagat ttttgtccgt tggagaaact ggagaaggat ataggcgaaa aaaatgattt
     1021 tgatataacg gggcataaat tggagatata tggactatgc cccaagtgca agaaagaata
     1081 aaccggtgag ttaaaaggag ctgtcttcta caatgcttaa ggcagttctt ttttattagt
     1141 gggatttcac gaagtgaaat gaccaaaaag cgatgggggc tcgatgcccc cggagctttt
     1201 attgccttac gaagtgcatt tctttattct aaagactatt gactaaagag caataatcaa
     1261 tatataattg ttttaaatgc aaatgatttg catttgaagt aaagtgttta atcgaatact
     1321 tcgatggata taattggctg taagtaacat tttacagaag ggatgtttta taatgaaaaa
     1381 aaatatttcg atgaaatttt atttattggg tattgttact ataatgctga taatgctttt
     1441 aatatccgca ggatgttctg gcaatcttga tgctgacagt cagggcagcg atgacagaat
     1501 cagtgtgtgt acaagttttt ataccatgta tgattttgca ggaaaaatcg gcggtgacag
     1561 aataaggctc accaatatta ttcctgcagg taccgagccg caccactggg aaccgtctcc
     1621 aaaggatatt cttgcgattg aaaaggcgga tgtttttata tataatggtg caggcatgga
     1681 gccttgggtg gaaaaggtac tcggttccat cagaaataaa gacattgagg ttgtagaaac
     1741 atcagaggga atagagcttt tgaaagggga acattcccat gatgaagagc aggaaggcgg
     1801 ggaagaaaac atagaggcag aaggtcatga ttctggggaa tatgacccgc atgtctggct
     1861 ggatcccctt atggccaaga agcagatgga agcaataaag gatgcattgg taaaggttga
     1921 ccctgaaaac gctgattatt atcaaaagaa ctttgacgat aatgccaata aacttgatga
     1981 actggat
//
LOCUS       DAOOJC010000439         1983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_45511_length_1983_cov_3.391598, whole genome shotgun sequence.
ACCESSION   DAOOJC010000439 DAOOJC010000000
VERSION     DAOOJC010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45511_length_1983_cov_3.391598"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1249)
                     /locus_tag="PLA01_09985"
     CDS             complement(32..1249)
                     /locus_tag="PLA01_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPD01661.1"
                     /translation="MKLKHYVYKVKTESGRIIAGETKIDSEDALRSLLQEKGYTPIEI
                     TEKNAFTDVSQISFFKPKVKLKDLAIFFRQFAIILEAGVPIAGALDVLREQTTNRTLR
                     DVLNDIYDDIQRGLSLSSAMKQHERIFPEITMHMIEAGEVSGQLDQVFVRLAGQYEKD
                     YKLNRSIRSAMLYPIIVCVISIAVIFVLMVGVLPVFSDTLGSLNVEMPLITKIVMGSS
                     RFFVKWWWAIIGAIVGLVLFLVSYLRTYNGKRLISSIALKLPVVSGLVQNITTARLAR
                     TLGTLISSGVLLIQAMQITQKVLGNVVISERISGVIDEIKMGKGLTQPLKNLRYFPPM
                     LISMMRVGEESGNLDYSLEKSADFYDEEVETSIQRLTTLLQPLIILILAGIVTVIVLS
                     VLLPMYSIYENIQ"
     gene            complement(1646..>1983)
                     /locus_tag="PLA01_09990"
     CDS             complement(1646..>1983)
                     /locus_tag="PLA01_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HPD01662.1"
                     /translation="IDVFPPHQQAQVRTQLSTVLMGIISQQLLRRADNKGRVLATETM
                     VWTPAIANLIRESRTHQINTCIQTGSQFGMYTMDSCLADLYKQELIDYETACQYSVDM
                     DNLRKLLSK"
BASE COUNT          646 a          422 c          295 g          620 t
ORIGIN      
        1 tatgtttaga acagggttac tgaaaataaa cttattgtat attctcatat atactgtaca
       61 tcggaagcag aacacttaaa acaataaccg taacaatacc tgccaatatt aaaataatca
      121 atggctgtaa caatgtcgtc aatctctgta tagaggtttc aacttcttca tcgtaaaaat
      181 cagccgactt ctcaagggaa taatctagat taccggattc ttcccctact ctcatcattg
      241 aaatgagcat tggcggaaag tacctgagat tctttaaggg ttgcgtcaaa ccttttccca
      301 tctttatttc atcaataaca ccacttatcc gctccgatat aacaacatta ccaagaacct
      361 tttgagtaat ctgcattgct tgtatgagga gtactccgct tgatatgaga gtccccagtg
      421 tacgggcaag cctggcggtt gtaatatttt gtaccaatcc actaaccaca ggcaacttta
      481 atgctataga agaaataagc cttttaccgt tataagttct aagataagaa acaagaaaaa
      541 gtacaagtcc aactatagct ccaataattg cccaccacca tttgacaaaa aagcggctgc
      601 tacccataac tatcttcgtt atcaatggca tttctacatt taaactcccc agtgtatctg
      661 aaaaaacagg taatactcca accataagaa caaatataac cgctatggaa ataacacata
      721 caataatcgg ataaagcata gcacttctga tactgcggtt taatttatag tctttttcat
      781 actggccggc aagtctcaca aaaacttggt caagctgacc gcttacctca ccggcttcaa
      841 tcatatgcat agttatctct ggaaaaatcc tttcatgctg cttcattgca ctggaaagag
      901 acaatcctct ctgtatatca tcatatatat catttaaaac gtccctcaat gtcctgttgg
      961 tcgtctgttc tcttaataca tccaatgctc cggcaatcgg aacaccggcc tccaatatta
     1021 ttgcaaactg tctgaagaat attgctaaat ccttaagctt tactttaggc ttaaaaaagc
     1081 tgatctgact tacatcagta aaagcatttt tttccgttat ctcaataggg gtatacccct
     1141 tttcctgcaa aaggctccta agggcatctt cgctatctat tttggtctca ccggctataa
     1201 tcctaccgga ctcggtctta accttataca cataatgctt cagcttcatc tacactaacc
     1261 cccaaatgat attgaacact tatcctgttt ttttatttaa taaagaaacc attaaattag
     1321 caataacact aacagtaatt attgtgtagt cctttgaatg tggtttgatg agatattaca
     1381 gatatattaa cataatagaa caaatcaaaa ctcattgttt tcaggcttat tttagattcc
     1441 tataatattt taccggtcga aaaagttgaa actaaaatca gatatgtaat caatcattcc
     1501 aacctcccaa attaattcac accaatccaa caaaataata ttatcaaaaa ttatctttaa
     1561 gtcattgtaa tatataacat tcttatcttt aattactact taaatatatt atattacact
     1621 ttcccaataa aagctagtat tttttttatt ttgatagtaa ttttctcaaa ttatccatgt
     1681 cgacagagta ctggcaagca gtctcatagt caattaactc ctgcttgtat aaatctgcaa
     1741 gacaactgtc catagtatac ataccaaact gggaaccggt ctggatacat gtatttattt
     1801 ggtgggtacg gctctcacgt ataaggtttg caatagcagg agtccatacc atggtttcag
     1861 tagcaagaac acgtccctta ttgtcagccc gccttaaaag ctgttgagat ataataccca
     1921 taaggactgt tgaaagctgg gttctgacct gtgcctgctg atgcggtgga aaaacgtcaa
     1981 tta
//
LOCUS       DAOOJC010000440         1976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_45733_length_1976_cov_3.864133, whole genome shotgun sequence.
ACCESSION   DAOOJC010000440 DAOOJC010000000
VERSION     DAOOJC010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45733_length_1976_cov_3.864133"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1442)
                     /gene="rpoN"
                     /locus_tag="PLA01_09995"
     CDS             complement(93..1442)
                     /gene="rpoN"
                     /locus_tag="PLA01_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPD01663.1"
                     /translation="MDFYFDLSLIQSQKIILTPQLRQAIEILKMNSQELSEYILREME
                     TNPALEMNSPFSETQEETEELENDNYYDAGVRHDDSENRVSRVFLDTSAVGLSLKEHL
                     LLQLHALSMSEIDIEIAEYLIDNVDDNGYLCINLSEVAAFFNIPIRKVEKNLNLLQTF
                     DPPGICARNLKECLLIQLNQMEIQDRDILNIVMNHLDGLADNKLSAVAKETGLSIKRV
                     AEVLEFIKTLEPRPGREFYGLDGVKYIIPDVTVKEINDRFEVLINEESTPPITISSYY
                     KKVLKEDINIEAKKFIQSKIDSVAWLIKCIQQRKNTLIRVSEHIVNSQIEFFKKGKGY
                     IKPLTMKEVAAMLDIHESTVSRTVSGKYLQCSWGVFDMKYFFPPRVLAASGKEITGNS
                     IKKKIQGIIDSEDKKHPLSDSQIVDILKKEGLEISRRTVAKYRCDLKIPSSSKRKKY"
     gene            complement(1509..>1976)
                     /locus_tag="PLA01_10000"
     CDS             complement(1509..>1976)
                     /locus_tag="PLA01_10000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPD01664.1"
                     /translation="ESRGSISAVVISSILFGIFHFDVKNLTGPIFLGMLIGYYVIRTD
                     SIFAGMLAHFANNLLSVLVQYFSRSWAGIGDSAMITEDQFIAVATFGIICFVVMAFLL
                     FCFHKTTRGRCELKPSRTSIIKDITSVVRHWPVFMSLLLYVLYSVVFLINQVL"
BASE COUNT          597 a          432 c          314 g          633 t
ORIGIN      
        1 atttagcgga attaattaac taattagtgt gtttacccac acaaaatagc gaagcaccta
       61 aattataagg ggaaatatta aatggacagt tattaatact tctttctttt tgacgaagag
      121 gggattttta aatcacaacg gtactttgcc acagtacggc gggatatttc aagtccttct
      181 ttctttaaga tatcaactat ttgactatcg ctcaaaggat gcttcttatc ctcagaatct
      241 attatacctt gtatcttttt cttaatactg ttgcctgtga tttctttccc tgatgcagct
      301 aaaacccttg gaggaaagaa atatttcata tcaaaaactc cccatgagca ttgcaaatat
      361 ttaccgctga cggttctgct cacggtggat tcatggatat caagcatagc agcaacctct
      421 ttcattgtaa gaggctttat atagcccttt ccctttttaa aaaactcaat ctgactgttc
      481 actatatgct ccgataccct gataagtgtg ttctttcgtt gctgtatgca ctttatcaac
      541 caggctacac tgtctatttt tgattgaata aatttttttg cctcgatatt tatatcctct
      601 ttcaatacct tcttatagta actgcttatg gttataggag gtgtggactc ttcattaata
      661 agcacctcaa acctgtcgtt aatttccttt acagtaacat cgggaataat gtatttgaca
      721 ccgtcaagtc cataaaattc cctgccgggt cttggctcca aggttttaat aaactccagt
      781 acctcggcca ctctttttat actgaggcct gtttcttttg cgactgcgga taatttatta
      841 tcagccagcc catccaagtg gttcatgact atgtttagta tatccctgtc ctgtatttcc
      901 atctgattca actgtatcaa aaggcattcc ttcaaatttc ttgcacaaat acccggtggg
      961 tcaaaagttt gtaacagatt taggtttttt tcaaccttgc ggataggaat gttaaaaaaa
     1021 gcagccacct cggaaagatt tatgcaaagg tacccattgt catcaacatt gtctatcaaa
     1081 tactcagcta tttctatatc tatttcactc atggacaggg catgaagctg aagaagcagg
     1141 tgttctttca aagacaatcc gacagcggaa gtatctaaaa acactctgct gacacggttc
     1201 tctgaatcgt catgcctaac ccctgcatca taataattat cattttccag ctcttcagtc
     1261 tcctcttgag tctctgagaa aggagagttc atctccaaag caggatttgt ctccatttcc
     1321 ctaaggatat attccgacaa ttcctgtgaa ttcattttaa gtatttcaat cgcttgcctt
     1381 aactggggtg taagtattat tttctgcgat tggataagac ttaaatcaaa ataaaagtcc
     1441 aatttactcg ccatccttta aaattattca agccgatacc aatacaaaag ttttactaat
     1501 ctctatctct acaatacctg gtttatcaga aaaaccaccg aatacaaaac atacaataac
     1561 aggctcataa ataccggcca atgccgcacc actgatgtaa tatcttttat tatgcttgtt
     1621 cttgagggct ttaattcaca tctgcccctt gttgttttat gaaaacaaaa gagcaaaaac
     1681 gccataacaa caaaacagat tattccaaaa gtcgccactg ctataaactg atcctcggtt
     1741 atcattgcac tatccccaat acctgcccaa ctacgggaaa aatactgtac cagcactgac
     1801 agcaagttgt ttgcaaagtg tgccaacata ccggcaaata tggaatcggt tctgattaca
     1861 taatatccta taagcatccc caaaaagata ggtcctgtaa ggtttttaac atcaaaatgg
     1921 aaaataccga acagtatgga tgaaatcacc acagcactta tagaacctct gctttc
//
LOCUS       DAOOJC010000441         1971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_45918_length_1971_cov_3.742693, whole genome shotgun sequence.
ACCESSION   DAOOJC010000441 DAOOJC010000000
VERSION     DAOOJC010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45918_length_1971_cov_3.742693"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLA01_10005"
     CDS             <1..434
                     /locus_tag="PLA01_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPD01665.1"
                     /translation="GICGFGMAADITFKSIDSNAEHCSSIKNMFIERLKDEVEDAVVI
                     SPENSSPYILNVSFAGIRAEVLLHHLEARNIFVSTGSACSSRKHVHSHVLKAMGIKTE
                     LIEGAIRLSFSSFNTQEDIIETVEAIKDILPKIRIKRGGRK"
     gene            431..1609
                     /gene="thiI"
                     /locus_tag="PLA01_10010"
     CDS             431..1609
                     /gene="thiI"
                     /locus_tag="PLA01_10010"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HPD01666.1"
                     /translation="MKKVILIRYGEILLKGLNRSMFENKLISNIKRSVHKLGKVTVRK
                     SQARIYIEPDEDSYNFDEAMELISKVFGIVSVSPVWKIDSDYNTIKEYSVKMVKDLVE
                     RKGHSTFKVETKRGNKQFPMDSPEISKDLGAHILKSVPGLSVDVKKPHFILYVEVRDF
                     TYIYSEIIPAVCGMPIGTNGKALLLLSGGIDSPAAGWMIAKRGVEIDAIHYYSYPYTS
                     ERAKEKVIELTKILASYCYSINLHIVPFTDLQMEIYEKSPHEELTIIMRRVMMQIAEK
                     IAEKTGSLALVTGESVGQVASQTIHSLAVTNAAVSMPVFRPLIGMDKNEVVNIAKKIG
                     TYETSILPYEDCCTVFVAKHPVTRPKLDRIMLSESKLDLDALIEKAIENTEVITVTAE
                     "
     gene            1718..>1971
                     /locus_tag="PLA01_10015"
     CDS             1718..>1971
                     /locus_tag="PLA01_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanomycin resistance protein VanB"
                     /protein_id="HPD01667.1"
                     /translation="MFVAIISKHKIKITLIISVLLLTITFFLARIIIILNEDTFYKGV
                     MVEGIDVSGLGKSQAKELVEKKINRIIAENSILLHYEGQT"
BASE COUNT          653 a          306 c          466 g          546 t
ORIGIN      
        1 cgggaatttg cggttttggt atggcagccg atattacatt taagagcatc gacagtaacg
       61 cagaacactg cagctctatc aaaaacatgt ttattgaaag gctgaaagat gaagttgagg
      121 atgctgttgt catatccccg gaaaactcgt ccccttatat tttgaatgtt tcgtttgccg
      181 gtattcgtgc agaggtactt ttacaccatt tggaggcaag gaacatattt gtgtccacag
      241 gatccgcctg ctcttcaaga aagcatgttc acagtcatgt gctcaaggca atgggaatca
      301 aaaccgagct gattgaagga gcaataaggt tgagtttctc atcctttaac acacaggagg
      361 atataataga aactgtggaa gctataaaag atatattacc gaagatcaga attaagcgtg
      421 gaggaagaaa atgaaaaagg ttattttgat aagatacggg gaaatacttc taaaagggct
      481 gaacaggtct atgtttgaaa ataagcttat aagcaacatt aaaaggtctg ttcataaact
      541 gggcaaggta actgtgagaa agtctcaggc aagaatatac attgagccgg atgaagacag
      601 ctataatttt gatgaggcaa tggagcttat ttcaaaggtt tttggaattg tttcggtgag
      661 tccggtatgg aagatagatt ccgattataa taccattaag gaatattccg ttaaaatggt
      721 gaaggacttg gtggaaagaa aggggcattc aacctttaag gtggagacaa aaagaggaaa
      781 taagcagttt cccatggatt cccctgaaat aagcaaagac ttgggagcac acatacttaa
      841 aagcgttccc ggattgagtg tggatgttaa gaaacctcat tttattctgt atgtggaggt
      901 gcgggatttt acctatattt attcggaaat aattcctgcg gtttgcggta tgccaatagg
      961 gaccaatggc aaggcactgc ttttgttgtc gggaggtatt gacagccctg cggcaggttg
     1021 gatgatagct aaaagaggtg ttgagataga tgcgattcat tattatagct atccctatac
     1081 cagtgaaagg gctaaagaga aggttattga gctgacgaaa attcttgctt cctattgtta
     1141 tagtataaac cttcacatcg ttccctttac cgaccttcag atggagattt acgagaagtc
     1201 cccccatgaa gagcttacca taattatgag aagggtaatg atgcaaatag ccgaaaagat
     1261 agccgaaaaa actggttcat tggcattggt taccggtgaa agcgtgggac aggtggcaag
     1321 ccagacaata catagtctcg cggtaacaaa tgcagcggtc agtatgcctg ttttcagacc
     1381 tttgattgga atggataaga atgaggtggt gaatattgca aagaaaatag gtacctatga
     1441 gacatctatt cttccttatg aggactgctg tacggttttt gttgccaaac accctgtgac
     1501 aaggccaaaa cttgacagga taatgctttc ggaaagcaaa ttggatcttg acgcacttat
     1561 agagaaggcg atagaaaaca ctgaagtgat aacggtaacg gcagaataac aattgaatat
     1621 aaaaataatg gatgtatgta atattatatt aaattgcaac aatatctata tagtagataa
     1681 gctttgaagc atttacttac gtaaagaagg tatggatatg tttgttgcaa taatttcaaa
     1741 acataaaatt aagataacat taattataag tgttttgctt ttgacaataa cgttctttct
     1801 ggcacggatt attattatat tgaatgaaga caccttttac aagggcgtta tggtggaagg
     1861 tatcgatgta tcggggcttg gcaaaagcca ggcaaaagaa ctggtagaaa aaaagataaa
     1921 caggataatt gctgaaaaca gtattctttt gcactatgaa ggccagacat g
//
LOCUS       DAOOJC010000442         1965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_46115_length_1965_cov_4.670157, whole genome shotgun sequence.
ACCESSION   DAOOJC010000442 DAOOJC010000000
VERSION     DAOOJC010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46115_length_1965_cov_4.670157"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..>1965)
                     /gene="glgP"
                     /locus_tag="PLA01_10020"
     CDS             complement(209..>1965)
                     /gene="glgP"
                     /locus_tag="PLA01_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPD01668.1"
                     /translation="VYHMNEGHSAFLGLELIRKLVQNKSLPFKQAKEIISSSVIFTTH
                     TPVPAGNDVFPLEMIDRYFGNYWPSLGISKHEFLDLGLKIGDHQNFNMTVLALTLSGQ
                     KNGVSELHGAVSRNIFKNVWSGIPEDEVPIGHITNGIHTLTWLSPSIKLLYDKYLDKD
                     WEDRLYEKETWDKVDDIPDEDLWKTHCVLKTKMIAFIREKLKQQRMSNGDSIERIREV
                     DTLLDSNALTIGFARRFATYKRANLIFRDLARIQKLLNDPQRPMQIIFAGKAHPADGP
                     AHEIIKHINDIARQEGFIGKVILIENYNMTLARNMVQGVDIWLNNPRRPLEASGTSGQ
                     KVCINGIINFSILDGWWCEGYNGKNGWVIGDDTFYDNEYHQDNADSESIYNTLEKLII
                     PAFFERNEKGIPEKWVAVMKESIKSNTYRYSTHRMVQDYTNKYYITSMNRHDKIMSSN
                     YQFATSLSDWKQRVEQLWPQVQILADKTANHLKERSFISGETIPINAYVSLGGLEPSS
                     VKVQVYCGSIGNNNAIENAEIIDMELKEKNADGTYTYSANITLFEGGEYGYTFRVIPY
                     HPDMINPFNIGLIRWVVQ"
BASE COUNT          516 a          422 c          404 g          623 t
ORIGIN      
        1 gtacggttca acgagaggga aataattctt agcaattatt tctctactct attagaaggt
       61 gttgaatgaa ataaaagctt taagcgaggg ggtgctcgat agccttgagc ttttattaat
      121 gggatttcac gaagtgaaat gacccaaagg tgagaggact tgagtgaaaa actcaatgtt
      181 cccgaattat caatgtttac tatatatttt actgaaccac ccagcgtatt aatccaatat
      241 taaacggatt aatcatatcg gggtgataag gaatgaccct gaaagtatag ccgtactctc
      301 cgccttcaaa caatgttatg tttgcagagt aggtatacgt tccgtcagca tttttttcct
      361 ttaattccat atcgataatt tctgcatttt caatagcatt attattgcca atgctgccgc
      421 aataaacttg taccttaacg ctcgaaggct caagaccgcc caggcttaca tatgcattaa
      481 tcggaattgt ttctccggat ataaaacttc tttccttcaa atggtttgca gttttatctg
      541 caagtatctg tacctgcggc cataactgct caacacgctg tttccaatcg gaaaggctgg
      601 tggcaaactg atagttgctg gacattatct tgtcatgcct gttcatggat gtaatgtaat
      661 acttgtttgt atagtcctga accatcctgt gggtactgta cctgtaagta ttggatttta
      721 tcgattcctt cattacagcg acccattttt caggtatgcc tttctcattc ctttcaaaga
      781 aggcaggaat aataagtttc tccagtgtat tataaataga ttcactgtcg gcattgtcct
      841 gatgatactc attgtcatag aaggtatcat ctccaataac ccaaccgttt ttgccattat
      901 agccttcgca ccaccatccg tcgagtatgc tgaaattgat aatgccgttg atgcatacct
      961 tctgtccgct ggttccgctg gcttcaagag gtcttcttgg gttgttgagc cagatatcca
     1021 ctccctgaac catgtttcgg gcaagggtca tattgtagtt ctcgataaga attacctttc
     1081 cgataaaacc ttcctgcctt gctatatcgt taatgtgctt gatgatttca tgagcaggac
     1141 catcggcagg atgagccttt ccggcgaata ttatctgcat gggtctttgg ggatcattca
     1201 gaagtttttg gatgcgggca agatccctga aaatcaggtt tgccctctta tatgtggcaa
     1261 atctccgggc aaatccgatg gtcaatgcat tggaatcaag cagagtgtct acttctctga
     1321 ttctctcaat agaatcgccg ttggacatcc tttgctgctt cagcttttcc cgaatgaaag
     1381 ctatcatctt tgttttaagg acacagtgag ttttccataa atcctcgtcc gggatatcgt
     1441 caaccttatc ccaggtttcc ttttcgtaaa gacggtcttc ccagtcttta tcgagatact
     1501 tgtcatacag gagcttgata ctcggagaca accaggtaag ggtgtggata ccgttggtaa
     1561 tgtgtcctat cggcacttca tcctcaggga taccggacca gacatttttg aaaatattcc
     1621 tcgatacggc accgtgaagc tcactgactc catttttctg ccccgaaagt gtcagggcaa
     1681 gaactgtcat attaaagttt tggtgatctc ctattttaag tccgagatct aaaaactcat
     1741 gtttactaat acccaaagaa ggccagtagt tgccgaaata cctgtcgatc atctccaatg
     1801 ggaacacgtc atttccggcc ggtacgggtg tatgcgtcgt aaatatgaca gaggaagaaa
     1861 ttatttcttt tgcctgctta aaaggtaagc tcttgttctg taccagtttt cttataagtt
     1921 caagacccag aaaagcggag tgtccttcat tcatgtgata tacgg
//
LOCUS       DAOOJC010000443         1947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_46757_length_1947_cov_4.073467, whole genome shotgun sequence.
ACCESSION   DAOOJC010000443 DAOOJC010000000
VERSION     DAOOJC010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46757_length_1947_cov_4.073467"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..937)
                     /gene="pflA"
                     /locus_tag="PLA01_10025"
     CDS             complement(212..937)
                     /gene="pflA"
                     /locus_tag="PLA01_10025"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HPD01669.1"
                     /translation="MTIKGRIHSFESFGTLDGPGIRFVVFMQGCPLRCLYCHNRDTWD
                     AGGGEEYTPSQIIDEMKKYIDYIRFSGGGITVSGGEPTMQAEFVTEVFKLAKGMGVHT
                     ALDTNGFADIEKVEELLRYTDLVLLDIKHAVEEKHRRITGVSNEKILKFSEYVDGQGI
                     PVWIRYVLVPGYTDDEEDLKLAAQFIGRLKTVKKVEVLPYHSMGAYKWEKLGQKYELE
                     GVREPDEKEVQKAKRILCSGMDG"
     gene            complement(955..>1947)
                     /locus_tag="PLA01_10030"
     CDS             complement(955..>1947)
                     /locus_tag="PLA01_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPD01670.1"
                     /translation="IGKQMQFCGARCNLAKALLYAINGGKDEMTGDQIAPMFAAIDTE
                     YLDYDDVMKRFDMVLDWVARLYINTLNIIHYMHDKYAYEKLQMSLHDREVFRTMACGI
                     AGLSVVADSLSAIKYAKVKPIRNENNLVVDYEVEGDYPKFGNNDDRVDDIATGIVKKF
                     MDKLRKQRTYRGAMPTLSILTITSNVVYGKKTGNTPDGRKTGEPLAPGANPMHGRDIK
                     GALAVLNSISKLPYEYAQDGISYTFSIIPKALGRDEEARINNLKAMLDGYFGQGGHHI
                     NVNVFEKETLLDAMEHPEKYPQLTIRVSGYAVNFIKLTREQQLDVINRTIHEKI"
BASE COUNT          519 a          483 c          330 g          615 t
ORIGIN      
        1 ataccgcccg tatatttgct atgatatttg acaagctaaa cctgacagct tttattccgg
       61 tgaaaatcaa gcacaagtat aataaaccga agaaactatc cggtacagtg caaaataaac
      121 tttctgccga aaataaatgt gaatagattg atagtaatac ataaaacctt cgcaatgttt
      181 attccacaat ttcactcgat taaataatat gttatccatc cattccactg cacagaattc
      241 ttttagcttt ttggacctct ttctcatcag gttcccttac accttcaagc tcatacttct
      301 gtccaagctt ttcccactta tatgctccca tgctgtgata aggcaaaact tcgaccttct
      361 ttacagtctt aaggcggcca atgaattgtg ctgccagttt gaggtcctcc tcatcatcag
      421 tatatccggg aacaagtaca tatcttatcc agacaggaat cccctgccca tctacatatt
      481 ctgaaaattt taatattttt tcattggata ccccggtaat acgtctgtgt ttttcctcaa
      541 cagcatgctt tatatcgagc aaaacaagat cggtataccg caataattcc tctaccttct
      601 caatatcggc aaaaccgttg gtatccaggg ctgtatgcac gcccatgccc tttgcaagct
      661 tgaaaacctc tgttacaaat tcagcctgca tagtcggctc tccacctgaa acggtaattc
      721 ctccaccgga aaaccttata tagtctatgt attttttcat ttcgtcgatt atttgcgaag
      781 gagtatattc ctctcctccc ccggcatccc atgtatctct gttatggcaa tataaacagc
      841 gaagggggca accctgcatg aaaaccacaa accttatacc cggtccgtca agtgtcccaa
      901 aagactcaaa tgaatgtatt cttcccttaa tagtcattgc ccccaccccc gcatttatat
      961 tttctcatga atagtcctgt tgataacgtc aagctgttgt tctcttgtaa gctttataaa
     1021 gtttaccgca taacccgata cccttatggt tagctgcgga tatttttcag gatgttccat
     1081 ggcatcaaga agagtttcct tctcaaacac gttaacattt atatgatgac ctccttgacc
     1141 gaaatatccg tcaagcatag ccttaaggtt gtttatccta gcttcctcat cccttcccag
     1201 ggctttggga attattgaaa atgtatagga tataccgtcc tgagcatatt cataagggag
     1261 ctttgatata ctgtttaaaa cagccagtgc tccttttata tcccttccgt gcataggatt
     1321 tgcccccggt gccaagggtt cgcccgtttt tcttccatcc ggtgtgtttc ccgtcttttt
     1381 gccgtaaacc acatttgagg taattgtgag aatagacagt gtcggcattg ccccacgata
     1441 tgtcctctgt tttctaagct tatccataaa ttttttaact atgcctgtgg ctatatcgtc
     1501 aacacggtca tcattattac cgaacttagg gtaatctccc tccacctcat aatcgacaac
     1561 cagattgttt tcatttcgta tgggcttcac cttcgcatat tttatggcac taagggaatc
     1621 tgccacaaca gacaaccctg caataccgca ggccattgtt ctgaaaactt cccggtcgtg
     1681 aagcgacatc tgcagttttt cgtaggcata tttgtcatgc atatagtgta ttatattaag
     1741 cgtgtttatg tacagccttg caacccagtc aaggaccata tcaaaacgct tcattacatc
     1801 atcataatcg agatattcgg tatctattgc cgcaaacatc ggtgcaatct ggtcacctgt
     1861 catttcgtcc tttccgccgt taatagcata tagaagtgct ttcgcaagat tgcatcgggc
     1921 accgcagaac tgcatttgtt ttccgat
//
LOCUS       DAOOJC010000444         1939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_47047_length_1939_cov_3.389597, whole genome shotgun sequence.
ACCESSION   DAOOJC010000444 DAOOJC010000000
VERSION     DAOOJC010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47047_length_1939_cov_3.389597"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="PLA01_10035"
     CDS             complement(<1..1358)
                     /locus_tag="PLA01_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPD01671.1"
                     /translation="MLDLPDSGLSGGENGNNKSQAQNNSDNNHDDPTFNGQEPYNEYA
                     DSFEQWKERSQRRREEKKRGSLDGDYNGGDMPDNRIQGRGGNNGIISGGGGMPQDGNI
                     PTDIPNIDISGDNALPDMPFGMQGPGKTIVMGDEKRPHIAAFEVLGKPNYPFLKVMVM
                     ENYNNNRWIMAQEEPLEKFEFGVDKGRNYEDNSVKIKPIEPSKGYMPVLSGNFKMIYD
                     LSILEYQNSGTYYSEGIVEDFYEMEYEKAPSEIDLIYAETDDDYPYEISVNEYIDSIV
                     DEVTERCSSDYDVIYSVEQYLLNNYTLSNNVKNNYKNTDGITEFLKYKKVGNYLDFVS
                     AYTFLLRAAGIPCRLAMGYRLMEQVPYQVVYADQVYIYPEIKFKDYGWVPMDVFAYEP
                     FYTPPIETVTDIVFADGTARRGDEFTVKGTAKNVYGSPIDDMTVLIYVKEDKEKPCLS
                     YT"
     gene            complement(1486..>1939)
                     /locus_tag="PLA01_10040"
     CDS             complement(1486..>1939)
                     /locus_tag="PLA01_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2161 family putative PD-(D/E)XK-type
                     phosphodiesterase"
                     /protein_id="HPD01672.1"
                     /translation="LRRLEIGLLLVSFRGDVAMVEEAIEPIPFSREKSRSAANKKKGL
                     VLKEFSQRQGDYNTGGCTRKKLVTAYREKSIQIAALIKAEGPLSIKQLKDRGADSDKV
                     SQILQNNFYDWFIRVSRGVYDLRKRGKSEIESYSELLEYYTRLRCTKQ"
BASE COUNT          525 a          486 c          298 g          630 t
ORIGIN      
        1 tttgtgtagg aaagacatgg cttctccttg tcctctttaa cataaatgag gactgtcata
       61 tcatcaatgg ggcttccgta tacgttctta gcagtgccct ttactgtaaa ttcgtcacct
      121 cttcttgcag tgccgtcggc aaatactata tcggtcaccg tctctatcgg aggtgtatag
      181 aagggctcat aagcaaacac atccatcggt acccatccat aatctttaaa ttttatttca
      241 gggtaaatat atacctgatc tgcataaacc acctgatacg gaacctgctc cataagcctg
      301 taacccattg ctagcctgca gggaataccg gcagcccgca aaagaaaagt ataagcagac
      361 acgaagtcca gataatttcc tactttttta tacttcaaaa attctgtaat tccatccgta
      421 tttttataat tattcttaac attattactt aaagtatagt tattcagtaa atactgttca
      481 acagaataaa taacatcgta atcactgctg catctctccg tcacctcatc aacgattgaa
      541 tcgatatatt cattgacgga aatctcgtag gggtaatcat catccgtttc agcataaata
      601 agatctattt ctgagggagc cttctcatac tccatttcat agaaatcctc aacaatcccc
      661 tctgaataat aagtacccga gttttgatat tccaatatgc tcagatcata aatcatttta
      721 aaattgcccg acaataccgg catataaccc ttcgaaggct caatgggctt aatttttacg
      781 gagttgtcct cataatttct gcccttatca actccaaatt caaacttctc caacggttct
      841 tcctgagcca taatccaacg gttattatta taattctcca taaccattac cttcaaaaaa
      901 ggatagttcg gcttgcccaa gacctcaaaa gcagcaatat gaggccgctt ttcatcaccc
      961 atcacaatgg ttttacccgg tccttgcatc ccgaaaggca tatccggcaa tgcgttatct
     1021 cctgaaatgt ctatattggg aatatccgtg ggaatattgc catcctgcgg cataccgcct
     1081 cctccggata taatgccgtt atttcctcct cttccttgaa tccggttatc aggcatatct
     1141 ccaccgttat agtccccatc aagactacca cgtttttttt cttcacgcct tctctgtgac
     1201 ctttccttcc actgttcaaa gctgtcagca tattcattat aaggttcctg tccattaaac
     1261 gttggatcgt catggttgtt atcgctatta ttctgtgcct gggacttgtt attgccgttt
     1321 tcacccccgc taagtcctga atctggcaaa tccaacatgc ttatagcttc cgtaataagt
     1381 aatgcagata taaatacaac ggaaagaaaa attgccaaac ttattaaatt tttccgacta
     1441 tttccattca tcttttccat atacagtatc aattatatag gatttttatt gttttgtaca
     1501 tctcaatctt gtataatact ccaacaattc actgtagctc tcaatttcac tcttgcctct
     1561 ttttcttaga tcgtatacac ctctggatac tcttataaac caatcataaa agttgttctg
     1621 cagtatttgg gaaaccttat cgctgtccgc tcccctatcc tttagttgtt ttatactcaa
     1681 cggaccttcg gctttaatca gtgctgctat ttgaatcgac ttctccctgt aggccgtgac
     1741 caactttttt ctggtgcatc ccccggtatt ataatcgccc tgtctttggg aaaactcttt
     1801 taaaaccaaa ccctttttct tgttggccgc acttctgctc ttctcacggc taaagggtat
     1861 cggctctatg gcttcttcca ccatggctac gtcgcccctg aaagaaacca gcagcaaacc
     1921 gatttctaaa cgcctcaaa
//
LOCUS       DAOOJC010000445         1929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_47412_length_1929_cov_2.946638, whole genome shotgun sequence.
ACCESSION   DAOOJC010000445 DAOOJC010000000
VERSION     DAOOJC010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47412_length_1929_cov_2.946638"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PLA01_10045"
     CDS             complement(<1..921)
                     /locus_tag="PLA01_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPD01673.1"
                     /translation="MTRKLLVFILNCIIVFLFASCTNIDVGPFESRDYIMGTLVIQRI
                     YHKEADRIHGEVNERIAGIEAKMTINKPGGEINLLNDAAGEDYVVLGDDTLYVLDKAR
                     HFSDISNGVFDVTVGPLVKTWGIWSDNPRVPGKNEIDELLKLVNYKDVLIEKDNDRAM
                     LKKSRQVVDVGGIAKGYAADEAIRIYKENGVKSAFVSLGGNVAVLGGKPDGSPWRIGI
                     RNPKGPRDSYIGIVSIKDGSVVSSGEYERFFEEKGVRYHHILDPKTGFPAESDLAGVT
                     IVSDLSIDADALSTAVFVLGLEDGMRLIEGI"
     gene            complement(1085..1753)
                     /locus_tag="PLA01_10050"
     CDS             complement(1085..1753)
                     /locus_tag="PLA01_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPD01674.1"
                     /translation="MVGNIIENNMVTISGKIVSESKFSHEVYGEGFYTFTLEVSRLSD
                     SYDRIAVTVSERLVSREKLEIGQFIEIEGQFRSYNNYNNDAGSRLVLTVFAREVNFFE
                     DERKIKNPNQIYLNGFICKKPIYRTTPFGREITDILLAVNRPYNKSDYIPCICWGRNA
                     RYASGLEIGDNIKIWGRIQSREYQKKQENGEVITKMAYEVSVSKMEVLSSEKDIPSFD
                     EDDS"
BASE COUNT          534 a          511 c          272 g          612 t
ORIGIN      
        1 aatcccctca ataagcctca ttccatcttc caatcccaat acaaaaaccg ccgttgaaag
       61 agcatccgca tctattgaga ggtctgaaac gattgtaaca ccggccagat cggattcggc
      121 ggggaaaccg gtcttagggt ctaaaatatg gtggtaccga acacccttct cctcaaaaaa
      181 cctttcatat tcccccgaac tgaccacaga cccatccttt atactgacaa tgccaatata
      241 tgaatctcgc ggccccttcg gatttcgtat gccgattctc cacgggctgc cgtcgggttt
      301 gcctcccagt acggcaacat ttcctccaag gcttacaaat gcagacttca ctccgttttc
      361 tttatatatc cttatagctt catctgctgc ataccctttc gcaatccctc ccacatccac
      421 aacttgtctg ctttttttta gcattgcccg gtcattgtct ttctctatca aaacatcttt
      481 ataattaacc agcttcaaaa gctcgtcaat ttcattcttt cccggaactc tagggttgtc
      541 cgaccatatc ccccatgtct tgacaagagg ccctacggta acatcaaaca caccattgct
      601 tatatccgaa aagtgcctgg ctttatccag cacgtaaagg gtatcgtctc caagcactac
      661 ataatcctct cccgctgcat cgtttaaaag atttatttct cctcccggct tgtttattgt
      721 catcttcgcc tcaattccgg caattctttc gtttacctcc ccatgaatcc tatctgcttc
      781 cttgtggtat atcctttgaa tcactaatgt tcccattata taatcccttg actcaaaagg
      841 accaacatct atattggtgc atgaagcaaa caaaaaaaca attatacaat tcaaaataaa
      901 aaccaacagc ttcctggtca taaaatcaac tccgcacaat aaaactcaat cccaatgtat
      961 aggcttcgga tcatcgattc ttcccgcttt ttgctcattt tacccaagcc ctgcaacata
     1021 cttaaaggtg gtctgcccgg cagccacctt ttataatata ctaacaagct tattctatac
     1081 aatcctaaga atcatcctca tcgaatgagg gtatatcttt ttcagagctt agaacctcca
     1141 ttttggaaac cgatacctca taagccattt ttgtaattac ctcgccattt tcctgttttt
     1201 tctggtattc acggctctgt atccttcccc atattttgat attatcccct atctcaagcc
     1261 cggatgcata cctcgcattt ctaccccagc aaatacaagg tatataatcg gatttgttat
     1321 atggcctgtt aactgccaat aatatgtccg taatctctct accgaaggga gtagtcctgt
     1381 atattggctt cttacatata aatccattta aataaatctg attcggattt ttaattttcc
     1441 tctcatcttc aaaaaagtta acctctctgg caaataccgt cagaacaagc ctgcttccag
     1501 catcattgtt ataattgttg tatgaacgga attgcccctc aatctcaata aattggccta
     1561 tttccaattt ttcccttgag accagtctct ccgaaacggt aacggcaatc ctgtcatagc
     1621 tgtcactaag tcttgatact tccaaagtga aagtataaaa cccttctcca taaacctcat
     1681 gactgaattt tgattctgat actattttac cggaaatggt caccatgttg ttctcaatga
     1741 tgtttccgac catccgaccc ccacactcct ctcttaggct tcttacgtat atccgaaaat
     1801 ttatctaatg taatgtttat tcaactaagc tgccttttag aatcaattta ttactacatt
     1861 agccaatttt gtagtcattt cctgaaagaa actaattatt atatataaat ataccgtcaa
     1921 tagagctca
//
LOCUS       DAOOJC010000446         1926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_47511_length_1926_cov_3.997328, whole genome shotgun sequence.
ACCESSION   DAOOJC010000446 DAOOJC010000000
VERSION     DAOOJC010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47511_length_1926_cov_3.997328"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..564
                     /locus_tag="PLA01_10055"
     CDS             121..564
                     /locus_tag="PLA01_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01675.1"
                     /translation="MNDDFNKKLKQITEMLGGNEKASENLAGLLSLLADSGSKPQSTA
                     ASADTKVQKVKAESNTDSTESVPKLKVKDEETGRNNFQDNAEMMRTVKVIMDRMQNMN
                     DPRINLLTAIRPFLNNNRQKKLGNCIRLFQMTQLTKFMADSENTM"
     gene            578..940
                     /locus_tag="PLA01_10060"
     CDS             578..940
                     /locus_tag="PLA01_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01676.1"
                     /translation="MIYRRDPRFRRYSASVDESIPQPSGYEDDRSGGISRDIDSIPLP
                     NEKDETAFPNLHESSRKTSRPGIFTRRIQADEIILLVLIFLLIEEHIDDDFLLIILIY
                     LLVSEIIEEQFRSSQKPI"
     gene            complement(984..1847)
                     /locus_tag="PLA01_10065"
     CDS             complement(984..1847)
                     /locus_tag="PLA01_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01677.1"
                     /translation="MLALFFGLITLAIAIAITVGINSYFSFDLMSFSIFFILPLGSIV
                     VGTIASFGYHFGSYLSNKKIDSWRLLLGLVIPIIAFIGVRYGYYATAYLDDDMNLNYK
                     MIGEHVSNYSIEDSEEPLNFVSFTKLMVESSTISFTYRRRNMVNIEGKPVVNWIFFLI
                     DLIGSVVGSLMAMGLVIRGRNYCENCERYMKKQKELFSFPIGDVDTFIKMQDIKTLSS
                     ERIKSFLSCKASNNEEHYDVFINWCDKCFEGYINMKCFQRDSRGRLVENDKNNKDIKV
                     SSDIVKTFLGV"
BASE COUNT          670 a          397 c          346 g          513 t
ORIGIN      
        1 gggatcaagg agatgaaata gtatgcaaac tcaagagttt tctggaaaag gaataaaaca
       61 taccctgcgt tttatgatta agctggaaat ccaaggtggt taagggggga acaaacagga
      121 atgaatgacg acttcaacaa gaaacttaag caaattacag aaatgttagg cggtaatgaa
      181 aaagcatcgg agaatctcgc cggtttactg tctttacttg ccgactccgg cagtaaaccc
      241 cagtcaactg cagcatctgc cgatacaaaa gtgcaaaagg ttaaagccga gagcaatacg
      301 gactccactg agtctgtacc taaattaaag gtaaaggatg aggaaaccgg cagaaacaac
      361 tttcaggaca atgcagaaat gatgaggacg gtaaaagtca taatggatag gatgcaaaac
      421 atgaatgatc ccagaatcaa tttacttact gccataagac cgttcttgaa taataacagg
      481 cagaaaaaac tgggaaactg catcaggctt tttcagatga ctcagctgac caaatttatg
      541 gctgattcgg aaaatacaat gtgaaagggg cactacaatg atatatagaa gagatccgag
      601 atttagaaga tacagtgctt ctgttgacga aagcatcccg caaccatccg gctatgagga
      661 tgatagatca gggggcatct caagggatat agacagtatt cctctgccta atgaaaaaga
      721 tgaaactgct tttcccaacc ttcacgaaag cagcagaaaa acgtcccgtc caggcatatt
      781 tacccggaga attcaggcgg atgaaataat tcttcttgtc ttgatatttc tgctcatcga
      841 agaacatatt gatgatgatt ttctgttaat aatacttata taccttttgg tttcagaaat
      901 aatagaggaa caattccggt cgtcacaaaa gcccatataa aagcgaaggg acattatgtc
      961 ccccctcgct tttttagctg tttctaaact cccaaaaatg tttttacaat gtcggacgac
     1021 acctttatgt ccttgttgtt tttgtcattt tccaccaatc tgcctcttga atctctttgg
     1081 aaacatttca tattaatata tccctcaaaa cacttgtcac accaatttat aaacacatca
     1141 taatgctcct cgttgttgga tgctttacag gaaagaaaac tcttaatccg ttcggaactt
     1201 aatgtcttaa tatcctgcat cttaataaag gtatccacat ctccgatggg aaaactgaac
     1261 agttcttttt gtttcttcat atacctttca caattctcgc aataatttct gccccttatt
     1321 acaagcccca ttgccatgag acttccaacc acagaaccga taagatcaat cagaaagaat
     1381 atccaattaa ccacaggctt cccttctata ttcaccatgt tgcgtctgcg gtatgtgaaa
     1441 gatatggtgc tggactcaac cattaatttt gtaaaggata caaaattcaa aggttcctca
     1501 gagtcttcaa tggaataatt gctgacatgt tctccaatca ttttgtaatt caggttcatg
     1561 tcgtcatcca aatatgcggt ggcataataa ccgtaacgaa cacctataaa agcgataata
     1621 ggaatcacca atcccaataa aagcctccac gaatcaatct ttttattgga taaatatgag
     1681 ccaaaatgat aaccgaagct agcaatcgta ccgactacaa tagaacctaa aggcaatatg
     1741 aaaaaaatcg aaaaggacat cagatcaaaa gaaaaataag aattaatccc tacagtaata
     1801 gctattgcta tagctaaagt aatcaatcca aaaaataatg caagcatatt aaaaaagacc
     1861 cctttcaaaa aaataattaa tttcatccct tccggcaccc tctcgtgtcg ttgcccctaa
     1921 acgggg
//
LOCUS       DAOOJC010000447         1924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_47610_length_1924_cov_3.203852, whole genome shotgun sequence.
ACCESSION   DAOOJC010000447 DAOOJC010000000
VERSION     DAOOJC010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47610_length_1924_cov_3.203852"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="PLA01_10070"
     CDS             <1..842
                     /locus_tag="PLA01_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPD01678.1"
                     /translation="NLEGLKIAIDCANGAAYRVAPEALRELGAEVYVINNEPDGININ
                     RNCGSTHIKALQKFVVETGADVGLAFDGDADRVLAVDEEGKMVDGDQIMAIIGLGLKR
                     NGKLAKNTIVATVMSNLGFDIMAKREGINIVKTKVGDRYVLENMLENGYILGGEQSGH
                     IIFLEHNTTGDGLLTGVQLLSVLKSSGEKLSKLASVMQVLPQVLKNAKVKNENKNRYL
                     KDEVICKMCKELEEEFKGEGRVLIRPSGTEPLVRVMIEGKDQDYITRKAVEMVKVIEE
                     RLN"
     gene            1358..>1924
                     /locus_tag="PLA01_10075"
     CDS             1358..>1924
                     /locus_tag="PLA01_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HPD01679.1"
                     /translation="MCGIVGYIGNKTAVPILINSLKKLEYRGYDSAGVAVLNDANIKV
                     VKSKGRLSVLEEKLAADTPPGTIGVGHTRWATHGEPSDLNSHPHLSHSGSIAVVHNGI
                     IENYLELKEFLESQGYVFLSETDTEVVAHLIDYLYKGDLVKAVTEAINKIEGSYALGV
                     ICKDCADRFVAARKDSPLVVGLGKDENYI"
BASE COUNT          608 a          297 c          517 g          502 t
ORIGIN      
        1 gaaatctgga agggttaaag attgcaattg actgtgcaaa tggggcagca taccgggtgg
       61 ctcccgaggc tttacgtgag ttgggtgccg aagtttacgt gataaacaat gagcctgacg
      121 gtataaatat taatagaaac tgtggttcta cccacattaa agcccttcag aaatttgttg
      181 ttgaaacagg agccgatgta gggcttgcat ttgacggaga tgccgatagg gtactggcag
      241 tggatgaaga gggaaagatg gtcgatggtg accagattat ggctataata gggttgggct
      301 tgaaaagaaa cggtaagctg gcaaagaata ctattgtagc taccgttatg agcaatcttg
      361 ggtttgatat tatggcaaag cgggaaggca tcaatatagt caagaccaag gttggagaca
      421 gatatgttct ggagaatatg cttgaaaacg gatatattct tggaggagaa cagtccggac
      481 acataatctt ccttgaacac aatacaacag gagacgggct tttaacaggt gttcagcttc
      541 ttagtgtgtt gaagtcttcc ggggaaaaac tctcaaagct tgcttcggta atgcaggtat
      601 tgcctcaggt attaaagaat gcaaaggtaa aaaacgagaa caaaaacaga taccttaagg
      661 atgaagtgat atgcaaaatg tgcaaagagt tggaggaaga gtttaaagga gagggcagag
      721 tgctcattag gccttccgga acagagcctt tggtcagagt aatgattgag ggaaaggacc
      781 aggattacat taccaggaag gctgttgaga tggtaaaggt tattgaggaa agactgaatt
      841 gagtgtttct gaaaggtttg gatattgctt ttacggcagg attagagatg attcattaat
      901 gggtgaataa aggggggatt tttaagccca taatgaataa ggaaaaagcg cctgcacgtg
      961 gtattcaaca cgtagacgag gagaaggagg tcaccggaaa tctttatacc ggtgagatat
     1021 cgaaattatc ggcggaaacc ttccggttgt cacaaccgtt aagatcctac aaaacttaca
     1081 ggcaactgta aaacagagtg gatcggagtg aacaatcctg tcttttcata ttatcttaat
     1141 actagtttgc tatgcacaga tcccggacat atttgtaaca gatatgttcc agggtttatt
     1201 ctgtcttact ttgcaaaggg agtattgtaa gttaggccat ccattatata gcaaataata
     1261 acagaatatg tatagtttct taacaatgta aatctatgtg tgattacaga cattcttatt
     1321 gcaacatatg caggatacga tgaaaggaga ggttgagatg tgtggaatag ttggatatat
     1381 aggtaataag acagcggtac ctatattgat aaacagtctt aaaaagctgg aatacagggg
     1441 ttatgattca gcaggcgtgg cagttttaaa cgatgcgaat ataaaggtag tcaagagcaa
     1501 aggaaggctt tcggttctcg aggagaagct ggcagccgac actcctccgg gaacaatagg
     1561 agttggacat acccggtggg caacccacgg ggagccgagt gatcttaatt cgcaccccca
     1621 cttgagtcat tcaggcagta ttgccgttgt gcataacggg ataatagaga attatctgga
     1681 attgaaagag tttcttgaat cgcagggata tgtgttttta tccgaaacgg atactgaagt
     1741 ggtggcacac cttattgatt acttatacaa gggggacctg gttaaggcag taactgaggc
     1801 aataaataaa atagagggat cctatgctct tggagtaata tgcaaggatt gtgccgacag
     1861 gtttgttgct gccagaaagg acagtcctct ggtggtgggc ttgggaaagg atgaaaatta
     1921 tatt
//
LOCUS       DAOOJC010000448         1905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_48256_length_1905_cov_4.102162, whole genome shotgun sequence.
ACCESSION   DAOOJC010000448 DAOOJC010000000
VERSION     DAOOJC010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48256_length_1905_cov_4.102162"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..656)
                     /locus_tag="PLA01_10080"
     CDS             complement(114..656)
                     /locus_tag="PLA01_10080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPD01680.1"
                     /translation="MHKLCYYICLALYYFFARHLPASDAPYSLGSKQIRRFLCKRIFK
                     SAGKNINIEHGAFFASGREIEIGDNSGLGLNSRVTGPLKIGNDVMIGPDVMIFTQNHR
                     NDRLDIPMRLQTDPKRPVVIEDDVWIAARAIILPGVTVHKGAIVGAGAVVTKDVPEYA
                     VVGGNPARIIKYRDGRKVQP"
     gene            complement(708..1610)
                     /locus_tag="PLA01_10085"
     CDS             complement(708..1610)
                     /locus_tag="PLA01_10085"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HPD01681.1"
                     /translation="MSRSVVNTKKATVLDKVKGFISLVRPKQWIKNLFVFSALIFAKL
                     VFEPVYLRTVFLAFLSFCMISSCVYILNDIVDMEKDRLHPKKKTRPIASGLISKTEAW
                     IFLAVLLPVSVAAGFLLQVYFGLILVVYFVNNVLYSVAVKHMVILDVMSIALGFLLRV
                     IGGGVVIEVYISQWILLCTLLLSLFLGFSKRRNELVVLENGADSHRKILKEYSLEFID
                     NMLSIVTASTVMAYSLYTFSSKNNYFMMLTIPFVLYGIFRYQYIIYMKKEGGSPEETV
                     LSDIPLIVCIFLWVVISVIVLYLG"
BASE COUNT          613 a          456 c          292 g          544 t
ORIGIN      
        1 cataaaacgt tacagcagct atgtccaaag tcaataattt ctgttttcat cttctcagag
       61 cttattgtat catattttca gtctaagcct tcacattatt tcccggctaa atttcaaggc
      121 tgaacctttc tgccgtccct atacttaata atcctcgcgg gattccctcc cacaacggcg
      181 tattcgggca catccttcgt aacaacagcc cccgcaccca caatagcccc cttatgtacc
      241 gttactccgg gcaaaataat tgctctggct gcaatccaca catcatcctc gattactaca
      301 ggccttttgg gatcggtctg gagcctcata ggtatatcca gcctgtcatt tctgtgattt
      361 tgtgtaaata tcataacatc aggcccaatc ataacatcat ttccaatctt caaagggccg
      421 gtcaccctgg aattcaaccc caaccctgag ttgtctccga tttctatttc ccttccggat
      481 gcaaaaaaag caccgtgttc aatgttgata ttttttcccg cagatttaaa aatccttttg
      541 cataaaaacc ttcgtatttg ctttgagcca aggctgtaag gtgcatccga ggcaggcaaa
      601 tgccttgcaa aaaagtagta caaagcaaga catatatagt agcataactt gtgcataaac
      661 ccctccttga tacaaaattc tccaacaata aattctgccg tgagcatcta acccagatac
      721 agaactatta cgcttattac cacccacaga aatatgcaaa caattaaggg aatgtcggac
      781 agcacagtct cttcaggact tcctccctct tttttcatat atataatata ctgatatctg
      841 aaaatgccat ataaaacaaa gggtattgtc agcatcataa aatagttgtt cttgctggaa
      901 aaagtataaa gagaatatgc cataaccgtt gaggcagtaa caatcgaaag catgttgtca
      961 ataaactcca gagaatactc cttcagtatc tttctgtggg aatcggcacc gttttccaac
     1021 accaccagct catttctcct cttgctgaaa cccagaaaca atgaaagaag cagagtacac
     1081 aaaagtatcc attgggaaat atatacttca atgaccacgc cgcccccgat tactctaagt
     1141 aaaaagccta gggctatcga cataacatcc agtattacca tatgctttac tgcaacagaa
     1201 tataaaacat tattaacaaa gtacacaacc agaatcaagc cgaaatatac ttgcaataaa
     1261 aaacccgccg caactgaaac cggcagtaat accgcaagaa aaatccatgc ctctgtcttg
     1321 cttatcagcc ctgatgctat tggtcttgtc ttcttttttg gatggagcct gtccttttcc
     1381 atgtcaacaa tgtcgtttaa tatataaaca caggaggaaa tcatacaaaa gcttaaaaac
     1441 gccaaaaaca ccgttcttaa atatacaggc tcaaatacca gttttgcaaa tatcagagca
     1501 gagaacacaa aaaggttttt aatccactgc ttcggtctta caagacttat aaaccccttt
     1561 actttatcta aaacagttgc tttttttgtg tttaccacag accggctcaa taaaaatcac
     1621 tcctaaaaat aatagcccct aaacaactaa agcttttatt actcaataaa tattattgta
     1681 caatattccg gcaaaaaaac attttacatt cttgtttcaa atttatttgg gatttcagtc
     1741 tcatattata tcatttgatt gcactcattt ccagtgcact tttaatatag agggtgaaga
     1801 aaaatgctct agaaacttaa gttttgcatg caaaaagcct gtcaatactc agtggggccg
     1861 ggcaagactc acttcgtgaa aacgaatgcc cttcctttcc ccgtc
//
LOCUS       DAOOJC010000449         1901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_48421_length_1901_cov_2.854821, whole genome shotgun sequence.
ACCESSION   DAOOJC010000449 DAOOJC010000000
VERSION     DAOOJC010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48421_length_1901_cov_2.854821"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="PLA01_10090"
     CDS             <1..1267
                     /locus_tag="PLA01_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HPD01682.1"
                     /translation="YGGNIKGIISKLDYLIGLGINAIYLNPVFLSDSYHRYDIIDYYE
                     LDPVFGGKDELRELVDLCHSKGIKIIFDGVFNHTSDKFFAFRDVLEKGEASSYKQWYF
                     VDSFPAVNYPIPNYECFSFYGGMPKLNTANPETARYLFDVVKYWTLEFGVDGWRFDAA
                     DEVARKFWRDLRREIKALKKDVVLIGEIFDEASSWLEGDQFDTVLNYPVKEFINDLFA
                     YRSIDVELFKMRINSYIMKFNKNVLNSMVNIISTHDTPRFLTLCHGDKKRFELAVVFQ
                     FTFPGVPLVYYGDEVGMEGETDPDCRKPMVWDEGKWDRSILDLYRFLIETRKESIALR
                     VGEYSDLCTEGDKGVLAYKREYGTERIVVVMNTYNHNAGVTIELGNSFRGLARMEDLR
                     EKEFIPVEGCKIKLSLKPMEWRIYKAAEE"
     gene            1490..>1901
                     /locus_tag="PLA01_10095"
     CDS             1490..>1901
                     /locus_tag="PLA01_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HPD01683.1"
                     /translation="MKSEFKEYLKNEFGIDERVLRIADSALKEVASEFSAIDSVKEYN
                     QVKVIKAMQNNNVSDTHFNGTTGYGYGDKGRDGLDCVYRDVFKAEDALVRHQIVSGTH
                     ALALCLFGNLRPGDELLSVVGKPYDTLDEVIGIRG"
BASE COUNT          575 a          270 c          511 g          545 t
ORIGIN      
        1 atacggagga aatataaagg gtataatcag caagctggat tatcttatcg ggcttggaat
       61 taatgcaatt tatctcaatc ccgtgttttt atcggactct taccaccgtt acgatataat
      121 tgactattat gagcttgacc cggtgtttgg aggaaaggat gagttgaggg aattggttga
      181 cttatgccat agtaagggta taaagataat ttttgacggt gtattcaacc atacctctga
      241 taagtttttt gcctttagag atgttctgga aaaaggagag gcgtcttcat acaagcagtg
      301 gtattttgtg gactcttttc cggcagtgaa ctaccccata cccaactatg agtgcttttc
      361 tttttacggc ggaatgccga aactcaatac ggcaaaccct gaaacagccc ggtatctgtt
      421 tgatgtggtg aagtattgga cccttgaatt cggtgtggac ggatggaggt ttgatgcggc
      481 agacgaggtg gcccgtaagt tttggaggga cctcagaaga gaaataaagg ctttgaaaaa
      541 agatgttgtg cttatcgggg aaatattcga tgaggcgtct tcgtggcttg agggcgacca
      601 gtttgatact gtgttgaact atccggtgaa ggaatttatt aatgaccttt ttgcctacag
      661 gtccattgat gtggaacttt ttaaaatgag aataaacagc tacatcatga agtttaacaa
      721 gaatgtgtta aacagcatgg tgaacattat cagcacccat gacacaccga ggtttttgac
      781 tctttgccat ggtgacaaaa agagatttga attggctgtg gtgtttcaat ttactttccc
      841 gggagttccg cttgtttact atggggacga ggtgggcatg gaaggtgaaa cagaccccga
      901 ctgcagaaag cctatggtct gggatgaggg gaaatgggac aggagtattt tggacttata
      961 tagattttta attgaaacaa gaaaggaaag tatagcctta agagtcggag aatacagcga
     1021 tttgtgcaca gagggagata aaggggtatt ggcatacaaa agagaatatg ggacggaaag
     1081 gattgttgtt gtgatgaaca catacaatca caatgccggg gtgacaattg agcttggaaa
     1141 ctcatttagg ggacttgcga gaatggaaga tctcagggaa aaagaattta taccggtaga
     1201 gggatgtaaa ataaagctca gtttaaagcc tatggaatgg aggatttata aagctgctga
     1261 agaataattt atctataggt agtttattgt tttttgggca gaaaatatgg gtgaagcaaa
     1321 ttgaaggtgg tctgttaaga ctatggggag agggagagca ttttctttgc ggtcggttaa
     1381 tattgaaatt aaagcctgat gtgttaaaat aattattaaa caagattgaa cgaagatttt
     1441 gttaatgagg gcatagactt ataaaatata tttagtttgg ggagaaacga tgaaatcaga
     1501 atttaaagag tatttaaaaa acgaattcgg catagacgag agggtgctta gaattgccga
     1561 ctctgcattg aaagaggtcg cttcggagtt ttcagccatt gattcggtca aggaatataa
     1621 ccaggttaaa gtcattaagg caatgcagaa caataatgtg agtgatactc attttaacgg
     1681 tactacgggt tatggctacg gcgataaagg cagggatggc ttagactgtg tttacaggga
     1741 cgtttttaag gctgaagatg cattggtaag gcaccagatt gtatccggaa cccatgcatt
     1801 ggcgttatgc ttgtttggga atctaagacc cggtgatgag ctgttgtcgg tggtgggtaa
     1861 accttatgac acattggatg aagttattgg aataaggggt g
//
LOCUS       DAOOJC010000450         1887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_48936_length_1887_cov_3.938319, whole genome shotgun sequence.
ACCESSION   DAOOJC010000450 DAOOJC010000000
VERSION     DAOOJC010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48936_length_1887_cov_3.938319"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(304..1404)
                     /locus_tag="PLA01_10100"
     CDS             complement(304..1404)
                     /locus_tag="PLA01_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HPD01684.1"
                     /translation="MQRLKITGHQLYSLTANAAFGGSVIVIASVVAAIAKQDAWISAL
                     LTPILGMPVIWICWFLGSKYPGLTFVEIIKKILGRWVGTIVAASYISICLTLSYHVPW
                     YVGDFVTMQAMPETPASVINMLFIIAVVIATLYGIETIARASEIYFIFVLILSVLTVL
                     LVSPNAKFENLQPVLENGIIPVFKGSYVLSNFIIFPIVVVMMIYPAHIYNASEAKKSI
                     FKGFLFAGFITFITILMSLLVLGSKITAISEYPTYILAKEISLGIIFTRLEFIVAGTW
                     IITQFMMGTLVFYAAVRGISQLLGLKDHKKIVIPMGLIILVLSEIVFPDDIYQRNWVN
                     LVWLPYATTNGFILPILLFIVYLIKRMIKKAS"
     gene            complement(1416..1646)
                     /locus_tag="PLA01_10105"
     CDS             complement(1416..1646)
                     /locus_tag="PLA01_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01685.1"
                     /translation="MSILAVSVFSAAVCFVEIPKMVKQKLFRELWTFCILLGIGTILA
                     ILKSLDVKIPNPSDLIAWVYSPLAETMKSITK"
     gene            complement(1657..>1887)
                     /locus_tag="PLA01_10110"
     CDS             complement(1657..>1887)
                     /locus_tag="PLA01_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination C-terminal
                     domain-containing protein"
                     /protein_id="HPD01686.1"
                     /translation="NVKSLCEKALNKAQKELKTDIFGFGEEIHRKHPKLWKKLKKEWS
                     NKFPDLPVNITVEVETKQLGGIGKPLFMKEEE"
BASE COUNT          605 a          408 c          303 g          571 t
ORIGIN      
        1 tatctattaa aaacaatctc gtaatagggt ttagtactat ttacaagaac aaccgattta
       61 acgccatgtc ggctgtaaat atccaatgcc tttaccgctt cttgcggcga tatccccggt
      121 gtgattttat tataggtacc gtccacataa tattaagttt taatactatt ccttgtaata
      181 caagttttct atgcttactt gttgcgtaaa gagaagggca aatttgcttt tattacaaca
      241 aaccattcta ctgtctatta caatgtagta tttaaggtaa tactacaact gaaccagaca
      301 aagtcaagaa gcttttttaa tcattctctt tatcaaatac actataaaaa gcaggatagg
      361 cagaataaat ccgttcgtgg tggcgtaggg caaccaaaca agattgaccc aatttctctg
      421 atatatgtca tccggaaaaa ctatctccga caataccaat ataataagcc ccatgggaat
      481 aacgattttc ttatgatcct tcaatccaag aagctgtgaa atgcccctta cagctgcata
      541 gaaaaccagg gtgcccatca taaattgtgt aataatccat gtaccggcaa caataaattc
      601 aagccttgta aatatgatac cgaggcttat ttctttggca agtatgtatg taggatactc
      661 cgaaatggca gtaattttac ttcccaaaac cagtaacgac atgagaattg ttatgaaagt
      721 gataaagcct gcaaacaaaa aacctttgaa tatggacttt ttagcttctg aagcattata
      781 tatgtgtgcc ggatatatca tcatcactac gacaatagga aatattataa agtttgacaa
      841 aacgtatgaa cccttgaaca ccggaattat gccattttcc aaaacaggct gcaggttttc
      901 gaattttgca ttcggtgaaa caagaagcac agtcaaaacc gacaaaatta gaacaaaaat
      961 aaaatatatt tccgaagccc gtgctattgt ttctattccg tataatgtgg ctattacaac
     1021 cgctattata aacagcatat ttataacaga cgccggtgtt tcaggcatag cctgcattgt
     1081 cacaaaatcg cccacatacc aaggcacatg ataagaaaga gtcagacata tggatatata
     1141 gctggccgca acaattgtcc cgacccacct tcccaatatc ttttttatta tctcgacaaa
     1201 ggttaatccg ggatacttgc ttcccaaaaa ccaacatatc cagatcaccg gcataccaag
     1261 tataggagtc agtaatgccg aaatccatgc atcttgtttt gctatcgccg ctactacgga
     1321 agcaataact ataaccgacc ctccgaatgc tgcattggcc gtcaatgaat aaagctgatg
     1381 acctgtgatt tttaatcttt gcatattcaa acctctcatt ttgttatact tttcatagtt
     1441 tctgcaaggg gcgaataaac ccaagcaatc aaatccgaag gattgggtat tttcacatca
     1501 aggctcttga gtattgccaa tatcgtacct atcccaagca atatacaaaa agtccataat
     1561 tccctgaaaa gcttttgctt caccattttc ggtatttcta caaagcacac cgcagcactg
     1621 aacactgaca ctgccaaaat agacattata gccaagttat tcttcctcct tcataaaaag
     1681 cggtttacct atacccccca gttgtttggt ttctacctcc accgttatat taacgggaag
     1741 atccggaaac ttgttgctcc attccttttt aagttttttc cataattttg gatgctttct
     1801 gtgtatttct tcaccaaatc cgaaaatatc ggttttcagt tctttctgtg ctttgttcaa
     1861 ggctttctca cacaatgatt taacatt
//
LOCUS       DAOOJC010000451         1886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_48977_length_1886_cov_3.600765, whole genome shotgun sequence.
ACCESSION   DAOOJC010000451 DAOOJC010000000
VERSION     DAOOJC010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48977_length_1886_cov_3.600765"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLA01_10115"
     CDS             <1..542
                     /locus_tag="PLA01_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01687.1"
                     /translation="FYLIVGAAIVVLLVLTVVLLILKKRKKKKKTAVPGINYGNVSVF
                     FDENNNAFVIPYAKDKHGIGRAVGRPTFLKAPYNPLVLGQTVRSSMSLCKKRKAYPDS
                     KLMSSLKCKDWSEFSYKKRYISIYYKDRLGIVFNTTKRTQDGTYSLNFRGYEKVLSKE
                     AGDIELGIVIMNLLERCKC"
     gene            786..1073
                     /gene="gatC"
                     /locus_tag="PLA01_10120"
     CDS             786..1073
                     /gene="gatC"
                     /locus_tag="PLA01_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPD01688.1"
                     /translation="MKISKDTVEYVANLAKLDLTEEEKNRLMLDMERIILYFDKISNL
                     DTTQIVPTDYVIPVINVFRDDKACNFYDREEMLKNAPSKEKGFFKVPKIFE"
     gene            1173..>1886
                     /locus_tag="PLA01_10125"
     CDS             1173..>1886
                     /locus_tag="PLA01_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HPD01689.1"
                     /translation="MEIHELTVHEMGELIKSKKVSAVELTQLMLDRINEIDKEVNCFI
                     TVLNDEAIIRAKTVQERIDKGEVRSPLAGIPMAVKDNICTRGIKTTCASKMLENFVPP
                     YDATVIEKLYYKDTVLLGKLNMDEFAMGSSTENSHLKVTRNPWDLNRVPGGSSGGAAA
                     AVASGLTGFALGSDTGGSIRQPAAFCGVVGIKPTYGTVSRFGLVAFASSLDQIGAITR
                     DVTDCALVLNAITGYDSMDS"
BASE COUNT          590 a          294 c          458 g          544 t
ORIGIN      
        1 tgttctattt aattgttggt gctgcaatag tggtgttgct tgtactcacc gtagttttgt
       61 taatcttaaa gaaaagaaaa aagaaaaaga agacagcagt gccaggtatt aattacggaa
      121 atgtgagcgt tttttttgac gagaataaca atgcatttgt tataccctat gccaaagata
      181 agcatggtat tggaagagct gttggaagac ctacgttttt gaaggctccg tataatccgt
      241 tggtacttgg tcagactgtt agatcatcca tgtctttgtg taagaaaagg aaagcctatc
      301 cggacagcaa gctcatgagt agtttgaagt gtaaggattg gagcgaattt tcctataaga
      361 aaaggtatat ttccatatac tacaaagata gacttggaat agtgtttaat acaacgaaaa
      421 ggacacagga cggaacgtac agtttaaatt tcagaggcta tgagaaggtt ttgagcaaag
      481 aagcaggaga tattgaactt ggaattgtaa ttatgaatct tttggaaaga tgtaaatgct
      541 agaaagtgta tgaacactta atcttattaa catatgtttt acccttaacg ggaatgggcg
      601 gtcttgcaaa gatcgccttt ttatatgtgt gcgtccagcg aagctggact acaaaattca
      661 tcgataatac cttttttagg attctcttaa agtcattggg ataatcacct gttaatacga
      721 catggaaagg gagaacattg tttgatataa tatttcattg aggcataaag cggggaggaa
      781 agggtatgaa aatttcaaag gataccgtgg agtatgttgc aaaccttgct aagcttgatc
      841 ttacagagga agaaaagaat aggcttatgc ttgatatgga aagaattatt ttgtactttg
      901 ataaaataag taatcttgat acgacgcaaa ttgtgccgac cgattatgta ataccggtaa
      961 taaacgtttt ccgggatgac aaagcctgca atttttatga ccgggaagag atgcttaaga
     1021 atgcaccttc taaagagaag ggatttttta aagttccgaa aatatttgag taaaggattt
     1081 tggcagaaag ccgggaatat agtatgcctt ccggtataat gtacatatcg gcaataataa
     1141 ggtattgtca attgattaag gaggatttta ggttggaaat tcacgaactg acagttcatg
     1201 agatggggga acttataaaa agcaaaaaag tgagtgcagt ggaactcact caattaatgc
     1261 ttgacagaat taatgaaatt gataaagagg ttaactgctt cataacggtt ctcaatgatg
     1321 aagccataat cagagcaaaa acggtgcagg aaaggattga caagggcgag gtacggtcac
     1381 ctcttgcggg aattcctatg gctgtaaaag ataatatatg cacccggggt ataaaaacca
     1441 cttgtgcttc gaagatgctt gaaaattttg ttcctcctta cgatgcaacg gtaattgaaa
     1501 agctttacta taaagatacc gtacttctgg gcaaattgaa tatggacgag tttgccatgg
     1561 gaagctctac tgaaaactca catttgaagg ttacacgcaa tccatgggat cttaaccggg
     1621 ttccgggagg atcaagcggt ggggctgctg cagcggttgc ttccggattg acggggtttg
     1681 cactgggttc ggacacagga gggtccatta ggcaaccggc agcattttgc ggtgttgtag
     1741 gtataaaacc tacttatggt acggtgtcac gttttggttt ggtggccttt gcgtcttccc
     1801 ttgaccagat tggggctatt acccgggatg tgaccgattg tgcccttgtg ttaaatgcca
     1861 ttaccggcta cgactcaatg gattcc
//
LOCUS       DAOOJC010000452         1852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_50321_length_1852_cov_3.498609, whole genome shotgun sequence.
ACCESSION   DAOOJC010000452 DAOOJC010000000
VERSION     DAOOJC010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50321_length_1852_cov_3.498609"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLA01_10130"
     CDS             complement(<1..214)
                     /locus_tag="PLA01_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPD01690.1"
                     /translation="MANYRVDLANLDNVIKRTIEAISSSKTELYEIAEGARDECRRLE
                     EELEHIKELISETTESVELLEEKLKES"
     gene            461..1732
                     /locus_tag="PLA01_10135"
     CDS             461..1732
                     /locus_tag="PLA01_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPD01691.1"
                     /translation="MKSKVAIIKTNPSTVLEDYHRLMNLADYQDYIAKDADTALKINI
                     SWHFFYPGSSTTPWQLEGVIRAMKRDGYKPDLIHGCHNRTVVIDSHLGERENKQINVI
                     NSHGLRNIHLYEGEEWIDIKEAVGDLAKKFLCLNEVYPKGFSIPKRFIGENIIHLPTV
                     KTHVFTTTTGAMKNAFGGLLNEKRHWTHPVIHETLVDLLMIQKKIHRGVFAVMDGTFA
                     GDGPGPRCMIPHVKNVILASADQVAIDAVAAKLMGFDPLKDLKYIRLAHDAGLGCGDV
                     SQIEIVGDLDALDEKWNFVGPFKKMTFASKCQHLIYWGPLKKPVEWSLKTILAPWSYI
                     ASVIYHDLYWYPKHYSRVKEISNSDWGRLFANWEQLELPPDNLSVPGWDSVGDKPLEL
                     SEETKDMIKKGFKVLGGAIKEAPEFKKKPKK"
BASE COUNT          566 a          372 c          384 g          530 t
ORIGIN      
        1 tgctttcttt aagcttctcc tcaagaagtt cgacactttc cgtcgtctca gatatgagtt
       61 ctttgatgtg ctcaagttcc tcttcaagcc gcctgcattc atcccttgcc ccctcagcaa
      121 tctcatacag ctcggtctta ctgctgctta tggcttcaat agttctcttt ataacattgt
      181 caagatttgc taaatcgact ctatagttag ccactttgat acctcaccga ataatttgac
      241 taataatatt attattctac attaactctc aaattccttt attaaattgc actttgtgaa
      301 attattacca ataattattt tgtttcacac ttatccgtat gctttttggg cggagaagta
      361 agtatgaacc caatagatta ctaaccctgt ctataagttt taaaatatgt tataatgtat
      421 atcgttcttt atataacaat catagaaagg aaaacaaagg atgaaatcaa aagttgcaat
      481 aattaaaaca aatccgtcca ccgttctgga ggattaccat agattaatga acctcgcaga
      541 ttaccaggat tatatcgcaa aagatgcaga tacggcacta aaaataaaca tcagttggca
      601 ctttttttac cctggaagct ccaccacgcc ctggcagctt gaaggcgtta tcagggccat
      661 gaagcgggat ggatacaaac ctgatctgat ccacggctgt cataaccgaa cggtggtaat
      721 cgattcgcat ttgggagaaa gggaaaacaa acagataaac gtcattaatt cccatggact
      781 tcgcaatatc cacctctatg agggtgagga atggattgac ataaaagaag ccgtaggaga
      841 ccttgcaaaa aagtttttat gtcttaatga ggtttatccg aagggctttt caatcccaaa
      901 aaggttcatc ggtgaaaaca ttatccacct ccccacggtt aaaacccatg tattcaccac
      961 cacaaccggt gccatgaaaa atgcctttgg cgggctgttg aacgaaaaaa ggcattggac
     1021 acatcctgtc atccatgaga ctttagttga tttattaatg atacaaaaga agattcaccg
     1081 gggtgttttt gccgttatgg acggaacttt tgccggagac ggtccaggtc cccgttgtat
     1141 gataccccat gttaaaaatg taattctggc atcggcggat caggtggcaa tcgatgcagt
     1201 tgccgcaaaa ttaatgggat ttgacccgct aaaagattta aagtatattc gattggccca
     1261 tgatgccggt ctggggtgcg gtgacgtaag tcagatcgaa attgtcggag accttgatgc
     1321 cttggacgag aaatggaact ttgtgggacc ttttaaaaag atgacttttg cgagcaagtg
     1381 tcagcacttg atttattggg gaccgttaaa aaaaccggtg gaatggtcat taaagactat
     1441 tctggcccca tggtcctata ttgccagcgt tatttaccat gatctgtact ggtatcccaa
     1501 acattacagc agggtaaagg aaatctccaa ttcggattgg gggcgtttgt tcgccaattg
     1561 ggagcaactg gaattacctc ccgacaatct gtcggttccg ggatgggatt ccgtcggcga
     1621 caagcctttg gagctttccg aagagacaaa agatatgatt aaaaaaggct ttaaggttct
     1681 tggcggtgca ataaaagaag ctccggaatt caagaaaaag ccgaagaaat aagagcatta
     1741 agcgattcga aagtaagaat agtaaaatct tatatgctgc atctattata aatttatata
     1801 atagagtaga gaaataattg ctaagaatta tttccctctc gttgaaccgt ac
//
LOCUS       DAOOJC010000453         1839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_50896_length_1839_cov_2.925448, whole genome shotgun sequence.
ACCESSION   DAOOJC010000453 DAOOJC010000000
VERSION     DAOOJC010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50896_length_1839_cov_2.925448"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PLA01_10140"
     CDS             complement(<1..576)
                     /locus_tag="PLA01_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01692.1"
                     /translation="MSKIQAYIEKLHNLENWDEYLMQESALPGPRANLELIQAVAEVG
                     DESTFLHLLSYTPDMAPVNTQQGYLACCGTVGLGKLVKSGKTKYLERLRLLASDPRWR
                     IRECVAMALQIYGDEHIEALITEMEMWAEGNNYEKRAAAAALCEPRLLKHKEQVSKVL
                     QILDSITCSIKGITDRKDEGFIALKKGMAYCW"
     gene            complement(771..1472)
                     /locus_tag="PLA01_10145"
     CDS             complement(771..1472)
                     /locus_tag="PLA01_10145"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HPD01693.1"
                     /translation="MIKGSIGILAGMGPRSTTPFLELVLDQCQVQYGAKYDIDYPHII
                     VYSLPTPFYIDREIDHSLMKRTIIDGLKKLESTGVSFIAMPCNSAHLYFDELKNSIKA
                     PLLNIVEETVSCLPNSKQRTTIFATEITVKSELYQKGIISAGHEFIFMEQWQTKVNNI
                     INAIKMKRDREFINSLWNELICEVKDQCVDSVIIACTDLSILSNVAELKLNIYDSSEA
                     LAKSVVKKYLKSRQE"
     gene            complement(1538..>1839)
                     /locus_tag="PLA01_10150"
     CDS             complement(1538..>1839)
                     /locus_tag="PLA01_10150"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HPD01694.1"
                     /translation="IRVDLSQGNERYTLLNGQEKELAPGDMMLVDGSGIISGIVYGPD
                     KRTQITQNTKNVLFVVYAPPGINEDLLEQHLQDINQYIKIVSPDAIIETQKVYRI"
BASE COUNT          489 a          413 c          301 g          636 t
ORIGIN      
        1 ccagcaatat gccattccct ttttcaacgc tataaatcct tcgtcttttc tatccgtaat
       61 cccctttatt gaacatgtga tactatcaag tatttgtaac acttttgata cttgttcttt
      121 atgctttaat agtcttggtt cgcaaagtgc agctgccgct gctctttttt catagttatt
      181 gccttctgcc cacatttcca tttctgttat cagtgcttca atatgctcat ccccataaat
      241 ctgaagtgcc attgcaacgc attcacgtat acgccaccga gggtcagagg ctagcaaccg
      301 caaacgttca agatatttag tctttccgct ttttacgagt tttcctaatc ctacggttcc
      361 gcagcaagct aaatatcctt gttgtgtatt gacaggagcc atatctggtg tatagctgag
      421 caaatgcaga aatgtactct catccccgac ttctgcgaca gcttgtatta attctaagtt
      481 agctcttggg cctggtaaag cagattcttg cattaagtat tcgtcccaat tctccaagtt
      541 atgtaatttc tcaatatatg cctgaatttt gctcatatcc ttctctcctt tttcaaatat
      601 aggaaccgtt ttcgtataac ggcttcgaac tcacgacacc actgaaccgg acttcagagc
      661 ccttcccccc tggcggggcg acacctcccg gtgaaggagt gtggcagcat gagtgcattg
      721 ttaggcggtc gtgggtcatc atctataaaa gagttcaggg ggtaatccct ttattcttgc
      781 ctgcttttta gatatttctt aactacagat tttgctaaag cttccgacga atcatatata
      841 ttcaatttca gctctgcgac attactcaaa atacttaaat cagtacatgc aattattaca
      901 ctgtctacac attgatcctt aacttcacag ataagctcat tccataatga gttgataaat
      961 tctctatccc gcttcatctt tattgcattt ataatattat ttaccttcgt ttgccactgc
     1021 tccatgaaaa taaactcatg tccggcagat ataatacctt tctggtacag ttcagactta
     1081 acagttattt cagtggcaaa tatcgtggta cgctgcttac tattcggcag acaacttact
     1141 gtttcttcca caatgtttaa taagggtgcc ttaattgagt tcttcaactc atcaaaatac
     1201 aaatgtgccg aattacaagg catggctata aagcttactc ctgttgattc cagctttttc
     1261 aaaccatcta ttatagttct tttcattaat gaatggtcta tttcccgatc aatgtaaaac
     1321 ggagttggaa gtgaatatac tataatgtgt ggataatcta tatcatactt tgcaccatat
     1381 tgtacttgac attgatctag aactaattcc agaaagggag tggttgatcg tggtcccatc
     1441 cctgctaata ttccgatact gccttttatc atattaacct ccatttttaa gtaaatacgt
     1501 gaagccatgg ttatccgaag cggccaagta aatcctatca aattctatat actttttgtg
     1561 tttctattat ggcgtccggt gaaacaattt ttatatactg attaatatct tgcagatgtt
     1621 gctctaacaa gtcttcatta atgcctggcg gagcatatac aacaaataaa acattctttg
     1681 tattttgcgt tatttgggtt cttttatcag gcccgtaaac aataccggat atgatcccgc
     1741 tcccatcaac cagcatcata tcacccgggg ctaattcttt ctcttgtccg ttcaataatg
     1801 tataacgttc atttccttgt gataaatcta cccttattg
//
LOCUS       DAOOJC010000454         1828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_51319_length_1828_cov_3.993232, whole genome shotgun sequence.
ACCESSION   DAOOJC010000454 DAOOJC010000000
VERSION     DAOOJC010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51319_length_1828_cov_3.993232"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1694
                     /locus_tag="PLA01_10155"
     CDS             <1..1694
                     /locus_tag="PLA01_10155"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HPD01695.1"
                     /translation="YLKTLQNYISETLLTRKDVIKLGIDICSAIETCSQRGIIHRDIK
                     DENIFVSMYGEFKLGDFGISKELSKSGRAASMKGTPLFMAPEVFRGDKYDVRADLYSL
                     GIVLYRLLNCGRIPLVPPYPQVIKFKDSEEALERRISGEDLNPPVEAGDKLGAVILKA
                     CMHNPSERFSNATEMRQELERVLDSLGTEEKNQCLTLPGYKKRNEKQRDAEFDKTEVY
                     PEFVPEEQKKSPMDLDDIFFEDDIFEDDFFEDILEQVNGDDAFDGSQQRDIKVSRSSR
                     GNSQGNIINGGLVSSQGLWDYYSITNKLCKVKIDGSFEQVLANISAWYINVLGDWVYF
                     SNSGDDDSIYRISIDGAYVEKLNSDKAWDITVKDDWIFYSNESDGYGIYKIKTDGSCR
                     TKISSDKSYCLNVTKDWIYYVNKNDKGSIYKIKLDGTERVKLIDNDCNYINAEDGCIY
                     YANNSDGGRIYKMTLDGNVVRINEDTSSNINVYGEFIYYSNKNDGGKLYRINKDGSNK
                     TLLCTNSCDFINITENWIYYVNKEDGGNLYKIKFDGSNKVKLSGNTDEDGEWFYL"
BASE COUNT          631 a          262 c          417 g          518 t
ORIGIN      
        1 agtaccttaa aacacttcaa aattacatca gcgaaacatt gctgacaagg aaggatgtaa
       61 taaagctcgg tatagatatt tgttctgcaa ttgagacctg ttcgcaaagg gggataatac
      121 atagagatat taaagacgaa aatatttttg tatcgatgta tggagagttt aagctaggtg
      181 attttggtat ttcaaaggaa ttgtcaaaga gcggaagagc ggcatcaatg aaagggacgc
      241 cgctttttat ggctcctgaa gtgtttcggg gcgataaata tgatgtcaga gccgatttat
      301 actctttggg aattgttctg tacagattgc tgaattgcgg cagaataccc cttgttccac
      361 cttatccgca ggttataaag tttaaggata gtgaagaagc tttggagaga aggatttccg
      421 gtgaggattt gaatcctccc gttgaagcgg gagataagct tggagcggtt attcttaagg
      481 catgcatgca taatccttca gaacggtttt caaatgctac ggaaatgagg caggaacttg
      541 aaagggtttt ggattctctg ggcaccgaag aaaaaaatca gtgccttacc cttcccggat
      601 ataagaaaag aaatgaaaaa cagagggatg cagaattcga taaaacagag gtatatcctg
      661 aatttgttcc ggaagaacag aaaaaaagcc ctatggatct ggatgatatt ttttttgaag
      721 acgatatttt tgaagatgac ttttttgaag atatattgga acaagttaac ggggatgatg
      781 cttttgacgg atcccaacaa agggatataa aggtatcaag aagcagcaga ggcaattcgc
      841 aaggtaatat tataaacggc gggctggttt cgtcgcaggg tttgtgggat tactactcta
      901 ttacaaacaa gctctgtaaa gtaaaaattg acggcagttt tgaacaagtg ctggcaaaca
      961 tatccgcatg gtatataaat gttttgggcg attgggttta tttcagcaat tccggtgatg
     1021 atgattcgat atacagaatc agcattgacg gagcctatgt agaaaagtta aacagtgaca
     1081 aagcctggga tataacagtg aaagatgatt ggattttcta tagcaatgaa agtgatgggt
     1141 atggaatata taagattaag accgatggca gctgcagaac gaaaataagc agcgacaagt
     1201 catattgctt aaatgtaact aaggattgga tttattatgt taacaaaaac gataagggga
     1261 gcatatataa aataaaactg gacggtactg aaagggtaaa gttaattgat aatgattgca
     1321 attatataaa cgctgaggac ggctgcatat attatgcaaa caacagcgat gggggaagaa
     1381 tctacaaaat gactttagac ggcaatgttg taaggattaa tgaggacact tcatcgaata
     1441 ttaacgtata tggtgaattt atatattatt ccaataaaaa tgacggaggc aagttataca
     1501 gaattaataa ggatggcagt aacaaaacac tgctttgcac gaacagctgt gattttataa
     1561 acatcaccga aaattggatt tattatgtca acaaagaaga cggaggaaac ctgtataaga
     1621 ttaaattcga tggcagcaat aaggtcaaat taagtggaaa taccgatgaa gacggtgaat
     1681 ggttttattt ataaaacttt tttgaacttc ttttttatat tcattttttt attttaaaat
     1741 tatcgttcat caaagcttat atcgtcatac ctagaaccca ccaccgttat ttctatatta
     1801 tccaggacaa caccctttag tgtgggaa
//
LOCUS       DAOOJC010000455         1788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_53056_length_1788_cov_2.654357, whole genome shotgun sequence.
ACCESSION   DAOOJC010000455 DAOOJC010000000
VERSION     DAOOJC010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53056_length_1788_cov_2.654357"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLA01_10160"
     CDS             complement(<1..738)
                     /locus_tag="PLA01_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPD01696.1"
                     /translation="MEKEIYLDNSATTRPYDEVVDYIDDVNRNYYGNPSSLHTKGIQA
                     EKLVRNSRDIIARTLGAARDEIYFTSGGTESNNLAIAGYLYANPRKGKHIITTKTEHP
                     SVLEVFRHMADQGYNVDYLDVDSKGSVRIDDIERLVNEDTSLISIIYINNETGAIQPI
                     DEIAAVIKRIGKDIALHVDAVQAYGKLNIIPKNQGIDLLTMSSHKIHGPKGVGAIYIN
                     KGIKVKPLILGGGQESLLRSGTENVPGI"
     gene            complement(970..1632)
                     /locus_tag="PLA01_10165"
     CDS             complement(970..1632)
                     /locus_tag="PLA01_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPD01697.1"
                     /translation="MSGYNLDENKGLLNLFGNKNLMKLIRPGSPIDVKHFKMYEYINC
                     TVLEKNENDLKLEMKDHTQETLFFPGDHVVLNYSNNDELYVIDGTIIYVFSINPPVVK
                     IRMNKCEKLKNLRKHERYYVSLMANIRVSGGNKPFFAVVKNMSSGGIKINCNERLMPD
                     DVLEVEVILDRTNKLVFSGAVVRKNKLGNYFEYGIEIKGISESNIKCLYHYLKWLGSE
                     YR"
BASE COUNT          535 a          391 c          263 g          599 t
ORIGIN      
        1 aattcccggc acattttcag ttccggacct taaaaggctc tcctgccctc cccccaaaat
       61 caggggttta accttaatac ccttatttat atatattgcc ccaacacctt tcggtccatg
      121 aatcttatga gagctcattg tcaataaatc aattccctga ttcttaggaa taatatttaa
      181 ttttccgtat gcttgaaccg catccacatg cagtgcaata tcctttccga ttctttttat
      241 aaccgctgca atttcatcaa tgggttgtat tgcaccggtt tcattattaa tataaatgat
      301 acttatcaga gaagtatcct cattcacaag cctttcaata tcatcaatgc gaaccgaacc
      361 ctttgaatcc acatccaggt agtcaacatt atagccctga tccgccatat gtctgaacac
      421 ttccagaact gaagggtgtt ctgtctttgt tgttatgatg tgcttgcctt ttcggggatt
      481 ggcataaaga tagcccgcta tagcaaggtt gtttgactcg gtacccccag atgtgaaata
      541 aatctcatcc ctggctgcac caagggttct tgcaataata tccctggagt ttcttaccag
      601 cttctcggct tgaattccct ttgtatgtaa ggatgaagga ttaccgtaat aatttctgtt
      661 tacatcatca atataatcaa caacttcatc atagggcctt gtagtggcac tattgtccag
      721 ataaatttct ttttccattt tatatcctct ctgttatatg cttctgtacc ggttaccggc
      781 aactgtcgca aaccaaccgt acctaaaata ttctaacaca tattatatag tttaaccaat
      841 ataatctgtt tttttgtttt atattaattt tgagaggata ataatggcaa taaaccaatt
      901 cacagacagg aagatgccaa gataagacaa aaagcccaca aattatttgg agctaaaata
      961 agatatgatt tacctatatt ctgaaccaag ccactttaaa tagtggtaaa gacattttat
     1021 attagactcg gaaattccct taatttcgat tccgtactca aagtaatttc ctaatttgtt
     1081 ttttcttact accgctccgc tgaagacaag tttgtttgtc ctatcaagaa taacctcaac
     1141 ctcaagtaca tcatcgggca tgagtcgctc attacagtta atcttaattc ctccggaact
     1201 catgtttttt acaacagcaa agaaaggttt attacctccg gaaaccctga tattggccat
     1261 aaggctgaca taatacctct catgcttcct cagattcttc agcttttcac atttattcat
     1321 tcgaatttta actacaggag gattaatact aaagacataa ataatagtac cgtctataac
     1381 ataaagctcg tcattgtttg aatagtttaa aacaacatga tccccgggaa agaaaagagt
     1441 ctcctgcgta tgatccttca tttcgagctt aagatcattt tcatttttct caagcactgt
     1501 acaattaatg tactcataca ttttaaaatg ctttacatct attggggatc ccggtctaat
     1561 aagtttcata agattcttgt ttccaaaaag gttaagtaat cccttatttt catccagatt
     1621 atatcctgac atatcggtgc ccctttcacg ttgaattctg aatcttttcc cgaacaataa
     1681 aatctcagga gtaataaacc ccttcagtat ctgtccctaa tattttgtgg gatcatatct
     1741 aataacacaa tatgttctaa atcagctacc tcttatatca attagcca
//
LOCUS       DAOOJC010000456         1762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_54244_length_1762_cov_3.072642, whole genome shotgun sequence.
ACCESSION   DAOOJC010000456 DAOOJC010000000
VERSION     DAOOJC010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54244_length_1762_cov_3.072642"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="PLA01_10170"
     CDS             <1..1287
                     /locus_tag="PLA01_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HPD01698.1"
                     /translation="ENNGAALTARVMEPLAGSSHILGLGEVMNYPGVLAGDKEILNKI
                     ELYDNAVVDGHSSGLSEHELCAYKAVGVLTNHECTNIDEVIAHLRLGMYVQIREGSAA
                     RNLDDIISGLIERGVPLNHCLFCTDDKHLQDISREGHISFNVKKAISLGVDPIEAIKM
                     ATINPAKCYHLKRLGAVAPGYSADLLILSDLNNFDIDKVLFRGSVVCEKGKKPNISRI
                     IDDPNVLNTVRIPNLSPEHLKIRIKDDTANVITIIPNQLITKKERLAVPKDNENNFIP
                     DEVFAKIAVVERHKATGNIGLGIVKGFGIRNGAIASTVAHDSHNLIIIGDNDADMLLA
                     AKVLKEANGGYTVVSGGEVKGTLPLPIAGLMSDRDAFFVENKLAGMIESAKSLKVPDG
                     IDPFVTLSFLALPVIPELRVTDRGLFDVESFSFIDI"
     gene            1337..>1762
                     /locus_tag="PLA01_10175"
     CDS             1337..>1762
                     /locus_tag="PLA01_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPD01699.1"
                     /translation="MDLIIKNGTIITASESYKADITVKNGKIAMISENIQATGERVVD
                     AAGKLVLPGAIDAHVHLELPVGETVSADSYESGTRAAACGGTTMVFDFVTQEKGMGLV
                     ETADSRLGYVESRACVDYAFHIAITDLTEKVLDEFKASVE"
BASE COUNT          518 a          362 c          449 g          433 t
ORIGIN      
        1 gaaaacaacg gagcggctct tacggcaagg gtaatggagc ctcttgcggg cagctcccat
       61 atacttgggc tcggagaggt tatgaattac cctggagtgc tggccgggga taaggaaatt
      121 ctgaacaaaa tcgagcttta tgataatgcg gttgtagacg ggcattcctc gggattatcg
      181 gagcatgagc tttgtgcgta caaggctgtc ggtgtactga ccaaccatga atgcacaaat
      241 attgatgagg ttatcgcaca tctgaggctt ggcatgtatg tgcagataag agaaggctct
      301 gccgccagaa accttgatga cataatatca gggcttattg aacggggcgt tccgctgaac
      361 cactgtctct tttgcactga cgacaagcac ctgcaggata tttccaggga aggacatata
      421 agcttcaatg taaaaaaggc aataagcctt ggtgtagacc ctattgaagc aataaaaatg
      481 gcaacaataa atccggcaaa gtgctaccat ttaaagcgtt taggagctgt agcccccgga
      541 tattcggctg atttgcttat actttccgat ttaaacaact ttgatattga taaggtgctt
      601 ttccgtgggt cggttgtttg tgaaaagggc aaaaagccta atatcagcag aatcatagat
      661 gacccaaatg tgttaaacac agtaagaatc cccaacttat cccctgagca cctgaaaata
      721 agaattaaag atgatacggc aaatgttata accatcattc ctaatcagct gattacaaaa
      781 aaagaaaggc ttgcagtacc caaggacaac gaaaacaact tcatacccga tgaagtgttt
      841 gcaaaaatcg ccgttgtaga gcggcacaaa gctaccggaa acatcgggct tggaattgta
      901 aaaggctttg gtatcaggaa tggtgccata gcatccaccg tcgcccacga ttcccataat
      961 cttataataa taggtgataa cgacgctgat atgcttctgg ctgcgaaagt actgaaggaa
     1021 gctaacggag gttataccgt tgtgagcggc ggagaggtaa aaggtacgct gccgctgccc
     1081 attgccggcc taatgagtga cagagatgca ttttttgtgg aaaacaagct ggcgggcatg
     1141 atagagtcag caaaaagcct taaagtacct gacggaattg atcccttcgt aaccctgtcg
     1201 tttcttgcac tgccggttat ccccgagtta agggtaacgg acaggggcct ctttgatgtg
     1261 gaaagcttca gttttattga tatatagcta tttgcaaaat aacggataaa aggaagtgcc
     1321 ttttatgagt gaaaatatgg acttaataat caaaaatggg acaatcatta ctgcttcgga
     1381 atcatacaaa gctgatatta ccgtgaaaaa cggcaagatt gcgatgattt ccgagaatat
     1441 acaggcaaca ggtgaaaggg ttgttgatgc cgccggcaaa ttggtactgc cgggtgccat
     1501 tgatgcccat gtgcatctgg aactgcctgt cggagaaacc gtctcggcgg acagctacga
     1561 gtccggtaca cgggctgctg cctgcggagg tacaacgatg gtattcgact ttgttaccca
     1621 ggagaaaggc atggggcttg tggaaacagc agacagcagg ctgggatatg ttgagtcccg
     1681 ggcttgcgtt gattatgcct ttcatattgc catcaccgac ctgaccgaaa aagtccttga
     1741 tgaattcaag gcttctgtcg ag
//
LOCUS       DAOOJC010000457         1759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_54402_length_1759_cov_3.956573, whole genome shotgun sequence.
ACCESSION   DAOOJC010000457 DAOOJC010000000
VERSION     DAOOJC010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54402_length_1759_cov_3.956573"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..>1759
                     /locus_tag="PLA01_10180"
     CDS             316..>1759
                     /locus_tag="PLA01_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPD01700.1"
                     /translation="MKKMIVFLLTVALLTVLLIPQGMVIHAADYNYGEALQKAIMFYE
                     FQRSGKLPENTRNNWRGDSALNDGADNGLDLTGGWFDAGDHVKFNLPLAYTVTMLAWS
                     VYESRDAYEKSGQLPYILDNIKWATDYLIKCHPSPNVYYYQVGDSALDHSWWGPAEVL
                     PMARPSFKVDTSNPGSTVVAETAAAMAASSIIFKSTDPSYSATLLMHAKDLFTFADTT
                     RSDTGYKEAEGYYSSHSGFYDELTWASAWLYLATGDDAYIKKAESYKPNWETERGTDI
                     IKYSWAHCWDNKSFGAFLLLARITGDSFYKQCIESNLDWWTTGHNGTKVQYTPKGLAW
                     LDGWGSLRYATTQAFLASVYADWDGCDPSKASKYQEFAKSQVDYALGSAGRSFVVGFG
                     TNPPKNPHHRTAHGSWCAMMDTPPQSRHVLVGALVGGPGSSDNYVDSLSDYQCNEVAN
                     DYNAGFVGVLAKMYDKYGGTPIPNFVAYETP"
BASE COUNT          513 a          319 c          422 g          505 t
ORIGIN      
        1 agcgaagttt ttcgctgttt ttttatatat ttttcagctt aaataatatg tggcattgtt
       61 taattaatat tttgaatttt tgacgttttg atgatagaat aaaagtaggt acggtttggt
      121 agtgttgaaa tcaataatgc taagcagcat atctatgcaa tttatattct tgtgattgat
      181 atcattccgt tttaatataa taaactgtac aaaacacaga tataaaaaat aacatgtgtt
      241 agaatttggc gtatcggcag gatgtgagga tatgccaata aaatattaat attaaagtag
      301 aaggggagat tgatagtgaa aaagatgata gtatttttgc tgacagttgc gttgttgact
      361 gtgttgctaa tcccgcaggg gatggtaatc catgcagcgg attataacta tggtgaggca
      421 ctccaaaagg caataatgtt ctacgagttt cagagatccg ggaagttacc ggaaaacaca
      481 agaaataact ggcgtggtga ttccgccctt aacgacgggg ctgacaatgg cctggatctt
      541 actggaggtt ggtttgatgc cggagaccat gtaaaattca accttccgtt ggcttatacc
      601 gttaccatgc tggcatggag tgtatatgag tccagggatg cttatgaaaa aagcggacag
      661 cttccataca tacttgacaa cattaaatgg gctacagact acctcataaa atgccatccc
      721 agtccaaatg tttattatta ccaggttgga gactccgcat tagaccattc ctggtgggga
      781 ccggcggaag tactgccgat ggcaaggcca tccttcaaag tggatacgtc aaatcctggt
      841 tcaacagttg ttgctgagac tgcagcggct atggcagctt cttcgattat ttttaagtcc
      901 actgatccgt catactcagc tacacttctt atgcatgcga aagatctgtt tacctttgca
      961 gatactacac ggagcgatac gggatataag gaagcggaag gatattattc atcccacagc
     1021 ggtttctatg atgaactcac ttgggcaagt gcatggctgt atcttgcaac cggagatgat
     1081 gcttacatta aaaaagcaga atcctataaa cccaattggg aaacagagag gggaacggat
     1141 ataataaagt attcgtgggc tcattgttgg gataacaaat ccttcggtgc cttcttactg
     1201 ttagccagaa ttaccgggga ttctttctat aagcagtgta ttgaaagcaa tcttgattgg
     1261 tggaccaccg gacataacgg aactaaggtt caatatacac ccaaaggcct tgcatggctt
     1321 gacggatggg gatcattgag gtatgcaaca acacaggcat ttctggccag tgtttatgct
     1381 gattgggacg gttgtgatcc gtctaaggca tctaaatatc aagagtttgc aaagagtcag
     1441 gtagattatg ctcttggaag tgcaggaaga agcttcgtag taggatttgg aacgaatccg
     1501 cctaaaaatc ctcaccatag gactgcccac ggttcctggt gtgcgatgat ggatacacca
     1561 ccacagagca ggcatgttct tgtcggtgct ttggttggag gacccggaag cagtgacaat
     1621 tacgttgaca gcttgagtga ttatcagtgc aatgaagttg caaatgacta taatgccggt
     1681 tttgtcggtg tgcttgcaaa aatgtatgat aaatatggcg gcacccctat tcctaatttt
     1741 gtagcttatg aaacaccgg
//
LOCUS       DAOOJC010000458         1758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_54443_length_1758_cov_3.756312, whole genome shotgun sequence.
ACCESSION   DAOOJC010000458 DAOOJC010000000
VERSION     DAOOJC010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54443_length_1758_cov_3.756312"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLA01_10185"
     CDS             <1..335
                     /locus_tag="PLA01_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4320 family protein"
                     /protein_id="HPD01701.1"
                     /translation="AIAMVLALIVKVMPVFIAKQQLDTFASELCRTAEITGAVGSATT
                     VKAEQLRNQTGLNPTIQWSRMGNIQLNEEFTVTLTSSVNIGLFGEFASFPITLTSKAT
                     GTSEVYHK"
     gene            332..877
                     /locus_tag="PLA01_10190"
     CDS             332..877
                     /locus_tag="PLA01_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01702.1"
                     /translation="MTKLKKALSNKTGSSTPLIVAVVLAIIILSGAAFEYMRLMIVAV
                     GVRDAVQSAVIDVATENWDEAYNGLREGYSGGYVLSGTSWNQNITTGDIYGRLKDNLG
                     LKQENGKYVKYAGSTLEYAVSGLSVSVTNAPLAPSNINGISQLTVEGTVDIAVPLSFG
                     WEHLPPMQIKMKLKAGYTPKF"
     gene            1036..1605
                     /locus_tag="PLA01_10195"
     CDS             1036..1605
                     /locus_tag="PLA01_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01703.1"
                     /translation="MEKLTPKIKRGFIIAGLSTLCVLLVIGIFAMLEGGTDKTKLPAT
                     LQDADNSVSVGGINNDKTSEPSVTVQEVTPKETQDTNTLKPNDIQLTEIPVRPQPPEK
                     PDTAVDSDKPHDIPKDTNLTNPDKKPTVTVKPVEPTKPKEDKPTGGETNSKGEVYVPG
                     FGWVKNSGSNEEIKSQSDGDWNKQIGDMN"
BASE COUNT          568 a          320 c          403 g          467 t
ORIGIN      
        1 tggcgattgc aatggtcctg gcgcttattg taaaggttat gcctgtattt atcgcaaagc
       61 aacaattaga tacatttgcc tctgaacttt gcaggactgc tgaaattacc ggtgccgtag
      121 gaagtgctac cacagtcaaa gcggaacaac ttcgtaatca aacaggatta aatcctacga
      181 tacaatggtc gagaatgggt aatattcaac tcaatgagga atttaccgtt actttgactt
      241 cttccgtaaa cattgggctg tttggtgaat ttgcttcatt tccaatcaca ctaacatcaa
      301 aagcaacggg cacatcggag gtgtatcata agtgacgaaa ctgaaaaaag ccctgtctaa
      361 taaaacgggc agttctacac ctttaattgt tgcagttgtg cttgctatca ttattttgtc
      421 cggtgctgcc tttgagtata tgaggctgat gatagtggca gtaggtgtaa gagatgctgt
      481 acaatcagca gtaattgatg tagctactga aaattgggat gaagcatata acggtctgcg
      541 ggaaggctac tcaggaggat atgttctgtc agggacatct tggaatcaaa atataacgac
      601 cggtgacatt tacggaaggc tcaaggataa tcttggattg aaacaagaaa atggcaagta
      661 tgtaaagtat gccggcagta cattggaata tgcagtttca gggttatcgg ttagtgtaac
      721 taatgctcct ttggctccgt caaatataaa cggaatcagc caactcaccg ttgagggtac
      781 agttgatata gctgtgcctt tatcttttgg atgggagcat ttaccgccga tgcagataaa
      841 aatgaaactc aaagccggtt atacacctaa gttctgatag agcctatatg atttaggctt
      901 gacaaagcac cgaaaatgat gcgaaacata gtgcataatt attactggac gttacgcaag
      961 tccggtggta taatgtagcc aagaagctgc aaaacttgat agattgattt atgccttaga
     1021 aggaggtatg ctgttatgga aaaattaacc cctaaaatta aaaggggatt tataattgca
     1081 ggtttgtcca ctctttgtgt tctgcttgtt atagggatat ttgcaatgct tgaaggcgga
     1141 acggacaaaa ctaaattacc ggccaccttg caggatgcag ataattctgt atctgtaggc
     1201 ggaattaata acgataaaac aagtgaacca agtgttactg tgcaggaggt tactccgaaa
     1261 gagacacagg acacaaatac attaaaaccc aacgatattc aattgacgga aattcctgtg
     1321 agacctcagc cacctgaaaa gcctgataca gcagtggata gcgacaaacc acacgatatc
     1381 cctaaagata caaacttgac taatccggac aaaaaaccta ctgtaacggt caaaccggta
     1441 gagcctacaa aacccaaaga ggataagccg acgggcggtg aaaccaacag caaaggcgaa
     1501 gtctatgttc ccggatttgg ctgggtcaag aacagtggtt caaatgaaga aatcaaaagc
     1561 caatcggatg gggactggaa taaacagatt ggtgacatga attaactgaa tataggcatt
     1621 taagcagagg taagtcttaa ggatttacct cttttaattt tatgaggagg tcaagatgaa
     1681 aagcaaaata ttttctgtat ttctaatcat cgcccttttg tttacgtctc cgttaacagc
     1741 ttttgcagaa ggagatgg
//
LOCUS       DAOOJC010000459         1733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_55599_length_1733_cov_3.541120, whole genome shotgun sequence.
ACCESSION   DAOOJC010000459 DAOOJC010000000
VERSION     DAOOJC010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55599_length_1733_cov_3.541120"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLA01_10200"
     CDS             <1..336
                     /locus_tag="PLA01_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HPD01704.1"
                     /translation="FQGGVAANVGIVAAFEKAIGKKISIPKYYDVMGAYGAALIAREE
                     MARNGRTTNFYGFESSQKDFKARSIECNGCSNMCEVIEITSDNEVLARWGDRCGKWSA
                     LRSEDQSVS"
     gene            455..1633
                     /locus_tag="PLA01_10205"
     CDS             455..1633
                     /locus_tag="PLA01_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HPD01705.1"
                     /translation="MKDYFVDLHVHIGRSSNGMEIKRATAKNLTFENIAFESYSRKGI
                     DVIGVVDCISPFIIEDIERLAEKGELTEKKGGGMEYRKGQVLILGAELETHEKRGCSA
                     HSLCFFPSLKSIKDFAKDITNHIKNIYSCSSICRLTAQELFDMVDFYGGTYIPAHVFT
                     PHKSFYGNCCDSLLEVFNEKSLEKIPAIELGLSADSMMASQLSELDGKAFLSNSDAHS
                     LMKMGREYNIFQMKEASYEEILKAFKGIDGRRIKANYGLDPKLGKYHRSYCLVCDSVI
                     VGEAPVLKCPASDRHHVVVGVRDRFMLIKDRDKPRMNGRPPYYYQVPLEFLPKVGPKT
                     IDKLINRFGSEMKVLHQATFEELAGATKEDVARNIILSREGKLSIEAGGGGLYGRVEA
                     "
BASE COUNT          539 a          285 c          458 g          451 t
ORIGIN      
        1 tttcagggtg gtgttgctgc caatgtcggt atagtagcgg cttttgaaaa agcaatcggt
       61 aaaaaaatat cgatacccaa atactatgat gtgatgggtg cctatggagc tgcactgata
      121 gcccgggaag aaatggcaag aaacggccgg accacgaact tttacggatt tgagagttca
      181 cagaaagact tcaaagccag aagtattgag tgtaatggat gttcaaatat gtgcgaggtt
      241 atagaaataa catcggataa tgaggtcttg gcccgctggg gtgaccggtg cggaaaatgg
      301 tcggcattaa gaagtgaaga ccagagcgta agttgataat ggaagggtta tggtttatta
      361 ttataagtag cttagacttt gtaaaagaat ccggggttta attatggatg tgatctattg
      421 taaaaaggct gcataaagtt tgagggggac atttatgaag gattattttg tagatcttca
      481 cgttcacata ggaagatcct ctaatggaat ggagataaag cgggctacag ccaaaaatct
      541 taccttcgaa aacattgcat tcgagtcgta ctcaaggaaa ggcatagatg tcattggagt
      601 tgttgactgt atttctcctt ttattattga ggatatagag aggcttgctg aaaaaggtga
      661 gctgactgag aaaaagggcg ggggaatgga ataccgtaaa ggacaggtat taatactggg
      721 agcggagctt gagacccatg aaaaaagggg ctgcagtgct cactccctgt gtttttttcc
      781 ttctttaaag tcgattaagg actttgcaaa agacataaca aaccacataa aaaacatcta
      841 cagttgttct tcaatatgca gattgacagc tcaggagctg tttgacatgg tggactttta
      901 cggaggtaca tatatacctg cccatgtatt cactccccat aagagctttt atggaaattg
      961 ctgcgacagt ttattggaag tattcaacga aaaatccttg gagaaaattc ctgcaattga
     1021 actgggctta agtgccgatt ccatgatggc aagtcagttg agtgagcttg acggcaaggc
     1081 gtttttaagc aattccgacg ctcattcgtt aatgaagatg ggaagggaat acaacatatt
     1141 tcaaatgaaa gaagcgagtt atgaggaaat tctcaaagca tttaaaggga ttgacggcag
     1201 gagaattaag gctaattacg gtttggaccc gaagcttgga aaataccaca gaagttattg
     1261 ccttgtatgc gacagcgtga ttgtggggga agcaccggtt ttaaaatgcc cggcttcaga
     1321 caggcatcat gtggtggtgg gtgtcaggga cagatttatg ttgataaagg acagggacaa
     1381 accacgtatg aatggacggc cgccttacta ttatcaggtt ccactggagt ttttgccgaa
     1441 agtcggacca aagaccatag acaagttgat taaccgtttc ggaagcgaga tgaaggtgtt
     1501 gcatcaggct acatttgagg aattggccgg tgcaacaaaa gaggatgtgg caagaaacat
     1561 cattctttca agggaaggaa agctctctat tgaagccggg ggcggagggc tatacggaag
     1621 agttgaagca tgaaccaaac acaaaaagca ggaaaacatt taaaatatgt tttcctgctt
     1681 tttgctgtgt gccaggcatg gcactaatct agctagtgaa agtctagcca cgg
//
LOCUS       DAOOJC010000460         1705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_57003_length_1705_cov_3.373333, whole genome shotgun sequence.
ACCESSION   DAOOJC010000460 DAOOJC010000000
VERSION     DAOOJC010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57003_length_1705_cov_3.373333"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLA01_10210"
     CDS             <1..186
                     /locus_tag="PLA01_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase"
                     /protein_id="HPD01706.1"
                     /translation="MMITCKNGSKLVEKLNSNGIKATIIGKVTGDGERKLVCGDKVLY
                     IGESSTDELYKTRIDSY"
     gene            337..1575
                     /gene="glyA"
                     /locus_tag="PLA01_10215"
     CDS             337..1575
                     /gene="glyA"
                     /locus_tag="PLA01_10215"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPD01707.1"
                     /translation="MFSLDKLVKFDPEVAKAIEDEVNRQRNNIELIASENFVSEAVMQ
                     AMGTPLTNKYAEGYSGKRYYGGCEYVDVVEDLAIERAKKIFGAEHANVQPHSGAQANM
                     AVFFAVLNPGDTVLGMDLSHGGHLSHGSPVNMSGKYFNVISYGVRKDNFRIDYDEVRK
                     MAKEHKPKIIIAGASAYPRVIDFKAFREIADEVGAYLMVDIAHIAGLVAAGVHPSPVP
                     YAHFVTTTTHKTLRGPRGGMILCSNEHAKMIDKAVFPGIQGGPLMHIIAAKAVSFGEV
                     LTDEFKQYQKQIVKNASVLANALMEKGLEIVSGGTDNHLMLVDLRNKGLTGKYVQNIL
                     DDVRITVNKNGIPFDTQSPFITSGIRIGTPAVTARGMKEEDMLEIADLINLTITDYEN
                     SRDAVRERVSNLCNKYPLYK"
BASE COUNT          559 a          271 c          405 g          470 t
ORIGIN      
        1 atgatgatta cctgtaagaa cggttcgaag cttgtcgaaa agcttaattc caatggaatc
       61 aaagccacaa taatcggtaa agtaaccggc gacggagaaa gaaaactggt ttgcggtgat
      121 aaggttttat atatagggga aagtagtacc gatgaactgt ataaaactcg aattgacagc
      181 tattgattga tgcccgtttc ataataaact ttagttgtat tgagttcaat aaacatctaa
      241 ataatcgttg acatagcgga caattattaa tataatttta ttgtttgatt gggatactca
      301 agtgttatgt tcccttaata attaggaggt ttttttatgt ttagtctaga taaattagta
      361 aaatttgacc ctgaggttgc aaaagcaatt gaggatgagg tgaaccgtca aaggaataat
      421 atagagctta tcgcatcgga gaattttgtt agtgaagccg taatgcaagc gatgggtaca
      481 cccttgacaa acaaatatgc cgaagggtat tcgggaaaaa ggtattatgg tggctgtgaa
      541 tatgttgatg ttgtagaaga tcttgcgatt gaacgtgcaa agaagatttt tggagcagaa
      601 catgcaaatg ttcagcccca ttcaggagca caggctaaca tggcggtatt ttttgcagtc
      661 ctaaatcccg gggatacggt tttaggcatg gatctttccc atggcggaca cttaagccat
      721 ggaagtcctg taaacatgtc aggaaaatat tttaatgtga tatcttatgg agtcagaaag
      781 gacaacttca ggatagatta tgacgaagtc aggaaaatgg caaaggaaca caagccaaaa
      841 ataataatag caggtgcaag tgcatatccg agggtaatag attttaaagc cttcagggaa
      901 attgcagatg aggttggagc atatttaatg gtggatattg cacacatagc agggttggtt
      961 gctgcaggtg ttcatccaag tccggttccc tatgcccatt ttgttaccac caccactcac
     1021 aagactttga gaggaccaag aggcggtatg atattgtgca gcaatgagca tgcgaagatg
     1081 attgacaagg ctgtcttccc cggaattcag ggcggacctc tgatgcatat catagctgca
     1141 aaggctgtta gttttggaga agtgttgact gatgagttta aacaatatca gaaacagata
     1201 gttaaaaacg caagtgtact tgccaatgca ttgatggaaa agggccttga aattgtttcc
     1261 ggcggaacag acaatcattt gatgttggta gatttgagga ataagggact cacaggtaaa
     1321 tacgttcaga acattttgga cgatgtacgt ataacggtaa ataaaaacgg aattcctttt
     1381 gatacgcaaa gcccattcat aacaagcggt attcgcatcg gtacaccggc tgttacggca
     1441 aggggaatga aagaagagga tatgctggag atagcggacc ttatcaatct taccattact
     1501 gattacgaaa actccaggga tgccgtaaga gaaagggtaa gcaatttatg taataaatat
     1561 cctctatata aatagcaatt gaattgtttt aacaataatt ataaaagcat ctaattataa
     1621 aagcaccggc taattaaccg gtgctttatg gtgtgtgcca ggcatggcac taatctagct
     1681 agtgaaagtc tagccacggg tattt
//
LOCUS       DAOOJC010000461         1693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_57631_length_1693_cov_3.354701, whole genome shotgun sequence.
ACCESSION   DAOOJC010000461 DAOOJC010000000
VERSION     DAOOJC010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57631_length_1693_cov_3.354701"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /gene="proC"
                     /locus_tag="PLA01_10220"
     CDS             <1..559
                     /gene="proC"
                     /locus_tag="PLA01_10220"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPD01708.1"
                     /translation="AMDDKKILVSVAVGIPIKFYQGIVGADKRIVRTMPNTPALVGEG
                     MTLMCWENVSDDEGKTVREIFECFGKVEYLDEKLMSEVTALTSSSPAYVFMFIEAMAD
                     AAVLSGIPRNLAYKLAAQAVLGSAKMVLETGKHPGELKDMVCSPAGTTIEAVSSLEKN
                     RFRYAVIEAMNECTRKALEIGKKYD"
     gene            635..1075
                     /gene="rnhA"
                     /locus_tag="PLA01_10225"
     CDS             635..1075
                     /gene="rnhA"
                     /locus_tag="PLA01_10225"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HPD01709.1"
                     /translation="MKKVIIYTDGACSGNPGDGGWGAILVYGKHEKEISGFEKETTNN
                     RMELKAAIEALRLLKEPCEVDLYSDSAYLVNAFVQNWVSNWKSNGWKTKGKDDVKNIE
                     LWKELDRLCDVHRIKWIKVKGHSDNEYNNRCDKLATDEIKKNKQ"
     gene            1105..1653
                     /locus_tag="PLA01_10230"
     CDS             1105..1653
                     /locus_tag="PLA01_10230"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPD01710.1"
                     /translation="MKYEEKTLSKKEFYKGNIINVEKWQVLLPNGKEASRDVVIHPGA
                     SVIIPLTENNEIYMVRQYRKPVEAELLELPAGKLDAGEDPEVCAKRELKEETGLDADE
                     IKHVLSFNSTPGFSNEVLHMYVAAGLHEGVACADEDEFLRIEKVPVKKLLDMISSREI
                     TDAKTIIGILLADRILKGEMII"
BASE COUNT          583 a          248 c          430 g          432 t
ORIGIN      
        1 ggccatggac gataagaaaa ttctggtatc tgttgctgtt gggattccca ttaaatttta
       61 tcagggcata gtaggagcgg ataagaggat tgtgcgtaca atgcccaata ctcctgcact
      121 agtcggagag ggtatgacac ttatgtgctg ggagaatgtt tctgatgatg agggcaaaac
      181 cgtaagggaa atatttgaat gctttggcaa ggttgagtat ttggatgaga agcttatgag
      241 tgaagttaca gcacttacca gcagcagccc ggcatatgtt tttatgttta ttgaggcaat
      301 ggcggatgct gcggttttat cgggaatacc caggaatttg gcctataaac tggctgccca
      361 ggctgtttta ggatcggcca aaatggttct tgaaacggga aaacatccgg gtgagctgaa
      421 agatatggta tgttctcctg cgggtaccac cattgaagca gtgagttctc ttgaaaagaa
      481 ccgattcaga tatgcggtaa tcgaggctat gaatgagtgt accagaaaag ctctggaaat
      541 aggaaaaaaa tatgattaat gaacaatatt atgaatgggt ggatataaac caatacaaaa
      601 catagggcgt aaaatattca attaaggaga tgctatgaaa aaagttatca tatacactga
      661 cggagcttgt tccggcaatc cgggagacgg aggctggggg gctatactgg tgtatggcaa
      721 gcatgaaaag gagatatccg gttttgaaaa ggaaaccacc aataacagga tggaattaaa
      781 ggctgctatt gaagctttaa ggctattaaa ggaaccttgt gaggttgatc tctacagtga
      841 cagtgcatac ctggtaaatg catttgtaca gaactgggta agcaactgga aatccaatgg
      901 atggaaaacc aaagggaaag atgacgtaaa gaatatagaa ttgtggaaag aattagacag
      961 gctttgtgat gttcacagga ttaaatggat aaaggtcaag ggtcacagtg ataatgaata
     1021 caataacagg tgtgacaagc ttgctacaga tgaaattaaa aaaaacaaac agtaatataa
     1081 tatttaatag aggacgggaa taaaatgaaa tatgaagaga aaactttatc taaaaaagag
     1141 ttttataaag gaaatataat taatgtggaa aaatggcaag ttcttttgcc caatggaaaa
     1201 gaagcgtccc gggatgtagt tatccacccg ggagcctccg tcataatacc gttaactgaa
     1261 aacaatgaaa tatatatggt aagacagtac cgaaagcctg ttgaagcgga gcttctggaa
     1321 ttacccgccg gtaaactcga tgcgggagaa gatcctgaag tttgtgccaa aagggagttg
     1381 aaggaggaaa cgggcttgga tgccgatgag ataaagcatg ttctgagttt taacagcacc
     1441 ccgggctttt caaatgaagt gttgcacatg tatgtggctg ccggacttca tgagggtgtt
     1501 gcgtgtgccg atgaagatga gttcttaaga attgaaaaag tacctgtaaa aaagctttta
     1561 gatatgattt caagccgtga aataactgat gcaaaaacca ttatcggaat actcctggca
     1621 gatagaatat taaaggggga aatgataata taaatatcaa agagttataa ttttttagta
     1681 aagtttacaa tac
//
LOCUS       DAOOJC010000462         1687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_57944_length_1687_cov_3.839461, whole genome shotgun sequence.
ACCESSION   DAOOJC010000462 DAOOJC010000000
VERSION     DAOOJC010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57944_length_1687_cov_3.839461"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..893
                     /gene="murB"
                     /locus_tag="PLA01_10235"
     CDS             3..893
                     /gene="murB"
                     /locus_tag="PLA01_10235"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPD01711.1"
                     /translation="MLEGIVGSGNIKVDEPMKMHTSFRVGGPADILVTPVSVSQLSEI
                     LKLCKRENVPILVMGNGTNLIVSDNGIRGVVIKIYDNMNGCSVENDTIRAYGGILLSK
                     LSSVALENGLTGLEFASGIPGTLGGAVAMNAGAYGGEMKDIVFETEYMDKDGELKVLR
                     NEEHEFGYRTSFIQKQSGMVIRSVLKLRKGNKDDIKALMRDLTKRRQAKQPLDMPSAG
                     SIFKRPEGYFAGKLIEDSGLRGYQIGGAQVSDKHCGFIVNTGNATARDIINLIKHVQA
                     TVKMKFGVEMQTEVKIIGED"
     gene            933..>1687
                     /gene="rapZ"
                     /locus_tag="PLA01_10240"
     CDS             933..>1687
                     /gene="rapZ"
                     /locus_tag="PLA01_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPD01712.1"
                     /translation="MRLLIITGISGAGKSLVVKYLEDIGFFCVDNLPPLLIRKFVEIC
                     IQSRGKINKIALVIDIRGGELFNDLVPELNDLKEAGISYEILFLEASDNVLVKRFKES
                     RRIHPLATGGRLIEGIKKERETLSEIRKNATHVIDTSNLTPRQLKEEITGIFLEGKRF
                     DGMIINIISFGFKYGIPIDCDLVFDVRFIPNPFYIDSMKKQTGKNEAVREYVLGFKET
                     SEFLVKLKDMVEFLIPNYIKEGKSQLVIGIGCTG"
BASE COUNT          550 a          254 c          428 g          455 t
ORIGIN      
        1 cgctgttgga aggcattgtt ggttccggta atataaaggt tgacgaacct atgaagatgc
       61 atacttcatt tagagtggga ggacctgcgg atattctggt tactcctgtg tcggtttctc
      121 aactgagcga aattttaaaa ctttgcaaaa gagaaaatgt accgatactt gttatgggga
      181 acggtacaaa tcttatcgtg tccgataatg gtatccgggg agtggttatt aaaatatatg
      241 acaacatgaa cggttgcagc gttgaaaacg acactatccg ggcatacggc ggcatattgc
      301 tttcaaagct gtcgagtgtg gctcttgaaa atggtctcac cgggctggag tttgcctccg
      361 gtataccggg aacattaggc ggtgctgttg caatgaatgc cggtgcctat ggcggtgaga
      421 tgaaagatat tgtatttgag accgagtaca tggataagga tggcgagctt aaggtattac
      481 gtaatgaaga acatgaattc ggctacagaa ccagctttat tcaaaaacag tcgggtatgg
      541 taataagatc ggtgttgaaa ttgagaaaag gtaataaaga tgatataaag gcattaatga
      601 gagatctgac aaaaaggaga caggcaaaac aacctctgga tatgcccagt gcaggcagca
      661 tttttaaaag accggagggg tattttgcag gaaaactcat cgaggatagc ggacttagag
      721 gctatcagat tgggggtgct caggtttcag acaaacattg cgggtttata gtaaataccg
      781 ggaatgcgac agcccgggat ataataaacc tgataaaaca tgttcaagca acggtaaaaa
      841 tgaaatttgg agttgaaatg caaacagaag ttaaaataat tggcgaagat tagacaatat
      901 atatatatat agataaaaaa caggaggcac aaatgaggtt actgataatt accggaattt
      961 caggggctgg gaagagtctt gttgtaaagt atttggagga tatagggttt ttctgtgtgg
     1021 ataatcttcc tcctttactc atacgcaaat ttgtagaaat atgtatacaa agcaggggga
     1081 aaataaataa aattgctctt gttatcgata taagaggcgg agaattattt aatgatctgg
     1141 ttccggaact caatgattta aaagaggctg gaatatccta tgaaattctg tttttggaag
     1201 cgtcggacaa cgtactcgtc aagaggttta aagagagcag gcgcattcac cctcttgcaa
     1261 cgggaggaag actgatagag ggaattaaaa aggaaaggga gacattatcg gaaatcagga
     1321 aaaatgcaac acatgtgatt gatacttcca atcttacacc aaggcagctt aaggaggaaa
     1381 tcacagggat ttttctggaa ggcaagcggt ttgatggaat gataataaat atcatttcct
     1441 ttggatttaa gtatggtatt cccatcgact gtgacctggt ttttgatgtg agatttattc
     1501 ccaatccctt ttatattgac agtatgaaga aacaaacagg caagaacgag gcggtaagag
     1561 aatatgttct tggctttaaa gaaacctccg agtttcttgt caagctgaaa gatatggtgg
     1621 agtttttgat tccgaactat ataaaagaag gcaagtcaca gcttgttatc ggaattggtt
     1681 gtaccgg
//
LOCUS       DAOOJC010000463         1677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_58468_length_1677_cov_4.115906, whole genome shotgun sequence.
ACCESSION   DAOOJC010000463 DAOOJC010000000
VERSION     DAOOJC010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58468_length_1677_cov_4.115906"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PLA01_10245"
     CDS             <1..651
                     /locus_tag="PLA01_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPD01713.1"
                     /translation="VDVSGAGSSTIRIRGKNKRLKNVEHTVIPDRIVAGTYLAAAGIT
                     GGDLNLRNVSAEHIMSVVTYLREAGCRVNIKKNNIHVSGPARPRAIDIIRTLPYPGFP
                     TDMQSQFVSLLSVARGTSIIVETIFENRYKHVEELLRMGADIKLEGRLAVIKGVKMLT
                     GAPVVARDLRGGAALVLAALAAEGTTVINGTKHIDRGYEDIEKKLSMVGAFIKREQ"
     gene            672..1523
                     /locus_tag="PLA01_10250"
     CDS             672..1523
                     /locus_tag="PLA01_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPD01714.1"
                     /translation="MQKITTGAEYQPKKKHKRKKSGIFRFVRFILAALFIVAVLVSLA
                     LSPLFNINRIEVYGNKHYNSNDVINASGLIAGTNWFKSNSFDLKGIVMFRSVDSEKML
                     LERCPYLKSAVVRIGIPGAVRITVTEREPVALVPYLGTNLVIDDNGHVIDTTNNIDGI
                     KLPVIKGVACNGYTLGQALKADNYEYIDAFNTVMKNIASSDANAGERGKEFCIEDIIT
                     YIDVTDLDNVGVCLDSRIVVNFGSYKEINEYRIDFLREIYYQKLKKEDRGLLDFTSGE
                     YPSFIPD"
     gene            1539..>1677
                     /locus_tag="PLA01_10255"
     CDS             1539..>1677
                     /locus_tag="PLA01_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1290 domain-containing protein"
                     /protein_id="HPD01715.1"
                     /translation="MIPLLGLIIGIVIGIFVPYNIPDQYSSYVAVAILAALDSVFGGV
                     VS"
BASE COUNT          509 a          274 c          421 g          473 t
ORIGIN      
        1 gttgatgttt caggagccgg ttcgagcact ataaggataa gaggcaaaaa caagaggcta
       61 aagaatgttg aacataccgt cattcccgac agaattgtag caggtacata tttggcggcc
      121 gcagggatta ccggaggaga tttaaacctt agaaacgttt cggcagaaca tattatgtcc
      181 gttgtaactt acctgagaga agccggctgc cgtgtcaata taaagaaaaa caacatacat
      241 gtttccggtc ctgccagacc ccgtgctata gacataatca gaaccctgcc ctatcctggg
      301 tttcctaccg atatgcagtc ccagtttgta tcactgcttt cggttgcccg gggaaccagt
      361 ataatagttg agacaatatt tgaaaataga tacaagcatg ttgaagagct tctaagaatg
      421 ggagcagata taaagcttga aggcaggttg gcagtaatta aaggggtaaa gatgctgacg
      481 ggtgcccccg tagtagcgag ggatttgaga ggcggggctg ctttggtgct tgcggcgtta
      541 gcagccgagg gcactactgt tataaacggt acaaagcata ttgaccgagg gtacgaagac
      601 attgaaaaaa agctgtccat ggttggggct tttattaaaa gagaacaata aaggggtaag
      661 gtctgttgaa agtgcaaaag attacaaccg gtgctgagta tcagccgaaa aaaaagcaca
      721 aaagaaaaaa gtccgggatt tttcgttttg tcaggtttat attggctgcc ttgtttattg
      781 tggctgtctt ggtttctttg gcactttctc ctttatttaa tatcaacagg attgaagttt
      841 atggcaataa gcattataat agcaatgatg tgattaacgc atccggtttg attgcgggaa
      901 ccaactggtt taaatccaac agctttgatt taaagggaat agttatgttc aggtcagttg
      961 attccgagaa aatgctattg gaaaggtgcc cctatttaaa aagtgctgtt gtaagaatag
     1021 gaataccggg tgcggttaga attaccgtaa ccgaaaggga accggtggcg ttagttccat
     1081 accttggtac gaatcttgtt attgacgata acggtcatgt gatagacaca acaaataata
     1141 tagacgggat caagctgccc gttatcaagg gtgtggcctg caacggttat acactgggac
     1201 aggctttaaa agcggataat tacgagtata ttgatgcttt taatacggtt atgaagaaca
     1261 tcgcttcttc ggatgccaat gccggagaga ggggaaagga attttgcatc gaggatataa
     1321 taacctatat tgatgttacg gatttggata atgtaggtgt ctgccttgat tcgaggatag
     1381 ttgttaattt cggaagctat aaagaaatca acgaatacag gattgatttt ttaagagaga
     1441 tttattatca gaaattaaaa aaagaagaca gaggattatt ggattttaca tccggtgaat
     1501 accccagctt cataccagat tagaaaggga ggataaatat gattccttta ttaggcttaa
     1561 taattggaat tgtgattggg atttttgttc catacaacat tcccgatcag tattcgagtt
     1621 atgttgcggt ggcgatactt gctgcattgg attctgtctt tggaggagtg gtttcaa
//
LOCUS       DAOOJC010000464         1658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_59455_length_1658_cov_3.243918, whole genome shotgun sequence.
ACCESSION   DAOOJC010000464 DAOOJC010000000
VERSION     DAOOJC010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59455_length_1658_cov_3.243918"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PLA01_10260"
     CDS             <1..490
                     /locus_tag="PLA01_10260"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860814.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPD01716.1"
                     /translation="LGYSMESGIPYGVGLIKNRYVGRTFIKPEQGQREDSVRIKLNAL
                     KEAINGKRVVMIDDSIVRGTTSKRIVQMLRNAGALEVHMRVSSPPYKFPCYFGVDVSS
                     KSELVAAKHSIEEIREMIGADSLGFLSLEGLLQTPVGAKCGFCTACFTGDYPMEIPER
                     VK"
     gene            568..1590
                     /gene="purM"
                     /locus_tag="PLA01_10265"
     CDS             568..1590
                     /gene="purM"
                     /locus_tag="PLA01_10265"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPD01717.1"
                     /translation="MTTYKDAGVDVEAGYEAVKLMKNDVRRTFRPEVVTDIGGFGGLF
                     ALNREKYSEPVLVSGTDGVGTKLKIAFLMDKHDTIGIDCVAMCVNDIVCSGAEPLFFL
                     DYIALGKNRPEKVAQIVKGVSDGCVEAGCALIGGETAEMPGFYPEDEYDVAGFAVGIV
                     DKKDIIDGKKIKAGDKIIGLASSGIHSNGYSLVRKVLAPSKEKLSEVVSSLGTTIGEE
                     IIKPTRIYVKTILDLKNRFEIKGVAHITGGGFIENMPRMLPEGLGINIFRGSWPVLPI
                     FNILQSIGNIDERNMFNTFNMGIGMVAAVGADIAQDVLKHLNSSKEQAYLIGEVVSGR
                     NGLELC"
     gene            1584..>1658
                     /locus_tag="PLA01_10270"
     CDS             1584..>1658
                     /locus_tag="PLA01_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPD01718.1"
                     /translation="MLKIGVLVSGGGTNLQAIIDKIENG"
BASE COUNT          501 a          240 c          454 g          463 t
ORIGIN      
        1 cctcggatat tctatggaat ccggcatacc ctatggagtc ggacttatta agaacagata
       61 tgtcggtaga acgtttatca agccggaaca gggacaaagg gaggatagtg tcagaataaa
      121 actcaatgcc ttaaaagaag ccattaacgg aaaaagggtt gtaatgatag atgactcaat
      181 tgttagagga acaacgagta agagaattgt tcagatgtta agaaatgcgg gagctttaga
      241 ggttcatatg agagtcagtt ctccacctta taagttccca tgctattttg gtgtggatgt
      301 atccagtaag tcagagcttg ttgctgcaaa acatagcata gaagagatta gagaaatgat
      361 tggtgcagac agcttgggtt tcttaagcct tgagggtttg cttcaaacgc cggtgggagc
      421 aaaatgcggc ttttgtaccg catgttttac gggggattat ccaatggaaa taccggagcg
      481 ggtaaagtaa gatttccagt gggcttcatt tgccaaattt tattatttgt tatataattt
      541 atttatttgt agtcgggagg tagtataatg accacgtata aagatgcggg tgttgatgtt
      601 gaagcaggtt atgaagctgt aaagctgatg aaaaatgatg tgagaaggac tttcagaccg
      661 gaggttgtta ccgacatcgg aggtttcgga ggactttttg cattgaacag ggagaagtat
      721 tccgagcctg tacttgtatc tgggactgat ggcgtcggca caaaacttaa aattgctttc
      781 ttgatggaca agcatgacac tattggaata gattgtgttg caatgtgtgt aaatgatatt
      841 gtatgcagcg gtgcagaacc tctgtttttt ctggactata ttgcactggg gaagaaccgt
      901 ccggaaaagg ttgcccaaat tgtaaaaggg gtttctgacg gctgtgttga ggcaggatgt
      961 gcattgattg gcggggaaac agctgaaatg ccgggctttt atcctgagga tgagtatgat
     1021 gtggcaggtt ttgcagttgg tattgtagac aaaaaggata ttatcgacgg aaaaaaaata
     1081 aaggcagggg acaaaataat aggtcttgct tccagcggta ttcacagcaa cggatattct
     1141 cttgtaagga aggttttggc acctagtaaa gaaaaactgt cggaagttgt ttcgtctctg
     1201 ggaaccacaa ttggagaaga gataataaag ccgacaagga tatatgttaa gacaattttg
     1261 gatttaaaaa acaggtttga aataaaaggt gtagcccata ttaccggtgg aggttttatt
     1321 gagaatatgc caaggatgct gccggagggt ctgggaataa acatattcag aggttcctgg
     1381 ccggtattgc cgatatttaa tattcttcaa agcatcggga atattgatga aagaaatatg
     1441 ttcaacacct ttaatatggg tataggtatg gtggcggcag ttggtgcgga tattgcacag
     1501 gatgtgctta agcacttgaa cagcagtaaa gagcaggctt atttaatagg agaagttgtt
     1561 tcaggaagga acgggctgga gctatgctga aaatcggtgt tctggtttcc ggaggaggaa
     1621 ccaatctgca ggctattata gataagattg aaaacggt
//
LOCUS       DAOOJC010000465         1626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_61245_length_1626_cov_2.887969, whole genome shotgun sequence.
ACCESSION   DAOOJC010000465 DAOOJC010000000
VERSION     DAOOJC010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61245_length_1626_cov_2.887969"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLA01_10275"
     CDS             complement(<1..250)
                     /locus_tag="PLA01_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPD01719.1"
                     /translation="MTLQEMKKKLEETLSSKRFKHSIGVMDTAVKLAKKYDEDEDKAA
                     IAGILHDCARNIEGQKVFEFCERYGIEVNYITRLQPQLL"
     gene            complement(284..907)
                     /gene="nadD"
                     /locus_tag="PLA01_10280"
     CDS             complement(284..907)
                     /gene="nadD"
                     /locus_tag="PLA01_10280"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HPD01720.1"
                     /translation="MGNSEKRVGILGGTFDPIHIGHLIIAEQIREEFQLDKVIFIPSG
                     NPPHKKLQTVTDAEHRYNMVCEALKGNPYFEVSRIEIDRKGYTYTIDTLNDLKEQNQE
                     NAHMYYIIGADVLYDLLTWKDYTKVFEVCEFIAALRPGKDRDGFRERIRYLEKNFSAK
                     IHEADISLIEVSSTMVRERVKQNRSVKYLVPEAVEDYIKRNRLYRDN"
     gene            complement(1048..>1626)
                     /locus_tag="PLA01_10285"
     CDS             complement(1048..>1626)
                     /locus_tag="PLA01_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPD01721.1"
                     /translation="NKENVIGIFVGTGIGGGLIINNKLFSGPRHLAGELGHMVLNTEG
                     PFCNCGQRGCLEAYSSKVAITKEIRVQIERGRDTVLKDLMEDDSIIKSKALKKALEQK
                     DPVALEVMDRAVYYLAAGTGSLINIFNPDMVVLGGGVLEALGEYIIPRLNKHIKRFAL
                     PAMLEGTDIVESMLGDDAILYGALALIKDRVT"
BASE COUNT          446 a          414 c          247 g          519 t
ORIGIN      
        1 gcagaagttg cggctgtaat cttgttatat aatttacttc tattccatac ctctcgcaaa
       61 actcaaacac tttttgcccc tcaatattcc ttgcacaatc atgcaaaata ccggcaattg
      121 ctgccttatc ctcatcctcg tcgtattttt tagccaactt aacagctgta tccatgaccc
      181 ctattgagtg tttaaatctc ttcgaagaaa gggtttcttc aagctttttt ttcatttctt
      241 gtaatgtcat tgctgttacc cctgtaaata actttattta ctatcaatta tcccggtaga
      301 gccggtttct ttttatataa tcttcaacag cttcgggaac aagatatttt acggatctgt
      361 tttgctttac cctttccctt accatagttg aagatacttc tataagggaa atatcagcct
      421 catgtatttt cgcagaaaag tttttctcaa gatacctgat ccgttctctg aacccatccc
      481 tgtccttccc cggccttaat gccgctataa actcacatac ctcaaacacc ttcgtgtaat
      541 ccttccatgt gagaaggtca tagagtacat cagcaccaat tatatagtac atatgagcat
      601 tttcttggtt ttgttccttt aaatcgttca gggtatctat ggtataagta taccccttcc
      661 tgtcaatctc aatccttgac acctcaaaat acggatttcc cttcaatgcc tcacatacca
      721 tattgtatct gtgctcggca tcggttacag tttgcaactt tttatgggga ggattgccag
      781 agggtataaa tatcacctta tccagttgaa attcctcccg tatctgctca gctataatca
      841 aatgaccgat atgaatagga tcaaaggtac cgcccagtat acctactctc ttttcactgt
      901 ttcccatctt caataccttc actaaaatat taaatacatc ttccggaaaa taattatagc
      961 ataaaatccc tgttcttaaa agccgcacta attttttagg gtgcacatta ttcaagcgaa
     1021 aaagatggaa gaaaagtata aaagaaatta agtcaccctg tctttaataa gtgccaaagc
     1081 cccatacaaa atggcatcat ccccaagcat gctttctaca atatccgttc cctcaagcat
     1141 cgccggaagg gcaaaccttt ttatgtgctt attcaaccgg ggtataatgt attctcccaa
     1201 tgcctccaaa acaccgcctc ccaacacaac catgtcagga ttgaagatat ttataagaga
     1261 accggtcccg gctgccagat aatatactgc cctgtccata acctccaggg caacaggatc
     1321 cttttgctcc aaagctttct taagagcttt gctttttatt atcgaatcat cctccatcaa
     1381 atccttcaaa accgtatctc tgcctctttc tatctgcacc ctgatttctt ttgtaatggc
     1441 aacctttgag gaataggcct caagacatcc tctttgtccg cagttgcaga aaggtccttc
     1501 ggtgttcagc accatatgcc ccagctctcc tgcaaggtga cgcggtcccg aaaaaagctt
     1561 gttgtttata ataagtccgc cgccaatgcc tgtccccaca aatataccta tcacgttctc
     1621 cttatt
//
LOCUS       DAOOJC010000466         1578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_64044_length_1578_cov_4.124754, whole genome shotgun sequence.
ACCESSION   DAOOJC010000466 DAOOJC010000000
VERSION     DAOOJC010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64044_length_1578_cov_4.124754"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /gene="tsaE"
                     /locus_tag="PLA01_10290"
     CDS             complement(<1..247)
                     /gene="tsaE"
                     /locus_tag="PLA01_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPD01722.1"
                     /translation="MIEIRTFSQEETIEAGRKLGRLLQKGDIVCITGDLGTGKTILTK
                     GIAKAIGIGEHITSPTFTIVNEYEEGTIPFYHFDVYRI"
     gene            complement(234..1436)
                     /locus_tag="PLA01_10295"
     CDS             complement(234..1436)
                     /locus_tag="PLA01_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPD01723.1"
                     /translation="MLLIKNGRIITMTGTDYENGYILIDEGKIVEVGEYPSGLKKEIL
                     KSDKLSIIDAKNKYVLPGLVDAHCHVGMWEDAVGFEGDDGNEMTDPVTPHLRAIDAVY
                     HLDRAFVEAYQNGVTTVVTGPGSANVIGGQFAALKTYGRRIEDMVVKSPVAIKVAFGE
                     NPKVVYNERKMAPTTRMATAAILRESLLKALEYKGMLDEYNENKEENDKPDYDIKMEA
                     LLGVVNKEIPVKAHAHRADDILTAIRIAKEFDLKMTIEHCTEGHLIADILSDEGIPAI
                     VGPYLTDRSKIELRNLSLTTPGVLSKAGVKVAIMSDHPCMPVQYLALAAAMAVREGMD
                     EAEALKAITINAAEITGIDDRVGSIEPGKDADIAIFDGHPFEIRSKVVNTIINGKVVY
                     ERNKNDRD"
BASE COUNT          395 a          402 c          285 g          496 t
ORIGIN      
        1 aaatcctgta tacatcaaag tgatagaagg gtatcgtgcc ttcttcatat tcatttacta
       61 tggtaaacgt agggcttgtt atatgctcac caatgcctat ggcttttgca attcccttcg
      121 tcagtatggt cttacccgta cccaggtctc ctgtaataca aacaatatca cccttttgga
      181 gaagccttcc cagcttcctt cccgcctcaa ttgtctcttc ctgtgaaaac gttctaatct
      241 ctatcatttt tattcctttc gtaaaccact ttgccgttga taattgtgtt aactacttta
      301 gaccttattt caaaaggatg tccgtcaaat atggcaatat ccgcatcttt acccggttca
      361 atacttccca cccggtcatc tatgcctgtg atctcagcgg catttatcgt aatggcctta
      421 agtgcctcgg cttcgtccat gccttctctc accgccatgg cagcagccaa tgccaggtat
      481 tgaacaggca tacatggatg atccgacata atcgccacct tcaccccagc ctttgaaaga
      541 actccgggag tagtgagact taagttcctg agctcgattt tagacctgtc cgtaaggtag
      601 gggccgacta ttgccggaat cccctcatcg cttaaaatat cggcaataag gtgtccttca
      661 gtgcagtgct cgatggtcat tttcagatca aactcttttg ctatcctgat agccgtcaga
      721 atatcatccg ccctgtgtgc atgtgcctta acgggtattt ctttattgac aacccccaaa
      781 agtgcctcca ttttaatatc ataatcgggc ttgtcgtttt cttccttgtt ctcgttgtat
      841 tcatccagca ttcccttata ttccaaggcc ttaagcaagc tttcccttaa aatagcggct
      901 gtagccatcc gggtagtagg agccatcttt ctttcgttgt acacaacctt ggggttttca
      961 ccgaaagcaa cctttattgc cacaggactt tttacgacca tgtcctctat tctcctgcca
     1021 taggtcttta gtgccgcaaa ctgccctccg atgacattgg cactgccggg accggtaaca
     1081 acggttgtaa caccgttttg ataggcctca acaaaggcac ggtccagatg gtaaacagca
     1141 tctattgcac gaaggtgcgg agtaacgggg tctgtcattt cattgccgtc atccccttca
     1201 aaccccactg cgtcttccca cattccgaca tggcagtggg catcaactag tccgggaagt
     1261 acatatttat tttttgcatc aatgatgctc aatttgtccg atttcaggat ttcctttttc
     1321 agtccggaag gatattcacc cacctcaact atttttccct catcgattaa aatataaccg
     1381 ttttcatagt cagtaccggt cattgtaatt attctaccgt tttttataag cagcatttcg
     1441 acctccaaaa agataaatat tattccacaa agcatatttt attatattgt ttcattgatt
     1501 acaataaaga aaaagtgcag tggcacggct aacgcatgaa tattatctcc aaccaagaat
     1561 tctgtaaaga atttttaa
//
LOCUS       DAOOJC010000467         1566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_64727_length_1566_cov_4.317009, whole genome shotgun sequence.
ACCESSION   DAOOJC010000467 DAOOJC010000000
VERSION     DAOOJC010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64727_length_1566_cov_4.317009"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PLA01_10300"
     CDS             complement(<1..637)
                     /locus_tag="PLA01_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HPD01724.1"
                     /translation="MKGKLIIIESGSDASGKATQTEKLYNRLLQDDYRVRKISFPNYD
                     SDSSALIKMYLSGDFGKNPEDVSPYVASTFYAVDRFASFKTDWGQAYNDGAIILADRY
                     TTSNMVHQAAKIKERGEKDSFLEWLLDFEFNIFKLPEPDCVLFLDMPPEYSKMLMEGR
                     ANKFTGEQKKDIHEINEGYLTESYNNSLYIAKKYGWIMIDCVKDGVLRTIDE"
     gene            complement(797..>1566)
                     /locus_tag="PLA01_10305"
     CDS             complement(797..>1566)
                     /locus_tag="PLA01_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HPD01725.1"
                     /translation="AHKTLPAFTQGSYLHVRGTRTDIKRLKFNLSILQTSSPSYIIMA
                     FLDIARAIMQNEGENRIQNLLREIELLSEIVGKGNVYKILSENDIEGGSIDKTRVVVN
                     VRNTGKTGFEIEKLLRNQYNIQVEMSDLYNIVCIATVADTHEDIKKLGTALDQLRVSF
                     KKAALLKDAPLWIPKLPEQRIDLGSVMNRAFTRKKLTDALQCVSRSMITPYPPGIPVI
                     CPGEVISKDAVEYIREIIEAGGAVNGISEDLEIDVVE"
BASE COUNT          418 a          390 c          259 g          499 t
ORIGIN      
        1 tctcatctat agtccttaaa accccgtcct ttacacaatc aatcataatc cagccgtact
       61 ttttggcaat gtacagtgaa ttattatagg actccgtcaa atacccttca tttatttcat
      121 gtatatcctt tttttgttct ccggtgaatt tgtttgccct gccctccata agcattttgc
      181 tgtattcggg aggcatatca aggaacaata cacagtccgg ttcgggaagc ttgaaaatat
      241 taaactcaaa atccaagagc cactcaagga atgagtcctt ttccccacgt tctttaatct
      301 ttgccgcttg atgtaccatg ttcgacgtag tatacctgtc ggctaaaatt atggccccat
      361 cattatatgc ctgcccccag tcggttttaa aggatgcaaa cctgtctact gcatagaagg
      421 ttgatgccac ataaggactg acatcctcag gatttttccc gaagtctccg cttaaataca
      481 tctttatcag agcagaagaa tcactgtcat agttcggaaa actaattttc cttaccctgt
      541 aatcatcctg caataaccga ttatacaatt tttccgtctg tgttgccttg ccgctggcat
      601 cagaaccgct ctcaataatt atcagcttgc ccttcataaa gcatctcctt tttaaacctg
      661 tatactatcc gaatcccgga atcctgccct ctaattcgaa cctgcacgaa atccgaatct
      721 aaagcttttt ttatccaagt ttcatggaac taaaatatct gtaccaacca cctatgttta
      781 gcttcggcca aacagtttac tcaacaacat ctatttcaag atcctccgat atcccgttta
      841 ccgcaccgcc agcctcaatt atttctctga tatactccac cgcatctttt gaaataacct
      901 caccgggaca tattaccggt attccgggcg gataaggggt tatcatcgat ctgcttacac
      961 actgcagtgc atctgtaagc ttttttcttg taaatgcccg gttcataaca ctgccaaggt
     1021 caatcctctg ctcgggaagc ttcggaatcc acaagggagc atctttcaac agagcggctt
     1081 tcttaaagct gacacgcaac tgatccaggg cagttccaag cttttttata tcttcatgag
     1141 tgtctgccac cgtagcaata cacacaatat tatataaatc agacatttcc acctgtatat
     1201 tatattgatt tcttaaaagc ttttcaattt caaaaccggt ttttcccgtg tttcttacat
     1261 taaccacaac cctggtttta tcaatactgc cgccttctat gtcattttcc gaaagaattt
     1321 tatatacatt gcccttaccg acaatttcgc taagcagttc aatttccctt agaaggtttt
     1381 ggattctgtt ctctccctca ttctgcataa tagcacgggc aatatcgaga aaggccataa
     1441 ttatgtatga cggactggag gtttgaagta ttgatagatt aaacttcagc ctttttatgt
     1501 ctgtccttgt tcccctgaca tgaagatatg acccttgggt aaaggccgga agggtcttgt
     1561 gtgcac
//
LOCUS       DAOOJC010000468         1565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_64791_length_1565_cov_3.535762, whole genome shotgun sequence.
ACCESSION   DAOOJC010000468 DAOOJC010000000
VERSION     DAOOJC010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64791_length_1565_cov_3.535762"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..824)
                     /locus_tag="PLA01_10310"
     CDS             complement(135..824)
                     /locus_tag="PLA01_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD01726.1"
                     /translation="MNYVWDITINLRQAGRDEKDVKFIAAKSYSPYMELSQTEINFDR
                     IEKEIEVNPYYRFYEIFKDLFNINNQEDMELRETLFDILMHFLSDIDTLQGMTKREFY
                     IRFCLRDIANGVLGSGIKKGFEIFNYKEKVIIAENLIKLYKTGQMLYLLKDTVRKIFK
                     YSKIYANYETKDELLFYIPYARSSVNEEKMNFILDFFLPVKFRTEIYWKDHFGIIGVE
                     ESMIIDSIALY"
     gene            complement(821..>1565)
                     /locus_tag="PLA01_10315"
     CDS             complement(821..>1565)
                     /locus_tag="PLA01_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="normocyte-binding protein"
                     /protein_id="HPD01727.1"
                     /translation="YKFFDVLLVECESSIDENEEIKEIVFDLEDLEKHKMIDVVPLWN
                     VKGLRLKSDGFPMPAIDRVNFEHFISTKKMGSANGYLVDEREHAIRYIRREEEGLTIV
                     SPEEKAGTWDILLVSQQKDEDIGNYEMEVSNNTRKESFINKYANRQALIIRTKGEIAR
                     IVNSFEASKAFELCDVEIKPAGNSHGITYDMNFFISDDIRVGNDKKVMVLRFRANTKV
                     GFITQDVLSFLVSEIQMYFPEYECEGELV"
BASE COUNT          459 a          364 c          206 g          536 t
ORIGIN      
        1 aaagtcggca gttcctcaag ctctgctctt gtataaagct taatgctgct gtcttccgta
       61 tggaataatc tatatgaaag catctttata tttctccccc tcttttataa acatactttt
      121 caatcagatt gcgattaata tagagctata ctgtcaatta tcatcgattc ctcaacacca
      181 attatcccaa aatgatcttt ccaatatatt tcagtcctaa acttgacagg cagaaaaaaa
      241 tccaaaataa aattcatttt ctcttcatta acactgctcc ttgcataggg tatatagaaa
      301 agcagctcat ccttggtctc atagtttgca taaatctttg aatatttgaa aatctttcta
      361 accgtatcct taagcaaata aagcatttgt cctgttttat acaacttaat aaggttttct
      421 gcaattatta ctttctcttt ataattaaaa atctcaaacc ccttctttat tccgcttccc
      481 agcactccgt ttgcaatgtc tctaagacag aaccttatgt aaaactctct tttagtcatt
      541 ccctgaagag tgtctatatc agataagaaa tgcattaata tatcaaaaag ggtttcccta
      601 agttccatat cctcttgatt gtttatgttg aacaggtcct taaatatttc ataaaaccga
      661 taatagggat taacctctat ttccttttct atcctatcga agtttatttc cgtttggcta
      721 agttccatgt agggagaata gctttttgca gcaataaatt ttacatcctt ttcatcccgc
      781 cctgcctgcc gtaaatttat cgtaatgtcc cagacataat tcataccaat tccccttcac
      841 attcatattc agggaaatac atttgtatct ccgacaccag aaagcttaag acgtcttgag
      901 taataaagcc cacctttgta tttgccctga acctcaagac cataaccttc ttatcattgc
      961 caacccttat gtcatcggat ataaaaaagt tcatatcata ggttatgcca tgggaatttc
     1021 ctgccggctt aatctcaaca tcacataact caaaagcctt tgaggcttca aaagaattta
     1081 ctattcttgc aatctcgccc tttgtcctta taatcaatgc ctgccggttt gcgtatttat
     1141 ttataaagct ttccttccgg gtattgttgg atacctccat ctcatagttt ccaatgtcct
     1201 catctttttg ttgggaaacc aaaagaatat cccatgtccc ggccttttcc tcgggtgaca
     1261 caatggtcaa cccttcctcc tcacgtctta tgtaccttat ggcatgttca cgttcatcca
     1321 caaggtatcc gtttgcggat cccatttttt ttgtagaaat aaagtgctca aaattaactc
     1381 tatcgatagc aggcatcgga aaaccatcgc tttttagcct caaacctttc acattccaca
     1441 aaggaacaac atcaatcatc ttgtgctttt caaggtcttc cagatcaaag acaatttctt
     1501 taatctcttc gttctcatct attgaggatt cacattcaac cagcaaaaca tcaaaaaact
     1561 tatat
//
LOCUS       DAOOJC010000469         1542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_66302_length_1542_cov_3.625420, whole genome shotgun sequence.
ACCESSION   DAOOJC010000469 DAOOJC010000000
VERSION     DAOOJC010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66302_length_1542_cov_3.625420"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..896)
                     /gene="rsmI"
                     /locus_tag="PLA01_10320"
     CDS             complement(51..896)
                     /gene="rsmI"
                     /locus_tag="PLA01_10320"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPD01728.1"
                     /translation="MDNKGILYLVATPIGNLEDITLRALRVLKEVDFIAAEDTRQTLK
                     LLNHFDIKKPLVSYYEHNKIVKGNYLVGQLLDGKSIALVSDAGTPGISDPGEDIVKLC
                     IENDIQVTMVPGPVAAVTGLVLSGLSAGRFVFEGFLPMNKRQRKDRINSLRDETRTMV
                     LYEAPHKLIYTLKDLYETLGDRRITMARELTKKFEEVARCSLKEAVAKYENESPRGEF
                     VLIIEGISEEKVLEEKKSEWDAMTVEEHYEMYIAKGFEKKEAMRKVAQDRGISRRDVY
                     NKLHE"
     gene            complement(886..>1542)
                     /locus_tag="PLA01_10325"
     CDS             complement(886..>1542)
                     /locus_tag="PLA01_10325"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HPD01729.1"
                     /translation="VLLANFADVKKNDTVIDLGSGTGIIPILIAGKTEAKSVIGLEIQ
                     EEMAEMAQRSVSMNGLDNRVRIVCGDIKESVELFGASRFNTVVSNPPYMNCGAGLINT
                     GDSKAISRHEIKCTLEDVIKAAAKLLVTGGQLAMVHRPNRLVDIVWLMRKYSIEPKYM
                     RFVHPSPYKKANLLLIKGTRQGGVELKMLEPLYVYDENGEYSGEINDIYCRGVRDIGQ
                     "
BASE COUNT          397 a          420 c          245 g          480 t
ORIGIN      
        1 gagtagagtc tccacctgaa gccccgatgt ttagctttag ctaaacgagt tcactcatgc
       61 agtttattat aaacatctct cctgcttata cccctgtcct gggcaacctt tctcatggct
      121 tctttctttt caaaaccttt tgctatatac atttcatagt gttcttccac agtcatggca
      181 tcccattcgc tctttttttc ttctaaaacc ttttcctcac tgataccttc aattatcaga
      241 acaaactctc ctcttggaga ttcattttca tactttgcaa ctgcttcctt aaggctgcac
      301 cttgccacct cttcaaactt tttggtcagc tcccgggcca tggttatcct tctgtctccc
      361 agagtctcat acaaatcctt taacgtgtaa ataagtttat ggggtgcctc ataaagaacc
      421 atagttcttg tttcatccct caaagaattg atccggtcct tcctttgcct tttgttcatt
      481 gggagaaatc cctcaaaaac aaatctgcct gcggaaagcc ccgacaaaac caatccggtc
      541 acagcagcaa caggtcccgg aaccatggtt acctgaatgt cattttcaat gcagagcttt
      601 acaatatcct ctcccgggtc tgaaatcccg ggtgtgcccg catcggaaac cagggcaatg
      661 ctttttccgt ccagcagttg tcccaccaaa tagttgccct ttacaatctt attatgctca
      721 tagtagctta ccaaaggctt ttttatatca aaatgattta aaagcttcaa agtctgtctc
      781 gtatcctctg cagctatgaa atctacctct ttcaataccc tcaaagccct tagcgttata
      841 tcctcaaggt ttccaatcgg agtggcaaca agatacaata tccctttatt gtccaatatc
      901 ccttactcct ctgcaataaa tatcgttaat ctcaccggaa tactctccat tctcgtcata
      961 aacatacaat ggctccagca ttttcagctc caccccaccc tgccgggtac cctttatgag
     1021 aagcaaattt gcctttttat agggtgacgg atgaacaaat cgcatatact ttggctcaat
     1081 ggagtatttt ctcataagcc atactatgtc cacaagcctg ttgggtctgt gaaccatagc
     1141 caactgtcct ccggtgacca aaagctttgc agcggccttt ataacatcct ccagagtaca
     1201 ctttatttca tgtcttgaaa ttgcctttga atcccctgta ttgataagac ccgcaccaca
     1261 attcatgtat ggggggtttg aaacaacagt attaaaccgc gaagccccaa acaattcaac
     1321 actttccttt atatcgccgc aaacaatcct gacgcggttg tccaagccat tcatgcttac
     1381 acttctttga gccatctctg ccatttcttc ttggatttcc aacccaatta cagacttggc
     1441 ttcggttttt cccgcaatga gaataggtat tattcccgta cccgaaccga gatcaataac
     1501 cgtgtcgttt tttttgacat ccgcaaaatt tgcaagcaac ac
//
LOCUS       DAOOJC010000470         1542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_66310_length_1542_cov_2.987223, whole genome shotgun sequence.
ACCESSION   DAOOJC010000470 DAOOJC010000000
VERSION     DAOOJC010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66310_length_1542_cov_2.987223"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..462)
                     /locus_tag="PLA01_10330"
     CDS             complement(109..462)
                     /locus_tag="PLA01_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPD01730.1"
                     /translation="MKASDIKIILEADLLTGEENLNMDVFAACGSDLMSDVMAYVKDN
                     VVLLTGLVNPQVVRTAEMMDIKLIVFTRGKRPEETIVQMAKEKGITVMTTKDPMFIAC
                     GKLYSAGLKERGVWD"
     gene            complement(796..>1542)
                     /locus_tag="PLA01_10335"
     CDS             complement(796..>1542)
                     /locus_tag="PLA01_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HPD01731.1"
                     /translation="HRLSIQLRVNGLKPVTISLDDYFVDRELTPRDETGDYDFEALEA
                     IDLKLFNQHLADLISGKEVDIPIFNFPKGCREDFCRKLKITENQILIIEGIHGLNEKL
                     TASIPKESKFKIYVSALTSMNIDDHNRIPTTDTRIIRRIVRDFQFRGCSAANTIKRWP
                     SVRRGEEKNIFPFQEEADVMFNSALIFELGVLKTFAEPLLADIDISQPEYSEARRLIE
                     FLTNFLPIETKEIPINSIIREFIGGSCFYK"
BASE COUNT          433 a          365 c          241 g          503 t
ORIGIN      
        1 ggtcaacacc aagctgtata agcatttttt ttacactgct cgatgcttct ccggcaacag
       61 taaaatcatc tgcaggtata acaaagtttc gccttattat accggtattc aatcccaaac
      121 acctctttct ttcaagccgg cactgtacag cttgccgcac gctataaaca tggggtcttt
      181 tgttgtcata acagttatcc ccttttcttt tgccatttgt acaatcgtct cttccggcct
      241 tttacctcta gtaaaaacaa tcaactttat atccatcatt tctgcagttc tgaccacctg
      301 aggattaaca agcccggtta acagaacaac attatcctta acataagcca tgacatcact
      361 cataaggtct gatccacatg ctgcaaaaac gtccatgttc aagttctctt caccagttaa
      421 aagatctgct tctaaaataa tcttaatatc actagccttc atacaagcac cttacccttt
      481 actattaaat atagtctaat ataattatag cagattgtta gagtgttaac aatctatatt
      541 gttattgaat taacaatcta ttatagtcaa aagctcaagg acttcgaacc cttgagcctt
      601 ttgataattt cacttcgtga aaccccacta gcaaaagctc cgggggcatc gagcccccat
      661 cgctttttgg tcatttcact ttgtgaaatc ccactaataa tgttccggaa acttaaatcc
      721 cgggaacctt tctcttcacg tcaatatatg cacccgatac cattgtgcta tgtcaaaccg
      781 tttaattaag cggatttact tataaaaaca gcttcctccg ataaactcgc gtattatgga
      841 gttgattgga atctcctttg tttctatagg taaaaagttc gttaaaaact caatcaacct
      901 tctggcctcg gaatactccg gctgcgatat atctatatcg gccaacagcg gttctgcaaa
      961 cgttttcaaa acacccaact caaatataag ggccgaattg aacattacat ccgcctcttc
     1021 ctggaagggg aatatgtttt tttcctcccc tcttctgaca gaaggccacc tttttatcgt
     1081 gtttgcagca ctgcatcctc tgaactgaaa atccctcact attcttctga tgattcttgt
     1141 atccgttgtg ggtatccggt tgtggtcgtc aatgttcatc gatgtaagtg cactgacgta
     1201 aattttaaat ttactttcct ttggtatcga agctgtcagt ttttcattga gcccatgtat
     1261 tccttctatt ataagaattt gattttctgt aattttcagt ttcctgcaaa aatcttctct
     1321 acatccctta ggaaaattaa aaatcggtat atcaacctct ttaccggaga tcaagtctgc
     1381 tagatgttga ttaaatagtt ttaaatcaat ggcctcaaga gcttcaaaat cgtaatcccc
     1441 tgtttcatcc cttggcgtaa gctccctgtc aacaaaataa tcatcgaggg atatggtaac
     1501 gggtttcaat ccattcactc tcagctgtat cgagagccta tg
//
LOCUS       DAOOJC010000471         1536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_66660_length_1536_cov_3.804186, whole genome shotgun sequence.
ACCESSION   DAOOJC010000471 DAOOJC010000000
VERSION     DAOOJC010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66660_length_1536_cov_3.804186"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLA01_10340"
     CDS             complement(<1..661)
                     /locus_tag="PLA01_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPD01732.1"
                     /translation="MGKTSLYLGIDVGSVSTNLVVINENDEVVDKLYLRTSGQPINAL
                     VKGMKTLSERIGNNVVVKGVGTTGSGRQIAGVIVGADIVKNEITTHAIAAQKLVPGVR
                     TILEIGGQDSKIIILKNGIVYDFAMNTVCAAGTGSFLDRQAARLEISIEEFGSYALRS
                     KSPVRIAGRCAVFAESDMIHKQQTGHSVEDIINGLCEALVRNYLNNLAKGKDIEEPIV
                     FQ"
     gene            complement(630..>1536)
                     /locus_tag="PLA01_10345"
     CDS             complement(630..>1536)
                     /locus_tag="PLA01_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA protein activase"
                     /protein_id="HPD01733.1"
                     /translation="LTTGGRGPCRFGYYCEMSREILHDNGFNMEVISLEVPQDGFTEL
                     LRRIRRIAGGFNAYKILKVIKNTTIVAKKVDELERLTYKFRPREIKKGSTDRIYKEFQ
                     KKVRDVRGSMQILKLVDDTKSQLMRLEIDREYKPLKVGIVGEIYTTIDPFTNFHIESI
                     LGNMGIEVDRTNTVSGWIIEHMLKAVVPFKKDIRYKEAAKPYLGTMIGGHAQETVGNT
                     VLYAKDGYDGVVQIYPLTCMPEIVAESILPAIEKDYNIPVLTLIIDEMTGEAGYLTRI
                     EAFADLLNRRRERTEIGQNFALSGN"
BASE COUNT          373 a          409 c          258 g          496 t
ORIGIN      
        1 cctgaaatac tatgggttct tcgatatcct ttcccttggc cagattgttc aaatagtttc
       61 tcacaagagc ttcacaaaga ccgtttatta tgtcttcaac actgtgtccg gtctgttgtt
      121 tgtgtatcat gtccgattcg gcaaacaccg cacatcttcc ggcaattctg acaggagact
      181 tggatcttaa ggcataagag ccgaattctt ctatagatat ctcaagcctt gccgcctgtc
      241 tatccaaaaa cgagcccgtg ccggcagcac agacggtgtt cattgcaaaa tcatacacta
      301 taccgttttt aagaattata atcttggaat cctgaccacc tatttccagt attgttctta
      361 caccgggaac cagcttttgt gccgcaatag catgagtcgt tatttcattt ttaactatat
      421 cggcacccac tatcacaccg gcaatctgcc ttccgcttcc agttgtgccg acacctttta
      481 caacaacgtt atttccgatg cgttcggata aagtcttcat accctttacc agtgcattga
      541 tcggttggcc gctggttctt aaatacagct tatccaccac ctcgtcgttc tcattaataa
      601 ccacaaggtt tgtgctgact gaacccacgt caattcccag ataaagcgaa gttttgccca
      661 atttcagtcc tctcccttct tctgttgagt aaatctgcaa atgcctctat tctggtcaaa
      721 taacctgctt cccccgtcat ttcgtcaatg attaatgtga ggacaggtat gttataatcc
      781 ttttctatgg caggaagtat actttctgca acaatttctg gcatacatgt cagcggatat
      841 atctgcacca caccgtcata cccgtctttg gcatatagca ccgtatttcc tacggtctcc
      901 tgggcatgtc cccctatcat ggttccaaga taaggcttcg cagcttcctt ataccttata
      961 tcttttttga agggaacaac tgcctttagc atatgctcaa ttatccagcc gctgactgta
     1021 ttagtcctgt ccacttcaat tcccatattc cccaggatag actcaatatg aaaattggta
     1081 aaggggtcta ttgtcgtata tatttctcca acaattccaa cctttaaagg cttgtattct
     1141 ctatcgattt ccaacctcat cagttggctt ttcgtatcgt ctacaagctt aagaatctgc
     1201 attgagcctc ttacatccct gacttttttc tgaaactcct tataaattct gtcggtgctc
     1261 ccctttttaa tctcccgggg ccggaactta taggtcagcc tctccagctc atccaccttt
     1321 tttgctacaa tagtggtgtt ctttattact tttaatattt tgtaagcatt aaagccgcct
     1381 gcaattctcc ggattcttct tagcagctcc gtaaagccat cctggggaac ctccaggctg
     1441 ataacctcca tattgaatcc attatcatgc aagatttccc tgctcatctc acagtagtac
     1501 ccaaacctgc aaggacctct ccctcccgtt gttaaa
//
LOCUS       DAOOJC010000472         1503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Acetivibrio sp. isolate AMR_MDS_2763
            NODE_68938_length_1503_cov_3.172652, whole genome shotgun sequence.
ACCESSION   DAOOJC010000472 DAOOJC010000000
VERSION     DAOOJC010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864838
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetivibrio sp. (wastewater metagenome)
  ORGANISM  Acetivibrio sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Acetivibrionales;
            Acetivibrionaceae; Acetivibrio.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:23:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,050
            Genes (coding)                    :: 2,022
            CDSs (with protein)               :: 2,022
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 8 of 28
            Pseudo Genes (incomplete)         :: 14 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Acetivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68938_length_1503_cov_3.172652"
                     /isolate="AMR_MDS_2763"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872092"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="PLA01_10350"
     CDS             <1..142
                     /locus_tag="PLA01_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HPD01734.1"
                     /translation="AMYISRLVKPLGINTTRIAHGIPVGGDLEYADEVTLAKALEGRR
                     EI"
     gene            304..>1503
                     /locus_tag="PLA01_10355"
     CDS             304..>1503
                     /locus_tag="PLA01_10355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HPD01735.1"
                     /translation="MEKLTLQKLESTLWKAADILRGNLSAAEYKDYIFGLLFLKRMND
                     QFESEKQSKRNEFTTKGMDEKEVEELLEDATIYETFFVPKSARWDKLKNLNLNIGPEL
                     DKAFKAIEDEPKNSELIGVLTTANYNDKERVPDKKLSQLLILFDSINLSDENLDSSDV
                     LGDAYQYLIKQFADEGGKKGGEFYTPSEVVTVLVNILKPQEGDRIYDPTVGSGGMLIQ
                     SLAYINKQGGNWKNVSLYGQEINLSTWAICKMNMLLHNAKGADIQKGDTIREPMHLEG
                     GVLKTFDKVLANPPFSLDNWGYNEAQADPYHRFPYGVPPKSYGDFAFVCHMVASLNNK
                     GKLGTVIPHGVLFRGGAEGNIRKGFLKDDLVEAVIGLPSNLFYGTGIPAALLIINTTI
                     IIITPIIS"
BASE COUNT          496 a          235 c          368 g          404 t
ORIGIN      
        1 tgctatgtac atttccaggc tggtcaagcc tttagggata aatacgacaa ggatagcaca
       61 cggaataccg gtgggcggag atttggagta tgcggatgag gtaaccctgg ccaaggcttt
      121 agagggcagg cgggagatat agagcagtat ttttataatc cgaagaagtt tcgtcataag
      181 tcatggggaa aatgtaaaaa ataacatttt accgtggctt tttatttggt ttgatgtata
      241 agttataggg taaaaggttt gataggaaaa taaagataga ataatatgag agggtaaggg
      301 taaatggaaa agctgacact gcagaaattg gaatcaactt tatggaaagc ggctgacatt
      361 ctaaggggta acttgagtgc agcagaatat aaagattata tatttggatt gctgttttta
      421 aaaagaatga atgaccagtt tgaaagtgaa aagcagtcga aaagaaatga atttacaaca
      481 aagggaatgg atgaaaagga agtagaggaa cttttagaag atgcaactat atatgaaacc
      541 ttctttgttc ccaagagtgc cagatgggac aaattaaaga atttaaatct taacattggt
      601 cctgagttgg acaaggcatt taaagcgata gaagatgagc cgaagaattc ggaattgatt
      661 ggagttttaa ccacagcaaa ttacaatgat aaagagcggg ttccggacaa gaaattgtcg
      721 caattactga tcttatttga ttcaataaat ctttccgatg aaaaccttga tagtagtgat
      781 gtacttggag atgcttatca atatttaatt aagcaatttg ctgatgaagg cgggaaaaag
      841 ggcggagaat tctatactcc ttctgaagtg gttacggttc tggttaacat tttaaagcct
      901 caggaaggcg acaggatata tgacccgaca gtcggtagcg gtggtatgct gattcagtcg
      961 ttagcttata taaataagca gggtgggaac tggaaaaacg tctctttata tggtcaggaa
     1021 ataaatctat ccacgtgggc aatttgtaaa atgaacatgc ttctccacaa cgccaagggt
     1081 gcggatatcc aaaagggtga taccattagg gagccgatgc accttgaagg cggtgtttta
     1141 aaaacttttg acaaggtgct tgcaaatccg cctttctcgt tggataattg gggctacaat
     1201 gaggctcagg ccgacccgta tcatagattt ccctacggtg taccgcccaa atcttatgga
     1261 gacttcgcat ttgtatgcca catggttgca agccttaaca ataaaggaaa gttgggaact
     1321 gtaatacctc atggagtatt gttcagaggc ggggcagaag gcaatattcg caaaggtttt
     1381 ctgaaagacg accttgtaga agccgtaatt ggccttccca gcaatttgtt ctacggaaca
     1441 ggtattccgg cagcactgct aataataaat acaacaataa ttataattac cccgataatc
     1501 agt
//
